LOCUS       DAOTLW010000001       106408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_160_length_106408_cov_10.482817, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000001 DAOTLW010000000
VERSION     DAOTLW010000001.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 106408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 106408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..106408
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_160_length_106408_cov_10.482817"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            155..889
                     /locus_tag="P5516_00005"
     CDS             155..889
                     /locus_tag="P5516_00005"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT90853.1"
                     /translation="MNQKENPQSVIDKYSKRQKYMPYVLGGLAGILALAGIFIIISVA
                     TGSGNLFKGMFATKTPTPTATFTPTATVPSPTPSMTPTITETPTPAATDTPSGPQFYT
                     VKQGDDCWTLANTFGVDLLVLLAINNFPSGTCPLVPGTDIIIPAPGQTLPTSTPLPAD
                     IPRGTEIEYTIQLGETLQDIASRFNSTLEDIKTRNNIKNENLIYVGQVIKVRVNLVTA
                     TPTVAPTSTLSLFTLQPTKPAVTTTP"
     gene            complement(954..2009)
                     /gene="queG"
                     /locus_tag="P5516_00010"
     CDS             complement(954..2009)
                     /gene="queG"
                     /locus_tag="P5516_00010"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HRT90854.1"
                     /translation="MSQIPSSLESEIKARALSLGFTVCGITTPDTLDFHHMYVNWLER
                     GLNAGMTYLASAYHVSNRLHPEKFFPGLKSIILLGYRYPLQHPAEIEEKTTGLISGYA
                     SGKDYHHLIPNLASPLLQYLTALNPEAPAPRVYTDSAPVLERELAVRSGLGWIGRNSC
                     LISPKHGSNLLLAEIFTAIALQADPPFAADHCGSCMRCVESCPTGCILPERVIDANRC
                     LSYHNIENKGMIPPEVMEKFGGWIFGCEICQMVCPWNQRGYQSSSSIGIAGQMTVEEM
                     LEFLELSMDDFKSRFAETALARARYNGLKRNVIIRLANLGEKNALGSIKRTEDTAELD
                     WLRDTARWARNKIQLKK"
     gene            complement(2027..2734)
                     /locus_tag="P5516_00015"
     CDS             complement(2027..2734)
                     /locus_tag="P5516_00015"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HRT90855.1"
                     /translation="MLKRRMLFNFIIVIVIGMLLVSCKGKPSIDPNQKMTEVAATVQA
                     GLTQAAASNPTATPTLEPTATATPSPQTPTPLTAISSPVTVLVPTQPQTSTADNALFI
                     TDVTYPDGSVVKPGEHIVKTWKLQNTGKTTWTKEYSVLYLEGVLYGAGSQLMFRLPAE
                     VKPGDFAEISVPFIAPTTPGNYGSFWKMYNSSGYVFGDPFNISIIVGEPTPTGNAPTA
                     TITPTITGTVSAGWGTP"
     gene            complement(2826..3851)
                     /locus_tag="P5516_00020"
     CDS             complement(2826..3851)
                     /locus_tag="P5516_00020"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRT90856.1"
                     /translation="MKKPSGIPQRWAVIGFIISVFFISAAAPVNNLPFSTNVNSSSQG
                     DALRLGDTPEDPYYPSNKPFFQAPLSLTPHDHFWLSRPIRDEILTWRTADYRYGYFIK
                     QESIVHGGLDIPAPEKTPVLAAADGTVVFSGYGLLYGEGARNDPYGIAVMVRHDMELD
                     GDTLYTVYTHLGKTLVKVNELVKAGQKIGAVGMTGNTSGPHLHFEVRVKGKNGMLLQN
                     PELWMVPPLEHGVLAGRIINNMGNLLANWNIRLTSLDTGTVWRIRTYDGSIVEESQQD
                     QYYQENFVLNDLPAGRYEILTYYNASAYKYEVTIVPGAVTYINFLGKKGFSLGNPPGS
                     QNTEFLN"
     gene            complement(3848..5602)
                     /locus_tag="P5516_00025"
     CDS             complement(3848..5602)
                     /locus_tag="P5516_00025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90857.1"
                     /translation="MNTSARLCTRLILLIPFLVVSLSACKPEPAAPEIAAHVEGTVVS
                     LPEPSETASPLLSTPTLIPTTSPGSVATSENPATSGLIILAVKIGGFAQLAAYHPLNQ
                     ALTQITDDEWDHADPAISPDGTKLAFCADEFGHWDIFILDLPTGEKTNLTNATTYACS
                     PTWSPDGRWVAYEVVMDGKLNLVIRSVMDESVAPHRLTDNGGNNFDPAWSPSGREIAF
                     VTDRNGRLELWLANLDNPQQRFNLVARSEHADYTHPVWSGDGHSLAWVRTDEFEEIEA
                     WNLESEQTRPVTMGSGSHPVWMDEEQGVLAVVHSANSNDLVAYSTEPNLLLLPPIHQK
                     NEVLSISWLPAKSALPLKKYLSQHSPASETRLWQPEISTQDVSSGRYEMVYLPDVTAP
                     EPYLIDTVNESFNSMRAALRSKLGWDFLKILESASISIPAYSSSGLREDWGYTGRAIT
                     VNLDPLDSGWMAVSREDILGRTYWRVWLKCMQQDGTCGVPIQNPVWDFNARIQGSSSA
                     YENGGLATTLPQGYWIDFTSFALGYGWERLPAGSNWQVYFPETRINRFVLRDGLSWHE
                     AMRQRFTDTTIESYWSLP"
     gene            complement(5646..6524)
                     /locus_tag="P5516_00030"
     CDS             complement(5646..6524)
                     /locus_tag="P5516_00030"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRT90858.1"
                     /translation="MKKQRAVILCLTLSFVLVATGCSSSFPQPPTPTPVMTTPTFHAT
                     TTPSPSHTATQNPTPAFTATLQPAIKACSPLKDIELSDLHKIMSQGFTPPAPYKDDGH
                     PAIDLAFFTFKELPSMIGHPVQSIFPGIVNLVIVDRFPYGNAVLIETPLNVLSAEWVS
                     ALPIPTAIPQENLDLVRPCDSDPLFADMTPIEWDGSRKSVYVLYAHLLEKPSVRAGDV
                     VNCGQVIGAAGNTGNSAAEHLHLEARVGPAGAQFGTLAMYSPDATIEERYRYCIWSSS
                     GRFQSFNPVILWEQEP"
     gene            complement(6534..6869)
                     /locus_tag="P5516_00035"
     CDS             complement(6534..6869)
                     /locus_tag="P5516_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90859.1"
                     /translation="MNTPHNSPKVTAPPQIDRLQAKLLPDNQRVRVTLVLNNVECRPT
                     LELSLLDEKQMEIARSTIIGTFNPLVSFTLHLGQHSPNDRLFLQAVVFPNDNEFSDSK
                     KVPVEVGSQ"
     gene            complement(6896..8215)
                     /locus_tag="P5516_00040"
     CDS             complement(6896..8215)
                     /locus_tag="P5516_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1015 domain-containing protein"
                     /protein_id="HRT90860.1"
                     /translation="MRNYPEIGVQVPEIYLPVEKIDLTKWAVIACDQYTSQPEYWEQV
                     AKNVGDCPSTFHLILPEVYLGTDKETERVQSTHAKMQQYLSDGLLRPQQNIIYVERTI
                     GVKTRRGLMLALDLEHYDFSKGSQSLIRATEGTILDRLPPRIRIRKNAPLELPHIVVL
                     IDDPEDRVIGPVVQASQRLPLAYDFDLMQRGGHLRSRLVNDSALEKSIIQNLAALANP
                     QTFYAKYRVSPDKGVLLFAVGDGNHSLATAKAIWEELKSSVSMDHPARFALVEVENIH
                     DAGLAFEPIHRVLFNSKVNLEQVLKGIYDGALTLEPVSSFTELTTKVKQPSVECQRFG
                     LVSTRGLVAGSITSPTANLVVGTLQTILDQLVADGSVASIDYVHGDEALCELGMKPAN
                     TGIYLPGMSKDELFRTVMLDGALPRKTFSMGEAHEKRFYMESRKITI"
     gene            complement(8312..9172)
                     /locus_tag="P5516_00045"
     CDS             complement(8312..9172)
                     /locus_tag="P5516_00045"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRT90861.1"
                     /translation="MNKVMVVTDSTANIPPELWDGNPIVSVPLQVIWGNEILRDGVDI
                     QAKEFYERLAVADVMPSTSQVTPEEFKAMYGRLIEEGYDILSIHISSKLSGTLDSAIQ
                     AKNAFSGARIELVDSLSTSMAMGFQVLSAARMAGIGATLEECKAVAEKAKANTGVYFV
                     LNTLEFLHRGGRIGGAQAFLGTMLNMKPILELRDGRIEAVERVRTIGKATERLLDLVE
                     QRVGTAQGPVRLCVVHANAEKEGQALLERAIKRFPSSLVTDAVLSTVSPVLGTHAGPG
                     ALGVAFMHSM"
     gene            9354..10676
                     /gene="ffh"
                     /locus_tag="P5516_00050"
     CDS             9354..10676
                     /gene="ffh"
                     /locus_tag="P5516_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HRT90862.1"
                     /translation="MFENLTGRLNQIFTQLRRKGKLSAADVDAMMREVRLALLEADVN
                     YTVVKDFTTRVRERAIGAEVSKALNPAQQVIKIVHEELIHTLGEPARLNLSGVKPYAI
                     MLVGLQGSGKTTAAAKLAKRLRSDGERVLLVAGDPYRPAAVKQLQTLGEKIGVEVVAD
                     STLKPPALAAHAFDKAQKGGYSVVIIDTAGRSQLDQTLMDELQSIAKKVRLVETLLVV
                     DAMIGQEALHVAEGFRDTVNITGLVFTKIDGDARGGAAISVRKVTGVPIKFLGTGEGI
                     DAVEVFDPARLASRILGMGDMIGLIEKAEAAFDEKTQKEQAERVLKGQFNLEDFADQL
                     KQLRKMGPISQVMDMLPGGLGQASRNIPPEETERNLKRTEAIIASMTKQEKRDPDLLN
                     ASRRRRIASGSGTNVMEVNRLIKQFRDVQRLMKTMQKTGGKGLSRLFK"
     gene            10784..11119
                     /gene="rpsP"
                     /locus_tag="P5516_00055"
     CDS             10784..11119
                     /gene="rpsP"
                     /locus_tag="P5516_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HRT90863.1"
                     /translation="MVRIRLRRTGQRHQPSYRIIVADKESPRDGRFLEIIGHYIPTTD
                     PFTFEVQEDRVYEWMKNGALPSDSVLKLFKIVGVLERYERFKKGESLETLLAEAKKAT
                     EERNAKIKA"
     gene            11128..11427
                     /locus_tag="P5516_00060"
     CDS             11128..11427
                     /locus_tag="P5516_00060"
                     /inference="COORDINATES: protein motif:HMM:NF024483.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HRT90864.1"
                     /translation="MMQPTNRPTQRYSRNVNQESHIKELVEYIALSLVDDPSQVVVTE
                     YRSGGRENLELHVAREDMGRVIGKGGKIANAIRVLLRVAAAREGKQVNLDVAEPR"
     gene            11424..11984
                     /gene="rimM"
                     /locus_tag="P5516_00065"
     CDS             11424..11984
                     /gene="rimM"
                     /locus_tag="P5516_00065"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HRT90865.1"
                     /translation="MTNRKSTGPQLKTQPGSPQPGEPVYLMIGKLRRPHGVKGELWLE
                     LTTEHPERIRTGVTILIGDEKTEVKVENIRAADKLWLISFAGYQDCQEVEGFRNQWVF
                     IEAKQAAPLPAGRYYHHEIIGMKVFTETHDLIGVVDEILVTGANDVYVVKNDKGTETL
                     VPAIKSVILEIDKEMGRMIVRQQEWL"
     gene            11994..13106
                     /gene="dprA"
                     /locus_tag="P5516_00070"
     CDS             11994..13106
                     /gene="dprA"
                     /locus_tag="P5516_00070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HRT90866.1"
                     /translation="MEPDKYWVGFNLVKGIGAVRFKQVLDFFGSAETAWFAPVSGLQA
                     AGLPQRVIENFLLVKSQVDLERVMERIHHSGTRVITWNDQDYPRRLKEIDQSPPVLFI
                     RGSINVEDDWAVAVVGTRRVTPYGRQVANEIATFLGQNGVTVVSGLARGVDAIAHQSA
                     LAAGGRTIAVLGSGVDVIYPPEHRKLAVEIIAQGALVSDYPLGTSPDGVNFPPRNRII
                     SGLSLATVVVEAGEKSGALITAEFAVEQGRDVFAVPGSILAPQSAGTNQLIEQGARPL
                     LKMAEILEVLKLEQIPEKQQARKLNPLNQAEQNLLQHLSTEPLHIDQLCELTGLPIHD
                     VSATLTMMELKGFICQVGGMNYVAMRENRGLYKVNK"
     gene            13103..14197
                     /locus_tag="P5516_00075"
     CDS             13103..14197
                     /locus_tag="P5516_00075"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRT90867.1"
                     /translation="MKIEHYYAVIMAGGGGTRLWPLSRHDSPKQTLPIFEGKSLFQLA
                     VERLQGYLPVNQIYVVTSADLMEQLHAQVPELLTSNFLIEPQPRGTAAVVALAAATIL
                     ERDPNGVLAVLTADHLMPDKAEFQACLQAAAELAEQGYIVTMGIKPASPATGYGYIER
                     GAELGVYSGRVGYEVLKFIEKPHQSKANELIKQERYSWNSGMFICQAQVVLGEFERYM
                     PELYTVIQSLQPFLASDHLAAPFAVKWLKIQPETIDYGIMEKTSLAAVLPLSTLEWID
                     VGSWDSFFEVFKPDENGNIILGVKYEGIDTADSLILSTDTERPVVTMGMKNMIVIQAP
                     DVLMICPRGESQRVKELVNYLKASDYTLIL"
     gene            14209..16512
                     /gene="topA"
                     /locus_tag="P5516_00080"
     CDS             14209..16512
                     /gene="topA"
                     /locus_tag="P5516_00080"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HRT90868.1"
                     /translation="MEAYCVKCKQKREIESPNAEFNALGTPVTRGKCPVCNTSLYRIG
                     ETPAHAGLERPKRLHAKPRSGKLVIVESPAKAKTVGRFLGKDYTVRASVGHVRDLLRS
                     ELSVDVENNFLPKYRVPNEKRPVVKELKALAKEAAEIYLATDPDREGEAIAWHLLEAA
                     QIEPKLSRRVVFHEITDHAINEAFSQPREINMDLVDAQQARRILDRLVGYSISPVLWE
                     KVRSRLSAGRVQSVALRLIVEREREIEVFVPVEYWSIHAELTPEGKKTSYMTKLAKID
                     EVDPDLGNKEVVDGYLADIEASPFAVTRIKKSERRRKPSAPFITSTLQQEASRRLGYT
                     ARRTMMIAQQLYEGLEVGEGGATGLITYMRTDSTNVAEVAQEEARSFIQQTYGAGFLP
                     EKPPQYKTRAVSAQEAHEAIRPTSVLRQPEKIKEFLSSEQSKLYQLIWQRFVASQMES
                     AVYDTISVEVTATGKDHQYLFRASGSTIKFPGFLIVYEETVDEDLKTEETENVRIPAG
                     LVEGQAQHLKRLIPEQHFTQPPPRYTEASLVQVLEEYGIGRPSTYAPILSTIQQRGYV
                     LREAKRLSPTETGILVNDLVVEHFPDIVDLNFTAGMEAHLDQVAAGKEDWHKVLADFY
                     TTFEPQVKRAQAEMPVTKTEPEKIGRACPNCGHDLVIRWGRYGKFISCSNFPACRHTE
                     ALLEKIGVTCPKDDGDVVLRKTRKGRIFFACSNYPNCDFTSWKRPLPVPCPKCGGWMV
                     VANKREAQCMVCEESVLIEELGVPIPD"
     gene            16576..17889
                     /locus_tag="P5516_00085"
     CDS             16576..17889
                     /locus_tag="P5516_00085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90869.1"
                     /translation="MDKLRDVFFKLIDKPLYTAIAGFVIGLIIGLPILGWWLWPVKWK
                     DADASFLRQDLKNQYLCMVVDSYSVNHDAALAGARINSLGVDIKKNPFLFDNLAAGGC
                     NYAPTDKVIQSLKTALVTGEQVVPETEANNQPDGDQAEIKATEPLATAEIAGSTAIPL
                     PGNNAPKEKKGGSVLLLSLLCVVFLLIGGALIYIFFIRNRRTSEEIPHAWDEEERFAQ
                     DEPYDDAPEYPAAATPPPPTAIHHESVDTPPMDYFSEPEEMDDVDEMEIPDILEGAQD
                     VAKTPPLKSPGVRATLPHHSDPVAHFMTTYVIGDDLYDDSFSIDAPNGSFLGECGVGI
                     LDTIGVGDPKKVTAFEVWLFDKNDIQTVTKVLLSAHAMNDAGVKQRLASKGELVQIES
                     GQQLLLETATLQLEARVVEMVYGQGALPASSFFQRMTLELSVWPK"
     gene            complement(17933..18400)
                     /gene="greA"
                     /locus_tag="P5516_00090"
     CDS             complement(17933..18400)
                     /gene="greA"
                     /locus_tag="P5516_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRT90870.1"
                     /translation="MNQPSYLTREGAEKLRAELKHLEGPIRQELAKRLRAAIQMGDLS
                     ENADYAAAKEEQAFVEGRIQELTQILRDVVLIEDGPSHTGKIQIGSTVTIQEDQEEEE
                     TFHIVGPQEADPRRGKISYDSPIGQALLGHQEGDRITANTPAGELHFRILKVK"
     gene            complement(18428..19579)
                     /locus_tag="P5516_00095"
     CDS             complement(18428..19579)
                     /locus_tag="P5516_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HRT90871.1"
                     /translation="MKFDRAYLEKIEEETLATYAVHASRSKGRKYPENEPLYRTCFQR
                     DRDRVLHTTSFRRLEYKTQVFINYEGDYYRTRLTHTLEVAQIGRTIARAMGANEDLVE
                     TICMAHDLGHPPFGHSGETTLNQLMEGYGGFNHNHQSYRIVTELEHRYAQFPGLNLSW
                     EVLEGIVKHETEYDKTDALDYDPHLRGHLEAQITNVADELAYTAHDLDDGLRSGMITT
                     DMLSEIDLWNMVMERLNIHSRQLDDLTRHSIIRELIGLEVDSMISESNERLQRSGVKT
                     LADLQSHAQNVVGFDDVMRARNRELKDFLYKNMYRHYRVVRMQKKAERILSELFHAYV
                     SEPNMLPIHFQDQLEIKGKEKTICDYLAGMTDRFAVDEYQKLFDPSLLP"
     gene            complement(19584..20396)
                     /locus_tag="P5516_00100"
     CDS             complement(19584..20396)
                     /locus_tag="P5516_00100"
                     /EC_number="2.7.4.26"
                     /inference="COORDINATES: protein motif:HMM:NF040647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isopentenyl phosphate kinase"
                     /protein_id="HRT90872.1"
                     /translation="MTTPLFFLKLGGSLITNKNIPHQARSDVLKRLADEIARALTDQP
                     GVRLLLGHGSGSFGHIPAKKYQTRDGVATFAQWRGLVEVWHEARALNTIVMEVLHESG
                     VHAMAFPPSAQVITSSHKITHWDTTQIAACLDNGILPVVYGDVVFDQTIGGTILSTEE
                     QFEFLAAIFKPARVLLAGIEKGVWMDFPKRKELLDAITPRNLAMVDPHLESSESPDVT
                     GGMRSKVHNMMNLVTNLHCQQVLIFSGLDEGNVFQALTGRKVGTCITLDKKG"
     gene            complement(20396..21352)
                     /gene="mvk"
                     /locus_tag="P5516_00105"
     CDS             complement(20396..21352)
                     /gene="mvk"
                     /locus_tag="P5516_00105"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HRT90873.1"
                     /translation="MPAITSSSPGKVILCGEHAVVYGAPAIALPVFQLNTKTTILAQP
                     TAPEGKVRMIAPCIGLDSDLESLAYNHPLKATTALVMQKMGVTRLQACEIRINTTIPI
                     ATGLGSSASVTVSLARALSAFLGSPLSDELVNQVAFEIEKLHHGTPSGIDNTVVTYQV
                     PVYFVKGKPIEHLKIRQPFTLVIADSGAASLTAETVARVRERWLAEKKRYDAIFANIA
                     DLSREVKENLEKNDLNQHGELLSRNHELLQALGVSTPMLDDLVNCALQAGALGAKLSG
                     GGGGGNIIALVHHTQADSISRALEQQGAVRTITTTLAPGRGA"
     gene            complement(21360..22232)
                     /locus_tag="P5516_00110"
     CDS             complement(21360..22232)
                     /locus_tag="P5516_00110"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HRT90874.1"
                     /translation="MLKYILKAMRPRQWTKNAFIFAALVFDRKLLNLHAFLQTLAAFV
                     LFCLLSSSVYLINDILDRESDRAHPIKKNRPIASGNLPVGLALATGIFLLLVSLTGAF
                     LLSHGFFFISLVYFTLNLIYSIWLKHIPIIDVLVIASCFVLRVAAGVSVIEVQRFSPW
                     LYVVTTLLALYLGFGKRRAELAVLVNDSPQSHRKVLSGYSIQFIDQLITVVSATTLIS
                     YSLYTFSAPNLPENHSMMLTIPFVLYGIFRYLFLINKHNAGGEPEEILLKDRPIQLTV
                     LLWGVAVMVIFYLF"
     gene            22279..23118
                     /locus_tag="P5516_00115"
     CDS             22279..23118
                     /locus_tag="P5516_00115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HRT90875.1"
                     /translation="MQKTRLDILMESRGLSPSRSQAQRLIMAGSVRVNHQVVLKPGTK
                     VDPGDTIEISESPRFVSRGGEKLLSALQAFHLTSLADRVCVDVGASTGGFTDCLLQHG
                     AKKVFAVDVGYGVLDWKIRNDTRVVVMEKQNARYITTFHEPIDLVVIDASFISLRTLL
                     PVVRQWLNAPKGEVVALIKPQFEAGRSETARGSGVIRDPEIHRAVLRQVLEFAQKEGY
                     TAEGLIRSPLTGPKGNIEFLAHLIYPGETPADVERLIEGLPWNEGTTADQPCQPEPEG
                     KLK"
     gene            23137..24936
                     /gene="lepA"
                     /locus_tag="P5516_00120"
     CDS             23137..24936
                     /gene="lepA"
                     /locus_tag="P5516_00120"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor 4"
                     /protein_id="HRT90876.1"
                     /translation="MSTERIRNFCIIAHVDHGKSTLADRMLQLTGTISDREMTEQVLD
                     SMDLEREKGVTIKASAVRMNYVHAGETYELNLIDTPGHVDFNYEVSRALEACEGALLV
                     VDATQGIEAQTLANLYLAIESNLTILPVINKIDLVSARPDEVAEDIESLLGLPAEEII
                     RVSAKDGLNVDQVLDAIVTQIPPPKGDDTAALQALIFDSHYDSYKGVVAYVRVMQGII
                     NADTTLRLMATKADVKPVEIGVFAPGMIPVPLLSAGDVGYVATGLKTVKECRVGDTFT
                     LSSEPAAAPLPGYKHPKPMVFAGIYPVEGEDYEALKEALERLQLNDASLVFEPETSQA
                     LNFGFRCGFLGLFHMEIIQERIEREYNLNIVVTAPSVEYEVVEISGEVLRISSPARLP
                     DEALIAEVREPWMRLEVISPTEFYGVIMELTKKRRGIFVSQDYPSPNRVQLNYDIPLS
                     ELIVDFFDDLKSRTRGYASMDYHFLDYRPGNLVKLEILVDSEPVDALAAIVHKEDAYH
                     KGQALITRLKALIPRQLFDVAIQASASGRVISRANVKALRKDVLAKCYGGDITRKKKL
                     LEKQRKGKRRMKMIGDVEIPQEAFMAVLRLEDE"
     gene            24933..25955
                     /locus_tag="P5516_00125"
     CDS             24933..25955
                     /locus_tag="P5516_00125"
                     /inference="COORDINATES: protein motif:HMM:NF015651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRT90877.1"
                     /translation="MSMVRPNWKKQAARWLPGLLISAMAVYAIAHFTRGQHIGEALKT
                     VKIGYVLLLIFLTLLSLIARSIAWRAILGNKITLVNSFFGICEGYFLNNIFPLRVGEL
                     GRALFVGRSSGLGTLHVLSTILIERAFDIVFAASIILLTLPMVVGSEWIKPIAFSAFV
                     VILVGLVILFLISVKRQKFQTWIESKEVRIGFIKNRVLPQVSKILDGLSALVHPGQFL
                     LSVFWIGITWLLWVLLYYWTIAQLAPSAPLWWGAFTSGLIALGVAIPSAPASVGVYEA
                     TILGAFALLGISSGSAVAYAIVLHLVQIFVTAVFGLWGMARDGQKLSTLLDSLVNKKN
                     VENPTG"
     gene            25963..26988
                     /locus_tag="P5516_00130"
     CDS             25963..26988
                     /locus_tag="P5516_00130"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT90878.1"
                     /translation="MNGFTLITGGAGFIGANYAARCLARGEKVVIFDNLSRRGAELNI
                     EWLSDRFERRSFELVKEDVRDAAAIKTAARGADRILHLAGQVAVTTSVCDPRTDFEIN
                     AGGTFNVLEAARASGRNPVFLYASTNKVYGGMEDEQVIEQETRLDYLDHPHGINENQA
                     LDFHSPYGCSKGAGDQYTRDYFRIYGLPTVVFRQSCIYGSRQFGIEDQGWLAWFIIAA
                     VTGKPITIYGNGKQVRDVLFIDDLLDAYDAAFSRVEASRGQVFNIGGGRENSISVWLE
                     FRPILEELLGHSIKTRNREWRPGDQPVYISDIRKARSVLGWEPRVTVKQGIRRLFDWV
                     KENQNLF"
     gene            26985..28145
                     /locus_tag="P5516_00135"
     CDS             26985..28145
                     /locus_tag="P5516_00135"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT90879.1"
                     /translation="MKILIVLTYYRPHISGLTIYAERIAKAFARRGHQVTVLTSRFEK
                     NLAADELDEDVRIIRAPVLMRLSKGVIMPTFGWLASKLVKEHDVIQLHLPQFDAAGVA
                     LRGRLLKKPTIITYHCDLELPPGLFNRAANLAVLTMNELAAKFTHRIVTYTEDYARHS
                     WYTRRYMKKVKIIPPPVVLPRVSKTEISAFQAAYNPQAQRPVIGMAARFATEKGVEVL
                     LRAIPRVINEFPNALFQFVGPYKNIIGEEGYFARLEPQIKEFEQAGNWKFLGSMSPAQ
                     MAAFYANLDLLVVPSLNSTESFGLVQIEAMMNGVRSITSDLPGVRQPVKVHRMGSIFP
                     IGDADALARGILHALQNPQLIQEANTDFSAYDPDTTAAAYERLFNEITVELH"
     gene            28176..33464
                     /locus_tag="P5516_00140"
     CDS             28176..33464
                     /locus_tag="P5516_00140"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRT90880.1"
                     /translation="MVTDQPEKKKRANNGIRGNLVWDLLLIVVLLAGAIFRFTGIQWD
                     GNQHLHPDERFLTMVETGIAPVKSVSDYFDTATSSLNPNNRGYTFYVYGTLPLFIVRY
                     AGEWVGKSGYDQINVVGRVLSGVFDLGTILFIYLIARRLYRHARLGLLAAGFSALAVL
                     QIQLSHYFTVDNFANFFTYGALLTAVHIATSRTFSPVDKLKEKRWKNAPDWLTTGWRS
                     AWLYALFGLLYGMALASKVSIWALAALLPLAAWIYYKNLHEEEKAAALPVLIRNLALA
                     GVIAFVSFRVFQPYAFSGPGFFGLKLNPNWLSNMRELSTLMKGDVDVPYALQWARRPL
                     TFTIQNLVLWGLGVPLGVLSLSGLVWMTWRIIRGDWKKHILIWSWTLFVLVTQSLNFV
                     KSMRYIISIYPALALIAAWTIFKLLEKGSAVGRKRGLGHFNWGRILSWLTLVVTLLAT
                     TLWAAAFSSIYTKPVTRVAASEWIYENIPGAINLKISGLDGQIFTQPYAYQNTAVISS
                     ENPYRYQITAVKNGQVNSVSVQHVLLHSGSTLQNTLLLSIYEYGQNGKHLAGAGFTQA
                     DFSGLSDNSGDQALIQLQTPVPLEAGRHYELEFSVAEPGTFLRIGGSVAINTSQWRIY
                     LPQPVFQLTSASPYQLLIYPRETGKLDEIVLNRVADQSEENQVTRVKAFIYAPDDPQT
                     ILASAELVSRFANDPDLRGQPYTLKFDEAPLLEKNKGYSLLLQVEGSAVLGVYNSITA
                     LESTWDDALPVGLYGYNQFGYIDGIYGNHLNMELYWDDNAEKLERLTSILDQADALFI
                     SSNRQWGTIPRVPERYPLTSQFYRSLIGCPEQKDILWCYAVAKPGIFQGELGYKLTAV
                     FQNDPALGPFKINDQLAEEAFTVYDHPKVLVFEKTSAYDPDKTAKTLKSVDLTSVVHK
                     TPRQASRISGNLMLTAKEEDIQTSGGTWRELFPPESPLNRQPWLAAVTWYVVVMLLGW
                     TVYPLVRFFFRGLLDRGYGFSRLVGLLFLAYLTWMAGSMGLPSSRVTISLVFLLLLST
                     RVAAAALRRGEIWQEIRKNSRRFLIIEGVFLALFVLDLAIRIGNPDLWHPWKGGEKPM
                     DLSYFTAVLKSTTFPPYDPWYSGGYINYYYYGFVIVAVLVKWLGIEPAIAYNLILPTL
                     FAFTGIGAYTVGWNLFARLKLDDRSSPTGEKRSYQAGLAAAAAVLIAGNLGTLKMLWQ
                     GLQRLAAPGGIIDGAGFFERLGWFFSGVMRFVNGARLPYGFGDWYWNPSRAIPGDVIT
                     EFPFFTFTYADLHAHMIALPITLLVLGWVVSILFRNWEAHSSARFQTAAGLIITILFG
                     GFTIATLRPTNTWDLPTYFLLAVFVLFYSSYRNAQPPSGFLPAAPCWLRRILYSMIFI
                     GVLAAGVMLFYLPFTEKFGQAYGMIDVWKGDHSPLGSYLVHWGWQLFLIVSWFAWETR
                     DWLASTPASAIRRMKPYLGYLQVIGVLFTMVVALLTLLRVHIGWLVCILGVWALVLLL
                     RPHQADSKRLVLFMTGTALVLTLFVELFALQGDIGRMNTVFKFYFQAWTLLAIAASAA
                     LLWLIPAATSVWKSGISTAWQVLTVILIAGSLLYPLTASMDKIRDRMSASAPIGLDGD
                     AFMQSATYFDQGVEMDLNQDYQAVQWMRENVQGSPVIIETNTVEYRWGNRFTIYTGLP
                     GVLGWNWHQRQQRGFLDYNGINNRLNDIPLFYQTTDIEQALEILKRYQVKYIILGQME
                     RAYYSGAGLDKFANYDGLYWKEVFHEKDTVIYEMID"
     gene            33482..36055
                     /locus_tag="P5516_00145"
     CDS             33482..36055
                     /locus_tag="P5516_00145"
                     /inference="COORDINATES: protein motif:HMM:TIGR03662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HRT90881.1"
                     /translation="MSQAINIFVWYLVGTFVGVIALPLVFRFFPSLPSRGFALARPLG
                     LLIWGFTFWLLASFGVLQNDLGGQVLAVLILLAFSLAELKGTRKDALLTWLRQNLRTV
                     LTIEGLFLFVFLAWVLVRGMNPEVAYTEKPMELAFINSILRSTSFPPQDPWLSGYAIS
                     YYYFGYVMVAMLTRFSGVAAATAFNLANASWLAMTAVALYGIVFDLSLAGAWNSHHQT
                     AKQVEKFARRAGFLGPLFVLLLSTYEGVLEILHAGGLFWKVDPQGQLSSRFWNWLSIL
                     DLNQPPTQPFDWIPSRGGGWLWWRGSRVLQDLNLGGQHIEVIDEFPFFSYLLADLHPH
                     VLAMPFTLLAIALAMNLFFDGGTYLQQSRRLLDWARNENFWLIALVLGSLGFINTWDF
                     PIYVGLFCLIWSFKSYRATGWKPGLLAEFLKNGLALGVAGMVLYLPFYLGFGSQAGGI
                     LPSLEFMTRGIHFWVLFAVLLVPIIAFCLNQAKRKLGWSDFWKGARFTLALLSVLFIL
                     MVFYGALLLSFQAWGAGLSASSNLFLSALGVKMTQGGAAFAGVHAGYPAGEVLKASLL
                     RRLQAPGTWLTLAALLTITLSVLLKRKTAQSVEIPAEGEHLRITNGAEELIFFLILFG
                     LLLTIIPEFFYLRDQFGTRMNTIFKFYFQAWVFWGIAAASGSAVLFRQLRGWRGSVFQ
                     IMWILVVAGGLAYPVIMLTDKTNSFSMKGWTLDGNAYLQKFNPEEYAAIGWLQDAPMG
                     VVVEAVGGSYTDYARISTRTGLPTLLGWPGHESQWRGGGEEMGSRFADIQQLYETTDW
                     NEVWRTLRDYNVRYVYIGNLERSTYRVSPSKFDSHLPLVFSNSSTRIYEVTAPSGEAQ
                     P"
     gene            36052..37737
                     /locus_tag="P5516_00150"
     CDS             36052..37737
                     /locus_tag="P5516_00150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90882.1"
                     /translation="MNGNLDSRKAVPLELLVYLVVLVIAAALRFSNLGGLPLTDHEAS
                     LALQALRLSKGEQVLISGEPGYIALTTLWFSLFELSDFTARFWPALAGTLLALTPLLY
                     RERVGKAAALVLAGLVAVDPIMIGASRSAEGSMLALLGLLAAVGFAMRSKPLWCGFAL
                     GFALAGGPALWPGVICLAVFLLINCKSLRQVDNRFLALAAMTAMVTLVLLSTLFLINP
                     PGISAFGSSLVDYWGSWRSENPAPLKGIAAAWGSSTLLVLVLSIWSLIQGFVRGEARA
                     KWLGIWAGAALALILMNPARQLFDFSWATVPLLALAAMKLVELFPSFSSDNRIVFIAE
                     TVLVVALVVFSFMNAVNLVNNPLQSPEEYRNRAIGAVLPLFLLLGMTVLLAWGWSGAS
                     TREGFIAGVLLLAGFFTLANSIKAASLGSRPEIEIRRDLAAVVGSEELLRTIQDISRA
                     RTGIENRIDVLIVDLQSPSLEWALRSFEKAETAAVFDPTASPSLVITDVNQQITAGAS
                     YRGQKIIWQEEPDMKNMTIIDWLKWSFFRIAPLENREIIIWARNDLFPGGNIP"
     gene            37734..38441
                     /gene="cmk"
                     /locus_tag="P5516_00155"
     CDS             37734..38441
                     /gene="cmk"
                     /locus_tag="P5516_00155"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HRT90883.1"
                     /translation="MSTRGAAMRKQQKPLVIAIDGPAASGKSTLAEKLAQRLGFFYFD
                     TGVMYRAATLAAMKDLGGVEDEKAVTRLTAAVQIDVRPPSVEDGRVMDVLLNGEDVTW
                     EIRTPEVDRNVSPVSAYEGVRTILTNRQRDIGMRGNMVMVGRDIGTVVFPDARYKIYL
                     EASPEERARRRLKEMQLRGETHSFEEVLQSIIRRDGIDSTRQHAPLKPAVDAVIIDTD
                     GKSIEEVLEETLAIVQP"
     gene            38487..39818
                     /locus_tag="P5516_00160"
     CDS             38487..39818
                     /locus_tag="P5516_00160"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF015536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA];
                     GO_function: GO:0070004 - cysteine-type exopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C69 family dipeptidase"
                     /protein_id="HRT90884.1"
                     /translation="MCDTIVALGNSTVDGNVIFGKNSDRHPNEAHEVVIFPAAQHPTG
                     TTVKCTYIEIPQVPATHQVLLAKPFWIWGAEMGSNEHGVTIGNEAVFTRTPYGREPGL
                     IGMDLLRLALERTDSAEAAMMLIIHLLEEYGQGGNCGFGHKMYYHNSFLICDPKEAWV
                     LETSGKEWAAEKVRDVRSISNAITIGEKWDLCSANLVSSAIKRGWCRSESEFHFARCY
                     SEPIYTTFAAGRSRQACTADSLRAQKGNITATSMMRVLRAHTREIKPTWSPDRGITGA
                     DVCMHAGWGPIRENQTAGSMVSVLRSGGRSEHWVTATAVPCTSLFKPVWLDAGVPSTG
                     NAPTGEYDHAALYWRHEALQREIARDYQARIAVIKDAQATFENELTAEVARADNATAQ
                     QRRLISENAFRRADEIEEDWYAQIQGMKITRQNTLLYRSAWNKFNAQAKMK"
     gene            39838..43113
                     /locus_tag="P5516_00165"
     CDS             39838..43113
                     /locus_tag="P5516_00165"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT90885.1"
                     /translation="MIRIHLFNAGPFFDTPYQLVGWIGWFVMAALILWVARKDLTRQR
                     TSLIWKYFFALGIMAIVSTLFFGFELPPYSKVPLPNIPGEIQPPAVMVLMMVPIMLAA
                     GILGTWPAAVLGFIAGIFSALWNTHSVFTPLEISVLAVVISLALRQNYRTRFFAFLRR
                     PIGAALLAAFFSIPLFLVSTFFGTNGNLAAKLDYAFTRSWVLIGVSTIQLLIAALICE
                     VFRAPNSRYWVKFGSFEPSPVETGLQSRILYTALPLVWILLTTLVIADWFVAGRAARD
                     ILVKQLQDTSKIAVENIPPILETGQSLTAALATQKLPLGDTQQLRDLLQSKVREFPFF
                     SQIYIFNLTGSPLTGYPDDEVGEFRLSSEEELGIQLALNGVQMQNYVIHPYPGAESAV
                     ISYIAAIPDEYGLAQGVVITRTDLTMNLFSQPALIALQNLVEEGGQGVIIDETNRVLY
                     HTNPKLIMTTYTGQIPAVSGYFEDPGLTGTRYMNYASVDDLNGWKVILSVPANHSQQL
                     ALRIAIPLLVISLIISVLAYLLLRYLIKTLTQSLVLLANKADEISKGELDNRVEVKGV
                     DEIGRLSSAFEQMRLSLKLRLEELDALLKVSQGIASDLSLESTSAHLLSALRSYGADA
                     ASLVLTRGTKEGWDEGLEAYRSGEQAEEYAYLDHLLLEHLQEESLLIIPSRARIRRMA
                     LPKTAPIPSALAAIALNKSGENPGFIWVVYTQSHRFLEPEIRFLDTLAGQALLAVSNS
                     LLYQKAEVGKKRLESVLSSTPEPVLVAGEDGRLMMVNQAAEEMTLLIQLNAQGTESRG
                     EIISRLLKEFITGTNPTESRAEEMELEDHHIYLVSVSPVAVESKHLGKVCVLRDVTDY
                     KQLEKMKSEYVSTVSHDLKAPLVLIRGYASMLPMVGDLNEQQRDYSQKILDGIDDISR
                     MADNLLDMRRIDSGIQLQVEKVSPSDLLDEVIEEMQPQIKHRKIQILRELTLAQNLTI
                     EADRVLLQRALHNLLDNAVKFSSLGSQVNLRLQVNENSVVFEIQDHGSGIAPLDQPHV
                     FESIRRNTDGSPKKEWGVGLSIVRSIAERHQGRVWLESQLGKGSTFYLEIPLQHQAAE
                     TNK"
     gene            43259..47503
                     /gene="rpoC"
                     /locus_tag="P5516_00170"
     CDS             43259..47503
                     /gene="rpoC"
                     /locus_tag="P5516_00170"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HRT90886.1"
                     /translation="METKSLVALRINLASPETIRSWSYGEVLKPETINYRRLRPEKDG
                     LFCEAIFGPSRDYQCYCGKYKNPRYKGIVCDKCGVEVTRSSVRRERMGHIDLATPVAH
                     IWYTRRIPSYLGLLLDISRRNLDRVLYFAQYIVTFVDDDARQKALRRLMDEINDSELE
                     QANRINAQILEVKLGRDKKVAELQSRLKEIESKAEEQITSKLEPVIQEGQSLERQLTE
                     LSGKAAKKKIFFETADVMIAEKGEIIAPSHIASVQKAVKERLEEIEDEYKKQLDMDRE
                     QLKMAIEQVKAEAEVSMDELRNQLEDQSTESHDLSGRMRDELEELRPFTFLTESRYRE
                     LKSRWGQVFRADMGAEAFYDILRRIDLDKLSEDLWTEVRTTKSKQKRKKATTRLKVIE
                     SFRRSGNRPEWMILTVLPVIPPDLRPMVPLDGGRFATSDMNDLYRRVINRNNRLKRLL
                     ELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRRELKSLSDMLKGKKGRFRRN
                     LLGKRVDYSGRSVIVVGPKLKLYQCGLPKTMALELFRPFVVARLVQHSYAANVKSARR
                     LIERNRPEVYEVLEEVIKERPVMLNRAPTLHRLGIQAFEPILIEGSAIQLHPLVTTAF
                     NADFDGDQMAVHVPLSDKAVQEARNLMLSSKNLLKPADGEPIISPAKDMVLGIYYLTV
                     QDNRKHKGDGRVFADMDEVELAYNLGQVDLHANIKIRMNTWFNAENKRMPKAETRMID
                     TTVGRVLFNRILPEEVQFINFVLEKSGVKDLIADVYDLCGESVTTEVADAIKDIGFEY
                     AMKSGTTIAVADITIPEAKYEIIGSAQQEIDRINRDYRRGLLKEEERDKLVIDIWKKT
                     TEDVGAAVRAGMDPNGNLSVMASSGATKGGFGPISQLAGMRGLMADPAGRIIRLPIQS
                     NFREGLTALEYFISTHGARKGLADTALRTADAGYLTRRLVDIAQDIIINEEDCGTESG
                     IWIRGADDVAGQKLKERLYGRTVAGRIVNPKTGEIIAERGDILDHDLIRKVTDAGVED
                     VYVRSPLTCELIHGICAKCYGMDLGRGSLVTLGSAVGTVAAQSIGEPGTQLTLRTFHL
                     GGVASAGDITTGLPRVEELFEARRAPKGEAVITAISGIAKVLQADRVGDQKLVRVEHS
                     ELVSDEYEIPTDWEILANDASEIVTGDPVARMGDALINAQHDGRVRVEGRKVIVSYEQ
                     KESEDYEIPGTSRMIIRDGDQVEAGQALTEGSINPHTILKIKGREASQMYLLNEIQKV
                     YRAQGQNINDKHFEVIIRKMIGKVQVIRPGDTNYLPMDVVDRLEIKRVNEEMIAQGKQ
                     PARYAEILLGVTKASLSTDSFLSAASFQHTIKVLAGAAIASSEDPLFGLKENVIIGKL
                     IPAGTGFVYGRFIPAVEGETAEVEVAAEPETETKIE"
     gene            47650..50076
                     /locus_tag="P5516_00175"
     CDS             47650..50076
                     /locus_tag="P5516_00175"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase 4 subunit A"
                     /protein_id="HRT90887.1"
                     /translation="MDMGSVRKIDIDHEMQQSYLDYAMSVIVSRALPDARDGLKPVQR
                     RLLYAMYDMGVRADSAYKKSARIVGEVLGKYHPHGDQAVYEAMARLAQDFSMRCLLVD
                     GQGNFGSVDGDPPAAMRYTEARLTSIAMELLSQIDRDTVDFVRNFDDSLDEPLVLPSA
                     IPNMLVNGASGIAVGMATSIPPHNLVEVIDGLAFLLDNWQKMEDITVTDLMKFIKGPD
                     FPTGGIILQEHEQDEILAAYATGKGRITLRGRLNLEEMSRGKSRIIISELPYMTNKSA
                     LIERIAELTREGLLEGISDLRDESDRQGMRIVIELNKAADAEAVLRELYKRTPLQVTF
                     SINLLALVNNEPRLLPLKQALRVYLEHRIEVVKRRSEFDLRKAQERIHILEGLRIALQ
                     NLDEVIAIIKKSTDTDDARERLIKKYKLTLIQANAILDMPLKRLAQLERKKIEQEYRE
                     LVQLIKELLALLRSPAKQRQVVGDELKEIRTKYADRRKTQIVNLKKGKAAADLLTVKD
                     VTPVESVWVVMDEQGRIGKINAEKPPRMSGRDAPVFAMRTDSHQTLYLVGQDGQAAAV
                     SVQSVPEIVNPGEGSHYSSISPLRTTNLPASVFCLPAADLQELFVLTITRQGLIKKTL
                     ATELKGPSSDLFTLVKVNEGDELADVLLVTEKAHYLVVTRSGMAIRFEGSEVRPMGLL
                     AAGVNAIKLSAGDEVAGMIEIARKGDVVLVASNGAGWRVEPDGFPLQGRYGQGVIGCR
                     MADGVGLIGVVFGKKNFQYTLHLKKAAAKTCRVDDIGYTKRAGKSEQILQLASGDRVE
                     RIVRMMDSAE"
     gene            complement(50093..51199)
                     /locus_tag="P5516_00180"
     CDS             complement(50093..51199)
                     /locus_tag="P5516_00180"
                     /inference="COORDINATES: protein motif:HMM:NF024092.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HRT90888.1"
                     /translation="MTLTGKGMMIWKIPSCEAGNPSAIARVAASSGFSHVLIKIANDT
                     RPYNVDSSGRDLIPPVVDALRAKGIQVWGWHYVYGYDPVGEANIAVSQTRKYALDGYV
                     IDAEVEYKQAGREAVAKIFMTQLRKGIPSTPVALSSFRWPTYHRSFPYSAFLEKCDLN
                     MPQVYWMQAHNPDYDLKRSVNEFKAISPFRPIVPTGPAYGESGWYPSPSEVKLFLDTA
                     RSLGLTAANFYSWDYSRVHLVPVWDEIARYSWPAQGSVPPPSSDPVTRLFDALNAMQY
                     DPMLEIYAPNAVLVTPQQTIQGNANIRGFFGQMLTNQLNDGDFRVTSKEVKDNVIHYT
                     WTCHSRRGIVNNGKDTVGLKDGRVLYHYCYYSIE"
     gene            complement(51215..51799)
                     /locus_tag="P5516_00185"
     CDS             complement(51215..51799)
                     /locus_tag="P5516_00185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HRT90889.1"
                     /translation="MNREEALKLVRQYVKNENLVHHMLAVEAAMCFYAEKLGQPVETW
                     SVTGLLHDFDWEIHPSAEQHPLEGAPILRQHGVAEEVIQAILAHGQNLNIPRTSLLAK
                     GLYACDEITGLITAVALVRPSRSLYDLEASSVKKKWKDKAFAAGANREEIARGAEEFG
                     VPLWEHCENVIIAMRAIATQIGLAGNLEPGSSPG"
     gene            complement(51804..52451)
                     /locus_tag="P5516_00190"
     CDS             complement(51804..52451)
                     /locus_tag="P5516_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HRT90890.1"
                     /translation="MLLKLVLFSILLLLVLFGLPKVVTSLSAAGRIYSVADAPSAPVA
                     VVFGAGLQKDGTPSPVLKDRVTAAVQLYQAGKVKKLLMSGDNRFVDYNEPASMRDYAL
                     SQGVPEEAIVLDFAGRRTYDTCYRARDIFGLDDILVVTQKFHLPRAIFTCSGMGMKVN
                     GVIADLRDYHDHAERFWRIREVPATLVALWQVWVSKPLPVLGKPEPIFAEQSDNH"
     gene            complement(52536..53357)
                     /locus_tag="P5516_00195"
     CDS             complement(52536..53357)
                     /locus_tag="P5516_00195"
                     /inference="COORDINATES: protein motif:HMM:NF015084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HRT90891.1"
                     /translation="MLREWRLILSPPASGAWNMGLDEAILFSTAQKNSPPTLRMYAWQ
                     PATLSLGFAQPHLDVDLAALEEEQWGLVRRPTGGRAILHVDELTYAVAGSDDEPFLAG
                     GLLESYCKISRALLTGLARMNVTAFGDKAYTNEPPDNKKNPVCFETPSNYEITVDGKK
                     LIGSAQSRKYGGILQHGSIPIKGDLARITRVLQFPSEQARQTAANRLIARASTLEAVM
                     GTAPSMETVAEAVITGFSKAGNIHFSSSQPSEQELKMAAELVSAKYGSAQWTFRI"
     gene            53698..54930
                     /locus_tag="P5516_00200"
     CDS             53698..54930
                     /locus_tag="P5516_00200"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRT90892.1"
                     /translation="MTKKLFPLFSVLLVAVMLLAACGPKEAGPVTINLWTKEGEADGG
                     LQYVQALADAYTAAHPNVTFVVTNKEVETLREDFQTASLANAAPELLWTVSDHLGPFT
                     TADLIQPVDKLVDLGKYVGSATDAVKTPDGKTWGVPIANGNHLMLLFNKSLMPVAPAN
                     TDELLKMGADFVAANPGKYALAFNETEAFWFVPWLGGFKGKVFAADGVTPTLNTPEMI
                     ETFKFLAELKAANIMPAESNYDVMDAAFKEGTAAMIINGDWSLGGYKEALGENLGVAP
                     IPKVVATDTYPAPYTSGVFFMVPKGLEGEKLDAVVDFMKFATNKENQVEMVKKLTRLP
                     ALTAALSDNVITEDPILAGSAAQMSYGTPMPTNVEMRCVWDAIKPELLPTYAGTTSPE
                     TAAANAQKAAETCIAGLE"
     gene            55042..56049
                     /locus_tag="P5516_00205"
     CDS             55042..56049
                     /locus_tag="P5516_00205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRT90893.1"
                     /translation="MITSEVILKSLGEVGTTVLLILIGTVLVEALLYLILMKWLKYKY
                     ALPFMLLAPAAIGLIALVIYPLIWELRLSFTNMSLKAFRDPAFIGIKNYVRVFTEPVL
                     KQKMFFPLLGQTILWTVINITFHVGGGLGLAMLLNRKMRGKGIYRAIIILPWAVPQVI
                     AVLAWRGEFNFAYGYFNIILTKLGLPGVQWLADPVANFAAMCMTNIWLGIPFMMVTLL
                     GGLQSISSEFYEAAEMDGANAWQQFRNITLPLIQPVLTPAILLGTFWTFNNFNVPFFI
                     NQNELESSDILVTGLFRAAFQYNRYGFSAAFAFVIFALLLLFTIFYMRYTRSLKGAYE
                     S"
     gene            56046..56927
                     /locus_tag="P5516_00210"
     CDS             56046..56927
                     /locus_tag="P5516_00210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRT90894.1"
                     /translation="MSAKHTTPKKSFFRSGREDSPFKRILIHLVLIISCVIAIFPILR
                     ILAVSLRPGNRLMSTDLAIIPPDATLESYRIILFEKPFLSWVWNSLAITISTALIGVI
                     LAATAAYAFSRWKFPGRSVGLVFLLATQMIPASMLMIPIYILAIKLGMVGTYRGLVIA
                     YCVTSIPFSVWILKGYYDTIPVDLEEAARIDGCSQLQAFLRVLLPLSTPALAITFLFN
                     FMAAWNEYLLARVMLGSQESLLTWPLGLQRLQGQFQTQWGQFSAGSILVMVPVMVLFL
                     YSSKYLISGLTLGGVKG"
     gene            57010..60063
                     /locus_tag="P5516_00215"
     CDS             57010..60063
                     /locus_tag="P5516_00215"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucodextranase DOMON-like domain-containing
                     protein"
                     /protein_id="HRT90895.1"
                     /translation="MKKTFKYLIFILVLGLILSSCTSPTGDTRKGIGRKDVLYLNLTW
                     HQHQPLYYKDDQGVYTRPWVRVHATKDYYDMAAMIKPYPNVRVTINLTPVLMKQLDDF
                     TQNGAKDYYWVLAEKPAAELTDADKTFLLSRFFDANWDNIIYRFPRYKELLNKRGGTS
                     AEAIASAVSQFSEQDFRDLQVWFNLAWIDPEFLAQSPLKELVEKERGFSEADKAVLFN
                     EVRKIMEMVIPVHKELQDSGQIQVITTPYAHPILPLIYNSNIASVGNPAAELPARFSW
                     PNDAIVQLQKSVDIYQQHFGRAPIGLWPGEGAVSEDIIPLVADAGYQWMQTGEVVLAQ
                     SLGLGQFTRDSAETVQEADELYRPYYVTTKDGRRVAIFFRDLVLSDKLGFTYSQTPGE
                     QAAADLMQRLENIRLQLKKQKAQGPHIVSIVLDGENAWEYYPNDGKDFLNAMYRMLNE
                     SETIKMVTPTEYLELFPKQRELDTLFPGAWFSPNYDTWIGEAEETQAWNYLGKTREFL
                     AKYDVGKTKQASPEAIATAQDYMYLAEGSDWFWWYGADQDSGVDEYFDTGFRALLAKV
                     YESLGEPVPAFVRVPIIPRRPEQPVQAFSGMMAVVVDGKTDQKEWATAAAYPANGEDG
                     LEMLYYTMDAQNIYFRLDFDKEREEPESVGIYMSIPSAAANDPFALTEDAPDSLLLGI
                     PASHLFECNAGLVSGYEVFAGKWQLNPELKATAEMGEGLLELAIPLTSLGELSAGDDI
                     RVVIVVQPNSQVLPLSGPAQIVLPDLGLSDLVLEINDPEGDDHGPGTYTYPNDGVFSK
                     QNFDIESFSVATDAKNVIFTFKFYGPVPNPWGSGINLSLQTMDVYIDQDPGKGTGNRM
                     LLPGRNASLTARNGWEYVIWAEGWNYGIYAPDPATGEPKQQNSSYKILVDPARQTVTL
                     RVPKEALGDGNPAEWGYAAVVLSQDGYPSTGVWRVRDVQQTAAQWKLGGAPDDTNHTR
                     IIDLAWPAEGKGTQEEFLSTYISSKAAIETLSEKDMPQIPILLVK"
     gene            60207..61721
                     /locus_tag="P5516_00220"
     CDS             60207..61721
                     /locus_tag="P5516_00220"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HRT90896.1"
                     /translation="MTIQTPDWLKDAVFYQIFPDRFAKSERVKREGLKLEPWDTPPNP
                     YGFKGGDLYGVIEKLDYLVDLGVNAIYFNPINASASNHRYHTYDYFKIDPLLGGDEAF
                     RDLLHAAHSRGMHIVPDGVFNHASRGFWQFHHVLENGEGSPYVDWFYFDPERLKRHRP
                     WGAYPDEREQQQLNNGVGSFDAIGYTAWWNLPALPKFNTQTQAVREFIFEVAEYWLRF
                     GADGWRLDVPGEINDDSFWQEFRRRVKAVNPDAYIVGELWLESQRWLKGDQFDAVMNY
                     LVSAALMGWLIGDKMPEFAYRIGGLQRVMRPFSAAEFGDRIEYLLNLYDPEINYAQLN
                     LLDSHDTPRFITTASGDESALRLAYLFLFTFIGAPCIYYGDEVGLDGGPDPECRKSFP
                     WDESRWNQGLHAYMKDLIHLRNAHPALRRGSYKRIFSDNEALAFIRQYMGETYLVVIN
                     AGSKERQLHLDLTQTGVSSRAVESLFGLGRATLEGGSLCIEHLPSRQGVVYRLE"
     gene            61940..63631
                     /locus_tag="P5516_00225"
     CDS             61940..63631
                     /locus_tag="P5516_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRT90897.1"
                     /translation="MKVNSPRPTIEIVLPDGRVYAGNRGASLCELLQMLPEWDNPPIM
                     GAVVNGELKELTATLEMDARVRLVTMKDDDGARIYRRSITFLLEAAFEELFPKSLMAL
                     DHSVSAGGYYCQVSKGNQLGSEDLSRLELRMRELVEADLPFERQLVPLDEAISYFESK
                     GLKDKVQLLKYRQKEYLVLYRLSEHRDYHHGYMVPSTGYLKWFALQQMGEGFVLRFPR
                     RESPKQLSPMPSYPKLLSTFRQYGNWLQRLGIGSVGELNASIDAGNIREIILVSEALH
                     NLQIIDISQSIADKSEEARIILIAGPSSSGKTTFSKRLSIQLLAQGFSPFPIEMDNYF
                     VDREKTPKDEFGQYNFEALGALDTHLLSDHLKRLIQGEKVRLPKYDFHTGKRQQGEEI
                     QLRHDQMIILEGIHGLNPQLLPEIDPSSTYKIYVSCLTQLNLDRYNRISTTDTRLLRR
                     IVRDARERGYTAQETISRWESVQHGEKEYIFPYQENADKLFNSALVYELSALRLHAEP
                     LLRQVPYGTKEYVEAKRLLAFLEWFLPIDSRMIPDNSLMLEFLGGSILKDFILWK"
     gene            complement(63674..64903)
                     /locus_tag="P5516_00230"
     CDS             complement(63674..64903)
                     /locus_tag="P5516_00230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HRT90898.1"
                     /translation="MHTKMQTPWEYRYSQRTQRMKASAIRELLKVTEQPDMISFGGGF
                     PAPDVFPVEEFKKACNYVLEHKGAEVLQYGGTDGYVPLREMILRHAINLGINVDIGNI
                     LITSGSQQALDLLGKIFINRGDRILVESPTYLGALQAWNAYGAEYITVPVDEYGMITE
                     KLEAALRIGPKFIYVLPNFQNPTGTTLSLERRKKLIHLADRYGVPIVEDDPYGQLRYE
                     GESLPAVELLDSQMRVQNGTYTGNVIYLSTFSKILAPGLRLAWVIAPHGVISKLALAK
                     QGCDLHTSTFNQIVAHEVGQHGFIDRHITVIQKVYRERRNAMLDSLEEHMPEGVKWTR
                     PHGGLFLWVTLPEQFDTTEMLPEVIQEKVAYVPGEFFHPDGSGKNTMRLNFSFCSPER
                     INEGIARLGRAFKKKIA"
     gene            complement(64936..68064)
                     /gene="gcvT"
                     /locus_tag="P5516_00235"
     CDS             complement(64936..68064)
                     /gene="gcvT"
                     /locus_tag="P5516_00235"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HRT90899.1"
                     /translation="MTDYLVRGSLSEIDPAVFNLVNYEVERQFRKLILIPSESTAPQA
                     VRDSLSSVFQNLYAEGYPDEETRLMSEKEILDYPVRLSYYRRYADPRYYKGVEYADVV
                     EALARRRCAEVFATDRVTADQLYVNVQALSGAPANNAVYHALIEPGDTILGMNLLHGG
                     HLTHGSSVNRSGKLYKVAHYNVDPETEQIDYEKLEAQAKEVMPRIIICGYSSYPWIPD
                     WSRFRAIADAVGAYLLADVSHIAGLIAGKAIPSPVGHAHVITFTTHKTLCGPRGACIL
                     TTDSALAKKIDKAVFPGEQGGPHVHVFAALATTFKIAATAEFQQLQKQIIQNCVALTA
                     QLKARGLRIPFNGTDSHLGNIDCKTIVGPDGTTLSGDMAARILDIAGIVLNRNTIPGD
                     KTALNASGIRYGTPWMTQRGLTAENMREIGNLIADLLQAITPYTIEMRKGPSPRAKID
                     FNVLEQTKIKVRNLCASAATDLPHGNSGYPHFYYLDDQPRFKGKLVSLDLKGDRVLYF
                     VNYCFSSDVEALQNGQTQPTSLITPLKKVEGSLTRVEDNLYRFSFQAENFGLAATFLR
                     ALSDGYIAFDNDLPARIPGPVVVEESELVAPMPSSGKEMANNKAWYIGITNSNGNALP
                     SFKWEEKSSNELMRTPLFEDHKRLGAKLIPFAGWEMPVWYTSVVEEHLACRNAAGLFD
                     VAHMGVFQAEGSQAALFLDSVCGNDIGSLEIGDSCYTHFLDPDANVIDDTLVYRRSAD
                     KYLVVVNASNDAKDWAWLNAVRNASVAVDNHRPWVKAFGRGVVLRNLRDPREGADMRV
                     DIALQGPKSRDILLALGCDAATQRKVKALKRTELCEAVIGGYDLIISRTGYTGEKMAF
                     ELFVHPENVSSLWNDLMKVGEPLGLKPCGLGARDSLRTEAGLPLYGHEMGGERNLGVA
                     EAGFGSYVKTYKPWFIGRESFIKKELTRKGVVVRFRFAEKGVRMAHNNDPVLDEKGRV
                     IGHVTSCAIDKDGLLTGQAFIETRYAVEGTVILIYQGAPSQAGKAPSQLNPGERVTLP
                     SRATVESRFARL"
     gene            68440..70857
                     /locus_tag="P5516_00240"
     CDS             68440..70857
                     /locus_tag="P5516_00240"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRT90900.1"
                     /translation="MITPENTINEHPELDYSLKKARKMGVILLIFFTTLWVLSLTGLN
                     LFLRWSIEQSMFETETGIQDVRWLVHLVCGVFLFIPLLALSFAVKVPRVKIMLRLWTV
                     GAAFVLLSVPPKVLYLTAQVQSNLLLCGVLLLMTLVLFLLRNKQTSGSKTLPPSGLAG
                     PVFLACAALATPWVMWGALGSVEDSLSALLLGAAFALFFINAVVVDYFDKTQPPGSLV
                     NRKDFLFDGFVLFIFLFVVVSGLSQNGSQIVLCLSIPACGWLLAALSTAGRNRKNHAR
                     LATGLVSGLMLALPLAFFDMDELALFISGTDGEVFFWANRAGWNTLTFQIAFALVVLI
                     FFKQLERVHLGRRINAMLLVLSLAEVLVLYFTAGQPGFFGDRLFVVMKEQVNPTQFNE
                     INPLLEKKLALYQALTETAVTTQEEVTTQLAKWHIDFTRYYLVNGLEVNTGVLMKSML
                     EKMDGVDRVLDSPRLRPLPQPPPRMEGEITIPPEDSLWNLQMINLPLVHEQLGVFGEG
                     IIIGQTDSGVDGSHPELAGSYRGAKGSNDTNWLDPWNHTVAPNDLSGHGTGTTAIIVG
                     ATRGIAPKAQWIACTNLARNLGNPALYLDCMQFMFAPYPQGGDPLLHGKPELGAMVVN
                     NSWGCPRIEGCDADVFKPAVEILEAAGIFMSVSAGNNGYFGCSTISDPPALYEHVFSV
                     GSVDSAGELSAFSSKGPVTSDGSGRSKPDLLAPGEQVLVAAPGGTYTYSSGTSFAAPH
                     VTGVVALMWSANPKLIGEVALTRQILQESAQPYTDGSTSCGTPQNESGAGILDAYAAV
                     KAAIAVK"
     gene            70900..71496
                     /locus_tag="P5516_00245"
     CDS             70900..71496
                     /locus_tag="P5516_00245"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRT90901.1"
                     /translation="MKKQSAAPTESLTDQNAVQLACNGNAEAFSYLYEQNVKRIYNYI
                     FYRIGAEADAEDLTARVFHRAFAHINSYVEKGVPFTAWLYRIAHNLTANWYRDNHRRR
                     EVSLDDHFDLPHLSDLPERTIEMNQEKELLLKAIRRLPADRQQLILLKFLEDLSNGQI
                     AIIMGRTEGAIKSLYHRSLISLREELKRISGVETSDFR"
     gene            71509..71751
                     /locus_tag="P5516_00250"
     CDS             71509..71751
                     /locus_tag="P5516_00250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90902.1"
                     /translation="MEHGLEQNGRPGLWGLEQKLSNLLKPVTPDPEFVDSLKMKLTRA
                     PAVLVETSRRKMGLLVVSFGLFAGAFLVWLLRKRWQ"
     gene            complement(71759..71986)
                     /locus_tag="P5516_00255"
     CDS             complement(71759..71986)
                     /locus_tag="P5516_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HRT90903.1"
                     /translation="MMYGTTWCGSTKRARRLFEDEKIDYEWIDIDQDETAAKFVESVA
                     NGFRSVPTIVFPDGAILVEPSTYQLKEKLGL"
     gene            complement(72011..72346)
                     /locus_tag="P5516_00260"
     CDS             complement(72011..72346)
                     /locus_tag="P5516_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90904.1"
                     /translation="MKISERIQKEMDMAFAARKQGNEGMARVCARRAAGIVVRDFLDQ
                     HGIEAAGLNNFEILVDESVRKSLPASVLPALQHLTMRVDENYALPTGIDLLADAQAVI
                     QTLLKEVSE"
     gene            72425..73339
                     /gene="fmt"
                     /locus_tag="P5516_00265"
     CDS             72425..73339
                     /gene="fmt"
                     /locus_tag="P5516_00265"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HRT90905.1"
                     /translation="MSEKTVFMGSPEFAASILAALGNHYDVRGVVTQPDKPAGRGKKL
                     TPPPVKTLAERLGYPVIQPEKMKDPGVFEQLHAWNPDFIVVAAFGKILRQNVLNLPRL
                     GCINVHASYLPRWRGAAPVQAAIIHGDTTTGVSIMKMDIGVDTGPILIRERVKIEPAD
                     DAASLTSRLATVGATLLLQALDGYINGNLSPVPQNENGATYAPMINKEDGLLDFSQAA
                     EMLERKIRAYSEWPGAYIRLGEEALKVRRAKVVPGSGVPGQRETCNGYPCIHTSDGKL
                     VLLEVKPAGKNWMNAADYLRGSRSWISN"
     gene            complement(73488..76064)
                     /gene="mutS"
                     /locus_tag="P5516_00270"
     CDS             complement(73488..76064)
                     /gene="mutS"
                     /locus_tag="P5516_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HRT90906.1"
                     /translation="MAEDVTPIRQQYLDIKKQYPEAIVFFRLGDFYETFDHDAQITSR
                     ELDIVLTSRNVAKGQRVPMAGIPYHAAENYLARLIDKGYHVAICEQVGEQPAKGIFPR
                     EVVRVVTPGTVIEPGLLHSNRNNYLAAARVEDGQLSVAYADITTGEFAVTTIESRDPL
                     SDLRAELTRLDPAEILVPESLKLNTAFSGHITSLPDWRFETSRCREVLLRVMQVTTLE
                     GYGLENAPSAIQTSGAILQYLEQTQPAALSMVKSISTYSTGEFMTLDAATRRNLELTE
                     TIRRGETQGSLLSILDHTLTPMGHRCMRQWVSKPLLRIDSINERLDIVAVFHQHSLVR
                     ADFRVQLQHLGDLERLANRLTGNVALPRDLAAIRAILQKLPAIRDTLSQDVLQLSHKL
                     IDFDLCPEVLNLLEQSLADEPPATLQHTGVIRVGFSEELDQVVEMSNHAREWISNLEA
                     VERERTGIKSLKVGYNKVFGYYLEITHSNTAQVPAEYIRKQTLVNAERYITPEMKEYE
                     ALVLNAEERIREIENRLFKEVCQRLLPHLSALLKSARLLGELDVLSSLAEVTAIHAYV
                     RPVLAEEPMLNIVDGRHPVVETTLQSERYVPNDAVMETGEIIRIITGPNMSGKSTFLR
                     QVALIVLMAQIGSYVPAASATIGIVDRIFTRIGAQDEIHTGQSTFMVEMIETANILNH
                     ASSRSLLILDEIGRGTSTYDGLSIAWSVIEYIHNHPRLRARTLFATHYHELTQLSDLL
                     PGIRNYNVAVAENGTQVIFLHKIVPGGTDRSYGIHVAQLAGIPRPVISRASEILQQLE
                     ASSGSTIKVDLPSSQQLTLFPETNPLLDELKAIDLNTLPPIEALNILYEWRRRFLSAE
                     ES"
     gene            complement(76135..76779)
                     /locus_tag="P5516_00275"
     CDS             complement(76135..76779)
                     /locus_tag="P5516_00275"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT90907.1"
                     /translation="MVEHLEFVKNYTDLSTDRGFQFEFTCARCESGYRTTFKPSLTGK
                     LSGALDTASSLFGGFLSQAANISDRVHSAAWERAHDENFTEAVKEIRPYFIQCPRCNA
                     WVCREKCWNKKKGLCKDCAPDLGVEMAAAQASKSVEEVWAHAAMAEEDKKLGTEYWRE
                     GITATCPNCEKPLAANAKFCPECGYALKPKDKCPHCGSKILPDAKFCGECGQKL"
     gene            76886..77821
                     /locus_tag="P5516_00280"
     CDS             76886..77821
                     /locus_tag="P5516_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRT90908.1"
                     /translation="MQRVQTCDIFTRIQLGIGTWAWGDKLVWGYGNSYSEADIREAFK
                     VCVGGGIRFFDSAEVYGQGKAETILGELCAQEDEEFVIATKMMPFPWRLFHNSLKFAL
                     QNSLRRLRVNQVQLYQMHWPLPPVKVDTWMDRMAEVVDAGMVKAVGVSNYNLEQTITA
                     HEALKKRGLRLASNQVEYHLLERRIEQNGLMEYCLEEGIKIIAYSPLAMGILTGKYTP
                     ENPPAGTRAAQYNRSYLEKIQPLIKTLTRIGNDADGKSAGQVAINWCIQKGTLPIPGV
                     KNAAQAVQNLGAIGWQLDAEEMALLDEVSLAVTLP"
     gene            complement(77834..78622)
                     /locus_tag="P5516_00285"
     CDS             complement(77834..78622)
                     /locus_tag="P5516_00285"
                     /inference="COORDINATES: protein motif:HMM:NF014654.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HRT90909.1"
                     /translation="MDFQIAGHQTEGYKINTEVYSGPLDLLLQLIEKAELDITRLSLA
                     QVTDQYLEYMHHLETQDPAEVSAFLVIAAKLVQIKSAALLPRQTIPSLQVEEDPGEAL
                     ARQLLVYKKFKDLSRFLESREEAGYHTYLRLDANPAVQIPSKLDISELTLEALVEAAR
                     EVFSNPNNLPPLSEVVASPRFTIRQKIHVILEALQKMGATTFQQLITTRSSRVEVVVT
                     FLALLELVKRHMVGADQNQLFGEISLQPEGEFSTLDDQELEFVE"
     gene            78689..79798
                     /locus_tag="P5516_00290"
     CDS             78689..79798
                     /locus_tag="P5516_00290"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRT90910.1"
                     /translation="MMKSGTINCTYCGEPYPSAGLPFRCTCGGIYDFSRFPDFTIPKA
                     QAVPRGVWHFKDSLGLPAEAPIVSLGEGNTPLMSWSLHHRQVYLKMECQNPTGSYKDR
                     GTAVLTSFLLARGVKEVVEDSSGNAGASLAAYCARAGIKAAIFIPESASGPKRWQIER
                     YGAEVHRVPGPRDNAAEAVLRAAGAGKVYASHAYMPFGLTGIATIAYEIWQSLGREPG
                     SLISPVGHGGLLYGVMRGFDALFSAGLIGKKPIYIGVQAENCAPLFQAYRQQKLNPVK
                     VEISASLAEGTHVSCPVRGAQILERMQNHQGRMVMGSESQLVALYRLSARSGYFCEPT
                     SCLSLVPLLDDKIDLVEPVVAIMTGSGYKTNIILQ"
     gene            79874..80602
                     /gene="rnc"
                     /locus_tag="P5516_00295"
     CDS             79874..80602
                     /gene="rnc"
                     /locus_tag="P5516_00295"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HRT90911.1"
                     /translation="MHNECNRETPEELAARIFPDLTDILLLSRALTHRSYLNEHPEAL
                     EDNERLEFLGDAVLDFVVGDWLYNRYPEMPEGDLTRMRSALVHTAQLADFARSIQLGR
                     AMRLGKGETQAGGCERTGLLCDTFEAVVGAIYLEMEVEGVKRFIYPMLESCADDILIN
                     HKDEDPKSKLQEWAQGNGYPAPVYTTRDVSGPDHSKFFVVDVIVNGKTCGTGSGTSKQ
                     AATKQAAADALNKLHLSDWKPTQR"
     gene            80599..84198
                     /gene="smc"
                     /locus_tag="P5516_00300"
     CDS             80599..84198
                     /gene="smc"
                     /locus_tag="P5516_00300"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HRT90912.1"
                     /translation="MKPKLRSLELHGYKTFAGRALFEFPGNITAIVGPNGSGKSNIAD
                     AVRWVLGEQSYTLLRGKKTEDMIFLGSDQRPKAGMASATITFNNEEGWLPIDYSEVAV
                     ARRAYRDGSNEYLLNRQRVRLKDTTELLAQSGLAERTYTIIGQGLVDAALSLRPEERR
                     KFFEEAAGIGLFRSRREEAITRLDATRRNLERVTDILGELEPRLRSLERQAKRVEEYD
                     QVKDDLQVLLKDWYGFHWQNTQKELLRNREVLKNQEIKVEAARTRRGEIEVKVDAARL
                     RLQELRSLLNAWHMETAELHRQREKISRDIAVLEERFRAYQNQDISLKAELSRNEDLK
                     HSNQEKLTALMDEEERINLELAQCAEQLEEIRISFDERKANRDQLETKINLARKQLLE
                     VETRQVQLKAHQRELTGRVESFEDSRKNLLQSIDQNQNGLNKTQKEMEEISAGQVKIQ
                     QSADIYVRDIGIAEEKLAELELQEKEFSGEISRLEAEHARAKAQLEVLHQAELAFSGL
                     SEGARSLLTAASQGKIPAGLRTLSSYLDVPYELETAMSAALGDQLDAVLFGEGVDPER
                     ILSYLENDESGRAMLIPLTAAGKGQARKFAGPGILGVASDLVKPSDLNRGLVNFLLGS
                     VIIVNDRATARRLLKDLPVDGRIVTLKGEVFDGSGVIIAGKENRAGIISRPREKKDLT
                     QQVNRVAEKLAEKKKELEELQKNIISLRNEKSELDKAAREEQAKLQQINATLAGRERE
                     VAKLMQQLEWQNNQINTLVSQAKSAEMDMDGIGKEIAANENAIIELNQTIRQDNEQLR
                     GIPIDEIQQQLVHWNTTQAVTERALREVAGRVNESKQTIAQNQAVIEQVNRRIEEVVT
                     ATAEVRASIEESKKAEGLVNLSLDEFSQKINPAESELRKLESEYSELQHEYSQTQQFE
                     ANADRVYTQAQLEYSRSREALEGLRRRIEEDFGLVTLEYSDEVTGPTPLPLEGVGTLV
                     VLDELPKGLEDSINRQRALLRRMGAINPEARKEYQEVKERHDFMKTQLADLQKADSDL
                     RQIITELDELMHRQFRTTFNEVAAEFKEMFSRLFGGGSARLVMTDTENPTETGIDIEA
                     RLPGRREQGLSLLSGGERSLTAVALIFSLLRVSPTPFCILDEVDAMLDEANVGRFTEL
                     LKELSQETQFIVITHNRNTVQVADVIYGVTMGRDSASQVISLRLDELSDEMVK"
     gene            complement(84250..85563)
                     /locus_tag="P5516_00305"
     CDS             complement(84250..85563)
                     /locus_tag="P5516_00305"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF025010.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRT90913.1"
                     /translation="MTKENIERTPKPASKKREARLQRENRQKKIMITVLIAIVAVVTL
                     LVGFGVLQGTVLKNQKIVAKVGDTNIKASQFAKRVQYERLSYVENFTSYAASGYAMFF
                     QSQMLQMQNALDNYIQFGSDVLEKMINEAVVAHKAAEMGISVTDEDIEKELERGFGFF
                     PDGTPTPQPTYAYLPTSTMSAQQLAIITITPTPSPEPTATSEPTATADPSLATPTVED
                     STPTSVPTATQIPPTPTAYTRDGFKQLFNQMLTSINEQFPFTEADFRAYVKSLLLNQK
                     VYEAVTKDIPREQEMVWARHILVASEEEANAVIERLKKGEDFGTIAAELSIDTSSGVN
                     GGDLGWFSRGQMVEPFETAAYALKIGEISQPVQSDFGFHVIQLLGKEVRALSDSQWET
                     LKQKAFTDFIEAAKADIKIEKKDVWASVVPNTPAIPAQYRIAPGQ"
     gene            complement(85645..85929)
                     /gene="rpsR"
                     /locus_tag="P5516_00310"
     CDS             complement(85645..85929)
                     /gene="rpsR"
                     /locus_tag="P5516_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HRT90914.1"
                     /translation="MSDEITNESERFSGQRRFVARPKICQFCADKNIAIDYKNTDLLR
                     RFVTEDGKIRPRRQTGTCARHQRSLASAVKRARHIALLPFTGERWSESIR"
     gene            complement(85941..86345)
                     /locus_tag="P5516_00315"
     CDS             complement(85941..86345)
                     /locus_tag="P5516_00315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRT90915.1"
                     /translation="MGRDLNKVMIIGHLGRDPEMRFTPSGRPVTTFTVATNRSWNTGD
                     GERHSETEWFYVVSWGNLAEICKQYLTKGQQVYVEGRLQTRRWEDSEGVKHSNVEIVA
                     SEMMILGEKKENAQPNGDLQETDFQAVDEYPF"
     gene            complement(86378..86662)
                     /gene="rpsF"
                     /locus_tag="P5516_00320"
     CDS             complement(86378..86662)
                     /gene="rpsF"
                     /locus_tag="P5516_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HRT90916.1"
                     /translation="MRTYELVLIFHPELDENALNEALDKVKGWITAGGGSISKVDNWG
                     KKRLAYLIRKQREGQYFLLTAEMDPALTSELDRNLRFHEPVIRYMITVVA"
     gene            87037..87110
                     /locus_tag="P5516_00325"
     tRNA            87037..87110
                     /locus_tag="P5516_00325"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:87069..87071,aa:Gln,seq:ctg)
     gene            complement(87234..88412)
                     /locus_tag="P5516_00330"
     CDS             complement(87234..88412)
                     /locus_tag="P5516_00330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT90917.1"
                     /translation="MTDPIRKAVFITSVDYESPIQVGDHQLARQFASNGWQVIFISKP
                     ITPWHLVARDNNSFKRRFSTCLSNGRWYTHGKGIICSFVPFAAVIPKNEIFLAKKWVF
                     QYWHKTIFPNIKKNLAVKGFLDVDLLHIRDPLQGYLLDLINAKKKVFRVADNDSGFSS
                     YNPFYTQAEHKIASKVDLVAYSAHQLENRVRQLQPKQAVFLPNGVDFEHFQKVKAIPE
                     LYRSCSRPLIVYAGSIDFWFDFDLINRLAIALPKFTFVIIGPNQNFGSRFIKVKNLIL
                     AGPVPHSELPAYLSYADIGIIPFNQRDFPTLVNAICPVKLYEYMACGLPVVASKWDEL
                     EHIASPALLCSSYEEFLTALMNYQPIKERADTYIQFARQHDWSRRYNALVKLISDVGP
                     "
     gene            89251..89688
                     /locus_tag="P5516_00335"
     CDS             89251..89688
                     /locus_tag="P5516_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90918.1"
                     /translation="MKKLAIALILLLATPACSISNQHSVEIQPGLDVSTPAKEVPPPA
                     PQPLPPAQLSGDLVSPDDFEYLGAFRLPGGEDRPQTFAYGGNAMTYNPDGNSLFIMGH
                     DRMPYGDLPDGNQVAEISIPKPVISKNIEDLNTADLSKNFQMC"
     gene            89682..90692
                     /locus_tag="P5516_00340"
     CDS             89682..90692
                     /locus_tag="P5516_00340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90919.1"
                     /translation="MLKRQFSEYDEIPKAGMQYLSRAETGAKIHVAFGEHLQSEQIPT
                     HGWFSPTLSIPDFQGTWFIGNQDLYSVNGYMFEIPAEWADTCTGGRPLGTGRMRDGGQ
                     GGMGPTLFAYRPWNADGSPLPAGTRLDEVTLLLYENAYNTEEIVRSMDGYQHPDEWGG
                     GAWLTTSGGKAAVLFAGTKSNGEKYWYGYIHPDGANLACVDAEVTDFPTCRMADGSVC
                     PQEDYTACCDAVAGECVSNRGWWTTRFDAEFILYDPADLAKVATGQLEPWQPQPYAVI
                     DIDEHLFLSPPEWDLVELGWSDQRRSRIGDVAFDRQNGLLYVLELYGDGTKPVVHLWK
                     VH"
     gene            complement(90694..90963)
                     /locus_tag="P5516_00345"
     CDS             complement(90694..90963)
                     /locus_tag="P5516_00345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90920.1"
                     /translation="MLLDLKELFPPDQRTIIEPHSSSLQFLTIWDLIVISLGIVAGRS
                     HRRLSTPGHQGFPSLPGGMALSNRKSATSFFNLELNFPLQGKYAI"
     gene            91377..92777
                     /locus_tag="P5516_00350"
     CDS             91377..92777
                     /locus_tag="P5516_00350"
                     /inference="COORDINATES: protein motif:HMM:TIGR00797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HRT90921.1"
                     /translation="MALKQIRSFFRDPEYYRGLLRLMLPIALQNLITSSLNFVSVVMI
                     GQLGEVSIASVGLGNQVWFLLNLMVFGISSGSAMFSAQLWGKKDVHNIRRVVGLTVKL
                     GLVAAAIFFILARFFPGAALRVYSNDPEVIATGSRYLRIVCWTYGFYALSAVFSMASR
                     AVGNVRLPVAVSTSALIINVLIAYPLIFGVRFLGIPALGVEGAAIAGVIARVIECGAL
                     LFFIYRNRENPVAAGPRYLMELDWEFIKRVMVPVLPVIGNEILWSFGITTYNAIYGHI
                     GTKAVAAINIISTVDQVAFVVFLGLGNATAVLVGNLIGQGRMEKAYLYAGRSLILQVL
                     GAVVMGLLVILFAPLILGFYRVDPEVIVNARRLLWILGLGLGVRAANHMIVIGILRSG
                     GDTRFSLVLDGLVIWLVGVPFTAAGAFLFHLPVYLVYALTLTEEITKASLGLKRYLSK
                     KWINDLTGRVSEIIPE"
     gene            complement(92764..94095)
                     /locus_tag="P5516_00355"
     CDS             complement(92764..94095)
                     /locus_tag="P5516_00355"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT90922.1"
                     /translation="MIEKSNPGQPGVYLNLPEMSDSQQSKNLKHNFTVNLLDGGLFGF
                     GLGFASFTTMIPLFVATLTSSATLIGLVPAIHNVGWQLPQLLMAKRIARMERVKPYVT
                     FMTVQERLPILGLGIVGLLVPRIGVRVALILTFILLIWQGLGAGLTANAWQIMVSKII
                     PSNILATFFGAQSAAANLLASLGAYLAGTLLVVLNPPYDFSTCFFIASLLLVSSWVFL
                     NLTREEKREMPSGGCVPQPLWHDIRSILKRDRSFTHFLISRFIAHFGMMAFAFYSVYA
                     VKILRMTTVQVGVMTSILMVTMMVMNPLLGRLSDRWSRKWVLVIGSVCAAVSALLAVL
                     IKEVNLFALVFLLNGAANTAFWTIGITMSLEFGEEAEKPTYVGMANTLVAPAAIIAPL
                     IGGLLADAFDYVTAFITSAVMAFIASGILILLVNDPKKPEPYEQPAGFTQG"
     gene            complement(94107..94340)
                     /locus_tag="P5516_00360"
     CDS             complement(94107..94340)
                     /locus_tag="P5516_00360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90923.1"
                     /translation="MPRIKCHYLDCSFLDEGICTAGMVELDPDTGCKTYTVSAEALST
                     EDWDAEEDELEEWEDMEEEGEDDWDELDYDDED"
     gene            complement(94342..94806)
                     /locus_tag="P5516_00365"
     CDS             complement(94342..94806)
                     /locus_tag="P5516_00365"
                     /inference="COORDINATES: protein motif:HMM:NF019499.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HRT90924.1"
                     /translation="MKSSRPRTSTLIIQISAIIGLLDSIYLAYVKIAKTPIYCTPGLG
                     HCDVVNASRWSILFGIPLGFYGVAGFGLIFLLAAFGLHQRANHPYIDLILFGFSLAGF
                     LFSLYLTSIEIFVLKTVCQWCLLSAMMMSVIFITTIIRLVVKSPSTTASGGK"
     gene            complement(94913..97162)
                     /locus_tag="P5516_00370"
     CDS             complement(94913..97162)
                     /locus_tag="P5516_00370"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HRT90925.1"
                     /translation="MVSTTKTSPLLENLLDILPDNYSRVDREFIIQAYNFAENAHAEQ
                     KRASGEPYITHCVAVSIILAEMHVQPVVVAAGLLHDTVEDTSVTLQDIQRNFGDEVAK
                     LVDGVTKLTHLPRVSRADQHDAEIFGKNMEKNQEEQEMLLNSQHARDRRRDLANETLR
                     KTFLAMGEDIRVILIKLADRLHNMRTLGFVPEAKRRRIAQETLDIFAPLANRLGIWQL
                     KWELEDLGFRYTNPEKYKEIAQNLAETRLERERQMKAIIDRMAKLINEAGIKVEISGR
                     SKHIYSIYRKMVEKDKPFELVRDLRGVRLIVPDIPSCYAVLGIIHTHWRPVPNEFDDY
                     IAAPKDNFYQSLHTAVLYDDGKPLEVQIRTPEMHENAEYGIAAHWRYKEKGPRNEQYE
                     QRINWLKKLMEWRQEVEDANEFVEGMRTDVFQDRVYVFTPRGDIIDLPAGATPIDFAY
                     TVHTEIGNRCRGAKVNGKLVSLDYTLHTGDQVEILAAKQGGPSRDWLNPTLGLVKTQR
                     ARSKIRAWFKHQNREQNLAQGKEILERELRRLGLQQYDLEKLSRGFDYRTLDDMYVAV
                     GCGDMSLGHIINTLSEGEADESRLFEIKSSTQAKDISENAVSVLGLRNILTTFARCCN
                     PVPGDSIVGYITRGRGATIHRQDCPNILRMTDRERIVRVAWGEPQHTFPVEIEIKAYD
                     RQGLMVDISSVLSNENINLLDIKINVSHNLASIVMKLEIADIVQLSRVLTLIENLPNV
                     MEAHRLNPG"
     gene            complement(97313..98473)
                     /locus_tag="P5516_00375"
     CDS             complement(97313..98473)
                     /locus_tag="P5516_00375"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRT90926.1"
                     /translation="MNPQNTYDVIIIGAGSLGTPAAFYFASAGLKVLVLETVPSVGQG
                     SNKRAIGGIRATHSDPAKIRLCLRSIEIFSTFKEVYGKDIEWDQCGYSFIAYTPREEK
                     ILKDLLTIQHTYGLVNNWYAKDDLLKIIPDLTPQGLLGGTFSPNDGSASPLLSNHAYF
                     TLAVEKGVTFHFCEPVTGMVVKRGKVISVTTSRETYSAGVVLNTAGASAAEIGRMAGV
                     EIPVRADSHEAAITEPVARFLGPMIVDIRPAPGSSNYYFYQHTTGQVIFCITPSPNIW
                     GEDVNETSSFLPMVARRMISIMPRLKNIRVRRTWRGLYPMTPDGFPIVGWSSQVSNLL
                     LGVGLCGQGFMLGPALGELLTRAVTGDVLPEDADTLRYLSPQREFEGQELLK"
     gene            complement(98470..100563)
                     /locus_tag="P5516_00380"
     CDS             complement(98470..100563)
                     /locus_tag="P5516_00380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT90927.1"
                     /translation="MTGYRIKQHPILPIPEPEMIEFFWQDKPLSAHKGETISAALFAN
                     GIRTFGHHHKDGSPLGIFCANGQCAQCMVIADGKPLKSCMEIVTNGMRVYPNDGHPRL
                     PEVSVVPELKPIPEIDIPVLIIGGGPAGLSAAIELGKLGAEVILVDDKHRLGGKLVLQ
                     THRFFGSSKAVYAGTRGIDIATRLENEVRAYPSVQIWPQTTCLAVFSDQKVGVLKDGK
                     HYVLIRPQVLLVATGAREKFLAFKGNTLPGVFGAGAFQTLVNRDLALPAEKLFIVGGG
                     NVGLIAGYHALQAGIGVVGLVEALPQCGGYKVHKDKLVRMGVPIYTSHTILSANGQDH
                     VESVTIARVDEQFQPVPGSERSYACDSVLIAVGLDPVNEFFHKANDFGIPAFVAGDAE
                     EIAEASAAIFSGKIKGLEVAHHLGLNTGEIPAEWYRMGEILKSKPGNVLSEKMPDLPE
                     GVLPVFHCSQEIPCDPCSTLCPQHLIFIDQQDIRSTPKFVQDNFACKACLKCIAGCPG
                     LAITLVDYRSSSERPLVSIPYEFSRESIHIGDQVTLEDTQGDELGTAEVVNIRALKAN
                     DRTIVIQVEVEQNIAPHIAGIRMQQPAITQPMLQYVNHVTDDTIVCRCEHVTAGEIRE
                     CIRNGYRDINEIKTVTRACMGACGAKTCAPLIHRIFREEGVPYDQVTDPSRRPLFVEV
                     GLGVFAGENGEGDNQ"
     gene            100887..102545
                     /locus_tag="P5516_00385"
     CDS             100887..102545
                     /locus_tag="P5516_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015390.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008810 - cellulase activity [Evidence
                     IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate binding domain-containing protein"
                     /protein_id="HRT90928.1"
                     /translation="MKRILFSLIAALFLLGAAPQPAPTMQADNPNPPTQTVKLIFIHH
                     STGENWLRDDNGGLGMALAQNNYFVSDTNYGWGPDSIGDRTDIPNWTEWFASENTPTI
                     MQAVYNESRQNSEYTRNRPDPGGENTVILFKSCFPNSALEGNPNDPPSEEGDLTVGHA
                     KWVYNQILPYFKSQPDKLFIVITAPPLSDNTYAQNARDFNNWLVNDWLRENNYTLNNV
                     AVYDFYNVLSAPDNHHWYNNGRVEDVINKQRNTNAYPSGDDHPNQRGNRKASEEFVPV
                     LNVFYNRWRASAPAQPPVQEEVQAHTGQDGQSPLQPGSQPEGGGLIDAFEGGTPPGTT
                     GWETYRDETTPSIITCAPDSSMAHSGNASMEMEFFVPADSWVTCMLYNEQPQNWSGQQ
                     GISFFIHAQQAGTVIHVNLYAGDPANQESHVYVLETPPDSGSGWALIEIPWGAFHRVE
                     WEENSGVPFTLTNAVLAIGFGASANPDENNKGTVWIDDLGFLGSQVGGQPTTAAENPQ
                     PITAQQQEQPRSGLRRSLPCGGSVALPLGLVIVSLLKRRKPFRK"
     gene            complement(102716..103456)
                     /locus_tag="P5516_00390"
     CDS             complement(102716..103456)
                     /locus_tag="P5516_00390"
                     /inference="COORDINATES: protein motif:HMM:NF017655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraX family protein"
                     /protein_id="HRT90929.1"
                     /translation="MKLNGFALKLIALVLMFGEHFGLYLGELLPAHVPLYLTYAGRIV
                     APIFFFLSVESYFKTSNRRRYIIRLFAWALAMQVGNLIISEIVKNAYHAEGSLGQNIF
                     LSIAIGISMIAAFEWGRKQSGIKKWGGFVFAGILAFFSLLTESSYNGLAMFIVFYFFY
                     NKKPALYFAYAGLCLLFFLWGLNNIEYFWEFEYQWMMIAALPFIILYNGERGPHSLKY
                     LFYAFYPLHIWALYIIRYSILAFYYPIQ"
     gene            complement(103640..104726)
                     /gene="prfB"
                     /locus_tag="P5516_00395"
     CDS             complement(join(103640..104659,104661..104726))
                     /gene="prfB"
                     /locus_tag="P5516_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HRT90930.1"
                     /translation="MEELIATLQANLTTVHSLLERLDLPQKENELAALTLKSQDPNLW
                     DDPRQAQIVMKNLADLRDEISTWKNLEKRISDALELAELADSSLQQELEAELTAITNQ
                     VANLEMATLLGGKYDRGNALLTINTGEGGTDAQDWAAMLERMYLRWCERRGFETEILE
                     QTEGEEAGIKTVSIAVNGAFAYGYLRAEKGVHRLVRLSPFDAAHRRHTSFAQVEVLPE
                     AVQEDPTVVINPDEIKIDIFRSSGAGGQNVQKNATAVRLTHLPTGIVVTCQNERSQMQ
                     NRENAMRVLRARLLDLAQIAADQHLSDLRGAYQKAEWGSQIRSYVLHPYQMVKDHRSG
                     YEVGNSLAVLDGDLDGFIEAYLRMKKE"
     gene            complement(104736..105653)
                     /gene="ftsY"
                     /locus_tag="P5516_00400"
     CDS             complement(104736..105653)
                     /gene="ftsY"
                     /locus_tag="P5516_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HRT90931.1"
                     /translation="MSALFNKWRESLEKTRKATFGRIITVFGGSEITRETWDELEALL
                     IQADVGVETSQRIIERVSTAIHEKGLTKTNEFIQTLEETLLDLLLPSTPLELDSHHPA
                     VVTLVGVNGSGKTTTAAKLGFIYSNEGKKVLLAAADTFRAAAIDQLQVWGDRLHLPVI
                     AGQPESDPGAVAYDALQAGIARNMDIVLIDTAGRLHTRYNLMEELKKVHRVLAKALPG
                     APHATLLVLDATTGQNALHQARAFQQAVNVSGVILSKLDTSARGGMAFAIQSELGLPI
                     YYAGLGEKPEDLQRFDRYQFVKNILGVKE"
     gene            complement(105686..>106408)
                     /gene="rlmN"
                     /locus_tag="P5516_00405"
     CDS             complement(105686..>106408)
                     /gene="rlmN"
                     /locus_tag="P5516_00405"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HRT90932.1"
                     /translation="CISSQSGCAMGCVFCATGQMGFRRNLSAGEVIEQVLFLGRELSQ
                     SGEKITNIVIMGMGEPFHNYDAVLEAIDRLNDPNGLNLGARRFTISTVGIVPGIRRFA
                     AEKRQVNLAISLHAANDTLRSVLIPANRKYPLSSLMEACGEYIRLTNRRLTFEWALIR
                     DINDSVEHARELAALLKGLLCHVNIIPLNPTTSYNGKATTEERAQEFKQVLENNGIPC
                     TIRARRGIDISAGCGQLAARRA"
BASE COUNT        25858 a        25939 c        28488 g        26123 t
ORIGIN      
        1 accgtttgct ggttggataa ggaaaaaata acaattaacc cgcttgtgat ttgtggtatg
       61 atgaaattaa tttcaggctt agtgtaacga gatgttcttg tgtatgcatt atcattacct
      121 attcaggaaa tgaagccatt catacaggat tactatgaac caaaaagaaa accctcaaag
      181 cgtgattgat aagtactcaa aacgacagaa atacatgccg tatgttcttg gcggcctcgc
      241 tggtatcctt gccctggcgg gcattttcat catcatcagc gttgccaccg gctcaggcaa
      301 tctatttaag gggatgtttg ccaccaaaac gcccacgcca accgcgacct tcacccccac
      361 cgccactgtg ccatctccca ccccttcgat gacgccgacg atcactgaga caccgacgcc
      421 cgccgcaaca gacacgccat caggtcctca gttctacaca gtcaaacagg gcgatgattg
      481 ctggacgctg gcgaatactt ttggtgttga tctgttagtg ctgctggcga tcaacaattt
      541 tccatccggc acctgccctc tcgttccagg aacggatatt atcatccctg cccccggtca
      601 gactttgcca accagcacgc cgctgccggc tgatattccc cgcggaacag aaatcgagta
      661 caccatccaa ctgggcgaaa ctttgcagga tatcgcctct cgatttaaca gcacgcttga
      721 ggatatcaag acccgcaata atattaaaaa tgaaaattta atctatgttg gtcaggttat
      781 caaggtgcgt gtcaatctgg ttaccgctac accaactgtg gcgccaacca gcacgctttc
      841 actgttcact ttacaaccca cgaaacccgc ggtgacgacg accccgtaaa gagttgctcc
      901 gcgcattgac ctaccagata caaaacgatc ctctcgaaga gacgggggga tcgttatttt
      961 ttgagttgga ttttatttct ggcccatctc gctgtgtccc gcagccaatc aagctcagcg
     1021 gtatcctcgg ttctttttat actgccgagg gcgtttttct ctcccaagtt ggccagcctg
     1081 atgatcacat tgcgcttcaa gccattgtag cgggctcgcg ccagggctgt ttcagcaaag
     1141 cgtgatttaa agtcatccat agatagctca aggaattcca gcatctcttc cacggtcatt
     1201 tgaccggcta tacctatgga actggatgat tggtaccccc gctggttcca ggggcagacc
     1261 atctggcaga tttcacaacc aaagatccag ccgccaaatt tttccatcac ctctggggga
     1321 atcatccctt tattttcaat gttgtggtaa ctgaggcagc ggttggcgtc aatcacccgt
     1381 tcgggcagga tgcagccggt tgggcaagac tcaacacagc gcatacagct gccgcaatga
     1441 tccgctgcaa agggagggtc cgcttgcaat gcgatagcgg tgaagatttc ggcgagcaga
     1501 agattggagc catgttttgg cgaaatcaaa caggaattgc gcccaatcca acccaggccg
     1561 gaccgtactg ccagttcacg ctcaagaaca ggggcgctat cggtatacac tcgcggtgcc
     1621 ggcgcttcgg gattgagcgc tgtgagatac tgcagcaaag gggaagccaa gttgggaata
     1681 aggtgatggt aatccttgcc tgaagcgtat ccactgatca agcccgttgt tttttcttca
     1741 atttccgcgg ggtgctgcag cggataacga taacctaaga gaataatcga tttcaaaccc
     1801 ggaaaaaatt tttctgggtg cagcctgttg gaaacgtgat aagcagacgc gagataggtc
     1861 atgcctgcgt tcaaaccgcg ttccaaccag ttaacataca tatggtgaaa atcgagtgtg
     1921 tctggggtgg tgataccgca aacagtaaag ccaaggctca aggccctggc tttaatttcg
     1981 ctttccagtg aagaaggtat ttgagacact gccttcgggt gcaatcctag ggggtacccc
     2041 acccggcgga tacagtgcca gtgattgtag gcgtgatggt tgcggttggc gcgttgcctg
     2101 tgggagtggg ttctccgacg atgatgctga tgttaaatgg gtctccgaag acataaccgc
     2161 tggagttata cattttccag aagctgccgt aattgccggg ggtggtaggt gcgataaaag
     2221 gcacactgat ttcagcgaaa tcaccaggct tcacctcggc tggcagtctg aacatcaact
     2281 ggctgcccgc tccatataac acaccttcca ggtaaagtac cgagtactcc tttgtccagg
     2341 ttgttttccc ggtattctga agtttccagg tttttacgat gtgttctcca ggttttacca
     2401 cgctgccatc agggtatgtg acatccgtga tgaacaaggc gttatccgcc gttgaagttt
     2461 gtggctgcgt aggaacaaga acggttaccg gcgatgatat cgcggtgagg ggagtggggg
     2521 tctgagggct gggagtggct gtggcggttg gttcaagtgt gggggtggca gttgggttgg
     2581 acgccgcagc ctgagttaat ccagcctgca cagtggcggc gacctcggtc attttctggt
     2641 tagggtcaat ggatggtttt cctttacagg agaccagaag catcccgata acgatcacaa
     2701 tgatgaagtt gaacagcatc cttcttttta acatgaataa cttcctccgc ttagttgaaa
     2761 cacgcgccaa ttgtccgata ccttgcgtat cctgtcaaga gtggaagaaa tcgcgaaaga
     2821 gataattaat tcaggaattc tgtgttttga ctaccaggag gattgccgag ggaaaaccct
     2881 tttttaccaa ggaagttgat gtaagttacc gcgccaggaa caattgtgac ctcatattta
     2941 taggctgaag cattgtaata ggtaaggatt tcgtatctgc cagcaggcag gtcatttagc
     3001 acgaaatttt cctggtaata ctgatcttgc tgagattctt cgacaatgga tccgtcatag
     3061 gtgcgaatgc gccacacggt tcccgtatca agcgaggtaa gacgtatgtt ccagttggct
     3121 aaaaggttcc ccatattatt gataattctg ccggcgagta ctccatgctc cagtggagga
     3181 accatccaca attcagggtt ctggagcagc atgccatttt tacctttcac ccgtacttca
     3241 aaatgcaggt gaggtccgct ggtgttgcca gtcatgccga ccgcgccgat cttttgcccg
     3301 gctttgacca actcgttaac cttgacaagc gttttgccga gatgagtata gacggtatag
     3361 agggtatctc catctaattc catatcatgc cgaaccatta ctgcgatacc gtaggggtca
     3421 tttctcgctc cctcgccata aagcaaaccg taccctgaga aaactactgt gccatcggca
     3481 gcggccagca ccggggtttt ttccggcgcc gggatatcca atccgccatg cacaatgctc
     3541 tcctgcttta tgaaataccc atatcggtaa tcagccgtcc gccaggtgag gatttcatca
     3601 cggatggggc gagaaagcca aaaatggtcg tggggggtta aggatagcgg agcctgaaaa
     3661 aagggtttat tagaagggta gtaagggtct tcaggggtgt cacccagccg gagcgcatca
     3721 ccctgggaag atgaattaac gtttgtgctg aaaggcaggt tgtttaccgg cgcggcagcg
     3781 ctgataaaaa agaccgatat gatgaagcct atgaccgccc atctttgagg aatgcctgaa
     3841 ggttttttca tgggagcgac cagtaacttt cgatggtagt atcggtaaac cgttgcctca
     3901 tggcctcatg ccagctaagc ccatcacgca atacgaagcg gttgatacgg gtttcaggga
     3961 aataaacctg ccagtttgat ccggccggca gcctttccca gccgtacccc agggcgaagg
     4021 aagtgaaatc gatccaatac ccctggggga gggttgtggc caggccgcca ttttcatagg
     4081 cagaagagga accctgaatg cgggcattaa aatcccagac cgggttctgg ataggtacac
     4141 cgcaggttcc atcttgctgc atgcacttaa gccacacacg ccagtaggtg cgcccaagga
     4201 tatcctcacg gctgacagcc atccaaccag aatcgagcgg gtccaggttt accgtgatgg
     4261 ctcttccggt atatccccag tcttcgcgca accctgatga agagtaagca ggaatagaaa
     4321 tgctggcgct ctccagtatt ttgagaaaat cccaacccaa cttagagcgc aaggcagcgc
     4381 gcatactgtt aaagctttcg ttgactgtat cgatgaggta tggttcaggc gcggtgacat
     4441 ctggcaggta taccatttca taacgaccag aactgacatc ttgtgtgctt atctcaggct
     4501 gccataatct cgtttcgctg gcaggggagt gttgagagag gtatttctta agaggaagcg
     4561 ctgattttgc tggcagccaa ctgatggaca gtacctcatt tttctgatgg atgggaggca
     4621 gcagcaggag gttgggttca gtgctgtagg cgacaaggtc gttgctgttg gcggaatgaa
     4681 ccacagccag cacgccttgc tcttcatcca tccacactgg atgcgaacct gaccccatgg
     4741 taacaggcct cgtttgttcc gactcgaggt tccaggcttc gatttcttcg aattcatctg
     4801 tccttaccca ggccagcgaa tgaccatcgc ctgaccatac agggtgagtg taatccgcat
     4861 gttctgagcg cgccacgaga ttaaagcgtt gctgaggatt atccaggttt gccagccata
     4921 actccaacct gccattgcgg tcagtgacga aggcgatctc gcgccctgag ggcgaccagg
     4981 ctgggtcaaa attatttccg ccattgtcgg tcagcctgtg cggcgcgaca gattcatcca
     5041 ttacggaacg gatgaccagg ttgagtttcc catccatcac cacctcgtaa gccacccagc
     5101 gtccatctgg cgaccaggtt ggggagcagg cgtaggtggt tgcgttggtg aggttggttt
     5161 tctctccggt aggcaggtct aggatgaaaa tatcccagtg gccaaattca tccgcgcaaa
     5221 aagcaagttt tgtgccgtcc gggctgatgg ccgggtctgc gtggtcccat tcatcatccg
     5281 ttatctgggt cagggcctgg ttcagggggt gataggctgc gagttgagcg aagccgccga
     5341 tctttacagc gaggatgatc aagccgctgg ttgccgggtt ttcagaggtt gcaaccgacc
     5401 cgggcgaggt ggtggggatg agagttgggg ttgatagcag cggcgaggcg gtctcagagg
     5461 gttccggcag gctgacgact gtgccctcga cgtgggcagc gatttcaggc gcggcaggtt
     5521 caggcttgca ggctgatagc gataccacga ggaatggtat cagcaagatt aagcgtgtgc
     5581 acagacgggc gctggtgttc atcttactat caagttttct tcctctgttc ctcaggagat
     5641 cgcgctcagg gttcctgttc ccaaagaatg acagggttaa aagattggaa tctccctgat
     5701 gatgaccaga tgcaatatct gtaccgttct tcaatagtgg catcgggtga gtacatggcc
     5761 agtgttccaa attgtgcacc tgctggcccg accctcgctt caagatgaag atgctctgct
     5821 gccgagttgc cagtattgcc agcggcgccg atcacttgcc cgcaattgac cacatctccg
     5881 gctcgcacgg atggtttttc caacaagtgc gcgtaaagca cgtatactga tttgcggcta
     5941 ccatcccatt caatcggcgt catgtcagcg aacaatgggt ctgaatcaca tgggcgcaca
     6001 agatccaaat tctcctgtgg tatcgcggtg gggataggca atgcgcttac ccattcagcg
     6061 gataatacgt tgagtggtgt ttcgatgaga acagcatttc cgtacgggaa acgatcaacg
     6121 attaccaggt tgacgatacc gggaaagatg gattgcaccg ggtgaccaat catcgatggc
     6181 agttctttga aagtgaaaaa agccaggtcg atcgcggggt gtccatcatc tttatatggg
     6241 gcaggagggg tgaagccttg agacataatt ttatgcagat cactgagttc aatgtccttc
     6301 agcggcgagc aggcttttat cgcgggctgc agcgtggccg tgaaagcagg ggttggattt
     6361 tgtgtggctg tgtgggaagg agagggagtt gttgtggcat gaaaggttgg cgtcgtcatg
     6421 accggtgttg gggttggtgg ctgaggaaag gaactagaac aacccgtggc taccagcacg
     6481 aaacttaagg tcaggcaaag gatgacagcg cgctgctttt tcatattcat cttctactgg
     6541 gagcccactt ctactggtac cttttttgag tccgaaaact cgttgtcatt ggggaagaca
     6601 accgcctgta aaaataaacg atcgttggga ctgtgttgac ccaggtgcaa ggtaaacgaa
     6661 actagcgggt tgaatgtgcc gatgatggtc gagcgcgcga tctccatttg cttttcgtca
     6721 agcaagctga gttcaagagt agggcgacat tccacgttat tcaaaaccag tgtgacacgc
     6781 acgcgttgat tgtctgggag taattttgcc tgaagacgat cgatttgtgg cggcgctgtc
     6841 acctttgggg agttgtgggg agtattcatc agcctcatcc atttagatgg tgattttaga
     6901 tggtgatttt ccgcgattcc atgtaaaaac gtttttcgtg tgcttcaccc atggaaaagg
     6961 ttttcctcgg cagcgcgcca tcaagcatca ctgtgcggaa cagttcatct tttgacattc
     7021 ccggtaagta aatgcctgtg ttggcgggtt tcatccccaa ctcgcaaagg gcttcatctc
     7081 catgcacata gtctatactg gctacggatc catccgcaac gagctgatcc aagatggtct
     7141 gcagcgtacc caccaccagg ttagcggtag ggctggtgat actaccggcc acaagtcccc
     7201 gcgttgagac caaaccaaac cgctggcatt caaccgaggg ttgttttacc tttgtggtca
     7261 attcggtaaa agaacttact ggttccaatg ttagcgcccc atcatatatg cccttaagga
     7321 cttgttcgag attgacttta ctgttgaaaa gcacgcggtg aatcggctcg aatgccagcc
     7381 cggcgtcatg gatattttcc acctccacaa gggcgaagcg cgcagggtgg tccatgctta
     7441 cgctgctttt cagctcttcc caaatcgctt ttgcggttgc cagggagtgg ttgccatcac
     7501 ctacagcgaa caacaatacg cctttatcgg gagaaacccg gtatttcgcg taaaaggtct
     7561 gtggattagc cagcgcggcg aggttttgga tgattgattt ttccagcgcg gaatcattta
     7621 ccagcctgct gcgtaaatgc ccgccgcgct gcatcagatc aaaatcataa gccagcggta
     7681 aacgctggct ggcctggacg acaggaccga tgaccctgtc ttctgggtca tcaatgagaa
     7741 cgacgatgtg cggcagctcc aggggggcgt tcttgcggat acggatacga ggaggaagac
     7801 ggtcaaggat cgtgccttca gtggcacgga tcaaactttg agaacctttc gagaaatcgt
     7861 aatgctccag gtccagggcc agcattaacc cgcgccgcgt ttttaccccg atggtacgct
     7921 caacgtagat gatattttgt tgaggtctta acaagccatc gctgaggtat tgttgcattt
     7981 tcgcatgcgt actttgaacg cgttctgttt ctttgtcggt gccgagatag acttcaggca
     8041 aaataaggtg aaaagttgaa gggcaatcac ctacgttttt cgcgacctgt tcccagtatt
     8101 caggttgtga agtatattga tcacaggcga tcacggccca cttcgttaga tcaatttttt
     8161 ccaccggcag gtaaatttct ggaacttgga caccaatttc aggataattc ctcatctttt
     8221 cagggctcct ctttttaatt ttaccagagc ggcatatcat aaaaaaacct cccgttgccg
     8281 atgggaggtt gttaagtatg tgtaaagcgg gctacatgct gtgcatgaaa gccacgccca
     8341 aagcgccagg cccagcatgg gttcccagca ctggcgagac ggtgctcaac actgcgtctg
     8401 tgaccaggct ggaggggaaa cgtttgattg ctctttctaa cagcgcctgg ccttcttttt
     8461 cagcgttggc atgaaccacg cataaacgca ccggaccctg tgctgttccg acccgttgtt
     8521 caaccagatc aagcaggcgc tcagtggctt taccaatggt cctgacgcgt tcaaccgctt
     8581 caatccgccc atcgcgcagt tcgaggatgg gtttcatgtt caacatagtg cccaaaaaag
     8641 cttgcgcgcc gccaatgcgc ccgccgcgat ggaggaattc cagcgtgttc aatacaaagt
     8701 acacacctgt attggctttt gctttttccg caactgcctt gcattcttcg agggtggcgc
     8761 caatccctgc catgcgggca gcgctgagca cctggaaccc catggccatg ctggtgctga
     8821 gggagtcgac cagttctatt ctagcgcccg aaaaagcatt cttcgcctga atggctgaat
     8881 cgagtgtgcc agagagtttg ctggagatat ggatactgag gatgtcatat ccttcttcta
     8941 tcagcctgcc gtacatcgct ttaaattctt caggggtaac ctgcgaggtc gacggcatga
     9001 catccgcaac agccaaacgt tcataaaatt ccttcgcctg gatatcaacg ccatcccgaa
     9061 ggatttcgtt tccccagatc acctgtaagg gaacggatac aatcgggttg ccatcccata
     9121 attcgggggg aatattggcg gtgctgtctg taacgaccat cactttattc atgaaaatac
     9181 ctccaattac tcgtccttgg ttctttgccg tatataaccc cgcaacgaaa aaaccgatgc
     9241 acgcaaatgg ggtggttttt cgaagaagtg catcttgacc aataggtgct tcaagactaa
     9301 aatccctgct gaatttccat catgggacgg gtggacgacc agggcgtatt cgattgtttg
     9361 aaaatctgac cggcaggctc aaccaaatat tcacccagct gcggcgaaaa ggcaaacttt
     9421 ctgctgctga tgtggatgca atgatgcgcg aggtgcgtct ggcgctcctt gaggcggacg
     9481 ttaactacac ggtagtaaag gacttcacca cccgtgtgcg agaacgggcg attggcgccg
     9541 aggtaagtaa ggcactcaac cccgcgcaac aggtcataaa aatcgtgcat gaggaattga
     9601 tccatacgct gggtgaacct gcccggttga acctcagcgg ggtgaaacct tacgccatca
     9661 tgctggttgg tttgcaggga tcaggcaaaa ccacagccgc tgccaaattg gcgaaaaggc
     9721 tgcgctcgga tggagagcgg gtattgttgg tggcaggcga tccttaccgc ccggcggcgg
     9781 tgaaacaact tcaaaccctg ggcgagaaaa ttggcgtcga agttgtcgcg gattcaactc
     9841 tcaagccacc tgcgctggct gcgcacgcct ttgataaggc gcaaaaaggc ggatattctg
     9901 tcgtcattat tgatactgcc ggacgctcgc agctagacca gacattaatg gacgaattac
     9961 aatccattgc caaaaaagtc agactggtag aaaccctgct ggttgttgat gcgatgatcg
    10021 gacaggaagc gctgcatgtg gctgaaggct ttcgcgatac ggtaaatatt accgggctgg
    10081 tcttcaccaa gattgatgga gacgcgcgtg ggggcgcagc gatttccgtt cgcaaggtca
    10141 caggggtgcc gatcaaattc ctgggaacag gcgaaggcat cgacgcggtg gaagtgtttg
    10201 atcccgccag attggcgtca cgaattctgg gaatgggtga tatgattggg ttaattgaga
    10261 aagcggaagc cgctttcgac gagaaaaccc aaaaagagca ggcagaacgg gttctcaaag
    10321 gtcagttcaa tcttgaagat tttgctgacc aattgaagca actgcgcaag atgggtccga
    10381 tctctcaggt gatggatatg ctgcccggcg ggttagggca ggcttcgcgg aatatcccac
    10441 cagaagaaac cgagagaaac ttgaaacgca ccgaggccat tatcgcttcg atgaccaaac
    10501 aggaaaaacg ggaccccgac ctgttgaacg cctctcgccg ccggcgaatt gccagtggat
    10561 cagggacaaa cgtgatggaa gtgaaccgtc tgatcaaaca gtttagggat gtgcagcggt
    10621 tgatgaagac gatgcaaaaa acaggcggga agggactttc ccgattgttc aagtgatcaa
    10681 aaatgaaaca gcggtaagcg cttccttcag cgcccctcgc caaagcggaa tttttggatt
    10741 cacatcatat ttgatcattt tttgagaaac aggagataag caaatggtaa gaattcgttt
    10801 acgcaggaca ggtcaaaggc accagcccag ctaccgcatc atcgtggcag ataaagaaag
    10861 cccgcgggac ggtcgtttcc ttgaaatcat cgggcactac attcctacga ctgacccctt
    10921 cactttcgaa gttcaggaag atcgggtgta cgagtggatg aaaaatggcg ccctccccag
    10981 tgattcggtc ttaaagctct ttaaaatcgt tggtgtgctg gaacgttatg aaagatttaa
    11041 aaaaggagaa tctctcgaga cccttctggc agaagccaaa aaagcgacag aagagcggaa
    11101 cgccaagatt aaagcctgaa caggatcatg atgcagccaa ccaacagacc cacacaacgc
    11161 tattcgcgca atgtcaacca ggaaagtcac atcaaagagc tggtggaata catcgcgctt
    11221 tctcttgtag atgatccttc ccaggtggtc gtcactgaat accgctcagg aggacgggaa
    11281 aacctggagc tgcacgttgc cagggaagac atgggccggg tcatcggcaa aggcggcaaa
    11341 attgccaacg ccatacgggt gttattacgc gttgccgcag cccgtgaagg caaacaggtc
    11401 aacctggacg tcgctgaacc gagatgacca atcgcaagtc aacgggtcct caattaaaga
    11461 cacaaccagg ctcgcctcaa cccggcgagc ctgtctactt gatgattggc aagcttcgcc
    11521 gcccacacgg ggtaaaggga gaactatggc ttgaactgac cacagaacac cctgaaagaa
    11581 tccgcactgg tgtaacgatt ttgattggcg atgaaaagac ggaagtgaag gttgagaata
    11641 tccgtgcggc tgataagcta tggttgatca gttttgccgg ctaccaggat tgccaggagg
    11701 ttgagggatt tcgtaaccag tgggtgttca tcgaagcgaa acaggctgct ccactgccgg
    11761 cgggacggta ctatcatcac gagattatcg gtatgaaggt ctttactgaa acgcatgacc
    11821 ttataggagt agtcgacgaa attctggtta caggcgcaaa tgatgtttat gtggtaaaaa
    11881 atgataaagg caccgagacc ttggtgccgg caataaaatc ggtaatcctg gaaattgaca
    11941 aagagatggg ccgcatgatc gtacgccagc aggagtggtt ataggaggct gagttggagc
    12001 cggacaaata ctgggtaggt ttcaacctgg tcaagggtat tggagctgtt agattcaaac
    12061 aggtcctgga tttctttggt tcggctgaaa ccgcctggtt cgccccggtt tctggattgc
    12121 aggctgccgg tttaccccaa cgagtgatag agaatttttt gcttgtcaaa tctcaggtgg
    12181 acctggagcg ggtgatggaa aggattcacc attccggcac cagggtgatc acctggaatg
    12241 atcaagatta tccccgccgc ttaaaagaaa tagatcagtc tcctcctgtg ttgttcatca
    12301 gaggctccat caatgtggaa gatgattggg cagtggcagt ggtaggcact cgccgggtta
    12361 caccttatgg aaggcaggtc gcgaatgaaa ttgccacctt cctgggtcag aatggcgtga
    12421 cagtcgtcag cgggttggca cgcggagtgg atgcgatcgc ccatcaatct gcgcttgcgg
    12481 cgggtgggcg cacaatcgcg gtgttgggta gtggtgtgga tgttatctat ccgcccgagc
    12541 atcgcaagct ggcggtcgag atcatcgctc aaggcgcgct ggtcagtgat tatcccctgg
    12601 gcacctcacc agatggggtg aatttcccgc cgcgcaaccg catcatttcg ggtttgtccc
    12661 tggcgactgt agtggtggaa gcaggagaga aaagcggcgc gctcatcact gctgaatttg
    12721 ccgttgaaca gggtcgagat gtttttgctg ttcccggaag tattctggcg ccgcaaagcg
    12781 caggaaccaa tcaattgatt gagcaagggg cccggccgtt gttgaaaatg gctgaaatac
    12841 tcgaagtgct taaactggaa cagataccag aaaagcagca ggcgcgaaaa cttaacccgc
    12901 tgaaccaggc ggagcagaac ctgttgcagc acctctcgac agaaccgctg catattgacc
    12961 aactctgcga gttgacgggc ttgccgattc atgatgtatc agcaactctc accatgatgg
    13021 agttaaaggg attcatttgc caggtaggcg gcatgaatta tgtggcgatg cgcgaaaatc
    13081 gaggtttgta caaggtgaat aaatgaagat agaacattat tacgcggtga tcatggcagg
    13141 cggcggaggt accaggcttt ggcccttatc ccgccatgac tctccgaaac agacgctacc
    13201 aatctttgag ggcaagtcgc ttttccaatt ggcggttgaa cgcctgcagg gatatttacc
    13261 ggtaaatcag atctatgtag tcacttctgc tgacctgatg gaacagctgc acgcgcaggt
    13321 tcccgaactg ctgaccagca attttctcat tgaaccacaa ccgcgtggaa cagcagccgt
    13381 ggtcgccctg gctgccgcga caattttaga aagagaccct aacggggtac tggcggtatt
    13441 aactgcggat cacctgatgc ctgataaagc agaattccag gcctgtctgc aagccgcggc
    13501 agaactggca gaacagggat atattgttac catgggcatc aaacctgctt cgcctgcaac
    13561 cggttatggc tacattgaac gcggggctga gcttggggtg tattcaggca gggtgggcta
    13621 tgaagtattg aagtttattg aaaagccgca tcaatccaaa gccaacgaat tgatcaagca
    13681 ggaacgctac tcctggaatt ctgggatgtt catctgccag gctcaggtgg tactgggaga
    13741 atttgagcgt tacatgccgg aactgtacac ggtcatacaa tcgctgcaac catttctcgc
    13801 gagcgaccat ctggctgcgc cttttgcggt aaagtggttg aagattcaac ctgaaacaat
    13861 cgattatggc atcatggaaa agacctcgct ggcagcggtt cttcctttat cgaccctgga
    13921 atggatcgac gtgggttcct gggattcgtt ctttgaggtg tttaaacccg atgagaatgg
    13981 aaatatcatc ctgggagtta aatatgaagg aattgacacg gctgattcac tgatcctatc
    14041 gacagatacg gaaagaccgg tggtaacaat ggggatgaag aatatgatcg tcatccaagc
    14101 ccctgatgta ctgatgatat gcccgcgagg ggaaagtcag cgggtgaagg aactggtaaa
    14161 ctacctgaaa gccagcgatt atacccttat cttataggag agccgactgt ggaagcctat
    14221 tgcgtcaagt gtaaacaaaa aagagagatt gaatctccca acgcagagtt caatgcgctc
    14281 gggacgcctg tgacccgcgg aaagtgccct gtgtgcaaca ccagcctgta tcggatagga
    14341 gaaacgcctg ctcacgccgg ccttgaacga ccaaagagac tgcacgccaa accgcgcagc
    14401 ggcaaactgg tgattgtaga atctcccgca aaagcgaaaa cggtggggcg gtttttgggc
    14461 aaggactaca cggtgcgggc ttccgtgggg catgtgcgcg atctgctgcg ttcagaactt
    14521 tctgtagacg ttgaaaacaa ctttttgcca aaataccggg ttccgaacga aaaacgaccg
    14581 gtcgtgaaag aactgaaagc gctggccaaa gaggcggcag aaatctacct ggcaacagac
    14641 cctgaccggg agggagaagc cattgcctgg cacctgttag aagctgccca gatcgaacca
    14701 aaactctcca ggagagtggt ttttcatgaa ataactgacc acgcgataaa cgaggcgttt
    14761 tcacagccgc gcgaaatcaa tatggacctg gtcgacgccc agcaggcgcg ccgcatcctt
    14821 gatcgattgg ttggctattc catcagcccg gtgttgtggg agaaggtgcg cagccgctta
    14881 tctgctggca gggtccaatc agtggcgctg cggctcattg tggaacgcga gcgggagata
    14941 gaggtgtttg tcccggtaga atactggtcg atccatgccg aactcacccc cgagggcaaa
    15001 aaaaccagtt acatgaccaa actggcaaag atcgatgagg ttgaccccga cctgggcaac
    15061 aaggaggttg tggatggtta cctggcggat atcgaggcat caccctttgc ggtaacccgc
    15121 attaaaaaga gcgaacgccg ccgaaaacca tcggcgcctt ttatcaccag caccctgcag
    15181 caggaagctt ccaggaggtt aggctacacc gccaggcgca ccatgatgat tgcccaacaa
    15241 ctttatgagg ggctcgaggt tggcgagggc ggcgcaactg gcctcattac ctatatgcgc
    15301 accgattcaa ccaatgtggc agaagtggct caagaagaag cccgctcgtt tatccaacag
    15361 acctatgggg ctggctttct gccagaaaag ccaccgcaat ataaaaccag agctgtttca
    15421 gcccaggaag cgcacgaagc catcagacca acttcggtac tgcgccaacc tgaaaagata
    15481 aaggagttcc tttcttccga acagtccaag ctataccagc tgatctggca gcgcttcgta
    15541 gcatcgcaaa tggaatccgc cgtttatgac acgatctctg tagaggtgac cgccactggt
    15601 aaagatcatc aatatctttt cagggcatca ggctctacca ttaaattccc gggttttttg
    15661 atcgtgtatg aagaaacagt tgatgaggac ctgaaaacag aagaaacaga aaatgtgcgc
    15721 attccagccg ggttggtgga aggtcaggcg caacacctta aaagacttat acccgaacaa
    15781 cactttaccc aaccaccccc tcgctatact gaagcctcgc tggtacaggt tctggaggaa
    15841 tatggcattg gacgcccttc aacttacgcc ccgatccttt ctaccattca gcagcggggt
    15901 tacgtgcttc gcgaggcaaa aaggctttcg cctactgaga cgggaatttt agtcaatgac
    15961 ctcgtggtcg agcatttccc ggacattgtc gatctgaact ttaccgccgg tatggaagcc
    16021 cacctggacc aggtggccgc gggtaaagaa gactggcata aagtgttggc ggatttttac
    16081 actacatttg aacctcaggt caagagggcg caggcggaga tgcctgttac caagacagag
    16141 cccgaaaaaa ttggccgcgc ctgcccgaat tgcgggcacg acctggtcat ccgctggggg
    16201 cgatatggca agttcatcag ttgctccaac ttcccggctt gccgccatac agaagccctg
    16261 ctggagaaaa ttggcgtgac ctgcccgaag gacgacggcg acgtggtgct gcgcaagacc
    16321 cgaaagggga gaatcttttt tgcctgcagc aactacccta attgcgactt caccagttgg
    16381 aaaagaccgt tacctgtacc ctgtccaaaa tgtggcgggt ggatggtggt agcgaacaag
    16441 cgtgaagccc aatgcatggt ttgcgaggag tctgttctga tcgaagagct cggggtgcca
    16501 attcccgatt aggcataata attgcaacgt agttaacgag ataactggat caactactat
    16561 ctccaggagt taactatgga taagttgcgt gatgtttttt tcaagttgat cgataaacca
    16621 ctatatacag ccatcgccgg cttcgtgatc ggcctgatca tcggcttgcc catattgggt
    16681 tggtggctgt ggccagtcaa gtggaaagat gcggatgcca gctttttacg ccaggacctg
    16741 aagaaccagt acctctgtat ggttgtggat tcatacagtg tgaaccatga tgccgcactg
    16801 gctggcgcgc gcataaattc gcttggcgtg gatattaaga aaaacccatt cctgttcgat
    16861 aacctggctg caggcgggtg caattacgca ccaaccgata aggtaatcca gagcctgaaa
    16921 accgctttgg tgacgggtga gcaggttgtg ccggagacag aagcaaacaa tcaaccggat
    16981 ggcgatcagg ctgagatcaa ggcaacagaa ccattggcga ctgcagaaat cgcgggttcg
    17041 acggccattc ccttacccgg gaataatgcg ccaaaagaga aaaaaggcgg aagtgtgttg
    17101 cttttatccc ttctttgcgt ggttttcctg cttatcggtg gggcattgat ctacatcttt
    17161 ttcatccgca atcgccgcac atcagaagaa attcctcacg catgggatga agaagaaaga
    17221 ttcgctcaag atgagcccta cgacgatgcg cctgaatacc ccgctgctgc aacaccgccg
    17281 ccaccaacgg ccattcatca cgaatcggtt gatacgccac cgatggatta cttcagcgaa
    17341 cccgaggaaa tggacgatgt cgacgagatg gaaattcctg acatccttga gggggctcag
    17401 gatgttgcca aaacccctcc attgaagagc cctggagtgc gcgctacgct tcctcatcat
    17461 tctgacccgg tggctcactt tatgaccacc tatgtgatcg gcgatgacct gtacgatgac
    17521 agtttcagta ttgacgcacc gaacggctca ttcctgggtg aatgtggtgt aggcattctg
    17581 gatacgatcg gcgtgggaga cccgaagaag gtaaccgctt ttgaagtatg gctttttgat
    17641 aagaatgaca tccagaccgt taccaaggtg ttgctcagcg ctcatgccat gaacgatgcg
    17701 ggagtgaagc agcgcctggc ttcgaaaggt gagttggtcc agattgaatc cggtcagcaa
    17761 ttactattgg aaacggccac gctccagctt gaggcccggg tggttgaaat ggtttatggg
    17821 cagggtgcgc tgcctgccag tagtttcttc cagcgcatga cgctggagct ttcggtttgg
    17881 ccgaagtaat accagacgat tgaagaataa tccgggagaa ccccggatta ttttatttta
    17941 cttttagaat acggaaatgg agttctccgg caggcgtgtt ggcggttatc ctgtcgcctt
    18001 cttggtgccc taagagcgcc tgaccgattg gcgaatcata agagatcttt ccgcgccgtg
    18061 gatccgcttc ttgaggaccg acgatatgga aggtttcttc ttcttcctgg tcctcctgga
    18121 tggtcacggt cgagccgatt tgaattttgc ctgtgtggct ggggccatct tcaatgagca
    18181 cgacgtcgcg caatatctgc gtcagttcct ggatacggcc ctcaacaaac gcctgttctt
    18241 ctttagccgc cgcataatca gcattttcgg agaggtcacc catctgaata gcagctcgca
    18301 gccttttcgc cagttcctgc ctgattgggc cttcaaggtg tttcagttca gcgcgcaact
    18361 tctcagcgcc ttctcgggta agatatgaag gttgattcat gtagactccc ttgggtgcga
    18421 ttataactta cggcagcagt gagggatcga ataacttttg atattcgtca acagcaaaac
    18481 ggtctgtcat tcctgccagg taatcgcaaa tggtcttttc ttttcctttg atctcgagct
    18541 gatcctggaa atgtattggg agcatgttgg gctccgaaac ataggcgtgg aacaattccg
    18601 ataaaattcg ctccgccttc ttttgcatgc gcacgacccg gtaatgccga tacatgttct
    18661 tatagaggaa atctttaagt tcgcggtttc gtgcgcgcat cacgtcatca aaccctacca
    18721 cgttttgagc gtgcgactgc aagtcagcga gtgttttaac accacttcgc tgaaggcgct
    18781 cattggattc actgatcatg ctatccactt ccaggccgat cagctcgcgg atgatactgt
    18841 ggcgggtcag gtcatccaac tggcgggaat gaatattgag cctttccatt accatgttcc
    18901 acaggtctat ctcggataac atatctgtgg tgatcatccc cgaacgcaac ccatcatcca
    18961 ggtcgtgagc ggtgtaagcc agctcatcgg cgacattggt gatttgagct tccaggtgac
    19021 cgcgcaaatg cgggtcataa tccagcgcgt cggtcttatc atattcggtt tcatgcttga
    19081 cgatcccttc gagcacctcc caggaaaggt tgagtcccgg aaactgcgcg tagcgatgct
    19141 ctaattccgt cacgatgcgg taagactggt gattatggtt gaacccgcca tacccttcca
    19201 tcaattggtt cagggtggtt tccccggagt gtccaaaagg cgggtgaccg aggtcgtgcg
    19261 ccatgcagat ggtttccacc aggtcttcgt ttgcacccat cgcccgggcg attgtgcgac
    19321 cgatttgcgc cacttccagc gtatgggtca ggcgggtgcg gtaataatcg ccctcgtaat
    19381 taatgaacac ctgggttttg tattccaacc tgcggaaaga ggttgtgtgt agaacccggt
    19441 cacggtctct ttggaaacag gtacggtaca gcggctcgtt ttcagggtat tttctgcctt
    19501 tggagcggct ggcgtgaacg gcgtaagtcg ccagggtttc ttcttcaatt ttttccagat
    19561 aagcgcggtc gaatttcatg tcactatcct tttttatcca gggttataca ggtgcccact
    19621 tttctcccgg tcaacgcctg gaacacgttg ccctcatcaa gaccggaaaa gatcaatacc
    19681 tgctgacagt gcaggttcgt cacaaggttc atcatgttgt gaaccttact acgcatgccg
    19741 cctgtcacgt ccgggctctc tgatgattca aggtgaggat ccaccatggc aaggtttcgc
    19801 ggcgtgatgg catccagcag ttctttcctt ttaggaaaat ccatccaaac acccttttcg
    19861 ataccggcga gcaaaacgcg agcaggcttg aagatcgctg ccagaaattc aaattgctct
    19921 tcagtggata gaattgtgcc gccgatggtt tgatcaaaga ccacatcacc gtagaccact
    19981 ggcagaatgc cattatcgag acaagcagcg atttgcgtcg tgtcccagtg ggtgattttg
    20041 tgagaagagg tgatgacctg ggcgcttggt ggaaaagcca tcgcgtgaac gccactttca
    20101 tgtaaaacct ccatcacgat ggtattgaga gcccgagctt catgccaaac ctctaccaga
    20161 ccgcgccact gtgcaaaagt ggcaacgcca tcacgtgttt ggtatttttt cgcggggata
    20221 tgcccaaagg aacctgaacc atgccctaaa agtaaccgaa ctccaggctg atcggtcaat
    20281 gccctggcaa tttcatccgc caggcgcttc aacacatctg acctggcttg gtgcgggatg
    20341 tttttatttg tgatgagaga gccaccgagc ttgaggaaaa agaggggagt tgtcatcagg
    20401 cacctcgccc tggtgcgagg gtggtggtga tggtgcgcac ggctccctgc tgctctaacg
    20461 ccctggaaat actatctgct tgcgtatggt gcacaagcgc gatgatattc ccgccaccgc
    20521 ccccaccgga caactttgcc ccaagcgccc ctgcttgaag cgcgcagttg accaaatcat
    20581 ccagcattgg agtggagact cccagcgctt gtagaagttc atggtttctg ctgagcagct
    20641 cgccatgctg gttcagatca tttttttcga ggttctcttt cacctcccgc gataggtcag
    20701 cgatgttggc aaagatggcg tcgtaacgtt ttttttcggc aagccagcgt tcgcgaacgc
    20761 gggccacggt ctctgccgtc aggctggcag cgccagaatc agcaatcacc agcgtgaagg
    20821 gttggcgaat cttgaggtgt tcaatcggtt tccctttaac aaagtaaacc ggtacctggt
    20881 aggtgacgac ggtgttgtcg atgccggaag gcgtaccgtg atgcagtttc tcaatttcaa
    20941 aggcgacttg attgaccagt tcatcggata aggggctgcc aaggaaggca gacaacgccc
    21001 gcgcgagcga tacggttacc gaagcgctgg agcccagtcc agtggcgatt gggatggtgg
    21061 tgttgatgcg tatctcacaa gcctgaagcc tggtaacccc cattttttgc atcaccaggg
    21121 cggtggttgc ttttaatggg tggttgtacg cgagagattc aaggtcacta tcgagaccaa
    21181 tacagggggc tatcattctc actttgcctt caggcgcggt tggctgggca aggatggtgg
    21241 ttttcgtgtt aagctgaaat acaggcaggg cgatggcggg agcgccataa acgacagcgt
    21301 gctcaccaca aaggataacc ttgcccgggg atgatgatgt gatggctggc acagctcacc
    21361 taaaaaaggt aaaaaatcac catcactgcc accccccaca agagcacggt caactggatt
    21421 ggacggtctt tgagcaagat ttcttcaggc tcaccacccg cgttgtgttt gttgatgagg
    21481 aagagataac ggaaaatacc ataaagcaca aacgggatcg tcagcatcat cgaatggttt
    21541 tcaggaaggt ttggggcgga gaaagtgtaa agagaataag agatgagagt agtagcggaa
    21601 acaacggtaa tcagctggtc aatgaactgg atagaatagc ctgaaagcac cttacggtgc
    21661 gattgagggc tgtcattcac cagtaccgcc agttctgcgc ggcgcttgcc gaaaccaagg
    21721 tatagcgcca gcagggtagt gacgacgtaa agccagggag agaagcgctg tacttcaatc
    21781 acggaaaccc cggcagccac gcgcagcaca aaacaactgg cgataaccaa cacatcgatt
    21841 atcgggatat gctttaacca gattgaatag attagattaa gcgtaaaata caccagcgat
    21901 atgaaaaaga aaccatgaga gagcaagaac gctcctgtca gcgagaccag caagaggaag
    21961 atgccggtgg ctaaggccag acccactggc agattgccgg aggcgatggg gcggttcttc
    22021 ttgatcgggt gcgcacggtc agattcacgg tccagaatat cgttaatcag gtacacgctg
    22081 ctggaaagaa ggcaaaagag cacaaatgca gccagtgttt gcaaaaacgc gtggagattg
    22141 agcaatttgc ggtcgaagac cagggcagca aaaatgaacg cgttcttggt ccattggcgg
    22201 ggtcgcattg ccttaaggat gtacttgagc atgaggataa tatatcacaa gtccattgtt
    22261 cgcgattaga attgggttat gcaaaaaacg cgtctggata ttctgatgga gtcacgtggt
    22321 ctttcaccca gccgttcaca ggcgcaacgg ctgatcatgg ctggcagcgt acgcgtaaat
    22381 catcaggtgg tgctgaagcc cggtactaaa gtggacccgg gggatacgat tgaaatcagc
    22441 gaatcaccac gctttgtttc ccgcggcggt gaaaagttac tttccgcgtt gcaggctttt
    22501 catctgacct ctctggcgga cagggtttgt gttgatgtgg gtgccagcac tggcgggttt
    22561 accgattgcc tgttgcagca cggagcaaaa aaagtattcg cggtagatgt tggctatggc
    22621 gtgctggatt ggaaaataag gaatgacacg cgggtggtgg tgatggagaa acaaaacgcg
    22681 cgctatatca ccacctttca tgaaccgatc gacctggtgg taattgacgc ctcgttcatc
    22741 tcattaagaa cactgctgcc agttgtgcgc caatggttga acgccccaaa aggggaggtc
    22801 gttgcgctga ttaaaccgca atttgaagcc ggccggagcg aaactgcccg gggcagcggc
    22861 gttattcgtg accctgaaat tcatcgcgca gtattacgcc aggtgctgga atttgctcaa
    22921 aaggaaggtt acaccgctga aggtttgatc agatctcctt taaccgggcc gaaagggaat
    22981 attgagttcc tggcgcacct gatctaccca ggtgagacgc ctgcagatgt tgaaaggctg
    23041 attgaggggt taccatggaa cgagggaacc accgctgatc aaccctgcca accggaacct
    23101 gaagggaaat tgaaataagg tatactttat tccggtatgt ccacagaaag aattcgcaac
    23161 ttctgtatca tcgcccacgt ggatcatggc aaatccacgc tggcagaccg catgctgcaa
    23221 ctcaccggca ccatttcgga cagagagatg acggaacagg tgctggattc gatggacctg
    23281 gaacgcgaaa aaggcgtgac catcaaagca tcagcggtgc gcatgaacta cgttcatgct
    23341 ggtgagacgt atgaactaaa cctgatagat actccagggc atgttgattt caattacgag
    23401 gtgagccggg ctttggaggc ttgtgaaggg gcgttactgg tggtggacgc cacgcagggt
    23461 attgaagcgc aaaccctggc caacctgtac ctggcgattg aatctaacct gaccattctg
    23521 ccggtgatta ataaaattga cctggtctct gccagacctg atgaagtcgc ggaagatatt
    23581 gagtccctgt tggggttgcc ggctgaagag atcatccgag tttctgccaa ggacggtttg
    23641 aatgtggacc aggtgctgga tgccattgtc acacaaattc cgccgccgaa aggggatgat
    23701 accgccgcac tgcaggcgtt gatttttgac agccattacg attcttataa aggagtcgtg
    23761 gcatatgtgc gcgtgatgca ggggatcatt aacgcggata ccactctgcg gttgatggcg
    23821 acaaaagcag acgtaaaacc ggttgagata ggtgtcttcg ctcctgggat gatacctgtc
    23881 ccgttgttat cggctggtga cgtgggctat gttgccacag gcttgaagac cgtgaaggaa
    23941 tgccgcgtgg gagatacgtt tacgctctcc tcagagccag cggcggcccc cctccccggt
    24001 tataagcacc ccaaaccaat ggtctttgcc ggcatttatc cggttgaagg cgaggattat
    24061 gaagcgttaa aagaagccct tgagaggctt cagctgaacg atgcttcgct ggtctttgag
    24121 ccggaaactt ctcaggcctt gaattttggc ttccgttgcg gctttttggg cctgttccac
    24181 atggagatta ttcaggaacg tatcgaacgt gaatataacc tcaatattgt ggtgaccgct
    24241 ccttctgtag aatatgaagt tgtagagatt agtggagagg tcttgcggat ttcctccccc
    24301 gctcgccttc cggatgaggc gctcattgcc gaggtccgtg aaccatggat gcgcctggaa
    24361 gtcatcagcc cgactgagtt ttatggcgtg atcatggaac tcaccaaaaa acgccgtggg
    24421 attttcgtct ctcaagatta cccttctccc aaccgcgtgc agctcaacta tgatatacca
    24481 ctatccgaat tgattgtaga tttcttcgat gaccttaagt cacgcacccg cggttatgct
    24541 tcaatggact accacttttt ggattaccga ccaggcaacc tggtgaaact cgagatcctg
    24601 gtggatagcg agcctgtcga cgcgctggca gcgattgttc ataaagaaga tgcctaccat
    24661 aaagggcagg cgctgatcac ccgcttgaag gcgctgattc cccgccagct tttcgatgtg
    24721 gcaatccagg cttctgccag cggacgggtg atttcacgcg cgaatgttaa agcgctgcgc
    24781 aaggatgtac tggcgaaatg ttatggcggg gatatcaccc gtaaaaagaa attgctcgaa
    24841 aaacagagaa aagggaaacg ccgcatgaaa atgattggtg atgtggaaat tccccaggaa
    24901 gcctttatgg ctgtgctgcg tttggaggat gaatgagcat ggtccgcccc aactggaaaa
    24961 agcaggctgc gaggtggctg ccggggctcc tcatcagcgc gatggcggtt tacgccatcg
    25021 cccatttcac ccgcgggcag cacattggcg aagccttaaa gacagtaaaa atcggctacg
    25081 ttttactgct catctttctc acgttattat cgctcatcgc gcgcagcatc gcctggaggg
    25141 caattctcgg caataaaatc accctggtca attcattttt tggtatttgc gaagggtatt
    25201 ttctcaacaa tatcttccct ttacgggtgg gagaactggg acgcgcgctt ttcgtcggtc
    25261 gttcttccgg gctgggaacg ctgcacgtgc tatcgaccat tcttatcgaa cgagcttttg
    25321 atattgtttt cgccgccagc attatattgc tcacattgcc catggttgtc ggttcagaat
    25381 ggatcaaacc gattgctttt tctgcctttg tggtcatcct ggttggcttg gtcatcctct
    25441 tcttaatttc agtaaagcgg cagaaatttc aaacctggat agaaagcaaa gaagtgcgca
    25501 ttggtttcat caagaaccgt gttttgccgc aagtcagtaa aatcctggat gggctttccg
    25561 ccctggtgca cccagggcag tttttgttaa gtgttttttg gattggcata acgtggttac
    25621 tgtgggtgct gctgtattac tggacgattg cgcaactggc accttccgcc ccgttatggt
    25681 ggggggcttt tacatcaggt ttgatcgcgc tgggtgttgc catcccatct gcgccggctt
    25741 cagtgggtgt ttatgaagcg accatcctgg gagccttcgc gctgctgggt atttccagcg
    25801 gttccgcggt ggcttatgcg attgtcttac atctggtgca gatttttgtc accgctgtgt
    25861 ttggtttgtg ggggatggcg cgggatggtc aaaaactatc cactttactt gattcgctgg
    25921 tgaacaaaaa aaacgtggaa aaccccacgg gttaggagag tgatgaacgg ttttacgctg
    25981 attacaggag gagcagggtt cattggtgcc aattatgcag ccaggtgcct ggctcgtggc
    26041 gaaaaagtgg tgatttttga taacctttca cgccgcggcg cagaattgaa tattgaatgg
    26101 ctgagcgacc gtttcgagcg gcgctcattt gaactggtca aagaagatgt gcgcgatgcc
    26161 gccgccatca aaacggctgc caggggagca gaccgcattc ttcacctggc tgggcaggtg
    26221 gcggtgacca cttcggtttg cgatccgcgc actgattttg agatcaatgc tggtggaaca
    26281 ttcaacgtgc ttgaagcagc acgcgcctca ggcaggaacc ctgtattcct gtacgcttcg
    26341 accaacaagg tgtatggggg gatggaggac gagcaggtta tcgagcagga aactcgcctg
    26401 gattatcttg accacccgca cggtatcaat gaaaaccaag cacttgattt tcattcgccc
    26461 tatggttgct ctaagggagc tggagatcaa tacacccgcg actattttcg aatttatggc
    26521 ctcccaaccg tcgttttcag gcaatcctgc atctatggtt cgcgccagtt tggtatcgaa
    26581 gaccagggct ggctggcatg gttcatcatc gcagcggtca ctggcaagcc gatcaccatt
    26641 tatggcaatg gtaaacaggt tcgcgatgtt ctgttcattg atgacctgct ggacgcttac
    26701 gatgccgcgt tttctcgcgt tgaggcgagc agggggcagg tctttaatat cggcggcggc
    26761 agggagaatt caatctcggt gtggcttgag ttccgcccaa tcctggagga attactcggt
    26821 cattctatca agacgcgaaa ccgtgaatgg cggccgggag atcagccggt ttacatctcg
    26881 gatatccgta aagcccggag cgtgctggga tgggaaccga gagttactgt aaaacagggg
    26941 atcaggcgat tatttgattg ggtaaaggag aaccagaacc tgttttgaag atcctgatcg
    27001 tcctcaccta ttatcgccct catatcagtg gtctgaccat ctatgcggag aggatcgcga
    27061 aagcctttgc ccgtcgcgga caccaggtga ctgttctaac ctcgcggttt gaaaaaaacc
    27121 tggctgcgga tgaattagac gaagatgtgc gcatcatccg cgcgccggtg ttgatgcgtt
    27181 tgagcaaggg tgtgatcatg cctacctttg gctggctggc cagcaaactg gtaaaagaac
    27241 atgatgtgat ccagttgcac ctgccacaat tcgatgcagc cggagttgcg ttacgcggca
    27301 ggctgctcaa aaagcccacc atcatcacct accattgtga tcttgaatta ccgcccggct
    27361 tatttaaccg ggcagccaac ctggcggtgt tgacgatgaa cgaactggct gccaaattta
    27421 ctcaccgtat cgtgacctat acagaagatt acgccaggca ctcttggtat acgcgtagat
    27481 atatgaagaa agtgaagatt atccctcctc cggtggtgtt gccgcgcgtt tcaaaaacag
    27541 agatcagcgc gtttcaggct gcgtataacc cgcaagccca acgaccggtt attggcatgg
    27601 ctgcgcgatt cgcaacagag aaaggcgtgg aagtgcttct gcgcgccatc ccccgtgtga
    27661 ttaacgaatt ccccaacgct ttgttccaat tcgttggacc ttataaaaat attattggag
    27721 aggaagggta tttcgcacgc ctggaacctc aaataaaaga atttgagcag gcaggtaact
    27781 ggaaattctt gggatcgatg tcgcccgcgc agatggcggc tttttacgcc aacctggatt
    27841 tgctggtagt tccgagcctg aactctactg aatcttttgg attggtgcag atcgaagcca
    27901 tgatgaacgg cgtacgcagc attacctctg accttcccgg ggtgcggcag ccggtgaaag
    27961 tgcatcgcat gggatccatt ttcccgattg gagatgctga tgcgcttgcc cgcggtatcc
    28021 tgcacgcttt acaaaacccg cagctcatac aggaagccaa cactgatttt tcggcttacg
    28081 accccgatac aaccgcagca gcgtatgaaa ggctttttaa cgaaatcacg gttgagttac
    28141 attaaaaccg ggcacaatca gcttcggaga gacaagtggt gactgaccag cctgaaaaga
    28201 aaaaacgagc caacaatgga atacgcggca acctggtttg ggacctgttg ctcattgtgg
    28261 tgctgctggc gggcgcaatc ttccgcttta ccggcattca atgggatggt aaccaacacc
    28321 ttcacccgga tgaacgtttt ctgaccatgg ttgagaccgg cattgcgccg gttaaaagcg
    28381 tctctgacta ttttgatacc gcaacttcca gcctgaaccc gaacaataga ggttatacct
    28441 tttatgttta tggcacgctg ccgttgttca ttgtgcgtta cgctggtgag tgggttggga
    28501 agtccggtta tgaccagatc aacgtggttg gcagggtgct ttccggggtt tttgacctgg
    28561 gcaccatcct ttttatatac ctgattgcca ggcggctgta ccgccacgcc cggctcgggt
    28621 tgctggcagc cgggtttagc gcgctggcgg tattgcagat tcagctctcg cattatttca
    28681 cagtggataa ttttgccaat tttttcacct atggcgctct acttactgcg gtgcatattg
    28741 ccacgtccag aaccttttcg cctgtcgata agcttaagga gaaaagatgg aaaaacgcgc
    28801 ctgactggct gacgactggc tggcgttcag cctggcttta cgcgcttttt ggtctgctgt
    28861 atgggatggc gctggcttct aaggtgagta tttgggcact ggcagccctt cttccgctgg
    28921 ctgcctggat ctattataag aatctccatg aggaggagaa ggcggctgct ctgccagtgt
    28981 tgattcgcaa cctggcactg gcaggggtga ttgcctttgt ttccttccgc gtttttcagc
    29041 cttacgcgtt tagcgggccg ggcttttttg gcttgaaatt gaaccccaac tggctgtcca
    29101 acatgcggga actttccacc ttaatgaaag gggatgtcga cgtgccttac gcccttcagt
    29161 gggcgcgccg accgctgacc tttaccattc agaaccttgt gttatggggg ctgggtgttc
    29221 cgctgggcgt actctcactg agcggcttgg tatggatgac ctggcgcatc atcaggggcg
    29281 attggaaaaa gcatattctc atctggtcat ggacgctgtt tgtactggtc acgcagtctt
    29341 tgaatttcgt gaaaagcatg cggtacatca tttccattta cccggctctg gcactgatcg
    29401 ccgcctggac gatctttaag ctgctggaaa agggcagcgc agttgggcga aaacggggat
    29461 tgggtcattt caactggggc cgaattcttt cttggcttac cctggtggtt accttgctgg
    29521 caaccacgct ttgggcggca gccttcagca gtatttacac aaaaccagtg accagggtcg
    29581 cagccagcga atggatctac gaaaatattc ccggagcgat caacctgaaa atcagcgggt
    29641 tagatgggca gatattcacc cagccgtacg cgtaccagaa cacagccgtc atttcttcag
    29701 aaaaccctta tcgctaccaa atcaccgcgg tgaagaatgg acaggtcaac agcgtcagtg
    29761 tgcagcacgt actgctgcat tccggttcca cgttgcaaaa tacgcttttg ctctcgatct
    29821 atgaatatgg tcagaacggt aagcatctgg caggagcagg ttttacgcaa gcggattttt
    29881 caggcctatc tgacaattcc ggtgaccagg cgctcatcca gctgcagacg ccggtgccac
    29941 tggaagctgg aaggcattac gaattggaat tttctgtggc ggagcctggc acctttttgc
    30001 gcatcggcgg cagcgtggca atcaacacca gccagtggag gatttatcta ccgcaaccgg
    30061 ttttccaact caccagcgct tctccgtatc agcttttgat ctaccctcga gaaaccggta
    30121 aactggatga gattgtccta aaccgagtgg cagaccaatc agaagagaac caggttacga
    30181 gggtcaaagc tttcatatat gccccggatg acccgcaaac cattttggcc agcgcggagc
    30241 tggtgagccg cttcgcgaac gaccctgacc tgcgtgggca gccttacacg ctgaaatttg
    30301 atgaagcgcc cttattagag aagaataaag gttattctct gctgttacag gtagaaggca
    30361 gcgcggttct gggggtttac aacagcatca ccgctctgga atccacctgg gatgacgctt
    30421 tgccggtggg tttatatggg tataaccagt tcggctatat cgatggaatt tatggtaatc
    30481 acctcaatat ggaattgtat tgggatgaca acgctgaaaa gctggaacgc ttaacttcga
    30541 tacttgacca ggcagacgcc ctcttcatca gctccaaccg ccaatgggga accatccctc
    30601 gagtaccaga gcgctatcca cttaccagcc agttctaccg ctcgctcatc ggctgccctg
    30661 agcaaaaaga catcttgtgg tgttacgccg tcgccaaacc ggggattttt cagggcgagc
    30721 ttgggtataa attgacggcg gtttttcaga atgaccccgc gcttgggccc tttaagatca
    30781 acgaccagct tgccgaagaa gcttttacag tttatgacca tcctaaggtg ttggtttttg
    30841 aaaaaacctc ggcctatgac ccggataaga ccgcgaaaac tctcaaaagc gtagacctga
    30901 ccagcgtggt ccataagact ccgcggcagg caagccgaat cagtggtaac ctgatgttga
    30961 ccgccaaaga agaggatatt cagacttccg gtggcacctg gagagaatta ttcccgccgg
    31021 aatcaccttt aaaccgacag ccatggctgg cggcggttac ctggtacgta gtggtcatgc
    31081 tgttgggctg gacggtttac ccgctggtga ggtttttctt tagagggctg ctggacaggg
    31141 gctatgggtt cagccggctg gttgggttgc tctttctcgc ttatcttacc tggatggcgg
    31201 gctcaatggg attgccttcc agtcgcgtca cgatcagcct ggtgtttttg cttttattga
    31261 gcacaagggt tgcagccgca gccctcagac ggggagagat ctggcaggag atacgaaaga
    31321 acagccgccg cttcctcatc attgaagggg tatttctggc tttattcgtg cttgacctgg
    31381 ccattcgtat aggtaaccct gatttatggc atccctggaa aggcggcgaa aaaccaatgg
    31441 acctgtcgta cttcactgcg gtccttaaaa gcaccacttt tccgccttac gatccctggt
    31501 attctggcgg ttacatcaat tattattact atggttttgt tattgttgcg gttttggtga
    31561 agtggctggg tattgaaccc gcgattgcct acaacctgat cctccccact cttttcgctt
    31621 ttaccggaat tggtgcatac actgttggct ggaacctttt tgccagatta aaactcgatg
    31681 atcgttcttc accgactgga gagaaacgca gctaccaggc gggcctggcg gcagcagcgg
    31741 cggtactgat tgccggcaac ctgggcacgc tgaagatgct gtggcagggc ctccagcgcc
    31801 tggcagcacc aggtggcatc atcgatggcg ccggattctt cgagaggctt ggctggttct
    31861 tttccggtgt gatgcggttc gtgaatggcg cgcggctgcc ttatgggttt ggggactggt
    31921 actggaaccc ttctcgagcc ataccgggtg atgttatcac cgagttccca ttctttacat
    31981 ttacttacgc tgatctgcac gcgcatatga tcgcgctgcc gatcacgctg cttgtgctgg
    32041 gttgggttgt ttcaatcttg tttcgcaact gggaggccca ttcttctgcc cgttttcaaa
    32101 ccgcggcagg tttgatcatc acgattcttt ttggcggatt tacgattgct acactgcgcc
    32161 ccacgaatac ctgggacctt cccacttatt tccttttagc ggtttttgtc cttttctaca
    32221 gttcttaccg gaacgcccaa cctcccagcg gttttctgcc agcagcccct tgctggttgc
    32281 gccgcattct ttattcaatg atttttatcg gcgtgttggc ggcgggggtg atgctttttt
    32341 atctgccttt cactgaaaaa ttcggccagg cttacggcat gatagatgta tggaaaggag
    32401 atcattctcc gttgggttct tacctggttc attggggttg gcagttgttc ctgattgtca
    32461 gctggtttgc ctgggagacg cgcgattggc tggcgtcgac gccggcttcc gccattcgcc
    32521 gcatgaagcc ctaccttggg tacctgcagg ttattggcgt gctttttacc atggtggtgg
    32581 ccttgctcac gctgctgcga gtgcatatcg gctggctggt atgcatactg ggcgtgtggg
    32641 cgctcgtgtt actgctgcgt cctcatcagg cggatagtaa acgccttgtt ctctttatga
    32701 ccggcaccgc gctggtttta accctgtttg tagagttgtt cgcgctccaa ggcgatatag
    32761 ggcgcatgaa cacagtattc aaattttact tccaagcctg gacgctgctg gccatcgccg
    32821 cttcggctgc gctgttgtgg ctgatccctg ctgcgacttc tgtgtggaaa tctggcatat
    32881 ccacagcctg gcaggttctc acggttattt tgatcgccgg ttctctcctt tacccgctga
    32941 ctgccagtat ggataaaata cgtgaccgta tgtctgcttc agcgccgatt ggtttggatg
    33001 gcgatgcatt catgcagagc gccacgtatt ttgaccaggg tgttgaaatg gacctcaatc
    33061 aggattacca ggcggttcaa tggatgagag aaaacgttca aggttctccg gtgatcattg
    33121 aaaccaatac agttgaatat cgctggggaa atcgtttcac gatttataca gggttgcccg
    33181 gtgtactggg ttggaactgg caccagcgcc agcagcgtgg ctttttggat tataacggga
    33241 ttaacaaccg cctgaacgat attccacttt tttatcagac gacggatatc gaacaggcgt
    33301 tggagattct taaacgctac caggtaaaat acatcattct gggtcaaatg gagcgcgcat
    33361 attacagcgg ggcagggttg gataaatttg caaactatga tggtctttac tggaaggaag
    33421 tatttcacga gaaagatacc gtcatttatg agatgattga ttaaatagga tagcgccagg
    33481 aatgtcacaa gccattaata tctttgtgtg gtacttggtg gggacctttg taggggtaat
    33541 cgcgctaccg ctggtattcc gatttttccc ctcgctaccg agcagaggat tcgcgctggc
    33601 tcgcccgctg ggattgttaa tctggggatt caccttctgg ttgcttgcat ctttcggcgt
    33661 gctgcaaaat gacctgggcg ggcaggtgct ggcggttctc attttgctgg cgttttcctt
    33721 ggcggagtta aagggcacgc gtaaagatgc actactcacc tggctgcggc agaacctgcg
    33781 aactgtcttg accattgaag ggctatttct gtttgttttt cttgcttggg tgctggtgcg
    33841 cgggatgaac ccagaagtgg cctacacaga gaaaccgatg gagctggctt ttattaactc
    33901 tattttacgc tccaccagtt ttccgccaca ggacccctgg ctctcaggtt acgctatctc
    33961 gtattactat tttggttatg tgatggtggc catgttgacg cgtttcagcg gggtggctgc
    34021 tgcaacagcg ttcaaccttg ccaatgcgag ctggctggca atgacagctg tggcattgta
    34081 cggcattgtg tttgacttat cgcttgctgg cgcctggaac agtcatcatc aaaccgcaaa
    34141 acaagttgaa aagttcgccc gcagagctgg ttttttaggg ccgttgtttg tcctcctgct
    34201 gagcacttat gaaggggtgc tggaaatttt gcacgccggc gggttgtttt ggaaggttga
    34261 tccccagggg caattgtctt cccgtttttg gaactggctt tccatccttg atctcaacca
    34321 gcccccgacg caaccattcg actggatacc ctcgcgtggc ggtggttggt tgtggtggcg
    34381 tggttcgcgt gtattgcaag atctaaacct gggtggacag catattgagg taatcgacga
    34441 atttcctttc ttttcgtatt tgctggccga tttgcatcca cacgtgctgg cgatgccttt
    34501 taccctgctg gcaatcgcgc tggcgatgaa tctgttcttt gatggaggga cctacctgca
    34561 acaaagccgc aggctgctgg attgggcccg gaacgagaac ttttggctga tcgcgttggt
    34621 actgggcagc ctggggttca tcaacacctg ggatttcccc atctatgttg gcttgttttg
    34681 cctgatctgg tcttttaaaa gctaccgcgc aacgggctgg aaaccaggtc ttttagccga
    34741 atttttgaaa aacgggcttg cgctgggagt ggccggaatg gtgctgtact tgcctttcta
    34801 ccttggtttc ggttcacagg cgggtggcat cctccccagc ctggagttta tgaccagagg
    34861 aattcacttc tgggtccttt ttgctgtgct tctcgtgccc atcatcgcat tttgcctgaa
    34921 ccaggcgaaa cgaaagctgg gatggagtga cttttggaaa ggcgccaggt ttacgctggc
    34981 gctgctctct gttcttttta tcttaatggt cttttacggc gcattgttac tgtcttttca
    35041 ggcgtgggga gcagggctgt cggcttcctc gaacctattc ctctccgcat tgggagtcaa
    35101 aatgacccag ggcggcgctg ccttcgccgg ggtgcacgcg gggtatccag cgggcgaggt
    35161 tctaaaagcc tcactgctca ggcgactgca ggcgcctggt acttggttga cgctggcggc
    35221 gctgttgacc attactttaa gcgttttgct taaacgaaaa acggctcaat cagttgaaat
    35281 tccagcagag ggagaacatt tgcgtatcac taatggcgca gaggagttga tcttttttct
    35341 gatcctgttt ggtttattgt taactatcat cccggaattt ttttaccttc gggaccagtt
    35401 tggtacccgc atgaatacca tctttaagtt ttattttcaa gcctgggtat tttggggcat
    35461 tgctgcggct tctggcagcg cagtgctttt tcgtcagttg cgcggatggc ggggtagcgt
    35521 tttccaaatt atgtggatct tggttgtggc gggcgggctg gcttacccgg taataatgct
    35581 gacggataag accaattcgt tctcgatgaa aggatggaca ttggatggta atgcgtacct
    35641 gcaaaaattc aaccctgaag aatacgcagc catcggctgg ttgcaagacg cgccgatggg
    35701 cgtggttgtc gaagcggtag gcggcagtta caccgattac gcgcggatat ccacgcgcac
    35761 cggcttaccc actctattgg gctggcctgg gcatgaaagc cagtggcgcg gcggcgggga
    35821 ggaaatgggc tccaggtttg cagatatcca acagttgtac gaaacgacgg attggaatga
    35881 ggtttggcgc acgctgcgag actataatgt gaggtatgtt tatatcggca accttgaacg
    35941 ctcaacctac cgggtgtcac catcgaaatt cgatagtcat ttgccgcttg ttttctccaa
    36001 tagcagcacg aggatatacg aggtcactgc cccctctgga gaagcgcagc catgaacggt
    36061 aatcttgatt cacgaaaagc tgtaccgctt gagttgctgg tttacctggt agtgctggtt
    36121 attgccgcag cgctacgttt ttcaaacctg ggtggtttac ctctgactga tcatgaagcc
    36181 agcctggcct tgcaggcgct gcgtttgagc aaaggagagc aggtgctgat ctctggcgag
    36241 ccaggttaca tcgcattgac cactttgtgg ttttctctat ttgaactctc tgattttacc
    36301 gcgaggttct ggccagcgtt ggcgggcaca ctgcttgccc tcacaccgct gctctaccgg
    36361 gagagggtgg ggaaagccgc cgcgctggtg ctggctggcc ttgtggcggt tgaccccata
    36421 atgatcggcg catctcgcag cgctgaaggg agtatgctgg ctttattagg gttgctcgca
    36481 gcagtgggtt ttgccatgcg ctccaagccg ctttggtgcg ggttcgcctt gggattcgcc
    36541 ctggcaggag gacctgcgct gtggccagga gtaatctgcc tggcggtttt cctgttgatc
    36601 aactgcaaga gccttcgcca ggttgacaac cgttttttgg cgctggcagc aatgaccgcg
    36661 atggtcaccc tggtcttatt gagcactctc ttcttgatca acccgcccgg aatcagcgct
    36721 tttgggtcga gcctcgtgga ttactggggc tcatggagaa gtgagaaccc cgcgcccctg
    36781 aaagggattg cggctgcctg gggatcgagc acgctgctcg tgctggtgct gtctatttgg
    36841 agcctgatac agggcttcgt tcgcggcgaa gcccgtgcca aatggctggg aatatgggct
    36901 ggtgcagccc tggcactgat attgatgaac cctgcaagac aattattcga tttttcctgg
    36961 gcaactgtgc cattacttgc cctggcagcg atgaaactag tggagttgtt cccatctttt
    37021 tcatctgata accgcattgt tttcatcgcg gaaacagttt tggtggtcgc actggtggtt
    37081 ttttcgttta tgaacgcggt caacctggtc aataaccctt tgcaaagccc ggaggaatac
    37141 cgcaaccgag cgattggcgc ggtgctgccc ttgttcctgt tactgggcat gactgtgctg
    37201 ctcgcctggg gctggtctgg ggcatccacc cgggagggtt tcattgcggg cgtgctgttg
    37261 ctggctggct ttttcaccct ggcaaattcc attaaagccg cctcattggg cagccgacca
    37321 gaaattgaaa taagaaggga cctggcggct gttgtgggct cagaagaact gctgcgcaca
    37381 atccaagaca tctcgcgggc gagaacaggg atcgaaaacc gcattgatgt cttgatagtt
    37441 gacttgcaga gtccctcgct tgaatgggca ttgcgcagtt ttgaaaaagc cgaaaccgcg
    37501 gctgtctttg acccgaccgc atcaccctct ctcgtgatta cagacgttaa ccaacaaatc
    37561 accgctgggg cgtcttatcg cggtcaaaaa attatttggc aggaagagcc tgacatgaag
    37621 aatatgacga tcatagactg gttgaaatgg tcatttttcc gtatcgcacc tcttgagaat
    37681 agagaaatca taatctgggc gcgcaacgat cttttcccgg gagggaacat accatgagta
    37741 caaggggtgc tgccatgcgc aaacagcaga aacctttggt catcgccatc gatggacccg
    37801 ccgcttcggg caaatccacg ctggcagaaa agctcgccca gcggcttggt tttttttatt
    37861 ttgataccgg tgttatgtat cgcgcggcaa cgctcgcagc gatgaaggac ctgggcggtg
    37921 tggaggatga gaaagccgtc accaggctga cagcggcggt tcaaatcgat gttcgcccgc
    37981 catctgttga agatggcagg gtgatggatg tgctgctgaa cggcgaagat gtcacctggg
    38041 agattcgcac tcccgaggtc gaccgcaacg tttcgccagt ctctgcttat gagggagtgc
    38101 ggacaattct cacaaatcgc caaagagaca tcggcatgcg gggaaacatg gtgatggtgg
    38161 gacgggacat tggcacggtg gtgttccctg acgcgcgata taaaatctac ctggaagcct
    38221 cgccggagga aagagccagg cggcgactga aagagatgca gctgcggggt gaaacccatt
    38281 cttttgaaga ggtgctgcaa tccattatca ggcgggatgg cattgactca acccggcaac
    38341 acgctccgct taaaccagca gttgacgcgg taatcatcga cacagatggc aagtcgattg
    38401 aagaagtgct cgaagaaacg cttgccatcg ttcaaccatg aatttatact gattgttgat
    38461 ttcgttacaa cgagaaggag gattccatgt gcgataccat tgtggctttg ggtaattcca
    38521 ctgtggacgg taatgttatt tttggcaaaa actcagaccg gcaccctaat gaagcgcacg
    38581 aggtggtgat ctttcccgct gcgcagcacc caaccggcac cactgtcaaa tgcacataca
    38641 ttgaaatccc acaggttccc gcaacacacc aggtgctgtt ggcaaagcct ttctggatct
    38701 ggggcgcgga gatgggcagc aatgaacatg gtgtaaccat cggtaacgag gcggttttta
    38761 cgcgcacccc ctacggcaga gaacccgggc tcataggaat ggatttgtta cggctggctt
    38821 tggaacgcac ggatagtgct gaagccgcaa tgatgctgat cattcatcta ttggaagaat
    38881 acgggcaggg aggcaactgc ggttttggac acaaaatgta ttatcacaac agttttctga
    38941 tttgcgaccc aaaagaagcc tgggtgcttg agacttcagg caaagaatgg gcggcagaaa
    39001 aggtcagaga tgtgcgcagt atttcaaacg ccatcaccat tggcgaaaaa tgggatctat
    39061 gttcggcgaa tctggtcagt agcgcgatta agagaggttg gtgccgttct gaatcggagt
    39121 ttcattttgc cagatgttac tctgagccaa tctataccac gttcgcagcc gggcgcagcc
    39181 ggcaagcctg cacggctgac agcctgaggg cacagaaagg aaacatcacc gcgacaagta
    39241 tgatgcgggt tctacgcgcg cacacgcggg aaataaaacc cacctggtca ccagaccgcg
    39301 ggattaccgg agcggatgtg tgcatgcacg cggggtgggg accgatacga gaaaaccaga
    39361 ccgcgggctc catggtgtct gtgctgcgca gcggaggtcg ttctgaacat tgggtgaccg
    39421 ccacagccgt gccatgcacg tcgctgttca aaccagtatg gctggacgcg ggagtgccct
    39481 ctactggaaa tgcccctact ggcgaatatg atcacgcggc gctttactgg cgtcacgaag
    39541 cgctgcagcg ggaaatcgcc agggattacc aggcgcgcat cgccgtgatc aaagacgcac
    39601 aggcgacctt tgaaaatgaa ttaacagctg aggttgccag agcggataac gccacagcgc
    39661 agcagcggcg gctgatttca gaaaatgctt ttagacgggc ggatgaaatt gaagaagact
    39721 ggtatgccca aatccagggc atgaaaatta cccggcagaa caccttgctt taccggtctg
    39781 cctggaataa attcaatgct caagcgaaaa tgaaataacg ttgtatcata atagtagatg
    39841 atcagaattc acctcttcaa cgcggggccg tttttcgata cgccttacca gttggtaggc
    39901 tggattggtt ggtttgtcat ggcggcgttg attctatggg ttgcgcggaa agacctgacc
    39961 cgacagcgca cgagccttat ttggaagtac tttttcgccc tggggataat ggcgatcgtt
    40021 agtactttgt tttttggttt tgaattacca ccttacagca aggttccgtt accgaatatc
    40081 ccgggagaga tacaaccgcc cgccgtgatg gtgctgatga tggtgcccat catgctggca
    40141 gcgggcatat tgggtacctg gccggcagcc gttttaggtt ttatcgcggg aatttttagc
    40201 gcgttgtgga atacccactc tgtctttaca cccctggaaa tttctgttct ggctgtggta
    40261 atcagcctcg ctttgcgcca gaattatcgc acgcggttct tcgctttttt acgcagaccg
    40321 attggggctg ccctgcttgc ggctttcttt tccatcccgc tgttcctggt cagcactttc
    40381 tttggcacaa atggcaacct ggcggcaaaa ctcgattacg ccttcactcg ctcgtgggtg
    40441 ctgattggcg tttctacgat acagttgctc atcgccgcgc ttatttgcga ggtttttcgc
    40501 gcgcccaatt caagatattg ggttaagttc ggctcgtttg aaccttcacc agtagagacg
    40561 gggttacaga gccgcattct ttacacagcc ctgccgctgg tttggatact gctgacgacg
    40621 ctggtcatcg cggattggtt tgtcgctggc cgggcggcgc gtgatattct ggtgaaacaa
    40681 cttcaagata cttccaaaat cgctgtggag aatatcccgc caattttgga gaccggtcaa
    40741 agcctgacgg cggcgctggc tacccagaaa ctgcccttag gtgatacaca acagctcaga
    40801 gatttattgc agagtaaagt acgggaattc ccattctttt cccaaatata catttttaac
    40861 ctgacgggtt ctccgctgac tggttaccca gatgacgaag ttggcgaatt tcgtttatcc
    40921 tctgaagagg agctgggaat ccaactggcg ttgaacgggg tgcaaatgca gaactatgtg
    40981 atccacccct atcccggtgc ggaatccgcg gtaatctcgt atatcgctgc aattcctgat
    41041 gaatacggtt tagcccaagg cgtggttatt acccgtacag acctgacgat gaacctgttt
    41101 tcacagccag cgctgattgc cttgcagaac ctggttgaag aaggtggtca gggcgtgatc
    41161 atagacgaaa caaatcgcgt tctgtaccac accaacccca agttgattat gaccacctat
    41221 accgggcaga ttcccgctgt ttcggggtat tttgaagacc ccgggctcac gggaacccgc
    41281 tatatgaact atgcgtctgt ggatgactta aatggctgga aagtcattct ctcggtgccc
    41341 gcaaatcatt ctcagcaatt ggcgctgcga atagctattc cgttgctggt catctccctc
    41401 attatttctg ttttggcgta tttattattg cgttacctga taaaaacact cactcagtcc
    41461 ctcgttcttt tagccaataa agcggatgaa atttccaaag gcgagctgga taaccgcgtc
    41521 gaggtaaagg gagtggatga aattggccgg ttgagctccg cttttgagca gatgcgcctc
    41581 agcctgaaat tgcgcttgga ggagctggac gcgctgctga aggtttctca aggaatcgct
    41641 tctgacctct cattggaatc aacttcggcg catctattga gtgccttgcg atcgtatggg
    41701 gcggatgccg ccagtttggt gcttacccgg ggaacgaagg agggctggga cgaagggttg
    41761 gaagcctatc gatcgggtga acaggccgag gaatacgcct acctcgatca cttattgctg
    41821 gaacatttac aggaagagtc cctattgatc atcccatcca gagccagaat ccggcgcatg
    41881 gctttaccca aaacagcacc cattccttct gctttagctg ccatcgcctt gaacaaaagt
    41941 ggtgaaaacc ctggatttat ttgggttgtt tacacccaat cgcatcgttt cctggaacct
    42001 gagattcgtt ttctggatac cctggcgggg caggcactgt tagctgtatc taactcgctg
    42061 ctttaccaga aagcggaagt aggcaaaaaa cggctggagt ctgtgttatc ttcaacccct
    42121 gaaccggtgc tggtagccgg tgaagacgga cggctaatga tggtaaacca ggcagcggaa
    42181 gaaatgaccc tgttaataca attgaacgct caaggtactg aaagtcgcgg cgagatcatc
    42241 tccaggctgt taaaagaatt catcaccggc accaatccca cggagagccg ggcagaggaa
    42301 atggaacttg aagaccatca tatttacctg gtttcagtat caccggtagc ggtggaaagc
    42361 aagcacctgg gaaaggtctg cgttttgaga gatgtcaccg attacaaaca attggagaag
    42421 atgaaatcag agtatgtttc taccgtcagc catgatttga aagccccgtt agtattgatt
    42481 cggggttatg catcgatgct gccgatggta ggcgatttaa atgaacaaca acgagattac
    42541 tcacaaaaaa tcctggacgg catcgatgat atctccagga tggccgacaa ccttctggac
    42601 atgcgcagga tagattctgg tatccagctg caggtggaaa aggtgtcgcc ctcagacctg
    42661 cttgatgaag tgattgaaga gatgcagccg cagatcaaac accgcaagat acagattctt
    42721 cgtgaactga cgcttgcgca aaacctgacc attgaagccg acagggtact cttgcagcgc
    42781 gccttacata acctgttaga taacgcggtg aagttttctt cattaggaag ccaggtgaac
    42841 ctgcgcttgc aggtaaatga aaatagcgtg gtttttgaga ttcaggatca cggctcgggg
    42901 attgcgccgt tggatcagcc ccatgtattt gaaagtatca gaaggaatac agatgggagc
    42961 ccgaagaagg aatggggagt gggactgtcg atcgtcaggt ccatcgcgga gcgccaccag
    43021 ggaagggtat ggcttgaaag tcaattgggg aaaggcagca cattctacct ggaaatccct
    43081 ttacagcatc aggcggcaga aacaaataaa tagtaaagaa atctgaaatt tgtggtaaaa
    43141 taccagattg catgtataca atggcggaat gttgcctgtt cgtggtcgta ggcaaaattc
    43201 tgattggaca atttcgtttt tttaactgct gcaggtcttt ctcattcagg aggcattagt
    43261 ggaaacaaaa agtttagtcg cgctccgtat caatctcgca tcccctgaaa caattcgaag
    43321 ctggtcttat ggtgaggtgc tcaaacctga aacgatcaat tatcgccggt taagaccaga
    43381 gaaagatggt cttttttgtg aggcgatctt tggaccttcg cgagattatc agtgttattg
    43441 cggtaagtac aaaaatccac gttacaaagg tattgtttgt gataaatgcg gcgttgaagt
    43501 tactcgttct tcagttcgcc gtgaacgcat ggggcatatt gacctggcga caccggtcgc
    43561 tcatatctgg tatacccgca ggatcccctc ttacctgggt ctgctattgg atatttcccg
    43621 gcgtaatttg gaccgcgtgc tttatttcgc acagtacatt gtcacatttg ttgatgatga
    43681 tgccaggcag aaggcgctgc gcaggctgat ggatgaaatc aatgattccg agctggaaca
    43741 ggctaaccgc atcaacgccc agatcctgga agtgaagttg ggtcgagaca agaaggtggc
    43801 tgaattacag agccgcttga aagaaattga atcgaaagcc gaagaacaaa ttacttcaaa
    43861 gctcgaaccg gtcattcagg aggggcaatc tcttgaacga cagcttaccg agctttcagg
    43921 aaaagccgcc aaaaagaaga tcttctttga gactgcagac gtcatgatcg cggagaaagg
    43981 cgagattatt gctccttcac acatcgccag tgtgcagaaa gcggtgaagg aacgcctgga
    44041 agagatagag gacgaataca aaaaacaatt ggacatggac cgcgaacagc ttaaaatggc
    44101 cattgaacag gtcaaagcag aagccgaagt ttccatggat gaactgcgca accagctgga
    44161 ggatcaatca actgaatctc acgatctttc tggcagaatg cgcgatgaac tggaagagct
    44221 tcgccctttc accttcctca ctgaaagccg ctaccgcgaa ctgaaatccc gatggggaca
    44281 ggtattcagg gctgatatgg gcgctgaagc gttttatgat atcctgcgcc gcatcgacct
    44341 ggataagctg tcagaagatc tgtggactga agttcgcacc acgaagagca aacaaaaacg
    44401 aaaaaaggct accacacgac tcaaagtaat cgagtccttc cgccgctcag gtaaccgccc
    44461 tgagtggatg atcttaactg tgctcccggt cattccgcct gacttgcgcc cgatggtgcc
    44521 gctcgatggt gggcggtttg ccacttctga tatgaatgac ctgtatcggc gggtgatcaa
    44581 ccgcaataac cgcctgaaga ggttgttaga acttggcgct ccagatgtca ttgtgcgtaa
    44641 tgaaaaacgt atgctccagg aagcggtcga ctcgctcatt gacaactctc agcgcggcaa
    44701 ggcgctctcc cgccgcggac ggcgtgaatt gaagtctctc agtgatatgc tcaagggcaa
    44761 gaaagggcgt ttccgccgca acctgcttgg caagcgcgtg gattattctg gccgctcggt
    44821 gattgttgtt ggaccaaaac tgaagctata ccagtgcggt ctgccaaaaa cgatggcgct
    44881 tgagctgttc agacccttcg tagtagcacg ccttgtgcag cactcctacg cggcgaacgt
    44941 gaaatccgcc cggcgtttga tcgaacgcaa cagacctgag gtgtatgaag tgctggaaga
    45001 ggtcatcaaa gaaagacccg tgatgttgaa ccgcgcgcca acgctgcacc gcctgggcat
    45061 tcaggctttc gaacctattc taatcgaagg cagcgccata caacttcacc cgttggtgac
    45121 aacggctttc aacgccgact ttgacggtga ccagatggct gtgcacgtac cgctttcaga
    45181 caaagcggtt caagaagcgc ggaacttgat gctttcttca aagaacttgc tcaaaccagc
    45241 cgatggcgaa ccgatcatca gcccggcgaa ggacatggtg ctgggtattt actaccttac
    45301 cgttcaggat aaccgcaaac acaaaggcga tggccgggtc ttcgcagata tggatgaagt
    45361 tgagctggcc tacaaccttg gtcaggtaga ccttcatgcc aatataaaga ttcgcatgaa
    45421 cacctggttc aacgctgaaa acaaacgcat gcccaaagct gaaaccagaa tgatagatac
    45481 cacggttggg cgtgtgctct tcaaccggat tctgcctgaa gaggttcaat tcatcaattt
    45541 cgtgcttgaa aaatctgggg taaaagatct catcgccgat gtttatgacc tgtgcggaga
    45601 atcggtcaca actgaagtgg ctgacgctat taaagatatt ggctttgagt acgctatgaa
    45661 atccggcaca acgatcgcgg tcgcggatat taccattcct gaagcaaagt atgaaattat
    45721 tggctcagcc cagcaggaaa ttgaccgcat caaccgtgac tatcgccgtg gtctattgaa
    45781 agaagaagag cgtgacaaac tggtcatcga catctggaaa aaaacgactg aagatgtagg
    45841 agcggccgtg cgcgcgggga tggatcccaa cggaaatctt tccgtaatgg cctcttctgg
    45901 cgccaccaaa ggcggttttg gtcctatttc gcagctggct ggcatgcgcg gcctgatggc
    45961 ggaccccgcc gggcgaatta tccgcctgcc gattcaatct aatttccgtg aaggattgac
    46021 ggcgcttgaa tacttcattt ccactcacgg cgcgcgtaaa gggttggcag ataccgcctt
    46081 gcgcacggct gacgctggtt atttgacccg ccgcctggtg gatattgctc aggatatcat
    46141 tattaacgag gaagactgcg gaactgaaag tggcatctgg atccgcggcg cagatgatgt
    46201 ggctggacaa aaattaaaag aaagattgta cggtcgaacg gtcgcgggtc gaattgtgaa
    46261 ccccaagacg ggcgaaatca tcgcggaacg aggggacatt ctggaccatg acctgatccg
    46321 caaggttacc gacgccggcg tagaagatgt gtatgtgcgt tcccctctga cctgcgagct
    46381 gatccacggt atttgtgcca agtgctatgg catggacctg gggcgcggtt ctctggtgac
    46441 actgggctct gctgtgggca cagttgccgc ccaatccatt ggagagcctg gcacgcagtt
    46501 gacgctgcgc accttccacc tgggcggtgt ggcttcggct ggagacatca ctactggtct
    46561 gccgcgcgtg gaagaacttt tcgaggcgcg ccgggccccg aaaggtgaag cggtgatcac
    46621 tgcaatttca gggattgcta aagtgttgca ggcagatcgc gttggtgacc agaagctcgt
    46681 gcgcgtggaa cacagcgaac tggtgtcgga tgagtatgaa attcccaccg attgggagat
    46741 actggcgaac gatgccagcg agattgtcac cggtgatccg gtcgccagaa tgggcgatgc
    46801 cttgatcaac gcccaacatg acggaagggt gcgcgttgaa ggtcgtaagg tgattgtttc
    46861 atatgagcag aaagagagcg aagattacga gattcccgga acttcgcgca tgattatccg
    46921 cgacggtgac caggttgaag cgggacaggc gttgacggaa gggtccatca atcctcatac
    46981 catcctcaag atcaaggggc gcgaggcttc acaaatgtac ctgttgaacg agattcaaaa
    47041 ggtatatcga gcgcaagggc aaaacatcaa tgacaagcac tttgaagtga tcattcgtaa
    47101 gatgattgga aaggtgcagg tcatcaggcc tggcgacacc aactacctac cgatggatgt
    47161 ggtggaccga ttggagataa agcgcgtcaa tgaagaaatg attgcccagg gtaaacagcc
    47221 cgcccgttat gccgagattt tgctgggagt gaccaaagcg tcgctgagca ctgattcgtt
    47281 cctttcagcc gcttcgttcc agcataccat caaagtgctt gctggagcgg ctatcgcctc
    47341 ttccgaagac ccgctgtttg gcttgaaaga aaatgtcatc attggcaagc tcatccccgc
    47401 tggcaccggt tttgtctacg gtcggtttat cccagcggtt gaaggcgaaa ccgcagaggt
    47461 cgaggttgct gctgaacctg aaaccgaaac gaaaattgaa tagacttgaa taatcattta
    47521 ccaagaacac tccggattaa aatatatccc cggagtgttt ttttaatgaa agcctgatag
    47581 ggctctttag ccgcaatctt tttattgtaa acttatctta tccaataaaa aaccgccgga
    47641 gattagatta tggatatggg ttctgttcgc aagattgata ttgatcacga aatgcagcaa
    47701 tcatacctgg attacgccat gagcgttatc gtatctcgcg cattgcctga tgccagggat
    47761 ggattgaaac ctgtgcagcg gcgattgctg tatgccatgt acgacatggg cgtcagggct
    47821 gactctgcat ataaaaaatc ggcgcgtatt gttggtgaag tattggggaa ataccacccg
    47881 catggagacc aggcggttta tgaggcgatg gcccgtctgg cgcaggattt ttcaatgcgc
    47941 tgcctgctcg tggatggaca gggaaatttc ggcagcgtgg atggggaccc tcctgcagcc
    48001 atgcgttaca ccgaggcaag gctgacatcg atcgcgatgg aattgctcag tcagatcgat
    48061 cgagatacgg ttgattttgt gcgcaacttt gatgactctc tggatgaacc actggtgctg
    48121 ccgtctgcca tcccgaacat gctggtgaat ggcgcttcag gcatagcagt gggtatggct
    48181 accagcatac ctccacacaa cctggttgaa gtgattgacg gcctggcttt cttgctggat
    48241 aactggcaaa agatggaaga tattaccgtt accgacctga tgaaattcat caaaggacct
    48301 gattttccca cgggcggcat cattttgcag gagcatgaac aggacgagat ccttgcggct
    48361 tacgccacgg gcaagggcag aatcacgctg cgaggcaggt tgaacctgga ggaaatgtcc
    48421 cgcggaaaga gccgcattat catcagcgaa ctgccctata tgaccaacaa atctgccttg
    48481 attgagagga tcgcggagct cacccgtgaa gggttgctgg aaggcatttc tgatctgcgt
    48541 gatgaatcag accggcaggg aatgcggatt gtcatcgaat taaacaaggc ggcggatgct
    48601 gaagccgtgc tgcgagagtt gtataagcgc actccgctgc aggtgacctt cagcatcaac
    48661 ctgcttgccc tggtcaataa cgagcccagg ctcttacctt taaaacaagc cttgcgggtg
    48721 tatcttgagc atcgtattga ggtggttaaa cggcgcagtg aatttgacct caggaaggca
    48781 caagaaagga ttcatatcct ggaaggactg aggatcgcgt tgcagaacct ggatgaagtg
    48841 atcgccatta taaaaaaatc cactgatacg gatgacgcgc gcgagcgttt gattaaaaaa
    48901 tacaaactta ccttgatcca ggcgaacgcg atcctggaca tgcccttgaa acgcctggct
    48961 cagctggaaa gaaaaaaaat cgaacaggaa taccgcgagc tggtgcagtt gatcaaagag
    49021 ctactggcgc tgctgcgttc accggcgaag caacgacagg tggtgggtga tgaactgaaa
    49081 gagatccgca cgaagtacgc tgaccggcga aaaacacaga tcgttaacct gaagaaaggc
    49141 aaggctgctg cagaccttct gacggtgaag gatgttaccc cggttgaatc cgtttgggtg
    49201 gtaatggatg aacaggggag aatcggaaaa ataaacgcgg aaaaacctcc gcgcatgagc
    49261 ggcagagacg cgccggtctt cgccatgcgt actgattctc accagacgtt gtacctggtt
    49321 ggacaggacg gtcaggctgc ggctgtctcg gtccagagtg ttcccgagat tgtcaaccca
    49381 ggagaaggca gccattattc cagcatttca ccgttgcgaa ccacgaacct tcccgcgtct
    49441 gtgttctgcc tgcctgctgc tgatttgcag gaactgtttg tgctcaccat cacccggcag
    49501 gggttgataa aaaagacact cgcgactgag ctgaaagggc cgtcttcaga cctgttcact
    49561 ttggtaaagg tgaatgaagg agatgaactc gctgatgtgc tgttggtaac tgaaaaagcg
    49621 cattacctgg tcgtcacccg ttcaggaatg gcaattcgct ttgaaggcag tgaggtgaga
    49681 ccgatgggac tgcttgccgc gggagtcaac gctatcaagc tgagcgctgg tgatgaggtg
    49741 gcgggaatga tagaaattgc ccgcaaagga gacgtggtac tggtagccag taacggggca
    49801 ggttggcgcg ttgagccaga tggtttcccg cttcagggtc gttatgggca gggagtgatt
    49861 ggctgccgta tggctgatgg ggtggggttg attggtgtgg tgtttggtaa aaagaacttc
    49921 caatataccc ttcacctgaa gaaagccgca gccaaaacgt gcagagtgga tgatatcggc
    49981 tacaccaaac gcgcgggtaa aagtgagcag atcctgcagc tggcttctgg agaccgggta
    50041 gaaagaattg tacggatgat ggattctgcg gaatgagcgt tcataccgcg tattactcaa
    50101 ttgagtagta gcagtaatgg tataacaccc tgccgtcttt taaaccgacg gtgtcttttc
    50161 cattattaac gatgcctctt cgagagtggc aggtccatgt atagtggatg acgttgtctt
    50221 ttacctcttt ggaggtgact ctgaaatcac catcattcaa ctggttggta agcatctggc
    50281 caaagaaacc ccggatattg gcattcccct ggatggtctg ctggggggta accaacacag
    50341 cgttgggggc gtaaatttcc aacatggggt catattgcat ggcgtttaac gcgtcgaaga
    50401 gacgagtaac cgggtcggag gacggcggtg gaacgctccc ctgcgccggc caggaatacc
    50461 tggcaatctc gtcccataca ggcacgagat gcacccggga ataatcccac gagtagaaat
    50521 tcgctgccgt caaccccaaa gaccgggcgg tatcaagaaa tagttttacc tccgacggag
    50581 aggggtacca tccgctttca ccatacgccg ggcccgtggg cacaataggg cggaagggag
    50641 atatggcttt aaattcatta acgctgcgtt tgagatcata atccgggttg tgtgcttgca
    50701 tccaataaac ctgcggcatg ttcaaatcgc atttttcaag gaaggcggaa tacgggaaag
    50761 agcggtgata ggttggccat ctaaaagaac tcagcgctac cggcgttgaa ggaatacctt
    50821 tacgcaactg cgtcatgaaa attttcgcaa cagcttcgcg cccggcttgc ttatattcca
    50881 cctccgcatc aatcacataa ccatctaagg cgtattttcg ggtctgcgag acggcaatat
    50941 tggcttcacc aactgggtca tatccgtaaa cataatgcca gccccaaacc tggatgcctt
    51001 tggcgcgcaa cgcatcgacc accggcggga tcaagtctct accgctgcta tcgacgttgt
    51061 atgggcgggt atcattcgct atcttaatga ggacgtgtga aaacccagaa gaggctgcca
    51121 cccgggcgat agctgatgga ttgccggctt cgcagctggg gattttccat atcatcatac
    51181 ctttcccggt caaggtcatc gatctctcct ctgatcagcc aggtgatgaa cctggctcca
    51241 ggttaccagc aagcccgatt tgcgtggcga tggcgcgcat ggcgatgatc acattctcgc
    51301 aatgctccca caacgggaca ccaaattctt cagcgccgcg agcaatctct tcgcggttgg
    51361 ctcctgcggc gaaagcctta tccttccact tcttcttgac tgaactggct tcaagatcat
    51421 aaagtgagcg agaagggcgc accagggcaa ccgcagtgat caggccagta atttcatcgc
    51481 atgcgtataa gcccttcgct agcaaactgg tgcgagggat gttgaggttt tggccatggg
    51541 cgagaattgc ttggattacc tcttctgcta caccgtgttg acgcagtatc ggcgccccct
    51601 ccaacggatg ttgctcagcg gatgggtgaa tttcccaatc aaaatcgtgc agcaagccag
    51661 ttacagacca ggtttctacc ggctgcccca atttttcggc gtaaaaacac atagccgctt
    51721 ccaccgccag catgtgatgg acgaggtttt cattttttac atattgtctg acgagtttta
    51781 acgcttcttc acgattcata ttttcagtgg ttatcggact gctcagcaaa aatgggctcg
    51841 ggttttccca ggacgggtaa aggtttggaa acccatacct gccataacgc gaccagcgtg
    51901 gcaggcacct cgcgaatacg ccaaaaacgc tccgcgtggt catgataatc acgcaggtca
    51961 gcgattacac catttacttt catgcccatc ccgctgcagg taaaaatggc tcgagggaga
    52021 tggaactttt gggttacaac cagaatatca tccagaccga agatgtctct ggcgcgataa
    52081 catgtgtcgt aggtgcgccg gccggcaaaa tccagcacaa tggcttcttc cggcactccc
    52141 tggctgagcg cgtagtcgcg cattgaagcc ggctcattgt aatcgacaaa gcgattatcg
    52201 ccactcatca ataatttttt cacctttccc gcctggtaaa gctgcacggc agcggtcacc
    52261 cggtctttaa gcactgggga gggagtaccg tccttttgca aaccggcccc aaacacaaca
    52321 gccaccggcg ctgagggggc gtcagctacg ctgtatattc tgccggcagc agaaagtgag
    52381 gttaccacct tcggaagccc gaagagcacc agtaacagga ggattgaaaa aagcaccagt
    52441 tttaaaagca tttttattat ccttgaaaac atagcaagaa ttgtaccagt aaagcagcca
    52501 gttctaagac gggccgatcg atcctgatga aaggtctaaa ttcgaaatgt ccactgcgcg
    52561 gatccgtatt ttgcgcttac caattcagcg gccattttaa gctcttgttc gctgggctgg
    52621 ctggaggaga aatgtatatt gcccgcctta ctgaaaccag ttataacggc ctccgcgaca
    52681 gtctccatgc tcggcgcggt tcccattacc gcttccagtg tggaagcgcg ggcgatcagc
    52741 ctgttggcgg ctgtttgacg cgcttgttcg gaagggaatt ggagtacccg ggtaatgcgg
    52801 gcgaggtcac ctttgattgg tattgaacca tgttgcagaa taccgccgta ctttcttgac
    52861 tgagcagaac cgatcaattt ttttccatca accgtgatct cataattgga aggtgtttca
    52921 aagcaaaccg ggtttttctt attgtccggc ggttcattgg tgtaggcttt atctccgaag
    52981 gctgtcacat tcatgcgggc caaaccggtc agtaaagccc ttgaaatttt gcagtaactt
    53041 tccagcaggc cacccgccag gaagggctca tcatcgctgc ctgccacagc gtaagtgagc
    53101 tcatccacat gcaatatcgc ccggccgcca gttggtctgc gcaccagccc ccattgttct
    53161 tcttctaagg cagcaagatc aacatcaagg tgaggttgag caaatcccag tgatagagtc
    53221 gctggttgcc aagcatacat cctgagggtt ggaggtgagt ttttttgcgc tgtggaaaat
    53281 aagatggctt catccaagcc catattccag gctccggatg cgggaggaga aaggatgagc
    53341 cgccattctc ttaacatatc tgggtaattt tactacgagt aatcagtgcg gcggtgagcg
    53401 taatctgtct aaatagatat gaattatctt tatttacttg acaaaaatga taggaacggc
    53461 taatattaaa ccgctttgaa aaacaatatg gaaaatctcg tcttaatggt gggtgagcga
    53521 ctgggagttc cgcaaatcag agagaacctc cgaagtatga atttttcact tgccatcacc
    53581 ctgaagggtg atagatagat tccacatgca acaaggaggt ggttgacacc ccacgtttac
    53641 cttaaagcga caacatgaat gtctactatg ttcaaaactt aaaaaagagg agaaaaaatg
    53701 acaaaaaagt tgttccccct gtttagcgta ctgcttgtgg cagtcatgct gttggccgcc
    53761 tgcggaccga aagaagcagg ccctgtaacc atcaacctct ggacgaaaga aggtgaggca
    53821 gatggtggtt tgcagtatgt gcaggcgctt gccgatgcct atactgctgc gcaccccaat
    53881 gtgacctttg tggtgacgaa caaggaagtt gagactctac gtgaagactt ccagaccgcc
    53941 agcctggcga atgcagcccc tgaactgctc tggactgtat ctgaccacct cggccccttt
    54001 accaccgctg accttattca gccggttgat aaactggtag acctgggcaa gtacgtgggc
    54061 agcgcaacag acgctgtcaa gacccccgat ggcaagacct ggggcgttcc cattgccaat
    54121 ggcaaccacc tgatgctgct cttcaataaa tccttgatgc cagttgcccc tgccaacacc
    54181 gatgaactcc tcaagatggg agcggatttt gttgcagcca accctgggaa atacgccctg
    54241 gccttcaatg agactgaagc attctggttt gtgccctggt tgggcggctt taagggcaaa
    54301 gtgttcgctg ctgatggcgt aactccaacc ctgaataccc ctgaaatgat tgagacgttt
    54361 aaattcctgg ctgaactcaa agccgccaat attatgcctg ccgagagcaa ctacgatgtg
    54421 atggacgcgg ccttcaaaga aggcacggcc gccatgatca tcaatggtga ctggtcgctg
    54481 ggtggataca aggaagccct gggtgaaaac ctgggtgttg ccccaatccc gaaggttgtt
    54541 gccactgata cctatcctgc tccttacacc tccggtgtgt tcttcatggt gccgaaaggc
    54601 ctcgagggcg agaaactgga cgcggttgtt gacttcatga aattcgcaac caacaaagag
    54661 aaccaggttg aaatggtgaa gaaattgacc cgcctgcctg cgctcactgc cgctctttca
    54721 gacaatgtga ttactgaaga ccccattctg gctggctctg ccgctcagat gagctacggt
    54781 acacccatgc ccaccaacgt tgaaatgcgc tgcgtttggg atgcgattaa acctgaactg
    54841 ctgcctacct atgcgggcac cacttcccca gaaaccgcag ccgccaacgc tcaaaaagcg
    54901 gctgaaacct gtatcgctgg cttagagtaa tccacaataa aagagaaggt gataaccttc
    54961 tcttttatca ataacacacg ccgggctgga tattcagccc ggcatatcct ataaatatag
    55021 tttggataag gaggaaatct catgatcaca tcagaggtga tcctgaaaag cctcggagaa
    55081 gtgggtacga cggtactcct gattcttatt ggaacagtcc tggtagaagc gttgctttac
    55141 ctgattctaa tgaaatggtt gaaatataaa tacgcgctac cttttatgtt attggctcca
    55201 gccgcaatcg gccttatcgc actggtgatt tatccgctga tttgggaact tcgactttct
    55261 ttcacaaata tgtcattaaa ggcttttcgc gacccggcat ttattggcat caaaaactat
    55321 gttcgcgttt tcaccgaacc agtattaaaa caaaaaatgt ttttccctct attgggacag
    55381 acaatcttgt ggaccgtcat taatatcacc ttccacgtgg ggggcgggct gggtttggcg
    55441 atgctcctca atcggaagat gagaggcaaa ggtatctatc gggcgatcat tatcttgcct
    55501 tgggctgtgc cgcaggtcat cgcggtgctg gcctggcgag gagagttcaa cttcgcctat
    55561 ggatacttca atatcatctt aacgaaactg ggtcttccag gagttcaatg gttggcagac
    55621 ccggtggcta actttgctgc catgtgcatg acgaatatct ggctgggaat tccctttatg
    55681 atggtcaccc tgctgggtgg tttgcaaagt atttcctctg aattttacga agcggctgaa
    55741 atggatggcg caaacgcctg gcagcaattc agaaatatca ctcttccact cattcaacct
    55801 gtattaacac cagcgatatt attaggaaca ttctggacat tcaacaactt taatgttcct
    55861 ttcttcatca atcagaacga gttggaaagt tctgatattc tggtaaccgg actcttcagg
    55921 gctgcattcc agtacaaccg ctacggtttt tctgccgcat tcgctttcgt aatcttcgcg
    55981 ctgctcttgt tgttcaccat tttctatatg cgttacacca ggtctttaaa aggagcgtat
    56041 gaatcatgag cgccaagcat acaactccca aaaagtcatt cttccgcagc gggcgtgagg
    56101 actcaccctt caagagaatt ctcatccatc ttgtgttgat catttcatgc gtcatcgcga
    56161 ttttccccat tttgagaatc ctggcagtat ctctgcgccc gggcaaccgc ctgatgtcca
    56221 ccgacctggc catcattccg cctgacgcta cgcttgaatc ttaccggatc atcctgtttg
    56281 aaaagccttt cttgagttgg gtttggaaca gcctggcgat taccatctca actgccttga
    56341 tcggtgtcat cctggcggct actgctgctt acgccttttc gcgttggaag ttccccgggc
    56401 gttcggtagg gctggtcttt ttactggcaa cccagatgat acccgcctcg atgctgatga
    56461 ttccaatcta tatccttgcg atcaagctgg gcatggtggg cacgtatcgc ggtttggtca
    56521 tcgcatactg tgtcacctca atcccattca gcgtctggat tcttaaaggg tattacgata
    56581 cgatcccggt tgatcttgaa gaagccgcca ggatcgatgg ttgctcacag ttacaggctt
    56641 tcttgcgcgt attgctacca ttatcaaccc cggcgctggc cattaccttc ctgttcaatt
    56701 ttatggccgc ttggaacgaa tacctgctgg cacgcgttat gctgggatct caagagagtt
    56761 tgctaacctg gccgctggga ttgcaacgct tgcagggtca atttcaaacc cagtgggggc
    56821 agttctccgc gggctctatc ctggtgatgg tcccggtgat ggtgctgttc ctgtattcgt
    56881 caaaatacct tatttccggc ctgaccctgg gcggtgttaa aggttaatag aaccaaatga
    56941 gcgcataaaa ccccggattc tgctgggaac tccgggtatt ttggattaat tcatgtaaga
    57001 ggtgtgtcga tgaaaaagac cttcaaatat ctgatcttta tccttgtgct gggtttaatc
    57061 ttgagctcct gcacctctcc cactggagat acccgtaaag gcattggcag gaaggatgtg
    57121 ctctatctca atcttacctg gcatcaacat caacccttat attacaaaga tgaccagggt
    57181 gtttacacgc gaccatgggt gagggtgcac gcgaccaaag attattacga tatggctgcc
    57241 atgatcaagc cctacccgaa tgttcgggta accattaatt taaccccggt tctgatgaaa
    57301 cagctggatg attttacaca gaacggcgct aaggattatt attgggtgct ggcggaaaaa
    57361 cccgcagcgg agttgaccga cgcagacaaa acctttttac tttcccggtt ctttgatgca
    57421 aattgggata atatcattta ccgattcccg cgttacaagg agttactcaa caaacgtggc
    57481 ggtacaagcg cagaagccat cgctagcgca gtatcacaat ttagcgagca ggattttcgc
    57541 gacctgcaag tatggtttaa cctggcgtgg attgaccctg aattcctggc acagagccct
    57601 ttgaaagaac tggtggaaaa agaaagggga ttcagtgaag cggataaagc agttcttttc
    57661 aatgaggtgc ggaaaatcat ggagatggtc atcccggtgc acaaggaact gcaggacagc
    57721 gggcagatcc aggtgatcac caccccttac gcgcacccga tattgccgtt gatctacaat
    57781 tcaaacatcg cctctgttgg caacccagcg gctgaactcc ctgcaagatt ctcgtggccg
    57841 aacgatgcca ttgttcaact gcaaaaaagt gttgatatct atcagcaaca tttcggtcgg
    57901 gctcctattg gcttgtggcc gggagaggga gcggtatcag aagatatcat cccgctggtg
    57961 gcggacgcag ggtatcaatg gatgcaaaca ggtgaggttg ttctggcgca atcactgggg
    58021 ttgggtcaat ttacccgtga ttctgctgaa actgtgcagg aagccgatga attgtaccgc
    58081 ccttattatg tgaccacaaa agatggacga cgagtggcca ttttcttccg cgacctggtc
    58141 ctttctgata agctggggtt tacctactcg caaacgcccg gcgaacaggc ggcggctgac
    58201 ctgatgcagc ggctggaaaa tattcggcta caactgaaaa aacagaaggc gcaaggacct
    58261 catattgttt cgattgtctt agatggcgag aacgcctggg aatattatcc taacgatggt
    58321 aaggatttcc tgaacgccat gtaccgaatg ttgaatgaga gcgaaaccat taagatggtg
    58381 acgccaactg aataccttga actgtttcca aaacaacgag aactggacac tcttttccct
    58441 ggtgcctggt tcagccccaa ttatgatacc tggattggcg aagcagaaga aacccaagcc
    58501 tggaattact tgggcaaaac gcgtgaattt ttggccaaat atgacgttgg caaaacgaag
    58561 caagcctccc ctgaagccat tgccactgca caagactaca tgtacctcgc tgaaggttca
    58621 gactggttct ggtggtatgg cgctgaccag gactcaggag tggatgaata ctttgataca
    58681 ggattccgtg ccttactcgc aaaagtgtac gagtctttag gagaacccgt cccagccttt
    58741 gtcagggttc caatcatccc cagaagaccc gagcagcctg ttcaggcatt ctcgggaatg
    58801 atggcggtgg tggtagatgg gaaaaccgac cagaaagaat gggcaactgc ggcggcttat
    58861 cctgcgaatg gagaagatgg gctggaaatg ctgtattaca ccatggatgc tcagaatatt
    58921 tatttccgcc ttgattttga caaagaaaga gaagaaccgg aaagcgtagg gatttacatg
    58981 agcatcccga gcgccgcagc aaacgatcct ttcgcgttga cagaagacgc tccggacagc
    59041 ctgttattag ggatccccgc aagccatctt tttgaatgca acgcagggct tgtgagcggt
    59101 tacgaggtat ttgctggtaa atggcaattg aacccagaac tgaaggcaac cgctgagatg
    59161 ggggagggac tgcttgaact ggctatacca ttaacctcgc tgggcgaatt gtcggcgggt
    59221 gatgatatac gcgttgtcat tgttgtccag cccaatagcc aggttttacc cctttctgga
    59281 ccggctcaga tcgtgctgcc tgacctggga ttatctgacc tggtgcttga aataaatgac
    59341 cccgaagggg acgaccatgg accgggaacc tatacttacc cgaacgatgg cgttttctcg
    59401 aaacaaaact ttgatattga aagtttctcc gtcgccacag acgcgaaaaa tgtgatcttt
    59461 acgttcaagt tctatggccc tgtgcccaat ccatgggggt cggggatcaa cttatcgcta
    59521 caaacaatgg atgtgtatat tgaccaggac cccgggaaag gcacgggtaa ccgtatgctt
    59581 ctcccagggc gcaacgcttc cttgaccgcc aggaatggct gggagtacgt catttgggcg
    59641 gaaggctgga attatggaat ttacgcgcct gacccagcca ctggtgaacc aaaacaacaa
    59701 aactcatcgt acaagatcct cgttgaccct gccaggcaga cagttacctt acgtgtgccg
    59761 aaagaagctt taggcgatgg caacccggct gaatggggat acgccgcagt agtgctcagc
    59821 caggatggtt acccctccac tggtgtttgg cgagtaagag atgtgcagca gaccgctgct
    59881 cagtggaagc ttggcggcgc cccggacgat accaatcaca cccgcattat tgacctggca
    59941 tggccagcgg agggcaaggg cacgcaggaa gagtttttaa gcacctatat cagttcaaag
    60001 gcggctatcg aaacgctttc tgagaaggat atgccgcaaa tacccatcct gctggtgaaa
    60061 tagcatggta gaacctgctg ggatgaacaa cccagcaggt tcttgcttca ctggtaggga
    60121 tgttccattg ctaacaattc aattctgtga gaaaatgacg cagttttggc gaagacgatt
    60181 cacctgttct attgaggatg gtatacatga cgattcagac tcctgattgg cttaaagacg
    60241 cggtttttta ccagattttt ccggaccgct ttgcaaaaag tgaacgggtc aaacgcgaag
    60301 ggctgaaact ggagccgtgg gacactccgc ccaacccata tggttttaaa ggcggcgatc
    60361 tttacggtgt cattgaaaaa ctggattacc tggtcgacct gggggttaac gccatctact
    60421 ttaaccccat taacgcatcc gcctccaacc atcgttacca tacatatgat tatttcaaga
    60481 tcgacccgct tttgggtggt gatgaagcct tcagggatct gctgcatgcc gcgcacagcc
    60541 gcggaatgca catcgtacca gatggtgtat ttaatcatgc cagccggggt ttctggcagt
    60601 ttcatcatgt gcttgaaaat ggagaaggat cgccttatgt cgattggttc tatttcgacc
    60661 cggagcgatt aaaacggcac aggccttggg gggcttaccc tgacgaaaga gaacaacaac
    60721 aattgaacaa cggagtcggt tccttcgatg ccatcgggta tacggcttgg tggaacctgc
    60781 cagcgctacc gaaattcaac acgcaaactc aagcggtccg agagttcatc tttgaagtcg
    60841 cggagtactg gctgcgtttt ggcgcagatg gctggcggct ggatgtgccg ggggagatca
    60901 atgatgactc cttctggcag gagttccgcc gcagggtgaa agccgtaaac cctgatgcct
    60961 atatcgttgg agaattatgg cttgaatcgc aacgttggtt aaaaggcgac caattcgacg
    61021 cggtaatgaa ttacctggtc agcgccgcac tgatgggttg gctgattggt gataagatgc
    61081 cagaatttgc ctaccgcatc ggtggattac aaagagtgat gcgaccgttt agcgcagccg
    61141 aatttggcga ccgcatcgaa tacctgttga acctgtatga cccggagatc aattacgcgc
    61201 aactcaatct gctggacagc catgacactc cgcgatttat cactacagcc agcggcgatg
    61261 aaagtgcgtt aaggctggct tacctgttcc tgttcacttt catcggcgcg ccttgtattt
    61321 attatggcga cgaggtgggg ttggacggcg gccctgaccc tgaatgccgg aagtcttttc
    61381 cctgggatga gtcacgctgg aatcaagggt tacacgctta tatgaaagac ctgatccatt
    61441 tgcgtaacgc gcacccagca ttgcgccgcg gcagttataa gagaattttt tcagacaacg
    61501 aagcgcttgc tttcatccgc cagtatatgg gcgagacgta cctggtggtc atcaacgcgg
    61561 gttcaaaaga acgccaactc cacctcgatc taactcaaac aggcgtgtct tcgcgcgcgg
    61621 tcgaatcgct gtttggactt ggacgggcga cgctggaagg cggctctctg tgtattgagc
    61681 acctgccttc aaggcaagga gttgtatata ggttggagta gacgcctgaa gatgagtacg
    61741 ataaaccgcc ggtgaattac tgaatatccg gcggttttat tttatagagc cctttcattt
    61801 tatgacctgc cgcaacggtt agtgcgaatt gtgtggcatg gtaaaataca tttccgattc
    61861 atgcttgatg aaacctgcat attaaaaaat aaaagagtta gggtattgcc gcatgtcaga
    61921 ttccaacctg aatggtcaat tgaaagtaaa ttcacccagg ccaaccattg agatcgtttt
    61981 gcccgacgga cgggtttacg ctggtaatcg gggtgcttca ctgtgtgaac tgcttcagat
    62041 gctcccggaa tgggataacc ctcccatcat gggcgcagtg gtcaacggag agctgaaaga
    62101 gttgaccgcc acgctcgaaa tggacgcgcg cgtgcgcctg gttacgatga aggatgacga
    62161 tggcgccaga atctaccgac gttcgatcac tttcttgctc gaagccgctt tcgaagagct
    62221 gtttccgaaa agcttgatgg cgcttgacca ttcggtctct gctggcgggt attactgcca
    62281 ggtcagcaag ggaaaccagt tgggcagcga ggacctttcc cgacttgagc tgcgcatgcg
    62341 tgagcttgtg gaagcagacc tgccttttga acgccaactg gtgccgttgg acgaagccat
    62401 ctcttacttt gaatccaaag gcctaaaaga caaagttcaa ctgctgaaat atcgacaaaa
    62461 ggaatacctg gttctgtatc ggctgagtga acatcgtgat tatcaccacg gatacatggt
    62521 gccttccacc ggctacctga agtggttcgc attgcagcaa atgggagagg gattcgtgct
    62581 gcgcttcccg agaagggaat ctcccaagca actttcgccc atgcccagct accccaaatt
    62641 gctgagcacg ttcaggcagt atggtaactg gcttcagcga ctgggaatcg ggtcagttgg
    62701 cgagctgaac gcctctattg acgcaggcaa tattcgagaa attattttag tgtctgaagc
    62761 gctgcataac ctgcaaatca tcgatatttc tcaatctatc gcggataaat ctgaggaagc
    62821 ccgtatcatc ctcatcgccg ggccttcttc ttctggcaaa acgacttttt ccaagcgttt
    62881 gtccatccag cttttggcgc agggtttctc tccctttccg atagagatgg acaactattt
    62941 tgttgatcgt gaaaaaacgc cgaaggatga attcggtcag tataattttg aagctttagg
    63001 ggctctggat actcatttgc tttctgatca tctaaagcgt ttgattcaag gcgaaaaagt
    63061 gaggttgccc aagtatgatt ttcacaccgg caagcgccag caaggcgaag aaattcaact
    63121 gcgccatgac cagatgatca tactagaggg cattcatggc ttgaacccgc aattgctgcc
    63181 ggagattgac ccatcctcga cctacaagat ttacgtttcc tgcctgaccc agctcaacct
    63241 ggaccgctac aaccggattt ctaccaccga tacccggctg ttgcggcgta ttgtgaggga
    63301 cgcgcgtgaa cgaggataca ccgcccagga gacgatttct cgctgggaat ctgtgcaaca
    63361 cggagaaaag gaatacattt ttccgtacca ggagaatgcc gataaactgt tcaattccgc
    63421 gctggtgtat gagctttctg ctttgcgcct gcacgccgaa ccattattgc gccaggttcc
    63481 atacgggact aaagaatatg ttgaggcgaa gcgcctgttg gcattcctgg aatggttcct
    63541 acccattgac tcccgcatga ttcctgacaa ttcattgatg ttggagtttc tgggcggttc
    63601 gattcttaaa gattttatac tgtggaaata gcagaaaaga acgggttcag aaccgctgag
    63661 cccgttcttt ttattaagcg atttttttct taaaagccct gcccaggcgg gcaattcctt
    63721 cgttaatccg ttctggtgag caaaaagaga aatttaaacg catggtgttt tttccgctcc
    63781 catccgggtg gaagaactct ccggggacat aagccacctt ctcctgaatc acttcaggca
    63841 gcatttctgt cgtgtcgaat tgttcaggca gggttaccca taagaaaagc cctccatgag
    63901 gtcgtgtcca cttcactcct tcgggcatgt gttcttccaa ggaatcgagc atggcgttgc
    63961 ggcgttcccg gtagactttt tggatgaccg tgatgtggcg gtcaatgaag ccatgctgcc
    64021 cgacctcgtg tgcaacaatc tgattgaaag tggaggtgtg tagatcgcaa ccctgcttgg
    64081 ccagcgccaa cttgctgatc actccatgtg gggcgatgac ccaggctaaa cgcaaccccg
    64141 gcgccaggat tttggagaag gtgctcaggt aaatgacgtt gcccgtatag gtgccatttt
    64201 gaacgcgcat ttgcgaatcg agcagttcta ccgcgggcag gctttcgcct tcatagcgta
    64261 actgaccata ggggtcatct tctacaatcg gtacgccata gcggtctgct aggtgtatca
    64321 gctttttccg cctttccaag gagagagttg tgccggtggg gttttggaag ttcggcagca
    64381 cataaataaa tttcgggccg atgcgcagag ctgcttccag tttctcggtg atcatgccat
    64441 attcatcaac tgggacggta atgtattctg ctccgtatgc gttccacgcc tgcagtgcac
    64501 ccaggtaggt gggtgattcc accaggatgc ggtctccgcg gttgatgaat attttcccca
    64561 acaggtcgag cgcctgttga gacccgctgg tgatgaggat gttgccaatg tcaacattaa
    64621 ttcccaggtt gatggcatgc ctcaggatca tctcccgcaa gggaacatag ccatctgtcc
    64681 caccgtactg gagtacttca gctcccttgt gttcaaggac gtagttacac gctttcttga
    64741 actcttcaac cggaaataca tcaggcgctg gaaaacctcc gccaaacgag atcatgtctg
    64801 gttgttctgt taccttgaga agttcccgta tggccgatgc tttcatacgc tgtgtgcgct
    64861 gcgagtagcg gtattcccac ggtgtctgca tttttgtgtg catttttcct cttggttttt
    64921 agaatgattt ttgccttaca acctggcaaa acgactttca acagttgccc gggagggcag
    64981 ggttacccgt tctcctggat tcaattggga aggcgccttg cctgcctgag acggtgctcc
    65041 ctggtaaatt aaaatgacgg tgccttcaac tgcgtacctg gtttcaataa aagcctgacc
    65101 agtgagcaaa ccgtctttat cgatcgcgca actggtcacg tgaccaatga ccctgccttt
    65161 ttcatccagt accgggtcgt tattgtgcgc catgcgcacg cctttttcgg caaagcggaa
    65221 acgaacgaca actccttttc gggtaagttc cttcttaatg aaggattccc ttcctataaa
    65281 ccagggtttg taggttttta cataggaacc aaacccggct tcagcaacgc ccaggttgcg
    65341 ctcaccaccc atttcatgcc catacagggg gaggccggct tccgtgcgca aagaatctcg
    65401 ggcgccgaga ccgcagggct taagccccaa aggttcacca actttcatca ggtcgttcca
    65461 taggcttgag acgttttcag ggtgtacgaa aagctcaaaa gccattttct caccggtgta
    65521 accggtacgc gagataatca gatcgtaccc gccaatcacc gcttcgcaaa gttcagtgcg
    65581 tttgagggct tttacttttc tctgcgtggc ggcatcacaa cccaacgcca gcaagatgtc
    65641 gcgtgatttg ggcccttgca acgcgatatc cacgcgcata tcggcacctt cgcgagggtc
    65701 gcgcaggttg cgtaaaacca ccccgcgacc aaaagctttg acccaggggc ggtgattgtc
    65761 gaccgctaca ctggcattgc ggacggcgtt cagccaggcc cagtctttcg catcattcga
    65821 ggcgttgacc acaacaaggt atttatccgc gctgcggcgg tagacgaggg tgtcgtcaat
    65881 cacattggca tccgggtcaa gaaaatgcgt gtagcaagaa tccccaatct ccaacgaacc
    65941 aatatcattt ccgcaaactg aatccaggaa gagagccgct tgcgaccctt ctgcctggaa
    66001 aacgcccatg tgagcgacat cgaaaagtcc ggcggcgtta cggcaggcca ggtgttcttc
    66061 aactaccgag gtataccaga ccggcatttc ccatccggcg aaaggaatga gtttggcacc
    66121 cagtctctta tggtcttcaa aaagaggggt acgcatgagc tcgttggaag acttttcctc
    66181 ccatttaaac gatggaagag cgttgccgtt tgaatttgtt ataccgatgt accaggcttt
    66241 gttgtttgcc atttctttac ccgagctggg cattggcgcg accaattctg attcttccac
    66301 cacaactggg ccggggatac gcgcaggcag gtcgttatca aaagcgatgt aaccatcaga
    66361 cagggcgcgc aagaaggttg cagccagacc aaaattttct gcctggaagg aaaacctgta
    66421 gaggttatct tccactctgg taagtgaacc ttccactttt ttcagcgggg tgatgagcga
    66481 ggttggttgg gtctgaccgt tttgcaaggc ttccacatcg gagctaaagc aatagttcac
    66541 aaagtacaga acccggtcac ctttaaggtc caatgaaacc agtttcccct tgaaccgggg
    66601 ttggtcatcc aggtagtaaa aatgcgggta gccgctgttg ccatgcggga gatcagttgc
    66661 agcgctggcg caaaggttgc ggaccttgat tttcgtttgc tctagaacat taaaatcaat
    66721 tttggcgcgc ggtgaaggac cttttcgcat ttcaatggtg tacggcgtaa tcgcctgcaa
    66781 gagatcggca ataaggttgc caatttcgcg catattttcc gcggtcaaac cgcgttgagt
    66841 catccagggc gtgccatagc ggatgcccga agcattgagg gcggttttat cccctggaat
    66901 ggtgttgcgg ttgagcacaa ttcccgcgat atccaggatg cgggcagcca tgtcaccgct
    66961 cagagtggtg ccatcaggac cgacgatagt tttgcaatcg atgttgccca ggtgagagtc
    67021 agtcccgttg aacgggatgc gcagcccgcg agccttcaat tgagcggtga gggcgacgca
    67081 attttggatg atttgttttt gtaactgctg aaactcagcc gttgcggcga ttttaaacgt
    67141 tgtggcgaga gcagcgaaca catgcacatg aggtccgccc tgctcgccgg ggaaaaccgc
    67201 tttgtcgatc ttctttgcca gcgcgctgtc tgtagtgagg atgcatgcgc cgcgcggtcc
    67261 gcacagggtt ttatgcgtgg taaaggtgat cacgtgagcg tgaccgaccg gagagggaat
    67321 ggctttcccg gcgatcaagc cagcgatgtg cgacacatcc gcaagcaggt atgcgccgac
    67381 ggcgtctgcg atggcgcgga agcgcgacca atcaggaatc cagggataag aggaataacc
    67441 gcagatgatg atcctgggca tgacttcttt cgcctgcgct tccagctttt cgtagtctat
    67501 ctgttcggtt tccgggtcaa cattgtaatg agcgaccttg taaagttttc cggaacggtt
    67561 cacagatgaa ccatgtgtta gatgaccgcc atgcagcaag ttcatgccta agatggtgtc
    67621 accaggttcg atcagcgcat ggtacaccgc gttgttggct ggagcgcctg agagtgcctg
    67681 gacattgaca taaagttgat ctgcggtgac cctgtcagtg gcgaaaacct ccgcacagcg
    67741 ccgccgcgcc agcgcttcga ccacatcagc atattcgacc cctttatagt aacgaggatc
    67801 agcgtatcgt cggtaatagg ataaacggac tggatagtcc aggatttctt tttcactcat
    67861 caaacgggtt tcttcgtcgg gataaccttc cgcgtatagg ttttggaaaa cggaggagag
    67921 agaatcacgg acggcttgcg gcgcggtgct ttcagagggg atgagaatca gtttacggaa
    67981 ctgtctttca acttcgtaat taacgaggtt aaaaactgcg ggatcgatct ccgacagaga
    68041 tcctcgtacc agatagtcag tcatgtaata aactccttct tgatgtgagt gggaaaaaat
    68101 aaaaaaccac cttcagatgt tggccataca agcatctggg ggtggttgga atggagtaat
    68161 tatgagtcat tcgtttctca gtaaccgatc taaaagcacg atcttgattt caggttctgt
    68221 atcgcaacga tagtgacaag gttattttag ccttaacccc tctatgggtc aagcaaaatc
    68281 tggaagtgat aaaaagggga ggcattgcct accgttcaaa gcagatacac acgcaagacg
    68341 atcgtgtgcc ctgatgttta tcacgctttt cgcccttata ataaagaatc tgccggaaca
    68401 aatgatggtt gagaaaagcc gatcaatgga agtgaggacg tgattacacc cgaaaacaca
    68461 atcaatgaac atccggaatt ggattacagc ctgaaaaagg ccaggaagat gggcgttatt
    68521 ttgttgatat tttttactac gctctgggta ttgtctctga ccgggttaaa ccttttcctg
    68581 cgctggtcta ttgaacaaag catgtttgaa acagaaacag gcatacagga tgttcgctgg
    68641 ttggttcatt tagtgtgtgg tgttttcttg tttatcccgt tgctggcttt gtccttcgca
    68701 gtgaaagttc cacgcgtcaa aatcatgttg cgtctctgga cggtgggcgc ggcttttgtg
    68761 ctgctttcag tcccgcccaa ggtgctctat ctcaccgcgc aggttcagtc caatctgctt
    68821 ctttgcggag tcctattact gatgaccctg gtgttgtttt tattaaggaa taagcaaacg
    68881 agtggatcga agacgcttcc tccatcaggg ttggccggac cggttttttt agcctgcgcg
    68941 gccctggcaa caccctgggt gatgtggggc gcgttgggtt ccgtggagga ctcgctttcc
    69001 gcgttgctgc tcggcgcagc atttgcgctt tttttcatca acgcggtcgt agtggattac
    69061 ttcgacaaga ctcagccccc tggcagcctg gtgaacagga aggatttcct gtttgacggc
    69121 tttgtgttgt tcattttttt gtttgtcgtt gtcagcgggc taagccagaa cggatctcaa
    69181 attgtgctct gtctttccat ccccgcttgt gggtggttgt tggcagcgct atctaccgcg
    69241 gggcgcaacc ggaaaaatca tgcccggctt gccacgggcc ttgtttcagg cctgatgctg
    69301 gcgctgccgt tggcgttttt tgatatggat gaactggcat tgttcatctc tggaactgat
    69361 ggagaagtgt ttttctgggc aaacagggcg gggtggaaca ctctgacctt ccaaatcgcg
    69421 tttgcgctgg tggtgttgat atttttcaaa caacttgagc gcgttcacct gggcagacgt
    69481 atcaacgcga tgctgctggt attaagcctg gcggaggtcc tcgttctata tttcacggcg
    69541 ggacaacccg gtttttttgg tgaccgcctt ttcgttgtca tgaaagagca ggtgaaccct
    69601 acccaattca atgaaataaa ccctcttctg gagaaaaaac tcgccctata ccaggccctg
    69661 accgaaaccg ctgtaaccac gcaagaagaa gtgaccaccc aacttgcaaa atggcatatc
    69721 gactttacgc gttattacct ggtcaatggg ttagaggtaa acacaggcgt tttgatgaag
    69781 agcatgctgg aaaaaatgga cggcgttgac cgggtgctgg acagcccgcg cctccgcccg
    69841 ctccctcaac ctccgccgcg gatggagggt gaaataacta ttcctcctga agattctttg
    69901 tggaacctgc aaatgatcaa tctgcccctg gtgcatgaac aactgggtgt ttttggagag
    69961 ggcatcatca ttgggcaaac ggattctgga gtggatggct cacatccgga attggcgggt
    70021 tcttacaggg gagccaaagg cagcaacgat accaactggt tggatccctg gaatcatact
    70081 gttgcaccca atgacctgag tggacatgga actggcacca ccgcgatcat tgtcggcgcg
    70141 acgcgcggta ttgcccccaa ggctcaatgg atagcctgca cgaacctggc caggaacctg
    70201 ggaaacccgg ctctctacct ggattgtatg cagttcatgt ttgctcccta tccacagggg
    70261 ggagacccgc ttctgcatgg aaagcctgaa ctgggagcca tggtcgtgaa taactcatgg
    70321 ggctgcccca ggattgaagg ctgcgatgcg gatgttttta agccagcggt tgaaatattg
    70381 gaagccgcag ggatttttat gtctgtttcc gccgggaata atgggtactt tgggtgttcg
    70441 acaatctccg acccgcccgc tctttacgag catgttttct ccgtcggctc agttgacagc
    70501 gcaggagagc tctctgcctt ttcgagcaag ggtcctgtta ccagtgatgg tagcgggcgc
    70561 agcaaacctg acttgctggc gcccggagag caggtattgg ttgccgcacc agggggaacc
    70621 tatacttaca gcagtggcac ttcttttgct gcgccacatg tgactggtgt ggtcgctttg
    70681 atgtggtctg ccaatccaaa actcatcgga gaggttgccc tcaccaggca aatccttcaa
    70741 gaaagtgcac aaccctacac agacgggtcc actagttgtg ggaccccaca aaatgaaagc
    70801 ggcgccggca ttttggacgc gtacgctgcg gtcaaagcag cgattgcggt aaaatgaatg
    70861 aaaggattga accttttctt tggaaaaagg gttaaatagt tgaagaaaca aagcgctgca
    70921 ccaacagagt cattgaccga tcagaacgct gttcaacttg cctgtaacgg caacgcagag
    70981 gcgtttagct acctctatga acagaacgtg aagcgcattt acaattacat tttttatcga
    71041 atcggcgctg aagcggacgc agaggacctg accgcgagag tttttcaccg cgcattcgcg
    71101 cacattaaca gttatgttga aaaaggagtg cctttcactg cctggctgta tcgcatcgct
    71161 cataacctca cggccaactg gtaccgcgac aatcatcgaa ggcgagaagt atcattagat
    71221 gaccattttg atttacctca cctcagcgat ttacctgaac gcactattga aatgaaccag
    71281 gaaaaggaac tgctcctgaa agccattcgg cgattacctg ctgaccgcca acaacttatt
    71341 ttgctcaagt ttcttgaaga tctttcaaac ggtcagatcg ccattattat ggggagaacc
    71401 gaaggcgcaa taaaaagcct ttatcaccgc tcgctgatct cgttgaggga agaattaaaa
    71461 cggatcagcg gcgttgagac atcggatttt cgataagggg tatggaatat ggaacatggc
    71521 ctagaacaaa atggaagacc tggtttatgg gggttggagc aaaagctctc caacctgctt
    71581 aaaccagtga cgcctgaccc tgaatttgtc gactcactaa aaatgaagtt gacccgcgct
    71641 ccggccgtgt tagttgaaac cagccggcgt aaaatggggc ttctggtggt gagttttgga
    71701 ttgttcgccg gtgctttttt ggtttggctt ttgcgcaaac gatggcagtg agcgctgctt
    71761 ataaccctaa tttttctttt aattgataag tcgagggctc gacgagtatt gcgccatcag
    71821 ggaagacgat tgttggcaca ctgcgaaatc cgttcgctac gctttcaacg aacttagccg
    71881 cggtctcatc ctggtcaata tcgatccact catagtcgat cttttcatct tcaaacaacc
    71941 ttctggcgcg cttggttgaa ccacaccagg ttgtgccgta catcataatc ttcttctgcg
    72001 tatcacccac ttactctgat acctccttca ataaagtttg gatcaccgcc tgcgcgtcag
    72061 cgagcaggtc aatcccggtt ggtagggcgt aattttcatc gacccgcatg gtcaggtgtt
    72121 gaagggcagg aaggacgctc gcgggcaggc tttttcgcac agactcatca acgaggatct
    72181 cgaaattgtt caagcctgca gcttcaattc catgttgatc aaggaaatcc ctgacgacga
    72241 tgccagcggc ccgccgcgcg cataccctgg ccattccttc gttgccttgt tttcttgcag
    72301 cgaaggccat gtccatttct ttctgaattc tctcacttat tttcatgggg caattctaaa
    72361 ccatttcgca tcttttgcgg cagggttctg ttttcgtttc gtggcttatg ttaaaatcaa
    72421 acgcatgagc gagaaaaccg tcttcatggg gtcacccgaa ttcgccgcga gcatccttgc
    72481 tgcactggga aaccactatg acgttcgtgg tgtggtcact caaccagaca agcctgcagg
    72541 gcgcggaaaa aaactcactc cccctccagt gaaaacgctg gcggagcgtt tgggttaccc
    72601 tgtcattcag ccggaaaaaa tgaaagaccc aggtgtgttt gaacaactgc atgcctggaa
    72661 tcctgatttc attgttgtgg cggcatttgg caagattctc cgtcaaaatg tgttgaacct
    72721 tccgcggtta ggttgcatca atgtgcacgc ttcctacctc ccgcgctggc gcggcgccgc
    72781 gcctgtccag gcggcgatca tccatggtga tactaccacc ggggtcagca tcatgaaaat
    72841 ggacatagga gtggataccg gtcctatcct gattcgcgag cgggtgaaaa ttgaacccgc
    72901 tgatgatgct gcttcactaa ccagccggct ggccacggtg ggggcgaccc tgctgctaca
    72961 ggcactggat ggatacatta atggcaacct gtcgcctgta cctcaaaatg agaacggcgc
    73021 cacttacgcg ccgatgatca ataaagagga tgggttactg gatttctcac aggcagccga
    73081 gatgctggaa cgtaagatta gagcgtatag cgaatggccg ggcgcctaca tacggttggg
    73141 tgaagaagca ctgaaggtcc gccgggcgaa agttgtgcct ggcagcgggg tcccagggca
    73201 gcgcgaaacc tgtaatgggt atccttgcat tcacacaagc gatggcaaac ttgtgctgct
    73261 ggaagtgaaa cccgctggaa agaactggat gaatgccgca gactacctgc gcggttcccg
    73321 ctcctggatt tcaaactgat tgattattta gaacaaaccg ccgcagagta ttgtgctcaa
    73381 tgggacagcg cgctgatggt tagcggggtg ataggaagac tgatggttct taaacatcac
    73441 cgttaggaat gtattgggtt agaacctcga aatggcgatt gtgggtatta agattcctca
    73501 gcggataaaa aacgcctgcg ccactcgtag aggatgttca gggcttctat cggtgggagg
    73561 gtgttgaggt cgatggcttt taactcatcc aacaaagggt ttgtttcggg gaaaagggtt
    73621 aactgctgtg aggaggggag atctacttta atggtggaac ctgaagaagc ttcgagttgc
    73681 tgtaggatct cactggcgcg cgaaattacc gggcgcggaa tgcctgccag ttgcgccacg
    73741 tggataccgt atgaacgatc agtgccgccg gggacgatct tgtgtagaaa gatcacctgg
    73801 gtgccatttt cagctaccgc cacgttgtag tttcggatgc ctggcaacag atcactcaac
    73861 tgtgttaatt cgtggtaatg agtggcaaac agagtacggg ctcgcaatct ggggtgattg
    73921 tgtatgtatt caatcaccga ccaggcgatt gacagcccat cgtatgtgga tgtaccgcgt
    73981 cctatctcat ccagaatgag caggctgcgg ctgctggcgt ggttgagaat gttggcggtc
    74041 tcgatcatct caaccataaa agtggattga ccggtgtgga tctcatcctg ggcaccaatg
    74101 cgagtaaaaa tccggtcaac gatgccaatg gtggcgctgg cagcaggcac gtaagaccca
    74161 atttgcgcca ttaacacaat caacgctacc tgccgcaaga aggttgactt gccgctcata
    74221 ttggggccgg tgatgatgcg gataatctcg cctgtttcca tcacggcatc attgggcacg
    74281 tatcgctcac tttgcagcgt ggtttccacg acggggtgtc taccatcgac aatgttgagc
    74341 atgggttctt ctgctagaac cggacgaacg tatgcatgta tggcggttac ttcggccagg
    74401 ctgctcaaca catccagctc acccagcagg cgagcgcttt tcagcaaggc agataaatgc
    74461 ggcagaagcc gctggcaaac ctctttgaaa agccgatttt caatttccct gatgcgctct
    74521 tccgcgttca ataccagcgc ttcatactct ttcatctcag gcgtgatgta tcgttctgcg
    74581 ttaaccagag tttgttttcg aatgtattct gctggcacct gcgcggtgtt gctgtgggtg
    74641 atttcaagat aatagccaaa caccttgttg tagccgactt tgagggattt gatgccggtc
    74701 ctctcacgct ctacggcttc caggttggag atccactctc gcgcgtggtt ggacatttcc
    74761 accacctgat ccagttcttc tgagaaacca accctgatca cgccggtatg ctgcagtgtg
    74821 gctggcggtt catctgcgag cgattgttcc agtaagttga gaacttcagg gcaaaggtca
    74881 aagtcgatta acttgtgaga gagctgcaag acgtcttgcg aaagggtatc gcggatggct
    74941 ggcagtttct gcaggatggc acggatggca gccagatctc ttgggagagc cacgtttccg
    75001 gtaaggcgat ttgccaaacg ctccaaatcg ccaaggtgct gcaactgcac acgaaaatca
    75061 gcgcgaacca atgaatgctg gtgaaaaaca gcaacaatgt ccaatcgctc attaatgcta
    75121 tcaatcctta acaatggctt actgacccat tgccgcatgc aacgatgccc catgggagtt
    75181 aaggtgtggt caaggattga aagcagcgac ccctgagttt cgccgcggcg gatggtttcg
    75241 gtcaattcca ggtttctacg ggtggccgcg tccaatgtca taaattcgcc cgtgctgtaa
    75301 gtggagatgc ttttcaccat ggataacgcg gctggttggg tttgttccag gtactgcaaa
    75361 atggcgccgc tggtttgaat ggcggaagga gcattctcca ggccgtaccc ttctaacgtg
    75421 gtcacttgca ttacccgcag caatacctcc cggcagcgcg aagtttcaaa gcgccagtct
    75481 ggaaggctgg taatatgacc agaaaacgct gtgttcagtt tgagcgattc gggcaccaat
    75541 atttctgccg ggtccaacct cgtgagttct gcccgtaaat cggataatgg atcacgcgat
    75601 tcgattgtgg tgacagcgaa ctcaccagtg gtgatatccg cgtaagcaac gctcaactgg
    75661 ccatcttcta cacgcgccgc tgccaggtaa ttattccggt tgctgtgcaa caatcctggc
    75721 tcaatcactg ttccaggcgt taccacgcgc acgacttcgc gcgggaaaat tcccttggcg
    75781 ggttgttccc ccacctgctc acaaatggcg acatgatagc ctttatcgat taaccgggcg
    75841 aggtagtttt cagccgcatg gtaggggata ccagccatcg gaacgcgttg ccccttggcc
    75901 acattacgag aggtgagcac aatatcgagc tcccgcgaag tgatttgggc atcatgatca
    75961 aaagtttcgt aaaaatcccc caggcggaag aacacaatcg cttcaggata ttgctttttt
    76021 atatccaaat actgctgccg aataggggta acatcttctg ccatgaatcg tccaaatcaa
    76081 gtcgatacga taaaacttaa cagatagtaa atggaaaaga aaatggattg agggttacaa
    76141 cttctgcccg cattcgccgc agaacttggc atcgggaagg atttttgacc cgcaatgtgg
    76201 gcatttgtct ttcggtttca aggcgtaacc gcactccggg caaaatttcg cgttggctgc
    76261 taatggtttc tcacagttgg ggcaggtggc ggtgatccct tcgcgccaat attcagtgcc
    76321 cagtttttta tcctcctcag ccatggctgc atgagcccag acttcctcaa cggattttga
    76381 agcctgagca gccgccattt ccacgcccag gtctggagcg cagtctttgc acaagccttt
    76441 ctttttgttc caacattttt cacggcatac ccaggcgttg cagcgcgggc attgaatgaa
    76501 atatggtcga atttctttga ccgcttcagt gaagttttcg tcatgggctc gttcccaggc
    76561 cgctgaatga acgcgatcac tgatattcgc cgcctggctg aggaaccctc caaacaagct
    76621 gctggcagta tccaatgccc cgctcagttt acctgtcaag gaaggtttga aggttgtacg
    76681 gtagcctgat tcgcagcggg cgcaggtaaa ttcaaattga aaacccctgt cagtggacag
    76741 gtcagtgtag ttcttcacga attcaaggtg ttcaaccatg ataccctccc ataatcgaaa
    76801 atgagtaaac cttattaatt atatcccaaa tggtttgggc cttctaaagt ggttataatt
    76861 cattttatta caattgagga tcaagatgca aagagttcag acatgcgata tttttacaag
    76921 gattcaattg gggattggca cgtgggcctg gggagataaa ctggtttggg ggtatgggaa
    76981 tagttattcc gaagcagata tccgtgaggc tttcaaggta tgcgtcggtg gtgggatacg
    77041 gttttttgat tcagcggaag tttacggtca gggaaaagcc gaaaccatcc tgggcgagct
    77101 ttgcgcccag gaagatgaag aatttgttat cgcgacaaaa atgatgccat ttccctggcg
    77161 cctttttcat aattcattga aattcgcgct gcagaacagt ttgcgccggc tgcgggtgaa
    77221 ccaggtgcag ctttaccaga tgcattggcc gctaccgccg gtaaaggtgg atacctggat
    77281 ggatcggatg gcagaagtgg tggatgcggg gatggtcaaa gcagttggtg tttcgaacta
    77341 caacctggaa caaacgataa ccgcacacga agcgcttaag aagagaggat tgcggctggc
    77401 ctcgaaccag gtagagtacc atttgcttga gaggcgaatt gaacaaaatg ggttgatgga
    77461 gtattgcctg gaagagggga ttaagatcat tgcgtatagc ccgttggcta tgggtatctt
    77521 gaccggtaag tatacgccgg aaaatccacc ggctggcaca cgagcagccc agtacaatcg
    77581 gtcttacctg gaaaagatac agcctttgat aaaaacgttg acccgtatcg gcaatgacgc
    77641 tgacggcaag tcggccgggc aagtggcgat taactggtgc attcaaaaag gcacattacc
    77701 catcccgggg gtaaaaaatg ctgcccaggc agttcaaaat ttgggggcta tcggctggca
    77761 attggacgca gaggaaatgg cgcttttgga tgaggtcagc cttgcagtaa ccctacccta
    77821 agcaagggct tgtttactca acaaattcca gttcctggtc gtccaatgta gaaaattcac
    77881 cttctggctg cagagaaatc tcaccgaaga gctgattttg gtcggcgccg accatatgcc
    77941 tttttacgag ttccagtaaa gccaggaagg tgaccaccac ttctactcgg ctggagcggg
    78001 tggtgatgag ctgttggaag gttgtagcgc ccattttttg cagcgcttcg agaatgacat
    78061 gaatcttctg acgaatcgtg aagcgcggtg acgcgaccac ttcactcaaa ggcggtaaat
    78121 tattaggatt tgaaaagacc tctcgagccg cttccacaag ggcttcgagg gtcaattcac
    78181 tgatatccag tttagaggga atttgtaccg ctgggttcgc gtcgaggcgc aaataggtgt
    78241 gatagcccgc ttcttcgcga gattctaaaa acctggacag gtctttgaat tttttataaa
    78301 caaggagctg ccgtgccagt gcttcgccgg gatcttcttc gacctgcaag ctgggaatgg
    78361 tttgccttgg taaaagggca gcggatttaa tctgcaccag cttggcggca attacgagaa
    78421 acgcggagac ttcagccggg tcttgagttt cgaggtgatg catatactca aggtactgat
    78481 cggtcacctg cgccagagaa agacgggtga tgtccagttc cgccttttca atcaactgga
    78541 gtaggagatc cagagggcct gaataaacct cggtgttgat cttatatcct tctgtttgat
    78601 gtcccgcgat ttgaaagtcc acgccggtta ttatagcaga ttgcggtcag gcatattcta
    78661 tgctataaaa gcagggaacg agatacccat gatgaagagc gggacgatta attgcacgta
    78721 ttgcggtgag ccttatccat ccgcagggct acctttccgc tgtacttgtg ggggtatcta
    78781 cgatttttct cgtttccctg atttcaccat tcccaaagct caggcggtac ctcgcggagt
    78841 atggcatttc aaggattctt tagggctgcc cgctgaggcg cccatcgtat cgcttggcga
    78901 agggaacaca cctttgatgt cgtggtctct tcatcatcga caggtttatc tcaaaatgga
    78961 atgccagaac cccactggtt cttacaaaga tcggggcaca gccgtgctga ccagtttttt
    79021 gctggcgcgt ggggtaaagg aggtggtgga agattcctct ggcaacgctg gcgcttctct
    79081 ggcagcctat tgcgccaggg cggggattaa agcggcgatc tttatccccg agtcagcgtc
    79141 tgggcccaaa cgctggcaga ttgagagata tggagcagag gttcaccgtg ttccgggccc
    79201 acgagacaac gcggctgaag ctgtattacg tgcggcaggc gccggaaaag tgtacgccag
    79261 ccatgcttat atgccatttg gcttgacggg tatcgctacg atcgcttacg aaatttggca
    79321 atcacttggc agagaacccg gaagcctgat ttcaccggtg ggtcatggtg gcttgcttta
    79381 tggggtaatg cgtgggttcg atgcgctctt ttcagccgga ttgattggca agaaacctat
    79441 ttatattggg gttcaagcag aaaactgcgc cccgctgttt caggcttaca ggcaacaaaa
    79501 actaaacccg gttaaagtgg aaatttctgc ctccctggca gagggaacac atgtttcttg
    79561 cccggtgcgg ggagcccaga tactggaacg aatgcaaaat caccaaggga gaatggtaat
    79621 gggctctgaa tcgcaactgg tagcgctcta tcggctctca gcacgatcgg gttatttttg
    79681 tgaaccgacc tcttgcttat cccttgtgcc gttactagat gataaaattg atcttgttga
    79741 accggttgtg gctatcatga caggctcagg ctataaaaca aatatcatct tacagtagaa
    79801 taatgtgatc gcgagctcct ctgaaaagag aggttggcgc tccccttatt cgtggagaaa
    79861 acgcttgact caaatgcata acgagtgtaa cagagaaacc cccgaagaac tggctgccag
    79921 aatctttccc gacttgaccg atatcctgct gttaagcaga gcattgaccc atcgctctta
    79981 cttaaacgaa catcctgagg ctttagagga caatgaacgc ttagaattcc tgggtgatgc
    80041 ggttcttgat tttgtggtgg gtgactggtt atataacagg taccctgaaa tgccagaagg
    80101 cgatttaaca cgcatgcgtt cggctttggt acacaccgct caactggcag attttgcgcg
    80161 tagcattcaa ttgggcaggg cgatgcgact gggcaagggg gaaacccagg cgggcggatg
    80221 tgagcgcaca gggttgctgt gcgacacctt tgaggcagtc gttggagcaa tctacctgga
    80281 aatggaggtt gaaggcgtca aacgttttat ctaccccatg cttgaatcct gcgctgatga
    80341 cattctgatt aatcacaaag atgaagaccc caaaagcaag ctgcaagaat gggcgcaagg
    80401 aaacggttac ccggcgccgg tttataccac ccgtgacgtt tcaggaccag accactcaaa
    80461 attctttgtc gtggatgtga tcgtaaacgg aaaaacctgc ggcacaggtt caggtaccag
    80521 caaacaggct gctaccaagc aggcggctgc agacgctctt aataaacttc acctttcaga
    80581 ttggaaacca actcagcgat gaaaccgaaa ctaagatcac tggagctgca tgggtacaaa
    80641 actttcgctg gcagagcgtt attcgagttc ccggggaaca tcaccgcgat cgtcggtcca
    80701 aacgggtcgg gaaaatcgaa catcgcagac gctgtacgct gggtgctggg tgaacaatcg
    80761 tatactttgt tgcgcggcaa aaagacagaa gacatgatct ttttgggttc tgaccaacgc
    80821 ccaaaagcag gaatggcttc agccacgatt acttttaaca acgaagaagg ctggctgccg
    80881 attgactaca gtgaggtcgc ggtcgcccga agagcgtatc gtgatggcag caacgagtac
    80941 ttgttaaacc ggcagcgagt gaggcttaaa gacaccacag aactactggc tcagtcaggc
    81001 ttggcggaga gaacctatac cattatcggc cagggcctgg tagacgctgc cttgtcttta
    81061 cgccctgaag aacgccgcaa attctttgaa gaagccgccg gaattggact attccgatct
    81121 cgccgagagg aagcgatcac caggttggat gctacccgcc gcaacctgga acgggtgacg
    81181 gatattctgg gtgagcttga acctcgtctg cgcagccttg aacgccaggc gaagagagtg
    81241 gaagaatatg accaggtgaa ggatgatctt caggtgttgt tgaaggactg gtatggattt
    81301 cattggcaaa atacacaaaa agaactgctg cgaaaccgcg aggtgttgaa aaaccaggaa
    81361 atcaaagttg aagccgccag gacaagacga ggagagatcg aagtaaaagt cgatgcggca
    81421 cgcttgcggc tgcaagaatt acgcagtcta cttaacgcct ggcatatgga aaccgctgaa
    81481 ttgcatcgtc agcgggaaaa aatcagtcgc gatatcgctg tactcgaaga acgtttccgt
    81541 gcatatcaaa accaggatat ttcactcaag gctgagttgt cacgaaatga agatttaaaa
    81601 cactcgaatc aagaaaaatt aacagcgtta atggatgagg aagaaaggat taaccttgag
    81661 ctcgcccagt gcgcggaaca gttggaagaa attcgtattt cttttgatga acgcaaagca
    81721 aatagagacc aactggaaac gaagataaat ttggcgcgca agcaactgtt ggaagttgaa
    81781 acccggcagg tgcaattaaa ggcgcatcaa cgcgaattaa ctggacgggt tgaatcattc
    81841 gaagatagcc ggaaaaatct gcttcagagt attgaccaaa atcagaacgg gcttaataaa
    81901 actcagaaag agatggaaga gattagcgct gggcaggtaa aaatccagca aagtgcagac
    81961 atatatgttc gtgatatcgg tatagctgaa gaaaaactgg ctgaactgga gcttcaggaa
    82021 aaagaatttt caggagaaat cagccgcctg gaggctgaac atgcccgggc gaaagcgcaa
    82081 ctggaagtgc ttcaccaggc agaactcgcg ttttccgggt tgagtgaagg cgccagaagc
    82141 ctgctcactg ccgcgagtca agggaaaatc cctgccggtt tacggaccct gagcagttat
    82201 ctggatgtac cgtatgaact tgaaactgca atgagcgccg cgttgggcga ccagcttgat
    82261 gcggttttgt ttggagaagg agttgacccg gagagaatac tctcctacct ggaaaacgat
    82321 gagtcaggcc gcgcaatgct gattccactc actgcggctg ggaaaggcca ggctcgaaaa
    82381 tttgctggcc ccgggatttt aggtgtcgct tctgacctgg taaaaccctc tgacctaaat
    82441 cgtggactgg tgaattttct gctggggagt gtgattatcg tcaacgatcg cgcaaccgcc
    82501 aggcggctgc taaaggattt gccagtggat ggcagaattg ttactctcaa gggtgaagta
    82561 tttgatggca gcggcgtcat cattgctgga aaagaaaatc gggctgggat tatcagccgt
    82621 ccgcgagaaa agaaagacct gacacagcag gtaaatcgtg ttgccgaaaa actggctgag
    82681 aaaaagaagg aactggaaga acttcaaaaa aacattatct ctttgcgtaa tgagaagagc
    82741 gagcttgata aggcagcccg ggaagagcag gctaaactcc aacagatcaa cgcaaccctt
    82801 gcgggaagag aacgtgaagt ggcgaaactc atgcagcagc tggaatggca aaataaccaa
    82861 atcaacacgc tggtcagcca ggccaaatcc gctgaaatgg atatggacgg gatcggaaaa
    82921 gagatcgcgg cgaatgagaa cgccatcatt gagctgaatc aaaccatccg gcaggataac
    82981 gaacagctac ggggcattcc gatcgatgaa attcagcaac aattggtgca ctggaacacc
    83041 acccaggcgg tgaccgaaag ggcgctgcga gaggtggccg ggcgagtgaa tgaatccaaa
    83101 cagaccatcg cccaaaatca agcggtcatt gaacaggtaa acagacgcat cgaagaggtg
    83161 gtgaccgcaa ccgctgaagt ccgggcttct atcgaggaaa gcaaaaaggc agaagggttg
    83221 gttaacttaa gcctggatga attcagtcaa aaaattaatc cggcagaaag tgaattgagg
    83281 aaacttgaaa gcgagtattc cgagttgcag catgaatatt cacagaccca gcaattcgag
    83341 gccaatgctg atcgggtata cacacaggcc cagttggagt attcccgcag ccgcgaagca
    83401 ttagaaggcc tgcggagacg gattgaagaa gatttcggtt tggtaaccct cgaatattct
    83461 gatgaggtca ctggacccac tccattgcca ttggaaggcg tgggcacact ggttgtgctg
    83521 gatgaacttc ccaagggact cgaagattcg ataaatcggc agcgcgcgct gttgaggaga
    83581 atgggagcga tcaacccgga ggctcgcaaa gaataccagg aagtcaagga acgccatgat
    83641 ttcatgaaaa cccaactggc ggatctacag aaggcggata gcgaccttcg ccaaatcatc
    83701 acagagctgg atgaactgat gcaccgccag tttcggacga cgtttaatga ggtggcagcg
    83761 gaattcaaag aaatgttctc acggttgttt ggtgggggtt cggcgcggtt agtgatgact
    83821 gatacagaga atccgacgga gacgggaata gatattgaag ccaggctgcc aggcaggcgt
    83881 gaacagggac tgtcactgct ctccggcggc gagcgctcac tcacggctgt cgcgctcatc
    83941 ttctcattac tgcgcgtttc acccacgcca ttttgcatcc tcgacgaagt ggacgcgatg
    84001 ctggatgaag ccaatgttgg cagatttact gagttgttga aagaactcag ccaggagacg
    84061 cagtttattg tcatcacgca caaccgtaat actgtgcagg tggcggatgt gatctatggg
    84121 gtgaccatgg ggcgcgattc tgccagccag gtcatcagcc tgcgccttga cgaactgagc
    84181 gacgaaatgg ttaaataaat tgccaaatga aaaccgcgag aatctcgtct cgcggttttt
    84241 ttttataatc tactgaccag gcgcaattcg atattgcgcc ggtatggcgg gggtattcgg
    84301 gacgaccgat gcccagacgt cttttttctc aattttgata tccgctttgg cagcttcaat
    84361 gaaatcagtg aaggctttct gtttcagtgt ctcccattgg ctgtctgaaa gagcgcggac
    84421 ttccttgccc aacaactgaa tcacgtgaaa accaaagtca gattgaacgg gctggctgat
    84481 ttcgccaatt ttgagggcgt aggctgcggt ttcgaaaggt tcgaccatct gtcctctaga
    84541 aaaccagccc aggtcgcctc cattgacgcc ggaagaggtg tcaatggaga gttcagcggc
    84601 gatagttccg aaatcttcac ctttcttcaa ccgttcgatc acagcattgg cttcttcttc
    84661 agacgccacc aggatatgcc gggcccagac catttcctgt tcgcggggta tatccttggt
    84721 cactgcttcg taaacctttt gatttaataa gaggcttttt acatacgcgc gaaaatccgc
    84781 ttcagtaaac gggaactgtt cattgatgct ggtgagcatt tgattgaaaa gctgtttgaa
    84841 accatcgcgg gtatacgcgg taggcgtagg agggatctgc gtggcggtag gcacgctggt
    84901 gggagtgcta tcttccactg tgggggtggc gagcgatgga tcagccgtcg cagtcggttc
    84961 acttgtggcg gtgggttccg gggatggcgt gggcgtaatg gtgatgatgg ccagctgctg
    85021 cgctgacatg gttgatgtag gcaggtaggc gtaggtaggt tgcggtgtgg gcgtgccgtc
    85081 tgggaagaaa ccgaagccac gttcaagttc cttttcgata tcttcatctg taacggagat
    85141 acccatttcg gccgctttgt gggccacaac cgcctcgtta atcattttct ccaatacatc
    85201 gctgccgaac tggatgtagt tgtccagcgc gttttgcatc tgcagcattt gcgattggaa
    85261 aaacatcgcg tatccagatg cggcgtaaga agtaaagttt tcaacataag acaagcgttc
    85321 atactgcacg cgttttgcga actggcttgc ttttatgttg gtatcgccta ccttcgccac
    85381 gattttttga ttcttaagaa cagtcccttg caacaccccg aaacctacca gcaaggtaac
    85441 cactgccacg atcgcgataa ggacggtgat catgatcttc ttctggcgat tttcacgctg
    85501 caacctggct tcacgctttt tagaagcagg ctttggtgtt ctctcgatgt tttcttttgt
    85561 catttgtcct ctatatctta ttcgagcgca atctgatgac gatgattgcg acgaaattgt
    85621 gaatagcagg tattaggttc tgcgttacct gatcgactcg ctccaacgtt caccggtgaa
    85681 cgggagaagg gcgatatggc gggcacgttt tacagctgat gccagggagc gctgatggcg
    85741 agcgcaggtt ccggtttgcc ttctggggcg gatcttaccg tcttcggtga caaagcggcg
    85801 taatagatcg gtgtttttat aatcgatggc gatattcttg tctgcgcaga actggcagat
    85861 cttcgggcgt gctacaaaac ggcgctgacc agaaaatcgt tcgctttcgt ttgtaatttc
    85921 gtctgacatt ttttactcca ttagaacgga tattcatcaa ccgcttgaaa atcggtttcc
    85981 tgaaggtccc cgttgggttg ggcattttct ttcttctcgc ccaggatcat catttccgaa
    86041 gccactattt ccacattgct gtgtttgact ccttcggaat cttcccagcg gcgggtttgc
    86101 aggcggcctt caacataaac ctgttgaccc ttagtgagat attgtttgca tatttcagcc
    86161 aggtttcccc aggagaccac atagaaccat tcagtttctg agtggcgttc accatctcca
    86221 gtattccagg aacggttggt ggcgactgta aaagttgtta caggtcggcc ggaaggggtg
    86281 aaacgcattt cggggtcgcg ccccaaatga ccaatgatca taactttatt taaatcacga
    86341 cccattttct aactcctcat tcttcctgca gcttttatta cgctaccaca gtgatcatat
    86401 aacggatcac gggttcgtga aagcgcaggt tacggtcaag ctcagaagtg agggcggggt
    86461 ccatttctgc agtgagaagg aagtactgtc cttcgcgctg tttgcgaatg aggtaagcaa
    86521 gacgtttctt gccccagtta tccactttgg atatactgcc gccgcccgct gtgatccagc
    86581 cttttacctt gtcgagagcc tcattgagag cattctcgtc cagttccggg tggaagatca
    86641 atacgagttc gtatgtgcgc atggtgattc ctcctttcct cgggtttgcc ctcgaacagc
    86701 accgttggtg cggtcaagag cggaaaggta gcagtaactt gctgcgtacc agacgtagta
    86761 ttttaccata agttcttcga aatctaaggg aattaatccc gtcatcttgc tgggtacaaa
    86821 gattaagatg gtgatttgaa acgaaaccaa caggatagct ggttcaggcg tgagagcgtt
    86881 ctgcgagcga gtattaatag tgggatgaaa aaaagtgatt ggtcattctc actattaatg
    86941 agcgcttcca attattgttg atttaatcat tcgttcgctt aaagacagga gaaaccagga
    87001 aatgagccgt atttatggta aaatactgac cgttgttggg ggctcgtcta acggcaggac
    87061 agctgactct ggatcagtaa gtagaggttc gaatcctttg cccccagcca aagagagaag
    87121 ggtcttcgaa gtcgaaggcc ctcaattatt ttggtgctgg aaaaagacac tgtgtatctt
    87181 cttgcatctc tgtaaaagca tgagcacctc aatagagggt tttagtgatt cctttatgga
    87241 ccaacatcag aaatcaattt gaccagcgcg ttataccttc tggaccaatc atgttgtctg
    87301 gcaaattgga tgtaagtatc ggcgcgctcc tttattggct gataattcat caaagccgtc
    87361 aagaattctt catatgaact gcataaaagt gccggcgatg cgatgtgttc taattcatcc
    87421 cattttgatg ccaccactgg taaaccacag gccatatact cgtaaagttt caccgggcag
    87481 atcgcattga ccaaagtggg gaaatcccgc tgattgaatg gaataatgcc gatatccgcg
    87541 taggaaagat aggctggcag ttcagaatga ggaactggac cggccaaaat gaggtttttc
    87601 actttgataa atcgcgaacc gaaattctga ttgggaccaa ttatgacaaa ggtgaatttt
    87661 ggcagggcaa tggctaaacg attgataaga tcaaagtcga accagaaatc aattgagccg
    87721 gcgtaaacga tcaaagggcg ggaacaagat cgatagagct cagggatggc tttcactttt
    87781 tggaaatgtt cgaaatcaac tccgtttggc aggaaaacgg cttgcttggg ttgtaattgc
    87841 ctgacacgat tttctaattg atgagcagaa taagcaacca gatcaacctt ggaggcgatc
    87901 ttatgctcag cttgtgtata aaaagggttg taggaagaaa agccagagtc gttatctgcc
    87961 acccgaaata ctttcttttt ggcgttgatt aaatccagca aatacccctg caaaggatcg
    88021 cgaatatgga gcaaatccac atccaaaaaa ccttttactg ccaggttttt cttaatgttc
    88081 gggaaaattg tcttgtgcca gtactgaaaa acccattttt tcgccaaaaa aatctcgttt
    88141 ttcggaatca ccgcggcaaa tggtacaaaa gaacagatta tgccttttcc atgcgtatac
    88201 catcgcccat tggacaggca ggtggaaaac cgccttttaa acgaattatt gtctcttgca
    88261 accaaatgcc aaggggtgat gggtttggag ataaaaatca cttgccaacc attggacgca
    88321 aattgcctgg ctaattgatg gtcgcctact tgaataggcg actcataatc aacagacgta
    88381 ataaacacgg cttttcgaat ggggtctgtc atctcattac gcgttctgcg ttctcaataa
    88441 aaactggtga taagttgatg cattggactt caggtcaacg agatccttat acttaaatta
    88501 catacctgta acaccgtctt cgatcaattt acgggtcgcc gaagaatcgg aggtaatagc
    88561 aggtatccca taccttgcgg ctttctgaac caccgatccc tgtgcttcat accatctgga
    88621 aggaaaagac aatacgcgag taacgcttat tcttttctga atctttctga aggataacca
    88681 gccttcgaag attgacatgg gattaaatcc tctgtaatac ggacattaag gtgatgatac
    88741 ctactggaaa gaattataaa agaaaagcct ttaattcgag cattttttca tcctttgcgt
    88801 ttttgaaaag cttcagacac atatgagaca aaaaagagca tcgagttctt ccttcacggg
    88861 gacaatccaa taaaaggaag agtctcaagg aaaagaaagt aagtgaaaag gagatcaacc
    88921 tgctgctgag aggatcaggc tcgaccgtgg ggcgaatacg ggtagaccag aggagaagaa
    88981 gagaaaaaag gagcaaaagg agggcatgtg ccatacgaaa accgaagaat cgtgtggtca
    89041 atgaacctgg cgacctggtt caggcgggcg cttcaatcat ctgtaaaagg gaacgcgtct
    89101 ataaagggac tcgccccatc aggcactgga ctatctcatc ccggccgagt atatttatat
    89161 gtaccacccc agggtgactt cgaaaaaggc caatatgaat tgaacaagac caaccgcttg
    89221 tcaacgcaaa acaagatatg gagggatgga atgaaaaaac tggcgattgc attaattctc
    89281 ctgctggcta cccctgcttg ttcgatttcg aatcaacatt ctgtggagat tcaaccgggc
    89341 ttggatgtat ctacgccagc caaggaggtg ccgccacctg cgccgcaacc gcttccaccc
    89401 gcgcaactca gtggcgattt ggtcagccct gacgactttg agtaccttgg cgcgttccgc
    89461 ctccccggtg gtgaagaccg cccgcagact ttcgcctacg gcggcaacgc catgacctat
    89521 aaccctgacg ggaactcgct cttcatcatg gggcatgacc gtatgcccta cggcgacctg
    89581 ccagatggga atcaagtagc ggagatcagt atcccgaaac cggtcatttc caaaaacatc
    89641 gaagacctca acacagcgga tttatccaaa aattttcaaa tgtgttgaag agacaattca
    89701 gcgagtacga cgaaatcccc aaggctggaa tgcagtacct cagccgtgcc gaaacgggag
    89761 ccaagattca tgttgcgttt ggcgagcacc tccagtcgga gcaaatcccg acgcatggct
    89821 ggttcagccc tacgttgtcg atccccgatt ttcagggaac atggttcatt gggaaccagg
    89881 acctgtacag cgtgaacggc tacatgttcg aaatccctgc tgaatgggca gatacctgca
    89941 ctggcggaag accccttggc actggtcgaa tgcgcgacgg tggccagggt ggcatggggc
    90001 caacgctctt cgcttatcgc ccgtggaatg cggatggctc tcccttgcct gccgggaccc
    90061 gtctcgatga agttaccttg ctcttgtatg agaacgccta caacaccgag gaaatagtcc
    90121 gcagtatgga cggctaccag catccagatg aatggggcgg tggcgcatgg ctgacaactt
    90181 caggcggcaa agcagcagtt ttatttgcag ggacaaaaag caatggcgaa aaatactggt
    90241 atgggtacat ccaccccgat ggcgcgaacc tcgcctgtgt ggatgcggaa gtcacggact
    90301 tccctacctg tcgtatggcg gatggttcag tctgcccgca ggaggattac actgcttgct
    90361 gtgatgccgt tgcgggagaa tgtgtctcca accgtggttg gtggacaacc cgcttcgacg
    90421 ccgagtttat cctctatgac cccgctgatt tggctaaagt cgctaccggc cagctggaac
    90481 cgtggcaacc acaaccctac gccgttatcg acattgacga acacctcttt ctgagtccgc
    90541 ccgaatggga tttggtcgag ctcggctgga gtgatcagcg ccgaagccgc attggagatg
    90601 tggcatttga ccgtcaaaat gggttgcttt atgtgcttga gttgtacgga gatggcacaa
    90661 aaccagttgt gcatctgtgg aaggttcatt agattaaatg gcgtatttac cctgaagagg
    90721 gaaattcaac tccaggttaa agaaactggt tgcagatttg cgattggaca atgccatgcc
    90781 gccaggcagg ctggggaagc cctgatgccc tggagtggaa agcctgcgat gtgatctgcc
    90841 agccacgata cctaacgata taacaattag atcccagatg gtgaggaact gcagcgagga
    90901 agagtgtggt tcaatgatgg ttcgttggtc aggtggaaat agttctttca agtcaagtag
    90961 cattgaatta taataggcct aaccgtaaaa attggtttca actgaactgt atcaaaagca
    91021 attattttat gttcattaat caccttaata acccaatatt gacgccagcg acaacgatat
    91081 actcccgtct acacggtaaa aagaaaaaac ataatggttg cagcaagctg gagaggttta
    91141 attttttggc acgatcttaa aaatccgcac ccagcagatt taattgaagc gacattcgcc
    91201 ctccctgttc ggggttcgct gatttttatc gccaggttga aaaccccgta atacagcgat
    91261 atggacacca gcgaagcgct tttgctcttc acgaggattg gaagtgcttt ggtggtaatg
    91321 gaaaatcgct aaaggcaagg ttttgaaaaa aaattctttt cttatgtcag aataacgtgg
    91381 ctcttaaaca gattcgctcc ttttttcgcg accccgagta ctatcgaggt ttgctgcgat
    91441 tgatgctgcc cattgcgctg cagaacttga tcacatcctc gctaaacttt gtctccgtgg
    91501 tgatgatcgg gcagctgggt gaagtttcca tcgcttctgt tggattggga aaccaggtgt
    91561 ggtttttgct caatctgatg gtttttggca tttccagtgg ttcagcgatg ttctctgctc
    91621 aactttgggg aaaaaaggat gtgcacaata tccggcgagt ggtaggtctc accgtcaaac
    91681 tggggttggt cgctgccgcg attttcttta ttcttgcacg tttctttcct ggagccgcct
    91741 tacgcgttta tagcaacgac ccagaagtga ttgccactgg cagccgatat ttacgcatcg
    91801 tttgctggac ctatggtttt tacgctcttt cagctgtatt cagtatggcc tcgcgcgcgg
    91861 tagggaatgt acgtttacca gtggcggtaa gcacctcagc tctcattatc aatgtgctca
    91921 tcgcttatcc gctcatcttt ggcgtcagat ttttgggtat ccctgcgttg ggcgtagagg
    91981 gagccgccat tgccggggtt atcgcgaggg tgatcgaatg tggcgcgtta ctgtttttca
    92041 tataccgtaa tcgcgaaaac cccgtggcag cgggaccgcg ctacttgatg gagttggatt
    92101 gggaattcat caagcgcgtt atggttccag tgctgccggt cattggcaat gaaatactat
    92161 ggtcatttgg aatcacgacg tacaacgcca tttacgggca tattggtacc aaagcggttg
    92221 cagcgatcaa tatcatttcc acagtggacc aggtagcctt tgtggtattc ctggggttgg
    92281 gaaacgctac cgcagtcttg gtgggtaact tgatcgggca ggggagaatg gaaaaagcat
    92341 acctttacgc aggcaggtct ttgatactgc aggtgttagg ggcggttgta atgggattac
    92401 tggtcatttt attcgctcca ttgatccttg gtttttaccg tgttgaccca gaggtgatag
    92461 tgaacgcgcg caggctattg tggatattgg gcttgggcct gggggtgcgg gctgccaatc
    92521 atatgatagt gattggaatc ttgcgctcag gtggggatac gcgtttttcg ttagtactgg
    92581 atggattggt gatttggctg gtgggagttc cgtttacagc tgcaggcgcg tttctattcc
    92641 atcttcctgt ttacctggtt tacgcgctta cgttgaccga agagattacc aaagcctcgc
    92701 ttgggctgaa acgttactta tcgaaaaaat ggatcaatga tctcacaggt cgtgtatcag
    92761 agatcatccc tgagtgaaac ctgctggttg ttcatatggt tctggttttt tggggtcatt
    92821 gaccagcagt atcagtatgc cgctggcaat gaatgccatc accgccgagg tgataaacgc
    92881 tgtgacgtaa tcgaacgcat ccgccagaag gccgccaatt aatggggcga taatcgccgc
    92941 tggcgccacc agggtattgg ccatgcccac gtaggtgggt ttttccgctt cttctccaaa
    93001 ttcaagcgac atggtaatac caatcgtcca aaaagctgta ttcgctgcgc cgttgagtaa
    93061 aaagaccagc gcgaagagat ttacctcctt aatcaatacg gcaagtaaag cgctcaccgc
    93121 tgcgcatacg ctccctatca ccaacaccca ttttcgcgac catcggtcag atagtcgtcc
    93181 cagtaacggg ttcattacca tcatggtgac catcaaaata ctggtcatca cccccacttg
    93241 aacagtggtc attctaagaa ttttcactgc atagactgag tagaaggcaa acgccatcat
    93301 accgaaatgg gcaataaaac ggctgatcag gaagtgagta aatgaacggt cgcgtttcag
    93361 gatggaccga atgtcatgcc aaagaggttg gggaacgcaa ccgccggatg gcatttctct
    93421 tttttcttcc ctggtgaggt ttagaaacac ccaagaagac acaagtaaaa gggaagcaat
    93481 gaaaaaacag gtggaaaagt cgtagggagg gttgagcacc accagcaggg ttcctgccag
    93541 gtatgcaccc aggcttgcca gcaggtttgc tgctgccgat tgcgcaccaa aaaaagtcgc
    93601 caggatattg ctgggaatga ttttgctgac catgatttgc caggcattag ccgtcagtcc
    93661 cgcgcccaac ccctgccata taagcaatat gaaggtaaga atgagcgcta ctcttacacc
    93721 aatgcgaggg acaagcagcc cgacgattcc cagccctaaa atcggcaatc tttcctgaac
    93781 ggtcataaag gtcacatagg gcttaacacg ctccatcctg gcaatgcgtt tcgccatcaa
    93841 caattgtgga agctgccagc cgacgttgtg aatggctggc acgagcccga taagtgtggc
    93901 agaagaggtg agcgttgcca caaaaagggg gatcattgtt gtgaacgaag cgaaacccaa
    93961 accaaagccg aacaaacctc catcgaggag gttgacagtg aagttgtgtt tcaagttttt
    94021 gctttgttgt gagtcgctca tttcaggcaa gttaaggtaa accccgggtt gaccggggtt
    94081 tgatttttca atcaacagaa aagtgattaa tcctcatcgt cataatccaa ctcatcccag
    94141 tcgtcttccc cttcctcttc catgtcttcc cattcttcca gctcgtcttc ttcggcgtcc
    94201 caatcttcag tgcttaatgc ttctgcactg actgtgtatg ttttacaacc agtatctggg
    94261 tccagttcaa ccattccagc cgtgcatatg ccttcatcca ggaatgagca atcgaggtag
    94321 tggcatttga ttcttggcat attattttcc tcctgatgct gtagtcgacg gcgattttac
    94381 tacaagtctg ataattgtgg tgataaaaat cacactcatc atcatggctg acaacaggca
    94441 ccattgacaa accgttttca acacaaaaat ctcgatgctg gtgagataca gggaaaatag
    94501 gaaccccgcc aggctgaatc caaacaggat cagatcgatg taagggtgat tcgcgcgttg
    94561 gtgtaaacca aaagcagcga ggaggaagat caggccaaag ccggcaacgc cataaaaacc
    94621 aagcgggata ccaaacagta ttgaccaacg gctggcgttc accacatcac aatgacccaa
    94681 tcctggcgtg cagtaaatgg gggttttcgc gatctttaca tatgcgaggt agatggagtc
    94741 cagcaaaccg ataatggcag aaatctggat gatgagggtg ctcgttcttg gacgtgaaga
    94801 tttcatttgt caatcatctg ttttcagagt gtcgggcatt ctacaatgaa taggagatta
    94861 agtaaaggtt aatcgccagc acagggcacg gagaagagac tattgaatgt gtttatccgg
    94921 ggttcaggcg atgcgcttcc atgacattcg gcaggttttc aatgagggtg agaacgcgag
    94981 agagttgcac gatgtcagct atttccagtt tcattacaat ggatgccagg ttgtgagaga
    95041 cattgatttt aatgtccagc aaattgatat tttcgttgct gagcaccgag gatatatcga
    95101 ccattaatcc ctggcggtcg taggctttaa tttcaatttc caccgggaat gtgtgctgcg
    95161 gttcacccca agccactcgc acaatgcgct cccggtccgt catgcgtaat atattgggac
    95221 aatcttgacg atggatcgtg gcgccgcgac cacgggtgat gtaccccaca atggaatcac
    95281 ccggaacagg attgcagcaa cgcgcgaagg tggtcagaat attgcgcaac cccagcacag
    95341 acacggcgtt ctcagaaatg tctttcgcct gggtggatga cttaatctcg aacaggcgag
    95401 actcgtcagc ctctccttct gacagggtgt tgatgatatg cccgagtgac atatcgccac
    95461 agccaaccgc cacatacatg tcatctaagg tgcggtaatc gaaaccgcgg gaaagttttt
    95521 ctaaatcata ttgttgcagt cccagccggc gcagttcccg ttccagtatt tctttcccct
    95581 gggcaaggtt ttgttcacga ttctggtgtt tgaaccaggc gcggattttc gaacgcgccc
    95641 gttgcgtttt taccaagccg agggtggggt tcaaccagtc gcgggaaggc ccaccttgtt
    95701 tagccgccag tatttccacc tggtcaccgg tgtgcagcgt gtaatccagg ctcacaagct
    95761 tgccgttcac ttttgcaccc cggcaacggt tgccgatctc ggtgtggact gtataagcga
    95821 agtcaatagg agtagcgcct gcggggaggt caataatatc gccgcgcgga gtgaaaacat
    95881 aaacccggtc ctggaaaaca tcggttctca tgccttccac gaactcattc gcgtcttcca
    95941 cttcctggcg ccattccatc aactttttca accagttgat acgctgctca tattgctcat
    96001 tccgcgggcc tttttcttta taacgccaat gcgccgcgat tccgtactca gcgttttcgt
    96061 gcatctcagg ggtacggatc tgtacctcga gcggctttcc atcgtcatac agaaccgcag
    96121 tgtgcaggga ctgataaaaa ttatcttttg gcgcggcaat gtaatcatca aattcgttgg
    96181 ggactggccg ccagtgggta tgaatgatac ccagaaccgc atagcaagag ggaatatccg
    96241 gaacaatcag cctgaccccg cgcaggtcgc gcaccagttc aaagggtttg tctttctcaa
    96301 ccatcttccg gtagatcgaa tagatatgct tgctgcgccc tgatatttcc accttgatgc
    96361 cagcctcatt gatcaatttt gccattcggt cgatgatggc tttcatctgg cgctcacgct
    96421 ctaacctcgt ttcagccagg ttctgggcga tttctttata cttctccgga ttggtataac
    96481 ggaaccccag gtcttcgagc tcccatttca attgccagat acccagacgg ttcgcgagcg
    96541 gggcgaagat atccagtgtt tcctgtgcga tacggcggcg tttagcttca ggcacaaagc
    96601 cgagagtgcg catattgtgc aggcggtcag ccagtttaat caagataacg cggatatctt
    96661 cacccattgc caagaaggtc ttacgcagcg tctcattggc aaggtcacgg cggcggtcgc
    96721 gggcatgctg cgagttcaac agcatttcct gttcttcctg gttcttttcc atgttcttgc
    96781 caaaaatttc agcgtcgtgc tggtcggccc gtgatactcg cggcaggtgg gtcaacttcg
    96841 tcaccccatc cacgagcttg gctacctcat cgccgaagtt acgttgaatg tcttgaagcg
    96901 tgacgctggt atcttccacg gtatcatgca gcaacccagc agccaccact actggctgaa
    96961 cgtgcatttc agccaggatg atggaaaccg ccacgcaatg cgtgatatag ggctctccag
    97021 aagctcgttt ttgttcggcg tgcgcatttt ccgcaaagtt gtacgcctga atgatgaact
    97081 ctctatctac tctggaataa ttgtccggca gaatatctaa aaggttttct agtaaggggc
    97141 tggttttcgt tgtactgacc atgttcgact cttgttaaac tcttagtata tcacccgaca
    97201 agaaaaacca gaccttttcc cttctttgtt gtgcagaacg gagatccacg catacccata
    97261 cgcccgagtg tggcgtactc ttccaactgg gttaattcat agaaagggca agttacttta
    97321 atagttcctg gccttcaaat tctcgttgtg gagaaagata gcgcagtgta tcagcgtctt
    97381 ccgggagtac atcgccggta acagcgcggg ttagcagttc gccgagggct gggcctaaca
    97441 taaagccctg accgcacaac cccactccga gaagcaggtt gctcacctgc gagctccagc
    97501 cgacgatcgg gaacccatca ggcgtcattg gatagagacc ccgccatgtc cggcgcacgc
    97561 ggatgttttt cagcctgggc atgatggaaa tcatccgcct ggcaaccatt ggcagaaagc
    97621 tactggtttc gttcacatct tctccccaga tatttgggct gggagtgatg cagaaaatca
    97681 cctgaccggt ggtgtgctgg taaaaatagt aatttgatga accaggtgcc ggccgaatgt
    97741 caacgatcat agggccgagg aacctcgcca ccggctctgt gatggctgcc tcgtggctgt
    97801 cggcacgaac tggaatttca acacccgcca tgcgtccgat ttcggcagcg gaagcgccgg
    97861 ctgtgttcag cacgacaccg gctgaatagg tttcacgact ggtggtgact gaaataactt
    97921 tcccccgctt cacaaccatg ccggtgaccg gttcacaaaa atggaatgta actcccttct
    97981 ctacggcgag ggtgaaataa gcgtgattgg ataagagcgg cgaggcgctg ccgtcgttgg
    98041 gggagaaggt gccgccaagc aggccctgag gagtcaggtc agggatgatc tttaacaggt
    98101 catctttcgc gtaccagttg ttcaccaatc catacgtatg ctggatggtc agcaagtctt
    98161 tgagaatctt ctcttcacgg ggagtgtagg caatgaaaga gtacccacac tgatcccact
    98221 cgatatcttt accgtaaact tctttgaagg tggaaaagat ctcaatgctg cgcaggcaaa
    98281 gacggatctt agccggatcg gaatgggtgg cgcgtatacc gccgatggcg cgtttgtttg
    98341 atccctggcc aacgctgggg accgtttcaa gcacaagtac ttttaaacca gcagaagcga
    98401 aataaaaagc cgccggggtt cccagtgaac ccgcgccgat aatgatgacg tcgtatgtgt
    98461 tttgggggtt cattggttat caccctcccc gttttcgccc gcgaagacgc ccaaacctac
    98521 ttccacaaac aacggacggc gggagggatc ggtgacttgg tcgtagggca cgccttcttc
    98581 tctgaagatg cgatggatca aaggtgcaca ggtcttggct ccgcaggcgc ccatgcaggc
    98641 acgggtcacg gttttaattt cattgatatc gcggtagcca tttcgaatgc attctcgaat
    98701 ttcgccggca gttacatgct cgcagcggca tacaatggtg tcgtctgtga catggttcac
    98761 atactgcaac attggttgcg tgatcgcggg ctgctgcatt ctaattcccg cgatgtgggg
    98821 tgcgatgttt tgctctactt ccacctgaat cacaatggtt ctgtcgttcg ctttcaaggc
    98881 gcgaatgttc accacttcag ccgtaccaag ttcatctccc tgggtgtctt ccagggtaac
    98941 ctggtcgccg atatggatgg actcgcggga gaactcataa gggatggaca ctaaaggtct
    99001 ttcgctactg ctgcggtaat ctaccagggt gatcgccaga cccgggcagc cggcaataca
    99061 ttttaaacag gctttacagg cgaagttatc ctggacaaat tttggcgttg aacggatatc
    99121 ctgctggtcg ataaagatca agtgttgagg gcacaaagtt gaacaagggt cgcagggaat
    99181 ttcttggcta cagtggaaaa ccggcagcac gccttccggc aggtcgggca ttttttcact
    99241 gagcacattt cctggtttag atttgaggat ttcgcccatg cggtaccatt ctgcagggat
    99301 ttcaccagtg tttagaccca aatgatgagc cacttcaagc cctttgatct tgccgctaaa
    99361 aatggccgcg gatgcttctg caatctcttc agcatctccg gcgacaaagg cagggatacc
    99421 aaagtcattg gctttgtgga agaattcatt cactgggtca agaccgaccg cgatgagcac
    99481 agaatcgcaa gcgtacgacc tttcactccc tggtactggt tggaattgct catcgaccct
    99541 ggcgatggtg accgattcaa catggtcttg tccgttggct gagaggatgg tgtgcgaggt
    99601 gtaaattggc acgcccattc gcaccagttt atctttatgc actttataac caccacattg
    99661 tggaagggct tcaaccaatc cgacaacgcc aatcccggcc tgaagcgcgt gataccctgc
    99721 aatcaagccg acgtttccac cacccacgat gaacagcttt tctgccggca aggcaaggtc
    99781 gcggttgacc agcgtttgaa atgcaccggc gccgaagacc cctggcaggg tgttgccttt
    99841 aaacgcgagg aatttttcac gtgcgccggt cgccaccagc aacacttgag ggcggataag
    99901 aacatagtgc ttgccatctt ttaacactcc taccttctga tcgctgaaaa cagccaaaca
    99961 agtggtttgc ggccaaattt gaacagaagg gtacgcacgc acttcgtttt ccaacctggt
   100021 ggcgatgtct attccacggg tgccggcgta aaccgctttg gaggagccaa aaaagcggtg
   100081 ggtttgcagc accaacttac caccaagacg gtgcttatca tccaccagta taacttctgc
   100141 gcccagcttc cccaattcaa tggctgcgga tagaccggca gggccgccac cgataatcag
   100201 caccggaatg tcgatttctg ggattggctt taattcaggt actacgctta cttcaggcag
   100261 acgagggtga ccgtcgtttg gataaacccg catcccgttg gtgacaatct ccatacagga
   100321 ttttaaaggt ttgccatccg cgattaccat acattgcgcg cactgaccgt tggcgcaaaa
   100381 aattcccaaa ggtgaaccat ctttgtgatg atgcccgaaa gtgcgaatac cgttggcgaa
   100441 aagcgcggcg gagatggttt cacccttatg agcggacagg ggtttgtcct gccagaagaa
   100501 ctcgatcatt tcaggttcag ggatgggcaa gatgggatgc tgcttgatac ggtaaccggt
   100561 catggattat cctcagtata cggtttgggg tttctacccg tagaacgaga tgggaactca
   100621 agcgttttta attttaccaa atagtagaaa acaggcgcat cggttttttc gggtatttgt
   100681 aacctatgat tattggagga ggaaaagccg ggtgcacgcc acctggtatt gcgcgccctg
   100741 caggtcctga aggctatcgg agcgtaaacc tgcacaaacc gagtgagaag gaaagcaaga
   100801 gggtgttgtg ttcaaccagt gtctgcaata taatgaattt cagataatat cgaacaattg
   100861 aaccttggag ttctagggag agacccatga aacgcattct gttttcgctt atcgcggcac
   100921 tcttcctgct cggggcagcg ccgcagcccg ctccgacgat gcaggctgac aaccccaatc
   100981 cgccaactca gacggtaaaa ctgattttca tccatcactc cactggagag aactggctgc
   101041 gggacgacaa cggcggcctg ggcatggccc tggctcagaa caactacttt gtcagcgaca
   101101 ccaactatgg ctggggaccg gattccatcg gcgacaggac cgacatccct aattggacag
   101161 agtggtttgc cagcgaaaac acgccgacca tcatgcaggc ggtctacaac gaaagtaggc
   101221 agaactcgga gtatactcgc aaccggcctg accccggagg ggagaatact gtcatccttt
   101281 tcaaatcgtg cttccctaac tcggcgctgg agggcaaccc gaacgatcct ccctctgagg
   101341 agggtgacct gaccgtgggg catgccaagt gggtctacaa ccagatcttg ccctacttta
   101401 aaagccagcc tgacaagctg ttcatcgtca tcactgcccc tccgctatcg gataatacct
   101461 acgctcaaaa tgcccgcgac ttcaataact ggctggtgaa cgactggctg cgagaaaaca
   101521 actacacgct caacaatgta gcggtctatg acttttacaa tgtgctcagc gcgccggaca
   101581 atcaccactg gtataacaac ggccgggtgg aggacgttat caacaaacag cgcaacacca
   101641 acgcctaccc ctcgggggat gatcacccca accaacgggg taaccgcaaa gccagtgaag
   101701 agttcgtgcc ggtgttaaac gtgttttaca accgctggcg ggcatccgct cccgcccagc
   101761 ctccggtaca ggaggaggtc caggcacata ctggtcagga tgggcaatct ccgcttcagc
   101821 caggcagcca gccggaaggc ggcgggttga tcgacgcctt tgaaggcggc acgccccctg
   101881 gaacgaccgg ctgggagacc taccgagacg aaaccacgcc aagtattatc acctgtgccc
   101941 ccgacagcag tatggcgcat agcggaaacg cttcgatgga gatggaattt ttcgtaccgg
   102001 cagacagttg ggtaacctgt atgctttaca atgagcaacc tcagaactgg agtggacaac
   102061 aggggattag ctttttcatc cacgctcagc aggcaggcac agtcatccac gtcaacctct
   102121 acgcgggcga cccggctaac caggaatcac acgtttacgt gctggaaaca ccgcctgata
   102181 gcggcagcgg ctgggctttg atcgagatcc cctggggtgc gttccatcgt gtggagtggg
   102241 aggagaatag tggcgtcccg ttcaccctca ccaatgccgt cctggcgatc ggttttggcg
   102301 cgtccgccaa tccagatgag aataacaagg gcaccgtttg gattgacgac ctgggctttt
   102361 tgggcagcca ggttggcggc cagccgacga ccgccgccga gaaccctcag cctatcaccg
   102421 cccaacagca agaacagccg cgcagcgggc ttcgaaggtc actgccctgc ggcggttcgg
   102481 ttgccctgcc gttagggctg gttatcgtga gcctgctcaa acgcaggaaa cctttccgga
   102541 aataatccgt ccttcttatt acggcttttt taatataaca agatcaggaa ttcttcacca
   102601 tggatacgat gttttcgtag ggctctgggt ctcgcattaa gaaaaagttg aactgtatga
   102661 aattctctcc ctggtacatc cgtgccctga tacctcagct actcgaagat gtgcactatt
   102721 gaataggata ataaaaggcg aggattgaat atcttattat atataaagcc caaatatgca
   102781 aagggtagaa ggcatagaat aaatatttca aactgtgagg tccgcgttca ccattataaa
   102841 ggatgataaa cggtaaagcc gcaatcatca tccattgata ctcaaactcc cagaagtact
   102901 cgatattatt aagaccccac aagaaaaaga gaaggcacaa acccgcatag gcaaaatata
   102961 aggcaggttt tttgttgtaa aagaaataaa aaacgataaa cattgcaaga ccgttatagg
   103021 atgattctgt taaaaggctg aaaaacgcca aaattcctgc aaaaacaaaa ccaccccatt
   103081 tttttatccc gctttgtttg cgcccccatt caaatgcggc aatcatactt ataccgatgg
   103141 caatggatag aaaaatgttc tgccctaaag agccttcggc atgatacgca ttttttacaa
   103201 tttcgctaat gataagattg cccacttgca tggccaatgc ccatgcaaac aaccgaataa
   103261 tataacgacg gcgattactg gttttaaagt aactttccac ggaaaggaaa aagaaaatcg
   103321 gggcaacaat gcgcccagcg tatgtaagat aaagcggaac atgtgcaggt aacaattcgc
   103381 ccaaatagag accgaaatgc tctccgaaca tcagaacaag cgcaatcaat ttgagggcaa
   103441 agccatttaa tttcatcaat tctccttgat tatttctgaa ccaggcaccc gacgagactg
   103501 ccgaaaaacg atctctgaat ttttgaactg tgcaggttct tcatttgcaa gttggtctta
   103561 tccagcttgg ttttactcca tcttgaacag attcgattat ccaagccgat agtgatagga
   103621 ttgaataaaa aaaccgccgt tactcttttt tcatgcgcaa atacgcttca ataaagccgt
   103681 ccaggtcgcc gtcaagaaca gcgagagagt tgcccacttc gtagccgctg cggtggtctt
   103741 tcaccatctg gtatggatgc agcacatatg accgaatctg gctgccccat tcggccttct
   103801 ggtatgcgcc gcgcagatca gaaaggtgct ggtcagccgc aatctgagcc aggtcaagca
   103861 agcgcgcccg cagcacacgc atggcgtttt cgcggttttg catttgagac cgttcattct
   103921 ggcaggtgac cacgatcccc gtgggtagat gggtaaggcg cacggcagtg gcatttttct
   103981 gcacgttctg tcctccggcg cctgatgatc tgaaaatatc gatctttatc tcgtcaggat
   104041 taatgaccac ggttgggtct tcttgcaccg cttctggcag tacttctacc tgggcgaagg
   104101 aagtatggcg gcggtgggcc gcgtcaaaag gtgataacct caccagccgg tggacgcctt
   104161 tttcagcccg caggtatcca tatgcaaaag cgccgttcac tgcaatgctc accgttttaa
   104221 taccagcctc ttctccttca gtttgttcca gaatctcagt ttcaaaacca cgccgttcac
   104281 accagcgcag gtacatgcgt tcgagcatgg cagcccaatc ttgcgcgtct gtgccccctt
   104341 cacccgtgtt gatggtcaag agcgcgtttc cccggtcata tttcccgccc agcagggtgg
   104401 ccatttcaag gttagcgact tggttggtga tggcggttaa ttccgcttca agttcttgct
   104461 gcagcgaaga gtcagcaagt tccgccagtt ctaacgcatc actgatgcgc ttctcgaggt
   104521 tcttccaggt ggagatttca tctctcaaat cagccaggtt cttcataact atttgcgcct
   104581 gcctgggatc atcccaaaga ttgggatcct ggcttttcag tgttaaagcg gcgagttcat
   104641 tttctttttg aggtaagtca aagacgctcc aggagagaat gtactgtcgt taaattggct
   104701 tgcaaggtgg caattaattc ttccatgaat gaaccctact cttttacccc aaggatgttt
   104761 tttacgaatt gatagcggtc gaagcgttgt aaatcttcag gcttttcgcc cagacccgcg
   104821 tagtaaattg gtaatccaag ctcgctttga attgcaaaag ccattccacc gcgggctgaa
   104881 gtatccaact tggaaaggat gaccccgctg acgttcaccg cctgttgaaa ggcgcgcgcc
   104941 tggtgaaggg cgttttgtcc ggtcgtggca tccagcacca gcagggtggc gtgtggcgcg
   105001 ccaggaagag ccttggcaag cactctgtgc accttcttca gttcttccat caggttataa
   105061 cgggtatgca ggcgccccgc ggtatctatg agcacgatat ccatgttccg cgcgatgccc
   105121 gcctgcagcg catcatacgc tactgctcca gggtcgctct ctggttgacc ggcgatgact
   105181 ggcaggtgga ggcgatctcc ccaaacttgc aactggtcga tggcagcagc gcgaaaagtg
   105241 tccgctgctg ctaaaaggac cttcttccct tcatttgagt agataaaacc aagctttgcc
   105301 gcggttgtgg ttttccctga gccgttgacg cccaccaaag tgaccacagc agggtggtga
   105361 gaatccagct ccagtggtgt tgaaggcaga aggagatcca acagggtctc ttccaatgtc
   105421 tggatgaact catttgtttt ggtaagacct ttttcatgga ttgcagtact gacacgttca
   105481 atgatacgct gactggtttc cacgcccacg tctgcctgaa tcaacagggc ttccagttca
   105541 tcccaggttt ctctggtaat ctccgacccg ccaaacacgg taataattct gccgaaggtg
   105601 gctttgcgag ttttttcgag gctctcacgc catttgttga ataaagcgct catcgcttgc
   105661 gattataaca tgtccgtttt aagcgttacg cgcgcctggc tgccagttga ccacaccccg
   105721 cggaaatatc gattcccctg cgcgccctga tggtgcaggg gatgccgttg ttttccagta
   105781 cttgcttgaa ctcttgggcc cgttcttcgg tagttgcttt tccattgtaa ctggtcgtgg
   105841 gattcagcgg gatgatgttc acgtgacaca acagcccctt gagtaaagcg gcgagctcac
   105901 gcgcatgttc gacggagtcg tttatatctc tgatcaaagc ccactcaaaa gtgagccggc
   105961 ggttggtcag cctgatatat tcgccgcagg cttccatcag cgaggaaagt gggtacttgc
   106021 ggttggctgg aatgagcacg ctgcggagtg tgtcgtttgc tgcgtgcagg ctgatggcga
   106081 ggttaacctg ccgtttttca gccgcgaacc tgcgaatacc agggacgata cccactgtgg
   106141 aaatggtgaa gcgccgcgct cccaggttca aaccgtttgg atcattcagc cggtcgatgg
   106201 cttctaaaac cgcatcgtag ttatgaaacg gttctcccat acccatgatg acaatgttgg
   106261 taatcttttc accactttgg gataactcac gacccaggaa caggacctgt tcaatcactt
   106321 caccagcgct cagattgcgc ctgaagccca tctgaccggt tgcgcagaaa acacagccca
   106381 tagcgcaacc ggattgcgac gagatgca
//
LOCUS       DAOTLW010000002        82372 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_314_length_82372_cov_11.263238, whole genome shotgun sequence.
ACCESSION   DAOTLW010000002 DAOTLW010000000
VERSION     DAOTLW010000002.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 82372)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 82372)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..82372
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_314_length_82372_cov_11.263238"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..603)
                     /locus_tag="P5516_00410"
     CDS             complement(<1..603)
                     /locus_tag="P5516_00410"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT90933.1"
                     /translation="MGEPLLKVQGLETHFKTPEGTVHAVNGVSFHIKEGETLGFVGES
                     GCGKSVTMLSCLRLIPAPPGKIIAGEAFFKGNDLLKMTPDEIRHVRGGQISMVFQDPM
                     TSLNPVMSIGKQMAEPLMLHLNLTKEQARKRSIELLGLVGIPNAADRLKDYPHQYSGG
                     MRQRVMIAMALSCNPQILIADEPTTALDVTIQAQIVDLVKH"
     gene            complement(728..1618)
                     /locus_tag="P5516_00415"
     CDS             complement(728..1618)
                     /locus_tag="P5516_00415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90934.1"
                     /translation="MKPAVSKRKQKAGLLYGLAAGLAFAIFTWGVDAFLLACAHGVFP
                     WVNFVPGLVIGLVSSALVGWLTARVERAWFSLLLWLLQALLFAHLVVWLPIKVAPRII
                     FIFNPALGDFLQYPFHHELYQNLWISFAVIALVSLIIGLIENILIEQALFSSGTFSLI
                     VPLVVCFLSFSLVGNFSDSILNKRFRESVQQVDEVIDFILAHEGQEVPQETALKMRLF
                     AFDHVKDVVSQKHGLIMSNYDEMLGQVDVLVDFNGQWVKCPVIYNQLVTCSRIFENPA
                     IRLGSTDKHMSTSVNEDPLI"
     gene            complement(1791..2702)
                     /gene="rsgA"
                     /locus_tag="P5516_00420"
     CDS             complement(1791..2702)
                     /gene="rsgA"
                     /locus_tag="P5516_00420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HRT90935.1"
                     /translation="MSAETLKGIVTRFQSGFYNVQTAGGMIVCKLRGRLKRRSFQGDV
                     ISIGDNVAITRLPDGSGVIEAIEPRARELARLDPTPRGTYRQILLANPNQIVAVFACA
                     SPEPHLRMLDRFLVIAEKQGLGAVVVANKLDLVGKRNARRIFSIYPPLGYRVIYTSAV
                     TGEGVDELREALSGKVSALTGPSGVGKSSLLNRVQPGLELAVQEVSARKDRGRHTTVV
                     RELFPLDGGGYVADLPGLRKLSLWDTEPEELDGYFPELRELVKHCQFNDCAHYEEPGC
                     AVIKAVEAGKVSAERYESYLRMRVGDE"
     gene            complement(2705..4885)
                     /locus_tag="P5516_00425"
     CDS             complement(2705..4885)
                     /locus_tag="P5516_00425"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HRT90936.1"
                     /translation="MKINHFKFPWQILVTALLVLTACSNGAQPTIEPGGELPSPIVQT
                     TQLPDVSLTVLEYLEKWNQQDYSGMYAMLAPTSRDAISEADFVKTYTDVALNMTLREI
                     EYGILTTLVNPTSAKVGYQVSFKTSLFGDIIRRMEMGLLADHGKWWIQWESGLIMPEL
                     SGGKKLALSLTSPTRGDIYDNDGNIIAAEAEAVAIGVVPDEIADDQVGNLAGILTVMT
                     GIPSNMIRDTIERGGGSYIPLGEVSAQTYNRYANYLNSFTGVAVNPYTSRYYYDGGIA
                     PQVIGYLLSISPEQYEDYRRKGYAGDEKVGAAGIEKWGEEYLKGKPAGDLYVVNADGS
                     YDTLISKTDPKPPSMIYTTLDKDLQILVQKALYGFSGAIVVLEVDTGRVLAMASSPSF
                     DPNYFNPQNYNSQWALQDLVNDHARPLWNRAAQSAYPLGSVFKLVTAAAALESGLYTP
                     DSRYECTSQYTELPGFVGNDWTFDKKKPPSGNLTLVQGIMRSCNPWFYHLGLDLYRQK
                     GATYLSDWAREFGLGEATGIGAVAEEVGQINDPTTEGAAVQMGIGQGDMLVSPLQAAN
                     MVAAIANGGTLYRPQIIEKITSVDGAVLKQFEPEVLHKVPVSQETLQALKDGMELVVR
                     DQRGTAYTRFTGLRTPIFGKTGTATTSIEDPHSWFAGFTDANNPQKPDIAVAVILENA
                     GDGSVYAAPVFRRVIEAYFTGEISRYYWWETDYYVTRTPEPDTE"
     gene            complement(4895..5563)
                     /locus_tag="P5516_00430"
     CDS             complement(4895..5563)
                     /locus_tag="P5516_00430"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HRT90937.1"
                     /translation="MSEPMIVLASNSPRRRQMLAWMEVPFTTTMAAIDETPRAGENPS
                     AYASRLAQEKARACERQAPFNALILAADTTVTDGSQIIGKPLNADDAARMLRQLRSRH
                     HMVHTAIVLALPSRGISELELCSTRVKMRAYTEEEIVNYIESGDPLDKAGAYAIQNET
                     FNPVEKISGCYASVMGLPLCHIERALRRLGFGVRKRVPYRCQKELAYACPVFKRILAG
                     ENVG"
     gene            5652..6224
                     /gene="xpt"
                     /locus_tag="P5516_00435"
     CDS             5652..6224
                     /gene="xpt"
                     /locus_tag="P5516_00435"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HRT90938.1"
                     /translation="MQALKERVAAEGKNLGDGILKVDSLINHQVDPKLMDLCGLEFAR
                     RFALTNPTRILTAEISGIAPALMTARHLNLPVVYARKSRPVTMPDTVYLTIAPSHTKG
                     NKVELIISPEYLKPGEKVLIIDDFLASGATILGLVRLTQAAGASIVGIGTLIEKTFEG
                     GREALQYLKVPIEALVQITSMEDGKIIFAP"
     gene            6239..7795
                     /gene="guaA"
                     /locus_tag="P5516_00440"
     CDS             6239..7795
                     /gene="guaA"
                     /locus_tag="P5516_00440"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HRT90939.1"
                     /translation="MKTAIAILDFGSQYTQLIARRVRELQVYCEIYPWHTQPEAIHAL
                     NPAGFILSGGPSSVYEENAPTLPLFILRSGLPVLGICYGMQLLAEALGGMVSASPQKE
                     FGLAEIEVTSSNPLLKTGRQTVWMSHGDQVKEMPPGFYRLASSASTFIAAMADPARGI
                     YGVQFHPEVHHTPRGIEILRAFVNDICGLQPEWTPENIIASSVERIRRQVGGANVLSA
                     VSGGVDSTVATTLVHRAIGNQLRTVFVNTGLLRLNEAEQVEKVLKQHLGIPLVTVDAS
                     AGYFDALRGITDPEEKRRVIGAKFIEVFEQEAARAGNPPFLVQGTIYPDVVESSSAQG
                     SQAQRIKSHHNVGGLPESMKFELIEPLRYLFKDEVRRVGEALGIPSQLVWRQPFPGPG
                     LAVRCLGEVTPERVRRLQLADAIFREELETANLLNQKPAPGCALLAQAFVVLLPVRSV
                     GVMGDRRTYQETAALRAVTSEDFMTADWAHLPYDLLGRTANRIVNEVPGINRVVYDIT
                     SKPPSTIEWE"
     gene            7851..9743
                     /locus_tag="P5516_00445"
     CDS             7851..9743
                     /locus_tag="P5516_00445"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRT90940.1"
                     /translation="MRLPSRFITFTLVIMLLAVVFSSAAGQNPGSITVKKLDTSAYPL
                     ISATFTALDPSGGFVKDLKTGELWLSENSAAVTSYTLEMEQVGVRFFVAINEGPTLDN
                     RFSGVMRIDRIKTALFNWVAAQPAPALDEFHLVVNEGALQFNPLEPSSWYAALDHYQP
                     DLKTATPGMASLSAAVDNALNNSLQSPKATALLFITPLPTEVQLAAMQEIIARAKSNN
                     LHIFIWLIGPQDYGGSDTAVQLERIARDTGGQFIIFSGAEELPAIADLLDPFEFVYHL
                     SYKTANNISGEYSLVLEIKRGELVLASEPVTYELAVSPPNPIFLSPPSTIERSWSETK
                     KKSASVLIPEKVNLKILVEFPDGMQRELVNSRLFVDGGLADERSAPPFDTFTWDLSKY
                     TESGLHNLQVIIEDAAGLTGQTIQLPVEVKVAERKITAAERLLEQLTLVNGIIMGVIL
                     LVLLIGLVLLVKTLKKRIKKRPVKKPADPVTQPVNIEGEYTLAPPQPETTIQWPVIRG
                     VGLAPARLIQKRSAAPVVDYLPEIPLGNDEVLIGSERRKADYVLVHSTVSPLHARIYK
                     DADGNYRIADSGSAAGTWVNYAPVSTRGVQLEHGDLVQFGRLAYIFELHSAAPRRVKV
                     LPYQED"
     gene            complement(9797..10693)
                     /locus_tag="P5516_00450"
     CDS             complement(9797..10693)
                     /locus_tag="P5516_00450"
                     /inference="COORDINATES: protein motif:HMM:NF015297.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HRT90941.1"
                     /translation="MPISSPVTRSRRAMMYVPGSDENKIVKSATLGLDCAILDLEDGV
                     AFNRKDEARGVIRQALERVDFGRTERLVRINPLYSGRAEQDLAAVLPGKPDAILIPKA
                     DSAAIVVAVEKIISAFEQSQGWQEGEIALALLIESASAFVNLAEICRASARVQALVFG
                     AEDFCADTGVTRTNSARELLYARSALVLHAAAFQLQAIDMVQINYRDTALLEAECRDA
                     VELGFSGKTVVHPSQVGPVQRAFTPSDSLIAYAREIVHGAELAQRGGSGVFTHQGKLV
                     DLPVVKRAEQILARARAAGIEV"
     gene            10757..11359
                     /locus_tag="P5516_00455"
     CDS             10757..11359
                     /locus_tag="P5516_00455"
                     /inference="COORDINATES: protein motif:HMM:NF023096.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3267 domain-containing protein"
                     /protein_id="HRT90942.1"
                     /translation="MNKPVKTLPAGYTLAGSVTLTRTADLLPMLIWSLVLPALLLLLV
                     VTVLCLTHQIADWNLFSLHSVGQIAAFFGGMLLVTLVMVIAHEGLHGLVFWCATGNLP
                     RFEFKFYYASASPGGWYLPRRVFMLATLVPLVTISLVCLALIPFASPFLRCLLILLAV
                     FNASGSAGDLVVALRLFRLPAETLSLDNGTSVYFFIPAPG"
     gene            11438..11525
                     /locus_tag="P5516_00460"
     tRNA            11438..11525
                     /locus_tag="P5516_00460"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11472..11474,aa:Ser,seq:tga)
     gene            11557..11649
                     /locus_tag="P5516_00465"
     tRNA            11557..11649
                     /locus_tag="P5516_00465"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:11591..11593,aa:Ser,seq:gct)
     gene            complement(11712..12098)
                     /locus_tag="P5516_00470"
     CDS             complement(11712..12098)
                     /locus_tag="P5516_00470"
                     /inference="COORDINATES: protein motif:HMM:NF020950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protease inhibitor I42 family protein"
                     /protein_id="HRT90943.1"
                     /translation="MKKRFVVVVFVVILLALAACSAPAAKLKLADSGSTLDLKNRQTF
                     TVALEGAPTTGYIWELKEIDPAVVELVGEPEYKSDSILIGSGGVFTYKFKTIAAGTSN
                     LKLVYYRPWKDNVEPLKVFMVQVNVK"
     gene            complement(12158..12814)
                     /locus_tag="P5516_00475"
     CDS             complement(12158..12814)
                     /locus_tag="P5516_00475"
                     /inference="COORDINATES: protein motif:HMM:NF017959.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HRT90944.1"
                     /translation="MLAWLIQSYQRLGLAGSLVILAGVLLSALFYRGKEGERYSITRH
                     FISELGELGVSRMAPLFNASLILAGLLFILMLIGVGFDMNTPWGYIAMSAGIVTAAAS
                     MCVGIFPMNQINPHTIAAMTYFRSGLVTVLLFSVAIILQPAARRVIPLYVLFFGVLSL
                     AAYTAFLLYAGRAAKKQAQNVLDTEKVRARPRFWWMAFLEWLVLISTIVWFLVVCSAR
                     "
     gene            12961..13734
                     /locus_tag="P5516_00480"
     CDS             12961..13734
                     /locus_tag="P5516_00480"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT90945.1"
                     /translation="MYETLSSDYDRFVNWHNRLAYEMPFIERIVNQIQTGSPRSLHIL
                     DCACGTGMHAIALAQRGQQVSGADLFPQMIEISRQNAANAGVQVRFEVAGFDQLAAQF
                     GQAQFDLLLCLGNSLPHLLSPADLADALDDFALCLRPGGMLLIQNRNFDLVMQQKERW
                     MEPQVHREGNIEWLFQRFYDFEEDGLIRFNIVTLKNENGAGWQSSVASTRLRPQLHAE
                     LQSALSLAGFESIHPYGSLAGDPFSAASSGNLVLTAVRT"
     gene            13750..14862
                     /locus_tag="P5516_00485"
     CDS             13750..14862
                     /locus_tag="P5516_00485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90946.1"
                     /translation="MKIEPVSSKCDLQAFINLPYQLYRRDPTWVPPLRSEQRAQFSPQ
                     RNPMLRHCEYQLFLAREGDEVVGRISAFIDHLALEHWGQPIGLFGSFECVNDPRVSEG
                     LLASARAWLRAKDMRRMRGPWSFASQEWGLVVEGYQPEPVIMAPYNPPYYNDLLTDFG
                     LTAVKDLLVYVIDARENYVIPERILQLTQKVQQRYRVRVRPVNMKRLEEDVLTITRLA
                     NASICDNWGFYPVTEEEARAMARDMKQIVNPAAILIAEEENGSPIGFAMSLPDINQVL
                     KGLNGRLLPFGFFKMLLLLPRLHQYRMWALGVIPQWQGRAIDTLLYRATYDALYTDKL
                     RLEINYVLEDNDRMNNAISKLGARPLRRYRIYEAGI"
     gene            15013..15207
                     /locus_tag="P5516_00490"
     CDS             15013..15207
                     /locus_tag="P5516_00490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90947.1"
                     /translation="MYLHLDEEEKKILLDLLDTCISDLRQEIAGTDNINYKDMLKQRK
                     QVLIKLFQALQSDQQTQSMT"
     gene            complement(15256..16422)
                     /locus_tag="P5516_00495"
     CDS             complement(15256..16422)
                     /locus_tag="P5516_00495"
                     /inference="COORDINATES: protein motif:HMM:NF015948.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRT90948.1"
                     /translation="MSATVFYGSPRQARLEASESLPAKLDLILDRLHLRERVKGEWVC
                     LKLHVGNNIGYSTIHPVFVRKVVSAIKEGGGEPFIVDVDWDVAGSESRGYTREVLGCP
                     VYPSAGLHERYHYDHEWQYKNMKAWKVAGMVEDASFLVNFAHVKGHPMCGFGAAFKNI
                     ALGCMVGETRSQIHDTSHFDRYWFKGRCPDRSLMQQIVDSCPFGAVVFDKKDPEELHL
                     HFESCNQCMRCQQVAPPGSLLIQPENFHTFMEAAAISTAITLSTFEKGKTTHLALATH
                     MTPMCDCFGFTSMPILQDAGIFGSDDIVALDKAVLDKTTDLPLILENIPSSLEPQPHD
                     GHPFAQLHGRFKDPYVLLHYAEKLGLGSQDYQLVDVLPLDDLSPRTKGFYISAQ"
     gene            17069..17965
                     /locus_tag="P5516_00500"
     CDS             17069..17965
                     /locus_tag="P5516_00500"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT90949.1"
                     /translation="MRYFVIKAEKVTKKYSHRPGIENITFEVEPGEIFGLLGNDHAGK
                     STLVSLLAGYFRPNSGHLMVFGLDSQRESRQIRERSGFFPGNYPAPSRHTVAELLRSQ
                     GHQPGGMLWTDIQKLTDQLDLDLAQPAATLSSHDRQVFDLLQAFIPRPELLVLDEPTY
                     ELKPAIQDVFYRLISEARQDGRTVFLTSRSLAEMERICDRVAIMHRGKLVAVERGIQL
                     RSRAIRVVEMRFADSISKDGFADIPNIRNLTIEGNKLRCVLQGEPDRLIKTACQHHIT
                     DLLSTQPSLEQVLADTYGIKTI"
     gene            18095..18874
                     /gene="udp"
                     /locus_tag="P5516_00505"
     CDS             18095..18874
                     /gene="udp"
                     /locus_tag="P5516_00505"
                     /EC_number="2.4.2.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HRT90950.1"
                     /translation="MRNEEGLAFHLHIQQGDIGRYVLLPGDPARCEKIARYFKDAHFV
                     AQNREYVTWTGSLLGEKVSVISTGIGCPSAAIAVEEAIEAGADTLIRVGSSGSMQPHI
                     HTGDVVVVSAAIRDEGTTPYYLPIEFPAVADIEVVNALREAAERLGYKSHTGIAQSKD
                     SFYGEVERARMPMPARLAERWNAFITGGAACSEMESSALFILSSIYRKRAGGVMLVIN
                     ESDLAEVDEDNPAKHMAEFDADRVIKVAVEALKILIQSDRR"
     gene            18887..20116
                     /locus_tag="P5516_00510"
     CDS             18887..20116
                     /locus_tag="P5516_00510"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT90951.1"
                     /translation="MKPTTLHSRITSLSRELALFITASFAVGMAASLVDSTLNNFLND
                     TFSLNGFQRSFLEFPREFPGVIVLFVSAALWFLGSRRLGAFAMLLAALGIFLIGFSSS
                     SYGLMVIFLFIYSNGNHLFMPVATTIGMELAADGRTGRRLGQLNAIRNLATIAGSFLV
                     FIGFKFLGFTYQHSFTIAALVFTAAAVLMFLMKPEKSAPPKTFLKLRKEYTLFYALAV
                     LAGSRKQIFITFAPWVIVTIFNKPTQTIATLVTIGGVIGILFQPFLGKAIDRYGERLI
                     LSAEALVLIFVCLGYGFSRSVFAENVAFLIVCVCYLLDQMLFSVSMARSTYIKKIALD
                     PSDIQPALSASISIDHVFSISVALLGGVIWNNFGFQYVFLLGMLIAMVNFMVARRIVI
                     PKPSITEKPIPQPIERD"
     gene            20221..21279
                     /locus_tag="P5516_00515"
     CDS             20221..21279
                     /locus_tag="P5516_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013226.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRT90952.1"
                     /translation="MKVNFSMWKKALSTLVKMDSKEEWDELDVVSKWLIATRSAVTIV
                     TLYSCAIGGLLAIRHGYFNDNWGMSILAWVIVTLGLFIAHGTNNFLNDYTDFARGVDT
                     ESYFRTQYSVHPLIQKFWSKEQQFRWFAISGTLAMLAGLFALFYTHFSLDIIILFAVG
                     ALVLLFYTWPLKHIALGELSIFLIWGPIIVAAVFLVLAKGSTEHVWPVALAAVPWGLS
                     VVSINLGKHIDKAPEDKKKMVGTLPVVIGEKAARITNIILLALIYLFILYLVFISRYF
                     SPILLIVFFAGQRMLYAIGVHTKPRPAEPPQDWPYWPTWFAGFTFYHNRLFSNLLLIA
                     ILIDTLLRLFLPGFWPAQ"
     gene            21311..22705
                     /locus_tag="P5516_00520"
     CDS             21311..22705
                     /locus_tag="P5516_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HRT90953.1"
                     /translation="MDIVFLSRWQFALTTIYHFFFVPLTLGLGWFVAILETMYARSGN
                     LLYKRLTKFWGKLFLINYAMGVVTGIVMEFQFGMNWSEYSRFVGDVFGAPLAVEGLLA
                     FFLESTFLGVWIFGWERLSKKLHAAVMWLVAIGGNLSAFWILAANGFMQHPVGYAIVN
                     GRAQMTDFGALLGNPRALYLFLHTFFAGLVTASFFMLGFSIYHLVKKQEVEAFRKSFV
                     LSALIGLLSTVAVGGAGHIQGVYLREVQPMAAAASEAHYETANPADFHLIAGIDQDGR
                     KTTWSVTIPRALSFLYFFKWEGEVEGLNQVQARYEQLYGPGNYIPNIALTFWSFRVMV
                     GVGLLMGLLSLGALVIAWKKYPEKWIERSGWLVWIILLPYLANTTGWVLTETGRQPWV
                     VHGLMQTKDAVSPNLAPATVLISLIGFVLIYAVLMVIDLYLLVKFAKKGLGAGDLDSV
                     PITGEVEAKKKGGK"
     gene            22702..23721
                     /gene="cydB"
                     /locus_tag="P5516_00525"
     CDS             22702..23721
                     /gene="cydB"
                     /locus_tag="P5516_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HRT90954.1"
                     /translation="MMNLNILWFVLVAVLYAGFFFLEGFDFGVGMLLPSLGRKDVERR
                     AIINTIGPHWDGNEVWLIVAGGATFAAFPQWYATLFSGFYLGFFLLLFALIVRGVGFE
                     FRSKLSAARWRYGWDWAIAVSSFVAAFLLGVVFTNLVRGVPINAAMQYTGGFFTLLNP
                     ISLLGGLTVVMLFLFHGANFLTLKLSDGLRERARNATKTYFTAALLFALGLFAALLFT
                     PSAFSTKGVFSAVLPVLAAASLVVSRVMMGKKKEGWAFICSSLAVVLTVAALFTALFP
                     NVMVSTTNPANNLTIYNASSSDYTLKVLSIIAAVLVPVVLAYQTWTYVIFRKRVKADT
                     KSLTY"
     gene            complement(23755..24459)
                     /locus_tag="P5516_00530"
     CDS             complement(23755..24459)
                     /locus_tag="P5516_00530"
                     /inference="COORDINATES: protein motif:HMM:TIGR00035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HRT90955.1"
                     /translation="MQTIGLIGGMSWESTLEYYRILNLETKKRLGGFHSARCVLYSVD
                     FAEIEEWQRQGNWQEAARCLLAAGRALQAAGADFILLCTNTMHKLAPSLEAELGIPLL
                     HIADATADAVKAAGITQVGLLGTRFTMEQDFYRDRLAKKHGLNVLVPGEAERQIIHEV
                     IYQELCMGVVKESSREKFSRVISNLADVGAQGVILGCTEIENLVRPQDSVLPIFATTK
                     IHAEAAVNLAIKNRGN"
     gene            24569..25294
                     /locus_tag="P5516_00535"
     CDS             24569..25294
                     /locus_tag="P5516_00535"
                     /inference="COORDINATES: protein motif:HMM:NF019456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HRT90956.1"
                     /translation="MRTTFEPLQVTSLKEACITRLEELILSGELKAGERLPAERDFAA
                     RLGVSRPVLHEALVDLAAKGLVTIHPRRGVLVNDFRVSGSCAILSSLLTYHNGSLDVD
                     FTESLLAMRVLVETETARLAALYVTPQQERSLEEIIANEAACDRADPLTRTSLDFDFH
                     LAVAIASGNLVYPLILNSFKKVYTRFTGVFFSHSAGSAVMDEVLAFHRRLVSAIREHQ
                     PEVAAAVMRELLLHGEQILKEIL"
     gene            25317..>25555
                     /locus_tag="P5516_00540"
     CDS             25317..>25555
                     /locus_tag="P5516_00540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90957.1"
                     /translation="MSTQTTDKPRFDIKAPRNLSERISWLRDYYFEGVNRKWNNEYIS
                     YTTGTDWDFQYNELSFYIVPETYFYIPTFRSSFKQL"
     assembly_gap    25556..25655
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <25656..>27037
                     /locus_tag="P5516_00545"
     CDS             <25656..>27037
                     /locus_tag="P5516_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase family protein"
                     /protein_id="HRT90958.1"
                     /translation="ALPPAQQKGKKGAQLRAMLTASTLARDLATEYQALLVSLAHAEQ
                     DPMRKTELEQMAHNLSRVPWEPAETFWQAVQSLWMTHMLVMADECYPGPGVSFGRIDQ
                     YLNPLWERSLAEGMQREFGKEILKCFWVHCNTAYDAMIQTGGNQGITAGYGQLLTLSG
                     AGKGGVDMTNDLTFAMLEVIDEMTPILEPKPNVRLHRGTPDDLLTKVVDMISSSQGAP
                     FLLNFDERSMAGMMLEARKAGVEHLIHAENVHEYAPVGCLENTMVGNDRSGTVDDNLN
                     LLKAVELTLTGGYDLNPVVDALSGKAEKPRRDGENSGDPASFTTWEQFWEAYALQTRY
                     IIRKSVETYERSEEIRAKYFQTPYVSCLVKGCAESGKDINEGGAELGFVTIEGVTFAT
                     TVDSLLAVKYLVFDHAICTMNQLVQALRDNWVGHEKLQAIALYKAPKYGRDEDDADAM
                     ARQVMALWTE"
     assembly_gap    27038..27137
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <27138..27338
                     /locus_tag="P5516_00550"
     CDS             <27138..27338
                     /locus_tag="P5516_00550"
                     /inference="COORDINATES: protein motif:HMM:NF013399.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine radical domain-containing protein"
                     /protein_id="HRT90959.1"
                     /translation="YLRGYIENGGSALQINMLNADMLRDAQLHPEDYRHLLVRVTGYN
                     AYFTAIGKELQDEIIARESHQM"
     gene            27338..28309
                     /locus_tag="P5516_00555"
     CDS             27338..28309
                     /locus_tag="P5516_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR02494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0043687 - post-translational protein
                     modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycyl-radical enzyme activating protein"
                     /protein_id="HRT90960.1"
                     /translation="MSSENVSTRGTILHLQRLSTEDGPGIRTTVFFKGCTLRCEWCHN
                     PESLSRHRQVQWLANRCIGCASCVAACEVHSLTLTVEGIVRDRGLCVTCGRCVEACPS
                     GAMEMLGKEVSAAELTAELLKDRAYFEKSGGGVTLSGGEPTLQPAFCAALMDALHAEG
                     VHVALDTCGLSAPGVFSELAAKADLILFDLKLMNPDEHKRWTGADNSRILRNLEIARD
                     FVSRSGGAKRLWIRTPLIPGATTAPDNLAGISAYLGASFDGSVERWELCAFNNLCRDK
                     YERLGMRWTFAETELLTAQQLTQMGEWARSGGFDPSRTFVTGAAKAS"
     gene            28350..29207
                     /locus_tag="P5516_00560"
     CDS             28350..29207
                     /locus_tag="P5516_00560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90961.1"
                     /translation="MNSSLYPRNSFSEQEIKDLSAAMKVGILGTVNPQGLPHLTLITT
                     LMASSPQVVVWGQFMEGSSKKHVLTNPKTGFMVMGLDKNFWRGFADYSHSETSGKDYA
                     YYNNTPLFRYNAYFGVHRVHYMDLIAHTGKHPLPMQRIIPAAVMTMVAKTLSAKPQKT
                     PVLNPWTQAFFNKIDNLKFLGYVNAEGYPVIIPLIQAQALDREHLIYSLGAFGDELAQ
                     IPPYTPVAVFGMALSMEDVLVRGTYQGIKRVAGIRCGLVQLDWVYNSMPPTPRQIYPE
                     VVIEAVSEF"
     gene            29376..29984
                     /locus_tag="P5516_00565"
     CDS             29376..29984
                     /locus_tag="P5516_00565"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRT90962.1"
                     /translation="MQRTIVFDFGGVLIDWSPYHLYRKLLPSDDAVREFLEEIKFSEF
                     NKKLDAGYPFHKMKEELLEKYPHRYQLVRAFFERWMECTGEAVRPAVEIMREIKRAGY
                     PVYGLSNWSTETFPLLQHRYQFLPELDGYLLSGMAGVAKPDEEIFRIFLARFERNAED
                     CVFIDDTQANVDAARRLGFNAILFTSAGQLRVALVKIGVLKA"
     gene            complement(30101..30511)
                     /locus_tag="P5516_00570"
     CDS             complement(30101..30511)
                     /locus_tag="P5516_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90963.1"
                     /translation="MKKIYLTHCSKEKDPEFALNGEKVTPDRLYTSEGIQQFIKYCNE
                     HSHYWAILSDRYGVVFRDDHIKWYNQPPDTVTEEDYERLLQSFITRLSFFDEVYFYHR
                     PGETHPLFVRIIEAARAQGMKVSDFTQASMQKGE"
     gene            complement(30508..31704)
                     /locus_tag="P5516_00575"
     CDS             complement(30508..31704)
                     /locus_tag="P5516_00575"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT90964.1"
                     /translation="MSPQSKFTRLMMFALLYFTQGTILGYFASLNALYMLDNGLDMAA
                     VGIFSTIALIPFVIKILFGILSDRYNLLGLGHRKPYIFIGLLVQFACLIVVANINPGR
                     SYWLYVGLAFLLQLGMAFYDTCTDGLALDITPETEKGTLQGFMVGGRSVGVIVAASVA
                     GLIAQHLSWQGVFYFLAILTLVPIPFLFFIKEIQKTAEMRFQWSAFRAFKNVQVLAVA
                     AVGLVIFLVIVGANQLVNPSLDAKLGIDLSTAGLLTTVWGIGCVVGALAGGRVMDKLG
                     NQKALWLSILLVVPAMILLAMVANRPMMWAVIVFFGVSYGLSLAIYFALAMKYTQPAI
                     AASMFSILMAVTNVGQGIGLGLGGGLANAAGYPLTFLVFGALMLLVILFFPVLFGVKR
                     KGQGNQ"
     gene            31783..32667
                     /locus_tag="P5516_00580"
     CDS             31783..32667
                     /locus_tag="P5516_00580"
                     /inference="COORDINATES: protein motif:HMM:NF012334.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HRT90965.1"
                     /translation="MTGDFSSKNVLVTGASSGIGYQAALDLAGKGAYIIGTGRDVTRC
                     EAARTRILQVHPEAQIEYLVADLASQTQIHDLARQAQLLLIEHGNRLDVLVNNAGLYS
                     SKRVLTEDGIELTFAVNHLAPFLLTHLLLPQLLNTPQGRVLGVSSQSHYHAFLSPLKA
                     YRPWLYLSLPVYATSNLCRVLFSAEFNRRTPTPHLHAWSVDPGLVNTEIGQKDKGFLS
                     NLVWRFRKTHGTSAEIPSQTILHLAFSAFHHIAADLYWKDSRPKEPSRLSRDAELAKR
                     LWEQSCRLCGIDDYFNPG"
     gene            complement(32654..33433)
                     /gene="ygiD"
                     /locus_tag="P5516_00585"
     CDS             complement(32654..33433)
                     /gene="ygiD"
                     /locus_tag="P5516_00585"
                     /EC_number="1.13.11.29"
                     /inference="COORDINATES: protein motif:HMM:NF014899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4,5-DOPA dioxygenase extradiol"
                     /protein_id="HRT90966.1"
                     /translation="MIPVTRLPVLFVGHGSPMNAIEQNEFSLTWEELGRVMPRPRAVL
                     CISAHWETWDTRVTAMANPRTIHDFYGFPRELNEMQYPAPGSDELVELVKQKIKSTTV
                     VEDQEWGLDHGSWSVLCRMYPKAEIPVVQLSLNRQLSVQSHYLLGRELRPLCDDGILI
                     LGSGNMVHNLRMVTWEADAGYDWAVSYDADLKNWIEKGEHEQVVHYEEHGKPAELAVN
                     SAEHYLPMLYVLGAGYADEPVSFFNEKVIMGSISMRCFSLG"
     gene            complement(33430..34473)
                     /locus_tag="P5516_00590"
     CDS             complement(33430..34473)
                     /locus_tag="P5516_00590"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT90967.1"
                     /translation="MDRNKVSELTGLLTRALYHRQALLEQPPRSAIRLFNGFYEGFPP
                     LVVDLYARTLVVFTHKLDAADSLALAQMVQQHLLAQLDWIGCVLLKQRSSTHPDAKKG
                     VVLYGAQPDTVVWENGVQYALALRLNQDAGFYLDTRGLRAWLKENASNQSVLNTFAYT
                     GSLGVAALAGGASAVTQIDRSARFLEVARASLRLNALDETKMKTSAVDFFVAAGQMRR
                     KRETYDIVIIDPPFFSLTPRGRVDQVKETTRLINKARPLVTDGGYLVVVNNALFLSGR
                     EFIGELEALTENGLLTIEHIIPVGEDVTGYAETVVNQPPVDSAPFNHPTKIVVIKVKH
                     KARAAEKANKEVK"
     gene            34539..35324
                     /locus_tag="P5516_00595"
     CDS             34539..35324
                     /locus_tag="P5516_00595"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRT90968.1"
                     /translation="MNQTLRIIHHRRSTRKYAATPITSEEKDAILQAALRAPTAGNMM
                     LYTIIEVEDQALKDRLSVSCDNQPFIASASYVLLFLADYQRWVEAFNWSDAPRLAMEQ
                     GLQPRLPQEGDLLLACCDALIAAQTAVIAAESLGIGSCYIGDIMENYEVHREMFRLPR
                     FTFPVTLLCFGRPYAEREHPGLTRRFGRNIIVHRDHYTPVDADAFPAMLSRAGQQSPD
                     EVDVEAARKMAQGIYTRKFVSAFSVEMTRSVKRWMEIWTGAED"
     gene            35328..35666
                     /locus_tag="P5516_00600"
     CDS             35328..35666
                     /locus_tag="P5516_00600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT90969.1"
                     /translation="MGELDVPTSRASGEDWLFYDAPLVLHFSGSAYSDPADCAVVASL
                     AMLAGESLGLGSCMLGFPGYIFRYSKPLREKYVLPPKIQPGLTVVFGYPRFTPRNAVR
                     RRFREVRECA"
     gene            35703..35972
                     /locus_tag="P5516_00605"
     CDS             35703..35972
                     /locus_tag="P5516_00605"
                     /inference="COORDINATES: protein motif:HMM:NF016262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF493 domain-containing protein"
                     /protein_id="HRT90970.1"
                     /translation="MVDDKTPEVEYPVEFPLKVIGVDENDFEPFVVEIVRRHVPDLLE
                     ENIVSRLSNNNHYRSVSFHFIAQSREQVDALYAELSSHKRVLMIL"
     gene            complement(35969..36880)
                     /gene="gnd"
                     /locus_tag="P5516_00610"
     CDS             complement(35969..36880)
                     /gene="gnd"
                     /locus_tag="P5516_00610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HRT90971.1"
                     /translation="MQIGMIGLGKMGMNMAQRLIKGGHSVIGNATKETTIREAEALGV
                     KGARSLQELTANLAQPRVVWLMVPAGAVTEEVLGSLTPLLEMGDIVVDGGNSNYKDTT
                     RRGQVLMEKGLRFVDVGTSGGIWGLTEGYSLMIGGDPATVEYLRPIFETLAPAPDQGW
                     GRVGPSGAGHFTKMVHNGIEYGMMQAFAEGFEILKEKREFELDLHRVSQIWQRGSVIR
                     SWLLELIENVMAEDSSLEDVEAYVADSGEGRWTVFEAIDLNVAAPVITASLQRRIRSR
                     QDGPYSDKLVAAMRHQFGGHEIKKKEG"
     gene            complement(36987..37577)
                     /gene="plsY"
                     /locus_tag="P5516_00615"
     CDS             complement(36987..37577)
                     /gene="plsY"
                     /locus_tag="P5516_00615"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HRT90972.1"
                     /translation="MKLLQITGLFLLGYALGNFSPAYLIARKVGRFDIREKGSGNAGA
                     TNVIRTLGWRYGLLVFLLDALKGATAAGAGYWLGGETGLAAAAIGVVLGHDFPVVLNF
                     RGGKGIAATTGILLSLLPLPTLAGILVFVLVVLGTRMVSLGSLVFVFGMAVYVIVTQQ
                     PLMWVVVVVVAALFALLRHHENIRRILAGVENRISL"
     gene            37718..39241
                     /gene="zwf"
                     /locus_tag="P5516_00620"
     CDS             37718..39241
                     /gene="zwf"
                     /locus_tag="P5516_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HRT90973.1"
                     /translation="MTDSTSIVIFGASGDLSHRKLIPALYHLYLKGRLPKSFRVFGIA
                     GRPWTDEDLRRVSRDGLNQFSRLPVDENSWDTFSQTLSYLSGDISRAETYHQINERLS
                     LLEGGEANRMYYLATPPEYFLEIINNLAANGLLQEDDAWRRVVIEKPFGEDLESARSL
                     NQSIHAVMKEKQVYRIDHYLGKETVQNILVARFANAIFEPVWNRNFIDNVQITVAETV
                     GVGTRARYYDHAGVLRDMFQNHLIQLLTLVAMEPTSTYNADALRDEKLKVIRAIRPMN
                     RDEIMKNSVIGQYRGYQQEPNVAPGSRTPTYAAVRFYIDNWRWQGVPFYLRSGKKMTE
                     KVSEILIQFKKPPHMLFPMSGSDLEGRSNLLSMCLQPDEGIHLRFETKVPDTVAETRS
                     VNMNYHYSDVYDQSAIPEAYERLLLDVITGDASLFTRADQSELSWALFDPIIAGWEDP
                     AAPQPEEYEPGSWGPDGSERMLRAEGRQWLQTCGVHCEENHKTCDEKRVDPSRQQPW"
     gene            39316..40338
                     /locus_tag="P5516_00625"
     CDS             39316..40338
                     /locus_tag="P5516_00625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRT90974.1"
                     /translation="MKPALPCNNPPRCWSIPLLIFTAALLLFLSACQPAGPAASEPIN
                     ALSQETPPAQTPLLATATQHDTLSLSPTETSPPVPTQTTIVRTPGFWKELPILPEELS
                     VRAREIYQQGLAMGNDPRVYTKIGDCNSMSPDFLAGFGGQYDLGEDFAYLQPAIDYFN
                     KSYRLPNQATNPGTTTSRLLVSLWNNDECLPNEPMLECQYRVDNPSIAIIMLGTIDAK
                     THQRQPEVYEQNLRVIIEKTISLGILPVLATKADNIEGDNSINETIARLAIEYELPLW
                     NFWKAAQPLVNHGLQTDGEHLNFWSAPPSTDFSLPIALDYGKEVKNITALQLLNFLME
                     ELPAPQ"
     gene            complement(40372..41019)
                     /gene="folE"
                     /locus_tag="P5516_00630"
     CDS             complement(40372..41019)
                     /gene="folE"
                     /locus_tag="P5516_00630"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF006826.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HRT90975.1"
                     /translation="MTDTYVDDFSVDELLQLNQRKISAEELKKYEKYMTEIFSALGMD
                     VNTPSTHETPRRFLEALIESTSGYEGDPKLIKSFPRECRGEPDCRLSQVIEGPIHFHS
                     LCEHHVFPFYGEAYVGYVADERILGISKLTRLVRLYTKRFAVQERIGYQIADTLEALM
                     RPHGVAVFMRAKHMCVEMRGVREISPATRTTVFRGVYADDPALRAEFLNVCGIMK"
     gene            41192..41599
                     /gene="arfB"
                     /locus_tag="P5516_00635"
     CDS             41192..41599
                     /gene="arfB"
                     /locus_tag="P5516_00635"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF006718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HRT90976.1"
                     /translation="MKQIPENEISIRYVHSSGPGGQHVNKVATTAQLRFDIKNSLVLS
                     EHVKERLFRLAGRRVTGDGVLVIEAGRFRERERNRQEAINRLFRLVEQAQQTPQNRIP
                     TRPRASDITKRLEAKKRRALIKFLRHFRPEREE"
     gene            41709..42362
                     /locus_tag="P5516_00640"
     CDS             41709..42362
                     /locus_tag="P5516_00640"
                     /inference="COORDINATES: protein motif:HMM:NF025597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4339 domain-containing protein"
                     /protein_id="HRT90977.1"
                     /translation="MWYYTLNNQQVGPVEEAEIKKLVEAGTITHGTLVWTNGMANWLP
                     IGQTPLAGLLGSVPPPVAPPPMAVVYEDPEVASMKKLFMWFWISLIGILLSGIGLIPA
                     GVLFFVIFYKAWKQIEHEGIRANADQATSRCFIPGWNYYWLFVAFRGLAKEQGDKLDS
                     LNVSLEKIDPKLPTWMLICLLGSAVTFGVSLIAFLVLWIMYTNKVKNNYLAIHQASK"
     gene            complement(42429..43874)
                     /locus_tag="P5516_00645"
     CDS             complement(42429..43874)
                     /locus_tag="P5516_00645"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRT90978.1"
                     /translation="MTLIDFNSILPVILLVVWALVMLLLDVWFGKRVPLLTPVMAVAG
                     LVLSLAASLYQIGQQASAFNGMIRVDGFSIFLNNLFALSGIVAIGLGYDYLKRVGISE
                     GEYYVLLLFSISGMMLMGSAGDLIIVFLSLELLSIPLYVLAGIDHKRDASREASLKYF
                     LLGTMASAFVLFGIAFVYGAAGTTSLYGITEAVSQGKVQQFLLICGSALLLAGFGFKV
                     AAVPFHSWTPDVYHGSPSSVTAFMSVGAKAAGFAALIRVFSLNFTAIAIELTPLFWVL
                     SALTMIVGNVVAVAQTNLKRMLAYSSIAHAGYILMAFVGFGDAAVRQNSIASALFYLA
                     AYALTSLGAWSVLVAVEQQDGSGNEISDLAGLGKTAPSLAAAMVVFMLSFTGIPLTLG
                     FWGKFYLFRTAIEGGFTYLAVAGLLTSLVSAFYYLRVVVKMYFQPGEALAHKGRLTSL
                     IATILAILVVGLAFVPGYLFNLAAAGLIAGA"
     gene            complement(43889..45394)
                     /locus_tag="P5516_00650"
     CDS             complement(43889..45394)
                     /locus_tag="P5516_00650"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRT90979.1"
                     /translation="MNSAINPLLLVTFFPLVGVLLILLLGSTGKNTARWIALLTSLVT
                     FALSLWLLMQFDSTKGGIQMEINLPWLTLGGNTVGFHLGVDGISILMVLLTALLTPIA
                     ILSTWKAVEERVSGFMSFFLLLETGMMGVFLSLDMVLFYIFWEFTLVPMYFLIGLWGS
                     QRRVYAAVKFVLYTMAGSLLMLAGILWVGLTYGGFDLTKLTDSAIPFTAQLWLFGAFG
                     LAFAIKVPMWPLHSWLPDAHTEAPTAGSVILAGVLLKMGAYGFLRFNLPLFPQATLTL
                     APWMAGLAVIGILYGAIVAFRQKDVKRLVAYSSISHLGFVVLGTFALTAQGVQGAILQ
                     MVNHGLSTGALFLIVGMLYERRHTRLMADFGGLWRVTPLLGSLTLVTVLSSVGLPGLN
                     GFVGEFTILMGAFHSTILASPWFAVVATLGVILAAVYLLHMFQQVFLGPVRHPENEKL
                     ADLSPREVILLAPLLLLAFWIGLYPAPFFRLMSPAVEQLLALIQTVVQTAH"
     gene            complement(45405..47360)
                     /gene="nuoL"
                     /locus_tag="P5516_00655"
     CDS             complement(45405..47360)
                     /gene="nuoL"
                     /locus_tag="P5516_00655"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRT90980.1"
                     /translation="MESGQIFNLVPWVVFLPVIGLVVNLLLGRRLGEVFSGVVASLAS
                     GLTFVVAVLLAAALASAPNARVVSPWEWISIGELTVRWAFRVDTLSVVMMLAVSLVGT
                     LIHIYSIGYMHSDVRHNGDPGRYTRFFVFMNLFIASMMLLVSADNFLMMFVGWEGVGL
                     CSYLLIGFWYEKGKNGIGNAEAAKKAFITNRIGDFGLLIALFLIFGYFGTLQFDEVFR
                     QAASVAAAHPGAMLLITLAMLLGVSGKSAQIPLFVWLPDAMAGPTPVSALIHAATMVT
                     AGVYLVARSTAFYSVVPVAQTVVMWVGALTALLAATIALGQYDIKKVLAYSTISQLGF
                     MVAAVGMGAMVAGMFHLVTHAFFKGLLFLSAGSVIQGMERGMHGGDDSAEFDPQDMRN
                     MGGLRKKMPLTFWVYLIGGLALSGIAPLAGFFSKDEILGAAYTGHNAVFVLLVLTAFL
                     TAFYMGRQLVMVFFGKPLSAAAEHASESPRVMTIPLVILALLSLLGGALNLPGVHTLE
                     HWLAHTLGEVKPAEFIWLVAGLSFLLALLGLGLAWLIYTRREQKSGADDPLKKPLGAL
                     YTGMERKWWVDEFYQALILNPYKAFAQFIAEPVDLGIIDRVGGGLAAGTRALAEGLRQ
                     LENGYVRSYALWMVVGLLAIMMFLFLR"
     gene            complement(47387..47689)
                     /gene="nuoK"
                     /locus_tag="P5516_00660"
     CDS             complement(47387..47689)
                     /gene="nuoK"
                     /locus_tag="P5516_00660"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRT90981.1"
                     /translation="MFPVIYPIILAVILFSLGVAGVLLRKNAIVILMSLELMFNAANL
                     VFITFANYHQNLDGQVFIIFIMVTAAAEVAVGLALIVNIFRAKKTISIDEMHSLKG"
     gene            complement(47689..48207)
                     /locus_tag="P5516_00665"
     CDS             complement(47689..48207)
                     /locus_tag="P5516_00665"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRT90982.1"
                     /translation="MTPALLIFILLGIIAVASALAMLLSRNTVYSALFLILNFLAVAV
                     IYILMGASFVALSQIAVYAGAIMVLVLFVIMLLGTQQLDPRETLKWQRPLAIVLTLAL
                     IIQGAYALITRYGFQYATLAASPEFVDPKNLGMVLFQKHSLPFEVTSVILLVAAIGAV
                     VLTHKQKKDGGA"
     gene            complement(48218..48709)
                     /gene="nuoI"
                     /locus_tag="P5516_00670"
     CDS             complement(48218..48709)
                     /gene="nuoI"
                     /locus_tag="P5516_00670"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HRT90983.1"
                     /translation="MLRGLITTFKSLFEKPVTIQYPEQRRPVFERYRGRHELKRYDNG
                     LEKCIGCALCAAACPSDAIFVEAAENTDDERYSPGERYASTYEINMLRCIYCGFCEDA
                     CPTEAIVLEKIYEMSYSDRHSAIFTKDMLLVKVPDGGQPTPQKTEPGLYTRSVPEMKD
                     PTD"
     gene            complement(48713..49717)
                     /gene="nuoH"
                     /locus_tag="P5516_00675"
     CDS             complement(48713..49717)
                     /gene="nuoH"
                     /locus_tag="P5516_00675"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRT90984.1"
                     /translation="MTFAMFLEWFLKALILVLGLTVGFAYVTLFERRLLARMQVRIGP
                     NRVGPAGLLQPVADAIKLIFKEELIPAKADKILFVLAPVLTVIPALIITGVIPLGPPI
                     RLFGREIALQVANLNIGILYLLTVTAISIYGIALAGWSSNNKYSTMGALRATASMISY
                     ELALGLAYVGPVLLAGSMNINDIVTAQKPLWFVVMQPLGAIIYLIGANAEINRTPFDM
                     PEAEHELTAGFLTEYSGMKFALFYMAEYIKMIAVSMIGASLFLGGYWGPGVSTYPWLG
                     PIYLAIKVILLLFWFIWVRATWPRFRYDRMMAFGWKILFPLALLNVLVTALVIVLIGG
                     "
     gene            complement(49730..52207)
                     /gene="nuoG"
                     /locus_tag="P5516_00680"
     CDS             complement(49730..52207)
                     /gene="nuoG"
                     /locus_tag="P5516_00680"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HRT90985.1"
                     /translation="MEKQVTLKIDGRSVTVPEGTLIVDAAKQLGIVIPVFCYHPKLKP
                     AGMCRVCLVDVGRPQLDRASGAPVLNEDGTPKIVFGPKLETACTTPVSEGMVVWGATE
                     KVMEARKEVLEFLLTSHPLDCPVCDKGGECPLQNLTMKFGPAESRFEFDEKSHNQKRV
                     PLGELIILDRERCIHCGRCVRFQEQIVDDPVIGFYNRGRALEIRTYSDPGFDSIFSGN
                     TTDICPVGALTTADFRFGARPWEMKDKASICTQCPVGCNITYNVRREARKDGRTVIKR
                     VMPRQNEAVNEIWICDKGRFTYHYYESPERLTAPLIRRGDELVQTSWDEAIRYAAQQV
                     KAAGSRLAMVTSGRLSNEDLFALKQLADVTGGKAILHGTISGGEAVSLAGMSPGSDLG
                     TLAQGSTILVFACDLHEEAPIWWLRVKQAAERGATLIVVNPRTTRLDKFATHRLSYAY
                     GNELDMLQTLQADTGDIGQAVKEAENLVFIFGSEGMGLSASAAVAEQLALWLERSGHR
                     GKPSSGLIAVWQKGNEQGAWEIGYTTDERLAETISAAMGVYICAADPASEDPELKIAL
                     QRAGFMIVQDLFLTETAKLADVVLPAQAVMEREGTAVSGERRLQYYSPAVPAPAGTRP
                     DYAIIRAIAEEAGIQLKGENTADLFEQLTDSVEVFKGITLKEVRAVREQKPALGRSHM
                     YYGGTSYENREGLGVTLPLRAGALRSSAEHYQPQALTLESGSLLAVPITRLYDRGGTV
                     ATSGMLQQRIVRSLFSLHPADAELFQVKAGERISLRLDSREIEGEVDINIGQPQGVVL
                     VTRSMGALLSAPVVVKLTVPQRSAVNS"
     gene            complement(52212..53462)
                     /gene="nuoF"
                     /locus_tag="P5516_00685"
     CDS             complement(52212..53462)
                     /gene="nuoF"
                     /locus_tag="P5516_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HRT90986.1"
                     /translation="MSELWLLRHRQIPGLNQLSTYLQHGGFESWKKAVTKLTPEDLVN
                     EVKNSGLRGRGGAGFPTGAKWSFLPNTIWPHYVVANADESEPGTFKDREILEFNPYQF
                     LEGLMLASYAVRAHTAYVYLRGEFWHIAHKLDERIAELTSSGYLGDNLFGKDYSLRIH
                     THLGAGAYICGEETALLESMEGRLGQPRLRPPFPAVSGLFGYPTVINNVETLTNLPLI
                     IGKGAAYFRAQGTEKSPGTKIFSLSGRVKKPGNYELPLGTTFRELIYQHGEGIINDAQ
                     IKSIMPAGASSSLIPATEVALDTPMDYESVGALGCALGSASVIILDETVNASTVVSDT
                     IHFFKHESCGKCTPCREGTFWMARVIDRIEAGKAKEEDIDLLASIASQMQNKCLCALG
                     EFSTVSVISAIKNYRADFAARLEK"
     gene            complement(53455..54009)
                     /locus_tag="P5516_00690"
     CDS             complement(53455..54009)
                     /locus_tag="P5516_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013426.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HRT90987.1"
                     /translation="MNQLLKKFPQEIKKILAKYPPDQKRASVMPLLHLAQQDRGYISR
                     ETMAEIAEIAEVEVTEVASVVGFYTLFHTEPGGKIRIQVCTDIACALRGSREYLDGLC
                     AELDIKPGGTTPDGLVTVEEVKCLAACDRAPMFQSQMGDELEYHEHQTVARTMDWLRS
                     MMASLRLPESDEPLSPEPEGGDHE"
     gene            complement(54006..55235)
                     /gene="nuoD"
                     /locus_tag="P5516_00695"
     CDS             complement(54006..55235)
                     /gene="nuoD"
                     /locus_tag="P5516_00695"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase (quinone) subunit D"
                     /protein_id="HRT90988.1"
                     /translation="MREIQEFTLEELKHHLSEKAFSGQTVVLNMGPQHPSTHGVLRLI
                     LELDGEIIVNCIPDVGYLHTGIEKTMESKTYQKAEVLVDRMDYMYAMGNTLVYSLAVE
                     KLIGLQPPPRAEAIRVILTELSRIQSHLLWLGTSALDLAAQSTFLYTMRERELILDIF
                     ELVSGARMMTTFIRPGGLWRDVPVQFEKMVRDFTKVMPKRMAEYESMLTRNPLFLDRT
                     KGIGKIDTRTCLAYDVTGPTLRASGLAHDVRKSNPYSGYDLYDFDIPTQSEGDVYARY
                     LVRMEEMRQSVRIIEQAMDKLPYGPVRDPNYKYVPPPRSEIGVSMEALIHHFKLWTEG
                     FIPPKGAVYTAVESPRGEMGVFMESNGTPKPYRVHYRTPSGPLLHALPVIAKGHFVAD
                     MIGIIGSIDIVLGDCDR"
     gene            complement(55238..55750)
                     /locus_tag="P5516_00700"
     CDS             complement(55238..55750)
                     /locus_tag="P5516_00700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRT90989.1"
                     /translation="MNEILSPAVERIQQQFKAQTSEFRGEVTLLISADANVEAATVLR
                     DEFAFDMLSVVTAVDYCPQTNPRFHLVYIFYSTANHAYLNVRVPLDGDAPTIRTLETV
                     YPSANWREREVWDLFGIRFEGHSDLRRLLMPPDWEGHPLRKDYPLGYEEVQFTFNFDE
                     IDIRKPKGVL"
     gene            complement(55758..56240)
                     /locus_tag="P5516_00705"
     CDS             complement(55758..56240)
                     /locus_tag="P5516_00705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRT90990.1"
                     /translation="MGVEPKSGSSGVVTTSLDKMVNWARTGSLWPMTFGLACCAIEMM
                     AMQASNYDMSRFGMELMRASPRQSDLMIVAGRVSQKMGPVLRNLYDQMPDPKWVIAMG
                     DCASCGGVYNNYAVYQGVDEIVPVDVYVAGCPPRPEGLIHGILTLYEKIRTEKVSDWK
                     "
     gene            complement(56231..56587)
                     /gene="ndhC"
                     /locus_tag="P5516_00710"
     CDS             complement(56231..56587)
                     /gene="ndhC"
                     /locus_tag="P5516_00710"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRT90991.1"
                     /translation="MPFEYIPIVILLALSIAVGIIVLLLGRYLGPYRPTKKKGEPYES
                     GMIPYGPGTRQMPVRFYLVAVLFILFDVEVIFFLPWAVTFQKLGLFAIIEMGIFVTIL
                     MTGFVYLWKKGAMEWE"
     gene            complement(56848..57465)
                     /locus_tag="P5516_00715"
     CDS             complement(56848..57465)
                     /locus_tag="P5516_00715"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013352894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRT90992.1"
                     /translation="MNKVFELPQLKFAFDALEPAIDARTMEIHHGKHHAAYVANLNKA
                     LEASPEFFSMPIEDILKGINTVPETIRTAVRNNGGGHANHSLFWDVLNPGGKSAPSGA
                     LLKAIESAFGSTDAFMEKFTAAATTRFGSGWAWLVLDGQKQLQVYSTANQDSPYMDHH
                     IPILGLDVWEHAYYLNYQNRRPDYIKAFWSVVNWSFVEQQYEKAR"
     gene            57778..59256
                     /locus_tag="P5516_00720"
     CDS             57778..59256
                     /locus_tag="P5516_00720"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT90993.1"
                     /translation="MKRLLKWYELLFLNSYQLGLSIASNVISPLLLPALVLVFMPPEK
                     KNTSTALVYVTGLAVAMFIQPVMGMLSDRSTSKMGRRRPFIIVGALLNILFLGVMGAS
                     LWFKSSPLNAFLQSSFGITASLAVLLLGIILLQFSSNISQAGQQGLIPDIVPEQQRGR
                     ASGIKSVMEMVPAALVLLISPLIDRQKFWLVILILMLSFLVTMLITVAGTREEPLQEK
                     PPRAEDRPVLRMLLLTVIFVGITQLALLLVKGIGTWLPQDSTSLALRIVLVGLAGLVG
                     MAGSIFIGVYFGARVGIGKEANTHKSFIWWVVTRLMFLAAISSVRNFAMFFVKDVLQV
                     PNPATITTYLTAVIFIFLLISSIVGGILADKIGRKKLLAAAGALAVLGTIILVFSRTI
                     PMVMFAGVFLGLGTGTFMASNWALGTDLVPQKDAGRYLGISNLAGAGAGIIGSSIGGP
                     LADYFNAIQPGLGYPVIFALYGGLFLLSVLLLPRIQAETSLS"
     gene            59261..61273
                     /locus_tag="P5516_00725"
     CDS             59261..61273
                     /locus_tag="P5516_00725"
                     /inference="COORDINATES: protein motif:HMM:NF027792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5054 domain-containing protein"
                     /protein_id="HRT90994.1"
                     /translation="MERSVHLIFKTHLDVGFTDYAAVVVRNYFKKYIPAALRLARELR
                     QSGSDRFIWTTGSWLIYEYLEQASSAERGQMEEAILAGDIAWHALPFTTHTELMDAEL
                     FRFGLSLSRTLDQRFGRHTIAAKWTDVPGHTRAIIPLLAEAGVVFLHIGVNPGSTVPS
                     VPSLFRWQDPGGAELLVMYESGYGSVFEIPGLEHSLAFGHTMDNMGPQSHEQVLDVYR
                     EVRSRQPGARVFASTLSDFAAQLVRVRENLPVLSQEIGDTWIHGVGSDPIKVARFREL
                     LRLRAEWLASGRVTRSEPSLINLNRWLLMVPEHTWGMDEKTWLNDHENYTVKDFNLAR
                     GQPNFQRFEASWVEKRAYPQLAVNALGGGSLADEARARLESIRPRVPDLSAWQPIEPA
                     QAKLENPSLAVTFEQQKGSLDQLVDRTTGQSLVVRPGEFSVFSYQTFSSEDYERFFNT
                     YVLPQQRHGWALEDFCKPGLRPEDSPSRCWLPLVKGIFKRSTPGVEHILFYLTFEDEA
                     LHQFGAPRNGYLQYSLPSQEQRLHIDLQWFNKQASRMPEALWMTFRPEHHPDAEWSIE
                     KLGREIPALSVVENGNRHLHGSGRYVTCRALGSELRISALDSPLVALGARSLLDFNND
                     QPDTRQGAHFCLFNNLWGTNFPMWFEEDCRFRFDITLNPTKEERNA"
     gene            61266..62012
                     /locus_tag="P5516_00730"
     CDS             61266..62012
                     /locus_tag="P5516_00730"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRT90995.1"
                     /translation="MRELSLPGRAKPYVMAHRGNRVLCPENTLSAFLKAFEEGADILE
                     TDLHLSADGVFMCIHDATVDRTTNGKGAVNSLTLDELKQLSASYNFKGFENERIPTLA
                     ETAGILPPGVALALELKTDRFLEEEVCRRLVAELRSSGILERSLMLSFSLPRLQAVKA
                     VDPSIPIGWITMSQLTPNPLPEVLGPFWPGVFLNPFFVRRAHQRGQLVCPLDPTPDRR
                     LWYYKLLNCDALVTDNPGATLEVLKRISFK"
     gene            62036..63031
                     /locus_tag="P5516_00735"
     CDS             62036..63031
                     /locus_tag="P5516_00735"
                     /inference="COORDINATES: protein motif:HMM:NF039187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HRT90996.1"
                     /translation="MSVNQKRVSLFLPVILIGAGVILLLRNLGFLPAFNWSVLLRLWP
                     LALVVLGLDLLFGYRAPWAGGLIGLLTVAAVAVFLFFSPVMENNPPAGIKTEVVSKPL
                     ENTAHVQYYLDTSYPPVTISALPDSTDLVKATIVHRGQVHFDVKGDTDKTVTLSTSTE
                     PGPWFSYDLGLGGQKWDIGLNPSVPTTLNLDGGSGALDLQLEGITLEALRADFGSGAS
                     KITLPDSPQPYQVDIESGSGSVELALPTDTDVTFTLESGSGAVTVFLPLTSAVKIEVM
                     DNGSGTVHVPAGYIAAAGNTADHGTWQSSNFSSAAHTILIRVKDRGSGNLFFKTR"
     gene            63129..63587
                     /locus_tag="P5516_00740"
     CDS             63129..63587
                     /locus_tag="P5516_00740"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRT90997.1"
                     /translation="MNKLEHWLKTLKEDGCRITSPRRVILEVLIDSPRALQPAEVHAL
                     CRQTHPGIGLVTVYRTLERLESLGLIQRVHREDGCHLLMPAADGHEHYLVCTTCRRTI
                     LFKGDNLAPLFTKVESRTGYQVSEHWLQLFGTCPDCQLNSQQVTPKVNHA"
     gene            63580..64518
                     /locus_tag="P5516_00745"
     CDS             63580..64518
                     /locus_tag="P5516_00745"
                     /inference="COORDINATES: protein motif:HMM:NF013465.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HRT90998.1"
                     /translation="MHKKKSLLLLLSFIILLASACTGQITPTAKQLPVVATTSILADV
                     VSRVGGNLVSVTTLVPPGVNEHEYQPSPRDIAAVSDAALVFEVGLGLEQFMDAIIRNA
                     SDDITPIEVSSGISTQELLIPHDANTAEQQHEHTTDPHVWLDPANVIVWTENIAAALS
                     ARDPAGSGTYQANAAAYIAELKALDEWIRSETARIPQEKRLIVTDHMLFNYFAEKYGF
                     TVVGALIPSYSSLAQPSAQEIAALEDAIRQYNVRVILVGNTVNPTLAARVAEDTGTAM
                     VQFYTGSLSNADGPAATYLDYMRYNVKTIVNALSGK"
     gene            64524..65366
                     /locus_tag="P5516_00750"
     CDS             64524..65366
                     /locus_tag="P5516_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HRT90999.1"
                     /translation="MNSVAQPTANTITPRSHPVHIPGEPILRVEHLSLAYEGVVALQN
                     VTFQLITGERMAVIGPNGAGKSTLFKIIAGLLKPGSGTVSVFGAEPSGHICISYLPQH
                     NHVDWNFPVTVGEVVMMGRTRKIGFFRWPSAGDREIVSRALRTVGLHAHAHRQISQLS
                     GGQQQRMFIARALAQEADLMLMDEPVTGLDTPSQEELFTVLDTLREEKVTVMFALHDL
                     KLAATCFDRVMLLNRRLVGIGKPADVFTREHLSKAYGDRAQVLENGHGLSMVDDTCCG
                     GREE"
     gene            65363..66223
                     /locus_tag="P5516_00755"
     CDS             65363..66223
                     /locus_tag="P5516_00755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HRT91000.1"
                     /translation="MIDWLWTPLQYAFMVRGMLAAVIVGMVCAVLGTYVVLRGMAFFG
                     DALAHAVLPGVAVGYLLGGGARGPVFWWAMATALLSAFGIGAVSRAARIKEDTSIGII
                     FAGMFALGVALISMVRNYTADLTHFLFGDVLGVRDTDLLLTAVFGALIMLFILTFYKE
                     FLVISFDPTLAATLRLPIRWLDYLLIALIAMSIVVSLQTVGVTLMVAMLITPAAAASL
                     LTRRLSRMMLIAALIAIFSGISGLYLSYYLNLASGSAIVLTCTAVFAIIWGITALARR
                     YSRARATENM"
     gene            66261..66779
                     /locus_tag="P5516_00760"
     CDS             66261..66779
                     /locus_tag="P5516_00760"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRT91001.1"
                     /translation="MSDQTKQWSTPPEMSIDPEKSYTATFKTDLGSIICSLHAKKTPR
                     TVNNFVFLAREGFYDNTIFHRVIPDFMAQGGDPTGTGMGGPGYKFKDEFASSLRHNAP
                     GILSMANAGPNTNGSQFFITHVPTPWLDNKHSVFGQVIKGLDVLLSIPARDPSQRNSP
                     AVKLLTVEIAEA"
     gene            67032..67105
                     /locus_tag="P5516_00765"
     tRNA            67032..67105
                     /locus_tag="P5516_00765"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:67066..67068,aa:Arg,seq:gcg)
     gene            67168..67980
                     /gene="surE"
                     /locus_tag="P5516_00770"
     CDS             67168..67980
                     /gene="surE"
                     /locus_tag="P5516_00770"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRT91002.1"
                     /translation="MKKPQILLTNDDGIHSPGLWAAARKLAKLGYVTVAAPREQHSGA
                     GRSVPAWTDGEVKPTTLQIGDQEWTCYAVGGSPAQSVQYGVFAILQQKPDLVVSGINY
                     GENPATDVSLSGTVGAGLEAASYGIPALAVSLELENEDYLGYSRATDFSTAAVFTRRF
                     AKLLLARQMPEDVMVLNVNVPVSATPDTPWRVTRLGKQAYFVPFLKQPEKAGAIPRID
                     ASRNPHPSDLGDPTTDVHVLLRQKMVSVTPLSLDLTSRVDLEELQRSFGTLD"
     gene            complement(68026..69102)
                     /gene="ychF"
                     /locus_tag="P5516_00775"
     CDS             complement(68026..69102)
                     /gene="ychF"
                     /locus_tag="P5516_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HRT91003.1"
                     /translation="MRLGIIGLPQSGKTTLFNALTRATQPTGASGKIEIHTAVVDVPD
                     RRVDVLSAMFKPKKTIYAKVTYVDIAGLDGSASKSGISGALLNQLTQMDGFIQVVRAF
                     TDENVAHVHESINPQRDILNMDSEFVLNDLIAVERKLERLEEEKKKGAGREKGLIERE
                     HALFTRLQEILAGEVPLRDVEISEEDEKLLSGFGFLSRKPMMIVLNLAEGQAQPVVDY
                     THQNSRVISLQGKLEMDIAQLSADEAALFLAEYGIEEPSLSRMIRLSYDLLGLHSFFT
                     VGPDECRAWTVKRGATAPEAAGEIHTDLQKGFIRAEVVHYDDLVALGGMNEAKAHGKL
                     RLEGKEYVVKDGDVLNILFNVTKR"
     gene            69155..69236
                     /locus_tag="P5516_00780"
     tRNA            69155..69236
                     /locus_tag="P5516_00780"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:69189..69191,aa:Leu,seq:caa)
     gene            complement(69321..69899)
                     /locus_tag="P5516_00785"
     CDS             complement(69321..69899)
                     /locus_tag="P5516_00785"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT91004.1"
                     /translation="MAKKPVAVQKWEYTPLTPARWEDFELLFGPNGACAGCWCAWFRM
                     TSREFSASKKEGHKELMRTIVHSGVEPGLVAYADGVPAGWVALGPRESYKRLATSTVL
                     GPVDDQPVWVLPCFFIHRDYRCQGGMEKLLMAAIDYAKSKGVKILEAFPVEAEGKVSP
                     VHLFTGKASVFRRLGFKQVAMRENRPILRLAL"
     gene            70068..71195
                     /gene="dnaN"
                     /locus_tag="P5516_00790"
     CDS             70068..71195
                     /gene="dnaN"
                     /locus_tag="P5516_00790"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HRT91005.1"
                     /translation="MKVTVTQQHLAHGLSIVSRAVSPRTTLPVLANVLLATDEGRLRL
                     SATNLELGITCWIPAQIEEEGSITLPSRTLVDLVSTFPPDTVHLTLNDRTQTLNLVCN
                     SSVHDIKGIDAQDFPPMPVTDPEEGFDLNLADFKDMIQQVAFAASTDEARPVLQGVKM
                     EVVENHFTLAATDGFRISVRKESLSNPVEQELSVIIPARAMNELARIFSEVDKSVRMV
                     VPQGRGQVIFRLKDAELVSQLIDGNFPDYKAIIPKSAKTKTIISTPAFLKACRQAEVI
                     AREGNNVVRLDIRPEEEGPGKVEINAVTEETGQSDISVEAHIEGSGLLIAFNVRFLRE
                     VLDVIHSPNVALETNANNSPGVIRPMGEEDFVHVIMPMHLG"
     gene            71261..73591
                     /locus_tag="P5516_00795"
     CDS             71261..73591
                     /locus_tag="P5516_00795"
                     /inference="COORDINATES: protein motif:HMM:NF013491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HRT91006.1"
                     /translation="MDFSPNTSERLLNITLALAQYPILAGRIRARMREELFTRQITDA
                     KRFEARVHENAIKSQGLEGVSNPYSEESSDLWERRKSIVRDHLTDLLFSRHLTFELLE
                     SLIKEVLNERGISSDEMLLSINPELAPLDLVFEQALLIEGMPEEKRARYIARLEESKV
                     VLIRTLISDQLRYINIAKEWLSIQDLAEIRRRKIGGGRIGGKSAGMILAQHILRNNSD
                     LSDLPCLSMPESFYVGSNEFYTFMSINNLLKWNDQKYKTETEMRDDYPHIEEEFLKGT
                     FPPDILDQLQTLLIKIGKQPLIVRSSSLLEDNFGTSFAGKYESVFCPNQGSLAENLKA
                     LTRAIAQVYASTLNPNALLYRRSKGLLDYDERMALLIQWVEGDQYGDYLMPHAAGVAF
                     SHNLYRWSPQIRSEDGFIRLVWGLGTRAVDRVGNDYPRLIALSHPLLRPSNDPRSIRR
                     YSQQYVDLIDLKKNQFTTMPIHQVLAADYPPLRYLAQVDEDGYFESLRSTLISNVQNL
                     VLTFDVLIQRTPFAERMKTILKSLEQAYHAPVDVEFTVNLTENSVGSPQLCITILQCR
                     PQGFLIETEVERIPPDLTPEEILFSTDFMVPQGKVSAVDWVLYVQPEAYFALKTNAER
                     AALARTIGKLNDVLKDESFICIGPGRWGSSNADLGVPISYGDIYHARALVEMAGEHCG
                     LPPEPSLGTHFFQDLLESQIYPLALQLDDPATVFNRSFFDHAPNRLNELLPEAAGFAG
                     CLRVLRISDSYPGQTLRLIMNGEIGRAAGFLVNTQE"
     gene            complement(73637..74626)
                     /locus_tag="P5516_00800"
     CDS             complement(73637..74626)
                     /locus_tag="P5516_00800"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HRT91007.1"
                     /translation="MISNIPLLITYALSLIVMIGLPVLLAFLVVRRFNVSWWVVLAGV
                     LTFLASQALRIPLVTGINTLFSKGILSVPAAQWVPVVNAVLAGLLAGICEETARWLGF
                     KLMQKKTERYGSAFALGVGHGGAESILIAVLSIAIPIFTVLFYNPGAQIAKGVSTNDV
                     QYMLFQISRFWLDPWHAGLLPGVERVIAISTQIVLATLVWKSIADRSFTWFGLAVLYH
                     MVIDALAVFLRSKGWSLWSIEGVLAIFLLLNIYLLYRFWKEEAEIEDEVQTIDGEEDG
                     YDEDRDEDDEDNDEDPSGEEQAQVEPLAVESDEQPFSEDADEEAAGTVEEDPR"
     gene            complement(74782..75612)
                     /locus_tag="P5516_00805"
     CDS             complement(74782..75612)
                     /locus_tag="P5516_00805"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRT91008.1"
                     /translation="MNWKTILTIAKKDIKEASQNKAVWLPILIVPAIFVLIIPAALIV
                     GFSSAGTALESPLRDPDFAMFLNRMPPFMIELIRGLNEVQSGIVLVLGYLFAPFFLIM
                     PLMFSTVIAAESFAGERERKTIEALLYTPATDAELFIGKVLAGFIPAVLVTLVSFAGY
                     TIVVNATAYPVMQRLWFPLPSWYPLVLWVSPAISLLGIAFTVLISAKTQTFMGAYQTS
                     GSLVLVVLALLAGQATGVVYLSVLVGLLIGLAIAVVDVILFSFAVRAFNREQLLTATS
                     "
     gene            complement(75609..76550)
                     /locus_tag="P5516_00810"
     CDS             complement(75609..76550)
                     /locus_tag="P5516_00810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91009.1"
                     /translation="MQHAIVIEHLSHRFNSRYALKDLSYEVRQGEIFALLGPNGAGKT
                     TTIRLVNGLYTPTSGRINVLGMDSVRQGSQIRQQTGVLTETSALYERLSARENLLFFG
                     RLYGVPESLLLKRTDEILQFFGLKNRESERAGAYSKGMKQRLALARAFLTHPRILFLD
                     EPTSSLDPESTQQVHELIESLCRQEENTVFLCTHRLEEAERLADRVAILNSGRLLALG
                     SLPELTQQFNHGLWVEVDLLQPLQNDPSSLRVRGVIDVQVSERKIRAQVAEEEIIPSL
                     VATLVAQKAQIVSVQPQKVTLEEIYFKLQSQAQEGAV"
     gene            complement(76672..77109)
                     /locus_tag="P5516_00815"
     CDS             complement(76672..77109)
                     /locus_tag="P5516_00815"
                     /inference="COORDINATES: protein motif:HMM:NF012610.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRT91010.1"
                     /translation="MKVSDFTVDFRSGIPAYIQIVEQVQHKLVQGLLKPGEQLPTVRH
                     MAAHLRVNFNTIARAYRVLDNAGLISTQQGRGTYILERLEENRQQQLKRGALLTQARR
                     FFESLARQGYQPQEVEECLRDFLHSWKNTPGVSSSKIGMREME"
     gene            77287..77784
                     /locus_tag="P5516_00820"
     CDS             77287..77784
                     /locus_tag="P5516_00820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rubrerythrin family protein"
                     /protein_id="HRT91011.1"
                     /translation="MSEIYDNLMAAFAGESQANRKYLAYAKKAEEEGHTQVARLFRAV
                     AHAETIHALNHFRYAGEVGSTAENLNAAAAGEKYESTEMYPAFIENAKKAGDKKALNS
                     FHWANEAEKVHEMLYREALASLGQPGEEYDYFICPVCGYTEKRSAPEKCPVCGFPGSR
                     FERIS"
     gene            77982..78860
                     /gene="pdxS"
                     /locus_tag="P5516_00825"
     CDS             77982..78860
                     /gene="pdxS"
                     /locus_tag="P5516_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRT91012.1"
                     /translation="MVGSFEVKKGLAQMLKGGVIMDVVNADHAKIAEDAGACAVMALE
                     RVPADIRADGGVARMSDPDMIQKIMESVSIPVMAKCRIGHFVEAQILEAIGVDYIDES
                     EVLTPADEAHHIYKHAFKVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGDVVEAVR
                     HARSVLGEIRRLQQMPEEEVMAFAKEIGAPYELVTEVRTLGRLPVVNFAAGGIATPAD
                     AALMMQLGVDGVFVGSGIFKSGDPAKRAAAIVKATTHYNDPKILAEVSRNLGEAMVGR
                     QISAMPEEEKIATRGW"
     gene            78868..79446
                     /gene="pdxT"
                     /locus_tag="P5516_00830"
     CDS             78868..79446
                     /gene="pdxT"
                     /locus_tag="P5516_00830"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HRT91013.1"
                     /translation="MKIGVLALQGGFAEHQAVLEALGVEVAQVRLPQHLAGLDGLIIP
                     GGESTTIGRLAVDFDLMQPLRLFGREHAIWGTCAGAIFLSKNAGREQPLLGLMDITIA
                     RNAFGRQAESFEADLDVPALLQVDPLDRPFHTVFIRAPLIESVGQGVEIFSQLEDGRI
                     VAARQGHLLATSFHPELTGDNRFHRYFLELAK"
     gene            79503..>80041
                     /locus_tag="P5516_00835"
     CDS             79503..>80041
                     /locus_tag="P5516_00835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91014.1"
                     /translation="MISLHKAAYLIHGNPIGIAGVLARLNPTETIYTAVMEHPENGRK
                     YFCQVIHPPSSRSARFSYLAPDLIQNAEDYLPLIDFLCFQAGEMGALNVIAEIEEAHP
                     LFETLRRAGFCVFSWEAIWRLPDERSDPGSSAQWCAPAAADLNNVRNLYQTLVPPLVQ
                     NAEPFVNGNTPRLLFKQKD"
     assembly_gap    80042..80141
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <80142..81296
                     /locus_tag="P5516_00840"
     CDS             <80142..81296
                     /locus_tag="P5516_00840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HRT91015.1"
                     /translation="DTSNEIGGDGDVPHPAVGKARRMQVSTPSLQHEVMIEAVENHNP
                     EVVIIDEIGRELEAMAARTIAERGVQLVATAHGRTLENLLLNPTLSDLVGGIESVTLS
                     DEEARRRGTQKTVLERRSPPTFDMLVELQERDRLAVHQDVAAAVDTLVRGYPLQPEIR
                     WRDAANEVHVEKAPQPAAARGMAQGTRRTYSASGEEKSEPGSRHAESRYLRNASAPSL
                     AYEQAVDLSERRPINKIRIYPYGVARNRLQQAAHRLGVPALIARDVSEADIVMTLRAY
                     YRSRQQPITEAESRGIPIYVLRANTINQMEQSLAEIFNLSEGNNQNLNEVTNQTESAI
                     QAVLNGQRWVDLPPASSSIRRIQHEMARQAQLVSHSYGKDPNRRVRIFRE"
     gene            81312..81935
                     /gene="tmk"
                     /locus_tag="P5516_00845"
     CDS             81312..81935
                     /gene="tmk"
                     /locus_tag="P5516_00845"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HRT91016.1"
                     /translation="MFITLEGPDGSGKSIQVPALAEFVRQLGYDVLTTREPGGTSIGD
                     QIREVIMSMKNKSMNPRTEILLFCAARAQIVSEVIRPQLEKGVVVISDRYADSTLAYQ
                     GYGHGLDLKVLRGILDFATGGLTPDLTLLLDVDVETGLSRRQQGGGEWNRLDDYALAF
                     HRRVRDGYLELAAAEPQRWVRIDASQAPQIVQQHMQEAVLRRLRPKV"
     gene            81954..>82372
                     /locus_tag="P5516_00850"
     CDS             81954..>82372
                     /locus_tag="P5516_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91017.1"
                     /translation="MKKLAWLVWVSLFLVAACAPVSHDSAPIPASVTPSATLPFTPAL
                     FSTPIALSPQPAVTTQPTVAFDRSPAATCEVVSLVSAGVTGDFILPPEEQADWSYGSP
                     SPAVTIISYCQYQLQACRLLTNSLAELQQRYPDEVRVV"
BASE COUNT        18538 a        23299 c        21699 g        18536 t
ORIGIN      
        1 gtgtttgacc aggtcaacga tttgcgcctg gatggtcaca tccagcgcgg tggttggctc
       61 gtcagcgatg aggatttgcg ggttgcagga aagcgccatg gcgatcatca cgcgttggcg
      121 catgccgcca gaatactggt gagggtaatc cttcagtcgg tcagcagcgt tggggattcc
      181 taccagcccg agaagttcga tggaacgttt acgcgcctgt tctttggtga gattcaggtg
      241 aagcatcaag ggttcagcca tctgcttgcc aatgctcatc accgggttga gagatgtcat
      301 cgggtcctga aagaccatgc tgatctgccc tccccttaca tggcggattt catcaggggt
      361 catttttaac aggtcgttcc ctttaaagaa tgcctcgccg gctatgatct tcccgggtgg
      421 cgcagggatg agacgcaggc acgatagcat ggtaacgctc ttcccgcatc cgctttcacc
      481 gacaaaaccc agggtttctc cctctttaat gtgaaaggaa acaccgttga cagcgtgcac
      541 ggtgccctca ggggtcttga agtgagtttc taaaccttgg acttttaata atggttcacc
      601 catttattga tccttgcaat aaaaatgatc acttcttaaa aagcaggtat cttgttagtt
      661 taccataaac agatggtttt ttggctggaa aaattaatcg ggaaaaatga tggtttttca
      721 taagaaatta aattagcgga tcttcattca cagaagtgct catatgcttg tctgtgctcc
      781 ccagccggat ggctgggttt tcaaagatgc ggctgcaggt aaccagctgg ttataaatga
      841 cagggcattt cacccactgt ccgttaaagt ccaccaacac atccacctgg cccagcatct
      901 catcgtaatt gctcataatc aaaccatgtt tctggcttac cacgtctttc acatggtcga
      961 aagcgaacag gcgcatttta agcgctgttt cttgcggcac ctcctgtcct tcatgcgcca
     1021 gtatgaaatc gatcacttca tccacctgct gaacggactc tctgaacctt ttgttgagga
     1081 ttgaatcgct aaagttaccc accaggctaa atgaaagaaa acaaacaacc agtggaacga
     1141 ttaaggagaa ggtacctgat gaaaacagcg cctgttcgat gagaatattc tcgattagac
     1201 cgataatcag cgaaaccaaa gcgatgaccg caaaactgat ccacaggttc tggtataact
     1261 cgtgatggaa tggatactgt aaaaaatcgc caagagcagg gttaaatata aaaatgatcc
     1321 ggggagccac cttgatcggc agccagacca ccaggtgggc aaataaaagc gcctgcaata
     1381 accaaagcaa gaggctgaac caggcgcgct caaccctcgc ggtcaaccag ccgaccagcg
     1441 cactgctcac cagcccaatg actaaacccg gaacaaaatt cacccacgga aagactccgt
     1501 gcgcgcaggc caataaaaac gcgtccactc cccaggtaaa aatggcgaac gccagcccgg
     1561 cagcaaggcc gtaaagcagc cctgcttttt gttttcgttt gctcactgct ggtttcatct
     1621 ttaccaacct caaacccgtg caaccggtga ccctggttcg ctatcgatgt gtggaggaga
     1681 gattttcatc atacaccggt ttttaaaaaa gtcaacattt cctggtttgc aacaggcgtc
     1741 acccgggcag ggggagcctc tttctggtga ttgatgtcga atgttgcggg ttattcatcg
     1801 cctacgcgca tgcgcaggta agattcgtat ctttctgcgc taaccttgcc cgcttcgacc
     1861 gcctttatta cagcgcagcc gggttcttca taatgggcgc agtcgttgaa ctggcagtgt
     1921 ttcaccagtt cgcgcagctc cggaaaatac ccatccagct cttcaggctc ggtatcccac
     1981 agcgagagtt tgcgcagccc tggcaggtca gccacgtacc cgccgccatc cagagggaat
     2041 aattctcgca ccaccgtggt gtgcctgccc ctgtctttac gcgcgctgac ctcctgcacc
     2101 gccagctcca agccgggttg tacgcggttt aaaaggctgg acttgcccac acctgaagga
     2161 ccggtgagcg ctgatacttt gccggatagc gcctcgcgca attcatctac tccttcaccg
     2221 gtgaccgcgg aagtgtagat aacccggtaa cccaacggcg ggtagatgct gaatatccgc
     2281 cgggcgttgc gcttacctac gaggtccagt ttgttggcaa caacaaccgc gcccaacccc
     2341 tgtttttcag cgatgaccag gaaacggtcg agcatgcgta aatgcggttc aggtgaagcg
     2401 caggcgaaaa ccgctacgat ctggtttggg ttggccagta agatctgccg gtatgttccg
     2461 cgcggggtgg gatccaggcg cgccagttct cgcgcgcgag gttcgatggc ttcaatcacc
     2521 ccgctgccgt cgggaaggcg ggtaatggca acgttatcac cgatgctgat cacgtctccc
     2581 tggaaagagc ggcgcttcag ccgtccgcgc agtttacaca caatcatccc cccggctgtt
     2641 tgtacgttgt agaagccgga ttggaaacgg gtgacaatgc ctttcagggt ttccgcgctc
     2701 acatttattc ggtatcaggc tcgggggtgc gggtcacata ataatctgtt tcccaccaat
     2761 agtacctgct gatctcgccg gtaaagtaag cctcgataac acgcctgaaa accggcgcag
     2821 cgtaaactga gccatcaccc gcgttttcca gaatgactgc caccgcgata tctggttttt
     2881 gcgggttgtt cgcatcagta aagccggcaa accaggagtg ggggtcttct attgaagtgg
     2941 tagcagtacc ggttttaccg aaaatcgggg tcctcaagcc agtgaagcgg gtgtaagccg
     3001 tgccgcgctg gtcacgcacc accagttcca ttccatcttt caacgcctgc agggtttctt
     3061 ggctgaccgg gaccttgtga agcacttctg gctcgaactg tttcaggaca gctccatcca
     3121 cgctggtgat cttttcgatg atttgcggtc ggtaaagcgt gccaccattg gcaattgccg
     3181 ccaccatgtt ggccgcctgc agcgggctca ccagcatatc tccctggcca attcccattt
     3241 gcaccgctgc gccttcagtg gtgggatcat tgatctgccc cacctcttcc gcgacagcgc
     3301 caatcccggt ggcttcgcca agtccaaact cgcgcgccca atcagaaaga taggtcgcgc
     3361 ccttctgtcg gtaaaggtcc agccccaggt gataaaacca ggggttgcag gaacgcatga
     3421 ttccctgcac cagcgtgagg ttgcctgaag gtggtttctt tttatcgaac gtccagtcat
     3481 tgcccacaaa gcccggcagc tcggtgtatt ggctggtaca ttcatacctc gaatctggtg
     3541 tatacaaccc cgattcgagc gctgcggctg ccgtgaccag tttaaacaca gaccccagcg
     3601 ggtaagcgga ttgtgcggcg cgattccaca gcgggcgtgc gtggtcattc accaggtcct
     3661 gcagcgccca ctggctgtta tagttttgcg gattaaaata atttgggtcg aaactggggg
     3721 aagaagccat ggcaagtaca cggccggtat ccacttccag tacaacaatg gcgcctgaga
     3781 agccatagag cgctttttgc accaggattt gcaggtcttt atccagggtg gtgtagatca
     3841 tactgggtgg ttttgggtct gtcttactga tcaaagtatc gtaggaacca tccgcgttga
     3901 ccacataaag gtcacccgcg ggtttgcctt tgaggtattc ttcaccccat ttttcaatcc
     3961 cggctgcgcc aaccttctcg tctccggcgt agcctttgcg gcggtaatcc tcatattgtt
     4021 ctggtgaaat gctcagcagg tacccgatta cctgaggggc aattccgccg tcgtaataat
     4081 aacgcgaggt gtagggattg accgccaccc cggtgaagga attcaaataa tttgcgtagc
     4141 ggttataagt ttgcgctgaa acttcgccca gtggaatata ggagcctcct ccacgctcaa
     4201 tggtatcgcg gatcatattt gaaggaatgc ccgtcataac cgtcaggatc ccagccaggt
     4261 tgcccacctg gtcatccgcg atttcatccg gaaccacccc gattgccaca gcttcggctt
     4321 cagcagcgat gatattgccg tcattatcat aaatatcacc tcgcgtgggg gaggtgagac
     4381 tgagcgcgag ctttttccct ccgctcagtt caggcatgat caatccgctt tcccactgta
     4441 tccaccattt accgtgatct gccagcaggc ccatttccat gcggcggatg atatcgccga
     4501 acaaactggt tttaaaagac acctggtaac ccaccttcgc cgaggtggga ttgaccagcg
     4561 tggtgaggat gccgtattca atttcccgta aggtcatatt gagagcgaca tccgtatagg
     4621 ttttcacgaa atccgcttca gaaatggcat cgcggctggt aggcgccagc atggcgtaca
     4681 tgccgctgta atcctgctga ttccattttt ccaggtattc cagcacggtc agtgaaacgt
     4741 caggcaattg agtggtttgc acgataggcg aaggcagctc gccgcctggt tcaatggtgg
     4801 gttgcgcgcc attgctacaa gcggttaaaa ccaaaagagc tgtgaccaga atctgccagg
     4861 gaaatttaaa atggtttatt ttcatagttt ctctttaacc cacattctcg cctgctaaaa
     4921 tgcgtttgaa taccgggcac gcataagcca gttccttctg gcaccgataa ggcacgcgct
     4981 tgcgtacccc gaaccccagt cgcctgagcg cccgctcgat atggcacagc ggcaggccca
     5041 tgacgctggc ataacaacca gagatttttt caacgggatt gaacgtttca ttctgaatgg
     5101 cgtaagcacc cgctttgtcc agtgggtctc ctgattcgat ataattgaca atttcttctt
     5161 cggtgtaagc ccgcattttt acccgcgtgg agcaaagttc cagttctgat ataccgcgtg
     5221 agggcagcgc caaaacgatg gctgtgtgaa ccatgtggtg gcggctgcgt aactggcgca
     5281 gcatgcgggc agcgtcatca gcgttgagcg gtttaccaat aatttggctg ccatctgtca
     5341 cggtggtatc cgctgccagg ataagcgcat taaaaggtgc ctggcgttca caggcacggg
     5401 ctttttcctg cgccaggcgc gaggcatagg ctgaagggtt ctcaccagca cgtggtgttt
     5461 catcaatagc agccatggtt gttgtaaaag gcacctccat ccacgccagc atttgccgcc
     5521 gtcgcggtga attggatgcc aggacgatca ttggttcact catagaaaat attctaacac
     5581 agcccctgcc ggtggggcgt ttacagtctt atcgtgtaaa atggactata tcatttttcc
     5641 gggagggatg aatgcaagca cttaaagagc gtgtggccgc ggaaggaaaa aacctggggg
     5701 atggtattct caaggtggat agtcttatca atcaccaggt ggatcccaag ttaatggacc
     5761 tttgcggatt ggagttcgcg cgccgcttcg cgctcaccaa cccgacgcgc attttaaccg
     5821 ctgaaatttc cggcattgcg ccagcattga tgaccgcccg tcacctcaac ctgccggtgg
     5881 tgtacgcgcg taagagccgc ccggtaacca tgcccgatac ggtgtacctc accatcgctc
     5941 cttctcacac caaaggtaac aaggtagagc tgatcatctc accggaatac ctgaaacccg
     6001 gagagaaagt gctgatcatt gatgattttc tcgcctctgg ggctaccatt ttggggctgg
     6061 tacggctcac ccaggctgcc ggcgccagca tcgtgggaat cggcaccctc atcgaaaaaa
     6121 ctttcgaggg cggaagggaa gcgctgcagt acctcaaagt ccccatcgaa gcgctggtgc
     6181 aaatcaccag catggaagat gggaaaatca tctttgctcc gtagtctatc gttcgcttat
     6241 gaaaacagcc atcgccatac tggattttgg ttcacagtac acacagctaa tcgcgcgaag
     6301 ggtccgagaa ttgcaggtgt attgtgaaat ttacccatgg catactcagc cagaagccat
     6361 tcatgcatta aaccccgccg ggtttattct ttccggcgga ccgtcttctg tatatgaaga
     6421 gaacgcgcca acgttgccgc tgttcatcct gcgctccggg ctgccggtgc tgggcatctg
     6481 ttacggtatg caattgctgg ctgaggcttt aggcggtatg gtgagcgcat ctccgcagaa
     6541 agagttcggt ctggcagaaa tcgaagtcac cagttctaac ccgctgctta aaactggcag
     6601 acagactgtg tggatgtcgc acggcgacca ggtgaaagaa atgcccccgg gtttttaccg
     6661 gctggcttcc tccgccagca ctttcatcgc cgcgatggct gatcctgcgc gcggtatcta
     6721 tggtgtgcag ttccaccccg aggtgcatca caccccgcgc gggatagaaa tactgcgcgc
     6781 tttcgtgaac gatatctgcg ggttgcagcc agagtggacg ccggagaaca tcatcgcgtc
     6841 atctgttgag cgtatccgcc gccaggtggg cggcgcgaac gtgctttccg cggtcagcgg
     6901 cggagtcgat tccaccgtgg caaccacgct ggtacatcgc gccatcggca accagttgcg
     6961 caccgtcttt gtcaacaccg gcttactgcg gttgaatgaa gccgaacagg tggagaaggt
     7021 actcaaacaa catcttggta tcccgctcgt tacagtagac gccagcgccg gttattttga
     7081 cgctctgcgg ggtatcacgg atcctgaaga aaagcgccgt gtcattggcg cgaagttcat
     7141 cgaggtgttc gagcaagagg ccgcccgcgc cggcaacccg ccgttcttag tgcagggcac
     7201 aatttacccg gatgtggtgg aatcttcttc ggcgcagggc agccaggcgc agcgcatcaa
     7261 gtctcatcat aatgtcggcg ggctgcccga aagtatgaaa ttcgagttga ttgagccgct
     7321 gcgctatctt tttaaagacg aagtgcggcg cgtcggcgaa gctctgggta tcccttcgca
     7381 gctcgtctgg cgccagcctt tccctgggcc aggtttggcg gtgcgctgcc tgggcgaggt
     7441 gacccctgaa cgggtgcggc gtttacagct agctgacgcg atcttccgcg aagagctgga
     7501 aaccgccaac ctgttaaacc aaaaacccgc gccgggctgc gcgcttttgg cgcaggcttt
     7561 cgtggtgttg ttgcctgttc gttcagtggg agtgatggga gatcggcgca cctaccagga
     7621 aaccgccgcc ctgcgcgcgg tgacttcaga agattttatg accgcggatt gggcacacct
     7681 gccttacgac ctgttggggc gcacagccaa ccgcatcgtc aacgaggtgc ccgggatcaa
     7741 ccgggttgtt tacgacatca cctctaaacc gccatccacc atcgagtggg agtgattctt
     7801 attaacagcg gggttttcgc ttacaatagt tgataaggaa cattctgagc atgcgcttac
     7861 caagcaggtt catcaccttc accctggtca ttatgctgct ggcggtggtg ttttcttccg
     7921 ccgcggggca aaaccctggc agcatcacgg ttaaaaaatt ggataccagc gcgtacccgt
     7981 tgatcagcgc cactttcaca gcccttgacc ccagcggcgg gtttgtgaaa gacctcaaaa
     8041 ccggtgaact gtggttaagc gaaaacagcg ctgctgtcac ctcttacacc ctcgagatgg
     8101 aacaggtggg cgtgcgtttt tttgtcgcca tcaacgaagg ccccacgctg gataaccgtt
     8161 tttctggagt gatgcgcatc gaccgcatca agaccgcgtt gttcaattgg gttgcggctc
     8221 agccagcccc tgcgctggac gaatttcacc tggtggtcaa cgagggggcg cttcaattca
     8281 acccgcttga accctccagc tggtacgccg cgctcgatca ctatcagcca gacctgaaga
     8341 ccgccactcc cgggatggcg agcctttctg ccgcagtgga taacgcgttg aacaacagcc
     8401 tgcaatctcc caaggccaca gcactgctct tcatcacccc cctgcccacg gaagtgcagc
     8461 tcgccgccat gcaagagatc attgcccgcg ccaaaagcaa caacctgcac attttcatct
     8521 ggttgattgg cccacaggat tacggtggca gtgatacggc tgtgcagttg gagcgtatcg
     8581 cgcgggatac agggggacag tttattattt tttctggggc tgaagaactg cctgccattg
     8641 ctgatttgct cgatccattc gaattcgtgt atcacctcag ctataaaacc gccaacaaca
     8701 tcagcggaga gtacagcctg gtgcttgaaa tcaagagggg cgagctggtg ctggcttcag
     8761 aaccggtaac atacgagctg gcggtatcac cgcccaaccc catcttcctt tcgccaccca
     8821 gcactatcga acgtagttgg agcgagacca aaaaaaaatc tgcttctgtg ttgatcccgg
     8881 agaaggtcaa cctgaaaatc ctggtcgagt ttccagacgg gatgcagcgg gagctggtga
     8941 acagtcgtct tttcgtggat ggtggcttgg cagatgaaag gagcgccccg ccctttgaca
     9001 cattcacctg ggatctctca aagtacaccg aaagcggcct gcacaacctg caggtcatca
     9061 tcgaagacgc tgccgggtta acaggtcaaa ccatccagct accagtagaa gtgaaggtcg
     9121 ccgagcgaaa aatcaccgct gcggagcgcc tgctcgagca gcttacgctg gtcaatggca
     9181 tcatcatggg cgttatcctg ctggtgcttc tcatcgggct ggtattgttg gtaaaaaccc
     9241 tgaaaaagag aattaaaaag agacccgtca aaaaacccgc tgatccggtc acgcagccgg
     9301 taaatattga aggagaatac accctcgcgc cgccacaacc ggagactacg atccaatggc
     9361 cggtcatccg cggggttggg ctggcgcccg ctcgattgat ccagaagcgc tctgctgcgc
     9421 cggtggtgga ttatctgcct gaaattccgc tgggaaacga tgaggtgctt atcggctctg
     9481 aacgccgtaa agcggattac gtcctggtgc acagtacggt tagcccgctg cacgcgcgca
     9541 tctataaaga cgctgacggc aattatcgca tcgcggattc aggttccgcc gcgggcacat
     9601 gggtcaacta cgcgccggta tccaccaggg gggtacagtt ggagcatgga gacctggtgc
     9661 agttcggccg gctggcgtat atttttgaat tgcacagcgc cgcgccgcgg cgggtgaaag
     9721 tgctgccata tcaagaagac tgatcaataa tcattgtaac cgcagcagag ggcagcgccc
     9781 tccggctggt ctattttcac acctcgattc cagctgccct ggcgcgcgcc aggatttgct
     9841 ccgcccgttt taccaccggc aggtcaacca gttttccctg gtgagtgaac acaccgctgc
     9901 cgccgcgctg ggcgagctca gccccatgca caatttcacg cgcgtaagcg atgagagaat
     9961 ccgagggcgt gaacgcgcgc tgcaccggcc caacctgcga agggtgcacc actgttttac
    10021 cgctgaatcc cagctccacc gcgtcgcggc attccgcctc cagcagcgct gtatcccggt
    10081 agttgatctg caccatgtcg atggcttgca gttgaaaagc cgccgcgtgc agcaccagcg
    10141 cgctgcgcgc gtacaacagc tcgcgcgccg aatttgtacg cgtcaccccg gtatccgcgc
    10201 aaaaatcctc tgccccaaag accagtgcct gcacacgcgc gctcgcccgg cagatctccg
    10261 ccaggttcac aaacgcagac gcgctttcaa tgagcagcgc cagggcgatc tcgccctcct
    10321 gccagccttg cgactgttcg aacgcgctga tgatcttctc caccgccacg acgatcgccg
    10381 cgctatctgc tttgggaatg agaatagcat ccggtttgcc cggtaacacc gccgccaggt
    10441 cttgctcggc cctgccgctg tacagcggat taatccgcac caggcgctca gttcgcccga
    10501 aatccactcg ttccagcgcc tggcgaatca caccgcgggc ttcatctttg cggttgaaag
    10561 ccaccccgtc ttccaggtca aggatggcgc agtccagccc aagcgtggct gatttcacga
    10621 ttttattttc atcactgccg ggcacataca tcattgccct gcgcgagcgg gtcacaggtg
    10681 aagatattgg catggtcgct gctcctgtta gaaatattgg cttgcgataa aatgatacta
    10741 catggaggaa aacccgttga ataagcccgt gaaaacgctt ccagcaggtt acaccctggc
    10801 cggctccgtc actctcacca gaaccgctga tctccttcct atgcttatct ggagcctggt
    10861 gctgcccgcg cttttactcc tgctggtggt gacggtgttg tgcctcaccc atcaaatcgc
    10921 cgattggaac ctgttcagcc tccattcagt tggtcaaatc gccgcctttt ttggtggaat
    10981 gttgctggtg acgctggtga tggtcatcgc gcatgaaggg ttgcacgggc tggtattctg
    11041 gtgtgccact ggcaatctac ccaggtttga gttcaagttt tattatgctt cagccagccc
    11101 cggcggctgg tacctgccac ggcgggtgtt catgctggcc acgctggtcc ccctggtcac
    11161 catcagtctg gtatgcctgg cgctgattcc atttgcttcc ccattcttac gctgcctgct
    11221 cattttgtta gcggtgttca acgcgtcagg ctcggctggt gacctggtgg ttgcgctgcg
    11281 tctgtttagg ctgccggcag aaacgctcag cctggataat ggcacttcgg tgtacttttt
    11341 tatccccgcg cctggttgag agggttgatt cacaacccgg ttttgcttgc ccgcgggtgt
    11401 cactcgtgat aaaatgacaa tccctgcgca ggaacaagga agggtggccg agtggtttaa
    11461 ggcgcctgtc ttgaaaacag gtttggtgaa agccaacgtg ggttcgaatc cctccccttc
    11521 cgccagttac ttttcgccgg gaacaaccgc atacacgggg aggtgccgga gtggctgaac
    11581 gggatcgcct gctaagtgat tgtcgggctt taaactcgac cgggggttcg aatccccccc
    11641 tccccgccat ttttacacag gaggttttcc agcctgtgtt ttttgttcat tcgcttattt
    11701 tgtttgcgaa attatttcac attcacctgc accataaaca cttttaacgg ctcaacatta
    11761 tccttccatg gacggtaata gaccagcttg aggttactcg ttcccgcggc gatggtttta
    11821 aatttatagg taaacacgcc gcctgaaccg ataagtattg aatctgactt gtactctggt
    11881 tcgccaacca gctccaccac ggctggatcg atctccttca attcccagat gtagccggtg
    11941 gtgggtgccc cctccagagc cactgtgaat gtttgcctgt ttttcaggtc aagcgtggag
    12001 ccactatccg ccagttttaa tttcgcggcc ggcgcgctgc atgccgccaa ggcaagcaat
    12061 atcaccacaa acacgacaac cacgaaacgt tttttcatct ttacctccgg agtattcctt
    12121 atcaccaaga cgggttgggt cgtattttcg ttccacgcta gcgggcgctg caaaccacca
    12181 ggaaccagac gatggtggag attagtacca gccactccaa aaacgccatc caccagaagc
    12241 gtggacgtgc gcgcactttt tctgtgtcca gcacgttttg agcctgtttt ttcgccgctc
    12301 tgccggcgta aagcaagaaa gccgtgtagg cagccagaga taaaacacca aaaaagagca
    12361 catacagcgg aatcaccctg cgagcagccg gctgcaggat gatggccacg ctgaacagca
    12421 gcaccgtcac caatccggag cggaaatacg tcatcgccgc gattgtgtgc gggttgatct
    12481 ggttcatggg gaaaatgcca acgcacatac tggcagccgc ggtgacaatg ccggcgctca
    12541 tggcaatgta cccccagggg gtattcatat caaaacccac accgataagc atcaagatga
    12601 agagcagccc cgcgaggatc agagaggcgt tgaaaagcgg cgccatgcga gaaacaccca
    12661 gctccccaag ttcagaaatg aaatgacgtg taatcgagta acgctcaccc tctttaccgc
    12721 gataaaaaag agccgagagc agcacccctg ccaaaatcac caggctgccc gccagcccca
    12781 gcctctggta agattgtatc aaccatgcaa gcattgctga agtccctccc aagaacagct
    12841 cgtgaaattg acccttgttt ctggtattct aaaatagatg gtttgccagt atcataaccc
    12901 ataaactggc tctgacttct tttttaagtt ccttaaaaat tcgcatgcac ggagtgttct
    12961 atgtacgaaa ccttatcatc agattatgac cgttttgtta actggcataa ccgcctggcc
    13021 tatgaaatgc ctttcattga aagaatagtc aatcaaatac aaacgggcag cccgcgttcg
    13081 ctccatatcc tcgactgcgc ctgcggcacc ggtatgcacg ccatcgcact ggcgcagcgt
    13141 ggacagcagg tcagcggggc tgacctgttc ccgcaaatga tagaaataag ccgccaaaac
    13201 gctgccaatg ccggcgttca ggttcgtttc gaggttgccg ggttcgatca gctggcagcg
    13261 cagtttgggc aggctcaatt cgacctgctg ctttgcttgg ggaactcgct tcctcacctg
    13321 ctcagcccag cagatctggc tgacgcgttg gacgatttcg ccctctgtct gcgccctgga
    13381 gggatgctgc tcatccaaaa ccgcaatttt gacctggtca tgcagcagaa ggagcgctgg
    13441 atggagccgc aggttcaccg cgaaggaaac atcgaatggc ttttccagcg gttttacgat
    13501 tttgaagagg atggactgat ccgcttcaat atcgtgaccc ttaaaaatga aaatggcgct
    13561 ggctggcagt catccgttgc cagcacccgt ctgcgcccgc aattacacgc cgagttgcaa
    13621 agcgctttga gccttgccgg ttttgaaagc atacacccct atggaagcct ggcaggcgac
    13681 ccgttctcag ctgcttccag tggtaacctg gtgctgacgg cggtgcgcac ttaaaaaaag
    13741 ggaggcagta tgaagatcga gcccgtcagc agtaagtgcg acctgcaagc ctttatcaac
    13801 ctgccttatc agttataccg ccgcgaccct acctgggtgc cgccactgcg ctcagaacag
    13861 cgcgctcagt tttcgccgca gcgcaacccc atgctcaggc attgcgaata ccagctcttt
    13921 ctggccaggg agggggatga ggtagtgggg cgaatctccg ctttcatcga ccacctggcg
    13981 ctggaacact ggggacagcc cattgggctg tttggttctt ttgagtgcgt gaacgacccg
    14041 agagtctcag aagggttact cgccagcgcg cgcgcctggc tgcgcgcgaa ggatatgcgc
    14101 cggatgcgcg gtccgtggag ttttgcttca caggagtggg ggctggtggt agaaggctac
    14161 caaccagaac cggtcatcat ggcgccttac aatccgcctt attacaatga cctgctcacc
    14221 gatttcgggc ttactgcggt taaagacctg ctggtatacg tgatcgatgc ccgggaaaat
    14281 tacgtcatcc ccgagcgtat tctacagttg acgcaaaaag tgcaacagcg ttaccgcgtg
    14341 cgtgtgcgcc cagtgaatat gaaacgcctc gaagaagatg tgcttaccat tacccggctg
    14401 gcaaacgcct ccatctgtga caactggggg ttctatccag taaccgagga agaagcgcgg
    14461 gcaatggcgc gcgacatgaa gcaaattgtc aaccccgccg ccatcctcat cgccgaagag
    14521 gaaaatggca gcccaattgg cttcgccatg agcctgcctg atatcaacca ggtgctcaaa
    14581 gggcttaatg ggcgcctgtt gccttttggg ttctttaaga tgcttttact gctgccgcgc
    14641 ttgcaccagt accgtatgtg ggcgctgggg gtaattccac agtggcaggg cagggccatt
    14701 gacaccctgc tttaccgcgc cacttacgac gcgctctata ccgataaact ccgcctggag
    14761 atcaattacg tcttggaaga caacgaccgt atgaataacg ccatcagcaa gcttggtgcc
    14821 agacctctac ggcgttatcg catttacgaa gcaggtattt aagctcgccc ccaatcttga
    14881 cttatgccgg tgaagttgct atgctgaaat aaacagcacc cttcccgaat tcatcgtggt
    14941 atgttcgcca ggttaataca tcgcgcagct gttccatact gaacggtctt tatttatgaa
    15001 aggaggagca agatgtatct ccacctcgat gaagaagaaa agaagatcct gcttgattta
    15061 ttggatacct gtatttcgga cctgcgccag gaaatcgccg gcacagataa tatcaactat
    15121 aaagatatgc tcaagcaacg caagcaggtg ctcatcaagc tatttcaggc gcttcaatct
    15181 gaccagcaga cccagtcaat gacctgaaac accagtgtaa gcggcttgcc ccggtgttca
    15241 taaccttcac cgcagtcact gcgctgagat ataaaaacct ttggtcctcg ggctgaggtc
    15301 atccagcggc agcacatcta ccagctggta atcctggctg ccgagcccta atttttcagc
    15361 gtaatgcagc agcacataag ggtctttgaa cctgccatgc agctgcgcga aaggatggcc
    15421 gtcatgtggt tggggctcca ggctgctggg gatgttctcc aatatcagcg gcaggtctgt
    15481 ggtcttatcc aataccgctt tatccagcgc cacgatatcg tctgagccaa aaatgccagc
    15541 gtcttgcagg atgggcatag aggtaaagcc gaaacaatcg cacatgggcg tcatgtgtgt
    15601 ggccagcgcc agatgagtgg tctttccctt ctcaaaagtg gaaagggtga tggctgtcga
    15661 aatggcggcg gcttccataa aagtatgaaa attttccggc tgaatgagca ggcttcctgg
    15721 aggtgccacc tgctggcagc gcatgcactg gttgcaggat tcaaaatgca ggtgaagttc
    15781 ctcggggtct tttttatcaa aaaccaccgc gccaaagggg caggaatcaa caatctgctg
    15841 catcagcgac cggtcagggc aacgcccttt gaaccagtag cggtcgaaat ggctggtatc
    15901 gtgaatttga ctgcgggttt cgcctaccat acaaccaagc gcgatgttct tgaaggcagc
    15961 gccgaagccg cacatggggt gacccttgac gtgagcaaaa ttgaccaaga aactggcgtc
    16021 ctccaccata cccgcaactt tccaggcctt catatttttg tattgccatt catgatcgta
    16081 atggtagcgt tcgtgtaagc cagcgcttgg atacaccggg cagcccagca cttcgcgcgt
    16141 gtagccgcgc gactcgctgc ccgccacatc ccaatccaca tccacgatga aaggctctcc
    16201 accaccttct ttgatggcgc ttaccacttt gcgcacgaat accgggtgga tggtggaata
    16261 gccgatattg tttcccacgt gcagtttcag gcatacccat tcacctttca cccgttcgcg
    16321 caggtgcagc ctatccagta tgagatcaag tttcgctggt aatgattcgc tggcttccag
    16381 ccgtgcctgg cggggtgaac cataaaaaac agtggctgac atggcagaat tcctttcttc
    16441 cttgaggata atgaaattat atcaacgacc caaatcgcgt gccagactct tatgcccggc
    16501 gggtgggtaa gcgattacaa agaagcgata ccgcacactg ttccaccagg ttgaacctgc
    16561 cttttagcgc cgctgtgcca gtaagcaagt tacccgcgcg gtgaagacaa aagcggtacg
    16621 ctcggtttcg tatctgatgc gcccttcgcc gggggcattt taacttggcg ctaacaaact
    16681 gctgcaaacc tcttgacttt accgttttga gagaataaaa tgggctcatt gaagtcacac
    16741 tgaagttaga gcctctctcc atacagaaag ggttccagat gcaatcggaa gaaacgggca
    16801 gtaccaagga tgtacctcct ctcagtacca agaggatctt agtcctgtcc acgtacgttg
    16861 aaaagaataa ggcgcctgag agaccacaac ggtcggggcg cgcaaaggga gtacccgcct
    16921 gtctcgtatg agctgattga tcaaggcacg cttacgctgc cggccggtgc ttccgccacc
    16981 agccggtttt tgtttccggc gttaagacca caaatcatcc agtggcaaac cgcgaccacg
    17041 gtgaaccact tcatttttgg aggccggcat gcgctatttt gtcatcaaag cggaaaaggt
    17101 aacaaaaaaa tacagtcacc gcccaggtat tgagaatatt accttcgaag tggaaccagg
    17161 tgagatcttc gggctgctgg gcaacgacca cgccggaaaa agtaccctgg tgagcctgct
    17221 ggcggggtat tttcgcccta acagcggtca cttgatggtt tttggcctgg acagccagcg
    17281 cgagtcccgt caaattcgcg aacgcagtgg attttttcct ggcaattacc ccgctcccag
    17341 ccgccacacg gtggctgaat tactgcgttc acaggggcac caaccaggcg gcatgctgtg
    17401 gacggatatc cagaagctga ccgaccagtt ggacctggac ctggcacagc cagctgccac
    17461 cctttccagc cacgaccgac aggtgttcga cctgctgcag gcgttcattc cacgccctga
    17521 attgctggta ctggatgaac ctacttatga acttaaaccc gccatacagg atgtgtttta
    17581 ccggttgatt tctgaggcgc gccaggacgg gcgcaccgtc tttctcacct cgcgctcact
    17641 ggctgagatg gaacgcattt gcgaccgcgt ggccattatg caccgcggta agctggtggc
    17701 ggtagagcgc ggcattcagt tgcgttcgcg agccatccgc gtggttgaaa tgcgttttgc
    17761 cgatagcatc tcaaaagatg gctttgccga tattccgaac atccgtaacc tgaccattga
    17821 gggcaacaag cttcgttgcg tgctgcaggg agaacccgac aggctgatca agacagcctg
    17881 tcagcatcac atcactgacc tgctcagcac ccagcccagc ctggagcagg tgctcgcgga
    17941 tacgtacggg ataaagacca tctaacaagc ttgttattga taaccgctct attaccagag
    18001 ccaatgagtg aatatcgcag cctgctgaaa ggttcgatcg attttaagag ggatgctata
    18061 attcgagttc attcactcat cagcagaggt agacatgcga aacgaagaag gcctggcttt
    18121 ccacctgcat attcaacaag gcgacattgg gcgttacgtg ttgctgcccg gagacccagc
    18181 gcgctgtgaa aagatcgcgc gttattttaa agacgctcat ttcgtggcgc agaaccggga
    18241 gtacgtcacc tggaccggca gcctgctggg cgagaaggtc tcggtaattt ccaccggcat
    18301 cggctgcccc tcagcggcca tcgccgtgga ggaagccatc gaagccggcg cggacacgct
    18361 gataagagtt ggctcttctg gttcgatgca acctcatatc cacactggcg atgtggtggt
    18421 ggtcagcgcc gccatccgtg atgaaggcac cacgccgtac tacctgccta tcgaattccc
    18481 ggctgtggca gatattgagg tggtgaacgc gctgcgcgaa gcagccgaaa ggctcggcta
    18541 caaatctcac acgggtatcg cgcaatctaa agattctttc tacggcgagg tagagcgcgc
    18601 gcgtatgccc atgccggctc gcctggcgga acgctggaac gcctttatca ccggcggcgc
    18661 cgcctgctct gaaatggaat cttcagcgct tttcattctc tcgtccattt accgcaagcg
    18721 tgccggcggt gtgatgctgg tcatcaacga atctgacctc gcagaagttg acgaggacaa
    18781 ccccgccaag cacatggctg agttcgatgc tgaccgcgtc attaaagtgg cggtcgaagc
    18841 gctaaaaatc ctcatccagt ctgaccgccg ttaggtacct tccgctttga aacccacgac
    18901 actgcactcg cgcatcacca gcctctcacg tgaactggcc ctgttcatca ccgcttcttt
    18961 cgccgtgggc atggccgcca gcctggtgga ttcaacgctc aataattttc tgaacgacac
    19021 tttttcgctg aacggctttc agcgttcttt cttggagttc ccgcgcgaat tcccgggggt
    19081 gattgtgctc tttgtttcgg cggcattgtg gttcctgggc agccgccggt tgggcgcctt
    19141 cgccatgctg ctggcagccc tcggtatatt ccttatcggc ttttcttcct caagttacgg
    19201 cttgatggtg atcttcctgt tcatctacag caacggcaat cacctcttta tgcctgttgc
    19261 cactaccatt ggcatggagc tggctgctga cgggcgcacc gggcgtcgac tggggcaatt
    19321 gaacgccatc cgcaacctgg ctaccattgc gggcagcttc ctcgtcttca tcggtttcaa
    19381 attcctgggg ttcacttacc aacatagttt taccatcgcc gcattggtct tcaccgcggc
    19441 agcggtgctg atgttcttaa tgaagccgga gaaatccgcg ccgccgaaga ccttcctcaa
    19501 gctgcgcaaa gaatacacac tgttttatgc gttggctgtt ttagcggggt cgcgcaagca
    19561 aatctttatc acattcgccc cgtgggtcat cgtcactatc ttcaataaac ccacccagac
    19621 catcgccacg ctggtgacca ttggtggggt tatcggcatc ctctttcaac ccttcctggg
    19681 caaagcgatc gatcgctatg gcgaacggct tatcttgagt gcagaagccc tggtgctaat
    19741 ctttgtgtgc ctggggtacg gattctcgcg ctctgtcttt gctgaaaacg tggcgtttct
    19801 cattgtgtgt gtttgctacc tgctcgatca gatgcttttt tcggtcagca tggcgcgttc
    19861 cacttacatc aaaaagatcg cgcttgaccc gtcggatatc cagccagcgc tttccgcatc
    19921 catcagtatc gaccatgttt tttccatcag cgtggccctg ctgggcggcg tcatctggaa
    19981 caactttggc tttcagtacg ttttcttgct cggtatgctc atcgccatgg tcaatttcat
    20041 ggtggcgcgg cgtatcgtca tccccaaacc ctctataacc gaaaaaccga tcccccagcc
    20101 gatcgagcgg gattaaagcg cgcagaaccc tctccatcgc tcaaacgaaa tttggatata
    20161 atcaagttac agtatcccaa aaaaaacgct gggtaagcgt tgactgaaag aagggtaaaa
    20221 atgaaggtga atttttccat gtggaaaaaa gccctttcca ccctggtaaa gatggacagc
    20281 aaggaggagt gggatgagct ggatgtggtt tccaagtggc tgattgctac ccgctccgcg
    20341 gtgactatcg tcactttgta ctcctgtgct attggcggtt tgttggcgat caggcacggc
    20401 tacttcaacg acaactgggg aatgtccatt ctcgcctggg tcatcgttac gctggggttg
    20461 ttcatcgctc acggcacgaa caactttctc aacgattaca ctgacttcgc tcgcggagtt
    20521 gacacggaat cctacttccg tacacagtac agcgtgcacc cgctcatcca aaagttctgg
    20581 agcaaagaac aacagtttcg ctggttcgcc atcagcggca ctctcgccat gttagccggt
    20641 ctctttgccc tcttttacac ccacttctcg ctcgacatca ttattctctt cgctgtgggc
    20701 gcgctggtgc tgctgtttta tacctggcca cttaagcata tcgctttggg agagctttcc
    20761 atttttctta tctggggacc gataatagtg gcagccgttt tcctggtgct ggcgaaaggt
    20821 tcaaccgagc atgtgtggcc ggtcgcactg gctgctgtgc catgggggtt gagcgtggta
    20881 agcatcaatc tcggtaaaca catcgacaaa gcgcccgagg ataaaaagaa aatggtcggc
    20941 acgctgccag tggtcattgg cgagaaagcc gcgcgcatta ccaatatcat cctgcttgcc
    21001 cttatttatc tttttattct ctacctggtt ttcatcagcc gctatttctc tcccatcctg
    21061 ttaattgtgt tctttgccgg tcaacgcatg ctgtacgcca tcggcgtgca taccaagccg
    21121 cgcccggctg agccccctca agattggccg tactggccaa cctggttcgc gggtttcact
    21181 ttctatcaca accgactgtt cagtaacctg ctgctgatag ccattctcat tgacacctta
    21241 ttacgcttgt ttttacctgg tttttggccg gcgcaataaa gcgccgcacc tctcctaaaa
    21301 ggagcatgtt atggatattg tctttctctc gcgctggcag tttgctctca ccaccattta
    21361 tcatttcttc tttgtgccgc tcacgcttgg gcttggctgg ttcgtcgcca tcctcgaaac
    21421 gatgtacgcg cgcagcggca acctcttgta taagcgcctc acgaaattct ggggcaagct
    21481 ctttctcatt aattacgcca tgggcgtggt cacgggcatc gtgatggagt ttcagttcgg
    21541 tatgaactgg tcggagtatt cacgctttgt aggagatgta ttcggcgctc cattggcggt
    21601 ggaagggctg ttggcgttct tcctggaatc cactttcctg ggggtgtgga tctttggttg
    21661 ggaacgcctt tccaagaagc tgcacgccgc ggtgatgtgg ctcgtggcca tcggcggtaa
    21721 cctgagcgcg ttttggatcc tggcggcaaa cggtttcatg cagcaccctg tgggatacgc
    21781 cattgtgaac ggtcgcgcgc agatgaccga ctttggcgcg ctgctgggca acccgcgcgc
    21841 gctttacctt tttctgcaca ccttctttgc cggtttggtg accgcttcgt tctttatgtt
    21901 ggggttcagt atctaccacc tggtcaagaa acaagaagtg gaggctttta gaaaatcctt
    21961 tgtgctttcc gccttgattg gcctgctctc cacagtagcg gtggggggcg ctggacatat
    22021 tcagggcgtt tacctgcgtg aagtgcagcc catggctgct gccgcttcag aagcccatta
    22081 tgaaacagcc aacccggcgg actttcacct tatcgccggt attgaccagg atgggcgcaa
    22141 aaccacctgg tcagtcacca ttcccagggc attaagcttt ttgtatttct ttaaatggga
    22201 aggcgaggtg gaggggctca accaggtgca ggcgcgctac gagcagcttt acggtccggg
    22261 aaattacatc cccaacattg ccctgacctt ctggtctttc agggtgatgg tgggggtggg
    22321 gttgctgatg ggattgctca gccttggcgc gctggtcatc gcctggaaaa agtaccccga
    22381 aaagtggatt gagcgctctg gctggctggt ttggatcatt ctcctgcctt atctcgccaa
    22441 caccaccggg tgggtgttga cggagaccgg ccggcaaccc tgggtggtgc atggattgat
    22501 gcaaaccaaa gacgccgtca gccctaacct ggcacccgcc acagtgctca tcagtttgat
    22561 cggttttgtg ttgatctacg cggtcttaat ggtgattgac ctttacctgc tggtaaagtt
    22621 cgccaaaaaa gggctcggcg ccggagattt agattcggtt cccattacgg gtgaagttga
    22681 agcaaagaaa aaaggaggaa aatgatgaac ctcaatatcc tgtggtttgt gctggttgcc
    22741 gttttgtacg ctggtttctt tttcctcgag ggatttgatt tcggcgtcgg catgctgctg
    22801 ccctcgctgg ggcgtaaaga tgtggaacgt cgcgccatca tcaacactat cggtccgcac
    22861 tgggacggca acgaagtgtg gctcatcgtg gcgggcggcg ccaccttcgc ggcttttccg
    22921 caatggtacg ccaccctgtt cagtggtttt tacctgggct tttttctatt gctcttcgcc
    22981 ctcatcgtgc gtggtgtggg gttcgagttc cgcagcaaac tttccgcggc tcgctggcgc
    23041 tacggctggg attgggctat cgccgtctca agctttgtgg ccgccttctt gctgggtgtg
    23101 gtctttacca acctggtgcg cggcgtgccg attaacgctg ccatgcaata caccggcggc
    23161 ttcttcacac tgctcaaccc tatcagcctg ttgggcgggt tgacggtagt gatgctcttt
    23221 ctgttccatg gggcgaactt cctcaccctt aaactgagcg atggactgcg cgagagagcg
    23281 cgtaacgcca cgaaaaccta tttcaccgcc gcgctgttgt tcgcgctggg gctgtttgcc
    23341 gcgctgctgt tcaccccttc cgccttttct acaaagggcg ttttcagcgc tgttctgcca
    23401 gtgctagccg ccgcttcgct ggtcgtttcg cgggtaatga tgggcaaaaa gaaggaaggc
    23461 tgggcgttca tttgctccag cctggcggtt gtgctcaccg tggcggcatt gttcaccgcg
    23521 ctcttcccca acgtgatggt gtccaccaca aacccggcga ataacctgac gatttataac
    23581 gcttcctcgt ccgattacac gcttaaagtg ctctccatca tcgccgctgt cttggtgccg
    23641 gtggtgctgg cgtatcaaac ctggacctat gtgatcttca ggaagcgcgt gaaggcagac
    23701 accaagtcac tgacgtatta agctctctgt tcgcttgagc gcaggggcaa tggatcagtt
    23761 gcccctgttt tttattgcca ggttcaccgc cgcctcagcg tggatcttcg tggtagcgaa
    23821 gattggcaat acgctgtctt gcgggcgcac caggttttca atttcagtgc agcccaaaat
    23881 caccccctgc gcccccacgt ccgctaaatt gctgatcacc ctgctgaatt tctcccttga
    23941 cgactctttc accactccca tgcagagttc ttgataaatc acctcatgga tgatctgccg
    24001 ctcagcttcg cctggaacga gcacgttcag cccatgtttt ttcgccaggc ggtctctgta
    24061 aaaatcctgt tccatagtaa aacgcgtgcc caataacccc acctgcgtga ttccagcggc
    24121 tttcacagca tcagccgtgg cgtcagcgat gtgcagcagc gggatgccca gttcggcttc
    24181 caggcttggc gccagtttgt gcatggtgtt ggtgcataac aggatgaagt ccgcgcctgc
    24241 cgcttgcaga gcgcgtccag cagcaagtaa acaccgcgcc gcttcctgcc agttgccctg
    24301 gcgctgccat tcttcgatct cagcaaaatc aacggagtac agcacgcatc tggcggaatg
    24361 gaaaccgcca agccgttttt tcgtttccag gttaaggatg cggtaatact cgagggttga
    24421 ttcccagctc ataccgccaa tcagaccgat ggtttgcata ggctgtctcc tgacgacctc
    24481 tgttacctca ttttacatca gcatttgtgg ttgtattttt cgtaaatttt tgtacaatac
    24541 aattacctgg taaaaccacc agccaagcat gcgtacaacc tttgaaccgt tgcaagtcac
    24601 cagcctgaaa gaggcctgta tcacccgcct ggaggagctg atcctttccg gtgagctgaa
    24661 agccggcgag cgtctgcctg cggaacggga ttttgccgcg cgcctgggcg taagccgccc
    24721 ggtgctgcat gaggcgttag tcgacctggc agcgaaaggc ctggtcacca ttcacccgcg
    24781 ccgcggtgtg ctggtcaacg attttcgcgt ctcgggctct tgcgccatcc tctcatcatt
    24841 acttacgtat cacaacggca gccttgacgt tgatttcact gaaagtctgc tggcgatgcg
    24901 tgtgttggtg gaaactgaaa ccgcccgcct ggcagcgttg tatgttacgc cacagcaaga
    24961 gcgctccctt gaggagatca tcgccaatga agccgcctgc gaccgcgctg acccgctgac
    25021 ccgcacaagc ttggattttg attttcacct ggcggtcgcc atcgcgtcag gcaacctggt
    25081 ttacccgttg atccttaact cgtttaaaaa ggtttacacc cgattcactg gcgttttctt
    25141 ttcccattcc gctggcagcg cggtgatgga tgaggtgctg gccttccatc gccgcctggt
    25201 gagcgccatc cgcgaacatc agcctgaggt tgctgcagcg gtgatgcgcg agttactgct
    25261 gcacggcgag cagattttaa aagagatttt ataagcaggt aaacaaggag gaatagatga
    25321 gcacccagac cacagataaa cccaggtttg atattaaggc tcccaggaat ctttctgagc
    25381 gcatcagctg gctgcgtgat tattacttcg aaggggtcaa ccgaaaatgg aataacgagt
    25441 atatctcgta caccaccggc acagattggg acttccaata taacgagctt tctttttaca
    25501 tcgtccccga aacgtatttc tacatcccca ccttccgctc ctcctttaaa caactnnnnn
    25561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    25621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnngcgct ccccccggcg cagcaaaaag
    25681 gtaaaaaagg cgctcagctg cgcgccatgc tgaccgcttc caccctggcg cgcgaccttg
    25741 ctacagagta tcaggccctg ttagtcagtc tggcacacgc tgaacaagac cccatgcgca
    25801 aaacggaact agagcaaatg gcgcacaatc tttcccgtgt gccctgggaa cccgcagaaa
    25861 ccttctggca ggcggtgcaa tccctgtgga tgacacacat gctggttatg gccgatgaat
    25921 gctacccggg ccctggtgtt tcctttgggc gtattgacca gtatctcaac ccgctctggg
    25981 agcgctcgct ggctgaaggg atgcagcgcg aattcggcaa ggaaatcctc aaatgctttt
    26041 gggtgcattg caataccgcc tacgacgcta tgatccaaac cggcggtaac cagggcatca
    26101 ctgccgggta cggtcagctg ctcaccctga gcggagcggg taagggcggc gtggatatga
    26161 ccaatgacct caccttcgcc atgctcgaag tcattgacga gatgacgccc atcctggagc
    26221 cgaagcccaa cgtgcgcctg catcgcggca cgcctgatga cctgctgacc aaggtggtag
    26281 atatgatctc ctccagccaa ggagcaccct tcttgttgaa cttcgacgag cgctcaatgg
    26341 ccggcatgat gttggaagca cgcaaagccg gggtggagca tctcatccat gcggaaaacg
    26401 tgcatgagta cgccccagtt ggctgcctgg aaaacactat ggtggggaat gaccgctccg
    26461 gtaccgttga cgataacctg aacctgctca aagcggtaga gctcacgctc accgggggtt
    26521 acgacctgaa cccggtggtc gacgcgctca gcggaaaagc agaaaagccg cgtcgtgatg
    26581 gtgaaaacag cggcgatccg gccagcttca ccacctggga gcagttctgg gaagcctacg
    26641 ccctccaaac gcgctatatc atccgtaaaa gtgttgaaac atacgaacgc tctgaagaga
    26701 ttcgcgccaa gtatttccag acgccttacg tttcttgcct ggtaaagggc tgcgcggaaa
    26761 gtggtaaaga tatcaacgaa ggcggtgcgg aacttggctt tgtcactatt gaaggggtca
    26821 ccttcgccac caccgtggat tctctgctgg cggtgaaata cctggttttt gaccatgcca
    26881 tttgcacgat gaaccagttg gtgcaggcgc tgcgcgacaa ctgggtgggg cacgagaagc
    26941 tgcaagccat cgcgctgtac aaagccccca agtatggccg tgacgaggat gacgcagacg
    27001 ccatggccag gcaggtgatg gcattgtgga cagaagannn nnnnnnnnnn nnnnnnnnnn
    27061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    27121 nnnnnnnnnn nnnnnnntac ctgcgcggtt atattgaaaa tggcggcagc gcgctgcaga
    27181 tcaacatgct caacgcagac atgctgcgcg acgcgcagct acaccccgaa gattaccgcc
    27241 acctgttggt gcgcgtgacc ggttacaacg cttacttcac cgcaattggc aaagaactgc
    27301 aggatgagat catcgcccga gaaagccacc agatgtgatg tcatccgaaa acgtttctac
    27361 ccgcggcact atcctgcacc tgcaacgcct ctccacggag gatggcccgg ggattcgcac
    27421 cacggttttc ttcaagggct gcacgctgcg ctgcgaatgg tgccacaacc ctgaaagcct
    27481 ttcccggcac aggcaggtac agtggctcgc caaccgctgc atcggctgcg cgagctgtgt
    27541 ggctgcctgc gaggtgcata gtctcacgct caccgttgag ggcattgtgc gcgaccgcgg
    27601 gttgtgcgtg acgtgcgggc gctgtgttga agcctgtcca tcaggcgcga tggaaatgct
    27661 gggaaaagaa gtgagcgcag cggaactgac cgccgaactg ctgaaggacc gcgcttactt
    27721 cgaaaaatcc ggcggcggtg ttaccctctc aggaggggaa ccaaccctgc agccggcgtt
    27781 ttgcgctgcg ctgatggacg ccttgcacgc tgagggcgtg catgtggcgt tggatacctg
    27841 cgggttaagc gcgcctggtg tgttcagtga gctggcggca aaggctgacc tcattttgtt
    27901 tgacctgaaa ctgatgaacc ccgatgaaca caaacgttgg acaggcgcgg acaacagccg
    27961 tatcctgcgc aacctggaaa ttgcgcgcga tttcgtgtct cgttcaggcg gggcaaaacg
    28021 cctgtggatt cgcacgccgc tcatccccgg cgcaacaaca gcccctgaca acctggcagg
    28081 tatcagtgcg tacctcggcg catcgttcga tggcagtgta gagcgctggg agctatgcgc
    28141 cttcaacaac ctgtgccgcg acaaatacga acgcctgggc atgcgctgga cctttgccga
    28201 aacagaactg ctgaccgcgc aacaactgac acagatgggt gaatgggcca ggtcaggcgg
    28261 cttcgaccct tcgcgcacct ttgtcactgg ggcagccaaa gccagttgat tatttcgaga
    28321 aataatgctt gaatggagac cctaaaacca tgaactcctc tctttacccc cgcaacagtt
    28381 tttcagaaca agagatcaaa gacctgtccg cggcaatgaa ggtcggtatt ctgggcacgg
    28441 tgaacccgca ggggctgccg catctcaccc tcatcaccac gctcatggcg agcagcccac
    28501 aggtggtggt atggggtcag ttcatggaag gcagcagcaa gaaacatgtg ctcaccaacc
    28561 cgaagaccgg attcatggtg atggggctgg ataagaattt ctggcgcggt ttcgctgatt
    28621 attctcactc agagacttca ggaaaggatt acgcgtacta caacaacacc ccgcttttcc
    28681 gttacaacgc gtattttggt gtgcaccgcg tgcattatat ggaccttatc gctcacacgg
    28741 ggaagcaccc gctgccgatg cagcgcatta tccccgccgc ggtgatgacc atggtggcta
    28801 aaacgctcag cgcgaaacca caaaagaccc ctgtgctgaa tccctggaca caggcatttt
    28861 tcaacaagat tgataacctt aagttcctcg gttacgtcaa cgcggagggc tacccggtta
    28921 tcatcccgct tatccaggcg caggcgctcg accgcgagca tctcatctat tcattgggcg
    28981 cgtttggtga tgagctggcg caaattccac cgtacactcc agtggcagtg ttcggcatgg
    29041 cgctcagcat ggaagatgtg ctggtgcgcg gcacttacca gggaatcaag cgcgttgccg
    29101 gcataagatg cggtctcgtc cagttagact gggtctacaa ttccatgccg cccacccccc
    29161 ggcaaatcta ccctgaagtg gtcatcgaag cagtgagcga attttaaagg tcaaggaagc
    29221 attttcaaat gatgctccag gcttcagacc gcctggagcg agcgatgaaa ttcaatcaat
    29281 gaggtgtttc gacccatttc tgaccatggg tcagctttta ctgacattgc ttaataggtc
    29341 atgggtcgtt gaacttcggt tagaattatc catctatgca gagaaccatc gtgtttgact
    29401 ttggcggggt gctcattgac tggagtccct accatcttta ccgcaagttg ctgcccagcg
    29461 acgacgctgt gcgcgagttt ttggaagaaa taaagttcag tgaattcaac aaaaagctgg
    29521 acgctggcta tcctttccac aaaatgaaag aggaattgct tgaaaaatac ccgcaccggt
    29581 atcaactggt gcgcgctttt tttgaacgct ggatggaatg cactggcgaa gccgtcagac
    29641 cagccgtgga aatcatgcgc gaaattaaac gcgcgggtta cccggtgtac gggctgagca
    29701 actggtccac cgaaaccttc ccgctcctgc agcaccgcta ccagttcctg cctgaactgg
    29761 atggttacct gctttcaggg atggcaggtg tagccaaacc ggacgaggaa atcttcagga
    29821 tcttcctggc gcgttttgag cgcaacgccg aggattgcgt gtttatcgat gacacgcagg
    29881 cgaatgtcga cgccgccagg cggctcgggt tcaacgccat tctctttact tcagccggcc
    29941 aactacgcgt tgcgctggtg aaaattggcg tattgaaggc ttgaaaccat tttctctgtt
    30001 ggggagcgcc tgaaagaaac ctcgctttca caacttgtag ggcgtatacc gcgggcgcgg
    30061 acgggctttc tctgccaggc tcattctggc cctccaggtg ttactcgccc ttctgcatcg
    30121 aggcttgcgt gaaatcgctg actttcatcc cttgcgccct ggccgcttca ataattcgca
    30181 caaagagcgg atgggtttcc ccggggcggt gataaaaata aacctcgtca aaaaaagaca
    30241 ggcgtgtgat gaaactttga agcagccttt cgtaatcttc ttcggtaacc gtgtcaggag
    30301 gttggttgta ccatttgatg tggtcatcgc ggaaaaccac tccgtagcgg tcggagagaa
    30361 ttgcccagta gtgagaatgc tcattacaat acttgatgaa ctgttgaatc ccctctgagg
    30421 tgtataaccg gtcaggcgtg accttttctc cgttcagcgc aaattcaggg tctttttctt
    30481 tggaacaatg agtgaggtag atttttttca ctggttgcct tgtccctttc ttttcacgcc
    30541 gaacaacact gggaagaaga ggatgaccag cagcatcaac gcgccgaaaa ctagaaaagt
    30601 cagcgggtaa ccagccgcgt tagccagccc tccgcccagc cccaggccga taccctgacc
    30661 cacattggtg accgccatca ggatggagaa catcgatgcg gcaatggcag gctgggtata
    30721 cttcatcgcc agcgcaaagt aaatcgccag agagagcccg taggagacgc caaagaagac
    30781 gatcaccgcc cacatcatcg gtcggttggc caccatcgcc agcaatatca tagccggtac
    30841 cacgagcagg atggacagcc acagcgcttt ttggtttccc agcttatcca tcaccctccc
    30901 gccagccagc gcgccaacca cacagcctat cccccacaca gtagtgagca aaccggctgt
    30961 gctgaggtca atgcccagtt tggcgtcaag agaggggtta accaattgat tcgcccccac
    31021 aatcaccagg aaaatgacca gccccaccgc ggctactgcc agcacctgca catttttaaa
    31081 cgcgcgaaaa gctgaccatt gaaagcgcat ttcagccgtt ttctgaattt ccttgataaa
    31141 gaacaagaac gggattggca ccaaagtgag gatggccaga aagtaaaaca cgccttgcca
    31201 tgagaggtgc tgcgcgataa gcccggcaac agaagcagcc acaatcacgc ccactgaacg
    31261 cccgccgacc ataaaaccct gcagggtgcc tttttctgtt tctggtgtga tatccagcgc
    31321 cagcccatcg gtgcaggtat cataaaacgc catacccagt tggagcaaga aagccagccc
    31381 cacataaagc cagtaacttc taccggggtt gatgttggcc acaacgatga ggcaggcaaa
    31441 ctgcaccagc agaccgatga agatgtacgg tttgcggtgt cccaacccta acaggttgta
    31501 acggtcactc agaatgccaa aaaggatctt gatgacaaaa gggatgaggg caatggtgga
    31561 aaagatgccc acagccgcca tatctaatcc attatccagc atgtacagcg cgttgagaga
    31621 agcgaaatat cccaatatcg ttccctgggt aaaatagagc agcgcgaaca tcatcaggcg
    31681 agtgaacttg ctttgtgggc tcatagggtt tcctttgtaa atttttatgt caaaattata
    31741 caaataatca aacagaaaga aaacgatcaa accggaggga aaatgaccgg agattttagc
    31801 agcaagaatg tattggtcac cggagccagt tcagggattg gctaccaggc ggcgctcgat
    31861 ctggcgggca aaggggctta tataattggc accggtcggg acgtgacccg ttgcgaagcg
    31921 gcgcgcactc gtatattaca agtccatcca gaagcccaaa tcgagtacct ggtcgccgat
    31981 ctcgcttctc aaacccaaat tcatgatctg gcgcgtcagg cgcagcttct cctgatagaa
    32041 catgggaaca ggctggatgt gctggtcaac aatgccggtc tctattccag caagcgcgtc
    32101 cttacggaag atggcatcga actgacattc gcggtgaacc atctggcgcc ttttctgctc
    32161 actcatttgc tgctgccgca attgttaaac acgccgcagg gtcgcgtttt gggggtcagt
    32221 tctcagtctc attaccacgc atttctctct cccctgaagg cttaccgtcc ctggctgtac
    32281 ctcagcctgc cggtgtacgc cacatccaac ctgtgccggg tgctcttcag cgcggaattc
    32341 aaccgccgta caccaacacc tcacctgcac gcctggtcgg ttgacccggg attggtcaac
    32401 acggaaatcg gtcaaaaaga taagggtttt ctctccaacc tggtttggcg ttttcgaaaa
    32461 acgcacggta ccagcgctga aattccctcc caaaccattc tgcacctggc cttttctgcc
    32521 tttcaccata tcgccgctga cctgtactgg aaagacagcc gcccaaaaga accctcaagg
    32581 ctttctcgcg acgccgagct ggcaaaacgt ttgtgggaac aatcctgccg cttatgcggg
    32641 attgatgact acttcaaccc aggctgaaac aacgcatcga gatcgagccc atgatgacct
    32701 tttcattgaa aaaactgacc ggctcgtccg cgtaccccgc accgagtacg taaagcatcg
    32761 gcaggtaatg ctcggctgaa ttcaccgcca gttcagcggg tttcccgtgt tcctcataat
    32821 gaaccacctg ttcgtgttcg cctttttcga tccagttttt gaggtcggcg tcataggaaa
    32881 cggcccagtc ataaccagcg tctgcttccc atgtgaccat gcgcaggtta tgcaccatat
    32941 ttccgctgcc aaggatcagt atgccgtcat cgcacaaggg gcgtaattct ctgccgagca
    33001 aataatgaga ttggacggag agttgacggt taaggctcag ctgtaccacc gggatctccg
    33061 cctttgggta catgcggcag agcaccgacc aactgccatg atccaatccc cactcctggt
    33121 cttcaacgac agtggttgat ttgatttttt gctttaccaa ctcgaccagt tcgtctgatc
    33181 ccggcgcggg atactgcatc tcgttcaatt cgcgcgggaa tccatagaaa tcatgtatgg
    33241 tgcgggggtt tgccatagcg gtcacacggg tgtcccaggt ttcccagtgg gcagaaatgc
    33301 aaagcaccgc tcgcggtctg ggcattaccc gtcccaattc ctcccaggta aggctgaact
    33361 cgttttgttc aatggcgttc attgggctgc catgacccac aaataaaacg ggtaatcgag
    33421 tgacaggtat catttcacct ccttgtttgc cttttcagcg gcgcgcgctt tatgtttcac
    33481 tttgatcacg actatttttg tagggtgatt gaacggcgct gaatcaaccg gcggctggtt
    33541 tacaaccgtt tcggcataac ctgttacatc ctcccctacc gggatgatat gctcaatggt
    33601 cagcaggcca ttttcggtca gggcttccag ttcgccgatg aattcccgcc cgcttaaaaa
    33661 cagggcatta ttgaccacca ccaggtagcc gccatcggtt accaaagggc gcgctttatt
    33721 gataaggcgc gtggtttctt tcacctgatc tactcgaccg cgcggcgtga gggaaaagaa
    33781 aggcgggtcg atgatgacaa tgtcatatgt ttcgcgtttg cggcgcatct gacctgccgc
    33841 cacaaagaaa tcaaccgcgc tggtcttcat cttcgtctca tccaacgcgt tcagcctgag
    33901 ggacgcgcgt gccacctcca ggaaacgcgc gctgcggtcg atctgggtaa cggcagatgc
    33961 cccaccggcg agcgcagcca cccccaagct gccagtatag gcgaacgtat tgagcacaga
    34021 ttgattgctc gcgttttcct tgagccaggc tctcaatccg cgcgtgtcca ggtagaaccc
    34081 ggcatcctgg ttcaggcgca acgcaagcgc gtattgcaca ccgttttccc acacaacggt
    34141 atccggttga gccccgtata gcaccacccc cttcttcgcg tcaggatgcg tggaagaccg
    34201 ctgcttgagc aacacgcacc caatccaatc cagttgggca aggagatgct gctgcaccat
    34261 ctgggccagc gcgagagaat cagcggcatc cagtttatgg gtaaagacca ccagcgtgcg
    34321 cgcatacagg tcaaccacca gcggagggaa tccctcgtaa aaaccgttga ataagcgaat
    34381 ggctgaacgc ggcggctgct ccagtagcgc ctgccggtga tacagcgcgc gggtgagcaa
    34441 ccccgtcagt tcacttactt tatttcgatc catggcataa ttgtaatcgg tcaggtagat
    34501 ttaaaaatcg catatccttg cctggtcaga aggcgcccat gaaccagacc ctgcgtatca
    34561 ttcatcaccg acgttcaact cgtaagtacg ccgctacacc catcacctcc gaagaaaaag
    34621 atgccatcct gcaggctgcg ctgcgcgcac ctaccgcagg caatatgatg ctgtatacga
    34681 tcattgaggt tgaagaccag gcgctcaaag accggctgtc ggtaagctgc gacaatcagc
    34741 cgtttatcgc cagcgcctct tacgtactgc tctttctggc agactatcag cgctgggtgg
    34801 aggctttcaa ttggagcgat gcgccgcggc tggccatgga acagggtctg caaccccggc
    34861 tgccgcagga gggcgacctt ctgctggcat gctgcgatgc gctcatcgcc gcccagaccg
    34921 cggtcatcgc cgctgaatcg ctgggaatcg gttcgtgcta tattggtgac attatggaga
    34981 attacgaggt gcaccgcgaa atgttccgcc tgccgcgctt cacctttcca gttaccctgt
    35041 tgtgtttcgg cagaccttac gcagagcgtg agcacccggg gttaaccagg cgctttggac
    35101 gcaatatcat cgttcatcgt gatcattaca cgccggtaga cgcggacgcg ttccccgcca
    35161 tgctgtcgcg cgctggtcag cagtccccag atgaagtgga cgtagaagcc gccaggaaga
    35221 tggcgcaagg aatttataca cggaaattcg tttctgcctt ttcagtggaa atgacgcgct
    35281 ctgtaaaacg ctggatggaa atctggactg gtgccgaaga ttaacaggtg ggtgagttgg
    35341 atgttcccac cagccgcgcc agcggcgagg attggctgtt ctacgacgcc ccactggtgc
    35401 tgcatttttc cggcagcgct tacagtgacc cagcggattg cgcggttgtg gcttcccttg
    35461 ccatgcttgc cggagagtcg ttagggctgg gcagctgtat gctgggtttc cctggttata
    35521 tcttccgtta cagcaaaccc ctgcgcgaga aatacgttct gccgccaaaa attcaaccgg
    35581 gactgacagt ggtcttcggc taccccaggt ttaccccgcg taacgctgta agacgccgct
    35641 tcagagaagt gcgcgagtgc gcatgaatga gaagaacggt ataattaggc cctagtaaaa
    35701 aaatggtaga tgataaaacc cctgaagttg aatacccggt ggaattcccc ctcaaagtca
    35761 tcggcgtgga tgaaaacgac tttgaaccct tcgtggtaga aatcgtgcgg cgtcacgttc
    35821 cagatttatt ggaagaaaat attgtcagcc ggctctccaa caacaaccat taccgctcgg
    35881 tctccttcca ctttattgcc cagagccgcg agcaggtgga cgcgctctat gctgagttaa
    35941 gcagccacaa gcgcgtgttg atgatcctct agccttcctt tttcttgatt tcgtgaccgc
    36001 caaactgatg ccgcatggct gcgaccagtt tatcggaata cgggccatcc tgccgcgaac
    36061 ggatacgccg ttgcagcgaa gcggtgatca ccggtgcagc cacattcagg tcaatggctt
    36121 caaacacggt ccagcgtccc tcgcctgaat ccgccacata ggcttcgaca tcctcaagcg
    36181 acgagtcttc tgccatcacg ttttcgatca gttccaacaa ccatgagcgt atcacgctgc
    36241 cacgctgcca tatctgcgaa acgcggtgca ggtcgagttc aaactcccgc ttctccttca
    36301 agatttcgaa cccctctgca aaggcctgca tcataccgta ttcaatacca ttgtgtacca
    36361 tcttggtaaa atgccccgcc ccagatgggc caactctgcc ccagccctgg tcaggcgctg
    36421 gcgccagcgt ttcaaaaatc gggcgcaggt actccacggt ggcaggatca ccacctatca
    36481 tcaggctgta gccttctgtg agcccccata tgcctccgct ggttcccaca tccacaaaac
    36541 gcaaaccttt ttccatcaga acctgaccgc ggcgggtagt gtctttgtag ttagaatttc
    36601 cgccatccac gacgatatcc cccatctcca acagaggggt gagcgacccc agcacttctt
    36661 cggtcaccgc gccagcggga accattagcc acaccacgcg tggttgtgcc agattggcgg
    36721 taagctcttg caaagagcgc gctcctttca cccccagcgc ttcggcttca cggattgtgg
    36781 tttctttggt ggcgttgcca atcacgctgt gcccgccctt tatcaggcgt tgcgccatat
    36841 tcatccccat ttttcccaga ccgatcatcc cgatttgcat attttcctcc aatgacctcg
    36901 atgttggcat aattatatcc gctgccaatg gaaaaaacgg gggcccactg accagcccct
    36961 taccgcccct gtatggatga tttttcctac aaagagatgc gattctccac gcccgccagt
    37021 atgcggcgga tgttctcgtg atggcgcaag agggcaaaca gcgctgcaac caccacgacc
    37081 accacccaca tgagcggttg ctgcgtaaca atcacataca cagccatgcc gaaaacaaac
    37141 accaatgacc ccaatgagac catgcgcgtg cccagaacca ccagcacaaa aacgagtatg
    37201 cccgccaggg ttggcagggg cagcaaactg agcaggatac cagtggtggc ggcgatgccc
    37261 ttcccgccgc gaaagtttaa aaccaccgga aagtcatgtc caagaaccac cccaatggct
    37321 gcggctgcca atccggtttc tccccccagc cagtagccag cgcccgccgc agtggctcct
    37381 ttaagcgcgt ctaacaaaaa caccagcaac ccatagcgcc agcccaacgt gcggattaca
    37441 ttggtggccc cagcgttacc gctgcctttt tcacgtatat caaatctgcc aaccttgcgg
    37501 gcaatcagat aagcgggtga aaaattaccc agcgcatagc ctaacaagaa caatcctgtg
    37561 atttggagca gtttcataat acaaagtaac actgcaaccc gcggattgga tacgccgttc
    37621 tacccatcac ttttctctct cagggtaccc gttttaatgg ttgcctgctc atgctgaatc
    37681 tggatataat gttctgaaat tagggaaaag gactgacatg acagactcca catcaatcgt
    37741 aatcttcggc gcctctggcg acctctctca ccgcaagtta attcccgcct tatatcacct
    37801 gtatctcaaa ggccgcctgc cgaaatcctt cagggtgttc ggtatcgcag ggagaccctg
    37861 gacggacgaa gacctgcgcc gggtttcacg cgatggactg aaccaattct ctcgtctgcc
    37921 ggtggatgag aattcctggg atactttttc ccaaaccctg agctaccttt ccggcgacat
    37981 ctccagagca gagacctatc accaaattaa tgagcggctg agccttcttg aaggcgggga
    38041 agccaatcgc atgtactacc tggcaacacc tccagaatat tttctagaaa taatcaacaa
    38101 cctggccgcg aacggtttgc tgcaagaaga cgacgcctgg cgccgcgttg tgattgaaaa
    38161 gcctttcggc gaagacctcg aaagcgcccg ttcgctcaac cagagcattc acgcagtgat
    38221 gaaagaaaaa caggtttacc gtatcgatca ctatctcggt aaagaaaccg tgcagaatat
    38281 tttagtggcg cgtttcgcca acgcgatctt cgaaccggtg tggaatcgca actttatcga
    38341 taacgtgcaa atcaccgttg ccgaaactgt gggggtgggc acgcgtgcgc gttattatga
    38401 tcacgctggt gtgctgcgcg acatgtttca aaaccacctc atccagttgc tgacgctggt
    38461 agccatggaa ccgaccagca cctacaatgc tgacgcgctg cgcgacgaga agctgaaggt
    38521 catccgcgcc atacgcccca tgaatcgtga tgaaattatg aaaaacagcg tcatcggtca
    38581 atatcgcggt taccagcaag agcctaacgt agccccgggt tcacgaaccc ccacttacgc
    38641 cgcggtgcgt ttttatatcg ataactggcg ctggcagggc gtgccctttt acctgcgttc
    38701 aggcaagaaa atgacagaaa aagtgagcga aattctcatt cagtttaaaa aaccaccgca
    38761 catgcttttc ccgatgtcgg ggagcgattt ggaaggacgt tccaacctgc tttcgatgtg
    38821 cctgcagccc gatgaaggga ttcacctgcg ttttgaaacc aaggtacccg ataccgtggc
    38881 ggagacacgt tcggttaaca tgaattatca ctacagtgat gtgtatgacc agagcgccat
    38941 tccagaggcg tacgaacgtt tactgctgga tgtcatcacc ggtgatgctt ctcttttcac
    39001 ccgcgctgac caatctgaac tttcctgggc gctgttcgac cctatcatcg ctgggtggga
    39061 agaccctgca gcgccccagc ccgaagaata cgagcctggc agttggggcc cagacggcag
    39121 cgaacgtatg ttacgtgcag aaggccggca gtggctgcag acctgcggcg tgcattgtga
    39181 ggagaaccac aaaacttgcg atgaaaagag agttgaccct tctcgtcagc aaccctggta
    39241 gaaacaggta taattgctgc gtttcatctt caatcactgg atcattctca ttatcaaaag
    39301 ggacttgcca ttgttatgaa gcctgccctg ccctgtaaca acccgccgcg ctgctggtcc
    39361 atcccactgc tcatttttac cgctgccctc ctcctttttt taagcgcatg ccagcctgcc
    39421 ggccctgccg cgtccgagcc gattaacgcg ttgtcgcaag agaccccgcc tgcacaaaca
    39481 ccactgcttg ctacagctac acagcacgat accctctctc tctctcccac tgaaaccagt
    39541 cctcccgtcc ccacccagac caccattgtg cgaaccccgg gattctggaa agaactgcca
    39601 atcctgccgg aagagctttc agtaagagcg cgcgaaattt atcagcaggg gttggcaatg
    39661 gggaatgacc cgcgggtgta caccaagatt ggcgactgca atagcatgtc tcctgatttc
    39721 ctcgccggct ttggcggtca atacgacctg ggcgaagatt tcgcctacct ccagccggca
    39781 attgactatt ttaataaatc gtaccgcctg cccaaccagg ctaccaaccc tggcaccacc
    39841 acctccagac tgctggtttc actgtggaac aatgatgaat gcctgcccaa cgaaccgatg
    39901 cttgagtgcc agtaccgggt ggataatccc agtatcgcca tcatcatgct gggcaccatc
    39961 gacgccaaaa cgcatcagcg ccagcccgag gtttacgagc agaacctgcg ggtcattatt
    40021 gaaaaaacca tttctcttgg catcttaccg gtattggcca ccaaggcgga taacattgaa
    40081 ggcgataatt ccatcaacga gaccatcgcg cgcctggcaa tagagtatga actgcctttg
    40141 tggaacttct ggaaagcagc ccaacccctg gtaaatcacg gtttacaaac agacggagaa
    40201 catttaaatt tctggtctgc tccgccctcg accgatttct ctttgcccat cgctctggat
    40261 tacggcaagg aagtgaagaa tatcaccgcg ttgcagttgt taaatttctt aatggaagaa
    40321 ctgcctgcgc cccagtagaa atcttccagt gaaattttct ggtgaggttt tttatttcat
    40381 tattccgcac acgttcagga attccgcgcg caacgccggg tcatcggcgt acacaccgcg
    40441 gaagacagtg gtgcgggtgg caggagagat ctcgcgcacg ccgcgcatct ccacgcacat
    40501 atgcttggcg cgcatgaaca ccgccacgcc atgcggtcgc atcagcgctt ccagcgtatc
    40561 cgcgatctgg tagccaatgc gctcctgcac ggcaaagcgc ttggtgtaaa gccgtactag
    40621 gcgcgtcagt tttgatatgc ctaaaatgcg ttcatcagcc acgtagccca cgtaagcctc
    40681 gccgtaaaat ggaaaaacgt gatgctcaca caacgagtgg aagtgaatcg gtccctcgat
    40741 gacctggctc agacggcagt caggttctcc gcggcattcc cgcggaaagg acttgatcag
    40801 cttcgggtcg ccctcgtaac cgctggtcga ttcgatcaac gcctctagaa accggcgggg
    40861 ggtttcatgg gtggaggggg tgttcacgtc catgcccagc gcgctgaaaa tttcggtcat
    40921 gtacttttcg tactttttaa gctcttcagc actgatcttg cgctggttca attgcagcag
    40981 ttcatccacg gaaaaatcat caacataagt gtcagtcatg gcctctctcg tttcttttag
    41041 acttcgattt gttccccttt tctgttgtat tttatctgat attcacccgc tttgagaagg
    41101 gcaaagtgga ccgcctgtcg aggttgattc tttccagcgc ctgttgtgaa accaaacgtt
    41161 tggatcagcg tattctgaag gagagcaaac gatgaagcaa ataccagaaa atgaaatttc
    41221 gattcgctat gttcactctt ccggtcccgg aggtcagcat gtgaacaaag tcgcgacgac
    41281 cgcgcaatta cgcttcgata tcaagaattc tcttgtcctg tccgaacatg tcaaagagcg
    41341 gctgtttcgg ctggctggca ggcgcgtaac cggtgatggc gtgctggtga tcgaagccgg
    41401 tcgcttccgt gaacgcgaac gtaatcgcca ggaagcgatc aaccgtcttt ttcgcctggt
    41461 cgaacaggcg caacaaaccc cgcaaaaccg cattcctacc aggccgcgtg ccagcgacat
    41521 cacgaaacgc cttgaagcca agaaaaggcg ggcgttaatt aaattcctgc gtcatttcag
    41581 gccggaaaga gaagaataac gcccaaaaaa cggctttgtc aggttgtttt aaggttgagt
    41641 tgctcaaaaa taaactataa tgatgtagtc gatcatacgt ttaattacac tttacaagga
    41701 gaagagatat gtggtactac acgctcaaca atcaacaggt cggccccgtg gaagaagcgg
    41761 agatcaagaa actggttgaa gcgggcacaa tcacacacgg caccctcgtg tggaccaacg
    41821 gcatggccaa ctggctacca atcggacaga cccccctcgc agggctttta ggttctgtac
    41881 cacctcctgt ggctccgcca ccaatggcgg ttgtgtatga agaccctgaa gttgcctcga
    41941 tgaaaaagct cttcatgtgg ttctggatca gcctgattgg catactgctg tctgggattg
    42001 gactcatccc tgccggggta ctgttcttcg tcattttcta caaagcctgg aaacaaatcg
    42061 agcatgaggg tatccgcgct aacgccgacc aggcaacctc gcggtgcttt atccccggct
    42121 ggaattatta ctggttgttt gtcgccttcc gcggcctcgc caaagaacag ggcgataagc
    42181 ttgacagcct gaacgtttct ctggaaaaga tcgacccgaa actacccacc tggatgctga
    42241 tctgcctgtt ggggagcgcg gtcacgtttg gtgtatctct gatcgccttc ctcgtccttt
    42301 ggattatgta caccaataaa gtcaagaaca attacctcgc cattcaccag gcaagcaagt
    42361 aacttaattc aatagataaa aaaagtcccg cctccacatg gcgggacttt tttgtgatgt
    42421 agaagtattt acgcccctgc gattagaccg gcggcagcga ggttaaaaag gtaaccaggg
    42481 acgaaagcca ggccaacaac caggatggcg agaatcgtag cgatcaaact ggtcaggcgc
    42541 cctttgtgcg ccagggcttc accaggctgg aagtacattt tcaccaccac gcgcaggtaa
    42601 tagaacgccg agaccagcga agtgagcaac ccggcgaccg ccaaataggt aaagccgcct
    42661 tcaatggcgg tgcggaacag gtagaatttg ccccagaacc ccaaggtgag cgggattcca
    42721 gtgaacgaga gcatgaacac aaccatcgct gcagccaggg aaggcgcggt cttccccaac
    42781 cccgccaggt cactgatctc attcccgctg ccatcttgtt gctctaccgc caccagcacc
    42841 gaccaggcgc caagtgaagt gagcgcgtac gcagccaggt aaaacagcgc ggaagcgata
    42901 gaattctggc gtactgccgc atctccaaag cctacaaaag ccatcaggat gtaaccggcg
    42961 tgcgcaatgc tggaataagc cagcatgcgt ttgagattgg tctgcgccac cgcgaccacg
    43021 ttgccaacga tcatcgtcaa tgccgataac acccagaaca gcggcgttaa ctcaatggca
    43081 atggcggtga aattgagcga aaagacgcgt atgagtgcag cgaaccctgc agccttagcg
    43141 ccaactgaca taaaagccgt aacggaagaa ggcgaaccgt ggtacacatc gggcgtccag
    43201 ctatgaaaag gcaccgcggc caccttgaac ccgaaaccag ccagtaacag cgcgctaccg
    43261 caaatcaaca agaactgctg caccttaccc tggctgacgg cttcggtgat gccgtacagg
    43321 ctggtcgttc cagccgcgcc gtaaacaaaa gcaatcccaa agagcacaaa agcggaagcc
    43381 attgttccta ataaaaagta cttcagcgat gcttcccgtg aagcgtcgcg tttatggtca
    43441 atacctgcca acacataaag aggaatggaa agcaattcca gcgagaggaa aacgataatc
    43501 aggtcgcctg cgctgcccat cagcatcatg ccgctgatac tgaaaagcag caggacgtaa
    43561 tattcacctt cgctgatgcc aacacgcttg aggtaatcgt accccaatcc aatcgccaca
    43621 atgccgctga gggcgaacaa gttattgagg aaaatgctga aaccgtcaac acggatcatg
    43681 ccgttaaagg ctgaagcctg ttgtccaatt tggtacaggc tcgccgctaa agacagcacc
    43741 agtccagcta cagccataac aggtgtaagc aggggaacgc gttttccaaa ccacacatcc
    43801 agcagcagca tgaccaatgc ccagaccacc agcaggatga ccggaagtat ggagttgaaa
    43861 tcaattaaag tcatattttc accttccgtt aatgcgcagt ctgaacaaca gtctgaatca
    43921 gcgccagcaa ctgctcaacc gctggcgaca tgagcctgaa gaacggggcc ggatacaacc
    43981 ctatccagaa agccaacaac aggagcggcg ccagaaggat gacctcgcgc gggctgagat
    44041 cggccaattt ttcgttttcc ggatggcgta ccggtcccaa aaatacctgc tggaacatat
    44101 gcagcaggta caccgctgcc aggataacac caagcgtggc taccacggca aaccatggcg
    44161 aagccaggat agttgaatga aaagcgccca tcagaatggt aaattctccc acaaaaccat
    44221 tcagtccagg caaccctacc gaggagagga cggtaaccag tgtgagactg cccaacagcg
    44281 gagtgacgcg ccacaacccg ccaaaatcag ccatcaggcg tgtgtgccgc ctttcataca
    44341 gcatccctac aatgaggaac agcgcgccgg tggaaagccc atgattgacc atctgcaaaa
    44401 tggctccctg cacgccctgc gctgtgaggg caaatgtgcc cagtacaaca aaaccaaggt
    44461 ggctgatgga agaataggca accaaccgtt ttacgtcctt ctgcctgaaa gccacaatcg
    44521 cgccatagag gatgccgatt accgccagcc cagccatcca cggagccagt gtgagcgtcg
    44581 cctgtgggaa taacggcagg ttgaaacgca ggaacccata agcacccatc ttgagcagca
    44641 cacccgccag gatgacagac ccggcggtag gggcttcagt gtgggcgtct ggcagccagg
    44701 aatgcagcgg ccacatgggc actttgatgg caaacgccag cccgaacgcc ccaaacaacc
    44761 atagttgtgc ggtgaatgga atcgccgagt ccgtcagttt tgtcaggtca aagccgccgt
    44821 aggttaaccc cacccacagg atacccgcca gcatcagcaa tgaaccggcc atggtgtaga
    44881 gcacgaactt gaccgccgcg taaacccggc gctggctgcc ccacagccca ataagaaagt
    44941 acatgggcac cagcgtaaac tcccagaaga tataaaacaa taccatatcc agggagagga
    45001 acacgcccat catgcccgtt tcaagcagca agaagaagct cataaaacca cttacgcgtt
    45061 cctccaccgc cttccaggtg gaaaggatgg caattggcgt cagtaaagcg gtcagcaaga
    45121 ccatgagaat gctgatacca tccacaccca ggtgaaagcc aaccgtgttg ccgcccaggg
    45181 tcaaccacgg caggttgatc tccatctgga tgccgccttt cgtgctgtca aactgcatca
    45241 gcagccacag tgaaagcgca aaggtgacca gcgaggtcaa aagggcgatc cagcgcgcag
    45301 tgtttttgcc tgtgcttccc aataacaata tcagcaagac gcccacaagc ggaaagaagg
    45361 tgaccagcag aaggggatta atggctgaat tcatcgcccg tcctttacct taagaataga
    45421 aacatcatga ttgccagcag accaaccacc atccacaaag cataagagcg cacatagcca
    45481 ttttccagtt ggcgtaaccc ttcggccagc gcgcgcgtac cagctgccag cccaccgccc
    45541 acacggtcaa tgatgcccag atccaccggt tcagcgatga attgggcgaa ggctttgtaa
    45601 ggattgagga tcagcgcctg gtaaaattcg tctacccacc acttgcgttc cataccggtg
    45661 tataacgcgc cgaggggctt tttcagcggg tcatccgccc cggacttctg ctctctgcgc
    45721 gtgtagatga gccaggccaa acccagccct agcaacgcca acaagaacga gagccccgcc
    45781 accagccaga tgaactctgc gggctttacc tcgccaagcg tgtgagcgag ccagtgttcc
    45841 agcgtgtgca cgcccggcag gttgagagcc ccgcccagca atgaaagcag cgccagaatg
    45901 accagtggga tggtcattac gcgcgggctt tcgctggcat gctcggctgc tgctgagagc
    45961 ggctttccga agaagaccat caccagctgc cgccccatat aaaacgcggt aaggaaagcc
    46021 gtgaggacca gcagcacaaa cacggcgttg tgcccggtgt atgctgcgcc gaggatttcg
    46081 tccttcgaga agaagcccgc aagcggcgcg atacctgaaa gggccagacc gccaatcaga
    46141 tagacccaaa aggtaagcgg cattttcttg cgcaagccgc ccatattgcg catatcctgc
    46201 ggatcgaact cagcgctgtc gtcgccaccg tgcataccgc gttccatccc ctggatgacc
    46261 gaacccgcag acaggaagag cagcccttta aaaaaggcat gggtcaccag atggaacatc
    46321 ccggcgacca tcgcccccat gcccacggcg gccaccataa agcccagctg gctgatggtg
    46381 gaataagcca gcactttctt gatatcgtat tgccccaagg cgatggtcgc ggccaggagc
    46441 gcggtgagcg cgcccaccca catcaccacg gtctgggcaa cgggaaccac actgtaaaag
    46501 gctgtgctgc gcgccaccag atacacaccg gcggtgacca tggttgccgc gtggatgagc
    46561 gcggaaaccg gcgtgggccc tgccattgca tcgggcagcc acacgaacag aggtatctgc
    46621 gctgattttc cgctcacccc cagcagcatg gccaacgtga tcaacagcat cgctcctggg
    46681 tgcgcagctg ccacgctggc ggcctggcgg aaaacctcat cgaattgcag ggtgccaaaa
    46741 taaccgaata tcaggaacag cgcgataagc agcccaaaat cgccgatgcg gttggtgatg
    46801 aaagccttct tggctgcctc ggcattgccg atgccgttct ttcctttctc gtaccaaaaa
    46861 ccaatgagca ggtaagagca caaccccact ccctcccagc ccacgaacat cattaaaaag
    46921 ttatccgcgc tgaccaggag catcatcgaa gcgataaaca gattcatgaa gacaaagaag
    46981 cgtgtatacc tgccaggatc cccattgtgg cgcacgtcgc tgtgcatata gccgatggaa
    47041 tagatatgga tcaaggtgcc gaccaacgat accgccagca tcattaccac cgagagtgtg
    47101 tccacgcgaa aagcccagcg taccgtcaac tctccaatgg aaatccattc ccacgggctc
    47161 actactctgg cgtttggcgc agaagccagt gccgctgcta acagtacagc caccacaaag
    47221 gtaagcccag aagccaggct ggcaacgacc ccgctgaaaa cctctcccag acgcctcccc
    47281 agcagcaaat tgactaccag gccaattacc ggcaggaaga caacccaggg aacaagattg
    47341 aagatttgtc cagattccat aaaatattct cccttttgcg ggtcggtcag cctttcaggc
    47401 tgtgcatctc atcgatgctg atggtcttct tggcgcggaa aatattcacg attaatgcca
    47461 gccccacagc cacctcggcg gcggccgtga ccatgataaa aatgatgaac acctgtccat
    47521 ccaggttttg atggtagttt gcgaaggtga taaacaccag gttggcggcg ttgaacatca
    47581 actccagtga cataaggatg acaatcgcgt ttttacgcag caaaacgcca gcaaccccca
    47641 ggctgaagag gatgaccgcg aggataatgg gataaatcac cgggaacatt aggcgcctcc
    47701 atccttcttt tgcttatgtg tcagtaccac ggcaccgatg gcagctacca gcaggatgac
    47761 cgaggtaact tcaaaaggca gcgagtgttt ttggaacaac accataccca ggttctttgg
    47821 atctacaaat tcagggctag ccgcaagagt ggcgtactgg aacccatagc gtgtgatcag
    47881 cgcgtaagcg ccctggatga tcaaggcgag ggtcaacaca attgccagcg ggcgctgcca
    47941 tttgagcgtt tctcgcggat caagctgttg agtgcccagc agcatgatga caaaaagcac
    48001 cagcaccata atcgccccgg catatacggc aatctgcgaa agggcgacga aggacgcgcc
    48061 catgaggatg taaataaccg cgacggcgag aaaattcagg atcaggaata acgcggaata
    48121 aaccgtgttg cggctgagca gcatggccag ggctgaagcc accgcaatga tcccgagcaa
    48181 gataaaaatc agcaaagcag gcgtcatccc ttcctcctca atctgtgggg tctttcatct
    48241 caggcaccga acgcgtgtac aagccgggtt cagttttctg cggtgtgggc tgtccgccat
    48301 ccggtacttt caccagtaac atgtctttgg tgaaaatcgc gctgtggcgg tcgctataag
    48361 acatttcgta gatcttttca agcacaatgg cttccgtagg gcaggcgtct tcgcagaacc
    48421 cgcagtagat gcagcgcagc atgttaatct catacgtgct ggcataacgc tctcctggtg
    48481 agtagcgctc atcatccgtg ttttcagcgg cctccacaaa gatggcatct gacgggcagg
    48541 ctgccgcgca tagagcgcag ccaatgcatt tttcaagccc attgtcatac cgcttcagct
    48601 catgcctgcc gcggtagcgt tcgaacactg gccgccgctg ttcaggatac tgtatggtga
    48661 ccggtttttc aaagagtgat ttaaaagtag tgatcaatcc gcgcagcatc tctcaacctc
    48721 cgatcaatac gatcaccagc gcggtcacta acacgttcaa cagcgccagc ggaaagagga
    48781 tcttccagcc aaaagccatc attcggtcat agcggaagcg cggccaggtt gccctcaccc
    48841 agatgaacca gaaaagcagg aggatgacct tgatagccaa ataaatcggt cctaaccagg
    48901 ggtaagtgct cacgcccggg ccccaatagc cacccagaaa cagcgaagcc ccaatcatgg
    48961 aaaccgctat catcttgata tattccgcca tgtagaagag ggcaaacttc ataccagaat
    49021 actcggtgag aaatccagcg gtcaattcgt gctccgcttc aggcatatca aacggggtac
    49081 ggttgatttc agcattggca ccaatgagat aaatgatcgc gcccagcggc tgcatcacta
    49141 caaaccacag cggtttttgg gcagtgacaa tatcatttat attcatcgat cctgccaaca
    49201 gcaccggccc tacatacgcc agccccagcg ccagttcgta actgatcatc gaagcagtgg
    49261 cgcgcaacgc gcccatggtg gagtatttat tgttcgatga ccagcccgcg agcgcgatcc
    49321 cataaatgga gatggccgtc acggtgagca gataaagaat accaatgttg agattggcaa
    49381 cctgcagtgc aatctctctc ccaaaaaggc ggataggtgg tcccagcggg atgacaccgg
    49441 tgatgatgag agccgggatg accgtgagca ccggcgccaa aacaaaaagg atcttatcgg
    49501 ctttggctgg aattaattct tccttaaata tcagcttaat ggcgtctgcg acgggttgca
    49561 gcaaacccgc cggtccaacg cggttgggcc caatgcgaac ctgcatgcgc gccaacagcc
    49621 tacgttcgaa gagcgtgaca taggcgaaac ccactgtcaa gccgaggacc aggatgagcg
    49681 ctttcaagaa ccattcaaga aacatcgcaa atgtcatagc taccctcgcc tacgaattaa
    49741 ccgcgcttcg ctgcggtacg gtaagcttaa ccactaccgg agcagaaagc agcgccccca
    49801 tgctgcgtgt gaccagcacc accccttgcg gctgaccgat gttaatatcc acttcgcctt
    49861 cgatctccct gctatccagc ctgagcgaaa ttcgctcgcc cgcctttacc tggaagagtt
    49921 cagcgtcagc cggatgcagg ctgaacaaac tgcgcacaat gcgttgttgc aacataccgc
    49981 tggtggcaac cgtgccgccg cggtcataca gccgggtgat gggaaccgcc agcaggctgc
    50041 cgctttcaag ggtcagcgct tggggttggt aatgttcagc agatgaacga agcgcgcctg
    50101 ccctgagcgg cagggttacc cccaatcctt ccctgttctc atagctggtt ccgccgtaat
    50161 acatatgact gcgcccgagc gcgggctttt gctcgcgcac cgcccgaacc tctttcaggg
    50221 taataccttt gaagacctct acgctgtccg tcagttgttc aaacaagtca gccgtatttt
    50281 cacctttcag ttgaattccc gcctcttcag cgatagcgcg gatgatggcg taatcaggcc
    50341 gggtgcccgc cggggcggga actgccgggc tgtaatactg caggcggcgc tcccctgaca
    50401 ctgccgtgcc ttcccgttcc ataaccgcct gcgccggcag caccacatcc gccagtttgg
    50461 ctgtttcggt caggaacagg tcctggacaa tcataaagcc cgcgcgttgc aaggcgattt
    50521 tcaattctgg atcttcgctc gccgggtcag ccgcgcagat atacaccccc atcgcggcgc
    50581 tgatcgtctc ggcaaggcgc tcatccgtag tgtatccaat ctcccaggcg ccctgctcgt
    50641 tccctttctg ccagacggcg atcaacccgc tgctgggctt cccccggtgt ccgctgcgct
    50701 ccagccataa agcgagctgc tcagcgactg ctgctgaagc gctcaacccc atgccttcac
    50761 tgccaaaaat aaagaccagg ttctccgctt ctttcaccgc ctgcccgata tcaccggtgt
    50821 cggcttgcag cgtctgcagc atgtccagct cattcccgta agcatagctc agccggtggg
    50881 tggcgaattt atccagtcgg gtagtgcgag ggttaaccac aatgagcgtg gcgccgcgtt
    50941 ctgccgcctg cttcacacgc aaccaccaga tgggcgcttc ttcatgcagg tcgcaggcga
    51001 acaccaggat ggttgaaccc tgcgcgagtg tccccagatc tgacccgggg gacatacccg
    51061 ctaaagagac ggcttctccc ccactgatgg taccgtgcaa aatggctttt cctccggtta
    51121 catcagcgag ctgtttgagc gcaaacaggt cttcgttgct taaacgtccg ctggtaacca
    51181 tcgccagcct ggaaccagcg gcttttacct gctgcgccgc gtaccggatg gcttcatccc
    51241 agctggtttg taccagttcg tcaccgcgcc gaatgagcgg ggcggtcaag cgctcagggc
    51301 tttcataata atggtaagtg aacctgcctt tatcgcaaat ccatatttcg ttcacggctt
    51361 cgttctggcg cggcattacc cgtttgatca ctgtgcgccc gtctttgcgc gcctctcggc
    51421 gcacattgta ggtgatgttg cagccaaccg ggcactgggt gcagattgac gctttgtcct
    51481 tcatctccca gggacgcgcg ccaaagcgga aatcagcggt ggtgagggcg ccgaccgggc
    51541 aaatgtcggt ggtgttgcct gagaatattg agtcaaaccc ggggtctgaa taagttctga
    51601 tctccagcgc ccgtccgcgg ttataaaaac cgatcacggg gtcgtcaacg atctgctcct
    51661 ggaagcgcac acaacgcccg caatggatgc agcgttcgcg gtcgaggatg atcagttcgc
    51721 ccaacggtac ccttttctgg ttatggctct tctcatcaaa ttcgaaacgg ctttcagcag
    51781 ggccaaactt catggtcagg ttctgcagcg ggcattcgcc gcctttatcg cacaccgggc
    51841 aatccaatgg atgcgaggta aggagaaact caagcacctc tttacgggct tccatcacct
    51901 tttcagttgc gccccacacc accatgcctt ctgatacagg cgtggtgcag gcagtctcca
    51961 gcttggggcc gaatacaatt ttgggtgtgc cgtcttcatt caacacgggc gcgccgctag
    52021 cgcgatcaag ttgcggtctt cccacatcca ccaggcaaac gcggcacatt cccgcgggct
    52081 tgagcttggg atggtagcag aacaccggga tgacaattcc cagttgtttt gccgcgtcca
    52141 caatcagggt cccttcaggg acagtgacgc tgcggccgtc tatcttcagt gtgacttgtt
    52201 tttccatccg tttatttctc caggcgggcc gcgaagtcag cccggtagtt tttaatcgct
    52261 gaaattacgg atacagtgga gaactctcca agcgcgcaca ggcatttgtt ttgcatctgg
    52321 ctggcaatac tggcgagcag gtcaatatct tcttctttcg ccttgccggc ttcgattcga
    52381 tcgatcaccc tcgccatcca gaaggtgcct tcgcggcaag gagtgcattt accgcaggat
    52441 tcgtgcttga agaaatgaat cgtatcgctc accacggtgc tggcattgac cgtttcatca
    52501 aggatgatga ctgaagcgga accgagcgcg cagcccagag cgcccactga ctcgtaatcc
    52561 atgggggtat ccagcgcaac ctcagtggcg gggatgagtg aggaagaagc ccctgccggc
    52621 atgatggact taatctgcgc gtcattaatg atgccctcgc cgtgttggta gatcaattct
    52681 ctgaaggtgg tgcccagcgg caactcgtaa ttaccaggct tttttactct tccggatagg
    52741 ctgaatattt ttgtgccggg gcttttctct gttccctgtg cgcgaaagta cgccgcccct
    52801 ttgccgatga tcaacggcaa gttggtaagc gtttccacgt tgttaatcac cgtggggtag
    52861 ccgaatagcc ccgagaccgc cgggaaaggc gggcgcaacc gcggctgccc gaggcgccct
    52921 tccattgact ctaataatgc ggtttcttcc ccgcagatat aggcgcccgc gcccagatga
    52981 gtgtggatgc gcagagagta atccttgcca aagaggttgt cacccaggta gccgctgctg
    53041 gtcagttcag cgatgcgctc atccagctta tgcgcgatat gccagaactc tccgcgcagg
    53101 tacacatacg cggtgtgggc gcgcaccgcg taagatgcca acatcaggcc ctccagaaac
    53161 tgataggggt tgaattccag tatctcgcgg tctttgaatg tgccgggttc agattcatca
    53221 gcgttggcca ccacgtaatg cggccaaatg gtattgggta aaaaagacca tttagcgcct
    53281 gttggaaaac ccgccccgcc gcgcccgcgc agacccgaat ttttgacttc attcaccagg
    53341 tcttcaggcg tcaatttcgt gaccgctttt ttccaggact caaacccgcc atgctgcagg
    53401 taagtagaga gctggttcag acctggtatc tggcgatggc gcaagagcca taactcactc
    53461 atggtcgcct ccttctggtt ctggtgatag cggttcatcg ctctcaggca ggcgcaagga
    53521 agccatcatc gaacgcagcc aatccattgt gcgggcgact gtctggtgtt cgtgatattc
    53581 gagttcatct cccatttgcg attgaaacat gggcgcgcga tcgcaggctg ccaggcattt
    53641 cacctcttca acggtcacca gcccatcggg tgttgtacca ccgggcttga tatccagttc
    53701 ggcgcacaac ccatccagat attctcttga accgcgtaac gcgcaggcga tgtcggtgca
    53761 cacctggatg cggattttcc cgcctggctc agtgtgaaac agggtgtaaa aacccactac
    53821 cgaagccacc tcggtgactt ccacttcggc gatttcggca atttcagcca tggtctctcg
    53881 ggagatatag cccctgtctt gctgcgccag gtgcagcagc ggcatcacgc tggcgcgctt
    53941 ctgatctggc gggtatttcg ccaggatctt tttaatttct tgcgggaatt ttttaagcag
    54001 ttggttcacc ggtcacagtc tcccagtacg atatcgatgc tgccaatgat accgatcata
    54061 tctgccacga agtggccttt cgcgatcaca ggcaaggcat gcagcaacgg accagagggc
    54121 gtgcggtaat gtacacgata aggtttgggg gtgccgtttg attccatgaa cacgcccatt
    54181 tcaccgcgcg ggctttctac cgcggtgtaa accgcgcctt tcggggggat aaagccctcc
    54241 gtccatagtt tgaagtgatg gatcaacgcc tccatgctta ccccgatttc tgagcggggg
    54301 ggtggtacgt atttgtaatt cggatcacgc accgggccgt agggcaattt atccatcgcc
    54361 tgctcgataa tgcgcaccga ctggcgcatt tcttccatgc gcaccaggta gcgcgcgtac
    54421 acgtcgcctt cgctctgcgt gggaatgtca aaatcataca ggtcgtaccc gctgtaaggg
    54481 tttgacttgc gcacatcatg cgccagtccg ctggctcgca gcgtgggtcc tgttacatcg
    54541 taagccaggc aggtgcgggt atcaattttg ccaatgccct tggtgcggtc aaggaaaagc
    54601 gggttacgcg tgagcatgga ttcgtattca gccatgcgtt tgggcatcac cttggtaaaa
    54661 tcgcgcacca ttttttcaaa ctgcaccggt acatcacgcc acaacccgcc gggacggatg
    54721 aaagtagtca tcatacgcgc accggagacc agttcaaaaa tatccaatat cagctcgcgt
    54781 tcgcgcatgg tgtaaaggaa agtggactgg gctgccaggt ccaaggcgga ggtacccagc
    54841 cataacaggt gtgactggat acgcgaaagc tccgtcaaaa tcacacggat cgcctcggcg
    54901 cgcggcggcg gttgcagccc gatcagtttt tccaccgcca gtgagtacac cagcgtgttg
    54961 cccatggcat acatgtagtc catgcggtca acaagcactt ccgctttctg gtaggtcttg
    55021 ctctccatcg tcttctcaat gccagtgtgc aggtagccga catcgggaat gcaattgacg
    55081 ataatctcgc cgtcaagctc gaggatcagg cgcagcacac cgtgcgtact ggggtgttga
    55141 ggtcccatat tgagcaccac agtctgcccg ctgaacgctt tttcactcag atggtgcttg
    55201 agttcttcta aggtgaattc ctggatctcg cgcatgttta gagcaccccc ttgggcttgc
    55261 ggatgtcgat ttcgtcaaag ttgaaagtga actgcacttc ctcgtagccc agcgggtaat
    55321 ctttgcgtag cggatgcccc tcccaatcag gcggcatcaa caaccggcgc aggtctgaat
    55381 gcccctcgaa gcggataccg aacaggtccc atacctcgcg ttcgcgccag ttggcgctgg
    55441 ggtacaccgt ctcgagcgtc cggatggtgg gggcatcacc atccaacgga actcgaacat
    55501 tcaggtaggc atggttggcg gtggaataaa agatatacac caggtgaaag cgtggattgg
    55561 tttgcgggca gtaatccact gccgtcacca cactgagcat gtcaaaggcg aattcatcac
    55621 gcaaaaccgt ggcggcttct acattggcat ctgcagaaat aagcaaggtc acctcgccac
    55681 ggaattcgct ggtttgcgcc ttgaactgct gctggattct ttcaactgcc ggggagagaa
    55741 tctcgttcat gcggcctcta cttccagtcg gagacttttt cagtgcgaat tttttcgtac
    55801 agggtgagaa taccgtggat cagtccttca ggtcgtggcg ggcagccagc cacgtacaca
    55861 tccacgggta cgatttcatc caccccctgg taaacggcat agttgttata tactccgcca
    55921 caagaagcgc agtcacccat ggcgatgacc cacttgggat cgggcatctg gtcatacaga
    55981 ttgcgcagca ccgggcccat tttttgcgaa acgcgccccg ccacgatcat caggtctgat
    56041 tgccgggggc tggcgcgcat cagttccata ccgaagcggc tcatatcgta atttgaagcc
    56101 tgcatggcca tcatctcaat ggcgcagcag gccaagccaa aggtcatggg ccatagcgag
    56161 ccggtgcgcg cccaattgac cattttatcc agcgaggtgg ttaccactcc agaacttccg
    56221 ctcttaggct ctactcccat tccatcgctc cttttttcca cagataaaca aagccggtca
    56281 tcaggatggt cacgaagatg cccatttcaa tgatggcgaa cagcccaagt ttttgaaaag
    56341 tgaccgccca ggggagaaag aagatcactt cgacatcaaa gaggatgaac agcactgcca
    56401 ccaggtaaaa tcgcaccggc atctggcgcg taccggggcc ataagggatc ataccagatt
    56461 cgtaaggctc gcctttcttt ttcgtcgggc ggtacggtcc caggtacctc cccagcagca
    56521 gtacgatgat acccactgca atggagagag caagcagtat aacaataggt atatattcaa
    56581 aaggcatggg aacaatctcc tgttggagag aatcaatcaa cccgggatga tgctgctttc
    56641 ccagttccct ctattttacc tgaaaaatgg atatcaataa aaaaaccccc gccgtttggc
    56701 gggggtagag caaattgagt tgccctgttt acggttgtgg cgcggcttta ttcgcttgcc
    56761 ttggtctgtc cgcttccccc tttttagacg gtaccggaat cataaagcgc tattgcgcca
    56821 ggccgttggt caggctgtct caaggtgtta cctggctttc tcgtattgct gttccacaaa
    56881 actccagtta accactgacc agaaggcttt gatgtaatct gggcggcggt tctggtaatt
    56941 caaatagtag gcgtgctccc acacatccaa cccgaggatg ggaatgtgat ggtccatata
    57001 tgggctatct tgattggcag tagagtatac ctgcaattgt ttctgaccgt cgagcaccag
    57061 ccaggcccaa ccagagccga agcgggtggt cgcagcagca gtgaactttt ccataaaggc
    57121 atcggtagag ccgaaagcgc tttcaatggc ttttagcagc gcgccggagg gtgcgctttt
    57181 tccacccggg ttgagcacgt cccaaaacag ggagtgattg gcatgacctc cgccgttatt
    57241 acgcaccgcg gtgcggatgg tttcggggac ggtattgatg ccttttaaaa tgtcttcgat
    57301 tggcatggag aaaaattcag gcgaagcttc taatgccttg ttaaggttgg cgacataggc
    57361 ggcatggtgt ttgccgtggt gaatctccat cgtgcgcgcg tcaatggctg gttccagcgc
    57421 gtcgaaggcg aatttgagtt gcggtagttc aaaaaccttg ttcatgattt cccttcccat
    57481 tgttattgcg tactattaag ataataataa tcatatttac aacaaaagtc aagaggtttt
    57541 tataagaaga atattagaaa tgggattcac ttggctgatc ggttaaaaag aaaacacgtt
    57601 gaaggagttg ctccctttca ccacaaccta tttagcgcct gtggtttccc gaaccaaccg
    57661 ttcttttcgg ctctctaatg cttgacaagc cttctccgca actatactat aaagaagatc
    57721 tggtttagct gaactcatcc gttatttaag aagaaatcct gcctgcggag ggtacccatg
    57781 aaacgcctgc tgaaatggta cgaacttctc ttcctcaata gctatcagtt ggggttgagt
    57841 atcgcttcca acgtcatcag cccgctgctg ctgccagcgc tggtgctggt ctttatgcct
    57901 ccggaaaaga agaatacctc gaccgcgctg gtatacgtca ccggtctggc ggttgccatg
    57961 ttcattcaac ccgtgatggg tatgctcagc gaccgttcca cttcaaaaat ggggcggcgt
    58021 aggcctttca tcatcgtggg tgcgttgttg aacattcttt ttttaggcgt gatgggcgct
    58081 tctttgtggt ttaaaagctc acctctcaac gctttcttac aatccagttt cggcatcacg
    58141 gcttccctgg cagtgctatt actgggcatt attctgctgc agttttcttc caacatcagc
    58201 caggccgggc agcaggggtt gatacccgac attgttccag aacagcagcg cggtcgcgcc
    58261 tcgggcatta aatcagtgat ggaaatggtt cctgccgcgc tggtgttgct catctcgccg
    58321 ctcattgatc gacagaaatt ctggctggtc attctcatcc ttatgctttc cttcctggtc
    58381 accatgctca tcacggtagc aggcacgcgc gaagaacctc tccaagaaaa acctccgcgc
    58441 gccgaagacc ggcctgtcct gcggatgctg ctgctgaccg tcatcttcgt gggcatcacc
    58501 cagctggctc ttctgctggt taaaggcatc ggcacctggc tgccgcagga cagtaccagc
    58561 ctggcgctgc gtatcgtcct ggtcggtttg gcggggcttg tgggtatggc aggctccatc
    58621 ttcatcggcg tttacttcgg cgcgcgggtg ggcatcggca aagaagccaa cacccataaa
    58681 tccttcatct ggtgggtggt cacgcgcctg atgttccttg cagccatcag ctcagtgcga
    58741 aatttcgcca tgttttttgt aaaggatgtg ctgcaggtgc ccaacccggc caccattacc
    58801 acctacctga cggcggtgat tttcattttc ctgctcattt cctccattgt cggcggcatc
    58861 ctggcggaca aaatcgggcg caagaagttg ctggctgccg ccggcgcgct ggcggtgctg
    58921 ggcaccatca tcctggtctt ttcgcgtacc attcccatgg tgatgtttgc cggcgttttc
    58981 ctgggtttgg gcaccggcac tttcatggct tccaactggg cgctgggtac tgacctggtg
    59041 ccacagaaag acgctgggcg ctacctgggt atttccaacc tggccggggc gggcgcgggc
    59101 ataattggct ccagtatcgg cggtccgctg gcggactact tcaacgccat ccagccaggg
    59161 ctgggttatc ccgttatttt tgccctctac ggcgggttgt tcctgctttc cgtgctctta
    59221 ctaccgcgca tccaagccga gacttcgttg agctgaatcg atggagcgca gcgttcacct
    59281 gatctttaaa actcatttgg atgtgggttt taccgattac gccgcggtgg tggtgcggaa
    59341 ttatttcaag aaatatatcc cggcggcgct gcgcctggcg cgcgaactgc gccaatcagg
    59401 ctcagacagg ttcatctgga ccaccggttc ctggcttatc tacgagtatc tcgaacaggc
    59461 ttcgtccgcg gaacgcgggc agatggaaga ggctattctg gcgggggata tcgcctggca
    59521 cgcgctaccc ttcaccactc acaccgagct gatggacgcc gagcttttcc gctttggcct
    59581 gtcgctttcg cgcacgctgg atcaacgctt tgggcgccat accatcgccg ccaaatggac
    59641 cgatgtgccg ggtcatacac gggcgatcat tccgctgctg gcagaagcag gtgtggtatt
    59701 tttacacatc ggtgtgaatc cgggctccac cgtgccctcc gtgccaagcc tgttccgctg
    59761 gcaagaccct ggcggcgcgg agttgctggt aatgtacgag agcggttacg gctccgtttt
    59821 cgaaataccc gggttggagc actccctggc gtttggtcat accatggata acatgggccc
    59881 gcaatctcac gagcaggtgc tggatgtgta tcgagaggtg cgctcccgcc agccaggagc
    59941 gcgtgtgttc gcttccacac tcagtgattt tgccgcgcag ttggtgcgag tgcgcgaaaa
    60001 cctgcccgtg ctatcgcagg agattggtga tacctggatc cacggtgttg gcagcgaccc
    60061 catcaaggtt gcgcgttttc gcgagttgct gcgcttacgg gctgaatggc tcgccagcgg
    60121 tcgagtaacg cggagtgaac catccctcat taacttaaac cgctggctgc tcatggtacc
    60181 cgaacacacc tgggggatgg atgaaaagac ctggctgaat gaccatgaaa actacactgt
    60241 gaaagatttc aacctcgcgc gcgggcagcc gaactttcag cgcttcgaag cctcctgggt
    60301 tgagaagcgc gcttacccgc aactggcagt caacgcgtta ggaggtggct cgctggcaga
    60361 tgaagcccgc gcccgcctgg aatccattcg cccgcgagtg ccagacctga gcgcctggca
    60421 gccaatcgaa cccgcccagg caaagctgga aaacccatcc ctcgctgtga catttgaaca
    60481 acagaaaggc agcctcgacc agctggtgga tcgcacaacc gggcaaagcc tggttgtgcg
    60541 cccaggtgag ttcagtgtgt tctcatatca gaccttttcc agcgaggatt atgagcgttt
    60601 cttcaacacc tatgtattgc cgcagcagcg ccacggctgg gcgctggaag atttctgtaa
    60661 acccggcctg cgccccgaag acagccccag ccgctgctgg ttgccgctgg taaaaggaat
    60721 attcaagcgc tccacgcctg gggttgaaca cattctcttt tatctcacct ttgaagacga
    60781 ggctttgcac caattcggcg cgccgcgcaa tggttatctg cagtactctc taccgtcgca
    60841 ggagcagcgt ttgcacatcg acctgcaatg gttcaacaaa caagcctcgc gtatgcccga
    60901 agccctgtgg atgactttta gaccggagca tcaccctgac gccgagtggt cgatcgagaa
    60961 actggggcgg gagattccag cgctgagcgt ggtggaaaac ggcaaccgcc acctgcatgg
    61021 tagcgggcgc tatgtgacat gccgcgccct aggcagcgaa ttgcgcatct ccgccctcga
    61081 ttctccgctg gtggcgttgg gcgcccgcag cctgctggat ttcaataatg accagccgga
    61141 tacccgccag ggcgcgcact tctgcctgtt caacaacctg tggggcacca actttcctat
    61201 gtggttcgaa gaagactgcc gctttagatt tgacatcacc cttaacccaa ccaaggagga
    61261 gcgaaatgcg tgaattaagc ctgcccggta gggcgaagcc ttatgtaatg gctcaccgcg
    61321 gcaaccgcgt gctgtgcccg gagaacaccc tatctgcctt tctgaaggca ttcgaagaag
    61381 gcgctgacat cctcgagact gacctgcacc tcagcgccga cggcgtgttc atgtgtattc
    61441 atgacgccac ggtagaccgt accaccaacg gtaaaggcgc ggtgaattct ctcaccctgg
    61501 atgagctcaa gcagttgagc gcgtcttaca acttcaaagg ctttgaaaac gagcgtattc
    61561 ccaccctggc agaaaccgcg ggaatcttgc cgccgggggt ggcgctggcg ctggagctca
    61621 agacagaccg cttcctcgaa gaagaggtgt gccgccgctt ggtcgctgaa ctgcgctcca
    61681 gcggtattct cgagcgcagc ctgatgcttt ccttctcgct gccgcgcctg caggcggtga
    61741 aggctgttga cccttccatt cccattggtt ggatcaccat gtcccagttg acccccaacc
    61801 ccctgcccga ggtgctcggt ccgttctggc caggagtctt cctcaacccc ttctttgtcc
    61861 gccgcgccca ccagcgcgga caactggttt gcccgctgga ccccacgccc gacaggcgcc
    61921 tgtggtatta caaactgctc aactgtgacg cgctggtaac agacaacccg ggcgcaaccc
    61981 tcgaggtatt aaagcgtata tcctttaagt aaacctttca ctgcgaggtg cagccatgtc
    62041 tgtgaatcaa aagcgagttt ctttgttctt accggtgatc ctgattggcg cgggtgttat
    62101 tcttttactg cgcaaccttg gttttctacc tgcgttcaac tggagtgttt tgctcaggct
    62161 ctggccgctg gcgctggtag tgttagggct tgacctgctt tttggctacc gggccccctg
    62221 ggcgggcgga ctgatcggtt tgctcacagt cgcggcggta gccgttttcc tcttcttcag
    62281 ccctgtaatg gagaataacc cgccggcggg cattaagact gaagtggttt cgaaaccgct
    62341 agagaacaca gcgcacgtgc aatattacct ggatacctct tatccgccag tgaccatcag
    62401 cgccttgccc gattccaccg acctggtcaa ggcgaccatt gtgcaccgcg gtcaggtgca
    62461 ttttgacgtt aaaggtgata cagataagac cgttaccctg agcacaagca ctgagcctgg
    62521 cccctggttc tcgtatgatc tggggttggg cggacaaaag tgggatattg ggttgaaccc
    62581 cagcgttcct accaccctta atctcgatgg cggctccggc gcgctcgatc tgcaactgga
    62641 aggcatcacc cttgaggcgc tgcgagcgga tttcggttct ggggcgtcca aaatcaccct
    62701 gccggattcg ccgcaaccct accaggtgga tattgaaagt ggttcaggtt cggtcgaact
    62761 cgctctgcca acagataccg atgtgacctt cacgctcgaa agcggcagcg gggcagtaac
    62821 cgtttttctg ccgttgactt cagcggtgaa aatcgaggtt atggacaatg gttcaggcac
    62881 ggtgcacgtc ccagccggct atatcgccgc tgcaggcaac acagccgacc atgggacctg
    62941 gcaaagcagc aatttttcct ccgccgccca caccatcctc atccgcgtga aagaccgcgg
    63001 atcgggcaat cttttcttta aaacaaggta attcaacgca acccatcatt tttttcaaga
    63061 atcccgccgt gacgggattc tgttttgtgg taaactctta ttgaaaaacg ttttcaataa
    63121 gaggaacctt gaacaagctc gaacactggt tgaaaaccct gaaagaagat ggctgccgca
    63181 tcacctcccc gcggcgggtt atccttgaag tgctcatcga cagtccccgt gctttacagc
    63241 cagcggaggt gcacgctctg tgcaggcaaa cccaccccgg cattggactg gtgaccgttt
    63301 atcgcacact ggaacgcctg gaatcgctgg ggttgattca acgcgtgcac cgcgaagacg
    63361 gctgccacct tttaatgccc gctgcagacg ggcatgaaca ttatcttgtt tgtaccacct
    63421 gcaggcgcac cattctcttt aaaggagaca acctggcgcc cttgttcacc aaggtggaat
    63481 cacgcaccgg ttaccaggtg agtgagcact ggctgcagct atttggtacc tgccctgact
    63541 gccagttgaa tagccagcag gttacaccga aggtcaacca tgcataaaaa aaaatcgctc
    63601 ctgctcttgc tctcgtttat catcctgctg gcgagtgcct gcactggcca gatcacacca
    63661 acagccaaac agctgccggt tgtggcaacc acttccatcc tggcagatgt tgtcagccgg
    63721 gtgggcggta accttgtttc cgtcaccacc cttgtccccc ctggcgtaaa cgagcatgaa
    63781 taccagccct cgccgcgcga catcgctgcg gtatctgacg ctgccttggt ttttgaagtt
    63841 ggactgggat tggagcaatt tatggacgcc attatccgta acgcgtccga cgatattacg
    63901 cccattgagg tatccagtgg catctccacc caggagttgt taatcccgca tgatgcaaac
    63961 accgcggaac agcagcacga acacacaacc gacccgcatg tgtggctgga ccccgccaac
    64021 gtgatcgttt ggacggagaa catcgctgcc gcgttgagcg cgcgtgaccc agccggcagc
    64081 ggcacctacc aggcgaatgc cgcggcttac atcgctgaat taaaagcatt ggacgagtgg
    64141 attagaagtg agaccgcgcg cattccacag gaaaaacgcc tgatcgtcac ggaccacatg
    64201 ctttttaatt acttcgctga aaaatacggt ttcactgtgg tcggtgcgct catcccttcc
    64261 tatagctctc tggcccaacc ctccgcgcag gaaatcgccg cgcttgaaga cgccatccgc
    64321 cagtacaacg tgcgggtgat actggtaggt aacacagtca accccacatt ggccgcgcgt
    64381 gttgccgaag atacgggcac ggcaatggtg cagttttaca ccggttccct ctccaacgcc
    64441 gatggaccgg ctgctactta cctggattac atgcgctaca acgtgaagac cattgtcaac
    64501 gccctgagtg gaaaatagta cacatgaatt cggttgccca acccactgca aacaccatca
    64561 caccgcgcag tcacccggtg catattcccg gtgaacccat tttgcgggtg gagcacctca
    64621 gcctggctta tgaaggcgtg gtggcgctgc agaatgtgac ctttcagctc atcaccggtg
    64681 aacgtatggc ggtcatcggc cccaacggcg ccggtaagag caccttgttt aaaatcatcg
    64741 cgggcttgtt gaaacctgga agcggcacag tgagcgtgtt cggagccgag cccagcggtc
    64801 atatctgcat ttcctacctt ccgcagcaca accacgtgga ctggaatttc ccggtaaccg
    64861 tgggcgaagt ggtaatgatg gggcgcaccc ggaaaatcgg cttcttccgc tggccttctg
    64921 ccggcgatcg cgagatcgtc agccgcgccc tgcgcacggt tgggcttcac gctcatgccc
    64981 accgtcagat cagccaactt tcgggcgggc agcagcaacg catgttcatc gcccgcgccc
    65041 tggcgcagga agccgacctg atgttgatgg acgagcccgt gaccgggctg gacacgcctt
    65101 cgcaggaaga acttttcact gttctggata ctttaagaga agagaaggtg acggtgatgt
    65161 tcgcgctgca tgaccttaaa ctggctgcca cctgctttga ccgcgtcatg ctcctcaacc
    65221 gccgcctggt gggtattggc aaacccgccg atgtatttac cagagagcat ctcagcaagg
    65281 cgtatggtga ccgtgctcag gtgctggaga acggtcatgg tctatccatg gtggatgaca
    65341 cctgctgcgg cggaagggaa gaatgataga ctggttatgg accccgctgc agtacgcctt
    65401 tatggttcgc gggatgctgg cggccgtgat tgtgggcatg gtgtgcgcgg tgttgggtac
    65461 ctacgtggtg ctgcgtggaa tggccttctt tggcgatgcc ctggcgcacg cagttctccc
    65521 cggggtggcg gtaggttacc tcctgggcgg cggcgcgaga gggccggtat tttggtgggc
    65581 catggccacc gccctgctca gcgcttttgg tatcggcgcg gtcagccgcg ccgcccgcat
    65641 taaagaggat acttcaatcg gtatcatctt cgccgggatg tttgccctcg gggtcgcgct
    65701 catttcaatg gtacgcaatt acaccgctga cctgacgcac tttctttttg gagacgtgct
    65761 tggcgtccgc gacactgacc tgctgctcac cgctgtattc ggcgcgctga taatgctctt
    65821 catcctgacc ttttataaag aattcctggt catctccttt gacccaacac tggcagccac
    65881 gctgcgcctg cctattcgtt ggctcgatta cctgctcatt gccctgatcg ccatgagcat
    65941 cgtggtatct ctgcagacgg taggcgttac cctgatggtg gcgatgctca tcaccccggc
    66001 ggccgcggct tcgctgctga cgcgccggct ttcccgcatg atgttgattg ccgcgcttat
    66061 cgcgattttc tccggtatca gcgggttgta cctttcgtat tacctcaacc tggcttccgg
    66121 ttctgccatc gtgctgacct gtacagcggt ttttgcaatt atttggggca ttaccgctct
    66181 cgccaggcgc tactcacgcg cgcgagctac tgaaaacatg tgatacaatt ttttcccaca
    66241 ccactataga aaggtagagc atgagcgatc aaaccaaaca atggtctact cctcccgaaa
    66301 tgagcataga ccctgagaaa tcctacaccg cgaccttcaa gacagacctg ggcagcatca
    66361 tatgcagcct gcacgccaaa aaaaccccgc gcacggtcaa taactttgtc ttcctggcgc
    66421 gcgagggttt ttacgacaac accatctttc accgggtcat ccctgacttc atggctcagg
    66481 gaggggatcc cacaggaacc ggtatgggcg gacctggtta caagttcaaa gatgagttcg
    66541 cctcctcgct tcgtcataat gccccgggaa tactttccat ggccaacgcc ggcccgaaca
    66601 ccaacggctc gcaatttttt attacccacg tgcccacgcc ctggctggac aataagcatt
    66661 ctgtctttgg ccaggtgatc aagggattgg acgtgctgct ttccatcccg gcgcgcgacc
    66721 cctcccagcg caacagcccg gctgtgaagc tgctgacggt ggaaattgcc gaagcctgaa
    66781 ccggtcagcg gcctggcccc tggtgaatcg taacggtaaa cagggagacc gcggagtttg
    66841 catatttgtt aagtcatttg tgcaaatgag tgatatactg cttctatcaa atgcttgggt
    66901 gccccctcac ccaggcatta ggtgctttta aagggcaggt gaccagccct tttgagatca
    66961 gcctgatgag ttggcgtttt ccttgacgtt tctggtgggt tggggtatat ttcaggcgct
    67021 caatatcgca tgcccccgta gcgcaattgg atagagcgtt tgcttgcgga gcaaaaggtt
    67081 gagtattcga attactccgg gggcacctaa aaaagagcgg cacaccgctc ttttttctat
    67141 agaaaacagg atcactggat tcgctttatg aaaaaaccac agatactcct tacaaacgat
    67201 gacggcatcc attctcccgg gttgtgggcc gctgcccgta aactggcaaa actcggttac
    67261 gtaacagtgg ccgcgccacg cgaacagcat tcaggcgcag gtcgcagcgt gcccgcctgg
    67321 actgacggag aagtgaaacc caccacgctt cagattggtg accaagaatg gacctgttac
    67381 gctgtgggcg gttctcccgc tcagtcggtg cagtatgggg tcttcgccat actgcagcaa
    67441 aaacccgacc tggtggtatc gggcattaat tatggcgaga acccagccac agatgtctct
    67501 ctttcgggca cggtcggagc ggggttggaa gccgcttcct acggcattcc ggcgctggca
    67561 gtctccctgg agctggaaaa cgaagattac ctggggtatt cgcgcgcgac tgatttctcc
    67621 accgccgcgg tgttcacccg ccgttttgcc aaactgctgc tcgctcgcca gatgcctgaa
    67681 gacgtaatgg tattaaatgt taacgtgccc gtgagcgcca cgccagatac cccctggcgg
    67741 gttacccggc tgggaaaaca ggcctacttc gtgccatttc tcaagcaacc tgaaaaagca
    67801 ggcgccattc ctcgtattga tgcttcacgc aacccccatc cctccgacct gggtgacccc
    67861 accacggatg tgcatgtgct cctgcgccag aaaatggttt ctgtcacgcc gctcagtctt
    67921 gacctgactt cacgcgtcga cctggaggaa cttcagcgtt cgttcggcac gctggattga
    67981 agaaaaaaca acctcgccag gaggttgttt ttgtatcgtt gcgcttcagc gttttgtgac
    68041 attgaagaga atattgagca cgtccccgtc tttgaccacg tactccttgc cctccaggcg
    68101 caattttccg tgggctttcg cttcgttcat ccctcccagc gctaccaggt catcatagtg
    68161 caccacttcg gcgcgaatga accccttttg taagtcagtg tggatctcgc cggccgcttc
    68221 aggcgcggtg gcgccgcgtt ttaccgtcca ggcgcggcat tcatccggac caacagtgaa
    68281 aaaagaatgt aatcccagca ggtcataaga caggcggatc atgcggctga gactgggctc
    68341 ttcaataccg tattccgcca ggaatagcgc cgcttcgtcg gctgaaagct gggcgatgtc
    68401 catctccagt ttcccctgca ggctgatcac cctgctgttc tgatgggtgt agtcaaccac
    68461 aggctgcgcc tgtccttccg ccaggttaag cacgatcatc atcggttttc ggcttaagaa
    68521 gccaaaccca gagagcagct tttcgtcttc ttcgctgatc tccacatcgc gcagcggcac
    68581 ttcacctgcc aggatttcct gcaaacgggt gaagagcgcg tgttcgcgct cgatcagacc
    68641 tttctctctg ccggcgcctt tctttttttc ttcttccagg cgctccagct tgcgttccac
    68701 cgcgatgagg tcgttgagca caaattcact atccatatta aggatgtcgc gctgcgggtt
    68761 gatgctctcg tgcacgtgag ccacattttc gtcagtaaag gcgcgcacca cctggataaa
    68821 accgtccatt tgtgtcagtt gattgagcaa agcgccagag atgccgcttt tgctcgcgct
    68881 gccgtccaat ccagcgatat ccacatatgt gaccttggcg tagatggttt ttttcggctt
    68941 gaacatggct gaaagtacgt ccacgcgcct atcgggcaca tccaccaccg cggtatggat
    69001 ttcaattttc ccgctggcac cggtcggctg cgtggcgcgg gtgagcgcgt tgaataatgt
    69061 cgtttttcct gattgaggta atccgatgat tcctagacgc atggtcgttt cccttgaccc
    69121 ttgtgaatta gtcagatata ctctgttcgg tatcgccgga gtggcggaac tggcagacgc
    69181 gcacgactca aaatcgtgtt ccctcgggag tgtgggttcg attcccacct tcggcacaag
    69241 gattataaca ttttctcccc gctcaagagc gggttttttc ttccaaaaat ccacctggtt
    69301 ggatttgttt ctagaaataa ttacagcgct aagcgcaaaa tcggtcggtt ttctcgcatc
    69361 gccacctgct taaaccccag gcggcgaaat acagatgctt taccggtaaa gaggtggact
    69421 gggctcactt ttccttccgc ctccactgga aatgcctcaa ggatcttaac gcccttgctt
    69481 ttcgcgtaat cgatggcagc catgagtaat ttttccattc ccccctggca gcggtaatcg
    69541 cggtggatga aaaaacaggg cagcacccaa accggctggt cgtcgaccgg gcccaatacc
    69601 gttgaggtcg ccaggcgttt gtaactttca cgcggtccca gcgccaccca accagcgggc
    69661 acaccatcgg cgtaagccac caggccgggt tccacgccag agtgaacgat cgttcgcatc
    69721 aactctttgt gaccttcttt cttcgacgcg ctgaattccc ggctggtcat gcggaaccag
    69781 gcgcaccagc agccggcgca ggcgccattg ggcccaaaga gaagctcaaa atcctcccaa
    69841 cgggcgggag tcagcggggt atactcccat ttttgcaccg caaccggttt ttttgccata
    69901 acggctgcct ttctcaccaa aagggttgat cggaatattg ttctttctga ttatacaact
    69961 tctactccag gtttaaatac ttattggttg gcgttttttt tttactggaa tttttacttt
    70021 atacgttgta tactcaagca tacaaaacta gcgaaaagag ggcgctcatg aaggttactg
    70081 tcacccaaca acacctggcg cacggcttaa gcatcgtcag ccgtgcagta tctccgcgta
    70141 cgaccctgcc tgtgctggca aatgtactgt tggccaccga cgagggacgc ctgcgccttt
    70201 ctgccaccaa ccttgagctg ggcatcacct gctggatacc tgctcagata gaggaggaag
    70261 gctccattac ccttccttcc cgtaccctgg tggacctggt cagcaccttt cccccggata
    70321 ccgtccatct gacgctgaac gatcgcaccc aaaccctcaa cctggtgtgc aacagctcag
    70381 tgcacgatat caagggcatc gacgcgcagg acttcccgcc catgccagtg accgacccgg
    70441 aggaaggctt tgacctgaac ctggcggatt tcaaagacat gatccagcag gtggcctttg
    70501 ccgcctccac ggatgaagcc cgcccggtgc tgcagggggt caaaatggaa gtggttgaaa
    70561 accatttcac gcttgccgcc acggatgggt ttcgcatttc tgtgcgcaaa gaatcgcttt
    70621 caaaccccgt tgagcaggaa ctttccgtta tcatccccgc ccgcgcgatg aatgaactgg
    70681 cgcggatttt cagcgaggtg gacaaatccg tgcgcatggt cgtaccgcag ggtcgaggac
    70741 aggtcatttt ccgtctgaaa gacgccgaac tggtttccca actcatcgac ggcaacttcc
    70801 ctgattacaa ggccatcatc cccaagagcg ccaagaccaa gaccatcatt tccacgccgg
    70861 ctttcctcaa agcctgccga caggctgaag tcatcgcgcg tgagggtaac aatgtggtcc
    70921 gcctggatat ccgacccgag gaggaaggcc caggcaaggt ggaaatcaac gctgttaccg
    70981 aagaaaccgg gcagagtgac atttctgttg aagcccacat cgagggcagt ggtctgctca
    71041 tcgcctttaa tgtgcgtttt ctgcgtgaag tgctggacgt gatccactca cctaacgtgg
    71101 cgcttgaaac caacgccaat aactcgcctg gcgttatccg cccaatgggt gaagaagatt
    71161 ttgttcacgt catcatgccg atgcaccttg gataatctcc aaacgcccct ctccttgcgg
    71221 agaggggttt ttggttctta atattttcta gaaataattg atggatttct cgcccaacac
    71281 ctcagaaagg ctgctcaaca tcacgttggc gctggcacag taccccattc tagcggggcg
    71341 catccgtgcc cgcatgcgtg aagaactatt cacccggcaa ataacggatg cgaagcgttt
    71401 tgaggcccgc gtgcacgaga acgccatcaa atctcaaggg ctcgaggggg tcagcaatcc
    71461 ctacagcgaa gaaagttccg acctgtggga aaggcgcaag tcgattgtgc gcgatcacct
    71521 taccgacctg ttgttcagcc gccacctgac cttcgagctg ctggagtcgc tgatcaagga
    71581 agtgttgaac gaaaggggca tttcttcgga tgagatgttg ttatcgatta acccagagct
    71641 ggcgccgctt gacctggtgt ttgaacaggc gctgttgatc gaaggcatgc cggaagaaaa
    71701 acgcgcccgt tacattgcca ggttagaaga atcaaaagtt gtgctcatcc gcacgctgat
    71761 cagtgatcag ttgcgctaca tcaatatcgc caaggaatgg ctgtccatcc aggacctggc
    71821 tgagattcgt cggcgcaaaa taggcggagg gcgcatcggc ggtaaatctg ccggcatgat
    71881 cctggcgcaa cacatcctgc gtaataacag cgacttgagc gacctccctt gtttatcgat
    71941 gccggagtct ttctatgtag gttcgaatga gttttacact ttcatgtcca tcaacaactt
    72001 gttgaagtgg aatgaccaga agtacaagac agaaactgaa atgcgcgatg attacccgca
    72061 cattgaagaa gagttcctta aaggcacttt ccctccagat atcctggacc agttgcagac
    72121 gctgctgatc aaaatcggca aacagccact cattgtgcgc tcctccagcc tgctggaaga
    72181 caactttggc acctctttcg ccggcaaata cgagagcgtc ttctgcccta accagggttc
    72241 tttggcagag aacctcaaag cgctcacccg ggcaattgcc caggtgtacg cctccacact
    72301 taaccccaac gcgttgctct accgccgcag caagggattg ctggattacg atgaacgcat
    72361 ggcgctgctc atccaatggg tagagggcga ccagtacggc gattacctga tgccacacgc
    72421 cgccggtgta gccttcagcc acaacctgta ccgctggtct ccgcaaatac gcagtgaaga
    72481 cggcttcatc cgcctggttt gggggttggg cacgcgcgcg gtcgaccgcg ttggtaatga
    72541 ttacccgcgg ctcatcgccc tcagccatcc gctattgcgg cccagcaatg acccacgctc
    72601 catccggcgt tactcgcagc agtatgtgga cttgatcgac ctgaagaaga atcagttcac
    72661 taccatgccc atccaccagg tgttggcagc ggactacccg ccgctgcgtt acctggccca
    72721 ggtagatgaa gacggctact ttgaatcgtt gcgttcaacc ctcatcagca acgtacagaa
    72781 cctggtgctc acctttgatg tcttgatcca gcgcactcct tttgcagaac gtatgaaaac
    72841 catcctgaaa tcgctggagc aggcttatca cgctccggta gatgtggaat ttaccgtcaa
    72901 cctgacggaa aacagcgtcg gcagcccgca attatgcatt accatcttgc agtgccgacc
    72961 gcagggcttt ctgatcgaaa ccgaggtcga acgcattccc ccagacctca cccccgagga
    73021 aattcttttc tcaaccgatt tcatggtacc gcaaggcaag gtcagtgctg tggattgggt
    73081 gctttatgtg cagccggaag cctactttgc gttgaagacc aacgcggaac gcgccgcttt
    73141 ggcgagaaca atcggtaaat tgaacgatgt gcttaaggac gaatctttta tttgcatcgg
    73201 accaggccga tggggtagtt ccaacgctga cctgggcgtg cccatcagtt acggagatat
    73261 ctaccacgcc cgcgcgctgg tggaaatggc tggagagcac tgcgggctgc cgcctgagcc
    73321 ctcgctgggc acgcacttct tccaggacct gctggaatct cagatctacc cgctggcgct
    73381 gcagctggat gacccggcga cggtcttcaa ccgctctttc tttgaccatg cgccaaaccg
    73441 gcttaacgag ctgctgcctg aagcggcggg ctttgccggg tgtttgcgcg tgctgcgcat
    73501 aagcgacagc taccccggtc aaacgttgcg cctgatcatg aacggggaaa tcggccgggc
    73561 agcaggtttc ctggtcaata cgcaggaata aaaaaaaccg ctcccttaat ggggagcggt
    73621 tagggtattt ttgctctcag cgcgggtctt cctcaaccgt accagctgct tcttcatcag
    73681 cgtcttcact gaacggttgt tcatcgcttt caacagccaa tggttcaacc tgcgcctgct
    73741 cttcaccaga ggggtcttcg tcgttatctt catcgtcctc gtccctgtct tcgtcgtaac
    73801 catcctcttc gccatctatg gtttgtacct catcctcgat ttccgcttct tccttccaga
    73861 aacggtacaa caggtaaata ttcagcagca ggaagatagc cagtacgccc tcaatcgacc
    73921 agaggctcca acctttactc ctcaggaata ccgccagcgc gtcaataacc atgtgataga
    73981 gaacggcgag cccgaaccat gtaaaagaac gatccgcaat cgatttccat accagtgtgg
    74041 ctaaaacaat ctgcgtggag atggctatca cgcgctcaac gcctggcagc aagcctgcgt
    74101 gccaggggtc gagccagaat cgacttatct gaaagagcat gtactgcaca tcattggttg
    74161 aaacgccttt tgcgatctgc gccccggggt tgtagaagag cactgtaaag atggggatag
    74221 cgatgcttaa aaccgctatc aaaatgctct ccgccccccc atgtcccacg ccgagcgcga
    74281 atgctgaacc atagcgctcc gttttcttct gcatcagttt aaatcccagc cagcgcgcgg
    74341 tttcttcaca aattcccgcc agcagaccag ccaggacagc gttgaccaca ggcacccatt
    74401 gcgcggcagg tacgctgaga atccccttgg aaaaaagggt gttgatacca gtaacgagag
    74461 ggatacgcag cgcttgcgag gcgaggaagg tgagcacccc tgccagcacg acccaccacg
    74521 atacgttgaa gcggcgaaca accaggaaag ccagcaatac cggcaatccg atcatcacta
    74581 tcaaagacaa cgcataagta atcagcaaag gaatattcga aatcatgcaa catgcctttc
    74641 ctcagattac gcggtcgcca gaccgtgcgc gagggattgc ccaagtatag tcgattttcg
    74701 cagtaatatc aggcgcacct tcttcaacct ccataaaaca ccttcttgtg gggcgcgcga
    74761 ttgtttcacg tgaaacaatc ctcaactggt cgctgtcaat agttgctcgc ggttgaaggc
    74821 gcgcacagca aaagagaaca aaatcacatc aaccacagcg atcgccagtc caattaacag
    74881 cccgaccagc acactcaggt agaccacccc ggtagcctgg ccagccagca aagccagcac
    74941 caccagcacc agtgagccgc tggtctgata cgcgcccata aaggtctgcg ttttagctga
    75001 aatgagcacg gtaaaagcaa tccccagcaa agaaatggcg ggggaaaccc ataaaaccaa
    75061 cgggtaccag ctgggcaaag ggaaccacag gcgctgcatt accggataag ccgtggcgtt
    75121 caccacgatg gtgtaacccg caaaactgac cagcgtgacc aacaccgccg ggatgaaacc
    75181 agccagcacc ttgccgatga acagctccgc gtcagtggcg ggtgtgtaaa gcaacgcttc
    75241 aatggtcttg cgttcacgtt caccagcgaa agactctgcg gcgatgaccg ttgagaacat
    75301 caacggcata atgagaaaga agggcgcaaa cagatagccc aaaacaagca caatgcccga
    75361 ctgtacttcg tttaacccgc gtatgagttc gatcataaac ggcggcatgc ggttaagaaa
    75421 catggcaaaa tccgggtcgc gcagggggct ttccagagcg gttcctgcgg atgagaaacc
    75481 aacgatcagc gccgccggta tgatgagcac gaagatggct ggcacgatca ggataggcag
    75541 ccagaccgcc ttgttttggc tggcttcctt aatgtctttc ttagcgatag tgaggatcgt
    75601 tttccagttc ataccgcacc ttcctgggct tgtgactgca acttgaagta aatctcttcc
    75661 agtgtaacct tttgaggttg tacgctcacg atctgggctt tttgggctac cagcgtcgcc
    75721 accagcgagg gaataatctc ctcttccgct acctgcgcgc gtattttgcg ctcgctgacc
    75781 tgcacatcga tcacgccgcg cacgcgcagg ctagaagggt cattttgcag gggctgcagt
    75841 aaatcgacct cgacccataa cccgtggttg aattgctgag tgagttcagg caggcttccc
    75901 agcgccagca gccggccgct gttgagaatc gccacgcggt ccgccaggcg ttcagcctcc
    75961 tctaaacggt gggtgcagag aaaaaccgtg ttttcttcct gacggcaaag ggattcaatg
    76021 agctcgtgca cctgctgcgt agactccggg tccaggctgg aggtgggctc atcgagaaag
    76081 aggattcgcg ggtgggtcag gaatgccctg gccagcgcca accgctgctt catgcctttg
    76141 ctgtaggcgc ctgcacgttc gctttccctg ttttttaatc caaagaactg tagaatttca
    76201 tccgttcttt ttaacagaag cgattctggt acgccgtaaa gccgtccaaa aaagagcagg
    76261 ttttcacgcg cgctgagccg ttcgtaaagc gcgctggttt cagtgagcac gcctgtttgc
    76321 tgtcgtattt ggctgccctg gcgtactgaa tccatgccca acacgttgat acgaccagag
    76381 gtgggggtat ataacccatt caccagccgg atggtggtgg ttttccccgc cccgtttggt
    76441 cccagcaggg caaatatttc cccctgcctg acctcgtatg agagatcttt taacgcgtac
    76501 ctgctgttaa agcggtggct tagatgctca atgacgattg catgctgcat aagtcaatcc
    76561 ttctctatgt ttcacgtgaa acatgatcat ccccaaccgc ctgatgggca gaagtggggc
    76621 acccggttga cttctttcgc aaatttccgg agttgcttag cactaatctt ttcattccat
    76681 ttctcgcatt ccaattttcg aagaagatac acccggggtg tttttccagc tgtgtaaaaa
    76741 atcgcgcagg cattcctcca cttcctgggg ttggtatccc tgccgcgcaa gggactcaaa
    76801 aaaacgccgc gcttgcgtca gcagcgcgcc gcgtttgagc tgttgttgac gattttcttc
    76861 cagccgttca agaatataag ttccacgacc ctgctgagtg gagatcagcc cggcgttatc
    76921 taacacgcgg taagccctgg cgatggtgtt gaaattcacc cgcaggtgcg cagccatgtg
    76981 cctcaccgtg ggcagttgtt cacccggctt caacaacccc tgaacaagct tgtgttgcac
    77041 ctgttcgaca atctgaatgt aagccggtat ccctgaacga aaatcaactg taaaatcact
    77101 caccttcaca ttcgtatctc ctcttcctat tgtatcattt tactattgta tcattgtttt
    77161 tccggtgatg caccacccag ggagtaaaac aggagtttcg tcatcttttc tcttcggtct
    77221 ttcaggttac aataaacgcg gggttattca tcctcatacc actcaaacat tgataaggag
    77281 agaacaatgt cagagattta cgacaacctg atggctgcct tcgctggaga gagccaggcg
    77341 aaccgcaagt acctggcgta tgccaaaaag gctgaggaag agggtcacac ccaggtggcc
    77401 cgccttttcc gtgccgtggc gcacgccgaa acgattcatg cgctcaacca tttccgctac
    77461 gccggcgagg tgggatcaac tgccgagaat ctgaacgcgg ctgctgctgg tgaaaaatac
    77521 gagagcacgg agatgtaccc cgcttttatc gaaaacgcca aaaaagcggg cgataaaaaa
    77581 gcgctcaata gtttccactg ggcgaatgag gctgaaaagg tccacgagat gctgtaccga
    77641 gaagcgctgg cttcgcttgg gcaaccagga gaggaatacg attactttat ctgcccggtt
    77701 tgcgggtaca ccgaaaaacg cagcgcgcct gaaaaatgcc cggtttgcgg cttccccggc
    77761 agccgtttcg agcgcatcag ctgagtgttt ccaagaaatc cctgccgagc agggattttt
    77821 ttatcgtgga ggcaaaggca aatcaggcgg gttttttctt ccaataattc aaggcgttgg
    77881 ttgcgacctg tactcgggag tggtaaaatc taattaatgg tcttaatgaa gacaaagttc
    77941 atgtttgtgg gataaaggag gttcgtattc ttatggaaca gatggtaggt tcctttgagg
    78001 tcaaaaaagg gctggcgcag atgttgaagg gcggcgtgat catggatgtg gtcaacgctg
    78061 accacgccaa gattgccgaa gatgccggcg cttgcgcggt gatggcgctg gaacgggtgc
    78121 ccgctgacat ccgcgcggat ggcggcgtgg cgcgcatgag cgaccccgat atgatccaaa
    78181 aaatcatgga atcggtcagt atccccgtga tggcaaaatg ccggattggt cactttgttg
    78241 aagcgcaaat actggaagcc attggtgtgg attacataga tgaatcggaa gttctcaccc
    78301 cggccgacga ggcgcaccat atttacaaac acgcgttcaa agtgcctttc gtttgcggct
    78361 gccgcaacct gggtgaagcc ctgcgccgca taggggaagg cgcggcgatg atccgcacca
    78421 agggagaagc gggcaccggt gatgtggttg aagccgtgcg ccatgctcgc tcagtactgg
    78481 gagagatccg caggttgcag cagatgcccg aggaagaagt gatggccttt gccaaagaga
    78541 taggcgcgcc ctatgagctg gtgaccgagg tgcgtacact tggtcgcctg ccagtggtca
    78601 acttcgctgc aggcggtatc gccaccccag cggacgcagc gctgatgatg caactgggtg
    78661 ttgatggcgt ttttgtgggt tcgggtatct ttaaatctgg cgacccagcc aagcgcgccg
    78721 cggccatcgt caaagccacc acccattaca acgaccccaa gatactggca gaagtcagcc
    78781 gcaatcttgg cgaagccatg gtagggcggc aaatctcagc catgcccgaa gaagagaaga
    78841 tcgccacgcg cggatggtaa gcggtagatg aaaatcggcg tcctggcgtt acagggtggt
    78901 tttgccgagc accaggccgt cctcgaggcc ctgggtgtag aagtggctca ggtgcgattg
    78961 ccccaacatc tcgccggtct cgatgggctc atcatccctg gtggcgaatc caccactatc
    79021 ggtagattgg cagtggattt tgatttgatg cagccgctgc gcctgttcgg tcgtgaacac
    79081 gccatatggg gcacctgtgc aggggcaatt tttctctcaa agaacgccgg tcgtgaacaa
    79141 ccccttttag gattgatgga cattaccatt gcgcgtaatg cttttggccg ccaggcggaa
    79201 agcttcgagg ctgatctgga tgtccccgct ctgcttcagg tagacccgct tgatcgtcct
    79261 tttcacacgg tgttcatccg cgccccgttg atcgaatcag ttggtcaggg ggtcgagata
    79321 ttctctcaac tggaagacgg tcgcattgtc gccgcacgcc aggggcattt actggcaacc
    79381 tcgtttcacc ctgaattgac cggcgacaac cgctttcacc gttacttttt ggagctggca
    79441 aaataaacgt ccgcccgatt gcctggtacg accttcccgc gatactcagc cggcgccgtc
    79501 aggtgatcag cctacacaag gctgcctatc tcattcacgg taacccgatt ggcatcgctg
    79561 gtgtattggc gcgcttgaac cccacggaaa ccatttatac cgcggtaatg gaacaccccg
    79621 aaaatgggcg caaatatttt tgccaggtta ttcaccctcc cagcagccgc tcggcgcgct
    79681 tttcttatct ggcgcccgac ctgatccaaa acgcggaaga ttacctgccg ctgatcgatt
    79741 ttttgtgctt tcaggctggt gaaatggggg cgcttaacgt gatcgcggaa attgaagagg
    79801 cgcaccccct gtttgaaacc ctgcgccgcg ccggtttttg cgtgttcagc tgggaagcca
    79861 tctggcgcct gccggacgaa cgaagcgacc cagggtcatc cgcccagtgg tgcgctcccg
    79921 ccgccgctga cctcaacaat gtgcgcaatc tttaccagac cctcgtgcca ccgctggtgc
    79981 agaacgctga gccgtttgtg aatggtaata cgccgcggct gttgttcaag caaaaagacg
    80041 annnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    80101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngatacctcc aatgagattg
    80161 gcggtgacgg cgatgtgccg cacccggctg tgggcaaggc gcggcgcatg caggtttcta
    80221 ctccttcact gcaacacgaa gtgatgatcg aagcggttga aaaccacaac cctgaagtgg
    80281 tcatcatcga tgaaattggt cgcgaacttg aagccatggc cgctcgcacc atcgctgagc
    80341 gcggtgttca attagtggcc accgcccacg gccgcacatt agaaaacctg ctgctcaacc
    80401 ccaccctttc tgacctggta ggcgggatcg aatcggttac cctgtcagat gaagaagccc
    80461 gccggcgcgg cacacagaag accgtgctcg aacgccgctc tccgcccacg tttgacatgc
    80521 tggtggagct gcaggagcgc gaccgcctgg cagtgcacca ggacgtggcc gccgcggtag
    80581 atacattggt gcgcggttat ccactgcaac ctgagattcg ctggcgcgat gccgctaacg
    80641 aggtgcacgt agaaaaagcc cctcagccgg cggctgcccg cggcatggcc cagggtaccc
    80701 ggcgcaccta ttctgccagc ggcgaagaga aatcagaacc gggttcacgc catgcagaaa
    80761 gccggtacct gcgtaacgcc tccgcaccct cactggctta cgagcaggct gttgatcttt
    80821 ctgagcgccg accgataaac aaaatacgga tctaccctta cggggtggcg cgtaatcgtc
    80881 tgcagcaggc cgcgcaccgc cttggggtgc ccgccctcat tgcccgcgat gtcagcgaag
    80941 cggatatcgt gatgactttg cgcgcctatt accgctcccg ccagcagccc atcactgaag
    81001 ccgaatcccg cggtatcccc atttatgtat taagggccaa caccatcaac cagatggaac
    81061 aatcgctggc cgaaatcttc aacctctccg aagggaataa ccagaacctg aacgaggtaa
    81121 ccaaccagac agaatctgcc attcaggcgg tgcttaacgg tcagcgctgg gtcgatttac
    81181 cgcccgcctc ttcatccatc cgccgtatcc aacatgagat ggcgcgccag gcgcagcttg
    81241 tttcgcactc ctacgggaaa gaccccaacc gcagagtgcg catcttccgg gaataggcga
    81301 aagaagacag catgttcatc acactggaag gtcctgacgg cagcggtaaa tccatacagg
    81361 tcccggctct ggcggaattc gtcagacagc tgggctacga cgtgctcacc acgcgtgaac
    81421 caggcggtac ttctatcggc gaccagatac gcgaagtgat catgagcatg aaaaacaagt
    81481 cgatgaaccc gcgcaccgag atcctgttgt tctgcgccgc ccgggcgcag atcgtatccg
    81541 aggtgatccg cccccaattg gaaaaaggcg tggtggtcat cagcgaccgt tacgcggatt
    81601 caaccctggc ttaccagggc tacggtcacg gtctcgacct caaggtgttg cgcggcatcc
    81661 tcgacttcgc tacagggggg ctgactcccg acctgaccct gctgctggat gtggatgtgg
    81721 aaaccggtct gtccaggcgg caacagggcg gcggcgaatg gaaccggttg gacgattatg
    81781 cgctggcgtt tcaccggcgc gtacgcgacg gatacctgga actggcagcc gctgaaccgc
    81841 agcgctgggt gcgcatcgac gccagtcagg cgccacaaat agtgcagcag cacatgcaag
    81901 aagccgtgtt gcggcgtctg cgaccgaaag tatagaaaca tccaggagaa agattgaaga
    81961 aactggcttg gctggtatgg gtatcactat tcctggttgc cgcttgcgcc ccggtcagtc
    82021 acgatagcgc gccgatcccc gcttctgtca ccccctctgc caccttacca tttacgcccg
    82081 cgcttttcag cacacccatt gcactctctc cgcaacccgc ggtgaccacg caaccaacgg
    82141 tggcgtttga ccgaagcccg gccgcgacct gcgaggttgt gtcactggtc tcagcaggcg
    82201 ttacgggtga tttcatactt ccccctgaag agcaggctga ttggagctac ggaagcccct
    82261 ccccagcggt gaccatcatc tcatattgtc aatatcaact ccaggcgtgc cgtttgctca
    82321 caaacagcct ggcagaactt cagcaacgct atcctgacga agtgcgcgtg gt
//
LOCUS       DAOTLW010000003        72160 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_399_length_72160_cov_11.268192, whole genome shotgun sequence.
ACCESSION   DAOTLW010000003 DAOTLW010000000
VERSION     DAOTLW010000003.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 72160)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 72160)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..72160
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_399_length_72160_cov_11.268192"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..426
                     /locus_tag="P5516_00855"
     rRNA            <1..426
                     /locus_tag="P5516_00855"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            506..622
                     /gene="rrf"
                     /locus_tag="P5516_00860"
     rRNA            506..622
                     /gene="rrf"
                     /locus_tag="P5516_00860"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
     gene            complement(794..1735)
                     /locus_tag="P5516_00865"
     CDS             complement(794..1735)
                     /locus_tag="P5516_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91018.1"
                     /translation="MSGLETTITDFLKLTFLSMVPFVLASQGTMLGGRTGVFNVAQEG
                     IMLVGASVGFLVSYTTGSNLIGALVAMAAGGLFGLVLAYFTTTLKMDQFVVGLALFFI
                     GTGLSSLLPKLVIGITLTPPLVKTLPEIPIPLLSQIPIIGPVLFKQNILVYFSILLSI
                     FLWYILFRTNMGLRLRSVGENPKASDALGINVVSNRYITSIIGGMLIGLAGAYLPMVY
                     TGTFTDLMTKGRGWIAIALTFFGAWSPLPILIGSLFFAAIEVLSFRFQVGGSFIPYQF
                     LSMLPYIATIIVMIFTFGKSRVPAFLGQNYDRERRTL"
     gene            complement(1732..2745)
                     /locus_tag="P5516_00870"
     CDS             complement(1732..2745)
                     /locus_tag="P5516_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91019.1"
                     /translation="MTQIKKFFPYLVSVLAAFLVVGVLLWALGFNPIKAYQTILFTSF
                     RTPNGFVQTLLKWIPLTLTALAFTVPLSSGKFNIGAEGQMLVGAIGATAVGILFKDLP
                     PALLLPLVVLAGMLAGSFWGLIAAWLLYQFNINEILSTVLLNFISFALVDFVATGPWR
                     DPAAGHPTTIPIGANGFLPMLVKNPPLHSGIILAVIVAIAVYIFTSKTTSGYELLVTG
                     ANPRASKVHGIDTRKMFLFSLVIGAAVAGLAGVIEAAGTHHRLIEGLHSNFPTLGLII
                     GLMARGKNQAVPFVAFFISILEVGGSAMQRTIMVPVQIVLIAEALILIFILLSDIIKR
                     RQK"
     gene            complement(2764..4311)
                     /locus_tag="P5516_00875"
     CDS             complement(2764..4311)
                     /locus_tag="P5516_00875"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91020.1"
                     /translation="MEQENVLQMQQVNKSFDRVVALCNVDFELKPKEILGLLGGNGAG
                     KTTLMNILYGLYRADSGTILLKGQPVTIHSPRDAIRQGIGMVHQHFLQINNYTVLENI
                     ILGSKVSHPLSANYQVEEAAIKELCKRFGLEIDLSARVETLPMGTRQKVEILKALYRG
                     VNILILDEPTTNLIPQEVDSLFETLKIMVREGLSIVFITHKLREVLSICDRITVLREG
                     KNVVSLTRKEASDEALVRAMVGDKMDLEKSVMFTSHEDSATVAGSQEVLSLEGVSLIS
                     QHKIQQLTDISFSIGSGEIFGVAGVAGNGQRELAEVVMGVQPVTSGKINFNGQEITTH
                     STKERIAEGFVYVPEDRLSDGFLNKASVAHNLILGYHNLEPYSKKGIINWKNVHENSR
                     KMISEYNIKTTGPDEIGGNLSGGNIQRVMIARAFSQPSRLMVAHNPTRGLDIPSMDFV
                     YQKLLGNSATGTAILLLSEDLDELLLICNRIAVMYRGKVVGILPREKFEKYEIGRLMS
                     GYEQESK"
     gene            complement(4416..5501)
                     /locus_tag="P5516_00880"
     CDS             complement(4416..5501)
                     /locus_tag="P5516_00880"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRT91021.1"
                     /translation="MKKNLLFAISVLLVLSVLLSACAKPTAAPVATEPPAVQETEAPA
                     ATEAPAAQEPAMEVKLAAIFPGVITDNDYNTLAYVGMTAAQEQLGIQAVYSESVPVPD
                     VDRVMREYIDAGFNVIWTHGGQFVNQTAELAKLFPEVVFIAEGDAPIADPSPNLWFID
                     RNFNVGFYGIGAAAALASQTGKIAYLGGQALPFSYQEVHAIQQAISDLDIKVELVPVW
                     TADFNDPAKTRQLADALIADGVDVIMGSVNLGMVGVFEAAKATTDKKILITAKYSDKT
                     SFGPDNYITAVLYDFSKPLVDIITSIEGGKTGGYYPLGFDTGVALQLPLQNVTPEVAA
                     KVEEVINKVISGEIVVVKDSTEVTSAK"
     gene            5938..6138
                     /gene="rpsU"
                     /locus_tag="P5516_00885"
     CDS             5938..6138
                     /gene="rpsU"
                     /locus_tag="P5516_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HRT91022.1"
                     /translation="MASVVLRPNESQDSLLKRFRKKVVKSGVLSTIRRKRWFVSKSEL
                     RRVEKKKAIRRIKRRQYTRVGE"
     gene            6185..6439
                     /gene="infA"
                     /locus_tag="P5516_00890"
     CDS             6185..6439
                     /gene="infA"
                     /locus_tag="P5516_00890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HRT91023.1"
                     /translation="MAKEEDKIQIEGEIIEALPGTQFRVRLENGHEVLAYLSGRMRRY
                     YIRILLGDRVKVEMSPYDLTRGRIVYRQKKNEPILDEPTR"
     gene            complement(6486..>8018)
                     /locus_tag="P5516_00895"
     CDS             complement(6486..>8018)
                     /locus_tag="P5516_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRT91024.1"
                     /translation="NLALEIPVWETDLCIQCGKCAFVCPHAVIRQKVYDPSYLKDAPP
                     TFKSIEAKFKEFPGMKYTIQVAPEDCTGCSLCVEACPARDKNNPLRKAINMAPQLPLR
                     EQEAINWDYFLSIPAPDPDLYQPTTVKNSQLVEPLFEFSGACAGCGETPYIKLVTQLF
                     GDRTIVANATGCSSIYGANLPTTPWAVNNQGRGPAWANSLFEDNAEFGLGMRLTLDQQ
                     NAFARELLAKMAGELGTTLVDEILNADQSTETGVRQQRKRVEALKEKLATSKDANASM
                     LLGVADALVKKSVWIIGGDGWAYDIGYGGLDHVLSSGRNVNILVLDTEVYSNTGGQSS
                     KATPLAAVAKFSAKGKGLGKKDLGLMAMSYGYVYVANVAMGANDNQTLKAFLEADAYD
                     GPALIIAYSHCIAHGIDMQKGLEQQKLLAATGLWLLFRYNPTLVNEGKNPLQLDSREP
                     TVAVKDYAYNETRYRMLVQSDENRAEMLMNEAAQFAQRKWDLYKQMAAMAYSKPGEEK
                     EQ"
     assembly_gap    8019..8118
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<8119..9785)
                     /gene="nifJ"
                     /locus_tag="P5516_00900"
     CDS             complement(<8119..9785)
                     /gene="nifJ"
                     /locus_tag="P5516_00900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HRT91025.1"
                     /translation="MKRELITIDANEAVANIAYRLAEVIAIYPITPSSSMGEFSDAWM
                     AAGVRNLWGTVPTVIEMQSEGGAAGAVHGALQAGALSTTFTASQGLLLMIPNMYKIAG
                     ELTSTVFHVAARTVATHALSIYGDHSDVMAARQTGFAFLASDSVQTAQDQALIANAAT
                     LEARVPFVHFFDGFRTSAEINKIERLCEDDLKAMIDEKLISAVRARALSPERPVIRGT
                     AQNPDVFFQGRETANRFYARTPDIVQNAMDKFAKLTGRSYHLFDYVGAPDATEVLVLM
                     GSGAETAEETVKFLTQQGRKVGVVNVRLYRPFSVAHFVSALPATVKTIAVLDRTKEPG
                     AIGEPLYQDVVTAVNEALSTGKTPFKVAPRIIGGRYGLSSKEFTPAMVNGVFNEMVKA
                     EPKNHFTVGIVDDVTHTSLDYDHKFCIAHEKTIRCVFFGLGSDGTVGANKNSIKIIGE
                     ETNNYAQGFFYYDSKKSGSVTISHLRFGPHQIRAPYLIEKEDAQFVACHQFSFLEWLD
                     MLKYAAPGAIFLLNSVYDRDEVWDMLPIEVQQEIIEKKLKFYMINAN"
     gene            complement(9925..10452)
                     /locus_tag="P5516_00905"
     CDS             complement(9925..10452)
                     /locus_tag="P5516_00905"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRT91026.1"
                     /translation="MTYTIHHESIEHRTPLFTVKRVDLSLPDGKPRVLDLIEIQNAVT
                     ILPLDSEGYIHFVKQYRIGARSELLELPAGKIENNEDPLQTAQRELREEIGMSASSWR
                     ALGNFYLSPGYASEYMYGFLAQDLVPDPLAPDADEFLNIIKVSLPDALAMVRRGEIQD
                     SKTLAVLMLAHPLLS"
     gene            complement(10581..11219)
                     /locus_tag="P5516_00910"
     CDS             complement(10581..11219)
                     /locus_tag="P5516_00910"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT91027.1"
                     /translation="MKKRILSWVILLVIGALLMTACERAATRAPRDTTRANDEIPFPV
                     STQSQIMIDILAATQTALAVEGTVESENPQALAQAQPAAMPTPMPQPEEEEPEPTPGL
                     PSTYTIQKDEFLFCIARRLNVNASEFLAINGLGVDYYAQVGDRLTIPQSGKPFDGPRA
                     LRAHPVDYTVAAGDSIGKIACTFGDVSPEAIYNANGLEVGSSLKPGQVLHIP"
     gene            11383..11832
                     /locus_tag="P5516_00915"
     CDS             11383..11832
                     /locus_tag="P5516_00915"
                     /inference="COORDINATES: protein motif:HMM:NF016874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside 2-deoxyribosyltransferase"
                     /protein_id="HRT91028.1"
                     /translation="MKIYFSCSITGGRSEESVYQKIVSALDSMGYEVPTAHLASPEVV
                     EMEKTADPNDIFERDMAWLRECDAVIAEVSSPSHGVGYEIAYALTLGKPVLCCYQAGR
                     SVSKIITGNTSPGLSVMTYIVEEDAPVVVREFIQRLGNRPGVQESEY"
     gene            11897..12835
                     /locus_tag="P5516_00920"
     CDS             11897..12835
                     /locus_tag="P5516_00920"
                     /inference="COORDINATES: protein motif:HMM:NF013705.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="J domain-containing protein"
                     /protein_id="HRT91029.1"
                     /translation="MEYKDYYQVLGVDRKATPEDIKKSFRKLAMKYHPDRNKDNKQAE
                     EKFKEINEAYEVLSDPKKRERYDQLGSSYQQWQQRGGNPGNFNWNEWYTNQGGRAQQV
                     NPEDFGDIFGGFSDFFRVIFGGMPVSGRQPTRQPGTQQSAQARRAANYEQKVQITLEE
                     AYRGTQRILQINERRIEVKIPAGARTGTKVRVAGAGPADAYGRGSDIYLVIELLPHAR
                     FKISGSDLTTEVKIDYLTAVLGGTVTVGTLSGDVVLTIPSGTQPGQKFRLSGRGMPIL
                     KSSQTYGNLYVQVKVELPKKLTDEQRKLFEQLRKHS"
     gene            12854..14026
                     /locus_tag="P5516_00925"
     CDS             12854..14026
                     /locus_tag="P5516_00925"
                     /inference="COORDINATES: protein motif:HMM:NF012318.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HRT91030.1"
                     /translation="MRKPVVLSIALLLLVVLMACVFIPSQDWNPSDVLENVVTKVPDI
                     VSPNVPPVFVNAEDLANVDQVLTTLYERVNPGVVTIIATTRQGEGSGSGFVYDYEGHI
                     LTNYHVVKDAETIEVDFASGKKIYGDVIATDVDSDIAVIHVDMPAAELVPLSLGDSDQ
                     LKVGQMVVAIGNPYRLTSTMTLGVVSAKGRILDSLRTTEDFTSYTAGDLIQTDAAINP
                     GNSGGPLLNLAGQVVGINRAIRTSGTTLEGGAVNTGIGYAVSINIVKRVVPILISKGS
                     YDYPLMGISSSSNELTLSMWKKISHTVTSGVYVVSVTPGGPADRAGLVGGTQATDVQG
                     FYSGGDIITAVDGLPVNIYGDLISYIFKNKSPGDVIVLTIIRNDREMEVPLTLGSR"
     gene            14071..14820
                     /locus_tag="P5516_00930"
     CDS             14071..14820
                     /locus_tag="P5516_00930"
                     /inference="COORDINATES: protein motif:HMM:NF037429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91031.1"
                     /translation="MFTESSLRKIQDFSADYPVVSLYLNTEPSRGNAETHRLRLRNMI
                     KDISLKQDAELIEKFFSYTYDWSGRSVAVFSCAPADFFHAIPLAVPVKDFIQIGSKAA
                     VAPLEDLLQDYSNLGVILVDKQGARLFHFHMGELVEQQGFLGDQVKQVKAGGASSAHG
                     LRGGALDGGRAMRETIDRNQREVAETAARFFESKRVRRIMLGGTDENISRFKSHLPKS
                     MQSLVVGSFAMGMTASNAEVLQKVLDQALEK"
     gene            complement(14884..15633)
                     /locus_tag="P5516_00935"
     CDS             complement(14884..15633)
                     /locus_tag="P5516_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91032.1"
                     /translation="MEPQKEPQPQDSPETTEPLPGFEPATQGTSSSQEATAEPVPEVK
                     DKPVLSPSQPVKKSFWQKVLPWVIVALVFYVGGLATLFFAVYQPAKQAAAAAAASAEK
                     EITSLNSQVSQLELQNSQVQQELETTRANLVDTQDELTTAQATIEKQLVEVANANIKR
                     LAYKLMGNINTARAALEKGDTNSARQALNFASKDLSDLQVAGLTPEALSGFSNWLNEA
                     VANLEETTLQKSRAALDMLYTNLLLLVDNLP"
     gene            15768..17387
                     /gene="murJ"
                     /locus_tag="P5516_00940"
     CDS             15768..17387
                     /gene="murJ"
                     /locus_tag="P5516_00940"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRT91033.1"
                     /translation="MDAATLPGNTENRRIARDAGTVMLAILLGQVFSLLSSILITRAF
                     GTGIQNEAFNASNRLPDILYQLIAGGALASAFIPTFTTLLTRGERSKAWLLASAIANI
                     ITVIFIVLGVLIALFTPWVVQRILAPGFAADPVKFALTVDLVRIQMIAPVIFGLSGLG
                     MGILNSHRSFLWPALAPMMYSIGKILGVLLLAPSLGVYGLALGVVGGALMHGLIQMPA
                     LLKLPGLKYTPTFGIRLPDVREVARLMGPRVLGVAFVQLNFLVNTRLASLQPLGSVTA
                     LAVGFSLMMIPEAAIAQAIAIAALPAFSAQAAAGKLEEMRNSLAALLRGLLLLALPAS
                     IGLILLRAPLVTIIFQRGEFDASSTQLVAWALLWYATGLVGHSVVEIVSRAFYALHDT
                     KTPVGVGVAAMSLNVGLSIGLSRLFNTWGWMPHGGLALANSIATFLEMGALLIFMRRR
                     LTGLEGRHVLNGLVKALAAAGVMTLALWGWMRATAGASAWLVALGGMLVGALVYVLML
                     ALLRVPELKLAWVGTKRRLLKRTSGESDEKQ"
     gene            17374..18126
                     /locus_tag="P5516_00945"
     CDS             17374..18126
                     /locus_tag="P5516_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SagB/ThcOx family dehydrogenase"
                     /protein_id="HRT91034.1"
                     /translation="MKNNSGHEFMVKTRYANISPSPQNSGVVPQPPLELALPDGAQLL
                     PLPRPADLVLAPMELRAAIEARRSIRAYAETPLSLAELTYLLWVSQGVKRVSARPSTA
                     RNVPSAGARHAFETYLLINRVAGLTPGLYRYQAIEHGLLRLETAADIAGSIAHACIDQ
                     PQLTGSAAAFIWAAVVERMFWRYVERGYRYLHLDAGHVCQNLALGAEQLGCGICPIAA
                     FDDELLNNTLGLDGEEMFVVYLATVGKKTDKK"
     gene            complement(18150..19145)
                     /gene="pfkA"
                     /locus_tag="P5516_00950"
     CDS             complement(18150..19145)
                     /gene="pfkA"
                     /locus_tag="P5516_00950"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HRT91035.1"
                     /translation="MNTMNEPVKSIKKIAVMTSGGDAPGMNPFIRAVVRTAYANNITV
                     LGVERGYEGLIQGKFRELRIREVGGILQRGGTILQTARCTEFREAGSQREALRHINNA
                     EIDALVVAGGDGSLTGALKLAQKGVPVIGVPASIDNDIYGTDMCIGVDTALNTIVDAI
                     DKLRDTASSHNRAFLVETMGRTSGYLAVQAGIITGAELVLIPEHKTPPREVAQAIEEA
                     YRRGKTHAIVVVAEGYKPPTTELGAMIDAMDIGFTTRVTILGHVQRGGKPTAFDRMLA
                     TRFGVKAVQFLLEGKSAVMTALSGREIIPLPMEEVINHKKVIANDYIEMASMLAR"
     gene            complement(19156..22977)
                     /locus_tag="P5516_00955"
     CDS             complement(19156..22977)
                     /locus_tag="P5516_00955"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF005298.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRT91036.1"
                     /translation="MSFAHLHVHTEYSLLDGFSNIKKLIQKVKDLGMDSIAITDHGTM
                     FGVIEFFSTAVEAGVKPIIGVEAYVAARRMSDRDPQKDKHSYHLVLLAENEIGYKNLL
                     QIATAGQLEGFYYYPRIDHEFLENHKEGLIATTSCMSGEVPRTILNKGVEAGQRVLEW
                     YINTFGKDHFFIELQNHPLRELPELNRTLIDLGKRYNLRCVATNDLHYIEQEDAHLQD
                     IKLAIQTGSLLSDPNRMKMGANTFYLRSPQEMAALFPDIPSALTNTLEVAERCNVNLI
                     QKGYHLPLFTVPDGFTPQTYLKKLCEDGLQVKYPGRVDDPEVRQRLEHELKIIHEMGF
                     DAYFLIVWDLCRYAAENNVWYNTRGSGAGSIVAYTLNITLIDPLKHGLFFERFLNPSR
                     IEMPDIDLDFEDDKRSLILQYCAEKYGSDKVAQIITFGTLGARAAIRDVGRVMDVPLS
                     EVDRISKTVPAMFPEEAVTIPRSLEKCPEFQTVYSEGGYVKELIDTACRMEGVARNAG
                     THAAGVVITDKPLLEYLPLHRPTSNAEESPIKSVTQFEMNIITKLGLLKVDFLGLSTL
                     TIIHKACDLIKKRHKIGLNLHNIPLDDPETFEFISQGRTAGLFQLEGSGMTRYIMQMK
                     PHNLANIIDMVALFRPGPMEKIPNYINRMHGEEEITYEHPLMEPIFKDTFGLPVYQEQ
                     LMRAVIELAGYTASEADNLRKAISKKNKNAIDEHRVKFIAGAQKNQISAVQAEEIFSG
                     WDNFARYGFNKSHAADYGVIAVETAYLKKHFPVEYMTALLSANKDESTKVAFYVADAR
                     SMGIEILPPDVNNSEWDFCIEDDPDGRTAIRFGLGAIKNVGIGPVDLIFQARQDGGFK
                     DLNDFIRRVDLHSVGKRSLECLIKVGALDRFGPRRALLEVADTLVSVSNSHFRAAECG
                     QMSIFGNATGLEDEIHLPVGVQLDRRLQLEWEKELSGMYISDHPISHYLPLIRQKATH
                     ATKELVEAEHKQKVSVAGMVTRIRSLTTKTGNQMAFATIEDLQGEVELVIFPKAWERM
                     GALVKMESVILVEGKVDLDKSDPKILVDVIKALREEDIPTDETPSDRPFAHGQEGTWQ
                     TDIDTIRYTDQAESYEEPPISEEESSWQVPTPPADHHAAPPTPDTDVVPSPATDLPQE
                     EAVSPSAQQPRENVETKFDTPPRDYILPPVIMSPIKTPPNTAAPGGTRLVTVTIKSSG
                     EKERDTRRIHRAHGLLNSFPGQDRFCFLVYEHGYRHLLDFPNHTTNATKELVEQLAEL
                     VGRENVQVENL"
     gene            complement(23179..23784)
                     /locus_tag="P5516_00960"
     CDS             complement(23179..23784)
                     /locus_tag="P5516_00960"
                     /inference="COORDINATES: protein motif:HMM:NF014038.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRT91037.1"
                     /translation="MEVKFFLSDLPALRDRLLSLGAEQKQARTFEKNERFDTPDGALA
                     LSGRALRLRHDLRDILTFKGPSSIIDGVRVRPEHEVFIDNPDKARLILEGLGYEMTVR
                     YEKWRTVYLLNDLFITLDELPYGHFIEIEGADTALIRATAGALALDWDARIEASYLEL
                     FERLKQSKGLTMRNLTFADFAGITFADSDLGVRPADCPFLS"
     gene            complement(23808..24254)
                     /locus_tag="P5516_00965"
     CDS             complement(23808..24254)
                     /locus_tag="P5516_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91038.1"
                     /translation="MEKTTAPHPPFLLHLFASAFLLVFLINLAGFLQALRSWNWLLAA
                     GVFPHPVYLLFKTVLLALLSFTAALAIWGRRAFAPRLGQANSLLFFLWYWFDRLVLTR
                     NPLPFKNQLLPLLVSVLLLLFVLVSAWLLEPYMKETRKPPHDVPGG"
     gene            24365..24571
                     /locus_tag="P5516_00970"
     CDS             24365..24571
                     /locus_tag="P5516_00970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91039.1"
                     /translation="MKISIWDILTVVLLLTALVLLVVVMVIFIHPDSRINPFPFPSLP
                     ATIVIPTLTPTLLQMPPTWTPTPF"
     gene            24645..24965
                     /locus_tag="P5516_00975"
     CDS             24645..24965
                     /locus_tag="P5516_00975"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRT91040.1"
                     /translation="MSEQITKEVFDHLVGLAALQLEMEQAEYLRKQLNNQLKAIAELE
                     AIPLEKDVPISLHGVPYEASLSAPPREDEWKPYPDPAGILAQAPQVVDGYIVVPDIPH
                     TELK"
     gene            24971..26566
                     /gene="gatA"
                     /locus_tag="P5516_00980"
     CDS             24971..26566
                     /gene="gatA"
                     /locus_tag="P5516_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRT91041.1"
                     /translation="MEICDLNALELARLVRSRQTSAVEVVKSCLERVAQVDGRPGSLE
                     QMPLSDEDKKRVHAFITLTGENALQQAEKVDQMLAAGEDPGVLAGVPFTVKDIFCVQD
                     TLTTAASKILANFTAPYTATPVQRLQDAGAILLGKVNLDEFTYGSSNESSAYQPSPRN
                     PWNTDRVPGGSSGGSAASVAAGEAPLSLGTDTAGSIRQPAAFCGVVGVKPTYGRVSRY
                     GLIAFASSLDCPGPVARDVKDAAAMLGVIAGSDERDATAARLAVPDYLEGIEEGVKGL
                     RIGLSPDYFRITYPDPASGELLSQPLPEEVARAVNEAAEKLSTMGADIVEGVPMPNTR
                     FGIPAYFVISRVEAASNLHRYDGVKYGLRSEAPTADLKEMYKITRNQGFGPQPKLRIL
                     MGMYVSAAQYSEQYYRRALQVRTLIRQDFEAIFDPAGAYRLDALLTPTTPTTAFKMAA
                     LYGDSVLMQYADQLTVPANHAGVPGISLPAGLDNEGLPIGIQLLGADYAEKTLFRAAR
                     AYEMTTQEADWRKQKPEVLRGRS"
     gene            26563..28029
                     /gene="gatB"
                     /locus_tag="P5516_00985"
     CDS             26563..28029
                     /gene="gatB"
                     /locus_tag="P5516_00985"
                     /inference="COORDINATES: protein motif:HMM:NF004012.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRT91042.1"
                     /translation="MTDYEAVIGLETHIQLNTVTKVFCSCKADSWFDPPNTNICPVCC
                     GLPGVLPVLNKAVVEKGVLLARAMNAEIRPLSFFDRKNYFYPDLPKGYQISQFDQSLA
                     RGGYLDLPIPAAASPDKQAYTRHVTIWKLHIEEDAGKTKVSNQRRLIDFNRCGVPLVE
                     MVTGPDLRTADEAAQYLIRLRQLLRWLGISEADMEKAHLRCDANVSIRVRGETKLNPK
                     TEIKNVNSIDAVREAIAREIERQIREVEAGRHIEAWTLEWDEDSGTLKKMRSKETEAD
                     YRYFREPDLLPIRLDEGWQRQILADFPELPLERRARFVEQYNLPEYDADILTSERKLS
                     DYYENTVKSYGGDPKKVSNWLMNDVLRMINDQGLNPQEMQLTPDALAEILKLVDAGTI
                     NINTGKALLQKVQESGKKPGAIVAEEGLGLVSDDSTIREVCQAVLAESPNEVAAYRAG
                     KVTLIGWFVGGVMKKMRGKADASLARQILEDLLNEKNR"
     gene            complement(28094..28573)
                     /locus_tag="P5516_00990"
     CDS             complement(28094..28573)
                     /locus_tag="P5516_00990"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HRT91043.1"
                     /translation="MDILWSPWRMKYVTDASNSGACVFCTSPGQADSVANLIVHRGQK
                     VYAILNRYPYTSGHLMVVPFAHVSSIELLTPDVRFEMIEMVNQAILVLRKIYQPDGFN
                     IGINMGVAAGAGIAEHAHIHIVPRWNGDTSFMSTTGETRVLPEELQETFKRVSAGWP"
     gene            28694..29944
                     /gene="xseA"
                     /locus_tag="P5516_00995"
     CDS             28694..29944
                     /gene="xseA"
                     /locus_tag="P5516_00995"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HRT91044.1"
                     /translation="MLQPPLFPVKTLSVSEITRYLRALFESDEILSDVWVQGEVSNLS
                     RPSLGHIYFTLKDAFSSIKCVIWKQTAWRLPLTLQNGMAVEAHGGVDIYERDGQYQLY
                     VDALRLTGEGALYQEFLRLKTRLEAEGLFALERKRPLPEQPSAIGLVTSSSGAALQDI
                     LNTLKRRFPLVQVILAPCAVQGEDAPPQIVKSLAALNAQNQADVIIVARGGGSLEDLW
                     AFNDEGVVRAITDSQIPVITGIGHETDFTLADFAADVRAATPTAAAEQVVPDAAEIKV
                     KLSDMANLLSATIRQICGAQRGRVQDSARRLELQSPLWRVRNDRQHIDELQARIANNQ
                     AHRLELMRSNLVHLQKRLVNLSPQAVLQRGYAIIKTAEGVLVKSVRQVHRGDVLDIRV
                     ADGAILSKVLTEPTTERNGGIADG"
     gene            29937..30167
                     /gene="xseB"
                     /locus_tag="P5516_01000"
     CDS             29937..30167
                     /gene="xseB"
                     /locus_tag="P5516_01000"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HRT91045.1"
                     /translation="MVKSASTLSDLSYEQAFSELETIVAALESEQKPLDEALQLYERG
                     QLLVQYCAALLDQADLRIQQLNPNKETTEDAA"
     gene            30164..30613
                     /locus_tag="P5516_01005"
     CDS             30164..30613
                     /locus_tag="P5516_01005"
                     /inference="COORDINATES: protein motif:HMM:NF014711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent PAP2 family protein"
                     /protein_id="HRT91046.1"
                     /translation="MSILAGYPELIACIMGWLVSSLLKIPTYYSIHRRINLKQAFGTG
                     GMPSSHAATVTATTLAIGLFSGFDHPAFALAVAVSMIVIYDAAGVRREAGYHAVIINR
                     LIDEYVKGPLIDQKKLKEMIGHTPLEVVGGVISGTLTTLILWLIWPK"
     gene            complement(30683..32431)
                     /locus_tag="P5516_01010"
     CDS             complement(30683..32431)
                     /locus_tag="P5516_01010"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT91047.1"
                     /translation="MTTADLNRTLSPSDPGDRPIGYVVGGGLKANLFVRLTLPAQEVQ
                     EGGFVVLESGNWQFYGLVTDLQLGALDPRYAELQNAARLSQPAARLLASQTLYTNLEV
                     MPALMLERGPEPGSAEYEDWAREHQGENAPRPLPIKTVPSHHAVARLASAGDVAEIFG
                     DPESKGFFIIGRTREQDHPVCINLDKFVQRSSGIFGATGTGKSFLTRIILAGLIQHNA
                     ASVLVLDMHNEYGFDDTSSDTGQAVPGLKGKYAERVRVVGLGRETTIRKRTPDLHLEI
                     YESDIQPEDIELLSRELNLRETTPTTLEALVHSFGPNRWYTEFKNMANGATIIDDEGK
                     KVPAPGSVAFWANQEGVNVAAAEGLHSKLRNVFNRPYIIERDPADKTAVNSLNNIIDT
                     LKAGKHVVLSFGDFEKDLDYLLVSNLLTRKIRESWEKMTNDFRSSGQNEPRPLVIAVE
                     EAHKILNREMASQTTFSTIAREMRKYYVTLLIIDQRPSQIYDEVMSQLGTRVSGWLGD
                     ESDIAAVLSGLAGRDSLRGMLARLQPKEEVLLLGWGVPMPILVRSRRYDNRFWEELKG
                     KNAKPDVEKSLSELGF"
     gene            complement(32446..33048)
                     /locus_tag="P5516_01015"
     CDS             complement(32446..33048)
                     /locus_tag="P5516_01015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91048.1"
                     /translation="MNAIPIGRLLRANTTSCVMGCRVSQAGTPPFGAMVLIPLSDGTR
                     IYGLVYDIHIDDDGLVRQLVSAQVSEEVLEDNRQNRNVPIEISLVFIGWEDSAGRVSH
                     ARVPRPPLTLDEIFLCSDADILRFTSNARLGYLRGLLRNQEIPAADLVTAHLVQVFKV
                     AHHPDPEGWRDRCLNEVIALLKDDYPQLMDVLSAVSVVME"
     gene            complement(33045..34154)
                     /locus_tag="P5516_01020"
     CDS             complement(33045..34154)
                     /locus_tag="P5516_01020"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HRT91049.1"
                     /translation="MGRDALSRAGEIRQSLEQLSVRLNENAENLKGLQRQVEEAAAKT
                     KGLRCAVPLHESLNAHFPCTLPAPASTIFAADGSQINPDPHDSVLYGLVNIGVFCIQP
                     HSGRVPLERTESHLLFGDELYASGGMASEDLVALLRDVQERRTLAQLAHLATPPVITL
                     TDGPLELYHEPRTDAAFKEKFEDYLRALDDLAIADVITAGYVSRPRADLVVRLLTLNE
                     ESSQPTVSGISDIALFHSILQPGERSAIFRLHSRSADEYKDRKALHFFYLNVGSPSRS
                     AFARVEIPQWVAENPQSVQTLHTTLVEQSALSGEAPYPYPLIRAHEIAVVKLSDRQQL
                     TTMIESELARQGILPGSRSEKQVHKDHPGRTRINP"
     gene            34361..34948
                     /locus_tag="P5516_01025"
     CDS             34361..34948
                     /locus_tag="P5516_01025"
                     /inference="COORDINATES: protein motif:HMM:NF014689.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HRT91050.1"
                     /translation="MRLLETTRMNSWEILLIALGLAMDCFAVSLGIGTNGMKKTARMV
                     LRLAFHFGLFQGGMAFIGWLAGRQIAGLISSFDHWLAFFLLLWVGGRMVIGSTKQEEE
                     CRKTDPTRGGSLVALSVATSIDALAVGLSLAIVDGSIFISSSIIGLTSFALSVSGYLL
                     GDKLGCRFGKRMELLGGLLLIGIGIRVLLSHLLGT"
     gene            35027..35347
                     /locus_tag="P5516_01030"
     CDS             35027..35347
                     /locus_tag="P5516_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT91051.1"
                     /translation="MPVYTYRCENCGIQFDQTQKFSDPALTRCPECGKKSLKKVYTPV
                     GIVFKGSGFYATDHRSPSGAAHNSNTKHEEAKSTEKTETKTETKAASQTTVESTSTPS
                     GDKA"
     gene            35500..35649
                     /locus_tag="P5516_01035"
     CDS             35500..35649
                     /locus_tag="P5516_01035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91052.1"
                     /translation="MAKPNKQTGAQKRSKITSSQRKMRAQQIILAVIGIVVIISMILA
                     LTMKF"
     gene            35657..37228
                     /locus_tag="P5516_01040"
     CDS             35657..37228
                     /locus_tag="P5516_01040"
                     /inference="COORDINATES: protein motif:HMM:NF025150.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4173 domain-containing protein"
                     /protein_id="HRT91053.1"
                     /translation="MPKKQKWFWLAALIAAWLFDFLFWSKGSGVSFFIWIAILLLAGY
                     TLAWSEGKKPSALSVLLTCFILGFAAVIALRNEPLTRAVSIFLTLNGMLLLTATFLNG
                     YWPWYRIKDYVVELVRVIGAGFSGAIWLLAKGKTPPPVDGSPAKSGWSKAWPVIRGLL
                     IALPVVAVLGLLLASADQVFSDWVKRILNIEKLPEYLFRLFYILIIAGFLVGVYLKAI
                     YPRKTAQKPEPNQAWLQPFLGWTESGIVLGAVNLLFIIFVIIQVRYLFGGEANISETG
                     FTYAEYARRGFSELVAVAVLSLGLYLLLGTVTKTESQGSKVGFTALAVLLMVNVLVIL
                     ASSFLRLMLYENAYGFSRPRTYTHIFIYWLAGLILAVMVLELMKRRGRIALALLVAVV
                     GFGATMAILNIDGFIVQRNVARAEAGAELDVVHLTTLSADAVPQLLQKYQSPELRKEV
                     REGLGATLACMEKAIQDPADLPWQEYNFGQARAAQLLRENADSWSQYKAYQTDRDWVV
                     RLNGQRIYCAYFIMD"
     gene            complement(37294..37665)
                     /locus_tag="P5516_01045"
     CDS             complement(37294..37665)
                     /locus_tag="P5516_01045"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HRT91054.1"
                     /translation="MEEEPRLYELIYAIVRQVPRGRVATYGQIARIAGRCSAQMVGFA
                     LAALPVYRGRDIAEEVPWQRIINAQGKISPRGFGMGSQRQRVLLEEEGILFDDQGSID
                     LSVFGWDGVLFSPVKNELDLI"
     gene            37732..>37982
                     /locus_tag="P5516_01050"
     CDS             37732..>37982
                     /locus_tag="P5516_01050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HRT91055.1"
                     /translation="MKRWQFWVGLLISVAFLYFAVRGLNLADFWKAVKDANYWWILPG
                     VAVFFAGLWVRAWRWHYLLGPVKKIPTRIMFPITCIGYMG"
     assembly_gap    37983..38082
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <38083..38661
                     /locus_tag="P5516_01055"
     CDS             <38083..38661
                     /locus_tag="P5516_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRT91056.1"
                     /translation="TAIFLAALLVFLLAAMFPRTTARLGGWFIERLLPPRAREKTTGI
                     MLKFLDGLESLRSPFNILMVFFTSVIIWLLETVKYWFVMQAFTFSVSFFALMLLNGIA
                     NLATTIPSAPGYIGTWEAVTRAVLVAFGVPGAEALGYGVVLHVALWLPVTLVGAYLMT
                     REGIRWSDSLRQEARSGEAEAGGEICDKESNK"
     gene            38658..39950
                     /locus_tag="P5516_01060"
     CDS             38658..39950
                     /locus_tag="P5516_01060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRT91057.1"
                     /translation="MKIAVIGAGFGGLAAAYDLVKAGNTVTLYEAESAPGGLAAGFKE
                     PNWEWSVEKYYHHWFTSDEHMFRLFRELGVEDRVMILSPKTVMYHKGKFYPFDSITSA
                     LLYPGLGYGLNKIRFGLVGLYLRFTNRWQPMEQTTVAEWMKKWAGEKVYTQMWEPLVM
                     GKFSEKYANQVNMAWMWARLHARTTRLATYQGGFQAFADEFAAILAKMGVKFAYRTVV
                     KQLMSEKEGGLTLSTTHGNETYDRALVTISPALLSKMAPQLPEDYLKGLLDLKSMGAV
                     VMTLALKHQLSREGYYWYNLPKDAGFPFLALVEHTNFLSPQKFGGNHIIYCGDYLDPA
                     HEYFQLSKEELLQRFTPSLKKINPNFETGWITGSWLVKTNYAQPVPLLNHSRHIPSIE
                     TPLKGLYFASMSQVYPWDRGTNFAVEIGRRAADLMLRA"
     gene            40471..41814
                     /gene="dnaA"
                     /locus_tag="P5516_01065"
     CDS             40471..41814
                     /gene="dnaA"
                     /locus_tag="P5516_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HRT91058.1"
                     /translation="MDHRNAWQAAVGQLQMEMSRASYETWVQPAELVRYENDLFTVGV
                     PNAYACDWLKSRLTAKLNSILSGIMECPQKVEFIVWHKDYENQPQTASTAEPLPLISP
                     AVTGSQPVVNHRYNFDNFVVGSSNRLAHAACLAVAENPAQAYNPLFLYGGVGLGKTHL
                     LHAIGNFAQARGEQVLYVSSEEFTNDLINAIRTHTTQAFRDRYRRIDVLLVDDIQFIA
                     GKESTQEEFFHTFNTLHGQDKQIVISSDRPPKAMNTLEERLRSRFEWGLTADIQPPDL
                     ETRIAILRSKAERSRHAVPPAILEIIARQFQSNIRELEGALTRVVAYADLRGRPLSEE
                     LVNVALTDLLPQRNAFEPAHVVNVVASAFGITPEKLMGRDRTRAAALPRQVVMYLLRQ
                     EANVSLPQIGEAMGGRDHTTVMYACEKVADMIERDDRLRRQVLQIREQLYGRKTT"
     gene            complement(41859..42710)
                     /locus_tag="P5516_01070"
     CDS             complement(41859..42710)
                     /locus_tag="P5516_01070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91059.1"
                     /translation="MSQTSSKTRLFLAILAVVVGVFMVGVAPFLIQTSLDRVMTALVK
                     VSAQKPAYASGILLFSVLYPLYRAIIFIAGITLIVIARSIREGKEWTFPVGLLASAFP
                     ASGGMFMMLPYVSFVPGLPLPMLVSLTGLLFFLCLILLRKVNTQKKIAQLLTLTFCGI
                     MTAHAFVIAVGGMRQTLTRLEKPLYSGIEGWVLAWSSPVQFICVILLFVAIYKIAARK
                     MEGWWMALVAAVSLVAMNTPTQIIRTLRTDATSIDYLIGALLSTALLVILLLPKIKRA
                     LFEEPAS"
     gene            43096..44763
                     /locus_tag="P5516_01075"
     CDS             43096..44763
                     /locus_tag="P5516_01075"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - ribonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HRT91060.1"
                     /translation="MNKQDKKDSHRKGALKIIPLGGLGEVGANMMVYEYDGQILVVDT
                     GLMFPENDMLGIDYIIPDISYLVQRRKQVKGIIITHGHEDHTGAIRHVLEELQAPIYA
                     TPLTRGLLEVKLARNGGASKAQIQTVNAGENRKIGPFSVDFFHVCHSIPDGVGLGIEC
                     PAGLVVHTGDYKFDHTPVDNWPTDYAKLAEFQRRGVLVLLADSTNSERQGWTPSERVI
                     DDAFDKVFREADGRILIATFASLISRMQQVGNAALKHNRKTAFAGLSMVDNAKIAREL
                     GYLQLPDNLIVSLDEALHMKDSDVVIMCTGSQGEPTSILGRLSRGTHRVFDVKNGDTI
                     VLSSHPIPGNEENVYGTINRLLERGANVIYEAIAPVHVSGHASQEEMKLLLHLTQPEY
                     FIPIHGELRQLKQHARLARQIGIPEENIAVILNGQSVELSHGKMRMAEKVPASYVYVD
                     GSGVGDVSDAVMHDRELLSEEGLMIVNLTMDKYTDRPKKEPDFITRGFIAQQDGNELM
                     TGLKARVMSVADRANGHLQQDVEQAVSNYLYRETRRKPMVLVNIIKS"
     gene            complement(44801..45457)
                     /locus_tag="P5516_01080"
     CDS             complement(44801..45457)
                     /locus_tag="P5516_01080"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HRT91061.1"
                     /translation="MPPDFAHEREQMVRHQIEKRGITNSGVLNAMRTVPRHLFVPLEM
                     REQAYADYPLPIGSDQTISQPYIVAFMTALLNLTGSEKVLEIGTGSGYQAAVLSLLAR
                     EVITVEYLPALARSAGDTLRDLGYLNVTVHQGDGSLGWVDQAPYDAIMVTASAPKAPE
                     PLLKQLNPEGRMVLPVGGRGVQWLELWQQENGIWLPEVSLPVAFVPLRGAFGWQDDHE
                     "
     gene            complement(45447..46526)
                     /gene="mutY"
                     /locus_tag="P5516_01085"
     CDS             complement(45447..46526)
                     /gene="mutY"
                     /locus_tag="P5516_01085"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HRT91062.1"
                     /translation="MKDELARLLLNWYRQNARSLPWRGHSDPYAIWVSEIMLQQTRVD
                     TVLPYYLRWMRDFPDISTLANASEHAVLLVWEGLGYYNRARCMRRAAQQMMQLHDGKI
                     PADYEQLLKLPGIGPYTAAAIASMAFGLDRAVVDGNVKRVLARILPYTLAVNTPTAES
                     ALRHAAQELLPEGNAGDFNQALMELGALVCLPRKPLCDRCPLPSICAAFQQGAQAELP
                     LMKEKKPVPHLVVTAAILRQDDRVLIAKRPARGLLGGLWEFPGGKVKENETHADALAR
                     ELMEELGVSTQVGNRFGTYRHAYTHFSITLHAYEIISQKKTAKPLHHAELRWVALDEL
                     ESYPMGKIDRMISRDLVQAELANAA"
     gene            complement(46535..46831)
                     /locus_tag="P5516_01090"
     CDS             complement(46535..46831)
                     /locus_tag="P5516_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91063.1"
                     /translation="MKLIMSWDITPEHEQDYFEFVIRDYIPGVQRMGCELTDAWATVY
                     GNKPQITVSVIIGSPRKARQLLFSEDWLSLNNRLQDFIQNYTMKLVYARNRFQL"
     gene            complement(46852..47517)
                     /locus_tag="P5516_01095"
     CDS             complement(46852..47517)
                     /locus_tag="P5516_01095"
                     /inference="COORDINATES: protein motif:HMM:NF024874.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT91064.1"
                     /translation="MEITWYGHSCFRLTERSMATVVTDPYDCKQVGYEPLKVKGDVVT
                     VSHNTPGHNLISAVKGYSHLIDGPGEFEIGGVFITGIQTNGHSRKAENEPRNTMYLFD
                     YNGINVLHLGDLNRVPSQAEVEAIGPVHVALVPVGAGGSLTAIKATEVISLLEPNIVI
                     PMHYATPHTASTPQKLEPLSRFLKEMGLSNVEPVPTLKVTGISSLPDETRVVILQYQM
                     NNS"
     gene            complement(47790..48887)
                     /locus_tag="P5516_01100"
     CDS             complement(47790..48887)
                     /locus_tag="P5516_01100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91065.1"
                     /translation="MTSVYTIQNPEAFTSPPATPTKGTGCTPSVRGLNPAKPGDTISP
                     GASYADAAGKETSVIQDRWFFNGQESSSMIWDGKPVTVELQWTCLDHSGHSKTFTIPA
                     YQGEAAAPLTQPQTGQPAETSQEAQPKRPKTVAPWGIAAIISSIVGLVGAASVGIGYA
                     LKKKPAAPAPPAPPAQSPPPNIPVQYPPIPDPAGFSKIGAPTRYTPPATAGRQFTADV
                     PVQNTPPQVEPSSHQPAGMTPAQREELLSIRGQMQIEAANLKNKYYDNKEKIDRLKGI
                     LKNNMLKFITKKGFDVQEWMLEGPTDVVNKIIVDPIMEKAFQKHDTSQDGKIIVDMYN
                     RIKQMENEMHELSKEFEYLQNEMNKIDQKLK"
     gene            complement(48954..49340)
                     /locus_tag="P5516_01105"
     CDS             complement(48954..49340)
                     /locus_tag="P5516_01105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91066.1"
                     /translation="MESRIQRTLLSFFFASFFFIFYFLMYLPNDVEAQGEVEPQRVIA
                     TLYDSDCFPNGVENRIEFFNVGSQGGEAYSKAVYVYAITHIGNHPDGGLACFVDGYAE
                     LIGTFSGGPNGTITFPKGNYQEITTC"
     gene            complement(49554..51266)
                     /locus_tag="P5516_01110"
     CDS             complement(49554..51266)
                     /locus_tag="P5516_01110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91067.1"
                     /translation="MERTVPKSATDEIDLYLRTIYSLLRSTTEVQVRTLEEVHAGMNS
                     SLHPDARKVSLDLSALIYSSLRLPSCIMRTRSIVLGQSVQLFSRHGYPEVENWLPVTA
                     RARRRSSYFDGNSTLAVFIASRSDIEDIVPTLTAFQIEWNKMHQFLQGWNPPSWEETS
                     LEGSPSFVELSHVLGTPLEDLQRLVSVWGSDFSSNLETIASRRCSFGIRLLSGSLSEY
                     WRATRLWWQNIERSAPLFSERPVYLISSNTHSIPNLLSGYALTRKEQILTFLQQQGNQ
                     ELLAEWHEIQERGNCASTENFLYYALKKYQQTAQGAAENAQMAREEQRHGITRVPPAH
                     SFDIEAQVVEINRLDPQHFDPRLLSDGENTVFLQKSNALIVNIDYPLGLGAYNVLTKI
                     AEHISEILGVYIMGKAATLNGVRGDIMIPGVVQDEHSQNTYLFQNVFTSPDVAPWLVF
                     GTVLDNQKALTVLGTFLQNASLMDVMYREGYTDIEMEAGPYLSAVYEMTRPKRHPVNE
                     IVNLQGVNFDIGILHYASDTPLSKGKNLGAGTLSYRGMDSTYAASVAILRRIFQQEKK
                     RLLG"
     assembly_gap    51425..51524
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<51525..51881)
                     /locus_tag="P5516_01115"
     CDS             complement(<51525..51881)
                     /locus_tag="P5516_01115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91068.1"
                     /translation="MNFIEGNLGILPASYTTQAVFFDLGFTLIHFQGDYPSVLLDSYY
                     SLARALKREGYLFEVDEFVRRFKNALADYYQSRDIDLIERPVETYLTRVLNTFGHIEP
                     PESVTRNALAEMYQTTE"
     gene            complement(51921..52946)
                     /locus_tag="P5516_01120"
     CDS             complement(51921..52946)
                     /locus_tag="P5516_01120"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRT91069.1"
                     /translation="MASAQNIEVLTTVPFPEAVMGQLRELSPRIKITCIPASKIDEIP
                     REVWQRSEVLYTDILLPDLNLSPNLRWIQFHYAGIDFLQGSELLKHKDLKLTSMSGAS
                     AIQEGEYILMMLLALGHHMRGLMKNQEKAEWPDDRWEKFSPRELNGSTVGLVGYGSIG
                     REVARLLRPFDVTVLAVKRDVMHPQDNGYVMDGHGDPEGDYFNRLYPIEALKSMLREC
                     DFVVVTLPLTPQTRGMIGEEEFNAMKPGAYLVHISRGGIVDEEALLRALNEKRLAGAA
                     VDVFSTEPLPADHPLWKAPRLIITPHVSGFSQAYKERAGRMFAANLKRYLRGEPLLNE
                     YLSDRHY"
     gene            complement(52957..53733)
                     /locus_tag="P5516_01125"
     CDS             complement(52957..53733)
                     /locus_tag="P5516_01125"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT91070.1"
                     /translation="MSDLTYTASELMCINAARLLRDGDVVFVGVGLPNLACNLARRTH
                     APNLQLIYEAGVIGARPSRLPLSIGDPTLVTGAVSVCGMFDIFSQYLQRGNIDVGFLG
                     GAQIDRFGNINATIIGGDYQHPKVRLPGSGGSQEIAGWANRCYIITPHQKRRFPEKVD
                     FITSAGFLNGRAQRELTGARGGGPLVVVTNLGLLEPDANGELVLSALHPGCTLEQVRE
                     NTGWQVRTSGQVSTTEPPLPSELQLLREELDPGGIYLKGS"
     gene            complement(53751..54653)
                     /locus_tag="P5516_01130"
     CDS             complement(53751..54653)
                     /locus_tag="P5516_01130"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT91071.1"
                     /translation="MSKICSLAEAISSYVHDGDTVFCAGFTHLIPFAAGHEIIRQGKK
                     NLTLARATPDLIYDQMVAAGCAKKLIFSYIGNPGVGSLRIVREELEAGRLEWEEYSHF
                     GMISRLQAGAAGLPFMPMNQTAAPDLERSNPNIRRVKDPYTGQDVVTVPALNPDVGII
                     HVQRADEHGNAQVWGILGEQKEAAFASRDVIITAEEIVSEDVIRSDPNRTLIPGLIVK
                     AVCQVPHCAHPSYTQGYYDRDNVFYLNWDKISEDRATVNAWLDEWVHGVQGAEEYWQK
                     LGSETHARLAVKPRYAAPVNYGDY"
     gene            complement(54662..56245)
                     /locus_tag="P5516_01135"
     CDS             complement(54662..56245)
                     /locus_tag="P5516_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRT91072.1"
                     /translation="MAADQVFKLTYATMFNPPEELHTRYDQALAKVKSELGRDHGMLI
                     DGKEVFAEEKFDNRSPANTDVLLGTFQKGNAEHAKAALAAARKAARGWAHTPWQERVF
                     LLRKAAAIMDRRTFEMGVVLSLEVGKNRMEGLGDVAETADLIRYACDRMEASDGFVAP
                     MGRDPLSGYESRNVSVLKPYGVWLVISPFNFPGALTGGPSGAALVAGNTLVMKPATDT
                     PWTARLIAECFQEAGLPEGVFNYVTGPGSTLGQALIESPEVDGITFTGSYDVGMKIFR
                     DFAQGRYVRPAILELGGKNPAIVTKNADLEAAAIGIVRSAFGLQGQKCSANSRVLIHS
                     SVYDQLVARLVELTGKLTIGDPTERANYLGPVVNKGSYRDYQGFVGELAAGGKILTGG
                     KVLTDGDLAKGYFCAPTIAEAPYTHRLWKYEMFVPITTVGKFDDLETAMKIANDVEYG
                     LTAGFYGTEEEAAWFFDNIEAGVTYANRPMGSTTGAWPGFQPFGGWKGSGSSGKNAGG
                     LHYLQLYMHEQIQNLVRKV"
     gene            56492..57439
                     /locus_tag="P5516_01140"
     CDS             56492..57439
                     /locus_tag="P5516_01140"
                     /inference="COORDINATES: protein motif:HMM:NF039905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRT91073.1"
                     /translation="MIPAAQPVKTAFVVINPVAGIVNAALLQRNIESRFKHKGWQTRF
                     HLTAAGEDLKTIVQQQILSGVDLVVAAGGDGTVAAVAAGMVHAPVPLGIIPTGTWNAI
                     ARNLMIPFSSTRAVNLMTGRHRIKQLDLMAIGETVHAMNLGIGFSSMMIENTARAEKR
                     KFGSLAYFSQGFKQIFGLQQRRYMIEVDGKRYRGRAAEIFVANYGMVGLNVLEASLDI
                     KPDDGKVDVLILRARNLFDLPGMFWQMFVQRKRRTPKYRQLSAAESLFIRTNPPAAVQ
                     ADGELIGKTPVTVQVLPRCVKVIVPLEPPLSFPLQPRRV"
     gene            complement(57421..58152)
                     /locus_tag="P5516_01145"
     CDS             complement(57421..58152)
                     /locus_tag="P5516_01145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91074.1"
                     /translation="MPAADRFEILLLIARPAAGKSEIIDYLKKTTLRQRLQRFHIGKL
                     EEIDDFPMLWTWFEEDALLERMGYPRLHTDSAGYFLGNHLWNLLIERICLEYQKRLRD
                     VPGLAREYTTLIEFSRGSEHGGYRSAFCHLSPDMLERMAVLYVDVSWEESLRKNRLRF
                     NPEKPDSILEHGIPDDKLERLYRQSDWEELSAADPEYLEIQGRRVPYAVFDNSDDVTT
                     ARGTTLGERLREVTFLLWERYTRRG"
     gene            58270..59709
                     /locus_tag="P5516_01150"
     CDS             58270..59709
                     /locus_tag="P5516_01150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT91075.1"
                     /translation="MIESETTHRNKWLILLAIGMGVFLATIDGGIVNIGLNTLVKDFG
                     TPLAVTEWVVLAYMLTISSLMLSIGRLGDMIGKKYLYIAGLAIFTVGSVLCGMSETIY
                     WLIAFRVLQAVGASITMALGTAMITEAFPDHQRGKALGIMGTLVSIGIIAGPTIGGLI
                     LEHLTWHWLFFVNLPVGILGVLMVWHYVPLDRPGKKQSFDSLGAGLLFITLSSLLLAL
                     SRIQSAGLTDPLIPGLLFLFLIGLIVFIRVEKRSAEPMIDLSMFRNRMFSVNLITGFL
                     TFVVGAGTMILFPLYLQNVLGFGPQQSGLMMAVTPLTVAIVAPFSGALSDRVSSRVLT
                     SIGLGVMLVGHSLISTLSTDTSVTGYLLRFIPLGIGIGLFQAPNNNAIMGSAARERSG
                     VASSLLSLTRTVGQTTGIALLGAFWERRATTLAGDDALHSALAAPLKAQVGGLQQTMY
                     LVIAVIIAALGLSLWALAAWLRERSPQNA"
     gene            59793..60926
                     /locus_tag="P5516_01155"
     CDS             59793..60926
                     /locus_tag="P5516_01155"
                     /inference="COORDINATES: protein motif:HMM:NF012618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91076.1"
                     /translation="MMRKYLIWITTIFMLASFMLVSCSLPEVTPPAAGETAPVVSTPS
                     EQTVPLPAQVITATPAQTRLIKASEVGRLEISQRVAVSNIQDISWSPDSNTLALVTQN
                     TDAAQNQVFSVTLLNAKDLSTQAVYSATGNRVTSVAADGKTAAVINQDQMSFSLVDTS
                     VKDGASRSRVTDFLIGNVTISPDMRYVAVTKAEFWEVILYDFNTLEEVRFLSGFSTAA
                     PVFDAGFNHSPQWLVWHARATLQLQEVETGMLSNLYSHEDFVTAYTLSPDGMILASAS
                     FRSTNDASVPVVILWDTLNGTELKVLEISQPVNALQFSPDGKLLAVAAGSDLQIWDAA
                     GGNLLTTLTGHSDFILQLAFSPDQHTIASAGQDKQLYLWQILE"
     gene            61028..62935
                     /locus_tag="P5516_01160"
     CDS             61028..62935
                     /locus_tag="P5516_01160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HRT91077.1"
                     /translation="MPLLTAANLSKSYGALDIFPEISFSIPHRARIGLVGANGVGKTT
                     LLRVLMGEEEPSSGTIHKARGIRIGYLPQEAALDSSRTLWGECMTVFEPLLALQQELH
                     AMEEALSANPDSTDLMQVYGERQREFEHLGGYEFEARTRITLSGLGFKRADEKRPVRQ
                     LSGGQRTRVLLAKLLLSEPELLLLDEPTNHLDISAVEWLEGFFKEWQGAVLIVSHDRY
                     FLDQTTTTIWEMTPALEEYRGNYSAYLAQREERYQRRLQEYQAQQEFIEKEEEYIRRN
                     IAGQNTRQAQGRRTRLERLLSEARLTPPLQRRTLRMQLVSSGRSGDLVLRTRSLSVGY
                     ADEGKPLFEVDDLTLVRGECAAIIGPNGAGKTTFLKTILEQIPPYAGETVLGASLKVG
                     YFAQAHEGLHPDWTLMDEIQAMMPKWLPGEIRDYLAKFLFTGEDVFKTVHLLSGGERG
                     RLALACLALQGANLLLLDEPTNHLDLPSQEILQNILGQFQGTILLVSHDRYLIDALAT
                     QVWEVLPEQRKLAVFEGSYSEYRALKQANLMIPDGRPQQKVGLTRAKKPAVGKSKDQL
                     QRMKARLRALEEEIARLEIDARGVEQRLSDRGVDPGEVQQLASEYARLQAQLEAKIEL
                     WSDLGDELSEP"
     gene            complement(62983..63804)
                     /locus_tag="P5516_01165"
     CDS             complement(62983..63804)
                     /locus_tag="P5516_01165"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HRT91078.1"
                     /translation="MLDLTIDTNLARKILTDFIHTEITRAGFSRAVLGLSGGLDSALA
                     CYLAAEALQPENVLAVRMPYATSSADSLAHAQLVIDALGVRTLTIPITDMVEPLFNRY
                     PDMGNRRRGNVMARQRMIILFDQSEVFKGLVLGTSNKTEILLGYTTMHGDAACALNPL
                     GDLYKTQLRQLAAAVGVPEPLLQKPPSADLWAGQTDEDELGFTYAAVDQLLYLLVDQR
                     YTPQECVDSGFEVKFVHAVIERVRKNQFKRVMPPIAKLSGRTVGYDFLYLRDWGT"
     gene            complement(63825..64703)
                     /locus_tag="P5516_01170"
     CDS             complement(63825..64703)
                     /locus_tag="P5516_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRT91079.1"
                     /translation="MKLNVAMAQINTVLGNVEQNLEKHLDLIEQARRAGADLITFPEL
                     SLTGYALQDLTPTVACRPLPDDPVFNPLLVASQDIDIQVGFVEEDERNLFYIATAFLS
                     GGAVRHIHHKVYLPTYGFFDEGRFFARGESVRAFDTRFGRVGMLICEDFWHASSPYML
                     WLDGADLFLFTSASPGRGITPTPHNTAPGRTESARWVNDIIRAYAGMFTTFVVHTNRA
                     GFEDGIHFWGGSSAFSPDGEPLVNGVEHAEALVLAELDLSQLRRTRTRLPLLRDERTS
                     LVQREITRIITRNADG"
     gene            complement(64720..65454)
                     /locus_tag="P5516_01175"
     CDS             complement(64720..65454)
                     /locus_tag="P5516_01175"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRT91080.1"
                     /translation="MLTLRLFSVLAGFGAAIGLWRVYRSIPAGVESRQWICAVFALLG
                     VLTGARVGYVLAHPFYYSLHPEQAGQFWLGGFNAFGAFAGAVFFALLGSAALRLNVLS
                     ALDSTSRMLLPTAALIWLGLWYEGVAYGQTLPSGAFLSYPALDETGMLSNRFPLQLVA
                     ALSLLLLLGLIEHITQFKRPGFRFSLVSLVFFTHTLVFSLFRADVVFPIRGIRSDGVL
                     SLIFAVAFALLSVLLLLTSDQKKRIK"
     gene            complement(65504..65758)
                     /locus_tag="P5516_01180"
     CDS             complement(65504..65758)
                     /locus_tag="P5516_01180"
                     /inference="COORDINATES: protein motif:HMM:NF013286.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HRT91081.1"
                     /translation="MMSDNCCSYSELELLRGLIEQLSAYINTYHGGEVEFVSFDGKVV
                     KVRMGGACVGCPHSPATIKGWVEGTVRQFFPEITGVEAIE"
     gene            complement(65758..66243)
                     /locus_tag="P5516_01185"
     CDS             complement(65758..66243)
                     /locus_tag="P5516_01185"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRT91082.1"
                     /translation="MLNGSPLPVQIFEGFSLDEQDELRSIIEVFTYPAGETILRQSQQ
                     AVHLFILVTGEVEITHVPYDGPELTIGKLTSGGVFGWSSILGRKTYSSSVFATTDSRV
                     YRIAAYKLQRFCEQHQESGMVLLEKIAMSVAQQPAKAHDQIMHMLRAVIACPEGKNPE
                     E"
     gene            complement(66616..67428)
                     /locus_tag="P5516_01190"
     CDS             complement(66616..67428)
                     /locus_tag="P5516_01190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91083.1"
                     /translation="MLEVNALSAGYYHELVLEDISLQLPQGSVLALIGPNGSGKTTFI
                     RAISGVLPLRAGSVKVAGQDIYSLSPQQRARIIAVVPQARSIPPAFSAEEVVALGRTP
                     HLNWLGKVNGKDMLIIEEALEKMDLLALRSRAVSELSGGEQQRLFLARALAQESPLLL
                     LDEPTTHLDLQYQVSLMQRIHQLAHPTKEELVKGMQPKTVVVAIHDLNLVSRFADQVG
                     LLVGGKLAALGKPEEVLNAGLLSVAYNLPLEVLKEHGYTLVIPAHTPARNNS"
     gene            complement(67434..68468)
                     /locus_tag="P5516_01195"
     CDS             complement(67434..68468)
                     /locus_tag="P5516_01195"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRT91084.1"
                     /translation="MRKKNTRSPFLVLALALAASALLSVAVGSVFIPLPTLWDVVTSA
                     LRGATSTLDVNGVYLKIISDLRLPRMLMLTMVGAALAGSGAAYQGLFRNPLADPFLIG
                     VSSGAGLGAVLAMLARSPYTRGGLLSVPLAAFCGALLTVLIVTLLARVGKTVPTTNLI
                     LAGVAVSSFTSAVTSALMIHSTGELRRALVWLMGGAIMTGWSPILAVLPYMLIALAGL
                     VLIGHPLNVLQFGEEQALQLGIRVQRARWITIALATLSTAAAVAFAGIIGFVGLVVPH
                     IIRLLWGTDYRRLIPLSMLGGASLLLLTDVVARSLIAPQEIPVGIVTALLGAPFFLWV
                     LKLAKAQNYW"
     gene            complement(68468..69391)
                     /locus_tag="P5516_01200"
     CDS             complement(68468..69391)
                     /locus_tag="P5516_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-binding protein"
                     /protein_id="HRT91085.1"
                     /translation="MKHARIIRIAVFLLLLMLAACSAIPAAKDGGKIQLQDGLGREVL
                     LEKPAARIISLSPSVTEILFAVGAGSQVVGRDTFSNYPEEVTSIQDVGGSMGSYSLET
                     IASLNPDLVIAAEINTPEQVSALEGLGVKVYYLSNPTDLEGIYGLLQTVGALSGHTKE
                     AAALTSSLKERVQLVQQAIKKAETKPVVFYELDGTDPAKPWTPGPGSFLDLLINEAGG
                     RNAAAAMQSQWAQISIEELLVQNPDIILLGDSIYGMTPEQVAARPGWDGLRAVQEGRV
                     YPFNDDLVSRPGPRLVDGLEELARLVHPELY"
     regulatory      complement(69496..69635)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            69758..70288
                     /locus_tag="P5516_01205"
     CDS             69758..70288
                     /locus_tag="P5516_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HRT91086.1"
                     /translation="MTRVPYEIRVGELLKEKQKKLVLAESCTGGLVGNLITNIAGSSD
                     YYLGSVTAYAYEAKEALLGVRHETLLQYGAVSRQTVLEMAAGARRSLAGGIPLDSIIG
                     VSISGIAGPGGGLPDKPVGTVWFGMSTADGDWAWRCMFKGNRVQNKRSAARTALKLVI
                     AYLAGSLPPETRSQRH"
     gene            70298..71509
                     /locus_tag="P5516_01210"
     CDS             70298..71509
                     /locus_tag="P5516_01210"
                     /inference="COORDINATES: protein motif:HMM:NF018613.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT91087.1"
                     /translation="MAKRLIRILILTADAGFGHRSAANAVAKALKLKYQSAVEVRVEN
                     PLDLESAPFFLREAQTDYDRWVKQVPELYKLGYQASDAAIPTRLLEDSLAVLLFEAIR
                     DTFSGPLPDVVLTTYPMYQSAIMLLYRLRKIHIPLFTVVTDLSTVHRLWFNRNVDGCL
                     VPNEIVANLAAVNGVPQDKVHITGIPVSPDILYETRPAEKIRAELGWHPELTTILAVG
                     STRTEKLLEAASVINHYGSELQLVVVAGKNEELYQELNQIGWHIPVQLFDFVDKVPLL
                     MKASDLVICKAGGLIVTEALACGLPTVLTDIIPGQETGNAELIQRLQAGVVAETPLMM
                     MEALHHFLMNDQKMLRQYADNARTIGKPAAACDVAEILYEAGKASTPPKASYRARGFR
                     PAERPLEGDSE"
     gene            71506..>72160
                     /locus_tag="P5516_01215"
     CDS             71506..>72160
                     /locus_tag="P5516_01215"
                     /inference="COORDINATES: protein motif:HMM:NF013232.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/S-adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HRT91088.1"
                     /translation="MKENGEVEVAILLSALSEWQTAQSLFSDTGNENSPFGDYFLTCV
                     AEKRCVFFHGGWGKISAAASTQFVIDRWQPKLLVNLGTCGGFEGHTAVGEVVLVDETM
                     VYDIHERMNDPFEALQFYTTRIDLSFLRRPYPQAVRIGRLVSADEDIDPAKINRLRQE
                     FGAVVADWESAAIAWTAQRNGVRVLILRAVSDLVSESGGELYNPKRAFPRSLLRKIVR
                     "
BASE COUNT        16379 a        18725 c        20416 g        16340 t
ORIGIN      
        1 cacatcgacg acttcgctcg gcacctcgat gtcggctcat cgcatcctgg ggctggataa
       61 ggtcccaagg gtcgggctgt tcgcccgtta aagcggtacg cgagctgggt tcagaccgtc
      121 gtgagacagg tcggtctcta tccgctgtgg gcgtaaggga tttgagtgga gctgccccta
      181 gtacgagagg accggggtgg actgaccgat ggtgttccag ttgtcgtgcc aacggcatcg
      241 ctgggtagct aagtcaggca gagataaccg ctgaatgcat ctaagtggga aactcgccac
      301 aagattagat ccctgttttc ccgtaaggga gtaagatcac aggtagacta cctgtttgat
      361 aggcagaatg tgtaagcgca gcaatgcgtt gagctaatct gtactaatga tcgagggctt
      421 cttcatatgg tgcggggaac tcggcactct tttatggact acatactatc gtaaggttgg
      481 tatcttaact tcgtgaatta agtctcttgg tgactggagc gacagtggta cacccggtaa
      541 catcccgaac ccggtcgtta aggctgtcag cgccgatggt acttgggggg cgactccctg
      601 ggagagtagg tcgttgccaa gagacttttt tctattaacg ccaatccacc gtggagcgct
      661 gtcgcggtgg tttttttgtt ctctggcggc tctccatgat aaaaaaaaaa taaaaaacca
      721 aaaaaaacct ccggaagagt ggactcccgg aggttttttt tccactcgtt ataaaatacg
      781 ctgcaaatgc tggctataac gtccttcgtt cccgatcgta gttctgaccc aggaaagccg
      841 gaacgcgcga cttaccgaag gtgaagatca tcacgatgat cgtggcgatg tacggcagca
      901 ttgaaaggaa ctggtagggg atgaaggaac cgcctacctg gaagcggaac gacagcacct
      961 cgatggcggc gaagaacagc gagccgatga ggatgggcag cgggctccag gcgccgaaga
     1021 aggtcagcgc gatggcgatc cagccgcgac ccttggtcat caagtcagtg aaagtgccgg
     1081 tgtataccat gggaaggtaa gcgcccgcca ggccgatgag catcccgccg atgatggagg
     1141 tgatgtagcg gttggaaacc acgttgatac ccagcgcgtc tgaggctttg gggttttcac
     1201 ctaccgaacg caggcgcaag cccatgttgg tacggaacaa tatgtaccac aggaatatcg
     1261 aaagcaggat cgagaaatag accaggatgt tctgcttgaa gagcaccggc ccgatgatgg
     1321 ggatctggct gagcaggggg atggggattt cgggcagtgt ctttaccagc ggcggggtga
     1381 gagtgatgcc gatgacgagc ttgggtaaaa gagaagataa cccggtacca ataaagaaca
     1441 gcgccaagcc cacgacaaac tggtccatct ttaacgtggt ggtgaagtat gccaaaacca
     1501 agccaaagag gccgcctgcc gccatagcca ccagcgcacc gattaggtta ctgccagtgg
     1561 tgtaactcac cagaaagccc acagaagcgc caacaagcat gattccttcc tgcgccacat
     1621 tgaacacccc ggtacgaccg ccgagcattg tgccctggct tgccagtaca aatgggacca
     1681 tggacaggaa agtgagtttg aggaaatcag tgatggttgt ttctaaaccg ctcatttctg
     1741 cctcctcttg atgatgtcag agagaagaat aaagatcagg atcaacgctt ctgcgatcag
     1801 cacgatctgc accggcacca tgatggtacg ctgcatggcg ctgccgccta cttcaagaat
     1861 ggaaataaag aatgccacga agggaactgc ctggttcttg ccgcgcgcca tcaggccgat
     1921 gatcagcccg agggtcggga agttggagtg caacccctcg atgaggcggt ggtgggtgcc
     1981 ggccgcttcg atgacccccg ccaatccagc aacggccgcc ccgatgacca gtgaaaacag
     2041 gaacatcttt cgcgtatcaa tgccgtgcac tttggaagca cgcgggttgg cccctgttac
     2101 cagcaattca taaccagaag tcgttttact ggtgaagatg tacaccgcaa tcgccacgat
     2161 gacggccagg atgatgccgg aatgcagcgg agggtttttc accagcatag gtaaaaagcc
     2221 gttcgcgcca atcggtatcg tggtggggtg cccagccgcg ggatcgcgcc agggaccggt
     2281 ggctacaaag tcgaccagcg caaacgaaat gaagttcaac agcacggtcg agagaatctc
     2341 gttgatgttg aactggtaga gcagccaggc tgcgatcaaa ccccagaaag aaccagccag
     2401 catacccgcg aggaccacca gcggcagcaa caacgccggg ggcaggtctt taaaaagaat
     2461 gccaaccgcg gtagcgccga tggcgccaac cagcatctgt ccttcggctc cgatattgaa
     2521 cttacctgat gataagggaa cggtaaaagc cagcgcggtg agcgtcagcg ggatccactt
     2581 taaaagggtt tggacaaacc cgttgggcgt gcggaaggag gtgaagagaa tggtctgata
     2641 ggcttttatc gggttaaaac ccagggccca taacagtacc cctacaacca aaaaagccgc
     2701 caggacagag accaggtaag gaaagaattt tttaatttgt gtcatagtat acgctctctc
     2761 cctttattta ctttcctgct cgtaaccgct catcaggcga ccaatctcgt acttctcgaa
     2821 cttctcacgg gggagaatcc ctactacttt accgcggtac ataacggcga tgcggttgca
     2881 aatgagcagc agttcatcga ggtcttcaga aaggagcagg attgctgtgc cggtggcgga
     2941 atttccgagg agcttctggt agacaaaatc catggaagga atatccaggc cccgggtggg
     3001 attgtgggcg accatcaacc tggagggctg cgagaaggcc ctggcgatca tgacgcgctg
     3061 gatattgccc cctgataagt taccgcctat ttcatccggt ccagtggtct tgatgttgta
     3121 ctcgctgatc atcttcctgg aattctcatg cacgttcttc cagttgatga tccctttttt
     3181 gctatacggt tcaaggttgt gatagcccaa aatgaggtta tgcgcaacgc tggctttgtt
     3241 taagaaccca tcagaaaggc gatcttcagg gacatatacg aaaccttcag cgatacgttc
     3301 tttggtactg tgagtggtga tctcttgacc attgaagttg attttaccgc tggtaaccgg
     3361 ctgaaccccc atgaccactt cagccagttc acgctgaccg ttaccagcca cgcccgccac
     3421 tccgaaaatt tctccagaac cgatgctgaa ggagatgtcg gtcaactgct ggatcttgtg
     3481 ctgactgatc agagaaacgc cttccaggct cagcacttct tgactgccgg caacagttgc
     3541 gctgtcctcg tgtgaggtga acatcacgct tttttccagg tccatcttat cgccgaccat
     3601 ggcacgcacc agcgcttcat cgctggcttc ttttcgcgtc aggctcacca catttttccc
     3661 ctcgcgcagc acggtgatac ggtcacaaat tgacagcacc tcgcgcagtt tgtgggtgat
     3721 gaacacgatg ctcaaccctt cgcgcaccat gatttttaag gtttcaaaga gagagtcgac
     3781 ctcctgggga atgaggttgg tggtgggctc atcgaggatc aggatgttaa cgccgcggta
     3841 cagcgctttt aagatttcaa ctttctggcg ggttcccata ggcagggttt caacacgcgc
     3901 cgaaaggtca atttcaaggc caaaacgttt acaaagttcc ttgatcgccg cttcttctac
     3961 ctggtaattg gcgcttaaag gatgggagac tttggaaccc aggatgatgt tctcaagcac
     4021 tgtgtaatta ttgatctgta aaaaatgttg gtgcaccatg ccgattcctt ggcggatggc
     4081 gtctctgggt gagtgtatgg ttactggttg ccctttcagc agaattgtcc cggaatccgc
     4141 acggtaaaga ccataaagaa tattcatcaa ggtggtcttt cctgcgccgt ttccgcccag
     4201 taaacccagg atctctttcg gttttaattc aaaatcgaca ttacacaggg caaccaccct
     4261 atcaaaggat ttgttgacct gttgcatctg aagcacattt tcttgttcca tattcccgtc
     4321 ctgttcagag ggttttagca acttccgggg gagcttcaag catgatgttt cggctcgacc
     4381 ccggaagtta tcgtctcaag ttatttcaac aagagttatt tggcggaggt aacttcggta
     4441 gaatccttta caaccacgat ttctccgcta atgactttgt tgatgacctc ttcaaccttg
     4501 gcggcgacct cgggggtcac gttctgcagc gggagctgca gcgctacacc ggtgtcgaac
     4561 cctaacgggt agtaaccacc cgttttaccg ccttcaatgc tggtgatgat atcaaccagt
     4621 ggcttggaga agtcgtacag cacggcagtg atatagttat cggggccgaa gctggtttta
     4681 tcggagtatt tggcggtgat gaggatcttc ttgtcagtag tggctttggc ggcttcgaaa
     4741 acacccacca tgcccaggtt cacagaaccc atgatgacgt caacgccgtc agcgatgagc
     4801 gcgtcagcca gctggcgagt cttggccggg tcattgaagt cagccgtcca cacaggaacg
     4861 agctcaacct tgatatccag gtcgctgata gcctgctgga tggcgtgtac ttcctggtag
     4921 gagaagggaa gggcctgccc gccgaggtag gcgatctttc cggtctgtga ggccagggct
     4981 gcagcggcgc cgataccgta gaagccaaca ttgaagttgc ggtcaatgaa ccacaggttc
     5041 ggtgaagggt cagcgatggg tgcgtcacct tcggcaatga acacaacctc ggggaagagc
     5101 ttggcgagtt cagcggtctg gttcacaaac tgaccaccgt gggtccaaat gacgttgaac
     5161 cccgcgtcaa tgtattcgcg cattacacgg tcaacatcag gcacaggaac gctttcggag
     5221 tataccgcct ggattccgag ttgttcctgc gcagcggtca tgcccacata agccaaggtg
     5281 ttgtaatcgt tatccgtaat cacacccggg aagatagcag ccagcttgac ctccatggct
     5341 ggttcctgcg ccgcgggggc ttcggtagcc gctggggctt cggtctcttg aactgcgggg
     5401 ggttctgtgg caactggcgc cgcggtgggc ttagcgcatg cagagagcaa tacgctcaaa
     5461 accagcaaaa cagagatggc aaacaacagg ttctttttca tttcttctcc tttttgatat
     5521 tggtgggatt ttctcccact ggcatcaagc cgcttacatt ttacccgaat tacgccgttt
     5581 ttattcctgc ttaatttccc gatatctcaa tgacatatac cctgtttaaa aacagcgagg
     5641 gagaaatttg ctcattcaaa ataacctgtt tttcgtccct gtgcgccaaa taatgaaagg
     5701 gagtaacctg cccaatgtcc ataactgcct gtaaacgacg ggcaaaaaaa gattgacaga
     5761 gaccttgcac tcccgttata attacgcttg cagttggtta gaaaaaccaa agaactttca
     5821 aagaggtgat gaccgaaaac tcgatacatc cgcgaaagaa ttctggagga aggacactga
     5881 tcgactgcgt gactttacag tcgattttat tttcccggcg aagggaggtg agaaaccatg
     5941 gcttctgttg ttttgcgtcc caatgaatca caggattcac tgctgaaacg cttccgcaag
     6001 aaggtagtga agagcggcgt tttaagcacc attcgccgca agcgctggtt tgtttccaag
     6061 agcgaacttc gccgcgtaga aaagaaaaag gccatcaggc gtatcaagcg ccgccagtat
     6121 acacgggtgg gcgaatagcc tacttgacga acgagcagcc cgagaggctg ggataggagg
     6181 aattatggcc aaagaagaag ataagataca gatagaaggc gagatcatcg aagcgctgcc
     6241 cggaacccag ttccgcgtac gcctcgagaa cggccatgag gtactcgctt atctttccgg
     6301 gcggatgcgc aggtattaca tccgcatttt gctgggcgat cgcgttaaag tggagatgag
     6361 cccgtacgac ctgacccgcg gacgcatcgt ttaccggcag aagaagaacg aacccatact
     6421 ggatgaacca accagataaa aaagaccggt ctttgaataa aagaccggtc tttttgtttg
     6481 ggcgattatt gctctttttc ctcacccggt ttgctgtagg ccatggcagc catttgcttg
     6541 tacaggtccc atttacgttg cgcgaattgc gccgcttcgt tcatgagcat ttcagcgcga
     6601 ttctcatcgc tttgtaccag catgcggtaa cgggtttcgt tataggcgta atcctttacc
     6661 gccacggtgg gctcgcggct gtcgagctgc agcgggttct ttccttcgtt caccagggtg
     6721 gggttgtagc ggaacagcag ccacaaacca gtagcggcaa gcagtttttg ctgttcgagg
     6781 cccttctgca tatcaatgcc gtgcgcgata cagtggctgt aagcgataat gagggcaggt
     6841 ccgtcatagg catctgcctc gaggaaggct ttgagggtct ggttgtcatt ggcgcccatc
     6901 gccacgttag cgacgtaaac gtaaccgtaa gacatggcca tcaaccccaa gtcttttttg
     6961 ccaagaccct tgcctttggc ggagaatttt gccaccgccg ccaatggcgt ggcttttgag
     7021 gattgaccgc cagtgttgga gtagacttct gtatccagca ccaggatgtt gacgttgcgc
     7081 cctgaagaaa gcacatggtc aagaccacca tagccaatat cgtaagccca accgtcaccg
     7141 ccaatgatcc acacgctctt ctttaccagc gcgtctgcca cacccagcag catcgaagcg
     7201 ttggcatctt tactggtcgc cagtttttct tttaatgctt ccacgcgttt gcgctgctgg
     7261 cggacgccag tttcagtgga ttggtcagcg ttaaggattt catccaccag ggttgtaccc
     7321 agctcgcctg ccattttagc gagcagctca cgcgcgaagg cgttctgctg gtccagcgtc
     7381 aggcgcatac cgagaccgaa ctcggcgttg tcttcaaaca acgagtttgc ccaggcgggt
     7441 ccgcgcccct ggttgtttac cgcccagggc gtggtgggca ggttagcgcc gtagatggaa
     7501 gaacagccgg tggcgttggc aacgatggta cggtcaccaa acaattgggt gaccaattta
     7561 atgtagggcg tttcaccgca accggcgcag gcgccggaga actcaaacag gggttcgacc
     7621 agttgcgagt tcttgaccgt tgtgggttgg taaaggtcgg ggtcaggtgc tgggatggag
     7681 aggaagtagt cccagtttat ggcttcctgt tcacgcagag ggagttgggg cgccatgttg
     7741 atggctttac gcagcgggtt gttcttatct cgcgccgggc aggcctcaac acacaggctg
     7801 caacctgtgc aatcttctgg cgccacctgg atggtgtact tcatgccggg gaattctttg
     7861 aacttggctt cgatggactt gaaggttggc ggcgcgtctt tgaggtaaga ggggtcatac
     7921 accttttggc ggatgacggc gtgcgggcaa acgaaagcgc acttaccgca ctgaatgcac
     7981 aggtcggttt cccacacggg gatctcaaga gcgaggttnn nnnnnnnnnn nnnnnnnnnn
     8041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8101 nnnnnnnnnn nnnnnnnntc gttggcgttg atcatgtaga atttgagctt cttctcaata
     8161 atctcctgct gcacctcgat gggcagcata tcccatactt catcgcggtc atagacgctg
     8221 ttgagcagga agatggcgcc aggcgcggcg tatttgagca tatccagcca ttccaaaaaa
     8281 ctaaactggt ggcaggcaac aaattgggcg tcttcctttt caatgaggta aggagcgcga
     8341 atctggtgag gcccaaagcg caggtgagag atggtaaccg agccagattt tttcgaatca
     8401 tagtagaaga atccctgcgc gtagttattg gtctcttcgc cgatgatctt gatggagttc
     8461 ttgttggcgc cgacggtccc gtcagaaccc aggccgaaga acacgcagcg gatggtcttt
     8521 tcgtgagcga tgcagaactt gtggtcgtaa tccaggctgg tatgagtgac gtcatcgaca
     8581 atccccacgg tgaagtggtt cttaggctca gctttgacca tttcgttgaa aacgccgttc
     8641 accatggctg gggtgaactc tttggaggag agcccgtagc gcccgccaat gatgcgcggg
     8701 gcgactttga aaggagtttt gccggtggag agggcttcat taacagcggt gaccacatcc
     8761 tggtagagcg gttcgccaat ggcgccgggt tccttggttc ggtcgagcac ggcgatggtc
     8821 ttgactgtgg cgggcagcgc ggaaacaaaa tgcgccacgg agaacgggcg gtataagcgc
     8881 acgttgacca cgccaacctt acgaccctgc tgggtaagga acttcactgt ctcttcagcg
     8941 gtttcagcac ctgaacccat aaggaccagc acctcggtgg cgtctggcgc gccgacataa
     9001 tcaaagaggt ggtaacttcg cccagtgagt ttggcaaatt tatccatggc attctgaacg
     9061 atgtcaggcg tgcgcgcgta aaaacggttg gctgtttcgc gcccctggaa gaaaacatca
     9121 gggttctggg ctgtaccgcg aatgaccggg cgctcgggtg aaagcgcgcg cgcgcgaaca
     9181 gcggatatga gtttttcatc gatcatggct ttgaggtcgt cttcgcacag gcgctcgatc
     9241 ttgttgatct cagcggatgt gcggaagccg tcaaagaaat gcacaaaagg cacacgcgct
     9301 tcgagggtgg cggcgttggc gatcaacgcc tggtcctgcg cggtctgcac agaatctgaa
     9361 gccaggaagg caaagcctgt ctggcgggcg gccattacat cagaatgatc gccatagatt
     9421 gaaagcgcgt gcgtggcgac cgtgcgcgct gccacatgga atacagtgga ggtgagctcg
     9481 ccggcgattt tgtacatgtt ggggatcatc aacaacagac cctgcgaagc ggtaaaggtg
     9541 gtgctcaggg cgccggcttg cagcgcgccg tgcaccgcgc ctgccgcgcc gccctctgat
     9601 tgcatttcta tcacagtcgg tactgtaccc cacaggttac gcactcctgc agccatccag
     9661 gcgtcggaga attctcccat gctcgaggag ggcgtgatag gataaatcgc gatgacttcc
     9721 gccaggcggt aggcaatatt cgccaccgct tcgttggcgt ctatagtgat caattctctt
     9781 ttcattttca actccagtcg gttggttata ggcgttcagc cttaatcgtc agcgttgccg
     9841 ccaagattat aatacagaaa cactccgatt tcccccgttc aaccgccatc gggttgaatc
     9901 gagtaacaag ctacacgaca aatctcagga cagcagagga tgcgcgagca tgagaaccgc
     9961 cagggtttta ctgtcctgta tctctccacg tctgaccata gcgagcgcat ccggcagcga
    10021 tactttgatg atattaagga actcgtccgc atcaggcgca agcgggtcag ggacaagatc
    10081 ttgcgccaga aaaccataca tatattcact ggcgtaacct ggcgaaaggt agaagttgcc
    10141 gagagcccgc cacgagctgg ctgacatccc aatttcttcg cgcagttcac gctgcgcagt
    10201 ttgcagcggg tcttcattat tctcgatctt accggcaggc agctccagca gttcagaccg
    10261 ggcgccaatg cggtattgtt taacaaagtg aatatacccc tcgctgtcca gcggaaggat
    10321 ggtgactgcg ttctgaattt cgatgaggtc aagaacacgc ggcttgccat caggcaggga
    10381 taggtcgacg cgtttgacag tgaacaaagg ggtgcgatgt tcgatggatt cgtgatggat
    10441 ggtataggtc atcggattgg acctcgcaag aaggatcacc tgggtatggt gtgggtgacc
    10501 gcgcttaatt attatggcac agaataagaa aaaggctttc ctgaaaccag gaaagccttg
    10561 tggatcacca gatggggaaa tcagggaata tgcagcacct gaccgggttt caaagaagaa
    10621 cccacttcca ggccattggc gttatagatg gcttcagggc tgacatctcc aaaagtacag
    10681 gcgatttttc cgatggaatc accggcggca accgtgtaat ctacaggatg ggcgcgcagc
    10741 gcgcgaggtc catcaaaagg cttaccgctt tgcgggatgg tgagccggtc gcctacctgg
    10801 gcgtagtaat caacccccaa cccgttgatg gcgagaaact cgctggcatt aacgttcagt
    10861 cgtcgcgcga tgcagaacaa gaattcatct ttttgaatgg tataggtgga cggcaggccg
    10921 ggggtgggct caggttcctc ttcttctggc tggggcatgg gggttggcat ggctgcgggt
    10981 tgtgcttggg caagagcctg ggggttttcg ctttcgactg tgccttcaac cgccagcgcc
    11041 gtttgcgtgg ccgccagaat atcgatcata atttgggatt gagtggatac gggaaagggg
    11101 atttcgtcgt ttgctcttgt ggtatcccgt ggggcgcggg tggcagcgcg ttcgcacgct
    11161 gtcattaaca gggcgccaat gaccaggagt attacccagc tgaggattcg ttttttcatc
    11221 ttgcatctcc ttatccttcc cgcgtgtcag ggtagtgtgc atcattaaaa cgcgcggtgt
    11281 gataatatgt gtggggatgc cgcggcagtc aagacgcgcg ccacacggtt atcgtctgga
    11341 taaattacgt tgcaatcctt atgccaatta gacggagtgg tgatgaagat ttatttttct
    11401 tgttcaatta ccggtgggcg atctgaagaa tccgtttacc aaaaaattgt gagcgcgctg
    11461 gattccatgg gatatgaagt gccaactgcg cacctggcgt cgccggaagt ggtagagatg
    11521 gaaaaaacgg cagaccctaa tgacattttt gaacgtgata tggcgtggtt gagggagtgc
    11581 gacgcagtga tagcggaagt gtcttctcct tcacatggtg tggggtacga gatcgcttat
    11641 gcattaacgc tgggtaaacc ggtgttgtgt tgttaccagg cgggcaggag tgtctctaaa
    11701 atcattaccg ggaataccag ccctggctta tccgtgatga catatattgt ggaggaagac
    11761 gcacctgtgg tggtgaggga atttattcag cgattgggca accgacctgg cgtgcaagag
    11821 agcgagtatt agcgttctat tagagaccgg ttcgcaacta ttgactttta tcttcgacaa
    11881 agaatacaat taaaccatgg agtataagga ttactatcag gttctgggtg tggaccgcaa
    11941 ggccaccccg gaagacatca agaaatcttt tcgcaagctg gctatgaagt atcatcctga
    12001 tagaaataag gataacaagc aagcggaaga aaaattcaaa gaaatcaacg aagcatacga
    12061 agtgctgagt gatcccaaga aacgcgagcg gtatgatcag ctgggcagtt cataccaaca
    12121 atggcagcaa cgcggcggaa accctggcaa cttcaactgg aacgagtggt acaccaacca
    12181 gggcgggcgc gcacagcaag tgaacccgga ggactttggc gatatctttg gtggtttttc
    12241 cgattttttc agggtgattt tcggcggcat gccggtaagt gggcggcagc caacccgcca
    12301 acccggcact caacagtctg cgcaggcgcg ccgggcggct aactacgagc aaaaagttca
    12361 aatcaccctg gaagaagcct accgtggcac tcagcgtatt ctccagatca atgaacggcg
    12421 catcgaggta aaaattccag cgggggcgcg caccggcaca aaagtgcgtg tggcgggcgc
    12481 cggcccagcg gatgcctacg gtcgcggctc tgacatttat ctggtgatcg agctgctgcc
    12541 gcacgcgcgc tttaaaatca gcgggtctga cctgacgacg gaagtgaaaa ttgattacct
    12601 caccgctgtg ctgggcggta cggtaacagt gggcacactt tctggagatg tggtgctcac
    12661 catcccctcg ggcactcagc cgggccaaaa attccgcctg agcggcaggg gtatgccgat
    12721 actgaagtct tcacagacct atggcaacct ttacgttcag gtaaaagtgg aactgcccaa
    12781 gaaattaacc gatgaacagc ggaagctgtt tgaacaactg cggaagcatt cctagaaata
    12841 agaaggtgat cgaatgagaa aacctgttgt cttgtccatt gcgttgcttt tattggtggt
    12901 gctaatggcg tgtgtgttca ttcccagcca ggattggaac ccatcagacg tgttagagaa
    12961 cgtcgtcacc aaggtgccag atattgtttc accaaatgtg ccgcctgttt ttgttaacgc
    13021 ggaagacctc gccaatgttg accaggtgtt aaccaccctt tacgaaaggg taaaccctgg
    13081 cgtggtaacc atcatcgcca ccaccagaca aggggaggga agcggttcag gatttgtgta
    13141 tgattacgaa ggacatatcc tcaccaatta ccacgtggtg aaagacgctg agacgattga
    13201 agtggatttc gccagcggta aaaagatcta tggggatgtg atcgccacgg atgtcgattc
    13261 tgacatcgcg gttatccatg tggatatgcc cgctgctgaa ctggtgccac tttctcttgg
    13321 agattcagac cagttaaaag tagggcaaat ggttgtggct attggcaacc cataccggct
    13381 caccagcacc atgaccctcg gggtggtgag cgccaaaggg cgcattctgg attcattacg
    13441 cactacggaa gatttcactt catacacagc cggcgacctt attcaaacgg acgcagcgat
    13501 caaccccggt aattcgggcg gaccgttatt aaacctggcg ggccaggtgg tgggcatcaa
    13561 ccgcgccatc cgcacctcag gcaccaccct tgaaggaggg gcggtgaaca ccggtatcgg
    13621 ttacgcagtt tcgatcaata tcgtcaaacg cgtggtgccg attctgatca gtaagggttc
    13681 gtatgattat ccgttgatgg gtatttcaag ctcatccaac gaactaacgt tgagcatgtg
    13741 gaaaaagatc agtcatacgg tcacttcagg ggtttatgtg gtcagtgtca ccccgggagg
    13801 cccggctgac cgggcggggt tggtcggcgg cacccaggcg acggatgtgc agggatttta
    13861 cagcggggga gatattatca ccgctgtaga cggcttgccg gtgaatatat acggcgacct
    13921 tatcagctat atcttcaaga acaaatcacc aggagatgtg atcgttctaa ccatcatccg
    13981 caacgatcgt gaaatggagg ttccactcac cctgggttcg cgataacaac gaactgctgg
    14041 tcaaataaaa aaacttaaga ggaggtatca atgttcacag agtccagtct gcgaaagatc
    14101 caagattttt cagccgatta cccggtcgtc agcctgtatt tgaacacaga accaagccgt
    14161 ggtaacgcgg aaacacaccg gcttcgactg cgtaacatga ttaaagatat ttccctcaaa
    14221 caagatgctg aactcattga aaagttcttc agttacactt atgactggtc aggtcgctcg
    14281 gtagcggttt tttcgtgtgc gccggccgac ttttttcatg ccatcccgct ggctgtaccg
    14341 gtgaaagatt ttatccagat cggttcaaaa gcggcggtag cgccgctgga agacctgctg
    14401 caggattaca gcaacctggg ggtgatcctg gtggataaac aaggcgccag gctttttcat
    14461 tttcacatgg gagaattggt ggagcagcag gggtttttgg gcgaccaggt caagcaagtg
    14521 aaagcaggtg gagcttcttc ggcgcatggc ttgcgcggcg gggcgttgga tggcgggcgc
    14581 gcgatgcgcg agaccattga ccgcaaccaa cgtgaagtgg ctgaaacagc ggccaggttt
    14641 tttgagagca aacgggttcg gcggattatg ctcggtggaa cggatgagaa catctcgcgc
    14701 tttaagagcc acctgccaaa atcgatgcag agcctggtag tgggtagttt cgcgatgggc
    14761 atgaccgcca gcaacgccga agtgttgcag aaagtgctgg atcaggcgct ggaaaagtaa
    14821 aaaagtgaaa aataacagcc gcggtgttgg agatgaacac cgcggctgtt tttattgata
    14881 aggtcaggga aggttgtcaa ccagcaataa caggttggta tacagcatat ccagggcggc
    14941 gcgcgatttt tgaagcgtgg tttcttcgag gttcgcaact gcttcattca gccagttaga
    15001 gaaacccgag agggcttctg gcgtcaaccc ggccacttgc aaatcgctca ggtctttact
    15061 ggcaaagttc aacgcctggc gcgccgaatt cgtatcgccc ttctccagcg ccgcgcgggc
    15121 ggtgttgatg ttgcccatca acttgtaagc cagccgtttg atattggcgt tggcaacttc
    15181 aaccagttgt ttttcaatgg ttgcctgcgc agtggttaac tcatcctgcg tatccaccag
    15241 gttggcgcgc gtggtctcca gctcttgttg cacctgggaa ttctgcagtt caagctgcga
    15301 tacctggctg ttcagcgatg tgatttcttt ttcagcgctg gccgccgcgg ctgcagcggc
    15361 ttgcttggct ggctggtaaa ccgcaaagaa gagtgttgcc agcccgccaa cgtagaaaac
    15421 aagcgccaca atcacccagg gcaaaacttt ttgccaaaag ctctttttaa ccggctgaga
    15481 aggcgaaaga acaggtttgt ctttcacctc tggtacaggt tcagcggttg cctcctgtga
    15541 agatgaggtt ccttgggtgg cgggttcaaa acccggtaaa ggttctgtgg tttcagggct
    15601 atcctggggt tggggttcct tttggggttc catgggtttg tcctttcgta gcatggtgtg
    15661 tgaattacag gggaagtata tcattctaac cacgcttgtg cgccatgtgt gatgaaagaa
    15721 agacatatcc gaatggccaa gccggacata gggttataat gcgttatatg gatgctgcga
    15781 cattaccggg taataccgaa aatcgccgta tcgcccgcga cgcaggcacg gtgatgctgg
    15841 ctatcctgct ggggcaggtg ttcagcttgc tttcatccat tctcatcacc cgtgcttttg
    15901 gcacagggat ccaaaatgaa gcctttaacg cctccaaccg gctacctgac atcctttacc
    15961 agttaattgc cggcggagcg ctggcatccg ctttcatccc cacgttcacc acgctgctca
    16021 cgcgcggcga acgctcaaaa gcctggctgc tggcttctgc cattgccaat atcatcacgg
    16081 tcatctttat tgtgctgggg gtgctcatcg ctctctttac tccctgggtg gtgcagcgta
    16141 tcctggcgcc cggttttgcc gctgacccgg tcaagttcgc gctgaccgtt gacctggtgc
    16201 gcatccagat gatcgcgccg gtcatcttcg gtttaagcgg gctggggatg ggtattctca
    16261 acagtcaccg cagcttttta tggccggcgc tggcgccgat gatgtattcc atcggcaaga
    16321 tcctgggggt gctgctgctg gccccatcgt tgggcgtata tggcctagcg ctgggcgtgg
    16381 ttggcggagc gctgatgcat gggctgattc aaatgccggc gctgctgaag ctgccaggcc
    16441 tgaagtacac gccaactttt ggcatcaggc tgcccgatgt acgcgaggtg gcgcggctga
    16501 tgggaccccg tgtgttaggc gtagcatttg tgcagctgaa ctttctggtg aacacccgcc
    16561 tggcatcctt gcagccgctg ggcagcgtga cggcgctggc ggtgggcttt tcattgatga
    16621 tgatccctga ggcagccatc gcccaggcga tcgccatcgc cgcgctgccc gccttctcag
    16681 cgcaagcggc agccggaaaa ctggaagaaa tgcgcaattc gctggctgcg ctgctgcgag
    16741 ggctgctgtt gctggcactg cccgcttcta tcgggttgat cctgttgcgc gcgccgctgg
    16801 taacgattat tttccaacgc ggcgagtttg atgcgtcatc cacgcagctg gtggcgtggg
    16861 ctttactgtg gtacgccacc gggctggtgg ggcattcagt ggtagaaatc gtttcacgcg
    16921 cgttttatgc gctgcatgat accaaaacac ctgtgggggt gggcgtggca gcgatgagcc
    16981 ttaacgtggg gttaagcatt gggttaagcc ggctgttcaa cacctggggt tggatgccgc
    17041 acggcggatt ggcgctggca aactccatcg ccacctttct ggaaatgggc gcgttattga
    17101 tttttatgcg ccgccggctt acagggttag aaggaaggca tgtgcttaat ggtctggtaa
    17161 aagcgctcgc cgcggcaggt gtgatgacgc tggcgctgtg gggctggatg cgcgccaccg
    17221 ctggcgcttc cgcgtggttg gtcgccctgg gtggtatgct cgtgggtgca ctggtttatg
    17281 tgctgatgct tgcgttgttg cgcgtacctg agctgaagct ggcgtgggtt ggaacaaaac
    17341 gaagattgtt gaaaagaaca tcaggagaat cagatgaaaa acaatagcgg tcatgaattc
    17401 atggtgaaaa ctcgttacgc caacatcagc ccctcaccac agaatagcgg agtggtaccg
    17461 cagccgccgc tcgagctggc gctgcctgac ggcgcgcagt tgctgccgct gccgagacca
    17521 gctgacctgg tgctggctcc catggaattg agagcggcaa ttgaagcccg gcgcagcatc
    17581 cgggcttatg ctgaaacacc tctatcgctc gcagaactca cctacctgtt gtgggtatca
    17641 cagggggtca aacgtgtttc tgccagaccc tctactgcgc gcaacgtgcc ttctgctggc
    17701 gcgcgtcacg ctttcgagac ctacctgctg attaaccggg tggcgggatt aacccccggc
    17761 ttgtatcgct accaggcaat cgaacacgga ttactgagat tggaaacagc cgctgatatc
    17821 gcggggagca tcgcgcacgc ctgcattgac cagccgcagc tcactggcag cgccgcggct
    17881 ttcatttggg cggcggtggt ggagcgcatg ttctggcgtt acgtcgagcg tggttaccgc
    17941 tatctgcatc tcgatgccgg tcatgtttgt cagaacctgg cgttgggcgc ggagcagctg
    18001 ggctgcggta tttgccccat cgccgctttt gatgatgaac tgctcaacaa cacgctcggg
    18061 ctggacggcg aggagatgtt cgtggtttac ctggcgacag tggggaagaa aacggataaa
    18121 aaataaccgt tctcaggagc gcagcgaggt catcttgcca gcatactggc catctcaatg
    18181 taatcattgg cgatgacctt tttgtggttt atgacctctt ccattggaag aggtatgatc
    18241 tcgcggcccg ataaagcggt catcaccgcg ctcttgcctt ccaaaaggaa ctgaaccgct
    18301 ttaaccccaa agcgcgtggc cagcatacgg tcaaaggcgg tgggcttgcc gccgcgctgc
    18361 acatgcccaa gtatggtgac ccgagttgta aaaccgatat ccatggcgtc gatcatggcg
    18421 ccgagctccg tggtaggcgg cttgtaacct tcagccacaa ccacgatggc atgggtcttc
    18481 cctcgacggt aggcttcttc aatggcttgt gcgacctccc ggggaggtgt tttatgctca
    18541 gggatgagca ccagttcagc gccggtgatg attcctgcct gcaccgccag gtacccggag
    18601 gtgcgcccca ttgtctcaac cagaaaggcg cggttgtgag atgaggcggt atcgcgcagt
    18661 ttgtcgatgg catctacgat ggtgttgagc gcggtatcga cgccgataca catgtcggtg
    18721 ccgtaaatgt cgttatcgat gctcgccggc acgccgatga ccggcactcc cttctgcgcc
    18781 agttttagcg cgccggtcag cgagccgtct ccgccggcga cgacgagcgc gtctatctca
    18841 gcgttattga tgtgacggag ggcttctcgc tgactgccgg cttcacgaaa ctctgtgcag
    18901 cgcgcggttt gaagaatggt tccccctcgc tgaagaatac cgcccacttc gcggattctc
    18961 aactcgcgga acttgccttg aataagtcct tcgtaaccac gttcaacccc caggacggta
    19021 atgttgttgg cataggcggt gcgtacaacg gcgcggatga aagggttcat ccccggggca
    19081 tcacctccag aggtcattac ggcgatcttt ttgatacttt tcaccggttc gttcattgtg
    19141 ttcatcttct cttgtttaca ggttttcaac ctgaacgttc tctctgccta ccagctcagc
    19201 caactgctcg accagttctt tggtagcgtt ggtcgtgtgg ttcggaaaat ccagcaagtg
    19261 ccggtaaccg tgctcataca ccaaaaaaca gaaacggtct tgacccggga aagaattgag
    19321 caggccatgc gcccggtgga ttcggcgtgt gtctcgttcc ttttcaccac tggatttgat
    19381 ggttaccgtc actaacctgg ttccaccggg cgctgctgtg ttgggcggtg tttttattgg
    19441 cgacatgatc actggcggaa ggatgtaatc gcggggtggc gtatcaaatt tggtctcaac
    19501 gttttcgcgg ggttgctgcg ctgacggaga aacggcttcc tcctgcggca ggtcagtcgc
    19561 aggggagggc accacatctg tatcaggcgt gggaggcgcc gcgtggtgat ccgcaggggg
    19621 tgtaggaacc tgccaggagg attcttcttc tgagatcggt ggctcttcat aactctctgc
    19681 ctggtctgta tagcgtatgg tgtcaatatc ggtttgccag gtaccttcct gcccgtgagc
    19741 gaaaggacgg tctgaaggcg tttcgtcagt cgggatatcc tcctcgcgca aggctttgat
    19801 cacatccacc agtattttgg ggtcagattt atccaggtcc accttgcctt ccaccaggat
    19861 aacggattcc attttgacca acgcgcccat tctttcccag gctttgggga agatgaccag
    19921 ttcaacctca ccctgcaggt cttcaatggt ggcaaaggcc atctggttgc ccgtcttggt
    19981 ggttagcgaa cggatacggg tgaccatacc ggcaacactg accttttgtt tgtgctcggc
    20041 ttcaaccagt tccttggtgg cgtgagtggc tttctggcgg ataagcggca gataatgaga
    20101 gatggggtga tcggatatat acatacccga cagttctttt tcccattcga gttgcaggcg
    20161 gcggtccagt tgaacgccaa cgggcaaatg aatctcatct tccaaaccgg tcgcgttgcc
    20221 aaagatgctc atctgaccgc attccgcagc acggaagtga ctgttactga ctgaaacgag
    20281 ggtgtctgcc acttccagca gagcccgcct cgggccaaaa cgatccaacg cgccaacctt
    20341 gatcaggcat tccaggctgc gtttgcccac gctgtgcagg tcgacacggc gaataaaatc
    20401 gttaaggtcc ttgaagccgc cgtcctgcct ggcctggaag atcaaatcaa ccggaccaat
    20461 tcccacattt ttaatcgctc ccagcccgaa gcgtatggct gtcctgccat ccgggtcgtc
    20521 ttctatacaa aaatcccatt cgctgttgtt aacatcaggc ggcaagatct cgatgcccat
    20581 cgagcgggca tctgccacgt aaaacgccac cttagtgctc tcatctttat tggcagagag
    20641 cagcgcggtc atgtattcca ccgggaaatg cttcttcagg taagccgttt cgaccgcgat
    20701 aacgccgtaa tcggcagcgt ggcttttatt gaaaccatag cgggcgaaat tatcccaccc
    20761 ggagaagatt tcttctgctt gcacagccga aatctgattc ttctgagcgc cggcgataaa
    20821 ttttacgcgg tgctcatcaa tagcgttttt gttcttcttg gagatggctt tacgcaggtt
    20881 atctgcttct gaagccgtat agccagccag ttctatcacg gccctcatta actgttcctg
    20941 gtataccggt aaaccgaagg tatctttgaa tattggctcc atcaatggat gctcgtaggt
    21001 gatctcctct tcaccgtgca tgcgattgat gtagttggga attttttcca tgggcccggg
    21061 ccgaaaaagc gccaccatgt cgatgatatt cgccaggttg tgaggtttca tctgcatgat
    21121 gtatcgggtc atgccgctgc cttccagctg gaacaggccg gcggtgcgcc cctggctgat
    21181 aaactcgaat gtctccgggt catccagggg aatattgtgc aggttgagcc cgatcttgtg
    21241 gcgctttttg atcaggtcac aggctttgtg gatgatcgtg agggtggaaa ggcctaaaaa
    21301 atccaccttg agcagaccca gcttggtgat gatgttcatc tcgaactggg tgaccgactt
    21361 gatgggagat tcctcggcgt tactggttgg gcggtgcagc ggcaggtact ccagcagcgg
    21421 cttgtcggtg atgaccactc cggcagcgtg tgtgccggcg ttgcgggcca ccccttccat
    21481 gcggcaggcg gtatcaatga gttctttcac atatccgccc tcgctgtaaa ccgtctggaa
    21541 ctcagggcat ttttccagcg acctggggat ggtcaccgct tcctcgggga acatcgccgg
    21601 aacggtcttt gaaatgcggt cgacctctga aagcggtaca tccatcacac gaccgacatc
    21661 acggatggcg gcgcgcgccc caagggtacc aaaggtgatg atttgcgcca ccttatcgct
    21721 gccgtatttt tcagcgcagt attgtaaaat cagggaacgt ttgtcgtcct cgaagtcaag
    21781 gtcgatgtct ggcatttcga tgcgagacgg gttgagaaag cgctcaaaaa acaaaccgtg
    21841 cttgaggggg tcgatgaggg tgatgttgag cgtgtaggcc acgatcgaac ccgcgccaga
    21901 cccgcgggtg ttgtaccaca cgttattttc agcagcgtag cggcacagat cccacacgat
    21961 caaaaaatac gcatcaaagc ccatttcgtg gatgatcttc agttcgtgtt caaggcgttg
    22021 ccttacttcg gggtcatcga cgcgcccagg atatttcacc tgtaaaccgt cttcacagag
    22081 ttttttgaga tacgtttgcg gggtgaatcc atcgggtacg gtaaacaaag gcagatggta
    22141 gcccttctgg atgaggttta cattgcaacg ttcagccact tcaagggtat tggtcaaggc
    22201 tgaagggatg tctggaaaga gcgctgccat ttcttgcggt gagcgtagat agaaggtgtt
    22261 cgcgcccatc ttcatgcggt ttgggtctga gagcagtgac cccgtttgaa tggcgagctt
    22321 aatatcttgc aggtgcgcgt cttcctgttc aatgtaatgc aaatcgttgg tggccacgca
    22381 cctgaggtta tagcgtttac ccaggtcaat taatgtgcgg ttcaactccg gcagttcgcg
    22441 caggggatgg ttttgcaact cgatgaagaa atggtcttta ccgaaggtgt tgatatacca
    22501 ttcgagcact ctctgcccgg cttccactcc tttgttgagg atcgtacgcg gaacctcgcc
    22561 agacatacac gaggttgtgg cgattaaacc ttctttgtgg ttttcaagga actcgtggtc
    22621 aatacgcgga tagtaataaa acccttccag ctgcccggcg gtagcgattt gcaacaggtt
    22681 tttataacca atttcgtttt cagccaacaa gaccaggtga tacgagtgct tgtctttctg
    22741 aggatcgcgg tcgctcatac ggcgggcggc cacatacgct tcgacgccaa tgatgggttt
    22801 taccccagcc tcgaccgcgg tggaaaaaaa ctcaatgacc ccgaacatcg tgccgtggtc
    22861 agtgatggca atcgaatcca tgcctaaatc tttaactttt tggatcagct tcttgatgtt
    22921 gctgaagcca tcgaggagag aatattcagt atgaacatga aggtgggcaa aagacatgac
    22981 agaccgcggt ctcgctctct actgataatt attaaaaata ttatagcatg tggcgtttct
    23041 ccgtttctta aggatcattt atccccgtgt aattttaaga aagcgcgagc ccccgcgcgg
    23101 cgaaaaggct caagtagcgg tgcgagaccg taacgaagca aacaagccaa aaaattgaaa
    23161 acggaccagg ctgatttgtt atgagagaaa agggcaatcg gctgggcgta cgcctaggtc
    23221 gctgtcggcg aacgtgatac cagcgaagtc agcgaaagtc agattgcgca tggtgagccc
    23281 cttgctttgt ttaagcctct cgaagagttc aaggtaactg gcttcaatac gggcgtccca
    23341 gtccaaagcg agcgcgccgg ctgtggcgcg gatgagggct gtgtcggcac cttcaatctc
    23401 aatgaaatga ccatagggca gttcatccag agtgatgaaa aggtcgttaa ggaggtaaac
    23461 cgttcgccac ttttcgtagc gcacggtcat ttcgtatccc agaccttcga ggatcaggcg
    23521 cgccttgtcc gggttatcta tgaacacttc gtgttcgggt ctgacgcgca cgccgtctat
    23581 aatagaggaa ggacctttga aggtgagaat atcccgcagg tcatggcgca ggcgcaacgc
    23641 ccgaccagaa agggcaagcg cgccatccgg agtgtcgaag cgctcgtttt tctcgaaggt
    23701 tcgcgcctgc ttctgttcag ccccaagcga gagcaacctg tctcgcaaag cgggcaggtc
    23761 agacaggaaa aatttcactt ccatttccac gccgggtttt tccagggtca acctccaggt
    23821 acatcatgtg gtggcttgcg ggtttccttc atgtagggtt ccagcaacca tgctgatact
    23881 aacacaaata acaacagcag cacagatacc aggagcggaa ggagctggtt tttaaacggc
    23941 agcgggttac gagtgagcac gagacggtca aaccagtacc acaaaaaaaa cagcagactg
    24001 tttgcctggc ccagtcttgg cgcgaacgcc ctgcgccccc agatggcaag cgctgcggtg
    24061 aaggataaaa gagcgagcaa cactgtctta aaaaggaggt agaccgggtg aggaaagacc
    24121 cccgctgcca gcagccagtt ccaggagcgc agcgcctgca aaaagcctgc gagattgatc
    24181 agaaacacaa gaagaaaagc gctggcgaac agatgcagca aaaaaggagg gtggggcgcg
    24241 gtcgtctttt ccatcacagg tgttgtggat tatagcacgc gcccttctgg gcagcgccaa
    24301 cttgacagcc gtttaaagtc ggatatactg acaaagactt cgcgagaaaa agaaaaaggc
    24361 gcggatgaaa atctcaatct gggatatcct gacggtcgtg ttgctgctta ctgctctcgt
    24421 gctgctggtg gtggtgatgg tgattttcat ccatcctgat tctcgcatca acccgttccc
    24481 ttttccctcg ctcccggcga cgattgtaat ccccacgctt acccccaccc tgttgcaaat
    24541 gccgcccacc tggacgccca ccccctttta ggagagaatc caggttctca ttccttcacc
    24601 ctgaacgaga tttcttctac ccgacacaaa gagagagttg acctatgagc gaacagataa
    24661 caaaagaggt ttttgaccat ctggttggat tggccgcgct gcaacttgag atggaacagg
    24721 ctgaatacct gcgtaagcag ttaaacaacc agttaaaagc cattgctgaa ctggaagcca
    24781 tcccgctgga aaaggatgtg cccatcagtt tgcacggggt gccttatgaa gcatcgctca
    24841 gcgccccgcc gcgcgaggat gagtggaaac cttatcctga cccggcaggt attctggcac
    24901 aggcgccgca ggtggtggat ggctacattg tggttcctga tatccctcat accgagttaa
    24961 agtaggtcgt atggaaattt gtgatctcaa cgcacttgaa ttggcgcgcc tggtgcgcag
    25021 ccggcaaacc tccgcggtgg aggtggtgaa atcctgcctt gaacgcgttg cccaggtaga
    25081 cggaagaccg ggttcgctgg agcaaatgcc gctatccgat gaagataaaa aacgcgtgca
    25141 cgccttcatc acactgactg gtgaaaacgc gctgcagcag gcggagaaag tggaccaaat
    25201 gcttgccgct ggcgaagatc cgggcgtgct cgctggtgtg ccgttcacag taaaagacat
    25261 tttttgcgta caggataccc tcacaacagc ggcttcaaaa atcctggcga actttacggc
    25321 tccctacaca gccaccccgg tgcagcgcct gcaggatgct ggcgccatac tgctgggaaa
    25381 ggtaaacctg gatgaattta cgtatggttc ttccaatgaa tcctccgcct atcaacccag
    25441 cccgcgcaac ccctggaata ccgaccgcgt gcctgggggc tcctcagggg gcagcgccgc
    25501 cagcgtggcc gctggtgaag cgccgctctc tttgggcact gacactgccg gatctatccg
    25561 tcaacccgcg gcgttttgcg gagtggtggg ggtcaaacca acctatgggc gggtttcgcg
    25621 ctatgggttg atcgcttttg cctcctcgct cgattgcccc gggccagtgg cgcgcgatgt
    25681 gaaagatgct gccgccatgc tgggggtcat tgccggcagt gacgagcgcg atgccaccgc
    25741 cgcgcggctg gcagtgcctg attatctgga aggcattgaa gagggagtga aggggctgcg
    25801 cattggcttg tcgccggatt attttcgtat tacctacccc gacccggcga gcggagagct
    25861 gctcagccag ccgctgcctg aagaagttgc ccgcgcagtg aatgaagcgg ctgaaaaact
    25921 gtcaacgatg ggagcggata ttgtggaggg cgtacccatg cccaacacac gttttggcat
    25981 cccggcgtac ttcgttatca gccgtgttga agccgcttcc aacctgcacc ggtatgacgg
    26041 ggtgaagtac gggctgcgca gtgaagcgcc caccgcagac ttgaaagaga tgtataaaat
    26101 aacgcgcaac caggggtttg gtccgcaacc caaactgcgc atcctcatgg gtatgtatgt
    26161 gagcgccgcg caatatagtg aacaatatta ccgtcgcgcg ctgcaagtgc gcacgcttat
    26221 ccgccaggac tttgaggcga tatttgaccc ggctggcgcg tacagactgg acgcgctgct
    26281 gacacctacc acgcccacca ctgccttcaa gatggcagcg ttgtatggag actccgtgct
    26341 gatgcagtac gctgaccagt tgacggtgcc cgccaaccac gccggcgtgc cgggtatctc
    26401 gctgcctgcc gggctggata acgaggggtt gcccattggc atccagctgc ttggcgccga
    26461 ttatgcagag aaaacgctgt tccgcgcagc gcgggcatac gaaatgacca cgcaagaggc
    26521 agattggcgt aaacaaaaac ctgaagtgct gcgagggcga tcatgacaga ttacgaagcc
    26581 gtcatcgggc ttgaaaccca cattcaattg aacacggtca ccaaggtgtt ctgctcatgt
    26641 aaagccgact catggtttga cccgcctaac accaatattt gcccggtgtg ctgcgggctg
    26701 cccggggtgc tgccagtgct taacaaagcg gtggtggaga aaggcgtgtt gctggcgcgg
    26761 gcgatgaacg ccgagatccg tccgctctct ttcttcgacc gtaaaaatta tttctaccct
    26821 gatctgccca agggctacca gatctcgcaa ttcgatcagt cgttggccag gggaggatac
    26881 ctggacctgc cgataccagc cgcggcctcg cctgacaagc aagcgtacac caggcacgtg
    26941 accatatgga aattgcatat agaagaagac gccggcaaga ccaaggtaag caaccagcgg
    27001 cgtctgattg actttaatcg ctgcggcgtc ccgctggttg aaatggtcac cgggcctgac
    27061 ctgcgcactg ccgacgaagc cgcgcagtat ctcattcgcc tgcgccagtt gttacgctgg
    27121 ctgggtatct ccgaagccga tatggaaaaa gcgcacctga gatgcgacgc caatgtatcc
    27181 atccgtgtgc ggggtgaaac caagctgaac ccgaaaacgg agatcaagaa cgtcaattcc
    27241 attgacgcag tcagagaggc gattgcgcgg gaaattgagc gccagattcg tgaggtagag
    27301 gcaggcaggc atatagaagc ctggacgctg gaatgggacg aagatagcgg aacactgaaa
    27361 aagatgcgtt ccaaagaaac agaagccgat taccgctact ttcgtgagcc tgacctgctg
    27421 cccatccgcc tggatgaggg atggcaaaga cagattttgg cggactttcc tgagctgccg
    27481 ctggaacgac gcgcgcgctt tgtggagcaa tataatctac ctgaatatga tgctgacatc
    27541 ctcaccagcg agcgcaaact ctcagattat tatgaaaaca cggtgaaatc atacggcggc
    27601 gatcccaaaa aggtctctaa ctggttgatg aacgatgtgc tgcgcatgat caacgatcaa
    27661 gggttgaacc cgcaagagat gcaactgacc cctgacgcgc tggcggagat attgaaacta
    27721 gtcgacgcgg gtaccattaa tatcaacacc gggaaggcat tactgcaaaa agtgcaggag
    27781 agcggtaaaa aacccggtgc tattgtggcg gaggaggggt tgggactggt gagcgatgac
    27841 agcaccatcc gcgaggtgtg ccaggcggta ctggcagaga gtcccaatga agtcgcagcg
    27901 taccgcgcgg ggaaggtgac gctgattggc tggtttgtgg gcggagtgat gaaaaagatg
    27961 cgcggcaagg cagacgcctc cctggcgcgc caaattttgg aagacctgtt aaacgagaaa
    28021 aatcgctaag agagcgaaca caaacgccca tcccccaaaa gccattcttg ggcgagggtt
    28081 tgttcgcagc ggtttatggc cagccagccg atacgcgctt aaaggtctct tgcaactctt
    28141 ctggtaacac cctggtttca cccgtggtcg acataaaact ggtgtcgccg ttccagcgcg
    28201 ggacgatgtg tatatgcgcg tgttcggcga tgcctgcgcc tgccgccacg cccatgttaa
    28261 tgccgatatt aaagccatct ggctggtaga tcttgcgcag caccaggatg gcctgattga
    28321 ccatttcaat catttcaaaa cgcacatcgg gggtgagcag ttcaatactg gaaacgtgcg
    28381 cgaagggcac caccatcagg tgcccgctgg tgtaggggta tcgattgaga atggcgtaga
    28441 ccttttgacc gcggtgcaca atgaggttgg caacgctgtc tgcttgtccg ggcgaggtgc
    28501 aaaacacgca cgcgccggag ttacttgcgt cggtaacgta cttcatgcgc cagggagacc
    28561 agagaatgtc catttttccg ccttctttct tttactggct taattctaca cgcgatatga
    28621 gcatttttta gacccgctta aaaacacgcc tgttctgttg acaggtgctc aaggtcgatg
    28681 ttatcctatc cctatgttgc aaccacccct gttcccggtg aagacgctca gcgtcagcga
    28741 gatcacgcgt tacctgcgtg cgctctttga gtctgacgag atcctgagcg atgtgtgggt
    28801 acagggagaa gtgtccaacc tttcgcgacc ctccttaggg catatttact ttacgctaaa
    28861 agatgctttt tcttcaataa aatgtgttat ctggaaacaa accgcatggc ggctgccttt
    28921 gacgcttcaa aacggtatgg cggtggaagc gcatggcggg gtagacatct atgagcgcga
    28981 tgggcagtac cagctctatg tggatgcgct gcgcctgacc ggggaaggcg cgctttacca
    29041 ggaatttctg cgtcttaaaa cccgcctaga agccgaagga ttattcgctc ttgaaagaaa
    29101 gcgcccgtta cctgaacaac cgtctgctat cgggctggtc acctcttcca gcggagcggc
    29161 gctgcaggat atattgaata cattgaagcg gcgctttccg ctggttcagg ttattctggc
    29221 gccctgcgcg gtgcagggag aagacgcccc tccgcagatt gtgaaatcgc tggcagccct
    29281 gaacgcgcaa aaccaggccg atgtcatcat tgtggccaga ggcgggggtt ccctcgagga
    29341 tttgtgggca tttaatgacg aaggagtcgt gcgcgccatc acggattcgc aaataccagt
    29401 gatcactggt attgggcatg agacagattt tacccttgct gatttcgctg cagatgtccg
    29461 cgctgccaca cccaccgccg cggcagaaca ggtggtgccc gacgcggctg agatcaaggt
    29521 gaaattgagc gatatggcca atctgctgtc cgccaccatc cgacagatct gcggcgcaca
    29581 gcgtggtcgc gttcaggata gcgccaggcg gctggagctg caatctccgc tgtggcgggt
    29641 gcgcaatgac cgtcagcata ttgatgaatt acaggcgagg atcgctaata accaggcgca
    29701 taggcttgag ctgatgcgca gcaacctggt tcatttacaa aagcggctgg ttaatctcag
    29761 cccgcaggcg gtattgcagc ggggctacgc catcattaaa acagcagagg gtgtattggt
    29821 gaagagcgta cgccaggttc accgcgggga tgtgctcgat atccgtgttg ccgatggcgc
    29881 gattctttca aaagtgctaa ccgagcccac aacagaacgt aacggaggga ttgccgatgg
    29941 ttaaatcagc atcaacgctt tcagacttga gttatgaaca ggcgttcagt gaacttgaaa
    30001 ccattgtcgc cgcgctggaa agcgaacaaa aaccgctgga cgaagcattg caactttacg
    30061 aacgcggtca actgctggtt caatattgcg ccgccctgct cgaccaggct gacctgcgca
    30121 ttcaacagct taaccctaac aaagaaacca cagaggacgc tgcgtgagca ttctggcggg
    30181 ttaccccgag ctgattgcct gtatcatggg gtggctggtc tcttcccttt taaaaatacc
    30241 gacctattac tctatacacc ggcgcatcaa cctgaagcaa gcttttggca ctggcggcat
    30301 gcccagctct cacgcggcga cggtgacggc gaccacactg gctattggcc tgttcagcgg
    30361 gttcgaccac ccggccttcg cgttagcggt ggcggtctcg atgatcgtta tatacgatgc
    30421 cgcaggggta aggcgcgaag ccggttacca cgcggtcatt atcaaccgcc taatcgatga
    30481 atacgtgaag ggccccctga tagaccagaa aaagttgaag gagatgatcg gtcatacccc
    30541 gttggaagta gtgggaggtg tcatttcagg gaccctgacg acgttaattc tctggctcat
    30601 ctggcccaag tgatgcctgc cgatggcagg cacttctctg aaaacgcatc cgtcttttta
    30661 atatgctctg gtctacgatc gcttaaaagc caagttctga aaggcttttt tctacatcgg
    30721 gtttcgcgtt tttccccttc agttcctccc agaaccggtt gtcatagcgg cgagagcgca
    30781 cgaggatggg catgggcacc ccccacccca gcaggagcac ttcctctttg ggctgcaggc
    30841 gtgccagcat accgcgcaac gagtctcgcc cggccaaacc agatagcaca gcggcgatat
    30901 cactctcgtc tcccagccag ccagaaacac gcgtgcctaa ctgcgacatc acctcatcgt
    30961 agatttgcga agggcgctgg tcgatgatga gcaaggtgac gtagtatttg cgcatttcac
    31021 gcgcgatggt gctgaaagtg gtctgtgagg ccatttcgcg gtttaagatt ttatgcgctt
    31081 cctccacagc aattaccagc gggcgcggtt cgttttgccc gctgctgcga aaatcgttgg
    31141 tcatcttttc ccaggattca cggatcttgc gtgtaagcag gttggaaacc agcaggtaat
    31201 ccaggtcttt ttcaaagtcc ccaaaggaga gcacgacgtg cttgccagcc ttgagtgtat
    31261 cgatgatgtt gtttaacgaa ttgaccgcgg tcttgtctgc ggggtcgcgt tcgatgatat
    31321 aaggacggtt aaaaacgttg cgcagcttgc tatgtaagcc ttctgccgcc gccacgttca
    31381 ccccttcctg gttggcccaa aaagccacgc tgccgggggc ggggactttt ttcccttcat
    31441 catctatgat cgtcgcccca ttagccatat tcttaaactc ggtataccag cggttgggcc
    31501 caaatgagtg caccagcgcc tcaagggtgg tgggggtggt ctctctgaga tttaattcac
    31561 gggagagcag ctcgatatct tcaggctgga tatcagattc atagatctca aggtgcaggt
    31621 ctggggtgcg cttgcggatg gtggtttcac gccccagccc gaccaccctg acgcgctcag
    31681 cgtatttgcc tttgagcccc ggaaccgcct gcccggtgtc agaggaagta tcatcgaaac
    31741 cgtactcgtt atgcatatcc agcaccagca cagaggcggc gttgtgttga atgagaccgg
    31801 ccaggatgat gcgcgtgagg aaagattttc cggttccagt ggcgccaaag atgcccgaac
    31861 tgcgctgcac aaacttatcc aggttgatgc ataccgggtg gtcctgttca cgcgtgcgcc
    31921 caataataaa gaagcccttc gattccgggt caccaaagat ttcagcaaca tcaccggcgg
    31981 atgccagcct ggcaacggcg tgatgcgagg gaacagtctt gataggcagc ggacgggggg
    32041 cattttctcc ctggtgttcg cgcgcccagt cctcgtattc agcagacccc ggctcaggcc
    32101 cgcgctcgag cattaaggcg ggcatcacct cgaggttggt gtaaagcgtt tggctggcca
    32161 gcagacgggc ggcgggttgc gaaaggcgcg cggcattctg cagttcagcg tagcgcgggt
    32221 caagcgcgcc cagctgcagg tcagtgacca ggccgtagaa ctgccagtta ccgctttcga
    32281 gtaccacaaa cccgccctcc tgcacctcct gcgcgggcag ggtaagccga acaaagaggt
    32341 tggctttgag tccgccacca accacgtaac caatagggcg gtcgccaggg tctgatgggg
    32401 aaagcgtgcg gttaagatca gcggttgtca tttttttacc tctttctatt ccatcaccac
    32461 tgagactgcg ctcaaaacgt ccatcaactg aggataatca tctttgagca gagcgataac
    32521 ttcgttcagg cagcggtcac gccaaccctc ggggtctggg tgatgcgcca ctttgaacac
    32581 ctgcacgagg tgagcggtca ccaggtcagc ggcaggtatt tcctggttac gcagtaggcc
    32641 gcgcaggtaa cccagcctgg cattgctggt gaagcgcagg atatctgcat cactgcataa
    32701 gaatatttca tccagtgtca gcggagggcg gggcacgcgg gcatgcgaaa cccgaccggc
    32761 gctatcctcc cagccaatga ataccaggct gatctcgatg ggcacgttgc ggttctggcg
    32821 gttatcctcc agcacttcct cggaaacctg cgcgctgacc agctggcgca ccagaccgtc
    32881 atcatcgatg tgaatgtcgt acaccaagcc gtaaatgcgt gtgccatcgc tcaaagggat
    32941 gagcaccatt gctccaaaag ggggagtacc agcctggctg acacggcagc ccatcacaca
    33001 actggtcgtg ttggctcgca gtaatcgtcc aataggtatc gcgttcatgg gttgatcctc
    33061 gttcttcctg gatggtcttt atgcacctgt ttttcagacc tgctgccggg caggatgccc
    33121 tgacgggcaa gttcgctttc gatcatcgtg gtcaattgct gccggtcgct taactttacg
    33181 acagcaatct cgtgggcgcg gatgagcgga taggggtaag gcgcctcgcc gctcagggcg
    33241 gattgttcca ccagtgtggt gtgcagggtc tgcaccgatt gcggattttc cgctacccac
    33301 tgggggattt ccacgcgagc gaaagcagag cgagaggggc tgcccacgtt gaggtagaaa
    33361 aaatgcagcg ccttacggtc tttatactca tccgcagagc gcgagtgcag gcggaaaatt
    33421 gcagagcgct cgccaggctg caggatgctg tgaaaaaggg caatgtcgct gatgccgctg
    33481 acagtcggtt ggctgctctc ttcgttgagc gtaagcagcc tgaccaccag gtcggcacgc
    33541 ggccggctga cgtagccggc ggtgatcaca tccgcaattg ccaggtcgtc tagcgcgcgc
    33601 aggtagtctt cgaacttctc tttaaaggca gcgtcagtcc gcggttcatg atacagttcg
    33661 agcggtccgt cagtaagggt gatcacaggc ggggtggcga ggtgcgctaa ttgcgccagg
    33721 gtgcggcgtt cctgcacgtc gcgcagtaat gccaccaggt cttctgaagc cattcctccg
    33781 gaggcgtaca gttcatctcc aaataaaaga tgcgattcag tccgctccag cggtacgcgc
    33841 ccgctgtgcg gttggataca aaacacaccg atgttgacca gcccataaag cacagaatca
    33901 tgcgggtcag ggttgatttg cgaaccgtct gccgcaaaaa tggtgctggc gggcgcgggc
    33961 agggtgcaag gaaagtgcgc gttgagagat tcatgcagcg gaacagcgca gcgcagccct
    34021 ttggtttttg ccgcggcttc ctccacctgg cgctgtaaac ccttcaggtt ttcagcattt
    34081 tcatttaatc ggacggataa ctgttcaagg ctttgacgta tttctcccgc gcgggataaa
    34141 gcatctctgc ccatatcgcg tatctgctga ctgaggagaa ggtaattaac cggcacgtaa
    34201 cccctttaaa taatcgaaca tttgtttgaa ttatacataa ttcaatcgcc aacggcaagc
    34261 ggttatgggg attcgaacct taaaaattag caaacactgg agcaatgaca gtgattacca
    34321 tggcatacag ccatgcctga ttctcgcaac tggatgtata atgcgccttc tggaaaccac
    34381 gcgcatgaat agttgggaaa ttctcttgat tgcgttaggc ctggcgatgg attgttttgc
    34441 ggtatcgctc ggcatcggca caaatgggat gaaaaaaacc gccagaatgg tattgcggct
    34501 ggcgttccat tttggcttat tccagggtgg tatggcgttc atcggctggc tggctgggcg
    34561 tcagatcgcg ggcttgattt ccagttttga ccattggctg gctttctttt tgctgttatg
    34621 ggtgggcggc aggatggtca ttggcagtac taaacaggaa gaggagtgcc ggaaaaccga
    34681 ccccaccagg ggcggctcgc tggtagccct ctccgttgcc accagtatag acgcgctggc
    34741 agtgggcttg agcctggcga ttgtggatgg ctctattttc atcagcagtt ctatcatcgg
    34801 gctgacttca tttgcccttt ctgtgtctgg ctacctgttg ggcgacaaac tgggttgccg
    34861 atttggtaaa cggatggaat tgttgggtgg cttgctactg atcggaattg gcatcagggt
    34921 gctgttgagc catctgttgg ggacctaaat aattcttgac ccgactcagt gatgggacta
    34981 taatgtttca cattattgcg acaagcagga aacagaggtg atactcatgc cggtatacac
    35041 atatcggtgc gaaaactgcg gtattcagtt tgatcagact caaaaatttt ctgacccggc
    35101 gctaacccgc tgccccgagt gtggaaagaa gagcctgaaa aaggtctata ccccggtggg
    35161 gattgtgttc aaagggtcag gcttttacgc caccgaccac cgttcaccgt caggcgctgc
    35221 acataattcc aacacgaaac acgaagaagc caagagcact gaaaagacgg aaacgaaaac
    35281 tgagaccaaa gcagccagtc aaacaacggt cgaatccacc agcaccccga gcggcgataa
    35341 agcctgaaac cctccatttg aagatcaacc taccaggaag aatcataccg ggttacgggt
    35401 tgttacccat tgaaagggat gtgataggat ttgttaaacc gttgatagaa aatgatagtt
    35461 tgcggaaaat aaactcccga gcatatagcg aggtatatca tggcaaaacc caataaacaa
    35521 acaggcgcgc aaaagcggtc aaaaataaca tccagccagc gcaagatgcg cgcgcaacag
    35581 ataatcctcg cggtgatcgg tatcgtggtg atcatttcca tgatactggc actaaccatg
    35641 aaattctaga agaaatatgc ctaaaaaaca aaagtggttt tggttggcag cgctgattgc
    35701 tgcctggttg tttgattttt tgttctggag caaaggctca ggtgtatcct tcttcatctg
    35761 gatcgccatc ctgctgctgg cgggttacac gctggcgtgg agcgagggga agaagccgag
    35821 cgcattgagt gtgctgctta catgtttcat tctcggtttt gccgcggtca tagccttgcg
    35881 caacgaaccg ctcacacggg cggtgagcat atttctgacg ttgaacggga tgctgctgct
    35941 gacggcaacg ttcttgaacg ggtactggcc gtggtacagg ataaaggatt atgtagtgga
    36001 actggtccgg gtgatcggag cgggattttc tggcgcgatc tggctgttgg caaaaggaaa
    36061 aaccccgccg cccgtggatg gctctccagc gaaaagcgga tggagcaaag cctggccggt
    36121 cattcgcggc ctgctcattg cgctccctgt tgtggctgtt ttggggttgc tgctggcttc
    36181 ggctgaccag gtcttctctg actgggtcaa gcgtattttg aatattgaaa aactgccgga
    36241 atacctgttc agactgtttt acatcctgat cattgctggt tttctggtgg gcgtgtacct
    36301 gaaggcgata taccccagga agacggccca aaagcccgaa ccaaaccagg cgtggcttca
    36361 acctttcctc ggttggactg aaagcgggat tgtgctgggg gcggtgaacc tgttgttcat
    36421 cattttcgtc atcatccagg tgcgctatct cttcggtggt gaagccaata tcagtgaaac
    36481 tggctttact tacgctgagt acgccaggcg cggcttcagc gagctggtag cggtggcggt
    36541 gttgagcctg ggtttgtacc tgctgctggg tacggtcaca aaaaccgaga gccaggggtc
    36601 caaggtcggg ttcacagcgc tggcagtgtt gttgatggtg aacgtgctgg tgatcctggc
    36661 ttcctctttc ctgcggttga tgttgtatga gaacgcttat ggcttttcac gccctcgaac
    36721 ttacacccac attttcattt actggctggc ggggttgatc ctggcggtga tggtgctgga
    36781 gctgatgaag cgtcgaggac gcatcgcgct ggcgctgttg gtggcggtgg tggggtttgg
    36841 cgcgacgatg gccatcctga acatcgatgg ttttatcgtt cagcgcaacg tggcccgagc
    36901 cgaagctggt gctgaactgg atgttgtcca tctgaccacc ttatccgctg atgcggtgcc
    36961 gcagctcctc caaaagtatc agtcgcctga gttaagaaaa gaagtgagag aaggcttggg
    37021 cgcaacgctg gcgtgtatgg aaaaggccat ccaagacccg gcagatctcc cctggcagga
    37081 atacaacttt ggccaggcgc gggctgcgca gttgttacgg gaaaacgcgg acagctggag
    37141 ccaatataag gcttaccaga cggaccggga ctgggtggtt cgcctgaatg ggcagaggat
    37201 ttactgcgcc tatttcatca tggattaact ggcataactg aataagcagc ccactctgag
    37261 cgctcaggcg ggctgctttt ttctatggga atgttaaatg aggtcaagtt cgtttttcac
    37321 cggggagaag agcacgccat cccagccgaa aacagacaga tcgatggaac cctggtcatc
    37381 aaaaaggata ccctcttctt ccaacaggac acgctgcctt tggctgccca taccaaagcc
    37441 gcgtgggctg attttcccct gcgcgttgat aatacgctgc cagggcactt cttcagcaat
    37501 atcacgccca cggtagacgg gcagcgctgc cagcgcaaac cccaccatct gcgcggagca
    37561 gcggccggcg atgcgggcga tctgcccgta agtggccacc cgtccgcgcg gcacctggcg
    37621 cacaatggcg tagatgagtt catataagcg aggctcttct tccatgtcac tgttatttta
    37681 ccgcttcagg cttcaagcag cgcgtatagc cttcatggta gaattcgaag catgaaaaga
    37741 tggcagttct gggtgggtct gttaatcagt gttgcatttc tatattttgc cgttcgcggg
    37801 ctcaacctgg ccgatttttg gaaagcggtg aaagatgcca attactggtg gatacttccg
    37861 ggagtggcgg tcttttttgc tgggctatgg gtgcgggcgt ggcgctggca ttacctgctg
    37921 gggccggtga agaagatacc cacccgcatc atgttcccca tcacctgtat cggctacatg
    37981 ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    38041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnacggccat ttttttggcg
    38101 gcgttgctgg tgttcttgct ggcagcgatg ttccccagaa caaccgccag gctgggtggc
    38161 tggttcattg agcgcctctt gccaccgcgc gcgcgtgaaa aaaccaccgg catcatgctc
    38221 aagttcctgg atggacttga atccttaagg tcgccattca acattttgat ggtctttttt
    38281 acttcggtca tcatctggtt gctggagacg gtgaaatact ggtttgtgat gcaggcgttt
    38341 actttttcag tgagcttttt tgccttgatg ctgctgaatg ggatcgcgaa cctggccact
    38401 accattcctt cggcgccggg gtatatcggc acctgggaag cggtgacccg ggcggtgctg
    38461 gtggcatttg gcgtgcccgg ggctgaagcc ctgggttatg gcgtggtact gcacgtggcg
    38521 ctgtggctgc cagttacgct ggtcggcgcg tacctgatga ccagagaggg tatcaggtgg
    38581 agcgattcgc tgcgacagga ggcccgttca ggcgaagctg aagcaggcgg ggaaatttgc
    38641 gataaggaga gcaataaatg aaaatcgcgg tgattggcgc cggttttggc gggttggcag
    38701 ctgcctatga cctggtaaaa gcgggaaaca cagttacgct ttacgaagcg gagagcgccc
    38761 ccggcgggct ggccgcagga ttcaaagaac caaattggga gtggtcagta gagaaatact
    38821 accaccactg gtttacctca gatgaacata tgttccgcct gttcagagaa ttgggcgttg
    38881 aagacagggt catgatctta agcccaaaaa cggtaatgta tcacaaaggc aagttttacc
    38941 cctttgattc catcaccagc gcattactct acccagggct gggttacgga ttgaacaaga
    39001 tccgctttgg gttggtgggg ctgtatctgc gcttcaccaa ccgctggcag cccatggaac
    39061 aaaccaccgt agccgagtgg atgaagaaat gggctggcga gaaggtatac actcagatgt
    39121 gggaaccgct ggtgatgggt aaattcagcg aaaaatacgc caaccaggtg aacatggcgt
    39181 ggatgtgggc acggctgcac gcgcgtacca cccggctggc tacttaccag ggtggttttc
    39241 aagcatttgc ggatgagttt gccgcgatcc tggcaaaaat gggcgtcaag ttcgcctacc
    39301 gcacggtggt gaaacaattg atgagcgaaa aagaaggtgg tctgacgctc tccaccactc
    39361 atggaaacga aacctatgac cgggcgctgg tgacaatatc accggcgctg ctgtcaaaaa
    39421 tggcgccaca gcttcccgag gattacctca aagggctgct tgacctgaag agcatgggcg
    39481 cggtggtaat gacgctcgcg ctcaaacatc aactttctcg cgaagggtac tactggtaca
    39541 acctgcccaa agacgcaggg tttccattcc tggcgctggt ggaacatacc aactttctct
    39601 ccccgcaaaa attcggcggc aaccacatta tctactgcgg tgattattta gacccggctc
    39661 acgagtattt tcagctttcc aaagaggagt tattacaacg ttttaccccc agcctaaaaa
    39721 agattaaccc gaacttcgaa acgggatgga tcacgggctc ctggctggtg aaaaccaact
    39781 acgcccaacc ggtgccgctg ctgaaccatt cccgtcatat cccttcaatc gagacgcccc
    39841 ttaaagggct gtattttgcc agtatgtcgc aggtgtatcc gtgggatcgc gggacaaact
    39901 tcgcggttga aatcgggcgc cgggctgccg acctgatgct gcgggcgtag ctgattgcgc
    39961 gctgatgaac caggcaagcg ccatacttaa agaccagatt tgaacctgaa aattaaaaat
    40021 tcctgatgcc aaaaaatgca ttatgggtgg gtttggcacg aggaaatgga aacctgtaac
    40081 ttttataata aggcgccata tagccgcagc gagggtaaaa accgcccatc atccccagat
    40141 gtaggggctt cggttgcgtt gataccgcag ggataaggat ataaataatc ggtaacgatt
    40201 gcgtaatcca tcaaagtccc caggttttct taaggttggg aaccttaaag atttgaattc
    40261 ttttaagtta gcgtagggta ttcgacttgt taccggggcg gctttctgtt agaatgctta
    40321 tacgcgttcg gacgaagctt aatccacagc ttcgggtaag cctttgtagg atccactaga
    40381 atatcctgtt cccaatccct gcttacgctg gagtggcggg ttctactgac ctaccccgtc
    40441 cgtgacgtga attccaggcg gaggcgcgtg ttggatcatc ggaacgcatg gcaggcagcg
    40501 gttggacaac tccaaatgga gatgtcgcgc gcatcttatg aaacctgggt acagcctgcc
    40561 gaactggtgc gttacgaaaa tgacctcttc acggttggcg tgcccaacgc ttacgcctgt
    40621 gactggctca aatccaggtt gaccgcaaaa ctcaacagta ttctcagcgg catcatggaa
    40681 tgccctcaaa aggtcgagtt catcgtctgg cataaggatt acgaaaacca gccgcagaca
    40741 gccagtaccg cggaaccttt accgttgatc tctcccgccg taacaggctc tcaaccggtg
    40801 gtgaaccatc gttataactt cgataacttc gtggtgggtt cttccaaccg gctggcgcac
    40861 gcggcctgcc tggctgtggc ggagaaccct gcccaggctt ataacccgct gttcctttat
    40921 ggcggtgtgg ggttgggcaa aactcacctg ctgcacgcca ttggcaactt tgcccaggcg
    40981 cgcggagagc aggtgttata cgtttcatct gaagaattca ccaatgacct gattaacgca
    41041 atccgcacac acaccacgca ggctttccgt gaccgctatc gtcgtatcga tgtgctgctg
    41101 gtggatgaca tccaattcat cgccggcaaa gagtcgacgc aggaagagtt ctttcatacc
    41161 ttcaataccc tgcacgggca ggataagcag attgtcatct cttccgaccg tccgcccaag
    41221 gccatgaaca cgctggaaga acgcttgcgc tcacgctttg agtggggatt aacggcagat
    41281 atccagccac ctgacctgga gacacgcatc gccatcctgc gctccaaggc ggagcggtca
    41341 aggcacgcgg ttcccccagc catcctggaa atcatcgcgc gccagttcca atccaatatc
    41401 cgtgaactgg agggcgccct gacccgcgtg gtggcctacg cggatctgcg cggacgcccg
    41461 ttgagcgaag aactggtcaa cgtggcgcta accgatctct tgccgcaacg gaatgctttt
    41521 gaaccggcac acgtggtgaa cgtggtggca tcggcatttg gcattacccc tgaaaaactg
    41581 atggggcgcg accgcacacg cgcagccgct ctacctcgtc aggtggttat gtacctgctg
    41641 cgccaggaag ccaacgtgtc gctgccgcag atcggcgaag ccatgggcgg gcgcgaccac
    41701 acaaccgtga tgtacgcctg cgaaaaggtg gcggatatga tcgaaagaga tgaccgtctg
    41761 cgccgccagg tgctgcaaat acgcgaacag ctttacggaa gaaaaacaac ctgaccgttg
    41821 tggtggtcag gttgtttttt tttccaggta tatcgaaatt aggatgctgg ctcttcgaaa
    41881 agcgctcgct taattttggg cagcagcaaa atgaccagca gcgcagtgct caacagcgcg
    41941 ccgatgaggt aatcgatcga agtagcatca gtgcgcagcg tgcggataat ttgcgtgggg
    42001 gtgttcatcg ccaccagtga gaccgccgct accaacgcca tccaccagcc ttccattttg
    42061 cgcgcggcaa tcttgtaaat ggcaacgaac agcaggatca cgcaaataaa ttgcaccggg
    42121 ctggaccacg ccagcaccca gccctcgatc ccgctgtaaa gcggcttttc gaggcgggtg
    42181 agcgtttggc gcatgccccc caccgcgatg acgaaggcgt gggctgtcat aatgccgcag
    42241 aaggtgagcg tgagcagctg ggcgatcttc ttctgcgtgt ttacttttct gagcaggata
    42301 aggcagagga agaacaacag gccggtaagt gatacaagca tcggcagggg cagcccaggc
    42361 acaaaactga cataaggcag catcatgaac attccgcctg aagcaggaaa agcggacgcc
    42421 agcagaccta ccggaaaagt ccattccttg ccttcgcgga tggagcgggc aatcacaatg
    42481 agcgtaatgc cggcgatgaa gatgatcgca cggtacagcg ggtagagcac tgaaaacaac
    42541 aggatgccgc tggcataagc cggcttttgg gcggaaactt tcaccagggc ggtcatcacc
    42601 cggtctaatg aggtttgaat aagaaacgga gccaccccca ccatgaacac ccccaccacc
    42661 accgccagga tggccaaaaa caaacgggtt ttactggaag tttgagacat attccctccg
    42721 aaaaaggttg attattctta tgtatattac ctgaaggaga aaaaaatacc ttgcgaaaag
    42781 ttaaacttgg tggaaaggaa acctgaataa tcggataaaa taaggtataa taaacgggta
    42841 attaagggtt cattatcaac aagcaggcta gttctggatt gaagaaccat tttgttcaaa
    42901 cattgcgttt ttgctctgct caagcagaga tgaagtgcca caaaaataaa ggaagtcact
    42961 cgcaaaccct ggcttgaaca accgccggga tttgtttttt atgaaccata tccgcggttt
    43021 taaggggcga cctttcgacg cggttattcc aatcaaaatt ccttaaatgg aacaggaaag
    43081 gtataaaagt aattaatgaa taagcaagat aaaaaagata gccaccgcaa gggtgcatta
    43141 aagatcatcc ccctcggtgg gctgggagaa gtcggcgcca atatgatggt gtacgaatac
    43201 gatgggcaga tcctggtggt cgataccggt ttgatgttcc cggaaaacga tatgctggga
    43261 atcgattaca tcatcccgga tatttcttac ctggtgcagc ggcgcaagca ggtgaagggt
    43321 atcatcatca cccatggaca tgaagaccac accggcgcca tccgccatgt gctggaagaa
    43381 ttacaggcgc cgatttatgc cactcctctg acgcgcggct tgttggaagt aaaactcgcc
    43441 cgcaatggag gcgcttcaaa agcgcagatt caaacggtca acgcgggtga gaaccgaaaa
    43501 attggcccgt tcagcgtgga tttcttccac gtgtgtcact ctattcctga tggcgtgggg
    43561 ctgggaatcg agtgcccggc tggactggtg gtgcacaccg gcgattacaa gtttgaccac
    43621 acgccggtgg acaactggcc caccgactac gccaaactgg cggagtttca gcgccgcggg
    43681 gtgctggtgc tgctggcaga ctccaccaac tccgagcgcc agggatggac cccttcggag
    43741 cgcgtcatcg atgacgcctt cgacaaagtt ttccgcgaag ccgacgggcg gattctcatt
    43801 gccacgtttg cctctctcat ctcgagaatg cagcaggtgg gcaacgccgc gcttaagcac
    43861 aaccgaaaaa cggctttcgc gggtctcagc atggtagata atgccaaaat tgcccgtgaa
    43921 ctaggctacc ttcaactgcc agataacctg attgtcagct tggatgaagc ccttcatatg
    43981 aaggacagcg acgtggtgat catgtgcacc ggttcgcagg gcgagccaac ttctattctt
    44041 gggcgtcttt cgcgtgggac gcaccgggtt ttcgatgtga agaatggtga taccatcgtg
    44101 ctttcctctc accccatccc gggcaatgaa gaaaacgtgt acggcacgat caaccgcctg
    44161 ctggagcgcg gcgctaacgt gatttatgaa gccattgcgc cggtgcatgt ttctggtcac
    44221 gccagccagg aagaaatgaa attgctgctg cacctgacac agcccgagta ttttattccc
    44281 atccacggcg aactgcgcca gctcaagcaa cacgcccgcc tggccaggca aatcggcatt
    44341 cccgaggaaa acatcgcggt cattctgaat ggacagagcg tggaactgag ccatggcaag
    44401 atgcgaatgg ctgagaaagt tccggcgtcg tacgtttatg ttgacggatc aggggttgga
    44461 gacgtgagcg acgcggtgat gcacgaccgt gagctgcttt ccgaagaagg cttgatgatc
    44521 gtgaacctca ctatggataa gtacacagac cgaccgaaaa aagagcctga tttcatcacg
    44581 cgcgggttca ttgcccagca ggacggcaac gaattgatga ccgggctgaa agcccgggtg
    44641 atgagcgtgg cggatagggc gaatggtcac ctgcagcaag atgttgaaca ggcggtttcc
    44701 aattacctgt accgtgaaac caggcgcaag ccgatggtgt tggtaaatat tattaaaagt
    44761 taagcaagtg aaatcaaaac gctcctcaaa ggagcgtttt ttattcgtgg tcgtcctgcc
    44821 agccgaaagc gccacgcagc ggcacaaatg ccaccggcag cgaaacctct ggcaaccaga
    44881 tgccattctc ctgctgccaa agttccagcc attgcacacc ccgaccacct acaggcagca
    44941 ccattctgcc ttcagggttc agctgtttca acagcggttc cggcgccttg ggggcgctgg
    45001 cggtgaccat aatggcatca tacggcgcct ggtcaaccca tcccagcgac ccgtctccct
    45061 ggtggacggt gacattcaaa tagccgagat cccgcaaggt atcgcctgcg ctgcgagcaa
    45121 gcgcgggcag atactcgacc gtgatcactt cgcgcgccag caacgaaagc accgccgcct
    45181 ggtaacccga acctgtaccg atttccagca ctttttcaga cccggtgagg tttaacagag
    45241 cagtcataaa ggccactata tagggctggg aaatggtctg gtcgctgccg atgggcagcg
    45301 ggtaatcagc gtaagcctgc tcgcgcatct ccagggggac gaaaaggtgg cggggcactg
    45361 tccgcatggc gttgagcact ccgctgttgg taatgccgcg cttctcaatc tggtggcgca
    45421 ccatctgctc acgttcgtgg gcgaagtcag gcggcatttg caagttccgc ttgaaccaaa
    45481 tctcgcgaga tcatacggtc tatcttcccc attgggtagc tctccagttc atccagcgcg
    45541 acccagcgca gctcagcgtg atgaagcggt ttggctgttt ttttttgaga gataatttca
    45601 taagcgtgca gggtgatgct gaagtgtgtg taagcgtggc ggtaggttcc aaacctgttg
    45661 ccaacctgag tagaaacacc caactcttcc atcaactcgc gggcgagcgc gtcagcgtgg
    45721 gtttcattct ctttcacttt cccaccaggg aattcccaca agccgcccag cagccccctg
    45781 gcagggcgtt tggcgatgag cacccggtca tcctggcgca agatggcagc ggtgacgacc
    45841 aggtgtggga cgggcttttt ttctttcatg agcggcagct cagcttgggc accctgttgg
    45901 aaagcggcgc aaatggacgg aagcggacag cggtcacaga gcggcttacg cggcaggcag
    45961 acgagcgcgc caagctccat aagcgcctgg ttgaaatcgc cagcgttccc ttctggcagc
    46021 agctcctggg cggcgtgccg cagcgcgctc tccgccgtgg gggtgttcac cgccagggtg
    46081 taaggcaaaa ttcgagccag cacgcgcttg acgttgccat ccaccaccgc gcggtccaaa
    46141 ccgaatgcca tggaggcaat ggcggcggca gtgtacggac cgatccccgg cagcttcagc
    46201 agttgttcat aatcggcggg aatttttcca tcatgcagct gcatcatttg ctgcgcggcg
    46261 cgccgcatgc accgggcgcg attgtagtag cccagacctt cccagacgag taacaccgcg
    46321 tgttcactgg cgttcgccag cgtggaaata tccgggaagt cgcgcatcca gcgcagatag
    46381 taaggcaaca ctgtatccac ccgggtttgc tgcaacatta tttcagatac ccagatggcg
    46441 taaggatcgg aatgcccgcg ccagggaagg gagcgggcat tttggcggta ccagtttaac
    46501 agaagccggg caagctcatc tttcatgggg tggatcacag ttgaaaacgg ttgcgcgcgt
    46561 agaccagctt catcgtgtaa ttctgaatga aatcctgcag gcggttgttc aaagaaagcc
    46621 agtcttcaga gaacaacaac tggcgggctt tacgcgggct gccaatgatc acgctcaccg
    46681 tgatctgtgg tttgttgccg taaacagtag cccaggcatc cgtaagctca cagcccatgc
    46741 gctgcacgcc ggggatgtaa tcacggatga caaactcaaa ataatcctgt tcgtgctcag
    46801 gcgtgatgtc ccacgacatg attaatttca caagattctg ttcttccacg gtcacgagtt
    46861 gttcatctgg tattgcagta tcaccacccg tgtctcatcg ggcagcgagc tgatcccggt
    46921 tacttttaag gttggaacag gctcaacatt ggagagcccc atttccttga gaaacctgga
    46981 gagaggttcg agtttttgcg gggttgaggc ggtatgcggg gtggcgtaat gcatagggat
    47041 gacgatattg ggctccaaca ggctgatcac ctcagtggct ttgatggcgg tcaggctgcc
    47101 cccggctccc accggtacca gcgccacatg caccgggcca atggcttcta cctccgcctg
    47161 gctgggtaca cggttgaggt cgcccaggtg caaaacattg atgccgttgt aatcaaagag
    47221 gtacatggta ttgcgcggct cattttcagc cttgcgggag tgcccattgg tttgaatgcc
    47281 ggtgatgaag accccgccaa tttcaaactc acccggtccg tcaatgaggt gagaataacc
    47341 tttcaccgcg ctgatgagat tgtgaccggg cgtattatgg ctgacggtaa cgacatcccc
    47401 tttgaccttt aagggctcat aaccgacctg cttacaatcg taagggtcgg tgaccactgt
    47461 tgccatactg cgttctgtta agcgaaaaca ggaatgtccg taccaggtga tttccatgca
    47521 acctccacag accgattata ccgcaaaggc tcgcaggagg attgccatcg aggagataag
    47581 ctcaggagcc aacgcgcact cgctggatgg taacccgggg ttgagttcca tgcgcacggc
    47641 agatgcagga aaacagcttt tttatctcaa tggtgcatca gcatccattt cgttcttggg
    47701 ggcgaacacc tgtttcattt ggttctacga aattcaacta cccgtattga cgagcgcacg
    47761 gttctgcggt gaccttctta ttgctttttt tacttaagtt tttgatcgat cttgttcatc
    47821 tcgttttgca gatactcgaa ctctttcgat agttcgtgca tttcgttttc catctgtttg
    47881 atgcggttgt acatatccac gatgatcttt ccgtcctgtg aggtgtcgtg cttctgaaac
    47941 gctttttcca taatggggtc cacgatgatc ttgttcacca catcggttgg gccttccagc
    48001 atccattcct gaacatcaaa gcctttttta gtaatgaatt tgagcatgtt gttcttcaaa
    48061 attcctttaa gccggtcgat tttttccttg ttgtcataat atttattttt caagttcgcg
    48121 gcttcaattt gcatctgacc gcggatgctt aatagttcct cccgctgggc aggcgtcata
    48181 cctgccggct gatgtgatga gggctcaact tgtggtgggg tgttctgcac cggaacatct
    48241 gcagtaaatt gcctgcccgc ggttgccggg ggagtatacc gagttggagc gccaatcttc
    48301 gagaaccctg ccggatcggg tattggagga tattgaactg ggatgttcgg tggagggctt
    48361 tgcgcgggtg gtgctggtgg agctggcgca gcaggctttt tcttgagcgc atagccaatt
    48421 ccaacactgg ctgcgcccac taggccaaca atgctgctga tgatggccgc gatcccccag
    48481 ggcgccacag tttttggccg tttgggctgt gcttcctgcg aggtttcagc gggctggcct
    48541 gtctgtggct gggtcagcgg tgctgcggct tcgccctggt aagcagggat ggtgaaggtc
    48601 tttgaatgac cgctgtgatc caggcacgtc cactgcagtt ccactgtcac aggtttaccg
    48661 tcccaaatca ttgaggagct ttcctgaccg ttgaagaacc aacgatcctg aatgacgctg
    48721 gtttcctttc ctgccgcatc cgcataactg gctcctggcg agatcgtgtc gcccggtttg
    48781 gcgggattca aaccgcgcac actgggtgtg cagcccgtgc ctttggttgg ggtggcaggt
    48841 ggggaagtaa aagcctcagg gttttggatc gtatatactg aggtcatttc ttggattcca
    48901 aaattgggta aatccatcac aaacagacac tcgatcgttt ttccatcgat tacctagcaa
    48961 gtggtaatct cttgataatt tcctttaggg aatgtgatcg tgccattggg tccgccggaa
    49021 aaggtgccaa ttaattccgc ataaccatcc acaaaacaag caagccctcc atccgggtgg
    49081 tttccgatat gggtaatcgc ataaacatag acagcctttg aataggcttc tcctccttga
    49141 gaacccacat tgaaaaattc aattcgattt tccaccccat tcgggaagca atcactatcg
    49201 tacaaggttg caatgacgcg ctgaggttcc acctcgccct gtgcttcgac atcgttgggc
    49261 agatacatca aaaaataaaa tataaagaaa aaggaagcga aaaaaaatga gagtagcgtg
    49321 cgttggatgc gagattccat gtgaacatcc tttcttgcac aacctcatta gaaagttctt
    49381 aatccctgtc tttattttag cacgtatcgt aaacagctaa cccctgtgcg cattcattca
    49441 aattggagac aaaagggaga caagggctga ctcacctcgt tgataagaat atttctgcgg
    49501 ggacgataga agggctccgg attcgggttt ttgctgccca gttatgctga gcatcagccc
    49561 agcaggcgct ttttttcctg ctggaaaata cgcctgagga tggccacaga agcggcgtaa
    49621 gtggagtcca tgccgcggta tgaaagagtg cccgcgccaa ggtttttccc cttgctcaac
    49681 ggcgtatccg aggcgtaatg caaaatgccg atatcaaaat tgacgccttg caggttgaca
    49741 atttcattca ccgggtggcg cttgggacgg gtcatctcgt acaccgctga aagatatgga
    49801 ccagcctcca tttcaatgtc ggtgtaaccc tcgcggtaca tcacatccat caaactggca
    49861 ttctgtaaga aagtccccag taccgtgagc gccttctgat tatcgagcac agtgccaaaa
    49921 accaaccatg gagccacatc aggggaggta aagacattct ggaaaaggta agtgttctga
    49981 gaatgctcgt cttgcaccac gccggggatc attatgtcac cgcgcacgcc attcagcgtg
    50041 gcggctttgc ccataatata caccccgagg atctctgaga tgtgttcggc gattttggta
    50101 agcacattgt aggcgccaag ccccagcggg taatctatgt tgacaatcag cgcgttgctt
    50161 ttttgcaaaa aaaccgtatt ttcaccatcc gacagcaggc gcgggtcgaa gtgttggggg
    50221 tcaagcctgt tgatctccac cacctgggct tcgatatcga aggagtgggc gggaggtacg
    50281 cgcgtgatgc cgtggcgctg ttcttcgcgc gccatttgag cgttttccgc ggcgccttgt
    50341 gcggtctgct ggtatttctt caacgcgtaa tagagaaagt tctcggttga agcgcagttg
    50401 ccgcgctcct ggatctcgtg ccattctgcc agtaattcct ggtttccctg ttgctgcagg
    50461 aaggtcaaaa tttgctcttt acgcgtgagc gcgtacccac tgagcaggtt gggaatactg
    50521 tgggtgttac tggagatgag atacaccggg cgctcactga acaaaggggc gctgcgctca
    50581 atattttgcc accacaggcg cgtggcgcgc cagtattcgc tcagcgaacc gctgagcaac
    50641 cggatgccaa aagaacaccg tctgctggcg atagtctcca ggttactgga aaagtcactc
    50701 ccccacactg ataccagccg ctgcaggtct tcgagagggg tgcctaaaac gtggctgagt
    50761 tccacgaatg atgggctgcc ctcaaggctg gtttcttccc aggaaggcgg attccagcct
    50821 tgcaggaact ggtgcatctt attccactct atctggaaag cggtgagggt tggcacaata
    50881 tcttcgatat cagaacggct ggcgatgaac accgccaggg tgctgtttcc atcaaaataa
    50941 ctgcttcgcc gccgcgcccg cgcggtaacc ggcagccagt tctccacttc agggtagcca
    51001 tggcgcgaaa acaactgaac gctctgaccc aacacgatag aacgcgttcg catgatgcag
    51061 gatggcaggc gcaggctgga ataaataagg gctgaaagat cgagagatac tttacgcgcg
    51121 tctgggtgca gcgacgaatt catgccggcg tgtacttctt ccagcgtgcg cacctgcacc
    51181 tcggtggtgg agcgcagtaa agagtaaatc gttctcaggt acaggtctat ttcatctgtg
    51241 gcgctctttg gcacagttct ttccattctt ttgttctcct ggctgtattg tagcacaggc
    51301 aaagcggagc aaaaacagtc agcacttcag cgcgatagcg ggctttcacc agtaacatgc
    51361 aggcgaactt ttgctatgct gatgcgcagt taatggaatt gttgcagcgc gtatggaagt
    51421 tcacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51481 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctcagt ggtttggtac
    51541 atttcagcca gcgcattgcg cgtgaccgat tcgggtggtt caatgtgacc gaaggtgttc
    51601 aacaccctgg taaggtaggt ttctactggt ctttcgatga ggtctatgtc acgtgattgg
    51661 taataatctg caagagcgtt tttaaaacgc ctgacaaact catctacttc aaacaggtag
    51721 ccctctcgtt taagcgcgcg tgccagggaa taatacgaat caagcaaaac agatgggtaa
    51781 tcaccctgga agtgaatcag cgtgaaaccc aaatcaaaaa aaaccgcctg cgttgtatag
    51841 gaggcgggga gaatgcccag gttcccctct atgaaattca tcaggtgttc accatctacc
    51901 tgatttaacc catccaaagc tcaatagtga cggtcgctga ggtattcatt caacaagggt
    51961 tccccccgta aatagcgctt caggttggcg gcgaacatcc tgccggcgcg ttccttgtaa
    52021 gcctggctga aacccgaaac atgcggggtg atgatcaggc gcggcgcttt ccagagcggg
    52081 tggtcggcag ggagcggctc cgtgctgaac acatccacag ccgccccggc taggcgtttt
    52141 tcgtttaacg cccgcaacag cgcttcttca tccacgatac ctccgcgtga aatgtgcacc
    52201 aggtaagcgc ccggcttcat ggcgttaaat tcttcttcgc cgatcattcc gcgggtttgg
    52261 ggtgtgagcg gcagggtgac gaccacaaaa tcgcattccc tgagcattga tttgagggct
    52321 tcgatagggt aaaggcggtt gaaataatca ccttctgggt ctccgtgacc atccatcacg
    52381 taaccgttat cctgcgggtg catgacatca cgcttaacgg cgagcacagt cacgtcaaac
    52441 gggcgcagca ggcgggctac ttcacgccca atgctgccat aaccaaccaa ccccacggtt
    52501 gagccgttta attcacgcgg tgagaatttt tcccagcggt catcgggcca ttcggctttt
    52561 tcctggtttt tcatcagtcc ccgcatatga tgaccaagcg ccaacaacat catcaggata
    52621 tactcgcctt cctgaatggc tgaagcaccg ctcatggagg ttaatttgag atccttgtgt
    52681 ttaagcagtt ctgacccctg caaaaagtca ataccagcgt agtgaaactg aatccacctg
    52741 agattgggag acaggttgag gtctggcagc aggatatccg tatagagaac ttcggagcgc
    52801 tgccagacct cgcgcggtat ctcgtcgatc ttactggcgg ggatgcaggt gattttgatg
    52861 cgtggagaaa gctcgcgcag ttgacccatt accgcttcgg ggaagggcac tgtggtgaga
    52921 acttcgatgt tttgcgctga cgccatgatg aactgctcaa gaacctttca ggtaaatccc
    52981 gcctggatcg agctcctcgc gcaacaactg aagctcagat ggcagtggag gttcagtggt
    53041 ggatacttgc ccggaggtgc gtacctgcca gccggtgttc tcccgcacct gttcaagggt
    53101 gcagcccggg tgcagggcgc tgagcacaag ttcgccgttt gcgtccggtt ccaacaagcc
    53161 caggttggtc accaccacca gcggcccgcc acccctggcg ccggtcagtt cgcgctgcgc
    53221 gcgcccgttc aggaatcctg ctgaggtgat aaaatccact ttttcgggaa agcggcgttt
    53281 ttgatgcggc gtgatgatat aacaccggtt ggcccagccg gcgatttcct gcgatccgcc
    53341 ggaacccggc aggcgaacct tggggtgttg ataatctccg ccgatgatgg tggcgttgat
    53401 attaccaaaa cgatcaatct gcgcgccgcc cagaaagccc acgtcgatgt tgccgcgctg
    53461 caggtactgg gaaaagatat cgaacatacc gcagacggat accgccccgg ttaccagcgt
    53521 ggggtcaccg atggaaagag gtaaccgcga aggccgggcg ccgataacgc cggcttcgta
    53581 aattaactgc aggttggggg cgtgggtacg gcgcgccagg ttgcacgcca ggttgggcag
    53641 cccaaccccg acaaagacca catcgccatc tcgcagcagg cgggcggcgt tgatgcacat
    53701 cagttctgaa gctgtataag tgagatcgct cataccattt ccaccaggtg ctaataatcc
    53761 ccgtagttta cgggagctgc gtaacgaggt tttaccgcca ggcgggcgtg agtttcgctc
    53821 cccagcttct gccagtattc ttccgcaccc tgcacgccgt gcacccattc atcgagccag
    53881 gcgttgaccg tcgcgcggtc ttcactgatc ttatcccagt tgagatagaa cacattgtcg
    53941 cggtcgtaat atccctgggt gtaagaggga tgcgcgcagt gcggtacctg gcagacggct
    54001 ttaacaatga gaccaggaat cagcgtgcgg ttggggtctg aacggatgac atcttcgctc
    54061 acgatttctt cagcggtgat aatgacatcg cgcgaagcga aggcggcttc tttttgttca
    54121 ccaaggatgc cccaaacctg cgcgttaccg tgttcgtcag cgcgctgcac gtggatgatg
    54181 ccgacatcag ggttgagggc aggcacggta accacatcct ggccggtgta agggtctttc
    54241 acgcggcgaa tattcggatt gctgcgttcc aggtctggag cggcggtctg attcatgggc
    54301 atgaaaggca ggccagcggc tccagcctgc aagcgcgaga tcatgccgaa atgggaatac
    54361 tcttcccact ccagacgccc ggcttcaagc tcttcgcgca caatgcgcag tgagcctacg
    54421 ccggggttac cgatgtaaga aaagattagc tttttcgcgc agccagctgc taccatctga
    54481 tcatagatca aatcaggggt cgcgcgtgcc agggtaaggt tctttttacc ctggcggatg
    54541 atctcgtgac cggcggcgaa ggggatgaga tgggtaaacc cggcgcaaaa gaccgtgtcg
    54601 ccatcatgaa cataactgga aatagcctca gcgagcgagc agattttgga catatcgctt
    54661 tttacacctt gcgaaccagg ttctggatct gttcgtgcat atagagttgc aggtaatgta
    54721 aaccgccggc attcttaccg ctggaacctg agcctttcca gccgccgaaa ggttggaagc
    54781 caggccaggc cccggtggtg gaacccattg gacggttggc gtaagtgacc ccggcttcaa
    54841 tattatcgaa gaaccacgcg gcttcttctt ctgtaccgta gaacccggcc gtcagaccgt
    54901 actccacgtc gttggcaatt ttcatggcgg tttccaggtc atcgaacttg ccgacggtgg
    54961 tgatgggtac gaacatctcg tacttccaca ggcggtgggt gtaaggggct tcagcgatgg
    55021 tcggcgcgca gaagtagcct ttggcgagat ccccatcggt gagcaccttg ccgccggtta
    55081 gaattttgcc gcctgccgcc agttcgccaa caaaaccctg gtagtcgcgg tagctgccct
    55141 tgttgacgac aggacccagg taattggcgc gctcggtggg gtcgccgatg gtaagcttgc
    55201 cggtcaattc caccaggcgg gcgaccagct ggtcgtaaac cgaagaatgg atgagcacgc
    55261 gcgagttggc agagcatttt tgcccctgca gcccaaaggc ggagcgcacg atgccaatgg
    55321 cggctgcttc aaggtcagcg tttttggtga cgatggcggg gttcttgcca cccagctcga
    55381 ggatggcagg gcgcacgtag cgaccctgag cgaaatcgcg aaagatcttc atgcccacat
    55441 cataagaacc ggtgaaggtg atcccgtcca cttcggggct ctcaatcagc gcctggccga
    55501 gcgtggagcc aggcccggtg acgtagttaa acacaccttc cgggagaccg gcttcctgga
    55561 agcattcagc gatcaggcgg gcggtccagg gggtatcggt ggcgggtttc atcaccaggg
    55621 tattgccggc taccagcgcg gcgcctgaag ggccgccggt gagcgcgccg gggaagttga
    55681 aggggctgat gaccagccac accccgtaag gtttgagcac ggaaacattg cggctttcat
    55741 agccgctgag cgggtcgcgt cccatagggg caacaaagcc atcgctggct tccatacgat
    55801 cgcaggcgta acggatgaga tctgcggttt ccgccacgtc tcccagccct tccatccggt
    55861 tcttgcccac ttcaagcgat aaaaccacac ccatttcaaa agtgcgccgg tccatgatgg
    55921 cggcggcttt gcgcaacaga aagacgcgtt cttgccatgg cgtatgcgcc catcccctgg
    55981 cggctttgcg cgcagcggcg agcgcggctt tggcgtgttc agcgttgcct ttttgaaagg
    56041 ttcccagcag aacgtcggtg ttggcgggcg agcggttatc gaacttctct tcggcaaaca
    56101 cctctttgcc atcgatcaac attccgtgat ctcgaccgag ttctgatttg accttggcga
    56161 gcgcctggtc ataacgggtg tgaagttcct cgggcgggtt gaacatggtg gcgtaagtga
    56221 gcttgaagac ttgatctgca gccatctgaa aatgcctcct gtataaggtc agagattgcc
    56281 cgtgggtttt acgcgggatg ttttaagtat agcaaatgag aagaaaggcg tcaaagtaaa
    56341 agcgctaact gagcccaaaa ggttatgact tccctttcaa gatccgcgct gacgaacgct
    56401 caattgaaca cagtacagat tcaatgaaaa cgagtttctc aaacgatggc gattctttta
    56461 taattatcaa agatcctgga aaagagggtg tttgatacct gcagcgcaac ctgtaaagac
    56521 ggcttttgtt gtcattaatc cggtggccgg cattgtcaac gcggcattgc tgcaacggaa
    56581 cattgaaagc cgttttaagc acaaaggttg gcaaacccgc tttcatctga cggcggcggg
    56641 tgaagatttg aagactatcg tgcagcagca aattctttcg ggcgtggacc tggtggtagc
    56701 ggcaggcgga gacggcactg tcgcggcggt ggctgcgggg atggtgcatg cgcctgtgcc
    56761 gttgggtatc atccccacgg gcacctggaa tgccatcgcg cgcaacctga tgatcccctt
    56821 tagcagcacg cgcgcggtta atttaatgac cggcaggcat cgcatcaagc agcttgatct
    56881 aatggcgatt ggtgaaacgg tgcacgctat gaacctgggg atcggattca gctcgatgat
    56941 gatcgaaaat accgcccgcg cggaaaaaag gaaatttggc agccttgcgt atttttctca
    57001 ggggtttaaa caaatttttg gcttgcaaca gcgccgttat atgatcgaag tggatgggaa
    57061 gcgctaccgc ggaagggcgg cggaaatttt tgtggcgaac tatggcatgg tggggttgaa
    57121 cgtgctcgaa gcatcgctgg atatcaagcc ggatgatggc aaggtggatg tgctcatctt
    57181 gcgggcgcgc aacctgtttg acctgcctgg gatgttctgg cagatgtttg ttcagcgcaa
    57241 gcgccgaacg cccaagtacc ggcagctctc cgcggctgaa tcgctgttta ttcgcactaa
    57301 cccaccagcg gctgtacagg cggatggcga gttgattggt aaaaccccgg tcacggtgca
    57361 ggtgttaccc cgttgcgtga aggtgattgt gccgcttgaa cctcccctct catttcccct
    57421 tcaaccgcga cgggtataac gctcccacaa caaaaaggtg acctccctga gccgttcacc
    57481 gagcgttgtt ccacgcgctg tggtcacgtc atccgaatta tcgaaaaccg cgtagggcac
    57541 acgccgaccc tgaatctcca ggtattccgg gtcagcggcg cttagctctt cccaatctga
    57601 ttggcggtag agcctttcca atttatcatc ggggataccg tgctcaagga tgctgtctgg
    57661 tttttctggg ttgaagcgca acctgttctt gcgcagtgat tcttcccaac ttacgtcaac
    57721 atacagcacc gccatacgct ccagcatatc cggggagagg tggcagaagg cagagcggta
    57781 tccaccgtgt tcactgccgc gggagaattc aataagcgtg gtgtattctc tcgccagccc
    57841 tggcacgtcg cgcaggcgct tttgatactc taaacagata cgctcgatca acaggttcca
    57901 taaatggttc cctaaaaaat acccggcgga atcagtatgc aggcgggggt agcccatgcg
    57961 ttccagcaac gcgtcttctt cgaaccaggt ccacaacatg gggaagtcat cgatttcttc
    58021 cagtttacca atatgaaagc gctgcaggcg ctggcggaga gtggttttct ttaaataatc
    58081 aatgatctcg ctcttgcccg ctgccgggcg ggcgatgagc agcaaaattt caaatcggtc
    58141 agcggcgggc atgttttcct cctgtagatg catcttgatg attgtgggcg agatgtgaca
    58201 tactgtcaag accccggttt tggcaaatga tataatcgag aacatcctcc gcgggggatt
    58261 ttttcaacca tgatcgaatc tgaaaccact caccgtaata aatggctcat cctgctggcg
    58321 atcggcatgg gcgtgttttt agccaccatc gacggtggga ttgtgaacat tggattgaac
    58381 acgctggtga aagatttcgg cacgccgttg gcagttaccg agtgggtcgt gttggcttat
    58441 atgcttacaa tctccagcct catgctttca attgggcggc tgggagacat gattggcaag
    58501 aaataccttt atattgccgg gctggccatt tttactgttg ggtctgtgct ttgcgggatg
    58561 tcggagacca tttactggct gatcgctttt cgggtactgc aggcggtggg ggcttcgatt
    58621 accatggcat tgggcacagc gatgattaca gaggcttttc ctgaccatca gcgcggcaaa
    58681 gccctgggca ttatgggaac gctggtctct attggtatca ttgccggccc aacgattggc
    58741 ggattgatac tggaacattt aacctggcac tggcttttct ttgtgaacct accggtgggc
    58801 atcctggggg tcttgatggt gtggcactat gtaccgcttg accgcccggg aaagaagcag
    58861 tctttcgatt ctttaggggc tgggttgtta ttcatcacgc tcagctcgtt gttattggcc
    58921 ttgtccagga tccagagcgc aggtttgacc gaccctttga tccctggcct gttattcctt
    58981 tttttgatcg ggctgattgt ttttatccgc gtggaaaagc gcagcgctga accgatgatc
    59041 gatctctcga tgtttcgcaa ccgtatgttt tctgtgaacc ttatcaccgg gttcttgacc
    59101 tttgtcgttg gggcaggaac catgattctc tttcctttat atcttcaaaa tgtattgggg
    59161 tttggcccac agcaatcagg cttgatgatg gctgtcaccc ctcttacagt ggctatcgtc
    59221 gctcccttct ctggggcgtt atcagaccgg gtgagttcac gggtgttaac ttccattggg
    59281 ttgggggtaa tgcttgtcgg gcatagcctg atttcaacgt tgagcacgga taccagtgta
    59341 acggggtacc tgctgcggtt tatcccgctg ggcattggca tcggcctgtt tcaggcgccg
    59401 aataacaacg cgatcatggg atcggccgcg agagaacgct ctggcgtggc ttcaagcctg
    59461 ctgagcctta cgcgcaccgt gggccagacc acgggaattg cgcttttggg ggcattttgg
    59521 gagaggcgcg ccaccacgct ggcaggagac gacgcgttgc acagcgcgct tgcggcgcca
    59581 cttaaggcgc aggtgggagg gctgcagcag accatgtacc tggtcattgc cgtcatcatc
    59641 gcggcgctgg ggctctctct atgggcgctt gctgcctggt tgagagagcg cagcccccaa
    59701 aacgcctgac cagcgaaccc agggagaaaa ggcagccatg agagcaagta taattaattg
    59761 ggttgattcc atttaaagac gaaaggacgc ttatgatgcg taaatatttg atttggatta
    59821 cgacgatctt catgcttgcc tcatttatgc tggtatcctg cagtctgcct gaggttaccc
    59881 ccccggcggc aggtgaaaca gcgccagtgg tctcgacccc cagtgagcag acagtgccac
    59941 tgcctgccca ggtgataaca gccacccctg ctcaaacccg gttgatcaaa gccagcgagg
    60001 ttggcaggtt ggagatttca cagcgcgtgg cggtgagcaa tatccaggat atttcctggt
    60061 cgcctgacag caacaccctg gcgctggtca ctcaaaatac ggatgctgcc cagaatcagg
    60121 tttttagcgt taccctgtta aacgcgaagg atctctccac ccaggccgtt tactctgcaa
    60181 ccggcaaccg ggtgacctcc gttgccgcgg atggaaaaac cgcagccgtg atcaaccagg
    60241 accagatgtc tttctcactg gtggatacca gtgtgaagga cggcgcttcg cgcagccgcg
    60301 ttaccgattt tctgattggc aacgtgacga tatcacccga tatgcgttac gtggcggtga
    60361 ccaaagctga attttgggaa gtgatccttt atgattttaa cacgctggaa gaggtgcgct
    60421 ttttatccgg gttctcaacg gctgcccctg tttttgacgc gggttttaac cacagcccac
    60481 aatggctggt gtggcacgcc cgcgccaccc tgcagctgca ggaggtggag accgggatgt
    60541 tgagcaacct ctactcgcac gaggatttcg ttactgctta cacgttatct cccgacggga
    60601 tgatccttgc cagtgcttcc ttcagatcta ccaacgacgc ttcagtacct gtggtgatcc
    60661 tttgggatac cttgaatgga acggaattga aggtgctgga aatcagccaa ccggtgaacg
    60721 ctctgcagtt ctcgccagat gggaaattgc tggctgtggc ggcagggagt gacctgcaga
    60781 tttgggacgc cgctggcgga aacttgctca ccacgctcac tgggcattca gatttcatcc
    60841 tgcaactggc tttttcacca gaccaacaca ccattgcttc tgccgggcag gataagcagc
    60901 tttatttgtg gcagattctg gaatagacaa ggtctgcatg actgacccgg ccataaacga
    60961 ccgggttttt tcttgaaata atccttgtgg tatactacca gcgactgttc caggctcagg
    61021 atcgcatatg ccgctgctga ccgctgccaa tctctccaag tcttacggtg ctctggatat
    61081 ttttccagag atttctttca gtattcctca ccgggcgcgc atcgggctgg taggcgccaa
    61141 cggggtcggc aagacgaccc tgttgcgcgt tctgatgggc gaagaggaac cttccagcgg
    61201 caccattcac aaggcgcgcg gtattcgcat cggttacctg ccgcaggaag cagctctgga
    61261 ttcgtcccgg acgttatggg gggagtgcat gactgttttt gaaccgctgc tcgccctaca
    61321 gcaggagctg cacgccatgg aagaggctct ttcagccaac ccggattcga ctgacctgat
    61381 gcaggtctac ggcgaacggc aaagggaatt tgagcacctg ggcgggtatg aatttgaagc
    61441 ccgcacccgc atcacgcttt cgggattggg atttaagcgc gcggatgaaa aacgcccggt
    61501 caggcagctt tcaggcgggc agcgcacacg ggtgctgctg gctaaattgc tgctttcaga
    61561 acccgagctg ctgctgctgg acgagcccac caatcacttg gatatttctg cggttgaatg
    61621 gctggagggg ttttttaaag aatggcaggg agcggtgctc attgtttcgc acgaccgtta
    61681 cttcttagac cagaccacca ccacgatttg ggaaatgacc cccgcgcttg aagagtatcg
    61741 cggtaattac tccgcatacc tggcgcaacg cgaagagcgc taccagcggc gactgcaaga
    61801 ataccaggct cagcaagaat ttattgaaaa agaagaggaa tatatccgcc gcaatatcgc
    61861 tgggcagaac acccgccagg cgcaaggaag acgcacccgc ttggaacgcc tgttaagcga
    61921 agccaggtta accccgcctt tgcagcgccg cacactgcgc atgcagctgg tttcttccgg
    61981 cagaagcggt gacctggtgc tgcgcacgcg ttctctcagc gtgggttatg cggatgaagg
    62041 caagcccttg tttgaagtgg atgacctgac cctcgttcgt ggagaatgcg ctgccatcat
    62101 tggccccaac ggcgcaggaa agaccacctt ccttaaaacg attcttgaac aaatcccccc
    62161 ctacgcgggg gaaaccgtat taggcgccag cttgaaggtg ggttactttg cacaggcgca
    62221 cgaagggctg caccctgatt ggacattaat ggatgagata caggcgatga tgccgaaatg
    62281 gctgccgggc gaaattagag actacctggc gaagttcctg tttaccggag aagatgtgtt
    62341 caagacggtt cacctgctgt caggtggaga acgagggcgc ctggcgctgg cctgcctggc
    62401 gttacagggc gccaacctgt tgctgctgga cgaacccacc aaccaccttg acctgccttc
    62461 acaggagatc ctgcagaaca tcctgggaca atttcagggc accattctac tggtttcgca
    62521 cgaccgctac cttattgacg cgctcgccac ccaggtgtgg gaggtgctgc cggaacagcg
    62581 caaactggcg gtatttgaag gcagttatag cgaataccgc gctttaaagc aggcaaacct
    62641 gatgatacct gatgggagac cgcagcagaa agtggggtta acccgggcga aaaaacccgc
    62701 tgtggggaaa agtaaagacc agttgcaacg catgaaggcg cgcctgcgcg cgctggaaga
    62761 agagatcgcc cgcctggaga tcgatgcgcg cggcgttgaa caacgtttaa gcgatagagg
    62821 ggttgatcct ggcgaggtgc agcagctcgc cagcgaatac gcgcgcctgc aggcgcagct
    62881 tgaagccaaa attgaattat ggagcgattt gggagatgag ttgagcgagc cataaaccct
    62941 ggcattcctc cggggtttat cagcgctatc gaaacaaccc gttcaggtgc cccaatccct
    63001 caggtacagg aagtcataac cgacagtgcg accgctcagt ttggcgatgg gcggcatgac
    63061 gcgtttgaac tggttcttgc gcacgcgctc gatgacagcg tgaacaaatt tcacctcaaa
    63121 tccactgtca acacattcct gcggcgtgta acgctgatct accagcaagt agagcagctg
    63181 atcaaccgca gcgtaagtga agcccagctc atcttcatct gtctgcccgg cccacaggtc
    63241 ggctgaaggg ggtttttgca gcagtggttc agggacaccc accgccgcgg cgagctgtcg
    63301 taactgggtt ttatacaggt cacccagggg gttgagcgca caggcggcat cgccgtgcat
    63361 ggtggtatac cccagcagaa tctctgtttt attgctggtg cccagcacca gacctttgaa
    63421 gacctcggat tggtcaaaaa ggatgatcat gcgctggcgc gccatcacat tccccctgcg
    63481 gcggtttccc atatccggat agcggttgaa caatggctcg accatatcgg tgattggaat
    63541 cgtgagtgtt cgcactccga gcgcgtcaat caccagctga gcgtgagcca gtgaatctgc
    63601 tgaggaggtg gcataaggca tacgcaccgc cagcacattc tctggttgta acgcttctgc
    63661 cgccagataa caggcgaggg ctgaatcgag accgcccgaa agccccagca cggcgcggct
    63721 gaaaccggca cgcgtgatct cagtatgaat gaagtcagtt agaatcttgc gcgccaggtt
    63781 ggtatcaatc gtcaggtcga gcatttgctc ctcctcgtgc ttcttcagcc atctgcatta
    63841 cgggtgataa tccgcgtgat ttcgcgctgc accaggctgg tgcgttcatc acgcagcagc
    63901 ggcaggcggg tacgcgtacg gcgcagctgg cttaggtcca gctcggccag caccagggct
    63961 tcggcgtgtt ccacaccatt caccaggggt tctccatctg gtgaaaaagc ggaagaaccg
    64021 ccccaaaaat ggataccatc ttcgaaacca gcgcggttcg tgtgcaccac gaaagtggtg
    64081 aacatgccgg catacgcgcg gatgatatca ttcacccaac gagcggattc tgttctacca
    64141 ggggcagtat tgtgaggtgt gggggtgata ccgcgcccag gagaggcgga agtaaaaaga
    64201 aaaaggtcag cgccatccag ccaaagcata tatggagaag aggcgtgcca gaagtcctcg
    64261 cagatgagca tgccgaccct gccgaaacgg gtatcaaagg cgcgcacact ttcgccgcgg
    64321 gcgaagaaac gaccttcatc gaaaaaccca taggttggca ggtaaacttt gtgatggata
    64381 tggcgcaccg ctcctccgga gaggaaggcg gttgcgatat agaaaaggtt gcgttcgtct
    64441 tcttctacaa aacctacctg aatatcaata tcctggctgg caacgagcaa tgggttgaaa
    64501 accgggtcat ctggcaatgg acgacaggcg acagttggtg taaggtcttg caaagcgtaa
    64561 ccggtgagag aaagctcagg gaaggtgatg aggtcagccc ccgcgcgcct ggcttgttcg
    64621 attaagtcca ggtgtttttc caggttctgt tctacattcc ccagcacggt gtttatttgc
    64681 gccatcgcca cgttcagttt catacatttt cctctgactt cattttatcc tttttttttg
    64741 atcagatgtc agcaacaaca gcacagacag aagcgcgaaa gccaccgcga agattaaaga
    64801 cagaacccca tcgctcctga tacctcgaat cgggaaaacc acatctgccc tgaaaagaga
    64861 aaaaacaagc gtgtgggtaa aaaaaaccag gctgaccagc gaaaaacgaa aaccgggtcg
    64921 tttgaattgg gtgatatgtt caatcaaacc cagcaagagg agcaaggaaa gcgcggctac
    64981 cagctgcaga ggaaagcggt tgctcagcat accagtttca tcaagggcgg ggtacgaaag
    65041 aaatgcgccg gaggggagcg tctgaccgta ggccacgcct tcataccaca gacccagcca
    65101 gatgagcgct gccgtaggca gcagcatgcg gctggtgcta tcaagcgctg acagtacatt
    65161 caggcgcagc gcagcactgc ccaacagagc gaaaaacaca gcgccagcga acgcgccaaa
    65221 cgcgttgaac ccgcccagcc agaattgccc cgcctgttcg gggtgcagcg agtagtaaaa
    65281 gggatgagcg agcacataac caacccgggc gcctgtgagc actcccagca gcgcgaacac
    65341 ggcgcaaatc cattgcctgc tctccacacc agcgggaatg gagcggtaaa cacgccacag
    65401 accgatggcg gcgccaaaac cagccaggac ggaaaacaag cgtaatgtga gcatgtgagt
    65461 ttggggttga ggagctaacg ctgtggcaaa aaaatgaagc ccgtcattcg atggcttcta
    65521 cccctgtgat ttctgggaag aactggcgaa cggtcccctc gacccagcct ttaatggtgg
    65581 cgggcgaatg cggacagcca acacaagcgc cgcccatgcg cacttttacc actttcccgt
    65641 cgaaagaaac gaactccacc tcgccaccgt ggtaggtatt gatatatgcg ctcaattgct
    65701 cgatcaaacc gcgcagtagc tcaagctcag aataagaaca gcaattgtca gacatcatca
    65761 ctcctcaggg tttttacctt ccggacaggc tatcacggcg cgcagcatgt gcatgatctg
    65821 gtcgtgcgcc ttggccggct gctgcgccac agacatggcg attttttcca ataataccat
    65881 gcctgattcc tgatgttgtt cgcaaaaacg ctgaagttta taagcggcga tacggtatac
    65941 ccgggagtca gtggtggcaa acactgagga ggaataggtc ttccggccga gaatggaaga
    66001 ccaaccaaac acacccccgg aagtcaattt gccaatggtc agctccggac catcgtaagg
    66061 cacgtgggtg atttccactt ccccggttac caggatgaaa agatgaactg cttgctgact
    66121 ctgacgcaga atggtttcgc cagcagggta ggtaaaaacc tcgatgatgg atctcaattc
    66181 atcctgttca tccagcgaaa aaccctcgaa aatctgtaca ggcagcgggc taccattaag
    66241 catatttaca tcatatcgat tgtatgatat gccagatagt ggagaatttc ccttatatct
    66301 tgataaacct caccccgtaa aatgggcagt gatgatacat tgaaccctga ccttttttgc
    66361 aagaacaact tgaccgtagg gcgctcgatt cgaaaagggt gtcgtttttt cccgctgtgg
    66421 cagggttcaa gtacgtttgg gtaaatctat ggtgtggatg aaacgaattc ccacgtagag
    66481 gaaggcgggc ctggtacagc aataaacaag gagttccttc cctcgcgctg gtgtcggctg
    66541 caagctgaat ggtttacccc ggcaccacct tctggccggg agttccttca tgcattgcct
    66601 ccacgtcgtt cgtttttagc tgttgtttcg tgcgggagta tgagcgggga tgaccagggt
    66661 gtagccgtgt tcttttagca cctccagcgg caggttatag gcgacgctga gcaacccggc
    66721 atttaacacc tcttctggtt tgcccaaggc tgccagcttt ccacccacca gcaggccgac
    66781 ctggtcagca aaacgagaaa caaggttaag gtcatgaatg gcaacgacca ccgttttggg
    66841 ctgcatccct ttcacaagct cctctttcgt gggatgcgcc aactgatgga tgcgctgcat
    66901 caggctcacc tggtactgga ggtcgaggtg cgtggtgggc tcgtccagca gcaacagcgg
    66961 cgactcttgc gccagagcgc gtgccaggaa aagccgctgc tgctctccgc cagagagctc
    67021 tgaaacggcg cgcgagcgca gcgccagcag gtccatcttc tccagcgcct cttcaatgat
    67081 cagcatatct ttcccgttga ccttgccaag ccaatttaaa tgaggtgtcc ttcccagcgc
    67141 caccacttct tcagcgctga acgccggcgg gatgctgcgc gcctgcggta ccaccgcgat
    67201 aatgcgcgcg cgctgctgcg gagacaggct gtagatatcc tgaccagcca cttttactga
    67261 cccggcgcgc aagggtaaaa cgccgctgat ggcccgtata aacgtggtct tgcctgagcc
    67321 gttggggcca atcagcgcca gcacacttcc ctggggaagt tgtaatgaga tatcctcaag
    67381 gacaagctcg tgatagtaac ctgcgctgag cgcgttaact tccagcattt ttttcaccaa
    67441 tagttctgtg ctttcgcgag ttttagcacc cagagaaaaa atggcgcgcc cagcagagcg
    67501 gtgacaattc cgacgggtat ctcttgaggt gcgataagcg aacgcgccac cacgtccgtc
    67561 agcagcaaca ggctggcacc gcccagcatg gagaggggga tcagccgccg gtaatcggtt
    67621 ccccaaagca ggcggatgat atgcggcact accagtccca caaaaccaat gatgccggca
    67681 aaagctacag cggcagctgt tgaaagcgtg gccagggcga tggtgatcca gcgcgcgcgt
    67741 tgcacccgga tacctaattg cagcgcctgt tcctctccga actgcagcac gttaagcggg
    67801 tgaccgatga gcaccaaccc ggcgagcgcg atgagcatgt acggcagcac agcgaggatg
    67861 ggtgaccagc ctgtcattat tgcgccgccc atcagccata ccagcgcgcg gcgcagctcg
    67921 ccagtggaat ggatcatcaa cgcggaggtt actgccgagg tgaacgaact caccgccaca
    67981 cccgccagga tgaggttggt agtggggacg gttttaccca cacgcgctaa cagggtcaca
    68041 atgagcacgg tgagcagcgc gccgcaaaag gctgccagcg gaacggaaag cagcccgccc
    68101 cgggtataag gcgaacgtgc cagcatggcc agcacggccc ccaacccggc gcctgaagaa
    68161 acgccgatta aaaacggatc agccagcgga ttgcggaaca acccctggta ggcggcgccg
    68221 ctgcctgcga gcgccgcgcc caccatcgtt aacatgagca tacgaggcag gcgcaggtcg
    68281 ctgataattt tcaggtaaac gccgttcaca tccagggttg aggtcgcgcc gcgcagggcg
    68341 gatgtcacca catcccacag agtcggtaga gggatgaaca cgctgcccac cgccacactg
    68401 agcagcgccg acgcagcaag cgccagtgcg aggaccagga aaggtgagcg cgtgttcttt
    68461 ttacgcatca ataaagctcg gggtgaacca acctggccaa ttcttccaga ccgtccacca
    68521 gccggggtcc ggggcggctg accaggtcat cattgaaggg gtaaacccgg ccctcttgaa
    68581 ccgcgcgcaa accatcccaa cccggtcggg cagccacctg ctcaggggtc atgccgtaga
    68641 tggaatcgcc gaggaggatg atatcggggt tctgcaccag cagttcttcg atgctgatct
    68701 gcgcccattg agattgcatc gccgccgcgg cgttacgacc gcccgcttcg ttgatcagaa
    68761 gatccagaaa ggatcctggt cccggcgtcc agggtttggc agggtcagtt ccgtccagct
    68821 cataaaacac cactggtttt gtctccgcct tttttatcgc ctgttgtacc agttgaactc
    68881 tttctttaag tgaggaggtg agcgccgcgg cctctttcgt gtgaccgctg agcgcgccta
    68941 ccgtttgcag caaaccgtaa ataccctcga ggtcagtggg gttggagaga tagtaaacct
    69001 tcacccccaa gccctcaagt gcgctcacct gttcaggggt attgatctcc gcggcgatca
    69061 ccaggtcagg attaaggctg gcgatggtct caagtgaata gcttcccatt gaaccgccaa
    69121 catcctggat ggatgtgact tcttcagggt agttggagaa agtgtcgcgc cccaccacct
    69181 ggctgcccgc gcccacggcg aacagtattt cagtgaccga gggagaaaga gaaatgatac
    69241 gggcggcggg tttttccagg agaacctcgc gccccagtcc atcttgcagt tggatcttgc
    69301 cgccgtcctt tgccgcgggt atggcggaac aggcagccag catcagcaac aagagaaaaa
    69361 cagcgattcg tataattcgt gcgtgtttca tttggtcatt cacctctact atgattagat
    69421 ttagtaaaaa aacccaaccg ctagggttgg gtttcgcgtg tattgctgcg agtgcccacc
    69481 tcctttcagc gagagcgtgg taacaccctt tttggcggtc gacctggctt atcaggaggt
    69541 cctgattcac agttgcgcga cagcaccgga atatgcaccg gtttcgcctt tgagccgccc
    69601 tgttcgggtt aggcaccaaa aaggtcagat tgattactaa ggattctacc cgtgattcaa
    69661 agaagggtca agtgattttt aacaggtgat ggggcatttc aaacaaccgg tcaatcacag
    69721 ggggcggctg tataattaat gtatatatgg aggagccgtg acacgtgtac cttacgaaat
    69781 tcgagtgggt gaattgttaa aagagaaaca gaagaagctg gtgctggcgg aatcttgcac
    69841 cggcgggctg gtgggaaacc tgattaccaa catcgcgggc tcctcggatt attacctggg
    69901 cagcgtgacc gcctacgctt acgaggcgaa agaagcgctg ctgggcgtac ggcacgaaac
    69961 gctgctgcaa tatggcgcgg tcagccgcca gacggtgctg gaaatggctg ccggcgcacg
    70021 gcgctcgttg gcgggcggca ttccgctgga ttcaattatc ggggtctcga tcagcggtat
    70081 cgccggaccc ggcggcggac tgcccgataa accggtgggt accgtgtggt ttggcatgag
    70141 cacagcagat ggtgattggg cctggcgctg catgttcaaa ggcaatcgtg tgcagaacaa
    70201 gcgctctgcc gcgcgcaccg cgcttaaatt ggtgattgcc tacctggcgg gcagcctgcc
    70261 gcctgaaacc agatcacagc gtcattaagg gtgtccgatg gctaaacgac tgatcaggat
    70321 actcatctta accgcggatg ccggttttgg tcaccgcagc gctgccaacg cagtagccaa
    70381 agccttgaag ctcaagtatc aatccgcggt tgaagtgagg gtagaaaacc cgctggacct
    70441 cgagagcgcg ccatttttct tgcgtgaagc gcaaacagat tatgaccgct gggtaaaaca
    70501 ggttcccgag ctttataaac tcggttacca ggcgagtgac gcagcgattc caacacgact
    70561 gcttgaagat tctctggccg ttctactgtt tgaagcgatt cgcgacacat tttctgggcc
    70621 actgcctgat gttgtgctca ccacctaccc tatgtaccaa tccgcgatca tgctgcttta
    70681 ccggttaaga aagatacata tcccgctttt taccgttgtg actgaccttt ccaccgttca
    70741 ccgattgtgg tttaaccgca acgtagacgg ctgcctggtg cctaatgaga ttgtcgccaa
    70801 cctggcggca gtgaatgggg tccctcagga taaagtgcac atcactggca taccggtttc
    70861 accagatatc ctttatgaaa ccagaccggc agagaaaatc cgcgctgaac ttgggtggca
    70921 ccccgagctg accaccattc tggcggtggg cagtacgcgc acagaaaaat tgctggaggc
    70981 ggcgagcgtg atcaaccact acggctcaga attacagctg gtggtggtcg cagggaaaaa
    71041 tgaagagctc taccaggagc tcaaccagat cggctggcac atcccggtac agctgtttga
    71101 ttttgtggac aaagtaccgc tcttgatgaa ggcatctgat ttggtcattt gcaaggcggg
    71161 aggactgatt gtgacagaag cgctggcctg tggattaccg acggtactca cagatattat
    71221 cccgggacag gaaacgggca acgctgaact tatccagagg ctgcaggcgg gcgtggtggc
    71281 cgaaaccccc ctgatgatga tggaagccct gcatcatttt ctgatgaatg atcaaaaaat
    71341 gcttcggcaa tacgctgaca atgccagaac cattggcaaa ccagcggccg cctgcgatgt
    71401 tgcggaaatc ctttatgaag ccggaaaggc gagcactcct ccgaaagcgt cttacagagc
    71461 gcgcggtttc cgccctgctg agcgaccctt ggagggcgat agcgaatgaa ggagaatggt
    71521 gaggtagagg tcgccatcct cctctcggcc ctctctgagt ggcaaaccgc gcagtctctc
    71581 ttctcagata ccgggaatga aaactcacca tttggcgatt attttttaac ctgcgttgca
    71641 gaaaagcgct gtgtcttttt ccacggaggc tggggcaaga tttcagccgc ggcttcaact
    71701 caatttgtga ttgatcgctg gcagccgaaa ttactggtca acctgggtac ctgcggcggg
    71761 tttgaaggac ataccgctgt cggcgaggtg gtgctggtag acgagacaat ggtctatgat
    71821 atccacgagc ggatgaatga cccgtttgaa gcgcttcagt tctataccac gcgcatcgac
    71881 ctgtccttct tgcgcaggcc ttacccgcag gctgtgcgca tcggtcgttt ggtatcagcg
    71941 gatgaggata tcgacccggc caagatcaac cgcctgcgcc aggagtttgg cgcggtagtg
    72001 gctgattggg aatccgctgc catcgcatgg acggctcagc gcaacggggt gagggtgctc
    72061 atcctgcgcg ctgtgagcga cctggtgagc gagagcggcg gcgaactcta taatccaaag
    72121 agggcatttc cccgcagctt gctgcgaaaa atagtaagat
//
LOCUS       DAOTLW010000004        66432 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_479_length_66432_cov_9.629646, whole genome shotgun sequence.
ACCESSION   DAOTLW010000004 DAOTLW010000000
VERSION     DAOTLW010000004.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 66432)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 66432)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..66432
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_479_length_66432_cov_9.629646"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            53..355
                     /locus_tag="P5516_01220"
     CDS             53..355
                     /locus_tag="P5516_01220"
                     /inference="COORDINATES: protein motif:HMM:TIGR03831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YgiT-type zinc finger protein"
                     /protein_id="HRT91089.1"
                     /translation="MAESSLSNIDPVFPCRECQAGHMHRDYIAYFTWLGDELISVPDF
                     PAWVCDICGRCEYDENALTRLTLLLSPNAGKTIAPARLPRTKTRVKPKVQSPTQPD"
     gene            complement(383..2503)
                     /locus_tag="P5516_01225"
     CDS             complement(383..2503)
                     /locus_tag="P5516_01225"
                     /inference="COORDINATES: protein motif:HMM:NF012298.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HRT91090.1"
                     /translation="MTDIDPGFILKSRYKVLDLLGKGGMGEVYLAEDQSLANQVAVKV
                     NHNLSPNASAQFIREARLLASLKHPNLPRVIDYFTENDSQYLVMDFIPGENLRTIVEQ
                     KTPLSTPLILQWAEQLGNALSYLHSQNPPIYHRDIKPANIKITPKGEVILVDFGIAKT
                     GEASQETLSGAWGYTPGYAPPEQVSGLRTGPYSDQFSLAATLYYLFAGKPPADSAKRM
                     IGEEELNPLTQLNPTIPAHTASAIEKALSIKPEARFSTVQDFIVSLCNTSPIPDPSSS
                     QNTLVGQRTVAAVGRGAPLVPPPSVAPPAQVAPALPAKKKSPLGWIIGALAVLGLGIA
                     AVLVLRNLSLRGTSSNEPPPTLTPMVIVITAQSPETPAPATEEPTAAQNIEPTSTAEP
                     LPAETEAPVYTSIGEGGKVAFVSNRQADGFDQIWLMDVMQNESGQLVGVNPVQLTFSE
                     GDKANPAWSPDGSKLLYSGFSKGFTANGNPLAMDIWLLDLSVPDAQAVDISLRQRDDK
                     YPSWSPVGDMIVYTSYYRDDGIPQLFMTDPQGEKQTRLSLPFAESYATWTPNAEFLLF
                     VQSTGNLNVVYMRDKYSQYTNGEHKFDRMSDEGRLGSVSQPNLSLDGALLAYTQTSGS
                     KTNIYVAPFADRGKTITALTSSGKDSWPCWSPDGQWLLFSSTRDGNAEIYIMDKNGEQ
                     VTNLTNLASQDKEPAWQPVPFPIP"
     gene            complement(2743..2952)
                     /locus_tag="P5516_01230"
     CDS             complement(2743..2952)
                     /locus_tag="P5516_01230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91091.1"
                     /translation="MFRKTSGLIGMIAGLVIVAVSLLADTLGIGTYPGLNNVQLAGIL
                     VGVAFFVIGYLLRRKKSKEKKPAAK"
     gene            3360..4355
                     /locus_tag="P5516_01235"
     CDS             3360..4355
                     /locus_tag="P5516_01235"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003796 - lysozyme activity [Evidence
                     IEA];
                     GO_process: GO:0009253 - peptidoglycan catabolic process
                     [Evidence IEA];
                     GO_process: GO:0016998 - cell wall macromolecule catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH25 family lysozyme"
                     /protein_id="HRT91092.1"
                     /translation="MGLKYNDFAIRGIDVSEFNGAINWSKVQGHFAAIRVGHGRVIDK
                     RFNANWAGAKGKVDRLAYWYMDYYSHKDKSTSAYGISDKDWGRVQAEKCWSALKNDPE
                     GMVFLDIEKSSYGPALSTVQPRVLAIAQSFFDHYDKFSGKLNGIYCSLSMCTYFNEAL
                     RKRPLWVAWYNEAQNIDSVKKAVAARGWLGKCLIWQYASDGDVNDDGKADGTSLGMAY
                     GFLDLNGWLGTNAEYQQLFGKKAEVELYKVKILIYNLLVRNGPGLTYNRLRRADYPGE
                     YSIYEERDGYGRISQTLSEWISLDPAYVKKLGAAAVELTDAEKLARLWAAHPELH"
     gene            complement(4434..4505)
                     /locus_tag="P5516_01240"
     tRNA            complement(4434..4505)
                     /locus_tag="P5516_01240"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4471..4473),aa:Gly,seq:tcc)
     gene            complement(4585..6393)
                     /locus_tag="P5516_01245"
     CDS             complement(4585..6393)
                     /locus_tag="P5516_01245"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRT91093.1"
                     /translation="MTAQTAPRWNLTNVYPSLSSKEYNADCEKVKSFISDMESLMEKA
                     AAFSGQEDASTLGALAQEMIACYNAILLVGGTIRAYIESFVSTDSYNQEALRKESEFD
                     LVAVKINQLETQLLGWIGKVKERLPEMIQSVPVLEEHRYFLEESARQSRYLMSEAEEM
                     LAAELSLSGSNAWTKLQGMLTSQASVEIEVDGELKRLPSPALINLRSHPDGETRKRGY
                     EAEDKLWTSLKVPLSAALNSIKGEVNVLNRRRGREDAVHSAIDAAHIDRQSLEAMLGA
                     MRDSFPMFRRYFKAKVKYLGKEKLPWWDLFAPVGEVKSDYSFKEAREVILRNFGRFAP
                     QLEAFARNAFEHNWIDAEQRDGKRGGAFCMEVPGVGESRILSNFDGSFDQVSTLAHEL
                     GHGFHNDCAVKAGKTEIQKFTPMTLAETASIMCETIVTSALLEEVTNPQQELAILESQ
                     LNGDSQVIVDIYSRYLFEKEVFERREKTELNADEICEIMENAQKATYGDGLDERYLQK
                     WMWTWKPHYYSGGLSFYNFPYAFGHLFATGLYAIYQQRGAEFVPDYIHLLASTGEAPA
                     ADLAARFGIDIRTRKFWEDSLRVSEKRVERYCELVS"
     gene            6537..7202
                     /locus_tag="P5516_01250"
     CDS             6537..7202
                     /locus_tag="P5516_01250"
                     /inference="COORDINATES: protein motif:HMM:NF022441.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="head GIN domain-containing protein"
                     /protein_id="HRT91094.1"
                     /translation="MSEQSFNQERREVSGFSALVFRGMGRIDLLQSDHEELVIETQPE
                     IRSRIKTEVREGTLYIDYDENWKDWTGLRSLSGDKIVFKLMMREVTAISISGVGSLDT
                     PRIESANLSLAITGPGLLTVGTLKVPTLTVNLSGVGAIDVAGQTDELSVTLSGAGSLK
                     ASRLEAGKASVRLSGVGTATVWAKNSLETSISGAGVIEYYGSPQITQNNTGLGVLKFM
                     GNR"
     gene            7241..7834
                     /locus_tag="P5516_01255"
     CDS             7241..7834
                     /locus_tag="P5516_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HRT91095.1"
                     /translation="MAQDRTRKIVVTAVLGAITILLGLTHWGFIPWFGGISLTIMHVP
                     VIIGAILEGPLVGAGIGLIFGLFSMLQAAIAPTGPLDPLFTNPLLAVLPRLFIGPVAW
                     LVWTALKKWPVVGLIASGIAGSLTNTVLVLSVLGFFFGRAPVMTQIFGEELWKPLSGI
                     AVVSGLPEAGVSALITLLVVAAYRQFSVGKRKGANLE"
     gene            complement(7864..8685)
                     /locus_tag="P5516_01260"
     CDS             complement(7864..8685)
                     /locus_tag="P5516_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRT91096.1"
                     /translation="MSEFDFLSRIPLGQYIPTNSIIHRLDPRAKIIIFGVLLIAITFT
                     PSQLGLSIGIAALLLALLLSKVPIGFALKGLLVPLPFLLIIAIIQVFFYSSALDPNIL
                     IKLGKLNITSGGLLAGVMLLIRFAGLILCLSLVSFVTSTSELLHGLTKILSPLNRVGI
                     QTMDFVMIVQVTLRFLPFLAQSAERIAKAQASRGSDWGQKRKGLFNRIRQVIPMIIPL
                     FITSLRRSENLALAMDARAYGVLKYRSSMTELHFSFRDGLAVLISVVVSLAVFFA"
     gene            complement(8678..10417)
                     /locus_tag="P5516_01265"
     CDS             complement(8678..10417)
                     /locus_tag="P5516_01265"
                     /inference="COORDINATES: protein motif:HMM:TIGR04520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter ATPase"
                     /protein_id="HRT91097.1"
                     /translation="MPPLIEFDHVTYRHPGSEHSSPPALNDISVKIHQGEWVAIVGAN
                     GSGKTTFARHCNALLLPSHGQVLIAGQNTALKSNLPKIRAGVGMVFQNPEDQIVATLI
                     EEDTAFGPENLGVLPAEIRARVDAALETVKMSGHKEQLPHLLSAGQKQRVALAGVLAM
                     QPECVIFDESTAMLDPRGRLDVLAEMQSLHNRGITVLFITHFMEEAARAERVLALNAG
                     NLVFDGAPQDLFADLKLLTACGLEQPSTVMLLREIRQLNAQLNHLPVDFEQALAQIPP
                     YRGSHQLLSERLSPMTGEILIDVENLSHTYLPDTPLSHQSLFDVTLQVHSGAAHGFCG
                     ATGSGKSTLLQHLNGLYRPQSGKLRIGPYDFRDPRLDIKAVRRFAGLVFQNPEIYFFE
                     QYVGDEIAYGPKNLYGKENLAKRVREAMQLVGLDFDKFKDRLTYTLSGGEKRKVALAA
                     TMALQPRLLILDEPTAGLDPASRRNLHGILKDYQESGVELVLSSHSMGDITELTENLT
                     ILSKGKALRTGNTADLFNDARLIDEASLGQPSIVKLAGALREKGWPIPQRVVRAEQLF
                     QALRAVNTGGPHE"
     gene            10599..11384
                     /locus_tag="P5516_01270"
     CDS             10599..11384
                     /locus_tag="P5516_01270"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT91098.1"
                     /translation="MKRLIYLAVVLVLVFALAACSASSDHLAAIKKAGKMVVGTSADY
                     PPFEFVDEAGNTTGFDVDLMNEIGKRLGVKVEWVDMPFDSLIAAVQGGKIDLSIAAFN
                     YDEERDKTVDFTDAYYTSEDALVIMESLAGDPPTPENLEQFIIGVQSGTTQDAWLTDN
                     LEATGKLKAGSLQRYERVDQAFMDLQAGRVQVIMSDYVPAKALVDKFGGMKIVYQGVL
                     STGPMNIVIPDGDKHLQTEVNKIIKDLQDEGFIDALAVKYFSE"
     gene            11514..12149
                     /locus_tag="P5516_01275"
     CDS             11514..12149
                     /locus_tag="P5516_01275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRT91099.1"
                     /translation="MKFLQYIASGTAYTIAVTLVALPSGLLLGILFSILNVYGGKVTQ
                     RLTSLYSTIMRGIPPIVLLFILYFIVSGSINLSPFWAGSISLGIVSSAYQMEIVRGAI
                     LSVSGGQMMAARAIGMSKNQAIRHVILPQTLRLAIPPWSNEAAIVVKDSSLVYALGVP
                     EILRRAQLISASTQEPFLAYAVAALIYFLLVFITNRLLDALDRRLHIPANP"
     gene            12232..12927
                     /locus_tag="P5516_01280"
     CDS             12232..12927
                     /locus_tag="P5516_01280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRT91100.1"
                     /translation="MDLVVNEGDTIVIIGPSGTGKSTLLRCINLLTIADEGQIWLDDI
                     EITAKSCDEDKVRQHIGMVFQNFLLFNHLTALENVMLGLTKVRHMKPDEAKHKALLEL
                     ERVGLLDRADHYPGQLSGGQQQRVAIARALAMDPRIMLFDEPTSALDPELIGEVLEVM
                     RKLALEGMTMLVVTHEMGFAREVADRIVFLEKGSIVEEGSPEDLFYHPKHERTREFLW
                     KITELYGKKEKES"
     gene            12928..13608
                     /locus_tag="P5516_01285"
     CDS             12928..13608
                     /locus_tag="P5516_01285"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRT91101.1"
                     /translation="MIAWLKLFERYLPEFIKGAGVTLELTAVGLTLGFVLGLALAVAR
                     VYAPKWLKTIAIGYIELFRGTPLLVQLFIIYYGLPSLGITLSQTLSAFLALGLNSAAY
                     QAEYLRGAILSIGASQMTAGRAIGLSRWKTIWYVILPQALRLVIPAWSNEPISLLKTT
                     AIVFLIAVQDLMAKAKRAAADTYNPVGSYLAVAVVYLVLVFIFTWGLKYLEKKTRIPG
                     FEMGLKKG"
     gene            complement(13594..14583)
                     /locus_tag="P5516_01290"
     CDS             complement(13594..14583)
                     /locus_tag="P5516_01290"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRT91102.1"
                     /translation="MTHPMDLISLQDIKDAHKRIQPYILRTPLIHSVFLSERVNAPVS
                     LKLECLQPTGSFKLRGAANRLLQLTEEEKTRGVITVSSGNHGKALAYMGELLGVPVTI
                     FLSTHTPACKVESLRKTHAHIIVTGNSYDEADAAAIEYEEKNGLVHVSSFDDLRVIAG
                     QGTMALEILADAPDTCHIIYPLSGGGMGAGIGLVSRQQSSPVQTWGVSMERAPVMVRS
                     LEAGRVLELPEEATLADGLKGGLGTPNLYSFAICREVVNHTALVSEEEIGQAVAYLFY
                     KHRLVVEGSGAVGVAALLAGKIKPDAPTTVVISGSNINLPTFMELVERYSALF"
     regulatory      14981..15089
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            15116..15772
                     /locus_tag="P5516_01295"
     CDS             15116..15772
                     /locus_tag="P5516_01295"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HRT91103.1"
                     /translation="MSINHVLVFANGELMDPRAAREMATRADWIIAADGGLAHVQALN
                     LKPDLLVGDLDSVSPEQIRWAEKMGAEVRRFPLDKDETDLELALQEAVATGCSRITIT
                     GALGGRLDQTLSNIYLLNLPVLADLDVRIDDGAQEVILIHSAVALQGSPGDTVSLLPL
                     SPIVRGISTSGLKYPLTDESLIFYRSRGISNEMSAENANVQIQSGILLCIHARREVTL
                     "
     gene            15769..16842
                     /locus_tag="P5516_01300"
     CDS             15769..16842
                     /locus_tag="P5516_01300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HRT91104.1"
                     /translation="MKRRLWVCALLGALLMTAGCARAVATETAPPVLTVMTHDSFSVS
                     ETVVKTFEEENGAKLVFLKSGDTGAALNRAILTRSHPEADVFYGIDNTYLSRALQEQI
                     FVPYTPAALAQLPGEFKSGLSGMVTPIDFGDVCINYDKAWFIERGLALPATLADLAKP
                     AYKGEAGNGLLVVENPATSSPGLAFLLVTIAEYGETGYLDYWRSLKQNGVVVVNDWET
                     AYYTNFSASSGRGLQPMVVSYATSPAAEVIYASEPLNEAPTASITTANTCFRQIEYAG
                     ILKGTSQLELAQRFIDFMLSPVFQEDLPLQMFVFPVNPAATLPDDFLQYAAFAQEPAR
                     IDPVLISKNRDKWVTEWTRVMLP"
     gene            16857..18533
                     /locus_tag="P5516_01305"
     CDS             16857..18533
                     /locus_tag="P5516_01305"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRT91105.1"
                     /translation="MTRSVKQSGVLLWGAPLALLAVFFFIPMAAILNVMFAQSGDIVF
                     SDSLRPLGFTVFQALLSTLLTLLLGLPAAYVFARFDFTGKRGLRMLTMLPFVLPTVVT
                     AAAFNALLGPRGWVNLLMMKLTGAQAPPINFLNTFTAILVAHVFYNIAIIIRVVGNAW
                     SQLDPRLENSARVLGASPWRAFIEVVYPLLRRPILSAALLVFLFDFTSFGVILMLGGA
                     GFSTLEVAIYTQALHNLNLPMAGLLSLIQLACTVIILLLYGWSSQGNDVPLFPRLSGE
                     GTRKPRSAVEKGTVALMVIILFTLQVLPLFALVSRSLVRLEGERGQRGKVQSGLTLDY
                     YHELFVNRQQSVFYVPPSRAVINSLSYAGITMLLSVGAGTLLAYAMVKRPATRRWLDP
                     LVMLPFGASSVTLGLGFLIAFSGSMLSRASFPWLVPLAHSLIAIPFVLRTVQPALSVM
                     PKSLCQSAAVLGASPARAWWEVEMRLLSRPLLVGGLFAFTISIGEFGATTFIARPDNP
                     TVPVAIFRYLAQPGGLNYGQAMAMATILIVICAVSIGLLESLSRAINGSE"
     gene            18544..19599
                     /locus_tag="P5516_01310"
     CDS             18544..19599
                     /locus_tag="P5516_01310"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91106.1"
                     /translation="MDRLEIIDLHKMYEGAPLLDGVSFYVVAGETLCLLGSSGSGKST
                     ILRIIAGIETQDAGSILWRGKDVSAVPAHRRGFGLMFQDYALFPHLNVSENIAFGLRM
                     QHAAKDQLGARVFEALERVNMTAFASRRVTDLSGGEQQRVALARALAPRPQLLMLDEP
                     LAALDRSLRVELQEDLRELLHQTGIPAIYVTHDQEEAILLGDRLALLHNGRIVQSGLT
                     EEVYRQPANTWVARFLGMTNLLEGRVVSVNPLMVQTACGTLGPFVPGYPSLLSPGQVV
                     HLLVRLDGLFSGEHQPSAGSINGEVTECRFMGDHYRVRMKVGECASLECFTASPLVLG
                     KALELRLNAEDVVVLPE"
     gene            complement(19639..19806)
                     /locus_tag="P5516_01315"
     CDS             complement(19639..19806)
                     /locus_tag="P5516_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91107.1"
                     /translation="MTQPEKELPDVKNQELLASRKRQVWQVVLVMTLSFCIGIVLFML
                     ILWWVFQKISP"
     gene            complement(19803..20378)
                     /locus_tag="P5516_01320"
     CDS             complement(19803..20378)
                     /locus_tag="P5516_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024937.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HRT91108.1"
                     /translation="MDIFNIFTAFGLSASAGLNAYIPLLVVSLLAKFTNLIKLQEPWN
                     LMTSWWVIGVLIVLTIVEILADLFPGVNHVNDVIQSFIRPTAGAVVFAATSSQVGDVN
                     PILGLIAGLLVSGGVHAVKALAVRPAVNAATGGLGNVPASIGEQATSTVVSILSVIIP
                     VIVAITLTIVLIIIILWANNRKKKRETQPVE"
     gene            complement(20396..21190)
                     /locus_tag="P5516_01325"
     CDS             complement(20396..21190)
                     /locus_tag="P5516_01325"
                     /inference="COORDINATES: protein motif:HMM:NF021384.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HRT91109.1"
                     /translation="MSEKKDDTPQKFRWLNPALVLLLVALFSAWLLLTPPGIEGKMNA
                     IGFSVCHQIDSHSFSVGGKVLPLCARCTGTFLGLFISMVYLYSKDRKSGFPSRQKITV
                     LLFFFAFFAFDGINASLPLVGLKTVYTPSNQLRLISGLLFGITLANVLLPLWHQTLWS
                     NPNPQPVWHSWQQLGWLFLFCALAGVMVVSDVPILFYPIAILSTLTIYLVLGMIYTLL
                     WCIILKKENSLHLFRDGFRILSAGLITAITQVGLMDLIRFSLSQTW"
     gene            21634..22863
                     /gene="pepT"
                     /locus_tag="P5516_01330"
     CDS             21634..22863
                     /gene="pepT"
                     /locus_tag="P5516_01330"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002448057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045148 - tripeptide aminopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HRT91110.1"
                     /translation="MPTVKERFIKYARINTESNRDSETCPSTQAQFDLARVLESELRE
                     MGLQEISLDENCYLMASLPANTEKPLPVIGFIAHLDTSPDMSGAQVNPQIVASYDGGD
                     IVLNHELGIQMKPMDFPELQKYIGQELITTDGTTLLGADDKAGVAAIMSAMEHLVAHP
                     EIKHGKIRVGFTPDEEIGRGADKFDVEKFGASYAYTVDGGEIGELEYETFNAAEVQVA
                     VSGRNVHPGVAKNKMVNSILLAMEFNAMLPVNERPEFTEGREGFIHLLGVSGSVENTR
                     MYYIIRDHDRMLLEKRKQYMIDAATLLNRRYGEGRIGLEINDQYYNMREKIEPVFEIV
                     ENARDAMLALGIEPKILPVRGGTDGSRLSFMGLPTPNIFTGGLFAHGKYEVLPTRSLE
                     RAVEVIIKIIEMAGKDK"
     gene            complement(22889..23758)
                     /locus_tag="P5516_01335"
     CDS             complement(22889..23758)
                     /locus_tag="P5516_01335"
                     /inference="COORDINATES: protein motif:HMM:NF015132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HRT91111.1"
                     /translation="MFTLLVVSVIWAFSFGLIKGNLTGVDSNFVAFARLFIAALVFLP
                     FIKWKKIQTKTAIKLALTGLVQFGMMYIAYLAAFRTLKAYEVALFTIFTPIFVTLIND
                     AFMKHINLLHLAVALITVAGTAIIEWESIQTPAVWNGFLLVQLSNLCYAFGQIYYRRL
                     MAQHPELKDQDVFGYLYLGATVITAAATLFTTPFNTLELHSKQSLTLLYLGVVASGVA
                     FFLWNIGARRVNAGALAVFNDLKIPLAVAVSLLVFGESANLPRLLSGGGIVIGALVIN
                     EWLSKRNLVKITG"
     gene            complement(23999..24682)
                     /locus_tag="P5516_01340"
     CDS             complement(23999..24682)
                     /locus_tag="P5516_01340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91112.1"
                     /translation="MKPESGISKTLRFIGILFMALTAAVTVLAGVGTSCVAFLPNNWG
                     PSMAKIAPMQWLYILYVLLTIAIGVLGIRAVVLLAKGRQGAYKAALIALVAGLVVGVV
                     HMITSRMLRGSSMPVDGIVYITVLTLVIFLVFRIPAIWNGVDYSKAPRKEGKRSSGAA
                     AIVAGALCLTIQFFMAPTHTWGGVNYADAFNTSMTIVGSGLVLLGIGLILSSVLVLAP
                     MKRKVDTLR"
     gene            complement(24706..25557)
                     /gene="cyoE"
                     /locus_tag="P5516_01345"
     CDS             complement(24706..25557)
                     /gene="cyoE"
                     /locus_tag="P5516_01345"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:TIGR01473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HRT91113.1"
                     /translation="MKLLHKFNHYWSLIKSLQTALLTVTGIAGYMSARCPFTHWTTMT
                     GLIASLLLSISGSTVLNMWYDRDIDSVMNRTHHRPLASGTISPSEALWLGLGLSAAGV
                     ILAAALSPLYGLLIFAGIFFDVIVYTILLKRRTCWSVVWGGIAGGMPILAGRALGMGQ
                     VDLIGILLALAILFWIPTHILTFSMRYQQDYDKARIPTFPSTYGEKVTRRIVALASLV
                     AAGLIGTAAVMVGVKQGALQVLGILSGGLLVMAVLMLFKPNSRLNFHLFKYASVYMLA
                     SMVLLAF"
     gene            complement(25564..26469)
                     /locus_tag="P5516_01350"
     CDS             complement(25564..26469)
                     /locus_tag="P5516_01350"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HRT91114.1"
                     /translation="MKKSIITMSLFLLFALTACQPVDFQAAPPVFDTGMDSEQWVEIP
                     AGEFLQGQFKKETTIDHAYEIMVTPVTNAQYAAYLNEALAADYIQVNGNQVEGYYPGD
                     AFAGGRHEEEIKAGSYIHIPLDDPFLRLSYDGNSFTVKPGWENHPMTMVSWFGAKSYC
                     EFNGWRLPTELEWEKAARGTDGRAYPWGEEIARNNANFYFSRDPFENMATFGSRTTPV
                     GFYNGKTYNHYTTLDSASPYGVYDMAGNVWQWTANVYDAQHYRYMRGGSKDMYENNLR
                     VWSMNNATPTFTSPGTGFRCVRDVE"
     gene            complement(26469..27950)
                     /locus_tag="P5516_01355"
     CDS             complement(26469..27950)
                     /locus_tag="P5516_01355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91115.1"
                     /translation="MQWLNKRYQFLFRSTKGLILVAIAFAALGSAFFGLLSGPMADYG
                     IKDVVVRALNINLEPAEREGRLVILYHSLAMIAIAVEVYMITDLLKMKDNHRLPINTT
                     VTVGYLMVMFGGLPFAYWGHNWVLHGIYIAGLSLIFFAGCLLLVALWPWRDEYRLKDK
                     DYAQTKKGYDMERIAFFMVSLTTLISAIFGAVPGSFYGNGFETFLAENVVRDPHKTAL
                     DLSIIGHLHIMLALIAIMCALIIGRWMDFKGMWQKLAMPLYIVGAFVLSLGTWLVVQF
                     EEIAHFIIYGGSTVAMLGALFLVIDSWKKIIRSGLDEKGIRKGNFFQGVAALLRDPVR
                     FGPTWQMVFMNFTVSGVGIFTAVKLDEIFRKWQFMDERSILTGHWHILATLTATILLL
                     YYADLAGLKGKARKWFGWAVIIFSDVAFAAVTVYEMKRLFITEGEQQPLVNRAVLAGD
                     IGVIVLLLALAALMVWRLTDLFKKKGRWQEETTHRLAKKEEVQ"
     gene            complement(28062..28748)
                     /locus_tag="P5516_01360"
     CDS             complement(28062..28748)
                     /locus_tag="P5516_01360"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRT91116.1"
                     /translation="MSETRELAERLKKITHFSEIPLSGVEQIIASGEIRQYVKGAFVF
                     HEGDESAGMFVLLNGQVNLSKLGPEGKEGILNTLEPVIMFNEVSALDGGPNPVSAYAH
                     TDIRLWRIGYLPFQQLLRQYPQIGISLLGVLARRNRLLLTHYDDLSFRTVQARMAKHL
                     ISLSENGRRSINRMENPIHTIAARIITSPEAVSRTLKVFKSAGVLRANRTEIEILDPR
                     HLQELAKIDL"
     gene            28805..30205
                     /locus_tag="P5516_01365"
     CDS             28805..30205
                     /locus_tag="P5516_01365"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HRT91117.1"
                     /translation="MTSFTFEKFIASYPGYTDTSYLDRLRELDYKRLEREKQVYLDYT
                     GGGLYADSQVIKHQRMLLEGVYGNPHSSNPTSSTSTALVEDTRARVLRFFNASPEDYV
                     VIFTANASGALKLVGEAYPFGQDCCYLLTFDNHNSVNGIREYALAKGAHVRYLPVLPP
                     RLRAVEGKLDEYLANARGSCHKLFAFPAQSNFSSVQHPLEWIDKAHAAGWDVLLDAAA
                     FAPTNRLDLSRWQADFVPISFYKMFGYPTGVGALIARRAALKKLRRPWFAGGTITVAS
                     VQGEKHYLADAPAAFEDGTLDYLNIPAVKIGLEHLESIGMELIHERVKCLTGWLLTCM
                     KDLRHRNGKPLVKIYGPSDTKQRGGAVTVNFFNPEGMVIDHRWIEEKANARNISLRTG
                     CFCNPGAGELALHISSEELSSCFNRPDHEQRLTYDEFRLCLNTKASGAVRISVGLVSN
                     FADGQRLLDFLATLVE"
     gene            30202..30771
                     /locus_tag="P5516_01370"
     CDS             30202..30771
                     /locus_tag="P5516_01370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91118.1"
                     /translation="MTDRMAALSHFLYRFSRGWVVLLGFIVFIGFSTLTLPGQNAIVD
                     EYSQGSGSPDTSLFYNAKQLYHMAEVYGQEGRTAYLNARWTFDVAFPFIYTFFLVSAI
                     GWLLNQSLPVGSRWRLINLVPLAALGLDFLENTATSVVMARYPHHCSLAEWLAPVFTL
                     LKWLLVGGSLVVLLVAIFLVLLRRIKKNA"
     gene            complement(30828..31526)
                     /locus_tag="P5516_01375"
     CDS             complement(30828..31526)
                     /locus_tag="P5516_01375"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinone/menaquinone biosynthesis
                     methyltransferase"
                     /protein_id="HRT91119.1"
                     /translation="MAHLLGTERERYVKKTFKSVARRYNMMNHLMTFGVDILLRKEVM
                     RIAKLRNGETLLDIGAGTGDLSREARHTCPGAGINAVDFSLEMMAAGKDWKNIHRCQA
                     DALALPFSHNTFDVVVSGYLVRNVVDLDAALAEQYRVLKSGGRLVVLDTTRPRWKPLN
                     PLIKFYFRYVIPNLGRLFTGNSEAYTYLTQSSENFISAEDLADSLRRCGFTGVNFRVR
                     MLGTMAIHSASKRP"
     gene            complement(31766..31838)
                     /locus_tag="P5516_01380"
     tRNA            complement(31766..31838)
                     /locus_tag="P5516_01380"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(31802..31804),aa:Glu,seq:ctc)
     gene            32041..33135
                     /locus_tag="P5516_01385"
     CDS             32041..33135
                     /locus_tag="P5516_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HRT91120.1"
                     /translation="MSVDFIFRILGMVILAIVGGILGNQFGEIANTSPSATTFPVEQY
                     RFLFGLLGALFGLILTPYFTTRPARAMRNALAKATTHALVASLFGLVAGLIVAALLAF
                     PLSLLPDPFGSVLPFVGAILFTYLGVVIFKLRQQDFLNLLSNLRSGSGVTAKDGASGA
                     GWAESRTILLDTSVLIDGRIADISRTGFLPGSLLIPRFVLNELQYIADSPDNMRRQRG
                     RRGMEVLSALQKEPAIPVRISDIDVEGMREVDDKLVVLARQMRCPILTNDYNLNRIAE
                     LQGVTVLNINELANAVKSVLIPGEIMTVQVIQEGRETGQGVAYMDDGTMVVVENGREY
                     MDKSIQVTVTKVLQTAAGRMIFARPDEAAN"
     gene            33155..34549
                     /gene="cysS"
                     /locus_tag="P5516_01390"
     CDS             33155..34549
                     /gene="cysS"
                     /locus_tag="P5516_01390"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HRT91121.1"
                     /translation="MKVYNTLSRKVEEFQTLEPGKVKMYVCGPTVYAKAHIGHAMSAL
                     VFDIIRRYLEYRGYEVRHVMNFTDVDDKIILRAQEKGVDPFKLGEMYIHEFEKHLQDF
                     NILPATVNPRATREMEQIITMIQGLVEKGFAYDKDGDVYFRVTSDSQYGKLSGRRIDD
                     MQSGYRIEVDERKEHPMDFALWKAAKPGEPAWDSPWGKGRPGWHIECSAMNLNHLGEQ
                     IDIHGGGNDLVFPHHENEIAQSESYTGKTFARYWIHNGMLQLSGEKMSKSVGNLVTIE
                     EFLENHPADALRMLVLNSGYRNPLSFSDEILAQAEKAIERLRSGLKPAPAAAPGAAQS
                     VVDALARQVETAESGFVQSMDDDFNSAGALAQLFDLVRGINQARADGATHAQLSAAQL
                     KLRQLTAVLGLSLEKETETHQEVDGFVNLLIEIRRELRANKLWELSDSVRDRLAALGV
                     VLEDSKEGTSWHWK"
     gene            34557..35294
                     /gene="rlmB"
                     /locus_tag="P5516_01395"
     CDS             34557..35294
                     /gene="rlmB"
                     /locus_tag="P5516_01395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HRT91122.1"
                     /translation="MKEWITGRNPVMEVMQAKRRQVFRLLLASGVEEKGRIAEILRAA
                     ASIKAPVERVARERLERLGENPQGVAAEVGVYPYVELYDIYDLAKERNEALFVLVLDV
                     IQNPQNLGTLLRTAEAVGVHGVIIPQHRAAEVTPAVVSASAGASEHLLITRSNIARSL
                     EQLKEAGAWVVGLAEGEDAPAEVPLGGPIALVVGNEGEGMRALVRQQCDYILSLPMRG
                     RIQSLNAAVAGSIVLYQVLLARQKTSR"
     gene            complement(35583..36083)
                     /locus_tag="P5516_01400"
     CDS             complement(35583..36083)
                     /locus_tag="P5516_01400"
                     /inference="COORDINATES: protein motif:HMM:NF016213.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 domain-containing protein"
                     /protein_id="HRT91123.1"
                     /translation="MDAIANSNWFVPVLAVVMGVGLFGLMLYIIPGLTIIWLAILAYG
                     IVSGFSPATGILFAVITLLMIGGNLIDNLFMGAEAKKTGASWISVVVALAAGIAGTFI
                     LPPFGGLLFAALSILVVEWVRKRDLKEGAKSAGAIIRGCGFATIARLGIGVLMIGLWV
                     VWVLWL"
     gene            complement(36086..37354)
                     /gene="serS"
                     /locus_tag="P5516_01405"
     CDS             complement(36086..37354)
                     /gene="serS"
                     /locus_tag="P5516_01405"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HRT91124.1"
                     /translation="MLDINFIREHPEELRKALTDRQMDSSVVDKLLTMDAERRGLLVK
                     VENLKALRNTVSKEISQTKDPAERQEKIDAMRSVGEEIAQLDETTRQVDEGLQALLAT
                     IPNLPDPSTPIGKDESENIVLRTHGALPKFDFTPLPHWELGPKMGLIDFDRGVKLTGS
                     RFYVLNGEAARLQRALISWMLDLHARQGYVERYTPFMVKAETLYAAGQLPKFVDNLYH
                     DIEEDLWMVPTAEVPLTGMYRDEVLEEASLPLRFTAYTPCFRREKMSAGRDVRGIKRG
                     HQFDKVEMYIYSTPEESMHELQKMLADAEETCALLGLPYRVKQLCTGDLGFNSVVTYD
                     LEVWAAGCNEWLEVSSVSNVGDFQARRANIKYKPAEGGRNRLVHTLNGSGLGLPRTLI
                     AVFENNQQADGKIKVPEVLLPWMGGLKVIS"
     gene            37750..38361
                     /locus_tag="P5516_01410"
     CDS             37750..38361
                     /locus_tag="P5516_01410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRT91125.1"
                     /translation="MDEQALVADALQGNVDAFNRLVLAYQDMAFNLAFRMLNDDDLAA
                     DAVQNAFLSAYRNLRAYRGGSFKAWVMRMVTNGCYDELRRQKRRPTIPLEPQNPEDGE
                     EIESADWLASDDPQPEQAFDQAELEHALQHCLENLPPDFRAVVVLTDVQGMDYEEVSR
                     AVRTPLGTVKSRLARARQRLRDCLRQFQELLPTQFRLTGGERR"
     gene            38358..39215
                     /locus_tag="P5516_01415"
     CDS             38358..39215
                     /locus_tag="P5516_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91126.1"
                     /translation="MTERLTSRDWILISAYLDGRLSPAALTRIEKRIQEDAHFKRSLE
                     EIARLKQLLAALPKKRAPRNFTLAPEKAQKPLRGLWLQPALSFVSVTASILLVLVFSF
                     NYLGMGLPRQAAAQRAEIAAMEGVAEAPAPMIINWIPSGGQGGGAAEAKSPYTGIDGI
                     GGPGFPVAEPGIGGGPVEPAVPELPPGVDPQPVMNEEPEPPAYDEPAPAALEGEPVSD
                     ADLSNLILGLAEDDTAGQVIESAPLRKAPAPQPRRGFSAPSLVMLIAGITALLSGAGA
                     LFLRKKFHA"
     gene            39208..39720
                     /locus_tag="P5516_01420"
     CDS             39208..39720
                     /locus_tag="P5516_01420"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRT91127.1"
                     /translation="MHSHSHEHFKFCPQCATPLRMMMQLGERRPSCPACGWVHYEDPK
                     VAAGVLLLRGREVLLVRRVMEPYLGTWSIPAGFVNAFEDPAAAALRECREETGLTVEI
                     EGLFDLLTGREHSRGADIFIVYRAHFLSGSLAAADDADQVGWFPLFDLPPLAFETTRR
                     VLEKARHLQL"
     gene            39958..42303
                     /locus_tag="P5516_01425"
     CDS             39958..42303
                     /locus_tag="P5516_01425"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HRT91128.1"
                     /translation="MAKSTKTRKSYAHPPKSSRSSSRRGQYSRSTLTLDQRLNFIGGV
                     TVLTGVLSIVALFSNANGPLSGWIAQTTTRLAGWGGVIIPLALIIGGTVLLLRHYENL
                     PRVSYWRLAGIILLYLNILGWFHFIAGGGFSTAKDGRGGGYIGALFDRVLANSLGPAG
                     EGVFLFAWLLVAILFILNLPLVEIAARVNTLLVRFKKPKEENPRQPMFHFTSARPYRV
                     KNESRTDLPDGYKPLVVEEESPATVTVIGSPSAAEPKTTGVREKPARVAPEPSESLPV
                     TRTRVTTPVAPWPIPAVSDILDLATPAVVQSHYDQGRARIIEETLASFDAPGRIVEIQ
                     RGPAVTQYGVEPEFIESRGGRTRVRVSKIAGLANDLALALSAPSIRIQAPVPGKAYIG
                     IEVPNSELSLVSLRELIETDAYKKIRSSLRFAFGKDVAGKPICADLTSMPHLLIAGTT
                     NSGKSVLVNAILTSLLLNNSPLDLRLVLVDPKRVELTGYNGIPHLLAPVVVDAERVAG
                     ALQWVLGEMDSRYRRFAEEGVRNIMDHNRKEAERLPYIVVVIDELADLMLLAKDETER
                     SITRLAQLARATGIHLIISTQRPSVDVITGVIKANLPARVAFMVASNLDSRVIIDKPG
                     AETLLGRGDMLYQAPDAPAPVRLQGVYVSDQEIQRLVNAWRLIVANRRAEGGKELFTA
                     TFEYQPQHVPFKQPSFFGDGAQEDDPVLKNAIEIVRKEGKASTSFLQRRLGIGYTRAA
                     RLIDTLEEQKIIGPRQMGSQVREVLDFGADNEEPPAETQSD"
     gene            42496..43455
                     /locus_tag="P5516_01430"
     CDS             42496..43455
                     /locus_tag="P5516_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91129.1"
                     /translation="MRKRNSFFLLIAVLLLGFLASCRSARLPLPAFLSADTPTPTATM
                     TFTPTYTPLPTKTFTPTPTPPVNLIPCANSDACSEGVYIREYLETVGLLDEADEGERP
                     FEVEVPYNLPLRFGLSWIAIDDETLADNMKKMTFFFEIDGQPYLDKNDLIYEYYPDVE
                     DPSIVYSSVFIDYLLTGWRIGEPHMVSIGFRFDQSLSDGWNTYYLGDTIGYSYRILPV
                     ELPTATPTPSPTSTPQPRPTAVPPTPTVACELGTTIEIKNDTGGQVTMHFTGPVKYTF
                     YIAPGSRTLQLCTGQYSYTAYGCGGSSTSGSAGDGDEIEFWCE"
     gene            complement(43503..43913)
                     /locus_tag="P5516_01435"
     CDS             complement(43503..43913)
                     /locus_tag="P5516_01435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91130.1"
                     /translation="MNNHPLQPPNVQAPRSSLLKLVIISAFIAMVAFCAPAGVLAVIF
                     TRPLFALTTGVVCPPGSEMQFEEWEEEGDWEEEASTQFRAYCVDSSGQQVQERTLQAL
                     AVLMCSFFLAGFFIVLAVLLIVRAVRHRNRSTNP"
     gene            complement(44118..45332)
                     /gene="metK"
                     /locus_tag="P5516_01440"
     CDS             complement(44118..45332)
                     /gene="metK"
                     /locus_tag="P5516_01440"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017370549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HRT91131.1"
                     /translation="MSGSSFMQNPSFKFTSESVTEGHPDKLCDQVSDAVLDACLEQDP
                     RSRVACETAVKTGYVMILGEITTNAFVNFNDLVRQVVNSIGYDSSDKGFDGNTCGVLS
                     AISSQSQDIALGVNKSYEAKGGEDKETELEHIGAGDQGMMFGYACDETPTLMPMPIYL
                     AHRLTQRLAEVRKAGLLPWLRPDGKSQVTVEYAYGKPVRVDTVVVSTQHTADVSHADI
                     TEGVIEHVINPAIPAELLDGTPKIYVNPTGRFVIGGPMGDAGVTGRKIIVDSYGGMGR
                     HGGGAFSGKDPTKVDRSAAYAARWAAKNIVAAGLATRCEIQLAYAIGVARPLSINVET
                     FGTGVIPDEKIAALVSEHFDLRPGAIIRDLDLRRPIYRKTSNYGHFGRELPEFTWERT
                     DKAGLLRSAAGL"
     regulatory      complement(45408..45516)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            complement(45635..46594)
                     /locus_tag="P5516_01445"
     CDS             complement(45635..46594)
                     /locus_tag="P5516_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91132.1"
                     /translation="MSSSSSSSAKTAHDLYTCPHLGMLDDTTTTTAFPSEVNHCLHCQ
                     SVCVPSRQQQQEYCLSQRYRDCPILHQAAPARLPASLRWVQNPAVFRAIFIKSALAVF
                     GAGLLAGFFLLGAPARISHIIQAYVPEDTQTPYQPYDFPTRTPSPRPEQPSPTATSLL
                     SHLEIPTALPISQIVQLTVNEVELGVNCRSGPDSSFESYLILRTGDVLQALARSPDDA
                     FFYVRLPKKRAPDCWVPAAFVNVLGDTHTLPVLTPAPTPHPTVTLTPLPSTPAETPQT
                     PPTARPTSTTQVNTPPPSQPTAESPEPTEEPPEPTEEPSATPG"
     gene            complement(46820..47740)
                     /locus_tag="P5516_01450"
     CDS             complement(46820..47740)
                     /locus_tag="P5516_01450"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016891 - endoribonuclease activity,
                     producing 5'-phosphomonoesters [Evidence IEA];
                     GO_process: GO:0042779 - tRNA 3'-trailer cleavage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HRT91133.1"
                     /translation="MFEIIFLGTSASAPSPRRGLPAYLVQHDEYRFLIDCGEGTQRQI
                     LQAGIGFKRLNRILITHGHLDHILGLGGLLSTFMRWETIDRLEIYGGANALERISDLV
                     HGVVLRGARAPMPVELIPVTPGVIFEAPSFTISAFAVNHRGPDNYGYLFEEKGRQPFL
                     AEAADALGVPAGPLRGKLVKGESVTLLDGRVITPSMVTGPFKPGARLAALGDLGETAS
                     LTEVLRSVDGLVMEATYLEEEASMARQYAHLTARQAGEFAASIGAGKLILTHLSRRYR
                     DKDILAEAQAVFENTSVARDFDSYTIRKEA"
     gene            47897..48385
                     /locus_tag="P5516_01455"
     CDS             47897..48385
                     /locus_tag="P5516_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine-specific kinase"
                     /protein_id="HRT91134.1"
                     /translation="MEFLSVSIHKPEALNFILGQSHFIKTVEDIHEALVTAVPGIQFG
                     LAFCEASGACLIRSSGTNEALTQLARENAMNIGAGHCFILMLGEGYFPINVLNLVKNI
                     PEVCAIFCATANPTEVVIYDNGYGRGIMGIVEGEKPKGFESPEDVRWRVDFLRRIGYK
                     AG"
     gene            48421..49368
                     /locus_tag="P5516_01460"
     CDS             48421..49368
                     /locus_tag="P5516_01460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91135.1"
                     /translation="MKKLIPVLIILSLVLTACGNLPLGRTEEDMSDSEMATRVAELLS
                     TMTTPTTEMVFPPTPTPQAATEAPPEPSAAPATQEPAALPATETPVVVEETLLPEEAG
                     TPTTEVTPALPETDPARKLGNPTHSDPLDSYEKWTWPTGSDDYLKVNFKDGFLEMTGL
                     TTMAGWRLPMLAQQTNSYIELTVNSGACQGKDSYGIIFRVPVFKEPTQGYLYEVTCDG
                     YLRLWEWNGKAKPKGEADVLINWKESMDIKTGANQVNRLGVMAVGKSLRIYMNGIPQG
                     EVSDSTYPAGFVGAFVRSVTTPKYTVKFDEMKVWENPAQ"
     gene            49498..49917
                     /locus_tag="P5516_01465"
     CDS             49498..49917
                     /locus_tag="P5516_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR00149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HRT91136.1"
                     /translation="MKSYRKEITLNIPARRGFVNITPQVQACLRESGISEGLVLVNPM
                     HITASVFINDDESGLHRDYDAWLERLAPHEPVNQYRHNDTGEDNADAHMKRQLMGREV
                     VVAVTGGRLDFGTWEQIFYGEFDGRRPKRVLVKIIGE"
     gene            49919..50776
                     /locus_tag="P5516_01470"
     CDS             49919..50776
                     /locus_tag="P5516_01470"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRT91137.1"
                     /translation="MSNRVYFGENLEVLRALPDASVELIYIDPPFNTGKVQARTRLRT
                     TRAENGDRVGFQGQRYQTVRLGSLGYNDIFDDFLAFIEPRLLEAHRVLSAAGSLYFHI
                     DYREAHYCKVLLDSIFGRACFLNEIIWAYDYGARTRRKWPPKHDTIFWYVKDPLRYTF
                     NYDAIERIPYMAPGLVGPEKAARGKIPTDTWWHTIVPTNSGERTGYPTQKPLGILRRI
                     VAASSNPGETVLDFFAGSGTTGAACLELGRRFVLVDNNPQALAVMTERFKGVTDIEWI
                     IDTPEAMDR"
     gene            complement(50953..51873)
                     /gene="lipA"
                     /locus_tag="P5516_01475"
     CDS             complement(50953..51873)
                     /gene="lipA"
                     /locus_tag="P5516_01475"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HRT91138.1"
                     /translation="MANTPTRDRIQPAPSDPRPRRRPDWIKVKAPTGETYAQLQALMR
                     RTSLHTVCEEAGCPNMGECWGSGTATFLMLGEICTRSCRFCDVKHGRPAPLDWEEPER
                     VAQAVKAMNLKHAVITSVNRDEREDGGAPIFAAVIRRIHELVPGCSVEVLIPDFKASL
                     PALKMVMDARPEILNHNVETVPSLFKKVQPQDHYEWSEAVLRHAKLLAPDVLTKSGLM
                     VGLGETMSELKAVMRDLRDWGVDILTLGQYLQPSRHHLPIERYYTLEEFADLKEYGLS
                     LGFGWVESGPLVRSSYHAAEQVRALNPTFR"
     gene            51997..52665
                     /gene="rsmI"
                     /locus_tag="P5516_01480"
     CDS             51997..52665
                     /gene="rsmI"
                     /locus_tag="P5516_01480"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HRT91139.1"
                     /translation="MLILVATPIGNLGDISQRALAALENADVVVSEDTRKTGFLLNHF
                     GIQKPQISFREDNEQRALPKVMEFLELGKTVALVTDAGTPSISDPGFILVRSAIEKAI
                     EVSAVPGPTALIMALVLSGLPVHSFTFRGFPPRKSAARQRWLEIDCDSPHTLIFYESP
                     HRLLAFLQDAQAVYGNRRCAVANDLTKKFETVYRGTLKEVSEQLAREKLLGEYVVVIA
                     GAED"
     gene            complement(52662..53387)
                     /locus_tag="P5516_01485"
     CDS             complement(52662..53387)
                     /locus_tag="P5516_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HRT91140.1"
                     /translation="MDKTPQRSYRYFDLVMALFVTVLIISNIASSAKIVDWGVSIFNL
                     PLAFDAGTILFPISYIFGDVLTEVYGFKRSRRVIWTGFGCLALTAAVFWLVRVMPGEA
                     TWVEQSGQGAYNAILGGLTSGGIVLASLLAYLAGEFMNSIVLAKMKVATRGKWLWART
                     IGSTLVGEAVDSLVFVSIATLTGVFPWSLFWTLTVTNYLFKTAIEVLMTPVTYWVVST
                     LKRQEQEDYFDTHTRFSIFDWNG"
     gene            53460..54233
                     /locus_tag="P5516_01490"
     CDS             53460..54233
                     /locus_tag="P5516_01490"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmE family RNA methyltransferase"
                     /protein_id="HRT91141.1"
                     /translation="MQRFFVNPEEIQGQEVVFAPARSRQIARVLRLKNGQNCMVLDNR
                     GSELLVELTQVEAGACRAVIVERAEAQRERIDLLMLLSLTQREKFEWMLQKCTEVGVS
                     TFQPVITSRSLVQQPAQTIAKYERWKTIIWEAAEQSARSRLPELLPPAGLAEAVAEAA
                     RRCQRCLIPWEGEKARTLKGALAGPAVGSVALFIGPEGGFAEGEVQQAVNASFNVVTL
                     GRRILRMETAAVVSAALVLHELESTEADDGGTGSSGPIG"
     gene            complement(54184..55680)
                     /locus_tag="P5516_01495"
     CDS             complement(54184..55680)
                     /locus_tag="P5516_01495"
                     /inference="COORDINATES: protein motif:HMM:NF013362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91142.1"
                     /translation="MSQKPNRRQRPTVPPADSGEMAAAYKPLLTEEEFKLLVNEINQP
                     LPTAIRLNPLKAPPNLASRLSEKYGWQLEAVPFCPAGYRVTIGAGPEVSAVLEHRLGL
                     MYIQEAASMLPPELFNFSPQREELVLDLAASPGGKTTHLISRLEDSGLVIANDSSQGR
                     IQALRLVLQQWGAANAAVSRFPGERFGEWFPSMFDKVLLDAPCSMQGLRTAESHISRP
                     VTEKESRQLSRRQLALLTSALRTLRVGGELVYSTCTLLPQEDEMVVEELLRVFGSAVE
                     ILDAQTKLPAPAPAITSLDGAELHPALSKAIRLWPHRYHTAGFFACHLRKIEHTPMRP
                     TSPPSRPLAQVGFEELTAREQRAFCEEFHNTYAYDLLADISSHQRTLVRHFEEMHLLP
                     MLLLEKFPALPLQSAGMLLGQQTASGFLPSHQWASRFGARCQRNIVQLDEIESRLWLS
                     GADLLPRGSPAKETDRFRIILNQEGLSLGCGKVTQSALKNLFPRHLLL"
     gene            complement(55677..56510)
                     /locus_tag="P5516_01500"
     CDS             complement(55677..56510)
                     /locus_tag="P5516_01500"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91143.1"
                     /translation="MKKISASPTEDQYAALLARVSASRKYRALGIPDTTLRDLLEKTL
                     RACSDPRQVEKVFRQKLHNLLATYLGDPDYTMMRQVLAALPNQPHAAEVMEWCAQLLK
                     AHASTRERLPMLSLFYHEIFRFTGTPRVVLDLACGLNPFALPWMGLPRETAYYAYDLH
                     QPRVDLIDAFFQHIGQTGSAYHRDILVEPPEMEADVVFFFKEAHRFEQRERGVNRQFF
                     HRLRTRWLLVSLPTVSLNGQRSLLTSGRRLVAEACAQRGWQVSELLFDSEIVFCIRAD
                     A"
     gene            complement(56524..57336)
                     /locus_tag="P5516_01505"
     CDS             complement(56524..57336)
                     /locus_tag="P5516_01505"
                     /inference="COORDINATES: protein motif:HMM:NF018758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91144.1"
                     /translation="MAEPDTLLDALVEQVQRAEKYRHLSEVLVRSVLTAEMEKRHGPK
                     EAVKAARSKLHQVAAAYQGKPIPYAAWLKELAQLPQDLHHPDVLSYLMHALSAHASTR
                     ERIPIQAVFFQECLAPLGSVHSILDLACGLNPLVIPWLPLQPGFTYTACDIYTDMLDF
                     LNAFFARFKIDGHAVPCDITRELPPVKADLTLVLKTIPCLEQIDKDAGRRLLEMISSP
                     NILVSFPAHSLGGKSKGMVRNYERRFAELLAGKAWQVTRFEFPGELAFLIQR"
     gene            complement(57329..58435)
                     /locus_tag="P5516_01510"
     CDS             complement(57329..58435)
                     /locus_tag="P5516_01510"
                     /EC_number="2.4.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRT91145.1"
                     /translation="MRAANLELVSGKVHFPVFLPDATYGVVRTVDARDLAGIGIEALV
                     MNTFHLTQKPGSSTIQALGGLHQMTGWHRPIITDSGGFQAYSLIRQNARYGSINDKGI
                     LFYPEGSSRKFQLTPQKAIQLQMSYGADIVFCLDDCTHVDEDEAAQRLSVERTIAWAR
                     LSKAAYLRLAEQKEQTGQQRPLLFAVVQGGGFKEQRKRCADALLEIGFDGFGFGGWPL
                     DGENRLLTEIIAYTRQLIPAAFPMHALGIGHPQNLLATYHLGYDLFDSAMPTRDARHG
                     RLYTFTSAPSPGAPGLTGDWFAYQYVDNPRSIRSSQPISQWCDCPCCRDYPLGYLYHL
                     FKQGDPLFMRLCTLHNLRFMVQLTEHIREQAAHG"
     gene            complement(58517..59764)
                     /locus_tag="P5516_01515"
     CDS             complement(58517..59764)
                     /locus_tag="P5516_01515"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT91146.1"
                     /translation="MKRVNGWYLFVLNAYWIGLSFMWNSLHVTILPAVLLKYVPDTQK
                     NTWLGLLTFFGLIIAMLVQPVSGAVSDHWRSKLGRRRPLMWFGTAGDLFFLLLMGFIG
                     GLPALFIGYIGLQTTSNIAHGPAQGLLPDEVPAEKLGLASGIKTALDMAGIVLSSLLM
                     GFLISSSDPNPRLAVTVIIALLVVFGLITLVFTKESPSIGEGRNHLQLGRAIKETFNF
                     KVVGDKHYWQLILSRFLYLVGIYGFQSFAQYFIRDKFPEQDPIMFTQFVMGTFVVVLI
                     IFSLLAGTLSDRYGRKKLQILSSIIGGIGAILLIFAKTPAQLLIYGGFMGVGLGIFLS
                     VNWAIANDMVPKDNAAKYMGLTNIATAGSAAIARLEGPMTDALNNAAPGQWWGWTALF
                     AISALLMFLSAGALLKVPETRKA"
     gene            complement(59804..60439)
                     /locus_tag="P5516_01520"
     CDS             complement(59804..60439)
                     /locus_tag="P5516_01520"
                     /inference="COORDINATES: protein motif:HMM:NF016041.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sterol desaturase family protein"
                     /protein_id="HRT91147.1"
                     /translation="MAHLPTDHSDVPIRLFKSDFLEFFSHISPITVLVIWLPVALFFL
                     IWSIAHAPAGGFPVVIPLGFLVGLFLWTLAEYVLHRFLFHMPVKGEKAERIIFLFHGV
                     HHAQPQLKTRLVMPPVISIPLAAIFYALFYLFFAVILKAAQWVAPVFSAFIFGYLLYD
                     LTHYATHHFPMRGGYAKYIKRYHMQHHYKTPNARFGVSSPLWDKVFKTMPE"
     gene            complement(60589..62148)
                     /locus_tag="P5516_01525"
     CDS             complement(60589..62148)
                     /locus_tag="P5516_01525"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HRT91148.1"
                     /translation="MKDHLLAIDVGTQSTRAMVFDLRGNLLAISRVPIEPYYSPAPGL
                     AEQDPQVFWKALCEACNSLWQQPGVAKENIAGVALTTQRSTLINVDKEGKPLRPAIHW
                     LDQRRTSDLKPVGGLWGLLFKIAGMSETVAYLQAEAEGNWLQKHQPEIWSNTYKYLLL
                     SGYLTMRLTGRFADSVGCLVGYLPFDYKKQDWSGKNDWKWQAVPVNKEMLPELVKPAE
                     QIGVITAEAAAETGIPEGLLLIAAAADKACEVIGAGCLNPAIPCLSFGTTATINTTLD
                     RYVEVIPLIPPYPSAVPGSYSLEIQIYRGFWMVSWFKREFGLNEERLAAQRGVAAEEL
                     FDDLVNAVPPGSLGLTLQPYWSPGLKVPGPEAKGAVIGFGDVHTRAHFYRSILEGLAY
                     ALREGAERTSKRTHVPIEEVRVSGGGSQSKAAMQLTADVFGLPASRPHVFETSGLGAA
                     IDAAVGLKLHSDFSSAVKEMTHKGETFEPKQENYAIYNELYRRVYLRMYSHLKPLYDE
                     IRAITGYPPKD"
     gene            complement(62150..63892)
                     /locus_tag="P5516_01530"
     CDS             complement(62150..63892)
                     /locus_tag="P5516_01530"
                     /inference="COORDINATES: protein motif:HMM:NF014910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HRT91149.1"
                     /translation="MVKEKKHKPNWYEEPVPPGTYRSLLKWGDPAAFKHPNSGLVKLM
                     MSSFDLAPEDLDQPIDLALDRVELERPVLLEKSHIDAFTAICGVENISTDTYTRISRS
                     YGSGMIDALRLRKKIIQNIPELVISPRSHSEVQEIITYSNQHQIPVYVYGAGSSVTRG
                     VEAVKGGIALDMSTHMNRVVSFNETDQTITVETGMTGPQLEEILNHAPERLGAQRRYT
                     CGHFPQSFEFSSVGGWVVTRGAGQNSTYYGKIEDIVISQRYATPRGELKTCPHPRSAN
                     GPDFDQIMMGSEGAFGVLTEVTLRVFRLQPGNRHRFSYMFKNWDDALAACREIMQCEC
                     GNPSVFRLSDPEETDVAMKLYHIEGTPADTVLRLSGYLPMQKCLLLGMTEGEAGFSRN
                     VNRQVRRICRRHGAFNISFAPVTSNWEKSRFRDPYMREDLQDFGVLTDTLECAVTWSQ
                     MKEVHASVRGFIKSHPNTICMTHLSHAYPQGGNLYFIFIAKIATIKQYLELQYGILSA
                     IQQSGAAISHHHGVGKQTAPWLEGQIGSAQMDIIRALKTHFDPRNIMNPGGTLGLDMT
                     TEQKEQRWSKDLEG"
     gene            complement(63905..65494)
                     /locus_tag="P5516_01535"
     CDS             complement(63905..65494)
                     /locus_tag="P5516_01535"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HRT91150.1"
                     /translation="MWEKGWRQRAWQELDGKWDILIIGGGITGAGILRRAVQEGYRTL
                     LVESGDFSSGTSSRSSKLIHGGFRYLRNKQFRVTRESVREREWLLKEARHLVTPLGFL
                     MPCPDDRKLTSQFAVGVILYDLLAPKWHHSRLNSHDMLDRCPALDADQLHCGFLYYDA
                     GMDDACMVLRLIRESVSAGGCAINYARAAALLKETNGRVCGAVIKDTATGGFQEKEVL
                     ARVVINATGPWSDEVRAGVNAPARLRKLRGSHLVFSRQRLNLPYAVTLLHPRDRRAMF
                     AIPWEGVSLIGTTDLDHTLPLSQGEPYTTQEEIDYILEAANATFPGLQLDRADIISTF
                     SGLRPVVNTGQADPSKESRAHVVWEENGLVTITGGKYTTFRIMAEDALRAAMPALPHP
                     ANLSRPKSYFSPLPTRSSPQGMAAETWLYLLGRYGNETIQLFENAEEGENQPVGSLPN
                     LWSEVRWAARCGAVEHLDDLLLRRVRLGLLLPHGAQEEMARVREIVQPELGWDDARWQ
                     AEFTRYVELYKKAYSPSPAGF"
     gene            complement(65513..66217)
                     /gene="fadR"
                     /locus_tag="P5516_01540"
     CDS             complement(65513..66217)
                     /gene="fadR"
                     /locus_tag="P5516_01540"
                     /inference="COORDINATES: protein motif:HMM:NF003444.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000062 - fatty-acyl-CoA binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0019217 - regulation of fatty acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid metabolism transcriptional regulator
                     FadR"
                     /protein_id="HRT91151.1"
                     /translation="MIAWSTPQKPAELTESRLISAILDGNFPVNSPLPPERELALSLG
                     VTRPTLREALQRLSRDGWIEIHQGRPTRVRDYWQEGNLLILSAIARHQQHLPADFVPN
                     LLHVRALLAPEYTRLAIRNASSEVIRLLESMQTLPDAAPVFTRVDLELHCRLTILSGN
                     PIFTLILNGFTDLYHSMGSRYFLSPLTRDHSRSYYKKLLSAALSNDIFMAEEVTRAVM
                     RESLELWQEIEGQTSS"
BASE COUNT        14984 a        17477 c        19169 g        14802 t
ORIGIN      
        1 ataccatgca aaaaaacgaa aactatgata accttaacca gaggttcgaa gaatggctga
       61 aagttcactg agcaacattg acccggtctt cccctgccgt gaatgtcagg cggggcatat
      121 gcatcgtgat tacatcgctt acttcacctg gttgggggat gagctcattt ctgtgcctga
      181 tttccctgcc tgggtgtgtg acatctgcgg gcgatgcgaa tatgatgaaa atgcgctgac
      241 ccgcttgact ttactattaa gccccaacgc aggaaagacc atcgctcccg cccgccttcc
      301 gcgcacgaaa acgcgcgtta aacccaaagt gcaaagcccc actcaacctg actaaaaact
      361 cccggcacgc cgggagtttt ttttagggga tagggaaagg caccggctgc caggcgggtt
      421 ctttatcctg gctggcgagg ttggtaaggt tggtcacctg ctcgccgttt ttgtccatga
      481 tgtaaatttc tgcattgccg tcgcgcgtgg aactgaacaa cagccactgc ccatcaggcg
      541 accagcacgg ccagctatct ttgccggaac tggtgagcgc ggtgatggtt ttaccacgat
      601 cggcaaatgg tgcgacgtaa atattggttt tactgccgct cgtttgcgtg taggcgagca
      661 gggcgccatc caggctcaga ttgggttgtg aaaccgaacc cagccgtccc tcgtcagaca
      721 tacggtcaaa tttatgttcg ccgttggtat attgactgta tttatcgcgc atatacacaa
      781 cattcaggtt gcctgtcgat tgaacaaaca gcaggaactc cgcgtttggc gtccacgtcg
      841 cgtagctttc agcgaaaggc aggctcagcc gggtttgttt ttctccctgt gggtccgtca
      901 tgaataactg ggggatcccg tcatcacgat aataactggt atacacgatc atgtcgccta
      961 caggcgacca ggaggggtat ttgtcatcgc gctgacgcaa actgatatcc accgcttgcg
     1021 catcgggaac tgaaagatcc agcaaccaga tatccatagc caatgggttg ccattggcgg
     1081 taaacccttt tgaaaaacca ctatacaaca atttgctgcc atctggagac cacgcggggt
     1141 tggctttgtc tccttcgcta aaggtcagtt ggacgggatt gaccccaacc agttgacctg
     1201 actcgttttg catcacatcc atcagccaaa tctggtcaaa accatccgcc tgacggttgc
     1261 tgacgaacgc cactttaccg ccttcgccaa tggaagtgta gaccggcgct tcagtttcgg
     1321 caggcagagg ttcagcagtg gaggttggtt ctatgttctg cgcggcggtc ggttcttcgg
     1381 tggcgggcgc gggtgtctcc ggtgattgcg cggtgatcac aatgaccatg ggcgtaaggg
     1441 ttggcggagg ttcgttcgaa gaagtgcccc gcagagacag gttgcgaaga accaaaacgg
     1501 ccgcgatgcc caaaccaagc actgccagcg cgccgatgat ccaaccgaga ggagatttct
     1561 tttttgctgg cagcgctggc gcaacctgag ccggaggagc aacggatgga gggggtacga
     1621 gcggagcgcc gcgtccaaca gctgcaaccg tgcgctgtcc gaccagggta ttctgagaag
     1681 aagagggatc tgggatggga gaggtattgc aaagagacac gatgaaatcc tggacagtgg
     1741 aaaaccgcgc ttcaggtttg atggagaggg ctttttcaat ggcagatgcg gtgtgcgcgg
     1801 gaatggtggg attcaattgt gtcagtgggt tcagttcttc ctcgccaatc atgcgcttgg
     1861 cgctgtcagc agggggtttg ccggcgaaca ggtaatacaa tgtggcggcc aggctgaact
     1921 ggtctgaata tggtccggtg cgcaaaccgc tcacctgttc aggtggcgcg taaccaggcg
     1981 tgtaccccca ggcgccggat aaggtttctt gagaggcttc accggtttta gcaatgccaa
     2041 aatccaccag aatcacttca cctttggggg tgatcttgat attggcgggt ttgatatcgc
     2101 ggtggtaaat gggcgggttc tgtgagtgca ggtagctcag cgcattccct agctgctctg
     2161 cccactgcag gatgagcggt gtggaaagcg gtgtcttctg ttccacgatg gtgcgcagat
     2221 tctccccggg gataaaatcc atcaccaggt actggctgtc gttttctgtg aagtagtcga
     2281 tcacgcgcgg aaggttaggg tgtttgagcg aggccagcag acgggcttca cggatgaatt
     2341 gcgcgctggc gttagggctc aggttgtggt tgaccttgac ggcaacctgg tttgccaggc
     2401 tctgatcttc tgcgaggtat acttcgccca tcccgccttt cccgagcagg tcaagaacct
     2461 tgtatcttga tttcaaaatg aaaccgggat cgatatctgt cataagaagg ctttctttgt
     2521 gtgattttta ttttattaat gatacacaaa aaatcaaaaa acgtgccggc agaatcgcgt
     2581 ctttatcggt tcgatgacgg gggtcatact tgctgctcgc aagataatgt tcaaccttag
     2641 taagtttcct gggtgagggg tgaggataag ataaaacccg ggcagccaca cgctggaaaa
     2701 ggcccggggg ttggttgaga ggttgttaca ctgaggatca gtttatttgg cggctggttt
     2761 cttttctttg gatttcttgc gccttaggag atacccgatc acaaaaaatg ccaccccaac
     2821 cagtatccct gccagctgga cgttattgag cccggggtag gtaccaatac cgagggtgtc
     2881 agcgagcagc gacaccgcaa caatgaccaa gccagcgatc ataccaatga gacctgaagt
     2941 ttttctgaac aaggcgtcct ccttacaaat agatatgtat atcatagcac aagtcacaag
     3001 cagcgataat cacaagattg taaactgaaa aacagaaatc ttgtccctgt aatcaaaaag
     3061 aaggcgtctg ctggttcttt tgaaaaaggc tggttgacca ttttcaaaaa ggaagaaccg
     3121 tcatgaggtg cgcgaacgga gaagaaaacc ccctgcaggt tgatggacgc gctggcatgc
     3181 agcgaaccag gagcaccacg agtatttatt aacgtaattc acacgaagaa tgctaaaccg
     3241 cgaccgacct ttccggtgcc tttcagttgc gctcctgggt gaactggcac tgatattgca
     3301 aggattcctc cggaaacaag gtgcctgaat gaacgggtta cctgtacagg tggtgcgata
     3361 tgggtttgaa atacaatgat tttgcgatac gcggaattga cgtcagcgag ttcaacggtg
     3421 cgatcaactg gtccaaggtg cagggacact tcgctgccat tcgcgtgggt catggtcgtg
     3481 ttatagacaa gcgttttaac gcaaactggg caggcgccaa aggcaaggtt gaccgcctgg
     3541 cctactggta tatggattac tacagccata aagacaagtc caccagcgcg tatggcatca
     3601 gcgataaaga ctgggggcgg gtgcaggcgg aaaagtgctg gagcgccctc aaaaatgacc
     3661 cggaaggcat ggtcttttta gatattgaga aatccagtta cggaccggcg ctttcaaccg
     3721 tgcaaccacg ggtgctcgcc atcgcgcaat ctttttttga tcattacgat aaattttcag
     3781 gcaaactcaa cggcatctac tgctcgctca gcatgtgtac ttactttaac gaggcattgc
     3841 gcaaacgccc gctgtgggtg gcgtggtaca acgaggcgca gaacattgat tcagtgaaga
     3901 aggcagtggc tgcccgcggc tggctgggga agtgccttat ctggcaatat gccagcgacg
     3961 gagacgtgaa tgatgatggg aaagccgacg gcaccagcct gggtatggcc tatggcttcc
     4021 tcgacttaaa tggctggctg ggcacgaacg ctgaatatca gcaattattc ggaaagaagg
     4081 ctgaagtgga actctacaaa gtgaaaatat tgatttacaa cctgttggtg agaaatgggc
     4141 cggggctgac ctataaccgc ctgcgccggg ctgattaccc tggagaatac agcatctacg
     4201 aagagagaga cggctacggg cgcatcagtc agacgctctc tgagtggatc agccttgacc
     4261 cggcttatgt gaaaaagtta ggcgctgccg cggttgaact aacggacgct gagaagctgg
     4321 cgcggctatg ggcggctcac cccgaattac actgatgggg gtataagcgt aaacatctca
     4381 cctggattgc ggaaacctgt gagtatgaaa aaagactctt tcgagtctct tagagcgggc
     4441 gatgggactc gaacccacga atatcagctt ggaaggctga cgccttacca cttggcgacg
     4501 cccgcatcac gttcaggatt ataaaccaac cttaatgaat gggcaaggaa aaaaactggg
     4561 agggcgcgcc ctcccagtag gacattatga tacaagctcg cagtagcgtt ctacacgctt
     4621 ctcactcacc ctcaggctgt cttcccagaa cttgcgtgtg cgaatgtcaa taccgaagcg
     4681 cgctgccagg tcggctgcag gggcttcccc ggtgctcgcc agtagatgga tgtaatcagg
     4741 cacgaattca gcgccgcgct gctgatagat ggcgtacaac ccggtagcga aaagatgccc
     4801 aaaggcgtag gggaagttat agaatgaaag gccgccagaa tagtagtgtg gcttccatgt
     4861 ccacatccac ttttgcaggt atctttcgtc aagtccatcg ccgtaagtgg ccttctgcgc
     4921 gttctccatt atctcgcata tctcatccgc gttgagctcg gtcttctccc ggcgttcgaa
     4981 tacttccttc tcaaacaggt aacgcgagta gatatccacg atcacctgcg aatcaccgtt
     5041 caactggctt tcaaggatgg ccagctcttg ctgcgggttg gtgacctctt ccaacagcgc
     5101 cgaggtaacg atggtttcgc acatgatgga agcggtttca gccagcgtca tgggagtgaa
     5161 cttctggatt tcggtcttac cggctttgac cgcgcagtca ttgtggaaac catgccccag
     5221 ttcgtgcgcc agtgtggaaa cctggtcaaa tgaaccgtca aaattggaga gaatgcggct
     5281 ttcacccact ccgggcactt ccatgcagaa cgctccgccg cgtttaccgt cacgctgttc
     5341 cgcgtcgatc cagttgtgtt caaaggcgtt gcgggcgaag gcttccagtt ggggggcaaa
     5401 acggccaaaa ttcctgagga tgacctctct ggcttcttta aacgagtaat ccgatttgac
     5461 ctcaccgacc ggagcgaaca ggtcccacca tgggagtttt tccttgccca ggtatttgac
     5521 cttggcttta aagtaacgcc ggaacatggg aaaagaatcg cgcatcgcgc ccagcatggc
     5581 ttccagcgat tgccggtcaa tatgcgccgc gtcgatggcg ctgtgcaccg cgtcttcacg
     5641 cccgcggcgg cggttgagca cgtttacttc gcccttgatg ctgttcagag cggcagacag
     5701 agggaccttc agtgaggtcc atagtttgtc ttctgcttcg taaccgcgct tgcgcgtttc
     5761 gccatccggg tgcgaacgaa ggttgatgag cgccggtgag ggcagccttt ttaactcgcc
     5821 gtctacttcg atctcaacac tggcctgcga ggtgagcatg ccctgcagtt ttgtccaggc
     5881 gttggaaccg ctcaggctga gctctgcggc cagcatttct tctgcttcgc tcatcaggta
     5941 gcggctttga cgggcagatt cttccaggaa gtaacgatgt tcttccaaca ctggcacgga
     6001 ctggatcatc tcaggaagac gttcctttac cttgcctatc cagcccagca actgggtttc
     6061 cagctggttg atcttcactg ccaccaggtc aaattctgat tccttacgca aggcttcctg
     6121 gttatatgaa tctgtagaaa cgaatgattc aatataggcg cggatagtac cgccaaccaa
     6181 aaggatagcg ttgtagcatg cgatcatttc ctgcgcgagc gcgccgagcg ttgaagcatc
     6241 ttcctggccg ctgaaagccg ccgctttttc catcaggctt tccatatccg agatgaacga
     6301 tttcaccttt tcgcaatcgg cgttgtattc tttcgaggaa agggaggggt agacgttggt
     6361 caggttccag cgtggtgcgg tttgtgccgt catgtgggct cctttgagtg agaattaaca
     6421 cgttgattat atgccaaaaa cggctagaat taggcaacac gaacaagata aggaaaactt
     6481 ggtgccataa aaatccgtat atgtaatata aggcaacgat gaagaaagga aatgatatga
     6541 gtgaacaatc cttcaaccag gaaagaagag aagtcagcgg tttttcggcg ttggtcttca
     6601 gagggatggg gagaatcgac ctcctccaga gcgatcacga agagttggtg atcgaaacgc
     6661 agcctgaaat acgttcacgc attaaaacgg aggtgcgtga gggaacgttg tacatcgatt
     6721 atgatgaaaa ctggaaagac tggaccggtc tgcgcagcct gagtggagac aagatcgttt
     6781 tcaaactgat gatgcgtgag gtgacggcga tttctatttc cggtgtgggc agcctggaca
     6841 cgccgcgcat tgagagcgcc aatctttcat tggccatcac tgggccgggt ttactgaccg
     6901 ttggaacgtt gaaggtgccc acgctgacgg tgaacctttc gggagttgga gccattgacg
     6961 tcgccggtca gacggatgag ctgagcgtta ccctcagcgg agctggttct ctcaaagcca
     7021 gccggcttga agccgggaaa gccagcgtga ggctcagcgg cgtaggaacg gccaccgtgt
     7081 gggcaaaaaa cagccttgag acttcgatct caggcgccgg ggtcattgaa tattatggca
     7141 gcccgcagat aacgcaaaac aataccggcc tgggtgtgtt aaaattcatg ggcaaccgtt
     7201 agcctgatag gggttgtaaa taacgatttt ggagtattca atggcacaag atagaactcg
     7261 taagattgtt gtcaccgccg tcctcggagc gatcaccatc ctgcttgggt tgacgcactg
     7321 gggttttatt ccatggtttg ggggtatttc cctcaccatc atgcacgtgc cggtcatcat
     7381 cggtgccatt ctggagggtc cgttggtggg agctggtatc ggtctcatct ttggtctgtt
     7441 cagcatgctt caggcggcga tcgcccccac gggtccgctt gaccccctgt ttaccaaccc
     7501 cttgctggct gttttaccgc ggctttttat aggcccggtc gcctggttgg tgtggacagc
     7561 gttgaagaag tggccggtcg tggggttgat cgccagcggt atcgccggca gccttaccaa
     7621 cactgttttg gtgttaagcg tgctggggtt tttctttggt cgcgcgccag tgatgaccca
     7681 gatttttggc gaagagctgt ggaaaccact gagcggaatc gcggtggtat caggcctgcc
     7741 tgaggctggc gtttcagcct tgattaccct gctggtggtg gcggcttacc gccagttcag
     7801 cgtggggaag cgcaaaggcg ccaacttgga ataaaccgcg taaaaaatac ggcagcgatg
     7861 gtctcacgcg aagaagactg cgagtgagac cactacgctt atcaagacgg ccaagccatc
     7921 ccggaatgag aaatgcaact cagtcatact ggagcggtat ttaagcacgc cataagcgcg
     7981 ggcgtccatc gccagcgcca ggttttcaga gcgccgcaga ctggtgataa agagggggat
     8041 gatcatcggg atcacctggc ggatacggtt gaacagccct ttacgttttt gtccccaatc
     8101 tgaaccgcgt gacgcttgcg ctttggcaat acgttctgct gattgcgcca ggaagggcaa
     8161 gaagcgcagt gtcacctgca cgatcatcac aaagtccatg gtttgaatac caacgcggtt
     8221 gagagggctg aggattttcg tcaacccgtg cagcagctca gatgttgagg tgacgaagct
     8281 caccaggctg aggcacagaa tgagcccggc gaaacggatg agcagcatga cccccgccag
     8341 cagaccgccg gaggtgatat tcagttttcc cagcttaatc aggatgttcg ggtctaacgc
     8401 gctggaatag aaaaacacct gaatgatggc gatgatcaaa aggaaaggca aaggtacgag
     8461 caggccctta agcgcaaacc cgatggggac tttggaaagg agcagcgcca gcaggagcgc
     8521 ggcgatacca atcgacagcc cgagttgtga aggggtaaaa gtgatggcga tgagcagcac
     8581 gccaaatatg atgattttcg cgcgcgggtc aagccggtga atgatagaat tcgtagggat
     8641 gtattgaccc aggggaatgc gcgagaggaa atcaaattca ctcatgcgga ccccctgtat
     8701 tcactgcgcg caacgcctgg aataactgct cggcccgcac gacgcgctgc gggattggcc
     8761 atcccttttc ccgtaaggct cctgctaatt tgacaattga cggctgcccc agcgaggctt
     8821 cgtcgatcaa ccgcgcgtca ttaaacaggt cagcggtatt accggtgcgc aacgctttcc
     8881 ctttggaaag gatggtgagg ttttcagtca gttcggtgat atcgcccatg ctgtgcgagc
     8941 tgagcaccag ttccacgccg ctttcctggt aatccttgag gatgccatgc agattgcgcc
     9001 tggacgccgg gtcgaggccg gctgtgggtt cgtccaggat gagcagacgc ggttgcagcg
     9061 ccatggtagc cgccagcgcc accttgcgtt tctcaccgcc actaagcgta taggtaaggc
     9121 ggtctttgaa tttatcgaaa tccagaccca ccagctgcat cgcttctcgc acccgctttg
     9181 ccaggttttc tttgccgtac aaattcttgg gaccgtaagc gatttcatcg cccacatact
     9241 gctcgaagaa gtaaatctca gggttttgga acaccaaccc ggcgaaacgc cgcaccgctt
     9301 tgatatcaag acgcgggtcg cgaaaatcgt acggaccgat gcgcagtttt ccgctttgcg
     9361 gacggtagag cccgttgaga tgttgcagca gggtggattt gcccgaacct gtggcgccgc
     9421 aaaaaccgtg agccgcgccg ctatgtactt gcagcgttac atcgaaaagg gattggtgcg
     9481 aaagcggggt atcaggcaga taagtatggc tgagattctc cacatcgatc aggatttcac
     9541 ctgtcatggg tgaaaggcgc tcagagagaa gctggtgtga accgcggtag ggtggtattt
     9601 gcgccagcgc ctgttcaaaa tcaaccggca aatggttcag ttgagcgtta agctggcgaa
     9661 tttcgcgcag cagcatcacg gttgagggtt gctccagccc gcaggcagta agcagcttca
     9721 ggtcagcgaa aaggtcctgg ggggcaccgt caaacaccag gttgccggca ttaagcgcta
     9781 aaacgcgctc ggcgcgcgcg gcttcttcca taaagtgggt gatgaacagc acggtgatgc
     9841 cgcggttgtg cagcgattgc atttcagcca gcacatccag cctgccgcgc ggatcgagca
     9901 tagcggtgga ctcgtcgaag atgacgcatt ccggctgcat ggccagcacg ccagccagcg
     9961 ccacacgctg tttttgacca gccgagagca ggtgcgggag ttgttcttta tgaccgctca
    10021 tttttacggt ttcgagagcc gcgtccaccc gcgcgcgaat ttccgcaggc agcacgccca
    10081 ggttttcagg tccgaaagcg gtatcttctt cgatcagcgt ggcaacgatc tggtcttcgg
    10141 ggttctgaaa aaccatccct acccccgcgc gaatcttagg gaggttgctc ttcagcgcgg
    10201 tgttttgccc ggcgataagc acctgtccgt gactgggcag cagcaacgcg ttgcaatggc
    10261 gcgcgaaggt ggtcttgccc gagccgttgg cgcccacgat ggcaacccat tcaccctggt
    10321 gaatttttac gctgatatcg ttcaaggctg gcggggagga atgttcacta ccgggatggc
    10381 ggtaggtgac gtggtcaaac tcgatcaaag gcggcaaaac gcgctccctg aaaaagtgat
    10441 acctgcaaac acgcggttat tatactggaa gaaaaccctt acacttgaag atatttcccc
    10501 ccatttttgc ttgacttcct gttcagttgt gtattcttat tcctgttata taataagtgt
    10561 aaggtttcaa cgtagaataa atttaaacgg aggaaataat gaaacgcctg atctaccttg
    10621 ctgtggtgct ggttctggtt tttgctcttg ctgcgtgcag cgcatcttcc gatcacctgg
    10681 cggcaatcaa aaaagctggc aagatggtgg taggaacgtc tgctgactat ccgccttttg
    10741 aatttgtgga tgaggcgggg aataccaccg gttttgacgt ggatttgatg aatgagattg
    10801 ggaaacgcct gggtgtgaag gtggaatggg tagatatgcc cttcgatagc ctgatcgccg
    10861 cagtacaggg cggtaagatc gacctttcca ttgcggcttt caattatgat gaagaacgtg
    10921 acaagacagt tgatttcacc gatgcttact acaccagtga agacgcgttg gtaatcatgg
    10981 aatcactcgc aggcgacccg ccaactcctg aaaacctcga gcagttcatc atcggggttc
    11041 aatcaggtac tacccaggat gcctggttga cagataacct cgaagccacc ggaaagctga
    11101 aagcgggttc cttacagcgt tatgagcgtg tggaccaggc ctttatggac ctgcaggctg
    11161 gccgggtgca ggtgatcatg tctgattatg tgccagccaa agcactggtt gataagttcg
    11221 gcgggatgaa aatcgtctac cagggtgtgc tttcgactgg tccgatgaac attgtcattc
    11281 ccgatggcga taagcacctg caaactgagg tcaacaaaat catcaaagac ctgcaggatg
    11341 aagggttcat cgacgcgctg gctgtcaagt atttctctga gtaattttct cggcattcaa
    11401 acggaggtaa cggtatcagt gacctgatta ccgttacctc gaccacaacc gctgcgcttg
    11461 ttcacttata gcgctgccta tcctcaacca ggagtgattg agcgttggat ttcatgaagt
    11521 tcctgcagta tattgcttcg ggcaccgctt atacaatcgc agtcacgttg gtagcgttgc
    11581 cttccgggtt gctgctggga atcctcttct caattctgaa cgtttacggg ggaaaagtta
    11641 ctcagcgcct gacctccctc tacagcacca tcatgcgtgg tatcccgccg attgtgctgc
    11701 tctttattct ctatttcatc gtgtctggca gtatcaacct ttcaccgttc tgggcgggtt
    11761 cgatttcgct gggaattgtc agcagcgcct accagatgga gattgtgcgc ggcgcgatac
    11821 tatctgtcag tggcggacag atgatggctg cccgggctat tggcatgagc aaaaaccagg
    11881 ccatcaggca tgtcattttg ccgcaaaccc tgcgcctggc catcccgccc tggtcaaacg
    11941 aggctgccat cgtggttaag gattcttcac tggtatacgc tttgggcgta ccagaaatcc
    12001 tgcggcgcgc tcagctgatc agtgccagca cccaggaacc tttcttggcc tatgcggtgg
    12061 ctgcgctcat ctatttcttg ctggtgttca tcaccaaccg tttgctggac gcgctggacc
    12121 gacggttgca tattccagcc aacccatgat ttggagacag acatggcaaa accgattctg
    12181 accatcaaga agctgaccaa aaaatacggg gaaaacgtca ttttccatga tatggacctg
    12241 gtggtgaacg aaggcgatac catcgtcatt attggcccca gtggaacagg caagagcacg
    12301 ctgctgcgct gtattaactt gctcacaatt gcggatgagg gccagatctg gctggatgat
    12361 atagagatta ccgccaaaag ttgtgatgaa gacaaggtgc gccagcatat tggaatggtg
    12421 ttccagaatt ttctgctttt caaccacctc accgcgttgg aaaacgtgat gctgggtctg
    12481 accaaggtca ggcacatgaa accggatgaa gccaaacaca aagcgttgct cgaactggag
    12541 cgggtagggc ttttagaccg ggctgaccac tacccggggc agctctcggg tgggcagcaa
    12601 cagcgcgtgg cgattgcccg cgcgctggcg atggacccgc gcattatgct tttcgatgaa
    12661 ccgacctcgg cgttagaccc tgaattgatt ggtgaggtgt tggaagtgat gcgcaaactg
    12721 gcgctcgaag gcatgaccat gctggtggtg acccacgaga tggggtttgc cagagaggtg
    12781 gctgaccgca tcgttttcct tgaaaaaggc agtattgttg aagaaggttc accagaagac
    12841 ctgttttacc accccaagca cgagcgcacg cgtgaatttc tctggaagat caccgaactg
    12901 tatgggaaga aagaaaaaga gagttaaatg atcgcctggt taaagttgtt tgagcgctat
    12961 ttacccgaat tcatcaaggg tgcgggggtc acccttgagc tgaccgcggt tggactcacg
    13021 ctggggtttg ttctcggtct cgcgctggca gtcgccaggg tatatgcacc gaaatggttg
    13081 aaaaccattg ccatcggtta cattgagctc ttccggggta cgccgctgct ggtgcaattg
    13141 ttcatcatct attacgggtt gccttcgctg gggattactc tatcgcaaac cctttccgca
    13201 tttctggcct tgggattgaa tagcgcggct taccaggcag aatatttgcg cggcgcgatt
    13261 ttatctattg gggcgtcgca gatgaccgct ggcagagcga ttgggctttc gcgctggaag
    13321 accatctggt atgtcatcct gccgcaggcg ctgcggttgg tgatccccgc ctggtccaac
    13381 gagccgattt cgctgctgaa aaccacggcg attgtgttcc tcatcgcggt gcaagacctg
    13441 atggcaaagg ccaaacgtgc cgctgcggat acctacaacc cggtaggcag ttacctggcg
    13501 gtcgcggtgg tttacctggt attggtgttc atattcacct gggggttgaa gtacctggaa
    13561 aagaagaccc gcatcccggg ttttgagatg ggattaaaaa agggctgagt aacgctcgac
    13621 cagttccata aacgtgggta gattaatatt gctgccactg atgaccactg tggttggggc
    13681 atctggcttg attttgcctg ccagcaaggc ggcgaccccc accgcgcccg aaccctctac
    13741 caccaggcga tgtttataaa aaaggtaggc caccgcctga ccgatctctt cttcgctgac
    13801 cagagcggta tggttgacca cttcacggca aatcgcgaag gaataaaggt tgggggtgcc
    13861 cagcccgcct ttcagtccgt cagccagggt ggcttcttct ggcaactcca atactcttcc
    13921 tgcttccagg ctgcgcacca tgaccggagc gcgttccatg cttacccccc aggtttgcac
    13981 cggcgaagat tgctgccggc tgacgaggcc gattccggcg cccatgccgc ccccggagag
    14041 cgggtagata atgtggcagg tgtctggcgc atcggcgaga atttccagcg ccatggttcc
    14101 ctgcccggca ataacccgca gatcgtcgaa tgaagagacg tgcacaaggc catttttttc
    14161 ttcgtattca atcgctgcgg catcggcctc atcataacta ttgcccgtta cgataatatg
    14221 cgcgtgcgtc ttgcgcagac tctctacttt gcaggcgggt gtatgggtgg atagaaaaat
    14281 ggtgaccggg actcccagta attcacccat gtacgccagc gcctttccat gattgccgct
    14341 ggaaaccgtg attacgcccc gcgttttctc ttcttcggtg agttgcagca agcggttggc
    14401 agccccgcgc agcttgaatg aacccgttgg ttgcaaacac tcaagcttga gtgagaccgg
    14461 ggcgttgact ctttcgctca gaaaaactga atggatcaaa ggtgtgcgca ggatgtaagg
    14521 ttgaatccgt ttatgcgcgt ctttaatatc ttgaagcgag atgagatcca ttggatgtgt
    14581 catagaacct atgacttatt ttatatcaaa tagcaagaag gacctgtaaa cttaattgag
    14641 gaaaatcctg ctcagggata attattaggg acaacccccg agtaaggcgc gaaagcctga
    14701 cttctcgcat aagcaggagg aaccaatcct ttgaaggagc caaacgcgaa tgacggtttc
    14761 ttacgatacg gaggacaaaa atcgttgatt ttgatgggta ccagataatt ccttttctgg
    14821 gctattcagg aaacggtttt acaggccagg taaagggttg gcagcggaag gatttgaacc
    14881 ccgtgatagc ggcaggcagc ctgcagcgcc tttggcagcc aatgctggcc ctcacatcgc
    14941 agccttgaca ttcatccttc acccgtctat actcaatgta gataaagcaa cggggagccc
    15001 gctgcacggg ctgagagtag ggagcgaacc ctagacccgc attacctgat ccggattatg
    15061 ccggcggagg gataatcact caaccataca accctccctg gcggagggtt gtttaatgtc
    15121 aattaaccac gtgcttgttt ttgccaatgg tgaactgatg gacccgcgag cagcccgtga
    15181 gatggcgact cgcgcggatt ggatcatcgc ggctgatggc gggctggctc acgtgcaggc
    15241 gctcaacctc aaacctgacc tgttggtcgg ggacctggac tcggtatccc ctgagcagat
    15301 acgctgggca gaaaagatgg gcgcagaagt acgcaggttc ccccttgaca aagatgaaac
    15361 agaccttgaa ctggcgctgc aagaagctgt cgcaacaggg tgcagccgca ttaccatcac
    15421 cggcgcgctt ggcggaaggc tcgaccagac gctctcgaat atctacctgc tcaacctgcc
    15481 agtgctggct gaccttgatg tgcgcatcga tgacggcgcg caggaagtga tcctcattca
    15541 tagcgcggtg gcgcttcagg gcagcccggg agataccgtg tcattgctgc cgctctcgcc
    15601 aatcgttcgc ggtatttcca ccagcgggct gaagtacccg ttgaccgacg aaagcctgat
    15661 tttttaccgc agccgcggga tcagcaatga aatgtcggct gaaaacgcca atgttcagat
    15721 tcaatcaggt atcctcttat gcatccatgc gcgacgcgag gtgaccctat gaaacgccgg
    15781 ttatgggtat gcgctctgct tggcgcgctg ctgatgaccg ccggttgtgc gcgggcggtt
    15841 gcaacagaaa ctgcgccacc ggtccttacg gtgatgacgc acgattcttt ttcagtctct
    15901 gaaacggtgg ttaaaacgtt tgaagaagag aatggcgcta aactggtgtt cctaaaatct
    15961 ggcgataccg gcgctgcgct taaccgcgcg attctcaccc gaagccatcc tgaagccgat
    16021 gtgttctatg gaattgacaa tacttacctc tcgcgcgccc tgcaggagca aatttttgtg
    16081 ccatacacac cagccgcgct cgcgcaactg ccgggagaat ttaaaagtgg gctttctggc
    16141 atggtgacac cgattgactt tggcgatgta tgtatcaatt acgataaagc ctggtttata
    16201 gagcgcggat tggcgctgcc agcaaccctg gcagacctgg caaaacctgc gtataaaggt
    16261 gaagcaggga acgggttgct ggttgtggaa aaccccgcta catcttcacc ggggctggcc
    16321 tttctgctgg tgaccatcgc ggagtatggg gaaactggct acctggatta ctggcgttca
    16381 ctgaaacaga atggcgtggt agtggtgaat gactgggaaa ccgcttacta taccaacttc
    16441 tctgcttctt cagggcgcgg gttgcaacct atggtggtga gttacgccac cagcccggcg
    16501 gctgaggtca tttatgcttc tgagccctta aacgaagcac ccactgcctc catcaccact
    16561 gcgaacacct gttttcgcca gatcgagtac gctgggatct tgaagggcac cagtcaactt
    16621 gaactggcac aaaggtttat tgattttatg ctcagcccgg ttttccagga agacctgccg
    16681 ctgcaaatgt ttgttttccc agttaaccca gcagccacgc tgccagatga ttttttgcaa
    16741 tacgcagcgt tcgcgcagga gcctgccagg attgaccctg tgctgatctc gaaaaaccgg
    16801 gacaagtggg ttaccgagtg gaccagggta atgctgcctt gaggttctgg caggagttga
    16861 cgaggtcagt caagcaatct ggcgtgctgt tatggggagc gccgttggca ttgttagcgg
    16921 tcttcttttt tatccccatg gcggccattc tgaacgtcat gttcgcgcaa agcggggata
    16981 ttgtattctc tgattcgctg cgaccgctgg gttttactgt ttttcaggcg ttgctctcta
    17041 cattgttaac ccttctattg ggattgcctg cggcgtatgt ttttgctcgt ttcgatttta
    17101 caggcaagcg tggcttacgc atgctcacca tgctgccgtt cgtgttgccc accgtggtca
    17161 ccgcggcggc gtttaacgcg ctgctcggac cgcgtggctg ggtcaacttg cttatgatga
    17221 agttgaccgg cgctcaagcg ccaccgataa actttttaaa tacttttacg gccattttgg
    17281 tggcgcatgt tttttataat attgccatta tcatccgcgt tgtcggtaac gcctggtcac
    17341 aacttgaccc gcgtcttgaa aacagtgcca gggtactggg ggcttccccg tggcgcgcct
    17401 ttattgaagt cgtttatcca ttactgcgcc gtcctatcct ttctgccgcc ctactggtat
    17461 tcctttttga tttcaccagc ttcggcgtca tcctcatgct gggaggggct ggtttttcta
    17521 cgcttgaggt ggccatttat actcaggctt tgcataacct caacctgccg atggctggtc
    17581 tactttcact catccaactt gcctgcacag tcatcatcct gctcctttat ggctggtcca
    17641 gccagggtaa cgatgtgccc ctctttccgc gcttaagcgg agaaggtacg cgcaaaccac
    17701 gttcagcggt tgaaaaaggg accgttgcgc tgatggtaat tattttattc accctacagg
    17761 tgctcccttt atttgcgctc gtcagccgtt cgctggtccg ccttgaaggt gagcgcggtc
    17821 agcgtggaaa ggtacaatcc gggttgaccc tggattacta ccacgaactt ttcgtcaacc
    17881 ggcagcaaag tgttttttat gttccgcctt cgcgcgcggt gattaattca ctgagttacg
    17941 cgggtataac catgcttctc tctgtgggtg ctggcaccct gctggcttac gcgatggtga
    18001 aacgcccagc cacaaggcgc tggctggatc ccctggtaat gctcccattt ggcgcgtcct
    18061 ctgtaacgct tggcttaggg ttccttatcg ccttcagcgg cagcatgctg tcacgcgcat
    18121 cttttccctg gctggtaccg ctggctcaca gcctgatcgc catcccgttc gtgttgcgta
    18181 ccgtacagcc tgctttgtct gtcatgccaa aatcgttgtg ccagtcagca gcggtactgg
    18241 gtgcatcccc ggcgcgcgcc tggtgggagg tggagatgag gttgctgagc cgccctcttc
    18301 tggtaggcgg gctttttgcg ttcaccatat cgattggtga gttcggcgcc acaaccttta
    18361 tcgcgcggcc agacaatccc acagtgcctg tagccatttt tcgttacctg gctcagcctg
    18421 gcgggttgaa ttacgggcag gctatggcga tggctacaat tctgattgtc atctgcgcgg
    18481 tgagcattgg tttgcttgaa agcctcagcc gcgcaatcaa cggcagtgaa taacggaggg
    18541 cgcatggacc gtttggaaat catcgatctg cataaaatgt atgaaggcgc ccctctcctc
    18601 gatggcgtga gtttttacgt ggtggctggc gaaaccctgt gcctgctcgg cagttctggc
    18661 agcggcaaga gcaccatttt gcgcattatc gcgggtatcg aaacccagga cgctggcagc
    18721 atcctctggc gcggcaagga tgtcagcgct gtcccggcgc accggcgcgg gtttgggttg
    18781 atgtttcagg attatgcgct tttcccacac ctcaacgtaa gcgagaatat cgcttttggt
    18841 ttgcgcatgc agcacgcagc caaagatcaa cttggcgccc gtgtttttga agcgctggag
    18901 cgtgtcaata tgactgcttt tgccagtcgt cgggtcactg acctctctgg cggcgaacag
    18961 cagcgtgtgg cgcttgcccg cgcgctggcg ccgcgaccac agctcttaat gctggatgaa
    19021 ccgctggcag cgctcgatcg aagcctgcga gttgagttac aggaagattt acgcgagtta
    19081 ctccatcaga caggcattcc tgctatctac gtgacacacg accaggaaga agccatcttg
    19141 ctgggtgacc ggctcgcctt attgcacaac ggacgaattg tgcaaagtgg tctcacagaa
    19201 gaagtgtacc gtcagccggc gaacacctgg gtagcgcgct ttttaggtat gaccaacttg
    19261 ctcgaaggca gggtggtcag cgtaaacccg ctgatggtac aaaccgcctg tgggacgctt
    19321 ggcccgttcg ttcctggata tccctccctt ttaagtccgg gtcaggtggt gcaccttctg
    19381 gtcagactgg atgggctttt ttcaggcgaa catcaacctt cagccggcag cattaacggc
    19441 gaggttacgg agtgccgctt catgggagac cattaccggg tgcgtatgaa ggtcggtgag
    19501 tgcgccagtt tggaatgctt tactgcctcc ccgcttgtac tggggaaggc gcttgaatta
    19561 cgcctgaatg cggaagatgt tgttgtgctg cccgaataaa aacgcttcca ctggcagtaa
    19621 aacctacggc gctggcattc aaggggaaat tttctggaaa acccaccaca agatgagcat
    19681 gaacaaaaca atgccaatac agaaggagag ggtcatgacc agcacaacct gccatacctg
    19741 ccttttccgg ctggcaagca gttcctgatt tttcacatct ggcagttctt tttctggctg
    19801 ggtcactcta cgggttgggt ttctcgtttt ttcttacggt tatttgccca aagaataata
    19861 ataatgagaa cgattgtcag agtgatggcc acgatcaccg gtatgatcac ggagagaatc
    19921 gaaaccactg tcgaggtggc ttgttcacca atcgaagcgg gtacgttacc caaaccaccg
    19981 gtggcagcgt ttacggctgg tcgcaccgcc agagctttta cggcgtgtac cccgcctgaa
    20041 accaacaggc ccgcgatcaa gcccagtata gggttcacat cacccacctg actggaggtg
    20101 gctgcaaata caaccgcccc agcggtaggt cgaatgaacg actggatgac atcatttaca
    20161 tggttaacgc ctgggaatag atctgccagt atctccacga tggtcagcac gataagcacg
    20221 ccgatcaccc accaggaagt catcaggttc cagggttctt gcagtttgat cagattggtg
    20281 aatttggcga gcagcgagac gaccagcaga ggaatatagg cgttcaaacc ggcgctggca
    20341 gagaggccaa acgctgtaaa aatattgaaa atatccatta attcatttcc ttttcctacc
    20401 aggtttggct gagagagaag cggatgagat ccataagccc aacctgggtg atagccgtga
    20461 ttaaaccggc actcaatata cgaaaaccat cgcgaaaaag atgcagcgag ttttcctttt
    20521 ttaagatgat acaccaaagc aatgtgtaga tcatgcccag caccaggtag atggtcaggg
    20581 tggagaggat ggcgataggg taaaacagga tgggcacatc ggataccacc atcaccccag
    20641 cgagggcgca aaataggaat aaccaaccaa gctgctgcca gctatgccag actggctgcg
    20701 ggttcgggtt tgaccatagt gtctggtgcc ataacggcag caacacattt gccagcgtga
    20761 taccgaagag caacccgctg atcaacctga gctggttgga gggtgtatag acggttttaa
    20821 gacccaccag cggaaggctg gcgttgatgc cgtcaaaggc gaaaaaggcg aaaaagaaca
    20881 acagcaccgt tattttctgc ctggatggga aaccgctttt acggtctttt gagtacaggt
    20941 agaccatcga gatgaataaa cccaggaaag tgccggtgca gcgcgcgcat aacggcagca
    21001 ctttccctcc gacactaaag gaatggctgt cgatttggtg gcagaccgaa aaccctatcg
    21061 cgttcatctt gccttcgata cccgggggcg tgagcagcag ccacgcagaa aaaagcgcta
    21121 ccagcagcag caccaacgca gggtttagcc agcgaaattt ttgaggggta tcgtcttttt
    21181 tctcagacat agggcaaatt ataaagggga taggaggttg gagcaagcca tagtcggagg
    21241 gcagtgggtg accgcgcccg cttgacgcga atgcggaatt atgattaact taaccagaag
    21301 aacttgccat cctaacgatg aggaggtgat gtctacgatg tgtaccagtt ttgaacacag
    21361 cgctgcggca tccctcctcg cgatagagta gggaagccgc agcgccaaac ccccggagcc
    21421 gtcagcgtga cggctctgtg gttttcctgg attaattcac tctttaaccg ctgcaccctt
    21481 aaaagaaaag tggaaagcgc caccagcaca agttggcaat atcacctcaa gcgtttacgg
    21541 caagggcgct taaccgtacg cggcaagagt tgcgtatacc agtcaaccca cgcttcggta
    21601 tataatcagt tcatcaagct accaggaggg cgcatgccga cagtaaaaga aagatttatc
    21661 aagtacgcca ggatcaacac ggaatcgaat cgtgacagcg agacctgccc cagcactcag
    21721 gcgcagtttg acttggcgcg ggtgctcgaa tcagaattgc gtgaaatggg tttgcaggaa
    21781 ataagcctgg atgagaactg ctatctgatg gcttcactac cggcgaacac tgaaaagcct
    21841 ctccctgtta tcggcttcat cgcgcatctc gacaccagtc cggatatgag cggcgcgcag
    21901 gttaaccccc agattgtcgc cagctatgac gggggtgata ttgtgctcaa ccatgaactc
    21961 ggtatacaaa tgaagccgat ggacttcccg gaactgcaaa agtatattgg gcaagagttg
    22021 atcaccacag atggcaccac cttgctcggg gcggacgata aggctggtgt cgcggcgatc
    22081 atgtctgcaa tggagcacct ggtcgcgcac ccggagatca agcacgggaa aatccgtgtt
    22141 ggcttcaccc cggatgaaga aattgggcgc ggcgcagata aatttgatgt ggagaaattc
    22201 ggtgccagtt acgcatacac ggtagatggc ggagaaattg gcgaactgga atacgaaacc
    22261 ttcaacgctg ccgaggtgca ggttgccgta agcggcagga atgttcaccc tggggtggcg
    22321 aaaaacaaga tggtcaattc gattctactg gctatggaat tcaatgccat gctgccggtc
    22381 aatgagcgcc ccgaattcac cgaagggcgt gaagggttta ttcatttatt gggtgtatcc
    22441 ggctcggtag aaaacacccg catgtattat attatccgtg accatgaccg gatgctgctt
    22501 gaaaaacgca agcaatacat gatcgacgca gccaccctgc tcaaccggcg ttacggcgag
    22561 ggacggattg gactggagat taacgaccaa tactataaca tgcgcgagaa gatcgaaccg
    22621 gtttttgaga ttgttgaaaa cgcgcgtgat gccatgctcg cgcttggcat agagccgaaa
    22681 atattgcccg tccgcggcgg cacggatggt tcgcgcttgt cttttatggg attaccaaca
    22741 cccaacattt ttaccggagg gcttttcgct catggcaagt atgaagttct gcccactcgt
    22801 tcactagagc gcgcggttga agtgatcatc aagatcatcg aaatggccgg taaagataaa
    22861 taacccgctc gcgagagcgg tttatttatc aacccgttat tttgacgagg ttccgtttac
    22921 tgagccattc gtttattacg agcgcgccaa taacgatgcc gccgccactg agcagccgag
    22981 ggaggttggc gctttcacca aagacgagca gcgaaaccgc cacggcgagg ggaattttaa
    23041 gatcgttgaa gacagccagc gcgcccgcgt ttacccggcg cgcacctatg ttccagagga
    23101 agaaagccac accggaggcg acgaccccca ggtaaagcag ggtgagactc tgtttcgaat
    23161 ggagttcgag ggtattgaat ggcgtggtga acagcgtcgc cgccgcagtg atgaccgttg
    23221 cgcccaggta aagatagcca aatacatcct gatcttttaa ctctggatgc tgcgccatca
    23281 acctgcggta ataaatttgc ccaaaagcgt aacacaggtt ggaaagttgg accagcagaa
    23341 acccattcca cactgcgggc gtttgaatgg attcccattc aataatggcg gtgcctgcta
    23401 cggtgatcaa tgccacagcc aaatgcagca ggttaatatg tttcataaag gcgtcattga
    23461 tcagcgtgac gaagataggg gtgaatatgg tgaacagcgc gacttcgtaa gccttgagtg
    23521 ttctgaaggc ggccaggtag gcgatgtaca tcatcccaaa ttgcaccaat ccggtgagcg
    23581 ccagtttgat agcggttttt gtttgaattt tcttccactt gatgaatgga agaaaaacca
    23641 gcgcggcaat gaacaggcgc gcgaaagcca cgaagttaga gtcaacccct gtcaggttgc
    23701 ctttaattaa tccaaatgaa aaagcccaaa tgactgaaac aaccagtaag gtaaacatca
    23761 gattattcca gattctcctt ttttatcgcg gcaatcataa cacattcatg aaccagccta
    23821 taccgagttt tttacttcgg aactgtcagc agaagaaagc gtaacttcca atatctgccc
    23881 tctagtttcc tttaagggat gggaagaata caaaattccc tgctgagaag catcaccggg
    23941 gatgaaaaag aactggcatc agccagttct tttgttctga aaaagggaga ggatgaggtt
    24001 acctgagcgt gtctactttg cgcttcatgg gagcaagcac caggacgctg ctgagaatga
    24061 gcccgatacc cagcaacacc agcccgctgc cgacaatcgt catgctggtg ttaaacgcgt
    24121 cggcgtagtt tactccgccc catgtgtggg ttggtgccat gaagaactgg atggtcaggc
    24181 acagcgctcc ggcgacgatg gccgcggctc cgctggatct ctttccttct ttacgcggcg
    24241 ctttgctgta atctaccccg ttccagatgg ctggaatacg gaagacgagg aaaatgacca
    24301 gggtcaacac agtgatgtac acgatgccat ccactggcat tgatgaaccc ctcagcatgc
    24361 gcgaggtgat catatgaacc acccccacaa ccaggcctgc gaccagcgcg atcagcgccg
    24421 ctttgtaagc gccctgtcgt ccttttgcca gcaataccac tgccctgatg cccaacaccc
    24481 cgatggcgat ggtcaacaat acatagagga tgtagagcca ttgcattggc gcgatctttg
    24541 ccatgctcgg tccccagtta ttgggcagaa acgccacgca ggaggtgccc acgccagcca
    24601 atacggtgac cgcagccgtg agcgccataa acaaaatgcc gatgaatctt aatgtttttg
    24661 aaatgccact ttcaggtttc ataggaattc cttttcaaaa aaagattaga atgccagcaa
    24721 caccatggat gccagcatat ataccgaagc gtatttgaac aaatgaaaat tgagcctgga
    24781 gtttggttta aagagcatta aaacggccat caccagtagc cctccagaaa ggatgcccag
    24841 cacctgcagc gcgccctgct tcactccaac cattactgcc gcggtgccga ttaacccagc
    24901 ggcgaccagg ctggcaaggg cgacgatgcg gcgggttact ttttctccgt aggtggaagg
    24961 gaaagttgga atgcgcgctt tgtcgtaatc ctgctggtag cgcatactga aggtgaggat
    25021 gtgagttgga atccagaaca gtatggccag cgccaacagg atgccaatca aatctacttg
    25081 acccatgccc aacgcccgtc cagccaggat gggcatgccc ccggcgatac caccccaaac
    25141 gaccgaccaa caggtgcggc gttttaacag gatggtgtaa acgataacgt caaaaaagat
    25201 gccggcgaat attaacagcc catacaaagg cgaaagcgct gcagccagta tgactcccgc
    25261 ggcagaaaga cccagcccaa gccacagggc ttctgacgga gagatcgtcc cactggccaa
    25321 tgggcggtga tgggtgcggt tcatcacgct gtcaatgtcg cggtcatacc acatgttaag
    25381 tacggtgctg ccgctgatag agagcagcag gctggcaatc aaccctgtca tcgttgtcca
    25441 atgggtaaat ggacaacggg cgctcatata gcctgcgatg ccggtcactg taagcagcgc
    25501 ggtttgcagg cttttaatga gcgaccagta atggttgaat ttatgcagca atttcatcaa
    25561 tctttactcc acatcgcgca cacaacgaaa accggttccc gggctggtga atgttggcgt
    25621 ggcattgttc attgaccaga cgcgcaggtt gttttcgtac atatccttac tgccgccgcg
    25681 catgtagcgg taatgctgag cgtcatacac attggctgtc cactgccaga cgttgccggc
    25741 catatcgtaa acgccatagg ggctggcgga atccagcgtg gtgtagtgat tatacgtctt
    25801 cccgttgtaa aaaccaacag gagttgtgcg ggaaccgaag gtagccatgt tttcaaaagg
    25861 atcgcggctg aagtagaagt tcgcgttgtt gcgcgcaatt tcttcgcccc agggataagc
    25921 ccggccatct gtgccgcgcg ccgccttttc ccattccagc tcggtgggca agcgccagcc
    25981 attgaactca caataagatt tggcgccaaa ccacgaaacc atagtcatcg ggtggttttc
    26041 ccatcctggt ttcacagtga aactgtttcc gtcataagag aggcgcagga aagggtcatc
    26101 cagggggatg tgaatgtaac tgcctgcctt gatttcttcc tcatgtcgcc cgccagcgaa
    26161 agcatctcca ggatagtaac cttccacctg gttgccattg acctgaatat agtcagcagc
    26221 gagtgcttcg tttaagtaag cagcatattg agcgttggtc accggggtaa ccataatctc
    26281 gtacgcgtga tcgatcgtgg tttccttttt gaattgtcct tgcaaaaact cacctgctgg
    26341 tatctccacc cattgctcgc tgtccattcc agtatcgaac accggtggcg cagcctgaaa
    26401 atctacaggc tggcaggcgg tgagggcgaa aagcaggaaa agggacatgg tgatgatcga
    26461 ttttttcatt attgcacctc ctcctttttg gccaatcggt gggtcgtttc ttcttgccac
    26521 ctgcctttct ttttaaacag gtcagttagc cgccacacca tcaaggcggc cagcgccagc
    26581 aataacacga ttacgccaat atccccggct aacacagcgc ggttgaccag cggctgttgc
    26641 tcaccttcgg tgatgaaaag gcgcttcatc tcgtaaacgg tgacggcggc aaaggccacg
    26701 tctgaaaaga taatgaccgc ccaaccgaac cactttcgcg ctttcccctt caaccccgcc
    26761 aggtctgcgt aatagagaag caggatggtg gcggtaaggg tggcgaggat atgccagtga
    26821 ccggtcagga tcgagcgctc gtccataaat tgccatttgc ggaaaatttc atccagcttc
    26881 accgcggtga aaatacccac gccgctgacg gtgaagttca tgaacaccat ttgccaggta
    26941 ggaccaaaac gcaccgggtc tctcagcagc gcagcgacgc cctggaagaa gttgcctttg
    27001 cggatgcctt tttcatccag cccgctgcgg atgatctttt tccacgaatc gatcaccagg
    27061 aagagcgccc cgagcatggc cacggttgaa ccgccataga tgatgaaatg tgcgatttct
    27121 tcgaactgaa ccaccagcca ggtgccaagt gagagcacga aagcgcccac gatataaagc
    27181 ggcatggcca gtttctgcca cattcctttg aaatccatcc acctgccgat gatgagcgcg
    27241 cacatgatgg cgatgagcgc cagcatgata tgcaggtgac cgatgatgga aaggtccagc
    27301 gcggttttat gaggatcgcg caccacattt tcggccagga aggtttcaaa accgtttcca
    27361 tagaaagacc caggcacagc gccaaaaatc gccgaaatca aggtagtgag cgagaccatg
    27421 aagaaggcaa tacgctccat atcgtaacct ttttttgttt gagcgtagtc tttatccttc
    27481 aggcggtatt cgtctctcca gggccacaac gccaccagca gcaagcatcc cgcaaagaaa
    27541 ataagcgaca gaccggcgat gtagatgccg tgcagcaccc agttatgccc ccaataagca
    27601 aagggcagcc cgccaaacat caccatcagg tagccgacgg taaccgtggt gttgattggc
    27661 agacggtggt tgtctttcat tttcaataaa tcagtgatca tgtaaacttc cacggcgatg
    27721 gcgatcattg ccagcgagtg gtagaggatc accaatctgc cttcgcgctc ggcaggttcc
    27781 aggttgatgt tcaacgcgcg caccacaaca tccttgatgc cgtaatcagc catcgggcct
    27841 gagagcaagc caaagaatgc ggagccgaga gcggcgaacg cgattgccac taagatcaac
    27901 cctttggtgg agcggaagag gaactgatat cgtttattga gccattgcat tttacttctc
    27961 ccgtgtttgt gattaaccac tacacaatat aacatacccg gcggggataa gtcattgaca
    28021 ttaatcaaaa agacttatga caaacagcgg cgccgcgatg cttataaatc gatttttgcc
    28081 agttcctgca ggtgcctggg gtccagaatt tcaatttccg ttcggtttgc tcgcagcacc
    28141 ccggcggatt tgaaaacttt caacgtgcgg cttactgctt caggagaagt gataatacgg
    28201 gcggcgattg tgtgaatggg gttttccatg cggttgatgg atcgtcgacc gttctcgctg
    28261 agagaaatca agtgcttggc catgcgcgcc tgaactgtac ggaacgaaag gtcgtcataa
    28321 tgtgtgagca acaggcggtt gcgccgtgcc aacacgccaa gcaaactgat gccaatttgc
    28381 gggtattgcc tcagcaattg ttgaaaaggg agataaccga tacgccataa gcggatatcg
    28441 gtgtgcgcgt atgcgcttac cgggttcggg cctccgtcaa gagcggatac ttcattgaac
    28501 atgatgacgg gctcgagcgt gttgagaatg ccttcttttc cctctgggcc aagcttgctc
    28561 aaattgacct gtccattgag aagaacgaac attcccgcgg actcatcgcc ttcgtggaaa
    28621 acgaatgcgc ctttcacata ctgccgtatt tctccgctgg cgatgatctg ttctacaccg
    28681 ctcaacggta tttcgctgaa atgggtaatt ttctttaacc gctctgccag ctcgcgtgtt
    28741 tcactcatag gtcaattata tacattgtgc ggataaaaat tggtttttat ggtttaatgc
    28801 agtgatgaca tctttcacat ttgaaaaatt cattgccagc taccccgggt acaccgatac
    28861 ttcatacctc gaccggctgc gtgaactcga ttataaacgc ctggaacgcg aaaaacaagt
    28921 ttacctggat tacaccggtg gcgggttata cgcggattca caggtgataa agcaccaacg
    28981 catgctcctg gaaggcgttt atggcaaccc gcattcttca aaccccacat ctagcacctc
    29041 aacagccctg gtggaggata ctcgcgcgag ggtactgcgt tttttcaacg cctcgcctga
    29101 agattacgtg gtgattttta cggcgaacgc cagcggggct ttaaaactgg taggagaagc
    29161 ctatcccttc gggcaggatt gttgttacct gctgactttc gataaccaca attcagtgaa
    29221 cggtatccga gaatacgcgc tcgctaaagg agcgcacgtg cgctatctcc cggtgctgcc
    29281 gccgcgtttg cgcgcggttg agggcaagct ggatgaatac ctggcgaacg cgcgcggctc
    29341 atgccacaaa ctcttcgctt ttcccgccca atccaatttt tcttccgttc aacacccgtt
    29401 ggagtggatt gacaaagcgc atgccgcagg ctgggatgtg ctgctggacg cggcggcctt
    29461 tgctccgacc aacaggcttg atctctccag gtggcaggct gattttgttc ctatttcctt
    29521 ttataaaatg tttggttatc caactggtgt gggcgcgctc atcgcgcgcc gcgcagcgct
    29581 gaagaaactt cggcgcccat ggttcgccgg gggtaccatc actgtggcgt cggtgcaggg
    29641 agaaaagcat tacctggcag acgcccctgc ggcgtttgaa gatgggacgc tggattatct
    29701 gaacatcccg gctgtaaaaa ttgggctgga gcaccttgaa tccattggca tggaattgat
    29761 ccatgaacgg gtgaaatgcc tgacaggttg gttgctgacc tgcatgaaag acctgcgcca
    29821 tcgtaatgga aaaccgctgg taaaaatcta cggcccttcg gataccaagc agcgcggtgg
    29881 cgcggtgacg gtcaacttct tcaacccgga ggggatggtc attgaccacc gctggatcga
    29941 ggagaaagcc aacgcccgca atatctccct gcgcaccggc tgtttttgca accctggtgc
    30001 cggcgagctc gctctgcata tctcctctga agaattatct tcctgcttta atcgtcctga
    30061 tcatgaacaa cgcctgactt acgatgaatt tcggctttgc ctcaacacca aggcttcagg
    30121 agcggtgcgc atttctgtgg ggttggtaag caatttcgcg gatgggcagc ggctgcttga
    30181 ttttctcgcc acgctggttg aatgacggat cgtatggccg ccctttctca ttttctttac
    30241 cgtttttccc ggggatgggt ggtgttgctc gggtttattg tgttcatcgg gtttagcacg
    30301 ctcaccctgc cagggcaaaa tgccattgta gatgaatact cacagggcag cggctcgcct
    30361 gacacctcgc ttttctacaa cgcgaaacaa ctctatcaca tggctgaagt gtatgggcag
    30421 gagggaagaa cagcgtatct gaatgcccgc tggacttttg atgtggcttt cccgttcatc
    30481 tacaccttct tcctggtctc cgctatcggc tggttgctca atcaatcgct gccagtgggt
    30541 tcacgttggc gattaatcaa cctcgttcca ttggcagccc tggggctgga ttttctggag
    30601 aatacagcca ccagtgtggt catggcgcgt tatccacatc actgctcatt ggctgagtgg
    30661 ctggcgccag ttttcacgtt gcttaaatgg ctgcttgtcg gtggaagcct ggttgtcttg
    30721 cttgttgcca tctttttggt ccttctcagg cgaataaaaa agaacgcatg accagccgct
    30781 ggctggattt ataaaaatga ggtttttttc ctgtgtaaat agccgggcta ggggcgtttg
    30841 ctcgcggaat ggatggccat tgttcccagc atgcgcaccc ggaagttgac gcccgtgaac
    30901 ccgcagcgcc gcagactatc cgccaggtct tcggcgctga tgaaattttc gctggattgc
    30961 gtcaggtagg tataagcttc gctattgccg gtgaacagcc tgccaaggtt gggaataaca
    31021 tagcgaaaat agaacttgat cagcgggttt aacggcttcc agcgcggacg ggtggtatcc
    31081 agcaccacaa gcctgccacc gcttttgagc acccggtatt gttctgccag ggcagcatcc
    31141 agatctacca cgttgcgcac caggtaacca ctcaccacaa catcaaacgt gttgtgagag
    31201 aatggcagcg ccagggcgtc tgcctggcaa cggtggatat ttttccagtc ttttccggca
    31261 gccatcatct ccaggctgaa atcaacggcg ttgatacctg cgcccgggca ggtgtggcgc
    31321 gcttcgcgtg acaggtctcc ggtccctgcg ccgatatcca acaacgtttc gccgtttctc
    31381 agtttggcga tgcgcatcac ttccttgcgc agaaggatat ccacgccgaa ggtcatcaag
    31441 tggttcatca tattgtagcg gcgtgcaacg gacttaaaag tcttttttac ataccgttct
    31501 cgttcagttc caagcaggtg cgccattcgc ctattataaa tggaattatt tttccttttc
    31561 ctctgccagt ggaggctgtt gtgtgccttt ctgtggttcg agaagaatga atgccatgtg
    31621 tcccggtttt ccgggagaat cacttggttt tacttccctt tttgtagcac cctgttagga
    31681 tagggggatt cgaatacagc ttggctgggt tgaatactcg aggattttga tgcccgagaa
    31741 agggagagga gcggaaaaga atcagtgccc ccaagggaat tcgaatccct gttttggcct
    31801 tgagagggcc acgtcctggg ccgctagacg atgggggcaa gtgcaaggat tttatcacgg
    31861 cgaaatccca gtgtcaacga aatctacgct gagccgcaac gccctgacca tcaatttacc
    31921 gcaatcaatt gacgaaagcg gtcgggcatg ctattatgct tttacattat ttttattatt
    31981 tagttttatt ctggacatta acatttattc attcattttt attttgcagg agtaattatc
    32041 atgtcggtcg attttatttt tcgtattctt gggatggtca tcctggcgat tgtgggggga
    32101 attctgggta accagtttgg agaaattgcc aatacatctc caagcgccac cacttttccg
    32161 gtggagcagt atcgctttct gtttggattg ctgggagcgt tgttcgggct catcctcacg
    32221 ccatatttca ccacccgccc agcgcgggcg atgcgcaacg cgctggcaaa agccaccact
    32281 catgctctgg tggcctcttt atttgggttg gtggcgggtt taattgtggc cgccttgctg
    32341 gccttccctc tttctctgct gcccgatcca tttggcagcg tgctgccttt tgtgggcgca
    32401 atccttttta cctacttagg cgtggttatt tttaagctgc gacaacagga ttttctcaat
    32461 ttgctctcca acctgcgtag cggttcagga gtcacggcga aagatggggc atcaggcgcg
    32521 ggctgggcgg aaagccgcac catcttactg gataccagcg tgctcatcga tggtcgcatc
    32581 gcggacatct cgcgcacggg gtttctgcca ggctcgctgc ttatcccgcg ctttgtgctc
    32641 aacgaattgc aatacatcgc ggattctcct gataatatgc gccgtcaacg cggccgccgc
    32701 ggtatggagg tgctttccgc gctgcaaaaa gaacccgcca tccctgtgcg tatcagcgat
    32761 atcgatgtgg aaggaatgcg cgaggtggat gataaactgg tggtgctggc gcggcaaatg
    32821 cgctgcccga tccttaccaa cgactacaac cttaaccgta tcgctgaatt gcagggggtc
    32881 acggtgttga acatcaacga actggcgaac gcggtaaaat cagtgctcat ccccggtgaa
    32941 atcatgaccg tacaggtcat tcaagaaggg cgcgagaccg ggcagggtgt ggcgtacatg
    33001 gatgacggca ccatggttgt ggtagaaaat ggccgtgaat acatggataa atccatccag
    33061 gtgacggtca ccaaggtatt acagacggct gccgggcgga tgatctttgc ccggcccgat
    33121 gaagcagcga attagacccc gaacaggatt tcatatgaaa gtttacaaca cactatcccg
    33181 caaggttgag gaattccaga ccctcgaacc cggcaaggtg aaaatgtacg tctgcggccc
    33241 caccgtgtat gccaaggcgc atatcgggca tgccatgtct gcgctggtct tcgacatcat
    33301 tcgccgttat ctcgaatatc gaggatatga agtacgccac gtgatgaatt tcaccgacgt
    33361 agatgacaag atcatcctgc gcgcccaaga aaagggcgtg gacccattca aactgggcga
    33421 gatgtatatt catgaatttg aaaaacactt acaggatttc aacattttgc cggctaccgt
    33481 gaacccccgc gccacacggg aaatggagca gatcatcacc atgatccagg gattggtgga
    33541 gaaggggttt gcctatgaca aagatggcga tgtgtatttc agggtcacca gcgacagcca
    33601 gtatggcaaa ctctccggtc ggcgcattga tgacatgcag tcaggttacc gtatagaggt
    33661 ggatgaacgc aaagagcacc ccatggactt tgcgttgtgg aaggcagcca aacccggaga
    33721 gcccgcctgg gacagcccct ggggcaaagg tcgccccgga tggcatatcg agtgttctgc
    33781 catgaacctc aatcaccttg gcgaacagat tgatattcat ggagggggca atgacctggt
    33841 cttcccgcat catgagaatg aaatcgccca gagcgaaagt tataccggca aaacgttcgc
    33901 ccgttactgg attcacaatg gtatgctgca actgagcggc gagaagatgt ctaaatcggt
    33961 tggcaacctg gtaaccattg aagaattcct tgagaatcac ccggcagacg cgctgcgcat
    34021 gctggtgctg aattccggct accgcaatcc attgtctttc tctgatgaga tactcgcgca
    34081 ggcagagaaa gccattgaac ggctccgttc tggtctcaaa cccgcgcctg cggcagcgcc
    34141 cggggctgct caatcagtag tggacgccct ggcgcgccag gtggaaacgg cagaaagcgg
    34201 ctttgtgcaa tccatggatg atgatttcaa ttccgctggg gcgctggcgc aactttttga
    34261 cctggtgagg ggcatcaatc aggcgcgcgc ggatggcgcc actcacgcgc agctcagcgc
    34321 cgctcagctc aaattgcgcc agcttaccgc cgtgttaggg ctgagcctgg aaaaagagac
    34381 cgaaacccac caggaagtcg atggttttgt gaatttactc attgaaatcc gccgcgaatt
    34441 gcgcgcgaac aaactatggg aactctcaga cagcgtgcgc gaccggctgg ccgcgctggg
    34501 ggtggtgttg gaggacagta aggaaggcac ctcctggcat tggaaatgac cggcaaatga
    34561 aagaatggat tacggggcgc aacccggtta tggaagtgat gcaggcgaaa cgccgtcagg
    34621 tgtttcgcct gttgctggct tctggggtgg aagaaaaagg gcgcattgct gaaatactgc
    34681 gcgcagccgc ttcaataaaa gctccagtcg aacgcgtcgc gcgagaacgg ctggagcggc
    34741 ttggtgaaaa cccgcagggg gttgctgctg aggttggcgt ctacccttat gttgagttat
    34801 atgacattta tgacctggcg aaagaaagaa acgaagcgct gttcgtgctg gtgctggatg
    34861 tcattcagaa ccctcagaat ttaggcacat tgctgcgcac cgctgaagca gtgggagtgc
    34921 acggagttat catcccgcag caccgtgccg cagaggtaac gccggcagtg gtttccgctt
    34981 cggctggcgc ttcagagcac ttgctcatca cgcggagcaa tatcgcccgc agcctcgagc
    35041 agctcaaaga agccggcgcc tgggtggttg gcctggcgga aggggaggac gcgccagccg
    35101 aagtacccct gggcgggccc attgcgctag tggtggggaa tgaaggagag ggcatgcgcg
    35161 cgttggtcag gcaacagtgt gactatatcc tcagcctgcc aatgcgtgga cgcatccaat
    35221 cgcttaacgc cgctgttgct ggctcgatcg tgctctacca ggtgttgctt gcgcgccaga
    35281 aaacctcacg ctgaccgtgt tgttccacgt acggatgttc gttcatcacc tgctggtggt
    35341 ataggataac cgcgcaagta cctgggaagc cgccagggcg gccgtgttgg cgggttggtt
    35401 tctgaccgtc tttgatattc ctggaaagaa gcggtgggcg atgtatggtt tcgccaagca
    35461 gttccaacgt cctctctgtt ggggttttgc agcgggcatc cttactggtc acggctgtat
    35521 taaataaacg aggacagcgc catccagctg ctcccgttcc tgaaagatgt gcaagtactt
    35581 ttttaaagcc agagcaccca gaccacccac aagccaatca tcaggacgcc gattcccaaa
    35641 cgggcgatgg ttgcgaaacc gcaccctctg atgatcgccc cggctgattt tgcgccttcc
    35701 ttaagatctc gtttacgtac ccattctaca accagtatgc tcagcgcggc aaaaagcagc
    35761 ccgccaaagg gcggcaaaat aaaggtgccg gcgatcccag cggcgagcgc taccaccacc
    35821 gatatccaac tggcgccggt cttcttggct tcagcgccca tgaataagtt gtcaatgagg
    35881 ttgcccccta tcatcagcag cgtgatgacc gcaaacaaaa tgccggtggc aggtgaaaaa
    35941 ccgctgacga tgccgtaggc aaggatggcc agccagatga tagtgagccc agggatgata
    36001 tagagcatca gaccgaacag accaacgccc atgaccaccg cgagcacggg cacaaaccag
    36061 ttggaattcg caatggcatc caattttaag aaatcacttt caacccgccc atccagggca
    36121 gcagcacttc aggcacttta atttttccat cagcctgttg gttattttcg aacaccgcga
    36181 tgagggtccg cggcaggccc agtcctgacc cattaagggt gtgcaccagg cggttacgcc
    36241 ctccttcagc aggtttgtac ttgatgttgg cgcggcgcgc ctgaaaatcg ccgacattgg
    36301 aaacagaaga tacttccagc cactcgttgc aaccggcagc ccacacttca aggtcgtaag
    36361 tgaccacaga attaaagccc aggtcgccgg tgcataattg tttaacccgg tacggcaacc
    36421 ccagcaacgc acaggtttct tcggcgtcgg cgagcatttt ctgcagctcg tgcattgatt
    36481 cttcaggagt gctataaatg tacatctcaa ctttatcaaa ctggtgcccg cgtttgatgc
    36541 cgcgcacatc ccgcccggcg ctcatcttct cgcgcctgaa gcatggcgtg taagcggtaa
    36601 agcgcagcgg caggctggcc tcctccagca cctcatcgcg gtacataccg gtgaggggca
    36661 cctccgcggt tggcaccatc cacaggtctt cttcgatgtc gtgatacagg ttgtctacga
    36721 acttgggcag ctgcccggcg gcgtagaggg tttcagcttt gaccatgaaa ggtgtatagc
    36781 gttccacgta gccctggcgg gcgtgcagat caagcatcca tgagatgagc gcgcgctgca
    36841 ggcgtgcagc ctctccgttg agcacataga atcgcgaccc ggtcagcttt accccgcggt
    36901 caaagtcgat caaccccatc ttcggcccca actcccagtg aggcaggggg gtaaagtcaa
    36961 acttgggcag cgcaccgtgg gtacgcagta cgatgttctc gctctcgtct tttccaatag
    37021 gggtggaagg gtcaggaagg ttgggtatgg ttgccagcag cgcttgcagt ccttcatcca
    37081 cctgcctggt ggtctcatca agttgcgcga tttcctcgcc caccgaacgc atggcgtcga
    37141 tcttctcctg cctttcagcg gggtctttgg tctgactgat ttccttggag accgtgttgc
    37201 gcagggcttt gaggttttcc actttaacca gcagtccgcg cctttcagcg tccatggtta
    37261 ataacttgtc gaccacgctg ctgtccatct gccggtcggt gagcgctttg cgcagctcct
    37321 caggatgttc ccgtatgaaa ttgatgtcta acatgctgca cctcattttt aaagaataac
    37381 gccctcatct gtcgcgcagg acgaggggcg cctcgtggtg ccacctgctt tcgctcccgt
    37441 aggggaacct cattgtggta ttaacggtgc ctccggtctc tttacggcga gaatgcccct
    37501 gcagagacaa cctgcccaag aagggcgatc gggttggaat ccgcctgggc gttcctgccg
    37561 ctttgcagcg gtcagcggct ctctggaagg atatcccagg cagtagtacc cgagctggtc
    37621 ttgtaaagtg attatacctt gcgcttaatg cctgtcaaga acaacgcgcg caaggggatg
    37681 acagccgcgg tcaatcgtca gttaaacagg atgatggaaa aggacaaatt gcgataaaat
    37741 caattgatca tggacgaaca ggctttggtc gcagacgcgc tgcaaggaaa tgtagacgct
    37801 ttcaaccgtc tggtgctggc ataccaggac atggctttta accttgcttt ccgcatgctg
    37861 aacgatgatg acctggcggc cgacgcggtg caaaacgctt ttctttctgc ttaccgcaac
    37921 ctgcgcgctt accgcggagg ttcgttcaaa gcctgggtga tgcgcatggt taccaatggt
    37981 tgttacgacg aactgcgccg ccagaaacgc cgacccacga ttccgcttga accgcagaac
    38041 ccagaagatg gcgaggaaat tgaatctgcc gattggctgg cttctgatga cccccaaccc
    38101 gaacaggctt ttgaccaggc ggagcttgaa cacgccctgc agcactgcct ggaaaacctg
    38161 ccgcctgact ttcgcgcggt ggtcgttttg actgatgtgc aggggatgga ttatgaagaa
    38221 gtctcacggg cggtgcgcac gccgcttgga acggtgaaaa gccggctggc gcgcgccagg
    38281 cagagactgc gtgattgcct gcggcaattt caggaacttc tgcctactca atttcgtctt
    38341 accggtggag aacgccgatg actgagagat tgaccagcag agactggata ctgatttcag
    38401 cctaccttga cgggcgcctt tcacccgcag ccttaacccg catagaaaag cgcattcagg
    38461 aggacgctca ttttaagcga tctctcgaag aaatcgcgcg attgaagcaa ttactggctg
    38521 cgctgcccaa aaaacgcgcg ccgcgaaatt tcacccttgc gcctgaaaaa gcgcaaaaac
    38581 cgctgcgcgg attgtggctt caaccagcgt tgagttttgt gtctgtcacc gcgtcaattc
    38641 tcctggtgct ggtcttttcc tttaattact taggtatggg cttgccccgc caggcggctg
    38701 cccagcgggc ggaaatcgcc gccatggaag gggttgctga ggctcctgcg ccaatgatca
    38761 tcaattggat cccgtcaggc ggacagggcg gaggagcagc agaagccaag agcccctata
    38821 cagggatcga tggtatcgga gggccgggtt ttcctgttgc tgaaccgggt ataggcggcg
    38881 gaccagttga accggctgtt cctgaattac ctcccggcgt tgacccacag cccgtgatga
    38941 acgaggagcc tgagccgcct gcttatgatg aacccgcgcc cgccgcactg gagggcgaac
    39001 cggtttcaga cgctgacctt tctaacctga tacttggtct cgcggaagac gacacagccg
    39061 gtcaggtgat cgaaagcgcg ccgctgcgca aagcaccggc gccgcaacct cgccgcgggt
    39121 tttccgcgcc cagcctggtt atgttgatcg cgggcattac cgctctcctt tcaggcgcag
    39181 gagcgctctt cctccgtaaa aaatttcatg catagccatt cgcacgaaca tttcaaattc
    39241 tgtccgcagt gcgccacacc gctgcgcatg atgatgcagc tcggagagcg ccgaccttcc
    39301 tgccccgcgt gcggttgggt gcactacgag gatcccaaag tcgcggcggg tgtgctgctg
    39361 ctgcgcgggc gggaagtgct cctcgtgcgg cgcgttatgg agccttacct gggtacctgg
    39421 agcattccgg cagggtttgt caacgcattt gaagaccccg cggcggctgc cttgcgcgag
    39481 tgccgggaag aaaccgggtt gactgttgag atcgaagggt tgttcgacct gttgaccggg
    39541 agagaacaca gccgcggcgc ggatattttc atcgtttacc gcgcgcactt tctcagcggc
    39601 tctctcgccg ccgctgatga cgctgatcag gttggctggt tccctctgtt tgacctgccc
    39661 cctttggctt ttgaaaccac ccggcgagtc ctggaaaaag cccgccacct ccagctctga
    39721 gttacctcct gctggcgaga acaggcgcgt tcccaactct tgctcattcg aataatcacc
    39781 agttggcttg acctttcgga gggggaagtg atggttcgcg cgcagcacat ctatcttcgc
    39841 cactgatggg cgcgcaacag gtttttatcc atcgggatta cccgtcgaac aagggactgg
    39901 gctgaatgct aagatggtat gaaacctgca tccgtttgat gagaatggaa ataacgaatg
    39961 gccaaaagca cgaaaactcg aaaatcttac gctcatccgc ctaagtcctc aaggtcttcc
    40021 tccagaagag ggcagtattc gcgttcgacc ctgaccctgg accagcgcct gaacttcatc
    40081 ggcggggtga ccgttttgac cggggtactt tcgatcgtcg ccttattttc caatgccaat
    40141 ggtccgctca gtgggtggat cgcgcaaacc accacccgcc tcgctggctg gggtggcgtg
    40201 atcatcccgc tggcattgat cattggcggc acggtcctgc tgctgcgcca ttatgagaat
    40261 ctcccacggg tttcttactg gcgcctggcg ggcatcattt tgctttacct taatatcttg
    40321 ggctggtttc atttcatagc cggcgggggt ttttcgaccg cgaaagacgg gcggggtggc
    40381 gggtacatcg gcgctttgtt tgaccgcgtt ttagccaatt ctttgggccc ggctggagag
    40441 ggggtttttc tcttcgcctg gttactggtg gccattttgt tcatcctcaa cctcccgttg
    40501 gttgaaatcg ccgcccgtgt gaacacgctg ctggtcagat ttaagaaacc gaaagaagag
    40561 aacccgcgcc aacccatgtt ccacttcacc agcgccaggc cttaccgggt aaaaaatgaa
    40621 tcacgaacag acctgccgga tggttataaa ccgctcgtgg tggaggaaga aagcccggca
    40681 acggtgacgg tgatcgggag cccctcagcg gcggagccaa aaacaaccgg ggtcagagaa
    40741 aagcctgccc gggttgcgcc tgagccctct gaaagccttc ccgtcacccg cacacgggtg
    40801 accacgcccg tggcaccctg gccaattcca gcggtgagcg atatccttga cctggcgacc
    40861 ccagcggttg tgcaatctca ctacgaccaa ggccgcgccc gaattattga agaaacgctg
    40921 gcttcatttg acgcccccgg gcgcattgtt gaaattcagc gcgggcccgc ggttacccag
    40981 tatggcgtgg aacctgagtt cattgaaagc cgcggcgggc gcacgcgcgt gcgcgtgtcc
    41041 aagatcgccg ggctggcgaa tgacctggcg ctggctctct ccgccccgag cattcgcata
    41101 caggcgccgg tgccgggtaa agcctatatc ggcattgagg tacccaactc ggagctttca
    41161 ctggtttcgc tgcgcgagtt gattgagacg gacgcttata agaaaatcag gtccagcctg
    41221 cggtttgctt tcggcaagga tgtggcgggc aaacccattt gcgccgacct gacgtctatg
    41281 ccccacctgc tgatcgccgg caccaccaat tcaggcaaat ccgtgctggt caatgccatt
    41341 ctcaccagcc tgttgctcaa taacagcccg ctcgacctgc gcctggtgct ggtggacccc
    41401 aagcgggtgg agctgaccgg gtataacggc attccgcacc tgctcgcccc ggtggtggta
    41461 gacgcggaac gcgttgctgg cgcgctgcaa tgggtgctgg gcgaaatgga ttcgcgctac
    41521 aggcgctttg ctgaagaggg cgtgcgtaat atcatggatc acaaccgcaa agaggctgag
    41581 cgcctgccgt atattgtggt ggtgatcgat gagctggctg acctgatgct gctggcgaaa
    41641 gatgaaactg aacgcagcat tacccgcctg gcgcaactgg cgcgggccac aggcattcac
    41701 ctgatcatct ccacccagcg cccatcggtg gatgtgatca ccggggtgat caaggcgaac
    41761 ttgccggcgc gcgtagcctt catggtcgcc tccaacctgg acagccgcgt aatcatcgac
    41821 aagcccggcg cggaaacgct gctcggacgc ggcgatatgc tctatcaggc gccagacgcc
    41881 cccgcgcccg tgaggttgca gggtgtgtac gtttcagacc aggagattca acgcctggtt
    41941 aatgcgtggc gtctcatcgt ggccaatcgc cgcgctgaag gcgggaagga actctttacc
    42001 gccacctttg agtaccagcc ccagcacgtg ccgtttaaac aaccctcatt ttttggggat
    42061 ggcgcccagg aggatgaccc ggttttaaag aacgccatcg aaatcgtgcg caaagaaggt
    42121 aaagcctcca cctcttttct gcagcgccgt ttaggcattg gctatactcg cgcggcacgc
    42181 ctgatcgata ctctggaaga gcagaagatc atcgggccgc ggcaaatggg atcgcaggtt
    42241 cgcgaagtgt tggattttgg cgcggacaat gaagagcccc ccgctgaaac gcaatcggat
    42301 tgacccagaa agacagccgc tcattttccg ttctccccag agaataaaaa tagacaggtt
    42361 catttggccg gtttttcagt ataatatttt ttgaacactc cctgtgagcc agcaatacac
    42421 gcgaccgcgc cagccatgga aaggacgggt ggataatcac ataaccggtc gtgacaaacg
    42481 aggagaggat acccgatgcg caagcgcaat tctttttttc ttttaattgc cgttttattg
    42541 ctggggttct tggcatcttg ccgctccgcc aggctaccgt tgcccgcctt tttatcagcg
    42601 gatacgccca cgccaaccgc gaccatgacc ttcaccccca cgtacacccc cctgcccacg
    42661 aaaaccttca cccccacgcc cacgccgcca gtgaacttga tcccctgcgc caattctgat
    42721 gcctgttcag agggcgtata catccgtgaa tacctggaga cggtcggcct attggacgaa
    42781 gcagacgagg gagagcgacc gtttgaagtg gaggtgcctt acaaccttcc tctccgcttc
    42841 ggccttagct ggatcgccat cgatgatgag acgctggcgg ataacatgaa gaagatgacc
    42901 tttttctttg agattgatgg acagccttac ctggataaga acgatctgat ctacgaatac
    42961 taccctgatg tggaagaccc ttccatagtc tactcttcag tctttattga ttatttgttg
    43021 actggctgga ggatcggtga acctcatatg gtcagcattg gcttccgttt tgatcaatcg
    43081 ctttcagacg gctggaacac gtattacctc ggtgatacaa tcgggtacag ttaccgcatc
    43141 ctgcctgtgg agctgcccac cgccacgcct accccttccc ctactagcac accccagccg
    43201 cgacccaccg ccgtgccacc caccccaacg gtggcgtgcg agttgggcac cacgattgaa
    43261 atcaaaaatg acaccggcgg acaggtaacc atgcacttca ccggtccggt caagtacacc
    43321 ttttacatcg cgcccggctc gcgcacgctg cagctctgca ctggccaata cagctacacg
    43381 gcttacggct gcggcggatc ttcgacgagc ggcagcgcgg gcgatgggga tgaaatcgag
    43441 ttctggtgtg agtgacccga tcgcgctttt tgtgctgtcc gttggtgcga gcgagaccgc
    43501 gctcaggggt tggtgctgcg attcctgtgc cgaacggcgc gaacgatgag cagcaccgcc
    43561 agcacgataa agaacccagc caggaagaat gagcacatca gcaccgccag cgcctgcagc
    43621 gtgcgctctt gtacctgctg accagagcta tccacgcagt aggcgcgaaa ctgggtgctc
    43681 gcttcctcct cccaatctcc ctcctcctcc cactcctcga attgcatctc gctgccgggc
    43741 gggcaaacca cgccggtggt gagggcgaag agcgggcgcg tgaaaatcac cgccagcact
    43801 cccgcgggtg cgcagaaagc caccatggcg atgaacgccg atatgatcac cagtttcagc
    43861 aggctgctgc gcggtgcctg cacattcggc ggttgcaaag gatgattgtt catacttttc
    43921 attatgacac aaaaaggcgt ttttttaacc ctttgattgg cgcatcgtcc cggcggggat
    43981 tatggctcag gtttcccttt acttatcata gacaacccaa agaagaataa caatgacagc
    44041 cgaagagaaa gcaacaaggg acaaacaaca gagagtctcg tcaatgactc tctgttgttg
    44101 aaagaagatt gattgatcta caagcccgcc gcgcttctca ataacccggc tttgtctgtc
    44161 ctctcccagg tgaactcggg cagctcgcga ccgaagtgcc cgtaattgga ggtcttgcgg
    44221 tagatgggac ggcgcaggtc gaggtcgcgg atgatggcgc cggggcgcag gtcgaagtgc
    44281 tcgctcacca gcgcggcgat cttctcatcg gggatgacgc cggtgccgaa ggtctcaaca
    44341 ttaatcgaga gcgggcgcgc cacgccaatg gcgtaagcca actggatctc gcagcgcgtg
    44401 gcgagcccgg cggcgacgat gttcttggct gcccagcgcg cggcataagc ggcggagcgg
    44461 tccaccttgg tggggtcttt accgctgaaa gcgccgccac catggcggcc catgccgccg
    44521 taggaatcca cgatgatctt gcgcccggta acgccggcgt ctcccatggg tccgccgatg
    44581 acgaagcgcc cggtggggtt gacgtagatc ttgggcgtgc cgtccagcag ctccgcggga
    44641 atggcggggt tgatgacgtg ctcgatgacg ccctcggtga tatccgcgtg cgaaacatca
    44701 gcggtgtgct gggtggaaac gaccacggta tccacgcgca ctggcttgcc gtaagcgtac
    44761 tccacggtca cctgcgactt gccgtcaggg cgcagccagg gcaggaggcc ggctttgcgc
    44821 acctccgcca ggcgctgggt aaggcggtgc gccaggtaga tgggcatggg catgagggtg
    44881 ggggtttcat cgcaggcgta accgaacatc atcccctggt cgccggcgcc gatatgctcc
    44941 agctcggttt ctttgtcctc gccgcctttg gcttcgtatg atttattcac gccgagggcg
    45001 atatcctggc tctgcgaaga aatggcagag agcacgccgc aggtattacc gtcaaaacct
    45061 ttatcagaag aatcgtagcc gatgctgttg accacctggc gcaccaggtc gttgaagttg
    45121 acgaaagcgt tagtggtgat ctcgccgagg atcatcacgt agccggtctt gacggcggtc
    45181 tcgcaggcca cgcgcgagcg cgggtcttgc tcgaggcatg cgtcgagcac ggcgtctgag
    45241 acctggtcac agagtttatc ggggtgccct tccgtgaccg actcggaggt gaatttaaaa
    45301 ctggggtttt gcatgaaaga actgcctgac atgggttttt ctccttataa aaataagatg
    45361 ttgccccaga ccggtgaact cgaggggcaa catgagcgtt tggctgccct ctatcttcca
    45421 gattgtttcc ctgccggaat tggcaccatg tcgcgagtta cgctcctggt tgccgaggct
    45481 tcatcgggcc ggtccctccg cctctctgga tagaagtgcc gtttttaggg gctattcaat
    45541 taaggtcagt ttatcacggg gataggcggg tgtcaaatga caaaagacat attcttgcgt
    45601 atcaggataa ggcgcggtac gcccgctggg ggggttatcc cggcgtcgcg ctgggttctt
    45661 ccgtgggctc aggaggctcc tccgtcggct caggggactc cgctgtgggc tggctggggg
    45721 gcggcgtgtt cacctgggtg gtgctggtgg gtcgggcggt gggcggcgtc tgcggggttt
    45781 cagcgggggt ggagggcaga ggggtaagcg tgaccgtggg gtgaggagtg ggcgcgggcg
    45841 tgagcacggg cagggtgtgg gtatccccaa ggacgttgac aaaagcagcc ggcacccagc
    45901 aatcgggcgc gcgctttttg ggcaaacgca cataaaagaa agcgtcatca gggctgcggg
    45961 cgagcgcctg cagcacatca ccggtgcgca ggatgaggta actctcaaat gagctatcgg
    46021 gcccagagcg gcagttcaca cccagttcaa cttcgttgac cgtgagctgg acaatttgcg
    46081 agatgggcaa ggcggtgggg atttcaagat ggctgagcaa agaggtggcg gtggggctgg
    46141 gctgctcagg gcgcggcgaa ggcgtgcgcg tggggaagtc gtagggctga tagggtgttt
    46201 gcgtatcttc gggcacatac gcctggatga tgtgagagat gcgtgccggt gcgccgagga
    46261 ggaagaaccc ggccagcagc ccagcaccga agaccgccag cgcggacttg atgaaaatgg
    46321 cgcggaaaac cgccgggttt tgtacccagc ggaggctggc gggcaggcgc gcgggggcgg
    46381 cttgatgaag gatggggcaa tcgcgatagc gctgcgagag gcagtactcc tgttgctgct
    46441 ggcgtgaagg cacgcagacc gattggcaat gcaggcagtg gttgacctct gaagggaagg
    46501 cggtggtggt tgtggtgtca tcgagcatgc ccaggtgcgg gcaggtgtaa aggtcgtgtg
    46561 cggttttcgc ggaagaactg ctggagctgc tcataccctg accggcggcg attcccggtt
    46621 ttcccctctc tggcgcactg gcgaaacgcg cgcccaggat aaattaaaac gtgtccaatt
    46681 aaaagatgtg aatattgact tcgctgttaa taagatacca taaatcgtag tgcgtgtata
    46741 tcaggctaac cttaaaaaaa gcgaggagcg aagtgatctt tcagcgtttg gcagccgcgc
    46801 ctgcgcggac gcggtttgct caggcttctt tgcggatggt gtaagaatca aaatcgcggg
    46861 cgacgctggt gttttcgaat accgcctggg cctcagcgag gatatcttta tcgcggtagc
    46921 ggcggcttag atgagtgaga atgagcttgc cagcgccaat gctcgcggcg aactcgcccg
    46981 cctgacgggc ggtgaggtgg gcgtactggc gcgccatact ggcttcttct tccagatagg
    47041 tggcttccat taccagcccg tcaacgctgc gcagcacctc ggtgagcgaa gcggtttcgc
    47101 cgaggtcgcc gagggcggcc aggcgggcgc cgggtttaaa ggggccggtg accatgctgg
    47161 gggtgataac ccgcccgtcg agcagggtga cgctttctcc tttgaccagt ttgccgcgca
    47221 gcggcccggc gggcacgccc agcgcgtcgg cggcttccgc caggaagggc tggcgtccct
    47281 tctcctcgaa gagatagccg tagttatccg gtccgcggtg gttcacagcg aaggcgctga
    47341 tggtgaaaga aggagcttca aagatcacgc ccggggtgac cgggatcagc tccacaggca
    47401 ttggagccct ggcgccgcgc agcaccacgc cgtgcaccag gtcgctgatg cgttcgagcg
    47461 cgttagcgcc gccgtagatt tccaggcggt caatggtttc ccaacgcatg aaggtggaga
    47521 gcaatccgcc aagcccgagg atgtggtcga ggtgaccgtg ggtgatgagg atgcggttga
    47581 gacgcttgaa gccgatgccc gcctgcagga tctgccgctg ggtgccctct ccgcaatcga
    47641 ttaaaaagcg gtattcgtcg tgctgcacca ggtaggcggg aagcccgcgc cggggggagg
    47701 gagcagaggc ggaagtaccg aggaagatga tctcaaacaa tggcgcggtc ctttcaacag
    47761 gtggttttat tttaccctaa gttaagcggc ggtcgggaat ggcacgaagc ttgcaaggat
    47821 gagggcacgg agaaggattc gccgccgggt gtctgaacgg ctgttcagcc cgcgcgcaaa
    47881 acgaagcgag gtgaatatgg aattcttaag cgtgagcatc cacaaacccg aagcgctcaa
    47941 tttcattctc gggcaatctc attttattaa aaccgtggaa gacatccacg aggcgctggt
    48001 gacggcggtg ccgggcattc agtttgggct ggcgttttgc gaagcctccg gcgcctgcct
    48061 gatccgctca tccggcacca atgaagcgct gacgcagctg gcgcgcgaaa acgctatgaa
    48121 catcggcgcg gggcactgct tcattttgat gttgggtgaa ggttattttc ccatcaatgt
    48181 gttgaacctg gtcaagaaca ttcctgaggt gtgcgcgatt ttctgcgcga ccgccaatcc
    48241 cactgaggtg gtgatttacg ataacgggta tgggcgcggc atcatgggca ttgtggaagg
    48301 cgaaaagccc aaggggtttg agagcccgga agacgtgcgc tggcgtgtgg actttttacg
    48361 tcgaatagga tacaaggccg ggtaacgcgc aggcgccgcc tggaacacaa ggagccaaag
    48421 atgaaaaaat taattcctgt actgatcatt ctcagcctgg tgctgaccgc ctgtggcaac
    48481 ttaccgctgg gacgaacgga agaggacatg agcgatagcg agatggccac gcgtgtggcg
    48541 gaactgcttt caaccatgac cacccccact accgagatgg tctttccgcc cacgcccacg
    48601 ccgcaggccg ccaccgaggc tccgcctgag cccagcgcgg cacccgccac gcaggagccg
    48661 gcagcgctgc cggcgaccga gacccccgtg gtggtggagg aaaccctgct ccccgaggaa
    48721 gccggcacgc ccacaaccga ggtgacacca gcgctgccgg aaaccgaccc ggcgcgcaaa
    48781 ctgggcaacc ccacccacag cgacccgctg gacagctatg agaagtggac ctggccgaca
    48841 ggcagcgatg attacctcaa ggtaaacttt aaagacggct ttctggaaat gaccgggctg
    48901 accaccatgg cgggctggcg cctgccgatg ctggcgcagc agaccaacag ctacattgaa
    48961 cttaccgtca actcaggcgc ctgccaggga aaggattcgt acggcatcat tttccgcgtg
    49021 ccagtgttca aagaaccgac acagggctac ttgtatgagg tcacctgcga tggttacctg
    49081 cgcctgtggg aatggaatgg taaagccaaa cccaaaggcg aggctgacgt tctgatcaac
    49141 tggaaagagt cgatggatat taagaccggc gccaaccagg tgaaccgcct gggagtgatg
    49201 gcggtgggca aaagcctgcg catctatatg aatgggattc cgcagggcga ggtgagcgat
    49261 tcgacctacc cggcgggatt tgtcggcgct tttgtgcgct cggtgaccac ccccaagtac
    49321 acggtgaaat ttgacgaaat gaaggtctgg gaaaacccgg cgcagtaacg gtgtacggaa
    49381 ggaaatggag cgcggagccg ccaggggcgg ctccgcgttt tgtgttgcct agagattttt
    49441 tcgctgctgc gcgaaaacag cattcaggct aaaatcagac ggtaaggagt ggacgaaatg
    49501 aaaagttacc gcaaagagat cacgctcaac atccccgcgc ggcgggggtt tgtgaacatc
    49561 acgccgcagg tgcaggcctg cctgcgcgag agcggcatca gcgaagggct ggtgctggtg
    49621 aacccgatgc acattaccgc ttcggtgttc attaatgatg acgagagcgg tttgcaccgt
    49681 gattacgacg cctggctgga acgattggca ccgcatgaac cggtgaacca gtaccgccac
    49741 aacgacaccg gcgaagataa cgccgacgcg cacatgaaac gtcagctgat ggggcgcgag
    49801 gtggtagtgg cggtaaccgg tgggcgtctg gactttggca cctgggagca gatcttttac
    49861 ggcgagtttg atgggcggcg gcccaagcgc gtgctggtga agatcattgg tgagtgagat
    49921 gagcaaccgc gtttatttcg gcgaaaatct cgaggtgctg cgcgcgctgc ccgacgcatc
    49981 ggtggagctg atctacattg atcccccgtt caacaccggc aaggtgcagg cgcgcacgcg
    50041 gctgcgcacc acgcgcgcag agaatggcga ccgggtgggc tttcaggggc agcgctacca
    50101 gacggtacgg ctgggttcgc tgggctacaa tgacattttc gatgattttt tggctttcat
    50161 cgaaccgcgc ctgttggagg cgcaccgtgt gctcagcgcc gcgggcagcc tgtattttca
    50221 catcgattac cgcgaggcac attattgcaa ggtgctgttg gatagtattt ttgggcgcgc
    50281 atgttttttg aatgagatca tctgggcata cgattacggc gcgcgcacgc ggcgtaaatg
    50341 gcctcccaag cacgatacaa ttttttggta cgtcaaagac ccgctgcgtt acactttcaa
    50401 ttatgacgcc attgaacgca ttccctacat ggcgccaggc ctggttggac cggaaaaagc
    50461 cgcgcgcggc aaaatcccca cagatacctg gtggcatacc atcgtgccca ccaacagtgg
    50521 tgaacgcacc ggttacccca cgcaaaaacc gctgggcatt ctgcggcgca ttgtggctgc
    50581 ctcatccaac cccggcgaga cggtgctgga tttctttgct ggcagtggca ccaccggcgc
    50641 cgcctgcctg gaactggggc gcaggttcgt cctggtcgat aacaatccgc aggcgctggc
    50701 ggtgatgact gaacgcttta aaggcgtaac ggatatcgaa tggatcatcg acacgcctga
    50761 agccatggac cgatgaaaga atatcttctg gcgcgcccgc agcccggtta gcacgaaccg
    50821 gcagcggtgt tttcgcccac gcggcatgta aacacgggca cgcctacctg tgagccgcgc
    50881 agcctgcgcg accccgagct ggcggtggag acggccgggt ttatgtgggt ctcaagcagg
    50941 ttgaaacgaa gactacctga aggttgggtt gagggcgcgc acctgttccg cggcgtggta
    51001 agaagagcgc accagcgggc cgctttctac ccagccgaag ccgagagata acccgtattc
    51061 cttaagatca gcgaattctt ccagtgtgta ataacgttcg atgggcaggt ggtgccggct
    51121 gggttgcagg tactgcccga gggtcagaat atccactccc cagtcgcgca ggtcgcgcat
    51181 gacggctttc aattccgaca tggtttcgcc caatccgacc atcaaaccgg atttggtcag
    51241 cacatccggg gcgagcagtt tggcgtggcg caaaacggct tctgaccact cgtagtggtc
    51301 ctgcggctgc actttcttga acaaagaggg tacggtttcc acgttgtggt tgagaatctc
    51361 agggcgggcg tccatgacca tttttaacgc cgggaggctg gctttgaaat cagggatgag
    51421 cacttcgact gaacagcctg gcaccagctc gtggatacga cgaatgaccg cggcaaagat
    51481 gggggcaccg ccgtcttcgc gctcatcccg gttgaccgag gtgatgacgg cgtgcttgag
    51541 gttcatcgct tttaccgcct gcgccacgcg ttcgggttct tcccaatcca gcggggcggg
    51601 gcgcccgtgc ttgacatcgc agaagcggca ggaacgcgtg caaatttctc ccagcatgag
    51661 aaaagtggcg gtaccgctgc cccagcattc tcccatgttc gggcatccgg cttcttcgca
    51721 aacggtatgc agagaggttc gacgcatcag cgcctgcaat tgagcgtagg tctcgcctgt
    51781 cggggctttc actttaatcc aatccgggcg gcggcgcggc ctggggtctg aaggagcggg
    51841 ctggatacgg tcacgggtgg gtgtgttggc catggtggtc tccagaacga attatacctc
    51901 gcatgaagtg tggtgctgat gataaaatgc attataagcg gtacggccgg aggaacgggg
    51961 tttgcgtgat atactgcccc tatcagtgaa tcaagcatgt tgatcttagt tgccacaccc
    52021 atcggtaacc tgggagatat ctcgcaacgc gcgttggcgg cgcttgaaaa cgcggacgtg
    52081 gtggtgagtg aagatacccg taagactggt ttcctcttaa accatttcgg cattcagaaa
    52141 cctcaaatct ctttcagaga agataatgag cagcgcgcgc tgccaaaggt gatggagttc
    52201 ctggaattgg ggaaaaccgt ggccctggtc accgacgccg gcacgccctc tatttctgac
    52261 cccgggttca tcctggtgcg cagcgcgatt gaaaaagcaa tcgaggtctc ggcggtgcct
    52321 ggtccaaccg cgcttattat ggcgttggtg ctgtccggtc tgccggtgca cagcttcact
    52381 ttcaggggat tcccgccgcg caagagcgct gccaggcagc gctggctgga aattgactgc
    52441 gactctccgc acacgctgat cttttatgaa agcccccacc gcctgctggc attcctgcaa
    52501 gacgcgcagg cggtgtacgg caatcgcagg tgcgcagtgg cgaacgacct gaccaaaaaa
    52561 ttcgagaccg tctaccgagg aaccctcaaa gaggtatcag agcagttagc gcgggaaaaa
    52621 ctgttgggcg agtatgttgt ggtgattgcc ggcgcggagg attaaccgtt ccagtcaaag
    52681 atgctgaacc tggtgtgcgt gtcgaagtaa tcttcctgtt cctgtctttt taacgtgctg
    52741 accacccaat acgtgaccgg cgtcatgagg acttcgatgg cggttttaaa aaggtaattg
    52801 gtcaccgtca gcgtccagaa aaggctccag gggaagacgc cggtgagagt ggcgatactg
    52861 acaaaaacca gcgaatccac tgcttcgccc accagggtgc tgccgatggt gcgcgcccac
    52921 aaccattttc ctctggtggc gaccttcatt tttgccagca caatggaatt catgaattct
    52981 cccgccaggt aggcgaggag gctggcgagc acgatcccgc cggaggtgag cccgcccagt
    53041 atggcgttat aagcgccctg accgctttgc tccacccagg tggcttcacc aggcatcacg
    53101 cgcactagcc agaacaccgc ggcggtgagc gccaggcagc cgaacccggt ccagatgaca
    53161 cggcgggagc gtttaaagcc gtacacttcg gttagaacat cgccgaaaat gtaactgatg
    53221 gggaaaagaa ttgtgccagc gtcaaaggca agcggtagat taaagatgga cactccccaa
    53281 tcgacaattt tcgccgatga agcgatgttg ctgatgatga gcacggtgac aaaaagcgcc
    53341 atcaccaggt caaaataacg gtaggaacgc tgaggggttt tatccatgct gattcagaaa
    53401 cacgaagggt aacctcatgc ctttagtata atccagtaca tacataagcg gagagaggaa
    53461 tgcagcgctt ttttgtgaac ccagaggaaa tccaggggca ggaagtggtt tttgcgcctg
    53521 cgcgctccag gcagatcgcg cgtgtattgc ggttgaaaaa tggccagaat tgcatggtgt
    53581 tggataaccg tgggagcgag ctgctggtgg aattgacgca ggtggaagcg ggtgcctgcc
    53641 gggcggtaat tgtagagcgg gctgaagcgc aacgggaacg gattgacctg ttgatgctgt
    53701 taagcctgac ccagcgcgag aaattcgaat ggatgctgca gaagtgcacc gaggtggggg
    53761 tttcgacgtt tcagccggtg atcaccagcc gttcgttggt gcagcaaccc gcacagacaa
    53821 ttgccaagta tgaacgctgg aagaccatca tttgggaagc cgcagaacaa agcgccagga
    53881 gccgcctgcc agaattgctg ccgcctgccg ggctggctga ggctgttgcg gaagcggctc
    53941 gacgttgcca gcgctgcctg atcccctggg agggcgagaa ggcacggaca ctcaaggggg
    54001 cattggctgg cccagcagtt ggcagcgttg cgctgttcat tggccccgaa ggcgggttcg
    54061 cggaaggaga agtacagcag gcggtaaatg ctagtttcaa cgtggtgaca ctgggcaggc
    54121 gtattttacg catggaaact gccgcggtgg tcagcgcggc gctggtattg catgaactgg
    54181 aatctacaga agcagatgac gggggaacag gttcttcagg gccgattggg tgactttccc
    54241 gcagcccagt gacagtccct cctggttcaa aataatacga aagcggtcag tttcttttgc
    54301 cgggctgcca cgtggtaaaa ggtccgcgcc agaaagccac aaccggcttt caatctcatc
    54361 gagttgcacg atgttccgct ggcaccgagc accaaaacgg ctggcccact ggtgagaagg
    54421 caagaaaccg ctggctgttt gctgcccgag cagcatcccg gcggattgca ggggtagagc
    54481 agggaacttc tccagcaaga gcatgggtaa taaatgcatt tcttcgaagt ggcgaaccaa
    54541 agtacgctga tgactgctga tatccgcgag caagtcataa gcgtaagtgt tatgaaattc
    54601 ctcgcaaaag gctcgctgct cacgggcggt taattcttca aaacccacct gggcgagcgg
    54661 acgagatggc ggtgaggttg ggcgcatcgg cgtatgctct attttacgca agtgacaggc
    54721 gaagaacccg gcagtgtgat agcggtgggg ccacaggcgg atggctttgc tcaaagcggg
    54781 gtgcagttcg gcaccatcca gcgaggtaat ggcaggcgcg ggcgcgggca atttggtttg
    54841 cgcgtcaagt atctccacgg cagaaccgaa gacgcgtagt aattcctcta ctaccatctc
    54901 atcttcctgc ggcaggagcg tgcaggtgga atagaccagt tctccgccca cgcgcagggt
    54961 gcgcagggcg ctggtaagca gggctaactg gcgacgagaa agctggcggc tttctttttc
    55021 tgtcacgggt cgggaaatgt gtgattctgc agttcgcaac ccctgcatgc tgcagggggc
    55081 atcaaggagc accttgtcga acatgctcgg gaaccactcg ccaaagcgct ctccagggaa
    55141 acgactgacc gccgcattcg ccgcacccca ttgttgcagc acaaggcgca gcgcctgaat
    55201 gcgcccctgg ctggagtcgt tggcgatcac caaccccgaa tcttccagcc ggctgatgag
    55261 atgcgtggtc tttccccccg gagaagctgc caggtcgagg accagctctt cacgctgcgg
    55321 ggaaaagttg aacaactcag gcggcagcat ggaggctgct tcctgaatgt acatcaagcc
    55381 caggcggtgt tcaagcacag cgctgacttc tggcccagcc ccgatggtaa cgcggtaacc
    55441 cgcggggcag aagggaacgg cttccaactg ccagccgtat ttttcactca ggcgcgatgc
    55501 caggttgggc ggggccttga gcgggttcaa acggatggct gtcggcaaag gctggttgat
    55561 ttcattcacc agcagcttaa attcttcctc cgtcagcagg ggtttgtaag cagccgccat
    55621 ttcaccgcta tcagcgggcg gaactgtggg tcgctggcgc ctgttaggtt tttggctcat
    55681 gcgtccgccc tgatgcaaaa gacgatttcg ctgtcaaaga gcagttcgct cacctgccaa
    55741 cctcgctgcg cgcaggcttc ggccaccagc cggcggccgc ttgttagcag cgaacgctgt
    55801 ccattgagcg aaacggtggg caaagagacc agcagccagc gcgttcgtag ccgatgaaaa
    55861 aactggcggt tcacccctcg ttctcgctgt tcaaatcggt gggcttcttt gaagaaaaag
    55921 accacatccg cctccatctc cgggggttct accaggatgt ctcggtgata ggcgctgcca
    55981 gtttgaccga tatgttggaa gaaggcgtcg atcaagtcaa cgcgaggctg atgcaggtcg
    56041 taggcgtaat aagctgtttc gcggggcagt cccatccagg ggagcgcgaa agggttcaaa
    56101 ccgcaggcca ggtccagcac cacgcgtgga gtgccggtga acctgaagat ctcatggtaa
    56161 aaaagcgata gcatgggcag cctctcgcgg gtggaggcgt gggctttcaa cagttgagcg
    56221 caccattcca ttacctcagc agcgtgcggt tgatttggca gagccgccag cacctggcgc
    56281 atcattgtgt aatcagggtc acccaggtac gtcgccaaca ggttgtgaag tttttggcga
    56341 aatacttttt ccacctggcg ggggtcggaa caggcgcgca aggttttttc aagcagatct
    56401 cgcagcgtag tatcaggtat tcccagcgcg cgatacttac gtgaagcgct gacgcgcgcg
    56461 agcagcgctg catattgatc ttcggtggga gaagcggaga ttttcttcat ggcgcgcggg
    56521 tcgtcacctc tggattaaaa acgcgagttc gcccgggaac tcaaagcgag taacctgcca
    56581 cgctttgccg gcgagtagct ctgcaaagcg ccgctcgtag ttgcgcacca tgcccttgga
    56641 ttttcctcct aggctgtggg cggggaatga aacgagaata ttgggagatg agatcatctc
    56701 cagaagacgc cgaccggcat ccttatcgat ctgttccagg caggggatgg tcttcagcac
    56761 cagagtgagg tcagccttga caggaggtaa ctcgcgggta atatcgcacg gtactgcatg
    56821 gccatcaatc ttaaacctgg cgaaaaaagc gttgagaaaa tcaagcatat ccgtgtagat
    56881 atcacaggcg gtgtaagtga agccaggctg cagcggcagc cagggaatga ccagcgggtt
    56941 taacccgcaa gcgaggtcca aaatggaatg gacgctgccg agaggagcca ggcactcctg
    57001 gaaaaaaacg gcctggatgg gtatccgttc gcgggtagac gcgtgagccg acaaagcgtg
    57061 cataaggtaa ctcagcacat ccgggtgatg gaggtcttgt ggaagctgcg cgagctcttt
    57121 taaccaggct gcataaggga taggttttcc ctggtaggcg gcggctacct ggtggagttt
    57181 actgcgcgcg gctttgactg cttctttggg gccgtgcctt ttttccattt cggcagtcag
    57241 cacggagcgt accaaaacct cagagagatg gcgatacttt tcagcccgct gcacctgctc
    57301 tacaagggcg tcaagcaggg tatccggctc agccatgcgc agcctgttcc cggatatgtt
    57361 cagtgagctg caccataaag cgcaggttgt gcaaggtgca caggcgcata aaaagcggat
    57421 caccctgttt aaagagatgg tagagatacc cgagcgggta gtcgcggcag caggggcaat
    57481 cacaccactg gctgatgggc tggctggagc ggatgctgcg cgggttatcc acgtactggt
    57541 aggcgaacca atctccggtg agccctggcg cgccgggcga tggcgcgctg gtgaaggtgt
    57601 acaacctgcc gtgacgggcg tcgcgggtgg gcatggcact gtcgaacagg tcgtaaccga
    57661 ggtgataagt ggcaagtaaa ttctgtggat gcccgatgcc aagggcgtgc atggggaaag
    57721 cggcggggat gagttggcga gtgtaagcga tgatctcggt gagcagccgg ttttcaccat
    57781 ctaaaggcca tccgccaaaa ccaaagccat cgaagcctat ctccagcaag gcatccgcgc
    57841 agcgtttgcg ttgctcttta aaaccgccgc cctgcaccac ggcgaagagc agcggtcgct
    57901 gctgccccgt ttgttctttt tgctctgcca gcctgagata cgctgccttg ctgagacgcg
    57961 cccaggcgat ggtgcgttcc accgaaaggc gctgggcagc ttcgtcttca tccacatgcg
    58021 tgcaatcatc caggcagaaa acaatatccg cgccgtagct catttgcaac tggatggcct
    58081 tttgcggggt gagctgaaat ttgcggctgg agccttcagg ataaaataaa atgcctttgt
    58141 cgttaatgct gccgtagcgg gcgttttgac ggatgagcga ataagcctgg aacccgcccg
    58201 agtcggtgat aatcggccgg tgccaaccgg tcatttggtg tagaccgccg agcgcctgga
    58261 tggtggaaga ccccggtttt tgggtgaggt gaaacgtgtt cattaccagc gcttcaatac
    58321 cgatacctgc caaatcacgg gcatccaccg tgcgcacaac gccgtaagtg gcatcgggca
    58381 ggaaaactgg aaagtgaacc ttaccgctga cgagttcgag gttggcggct ctcactgttt
    58441 actcgacctc aggcgtctgg tgttcggacg aggattttgt cttcacaaaa tgattataag
    58501 gtatgaacgc ggcaaatcag gcttttctcg tttcaggcac cttgagcaaa gcgcctgcgg
    58561 agaggaacat cagcagggcg gaaatcgcga acagcgcggt ccaaccccac cactgaccag
    58621 gagcagcgtt attgagggcg tcggtcatgg gtccttccaa cctggcgatg gccgcggaac
    58681 cagcggtggc gatgttggtg agacccatgt acttggcggc gttatccttg gggaccatgt
    58741 cgttggcaat cgcccagttc acactgagaa agatacccag cccgacaccc ataaaaccgc
    58801 cgtagatgag caattgggca ggcgttttgg cgaaaatgag cagaatggcg ccgataccgc
    58861 cgatgatgga tgaaaggatt tgtagttttt tacgaccgta acggtcgctg agggtgcccg
    58921 ccaacaggct gaagatgatg aggacaacga cgaaagtacc catgacgaac tgggtgaaca
    58981 tgatgggatc ctgctcaggg aacttatcgc ggatgaaata ctgggcgaag gattggaaac
    59041 cgtagatacc caccaggtag agaaaacggc tgaggatgag ctgccagtaa tgcttatctc
    59101 caacgacctt gaagttgaag gtttctttga tcgccctgcc cagctgcagg tggttcctgc
    59161 cttcaccgat ggagggactt tctttagtga aaaccaaggt gatgagacca aataccacca
    59221 gtaaggcgat gatgactgtg accgcgagcc gcgggtttgg gtcggaggag gagatgagaa
    59281 aacccatcaa cagggaggag agcacaatgc ctgccatatc cagtgcggtc ttgatgccgc
    59341 tggcaagccc gagcttttca gcaggaacct catccggcag caacccctga gcgggtccat
    59401 gagcgatatt ggaggtggtt tgcagaccga tgtagccgat gaacagcgcg ggcagaccgc
    59461 cgataaaacc catgagcagc aaaaaaaaca ggtctcctgc tgtgccaaac cacatcagcg
    59521 ggcggcgccg tccgagtttc gatcgccaat ggtcgctgac cgcgccagag acgggttgga
    59581 caagcatggc gatgatcaag ccgaagaagg tgagcagccc aagccaggtg ttcttctggg
    59641 tatctggcac gtatttaagg agcacggctg gcaggatggt aacgtgcagg ctgttccaca
    59701 tgaaggaaag accaatccaa taggcgttga gcacaaaaag ataccagcca ttcactcgtt
    59761 tcatcgtttt ctcctgatag aagggcatct gtttatcggg ggcttactcc ggcatggtct
    59821 tgaacacctt gtcccacaac ggcgagctga cgccaaaccg cgcgttgggc gttttgtaat
    59881 ggtgctgcat atggtaacgc ttgatgtatt tggcgtaccc gccgcgcatg ggaaagtggt
    59941 gcgtggcgta atgggtgagg tcataaagga ggtaaccgaa aatgaaagca gaaaacaccg
    60001 gagccaccca ctgcgccgct ttgaggatga ccgcgaaaaa gaggtaaaaa agagcgtaga
    60061 aaatggccgc cagggggatg gagatcacag gcggcattac caggcgggtt tttaactgtg
    60121 gttgggcgtg atgcacgcca tggaagagaa agatgatgcg ttcggctttt tcgcctttga
    60181 ccggcatatg aaagagaaag cggtgcaaca catactcggc gagagtccac aggaagagac
    60241 cgaccagaaa accaagcgga ataaccaccg gaaaaccgcc agcaggggcg tgggcgatcg
    60301 accagatgag gaagaacagg gcaactggca gccagatgac cagcacggta atggggctga
    60361 tatgagagaa gaactcgaga aaatcagatt tgaacaggcg gatgggaaca tcactgtggt
    60421 cggttggcag gtgcgccatt ttttcctcca tataaataaa ccaggtagag gaattatata
    60481 caggattgac caaacaaggg ggtgaaaaac ggtttgattg ggcaggaatg aaaaagagca
    60541 ggatggtgta aataatcctc ctgctcctgg tgaaaccgat aaaacaggtt aatcctttgg
    60601 cggataaccg gtgatagcac gtatctcgtc atacagaggt ttcaggtggc tgtacatacg
    60661 caggtaaacc cgccggtaaa gctcgttata gatggcgtaa ttttcttgtt tcggctcgaa
    60721 ggtctcgcct ttgtgggtca tttctttcac cgcgctggag aaatcagagt gcagtttaag
    60781 ccccacggcg gcgtcaatgg cggctcccag cccgcttgtt tcgaacacat gcgggcggct
    60841 ggcgggcagc ccgaacacat ccgcggtgag ctgcatggct gccttgctct ggctgcctcc
    60901 accgctgacg cggacttcct caatcggcac atgagtgcgt ttactggtcc gttcagcccc
    60961 ttcgcgtaag gcataggcca atccttcaag gatggagcgg taaaagtgcg cgcgggtatg
    61021 cacatctcca aaaccaatga cggcgccttt ggcttcaggc ccgggtactt tgagaccagg
    61081 tgaccagtaa ggctgcaggg tcagccccag cgacccgggc ggcaccgcgt ttaccaggtc
    61141 atcgaacagt tcttcggcgg ctacgccgcg ctgggccgcc agccgttctt cgtttaaacc
    61201 gaattcgcgc ttgaaccatg agaccatcca gaagccccga taaatctgga tttcgagcga
    61261 atatgaaccg ggcacggctg aagggtaagg aggaatgagg gggatgactt ccacataacg
    61321 gtcgagcgtg gtgttgatgg tggcggtggt gccgaaggag aggcaaggaa tggcgggatt
    61381 gagacaaccg gcgccaatga cctcgcaggc tttgtcggcg gcagcggcga tgagcagcag
    61441 cccctcgggt ataccggttt ctgcggcggc ttcagcggtg atcacaccaa tttgctcggc
    61501 gggtttcacc aactcgggca acatctcctt gttaaccggc actgcctgcc acttccaatc
    61561 gttcttgccg ctccaatctt gctttttgta atcgaatggc aggtagccga caaggcagcc
    61621 aacagaatcg gcgaaccttc cggtcagacg catagtcaga tagcctgaaa gcagcaggta
    61681 tttatacgtg tttgaccaaa tttccggctg gtgtttttgc aaccagttgc cttctgcctc
    61741 agcctgcagg taggccacgg tttcgctcat cccggcgatt ttaaacagca acccccaaag
    61801 accaccgacg ggtttgaggt cggaggtgcg gcgctggtcg agccaatgaa tggcaggtcg
    61861 cagtggtttc ccttccttat cgacgttgat gagcgtggag cgctgcgtgg tgagcgccac
    61921 accagcgatg ttttcttttg ccaccccggg ttgctgccac aaactgttac aggcttcaca
    61981 caacgccttc cagaacacct gtgggtcctg ctctgccagg cccggtgctg gtgaatagta
    62041 aggttcaatg ggcacacggc tgatagccaa caggttgccg cgcaggtcaa agaccatcgc
    62101 ccgggtggat tgtgtgccga cgtcgattgc cagtaaatga tccttcaatt cagccctcca
    62161 ggtccttgct ccaacgctgc tccttctgtt ccgtggtcat atccaggccg agcgttccgc
    62221 cgggattcat gatattgcgc gggtcaaaat gagtttttaa ggcgcggatg atgtccatct
    62281 gcgcgctgcc gatttgccct tccagccagg gagcggtttg cttgcctacg ccatgatgat
    62341 ggctgatagc cgctccgctt tgctgaatgg cagatagaat gccgtactgc agttcaagat
    62401 actgctttat tgtagcgatt ttggcgataa agataaagta caggttgccg ccttgcggat
    62461 aagcatgaga aagatgggtc atgcagatgg tattggggtg gctcttgata aaaccacgca
    62521 cgctggcatg cacctctttc atctgcgacc atgtgacggc gcattccagt gtgtcagtga
    62581 gcacaccaaa atcctgcagg tcttcacgca tgtacgggtc gcgaaaacgg ctcttttccc
    62641 agttactggt gacaggggcg aagctgatgt tgaatgcgcc atgacgccgg cagatgcgcc
    62701 tgacctggcg gttgacgttg cgcgaaaagc ccgcttcacc ttcggtcata cccagcagca
    62761 ggcatttctg catgggcagg tagccgctga gcctgaggac agtgtcggcc ggggtgccct
    62821 cgatgtgata gagcttcatg gccacatcgg tttcttctgg gtcagagagc ctgaacaccg
    62881 aaggattgcc acactcgcac tgcataattt cgcggcaggc ggctaaggcg tcgtcccaat
    62941 tcttgaacat gtaactgaag cggtggcggt tgccgggttg aaggcggaat acgcgcaggg
    63001 tcacttcggt gagcacgcca aacgcgcctt cactgcccat cattatttgg tcaaaatcag
    63061 gcccgttggc gctgcgcgga tgcgggcagg tcttgagctc accgcgaggg gtggcgtaac
    63121 gctgggatat gacaatatcc tcgatcttgc cataataggt ggagttttgc ccggccccgc
    63181 gggtcacgac ccaacctccc accgatgaaa attcaaagga ctgggggaag tgcccgcagg
    63241 tgtagcggcg ctgcgcgcca agccgttcag gcgcgtgatt gaggatttct tccagctgcg
    63301 gaccggtcat gccggtctca acggttatgg tctggtcggt ttcattgaaa gaaaccacgc
    63361 ggttcatgtg ggtactcata tccagcgcga taccgccctt gaccgcttca actccacggg
    63421 taacgctgga gccggcgccg tacacataaa cagggatttg gtgctgattg ctgtacgtaa
    63481 taatctcctg cacctctgag tgagaacgcg ggcttattac cagttcaggg atgttctgga
    63541 tgatcttttt acgcagacgc agcgcgtcga tcatgccgga accataagag cggctgatgc
    63601 gggtgtacgt gtcggttgag atgttttcaa cgccacaaat ggcagtaaac gcatctatat
    63661 ggctcttctc caggagaacc ggtctctcca gttcgaccct gtcaagcgca agatcaatcg
    63721 gctggtctaa atcttcgggc gcaagatcga aggaactcat catcagtttg accaacccgc
    63781 tattcgggtg cttgaacgca gccgggtctc cccactttaa cagtgaacga taagtgcctg
    63841 gaggaacggg ttcttcgtac caattgggtt tgtgtttttt ttcttttacc atggttaagg
    63901 cctcctaaaa accagccggt gaagggctgt aggctttttt atacaattca acatagcgcg
    63961 tgaattcagc ctgccagcgc gcgtcgtccc agccaagctc tggctgcacg atttcgcgca
    64021 cgcgcgccat ttcctcctgg gcgccgtgcg gcagcagcag acccagccgc acacggcgca
    64081 ataataaatc gtccaggtgc tcaaccgcgc cgcagcgggc agcccaacgc acttcagacc
    64141 aaaggttggg caggctgcca acaggctgat tttcaccttc ttcggcgttt tcaaatagtt
    64201 gaatggtttc gttcccataa cgacccaaca gataaagcca ggtttcagca gccattccct
    64261 gtggagatga tcgagtgggc agcggggaga aataggattt tgggcgtgaa aggttggccg
    64321 ggtggggcaa cgcgggcatg gctgcgcgca gcgcgtcttc agccatgatg cggaaagtgg
    64381 tgtacttacc accggtgatg gtgaccaggc cgttctcttc ccaaaccaca tgggcgcgag
    64441 attcttttga cgggtcggct tgaccggtat tgaccacggg gcgcagaccg gagaaggttg
    64501 aaatgatatc agcccgatcg agttgaagcc caggaaaggt ggcgttggct gcttccagga
    64561 tgtagtcgat ctcttcctgt gtggtgtagg gttcaccctg tgagagcggc agggtgtggt
    64621 cgaggtcggt ggtgcctatg aggctcaccc cctcccaggg gatggcgaac atggcgcggc
    64681 ggtcgcgggg gtgcagcaat gtaaccgcgt aaggcaggtt cagacgttgg cgggaaaaca
    64741 ccaggtgcga accgcgcaat ttacgcaggc gcgcgggagc gttaacaccg gcgcgaacct
    64801 catcgctcca cgggccggtg gcgttgatga ccacgcgagc cagtacttct ttctcctgga
    64861 aaccgcccgt ggctgtgtct ttaattactg ctccgcagac cctgccattc gtctctttga
    64921 gcagcgctgc tgctcgcgcg taattaatgg cgcagccgcc agcgctgacg gattcacgaa
    64981 tcaggcgcaa gaccatgcat gcgtcatcca tgccggcgtc gtaatagaga aagccacagt
    65041 gaagttgatc ggcatccagc gccgggcatc ggtcgagcat gtcatgacta ttcagacggc
    65101 tatgatgcca ttttggagcc aacaggtcat agagaataac tcccacggcg aattgcgacg
    65161 ttaacttgcg gtcgtccgga cagggcatta ggaaacccaa gggcgtcacc aggtgccgcg
    65221 cttctttgag cagccattcc ctttcccgca ctgactcacg tgtgacacga aattgcttat
    65281 tgcgcaggta gcgaaaccct ccatggataa gtttggatga cctgctggag gtgccgctgg
    65341 aaaaatcgcc cgactctacc agcagtgtac ggtaaccctc ctgcaccgcg cgccgcagaa
    65401 ttccagcgcc ggtgatgcct ccaccgatga tgaggatgtc ccatttacca tctaactctt
    65461 gccaggcgcg ctggcgccaa cctttttccc acataaattc cctcctctaa ctttacgagc
    65521 ttgtttgccc ttcaatctct tgccaaagtt caaggctttc gcgcatcacg gcgcgtgtga
    65581 cctcttcagc catgaaaata tcattggaaa gcgctgccga gagcagcttc ttataatacg
    65641 atcgggagtg atcgcgggtt aacggggaga gaaaataacg cgaccccatg ctgtggtaca
    65701 ggtctgtaaa accattcagg ataagcgtaa aaatggggtt acccgaaaga atggtcaaac
    65761 ggcagtgaag ctcgaggtca acccgggtaa agaccggagc cgcgtcaggc agggtctgca
    65821 ttgattccag caggcggatg acctcggagg aggcgttgcg aatggccagg cgcgtgtatt
    65881 caggcgcgag cagggcgcgc acatgcagca ggttgggcac gaaatcagca gggagatgct
    65941 gctgatggcg ggcgatagcc gaaaggatga gcaggttgcc ttcctgccag taatcacgta
    66001 cgcgcgtggg acgtccctgg tggatttcta tccaaccatc gcgtgaaaga cgctgcaaag
    66061 cctcgcgcag cgtagggcgg gttacaccca gcgacagcgc cagttcacgc tcaggaggga
    66121 ggggtgaatt gaccgggaaa ttgccatcca gaatggcgga tatcagccgg ctttcggtca
    66181 gttcagcggg tttttgcgga gtggaccatg cgatcatcgc tcaccttggg cagagggaat
    66241 gaatggtaag aggtcatacc aatcaatttc attatagcgg tttatgggtt tttttcaagc
    66301 ccgaatttca ttttatccac agatatacac gcaatttacc aaagatattg gggtagccga
    66361 ccaggagacc cccgcatttt gctattgaat aaatagctgg aataagaatt gcaatacaaa
    66421 aggctctata at
//
LOCUS       DAOTLW010000005        55623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_670_length_55623_cov_11.525680, whole genome shotgun sequence.
ACCESSION   DAOTLW010000005 DAOTLW010000000
VERSION     DAOTLW010000005.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 55623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 55623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..55623
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_670_length_55623_cov_11.525680"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..696
                     /locus_tag="P5516_01545"
     CDS             211..696
                     /locus_tag="P5516_01545"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRT91152.1"
                     /translation="MSYPSTSGRDLVILEQIEQDPDTTQAALASSLEVAVGTVNWHLK
                     RLIEKGYVKVSRLERKKLKYIITPEGIALRARLTVDFIQNSFNLYRLVRERAAHAIQN
                     LKALGYHQVRLEGSGEVAEICRLTCLEHEITLVENESAPVLKLVGLKVILETKEHEHV
                     T"
     gene            680..1633
                     /locus_tag="P5516_01550"
     CDS             680..1633
                     /locus_tag="P5516_01550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-L-fucose synthase"
                     /protein_id="HRT91153.1"
                     /translation="MSMSPDYWQDKRVCVTGGAGFLGSFVTARLHERGAKEVFIPTIE
                     KYDLVQLDSIRQMLDDSNPDIIIHLAAQVGGIGANREHPAEFFYSNLMMGVQLIHEAW
                     LRGVEKFVALGTICAYPKFTPIPFKEENLWDGYPEETNAPYGLAKKMLLVQSQAYRQQ
                     YGFNSIFIMPVNLYGPRDNFKPESSHVIPALIKKCVEAVESGADFIEVWGDGTPTREF
                     LYVEDAAEGILLATEKYDSPEPINLGSGMEISIKHLVELIARLTGLKGRIIWDTSKPN
                     GQPRRRLDVTRAEQAFGFIAGTSFEEGLKKTIEWYRGQKGY"
     gene            1642..2499
                     /locus_tag="P5516_01555"
     CDS             1642..2499
                     /locus_tag="P5516_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91154.1"
                     /translation="MATVNTLEQKRKPPVVVIQPSRGWVGLKLKDLWLYRELVYFLTW
                     RDIKVRYKQSVLGILWAILKPFMAMVVFTIFFGNFAKIPSDGVPYPIFSYTATLPWEL
                     FAAALGVASRSMVSNSNMVSKIYFPRMIIPLASVMSSVVDFFIGFTILIGMMIFYKVT
                     PTIATLWLPLLILLALITALGVGFWSSALMVRYRDVGYIMPFIANLWMYLTPVVYPSS
                     MIPEKWRLLYALNPMTGVVEGFRYALLGTTQSVSGGMILVSSLIAIAVLISGMFYFRR
                     MEKQFADMI"
     gene            2511..3776
                     /locus_tag="P5516_01560"
     CDS             2511..3776
                     /locus_tag="P5516_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91155.1"
                     /translation="MSNLAVRVEDIGKQYNIFQETARYITLREKIVETLKTPVKTLLN
                     PTGSEKFWALRHVDFTLDHGQVLGVIGKNGAGKSTLLKILSRVVEPTEGLAEIHGRVG
                     SLLEVGTGFHPELTGRENIFLNGAILGMKKSEIERKFDEIVAFSEIEKFIDTPVKRYS
                     SGMYMRLAFAVSAHLEPEILVVDEVLAVGDAEFQRKCLGKMSDVASEGRTVIFVSHNM
                     SAIQRLTQECIILDKGAMVLRARTPEAVDYYLTNDFSASGERRYTEEESSSAAYPFKP
                     VAVRVKNPSGQVTDSLFSVDPLTIEVEYQLDADITGLRIGIYMMTSRGEFIFTSFDTD
                     EPEKFQSMSMRKAGHYISRCVIPADTFNEGRFVIGINASSYMVKRYFSDERAMNFSIN
                     TTGAPGMHWVEIRHGPIRPRLDWRIEEIA"
     gene            3777..5357
                     /locus_tag="P5516_01565"
     CDS             3777..5357
                     /locus_tag="P5516_01565"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91156.1"
                     /translation="MASNKELLKKILGELPLTAEVYQLLQQSDRPLPNHFSLRNIRKV
                     LPEAVAAVQKSCAQYPAGKNVFIFASTHYWLEHGVITGLALSGLGHDVTIGYYPYGRW
                     DFNSPQFDVRRQSVYSATVLRPASAVMNFENLLHTHSSFSLLPEALQQAVDQVTDFDY
                     MYAFQTEEVNREDPFYLFRHERNLHAAKSLLTYLRSLKPEVAIIPNGTILEFGIAYKV
                     CGYLKIPTTTYEFSDKRHSMWLAHNQEIMQQNTDELWKTHANYQLTNADLEELKVLLS
                     SRKNASTYGDFSRQWQRVPAEGQAAIQAKLGFDGKPIVLLATNVLGDSLTLGRNTFSR
                     SMSEWIIRTIQYFIEKPEVQLVIRIHPGEALIKHGSILDLIRKTLPTLPPHIHLIEPL
                     AEVNTYDLMDIADLGLVYTTTVGLEMAMRGIPVIVAGKTHYRNRGFTHDPNTWVDYFK
                     LLNRLLTCLRECRLQEDQVNLAWKYAYLFFFKYPLPFPWHMTRLKEDLEEYPLEYVLS
                     EPGLEKFRSTLQLLLSTR"
     gene            5366..6874
                     /locus_tag="P5516_01570"
     CDS             5366..6874
                     /locus_tag="P5516_01570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91157.1"
                     /translation="MMDDQEYENLLFRRQYFIGPKELGISGWNLTHLPDGKILSAHPE
                     LEVTDWNGKHTRYVLIGYIFDPFHPERTNQGILEAIDRCKTDTGSIFEMLEDKSGHYV
                     LFVYDDEMNIALTDACGIKQISYYCDENGKSYYSSQPALIAQKLNLPVSDEAIHYMDS
                     AKYKKKLEPWWPVESSPYKRIRRLVPNHYLNLATNEQVRFWPNKKLKRYSLKEGTILA
                     GELLKGICKACFQRYPMALTFTGGYDSRVLLAACKEFADQIELFSMIYRNLTEQSEDL
                     RIPQAIAAEKQLKYHQIKCDAEIPAGFQDVYERSNQGYKTDWMSLVYGRYIHFPANVL
                     IVKGNIAEVARCSFWQDGIYPVEVTVDTLVDATALGHEPLILQSMGKWLDEAKPTEKF
                     GYKIMDIFAWEVEAGGWQSMSHNVFALSQEEFSPFGNRRLLDIMLGVHKRYRCWPDIT
                     LEQEIIKYLWRDLSQYSYFSSWNLHNYKKKFYDGDLLNFLRKIKLILKRSIK"
     gene            6871..7878
                     /gene="pseB"
                     /locus_tag="P5516_01575"
     CDS             6871..7878
                     /gene="pseB"
                     /locus_tag="P5516_01575"
                     /EC_number="4.2.1.115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 4,6-dehydratase
                     (inverting)"
                     /protein_id="HRT91158.1"
                     /translation="MNWSDSVVLVTGGTGSFGKKFIDIMLKEYHPRRLVVFSRDELKQ
                     FEMQAAGFSHPSLRYFIGDIRDLQRCRRAFEGVDLVVHAAALKQVPACEYNPMEAIKT
                     NILGSSNVIDAAIDAGVSRVVALSTDKAVNPINLYGATKLAAEKLFVQSNAYAGARKT
                     RFACVRYGNVVGSRGSVVPFFLKQRETGRVTVTDERMTRFWISLEQGVRFVIRAVENM
                     HGGEVFVPKIPSMTVTDLARAIAPEAAIDFIGIRPGEKLHEVLISEDEARQTVELEDM
                     FVVQPAESLWFGRDWEGKGRMLEDGFRYSSNNNDQWLNVEQIQSIIAPIEADYLAGRL
                     G"
     gene            7875..8795
                     /locus_tag="P5516_01580"
     CDS             7875..8795
                     /locus_tag="P5516_01580"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT91159.1"
                     /translation="MTRFLVTGASGLLGLNFALAVDGKKHQVTGVANTAPMLWATFKN
                     VQLELTQPGVVDEMIKEHQPEVVIHCAAMANVDACENNPAQARLVNAELPGTIAAACK
                     RHRVRLVHISTDAVFDGKKGDYREEEPPHPLSVYASTKLEGERAVLAANPDALVARVN
                     FYGWSAAGNRSLAENFVRNLEAGKEMMGFTDVIFCPMNILDLSSVLVEAAELDIRGVY
                     HMVGAQAMSKFQFGVVLAEQFGFDTRLIKPVSVNDSGLKAARSPNLSLSTEKIVKALG
                     HELPDFHEGVKKFFDQYRRGYPQYIMSLNG"
     gene            8818..9885
                     /locus_tag="P5516_01585"
     CDS             8818..9885
                     /locus_tag="P5516_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRT91160.1"
                     /translation="MELKIGQHFIGLQHPTYFIADISANHDGSLERAKMLIRLAKEAG
                     ADAAKFQNFRAPKIVSDYGFKAMQGQVSHQATWKKSVFEVYSDASIPFDWTPILKEEC
                     DKLNIDYFSSPYDFEAVDYLDPFVPAHKIGSGDIDWLEMLEHIALKGKPVILSSGAAT
                     IGEVQQAVHTILPLNQQLVLLQCNTNYTASPVNFDHIHLRVLQTYQTLFPEIPVGLSD
                     HTHGHATVLGAVALGARVIEKHFTDDTSRVGPDHPFSMTPVTWREMVDRTRELERALG
                     SADKSVSGNEVDTTIVQRRCLRASRDIRSGEVFTREMIDVLRPATPGAIRPDQVFQVV
                     GTRALKDIPMGKELRWTDLGE"
     gene            9887..11014
                     /locus_tag="P5516_01590"
     CDS             9887..11014
                     /locus_tag="P5516_01590"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT91161.1"
                     /translation="MKVVYFTRDYSPHDERFLTALAQTQHEVYFLRLSPSSEVHLPPG
                     IREVKWVGPLAGEIISDDRRAEDLNQLLSRLQPDVLHAGPLHGPAYIAGLSGFTPLVS
                     MSWGSDILHDGEVDALARAKINLSLHRSTVFLGDCQAVVEKAINAFAFPAERVFRFPW
                     GVDLAHFSPSTTSPLRASLGWQQQFVFLSNRSFEEIYGVDVVMRAFIQAQSRAPNIRL
                     LLYGKGSLQQEIMRMAENAGVMHKIHFAAFAGHEDLPASYHAADVFLSASHCDGSSVS
                     LMEALACGKPALVSNIPGNLEWVHHGENGWLFQDGDTTGLADAMVAVSRKPNLEKLGQ
                     KSRALAEEKADWSCNFAVLLQAYQKAVEWRTPALEITERVK"
     gene            11011..11766
                     /locus_tag="P5516_01595"
     CDS             11011..11766
                     /locus_tag="P5516_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HRT91162.1"
                     /translation="MKVVAIIQARMSSTRLPGKVLKLASGRTMLDRMLERVKKARTIS
                     EVVTATTTDPSDDSIARACKKLGVSVFRGSLQDVLDRFYQASRMHQADIIVRLTGDCP
                     LIDPALIDDVVNALTRKKVDFACNRLPPPFSRTYPIGLDVEACTFNALEQAWYGAKEK
                     FEREHVLPYLYAVPGRFSVLQLNHTTDLGHLRWTLDTPEDLVLLRRIYRYFQGRNDFS
                     WLQVLQLQQQKPSLFAINAQVRHKTYLENESPR"
     gene            11778..12626
                     /locus_tag="P5516_01600"
     CDS             11778..12626
                     /locus_tag="P5516_01600"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT91163.1"
                     /translation="MSEKIVCPRCQSSFDKHDPAVWSCSSCAYTVPLFQGKPVFSGHP
                     QEQQPQDKIERGADKGTAWRRANWRFIHAIAASLPAGADVLDVGAGRGDFRQIFAHHS
                     YIGLDIYPYPEIDLAVDLIEINPFKERSFDLVVLANVIEHVYEYRKLISRCAFLLKPG
                     GQLLITVPFLLKLHQEPVDYHRYTHYALFQLAEENQLSVARFEVYTNPFALLDEGIAD
                     VWENVLPLRQRIDHLHTKVKVALIQKLANSMKANQADGVVKPVQENSNPRVLGYMCLF
                     QKDALQ"
     gene            12623..13555
                     /locus_tag="P5516_01605"
     CDS             12623..13555
                     /locus_tag="P5516_01605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91164.1"
                     /translation="MTSVAFFTAPKPFTNAHIRIIQRNAIQSWKSLGRQVEVWLVGDE
                     PGVEQNARELQVGYIPGVARNSFGTPRIDSIFHLVREQSQAEYLCYVNADILVFPDML
                     RALEEVRARVDRFLIIGRRWDAAVTETLDIKEGWHQQFQAAALVTAKLHRAAGSDYFL
                     FPRNEFIDIPGFAVGRAGWDNWMIYKARYEHMAVVDATGAIKVIHQNHDFSHFADGKI
                     HRLQPESAENMRLAGGRYTVFTIHDANYQLDNDGLQRVRLNRWKLLREFVIFPAVTLH
                     CSWLAKVVYAIFNPKRLLKDRRQAKEALRRSKEG"
     gene            13556..14512
                     /locus_tag="P5516_01610"
     CDS             13556..14512
                     /locus_tag="P5516_01610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT91165.1"
                     /translation="MRKGQNPVKQLQTVSKPQRVTVAVLNHIPFLDGFYAQMLDVLQL
                     SLSTLRQNAGLDFDLLVYDNGSCKQARDFLLAEYEKGNIQSLILSDRNMGKGGAWNVM
                     LQAAPGEIIAYADNDVFYHPGWLRESVKVLETYPNVGMVTSRPFHTKPELYSADLDWA
                     RKTPEASVERGFFIDRDWMREFWLSVGRSEEEIALDQAVEDVRLTYKGLVTYCGASHW
                     QFLAWKKVLQEFLPIDLSRPLGQVLKLDEMINQQGYLRLMTAQPFTMNLSNSVELPSN
                     EPTSAAAQPRTRKRLADFPVVKSPLMRLYNAIFKLYYDDKKR"
     gene            14523..15680
                     /locus_tag="P5516_01615"
     CDS             14523..15680
                     /locus_tag="P5516_01615"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT91166.1"
                     /translation="MKILYATGRYSPLKHDDGSGTDYRLYHALLSQGVEMKFIGPFPD
                     HPSLIERLYRKVHTVFSHKRPAKYTAAMLKTAAQTLSITVNEYQPDVIFSKNLAPLVR
                     FHAQLPVVYMLDSTVAAFNRGWPTFSRLENWRMVEWEKKVLKQSSSVITRSEWTRQSL
                     IEDYHYPAERIHIVHNGSSLPDHVIPQEIENEPPDFSTIRLLFVGRVMHLKGIDIAIE
                     IVNQLNECGRQADLRIVGLDGQDQDHIHYMGSFKKSIEDQLIAYANQYRWPHFLLHPA
                     RYDAAPIVTAEAAAFGVPTLTNNVGGIASMVQDGLSGVVLPALSPAAEYVKKIVYYAD
                     HPQEYMALRRTTRERYEKELNWNVAGKKIVTILQSVVEENQLKPGLKKEQP"
     gene            15677..16660
                     /locus_tag="P5516_01620"
     CDS             15677..16660
                     /locus_tag="P5516_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRT91167.1"
                     /translation="MKILIAGYGSIGRRHLNNLRALGQTDLLLLRSHRSTLSDVDIQD
                     VPVETTIEAALAHQPRAVVIANPTALHLDVAIPAAQAGCSILMEKPVSHSMSRVEELR
                     RALTSGGGVLLSAFQYRFHPGLRQVKRWLQEDQIGVISSVKSHWGEYLPGWHPWEDYR
                     SSYSARVDLGGGVVNTLCHPFDYLRWMFGEAEQLVASTSSRGLNLPVEDTADVLLRYQ
                     GGVCANIHLDYLQRPGQHDLLITGSAGTIRWSNTDGSAKRYDPIHDQWEEVRPPEGFE
                     RNTLFLEEMAHFLRVVERQEAPLCTLEDGIAVLQLTEAVHRSAREGCLVKF"
     gene            16679..17500
                     /locus_tag="P5516_01625"
     CDS             16679..17500
                     /locus_tag="P5516_01625"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT91168.1"
                     /translation="MTSIFEKFSLKGRVALVTGGAGLLGKEFCLTLAQAGAQVYVADL
                     NLSAAESAAQTITAQGHSACSIRLDVTNPDSVQSVVDEIVSRSGSIDVLVNSAAMDPK
                     FDPGHAQTNSNAFEAYPLGSWQDALNVNLTGPFLVSQAVCRQMVRQGKGSIIHICSTY
                     GLGGPDQRIYEKPGQSKVFKPVYYTVTKAGILGLTKYMATYYAGSEIRVNALTPGGVF
                     AGHDDVFVKNYSARTVIGRMAHKDEMNGALLFLASDASSYMTGGNVVVDGGWTAW"
     gene            17494..18750
                     /locus_tag="P5516_01630"
     CDS             17494..18750
                     /locus_tag="P5516_01630"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HRT91169.1"
                     /translation="MVKKPEVLALIPARGGSKGIPRKNIKLFAGFPLISYSIAAGRQA
                     QSVTRVIVSTDDEEIAAEARRWEAETPFLRPPELAQDQTTDLPVFQHALRWLRENEGY
                     TPDLVIQLRPTSPIRPRLCVDEAVALLRNHPGADSLRGVVPSGQNPYKMWRLQGKDEP
                     MTPLLKLDGIAEPFNAPRQSLPPTYWQTGHIDVIRPRVILEGNSMSGRNILPYLIDPR
                     YTVDIDTPFDWLRYEWLVRNADLDMVDPASKRRKLPAKISLVAMDFDGVLTDNLVYVD
                     QDGSERVASSRGDSMGIRLVREALPVNFVVISTETNPVVSARCRKLNLEVYQGVNDKP
                     AVLKQLLQERQVPAGEVVFIGNDVNDVGCFDLAAYAVAPADAEPEVRRRADLVLTRKG
                     GKGAVREFCDLLLQNAGKILPTQEKK"
     gene            18747..19622
                     /locus_tag="P5516_01635"
     CDS             18747..19622
                     /locus_tag="P5516_01635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HRT91170.1"
                     /translation="MTREVKIGNKWVGDGHPVYIVAEVGINHNGDIEIAKELIRAAKH
                     AGVDAVKFQKRTPELCVPPEQRGQMRETPWGYISYLDYRYKVEFNEADYDEINRYARE
                     IGIEWFASVWDIPSVDFLEKYEPICYKIPSASLTDVELLLHLRKTSRPLILSTGMSTQ
                     QQIKDAVKRIGEENLVITHATSAYPCDPYELNLRMIQTLRGIFNCPIGYSGHEVGLVT
                     SAVAVGLGACMVERHITLDRALWGSDQAASVEPGGFEKLVKYIRVTEAAIGDGVKKVY
                     ESEMPSLRKLRRIQD"
     gene            19619..21229
                     /locus_tag="P5516_01640"
     CDS             19619..21229
                     /locus_tag="P5516_01640"
                     /inference="COORDINATES: protein motif:HMM:NF017011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91171.1"
                     /translation="MKKAFLRVRNGIRQRWVHKKANQRWRALATAITLQSPLPSDPRP
                     VIFFNASTRLQAMSQNAAYALLTAQALRLQGVPVIQFVCGGALQRCVLGSQQDNFNLP
                     PPCQACTRQSQAVFQELNTRWMETREHPMLGKELETLSFNEYETFTYQGTPLGAWAVN
                     SLRWMMRRHHLQEDETTRAFFRSFILSSWNVFTQFSELLDETQPRAVVLFNGMFFPEA
                     AARHACLERGVRVITHEVGLRPFTAFFTTGEATAYPMIIDPAFQLDDQMEKKLDRYLS
                     TRFRGEFTMAGIRFWPEMKALEADLLEKINHYSKLVAVFTNVIFDTSQVHANTLFEHM
                     FTWLEHVKTTALSHPEVLFVIRAHPDECRQGKESRESVATWFSSSEISRLDNVVFIGA
                     DEYLNSYDLIRRAHLVMVYNSTIGLEAALLGKPVLAGGKARFTQLETAFYPQSAQEYT
                     VMLERLLATPSIDTPPEFARNARRFLYYQLYATSLDFSDYLEEDGVWKGYVKLREFSP
                     DLLKPDNSLTMKVLLNGILENGDFTLPL"
     gene            21226..22170
                     /locus_tag="P5516_01645"
     CDS             21226..22170
                     /locus_tag="P5516_01645"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91172.1"
                     /translation="MTTPVCSIIIRAYNEEKHIGRLLRGIQEQDLHDVQVILVDSGST
                     DGTRDVARAYPVEIVEIDPREFTFGRSLNLGIAAASAPLIVMASAHVYPVYPDWLTKM
                     LQPFQHTEVGLVYGKQRGTGTTAFSEHQIFSHWFPDVSVKKQSHPFCNNANAAIRKEL
                     WQQHPYDETLPGLEDLEWGKWAQEQGYTISYVAEAEIIHVHNESLDGIYNRYKREAMA
                     FKRIYPHETFTLRDLNRLFLQNVISDWREARRQKVFAHHFFHIIGFRWRQFYGTYQGY
                     RQSGPLTWQLKRAFYYPRDFRQASGQTSRRDIEPIKYN"
     gene            22205..22492
                     /locus_tag="P5516_01650"
     CDS             22205..22492
                     /locus_tag="P5516_01650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91173.1"
                     /translation="MAHVTIEFTGLSKSITGVNEVNLPINPTDTYRDVVRQLGTLYPA
                     LVGILIAEDGETFLSSNMFIIDGDYANPAMVMENSPRDGEHIHLMSVITGG"
     gene            22518..23198
                     /locus_tag="P5516_01655"
     CDS             22518..23198
                     /locus_tag="P5516_01655"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HRT91174.1"
                     /translation="MPEKLQHPKISALVPMRHHSQRVPGKNYRPLNGIPLYQHILTTL
                     TQVPEVDEIVVDTDSDIILHGIQSNFPAVKVINRPPELRADDISMNTILLHDISQSPA
                     DLYLQTHSTNPLLKASTISRAINKLLQVYPEHDSLFSVTRLQTRLYDHLGKAINHDPK
                     VLIQTQDLPPVYEENSCIYLFTAATLRKNGTRIGETPLLFETDPEESWDIDEEVDFKL
                     VEMILKAR"
     gene            23202..>23827
                     /locus_tag="P5516_01660"
     CDS             23202..>23827
                     /locus_tag="P5516_01660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91175.1"
                     /translation="MKRFSSFSVLLQLGIFLLGVSGSIYVAFTPANSMMNWYSNDDAF
                     YYYKVAQNVLSGHGFSFDGINLTNGFHPLWMVICLGVFWLSRFHLLLPLRVLIVISGV
                     LNGLTGVVLLRLLQKFLPLVAAILGACVWILLPSIYNNYTAHGLESALSAFFVAVLLL
                     KSADLLTGPAEKRAGKLIILGIIAALTILSRLDTLFVVAMVGFFVVFK"
     assembly_gap    23828..23927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <23928..25015
                     /locus_tag="P5516_01665"
     CDS             <23928..25015
                     /locus_tag="P5516_01665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91176.1"
                     /translation="FYRPSSQVLSVSAWQQVKNWIRQGLPHHLRDGVLFSFPIVLLVC
                     GYMLTNKLEFGTFIPVSGQVKTWWGTLENTVYRQNTTLVGLMGLAPGRGKSPWSLLTS
                     IVADISIFIRNLFVKDTEDLPVILFLVFLFMLFILLIFLLSRKDGLLARRSFALLIPA
                     LAGGCLLHIAYYAARNYGHTRSWYWVPETMLLVLLGAVVSSRMFEKTRQWTRTPVAEN
                     LLLLVMVGFVFFLHARYIARLCPPSVSPEHQADYLAETRELESYTRENSLIGMTGGGA
                     TAYFIQNRTIINLDGLINSAEYFSTLKTGATNRFLNNLHLDYVFGNSYMLLKTDPYQQ
                     IFTGRIREIGNIHSGSHFTLYDYIGDQ"
     gene            25087..26058
                     /locus_tag="P5516_01670"
     CDS             25087..26058
                     /locus_tag="P5516_01670"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HRT91177.1"
                     /translation="MPVLLLSAPYMLPFVNRFKPVFDHYQLELLLAQVNERLGEEALL
                     SLAGQFDGIICGDDRFTPRVLEACSPRLKVISKWGTGTDSIDRAACERLGIQLKNTPN
                     AFTIPVADSVLGYILAFARRQPWMDRAMKSGKWQKIPGRALSETTLGVVGVGNIGKAV
                     LKRASAFGMRLLGNDIIPIPADFLQEYHVESVSLPELVSQADFISLNCDLNPTSRHLI
                     HKDIIRLMKPDAVLINTARGPVVNEKDLIEALQQKRIAGAGLDVFEEEPLPADSALLH
                     MENVLLAPHNSNSSPRAWENVHWNSIRNLLIGLAIDAEDLDEWKSKV"
     gene            26073..26837
                     /locus_tag="P5516_01675"
     CDS             26073..26837
                     /locus_tag="P5516_01675"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT91178.1"
                     /translation="MKENKRVVLITGSAGGIGKACVELFAANGWQVVGIDVNAFGPGF
                     PADGLYIQADISQPEEFPVLFEKVSAFSTRLHALVNNAAVQVSKPLIETTVEEWDKVI
                     ATNLRPVFLSAREFHPLLINAGSSAIVNMSSVHAIQTSASISAYAASKGGMLALTRAM
                     AIEFAKDNIRVNAVLPGAVDTPMLRSGLDRGHVTGPSLNERLDNLARKTVNGRIGQPA
                     EIARAVYFLADGTQSSFMTGQALVVDGGATARLSTE"
     gene            26844..27647
                     /locus_tag="P5516_01680"
     CDS             26844..27647
                     /locus_tag="P5516_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF115 domain-containing protein"
                     /protein_id="HRT91179.1"
                     /translation="MRAKIKQLIPAPLVDLYRSARDNLSLAFAYPNARFHPWRVDSIQ
                     KLEALRDSHRGERCVIIGNGPSLKQTNLQKLRGTFSIGMNRFYMAFNDLGFTTSILLT
                     VNNLVIEQCADELRGLPIPIFVSWRGRRYIHPAPNLHYLYTSYIKPSFNGDATGRLWE
                     GATVTFVAMQLAYFMGFKQVILIGVDHNFTSTGKPNTTVVSTGADPNHFHPGYFGKGF
                     RWQLPDLETSEIAYRMAKSAYEKDGREILDATIVGKLTVFPKVNFDTIW"
     gene            27647..>28563
                     /locus_tag="P5516_01685"
     CDS             27647..>28563
                     /locus_tag="P5516_01685"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91180.1"
                     /translation="MKSAPSVTIVTPSYNQAPFLEQTILSVLEQDYPNLEYFVVDGAS
                     TDTSLEIIQKYAHRITWWVSEKDNGQADAINKGLRRATGDIIAWINSDDYYLPGAIQM
                     AVQALQENPSSSFVYGDVQVVNEKDELINILRYGDWGLKELMSFHIIGQPAVFMRRAA
                     LQKTGYLDLSYQFLLDHQLWLRLALHGGMTHVPQLWAGAHYHAGSKNMAQAALFGNEA
                     QRIVQWMEATPEFASLYTQNSRRICAGAERLNAFYLFDAQDYRASLDAYLRSLALHPA
                     AAAQDWYRILFAALSPLGLDQLKKAHLARR"
     assembly_gap    28564..28663
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <28664..29965
                     /locus_tag="P5516_01690"
     CDS             <28664..29965
                     /locus_tag="P5516_01690"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRT91181.1"
                     /translation="ITTGRSEPLIEPPVMEYLSAYIYAVIGKESMLVPRLLSIAFWIV
                     GGIPLFLLMRKITTLNGAFISLALYLFNPFAIVASRSFQPDPLMIMLILWALYFQFKW
                     MEDDKLASAIFAGIFTGLAVLIKTPAVFFVGIPMIGVVLLKGLKKSLRDWRVYLVAAI
                     SLLPAVIYTALSATVGGNAGAIFGARWVPALFSDPKWYLNWLMLARLVVGFFPIVISL
                     LGFFLIQDKKYQALYFCMWLGYLLYGFMFAYHIYTHNYYHLPLIPVVAMGFGVIGGEL
                     FSRLESKNLSRFFRLLVLLLFLFAIGLNVMKARGEMLGKSYRHEAKYWKELGEKIGQN
                     ANTIALTHDYGYRINYWGFVQPTLWPTQGDMTVKKLIGSTDPEFEALFKMRTEGKDYF
                     LVTLLGDFESQADLHQYLFENYPYEQGDGYYLFDLKHPLGN"
     assembly_gap    30113..30212
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <30213..30590
                     /locus_tag="P5516_01695"
     CDS             <30213..30590
                     /locus_tag="P5516_01695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91182.1"
                     /translation="AAQTDLKRLRRGYVHIPQPATFFRAELWRQVAPLDDRFYFAMDY
                     DLWVRLARLTEFKYLPGKRWASFRLHSDAKTIRADERCWPEMLRVHYRDGGSFFSPIV
                     LKYLLRKLAAPLIRWKRRRMFKR"
     gene            complement(30636..31817)
                     /locus_tag="P5516_01700"
     CDS             complement(30636..31817)
                     /locus_tag="P5516_01700"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008890 - glycine C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HRT91183.1"
                     /translation="MSKRLDWVQEEIDTLKAAGLYNHIRTLGSPQGAWLVVDGKEVLN
                     FCSNNYLGLANHPIMVKAAQEALKKFGVGPGAVRSIAGTMQLHTELEQRLARFKGVEA
                     VVVLQSGFAANTAVIPVLVGKDDLIISDELNHASIIDGCRLSGAPIAKYAHTDVADLE
                     RVINENEGKYRRALVITDGVFSMDGDLAPLDKIYEVTSRHDIMLMVDDAHGEGVIGRG
                     GRGIVDHFGLHGKVDVEVGTLSKAFGVVGGIVAGNARIIEWVRQRGRPFLFSSATPPA
                     DVAACIAAVDLLESSTELVDRLWENARYFKEQLKGIGLDTGQSVTPITPVMLGEAPLA
                     QQFSRELFTEKVFAMALGFPTVPQGKARIRVMISAAHNREDLDFGLAAFKKVAKKLQV
                     I"
     gene            complement(31861..33495)
                     /locus_tag="P5516_01705"
     CDS             complement(31861..33495)
                     /locus_tag="P5516_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91184.1"
                     /translation="MPGLRSTLANYDLDFLTRIARRWDVQISQREVESARQDLLENML
                     DTSLFEKVLEGLDDGAAKAWNHLVQKGGKIHWAEFSRIYGQIRDYGRASREREEPDLH
                     PVSAAETLWYAGLAGRAFLRTEAEPKEFFYIPDELLEVAKNTGKPAPKLHIRPSVNQK
                     PKWVARAEALLPDRVTDILAALRMRREIPAEIWNAWDIPRDFLYPWLREVGLVDDTLQ
                     PVPEALKEFFNATRGQVLTHLYQVWLKSNEINELRLLPGLEFEGTWKNDPIPPRRLLT
                     EILAGLESGTWWSLSSLLATIKETNPDFQRQGGDYESWYIRETKSSTYLSGFENWEKV
                     EGALLTYLLGAPLHWLGIVNLARAGENGKITAFQLNEKMKPLLDGAELSSSDEENRVM
                     KIRDRLLFSIPYFAPRLPRYQVARFCELVHVFPKESLYALKTDTLKKAEEQGLRLGQL
                     IQLLEREKAAAIPENLRVLEERWSQHGQEAVIERVLLLRFTREDARAAFLKQATGRFA
                     LETLDPLCVVINEKQVDGILRLLNELGILAEIAGDV"
     gene            33633..34448
                     /locus_tag="P5516_01710"
     CDS             33633..34448
                     /locus_tag="P5516_01710"
                     /inference="COORDINATES: protein motif:HMM:NF025690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HRT91185.1"
                     /translation="MALKGNLKDFSFIQLLNLVNLANKSGALYVERPGESARVLFHNG
                     KLEYFESSNEKPELLRSLKDQKLITAYQHTLLKERLEGFSDREAGIYLVNAGYATLDQ
                     VTTALEEDYAKKFRALFDWNEGLFRFEAGELLPENKIPVHLNLENLIVEGARELQEIE
                     ELKAEIPSLDMAIKFTDRPGTDIRNICFSPTEWQVISYVNPRNTIQQIATATKLDEIE
                     IRRVVFTLLQAGLVEIIRPGGVPLAIPGKNLPVRNPEENRSLVKRLIDRIRSI"
     gene            34470..35045
                     /locus_tag="P5516_01715"
     CDS             34470..35045
                     /locus_tag="P5516_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP/GTP-binding protein"
                     /protein_id="HRT91186.1"
                     /translation="MQTQTVKIVVTGPFNAGKTEFIRSVSEIDVVSTEKRISATAEKI
                     KESTTVAMDFGRITVDNDLVLFLFGTPGQKRFDFMWEILSEGMLGFIVMVDSTRPETF
                     REARSILETFKAYAPTPYLVTANKQDLYDAWDTEDMRLALRLDPSVKILPCEATKKSS
                     VKRVLLELLYGILKEMDDNEARQNNQEDEIS"
     gene            35047..35547
                     /locus_tag="P5516_01720"
     CDS             35047..35547
                     /locus_tag="P5516_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91187.1"
                     /translation="MSQTMLEYKNVGLVEVLGAEAIADFAKAMEAQPCDPIVHGILVR
                     AGRAGFHYWLTQEGDTLEKADASFRLAPIKRKINLGLQHFCAILSTDTKYALEFKDSA
                     NKWELALTAVEPAGLFSLESSYLSGFLQEFASWAGMGRLYQVHKKVGDSPHAQHSIII
                     EKEPVE"
     gene            35604..36161
                     /locus_tag="P5516_01725"
     CDS             35604..36161
                     /locus_tag="P5516_01725"
                     /inference="COORDINATES: protein motif:HMM:NF027788.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRT91188.1"
                     /translation="MTEDVFICVDVETAGPVPGKYAMLSIGACLVFDTTATFYIELKP
                     DKPEVDPEALAISGLDMDALQQNGVEPAEAMRAFADWVKVVSPAPAKPLFVAFNAPFD
                     WMFVNDYFHRYLGRNPFGHSALDIKAYFMGQMGVNWADTTMRRISSFFLEEHRLSHNA
                     LRDAQDQAEIFRRILEANPRFNLAE"
     gene            36236..37567
                     /locus_tag="P5516_01730"
     CDS             36236..37567
                     /locus_tag="P5516_01730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91189.1"
                     /translation="MAKTNDSHKADLNRIIESAKRMGVEIKEDEALQWLTNMAAYKES
                     GQISLDTRSGVFGHNITMLDFSDKELEYFRRIGKIVEFEDIPGKVETALALSGSSAQS
                     KIQTYPGDCDYFERINIIAPTRDEACNILAEIMRKKALDYLQGPNYQLIEVKFGEYPQ
                     DIVLGTREVKAGSPISWDPAQIREGRITGFKKDGTPVTVAWDDVKNNPGWCKLDWVVA
                     DPIRKQLANASNMLDVTWEAPGGDITPLDGYLDPYFQEVYLEASSVPIFSKLSQHVSA
                     NALEEYVNALEREVYKYLTKDVNYGKVAKRMYNIFRLTGKYEEAAFLRELFDEPSTVL
                     YQIWSLIRTIDDAYIPGSSITNEQMVESTDRLILEVIRALEGDEETEIAKHLLNLRTA
                     LANPDARDMLTAEAEAARARVINIVNNFFYEKLVAYPTIKEYMDEVQTRSH"
     gene            37757..38323
                     /locus_tag="P5516_01735"
     CDS             37757..38323
                     /locus_tag="P5516_01735"
                     /inference="COORDINATES: protein motif:HMM:NF024952.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2'-5' RNA ligase family protein"
                     /protein_id="HRT91190.1"
                     /translation="MNNSHQSISEGVAVELYLDAAAEEQVLKFRELIYREGVQPVQGL
                     MDDKPHISLAVFPITDPAAVIDLTREFALTVPRFSFRLGAVGTFPTQDNVLFLSPVPS
                     VPLLQVHAAFLTRVKDAGINCSPYYFPGVWVPHLTLEFEISNDELCRSMKVFKESFTP
                     IDGEFTQLGVVQFRPIQYLDQFDLQKGN"
     gene            38323..38772
                     /locus_tag="P5516_01740"
     CDS             38323..38772
                     /locus_tag="P5516_01740"
                     /inference="COORDINATES: protein motif:HMM:NF018508.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HRT91191.1"
                     /translation="MSEGVPPPSEEQIRDAVNRRKTKAEEYLQDPDKTRRLLEEAVHK
                     ANDHEGKPGQKLDFWLQLKAFFRLLRAYTNREYTVVPWGSIVMVTIAILYFVSPIDLI
                     PDWLPLAGFVDDAAVILFVIRQLKQDIDGFLAWENAQKNPGGQIIDL"
     gene            38844..39221
                     /locus_tag="P5516_01745"
     CDS             38844..39221
                     /locus_tag="P5516_01745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91192.1"
                     /translation="MSKKWVTVLLVVFVLMFALSACGSLKDVSKLGDKFMSALKTQDN
                     ETSYAMLSPDIQYEVGYEAGWAEWTSIRAFNDWKFTSKSVENNLATLEGTAHLDGEEY
                     LVTLAFTRNGDAWEITTIIFELP"
     gene            39433..40368
                     /locus_tag="P5516_01750"
     CDS             39433..40368
                     /locus_tag="P5516_01750"
                     /inference="COORDINATES: protein motif:HMM:NF013921.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - transposition, DNA-mediated
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HRT91193.1"
                     /translation="MKINLLAFSADRQLLTSLEVITFNGITLTTTPAGTSSELRALTQ
                     QQRFDAALIDAADGHLPVVQTILNLTAQQPGIKILLLVADEQNETLPPEDIAYDRLLS
                     RPLSPEQLISSLKELFLQPEETCAETGAAIRETPAAQAEQEPSLIEIIDAAENENRLT
                     GEQGGALTSHTVKSAGNADPNLIVKELRLSYCCVLLPWSPHQYLTGELSDHAADFLPR
                     LHHSLGWRMTGISIRPQYLQWVISLPVETCPVTAIQKIRQRTSANFFKTFPDLPSSGE
                     NRDFWAPGYLMLSGVQTVPSAIIHEFVKRARPADG"
     gene            40389..43175
                     /gene="polA"
                     /locus_tag="P5516_01755"
     CDS             40389..43175
                     /gene="polA"
                     /locus_tag="P5516_01755"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HRT91194.1"
                     /translation="MPPILYLIDGHALAYRAYYAITAGMSDRMQTKTGEPTAGIFGFA
                     NVLMRLLEQEKPEYLAVAFDTGKTFRNELFPDYKATRAKMPDDLRPQIERIRQMIDAF
                     HLPRLEKEGVEADDVLGSIATQAVEQGLGVKIITGDRDLLQLVNDRIIVNLPGSRLAD
                     AKNYSAADVKEYLGVEPEQVVDYKALVGDTSDNIPGVPGIGPKTAVTLLQTYHTLDAL
                     YQHLDEIPGKTGEKLRVGKESAYLSQELARIKKDVGVRLVLEDARTDHLDIASVENLF
                     RELEFRTLIPRLRLIAAPLKKQETPQLSLFGEEMKEIIVPESSYMIEVKLIDTAQKLA
                     SLKQALDASSLIAFDTETTALDPLQAELVGLSLAVKEGEGYYIPLGHKGEQPQLKVSE
                     VIEALTPALTNPSIEKAGHNIKYDALVLARYGLRVSPLSFDTMIAEWLTNPASRDLGL
                     KDLAGTLLGIRMTRIEELIGRGKNQLTMDEVPIAAAAPYAAADAEVTLRLIPLLKEKM
                     QKTNSTGIFNTIEMPLVPVLLKMEQEGISLDIDFFKRFSAEIGERLRLIEDEVHQAVG
                     HAFNLNSSQQLSKVLFEDLKLEPPDRNKRTSSGHFSTSAAVLDELKGQHVVVDLLLEY
                     RELSKLKSTYLDALPQQINPRDNRVHTSFNQTGSVTGRLASSDPNLQNIPTRTETGRQ
                     VRLGFIAAPGHRLLSVDYSQIELRIVAHMSGDEAMLKAFRDGQDIHATTAAAIYDLPL
                     EQVTKEQRRHAKAINFGLIYGMSAFGLSRSTDLTLGEAENFVRDYFEHFPGVKKYLDQ
                     IRVLAARQGYVETMLGRRRYFPNLANPLNQMMRNREEREAINAPIQGTAADIMKIAMI
                     RVQRALEESASRARMILQVHDELVLEVPENEIALTSQLVRGIMEAAYKLDIPLLTEAR
                     SGVNWGSMQVIASE"
     gene            43194..45515
                     /locus_tag="P5516_01760"
     CDS             43194..45515
                     /locus_tag="P5516_01760"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT91195.1"
                     /translation="MNVTDQALQAKLNNGHAALWEHDWDNAVRVYTEVLLSAPENPIA
                     KASLGLAKFHQKKYIEALQLFQQLAAQYPEDPMPMERIARIYEREGLLAEASTSFFRA
                     AELHLKAKDVEKSIADFQAVIRIEPNNQIARARLALIYERLGRKNEAAAEFLDLAAVI
                     QQHGNIDKARLMLEYALKLKPDAVEVHNAMASLKKNEPLRLRDRSGEPTGAARMAQVR
                     EIETAVQEPEELTGNDPMTEARVMALKEMAGFLFEDQETNRPAVKPRDLQNFVPASSA
                     SEKAEAASHKRVLYHISECIDLQTKGENLEAAAELERAININLDLSAAHYLLGYLLRE
                     SDPGKSYPHLQKSVMDPAYALASNLIMGEIHFEAAQLKEASSNFLHALMLADAETVPP
                     EEAGDLLQLYDPIFESLAFITHERDLRNLCAVIREQLSRPDWRDYLKAAREQLPPAPE
                     GSPPLPLAEMLMDSTSSQVVESLAEVRQLAMQGKNRSAMEEAFRALSYAPSYLPLHIQ
                     IGEILVNEGRIQEAVEKFIQVARLYTVRGDAAQAIRLLTRVTRLVPMDISVRRNLIDL
                     LRSNGRNAELIEQYMDLANVQYLLADLEQSRNAYHSALMLARQSRSTREQSIKILNRL
                     ADIEMQSLNYKEAVKAFEQLRSLSPLDRSTRVALVDLYQRLEMESAALNEVDAYIKLL
                     EDENRLGDAENFLDAILSEKPDSDGLQRCMVSYYTSHKKTSTAIEKLDKLAEKLLVEN
                     NVKGSLAVVEKIISLNPPNQTEYERLRAELLKR"
     gene            complement(45577..46530)
                     /locus_tag="P5516_01765"
     CDS             complement(45577..46530)
                     /locus_tag="P5516_01765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91196.1"
                     /translation="MRKLIRLLVIVIVSALLGASSSGAVRAQESDSVYVPETGHWIWG
                     EFLRTYNSVSDPLLYFGYPITDDFTDPISKSRVQYFEKARFDLVETPSGPVVRVAPLG
                     ELLHEAGNPLANIPREGPACKAYQTGFTVCYAFLQYYEAHAGETHFGMPLSNVEVEDG
                     RYVQYFTRARMEWWPDRAAGERVVLTDLGKLYFDRVVADPELLKSNPPPNLSGSLMTP
                     RVRVFTQKSLIGVEESQSVFVVVQDQYARPIEGAQVGVTLSFMPDTREFYRLPETNEH
                     GISQFTFTTPQIPVKSVVKVLADITFRGQHGSGRSWFRIWW"
     gene            complement(46557..47951)
                     /locus_tag="P5516_01770"
     CDS             complement(46557..47951)
                     /locus_tag="P5516_01770"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT91197.1"
                     /translation="MLLERDRLNGFNLLEKKGSVNGRLPWIGEVRVRIMRVISRFSTI
                     LKKYPRQFWLVSLIMLLAWLFHSLIWPYLMLYISQRLGEPLSSVAWLMTLNAAVGMVT
                     TFMGGAIADRFGRKGVMVFSLIFSALGWFLFRAAGTLPVFALLLVVTGASTPLYRLAS
                     DAMVADLIPEANRLDAYSVLRMGNNFGVVLGPAIGGYLASISYGITFGVIGVGFGLIA
                     VFVTLLIIETKPASGKPVVVTAPTSGGYLRIFKDRVFMVMMGAYTLNRISTSILWLLL
                     AVYAKQNFGVSEKMFGFIPMTNALMVVLFQLVVTRQVNRFRAETAMTTGALIYAIAIF
                     CVAFGSGFWWFWLCMVAATIGEMILVPTTTTFTASLAPEDMRARYMSLYALTWGIGTG
                     LGLLLAGYVSDVFYSRAMWYAAGLVGFAGFLAFSFIARLKKTNPHGFMHPSAEDVQAR
                     GQRLARGKTRSDCE"
     gene            47955..48392
                     /gene="mscL"
                     /locus_tag="P5516_01775"
     CDS             47955..48392
                     /gene="mscL"
                     /locus_tag="P5516_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large-conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HRT91198.1"
                     /translation="MLEDFKKFIMRGNVMDLAIGVIIGGAFSKIISSLVDDILMPPIG
                     LLLGKVDFSNLFASLTSARYATLAEAQAAGAPTLNYGVFINNVVDFLLISLAIFLLIR
                     AINRLTKKQAEAAPVTRACPRCLSQIPLKASRCPACTSEVTPQ"
     gene            complement(48525..49865)
                     /locus_tag="P5516_01780"
     CDS             complement(48525..49865)
                     /locus_tag="P5516_01780"
                     /inference="COORDINATES: protein motif:HMM:NF013828.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT91199.1"
                     /translation="MPTSSSPDEPSAPSEVCPICGGVGFLHRDLPLEHPEFGKLVPCV
                     CQQNKMIEAARNRLYELSNLAAFKNMSFSSYSVQGRLGLADEQIRSLQYALSQAQQFA
                     AAPRGWLLLMGSYGCGKTHLAAAVANTCVEFGMTTIFLTVPDLLDWLRYSYDATDATF
                     EQRFEEIRNVPLLVLDDLGAHNATQWAAEKLFQIIDYRYTRKLPTVITTNLDLEDLDD
                     RIRSRLQDPDLVTTVHINAPDYRSPVKERRDPLLYQLQLLSDRSFGNFSLREPEQLAP
                     EVQLNLEKVFRAAQEFAENPRGWLVLTGTYGCGKTHLAAAIGNYRKGMGDAPVFVVVP
                     DLLDHLRATFNPTSGTSYDDLFSRVRSAPILILDDLGTQSATPWAREKLYQIFNERYN
                     AKYPTVITMSTTLEDLDARIRSRMLDTRLCSIIEILAPSYRVGVIKTPARKARS"
     gene            complement(49843..50535)
                     /locus_tag="P5516_01785"
     CDS             complement(49843..50535)
                     /locus_tag="P5516_01785"
                     /inference="COORDINATES: protein motif:HMM:TIGR01446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HRT91200.1"
                     /translation="MPEFDGFTAGRNESIPLPATFFTHLLTVIDNLDEMRITLYAFWY
                     LSGQIKEPRYLGLMDILQDNLLMASFGTTTDEQTTRLEEALRRACDRGTLLEARFKDK
                     RFFFLNGEQGKAALVGLKNGTWDPETAQSLPLRLQRERPNIFSLYEQNIGPLTPILSE
                     TLQDAEATYSAEWIEDAIRIAVTRNVRNWQYIEAILRSWKEKGRDGTDRKSAEEKRKR
                     DSEGKYADFIIT"
     gene            complement(50546..51901)
                     /gene="dnaB"
                     /locus_tag="P5516_01790"
     CDS             complement(50546..51901)
                     /gene="dnaB"
                     /locus_tag="P5516_01790"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HRT91201.1"
                     /translation="MSDFDDAATVIQSQPYSREAEEAVLGSVLINPESYYEISEVIKP
                     DDFYIIRNQWIWRSYIRLHERRVPIDILTVSEDLQEQGQLGEIGGQAYLLGLVNQTPS
                     SLHGAAYAQFVEQAAIRRRMLTAANEMARLAYDQEKDIRTILDEVEKSVFSLGASRVR
                     HDLKPVNEVVSEYYDYVDEQSKRDEELSGVPTGLYDLDKLLGGMQKSDLLIVAGRPGT
                     GKTGFLLSVAKTAAQKYKKNVAMFSLEMSNQQLVQRLIAQETGIDTLRLRTGKLQDHE
                     WDVFTKAIDVLSATNIWLDDTPAITPLAMRTKCRRLDNEHKLDLVLVDYLQLMSSDSR
                     NENRVQEVSYISRSLKALARELQVPVLAAAQLSRAVESREEKKPVLSDLRESGSLEQD
                     SDIVIFIHKSKESDKTTNLIVAKHRNGPTSPGIGVVFLEKLAKFVNAAPAESEPAPEK
                     R"
     gene            complement(51923..52543)
                     /locus_tag="P5516_01795"
     CDS             complement(51923..52543)
                     /locus_tag="P5516_01795"
                     /inference="COORDINATES: protein motif:HMM:NF014618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HRT91202.1"
                     /translation="MVEVVIDSVRVSLTNQQRIVMLRESGRERYLPIWIGPYEAEAIT
                     ISLQEIEVARPQTHDLLLQSLTKLGARLIRVEVVSLKKDVFYGNLVLELDGKLYDVDA
                     RPSDSIALAVRAHVPILVNSEILREAGLTPEVDIQAEVLDVEDEPTPEAESDMESEET
                     SKRLSLFEEFLQNVNLDDVAGDSGTQEDDSAAGEGNQGNQDDKPRS"
     gene            52853..53542
                     /locus_tag="P5516_01800"
     CDS             52853..53542
                     /locus_tag="P5516_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012698.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT91203.1"
                     /translation="MSKPRILLIEGKRTDHPSFAAGLTKKGYLVESVPSGAAALAQLD
                     LAVPNLIVVDASSMRTNGKRIVQALRQKQPGLALILVIDSNSDPNDHYDADVILVLPF
                     TMQKLINRIRHLLPGQNNYDLVAGPISLDVTEKRVTIGDRQARLTPRLTILLKILMQH
                     AGEVVERKALFSQAWETDYTVDTRSLDVHMSWLRQAIEENPRRPKLILTLRGVGYRLA
                     VEPITKPRQRK"
     gene            complement(53661..54965)
                     /locus_tag="P5516_01805"
     CDS             complement(53661..54965)
                     /locus_tag="P5516_01805"
                     /inference="COORDINATES: protein motif:HMM:NF024792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tad domain-containing protein"
                     /protein_id="HRT91204.1"
                     /translation="MNRKTEKGQVLVIIALAMIAILGITALAIDGSLVYNSRRADQST
                     ADSAVLAGAGAASQVLKAGNLSQFTCGSTLTSQAATAAVNAARLSAQDDGVDLLPFDL
                     STNNGVDVECGTEAGKTYLKIKAIVSTISEQRFGQVITNQPLRTTVEATSRVSLSGST
                     GGGMGIVSLSPNCGVQGGFDPASKPINVIGGSVFSNSCLYGNGPVTVYGGGTISYIDT
                     DYSSNSTPAPQRATQPVSINIPPPDCSGLLNRGSSTKGGTISPGNYTFIQAPGKTPLI
                     FSPGLYCINSTSLNAFDANAGARVTAQGVTLYIKDGNFNVGSNVQLLMSAPNCSGGST
                     PTGVPPGVCGLLIYMDEPPSNPNQKSTQEMNLVGISGSTFSGTIYVPYGTIDVAGSGT
                     TTTYETQLFGYRIITTGSGKVIFDVSGSNFYSQPGLSIDLMK"
     gene            complement(54997..55437)
                     /locus_tag="P5516_01810"
     CDS             complement(54997..55437)
                     /locus_tag="P5516_01810"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HRT91205.1"
                     /translation="MRIFRYKNRVINSEQGQSLVELAIGILILLILLSGIFDIGRMIF
                     VQFALQDAAEEGIIYASGFPTECNNINNRVRNSSSDLPKDGSVAVSILINNRACGVGT
                     PPKAGDKIEITVTQPFPITMPFLGALTGQTVSLRATANGIVLRP"
BASE COUNT        13306 a        14925 c        14119 g        12973 t
ORIGIN      
        1 gctacgggcc ccaatacccg catcgattat aacattaaga aattgaaccg tgctcgcaac
       61 tcatttcaag gggttctgca cagcggaatc ccctttctca acaattgttg ctttcttcac
      121 catatgctga ggtactcccc tgggccatcc ccgtcccttg acccttcatc aagcagcgtt
      181 tataattcaa tgattgaata ttggagattc atgagttacc catccacctc tggccgagac
      241 ctcgtcatct tagagcaaat cgaacaggat cccgacacca cccaggcagc actcgcttca
      301 agccttgaag tggctgtggg cacggtgaac tggcacctca aacgattgat cgaaaaaggg
      361 tatgtcaagg tcagccgcct ggaacgcaag aaactcaagt acatcatcac gccagaaggc
      421 atcgccctca gggcgcgcct gacagttgac tttattcaaa attcgttcaa tctctaccga
      481 ttggtgcgtg aacgcgctgc gcacgctatc caaaacctga aagcgctggg ttatcaccag
      541 gtacggctgg agggcagcgg tgaagtggct gagatctgcc gtctgacctg cctggagcac
      601 gagatcacgc tggttgaaaa tgagtcagca cctgtgctca aacttgtggg gctgaaagtt
      661 attctggaaa cgaaggagca tgagcatgtc acctgattac tggcaggata aacgcgtgtg
      721 tgtcaccggc ggggccggtt ttcttggttc gttcgttacc gccaggttac acgagcgcgg
      781 agcaaaagag gttttcatcc ccaccattga aaaatatgac ctcgtgcaac tggattcgat
      841 ccgccagatg ctggatgaca gcaacccgga tatcatcatt cacctggctg cccaggtagg
      901 cggtatcggc gccaaccgcg aacacccggc ggagttcttc tacagcaacc tgatgatggg
      961 cgtgcaattg atccacgaag cctggctgcg cggtgttgaa aaatttgtcg ccctgggaac
     1021 catctgcgcc tacccaaaat tcacgcctat cccttttaaa gaggaaaacc tgtgggatgg
     1081 ttaccctgaa gaaaccaatg cgccgtacgg actggcgaag aagatgctgc tggtgcaatc
     1141 acaggcttac cggcagcagt atgggttcaa ttcgattttc atcatgccgg tcaaccttta
     1201 tggcccgcgt gataatttta aacccgagag ctcgcacgtc atccccgcgc tcattaaaaa
     1261 atgcgttgaa gcggttgaaa gcggcgcgga tttcatcgaa gtatggggcg acggcacacc
     1321 cacgcgtgaa ttcctgtatg tagaagacgc agcggaaggc attttactgg caaccgaaaa
     1381 atacgattct cctgaaccga ttaacctggg ctcgggcatg gaaatcagca tcaagcacct
     1441 ggttgaactc atcgctcgtc tcactggttt aaaagggcgc ataatctggg ataccagcaa
     1501 gcctaacggt caaccgcgcc gccgactgga tgtaacccgc gctgaacagg cgttcggctt
     1561 tatagccggg acctcatttg aagaaggcct gaagaaaacc attgagtggt atcgcggtca
     1621 gaaagggtat taaaagcacg aatggcaact gtcaacacgc ttgaacagaa gagaaagccc
     1681 cctgtggtag tcatccagcc atcccgcggt tgggtggggc tcaagctcaa agacctgtgg
     1741 ctctatcgcg aactggtgta ctttctcacc tggcgggata tcaaagtccg ctacaaacag
     1801 tccgtgctcg gcattttgtg ggccattctc aaacctttta tggcgatggt ggtgtttacc
     1861 atcttttttg ggaatttcgc caagatcccg tctgacggtg taccctaccc catcttctcc
     1921 tacacggcta ccttgccctg ggagctcttc gctgccgcgt tgggggttgc cagtcgctcg
     1981 atggtttcaa acagcaatat ggtgtccaag atctattttc cacgcatgat catcccgctg
     2041 gcgtcggtga tgtccagcgt ggtggacttc ttcatcggtt tcactattct cattggtatg
     2101 atgatcttct ataaagtaac ccctactatt gccaccttgt ggctgccgct gctcatcctg
     2161 ctggcgctga ttaccgccct tggtgtgggt ttttggtctt cagcgcttat ggtaaggtac
     2221 agagatgttg gttacattat gcccttcatc gccaatcttt ggatgtacct cacccccgtg
     2281 gtctatcctt ccagtatgat cccggaaaag tggcgactgc tctacgcgct taaccccatg
     2341 accggcgtgg ttgaaggttt tcgttatgcc ctattgggca ccacgcaatc tgtttctggg
     2401 gggatgatac tggtatcctc cctgattgcc atcgccgtgt taattagcgg tatgttctac
     2461 ttccggcgta tggaaaagca attcgccgac atgatttaaa ggacctgtta atgagcaacc
     2521 tggctgtccg cgttgaagat atagggaaac aatacaatat ttttcaggaa accgcccgtt
     2581 acatcacgct tcgtgaaaag atcgtcgaaa ccctcaagac tccggtgaag accctgctta
     2641 accctaccgg cagcgagaag ttttgggcgc tcaggcacgt ggacttcacc cttgaccatg
     2701 ggcaggtgct gggggtcatc ggcaagaacg gcgccggtaa aagcaccctg cttaaaatcc
     2761 tgtcgcgcgt ggtcgaacca actgaaggtt tggctgagat ccacggtcgg gtgggctcgt
     2821 tgcttgaagt aggaactggg tttcaccctg aactcaccgg ccgcgaaaat atcttcctca
     2881 acggcgccat ccttggtatg aagaagtccg aaatcgagag aaaattcgac gagattgttg
     2941 ctttttcaga aatcgagaaa ttcatcgaca cgccggtaaa acgctattcc agcggcatgt
     3001 acatgcgcct ggctttcgct gtttcagccc accttgaacc agagatactg gtggtggatg
     3061 aagtgctcgc ggtgggagat gctgaattcc agcgcaaatg cctgggtaaa atgagcgatg
     3121 tcgcatcaga agggcgaact gtgatttttg tcagccacaa tatgtccgcc attcagcgcc
     3181 tcacacagga gtgcattatc ctggacaaag gcgcgatggt gctgcgcgcg cgcacccccg
     3241 aagcggtgga ttattacctc accaatgatt tttccgccag cggagaacgg cgttacacgg
     3301 aagaagagtc ctcttctgct gcgtatcctt tcaaacctgt cgcggtgcgt gtaaaaaatc
     3361 cctccgggca ggtaacagac agcctgtttt cagtagaccc gctgaccatt gaagtggagt
     3421 atcagctcga cgctgacatc accggtttgc gtatcggcat ctacatgatg acctcgcgcg
     3481 gagaattcat tttcacatcc tttgacaccg atgaaccgga aaaattccaa tccatgagca
     3541 tgcgcaaggc gggtcattac atcagccgct gtgtcatccc cgcagatacc ttcaacgaag
     3601 gacgttttgt gatcggtatc aacgccagct cttatatggt taaacggtat ttcagcgatg
     3661 aacgcgctat gaactttagt atcaacacca ctggcgcacc tggtatgcac tgggtggaaa
     3721 tccgccacgg tcctatccgc cccagacttg actggagaat tgaggaaatc gcgtaaatgg
     3781 cgagcaacaa agagctacta aagaaaatcc tgggggagct tccattgaca gccgaggttt
     3841 accagttgct gcagcaatct gatcgcccgc tgccgaatca tttttcgttg cgcaacatcc
     3901 gaaaggtact tcctgaagca gtggcggctg tacaaaagtc atgcgctcaa taccctgcgg
     3961 gcaagaacgt attcatcttc gcatccaccc attactggct ggagcatggc gtgataaccg
     4021 gattggcgct ttcagggctc ggtcacgatg tgaccatcgg ctattaccct tacggtcgtt
     4081 gggatttcaa ttctccgcag tttgacgtgc ggcgccaaag tgtatattca gccaccgtct
     4141 tgcgtccagc ctccgcggtg atgaatttcg aaaacctgct acacacccat tcttcattct
     4201 cattgctgcc tgaggccctg caacaggctg ttgaccaggt gactgacttc gactacatgt
     4261 acgcgttcca gacagaagaa gtgaataggg aagacccttt ttacctattc aggcatgagc
     4321 gcaatctaca cgcggcgaaa tcgttactca cctacctgcg ttcgttgaag cctgaagttg
     4381 ccatcatccc gaatggcacg atcctcgaat ttggaatcgc atataaggta tgcggttacc
     4441 tgaagattcc caccacaact tacgagttca gcgataaacg ccattcgatg tggctggcgc
     4501 ataaccagga aatcatgcag cagaacactg atgagctgtg gaaaacccac gccaattatc
     4561 aactcaccaa cgctgacctt gaggaattaa aggtactgct ctcttcacgg aaaaacgcct
     4621 ccacttatgg tgatttttct cgccagtggc agcgggttcc cgctgaagga caggcagcca
     4681 ttcaggcgaa actggggttt gatggaaagc ccatcgtcct gttggctacc aacgtcctcg
     4741 gcgacagcct taccctgggg aggaacacct tctccaggtc catgtctgag tggatcattc
     4801 gcaccatcca gtatttcatt gaaaaacctg aagtgcaact ggttatccgc atccatccgg
     4861 gtgaagcctt gatcaagcat ggcagtattc tggaccttat tcgaaaaacg ctgccaacct
     4921 tgccgccgca tattcacctt attgaaccgc tggctgaagt gaacacatac gacctgatgg
     4981 atattgccga tttggggttg gtatacacca ccaccgttgg gctcgaaatg gcgatgcgcg
     5041 gcattcctgt cattgtggca ggtaaaaccc actaccgcaa ccgcggcttc acccatgatc
     5101 ccaacacctg ggtggactac ttcaagctgc tcaacaggct gctgacctgt ttacgtgaat
     5161 gccgcctgca ggaagaccag gtcaacctcg cctggaaata cgcttacctg ttctttttca
     5221 agtatcccct gccgtttccc tggcatatga cgcgcctgaa agaagatctc gaggaatatc
     5281 cattagaata cgtgctttca gagcctggtt tagagaaatt tcgttcaacg cttcaactgc
     5341 tgctctctac aagataaaga ggtagatgat ggatgatcaa gaatatgaaa acctgctttt
     5401 caggcggcag tattttatag gtcctaaaga attagggatt tccggttgga accttactca
     5461 cctgccggat ggcaaaatcc tgagcgctca ccctgaactg gaagttactg attggaatgg
     5521 aaagcacacg cgttacgttc tcatcggcta catttttgat cctttccatc ctgagcgcac
     5581 aaatcaaggc attttagagg caattgaccg ctgcaaaacc gataccgggt caatcttcga
     5641 gatgcttgaa gacaaaagcg ggcattatgt gctattcgtt tacgatgatg aaatgaacat
     5701 tgctctcact gacgcctgcg ggatcaagca aatctcatac tactgtgatg agaatggaaa
     5761 gagttattat tcatcacaac cagccctgat cgcgcaaaaa ctcaacctcc ccgtttcgga
     5821 tgaagccatt cactatatgg actcggcgaa atataagaaa aagctggagc cctggtggcc
     5881 ggtggagtct tcaccttata agagaataag gcgtttggtg ccgaatcact atctgaacct
     5941 tgccacaaac gagcaagttc gtttctggcc gaataagaag ctgaagcgtt attctctgaa
     6001 agaaggcacg atcctggctg gggagctatt aaaaggcatc tgtaaagcct gttttcaacg
     6061 ctaccccatg gcattaacct ttaccggagg atacgactcc cgggttctct tagccgcctg
     6121 taaagaattc gctgatcaga ttgaactttt ttctatgatt taccgcaacc tcacagaaca
     6181 aagtgaagac ctgcggattc cccaggcaat cgcggcagaa aaacaactca agtatcatca
     6241 aattaaatgc gacgcggaaa taccagccgg gtttcaagat gtctatgagc gcagtaatca
     6301 agggtataaa actgattgga tgagccttgt gtatggcagg tatatccatt ttcctgccaa
     6361 tgtattgatc gtcaaaggca acattgcaga agtggctcgt tgttctttct ggcaggatgg
     6421 catttaccct gtagaagtaa ctgtggatac gcttgtggat gctaccgcct taggacacga
     6481 accccttatt ttacaatcca tgggaaaatg gttggatgaa gccaaaccca ccgaaaagtt
     6541 cggatataaa atcatggata ttttcgcctg ggaggtggag gcaggggggt ggcagtctat
     6601 gagccacaat gtttttgctt tgtcgcagga agaattttct ccatttggca accggcgcct
     6661 tcttgatatt atgttaggcg tgcacaagcg ctatcgctgc tggcccgata ttaccctcga
     6721 acaggaaatc atcaaatacc tctggcgtga cctttcacaa tactcctatt tctcttcgtg
     6781 gaatttgcat aattacaaaa agaagtttta tgatggcgac ctgttgaatt tcctgcgcaa
     6841 gattaaactt atactgaaga ggagcattaa atgaattggt ctgacagtgt cgttctggta
     6901 accggaggga ccggttcgtt cggtaagaaa tttatcgata tcatgctcaa ggaatatcat
     6961 ccacgcaggc tggtcgtttt cagccgggat gaattgaagc aatttgaaat gcaggcggcc
     7021 gggttttcac acccttcgct gcgttacttc attggagata tccgcgacct gcagcgttgc
     7081 cggcgcgcct ttgagggtgt ggacctggtg gtgcacgctg ccgcgctcaa acaggtgccc
     7141 gcctgcgaat acaaccccat ggaagccatt aagaccaata tccttgggag cagtaatgtc
     7201 atcgatgccg ccatcgacgc tggagtcagc cgggttgtgg cgttgagcac ggacaaagcc
     7261 gtcaacccca tcaaccttta cggcgcgacc aaactggctg cggaaaaatt atttgtgcaa
     7321 tccaatgcat atgctggtgc cagaaaaaca cgttttgcct gtgtgcgtta tggaaatgtc
     7381 gtcggcagcc gcggctcggt ggtaccgttt ttcctcaaac aacgcgagac tggcagggtg
     7441 acggtcaccg atgaacgtat gacgcgcttt tggatcagcc tggagcaggg tgtgcgattt
     7501 gtcatccgcg cggttgaaaa catgcacggc ggggaagtgt ttgttcccaa gatccccagc
     7561 atgacggtca ccgacctggc tcgtgccatc gctcccgagg ctgcgattga tttcattggt
     7621 atccgcccgg gtgagaaact gcatgaagtt ctcatctcag aagatgaagc gcgccagacg
     7681 gttgaactgg aagatatgtt cgtggtgcaa ccggcggagt ctctctggtt cggcagggat
     7741 tgggagggca aaggacgtat gctggaagat ggcttccgat actcttccaa taacaacgat
     7801 cagtggttga acgtggaaca aattcaaagc attatcgccc ccatcgaagc cgattacctg
     7861 gcaggcaggc tgggatgacg cgcttcctgg ttaccggagc aagcggttta ttagggctga
     7921 attttgccct ggcggtggat ggcaaaaaac accaggtcac cggtgttgcc aacacagccc
     7981 cgatgttgtg ggcaactttt aaaaacgtcc agctcgagct cacccaaccc ggtgtggtgg
     8041 atgagatgat aaaggagcat caacctgaag tagtgattca ttgcgccgcc atggcgaatg
     8101 tggacgcctg tgaaaataac cccgcccagg cacggctggt gaacgctgaa ctacccggca
     8161 cgatcgctgc agcctgtaaa cgccacaggg tgcgcctggt tcacatttcc accgacgcgg
     8221 tttttgacgg taagaaaggg gactatagag aagaagaacc cccccatccg ctgagcgttt
     8281 acgccagcac caagctggag ggtgaacgag cggtactcgc tgccaatccc gatgcgctcg
     8341 tggcgcgcgt caacttctac ggttggagcg ccgcgggcaa tcgcagcctg gcagagaatt
     8401 tcgtgcgcaa cctggaagca ggcaaggaaa tgatgggctt tacagatgtc atcttctgcc
     8461 caatgaatat cctcgatctc agcagtgtgc tggtggaggc tgccgaactg gatatcaggg
     8521 gtgtatatca catggttggc gcgcaggcga tgagcaaatt ccagtttggc gtggttctgg
     8581 cagagcaatt cggctttgac accaggctca tcaaacctgt ctctgtaaat gacagcggtc
     8641 ttaaagcagc gcgttcgccc aacctgagcc tctcaacgga aaaaatcgta aaagccctgg
     8701 ggcatgaatt accagatttt cacgaaggcg tgaagaaatt ttttgaccag taccgccgcg
     8761 ggtatccgca atacatcatg tcactgaacg gctaacaggt aggcgaaagg agcaaaaatg
     8821 gaattgaaaa ttggtcagca ttttatcggg ttgcagcacc ctacctactt catcgcagac
     8881 atttcagcca accacgatgg cagcctggag cgagccaaga tgctcatcag gctcgccaaa
     8941 gaagctggcg ctgatgctgc caagttccag aacttccgcg ccccaaaaat cgtgtcagat
     9001 tacggcttta aagccatgca ggggcaggtc tctcatcagg ctacctggaa gaaatccgtt
     9061 ttcgaagtct actcagacgc ctccatccct tttgactgga ctcccatcct caaagaggag
     9121 tgcgataagt taaatattga ttacttctcc tctccctatg attttgaagc ggttgattat
     9181 ctcgacccgt tcgtgccggc gcacaaaatt ggttccggcg acattgactg gctggaaatg
     9241 ctggaacaca ttgccctcaa aggaaaacct gtcattcttt ccagcggtgc cgccaccatc
     9301 ggcgaggtgc agcaagcggt acacaccatc ttaccgctta accagcagtt ggtcttgctg
     9361 caatgcaata ccaattacac tgccagcccg gtgaattttg accatataca tttgcgcgta
     9421 ttacaaacct atcagacgct cttcccggaa attcctgtcg ggctgtcaga ccacacgcac
     9481 ggtcacgcta ctgtcctcgg cgcagtggcg ctgggggcga gggtgatcga aaaacatttc
     9541 actgatgaca ccagccgtgt agggcctgat caccccttct cgatgactcc cgttacctgg
     9601 cgtgagatgg tggatcgcac acgcgaactg gaacgcgcgc ttggctcagc tgataaatcg
     9661 gtcagcggta acgaggtaga cactaccatt gtgcagcgcc gctgcctgcg cgcctctcgt
     9721 gatattcgct ccggtgaggt tttcacccgc gagatgatcg atgtgctgcg cccggctact
     9781 cccggcgcca tccgccctga ccaggttttt caggtggtcg gaacccgcgc actcaaagac
     9841 attcccatgg gaaaagagtt gcgctggacg gatttgggag agtaatttga aagttgttta
     9901 tttcacccgc gattattcac ctcatgacga acgctttctc accgcgctcg cgcaaacgca
     9961 acacgaggtg tatttcctgc ggttgtcgcc ctcaagcgaa gtccatttac cacccggaat
    10021 acgcgaagtg aaatgggtcg gccccctcgc cggtgaaata atttctgatg acaggcgcgc
    10081 ggaagatctg aatcagcttc tttccaggct gcagcctgac gtgctgcacg ccggtccact
    10141 gcacgggccg gcatatatcg ccggtttaag tggctttacc ccgctggtga gcatgtcctg
    10201 gggttcagac atcctgcatg atggagaagt tgacgccctc gcccgggcaa aaatcaacct
    10261 ttcactgcat cgttccacgg ttttcctggg cgactgtcag gcggtggtag aaaaagcgat
    10321 caacgctttc gcgtttcccg cggagcgggt attccggttc ccgtgggggg tagacctggc
    10381 gcacttttca ccctcaacaa ccagcccgct gcgcgcatca ttaggctggc agcagcaatt
    10441 cgtcttcctc agcaaccgct ccttcgaaga gatatatggt gtagacgtgg tcatgcgcgc
    10501 cttcatccag gcgcaatctc gcgccccaaa tatccgcctg ttattgtatg ggaagggcag
    10561 ccttcaacaa gaaatcatgc gcatggctga aaacgcggga gtgatgcaca agatccattt
    10621 cgctgccttt gccgggcatg aagatctgcc agccagttac cacgccgcgg atgtattcct
    10681 cagcgcgtct cattgtgacg gttcctctgt ttcactgatg gaagcgttgg cctgcggcaa
    10741 acccgcgctg gtttccaaca tccccggtaa cctcgaatgg gtgcaccacg gcgaaaatgg
    10801 ctggcttttt caagacggtg atactacagg gctggcagac gccatggtag cggtcagccg
    10861 caagccgaat cttgaaaagt tgggtcaaaa atcccgggcg ctggccgaag aaaaagctga
    10921 ctggtcgtgt aatttcgccg tgttattgca ggcttaccaa aaagccgtgg aatggcgtac
    10981 gcccgccctg gagattacgg agcgggtgaa atgaaagtgg tggccatcat ccaggcgcgc
    11041 atgagttcca cccgcctgcc cggcaaagtt ttaaagctgg cttcaggtcg cacaatgctg
    11101 gaccgcatgc ttgaacgcgt taaaaaagcg cgaacgatca gcgaggtggt aacagccacc
    11161 acaactgatc cttccgatga tagtattgct cgcgcttgta aaaaactggg ggtgagtgtg
    11221 ttccgcggca gcctgcagga tgtgctcgat cggttttacc aggcatcgcg catgcatcaa
    11281 gcagatatca tcgtgcgcct caccggggat tgcccgttga tcgaccccgc gctcatcgat
    11341 gacgtggtga acgctctcac ccggaaaaag gtcgattttg cctgcaaccg tcttccgccg
    11401 cccttttcac gcacctaccc catcggactg gatgtggaag cctgtacttt caacgcgctg
    11461 gagcaggcct ggtatggagc gaaagagaag ttcgaacgcg agcatgtgct gccttacctt
    11521 tacgctgtgc ctggcaggtt ttccgtgctt caattgaatc acaccacaga cctcggtcac
    11581 ttgcgctgga cattagatac gcccgaagac ctggtactgc tgaggcgtat ctaccggtat
    11641 tttcaaggcc gcaatgactt ttcatggttg caggtcttac aattacagca gcaaaaaccc
    11701 agcctgttcg ccatcaacgc gcaggtgcgc cacaaaacct acctggaaaa tgaatccccg
    11761 agatgaccat taaagaaatg tcagaaaaaa tcgtctgtcc ccgatgccaa tcctcttttg
    11821 ataagcacga tcctgctgtt tggagctgct cttcctgcgc ttacacagtg ccattatttc
    11881 aaggaaagcc ggtgttctcc gggcatccgc aagagcagca gccgcaagac aagatagagc
    11941 gcggcgcaga caaaggcaca gcatggcgcc gcgccaattg gcgctttatt catgctattg
    12001 cggcatctct tcccgcaggg gctgacgtgc tggatgttgg cgccggtcgc ggtgattttc
    12061 gacaaatctt cgcccatcac tcatacatcg ggttggatat ttacccttac ccggaaattg
    12121 acctggcggt ggatctgatc gagattaacc cattcaaaga acgctcgttc gacctggttg
    12181 tgttggcgaa tgtcattgag catgtatacg aatatcgcaa actgatcagc cgctgcgcgt
    12241 tcctgctcaa gcccggtggt cagttgctga taacagtgcc cttcctgctt aagctgcacc
    12301 aggaaccggt ggattaccat cgttacaccc actatgcctt attccagtta gcagaagaaa
    12361 accaactcag tgtggcgcgt tttgaggttt ataccaatcc attcgcgctg ctggacgaag
    12421 ggattgctga tgtgtgggag aacgtgcttc cgctgcgcca aagaattgac cacctgcaca
    12481 ccaaggtgaa agtagcgctt attcaaaagc tggcgaacag catgaaagcc aatcaggcgg
    12541 atggtgtggt gaaacccgtc caagagaaca gcaatccccg ggtgctgggg tatatgtgtc
    12601 tgtttcaaaa ggatgccctt caatgacaag cgtggctttt ttcaccgctc ccaaaccatt
    12661 cacaaacgcg catatccgca tcatccagcg caacgccatt caaagctgga aatcactggg
    12721 caggcaggtc gaagtgtggc tggtggggga tgaaccaggt gtggagcaga acgcgcgcga
    12781 gttgcaggtc ggttatatcc cgggagttgc gaggaacagc ttcggcacgc cgcgcatcga
    12841 ttccatcttt cacctggttc gtgaacaatc gcaggcagaa tacctgtgct atgtcaacgc
    12901 ggatatcctg gttttcccgg atatgttacg cgctctcgaa gaggtgcgcg cgcgcgtcga
    12961 tcgtttcctc atcatcggca ggcgctggga cgccgcagtg acagaaacac tcgacataaa
    13021 agaaggctgg catcaacagt tccaggctgc cgcgctagtg accgcaaaac tgcaccgcgc
    13081 cgcgggcagt gattattttt tattcccgcg caacgaattc atcgatatcc cgggtttcgc
    13141 ggttgggcgc gccggttggg acaactggat gatctacaaa gcccgatacg aacatatggc
    13201 agtggtcgat gccactggcg ccatcaaagt catccaccag aatcatgatt tcagccattt
    13261 tgccgacggc aagattcacc gccttcagcc ggaatccgct gaaaatatgc ggctggcagg
    13321 cggtcgttac acggtcttca ccattcacga cgccaattac cagttagaca acgacggtct
    13381 gcaacgcgta cgcctaaatc gctggaaatt gctgcgagaa ttcgtcattt tcccagccgt
    13441 tacgctgcac tgctcctggc tggcgaaagt cgtttatgcc atcttcaacc cgaaacgttt
    13501 gcttaaagac aggcgccagg caaaagaagc gctgcgccgc tcaaaggaag ggtaaatgag
    13561 aaaaggtcaa aacccggtaa aacaacttca gacggtgtct aaaccgcagc gggtgaccgt
    13621 ggcggtgttg aaccacatcc cttttctgga tggcttttac gcgcagatgc tcgatgtttt
    13681 acagctcagc ttgagcacac tgcgccagaa cgcaggcttg gattttgacc tgcttgttta
    13741 cgataacggc tcttgcaagc aggctcgcga ctttttactc gcagaatacg aaaaaggaaa
    13801 tatccagtca ctgatcctct cagaccgcaa catgggcaag ggcggcgcct ggaacgtgat
    13861 gctgcaagcg gcgccgggtg agatcatcgc ttacgcagac aacgacgttt tctatcatcc
    13921 cgggtggctg cgcgaatctg tgaaagtgct cgaaacttat cccaatgtgg gtatggtcac
    13981 ctcgcggccg tttcatacca aaccagaatt gtacagcgct gacctcgatt gggcgcgtaa
    14041 aacacctgaa gcgtcagtag aaagaggttt ttttatcgac cgtgactgga tgcgcgagtt
    14101 ctggctcagc gtaggtcgca gcgaggagga gatcgcccta gaccaggcgg ttgaagatgt
    14161 gcgcctcacg tataaaggtc ttgtcaccta ctgcggggct tcccactggc aattcctggc
    14221 ctggaagaag gtccttcagg aatttcttcc tattgatcta tcgcgcccgc tggggcaggt
    14281 tcttaagctg gatgagatga ttaaccagca aggatacctg aggttgatga ccgcccagcc
    14341 gttcaccatg aacttgagca attcagtcga actgccctcg aatgaaccga caagcgcagc
    14401 agctcaaccc cgtacgcgca aacggctggc agattttcct gtggtcaaat ccccgctgat
    14461 gcggttgtac aacgccatat tcaagcttta ttatgacgat aagaagcgtt gaggagaaaa
    14521 aattgaaaat cttatacgcg acgggaagat acagcccact caaacacgac gatggcagcg
    14581 gtacagatta ccggctgtat cacgcgctat tgagccaggg cgtagagatg aaattcatcg
    14641 gcccattccc tgatcacccg tcgctgatag agcgccttta ccgcaaggtg cacaccgtgt
    14701 tttcgcataa gcgaccggcg aaatataccg ccgcaatgct caaaacagcc gcgcaaacat
    14761 tatctatcac tgttaatgaa taccaaccag acgtgatctt ttcaaaaaac cttgccccac
    14821 tcgtgcgttt ccatgcccaa ctcccagtgg tgtacatgct ggattccact gtggcggcat
    14881 ttaaccgagg ctggccaact ttttctcgtt tggaaaactg gcgcatggtt gaatgggaaa
    14941 agaaagtgct taagcaatcc agcagcgtca tcacccgcag tgagtggaca aggcaatcgc
    15001 taatagaaga ttatcactat ccggctgaaa gaattcatat cgttcacaat ggctcttccc
    15061 ttcctgatca tgttattcca caagagattg agaacgaacc gcccgatttt tccaccatac
    15121 gattgctctt tgtcggacgg gtgatgcatc tgaaaggaat cgatatcgcg attgaaatcg
    15181 tcaaccagtt gaacgaatgt ggcaggcagg ctgacctcag aattgtcggg cttgatgggc
    15241 aggaccaaga ccacatccac tacatggggt catttaaaaa aagcattgaa gatcagttaa
    15301 tcgcctacgc aaaccagtac cgatggccgc atttcctttt acatcccgcc cggtatgatg
    15361 cggcccccat tgttaccgca gaagctgccg cgtttggcgt gcctaccctt accaataatg
    15421 tggggggtat cgcctccatg gttcaagatg gtctgtctgg ggtcgtgctc cctgctttaa
    15481 gtccggccgc agaatatgta aagaaaattg tgtattacgc tgaccacccg caggaataca
    15541 tggcactgcg ccgcactacg cgcgaacgat acgaaaaaga actcaactgg aatgtagctg
    15601 gtaaaaagat agttaccatc cttcaaagtg tggtggaaga aaatcagttg aaacccgggc
    15661 tgaagaaaga gcaaccatga aaatcttaat tgcgggttat ggttcgattg gcagacgcca
    15721 cctcaacaat ctgcgcgcgc tgggtcaaac tgacctttta ttgctccgct ctcaccgctc
    15781 cacgctctcc gacgttgata tacaggatgt gcccgtggaa accaccatcg aagccgcgtt
    15841 ggcgcaccag ccgcgggcgg tggtcatcgc caaccccact gccctgcacc tggatgtagc
    15901 cattccagcg gcgcaggctg gctgttccat cttgatggaa aaacctgttt cgcattcaat
    15961 gagccgggta gaggaacttc gccgcgcgct cactagcggg ggcggggttc tgctcagcgc
    16021 ttttcaatac cgtttccacc ccgggctgcg ccaggtcaaa cgctggctgc aagaagacca
    16081 gatcggcgtc atctcctcgg tcaagtcgca ttggggcgag tacctgccag gctggcaccc
    16141 ttgggaagat tatcgcagca gttacagcgc ccgggtggac cttggcggtg gagtggtaaa
    16201 caccctgtgc cacccgtttg actaccttcg atggatgttc ggcgaagcag aacagttagt
    16261 tgcttctacc tccagccggg ggttgaacct gccggttgaa gacacagcag atgtgctgct
    16321 tcgctaccag ggtggtgttt gtgccaatat acatttagat tatttgcaga gacccggtca
    16381 acatgacctg cttatcaccg gcagcgccgg caccatacgt tggtcaaaca cggatggcag
    16441 cgcgaaaaga tatgacccca tccatgacca gtgggaagag gtgcgcccgc cagaaggttt
    16501 tgagcgcaac accctctttt tagaagaaat ggcgcatttc ctgcgcgtgg tggaaaggca
    16561 ggaagcacca ctgtgtacac tggaagacgg aattgccgtg ctacaactaa cggaagcagt
    16621 gcaccgctct gcccgtgaag gatgtctggt aaaattttag cggcctattg gaggttgaat
    16681 gaccagtatt tttgaaaaat tttcattgaa aggcagggtt gcgctggtga ctggcggcgc
    16741 cggcctttta ggaaaagagt tctgccttac cctcgcccag gctggcgcgc aggtgtacgt
    16801 cgcagacctc aatctgagcg cggctgaaag cgcggcgcaa accatcaccg cccaaggtca
    16861 ttctgcctgc agcatcaggc tggacgtgac caacccggat tcagtgcagt ctgtggtcga
    16921 tgaaatcgtt tcccgttccg gcagtattga tgtgctggtg aacagcgccg caatggaccc
    16981 caagttcgac cctggtcacg cgcaaaccaa cagcaacgct tttgaagcat atcctctggg
    17041 gtcatggcaa gatgcgctca atgtcaacct caccgggcca ttcctggttt ctcaggcagt
    17101 ttgccgccaa atggtgcggc agggtaaagg ttctatcatc catatttgtt caacatacgg
    17161 gttgggcggg cctgatcagc gtatctatga aaaacccgga cagtcaaaag tgttcaagcc
    17221 agtgtattac accgtcacaa aagccgggat cctgggtctt acaaaataca tggccaccta
    17281 ttacgcggga agtgaaatcc gcgtgaacgc actcactcct ggaggggtct tcgccgggca
    17341 cgatgacgtt ttcgtgaaga actactcggc gcgcacggtc attggcagaa tggcccacaa
    17401 agacgagatg aatggcgcgc tgctcttttt agcctctgac gcctcctctt atatgacggg
    17461 tggaaacgtg gtggtggatg gaggatggac agcttggtaa aaaaaccgga ggtattagcg
    17521 cttattcctg cgcgcggcgg ctcaaaaggc atcccgcgca agaacatcaa actgttcgcg
    17581 ggttttcccc ttatcagtta cagcattgct gctggccggc aggcgcagag cgtcacacgg
    17641 gtcattgtgt ctaccgacga tgaagaaatt gctgctgaag cgcgccgttg ggaagccgaa
    17701 acgccctttt tgcgcccgcc cgaactggcg caagatcaaa ccactgacct gcctgtcttt
    17761 cagcacgcgc tgcgctggct tcgtgaaaac gaaggctata ctcccgacct ggtcatccaa
    17821 ttgcgtccca cctcacctat ccgtccgcgt ctttgtgtgg atgaggctgt ggcgcttttg
    17881 agaaatcatc caggcgcaga tagtctgcgc ggagtggtgc ccagcggaca aaacccctat
    17941 aaaatgtggc ggttgcaggg caaagacgaa ccgatgaccc ccttgctgaa actggatgga
    18001 atcgctgaac catttaacgc gccccgccaa tcgctgccgc ctacttactg gcaaaccggg
    18061 catattgatg tgatccgccc tcgggtcatc cttgagggca attcgatgag cggcagaaat
    18121 attctgccct acctcattga ccctcggtac acggtggata tcgatactcc ctttgactgg
    18181 ctgcgttacg aatggctggt gcgcaacgct gacctggata tggttgaccc ggccagcaag
    18241 cggcgcaaac tccccgcgaa gatctcgctg gtggcaatgg attttgacgg tgtgctcacc
    18301 gataacctgg tctatgtgga tcaggatggc agcgagcgcg tcgcttcatc gcgcggtgat
    18361 agtatgggca tccgcctggt gcgcgaagcc ctgccggtaa attttgtggt catctccacc
    18421 gagaccaacc cggtggtcag cgcgcgctgc cgcaagctaa acctggaggt ctaccagggg
    18481 gtcaatgaca aacccgcggt cctcaaacag ctgctgcaag aacgccaggt tcctgctggg
    18541 gaggtcgtct tcatcggcaa tgatgtaaat gatgtcggtt gctttgacct tgctgcttac
    18601 gcggtcgccc cggctgacgc cgagccagaa gttcgacgcc gcgccgacct tgtgctcacc
    18661 cgtaaaggcg gaaaaggcgc ggtgcgggag ttttgtgacc ttcttttaca gaatgctggg
    18721 aaaattttgc ctacacagga gaaaaaatga cacgagaagt caagattggc aataaatggg
    18781 tcggcgacgg ccaccccgtc tacatcgtgg ctgaagttgg catcaatcat aacggtgata
    18841 ttgaaatcgc caaagaactt atccgcgcgg ccaagcacgc cggcgtggat gctgtaaaat
    18901 tccaaaaacg tacccctgaa ctatgcgttc cacctgaaca acgcggacag atgcgtgaaa
    18961 ccccttgggg ctacatctct tacctggatt accgctacaa agtggaattc aacgaggctg
    19021 attatgatga aatcaaccgc tacgcgcgtg aaattggcat cgaatggttc gcgtccgttt
    19081 gggatatccc ttccgttgac ttcctggaaa agtacgagcc catctgttac aaaatacctt
    19141 ccgcgtcgct gaccgacgtg gaactgctgc tgcatctcag aaagaccagt cgtccgctca
    19201 ttctctccac gggtatgtct acccaacaac agatcaaaga cgcggttaag cggataggcg
    19261 aagaaaacct ggtcatcact cacgccacca gcgcctaccc ttgcgacccg tatgaattaa
    19321 acctccggat gatccaaacg ctgcgcggta tcttcaattg ccctatcggc tactctggtc
    19381 acgaagtcgg actggtgacc tctgcagtgg cggtgggttt gggggcctgt atggttgagc
    19441 gccacatcac gcttgaccgt gcgctgtggg gatctgacca ggcggcatca gtggagccgg
    19501 gcggttttga aaaactggtc aagtatatcc gtgtcaccga agcagccata ggcgacggcg
    19561 tgaaaaaggt gtacgaaagt gagatgccct cgctgcgcaa gttaaggcgg atccaggatt
    19621 gaaaaaagcc tttttgcggg tgagaaacgg tatcaggcag cgctgggtgc ataaaaaagc
    19681 caaccagcgc tggcgggctt tagccacagc catcaccctg caatcgccac tgcccagcga
    19741 cccgcgacca gtgatatttt ttaacgcttc cacccggctg caggcgatga gccaaaatgc
    19801 cgcttatgcg ctgctcaccg cgcaggcgtt gcgcctgcag ggtgttcctg tcatccagtt
    19861 cgtgtgcggc ggggcgttgc agcgttgtgt gttgggcagc cagcaggata atttcaacct
    19921 gccgccgcca tgccaggcct gcacacgcca aagccaggca gttttccagg agttgaatac
    19981 ccgttggatg gaaacgcgtg aacaccccat gcttggaaaa gagctcgaaa ccctatcctt
    20041 caatgagtat gaaaccttca cctatcaagg aactccgctg ggtgcctggg cggtcaattc
    20101 tctgcgttgg atgatgcgcc gccatcacct gcaggaggat gaaacgacgc gcgctttctt
    20161 ccgctctttt atcctctcca gttggaatgt attcactcag ttctctgagt tactggatga
    20221 aacccaaccc cgggcggtgg tgctgtttaa tggcatgttt tttccagaag ccgcggcgcg
    20281 ccacgcctgc ctggaacggg gggtgcgcgt gatcactcat gaggtcgggc tcaggccctt
    20341 tacggctttc ttcaccaccg gcgaagccac ggcttacccg atgatcatcg accccgcctt
    20401 tcagctcgat gatcagatgg agaagaaact cgataggtat ctctctacac gttttcgcgg
    20461 tgagttcacc atggcaggca ttcggttctg gcctgaaatg aaagcccttg aagctgattt
    20521 actcgaaaaa atcaatcact attccaaact cgtggcggtg ttcaccaatg tgattttcga
    20581 taccagccag gtgcacgcca ataccctttt tgagcatatg ttcacctggc ttgagcatgt
    20641 aaaaacaact gctctttccc atcctgaggt gctgttcgtc attcgcgctc accctgacga
    20701 atgccgacag ggaaaagaaa gccgtgaaag cgttgccacg tggttctctt catctgaaat
    20761 ttcccgtctc gataatgtgg tgttcattgg ggccgatgag tacctcaatt catacgacct
    20821 gatccgccgc gcgcatctgg tgatggttta caactcaacc atcgggctgg aagccgcgct
    20881 gctgggaaaa cctgtgctcg ccgggggcaa ggcgcgcttc acacagcttg aaaccgcctt
    20941 ctacccgcag tctgcgcagg aatacaccgt gatgcttgag cggcttctgg caacgccgag
    21001 cattgacaca ccacctgagt tcgcccgtaa tgcccggcgc tttttgtatt accagcttta
    21061 cgccacttcc cttgacttca gtgactacct cgaggaagac ggcgtgtgga aaggttacgt
    21121 taaactccgc gagttctcac ctgacctgct taagccagac aattctctta caatgaaggt
    21181 gctgttaaat ggcatccttg agaatggaga ttttactctg ccgctatgac cactccagtt
    21241 tgttcgatta tcatacgcgc ctataacgag gaaaaacata tcggccgcct gctgcgggga
    21301 atccaggagc aggacctcca tgatgtgcag gtgatcctgg tggattcagg ctcgacggat
    21361 ggtactcgcg acgttgcccg cgcctacccg gtagagatcg tagagattga cccgcgtgaa
    21421 tttactttcg gtcgttcact caacctgggc atcgccgccg ccagcgcgcc cctcatcgtg
    21481 atggccagtg cccatgtcta tccagtctac cccgattggt tgacaaaaat gctgcagcca
    21541 ttccagcaca ctgaagttgg cttggtttat ggcaagcaac gcggcacagg cacaacggct
    21601 ttctctgagc atcaaatctt cagccactgg ttccctgatg tctcagtcaa gaaacaatcg
    21661 catcctttct gcaataatgc caacgcggct attcgaaaag agttatggca acagcacccg
    21721 tatgatgaaa ccctcccagg gttggaagac ctggagtggg gaaaatgggc gcaggagcag
    21781 ggttatacca tctcttacgt cgccgaggct gaaatcatcc atgtgcacaa cgagtcgctc
    21841 gacggtattt acaatcgtta taaacgcgaa gcgatggcgt ttaaacgtat ctacccgcac
    21901 gaaactttca cgctgcgcga tttgaaccgc ctttttttac aaaatgtaat cagcgactgg
    21961 cgcgaagccc ggaggcaaaa ggtcttcgct caccattttt tccatatcat cggattccgc
    22021 tggcggcagt tctacggaac ctaccagggg taccgccagt ctggcccgct cacctggcag
    22081 ttgaagaggg cgttttacta cccccgagat ttccgccagg catccgggca aaccagccgc
    22141 cgagatatcg agccaattaa gtataattag gcaggttgat agaaggtact tgcaggagga
    22201 taaaatggcc catgtaacta ttgagtttac cggtctttca aagtcaatta caggggtaaa
    22261 cgaggtaaat ttgccgatca accccacaga tacctaccgc gacgtggtac gccagctggg
    22321 aacgctttat cccgcattgg tagggatact catcgctgaa gatggcgaga ctttcctgag
    22381 ttccaacatg ttcatcatcg atggcgatta cgccaatccc gctatggtga tggagaactc
    22441 tcccagggac ggtgaacaca ttcacctcat gtctgtcatc accggtggct gaaagaacca
    22501 agaaagtcga ggtttgggtg cctgagaaac tgcaacaccc caagatctcc gctctggtgc
    22561 cgatgcggca tcactctcaa cgtgtgcctg ggaaaaacta ccgccctttg aacggcattc
    22621 ctctctacca gcacatcctt accaccttaa cgcaggtgcc agaagtggat gaaattgtcg
    22681 tggatactga cagcgacatt attctgcacg ggatccagtc aaacttccct gctgtcaagg
    22741 tgatcaatcg cccgccagaa ctacgagcgg atgatatttc gatgaacacc atcctgttgc
    22801 acgatatcag ccagtcacca gccgacctct acctgcaaac gcattccacc aacccgctgc
    22861 tcaaagcctc cacaatctcg cgggcgatca acaagctctt acaggtctac ccggaacacg
    22921 actcgttgtt ttcagtaacc cgcctgcaaa cccggttgta cgaccatcta gggaaggcga
    22981 ttaatcacga ccccaaagtg ctcatccaaa cacaagattt accgccagtt tacgaggaaa
    23041 attcctgtat ctatctgttt accgctgcca ccctgcgtaa gaacggcaca cgtatcggag
    23101 agacccctct actgtttgag acagatccag aagaatcctg ggatatcgac gaagaggttg
    23161 attttaaatt agtcgaaatg atactgaaag cgcgctaacg aatgaaacgc ttttcctcgt
    23221 tttcagtgtt gttgcagctg gggatcttcc tcctgggggt aagcggtagc atttacgtgg
    23281 cttttactcc cgcaaacagc atgatgaact ggtacagtaa tgatgatgcc ttttattact
    23341 ataaagttgc gcaaaatgtg ctcagcgggc atggtttcag ttttgacggt atcaacctta
    23401 ccaacgggtt tcacccgttg tggatggtca tttgcctggg cgttttctgg ctctccagat
    23461 tccatctgct actgccgcta agggtattaa tcgtcatcag cggggttctc aacgggctca
    23521 ccggcgttgt gttgttgcgc ttgcttcaaa agtttttacc gcttgtcgct gccatattag
    23581 gcgcatgcgt ttggattctc ctcccttcga tttacaataa ttacactgcc cacggccttg
    23641 aatcagcgtt gagcgctttc ttcgttgctg tgttgttgct aaaaagcgct gacttgctga
    23701 ccggtcccgc agaaaaacgc gccggaaaat tgatcatact gggtatcatt gccgcgctca
    23761 ccatcctcag ccgcctggac acgcttttcg tggtcgccat ggtggggttt tttgtggttt
    23821 ttaagatnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23881 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnttt tttaccgacc
    23941 ttccagccag gtgctcagtg tgtctgcctg gcagcaagta aaaaattgga tccggcaagg
    24001 actgccccat cacctccgtg acggagtgct cttctccttc cccattgtgc tgctggtctg
    24061 cggttacatg ttaaccaata agctggaatt tggtaccttt ataccggtga gtggccaggt
    24121 caagacctgg tggggaacgc tggaaaacac cgtctaccgt cagaacacga ccctggtcgg
    24181 attgatgggt ttggcgccag gcagagggaa aagcccatgg tcgctgctga cctcgatcgt
    24241 tgcggatatt tcgattttca tcaggaacct gttcgtaaaa gatacggaag acctgccggt
    24301 catcctgttt cttgttttcc ttttcatgct gttcattttg ctgatctttt tgctttcccg
    24361 caaggatggt ctgctggcca gacgctcctt cgcccttctg attcccgcgt tggcaggcgg
    24421 ctgtcttttg cacattgctt attatgccgc gcgcaattac gggcatacgc gcagctggta
    24481 ctgggtgcct gagacgatgc tcctcgtgct gttgggagcc gtggtgagct ccaggatgtt
    24541 tgaaaaaacc aggcagtgga cacgaacacc cgtggctgaa aacctgctgc tgctggtaat
    24601 ggttggcttt gtctttttct tacacgcccg atatatcgcc aggctctgcc cgccttcggt
    24661 ttctccggag caccaggcag actacctggc agagacccgt gaattagaat cctatacccg
    24721 cgaaaactcc ttgattggta tgacaggtgg tggagcaacc gcgtatttca ttcaaaatcg
    24781 caccatcatc aatctggatg gcttgattaa cagtgccgaa tacttcagca cattaaaaac
    24841 cggagccacc aaccgctttc taaataacct gcatctggat tatgttttcg gtaattcgta
    24901 catgctactc aaaacagatc cttaccagca aatttttacc gggcgtataa gggaaatcgg
    24961 caacattcac agcgggtcgc acttcacgtt atatgattac atcggcgacc agtgaggtcg
    25021 ccgcgttgag aatatatttt gaaggttata ataaccatta ctctatttaa ttgaaagaaa
    25081 acccttatgc cagtcctctt attatctgcc ccttatatgc ttcctttcgt caatcgcttt
    25141 aaaccggttt ttgatcatta ccagctggaa ctgctcctgg cccaggtgaa tgagcgcctc
    25201 ggcgaagaag ccctgctgtc tctcgccggt cagtttgacg gaatcatttg tggagatgac
    25261 cgttttactc cacgggtgct ggaagcctgt tcaccccggt taaaggtaat ttccaaatgg
    25321 ggtaccggta ctgattcgat tgaccgagcc gcctgcgagc gcctgggtat ccagctaaag
    25381 aacacgccaa acgcgttcac aattcctgta gcagacagcg tgttggggta catcctggct
    25441 ttcgcccgcc gccaaccctg gatggaccgg gcgatgaaaa gcgggaaatg gcagaagata
    25501 cctggtcgcg ctttatcaga aacgaccctg ggggtggttg gtgtgggcaa tattggcaag
    25561 gcggtactca aacgcgccag cgctttcggc atgcgcttgc tgggaaacga catcatcccc
    25621 atccccgctg actttctgca agaatatcat gttgagagtg tatccctgcc tgaactggtc
    25681 tctcaagctg attttatcag tttgaactgc gaccttaatc ccacctcgcg tcacctcatc
    25741 cacaaggata ttatcaggct gatgaagcct gacgccgttc tgatcaacac cgccagggga
    25801 ccggtcgtta acgaaaaaga cctgattgaa gccctgcagc aaaaaagaat tgctggcgcc
    25861 ggactagatg tgtttgaaga agaacccctt cccgcagatt ccgcgttgct acatatggag
    25921 aacgtcctgc tggctccgca taactctaat tccagcccca gagcctggga aaacgtccac
    25981 tggaacagta tccgaaattt actcatcggt cttgccatcg acgcagaaga cctggacgag
    26041 tggaaatcga aagtataaaa ggtgtaccag aaatgaaaga aaacaagcgt gttgtgctga
    26101 tcactggcag cgccggcggc atcggcaaag cctgtgtgga gttgttcgcc gcgaacggct
    26161 ggcaggttgt tggcatagac gtcaatgctt ttggcccggg gtttcccgca gacggtttat
    26221 acattcaggc agatatctct caaccggaag agttccccgt ccttttcgag aaggtcagcg
    26281 ctttctccac caggttgcac gcccttgtaa ataacgccgc tgtccaagta tcaaagccat
    26341 taattgaaac aaccgtcgaa gaatgggaca aggtgattgc cactaatttg cgcccggtct
    26401 tcctttcagc gcgtgaattt cacccgctct taattaacgc gggcagcagc gccattgtca
    26461 atatgtcatc cgtgcacgcc attcaaactt ctgccagtat ctcggcttat gctgccagca
    26521 aaggagggat gttggcgctg acgcgggcga tggccatcga attcgccaaa gacaatatcc
    26581 gcgtcaacgc cgtgctgcct ggggcggtgg atacccccat gctgcgcagt ggtcttgacc
    26641 gcggccatgt gacgggacct tctcttaatg aacgtcttga caaccttgct cgcaagaccg
    26701 tcaatgggcg tattggccaa ccagccgaga ttgcccgggc ggtttatttt ttggccgatg
    26761 gcacacaatc ttcgttcatg acgggtcaag cgctggtggt agatggcggt gccactgccc
    26821 gtttgagcac ggagtgaaaa cccttgcgcg cgaaaatcaa acaactcatc cccgctcccc
    26881 tggtcgacct ataccgctca gcccgtgata acctctcgct ggcattcgcg taccctaacg
    26941 ccaggtttca cccttggcgc gttgattcga tccaaaaact ggaagccttg cgggatagtc
    27001 acagaggtga gcgctgcgtc atcatcggca atggaccgag tttgaagcag actaacctgc
    27061 agaaactacg cggcaccttt tctattggga tgaaccgttt ttatatggct ttcaacgacc
    27121 tgggtttcac cacctccatc ctgctcacgg tcaataacct ggtcatcgaa caatgcgctg
    27181 atgagctgcg cgggctgcca atccccattt tcgtatcatg gcgtgggcgg cgctatatcc
    27241 accccgcgcc taacctgcat tacctgtaca cctcttacat caagccttct ttcaacggcg
    27301 atgccaccgg gcggttgtgg gaaggcgcaa cggtgacctt tgtagccatg cagttggcct
    27361 atttcatggg cttcaagcag gtgattctca ttggcgtaga ccacaatttc accagcacag
    27421 gcaagcccaa tacaaccgtg gtatccacag gcgctgatcc caatcacttt caccctggct
    27481 atttcggtaa agggttccgc tggcagttgc ctgaccttga gacatcagaa atcgcttacc
    27541 gcatggcaaa aagcgcttac gagaaggacg gacgagagat actcgatgca accatcgtcg
    27601 gcaagctcac cgtatttccc aaggtcaact tcgatacgat ctggtaatga aaagcgctcc
    27661 ctccgtcacc attgtcacgc cgtcctataa ccaggcgccc ttccttgagc agacgatcct
    27721 ttcagtgctc gagcaggatt atcctaacct cgaatacttt gttgtcgatg gcgcttcgac
    27781 tgacaccagc cttgaaatca ttcaaaaata cgcccatcgt attacctggt gggtttccga
    27841 gaaagacaat ggccaggcag acgcgattaa taaaggctta cgccgcgcca ctggcgatat
    27901 catcgcctgg ataaattcag atgactatta cctgccaggc gccattcaaa tggcggtaca
    27961 ggcgctgcaa gagaaccctt cttcctcttt cgtttacggg gatgttcagg tagtgaacga
    28021 gaaggacgag ttaatcaata ttctaaggta cggcgactgg ggtttaaaag agctgatgtc
    28081 tttccacatt atcggtcagc cggctgtgtt tatgcgccgg gcagccctgc aaaaaacagg
    28141 ttaccttgat ctcagttatc aattcctgtt ggatcatcaa ctttggctgc gcctggcatt
    28201 gcacggaggt atgactcacg ttccacagtt gtgggcaggg gcgcattacc acgccgggag
    28261 caagaatatg gcgcaagccg ccctctttgg caacgaagcg cagcgcattg tgcaatggat
    28321 ggaagcgacg ccggaattcg ccagtttata tacgcaaaac tcgcgcagga tttgtgccgg
    28381 cgcagaaagg ttgaacgctt tttacctgtt tgacgctcaa gattaccgcg cctctttaga
    28441 cgcttacctg cgtagtttag cgctacatcc agccgctgcc gctcaagatt ggtaccgcat
    28501 cctcttcgcc gccctttctc cgctcggatt ggatcaacta aaaaaagccc acctggcgcg
    28561 ccgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnatcacca ccggaagaag
    28681 cgaacccctg atcgaacctc cggtgatgga atacctatcg gcatatatct atgctgtcat
    28741 cggtaaagaa agtatgctgg taccgcgttt gctgtcaatc gccttctgga tcgtgggggg
    28801 cattccttta ttcctgctga tgcgaaaaat caccactctc aatggtgctt tcatttccct
    28861 ggcgctgtac cttttcaacc ctttcgccat tgtcgccagc cgcagttttc agccggatcc
    28921 tttaatgatc atgctgatcc tgtgggcttt gtacttccag ttcaaatgga tggaagacga
    28981 taagctcgca agcgcgattt ttgccggcat cttcaccggg ttggctgtac tcattaagac
    29041 ccccgcggtt tttttcgtcg gcattccgat gatcggtgtg gtgctgctaa aagggctgaa
    29101 aaaatcatta cgcgattggc gtgtttacct ggtggcagcc atctccctgc tgccggcggt
    29161 gatttatacc gcgctcagcg ccacagttgg cggtaacgcc ggcgccatct tcggcgcgcg
    29221 ctgggtgccg gcgttatttt ctgatccaaa atggtacctg aactggctta tgctcgcccg
    29281 attggtggtg ggcttcttcc ccattgtcat ctcgctgctc gggttctttc tgatccagga
    29341 taaaaaatac caggcgttgt atttttgcat gtggttgggt tatctactct acggcttcat
    29401 gttcgcttac cacatctata cacataatta ctaccacctc ccactcatcc ccgttgtcgc
    29461 gatgggtttt ggcgtgatcg gtggtgaact tttctctcgc cttgaaagca aaaacctgag
    29521 caggttcttc aggctactgg tcttgctgct cttcctcttc gcaattggtt taaatgtgat
    29581 gaaagcacgc ggtgaaatgc ttggcaagag ttacaggcac gaagccaaat attggaaaga
    29641 acttggagaa aagatcgggc aaaacgcaaa taccatagcg ctcacgcatg attacggtta
    29701 ccgcattaat tactggggat ttgttcagcc aaccctctgg cctactcagg gcgatatgac
    29761 cgttaaaaaa ctcattggct ccacagaccc tgaatttgag gctttgttca aaatgcgcac
    29821 tgaaggcaag gattattttc tggttactct gttgggtgat tttgagtctc aggcagatct
    29881 gcaccaatac ctctttgaga actaccccta cgaacagggc gatgggtatt atctctttga
    29941 cttaaaacat ccattgggaa attaggcttg atgatgaaca ggcagccatt ggtttccatc
    30001 gtgacgcctt ctttcaacca gtctccatat ctggaggaga caatccgctc ggttctcggg
    30061 cagacctatg aacgcattga gtatatcctc atcgatggcg gatccacaga cgnnnnnnnn
    30121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngcggcgca aacggacctc aaacgcctgc
    30241 gccgcggtta cgttcacatc ccccagccgg cgaccttctt cagagccgag ttgtggcgtc
    30301 aggtggcgcc cctggatgac cgtttttact tcgcgatgga ctacgatctc tgggtgcggc
    30361 tcgcgcgcct gacagaattc aagtatctac ctggtaaacg atgggcgagt ttccgcctgc
    30421 actctgacgc caagaccatc agagcagacg agcgctgctg gccggaaatg ctgcgcgtgc
    30481 actaccggga tggcggcagt ttcttttcgc ctattgtgct gaaatacttg ttaagaaaac
    30541 tcgccgctcc tttgattaga tggaaacggc ggcgtatgtt taaacgctga caggcgccaa
    30601 gaaagagcct ggtcacctgt catcttgcgt aatgattaaa tgacctgcag ctttttggct
    30661 acctttttaa aggctgccag gccaaagtca aggtcttccc ggttgtgcgc tgctgaaatc
    30721 atcacccgga tgcgcgcttt cccctgggga acggtgggga agcccaacgc catggcaaac
    30781 accttctcag taaagagctc gcggctgaac tgctgcgcca gcggtgcttc gcccagcatc
    30841 accggcgtga ttggtgtgac gctttgcccg gtatccaggc ctatgccttt taattgctcc
    30901 ttgaagtatc gcgcgttttc ccataagcgg tccaccaact cggtggaact ttctaaaaga
    30961 tcaaccgcag cgatacaggc ggcaacatcc gcgggcggag tggctgaaga aaacaagaaa
    31021 ggtctcccgc gttggcgtac ccactcgata atacgcgcgt tgccagccac aatgccgcct
    31081 accacgccga aagcctttga aagcgtgccc acctccacat ccaccttgcc atgcaggcca
    31141 aagtgatcaa cgatgccgcg cccacccctg ccgataacgc cttcgccatg cgcgtcgtca
    31201 accatcagca tgatgtcgtg ccggctggtc acctcataga ttttatccag cggcgccagg
    31261 tcgccatcca tgctgaaaac accatccgtg atcaccaacg cgcggcgata ttttccttcg
    31321 ttttcgttga taaccctctc cagatcggcc acatctgtgt gggcatattt cgcgataggc
    31381 gcgcctgaaa gccggcagcc atcgatgatg ctggcgtgat tcagctcatc tgaaataatc
    31441 aggtcgtctt tgcccaccag cactgggata accgctgtat tggcagcaaa ccctgattgc
    31501 agcaccacga cggcttcaac acctttgaaa cgcgccagtc gttgctcaag ttcggtgtgc
    31561 agctgcatcg tgccggcaat ggagcgcacc gcgccaggtc ctacgccaaa tttcttcaac
    31621 gcttcctggg cggcttttac cattatcggg tggtttgcca aaccaagata attattggag
    31681 caaaaattca gcacttcttt tccatcaacc accagccagg caccctgcgg cgaacccaga
    31741 gttcggatgt gattgtacag cccagccgcc tttagcgtgt caatttcctc ctggacccaa
    31801 tccagtcttt tgctcatttt cacctctttt tgcgtttgct atgcttcacc agtgattaga
    31861 ttatacatct ccggcaattt ccgccagtat tcccagttcg ttaagcagcc tgaggatgcc
    31921 gtcaacctgc ttttcgttaa tgaccacgca gagcgggtcc agcgtttcca gcgcgaacct
    31981 gccggtcgcc tgcttgagaa acgcagccct cgcatcctct cgcgtgaagc gtagcaacag
    32041 caccctctca atgaccgcct cctgcccatg ctgactccag cgctcctcca gcacgcgcag
    32101 attttctggt atggcagccg ctttttctct ctccagcaac tggatcaatt ggccaaggcg
    32161 taatccctgt tcttcagcct tttttaaagt atctgttttt aatgcataca ggctttcttt
    32221 ggggaacaca tgcaccagct cacagaacct ggctacctgg tagcgcggca ggcgcggagc
    32281 gaagtatgga atgctaaaga gcagccggtc ccgaatcttc atcacacggt tttcttcatc
    32341 actgctgctc agttccgcgc cgtcaagcag cggtttcatc ttttcattga gttgaaaagc
    32401 ggtgattttt ccgttttccc cggctctggc caggttaact attcccagcc agtgcagcgg
    32461 ggcgccaagc aaataggtta acagcgcgcc ttccaccttt tcccaattct caaagccgct
    32521 tagataagtg gaagatttcg tctcgcgaat ataccaggac tcataatctc ccccctggcg
    32581 ttggaaatca gggttggttt ctttaatggt cgccagcagc gaagataggc tccaccaggt
    32641 gccgctctcc aggccagcga gaatctcagt caataacctg cgtgggggga ttggatcgtt
    32701 cttccacgtc ccctcgaact ccaaaccagg gagcaggcga agctcattga tttcatttga
    32761 tttaagccat acctggtaga gatgggtgag cacctggccg cgtgtggcat tgaagaattc
    32821 tttcaacgct tcaggaacgg gttgcagtgt atcatcaacc agccctacct cccgcagcca
    32881 tggatagaga aaatcccggg gtatatccca ggcgttccaa atttctgccg ggatttccct
    32941 ccgcatgcgc agcgctgcca aaatatctgt cacgcgatct ggcaggagtg cttcagccct
    33001 ggcaacccat ttcggttttt gattcactga aggacggatg tgcagcttgg gggctggctt
    33061 ccctgtgttt tttgctactt ccagcagttc atcagggata tagaaaaatt ccttgggttc
    33121 cgcttcggtt ctcagaaagg ctcttcccgc caggccggcg taccacagcg tttcagcggc
    33181 actcacaggg tgcaggtctg gttcttcgcg ttcgcggctg gcgcgaccat aatcacggat
    33241 ctgcccataa attctggaga actccgccca gtggattttt ccaccttttt gcaccaggtg
    33301 attccaggct ttggcagcgc catcatccag gccttccagc actttctcaa acaagctggt
    33361 atccagcatg ttctccagaa ggtcctggcg agcgctttct acctcccgct ggctgatctg
    33421 tacatcccaa cgcctggcaa tacgggttaa aaaatccagg tcataattcg ctaaggttga
    33481 tctcagtcca ggcatgcgaa gatctatccc tggtgattat acctaatttg actttttcaa
    33541 cccagttaac aaatctgaat ggttaattga cgctattccc ccttctatgt ataattcctt
    33601 agctccctta aggttcgaac ttaggatggt ggatggcgtt aaaaggtaat ctgaaggatt
    33661 tctcatttat acagttgttg aacctggtca acctggcgaa taaatccggg gctttgtatg
    33721 ttgaaagacc gggagaatct gcccgggtgc ttttccacaa cggcaagctg gagtacttcg
    33781 aatccagtaa tgagaaacct gaattattgc gttccctcaa ggatcaaaaa ctgatcaccg
    33841 cttaccaaca caccctgttg aaagaaaggc ttgaaggttt ctctgaccgt gaggcaggca
    33901 tctacctggt taacgcaggg tacgccactc tggaccaggt aacgacggcg ttggaagaag
    33961 attacgccaa gaagttccgc gctcttttcg attggaatga gggtctcttc aggttcgaag
    34021 ccggagaatt actgcccgaa aacaaaattc ctgtacacct caaccttgag aacctcattg
    34081 tggaaggagc acgggagtta caggaaatcg aagaattaaa ggctgagatt ccgagcctgg
    34141 atatggcaat caaattcact gatcgccccg gaactgacat tcggaatatt tgcttttcgc
    34201 caactgaatg gcaggtgatt tcttacgtca atcctcgcaa taccatccag cagatcgcaa
    34261 ccgccactaa attagacgaa attgaaattc gccgggttgt ttttaccctt cttcaagccg
    34321 gtctggtgga gatcattcgc cctggtggcg tacccctggc aattccggga aagaatcttc
    34381 ccgtcagaaa cccggaagaa aaccgttctt tggtcaaacg cctgatcgat cgtattcgct
    34441 ctatttaaaa acacaggaac ttttacgaga tgcaaactca gaccgtcaaa attgtcgtca
    34501 caggaccctt caacgctgga aaaaccgaat ttatccggtc tgtgagtgag atcgatgtgg
    34561 tttcaacaga aaaacgaatc agcgcgacgg ccgaaaaaat taaagaatct accaccgtag
    34621 cgatggattt cggcaggatc acggtagata acgacctggt gcttttcctt ttcggcactc
    34681 ccggtcagaa gcggttcgat ttcatgtggg aaattctttc agaaggaatg ctgggtttca
    34741 tcgtgatggt agacagcacc agaccagaga catttcgtga agcacgcagt atactggaaa
    34801 cattcaaggc gtacgccccc accccttacc tggtaacggc caataagcaa gacctgtacg
    34861 acgcctggga tactgaggat atgcgtctcg ccttaaggct cgatccttct gtcaagattc
    34921 tcccctgcga ggcaacgaaa aaaagctcgg tgaaacgtgt attactcgaa ctgctttacg
    34981 gcatcctgaa agaaatggat gataatgaag ccagacagaa caaccaggaa gacgaaattt
    35041 cttgaaatga gtcaaacgat gctggaatat aagaacgtgg gattggtaga ggtgctcggt
    35101 gcagaagcga ttgctgattt cgccaaggcg atggaggcac aaccctgtga cccgattgta
    35161 cacggaatac tggtaagagc aggccgggcg ggttttcatt actggttgac gcaagaggga
    35221 gatacgttgg aaaaagccga tgccagtttt cggcttgcgc cgatcaagag aaaaattaat
    35281 cttggcttgc agcatttttg cgccattctt tcaaccgata caaaatatgc gcttgaattc
    35341 aaagactctg caaacaaatg ggagcttgcc ctgacagccg ttgagccagc aggtttgttt
    35401 tctctggaga gcagttacct ttccggcttt ttacaggagt ttgccagctg ggcggggatg
    35461 ggcaggcttt accaggtgca taaaaaagtt ggcgattccc ctcatgctca acacagcatc
    35521 atcattgaaa aagagcctgt tgagtaacga tcggcaagaa cggtttttca ttttgattta
    35581 tcaaaaaggc tcacgaaacc cccatgacag aagatgtatt tatatgcgta gatgtagaaa
    35641 cagccggtcc agttcccggg aagtatgcta tgctctcgat cggggcttgc ctggtctttg
    35701 atacaaccgc gacgttttat atagagctca aaccagataa acccgaagtc gacccggagg
    35761 cgctcgccat cagcgggttg gatatggacg cgcttcagca aaacggcgta gaaccagcgg
    35821 aagccatgcg cgctttcgcg gattgggtca aggtcgtttc tcctgctcct gccaaaccgc
    35881 tctttgtggc ttttaacgcg ccattcgact ggatgttcgt gaacgattac ttccatcgct
    35941 acctaggtcg caaccctttc ggtcacagcg cgctggatat caaagcctat ttcatggggc
    36001 aaatgggggt caactgggca gataccacaa tgcggcgaat ctcctcgttc tttcttgaag
    36061 aacaccgcct ttctcacaac gcgcttagag acgcccagga ccaggctgag atttttcgca
    36121 ggatacttga ggcaaacccc aggttcaatc tagcggaatg atggtttaga aacgccatct
    36181 gttgtgtata ctgttggtgt agttaaccat cttttgtgtc ggatttggag gaaccatggc
    36241 aaaaacgaat gactcccata aggcggacct gaaccgcatc attgaatcgg cgaaaagaat
    36301 gggtgttgag atcaaagaag atgaagccct gcagtggctg acaaacatgg ccgcttataa
    36361 agaaagtggg caaatttccc ttgacacccg cagcggagta tttggtcaca acatcaccat
    36421 gctggatttc agcgacaagg aactggaata cttccgccgc attggtaaga ttgtagagtt
    36481 cgaagatatt ccaggcaagg ttgaaaccgc gctggcgctt tcgggctcat cggctcaatc
    36541 caagattcag acctatcctg gtgattgtga ttactttgaa cgcatcaaca ttatcgcccc
    36601 cacgcgtgat gaagcctgca acatcctggc ggagatcatg cggaaaaagg cgcttgatta
    36661 cctgcaagga cccaattacc agttgatcga ggtcaagttc ggcgagtacc cacaggatat
    36721 tgtcctcggt acccgcgaag taaaagccgg ttctcccatt tcctgggatc ccgcgcagat
    36781 ccgggagggc cgaattacgg gcttcaagaa agacggcacc cccgtaacgg tcgcctggga
    36841 tgatgtgaag aacaacccgg gctggtgcaa gttagattgg gtggtagcag atcccatccg
    36901 caaacagctg gccaacgcca gtaatatgct ggatgttacc tgggaggctc caggtggaga
    36961 cataaccccg ttggatggct accttgaccc ctatttccag gaagtttatc tcgaggcgtc
    37021 ttctgtaccc attttttcaa aactgtcgca gcatgtttca gcgaacgcgc tggaagaata
    37081 tgtgaacgcc ttagagcgcg aggtttataa ataccttacc aaagacgtga actatggcaa
    37141 ggtggccaag cgcatgtaca atattttccg cctcacagga aagtacgaag aagctgcctt
    37201 tttacgcgag ctttttgacg aaccttccac tgttctgtat cagatatggt cattaatccg
    37261 caccatcgac gatgcatata tcccgggctc atcaatcacc aacgaacaaa tggtggaatc
    37321 caccgaccgc ctgatcttgg aagtgattcg cgcgcttgaa ggagacgagg aaaccgagat
    37381 tgccaaacat ctcctcaacc tgcgcacggc cctggcaaac cctgacgccc gtgatatgct
    37441 caccgcagaa gccgaagccg cccgggcaag agtaatcaac atcgtgaata acttcttcta
    37501 cgaaaaactg gttgcctatc caacgattaa agagtacatg gacgaagtgc aaaccagaag
    37561 tcattgaaac ggctttgcct atctcatggt cacagagggt tttcacctga aggatggaaa
    37621 ccctctttct cgcggcaggt ggcatccagt aattgcggag atacttttct caaacccggg
    37681 taacgcatgg atggtagaat aagcgtatga cgatcgttac gcagtccggc tcccaattct
    37741 tcacaaagag gtttgcttga acaatagtca tcagagtatt tcagaaggtg ttgcagtaga
    37801 actgtacctg gacgcggcgg cagaggagca ggtgctgaaa ttccgtgagc tcatttaccg
    37861 tgaaggggta caaccagtac aggggctgat ggatgacaaa cctcatatca gtctggcggt
    37921 atttcccatc acagaccctg ccgcggtgat cgatctgacc agggaatttg ccctgacagt
    37981 cccccgtttc agcttcaggc tcggtgcggt cggtacattc cctactcaag acaacgtcct
    38041 cttcctctcc cccgtaccca gcgtaccttt gctgcaggtg cacgcagcct tccttacccg
    38101 ggtcaaagat gccggtatca attgttcgcc gtattacttc ccaggtgttt gggtgcctca
    38161 cctgacgctt gagtttgaaa tatcgaatga tgagctgtgc cgttcgatga aagtatttaa
    38221 ggaatcgttt acccctattg atggagaatt cacccaactg ggtgttgttc aattcagacc
    38281 cattcaatac cttgaccagt ttgatctaca gaaaggaaat taatgtcaga aggtgtacca
    38341 cccccatcag aagagcaaat tcgcgatgcc gtcaaccgca ggaaaaccaa agccgaggaa
    38401 tacttgcagg acccagataa aacacgccgc ctgcttgaag aggctgtgca taaagccaat
    38461 gatcatgaag gaaaaccggg gcagaaacta gatttctggc tgcagttaaa agcatttttc
    38521 aggttgttgc gagcctatac aaatcgtgaa tacacggtcg ttccttgggg atcgattgtg
    38581 atggtcacaa tcgccattct ctattttgtc tcgccgattg atcttattcc agattggctg
    38641 ccattggccg gctttgtgga tgacgctgct gtcattttat tcgtcatccg ccagctcaaa
    38701 caggatattg acggttttct cgcctgggag aacgcgcaaa aaaaccccgg cggacaaatc
    38761 attgatcttt aatttcaagc acgctccatt ttgatcggaa aattcgatat aatggaatgc
    38821 aaactaatgt ataggaggag taaatgagta aaaagtgggt aacggttcta ttagtggtat
    38881 ttgtgttgat gtttgctctt tcagcatgcg gctccctcaa agatgtttca aaattgggag
    38941 ataagttcat gtcagccctt aagacccagg acaacgagac ctcttacgcc atgctttcac
    39001 ccgatatcca gtatgaagtg ggttatgagg ctggctgggc tgaatggacc tccatccgcg
    39061 cttttaatga ttggaagttc accagtaagt ccgttgagaa taacctggca acgctggaag
    39121 gcaccgcgca cctggatggt gaagagtacc tggtgacctt ggctttcacc cgtaatggtg
    39181 acgcttggga gataacgaca atcatatttg aacttccgta gcccaaaaag tggagactgc
    39241 ccgtctccac ttttttttac aggaacgttc tcatcactct ctgtcacact ttataaaaca
    39301 ggatggattg cgggtctcca tcctgtttcg tcatcaccag ccaaaaagag aaacggccca
    39361 gtcattctgc agagctgtat gtgaccactc gtcaacttgc gccgccctac ccagcatgat
    39421 acacttttac ctatgaagat caacctgctc gccttctctg ctgatcggca gctgctgacc
    39481 agcctggaag tcatcacttt caatgggatc acgcttacca ccaccccggc gggtacaagc
    39541 agcgaattgc gcgcccttac ccagcagcag cgctttgacg ccgccttgat cgacgcggcc
    39601 gacggtcatc tgcctgtggt gcaaacaatt ctcaacctca ccgctcagca gccggggatt
    39661 aaaattttgc tgctggttgc tgatgagcag aacgaaaccc tgccgcctga agatatcgct
    39721 tatgatagat tgctgtctcg acccctcagc cctgagcaac tcatcagttc actgaaagaa
    39781 ttgttcctgc aacctgagga aacctgcgcg gaaacaggcg cagcgataag ggaaacccca
    39841 gcagcacaag cagagcagga acccagcctg attgagatca tcgacgctgc agaaaatgaa
    39901 aaccgattga cgggcgaaca aggcggtgcg ctgaccagcc acacggtgaa gagcgccggt
    39961 aatgcggatc ccaatttgat tgttaaagag ttgcgcctga gctactgctg cgtgctgctg
    40021 ccgtggagtc ctcatcaata cctgacgggc gaactctccg atcacgccgc cgacttcctt
    40081 ccccgcctgc accacagcct gggctggcgt atgaccggca tctccatccg cccgcaatat
    40141 ctgcaatggg tcatttcgtt gccggttgaa acctgccccg tcacggccat ccagaagatc
    40201 cgccagcgaa cttctgcaaa ttttttcaaa acgtttcccg acctgccctc cagtggggag
    40261 aacagggact tctgggctcc aggctacctg atgttgagcg gcgtgcaaac cgtaccttcc
    40321 gccattattc acgaattcgt taaacgcgcg cggccagctg acggctgaac agcagaaaag
    40381 gatcatttat gcctccaatc ctctatttaa tcgatggtca cgccctggcc taccgggcgt
    40441 attacgctat caccgccgga atgagcgacc gtatgcaaac caaaacaggc gaacccaccg
    40501 ccggtatttt tggttttgcc aatgtgttga tgcgcctgct ggagcaggaa aaacccgagt
    40561 acctggcagt tgcctttgat accggcaaaa ctttccgcaa cgagctcttc cctgattaca
    40621 aagccacgcg ggcaaaaatg cctgatgacc tgagacctca aattgaacgc atccgccaga
    40681 tgattgatgc ctttcacctg ccaaggcttg aaaaagaagg ggtggaagca gacgatgtac
    40741 tgggctccat cgccactcaa gccgttgagc agggtctggg tgtcaaaatc atcaccggtg
    40801 accgcgacct gctgcagctg gtgaacgacc gcatcatcgt caacctgccg gggtcgcggc
    40861 ttgccgatgc aaaaaattat tctgccgctg atgtgaagga gtacctggga gttgagcctg
    40921 aacaggtggt ggactataaa gcgctggtgg gcgacacctc tgataacatc cccggtgtgc
    40981 ccggtattgg ccccaaaacg gccgttaccc tgctgcagac ctaccacacg ctcgatgccc
    41041 tttatcagca tctggatgaa attcccggca aaacggggga aaaattacgc gtcggtaaag
    41101 aatccgccta tttgagccag gagctcgctc gcattaaaaa agatgtgggg gtaaggctgg
    41161 tgcttgaaga cgcccgtact gaccacctgg acatcgcatc ggtggaaaac ctctttcgtg
    41221 aactggagtt ccgtacgctc atcccgcgcc tgcgattgat cgcggctccg ctgaaaaaac
    41281 aagaaacccc gcagctttca ctttttggcg aagagatgaa ggagattatt gtgccagagt
    41341 cctcttatat gattgaggtg aaactgatcg acaccgcgca aaaactggcc tcgctcaaac
    41401 aagcactgga tgccagctcc ctcatcgcct ttgataccga aaccaccgcg ctggatcccc
    41461 tgcaggcgga gcttgtgggc ctttctctcg cggtaaaaga gggcgaaggt tattacattc
    41521 cgctcggtca taagggcgaa caacctcaac ttaaggtcag tgaagtgatc gaagcgctca
    41581 caccggcgct cacaaacccc agcatcgaaa aagccggtca caatatcaag tacgacgcgc
    41641 tcgtcctcgc caggtacggc ttgcgggtat ctcctctctc ttttgatacg atgatcgcgg
    41701 aatggctcac caatcctgcg agccgcgacc ttgggctgaa agatttggca ggtacattgc
    41761 tgggtatccg catgacgcgc atcgaagaat tgattggcag gggtaaaaac cagctcacaa
    41821 tggatgaggt gcccattgca gcggcagccc cctacgcagc cgcagacgcc gaggtcacct
    41881 tgcgtttaat ccctttgctc aaagaaaaaa tgcaaaaaac gaacagcacg ggcatcttta
    41941 ataccattga gatgcccctg gtaccggttt tgctcaaaat ggaacaggaa gggatctcgc
    42001 tggacattga tttcttcaaa cgctttagcg cagaaatcgg cgaacgcctg cgcctgatcg
    42061 aagacgaagt tcatcaggca gtcggtcacg ctttcaacct caattcttcc cagcagcttt
    42121 caaaggtgct cttcgaggat ctcaaacttg agcctcctga ccgaaataaa agaacctcca
    42181 gcgggcattt ctccacctcg gcggcggtgc tggatgaact caaagggcag cacgtggtgg
    42241 tggatctgct gctggaatac cgagaactct ccaaactcaa atccacttac ctggatgccc
    42301 tgccccagca aataaaccct cgtgacaatc gtgtgcacac cagcttcaac cagaccggtt
    42361 cggtcaccgg gcgcctggcg tcatctgacc ccaacctgca gaatattccc acgcgtactg
    42421 aaacaggcag gcaggtgcgg ttgggcttca tcgccgcgcc cgggcacagg ttactctcag
    42481 tggactactc gcaaattgaa ttgcggatcg tggcgcacat gtcaggcgat gaagccatgc
    42541 tcaaggcgtt ccgtgacggg caggatattc acgctaccac tgccgctgcc atctatgacc
    42601 tgccgcttga gcaggtgacc aaagaacaac gccggcatgc caaggccatc aactttgggt
    42661 taatttatgg gatgagcgcc tttgggctca gccgctccac tgacctgacc cttggagaag
    42721 cagaaaactt cgtcagggat tattttgagc acttccccgg ggtcaagaaa taccttgatc
    42781 agattcgcgt gctggccgcg cggcagggct atgtggagac catgctgggc aggcggcgtt
    42841 actttcctaa cctggccaac ccgctgaacc agatgatgcg caaccgggaa gagcgtgaag
    42901 ccatcaacgc gcccatccag ggaaccgcgg ctgatatcat gaaaatcgcg atgatccgcg
    42961 tacagcgggc gctggaagaa agcgcgtccc gcgccagaat gatcctgcag gtgcatgatg
    43021 aactggtact ggaggttccg gagaacgaaa ttgcgctcac cagccaactt gtccgcggga
    43081 ttatggaagc agcttacaaa cttgatatcc ctcttcttac agaagccaga agtggtgtaa
    43141 actggggaag tatgcaggtg attgcatctg aatagcaggc tcgggagcag ccgatgaacg
    43201 tgacagatca ggctttgcag gcaaaactga ataacggaca cgcggcttta tgggagcacg
    43261 attgggataa cgcggttaga gtctacactg aagtactgct cagtgcccct gaaaacccaa
    43321 tcgccaaagc cagtctcggt ctggcgaagt ttcaccagaa gaagtatatt gaggcgttgc
    43381 agttatttca acagcttgcc gcacaatatc ctgaagaccc catgccaatg gaacgcattg
    43441 cccgtattta cgagcgcgag gggctgctgg ctgaagcgtc taccagtttt ttccgcgctg
    43501 ctgaactaca cttaaaagcc aaagatgttg aaaagtctat cgctgatttt caggccgtca
    43561 ttcgcatcga gccaaataac cagatcgcgc gcgcccgcct ggcgttgatt tatgagagac
    43621 tgggcagaaa gaacgaggcc gcggctgagt tccttgacct ggcggcagtg atacagcagc
    43681 atggcaatat cgacaaagcc cggctgatgc ttgaatacgc gttaaagtta aaacccgacg
    43741 ccgttgaagt tcacaacgcg atggcaagtc ttaagaagaa cgagcctttg cgcctgcgcg
    43801 atcgaagcgg agaaccgact ggcgctgcgc gtatggcaca ggtgagggag atagaaacag
    43861 cagttcagga acccgaagag ctgaccggca atgacccgat gactgaagcg cgggtgatgg
    43921 cgttgaaaga aatggctggt tttctgtttg aagaccagga aaccaaccgt ccggccgtca
    43981 aaccacggga tcttcaaaac ttcgtgccag ccagcagcgc gagcgaaaag gcagaagcag
    44041 ccagccacaa gcgggttctc taccacatca gtgaatgcat cgacttacaa acaaaaggag
    44101 aaaaccttga agccgcggct gaactggagc gggcgatcaa catcaatctg gacctctctg
    44161 ccgcgcatta cctgctggga tatttgctcc gtgaatctga ccctggtaaa tcatatccgc
    44221 acctgcaaaa atcagtgatg gacccggcct acgcgctcgc ttcaaacctg atcatgggag
    44281 agatccattt tgaagccgcg cagttaaaag aagcttcctc caacttcctg catgccctga
    44341 tgctggcaga tgctgaaacc gtgccacccg aggaggctgg cgacctgttg cagctctacg
    44401 acccaatctt tgaatcgctg gcttttatca cgcatgagag ggacctgagg aacctttgcg
    44461 cggtcattcg tgaacaattg agccgacctg attggcgcga ttacctcaaa gccgcccgcg
    44521 agcagctccc gcccgcccct gagggcagcc cgccgctgcc gctggctgaa atgttgatgg
    44581 actccaccag cagtcaggtg gtggaatccc tggctgaagt gcgccaactg gcgatgcagg
    44641 ggaagaaccg ttccgcgatg gaagaagcct tcagagccct ttcttacgcg ccctcttacc
    44701 tgcccctcca cattcaaatc ggcgaaatac tggtaaacga agggcgtatt caggaggcgg
    44761 ttgaaaagtt cattcaggtg gcgcgtctct acaccgtccg tggagacgct gcgcaggcta
    44821 tccgcctgct tacccgggtg accagactgg tacccatgga catttcagtt cggcgcaacc
    44881 tgatcgacct gctgcgctcc aatgggcgca acgctgaatt gatcgagcag tatatggacc
    44941 tggcgaatgt gcaatatctt ctggctgacc ttgaacaatc gcgcaacgcc taccattccg
    45001 ccctcatgct tgcccgccag tcgcggtcta cccgggagca atcgataaag atcttgaacc
    45061 gcctggcgga tatcgaaatg cagagcctga attataaaga agcggtgaaa gcctttgaac
    45121 agctgcgcag cctctcgccg ctggatagat ccacacgcgt cgccctcgtt gacctgtatc
    45181 aacgcctgga gatggaatcc gcggcgctca acgaagtgga cgcctacata aagttgctgg
    45241 aagacgagaa tcgcctgggc gatgccgaaa acttcctgga tgctatactg agtgaaaaac
    45301 cagattccga tggtcttcaa cgttgtatgg tgtcctatta cacctcacat aaaaaaacca
    45361 gcactgcgat cgaaaaactg gataagctgg cggagaaatt actggttgag aataacgtaa
    45421 aagggtcgct tgcggttgtt gagaagatca tctcgctcaa cccgcccaat caaacagaat
    45481 atgaaaggct gcgcgcggaa cttctcaagc gctaaacgaa agaccagagg attggtcacc
    45541 cttcatcaga tggatgaagg gtttttaata tcctttttac caccagattc tgaaccagga
    45601 gcgcccgctg ccgtgctgcc ctctgaaagt gatatcagcg agcaccttga cgaccgattt
    45661 tacggggatt tgcggggtgg tgaacgtaaa ctggctgatg ccgtgctcat tcgtttcggg
    45721 caggcggtag aactcgcgtg tgtcaggcat aaaactcagc gtcacgccca cctgcgcacc
    45781 ctcaatcggc cgcgcgtatt gatcttgaac gaccacaaat acgctctgtg attcctctac
    45841 cccgatcaat gatttttggg taaacacccg cacccgcggg gtcatcaagc tgccactcag
    45901 gttgggagga gggttggatt taagcaactc agggtcagcc accactcgat caaaataaag
    45961 cttgcccagg tcagtcaaaa ccacacgttc gcccgcggca cggtctggcc accattccat
    46021 tcgcgcgcgc gtaaaatact gcacgtaacg cccatcctcc acctccacgt ttgaaagcgg
    46081 catgccaaag tgggtttcgc cagcatgggc ttcgtaatat tgtaaaaacg cgtaacatac
    46141 cgtaaaacct gtctgatacg ccttacaggc aggtccttcg cgggggatat tggccagcgg
    46201 gttgccagct tcgtgcagca gttcacccaa aggcgcgacc ctcaccacag gccctgaagg
    46261 ggtttccacc aggtcaaagc gcgccttttc aaaatactgc acgcgtgatt tgctaatggg
    46321 gtcggtgaaa tcatcagtga tagggtaacc aaaatacagc agcgggtctg acacactatt
    46381 gtaagtgcgc agaaattctc cccagatcca gtgacctgtt tcaggaacat aaaccgaatc
    46441 tgattcctgc gcccgtaccg cccctgaaga cgacgccccc agcagcgcag acacgatcac
    46501 gatcaccagc aagcggataa gttttcgcat ttattgcatt atacaaaaac cgatgactat
    46561 tcgcaatcag aacgggtttt tccgcgcgcc aaccgctgcc cccgcgcttg cacatcttct
    46621 gcggagggat gcataaaacc atgcgggtta gtttttttca gcctggcgat gaacgaaaac
    46681 gccagaaagc cggcaaaacc caccagcccg gcggcatacc acatcgcccg cgagtagaac
    46741 acgtcgctga cataccctgc cagcagcagc cccagaccgg taccgatacc ccaggtgagc
    46801 gcgtacaggc tcatataacg cgcgcgcata tcttccggcg ccaggctggc ggtgaaggtg
    46861 gtggtggtgg gcacaaggat catctcccca atagtggcag ccaccatgca caaccagaac
    46921 caccaaaaac cactgccaaa ggcaacacaa aagatggcaa tggcgtaaat cagagcgcct
    46981 gtcgtcatgg ctgtttcagc acggaaacga ttgacctgcc gcgttaccac caactgaaaa
    47041 agcacgacca tcaaggcgtt ggtcatcgga atgaacccga acattttttc agagaccccg
    47101 aaattttgct tggcgtacac cgccaacagc agccacagga tcgaggtact gatacgattc
    47161 aaggtgtatg cccccatcat taccataaac accctgtctt taaagatcct caggtacccc
    47221 ccgctggttg gtgctgtcac caccacgggt ttgcctgacg ctggctttgt ctcaatgatg
    47281 agcagcgtga caaaaaccgc gatcagccca aaccccaccc caatcacgcc gaacgttatc
    47341 ccataggaga tggacgccag gtagccgccg atcgcgggcc caaggacaac gccgaaatta
    47401 ttgcccatgc gcagcacgga ataagcgtcc agccggttgg cttcagggat gagatcggcg
    47461 accatggcgt cagaagccag gcggtacaaa ggggtgctgg cgcccgtgac caccagcagc
    47521 agcgcgaaaa ccggcagtgt gccagccgca cggaagagaa accagcccag cgcggaaaaa
    47581 atcagcgaga agaccatcac ccctttacgc ccgaacctgt ctgcaatggc gccgcccatg
    47641 aaggtggtca ccatgccgac cgcggcgttg agagtcatca accaggccac gctggacagc
    47701 ggctcaccca gtcgttggct gatgtacagc atcaagtacg gccatatcag gctgtgaaac
    47761 agccacgcta gcagcattat caaagacacc agccaaaact ggcgcgggta tttcttcaag
    47821 atagtgctaa acctggaaat cacgcgcata attctaacac gcacttctcc aatccagggg
    47881 agccgtccat tgacagagcc cttcttttcc agtaaattga atccgtttaa acgatctcgt
    47941 tcaaggagca aaaaatgctg gaagatttta agaaatttat aatgcgcggc aacgtgatgg
    48001 acctggcaat tggcgtgatc attggcgggg catttagcaa gattatctct tcgctggtag
    48061 acgacatcct catgccgccg attggtttac tgctgggtaa ggtggatttt tccaacctgt
    48121 tcgccagttt aaccagcgcc aggtacgcca ccctggcgga ggcgcaggcc gccggcgcac
    48181 cgacactgaa ctatggtgtc ttcattaaca atgttgttga tttcttgctg atctccctgg
    48241 ctattttctt gctgatacgc gcaatcaacc ggttgacgaa aaaacaggcg gaagccgccc
    48301 ccgtgacacg cgcctgtccg cgctgcctca gccagatccc ccttaaagcc agtcgctgcc
    48361 ccgcctgtac ctccgaagtg acgccgcaat aaggcaggcg ctgacgaagg ttatgtaaac
    48421 tccgcgcgcc ggtcattcac ctgctgccca gaaagggcgt ctcactgcag cgcgctgctt
    48481 cactttctgg caggtttgcg cccggttgca atcaaagcat tctgtcagga gcgtgctttg
    48541 cgggcggggg ttttaatcac cccgacacga taggaaggcg cgagtatttc aatgatcgag
    48601 cacagccgcg tatccagcat gcggctgcgg atgcgcgcgt ctaaatcctc cagcgtggtg
    48661 gacatggtga tgaccgtggg atatttggcg ttgtaccttt cattaaatat ctggtacagt
    48721 ttttctcgcg cccagggggt ggcgctctgc gtgcccaggt cgtccaatat taaaatcggc
    48781 gcagaacgca cccggctgaa caggtcatca tacgaggtgc cgcttgttgg attaaaagta
    48841 gcccgcaggt ggtcaagcag gtcgggcacc accacaaaca caggggcgtc gcccatgcct
    48901 ttgcggtaat tgccaatcgc cgccgccagg tgtgttttgc cgcagccgta ggtgccggtg
    48961 agcaccagcc aaccgcgcgg attttctgcg aactcctgcg ccgccctgaa tactttctcc
    49021 aggttcaact gcacttcagg ggcaagttgc tcgggctcgc gcaggctgaa gttgccgaac
    49081 gagcggtcgg aaagcaattg cagttgatag agcagcgggt ctcgtctttc cttgacgggc
    49141 gagcggtaat ctggcgcgtt gatatgcacg gtcgtcacca ggtcagggtc ctgcaagcgc
    49201 gagcggatgc ggtcatccag gtcttccagg tcaaggttgg tggtgatgac cgtgggcagc
    49261 ttgcgcgtgt agcggtaatc gataatttga aataatttct ctgccgccca ctgggtggcg
    49321 ttgtgcgcgc ccaggtcatc cagcaccaat aaaggcacgt tgcggatctc ctcaaagcgc
    49381 tgttcaaagg tggcgtcggt ggcatcatac gagtaccgca gccagtccag cagatccggt
    49441 acggtgagga agatggtggt catgccaaat tccacgcagg tgttggccac ggctgccgcc
    49501 aggtgcgttt taccgcagcc gtaagacccc atcaacagca gccagccgcg cggtgcggcg
    49561 gcgaactgct gcgcctggct gagcgcgtat tgcagcgagc ggatttgctc atcagccaag
    49621 cccagcctgc cctgaacgga ataagaggag aaagacatgt tcttgaaagc cgccaggttg
    49681 ctcaactcgt acaacctgtt gcgggcagcc tcgatcattt tattctgctg gcacacgcat
    49741 ggcaccagct tgccgaattc cgggtgttcc agcggcaggt cgcgatgtaa gaagcccacg
    49801 ccaccgcaaa ttgggcaaac ctctgagggg gcggagggtt catcaggtga tgatgaagtc
    49861 ggcatacttc ccttcgctat cccgtttgcg cttttcttca gcggattttc gatcggttcc
    49921 atcgcgccct ttctctttcc atgatcttaa tatcgcttca atatactgcc agttacgcac
    49981 attcctggtg accgcaatgc ggatcgcgtc ttcgatccac tccgcggaat aagtggcttc
    50041 ggcatcttgc agcgtttcgg agagtatcgg cgtcagcggt ccgatattct gctcgtacag
    50101 gctgaatata tttgggcgtt ccctttgcag ccgcaatggc agtgactgcg ctgtttcagg
    50161 gtcccaggtg ccgtttttca gccctaccag ggcggctttt ccttgctcgc cgttgagaaa
    50221 gaaaaagcgt ttatctttaa agcgtgcttc cagcagtgtg ccgcggtcgc aggcgcgccg
    50281 cagcgcttct tccagtcggg ttgtttgttc atccgttgtc gtaccgaaag aagccatcaa
    50341 aaggttatct tgcaaaatat ccatcaaacc caggtagcgt ggttccttta tctgtccgga
    50401 tagataccag aaggcgtaga gagtaatgcg catctcgtcc aggttatcga tcaccgtcag
    50461 caggtgggtg aaaaaggtgg cgggcaacgg gatcgattcg ttcctgccag cagtgaatcc
    50521 gtcaaattca ggcatgatgg tttttttacc ttttttccgg cgctggttca ctctcagcag
    50581 gggcggcgtt tacaaatttc gccagctttt ccaggaaaac cactcctatc ccggggctgg
    50641 ttggaccgtt cctgtgcttt gcaacgataa gattcgtggt tttgtcgctt tcttttgatt
    50701 tgtgaatgaa aataacaata tccgaatcct gttccaggct gcccgactcg cgcaggtcag
    50761 agagcacagg cttcttctct tcgcggcttt caaccgcgcg cgaaagctgc gcagccgcca
    50821 ataccggcac ctgcaattcg cgcgccagcg ccttgaggct gcgcgaaata tacgagacct
    50881 cctgcacccg gttttcgtta cgcgagtcgc tgctcatcaa ctgcaggtaa tccaccaaga
    50941 ccaggtccag tttgtgttcg ttatccagtc ggcggcattt ggtgcgcatg gccagcgggg
    51001 taatggcggg tgtatcatcc agccagatgt tggtagccga cagcacatcg atcgccttgg
    51061 tgaacacatc ccactcatgg tcttgcagtt tgccggtgcg caggcgcagg gtatcaatgc
    51121 cggtttcctg ggcgatcaag cgctgtacca gctgctggtt ggacatttcc agcgaaaaca
    51181 tggccacgtt ctttttatac ttctgggcag ccgttttggc gaccgagagc agaaagcctg
    51241 ttttaccagt gcccgggcga cccgcgacga tcagcaaatc tgatttctgc atccctccca
    51301 acaacttgtc caggtcatac agaccggtcg gcacgccgga tagctcttca tcccttttgg
    51361 attgctcgtc tacatagtca tagtactcgc tcaccacctc attcacgggt ttgaggtcat
    51421 ggcgcacgcg tgacgcgcca aggctgaaca cagacttctc gacctcatcc aggatggtgc
    51481 ggatgtcctt ctcctggtcg tacgccaacc tggccatttc attggcggcg gtcagcatgc
    51541 ggcgccgaat ggcggcttgc tcaacaaact gcgcgtacgc cgcgccatgc agcgacgagg
    51601 gcgtctggtt caccaagccc agcaggtaag cctgtcctcc aatttcaccc aactgaccct
    51661 gttcctgcag gtcttcgctc acagtcagga tgtcgatggg aacgcgcctt tcgtgaagcc
    51721 ggatatacga cctccaaatc cactggttgc ggatgatata aaagtcgtca ggcttgatga
    51781 cttcggagat ctcataataa gattccgggt tgatcaacac ggatcccagc acagcctctt
    51841 cggcttcacg gctgtacggc tgtgactgga taacggtggc ggcatcatcg aaatcgctca
    51901 tggttttcct tcttcaactt ttttaagagc gtggcttgtc atcttggttc ccctggttgc
    51961 cctcacccgc cgcgctgtca tcctcctgcg taccgctgtc accagccaca tcgtcaaggt
    52021 tcacattttg cagaaattct tcaaacaaag aaaggcgttt gctcgtttct tcgctttcca
    52081 tatcgctttc tgcttctggt gtgggttcgt cctcaacatc cagcacttca gcctgaatat
    52141 ccacctcggg ggtgagacca gcttcacgca agatctcgct gttcaccagg atgggtacgt
    52201 gcgccctcac cgccagcgcg atcgagtcag aaggtcgggc atccacatcg tacagttttc
    52261 catccagttc aagcaccagg ttgccgtaaa acacatcctt cttcagggaa acaacttcca
    52321 ccctgatcaa gcgcgcgcca agtttggtga gcgactgcag caacaggtcg tgggtttggg
    52381 ggcgggcgac ttcgatctcc tgtaatgaga tagtgatggc ctcggcttcg taaggaccga
    52441 tccatatcgg cagataacgc tcgcggcccg attcgcgcag catcactatg cgttgctggt
    52501 tggtcaggct caccctcacg ctatcgatga ctacctccac catacgggtc acaaacaacc
    52561 tccaaaggta ctgctggttt cgctgattaa tattctaaca cgtcggtaaa gcgcgcgcaa
    52621 cttgaaggga gatgaaggac ggtttcttaa ggaaagcctt tcaaagaact tgctttttta
    52681 aggatggggt ataatactct tactttgcca ggcgggtgca gaaatgagtt cagcgcttga
    52741 gggtaacttg tagaaaggca gcccgccgat ctttcgcacc cgacaagtaa atatcacctg
    52801 ctgtattgcc attcatcatt tgtcgactat tgaaacacaa ggctttatcg aattgtcgaa
    52861 accacgcatt ctactgatcg aaggtaaacg caccgaccac ccttcctttg ccgccgggtt
    52921 gaccaaaaaa ggctatctgg tggaaagcgt tcccagcggc gcggctgcgc tggcgcagct
    52981 ggacctggcg gtgccgaacc tgatcgtggt ggacgcttca tccatgcgca ccaacggaaa
    53041 acgaatcgtt caggcgctgc gccagaaaca gcccgggctg gcgctcatcc tggtgattga
    53101 cagcaacagc gaccccaacg accactacga cgccgatgtc atcctggtgc tgccctttac
    53161 catgcaaaag ctcatcaacc gcatccgcca cctgctgccc gggcaaaaca actatgacct
    53221 ggtggctggt cccatttcgc tggatgtgac agagaagcgc gtcaccattg gcgacaggca
    53281 ggcgcggctt acgccgcggc tgaccattct gcttaaaatt ctcatgcagc acgccggcga
    53341 agtggtggaa cgcaaagcgc tcttcagcca ggcgtgggag accgactaca ccgtcgacac
    53401 gcgctctctg gatgtgcaca tgagctggct gcgccaggcc atcgaggaga acccgcgccg
    53461 tcccaaatta atccttacct tacgcggagt cgggtacagg ctggcggtgg agccgataac
    53521 gaaacctcgc caacgcaagt aatcttccaa aaaatcccag ctttttaagt aagcgttctt
    53581 gctccaaata ccgggtaata cgtaagaact taaaatcagt aactaccgat aatcacgata
    53641 gttattgtgg tattggtatc ttatttcatc aaatcgattg acaatcctgg ttgtgaatag
    53701 aagttcgagc cagaaacatc aaaaatgacc ttaccactcc cggtggtaat tatccggtag
    53761 ccaaacaatt gggtttcata ggttgtggtt gtaccagacc ctgccacatc aattgttcca
    53821 tatggcacat aaatcgttcc tgagaaagtt gaaccgctga tccctactaa attcatttct
    53881 tgcgtagatt tctgattggg gttactgggg ggttcgtcca tataaatcaa cagcccgcat
    53941 acacctggcg gaacaccagt cggcgtacta ccgccactac aatttggcgc tgacatgagt
    54001 aattggacgt tggacccaac attaaaattt ccatctttaa tatagagtgt aactccctgt
    54061 gcggtcactc gcgcgccagc attcgcatca aaagcgttca aacttgtgct gttaatacag
    54121 taaagaccag gcgaaaaaat taatggggtt ttgcctggcg cttggatgaa tgtatagttg
    54181 ccgggagaaa ttgttccccc tttggtgcta cttccacgat taagcagtcc tgaacaatct
    54241 ggtggtggaa tattaatcga tacaggttgg gtcgctcttt gaggagctgg ggttgaattg
    54301 ctggaataat cagtatcaat atatgaaatc gtccccccgc catacaccgt aactggtcca
    54361 tttccataca aacatgagtt tgaaaaaacg ctgcccccaa tcacattaat gggttttgac
    54421 gcagggtcga accctccttg aacaccacaa tttggtgata gtgatacaat ccccatacca
    54481 ccacccgtag atccacttaa agagactctt gatgtcgcct ccactgtggt tcttaatggc
    54541 tggttggtaa taacctgtcc aaatcgttgt tctgatattg ttgacacaat cgctttgatc
    54601 ttgagataag tcttaccagc ttcagttcca cattcaacat ccacgccatt atttgtagaa
    54661 agatcaaagg gtaataaatc cacaccatcg tcttgggctg aaaggcgagc cgcgttcacc
    54721 gcggctgtcg cggcttgtga agtcaacgtt gagccacaag tgaactgact taagtttccg
    54781 gctttcaaga cctgagaggc tgccccagct ccagctaata cagctgaatc agcagtactt
    54841 tgatctgccc gccgggaatt gtagaccagg ctgccgtcaa ttgcaagcgc tgtaataccc
    54901 aagatggcta tcattgccaa cgcaataatc accaaaacct gtcctttttc tgttttccta
    54961 ttcattattc acctctttca atttctgttt tcccctctaa gggcgaagta caattccatt
    55021 agctgtcgcg cgtaatgaaa cggtttgccc ggtaagcgct cctagaaagg gcatggtaat
    55081 gggaaatggc tgcgtcactg taatctcaat tttatctcca gccttagggg gtgtccctac
    55141 tccacacgcg cgattattga ttagtattga aactgcaact gatccatcct tgggcaggtc
    55201 cgaagaacta ttccgtacac gattattaat attattgcac tcggtgggga aacctgatgc
    55261 ataaataatc ccctcctctg ctgcatcttg cagcgcgaat tgcacaaata tcatgcgccc
    55321 aatatcaaaa attccagaca gcagtatcaa caagattaat atgccaatcg ctaactcaac
    55381 caaactctga ccttgctccg aattgataac gcgattttta taacgaaata ttctcacgac
    55441 cctcccacct tttttattaa aactagtttt tcaatcacat ctttcctaag aagaaatcca
    55501 aatcatgtat aacgttttag acatccagac cgatcaccaa actgcgtgcg ctggttgatt
    55561 cgatttgaat tgtattaaaa tttaaaagag gaccaacaaa aggtacaaag gtagccctga
    55621 cgg
//
LOCUS       DAOTLW010000006        54519 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_700_length_54519_cov_10.998770, whole genome shotgun sequence.
ACCESSION   DAOTLW010000006 DAOTLW010000000
VERSION     DAOTLW010000006.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 54519)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 54519)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..54519
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_700_length_54519_cov_10.998770"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..597
                     /locus_tag="P5516_01815"
     CDS             <1..597
                     /locus_tag="P5516_01815"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF013568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HRT91206.1"
                     /translation="DMQDTYFLDVPNRGDNPLVLRTHTSPGQIRAMREIASLNPSNPP
                     PVRVIVPGMVFRYEQVDVRHETQFDQLEGLAIGEGITFSDLKGTLTDMARRLFGPSAR
                     ARFRASYFPFTEPSAEFDIQCFQCHGKGCGVCGGGWLEIGGSGMVHPVVLANGGYDPA
                     RYTGFAFGFGVQRIAMLRYGINDIRYFWENDLRFLEQF"
     gene            684..3224
                     /gene="pheT"
                     /locus_tag="P5516_01820"
     CDS             684..3224
                     /gene="pheT"
                     /locus_tag="P5516_01820"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HRT91207.1"
                     /translation="MKAPLSWLKDFVEIDLSLVELAHRLTMAGLEVESIQLIGLPLPP
                     AGSTTAKVSGLTWEPEKIVAAAVTEVMPHPNADRLVLCRLEDGQQEHIVLTGAPNLFE
                     YKGKGPLPQPLKVAYAKEGAMLYDGHQEGYVLTTLKRTKIRGVESYSMICSEKELGIS
                     DEHEGIMFLDDAASVGTPLVELLGDAILEVNLLPSFARCASIFGLAREISAITGKALK
                     TPRFTSPPDIGKATYAELEITDPVLNPRFVLGLVRGVNIHPSPLWVQQRLKLAGMRPI
                     NNIVDATNYAMLELGEPLHAFDYDVLCKRAAGKQVKIITRAAGKDEKITTLDGVERTL
                     DEGTVLVCDEAGILSLAGVMGGLETEVTASTRNILLEGAAWNMISTRRTAKAQNLPSE
                     ASYRFIRGVHPAMAETGVRRGLELMEHWADGAVDPLMVDAYPLPPKTSVVDITPGDVK
                     RWLGLDLSAPRIAKLLSRLEFKVQIDSDLLHITCPEHRLDIGSGITGKADLIEEIARV
                     HGYDNIPETRISDRLPLQLGNSPLKKTDLTRDLLVALGLQEVISYRWTTPEKEGRRLP
                     KGAEADNKPYLRISNPLAYEKAFLRHSVLASLLDTAERNSRQRATLALFEIGPIFLHS
                     EDPSGLPDELSRLGILLAGQRNPSGWQPADNSTMDFYDLKGLLEDLMAGLHITEANFS
                     QGEHPGYHPGKCAIIRVGSQQVGVCGEIHPAVRERYHWGDTFQYPVLAAELDMDLLIE
                     LIPEQRHTTDIPNFPAVVEDLAFVLDEATPAADVTALIWQTGGKILSELQLFDVFRGG
                     QLGSGKKSLAYRLTYQAVDRTLTDKETAQVRNRIIKRLEMELGARLRS"
     gene            complement(3306..4265)
                     /locus_tag="P5516_01825"
     CDS             complement(3306..4265)
                     /locus_tag="P5516_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HRT91208.1"
                     /translation="MHYRRLGQSGLKVSEISLGAWITFGNQIDEKTAISLIKEAFDQG
                     INFFDNADMYANGQAELVMGKAIADMPREELVISSKVFWPTMKGPNGRGLSRKHITES
                     IHASLKRLGTEYVDLYFCHRYDPDTPVEEVVRTMTDLVHQGKILYWGTSEWEASQIMQ
                     AYGAARQFGLIPPSMDQPQYNLFHRKRVEDEVMPVARELGLGLTTFSPLYYGVLSGKY
                     NNGIPKGSRASLEDYGWIRDRITPERIQVVKCLTEIASDLDITPAQLAIAWVLRRKEV
                     SSVITGATRLEQLDENLGASLAYERFTELDIDRIFACLGAHQE"
     gene            complement(4266..>4700)
                     /locus_tag="P5516_01830"
     CDS             complement(4266..>4700)
                     /locus_tag="P5516_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRT91209.1"
                     /translation="HFRTPVAKGIEIIEGLRGHTSGFAVPTFVVDAPGGGGKIPVMPN
                     YHLSSSDHKVILRNYEGYITTYEEPQDYKAHDPKTCKYCQNKRSEPGQSGVSGLLDGE
                     ELAIKPENFDLLHNRGGGMHRLRADERKWKPLGIGAGQKEDK"
     assembly_gap    4701..4800
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4801..5254)
                     /locus_tag="P5516_01835"
     CDS             complement(<4801..5254)
                     /locus_tag="P5516_01835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine 2,3-aminomutase"
                     /protein_id="HRT91210.1"
                     /translation="MTPTPFLSKRAPFFKDVPDEQWNDWRWQLSRRLNAVEDFDKIFT
                     LTESERQALSTDGLFRVDVTPYYVSLIDPDDPHDPIRRQIIPTAGEINAFTGMMEDSL
                     AEDRHSPVPGLVHRYPDRVLMLVTTQCASYCRYCTRARIVGDPNQTFSR"
     gene            complement(5390..7315)
                     /locus_tag="P5516_01840"
     CDS             complement(5390..7315)
                     /locus_tag="P5516_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRT91211.1"
                     /translation="MEEPINIFEQSSGIPREKKLQTWLIGRPLATADAPHQTIGKLVG
                     LAVFSSDAMSSVAYGPQELMLILAVAGAGALHLALPLVVGIAVLLIILTFSYEQTIHA
                     YTNGGGAYIVARDNLGELPAQVAGAALLTDYILTVAVSISSGVAQLVSAYPGLNSFRV
                     LIAVGLIFFVTIINLRGVKESGITFAIPTYFFLVLMLITVVAGFARYFSGTLGIVVEP
                     PLLEHEYLQPVTIFLLLKAFANGTTSLTGVEAITNGITAFKEPRAKNAGKTLLLMSGI
                     LGVLLLSITFLSTKVGTIPSDSETIVSQLARTIYGSRGILYLLVISSTTIILIMAANT
                     AFADFPRLGALQAADGFLPRQLTFRGSRLVYSSGIVLLAVTASLLVIAFRARVTGLIP
                     LYAIGVFLSFTLSQTGMARRWWKAGHLKPGDEVVESGSTLRYDKGWVHKMVINGFGAF
                     CTSIVAIIFAVTKFIDGAWIIIILVPLLVTIFFQIHRHYKKMARQLSLENPHTSRMIT
                     RNRVIMPISGVHNGTLAGLQFAKSLSDDVTVVHVSIDPAETAKVKSKWERWGEGYRLV
                     ILDSPYRLFLEPLLAYIEKISLILAPNEIITLVVPQFVPRHWWVNFLHMRAAETLRKA
                     LLHSKKIVITEVPYQVD"
     gene            complement(7449..8144)
                     /locus_tag="P5516_01845"
     CDS             complement(7449..8144)
                     /locus_tag="P5516_01845"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT91212.1"
                     /translation="MDKDPLILLIDDEPQILRALKTILSSGHFRVISAVTGEEGIALA
                     ASQLPDVIILDLTLPDMDGISVCEQVREWSTIPIIVLSVRDGERDKVAALDKGADDYL
                     TKPFNIEELLARIRVALRHSSQSIGNRETVIRAGRLTIDLARHLVSLDGAELKLTATE
                     FKLLAYMASHADRVLTHQAILTHVWGFEEADRLEYLRVYIGQLRKKIEVDPDAPVVLV
                     TDPGVGYRFKTGE"
     gene            complement(8137..>9207)
                     /locus_tag="P5516_01850"
     CDS             complement(8137..>9207)
                     /locus_tag="P5516_01850"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT91213.1"
                     /translation="SSLAGVTDIRETIRLLATRLFDTFHFDQIEISTWDLSSNELIIY
                     QYPETARLETPADLVIHMSTARNDEGTLNLWFDQNKLTVEQTRLLSAFCNQGALAIER
                     VRLFRSENLAKVLEESDRVKTALLNSVSHELRSPLAAIKASVTSLRGGAVPWETSARA
                     ELLATIDEETDALNLLVGNLLDMSRIEAGTLKPTKCWNSLIEIIKGVEEKMRRQIGEH
                     RIKYLVENELPLVPTDYVMMGQVFTNLFSNSNKYAPENTPILVKIGVAAGQMLVKVIN
                     QSPPVPEEHLEHIFEKFFRISETDKVTGTGLGLPICKGIVEAHEGKIWAENTADGFTF
                     NITLPLTLDGALPRLPKDVIDG"
     assembly_gap    9208..9307
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            9423..10460
                     /gene="hrcA"
                     /locus_tag="P5516_01855"
     CDS             9423..10460
                     /gene="hrcA"
                     /locus_tag="P5516_01855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HRT91214.1"
                     /translation="MTELTDRQRMLLSLVIHEYVRSANPVGSKHVVEQFNLDMSSATV
                     RNELSTLTELGYLRQPHLSAGRVPTEDGYRLFVGNIVQETELPDSTRRTIAHQFYQMR
                     QDVEGWTRLAASILAHQSRAASLVTAPHPEQSRFKHLELIAARGRQVLMVLVLMGGEI
                     MQRFITLPEPVSQEQLSSAADRLNLIFSGQNIGEMSSDSSELVALEQEFYQIVLDEME
                     QADAMISGEVVLDGFTNVLAEPEFADSEEARRALRVLEEKTLLNDILSKTVLHGSSLG
                     GVQVLIGGEGIYDALSQCSVVLARYGTPGLATGTLGVLGPMRMPYGRTISIVRFLSGL
                     LSDLVNDTIIE"
     assembly_gap    10561..10660
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <10661..10918
                     /gene="grpE"
                     /locus_tag="P5516_01860"
     CDS             <10661..10918
                     /gene="grpE"
                     /locus_tag="P5516_01860"
                     /inference="COORDINATES: protein motif:HMM:NF013212.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - chaperone binding [Evidence
                     IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HRT91215.1"
                     /translation="KPNGGHGEAWATGIELIARKLLSIIEAEGVERIWQNRVPFDPTI
                     HEAISSEENPDFESGDVIEVTRQGYKLGDRVLRPAMVRVAK"
     gene            10940..12868
                     /gene="dnaK"
                     /locus_tag="P5516_01865"
     CDS             10940..12868
                     /gene="dnaK"
                     /locus_tag="P5516_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRT91216.1"
                     /translation="MGKIIGIDLGTTNSVVAVMSGGEPVVIPSAEGERLIPSVVAVNK
                     NHERIVGRAARNQAIVNPENTIFSIKRLMGRKFSDPEVQRTISRVPYKISAASNGDIR
                     VTLDGKEYSPPEISAMILSKLKADAEAYLGETVNQAVITVPAYFNDAQRNATKDAGRI
                     AGLEVLRIINEPTASSLAYGLDKKKNEVIAVYDLGGGTFDISVLDVGEGVFQVRSTNG
                     DTFLGGDDFDLRVMDYLIEEFKKSSGVDVHNDRQALQRLKEAAEKAKIELSTVQQTEI
                     NLPYLTADANGPKHLVLTLTRAKLEQLTGDLIQKTMEPIRRALSDASLKANEVNEVVL
                     VGGMIRMPAVQDAVKREFGREPHKGVNPDEVVAIGAAIQAGVLGGDVKDILLLDVTPL
                     TLSVETLGGVSTPLIERNTTVPTRKSQVFSTASDSQTQVEIHVLQGERPMAADNKSLG
                     KFNLDGIPPAPRGVPQIEVTFDIDANGILNVTALDKATNRSQHITITASSGLTESEIQ
                     QMKRDAEANAEADRKQKELVEVRNNADNTIYAAEKLLRENSEKIPADTKKEVEEAIAS
                     LRGLMNNEDAGAIRTATEALGQSIQKIGASMYQQQPEAGGQGAPGEQQGQPGTDSNGG
                     NPPDGDAGDVVDGEFKSV"
     gene            12967..14082
                     /gene="dnaJ"
                     /locus_tag="P5516_01870"
     CDS             12967..14082
                     /gene="dnaJ"
                     /locus_tag="P5516_01870"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HRT91217.1"
                     /translation="MTKRDYYEVLGVNRNASAEELKSAFRTLARKYHPDMNKEADSEE
                     KFKEINEAYAVLSDPDKRRAYDQFGFAGVNGAGGVPDWTTMDFADIFGEFFGFATGAS
                     RARQNAPRRGQDLAYTVTLTFEEAIFGVEKEVEITRDEVCHTCGGKGAEPGTNPAKCS
                     NCAGRGEVRQVRQTFLGSMVQVTTCPVCAGRGEIIGTPCHTCNGRGLERKTLSKKVAI
                     PGGVDNGTQIRLAGEGQPGINGGPTGNLYIEIKVKSHKFFRRKENDILLDLNINIAQA
                     ALGADVEIPTVDGPTKLVIPAGTQPGKVFTLRGKGSPILRSNARGDQKVIVNVEIPTR
                     LTDEQRQHLQALAASMGTEVKPQEKGFLDALKEVFGG"
     gene            14075..14995
                     /gene="prmA"
                     /locus_tag="P5516_01875"
     CDS             14075..14995
                     /gene="prmA"
                     /locus_tag="P5516_01875"
                     /inference="COORDINATES: protein motif:HMM:NF018076.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HRT91218.1"
                     /translation="MAEGKWLQVSLTVDGELAEAVAEVLNRFTSGGVVVESDVIYRNA
                     EDEGTPYGAVRVFGYLLIDENIEVNRHKLEEALWHLRAIRELPQPEYREIADENWMEA
                     WKAHYHPIPIGKKLLILPAWIKQKDQSRLAVKIDPSMAFGTGTHPSTQLCLELLEDYT
                     APGKPVIDIGCGSGILSIAALKLGASKAIGVDIDAPAIKATHENSRANGVEEKVETGL
                     GSVEEVKRGDFSARQAPLVLANILAPVLIHLFEVGMAQLVSPGGVILLAGILSEQGAS
                     VRAAGEARGLTFVEERCCGDWSALVMRQPQ"
     gene            complement(15102..15617)
                     /locus_tag="P5516_01880"
     CDS             complement(15102..15617)
                     /locus_tag="P5516_01880"
                     /inference="COORDINATES: protein motif:HMM:NF016840.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRT91219.1"
                     /translation="MIHPLVAQLFFTRSEFERGLEGVSAEDAVKHLGPMNCISWIIGH
                     MTWHEQKIWHDLLHGKTHYPDFVNRFAHGAPKSSPPLEDVLNAWHAVTRATTAELEKL
                     TTADLQADLLHLGKPVGQNLGSAMLRVIYHYWYHIGEIQSIRQILGHRNLAEFVGDLD
                     AKAPYRSEYSG"
     gene            complement(15740..16144)
                     /locus_tag="P5516_01885"
     CDS             complement(15740..16144)
                     /locus_tag="P5516_01885"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRT91220.1"
                     /translation="MSSASVKWIGGKQFVGMDSTRHTVVLSTPDEGVGMKPSELMLVA
                     LASCSAVDVVEILNKKRAGLTSLEISAEAEQEQDPPWTFRKIHLTYRMKGSALTEKDA
                     AQAIELSEGKYCSVAATLKGKAEITTSLIILE"
     gene            complement(16161..16433)
                     /locus_tag="P5516_01890"
     CDS             complement(16161..16433)
                     /locus_tag="P5516_01890"
                     /inference="COORDINATES: protein motif:HMM:NF014624.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HRT91221.1"
                     /translation="MKVTSENGKKQVINRLNRLEGQIRGINRMLAQERDCSDILQQFV
                     AARSALQAALDVYTGQIVNDCLLADDLSLEQHRKLAVEMLAVIRKT"
     gene            complement(16467..16676)
                     /locus_tag="P5516_01895"
     CDS             complement(16467..16676)
                     /locus_tag="P5516_01895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT91222.1"
                     /translation="MPLYEYICADCGHEFEKRMRFDQAGERPPCPRCAGKDTRKKLSL
                     FSSAGNASGGAVSTGSCGSGSGGFT"
     gene            16844..17569
                     /gene="lexA"
                     /locus_tag="P5516_01900"
     CDS             16844..17569
                     /gene="lexA"
                     /locus_tag="P5516_01900"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HRT91223.1"
                     /translation="MARRQTGLSERHRKIMEFLTRFQDSHGYSPSIRQIGDSINVKST
                     SLVDYYLNQLQNLGYIEREDRISRSIRVLQPIQPGVSLPARVGEAFRGAASALASMVS
                     IPIAGRIVASEPIPMPVSDLNYYDAESSVDIARSLLPAKDVNDLFALEVSGDSMIDAM
                     INDGDIVVMKKASSANNGEMVAIWLDDNNETTLKYFYKEADRIRLQPANPNMEPIFVK
                     NPKSLRIMGKVVMVIRQVHSVAG"
     assembly_gap    17990..18089
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<18090..18292)
                     /locus_tag="P5516_01905"
     CDS             complement(<18090..18292)
                     /locus_tag="P5516_01905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91224.1"
                     /translation="MSRKIIPLLVILALLTLSCSRSLAGKTGSTPVVVPIGTDFLKAT
                     VEAMRPPTITPTPTHDITLGTVTP"
     gene            complement(18428..19276)
                     /locus_tag="P5516_01910"
     CDS             complement(18428..19276)
                     /locus_tag="P5516_01910"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRT91225.1"
                     /translation="MIGIITDSTCDIPDALIEQYGITIIPHYIIWGDEQFRDRVDMTP
                     LEFYKRLEVDPVYPSSSQAGIPDFQKTYEEVAAKGATEIITLTVSSAMSGAYHMACEA
                     ARLVKIPVSVVDGKGPTMSLGWQVLAAARARDAGMEVKKILTMLDDLKKKLVQYVAMD
                     SLEYLQKSGRIGNAVKWVGSLLKIQPLVSINHHSGLVEPAAMARTHKQLVEMLYQKFF
                     AQLRSHNRLHIAVLHGNSLAEAEALAERIRPEFNPVELILNITGPVLGISTGPGALAL
                     CGYSEE"
     gene            complement(19367..19528)
                     /locus_tag="P5516_01915"
     CDS             complement(19367..19528)
                     /locus_tag="P5516_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91226.1"
                     /translation="MMPDTSVYMVAGFLVIIGGVAIYALWLLTRFNDCAKRINTLKEL
                     EKGKRPYGE"
     gene            complement(19525..20238)
                     /gene="ccsA"
                     /locus_tag="P5516_01920"
     CDS             complement(19525..20238)
                     /gene="ccsA"
                     /locus_tag="P5516_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HRT91227.1"
                     /translation="MKPEKTPRALIFLDFLALLALATAFALVIFYAPFELTMGSVQKV
                     FYFHVAAGLAGMLGFFVATIAAVACLKTGSLKWDVAGLAGVEIGMVLMALCIVSGSIW
                     ARPVWNTWWTWDPRLTTALIMELIYAAYLLLRSGLDDARRRAKLAAVYAVVGFISVPL
                     TFFSIRVFRTIHPVMIGSADSSANGLFAMNPSMQTAFFSSLAAFSILFIALYWHRYRL
                     GNLQAEINERRAALQEEGE"
     gene            complement(20251..20925)
                     /locus_tag="P5516_01925"
     CDS             complement(20251..20925)
                     /locus_tag="P5516_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HRT91228.1"
                     /translation="MKPFIGAILAIFWKDLLAERRTREILGTMLVFALTVILVFVFAF
                     DLSVEIRRKAAAGVIWVTLCFAGTISLNRSLTLERDREAINALLLAPVDRTAIFFGKA
                     LVNWVYLLITAAIIVPVYALLNNLSLFSWGGLGVILLGTLGYILTGTLLSTLTLQLRA
                     RDMLLPVLLFPVIIPLLLACVRASTILLQGGNPAELDTWLVLLVGYDLLFAAVGLLVF
                     DRIIEE"
     gene            complement(20922..21653)
                     /gene="ccmA"
                     /locus_tag="P5516_01930"
     CDS             complement(20922..21653)
                     /gene="ccmA"
                     /locus_tag="P5516_01930"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HRT91229.1"
                     /translation="MLNAVNLTRSFGNRQVLRGLSLTVEPAEIVSLVGINGAGKTTLL
                     RVLANLLRPEQGMVRFGSYTPLNAPAEYRRHIGVVMHTSMLYDNLSARENLRFFSRLY
                     QIADSDKRIRQVLEMVALNSRADELVRTFSRGMQQRLAIARALLHDPDLLLLDEPYTG
                     LDQRSSELCEMLIRQAAADGKMVLLTTHDFNQAVAISTRVDLLHDGKISWSSPSAGLS
                     GKSLRATFNELTVFSGSHKGHGVNA"
     gene            complement(21670..21969)
                     /locus_tag="P5516_01935"
     CDS             complement(21670..21969)
                     /locus_tag="P5516_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91230.1"
                     /translation="MMLIPIVITVILLALVFFYITRPLFTHQPLGGSTYAPDVKALKQ
                     AAYQAILERIRELDFDFGLGKMSQQEHEEQRTLLLQEAAVALRELKMVDSPPGSA"
     gene            complement(21985..22518)
                     /locus_tag="P5516_01940"
     CDS             complement(21985..22518)
                     /locus_tag="P5516_01940"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HRT91231.1"
                     /translation="MNKAKKRFPYWMVIFGLLLVGLLITLGLWLKQSRQTGFEVGAEI
                     PDFSITTFSGEHYQKSRMDGRVILLNFWSSWCASCEEEGAILEEVWREVKDSDDIIFL
                     GVNYVDTENDAKAFLEKYGITFPNGPDMGSRISRLFKVQAVPETYLVDKDGRLAAVQI
                     GPFQSADEIRAMLELAK"
     gene            complement(22548..23027)
                     /locus_tag="P5516_01945"
     CDS             complement(22548..23027)
                     /locus_tag="P5516_01945"
                     /inference="COORDINATES: protein motif:HMM:NF015853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein CcmH"
                     /protein_id="HRT91232.1"
                     /translation="MKRVLLVIWLFFLLIVQTPGYAQTEPPVSDDEVNRLASELYCPV
                     CENVPLEVCPTKACAQWRELIREKLALGWNDEEIKAYFATQYGDQVLAVPPRRGFNWL
                     IYLVPPLTLVAGILIAIRVIRKPKTIPAAQEVSNEGPCRMAAEDFISQIENDLEGVE"
     gene            complement(23024..25063)
                     /locus_tag="P5516_01950"
     CDS             complement(23024..25063)
                     /locus_tag="P5516_01950"
                     /inference="COORDINATES: protein motif:HMM:NF027651.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HRT91233.1"
                     /translation="MIGGIGYGGLVIALVTTLTAVITSLCGGRSRSDALIETARRAAL
                     ITFPVLSLSILALLYLLASGRFEYSSVYQTTDLSMPLYLKLAALWGGQEGSLLFWCWL
                     LAGFTFPVVARRWKNEADLLPWVIAATSFTLLFFIILVLFFENPFLRYFITLDGIIKR
                     AFFPPAMSILAKPTDGTGLNPLLRHFGMIIHPPALYLGFTGFVIPFAYAFAALVSGRT
                     DDHWLKSTRKWALLAWLFLSLGLVLGSRWAYDVLGWGGYWGWDPVEISALMPWLTGTA
                     YLHSAITQEKRRLFQHWNIALIVLTFCLVILGTFLTRSGMLSSVHAFSQSAIGPAFFV
                     FIILMFSISFGLLLYRWSALRSENQIHSVFSRESAFLFNNLLFTALLLICLTGVLFPI
                     FSELLTGQKVTVGPPFYESATAPLFGLLLLLMAIVPLTMWGVSTSKNLERHLWKPALA
                     ALAVPLIVFLAGVRNWFALLAVWLVTLAIFTTVLDTYRTVTLRASNRQEPVRQAFGTL
                     VRRFSRRYGGYLIHIGVALMALGIIGIEFFQTTTQGMLAAGETLSLSGYTFTYEKLEV
                     TDNADGRNSARASVMIKRDNRELKRVFPGSDTYYVSQQSVTLPGVRSTLVDDLYVILV
                     DWQPISSEGATFKIYRNPLINWLWIGSGVLVFGTLVALWPTTRRKNIPRTHQEEK"
     gene            complement(25060..25575)
                     /locus_tag="P5516_01955"
     CDS             complement(25060..25575)
                     /locus_tag="P5516_01955"
                     /inference="COORDINATES: protein motif:HMM:NF015085.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HRT91234.1"
                     /translation="MNKSPRAVEPHPRFKIHKFAIGGMLLLAAVVLLVITSLKGNAQY
                     YLTVEELASGKGVNNANVRVSGVVLGDTIQYDPQTLDLTFTIAHIPGDNREIEAGGGL
                     ANVLHNASLDPQAARLQVRYNGVMPDLLKNEAQAILTGAMGDDGVFNAEELLLKCPSR
                     YEAEVPAQAVP"
     gene            complement(25642..26028)
                     /locus_tag="P5516_01960"
     CDS             complement(25642..26028)
                     /locus_tag="P5516_01960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91235.1"
                     /translation="MKFFNLGLPEIIFIVVLALILLGPGNMVKSAREVGAFIRRITRS
                     PYWQEVWATRREINEIPRMLAREANLDETIRDLDRQSRGVQSSLTRSVADLIKEVEEP
                     RKDNQADINRESPDLHEAPEPPAERE"
     gene            complement(26077..26253)
                     /gene="tatA"
                     /locus_tag="P5516_01965"
     CDS             complement(26077..26253)
                     /gene="tatA"
                     /locus_tag="P5516_01965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HRT91236.1"
                     /translation="MFSKLGVPELIIILVIIALLFGVGKISKIGGEIGGAIRAFRKGL
                     KGEEKEEKDDSTQE"
     gene            complement(26309..27055)
                     /gene="tatC"
                     /locus_tag="P5516_01970"
     CDS             complement(26309..27055)
                     /gene="tatC"
                     /locus_tag="P5516_01970"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HRT91237.1"
                     /translation="MEQETSGMSILSHVNELRKRLLISVIALAVATVIGFVFSRQLAE
                     VLAGPIGGLSAMKSIDVTENVTAFMKISLLAGVVLALPIVVYEIFAFVLPGLKPNERK
                     WVWFMIPLATLFFAAGVVFAYFVLLPTALPFLLNFMGIATAPRPSTYFGFVLNLLFWV
                     GLAFELPLIVFLLARIGLVTAKQLARQWRIAIVIIAILAAFITPTPDPVNMGLMMIPL
                     CALYLISILFAVAAGHNRKKSQQADGAGAG"
     gene            complement(27309..28103)
                     /locus_tag="P5516_01975"
     CDS             complement(27309..28103)
                     /locus_tag="P5516_01975"
                     /inference="COORDINATES: protein motif:HMM:NF019533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HRT91238.1"
                     /translation="MIQRERRKREQNLLQSPLNWFSLVGLYPLQTGENRLGHGSGYTI
                     HIPELAQGAEALITLTGDEIWVEVGSHFTINGAPAAERQLRTDNDENPDLLECASLAM
                     RVIQRGRLYFLRVWDRDSQRLKSFHGLNYFAVNPAWRVSAEFIFFDPPRETIVHTAVG
                     SEMRVSIPGSARFQMSGVNCTLLGQAEGDELLFSFTDLTRQDATYPGGRYLVTRRPVH
                     NRLILDFNLAWNWPCAYTAYATCPLPPPENHLAMRVEAGEKRYHDE"
     gene            complement(28344..28829)
                     /locus_tag="P5516_01980"
     CDS             complement(28344..28829)
                     /locus_tag="P5516_01980"
                     /inference="COORDINATES: protein motif:HMM:NF023621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3597 domain-containing protein"
                     /protein_id="HRT91239.1"
                     /translation="MGVFSKILDKLGIKGKEEKAAAAAPAVKPEIPKTSALKPGGSVA
                     KDSRPVAGMVGAAGSGFKLDAFKPGAPAAVPMVDVVAKLEKLAAANPEKLDWKTSIVD
                     LMKLLGLDSSLAERKELAKELGCPEEKMADSAQMNMWLHKVVLQKIADNGGNVPKELL
                     D"
     gene            29365..30516
                     /gene="nagA"
                     /locus_tag="P5516_01985"
     CDS             29365..30516
                     /gene="nagA"
                     /locus_tag="P5516_01985"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HRT91240.1"
                     /translation="MMKTHIRNAILLTPQQRMEHTVLTLSGGVIEKLTPEEEFSFEPG
                     NTLDAKGCYVAPGFIDLHFHGAMGVDTMDAVAAGMVTASAYCSQHGVTTFYPTTWSAS
                     QAEITRVINCVKECASHLPGARVGGVHLEGPYINPRRRGAQSLSFIRKPDAREYLPWL
                     ESGMVSILTCAPEVEGGEALIRNAVRLGARVAIGHSSASYEQVKAAANLGATQAAHLF
                     NGMDGLHHRHPGTAGGILDDDRLLAQVICDGVHLHPAVVRLVLKAKSPSRIALITDSI
                     RGAGRADAKYEGNGQFIQVKDGVARDAEGHLSGSTISMDLALRSLIAFTGCDLLEALV
                     MVTSAPAEEMGLGAKKGRLAPGYDADLVFLQKDLTVAATMVSGAFVYQC"
     gene            complement(30606..31628)
                     /gene="iolG"
                     /locus_tag="P5516_01990"
     CDS             complement(30606..31628)
                     /gene="iolG"
                     /locus_tag="P5516_01990"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HRT91241.1"
                     /translation="MSNQKVIRVGVIGAGRIGKIHAENLATRIPGVVVSAIADINQQA
                     AQALAEKLHVANAFSDYRKIIEDKDIDAVAVCSSTDTHADLIIAAANAGKHVFCEKPV
                     DHSLDRIDKAINAVSKAGVQCQIGFNRRFDPNFKKLHDLVQTGKIGDLHILRITSRDP
                     APPPAEYVKLSGGMFLDMTIHDFDMARFLSGSEVEEVYAIGEVRVDPAIGAAGDIDTA
                     VITLKFANGAIGTIDNSRKAVYGYDQRAEVFGSGGMAATANNTPNSSVYSNADGVVSE
                     KPLYFFLERYMESFVAEMRDFINAIREGNPTPVNVFDGRKPVVIAMAATRSMLENRPV
                     KLSEIE"
     gene            complement(31641..32543)
                     /locus_tag="P5516_01995"
     CDS             complement(31641..32543)
                     /locus_tag="P5516_01995"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HRT91242.1"
                     /translation="MIRIANAPCSWGALEFDLQGKAAGYERVLDEMQRSGYAGTELGD
                     WGFMPTEPGKLKEELKKRELVLLGAFVPVALKDATTHPQGIESALKVARLLAAVEGSS
                     PFIVLADDNGKVPERTRNAGRVTPEMGLSDAEWQVFAEGTEKVADAVKRETGLRLVFH
                     HHCGGYVETPAEIDSLLRLTDPQLVGLCLDTGHYQFGGGDPLTFLQENAGRVWHMHFK
                     DCHPGIAERSRVLGWDYFKSVEQGVFCELGKGAVDFPAVKAELERQGFEGWIVVEQDV
                     LPGMGSPYESALRNREYLKSIGFN"
     gene            complement(32570..33586)
                     /gene="iolG"
                     /locus_tag="P5516_02000"
     CDS             complement(32570..33586)
                     /gene="iolG"
                     /locus_tag="P5516_02000"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR04380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HRT91243.1"
                     /translation="MAVKIGLIGAGRMGRVYASTLAFSVSEVDLVAVADPDRAALAEV
                     ADRYHIPSTYQDYRDLLDSQEIEAVVVASPTGTHAEVIAAAAQAGKHIFSEKPLSQNL
                     QRCDDALTAVRQAGVKLQMGFMRRFDAPYAAAKQKIRDGVIGKPVMFKSTSRDPKRTS
                     LEFAKRENSGGLILDMGVHDFDLARWLMDSEVARVHSEGACLAFPELKEVGDIDNVVI
                     NLRFTNDAIGNIDLSRNAVYGYDIRTEVLGTDGSLWIGYLQQTPTLVLTRAGVMHDTV
                     PYFMERFGLAYAEQVRVFVKHILEDTPPEVTGEDARAATAIGIAATLSLDEGRPVQVR
                     EVDL"
     gene            complement(33617..35482)
                     /gene="iolD"
                     /locus_tag="P5516_02005"
     CDS             complement(33617..35482)
                     /gene="iolD"
                     /locus_tag="P5516_02005"
                     /EC_number="3.7.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR04377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016823 - hydrolase activity, acting on
                     acid carbon-carbon bonds, in ketonic substances [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3D-(3,5/4)-trihydroxycyclohexane-1,2-dione
                     acylhydrolase (decyclizing)"
                     /protein_id="HRT91244.1"
                     /translation="MTSTIRLTMAQALVRFLKNQYIERDGVETPFFAGMLGIFGHGNV
                     AGIGQALQENPDFRYIMVRNEQSAVHIASGFTKMHNRLQTFACTSSIGPGATNMVTGA
                     ALATINRLPVLLLPGDIFARRNVAPVLQQLESPNTQDISVNDCFKPVSRYWDRITRPD
                     QLLTSLPEALRVLTSPADTGAVTLSLPQDVQAEAYDYPLEFFRKRVWMVARPLADRAL
                     LKRAVEMIRSSKQPLIVAGGGVLFSGASQALDTFARQTGIPVGETQAGKGSLAYDHPQ
                     CLGAIGATGTLPANRIAHGADLVIGIGTRYSDFTTASKTAFQNLAVRFININLFEMDA
                     FKHSGLALTADARATLEELTSALAGYQVSADYATRIRELKHEWEVETDRLYHLRHQPL
                     PAQSEIIGAIWETIADGDVMMSAAGSQPGDLHKLWRARTPNSYHMEYGYSCMGYEIPA
                     SLGVKLAAPEREVYVWVGDGTYLMNPTEILTAAQEGLKVTIIVVDNAGFGSIGGLSAS
                     LGSSGFGTRFRKRDPQTGQLNGPALEVDFAANAASLGAEVFTPGSIEDFRKALAHTRS
                     LDHTSVIVIKADREVRVPGYESWWDVAVAEVSTMPEVQAARKDYEEHLKDEKYFL"
     gene            complement(35501..36580)
                     /locus_tag="P5516_02010"
     CDS             complement(35501..36580)
                     /locus_tag="P5516_02010"
                     /inference="COORDINATES: protein motif:HMM:NF013116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HRT91245.1"
                     /translation="MTTYKSKLHETVSTTPTDYWNDSCSIEELTYAVEHGAVGATTNP
                     TIVANVLKKEMNLWEERIHQIIAENPIWSEVEITWKLIEEMAVRGAGLLKPAFDRERG
                     KKGRLSIQTNPQLYRNARGIAEHAIHFATLAPNMQVKAPVTSAGVKAIEEATYHGVSI
                     NATVCFSVSQALAVAQAMERGLERRSAEGKSIAEMSPVCTIMVGRTDDWMKVAAKKNA
                     VDIDPAFLDWAGIACFKRAYEIFKEKGYRPRLLAAAYRHLGHWSEFIGGDVILTIPYE
                     WQLKINASDIEVRPRMADPVDPVIVKALLSKIPEFRKAYEPDGMTVAQFDTYGATVRT
                     LRGFISSYHELQGVVRDFMLPNPDL"
     gene            complement(36592..37470)
                     /gene="iolB"
                     /locus_tag="P5516_02015"
     CDS             complement(36592..37470)
                     /gene="iolB"
                     /locus_tag="P5516_02015"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: protein motif:HMM:NF016826.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HRT91246.1"
                     /translation="MKSTESSLLVKPDLTRAEYIRVTPQSVGWEHLSFCAVRLKQGEE
                     WHFNTENNELALVILGGTLDVHSNRGAWRSIGSRTDVFHGMPTTLYLSRDTDFTVTAA
                     YGAVDFACGWAAATKDFPARLVSPEEVKVELRGGENASRQINQMILPGFPCERLVVVE
                     VYTPSGNWSSYPPHKHDVRRVDENGRLLEADLEEIYFYKIDKPEGYAYQRCYTDDRRI
                     DEIMLVQNDHLVLSPEGYHPVVAAHGYTAYYLNILAGSDQSLASSDDPAYAWIKDTWK
                     SKDPRLPLVQLSMNYK"
     gene            complement(37474..38502)
                     /locus_tag="P5516_02020"
     CDS             complement(37474..38502)
                     /locus_tag="P5516_02020"
                     /inference="COORDINATES: protein motif:HMM:NF012742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HRT91247.1"
                     /translation="MGVTIKDIAKVAGVSHTTVSRALNGNPVIPPKTAAMIRNIAGDL
                     GYLPSAAARGLKTRRSGVLGVLVSRIDNPYFGEIIQGIEDAIRNSGYSIFIASSSLDP
                     QKEKEIVQAFSEHRVDGVIIGSVPVERSNLNLLNQYGIPAVVINNQSARSQKYSISHD
                     DEYGARQVTRHLLDLGHRRIAYLGNACAPRINDNRLQGLKAECESSGFTVAENWITHQ
                     PGGEIENGAAGMTALLGAGVPFSAVFCFNDLMAVGALSVLRQNGLRVPEDISIAGFDN
                     IPYSAYTTPALTTFDQPKRSIGDEAASMLLELISSNASSQSRRKPLNKMMRGELLVRK
                     STAQFAKE"
     gene            38738..39481
                     /locus_tag="P5516_02025"
     CDS             38738..39481
                     /locus_tag="P5516_02025"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRT91248.1"
                     /translation="MNPDIEAIFLDVGNTLRIVLEEPDFQQNAMQELMRLVGTQESQE
                     AFFEKLETRWKAYRKASKASLLESPEEILWTKHLLPDYPAEKIAPLHGRLTRLWRDRD
                     GRRVPRHDAKETIIELHRRGYLLGIIANTITETEIPDWMEADGVTQYFKTVILSSKVG
                     IRKPDPEIYWMAARQIGVEPAKCAYLGDNPVRDVEGTRAAGFGMMILFDEPATLAKEP
                     PTGEHTPDYTIKEIRELLDIFPPRNRQVN"
     gene            39491..40225
                     /locus_tag="P5516_02030"
     CDS             39491..40225
                     /locus_tag="P5516_02030"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRT91249.1"
                     /translation="MDIDAIFLDIGNTLRILMKEEPYREEARQKIAALVGTSQPPQSF
                     SEELDRRYKEYRKWAFEHLVEASEEELWTRWLAPEFPAQHIAPLAHELTYQFRQSMGR
                     RVMADGCKEVIHELHSRGYTLGIISNVITTSEIPDWLAAENLSRYFKSVLLSSTFGRR
                     KPDPSIYLEAARRAEVAPAKCAYVGDNFSRDVAGTRAAGFGMVIILPDPAEKDLRVAP
                     EQQPDLVIHSLWDLLDHFPPRNGHQE"
     gene            40228..41835
                     /locus_tag="P5516_02035"
     CDS             40228..41835
                     /locus_tag="P5516_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91250.1"
                     /translation="MPKSAGISLENTNRARGSAALAKTVKDLYERGQTDYNMEPIILQ
                     DAAGNAVGRIKDGDAVVFCCRRGEREIQLTEAFTDPQLNQFPREVFTHLPFVILTLYH
                     DKFKDLPVAFAPSKVQATLGEVVSQAGFSQVRIAESEKFAHVTFFLNGGNNQPYPQEE
                     DIRIPSLKGVPFDQYPELSLEKVAQAVIEAIHRGTGLVVTNFANGDVIGHTSNKAAKI
                     ACAQFVDRHLREVVEKANAAGYVVIITADHGNLEVLENADGSAHVSHTTNPVSCLIID
                     PAAAETVTPREGKLADIAPTVLSILRVTPPAQMTGKNLAPEHDFGVDRKVLLVILDGW
                     GISGVPENDPIAMAETPFWDSLLARYPHSLLEASGEAVGLKAGKAGNSEAGHINIGTG
                     RVVLQDDVRLDRAMQDGSFYTNEVICDVIEGVKARGKNLHLIGLLTEKSSHGSMEYPL
                     AVLKMAKDAGLQHVFLHMILDGRSTEPGSAPALLKKLEDLMNEIGVGQIVTGIGRGIA
                     LDRDGNYNKTQIAYDALVYGKGRGAGK"
     gene            41832..>42751
                     /locus_tag="P5516_02040"
     CDS             41832..>42751
                     /locus_tag="P5516_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91251.1"
                     /translation="MNYEFLKKLIQPADTKIVMLIMDGLGGLPKEPGGKTELETAHTP
                     HMDALAARSALGFSSPAGAGIAVGSGPGHLAIFGFDPIEHEIGRGALEALGVDIDLGA
                     EDVAARGNFCKVDANGILTDRRAGRLATPEAFRLAKMLNSIAIDGVQIQVEPIKEHRF
                     ALVMRGEGLAAKISDTDPLVEGVAPRQAVALDQASEKAARLVNAYIQKAQEILRSEEP
                     ANMVLLRGFDKLPVLPKYGELFKLCAASIAVNGMYKGVSRLVGMQVLPVDGVTIEDEF
                     STLEMYWEQFDFFYLHIKQTDTHGEDGDFDR"
     assembly_gap    42752..42851
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <42852..43712
                     /locus_tag="P5516_02045"
     CDS             <42852..43712
                     /locus_tag="P5516_02045"
                     /inference="COORDINATES: protein motif:HMM:NF013539.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HRT91252.1"
                     /translation="KEMDFTRLISEQYQTLTKSEKMIANFLRKNQEESAFLSAGEIAR
                     RLDLSEATLVRFARTLGFNSYPAMRSLLQENFRQRITHSARLRGRLDEMRQNGGIFER
                     VTATEIDYLTQALNSIDRDQFNKAVELIKTNEKVYVFGVGPSITLVDLMEIRLRRFGK
                     EVVPLKAYGREVVDFMLAMKPGDLVFVICFFDQNPTLHLVLDYSRKVGATVIMLTDTL
                     ETILGDSADVILSARRGPVGAFHSLVVPMTVINALLLSVASLDQERIMPALDKLDSLR
                     GQLKELNRAG"
     gene            43791..45626
                     /locus_tag="P5516_02050"
     CDS             43791..45626
                     /locus_tag="P5516_02050"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRT91253.1"
                     /translation="MTVTSIEPKDNKEKWLARLRRFFRWLTSPHVVLSIIMVAVMFYL
                     VIIPLYRMVVTTITVADVDLRAIRGAEEGDFTWYHWVRMLTGKIGKLMTYQPAMHSIV
                     VALGATFLALAVGGLMAWLVCRTDIPGRDTIHMLAQVPYIMPSWTIAMAWLVLFKNQK
                     SGGTMGLLEFITGAPTPDWLAYGPIPIIVSSGLHYYTFFFLFIAAALLSIDSSLEEAG
                     ELAGAGRWRIMRKITFPLVIPAIASGFIMTFSKAMGTFGGPNVLGVPVRYYTLSTMLR
                     SNSSIGNYGDAFVTAIVLILFAMVTVMLNQKIVGTRKSYETIGGRGFMSKKTKLRNFK
                     PIIVAIVVIFQFIIAVLPLVLLVYSTFMLRDGKWTLDNFTLAHWIGKGDLSINSGEPG
                     ILLNHKVWLGAWNSVRLSILTALFTALFGVVLGYAIVKGRGTWLSKIVEQMSFIPYVI
                     PGIAFGAVYIAMFTKPVGPIPALYGTFALLVVVSVAKHLPFSARSGVSAMMQVSKELE
                     EAAMLAGASPWRRFARIVFPLTSTGFVSGFLLTFITTMRELSLIILLVTPTTAVLASM
                     TMRYIENGNDQQANAVIILLIVLVLIGNFIISRFRGGSLKKGLGL"
     gene            45639..46724
                     /locus_tag="P5516_02055"
     CDS             45639..46724
                     /locus_tag="P5516_02055"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91254.1"
                     /translation="MDTPIVINNLTKKFGDVIAVNNVSLRIEPHTFLTLLGPSGCGKT
                     TLLRCISGLEDPDEGEIYIGDTLIYSHAKGISVPPGQRELGMVFQNYALWPHMTVYKN
                     MTFALEIQKLPKAEMDKRVKESLEEVKMSGYENRYPREMSGGQQQRIALARMLAYRPK
                     VFLMDEPLSNLDARLRMDMRTELKRLHHISDATTVYVTHDQLEAMTMSTNIAVMKEGV
                     IQQMDTPDMIYHYPANLFVADFIGNPKINLLKGWVSSEDVVDLGKFNIPAYTHRKTGD
                     VVAAFRPEDITIHTEPVEGGVEFSAYSVQPSGADTTIIAHKGDTEIIVKEMGVSKIAM
                     DQKIWLTFNKEAINLYDKATEKLITGS"
     gene            46826..48058
                     /locus_tag="P5516_02060"
     CDS             46826..48058
                     /locus_tag="P5516_02060"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT91255.1"
                     /translation="MKAVIRIATLVLLVSFLAACAKPTAAPVVEPPVVEEPVVTEAPV
                     VEELTAQQQWLKDNQLGEYYTDEQDWAAIEAAAIAEGEVLVYANTSKIEKSAKAFMEK
                     YPGINVQAFDLGGDDVLLKTVEEQKAGAFTGDVWSSSGGAELVGNVLPKGYVWRFIPS
                     TVADRIPEQYTHPLLMARLGTAVFAYNSELNDTCPITNIWELTQPEWKNKFFIEDPIN
                     DASTLSKLLTLVSHSEEMAAAYVELYGTEPVLEEDTPDAGWLWLKKFAQNGPIPQPGG
                     DEVDEAFATPGMKESYMAITSYSNMADVMEGNLVFEPCWGIKPILGIQSQSYLGIINQ
                     APHPNAAKLWIAWQVSEEGRKPWAKFGTYFTDSTYEVPEGQKPLSEMMAVTWFIDEQF
                     AYDHMIEARDFYLINLAE"
     gene            48202..49632
                     /locus_tag="P5516_02065"
     CDS             48202..49632
                     /locus_tag="P5516_02065"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRT91256.1"
                     /translation="MALRTMLFDVGGTLETYSCSRELRIKNTAVFRNFLKENGIILPY
                     SDEQLYEVISSGISAYRKWSIASMIELSTEEIWSQYVFRGHPVDQEKLNRISEQLSCL
                     YELNYYVREMRPEVPAVLAEIKKMGFRIGCISNTQSLTQVPATLEKYGISQYFDPVVL
                     SSKYGRRKPDPAIFYHAARLANSPTGACGYVGDKINRDVLGAKKAGFKLAIQVRHEFD
                     DGEVDEGAEPDAIIENIADLLPLLEASIRENRSISREDERSRIKAVFFDAGDILYYRP
                     QRDKYLNEFLSGHQLNPVPGFEDERKRIKDLAFSGQMRRHDYYRELIQLYGITDEQAI
                     EQGMEALSRDDDTVAIFDGVPETVHTLKERGFILGIITDTAMPITRKLNWFEKHGFGR
                     VWDVVISSKEMGVRKPSPSMYEEALQQTGIRSQEAVFVGHKTHELEGARAVGLKTIAF
                     NYDPDAPADYYIEHFQDLLKVPLLAN"
     gene            49659..>51225
                     /locus_tag="P5516_02070"
     CDS             49659..>51225
                     /locus_tag="P5516_02070"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRT91257.1"
                     /translation="MQRKVKAIFFDIGGTLRVTHPEEGRDMSKIQALMSLIGEKSDPL
                     DFVARVHKGEKAYRRWCKPNYIELSEAELWTRFLLPEYPAPFIRENAITINQFWRESK
                     PKYVLPDMVETMRELSKRGYRLGLISNTTSSVEGYQLLEETGLTRLFSSVILSAEFGR
                     RKPHPSLFIEAARRAGVRADECAYVGDRPSRDLIGARQSAYAEVVIINSAGYSPDEYD
                     PDDYDPEKDTHLTLRPDHWIKRLSELLEIYKPIKTAAQENPQKESEYKLFDVALSTMW
                     HVGQAASFNETFELVRTIGISRFEFNHQVTPQLLATFDCNRNYISTVHDPCPAPRDLR
                     ALKNDDILISSLNEARRGQAVDLVKRTLDLAVSLGSRSMVLHTGSLQCDRARDRRLRQ
                     MYEEGQRGSEEFCQLREEMIAHRAQYAPAHLDAVKRSIAEIITFAKGSGVNIGVENRY
                     RYYDLPLPSEMQEILDLCQESWFGFQLDTGHAHALETLGLCEKGVWLQRFHHRIIGVH
                     LHDVAGLTDHQVPG"
     assembly_gap    51226..51325
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <51326..>52559
                     /locus_tag="P5516_02075"
     CDS             <51326..>52559
                     /locus_tag="P5516_02075"
                     /inference="COORDINATES: protein motif:HMM:TIGR01549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HRT91258.1"
                     /translation="EFLAMVSRGEARYKDWRGLSLQALPVAEKWARFLLAELDYEFVF
                     QRGERLQQLWSASKGSKSIRAYAPLVLHELKNRGYLLGTISHSTPRYLDNSSLADLFK
                     VKVHAPQFGMKKPHPSLFVDAARQCGLSPRQCAYVGDNSWRDVVGPREAGYEMVIFLR
                     NGGGDPGPAAEMMQPDLVISDLREILDYLPQRVGTEPLQPLTELQEVLYDAALSTMWW
                     KKEELSPEAFFTRGRRIGFARFELNHQIPPALLEKIDHQRFSIGSLHDPCPALVPAKT
                     LEQTDVQITSLDENLRQKGVDVLKRTIEQACRMRARLVVVHPGRITGDHSLDDQLRSL
                     FREGKKGSLEYEQLKQSVIANRAERAAPHLQQCLESLREITCFADGSGVMIGLENRFH
                     YYELPVFEEMGKIMDEFTQ"
     assembly_gap    52560..52659
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            52686..53306
                     /locus_tag="P5516_02080"
     CDS             52686..53306
                     /locus_tag="P5516_02080"
                     /inference="COORDINATES: protein motif:HMM:NF013982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF47 family protein"
                     /protein_id="HRT91259.1"
                     /translation="MLKIFKKRRNVFIHLMHEQATLTLQGMDLLKDYLSYPAKQLADE
                     LTMKEKEADECRRILIDELNRTFITPFDREDIFSLSRAIDDIIDYAYSTMTEIVILKV
                     DPTEFMVRICSLLRDAAYELMMAVEQLQDHPAVAAEHAQRAKALENRVETVYREALAD
                     MFQKADDVKGLVRIMKLREVYRHLSNAADRGDEAANVIADIIVKTT"
     gene            53313..54308
                     /locus_tag="P5516_02085"
     CDS             53313..54308
                     /locus_tag="P5516_02085"
                     /inference="COORDINATES: protein motif:HMM:NF013543.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic phosphate transporter"
                     /protein_id="HRT91260.1"
                     /translation="MSFTLILLVGLAVIFNFLNGVHDSANIVATAISSRAMAPRTALI
                     VVAIAEFCGPFIFGVAVANTIGNGVVASDQINSQVIIAALISAIVWNILTWVLGIPSS
                     SMHALIGGLIGAVVAGAGFMAIKLDGVIKVLISLFASPIIGFFAGILIANLIFLLSWK
                     SSPKINGVFKKLQIITSLGLALSHGTNDAQKTMGIITLGLLISGMISSFHVPFWVIFV
                     CALSMAIGISVGGWQLIRTLGSRFYKIRPVHGFASQFASTAVILSASLLGAPVSTTQV
                     VSTAIMGVGAAERVSKVRWGVAINIGFAWLYTIPLTALLAAGIYAFMKFILPMIT"
     gene            54327..>54519
                     /locus_tag="P5516_02090"
     CDS             54327..>54519
                     /locus_tag="P5516_02090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91261.1"
                     /translation="MTDTSTKYDAIFVGNYTKDTIITPEGTRYADGGGMNYAAHAASA
                     LGLKAAVVTRLAKEDEHVVN"
BASE COUNT        12867 a        14367 c        14554 g        12031 t
ORIGIN      
        1 gatatgcagg atacctattt tttggatgtg cccaaccggg gagacaaccc cctggtgctg
       61 cgcacgcaca catcgccagg ccagatccgc gccatgcgcg agattgcatc cctcaaccca
      121 tccaaccctc caccagtacg cgtcatcgtg ccgggaatgg tctttcgcta cgagcaggta
      181 gatgtacggc acgaaaccca gtttgaccag ctggaagggt tggccattgg agagggcatc
      241 acctttagcg acctgaaagg cacgctcaca gatatggcgc gccggctctt tggtccatcc
      301 gcgcgcgccc gcttccgcgc ttcttacttt ccattcaccg agccatcggc tgaatttgat
      361 attcagtgtt tccaatgcca cggaaaaggc tgcggggtgt gcggcggcgg ttggctggaa
      421 atcggcggca gtggaatggt gcacccagtg gtattggcaa acggtggata cgacccggcg
      481 cgctacaccg gtttcgcttt cggtttcggt gtgcaacgta tcgccatgct gcgttatggg
      541 atcaacgaca tccgttattt ctgggaaaac gatttacggt tcttggagca gttttaaggc
      601 ccgtcaacgg tgagcggtga tcgggtcatc acaaacccaa tcccttagaa caactcgcga
      661 ttcacttcac ttggagaaaa cacatgaaag cccctttatc ctggttaaaa gacttcgttg
      721 aaattgacct ttcattggta gaactggctc accggttgac catggcgggt ttggaggtgg
      781 aaagtattca actcattggt ctgccgcttc cgccggcagg cagcaccact gccaaggtca
      841 gcggcctcac ctgggaacca gagaaaattg tcgccgccgc cgtgaccgaa gtgatgcccc
      901 accctaacgc tgaccgcctg gtgctgtgcc gcttggagga tgggcagcag gagcacattg
      961 tgctgaccgg cgcgccgaac ctgttcgaat acaaagggaa aggcccgctg ccacaaccgc
     1021 ttaaggtagc ttacgcgaaa gaaggcgcca tgctttacga tgggcaccag gaaggctatg
     1081 tgctcaccac gctcaaacgc accaagattc gcggggttga atcttattcc atgatttgct
     1141 cagagaagga actggggatt tctgatgaac acgaagggat aatgttcctc gacgacgcag
     1201 cctccgtggg cacgccgctg gtagaactac tgggtgatgc catattggaa gtaaaccttt
     1261 tgcccagttt cgcgcgctgc gccagtatct tcggtttggc acgtgaaatt tccgcaatta
     1321 ccggaaaggc gcttaaaacc ccgcgattca cctctccccc tgacatcggc aaagccactt
     1381 atgcagagct tgaaatcacc gacccggttc tcaatcctcg ttttgtcctg ggtttggtgc
     1441 gcggcgtcaa tatccatccc agcccgctct gggtgcagca gcgccttaaa ctggcgggca
     1501 tgcgccctat caacaatatc gtagacgcca ccaattatgc catgctggaa ttgggggaac
     1561 ctctgcacgc gtttgattac gatgtgctct gcaagcgcgc tgccgggaaa caggtgaaga
     1621 tcattacccg cgccgcaggc aaagacgaaa agatcaccac cctcgatggc gtggagagaa
     1681 cattagacga aggtaccgtt ctggtgtgtg atgaagccgg cattctttcg ctggcagggg
     1741 tgatgggcgg attggaaaca gaagtgaccg ccagcacgcg caacatcctg ctggagggcg
     1801 ccgcctggaa tatgatttcc acccgccgca ccgctaaagc ccaaaatctg ccctcagagg
     1861 cttcttatcg tttcatccgc ggtgtgcatc ccgccatggc agaaaccggt gtgcgccgcg
     1921 ggctggagtt aatggagcac tgggctgacg gcgcagtgga cccgttgatg gtagacgcct
     1981 accccttacc gccaaaaact tccgtggtgg atatcacccc gggcgatgtg aaacgctggc
     2041 tggggcttga cctgagcgcg ccccgcatcg ctaaattgct ttcacgcttg gagttcaagg
     2101 tgcagataga tagcgatctg ctgcacatca cctgccctga acaccgcctg gatatcggta
     2161 gcggtatcac tggcaaagcc gacctgatcg aggaaatcgc ccgggtgcac gggtatgaca
     2221 acatccccga aacccgcatc tccgaccgcc tgcctttgca gctgggcaac tcgcccctca
     2281 agaagaccga cctcacccgt gacctgctgg tagcgctggg gttacaggag gtcattagtt
     2341 accgttggac aacacctgaa aaggaagggc gccgtttgcc caaaggcgca gaagcagata
     2401 acaagcccta cctgcgcatc agcaacccgc tggcctatga aaaggctttt ctgcgccaca
     2461 gcgtgctggc cagcctgctg gacaccgccg aacgcaattc gcgccagcgc gcaaccctgg
     2521 ctttatttga gatcggcccg atcttcctgc actccgaaga cccctccggc ctgcctgatg
     2581 agttgagcag gctggggata ttgctggctg ggcagcgcaa cccgtcaggc tggcagcctg
     2641 cggataacag cacgatggat ttctatgacc tcaaaggtct tcttgaagac ctgatggcag
     2701 gtctgcacat cacggaggca aatttcagcc agggtgaaca cccggggtat caccccggaa
     2761 aatgcgccat catccgtgtg gggtctcaac aggtgggcgt gtgcggagaa atccatcccg
     2821 cggtgcgcga gcgctatcac tggggcgata cctttcaata cccggtcctg gcagccgagc
     2881 tggatatgga cctgctgata gagctcattc ccgagcagcg ccacaccacg gatatcccga
     2941 atttcccagc ggtcgtggaa gacctggctt ttgtgctgga cgaagccact ccggcggctg
     3001 acgtgaccgc gcttatctgg caaaccggcg gaaagatact ttctgagctg caactgtttg
     3061 acgtgttccg cgggggacag ctcgggagtg gaaaaaagag cctggcatac cgcctcacct
     3121 atcaggctgt ggaccgaacg ctgaccgata aagaaaccgc ccaggtgcgc aaccgtatca
     3181 tcaagcgcct agagatggaa ctgggcgcca gattaagaag ttgagatgag cagcctttac
     3241 aacataaagg atggcaaagc tgccatcctt tatgcgttct aataagacca gccgctgatt
     3301 tttgattact cctggtgtgc gcctaaacag gcaaagatgc ggtcgatatc cagctcagtg
     3361 aatctttcat aggcaagtga agcccccagg ttttcatcca gctgctcaag ccgggtggca
     3421 ccagtgatga cagaagaaac ctccttgcgt ctcagcaccc aggcgatggc cagttgcgcc
     3481 ggggtgatat ccaggtcact ggcgatttca gtaaggcatt tgaccacctg gatacgctcc
     3541 ggggtgatgc ggtcgcgaat ccaaccatag tcttccagtg aagcgcggct gccctttggt
     3601 attccgttgt tatactttcc tgaaagcacg ccgtaataaa gcggtgagaa agtggtgagc
     3661 cccaacccaa gctcgcgcgc caccggcatc acctcgtctt ccacgcgttt gcggtgaaag
     3721 aggttgtact gaggttggtc catactgggt gggatgagcc caaactgccg cgcagcgcca
     3781 tacgcctgca taatctgact ggcctcccac tccgaagtgc cccaatacag gatcttgccc
     3841 tggtgtacca ggtcggtcat ggtgcgcacc acttcttcga caggtgtgtc agggtcgtag
     3901 cggtggcaaa aatacaggtc cacatattca gtgcccaggc gcttgaggct ggcatgaatg
     3961 gactcggtga tgtgcttgcg tgaaagcccg cgcccattgg gtcctttcat cgtcggccag
     4021 aacactttgc tcgagatgac cagttcttct cgcggcatgt cagcaatcgc cttgcccatc
     4081 accagttctg cctgaccatt ggcgtacata tcagcgttgt caaaaaagtt gattccctgg
     4141 tcaaatgctt ctttgatcag gctgatggcg gttttctcat cgatctgatt cccaaaggta
     4201 atccacgcac ccagtgatat ttcactgact ttcaaccccg attgaccaag cctacgataa
     4261 tgcatttact tatcctcctt ctgtccagct ccaattccca gcggtttcca cttccgttcg
     4321 tcggcgcgca ggcggtgcat ccctccgccg cgattatgca gcaggtcaaa gttttctggt
     4381 ttaatggcca gctcttcgcc atccagcagc ccgctcacac cagattgccc gggctcgctg
     4441 cgtttattct gacaatactt gcaggttttt gggtcgtgcg ccttgtaatc ctgcggttct
     4501 tcataggtgg tgatgtagcc ttcgtagttg cgcaaaatga ccttgtgatc ggaagaactg
     4561 aggtgataat tgggcatcac cgggatcttg ccaccgccgc caggcgcatc taccacgaag
     4621 gtgggcaccg cgaacccaga ggtatggcct ctcaatcctt caatgatctc gatgcctttc
     4681 gccaccggcg tgcggaagtg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4801 tgcgcgagaa agtctggtta gggtcgccca caatgcgcgc gcgtgtgcaa tagcggcaat
     4861 aagaggcaca ctgcgtggtg accagcatta atacacggtc agggtagcgg tgtaccagcc
     4921 cgggcaccgg agagtggcgg tcttcagcca acgagtcttc catcatgccg gtaaaggcgt
     4981 ttatttcgcc ggcggtagga atgatctgtc ggcggatggg gtcatgcggg tcatcaggat
     5041 cgatcaggga tacatagtag ggggtcacat ccacgcggaa aaggccgtcg gtgctgagcg
     5101 cctggcgttc gctttcagtg agggtgaaga ttttatcgaa atcctcaact gcgtttaaac
     5161 gccggctcaa ttgccagcgc cagtcgttcc attgttcatc aggtacatct ttgaaaaagg
     5221 gagcccgttt agagagaaag ggagtgggtg tcatacatcc tccataacct gggttgagct
     5281 gaaatttcct catttcattt taagggctaa tggcaggatg gtcaacatat tgatttaacc
     5341 ggctgccatc caagcgggtg acagccgcag cggctgattt gagaagcggt taatccacct
     5401 ggtaaggcac ttcagtgatc acgatcttct tgctatgcag caacgcttta cgcaaggttt
     5461 ctgcggcgcg catatgcaga aaatttaccc accagtgacg cggaacaaat tggggcacaa
     5521 ccagggtaat gatctcattc ggcgccagaa tcagcgatat tttttcaata tacgccagca
     5581 agggttcaag aaacaaccgg tagggtgagt caaggatcac caggcggtat ccctcgcccc
     5641 agcgctccca tttgctcttc actttcgctg tttccgcggg gtcaatagaa acgtgcacca
     5701 cggtaacatc atcagataaa gatttggcaa attgcagccc cgcgagtgtg ccgttatgca
     5761 ccccgctgat aggcataatc acgcggttgc gggtgatcat gcggctcgtg tgtgggtttt
     5821 ccaatgaaag ctggcgggcc atttttttat aatgacgatg aatctggaaa aaaatggtca
     5881 ctaaaagcgg aacgaggatg atgattatcc aggcgccatc tatgaacttg gttaccgcga
     5941 atatgattgc aacaatggaa gtgcaaaaag caccaaaccc attgatcacc attttatgca
     6001 cccaaccctt gtcatagcgt agcgtagacc ctgattccac aacctcatcg cctggcttca
     6061 ggtggcccgc cttccaccaa cgccgcgcca ttcctgtttg agacaaggtg aaggaaagga
     6121 aaaccccgat agcgtagagc gggatcaggc ctgtcactct cgctctgaag gcaatgacca
     6181 ataatgaagc tgtaaccgcc agcagcacaa taccactgga atacaccaaa cggcttccgc
     6241 ggaaggtgag ctggcgcggt aggaatccat cagcagcctg taacgcgcca agccgcggaa
     6301 aatcggcaaa tgccgtattc gctgccatga tcaagataat cgttgttgag ctgatgacaa
     6361 gcaaataaag gattccccgg ctgccataaa tagttctcgc cagttgcgag actattgttt
     6421 cgctatcaga aggaatcgtc ccaacctttg tgctcaaaaa cgtgatgctc agcagtaaca
     6481 ctccaagtat tcctgacatt aaaagtaacg tttttcccgc gttcttcgct cttggttctt
     6541 taaatgccgt gattccgttc gtaattgcct caacgcccgt gagtgaggtg gtgccattgg
     6601 caaaagcctt caagaggaga aaaatggtta caggctgcaa gtattcgtgt tccagcagcg
     6661 gtggctcaac gactattccc agggtgccgg aaaaataacg cgcaaaaccg gcaacgaccg
     6721 tgatcaacat caacaccagg aaaaagtaag taggaatggc aaaggtgata ccggactcct
     6781 tcaccccgcg caggttaatg atggtgacaa aaaagatcaa acccaccgca atcagcacgc
     6841 gaaaagagtt cagacccggg taggcagaaa caagttgggc tacccctgat gatatcgata
     6901 ctgctacggt gagaatataa tctgtcaaca aagcagcgcc tgcgacctga gccgggagtt
     6961 cacccaggtt atcgcgagca acaatgtatg cacctccccc gtttgtgtag gcatgaattg
     7021 tctgttcata tgagaacgtg agtatgatga gaagaacagc aatccccacc accaacggca
     7081 gagcaaggtg cagcgctcct gctcccgcca cagccaggat cagcattaat tcctgaggac
     7141 cataagccac agaagacatc gcgtcagaag aaaagaccgc caacccaacc agtttcccga
     7201 tcgtttgatg aggggcgtca gccgtcgcca gaggacggcc tataagccag gtttgaagtt
     7261 tcttctcacg cggtatgccc gaggattgtt caaagatatt gataggttct tccattggat
     7321 tcatttcttt attgttccca attcctgctt taaaaaaaat agactcacca tttcagaggt
     7381 aagtctacat atcaggcgta aggattatct aagagtcttg ggcaataaat aaagaaaaaa
     7441 taaaggcttt actcccctgt tttaaacctg tagcccacac cagggtcagt aaccaacacc
     7501 actggagcgt caggatccac ttcgatcttt tttcgcagct gaccaatata gacccgcagg
     7561 tattcaaggc ggtctgcctc ttcgaacccc catacatggg tcagaatagc ctgatgggtc
     7621 aagacacggt cagcgtgcga agccatgtat gcgagtaact taaattcagt ggctgtgagc
     7681 ttgagttcag cgccatccag gctgaccagg tgacgcgcca ggtcaatcgt cagtctgcca
     7741 gccctgatga ccgtctctct attaccgatg gactgggacg agtggcgtag cgcaactcga
     7801 atgcgcgcca gcagttcctc gatgttgaaa ggtttggtga ggtaatcatc agcgccttta
     7861 tccagagcgg caaccttgtc acgctcacca tcgcgcaccg aaagcacgat gatgggtatg
     7921 gttgaccatt cgcgcacctg ttcgcaaacg gagatcccat ccatgtccgg cagcgtcaga
     7981 tccaggataa tgacatcagg tagttgagag gctgccagcg cgatgccctc ctcgccggtc
     8041 acagccgaaa tgacgcgaaa atgcccagag ctgaggatgg tcttcaatgc ccgcagtatt
     8101 tgcggttcat catcaatcaa caaaattaag gggtccttat ccatcgatta catcctttgg
     8161 taatcgcggt aaagcgccat ccagggtgag aggaagggta atattgaagg taaacccatc
     8221 tgctgtgttt tccgcccata ttttaccttc gtgcgcttcg acaatgcctt tgcagatggg
     8281 caaccccaaa ccggtgcctg taaccttatc ggtctcggaa attcgaaaaa atttttcaaa
     8341 gatgtgttcc aggtgctctt cgggcaccgg tgggctctga ttgatcactt taaccaacat
     8401 ctgtccagca gccacaccaa ttttaacaag gatgggagta ttctctgggg cgtacttgtt
     8461 actgttactg aaaaggttgg tgaaaacttg gcccatcatt acataatccg tcggcaccag
     8521 gggtagctca ttctctacta aatatttaat ccggtgttct ccgatttgcc ttcgcatttt
     8581 ttcctcaacg cctttgatta tttcaataag cgaattccag catttggtcg gtttcaacgt
     8641 accggcttcg atacgcgaca tatctaataa attgccgaca agcaaattca acgcgtctgt
     8701 ttcttcatcg atggtggcca ataattccgc cctggctgaa gtctcccatg gaaccgcgcc
     8761 tccccgcagg ctggtaacgg aggctttaat cgccgccaga ggagagcgca actcgtgaga
     8821 gaccgaattt aacaaggcgg tcttcacccg gtcgctttct tccaacactt tagccaggtt
     8881 ttcgcttcga aaaaggcgaa ccctttcaat agccagggcg ccctggttgc agaacgctga
     8941 aagaagccgc gtttgttcta cagtgagttt attttgatcg aaccacagat tcagtgttcc
     9001 ttcgtcattt ctggcggtgc tcatatggat gaccaggtca gccggggttt cgagcctggc
     9061 ggtttcaggg tattgataaa tgatcagctc attggatgac aggtcccagg tggaaatttc
     9121 aatctggtca aaatgaaatg tatcaaaaag tcgggtggcg agcagtcgga tggtctctcg
     9181 aatatccgtc accccagcta aagaagannn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9301 nnnnnnncat attaatcttt tattagaacg ccccaggctt ccttgactca acttttccgg
     9361 catgttatca ttgaagccag tgaattagca ctcaggcttg tcgagtgcta aaaagatgaa
     9421 aaatgactga actgacagac cgccaacgca tgctgctttc tctggtaatt cacgagtacg
     9481 tccgctcggc gaacccggtg ggctctaaac acgtcgtgga acagttcaac ctggatatga
     9541 gctccgccac agtgcgcaac gagctttcca cactcaccga actgggttac ctgcgccaac
     9601 ctcacttatc tgccggcagg gtgcccaccg aagatggcta ccgcctgttt gtgggcaata
     9661 ttgtgcagga aaccgagtta ccagattcta cacgccgcac catcgcgcat cagttctatc
     9721 agatgcgcca ggatgtagaa ggctggactc gtctggcggc ttcaatactg gcgcatcaat
     9781 cacgcgcagc ctctcttgtt accgctcctc accccgagca atcgcgcttc aagcatctcg
     9841 aactgatcgc cgcgcgcggt cgccaggtat tgatggtgct ggtgctgatg ggcggtgaaa
     9901 ttatgcagcg ctttattacc cttcccgagc cagtctcgca ggaacagctt tcaagcgcgg
     9961 ctgatcggtt gaaccttatc ttcagcggtc agaatattgg agagatgtcc agtgactcca
    10021 gtgagctcgt tgcactggag caggagttct accagattgt gctggatgaa atggaacagg
    10081 cagacgcgat gatttctggc gaggtggtgc tggatgggtt taccaatgtg ttggcagaac
    10141 cagaatttgc cgactcagag gaagcccggc gcgcgctgcg cgtgctggaa gaaaaaacgc
    10201 tcttgaacga cattctgtcc aaaacggtgc tgcatggctc ttcgctgggc ggggtgcagg
    10261 tgctgattgg cggcgaaggg atttatgacg ctctttcgca atgttccgtt gtcctcgccc
    10321 gttacggcac ccctgggctg gcaaccggca ccctgggtgt gctcggcccc atgcgcatgc
    10381 cctacggaag gaccatctcc attgtcaggt tcctttcagg gttgctttca gacctggtga
    10441 acgacacgat tattgaataa ccgcgccacg gaatttagaa tagaggaata gatcatggac
    10501 gaagcaaaaa aacacagcgg taaaacccat cagccagcaa cagataaaag cgaaaaatac
    10561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn aaaccgaatg ggggtcatgg
    10681 cgaagcctgg gcgaccggca tcgaattgat cgcccgcaag ctgctttcca tcatcgaagc
    10741 cgagggtgta gagcgcatct ggcagaaccg ggtccccttt gaccccacca ttcacgaagc
    10801 catttcaagc gaggaaaacc ccgattttga aagcggcgat gtgatcgaag tcacgcgaca
    10861 aggctataaa ctgggcgacc gtgttttacg acccgcaatg gtacgcgtcg ctaaataaaa
    10921 tctacattgg agagaaatta tgggaaaaat cattggcatt gatctcggca ctacgaactc
    10981 agtggtagcg gtgatgagcg gcggtgaacc cgtcgtcatt ccttctgccg agggggagcg
    11041 tcttattcca tccgttgtgg ctgttaacaa aaaccacgag cgcatcgtcg gtcgcgctgc
    11101 ccgcaaccag gcaatcgtca accccgaaaa cacgatcttc tcgatcaaac gcctgatggg
    11161 acgcaaattc agcgaccctg aagtgcaacg caccatcagc cgcgtgcctt acaaaatttc
    11221 cgcggccagc aatggcgaca tccgtgtgac cctcgacggc aaagagtaca gcccccccga
    11281 gatcagcgcc atgatcctga gcaagctcaa agcagacgcc gaagcctacc tgggtgaaac
    11341 ggtgaaccag gcagtgatca ccgtaccggc ctatttcaat gacgcgcaac gcaacgccac
    11401 caaagacgct gggcgtatcg ccgggcttga ggtactgcgc atcatcaatg agcccaccgc
    11461 gtcttcactg gcttacgggt tggataagaa gaaaaacgag gtgatcgcgg tttacgacct
    11521 cggtggcggc acctttgata tttccgtgct ggatgtcggc gaaggtgttt tccaggtgcg
    11581 ttctaccaac ggcgatacct tcctgggcgg agatgatttt gacctgcgtg tgatggatta
    11641 ccttattgaa gaattcaaaa agtccagtgg ggtagacgtt cataacgacc gccaggcgct
    11701 gcagcggtta aaagaagccg ccgaaaaagc caagatcgag ctctccacgg tgcagcagac
    11761 cgagatcaac ctgccttacc tgaccgcgga cgccaacgga cccaagcacc tggtgctcac
    11821 cctcacccgc gccaagctcg aacagctcac tggagacctg atccagaaaa ccatggagcc
    11881 catccgccgc gctctctcag acgccagcct taaagccaat gaggtcaacg aggtggtgct
    11941 ggttggtggc atgatccgca tgcccgcggt gcaggacgct gtgaaacgcg agttcggcag
    12001 ggagccgcac aagggtgtga atcctgatga agttgtggct ataggcgcag ccatccaggc
    12061 gggtgttttg ggcggcgatg tcaaagacat cttgctgctc gatgttaccc cgctgaccct
    12121 ttcagtggaa acactgggcg gcgtatccac cccgctcatc gagcgcaaca ccaccgttcc
    12181 cacgcggaaa agccaggtgt tttccactgc gtctgatagc cagacccagg tggagatcca
    12241 cgtgctgcaa ggagagcgac ccatggccgc ggacaacaag tcgttgggca agttcaacct
    12301 cgatggcatc cctcctgcgc cacgcggcgt cccgcagatc gaagttacct tcgatatcga
    12361 tgccaacggc atcctcaacg ttaccgcgct ggataaagcg accaaccggt cccagcacat
    12421 caccattacc gcttccagcg gtctcacaga aagcgaaatc cagcaaatga agcgtgacgc
    12481 ggaagccaac gcggaggctg accgcaaaca aaaagagctg gtagaagtgc gtaacaacgc
    12541 ggataacacc atttacgccg cggaaaaatt gctccgtgaa aacagcgaga agattcccgc
    12601 tgatacgaaa aaggaagtcg aagaggccat tgccagcctg cgcggcttga tgaacaatga
    12661 ggatgcaggc gccattcgca ccgccaccga agcactggga cagtcgatcc agaagattgg
    12721 cgccagcatg taccagcagc agcctgaagc cggtgggcag ggcgcgccag gtgaacagca
    12781 ggggcagccc ggaactgaca gcaacggcgg caaccctcca gatggagatg ctggcgacgt
    12841 ggtggatggc gaatttaagt cggtttagac caggcgactg ccatccgtta taatctgacg
    12901 gggcgcatgg agaaattttc tccgcgcccc aggttcacct taaaccgccc acaggaataa
    12961 tgatgtatga ccaagcgtga ttattatgaa gtactgggtg tcaaccgtaa cgcttccgcg
    13021 gaggagctta aatccgcgtt ccgcaccctg gcgcgcaagt accacccgga catgaacaaa
    13081 gaagccgact cagaagagaa attcaaggaa atcaacgagg cttacgccgt cctttcagac
    13141 cccgataagc gccgcgctta tgaccagttc ggtttcgctg gcgtgaatgg cgcgggcggc
    13201 gtgcctgatt ggacgaccat ggatttcgct gatatttttg gcgaattttt cggcttcgcc
    13261 accggtgcat cacgcgcgcg tcagaacgcc ccgcggcgcg gtcaggacct tgcttatact
    13321 gtcaccctga cctttgaaga agccattttc ggggttgaaa aagaggtcga gattacccgc
    13381 gatgaagtgt gccacacctg cggcggtaag ggggctgaac ccggcaccaa cccggcgaaa
    13441 tgcagcaatt gcgcggggcg aggagaagtg cgccaggtgc ggcagacctt tttggggtcc
    13501 atggtgcagg taaccacctg ccctgtgtgc gccgggcgtg gagagatcat cggcacgccc
    13561 tgtcacacct gcaacggccg ggggctggaa cgcaagacgc tcagcaaaaa ggttgccatt
    13621 cccgggggcg tggataacgg tacacagatc cggctggctg gtgaaggaca gcccggtatc
    13681 aacggtggac ccactggcaa cctctatatt gaaatcaagg tcaaaagcca caagttcttc
    13741 cgccgtaaag agaacgacat cctgctcgac ctgaacatta atatcgccca ggctgcgctc
    13801 ggcgcggatg tcgaaatccc cactgtggat ggtccaacca agctggtaat tcccgctggc
    13861 acgcagccag gcaaagtgtt cactctgcgc ggcaaaggca gccccatctt gcgcagcaac
    13921 gcccgcggtg atcaaaaagt gatcgtcaac gttgaaattc ccacacgcct gacggacgaa
    13981 caacgccagc acctccaggc gttggcagcc agtatgggca cggaagtaaa accgcaggaa
    14041 aagggttttc tggacgcgtt gaaggaggtt tttggtggct gagggtaaat ggctgcaggt
    14101 ttcgctcacc gtggatggtg aactggctga agccgtggca gaagtgttaa accgttttac
    14161 cagcggcgga gtggtggtgg aatctgacgt gatttaccgc aacgctgaag atgaaggcac
    14221 cccttacggc gcggtcagag tgttcggtta tctcctcata gacgagaata tcgaggtaaa
    14281 ccgtcacaag ctggaagaag cgttgtggca cctgcgcgcc atacgtgaac ttccgcagcc
    14341 cgaataccgt gaaatcgccg atgaaaactg gatggaagcc tggaaagcgc attatcaccc
    14401 tattcccatt gggaaaaaac tgctgatttt accagcatgg atcaagcaga aagaccaaag
    14461 ccggctggct gtgaagattg acccgagcat ggcttttggc accggcacac atccctccac
    14521 tcagctgtgc ctggagctgc ttgaagacta cacagcgccc ggaaaaccgg tcatcgatat
    14581 cggctgcggg tctggcatcc tctccatcgc ggcgctcaaa ctcggggcgt ctaaggccat
    14641 cggcgtggat atcgacgcgc cagccatcaa agccacacac gagaacagcc gcgccaacgg
    14701 cgtggaagaa aaagtagaaa ccggactggg gtctgttgag gaagtaaaaa ggggggattt
    14761 ttccgcgcgt caggcgccgc tggtgctggc gaacatcctc gcgcctgtgc tcatccacct
    14821 gtttgaagtc ggtatggcac agctggtgtc gcctggcggt gtcatcctgc tggccggcat
    14881 cctgtctgaa cagggcgcca gcgtgcgcgc cgcgggtgaa gcgcgcggtc tcacttttgt
    14941 tgaagagcgc tgttgcggcg attggtcagc gctggtcatg cgccagcccc aatagaagaa
    15001 gatttgacaa agcgctttgc ggacaggctc gcggtttcct gttaattgca gacggcacta
    15061 aaagcagaac cagccctgcc cccgcggact ggggttaatc atcaaccaga gtactcgctg
    15121 cggtatggcg ccttggcgtc aagatcgccc acaaactccg ccaggttacg atgccccaat
    15181 atctgacgga tagactggat ttcgccaatg tggtaccagt aatggtagat gacccgcagc
    15241 atggctgagc ccaggttctg ccctaccggc ttaccgagat gcaacaggtc agcctgtaaa
    15301 tcagccgtgg tgagcttttc cagttctgcc gtggtagcgc gcgttaccgc gtgccaggcg
    15361 ttcagtacat cttccaatgg cggcgaactt tttggcgcgc cgtgagcgaa acgattcaca
    15421 aagtcaggat aatgggtttt accgtgcaac aggtcatgcc agattttctg ctcatgccag
    15481 gtcatatgtc cgatgatcca gctgatgcag ttcatcgggc ccaggtgttt caccgcgtcc
    15541 tcagcggaaa ctccttcaag accgcgctcg aactcactgc gggtgaagaa caattgtgcg
    15601 accagtggat gtatcatctg caacctcctt gctcacagcc ctgctgaacc ttttctttaa
    15661 tcatacaact ttcaacgtac gaacgaaaac aaccctcagc ataaaaaatg gcggagaaac
    15721 ctggttctct cgcaaagccc tattcaagga taatcaacga agtggtgatc tcggctttgc
    15781 ctttaagcgt agccgccacc gagcagtact tcccttctga aagttcgatg gcctgcgctg
    15841 catctttttc ggtgagcgcg ctgcccttca tgcggtaagt gaggtgtatc ttgcgaaagg
    15901 tccagggagg atcttgctcc tgctccgctt cggcgcttat ttccaggctg gtgagcccgg
    15961 cgcgcttttt gttaaggatc tccaccacat ccaccgcgct gcaggaagcc agcgccacca
    16021 gcatcaactc ggaaggcttc attcccaccc cttcatcggg cgtggaaagc accaccgtgt
    16081 gcctggtgga gtccatgccc acgaactgtt taccgccaat ccatttgact gaagctgaac
    16141 tcatgctgtc ctcctgttgc tcatgtcttg cggattaccg ccagcatttc gacggccagt
    16201 tttcggtgtt gctcaaggct gaggtcatcc gcgagcaggc agtcattgac gatttgcccg
    16261 gtatagacat caagggcggc ttgcaaagca gagcgggcgg cgacaaactg ctgcaggatg
    16321 tcgctgcaat cgcgttcttg cgcgagcatg cggttgatgc cgcggatttg tccctccaga
    16381 cggttgaggc ggttgatcac ctgctttttg ccattctctg atgttacttt catcggtatc
    16441 ctgtcacgca gggaggttta ccccgctcag gtgaacccgc cagagcctga gccacagctg
    16501 ccggttgata ctgcccctcc cgaagcgttg ccggcggatg agaacaacga aagctttttt
    16561 cgcgtatctt tgccggcgca gcgtgggcag ggagggcgct cgccagcctg gtcaaagcgc
    16621 atcctctttt caaattcatg tccacaatca gcgcaaatgt actcgtataa aggcatgcat
    16681 cgctcctgaa acaatatact ccaggggagt attttatcac gcttcgccac cagtgcaagg
    16741 gccgcctggc taactgtaaa aggaagtttc cctcttgaca ttgaacatat gttcgtgtat
    16801 gataatagta caaatgaccg ataaatcgag caggaggcag ccaatggcac gcagacaaac
    16861 cggcttaagt gagaggcacc gcaagatcat ggagtttctg accaggtttc aggattctca
    16921 tggttattcc ccctccatcc gccagatcgg ggacagcatt aacgtcaaat caacctcgct
    16981 ggtggattac tacctcaacc aactgcaaaa cctgggctac attgagcgag aagaccgcat
    17041 ttcgcgcagc atccgcgtgc tgcagccaat tcaacccggg gtctccctgc ccgcccgggt
    17101 aggagaagct ttccgcggcg ccgccagcgc ccttgccagc atggtgagca tccctatcgc
    17161 cgggcgcatc gtggccagcg aacctatccc tatgcctgtt tcagacctca actattatga
    17221 cgctgaatcc agcgtggata tcgcgcgcag tctgctgccg gcgaaagatg tcaacgatct
    17281 cttcgccctc gaggtgagcg gagattccat gatcgatgcc atgatcaacg acggcgatat
    17341 cgtcgtgatg aaaaaggcct cctcagccaa taatggcgag atggtagcga tctggctgga
    17401 tgacaataac gaaaccaccc tgaagtattt ttacaaagaa gcagaccgta tccgcctgca
    17461 gcccgccaat cccaacatgg agcccatttt cgtaaagaac cccaagagcc tgcgcatcat
    17521 gggcaaggtg gtaatggtca tccgccaggt gcattcggta gcgggataag ccaatcagat
    17581 acgcctgctg ctgccctgtt taaattaact gcgtccaacc tgcgttttta tagatgtgga
    17641 gcgctttccg ctctacccat cattagcagc cggtaggctg ttggcccaac cccgttatcc
    17701 tttcttatca accctcgccc tttcaggcct ggggggtgat gaatcaggta ttgacatcag
    17761 cagcgggctc atcgcccggg catagggttt gtagcaggcc tgcgcatcca cgttggtacc
    17821 cgtgccagca ggctatggaa cgaccaggat ttccacccac aaccaaccta ccgggtaacc
    17881 atccggcatt tgcaactgcc agtagccaat ataattgccg ggcgtatttg gcgcgaccat
    17941 atccaccgac atttcgattt ctttttttga ataaacggtc gatcccaggn nnnnnnnnnn
    18001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    18061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnng gggtaaccgt acccagggtg atgtcgtgtg
    18121 tcggggtggg ggtaatggtg ggcgggcgca tggcctctac cgtggctttc agaaaatcag
    18181 tgccgattgg tacaaccacc ggagtcgagc cggtctttcc agccagtgaa cgggagcagc
    18241 ttagggtaag aagagccaga ataaccagca gcgggatgat ttttcgggac attttattta
    18301 ctccgatggc gcgaggatgg cttgagaaac ttgccaataa aacgtgtcaa cagtttaagt
    18361 ataccggttt tcaattaccc tgtgggtctt gggctgaaag gttacacaaa gagaggacaa
    18421 tgcgggctta ttcttctgaa tatccgcaaa gcgccaaagc gcctggcccg gtactgatac
    18481 ccagcacagg gccggtgatg ttcaggatca attctacagg gttgaactcg gggcggatgc
    18541 gttctgccag cgcctcagcc tctgccagtg aatttccgtg caacaccgca atatgcaggc
    18601 ggttgtggct tcgcagctgg gcgaaaaatt tctgatacaa catttctacc agctgcttgt
    18661 gagtcctcgc catggcggcg ggttccacca agccggaatg atgattgatc gaaaccagcg
    18721 gttggatctt gagcaggctg cccacccatt tcaccgcgtt gccgatgcgg ccacttttct
    18781 gcaggtactc aagtgaatcc atggcgacat actgcactaa ctttttcttt aagtcatcca
    18841 gcatggtcag tatctttttc acttccatgc ccgcgtcgcg agcgcgtgca gcagccagca
    18901 cttgccagcc gagactcatg gtcggtcctt ttccatccac taccgacacg ggaatcttca
    18961 ccaacctggc tgcttcgcac gccatatggt atgcgccgct catcgcgctg ctgacggtga
    19021 gggtgataat ttctgtagcc cctttagcgg ccacttcttc atacgttttt tgaaaatccg
    19081 ggattcccgc ctgggatgaa gaagggtaga cagggtctac ttccaggcgt ttgtaaaact
    19141 ccaacggagt catatccacc cggtcgcgga actgctcatc tccccagata atgtagtgag
    19201 ggataatggt tattccatac tgctcgatga gagcgtctgg gatgtcacag gtgctgtcag
    19261 tgataatgcc gatcatttca ttccctcgcg attgtgaatt gattaatcat acccccaaaa
    19321 gggcagccac gcacgtttct aaacatcgag agtgtagaaa cttggatcat tcaccatacg
    19381 gccttttccc tttttccaac tccttcaggg tatttattct ttttgcacag tcattaaagc
    19441 gcgtgagcaa ccacagggca tagatagcta ccccgccaat gataaccaaa aaaccggcaa
    19501 ccatatagac gctggtatcc ggcatcattc accttcttcc tggagcgcgg cgcgcctctc
    19561 gttgatttcc gcctgaaggt tgcccaggcg gtagcggtgc cagtacaggg caatgaaaag
    19621 gatggaaaag gctgccaggc tgctaaagaa ggcagtctgc atgctggggt tcatggcgaa
    19681 aagcccgttg gcagaagaat ctgccgagcc gatcattacc gggtgaatgg tccgaaaaac
    19741 gcggatggaa aagaacgtaa gcggtacgct gatgaagcca acaaccgcgt acactgcagc
    19801 tagttttgcc cgccgccgcg catcatccaa gccactgcgc aacagcaggt aggcggcgta
    19861 gatcaattcc atgatgagcg cggtggtcag gcgcgggtcc caggtccacc aggtattcca
    19921 caccgggcgt gcccaaatgg agccgctgac gatgcatagc gccatcaaca ccatgccaat
    19981 ttcaaccccc gccaaccccg cgacatccca tttcaggctg cctgtcttca gacaggcaac
    20041 agcagcgata gttgccacaa agaagcccag catccccgcc aaaccagcag ccacatgaaa
    20101 gtagaaaacc ttctgcacgc tgcccatggt caattcaaaa ggcgcgtaga atatcaccag
    20161 ggcgaaagcg gtagccaaag ccagcagggc gagaaagtca agaaaaatta aagcgcgagg
    20221 agtcttttcg ggtttcattg ctagccaggt ctattcttcg ataattctat caaacaccag
    20281 caaacccaca gcagcaaaaa gcagatcgta acccaccagc aaaaccagcc aggtatccag
    20341 ttcggctggg ttaccaccct gcaagagaat cgtggaagcg cgcacacaag ccaacaacaa
    20401 ggggatgatc acagggaaca gcaataccgg cagcagcatg tcacgcgcgc gcaattgcag
    20461 ggtcaacgtg gagagcaacg tgcctgtgag gatgtacccc agggttccca gcaagatcac
    20521 tcccaacccg ccccaagaga aaagggagag gttattcaac aatgcataca ccggcacgat
    20581 gatggcagcg gtgatgagca ggtaaaccca gttgaccagc gctttgccaa aaaaaatggc
    20641 tgtgcggtcc accggcgcca acagcagagc gttgatcgct tctcggtctc tttccaatgt
    20701 caaagagcga ttaaggctga tggtgccggc aaagcacaaa gtcacccaga tgactccggc
    20761 tgctgccttg cggcgtatct ccacagagag gtcgaaagcg aacacaaaca ccagtatgac
    20821 ggtcagagcg aaaaccagca tggtgccgag tatctcgcgc gtgcgccgct ccgcgagcaa
    20881 gtccttccag aatatcgcca ggatggcgcc gatgaagggc ttcacgcgtt caccccgtgc
    20941 cctttgtggc tgccagaaaa gacggtgagt tcattaaaag ttgccctgag cgatttcccg
    21001 ctgagtcccg cgctggggct cgaccaggag atttttccgt catgcaacag gtcgacgcgc
    21061 gtgctgatgg cgactgcctg attaaaatca tgggtcgtca gcagcaccat cttcccgtcg
    21121 gcggctgcct ggcgaatgag catttcacac aactcggatg aacgctggtc aagcccggta
    21181 tagggttcat ctaataacag caggtcgggg tcatgcagca gggcgcgggc gatcgccagt
    21241 cgctgctgca ttccgcgtga gaaggtgcgc accagttcat ccgcacggct gttcaaagcg
    21301 accatttcca gcacctggcg aattcgtttg tcgctatcag caatctgata gagacggctg
    21361 aaaaagcgca gattttcgcg cgcactgagg ttgtcataaa gcatggaggt atgcattacc
    21421 acgccgatgt ggcgacggta ttcagcgggc gcgttgagag gtgtgtatga gccgaagcgc
    21481 accatgccct gctccggccg caagaggttg gccagcacgc gcagcagggt ggttttacca
    21541 gcgccgttga tcccgaccag tgaaacaatt tcggcgggtt caaccgtgag tgacagcccg
    21601 cgtaaaactt ggcggttgcc gaaagacctg gtgaggttga ctgcgttgag catcgcgcgg
    21661 cgcgtttctt caggcagagc ctgggggaga atcaaccatt ttcaactcgc gcaaggcaac
    21721 agccgcttcc tgcagcagca aggttcgctg ttcctcgtgc tcctgctggc tcattttacc
    21781 cagcccgaaa tcgaaatcca gctcgcggat gcgctcgagg atggcctggt aagccgcctg
    21841 tttgagcgct ttcacatcag gcgcgtaggt gctgccgcct aatggttgat gggtgaagag
    21901 cgggcgggtg atgtaaaaaa acaccagcgc gaggaggata accgtgataa caattggtat
    21961 gagcatcatc gcattttctg tgaactattt tgccagttcc agcatggcgc ggatttcatc
    22021 agcggactga aagggaccaa tctgaaccgc agccagcctg ccatctttgt caacgaggta
    22081 agtctcaggt acagcctgca ccttgaaaag acgggatatt cgcgacccca tgtctggccc
    22141 gtttggaaac gtaatgccat atttttcgag aaaggcctta gcgtcatttt ccgtatccac
    22201 ataattcacg cccaggaaaa tgatatcatc tgaatccttc acttcccgcc agacttcttc
    22261 caggatggcg ccttcttcct cacaggaagc gcaccatgaa gaccaaaagt tgagcaggat
    22321 gacgcgccca tccatcctgg atttttgata atgttcgcct gagaacgtcg tgatactgaa
    22381 atcagggatc tctgcgccga cctcaaaacc ggtctgtcgt gactgtttca accacaaccc
    22441 cagcgttata agcaatccaa ccagtaacaa cccaaagatc accatccagt acggaaagcg
    22501 ttttttcgct ttgttcacag gatcctgcgt gaaaaccggg agtgttgtca ctcaacacct
    22561 tccaggtcat tttcaatctg gctgatgaag tcctctgcag ccattctaca cggtccttca
    22621 tttgatacct cttgagccgc tggaattgtc ttcggcttgc ggatgacccg gatggcgatc
    22681 agaatgcccg cgaccagggt gaggggtgga accaggtaaa tgagccagtt gaatccgcgc
    22741 cgcggcggaa ctgccaacac ctggtctcca tactgagtgg caaaataggc tttgatttct
    22801 tcatcattcc agccaagcgc gagcttctcg cggatcagtt cgcgccactg cgcgcaggct
    22861 ttggtgggac acacctccag cggcacgttt tcgcacaccg ggcaatagag ctcagaagcc
    22921 aggcggttga cctcatcatc actcaccggc ggttcagttt gcgcgtatcc cggcgtttgt
    22981 acgatcaaca ggaaaaacag ccatatcacc agaagaaccc gtttcatttt tcctcctggt
    23041 gggtacgagg gatatttttt cgcctggtcg tcggccacaa cgccaccaac gtgccgaaaa
    23101 ccagcacacc ggagccaatc cacaaccagt tgatcaacgg gttgcggtaa atcttgaagg
    23161 tcgctccttc tgatgaaatt ggctgccagt caaccaggat gacatacagg tcatctacca
    23221 gggtgctgcg aactcctggc agggtgacag attgctgtga tacgtagtag gtatcgctgc
    23281 ccgggaaaac ccgtttcaat tcccggttgt ctcttttaat catcaccgag gctcgcgcgc
    23341 tgttacgtcc atctgcatta tcggtcacct ccagcttctc gtaggtgaaa gtgtagccgg
    23401 aaagagacag cgtttcgcct gctgcgagca ttccctgggt ggtcgtctgg aaaaactcga
    23461 tgccaataat gccgagcgcc atcaaggcaa cgccgatatg aatgaggtat ccaccgtagc
    23521 gccttgagaa acgtctgact agggtgccaa atgcctgcct cacaggttcc tggcggttag
    23581 atgccctcag ggtcaccgtc cggtacgtat ccagtaccgt ggtgaaaatg gccaaggtga
    23641 ccagccacac cgccagcagt gcgaaccagt tacgtacacc cgcgagaaaa acgataagcg
    23701 ggaccgccag cgcagccaaa gcgggtttcc acaggtgccg ctccaggttt ttgctggttg
    23761 agacacccca catggtaagc ggaacgatgg ccatcaatag caataacaat ccaaacagtg
    23821 gggcagtggc gctttcataa aaaggcggcc caactgtgac cttctgacct gtaagcagtt
    23881 ctgagaagat agggaaaagc acgccggtaa ggcagataag aaggagcgcg gtgaacagca
    23941 ggttattgaa aagaaaagcc gattcgcgcg agaaaaccga gtgtatctgg ttctccgaac
    24001 gcagcgcaga ccagcgataa agcagcaacc cgaaagaaat gctgaacatg agaatgataa
    24061 acacgaagaa cgccgggcca atcgcagatt gagaaaaagc atgcactgat gaaagcatcc
    24121 cagaacgggt gagaaaagta cccaaaatga ccaggcaaaa agtgagaaca atgagggcga
    24181 tgttccaatg ctgaaaaagg cggcgctttt cctgggtgat ggctgaatgc aggtaagcag
    24241 tgcccgttag ccagggcatc agcgctgaaa tttccacagg atcccagccc cagtaccctc
    24301 cccaaccaag cacatcataa gcccagcggc tgcccagcac cagccccagt gaaaggaaga
    24361 gccacgccag caaagcccat ttgcgcgtgg atttgagcca gtgatcatcg gtacgaccgc
    24421 ttaccaatgc ggcgaaggcg taagcaaagg gaatcacaaa cccggtgaaa ccgaggtaaa
    24481 gcgccggcgg gtgaatgatc atgccgaaat gccgtaacaa cgggttcagc cccgtgccgt
    24541 ctgtgggctt agccaatatg gacatcgccg gtgggaagaa cgcgcgtttt atgatcccgt
    24601 caagggtaat gaaataacgc aaaaaaggat tttcaaaaaa gagtacaaga atgataaaga
    24661 acagcaacgt aaaggaagtg gccgcaatga cccagggcaa caggtctgcc tcgtttttcc
    24721 agcggcgcgc cacaactgga aaggtaaatc ctgcgagcag ccagcaccaa aacagcaacg
    24781 acccttcctg cccgccccaa agcgcggcga gcttgaggta gagcggcatg gataggtcgg
    24841 tggtttggta aacggaagag tattcgaaac gcccgctggc gagcaggtaa agcagcgcca
    24901 gtatggaaag agacagcacc gggaaggtga ttagcgcagc acgccgcgca gtttcaatca
    24961 gtgcatcaga gcgcgaacga cctccacaga gagaggttat caccgccgtc agtgtagtta
    25021 caagggcgat aactaacccg ccatacccga tacctccaat cacggcaccg cctgtgcagg
    25081 tacttccgct tcatagcgag agggacattt aagcaataac tcctcagcgt tgaacacccc
    25141 atcatcgccc atggcgccgg tcaatattgc ctgggcttca tttttcaaca ggtcaggcat
    25201 aacgccgtta tagcgcacct gtaagcgagc tgcctgggga tcgagcgagg cgttatgcag
    25261 cacattggcc aaccctccac ctgcttcgat ttcgcgattg tcaccgggaa tgtgcgcgat
    25321 ggtgaaggta aggtcaagag tttgagggtc atattggatg gtatcaccca gcaccacacc
    25381 cgaaacccgc acgttggcgt tattcacccc tttgccggat gccagttctt ctaccgtgag
    25441 gtaatactgg gcgttgcctt taaggctggt aatcaccagc agcaccaccg ccgccaggag
    25501 cagcataccg ccgatggcga acttatggat cttgaagcga gggtgaggtt caaccgcgcg
    25561 cgggcttttg ttcaagcgtt ctcctaaggg ttatgtttga aaaaaccaag ccactgtgag
    25621 ggcggttttt gttcggtgca tttattcgcg ctccgcaggc ggttcgggcg cctcgtggag
    25681 atctggagac tcgcgattta tatctgcctg attgtctttt ctgggttctt ctacctcttt
    25741 gattaaatcc gctactgagc gggtcagaga gctttggacc cccctggatt gacggtctaa
    25801 gtctctgatc gtttcatcga ggttggcttc cctggccagc attctgggaa tttcgtttat
    25861 ttcacgcctg gtcgcccata cttcctgcca gtatggtgac ctggtgatac ggcgaataaa
    25921 tgcgcccact tcccgggcgg acttcaccat attgccagga cccaggagta tcaacgccag
    25981 gacgacaatg aagatgatct caggcagacc aaggttgaag aatttcatat cacttttgct
    26041 cttgtccctg taaagggttg tgagagggcg ggcgtatcat tcctgcgttg agtcgtcttt
    26101 ttcttccttt tcttcacctt tcaggccttt ccgaaaagcc cggatggccc cgccaatctc
    26161 gccgccaatc ttactgatct tgccaacacc gaacagcagc gcgatgatga ccaggatgat
    26221 aatgagttct ggtactccga gtttggaaaa catatttcgc tccttgtctt gcgattattt
    26281 tactattagt tgcttcctgg tttgctggtt aaccagcacc ggcgccgtca gcctgctgag
    26341 atttttttcg gttatgcccg gcggcgaccg caaacaggat actgatcaga tagagggcgc
    26401 aaagaggaat catcatcaac cccatgttca ccggatctgg ggtgggggtg atgaacgcag
    26461 ccagaatagc aatgataacg atggcaatgc gccattgcct ggccagttgt ttggcagtga
    26521 ccagcccgat gcgcgccagt agaaaaacga taagcggcag ttcaaaagcc aatcccaccc
    26581 aaaagagcag gtttaaaaca aacccaaagt acgtgctggg ccggggagca gtggcgatgc
    26641 ccataaaatt cagtaaaaaa ggtagagcag tgggaagtaa aacgaagtag gcaaaaacca
    26701 cacctgcagc gaaaaagagg gtcgccagcg ggatcatgaa ccatacccac ttgcgttcgt
    26761 ttggtttcaa tcccggcaaa acaaatgcga agatttcgta caccacgatt ggtagcgcca
    26821 gcaccacgcc agcaagcagc gagattttca taaaggcggt cacattttcg gtgacatcga
    26881 tagatttcat cgccgagagt ccgccaattg gaccagccag aacctctgcc agttggcggc
    26941 tgaagacaaa gccaattaca gtggccaccg ccaaggcgat cacgctgatg agcaggcgct
    27001 tgcgcagctc attcacatgg ctcaatatgc tcattccaga agtttcttgt tccatttatc
    27061 caataacctt cgtttagctg ttaattgtaa cagggctata gggcagaaca aggacacttc
    27121 cctgtattta gcgtatcagc gctagcctgt ggaagtggat ggtgaattcg gtggcacgcc
    27181 cgttacaaca gacgaacagg ctggttttat gggttgtcat tattttaatg gtttcgcgtc
    27241 ttgcaggcgc ggtgaattca gtagtgggtg caactggcaa tcaaacaggg tgcggtcagg
    27301 tgagtgtgtt attcatcatg atagcgtttt tcgccggctt caacgcgcat cgccaggtga
    27361 ttctctggcg gcggcagggg gcaggtggcg taagcagtgt acgcgcaggg ccagttccac
    27421 gctaggttga aatccagtat cagccggttg tgaactggcc gccgggtgac caggtagcgc
    27481 ccgccggggt aggtggcgtc ttgcctggtg aggtctgtga aactgaagag cagttcatcc
    27541 ccctctgctt ggcccagcag cgtgcaattg acgccgctca tttgaaagcg ggcactccct
    27601 gggatgctga ccctcatttc actaccaacg gcggtgtgaa cgatcgtctc tctgggcggg
    27661 tcaaaaaaga tgaattctgc tgagactcgc caggcggggt tgactgcgaa ataattcaag
    27721 ccatgaaaac tcttcagccg ctgcgaatca cggtcccaaa cacgtaaaaa atacagcctg
    27781 ccgcgctgaa tgaccctcat ggctagcgaa gcgcattcca gcaagtcagg attttcgtcg
    27841 ttatcggtgc gcagctggcg ttcagcagca ggagcgccat taatcgtaaa gtggctgccc
    27901 acctctaccc agatttcatc tcctgtgagg gtgatcaaag cttcggcgcc ctgggcaagt
    27961 tccgggatgt ggatagtgta gcctgagcca tgacccaatc tgttttcgcc cgtttgcaac
    28021 gggtacagcc cgaccagtga aaaccagttc aagggggatt gcagcaggtt ctgttcacgc
    28081 ttccggcgtt ctctctgaat catcttctca tacgaggtgt gtgttttcat gggtaaccgt
    28141 tcaggcgtat tatttttagc gttaactttt aggagtgatc gaaaaggaag cagcaggatg
    28201 gttaagtcct gctgcttgta ccgatcactc gtattcaata ggcttgtggg gttcttcggg
    28261 ttctgtttac cccaattgaa tactggttcc gggtcatttg ataaaaatca atagaaccgg
    28321 atcgtgggat gggtatactt cactcagtcg agcagttcct tcggcacgtt gccgccgttg
    28381 tccgcgatct tctgaaggac aactttgtgc agccacatgt tcatttgggc tgaatcagcc
    28441 atcttttcct ctgggcagcc aagttccttg gccagttcct tgcgttcagc aaggctgctg
    28501 tcaaggccca agagtttcat caggtctacg atcgacgtct tccaatcaag tttctctgga
    28561 tttgcggcgg ccagtttttc aagtttggcg accacatcca ccatggggac tgccgcgggg
    28621 gcacccggct tgaaagcatc gagcttaaag ccggagcctg cggctccaac catccctgcc
    28681 actgggcggg aatccttcgc cactgagccg cctggtttaa gcgcgctggt cttcgggatt
    28741 tcaggtttta cggcaggtgc agcagcggct gctttttcct ccttaccctt gatgcccaac
    28801 ttgtcgagaa ttttagagaa tactcccatt tttattcctt tccactcatt ttaatcagaa
    28861 ccccaactag ggttaattat agacggtttt tgcaagaaaa gttaatatta accagccaat
    28921 tttcatctgt tattttataa aatcattcat accataaagc ccacttcacc atccaccctg
    28981 tgtattctcc tggaatttca ttgcaacgct accgcggttc aagcgtatac tcatggtata
    29041 gatgataaaa tcgtttcgaa aatcacagca ataaggagaa gaaaagatgg gagaggtagg
    29101 caagaaagac aagggcaaaa aagaacaaaa gaaaaaaccg aagttaaata tcaaggaaaa
    29161 gagaaaacag aaacaggaga aaaagagcgg taccgcttaa tgaacttaag cgcagcgctg
    29221 gcttttaacc tgctttgcgg gcaactcaat catctggtaa ttccggtcat cctcatggct
    29281 ggagtagtat tccgttgcct gatattcctg cacgattctt gaatcagtcc ccatccgttt
    29341 ttcacccgct taactgaccg gcttatgatg aagacccata tccgtaacgc catcctgctg
    29401 acccctcagc aacgaatgga acataccgtt ctcactctga gcggtggggt tatcgagaaa
    29461 ctgaccccgg aggaggaatt ctcatttgag cccgggaata cgctcgacgc gaagggctgt
    29521 tatgtcgcgc cggggttcat tgacttacat tttcatggcg ccatgggggt tgatacaatg
    29581 gacgcagtag ctgcgggtat ggtgaccgca tcagcgtatt gctctcaaca cggcgtaacc
    29641 actttttacc ccaccacctg gagcgccagc caggcagaaa ttacacgtgt gatcaactgt
    29701 gtgaaggagt gcgccagtca cttacccggc gcgcgtgttg gcggggttca cctggaaggt
    29761 ccctacatca acccacgcag gcggggagcg cagtcactct cttttatccg taaaccagac
    29821 gcgcgtgagt acctgccctg gctggagtcc ggtatggtca gcattctcac ctgcgcgcca
    29881 gaagtggaag gtggcgaagc gttgattcgg aacgcggtgc ggttgggggc gcgtgtggcc
    29941 atcgggcatt ccagcgccag ctacgagcag gtgaaagccg ccgcgaacct cggcgcgacg
    30001 caggcagctc acctgttcaa cggtatggat ggactgcatc accgccaccc aggcacagcc
    30061 ggaggtattc tggatgatga ccgtttgctg gcgcaggtga tttgtgatgg cgttcatctt
    30121 caccccgcgg tagtgaggct tgttctcaaa gccaaatcac cttcccgcat cgcgttgatc
    30181 acggattcca tcaggggagc agggcgcgct gacgcaaagt atgagggtaa cgggcagttc
    30241 atccaggtga aagacggggt cgcccgtgat gctgagggtc acctgtccgg cagtacaatc
    30301 tctatggact tggcgctgcg cagcctgatc gccttcacgg gctgtgattt gctggaggct
    30361 ttggtgatgg ttaccagcgc cccggcggaa gagatgggct taggcgcgaa aaaaggaagg
    30421 ttggcaccgg gttatgacgc ggacctggtt ttcttgcaaa aagacttaac ggttgccgcg
    30481 acgatggtct cgggcgcttt cgtttaccag tgctgaacaa caatcaatac acccgcaaga
    30541 taggcggcgg gaagacatcc tcaaccccgg gaggtagcat acctcccggg gttattttat
    30601 gcctgctatt cgatctccga tagtttgacc ggtcggtttt ccaacatcga gcgggtggct
    30661 gccatggcaa tgaccaccgg tttgcgccca tcgaacacgt tcaccggggt cgggttgccc
    30721 tcgcggatag cgttaataaa atcgcgcatt tccgccacga acgattccat gtagcgctcc
    30781 aggaagaaat agagtggttt ttctgaaacc acaccatccg cattgctgta cacgctggag
    30841 ttgggggtat tgttggcagt agcggccatg ccaccagaac caaaaacttc cgcgcgctgg
    30901 tcgtaaccat aaaccgcttt gcggctgtta tcaatcgtgc cgatggcgcc gttggcaaac
    30961 ttgagcgtga taacagcggt atcgatatca cccgccgcgc caatagccgg gtcgaccctc
    31021 acctcgccga tcgcatacac ttcttctacc tcgctgccgg agagaaaacg cgccatgtca
    31081 aaatcatgaa tggtcatatc caggaacatg ccgccggaga gcttcacgta ctccgctgga
    31141 gggggggccg ggtcgcggct ggtgatacgc aggatatgca ggtcgccgat tttgcctgtt
    31201 tgaaccaggt cgtgcagctt tttaaagttg gggtcaaatc ggcgattgaa gccaatttgg
    31261 cattgtaccc ccgccttact gaccgcgttg atggctttat cgatcctatc caggctgtgg
    31321 tctaccggct tctcgcaaaa cacgtgcttg ccggcattgg cagcggcgat gatgagatca
    31381 gcgtgggtgt cggtagaaga gcacaccgcc actgcatcta tgtccttgtc ttcgatgatc
    31441 tttcgataat ctgaaaaagc gttggcgacg tgcagcttct ccgccagcgc ctgagccgcc
    31501 tgctggttta tatcagcaat cgcggaaacg accacgccag ggatgcgcgt ggccaggttt
    31561 tcagcgtgaa tttttccgat gcgcccggcg ccaatgaccc ctactcgaat taccttttga
    31621 ttgctcattc ttctccttga tcaattgaac ccgatggatt tgaggtattc acgatttcta
    31681 agcgctgatt cgtagggact gcccatgccg ggtaacacat cctgttccac cacgatccag
    31741 ccttcaaatc cctgccgttc cagctcagcc ttcaccgccg ggaagtcaac cgcgcctttg
    31801 cccagttcgc agaaaacacc ttgctcgacg gacttaaaat aatcccaccc cagcacgcgt
    31861 gatcgttcag caataccggg atggcaatcc ttaaagtgca tgtgccatac cctgccagcg
    31921 ttttcttgta aaaaagtcag cgggtcacca ccgccgaatt gataatgacc ggtatccagg
    31981 caaagcccaa ccagttgtgg gtctgtgagc cttagcagcg agtcaatctc agccggggtc
    32041 tcaacgtatc cgccgcagtg atgatgaaag accaaccgca acccagtctc tcttttgaca
    32101 gcatctgcga ccttctcagt gccttcagca aacacctgcc attcagcatc gctgagcccc
    32161 atctcagggg tcacacgccc agcattgcgt gtgcgctccg gcaccttgcc attgtcatcc
    32221 gccagcacga tgaaagggga actgccttcc accgctgcca gcagcctggc aacctttaag
    32281 gcgctttcaa tgccctgtgg gtgggtagta gcgtctttaa gcgccaccgg tacaaatgcg
    32341 cccagaagca ccagctcacg ctttttcaat tcttctttta attttccagg ctctgtcggc
    32401 ataaaacccc agtcacccag ttcagtaccg gcgtaaccgg agcgctgcat ttcatcgagc
    32461 acgcgttcat agccggcagc tttgccctgc aggtcgaatt ccagtgctcc ccagctgcat
    32521 ggtgcgtttg ctattctgat catacttcct ccaaaaaaga taaggtgaat tacaggtcca
    32581 cttcacgcac ctgcaccggg cgtccttcat ccaacgaaag ggtggcggca atgccaatag
    32641 cggtagccgc gcgcgcgtct tctccggtaa cctcgggcgg tgtgtcttcc aaaatgtgct
    32701 tcacgaacac ccgcacctgc tcggcgtagg ccaagccaaa ccgctccata aagtaaggca
    32761 ctgtatcgtg catgacccct gcgcgggtca gcaccagggt tggggtttgt tgcaggtagc
    32821 cgatccaaag gctgccatct gtgcccagta cctcggtgcg gatgtcgtaa ccataaacgg
    32881 cgttgcggct taaatcgatg ttgccaatcg catcattggt aaagcgcaga ttgatgacaa
    32941 cattgtcgat atctcccacc tctttaagct ctggaaaagc caggcaggcg ccttcgctat
    33001 gcacacgggc gacttcgctg tccatcagcc aacgcgctaa atcgaaatcg tgcacgccca
    33061 tatccaggat caacccgccg ctattctcac gtttggcaaa ctccaggctg gtgcgcttgg
    33121 ggtcgcggct ggtggatttg aacatcaccg gtttaccgat gacgccatcg cgtatttttt
    33181 gtttcgctgc ggcgtaaggg gcgtcaaagc ggcgcataaa gcccatctgc agtttaaccc
    33241 cggcctgcct gaccgctgtc agcgcatcat cgcaacgctg aaggttctgc gaaagcggtt
    33301 tttcgctaaa gatatgcttg cccgcctgcg ccgcggcggc gatcacttcg gcgtgcgtgc
    33361 cggtgggaga ggccacaaca acggcctcga tctcctggct gtctaacagg tcgcgataat
    33421 cttggtaagt ggaagggatg tggtagcgat ccgccacctc ggcgagcgca gcgcggtcag
    33481 gatcagccac tgcaaccagg tccacttcgg atactgagaa cgccagcgtg ctggcataaa
    33541 ccctgcccat tcttcctgcg ccgatcaatc ctatcttcac cgccatcgtt cacctcgcgc
    33601 tttttctatc tccatatcaa aggaaatatt tctcgtcttt caggtgctcc tcgtaatctt
    33661 tacgcgccgc ctgaacctca ggcatggttg aaacctctgc cacagccacg tcccaccagg
    33721 attcgtaacc cggtaccctc acctcgcggt ctgctttaat gacaatcacc gaggtatggt
    33781 caagtgacct ggtatgagcg agagctttac gaaagtcctc gatggaacca ggtgtaaata
    33841 cctcagcgcc caggctggcg gcgttggcag caaaatccac ctccagcgca gggccattaa
    33901 gctgaccggt ttgcgggtca cgtttcctaa aacgagttcc gaagccagag ctgcccaaag
    33961 aggcactcaa gccgcctatc gacccaaaac cagcgttatc gaccacgatg atggttacct
    34021 tcaatccctc ctgcgcagcg gtgaggatct cggtgggatt catcaggtag gtgccatcgc
    34081 ccacccacac gtacacctcg cgctcaggcg ctgccagctt cacacccagc gaggcaggaa
    34141 tttcatatcc catgcaggag taaccatact ccatgtgata ggagttgggc gtgcgcgcac
    34201 gccacaactt gtgcaggtcg ccgggttggc tgcccgcggc cgacatcatc acatcgccgt
    34261 cggcaatcgt ttcccatatg gcgccaatga tctcgctctg agcgggtaaa ggctggtgac
    34321 ggaggtgata caaacggtcg gtttcaactt cccattcgtg tttgagttcg cgaatgcggg
    34381 tggcgtaatc ggctgatacc tggtagccag cgagagccga ggtcaattct tccagcgtcg
    34441 cacgcgcgtc tgcggtgagc gccaaccctg aatgcttaaa agcgtccatc tcaaacaggt
    34501 tgatattgat gaagcgcact gcgagattct gaaaagcggt tttactggca gtggtgaaat
    34561 cagagtatcg tgtgccaatg ccaatcacca agtcggcgcc atgcgcgatg cggttggcgg
    34621 gcaacgtgcc agtggcgcca atcgcaccca ggcactgggg gtggtcataa gccagtgaac
    34681 ccttgcccgc ctgagtctct cccacgggaa tgccggtttg cctggcgaag gtgtcaagcg
    34741 cctggctggc gccgctgaac agcaccccac cgccggcgac gatcagtggc tgtttggatg
    34801 aacgaatcat ttcaaccgcg cgttttaaaa gcgcgcggtc tgccagcggg cgcgcgacca
    34861 tccaaacacg tttgcggaag aattcaagcg ggtagtcgta ggcttcagcc tgcacatcct
    34921 gcggcagcga gagcgtcacc gctccggtat ccgcagggga ggtgagcacg cgcagggctt
    34981 caggtaaaga ggtgagcagt tggtcagggc gggtgatacg gtcccagtag cgcgatacag
    35041 gtttgaagca atcattcacg ctgatgtcct gcgtattggg agattccagc tgttgcagca
    35101 ctggggccac gttacgacgc gcaaaaatat ctccgggcag cagcagcaca ggcaggcggt
    35161 tgatggtggc caacgccgcc ccggtgacca tgttggtggc gccaggacca atggacgatg
    35221 tgcaggcgaa ggtttgcagg cggttgtgca ttttggtaaa gccactggcg atatgcacag
    35281 cggattgttc gttacgtacc atgatgtagc gaaaatcagg gttttcttgc agcgcctggc
    35341 cgatacctgc cacgttgccg tgaccaaaga ttcccagcat accagcgaag aaaggggttt
    35401 caactccgtc ccgttcgata tactggttct taaggaagcg gacaagggct tgcgccattg
    35461 taagtcggat ggtggaggtc atttctctcc tttcaacggg ctacagatcg gggttgggga
    35521 gcatgaaatc gcgtactact ccctgcagtt catggtaaga actgataaat ccgcgcaagg
    35581 ttctcacagt ggcaccgtag gtgtcaaatt gcgctactgt catcccatcc ggctcgtacg
    35641 ccttgcggaa ttcaggtatt ttgctgagca gcgccttaac gatgacgggg tcgactggat
    35701 ctgccatgcg cggacgaact tcgatatcgc tggcgttaat cttgagctgc cattcataag
    35761 ggatggtgag gatgacatca ccgccaatga actcagacca gtgtcccagg tggcgataag
    35821 ctgctgccag cagcctgggg cggtaccctt tttccttgaa aatttcataa gcgcgtttaa
    35881 agcaggcgat acccgcccaa tcgaggaaag cagggtcaat atcaaccgcg ttctttttgg
    35941 ccgctacctt catccagtca tccgtgcgcc ccaccattat ggtgcatacg ggcgacatct
    36001 cagcgatgga tttcccttca gcgctgcgcc gctcaagccc ccgttccatg gcttgcgcca
    36061 ccgccaaagc ctgggaaacg ctgaaacaca cggtggcgtt gatggatacc ccgtggtaag
    36121 tggcttcttc gatcgcttta acgccggcgc tggtaaccgg ggctttcacc tgcatattgg
    36181 gtgccagggt ggcaaagtga atggcatgtt cggcaatgcc cctggcgttg cggtaaagct
    36241 gcgggttagt ctgaatggaa agccgacctt tcttccctcg ttcacggtca aaagcaggtt
    36301 taagcaaccc ggctccgcgc acagccattt cctcgatcaa tttccaggtg atctccacct
    36361 cagaccagat cgggttttcc gcaatgatct gatgaatacg ctcttcccac aggttcattt
    36421 cctttttaag cacattggcc acgattgtgg ggttggtagt ggcaccaacc gcgccgtgtt
    36481 caaccgcgta agttaattct tctatggaac aggagtcatt ccagtaatcg gtgggggtcg
    36541 ttgagactgt ctcgtgcaat ttgctcttat aggtggtcat ttttcactcc tttacttgta
    36601 gttcatggat aattggacaa gcggcaggcg cggatccttg ctcttccagg tgtctttgat
    36661 ccaggcgtat gccgggtcat cgctgctggc gagagactgg tctgagcccg cgagaatgtt
    36721 caggtagtaa gccgtgtaac cgtgggcagc caccacgggg tgatatccct cgggtgataa
    36781 gacgaggtgg tcattttgca ccagcataat ttcgtcgatg cggcggtcat ctgtgtaaca
    36841 gcgctggtaa gcgtaacctt caggtttatc gattttgtaa aaataaattt cttccaggtc
    36901 agcctccagc agccggccat tttcatccac gcgacgcaca tcgtgcttgt gcggtgggta
    36961 agagctccag ttgccagatg gcgtgtaaac ctctaccacc accaggcgct cacacggaaa
    37021 acccggaaga atcatctggt tgatctggcg tgaagcgttc tcgccgccgc gcagttcaac
    37081 tttcacctct tcagggctga ccaaccgcgc cggaaaatct tttgtagcgg cagcccagcc
    37141 gcaggcgaaa tcgaccgcac cgtaagcggc cgtaacggtg aaatcggtat cgcgggatag
    37201 gtaaagggtg gtaggcatgc catgaaacac atctgtccgg ctgccaatgg atcgccacgc
    37261 tccgcggttc gagtgcacat caagcgttcc gccaaggatg accagcgcca gttcgttgtt
    37321 ttcagtatta aaatgccatt cctcgccttg tttaagcctg acagcgcaga aagacaaatg
    37381 ttcccagcca acgctttgcg gggtcacccg gatatactct gcacgggtga gatctggttt
    37441 aaccagtaaa gaagattcgg tagatttcac tttttactcc tttgcaaatt gagcggtcga
    37501 ttttcttacc agcaattcac cgcgcatcat cttattgaga ggttttctgc gcgactgtga
    37561 tgaagcattg gagctgataa gttccaacaa catgctggcg gcctcatcac ctatcgagcg
    37621 tttgggctgg tcgaaggtgg ttaaagcggg agtggtgtag gctgaatagg ggatgttatc
    37681 aaaaccagcg atggaaatat cttcagggac ccgcagccca ttttgtcgca acacgctcag
    37741 cgcgccgact gccatgaggt cgttaaagca gaatacggct gaaaagggaa caccggcgcc
    37801 gaggagggcg gtcataccgg ccgcgccgtt ttcaatttca ccaccaggtt ggtgggtaat
    37861 ccagttttct gccaccgtaa aacccgatga ctcgcattca gcctttaatc cctgtaagcg
    37921 gttatcgttg atgcgcggag cgcaggcgtt tcccagataa gcgatgcgtc ggtggccgag
    37981 gtcaagcagg tggcgcgtga cctgcctggc gccatattca tcatcgtgcg aaatcgagta
    38041 tttttgcgaa cgcgcagact ggttattgat gaccactgcc gggattccat actggttaag
    38101 caggttgaga ttgctgcgct caaccggtac cgaaccaatg atgaccccgt ccacccgatg
    38161 ttcactgaag gcttgaacaa tctctttttc tttttgtggg tcgagagaag acgaggcaat
    38221 gaaaatgctg tatccgctat ttctgatggc gtcttcaatg ccctggatga tctcgccaaa
    38281 atagggattg tcaatgcggc tgaccagcac ccctaacacc ccggaacgcc tggttttaag
    38341 cccccgggca gcggcactgg gcaggtagcc caggtctccg gcgatattcc ggatcatggc
    38401 ggcggttttg ggcgggatga cagggttgcc attgagcgcc cgcgagactg tggtgtgtga
    38461 aacgccagcg acctttgcaa tatcttttat cgtgacaccc ataacactcc attagcgcac
    38521 acgtgtgcat aaacatcata gcaaattgcc ttatgaatgt caacttaaaa aatgtaagaa
    38581 tacttgcaaa attaatttaa atgttgtatt atcatacatg taagtaatcc tgcaaaaaac
    38641 attgaaatgt taggccagca atgcatttcg cctgagcctc accgccctgg acacgcctga
    38701 acttctataa gaatcgacct cgtgaaagga tatggatatg aaccctgaca tcgaagccat
    38761 ctttctggac gtaggcaata cgttacgcat cgtgctggaa gagcctgatt tccaacagaa
    38821 cgccatgcag gagttaatgc gcctggttgg aacgcaagaa tcccaggaag ccttctttga
    38881 aaaactggag acccgctgga aagcttaccg taaagcctcc aaggcgtctt tgctggaatc
    38941 gcccgaggaa attttatgga caaaacatct gctgcccgac tacccggcgg aaaagatagc
    39001 ccccctgcac ggcaggctaa cgcgcctttg gcgcgaccgg gatggacggc gcgttccgcg
    39061 gcacgacgcc aaagagacca tcattgagct gcaccgccgg ggttacctgc tgggcattat
    39121 cgccaacacc atcactgaaa cggagattcc cgattggatg gaagcggatg gtgtgaccca
    39181 gtatttcaaa accgtcatcc tttcctccaa agtgggcatt cgcaaaccag accccgaaat
    39241 ctattggatg gcagcgcgcc aaattggcgt ggaacccgcc aaatgcgcct acctgggcga
    39301 caacccggtg cgcgatgtgg aaggaacgcg cgcggccggg ttcggtatga tgatcctttt
    39361 cgatgaaccc gccacactgg caaaagaacc acccacgggt gagcacactc ccgattacac
    39421 catcaaagag atcagggaac tattagatat cttcccgccg cgaaaccgcc aggtgaatta
    39481 aagcacccac atggatatcg acgccatttt tctcgacatc ggcaacacct tacggatatt
    39541 gatgaaagaa gagccgtatc gtgaagaagc gcgtcagaaa attgctgcgc tggttggcac
    39601 aagccaaccg ccgcaatcgt tcagcgagga gctggatcgg cgttacaagg aataccgcaa
    39661 gtgggctttt gaacacctgg ttgaggcttc agaagaagaa ttgtggacgc gctggctggc
    39721 gcctgagttc ccagcccaac acatcgcgcc actggcgcac gaactcacct accagttccg
    39781 ccaatccatg gggcggcggg tgatggcgga tggctgcaag gaagtcattc atgaattgca
    39841 cagccgcggt tacacgctgg gaatcatcag caatgtcatc actaccagcg agattcccga
    39901 ttggctcgcc gctgagaatc tttcgcggta tttcaaatcc gtccttctct cctctacctt
    39961 cgggcggcgt aaaccagatc catccatcta tctggaagcc gcccgaaggg ctgaagttgc
    40021 accagcaaaa tgcgcctacg tgggcgacaa tttctctcgt gatgtagctg gaaccagggc
    40081 agccggcttt ggcatggtga tcatcctgcc agacccggcg gaaaaagacc tcagggttgc
    40141 gccggagcaa caaccagatc tggtgattca cagcctgtgg gacttgctgg atcactttcc
    40201 gcccagaaat ggacaccagg agtaaatatg ccgaaatcag caggtatttc actcgaaaac
    40261 acaaatcgcg ccagaggaag tgcggcgctg gcaaaaacgg tgaaggacct gtacgagcgg
    40321 gggcaaaccg attacaacat ggagccgatc atcctgcaag acgcggcagg aaatgcggtc
    40381 ggtcggatca aagatggcga tgcggtggtc ttctgctgcc gccgtggaga gcgtgaaatc
    40441 caactgaccg aagcctttac tgacccccag ctgaaccaat tcccgcgtga ggtgttcacc
    40501 catctcccct ttgtgattct cactctgtat catgacaagt ttaaagacct gccggtggcg
    40561 ttcgcgcctt caaaagtcca ggcgaccctg ggcgaggtag tgagccaggc gggtttttca
    40621 caggtgcgca ttgctgaatc agaaaaattc gcccacgtga cctttttttt gaacggcggc
    40681 aacaaccagc catacccaca agaagaagat atccgtatac cttctttgaa aggcgttcct
    40741 tttgatcaat acccggaatt aagcctggag aaggttgcac aggctgtcat agaagccatt
    40801 cacaggggga caggcctggt ggtgaccaat tttgcgaatg gggatgtcat cgggcacact
    40861 tccaataaag cagcgaagat cgcctgcgcg cagtttgtag accggcacct gcgcgaagtg
    40921 gtagaaaagg ctaacgcggc gggttatgtg gtcatcatca ccgcggacca cggcaacctg
    40981 gaagtgttgg aaaacgcgga cggttcagcg catgtttctc acaccaccaa cccggtctct
    41041 tgtctgatca tcgatccggc agccgcggag acggtcaccc cgcgtgaggg caagctggcg
    41101 gacatcgctc cgacagtgtt gagcatcctg cgggtaaccc cgccggcgca aatgacagga
    41161 aagaacctgg cgccggagca tgactttggg gttgaccgta aagtcttgct ggtcatcctg
    41221 gacggttggg gaatcagcgg cgttcctgag aacgatccaa ttgctatggc ggagacgcct
    41281 ttctgggata gcctgctggc gcgttacccg cattcgctcc tggaggcctc aggtgaagcc
    41341 gtggggctga aagccggcaa agcaggaaac tcagaagctg gacacatcaa cattggcacc
    41401 ggcagggtcg ttttgcagga tgatgtgcgc ctggataggg cgatgcagga tggctctttc
    41461 tacaccaacg aggtcatctg tgacgtcatt gaaggcgtaa aagcgcgcgg gaagaacctc
    41521 cacctcatcg ggcttttgac tgaaaaaagc tcgcacggct ccatggaata ccccctggcg
    41581 gttttgaaaa tggccaaaga cgccggcttg cagcatgttt tcctgcatat gatattggat
    41641 ggacgcagca ctgagcccgg aagcgcgccc gcgcttttga agaaactgga agatttaatg
    41701 aatgaaatcg gcgtcgggca gatcgtcacg ggtattggac gcggtatcgc cctcgaccgc
    41761 gacggtaatt acaataagac gcaaatcgcc tacgacgcgc tggtctatgg aaaaggcagg
    41821 ggagccggta aatgaactac gaattcctca agaaactgat ccaaccggcg gataccaaaa
    41881 tcgttatgct catcatggat gggctaggag ggctacctaa agaacccggc ggtaaaactg
    41941 aactggaaac agctcatacc cctcatatgg atgccctggc tgctcggtcg gcattgggct
    42001 tttccagccc tgcgggggcg ggcatcgcag tgggcagcgg gcctgggcac ctggccatct
    42061 tcggttttga cccgatagag cacgagattg gccgcggcgc gctggaagcc ttaggggttg
    42121 atattgacct gggggcggag gatgtcgctg cccgcggcaa cttctgcaag gtggacgcca
    42181 atggcatact aacagaccga agagcggggc ggctcgcaac ccctgaagcc ttcaggctgg
    42241 caaagatgct aaacagcatc gccatcgacg gcgttcagat acaggtcgag ccaatcaaag
    42301 aacatcgttt cgcgctggtc atgcggggcg agggtttggc ggcgaagatt tcagacaccg
    42361 acccgctggt ggagggcgtt gcgcccaggc aagcggttgc gctggaccag gcttctgaaa
    42421 aagccgcgcg gttggtcaat gcgtacatcc aaaaagcgca agaaatcctc cgttctgaag
    42481 aaccggcaaa tatggtgctt ttacgcgggt ttgacaaact gcccgtgctc ccaaagtacg
    42541 gggagctttt caagttgtgc gccgcttcta tagccgtgaa cggcatgtat aaaggcgttt
    42601 ccaggctggt aggcatgcag gtattaccgg tggatggcgt aaccatcgag gatgaattct
    42661 caacgctgga gatgtactgg gagcagtttg atttctttta cctgcacatt aaacaaaccg
    42721 atacccatgg cgaagatgga gacttcgacc gnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    42841 nnnnnnnnnn naaagagatg gattttaccc ggcttatcag tgaacaatat caaacgctca
    42901 ctaagagcga aaaaatgatc gccaactttt tacgcaaaaa ccaggaggag agcgcgttcc
    42961 tttctgcggg tgagatcgcc aggcgcctgg atttgagtga ggcgacgctg gtacgtttcg
    43021 cgcgcacact tggctttaac agttaccccg caatgcggtc cttactgcaa gaaaacttcc
    43081 gccagcgtat cacccattcc gcccgcttac gcggcaggtt ggatgagatg cgccagaatg
    43141 gcggcatttt tgaacgcgta accgctacgg agatcgacta tctcacccag gcgctgaatt
    43201 caatagaccg cgatcagttc aacaaggctg tggagctgat taaaacaaat gagaaggtct
    43261 atgtgtttgg cgttggaccc tcgatcacgc tggtagacct gatggaaatc aggctgcgcc
    43321 gttttggcaa agaagtggtt ccgctcaaag cgtacggtcg ggaagtcgtc gacttcatgt
    43381 tggctatgaa acccggtgac ctggtttttg tcatttgttt ctttgaccaa aaccccacgc
    43441 tgcacctggt gctggattac agccgcaaag ttggcgccac ggtgattatg ttgacggaca
    43501 cgctggagac catattggga gactcggcag atgtgatttt aagcgcgcgt cgtggaccgg
    43561 ttggggcgtt ccactcgctg gtggtgccta tgacggtgat caacgcgctg ctgctttcag
    43621 ttgccagttt agaccaggag cggattatgc cagcactgga taaattggat tccctgcgcg
    43681 gtcagctgaa ggaattaaac cgggcaggat gattcgtttg tgtgaatctt acggcatgat
    43741 tcatgttcaa cacttgatga agaaaggaaa tcaacacaag gtgcaagctg atgacagtaa
    43801 catcaattga accaaaggac aacaaggaaa agtggctggc tcgcctgcgt cgctttttcc
    43861 gctggttgac ctcgccgcat gtggttcttt ccatcatcat ggttgcggtg atgttctacc
    43921 tggtcatcat accgctttat cgcatggtcg tcactactat cacggtggcg gatgttgacc
    43981 tgagggcgat cagaggtgct gaagaaggcg actttacctg gtatcactgg gtgagaatgc
    44041 tcacaggcaa gatcggcaaa ctgatgactt atcaaccagc gatgcatagc atagtggttg
    44101 ccctgggagc aaccttcctg gcgttagcgg ttggcgggtt gatggcgtgg ctggtgtgcc
    44161 gcacagatat cccgggacgc gataccatac atatgctggc gcaggtgccc tatatcatgc
    44221 cttcgtggac catcgcgatg gcctggctgg tgctctttaa gaaccagaaa tcagggggca
    44281 ccatggggct gctggaattt attactggcg cgcccacacc agactggttg gcgtatggcc
    44341 caattcccat cattgtgagc agcgggttgc attactacac cttctttttc ctgttcatcg
    44401 cggcggcgct cctgtcgatt gattcatcgc ttgaagaagc gggcgagctg gcgggagcgg
    44461 ggcgctggcg cattatgcgg aaaattactt tcccgctggt tatccctgct atcgcatcag
    44521 gtttcattat gaccttttcg aaggccatgg gcacctttgg cggaccgaac gtcctggggg
    44581 tgcctgtccg ttattacacc ctttcgacca tgttgagatc caattcttcc atcggcaact
    44641 atggcgacgc ctttgtgacc gccattgtgc tgatcttgtt tgccatggtc accgtgatgt
    44701 taaatcaaaa gatcgtgggc acgcgaaaaa gttatgaaac gatcggtggt cgtgggttta
    44761 tgtcgaagaa gaccaagttg agaaatttta agccaattat cgtcgctatc gtggtcatct
    44821 ttcagttcat tatcgctgtg ctgcccctgg tgctgctggt ctacagcaca ttcatgctga
    44881 gagatggaaa atggacgctg gacaacttca ctctcgctca ctggattgga aagggcgatc
    44941 tctcgatcaa cagcggtgaa ccgggcatcc tgctcaatca caaggtttgg ttgggtgcct
    45001 ggaactccgt tcgtttgtcc atccttaccg ccctcttcac cgcgcttttt ggagtggtgt
    45061 tagggtacgc gattgtcaaa gggcgcggaa cctggctttc gaaaatcgtg gaacagatgt
    45121 cctttatccc ttacgtcatc ccgggcatcg cttttggcgc ggtatacatc gccatgttca
    45181 ccaaacctgt tggccccatc ccggcgctgt acggcacctt cgcgttgttg gtggtggttt
    45241 cggtagccaa gcacctgcct ttctcagcgc gcagcggtgt ttctgccatg atgcaggtga
    45301 gcaaagaact ggaagaggca gcaatgctgg ctggtgcgtc tccctggagg cgtttcgcgc
    45361 gcatcgtctt tccgctcacc agcaccggtt ttgtttctgg ttttctactg acattcatca
    45421 ccacgatgcg cgaactctct ctcattatcc tgctggtcac ccccaccaca gcagtgctgg
    45481 cgagcatgac catgcggtac attgaaaatg gcaacgacca acaggcgaat gccgtcatta
    45541 tcttgttgat cgtgctggta ctgatcggca actttattat tagccgcttc cgtggcggca
    45601 gtctgaaaaa agggctggga ctgtaaagag gagaagaaat ggatacccca atcgtcatta
    45661 ataacctcac caagaagttt ggcgatgtca tcgccgtcaa taatgtttca ttgcgtattg
    45721 aacctcacac atttttaacg ctgctcggtc cttctggctg cggtaaaacc accctgctgc
    45781 gctgtatttc cggtcttgaa gacccggatg agggcgagat atatattggt gatacgctta
    45841 tctattctca tgcgaaaggg atatctgtgc cgcccgggca gcgggaactg ggaatggtct
    45901 ttcagaacta cgctttgtgg ccgcacatga cggtgtataa aaacatgacc ttcgctttgg
    45961 aaattcagaa actgcccaag gctgaaatgg acaagcgcgt gaaagaatca cttgaagagg
    46021 tgaagatgag cgggtatgaa aaccgctacc cgcgtgagat gagcggcggt caacagcagc
    46081 gtattgcgct ggcgcgtatg ctggcttacc gcccgaaggt ctttttgatg gatgaacctc
    46141 tttccaatct ggacgctcgt ttgcgtatgg acatgcgcac cgagctgaaa cgcctgcacc
    46201 atatttcaga cgccaccacc gtgtacgtga cacatgacca actggaagcc atgaccatgt
    46261 ccaccaacat cgccgtcatg aaagaagggg tcattcaaca aatggacacg ccagacatga
    46321 tctatcatta ccccgccaac ctgtttgtgg ctgattttat cggtaacccg aagatcaacc
    46381 ttctgaaagg ctgggtttcg agtgaggatg tggttgacct gggtaagttc aacatccccg
    46441 cgtatacaca cagaaagacc ggtgatgtgg tggcagcttt ccgcccggag gacatcacta
    46501 tccacactga gccggttgaa ggtggggtgg aattcagcgc ttactctgta caaccatccg
    46561 gtgccgatac cacgatcatc gcgcacaaag gcgataccga gatcattgtc aaagaaatgg
    46621 gtgtcagtaa gattgccatg gaccagaaga tctggttgac ctttaataaa gaggccatta
    46681 acctttatga taaggcgaca gaaaaattga taacaggttc gtgaattaat gtcaggtgaa
    46741 ataaaaaaag gaggaggtga tcgtatcgtc ttttggtgtc gttttctgtc tgttgaatgt
    46801 tcaaaataaa caaggaggaa ggtcaatgaa agcagtaatt cgaattgcaa cacttgtatt
    46861 gttggttagc ttcctggcgg cctgcgcgaa acctactgcg gctcccgtgg ttgaaccgcc
    46921 ggtggttgaa gaacccgtcg taacggaagc ccctgtggtg gaggaattga cagcccaaca
    46981 gcagtggttg aaagacaacc agcttggcga atactacacc gacgaacagg actgggcggc
    47041 catagaggcg gcggcgattg cagaaggtga agtgctggtg tacgcgaaca catccaaaat
    47101 tgaaaaatcg gcaaaagcct tcatggaaaa ataccccggg atcaatgtgc aggcgtttga
    47161 cctgggcgga gatgatgtgc tgctaaaaac ggtagaagaa cagaaggctg gcgccttcac
    47221 tggtgacgtg tggtcaagca gcggtggggc tgaattggtt gggaatgtgt tgcctaaggg
    47281 ttatgtatgg cgatttatcc cctccactgt tgcggatagg attccagagc aatatactca
    47341 tcctctcttg atggcacgat tgggcaccgc cgtgttcgcg tacaactcag aattaaacga
    47401 tacatgcccc atcaccaaca tctgggagct cacacaacct gaatggaaga acaagttctt
    47461 cattgaagac ccgatcaacg atgcttcaac actcagcaaa ctcctcaccc tggtaagtca
    47521 ttctgaagaa atggccgcag cctatgttga gctgtatggc accgagccag ttctggaaga
    47581 ggatactcct gacgcgggtt ggctgtggct gaaaaaattc gctcagaatg gaccaatccc
    47641 gcagccaggc ggcgatgagg tggatgaggc gttcgctact cctggaatga aagaaagcta
    47701 tatggccatc acttcctact ccaacatggc cgatgtgatg gaaggcaacc tggtatttga
    47761 accctgttgg ggaatcaaac ccatccttgg tattcagagc cagagttacc tgggcatcat
    47821 caaccaggcg ccccacccca acgcggccaa actttggatc gcctggcagg tgagcgaaga
    47881 aggccgcaaa ccctgggcga aattcggaac ttacttcaca gattccactt atgaagttcc
    47941 tgaagggcaa aaaccgctat ctgagatgat ggcagtgacc tggttcattg acgaacaatt
    48001 cgcctacgat cacatgatcg aagcccgcga cttctactta atcaatctgg cagagtaaca
    48061 cacctatcct tgttccacgg cacggacctt cgcttacagg tccgtgcctg gaacctgggc
    48121 gtagaaagaa aattcaaact ggaagaacag taccctttcc tgaactcggg aaggtgacat
    48181 aaccacaccc tggagagtgt tatggcgctg agaaccatgc tttttgatgt cggcggaacg
    48241 cttgaaacct acagttgtag cagagaatta cgaattaaaa ataccgccgt cttcagaaat
    48301 tttttgaaag agaatggcat tattctcccg tatagcgatg agcaactata cgaagtgatc
    48361 tcgagcggta taagcgccta ccgcaagtgg agcatcgcgt cgatgattga gctcagcacg
    48421 gaggaaatct ggtcacaata cgttttcagg ggacacccgg ttgaccagga aaagcttaat
    48481 cggatttctg agcaattatc ctgtctttat gaactgaatt attacgtgcg tgaaatgcgg
    48541 ccggaagttc ccgcggtttt ggctgaaatt aaaaagatgg gcttccgaat cggctgcatc
    48601 agcaacacgc aaagcctcac ccaggttcca gcaacgctgg aaaaatacgg catcagccaa
    48661 tattttgacc cggttgtgct ttcctcaaag tatggcaggc gtaaacctga ccccgcgatt
    48721 ttttatcatg cggcgcggtt ggcgaactcc cctaccggcg cctgtgggta cgtgggggat
    48781 aaaatcaatc gagatgtact gggcgcaaaa aaagcaggtt tcaaactcgc catccaggtg
    48841 cggcatgaat tcgatgacgg tgaagtcgat gaaggagcag agccagacgc aattattgag
    48901 aacatcgccg acctgcttcc attgttagaa gcctcgattc gtgaaaaccg ttctatcagc
    48961 cgggaagatg agcgctcgcg gataaaggcg gtattttttg atgccggaga tattttatac
    49021 taccgcccac agcgtgataa atacttaaat gaattcttat ctggtcatca actaaaccct
    49081 gtgccaggtt ttgaggatga gcgtaaaagg attaaggatc tggctttttc tggtcagatg
    49141 aggcggcacg actattaccg tgaactgatc cagctatatg gtattaccga tgagcaggct
    49201 attgaacagg gcatggaagc cctcagccgt gatgacgaca cagtggccat ctttgatggc
    49261 gttcctgaaa cggttcacac gctgaaagaa agagggttta ttctgggaat catcaccgat
    49321 acagccatgc cgataacccg caaactcaac tggtttgaaa aacacggttt cggccgggtt
    49381 tgggacgtgg tgatcagctc taaggaaatg ggggtgcgca aaccctcccc cagtatgtat
    49441 gaagaagccc tccaacaaac cggtatccgc tctcaagaag cggtatttgt ggggcataaa
    49501 acccacgaac ttgaaggcgc cagggctgta gggttaaaga ccatcgcctt taattatgac
    49561 ccagatgcgc cggctgatta ttacattgaa cattttcaag acctgctgaa ggtgccttta
    49621 ttggcgaact aatccataac gaatgaataa ggaaacgaat gcaaagaaaa gtaaaagcga
    49681 tcttttttga tatcggcgga acattacgcg tgacacaccc tgaagagggc agggatatgt
    49741 caaagattca ggctttaatg agcctgatcg gagaaaagtc agaccctttg gactttgtcg
    49801 ctcgtgtgca taaaggtgaa aaagcctacc gacgctggtg taaaccaaat tacattgaac
    49861 tgtcggaggc agaattatgg acacggtttt tattgccaga atacccggcg ccatttatcc
    49921 gtgaaaacgc tattaccatc aaccaattct ggcgtgaatc aaagccaaaa tacgtgcttc
    49981 cggatatggt ggagaccatg cgggagctct ccaagcgggg ctacaggctt gggcttatca
    50041 gcaacaccac ctcctctgta gagggttacc agctgctgga agaaaccggg ttgaccaggc
    50101 ttttcagcag cgtaattctt tcagcggagt tcggcaggcg aaaacctcac ccttccctct
    50161 tcatcgaagc cgcgcggagg gcaggcgtga gagcggatga atgcgcctat gtgggtgacc
    50221 gcccttcgcg cgaccttatt ggagcgcgcc aatccgccta cgccgaagtg gtcatcatca
    50281 atagcgcggg ttacagcccg gatgagtatg acccggatga ttatgaccca gagaaagata
    50341 ctcatctcac cttgcgccct gatcattgga tcaaacgcct ttcggaactc ctggagatct
    50401 acaagcccat aaagaccgct gcacaggaga acccacaaaa ggaatcggag tataaacttt
    50461 tcgatgtggc gctctcgacc atgtggcacg ttggtcaagc ggcatctttt aatgaaacct
    50521 ttgagcttgt gcgaacgatc ggcatctcac ggtttgaatt caatcaccag gtcactccac
    50581 agctgctggc aacgtttgac tgcaaccgca attacatcag cacggtgcac gacccctgcc
    50641 cggcgccgcg tgatttaaga gccctgaaaa atgacgacat tttaatctcg agcctgaatg
    50701 aagcgcgccg ggggcaagca gtcgacctgg ttaaacgcac gcttgacctg gcagtttcgc
    50761 tggggtcgcg ctccatggtg ctgcacacgg gctcgttgca gtgtgatcgt gcgcgcgacc
    50821 gccggctgcg ccagatgtat gaggaggggc agcgtggttc tgaggagttc tgccagctaa
    50881 gagaagagat gattgcccac cgcgcgcaat acgcgccagc gcatttagac gcggtcaaga
    50941 ggagtatcgc tgagattatc accttcgcga aaggcagcgg cgtgaacatc ggcgttgaga
    51001 accgctatcg ttattatgac ctgccgcttc caagtgaaat gcaggagatc ttagatttat
    51061 gccaggaatc ctggttcggt tttcagctgg atactgggca cgctcacgcg ctcgaaacct
    51121 tgggtttatg cgaaaaagga gtgtggctgc agcgattcca ccaccgtatc atcggagttc
    51181 acctgcacga tgtggctggg ttaaccgacc accaggtgcc cggcannnnn nnnnnnnnnn
    51241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    51301 nnnnnnnnnn nnnnnnnnnn nnnnngaatt tttagccatg gtcagccggg gagaagcgcg
    51361 ttacaaggat tggcgcggcc tgagtcttca ggcgctacca gttgcagaaa aatgggcgag
    51421 attcttgctc gctgaactgg attatgaatt tgtgtttcag cgaggcgaac ggctgcagca
    51481 gttgtggagc gcgtctaagg gcagtaaatc tatccgcgct tacgctcctc tggttctgca
    51541 cgaacttaaa aaccgcggat acctgctcgg caccatcagc cattccacac ccaggtatct
    51601 ggataattcc tccctggcgg acctgttcaa ggttaaggta cacgccccgc aatttggcat
    51661 gaaaaaaccg cacccctcgc tttttgtgga tgccgcccgg cagtgcgggc tttcgccgcg
    51721 tcaatgcgcc tatgtgggag ataattcatg gcgtgatgtg gtcgggccgc gtgaagcggg
    51781 atacgaaatg gtgatcttct tacgcaatgg cggcggagat ccgggtcctg ctgcggaaat
    51841 gatgcagcct gacctggtca tttctgacct gcgtgaaatt ctggattatc tgcctcagcg
    51901 ggtgggaact gagcccctgc aaccactcac tgaactccag gaggtgttgt acgatgcagc
    51961 gttatcaact atgtggtgga agaaggaaga gctttccccc gaagcgttct ttactcgtgg
    52021 acggagaatc ggttttgccc gttttgagct gaaccaccaa atcccgcctg ctttgctgga
    52081 aaaaatcgat caccagcgct tttctatcgg ttcactgcat gacccctgcc ccgccctggt
    52141 cccggcaaaa acgctggaac aaaccgacgt tcaaatcact tcactggacg agaatttacg
    52201 ccaaaaggga gtggatgtgc tgaagcgcac cattgaacag gcttgccgca tgcgcgccag
    52261 gctggtggtg gtgcacccgg gcaggatcac aggcgatcat tcattggatg accagttgcg
    52321 cagtctgttc agagagggga agaagggcag cctggaatac gagcaattga aacaaagcgt
    52381 catcgccaac cgggcagaga gggcagcgcc gcatttgcaa caatgcctgg aaagcctgcg
    52441 cgaaatcacc tgtttcgctg acggcagcgg agtgatgatt gggctggaga atcgttttca
    52501 ttattatgaa ttgcctgttt tcgaagagat gggtaaaatc atggacgaat ttacgcagcn
    52561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    52621 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna aatctgaatt actccaggag
    52681 gatcagtgtt aaaaatcttt aagaaacgcc gtaacgtttt tattcacctg atgcacgagc
    52741 aggccaccct cacgctgcag gggatggacc tgctgaagga ttacctctct tacccggcca
    52801 aacaactggc agacgagctg acaatgaaag aaaaagaggc ggatgaatgc cggcgtattc
    52861 ttattgatga attgaaccgc acatttatca ccccctttga tcgagaagat attttttcgc
    52921 tttcgcgtgc cattgatgac atcatcgatt atgcatacag taccatgaca gaaattgtca
    52981 tcctcaaggt tgaccccacg gaattcatgg tgcgcatttg ctccttgctg cgtgatgccg
    53041 cgtacgagct gatgatggct gtggaacaat tacaggatca cccggcagtg gcagctgaac
    53101 acgcgcagcg cgccaaagcg ctcgaaaacc gtgtcgaaac ggtgtaccgt gaagcgttag
    53161 cagatatgtt ccagaaggcg gacgatgtta aagggctggt aaggataatg aagcttcgcg
    53221 aagtttaccg ccaccttagc aacgcagctg accgcggtga cgaagccgca aatgtaatcg
    53281 cagacatcat cgttaagacg acataaaaaa acatgtcctt cacccttata cttttagttg
    53341 gcttagccgt aatttttaat tttcttaatg gagttcacga ctctgcaaac atagtggcaa
    53401 cagccatctc atcgcgggcc atggctccac ggacggcgct tattgtggtg gcgatcgcag
    53461 aattctgcgg ccctttcatt ttcggcgttg cggttgccaa tacgatcggg aacggtgttg
    53521 tcgcatccga tcagatcaat tcgcaggtga tcattgcggc attaatcagc gcgatcgtgt
    53581 ggaatatctt aacctgggta ctgggtatcc ccagcagctc catgcacgcg ttaattggcg
    53641 gcttaatcgg cgctgtggtg gcgggcgcag gctttatggc gatcaaactg gatggtgtga
    53701 tcaaggtatt gatctcgcta ttcgcctctc cgatcattgg tttttttgcg gggattttga
    53761 tcgccaacct gatttttctc ctttcgtgga agagttcccc aaaaatcaat ggggttttca
    53821 aaaagctgca aatcatcacc tcgttggggt tggcgttgag ccatggcacc aatgacgcgc
    53881 agaaaaccat gggcatcatc actttaggtt tgctcatcag cggcatgatc tcaagttttc
    53941 acgtgccgtt ttgggtgatt tttgtttgcg ccctctcgat ggcaataggc atctccgtgg
    54001 gaggatggca attgatccga accctggggt ccaggttcta taagatacgc cctgtgcacg
    54061 ggttcgcctc tcagtttgct tctaccgcgg tcatcctctc cgcctctcta ttgggcgccc
    54121 cggtgagcac cacccaggtg gtcagcacag ccattatggg ggtgggcgcc gcggaacggg
    54181 tgagcaaggt gcgttgggga gtggccatca atatcggttt tgcctggttg tataccatcc
    54241 ctttgaccgc gctcctggcg gctggtattt atgcttttat gaaatttatc ctccccatga
    54301 tcacctaact ttattaagag gtacagatga cagacacaag tacaaagtac gatgcgattt
    54361 ttgtgggcaa ctacacgaaa gataccatta tcacgcctga aggtacgcgt tacgcggatg
    54421 gcggcgggat gaactatgcc gcgcacgctg ccagcgccct ggggctgaag gctgcggtgg
    54481 taacccgcct ggcaaaagaa gatgagcatg tggtcaacg
//
LOCUS       DAOTLW010000007        52651 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_759_length_52651_cov_11.194159, whole genome shotgun sequence.
ACCESSION   DAOTLW010000007 DAOTLW010000000
VERSION     DAOTLW010000007.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 52651)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 52651)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..52651
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_759_length_52651_cov_11.194159"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(52..1773)
                     /locus_tag="P5516_02095"
     CDS             complement(52..1773)
                     /locus_tag="P5516_02095"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT91262.1"
                     /translation="MDTPKLKILLLGPPEVTLEDRPVLIKRRLNRALLFYLAAQNNPV
                     TREEICNLFWPDAPEEIARKNLREALSRLRTSIGLTDLIFSEGEQLFLNPERVWVDSR
                     EMDRLITPLMSSSEMHRGSALPEWMVAQLKEGFALCRANRFMQGTTLNSAAGFENWME
                     LTNQAYRYSRIKIVDRLIDHYISGGNLEEALIWLGKGIDINPFDEDWNYLALICLRDT
                     GRIQEMIDYITYMEKLYQQQEEELPARFTAFKTDAIARKEIENHPPTDWPEQEEGEPP
                     FICREHELELLNQALRKRGVLLVQGEEGIGKTRLLKQFYITQPYSPALLYCRSLPLGR
                     NTPFMALTQPLRDQIQEVVWQALEPSDRQLLSHFYYHVLHGTEGFTLPVSAGEPQRVL
                     ENVFYAFLNLLKITASNRPLLFVLDDAMWMDQASISLISFLIEHKFFDQYGLLVILVS
                     PEGDNPELDALLQRRSRSRKLETIRLAPLQNREIGLFVQRIFGKTADEDLVKKMHCQT
                     GGNPFYLIECLRAMQQKNISSDQLSDFGACSPPESIVRLVRGKMNLLEKASAAALSAA
                     AVLGRTF"
     assembly_gap    1899..1998
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1999..3676)
                     /gene="recN"
                     /locus_tag="P5516_02100"
     CDS             complement(<1999..3676)
                     /gene="recN"
                     /locus_tag="P5516_02100"
                     /inference="COORDINATES: protein motif:HMM:TIGR00634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HRT91263.1"
                     /translation="MLTELRIENFAIIHKLELTLDNGLLIFTGETGAGKSIILDAIMA
                     LVGGKVDGSLVRQGEDRAMLEALFDIPAACREQVSAILEREGLFEGEPFLSLGREIRA
                     EGRSVSRVNGRSVNVSLLKELGSLLVDIHGQSEHLSLLNVRSHLDLLDRFAGCAAALE
                     AYQTEFHALQQIRKELKALRELEREAARRQELLAFQAQEIEAACLQPGEEQELEQERN
                     RLANAEVLTTNTQEVLNILDEGSPETSSASELLGQASKLLAALARVDSQQVGLVDQLE
                     EASTTLTELSRDLRAYLDGIEFNPRRLEQVEERLELIHQLQRKYGGTVEAVNEFGLSA
                     RRELEKVQHAGERIEELEQEENAAILKTLSAGVNLSRLRRQAAQRMGQEVESELNDLS
                     LAGARFSVWFNELPELDGLMPQDALKVDLKESGLDEVEFLIAPNPGEGLKPLVKIASG
                     GETSRLMLALKNVLAQADHIPTLIFDEIDQGIGGRVGSVVGEKMWRLARRHQVMCVTH
                     LPQLAAFGDEHYKVSKSVIDQRTLTGVKRLGEDERIEELALMNGSISLSSL"
     assembly_gap    3705..3804
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(3842..4375)
                     /locus_tag="P5516_02105"
     CDS             complement(3842..4375)
                     /locus_tag="P5516_02105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91264.1"
                     /translation="MAEEATSESMHCYVHPQRETLLRCNRCEKPICLSCAVLTPTGYR
                     CKECVKGQQRIFETARWWDYPLTFLTAGVLSFLGSLIAGLFGFFTILLAPVAGMIIAE
                     AVRFVIRRRRSKPIPVIAVAATLIGGLFVPVANTLFALLNFYIHTAGGLLNLLWPAVY
                     AILAASTVFYRLKGIRI"
     gene            complement(4375..5274)
                     /locus_tag="P5516_02110"
     CDS             complement(4375..5274)
                     /locus_tag="P5516_02110"
                     /inference="COORDINATES: protein motif:HMM:NF039482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HRT91265.1"
                     /translation="MRPIKLRYNKETMSTPSVHRIAIVVRPGSTDTLSEGAVIQQWLK
                     SHGVSSTVTTTDNQHLRRKISQGEYDLLIALGGDGTMLRAGHLAAPQGLPILGINLGK
                     FGFLMQLSREDWADTLPRLLAGDYKVENRMMLQAQQWRGDERLRTSQVINEVVVARGQ
                     VVRPIRIHAEVDGYPLASYVADGLIASTPTGSTAYALAVGGPIMPPELRNILIMPVAP
                     HLSMDRAVILPEGASVVITVDSDHEAVMSVDGHEPALISNQDRVTVTASDLSVKFVVF
                     QEPGYFYRNLSNYMEQNPSIGDF"
     gene            complement(5452..6093)
                     /locus_tag="P5516_02115"
     CDS             complement(5452..6093)
                     /locus_tag="P5516_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase"
                     /protein_id="HRT91266.1"
                     /translation="MNEFNEIVESTRKVFDERTKVRDAALVQARQLTRVSALTIRAIH
                     RGDKDEAHGLLNEARQLVLTLQRELADYPDLYHAGYTQDAIKEFAEASLTCALIENRR
                     LPRPEELEIEHATYVNGMAEAIGELRRRCLDILRQGYSVEAERLLNTMDEIYNFLVTL
                     DYPDAITNGLRRQTDLVRGIIERTRADLTLSLREQHLQEALQRFSEKYPGLAE"
     gene            complement(6097..6432)
                     /locus_tag="P5516_02120"
     CDS             complement(6097..6432)
                     /locus_tag="P5516_02120"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRT91267.1"
                     /translation="MSNRPQNPPLGLPPMELPDELEPVYSNLARISHTPSELAVDFSR
                     ILPGQKRLTVLVRVLLSPVGAKLLLRALAENIARYEAAFGEIYLPGDSGLAEDLFRSV
                     HPPEPPKSG"
     gene            complement(6442..6855)
                     /gene="ssb"
                     /locus_tag="P5516_02125"
     CDS             complement(6442..6855)
                     /gene="ssb"
                     /locus_tag="P5516_02125"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRT91268.1"
                     /translation="MYQKLVIVGRLGRDPEMRYTPTGQAVTSFSVATDRQYSDQSGKP
                     VKETVWFKVTAWGKLAETCNTYLQKGKLVLIEGRLTVDTKTGGPRVWTGQDGQARASY
                     EVTAQTVKFLSQRQEGGEPGQEEELDIAPSEEIPF"
     gene            6953..7837
                     /locus_tag="P5516_02130"
     CDS             6953..7837
                     /locus_tag="P5516_02130"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT91269.1"
                     /translation="MTAQPRAARFGAGSKNQPGNNVIILPVNAIKPRSILIFLFILLA
                     AAVLVITSALISTPAQAQVLYFTPTADDSGAIYYIVKQGDTCDSIALVNNIPVDTLRN
                     QNSLDFDQCRFLQIGQKLLLGTVPTAVITPGPSPTPTSILPTPEPVKGFGTLCIYLYN
                     DINGNAMAEEEETMNTGIAGGQVSITHLGGEYSNVGTTLSTGEALCFNDIPEGEYSIS
                     IAIPDGYNPTSSQNVTVLLKAGDTSTVNFSAQVNGLAESPRGNGDAGGSVLLAVIGGL
                     ILLAGVGVGLYARYILRK"
     gene            complement(7890..8528)
                     /locus_tag="P5516_02135"
     CDS             complement(7890..8528)
                     /locus_tag="P5516_02135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HRT91270.1"
                     /translation="MDSIFRRKTEEEQMKAAGRMPPGQFLTQKFPVLHYGAVPGFDPV
                     NWDFCVWGEVEQTLRLTWAEFSQLPRTTLIMDIHCVTRWSKFDTLWEGVSIRTLIENR
                     LIRLKPQARFVIQHAEYGFSANLPIEIALADNFLLATHYDGAPLTPDHGYPLRGVVGA
                     IPQRPDLKTPYFWKGAKWLRGLEFSAVDRLGFWEEAGYHNEADIWKEQRIAE"
     gene            8665..10377
                     /locus_tag="P5516_02140"
     CDS             8665..10377
                     /locus_tag="P5516_02140"
                     /inference="COORDINATES: protein motif:HMM:NF024583.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRT91271.1"
                     /translation="MLIYAQRQDPFETRLLKLHRDAAELVGNLSYDEVLEKIASIALE
                     RSGAGYAAVATLDEHGEIERFVPAGMLRTQIEKIPHPPQGFGLIRAMMNTRSAMRIPA
                     ISKDQRHSGFPPEHPKMSSFLGVPIFQGQRQIGQIYMTDKVITGEFSEEDQRLMELLA
                     AYAGVAIVNARLFRDYAMRDRALTRQNENLSLLYQLASTLSTSTEVNEILEQGMMQLM
                     DYLRLEVGEIFIKNEDSRRLNLLIHHGEAVDSLWKRAQFSIGESTVGRAAKGDVPIVL
                     NLTEHEYADLNPLTKARGLHQMAVIPMKGRRGVVGVLCVATCHPQPLNEPDVQFAQAI
                     AAWMATAIENIRLNITGRRLAILEERDRIGMDLHDGIIQSIYAVGLTLEHARLLMKDN
                     PDTATSRIQQAVNDLNSTIRDIRAYILDLRPRQLKNENLMEGINRLVQEFRANTLVDV
                     TLNGPKDGVATLPEAQALALFHICQEALANIAKHAKAKRAYVSVWTTSERVLLEVHDD
                     GLGFEQNKTKLSIGHGLSNMETRAINAGGEVDISSEPGGGTTVLAWVPFPDPDFSSND
                     RVIE"
     gene            10426..11415
                     /locus_tag="P5516_02145"
     CDS             10426..11415
                     /locus_tag="P5516_02145"
                     /inference="COORDINATES: protein motif:HMM:NF024575.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta' C-terminal
                     domain-containing protein"
                     /protein_id="HRT91272.1"
                     /translation="MSWNLLGHTWAENLLRTHIANNEARHAYLFTGAPGVGRRSLALA
                     FASALNCTQPQQPGDYCENCRICRHTQRMQLPDLSVVAPESEGGSIKVAQVRELQRAL
                     SLTPYEARYRVALLLNFQLANASAQNALLKTLEEAPEKVILLLTADAADSLLPTIVSR
                     CEVLRLRPLAVEALEDALLNRWQLAPQQARLLAHLSSGRPGMALQMAQDQHLLEKRAA
                     WVQELLRLLPANRYERFSACEALARNRDQLRLMLQVWLSFARDLLLTASGSSEKIVNQ
                     DQLAEIERIAGGLPTRRPLEMVNTLITSLENLEANANLRLLLDNTLLKIPQIN"
     gene            complement(11408..12163)
                     /locus_tag="P5516_02150"
     CDS             complement(11408..12163)
                     /locus_tag="P5516_02150"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91273.1"
                     /translation="MSNGFLEVRQLGMTFRGAEGGLEALREISFDLGSEEFLCVLGPS
                     GSGKSTLLRLIAGLLKPTSGWIRFGGADGAPRVGLVFQHANLMPWRTVRQNIALPLEL
                     LGMAEEESASRVAEMMRLVGLEEFGSNWPSELSGGMAQRVALARAFIQEPDLLLLDEP
                     FGALDALTRERMGSELLRIWQGQRVPVVMVTHSISEALLLSDRVLLFTPRPAEISKDI
                     EVTFPRPRKEELRYSADFQRLERSLRGSLQYIS"
     gene            complement(12173..13183)
                     /locus_tag="P5516_02155"
     CDS             complement(12173..13183)
                     /locus_tag="P5516_02155"
                     /inference="COORDINATES: protein motif:HMM:NF020650.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT91274.1"
                     /translation="MKKTSQTFFLLLFIGVILIALFAVTRVIAPAVEPTSTSAPLRKV
                     RLPVGYIPNVQFAPIYVAIEKGYFRDAGLDVAIDYSMENDNTVLTATGDLQFAVVSGE
                     QVLLARAQQMPVVYVMAWYQQYPVGIAAKKSAKINTVEDLRGRKIGLPGLYGASYIGA
                     IALLDSAGLKEADVKLDSIGFTQVESLIADRDEAVVIYVANEPVQLERLGEEITLLRV
                     ADAVDLVANGIITNEMTLQKDPELVRSMVTGMIKGIQYTIDHPEEAFEISKKYVENLA
                     SADQEMQMQVLLNSIALWRDDRLGYSQPQAWENMQRILLKMGLLKDELDLSKVYDNQF
                     IP"
     gene            complement(13206..14039)
                     /locus_tag="P5516_02160"
     CDS             complement(13206..14039)
                     /locus_tag="P5516_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91275.1"
                     /translation="MRTNDDLKTPMGDTLGGSQPALEFLARHNGWLAGISLLVALGVW
                     ALIIDWGKPPVFLLPPPGAVWNKFLQVAGDGTLLFHAGVTLSEVLLGLLAGSLVAMAL
                     GYLLAKSRLLEKIISPYLVASQAIPTVAIAPLLIIWFGQGIFSKVLICALTLFFPVLV
                     NTIIGFRAVPANLYDLMRSMRATHAQTLRYLELPAALPVLFGGLRIGAALSVIGAVVG
                     EFVGADRGLGFLINVGRGQFDTALVFVSLFTLILMAMSLYGLAMLAERRATRWRVADR
                     G"
     regulatory      complement(14103..14237)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(14639..16297)
                     /locus_tag="P5516_02165"
     CDS             complement(14639..16297)
                     /locus_tag="P5516_02165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91276.1"
                     /translation="MTNNFRKKSILIVVLAVFLMACSLTGLLPGRDKDTASSGEYRSE
                     VGGYSFKPIEGYELIEFYGITQMMAPGGNDETGPMIAMMGAKQEEKLDNQKLLGNMKE
                     SLPDAKFSKPKKVKVDGKAGLDIKLEDYEDEDVMGRLVIVMVEPTQHFVLLGVAPKAE
                     WKALNKNYEKVLKSIKFFEPKEMSFDEDIYDDGYIVSPDDFDFEPGQFSTYDEDGQLH
                     QWAVSAVASSQYGDEGWSAKQAVGEPDVEDCQDDIFAWASSSSNTVEWIELTYETPVI
                     PFEVTVVQSYNPSQVVDITVIAEDGTEFSVYEGSPETTQYCPAYLTVSIETTTEIYVN
                     KVRVTIDQSELGLGWNEIDAVELVGMPRLGSYGPTDPTPFFAGGMDFAGDVPEGKGFL
                     TTGPYRKYLSVPINEVVALEPIIGPLKSSSGGLKPRADHKDTYVFDIGGDMKAYVSIT
                     TSGLVYKKTIGPVNQVDMGVEFDSDTYDQLAQLQKDMNYQLPYTLVAQILGSPGLIIW
                     DQIQDDGSRRTEYYWFNSKGDAMWVAAYNGLITGYAGISFMPKN"
     gene            16534..16633
                     /gene="ffs"
                     /locus_tag="P5516_02170"
     ncRNA           16534..16633
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="P5516_02170"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            16659..17558
                     /locus_tag="P5516_02175"
     CDS             16659..17558
                     /locus_tag="P5516_02175"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT91277.1"
                     /translation="MQTPPVCNYEGSDYQTSFWEKGGRDYEDGCEAVALKRLLPAAGS
                     RLLELGAGAGRNTSRYSGYAQVTLLDFSRTQLEQAKARLGTGERYRYIAADIYRLPFV
                     KGLFDGATMIRTLHHMAEPALALAQVRRVLEPEATFILEYANKRNLKSILRYLLGRQK
                     WSPYTREAVEYIPLNFDFHPAAIRDWMDGAGFILERALTVSHFRVSFLKKTLPVRLLV
                     AVDALLQPTGGLFQLTPSVFLRARALKTGVKAPQGAFFACPACAAPLDDTPPLIHCPA
                     CEKQYPVIDDIYDFRLNPPAEGD"
     gene            17562..19790
                     /locus_tag="P5516_02180"
     CDS             17562..19790
                     /locus_tag="P5516_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HRT91278.1"
                     /translation="MPDALLDDLNPQQRRAVTAGDGAVLVLAGPGSGKTRVLTRRLAY
                     LVERRGVPACDVIAVTFTNKAAREMENRVLNLVQQNLDGIWLGTFHAMCARLLRREVN
                     YLPYRSNFVIFDADDQESLVKRAIRELNIDDKTHRPAGVHAAISAAKNDLLLPEQMPL
                     RTYRDEVVARVYKRYQEMLVANNALDFDDLLLQAARLLEQNPGVAERYARRFQHVLVD
                     EFQDTNLAQYTLLQQICAYHKNIFVVGDEDQSIYRWRGADYRNVLRFQQDFKDCATIL
                     LEQNYRSTQNVLDAARQVIDRNGNRTPKHLFTERGTGSKLVQFTAVDEVAEAAFVVDT
                     IQQYLSSMRATASDFAVMYRTNAQSRLVEEALIRARLPYRLVGALRFYGRREIKDLIA
                     FLRLAENPTDEISLARVIAVPPRGIGQKTIEMLQSAAFQANISSGEVLLDLGSKGDSS
                     PFWAIIGRSASLLADFGALLADWHSLKGRVSLVTLFDRIISDTGYEPYINDQSDEGND
                     RWENIQELRRLAYDYDEKGLTEFLQNLALVSDQDTLPPEADQPATARQKAVTLLTLHA
                     AKGLEFNQVFITGLDEGLLPHSRSRDDPEEMAEERRLFYVGMTRARDQLYLLRSERRS
                     SFGNWEYSEPSRFLADISDELLTYQGKRGGLPRAAQFEELRWTTTGLPSTYSRRQRKP
                     ELPVETRFKPGMRVRTAAWGEGLVLESRVDSDGEETVDVHFESVGFKRLLASLAKLEI
                     IP"
     gene            19889..21397
                     /locus_tag="P5516_02185"
     CDS             19889..21397
                     /locus_tag="P5516_02185"
                     /inference="COORDINATES: protein motif:HMM:NF014683.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015129 - lactate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015727 - lactate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate permease"
                     /protein_id="HRT91279.1"
                     /translation="MSPIVAALPILLVLVFMLFLRRGSHEAGFGGWLVGIAVAFLFFG
                     LNWNVLWVSQLKALLLSLNVLTVLWPALLMYHIVEMSGGVRAIAYALQEMIPEPGWLL
                     LVLAWMLSALVENLAGFGVPIAVIAPMLILLGVIPVRAVAAAAIGHSWTVSLGGMALA
                     FRTMTDITAMNESEVFPFAAVLLGVTVLLTGLGSAYVLKQLHYWKRILILSLVVALTQ
                     YFIGITGVIPLSSFSAAIVGIGGGLLLTRRPPGQKIRASTSPQLRGGLFSYGTLFGSI
                     FIISIIKPLNRWLAGARVSSWFPQVVTNQGFTTAAGPGFVFKPFTHPGVWILFSALVA
                     ALVLPRLTRSGTAHMKESLTATWRSALPASLGTLFMIGLSSLMEHSGMTQLIAQGLSQ
                     ALSSYYPLVAPLVGTLGAFATGSNTNSNILFGQMQKGVAALLGISPLVLLAAQTTGGA
                     LGSMISPAKLAVGGSTNAMKGKEGAALRLTLPIGLASTLFIGLLAWILLKVL"
     gene            complement(21522..22559)
                     /locus_tag="P5516_02190"
     CDS             complement(21522..22559)
                     /locus_tag="P5516_02190"
                     /inference="COORDINATES: protein motif:HMM:NF022077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRT91280.1"
                     /translation="MITEKQVYDALSGVMDPELGRSLVELGMIRNVTISRGGVVAFTL
                     AFTIAGCPLKQKIERDAREAVMKLPGVKQVNITQSVMSEEERKALFPPGATELPQLNK
                     FNKIDRVIAVMSGKGGVGKSSITALLACELARQGKKIGILDADITGPSIPRLFGLPPG
                     GLRGSEQGILPAISGLGIRVMSSNLLVPTEEAAVIWRGPLIAGSIQQFWRDVLWGRLD
                     VLLVDLPPGTSDAALTVMQTLPVNGVVLVSTPQQLAGMVVRKADSMLKQLNIPVLGVV
                     ENMSYYPCPDGGGNHEIFGPSHVEELAAAVGAQRWLKLPLDPRVALLGDTGRIEEYRA
                     ENLQEILTLFS"
     gene            22631..23233
                     /locus_tag="P5516_02195"
     CDS             22631..23233
                     /locus_tag="P5516_02195"
                     /inference="COORDINATES: protein motif:HMM:NF022112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HRT91281.1"
                     /translation="MKRSLFLSLLFVVAAVLMACSASVDLFGNKVKPTLNIPPTQPPA
                     IATSSTPLQPTVIIALPPPPSLETSITVYFMDEDRFINGTEPYEIAVSRTTASVNLPL
                     AVLQAYFAGPTVDEYNQGLRMLGSGFSYVRDLTVENGIARVYLGGECANNGAAYSVAA
                     LIFKNLQQFPEITAIKIYDENNSTLDPDSAHSSLPYCLEP"
     gene            23324..24025
                     /locus_tag="P5516_02200"
     CDS             23324..24025
                     /locus_tag="P5516_02200"
                     /inference="COORDINATES: protein motif:HMM:NF024863.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HRT91282.1"
                     /translation="MTITILSVLFAVTLVFLALSRMAVERIETHHYLQRADFFQRYPV
                     QQGDIVFLGDSITDGGCWEELFPGVPLKNRGINADNTRGVLERLDDILRPQPRAIFLL
                     IGTNDLPWYTYLNDRAILHTYNQILDRCRALAPDAVVHVQSLLPRQRIYAARILRINA
                     HLETMASRHGYPFINLFPAFADRQGALRADLTNDHLHLMAEGYALWVSILKPYIEQLH
                     NENVNSALPSPGFNP"
     gene            24205..24909
                     /locus_tag="P5516_02205"
     CDS             24205..24909
                     /locus_tag="P5516_02205"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT91283.1"
                     /translation="MSHFTQKSLFWISKPVVKTYTNAMLKMDVHQKTPFVSGAKIIAP
                     NHPSTTDPFFVAAMLGQQSYIMIKDVLFNVPVLGEYLKRSGHIKVAAGQGQEAIDQAL
                     AHLAVGHTIMIFPEGKVSPVKGGFNQVRSGVARLAIASGAPVYPVGIHLLKERIHAIS
                     SRVKGRDEMGYWYLRGPYNISVGKPMRFNGDVDDHEFVRETARLVMLKIMRLAYESEQ
                     RWYRKTRAVVGSLETA"
     gene            25025..26062
                     /locus_tag="P5516_02210"
     CDS             25025..26062
                     /locus_tag="P5516_02210"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 domain-containing protein"
                     /protein_id="HRT91284.1"
                     /translation="MKPQQILAGPGMLLDGQGNLIQAGWATQPVLDANLENVHFYAFR
                     PLQKLRLKLWDYYAITTPTHFFSFTVSDIGYLGMVFAYVIDFKTGAFKEETLALPFGG
                     GVKLPRNSTEGVTEYSKKDLYLRFRAEGEVRQLSIRWPGFGGSALNAEVALTCPADHE
                     SMNIVIPIPGKRFYYNRKVNCMPAEGWVEYQGTRHLIEPTTCLGSLDWGRGVWEYSSF
                     WVWASASGFLPDRRRIGLNMGCGFGDTSAATENCFILEGKVHKLSELKFTYDNRNFKA
                     PWTMSSPDGRLELVFTPFFERVAKIDALVLKSEVHQLIGRYNGKVITDTGEKLEIVDL
                     IGWAEEHNAKW"
     gene            26353..27228
                     /locus_tag="P5516_02215"
     CDS             26353..27228
                     /locus_tag="P5516_02215"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91285.1"
                     /translation="MKKLTIIMPVYNEEESLPLIMNNWLELCAASHCRLVVVNDGSTD
                     GSRRILDNLQSQHDFTVIHHKVNKGYGGAIKTGFRAVKTEYLVTIDGDGQHLPASVIH
                     MYQKMIEEDCDLLIGARENETKFTARGLGKWIIRSISKLLLPNKIRDLNSGLKMYRAE
                     LAQRYIKVCPDSMAFSDIIALTFIAEKCKVMETPIQISPRAGGKSKINLGSAVDTVIE
                     IINIVVFFNPLRVFLPLSAFFLVAGLGWGIPIVLKGRGVSTGSLLAFTISGIILLLGL
                     IAEQLSQIRRILIEE"
     gene            complement(27299..29014)
                     /locus_tag="P5516_02220"
     CDS             complement(27299..29014)
                     /locus_tag="P5516_02220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91286.1"
                     /translation="MPFTSGIKKCRLNEVFLLAEIIISAAVFILLGIFLAKYAGGPIY
                     SDEVMYINHGLLNVKDPFIINRYTHIFFQKLFVEIADRPLEGVKAYWGFLVAGSAALT
                     YVISRLTTKTSNILHAGISTLLYLTIPLLSVYSGNTSVDLTAGIFLGLTVVLYILCFR
                     QPGKPVYLLSLMGLAFFLAFRSKETTLASAVLLIGFLFDGEGGINVRLCLKRLGYFMI
                     GLLAGVVLFMLLNGLLLKDAWFGLRLSEYFQFKKTYIDRNIGEESKAVANWFTGFFLE
                     STPYIFLAYLFSGIKLLKKQPPSMRLPWLIPLKLIVILSVIIGFSSWGVIGRHVFPIL
                     PLVCAFAPQLIVFDFPRNKKEFSWLAFSMLAAALLFIAGRKILVVVSQAGPYNYPDFL
                     ASTVYPITLIALIILVFLVRHYSNKTVFAPLLVFVIFIAYPLYDNTLNYFFEQTNVRK
                     MNERLQAIEVFDSANIKEEKIKLLVFEDALSDLNYSSRDVEFIDLFNMYYDERLTWSD
                     VRVVNFDTFKPQTIVEWQPTFILMSADEYSSSEASVIIQRYIESDYEMIDSGNIRFAL
                     FNRLK"
     gene            complement(29215..29490)
                     /locus_tag="P5516_02225"
     CDS             complement(29215..29490)
                     /locus_tag="P5516_02225"
                     /inference="COORDINATES: protein motif:HMM:NF012434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91287.1"
                     /translation="MSSNLCPFPFLIPDFLCNAGGVTVSYFEGVQNDMNYYWTKEEVL
                     EKLDTKMTIAFAGVLDMSQKSKVYTRDAAYMVAIDRVVKAMQLRGWV"
     gene            29480..29734
                     /locus_tag="P5516_02230"
     CDS             29480..29734
                     /locus_tag="P5516_02230"
                     /inference="COORDINATES: protein motif:HMM:NF022427.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2804 family protein"
                     /protein_id="HRT91288.1"
                     /translation="MDDIIFTCDDQNLKSLYTMTLPDGRLALVFTPFYERVEKSDTLV
                     LKSGSQQMFGRCKVKRLTISGEKLDIIDLIGWVEEYNAKW"
     gene            complement(29745..31781)
                     /locus_tag="P5516_02235"
     CDS             complement(29745..31781)
                     /locus_tag="P5516_02235"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRT91289.1"
                     /translation="MLIFSALVYLFLALLLSLRIFKSVSFSHSVIIFFLSSFTLNVLI
                     AEVLSLLGMLNSRWMYLLLQLLFCLALAMLIWDPPKKVFKNPIPKLKFEFRRLYGWEI
                     FLVALVSGILLLCLYIGALAPINNSDSLHTHLPRIYYWLQHGSLASWNAVTVTQLNYP
                     VNISIQGASLFLQGRSEKLFYLVPWLALLAVVLLIYEIAMLLGASRRAALFAGLLGLS
                     FPVVLLQTFSYQGDVLVATLVLAAVWFLLLYVRQQESFFLYLSFVPLAAALGSKQTAF
                     LFLPFFLLALLILFIKNHTRVEVILGAAGVFLAFFMLLASYKFIQNAVERDHLESSMF
                     AGYRYRLPFSKPGDGLHYATNSGRYLYQAVSLDGLTGRLKLAAQEARAHAFRAKSEWL
                     NLDLEVRDYISDGDDEYFSFAEAPALNEDSAWFGPLSFVLFPIAVVLVLTGKCKLCKR
                     YQWLSLVFMLTVFLMVAVLITGWSPTNGRYLILPMLVFTPLFAVLIPQKRAWSGLVTL
                     ALSIASFYLAISTLLINDARPLVTQQSLYSFQAKKVEKWGDSGFIGGIAAKHIANRVI
                     EDLVLTSPDRKDIRQQSYYENLFHQSTSAIADVEFVNSTVKPHEKLYLFIDKDIIEYA
                     LFGVNKTRDLVPVVSLQQLPDGALVLVDKRRIPSITSGFTLLAENDHFSIYQKP"
     gene            complement(31810..32205)
                     /locus_tag="P5516_02240"
     CDS             complement(31810..32205)
                     /locus_tag="P5516_02240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91290.1"
                     /translation="MLGEIHKENVANASPPLIAVFIPAYVSFFLIASLMLVFLGGIWG
                     TVKWLEPDLSEVFTTTGIVFIAVPSIIQAMQFLIQTITLDTSSVSVEPLVRRMPKKPS
                     MKGPLMMWLHERIERGGVRVPLSPVLHPA"
     gene            complement(32296..33582)
                     /locus_tag="P5516_02245"
     CDS             complement(32296..33582)
                     /locus_tag="P5516_02245"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HRT91291.1"
                     /translation="MATSINAFEMAQKQFDNVAELLKLDPQVREILRWPLREFSFRIP
                     VRMDDGSLRMFQGFRVQHNDARGPNKGGIRFHPAETLDTVRALATWMTWKCAVADIPL
                     GGGKGGVVVDPSTLSTGEKERLVRGWVDVMWRNIGNRQDVPAPDVGTTAQMMGWMMDE
                     YSKLVGEFTPGVITGKPLGGGGSSGRTEATGFGVIINVREAMKHLKLDGAKCTASIQG
                     FGNVAQYASIGFTEMLKGKVICISYYDREDKVSYTVSKADGIDPRFLMTITDQYGTVD
                     KVKAREAGYVIEDGGAWISKDVDVLIPAALEGQINGETVKQISPRVKILAEGANGPTT
                     PEADEVLKQSDIFVIPDFLCNAGGVTVSYFEGVQNDMNYYWTKEEVLEKLDTKMTIAF
                     AGVLDMSQKSKVYTRDAAYMVAIDRVVKAMQLRGWV"
     gene            complement(33811..35307)
                     /gene="gatB"
                     /locus_tag="P5516_02250"
     CDS             complement(33811..35307)
                     /gene="gatB"
                     /locus_tag="P5516_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HRT91292.1"
                     /translation="MDGTGCDKIHYIMQYEAVIGLEVHAELATQTKMFCGCRVVDTTT
                     SRPNSSVCPVCAGMPGTLPVLNRRAVEYGVRVALALECEVNHTSIFARKNYFYPDLPK
                     GYQISQYEQPLAENGRLVIDTSEGERVIRVRRAHLEEDTGKLTHITDDGGDCSLVDLN
                     RAGVPLLEIVSEPDMHSVEEVRAYATGLRAILRALGVNSGNMEKGVMRFEANVSIRPA
                     GSDQLGTRVEIKNLNSFRAMERAIAYELARQASLLENGETVSQETLGWDEPNQRTYVQ
                     RSKEEAHDYRYFPEPDIPPLAVEADWIAQIRQRMPELPRDKTRRLQQQYGLIPADAAI
                     LTSESDIAAYFEDCAAALKQASARSAANWITGELFAHLNSTGAEITHIRVSPQSLAEL
                     LDIIEAGGINQNTAKTVLAEMLASGSSAQQVISEKGLAQVTDTGFISDLVKEVLTTNQ
                     KELASYLAGKETLANWFFGQVMRAAGGKASPAVLKEELEKQLTEAGRG"
     gene            35378..36148
                     /locus_tag="P5516_02255"
     CDS             35378..36148
                     /locus_tag="P5516_02255"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: protein motif:HMM:NF009848.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III pantothenate kinase"
                     /protein_id="HRT91293.1"
                     /translation="MLLCIDIGNTNIKLGLYEGEKLVTHWRIYTDEHKLADEYAVLLL
                     SLFKSEDIKNEAISGCAISSVVPELTIAFRDLVRRHLNLEPVIVGNVKRPVIHINTSN
                     PKEVGPDLIANAVGAVALYGAPCIVIGFGTATTFCAISASGDLEGVVIAPGVITAASS
                     LFQAGAMLPAVDLHKPEKVIGKNTILSLQSGLIYGFAGLVEGLSARIKAELGGDAKVV
                     ATGGLARLMADEVDCIDAVEPELTLLGVRIIYEKNQKG"
     gene            complement(36425..37585)
                     /locus_tag="P5516_02260"
     CDS             complement(36425..37585)
                     /locus_tag="P5516_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRT91294.1"
                     /translation="MTDRYLSRRVAGLKPSGIRKFFDITATMKDVISLGIGEPDFTTP
                     EPILMAGIESLQRGETHYTSNYGLIQLRRALAAHFSTLYNVEYDPEKEIIVTVGGSEA
                     LNLAAIALVNPGDEVIIPTPCFVSYQAAVILAGGVAVEVPSHMESNFDIDPDALRAAI
                     TPRTKAILIGFPNNPTGAVASRENLLEVARIAEEHDLVVISDEIYDRLVYGVKHVCFT
                     ALPGMRERSLLLGGFSKDYAMTGWRIGYAAGPEHLITGLLRVHQYMVMSAPTMSQFAA
                     LEGLLSGEPHVLKMLAEYDRRRKLIVSGLNQIGLPTFEPKGAFYAFPKVSVTGLDDDT
                     FCTRLLHEQRVAMVPGNAFGMGGEGFARCSYATGYNQLEEALERIERFVRKI"
     gene            complement(37582..37818)
                     /locus_tag="P5516_02265"
     CDS             complement(37582..37818)
                     /locus_tag="P5516_02265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91295.1"
                     /translation="MRCRAEYAYPGRPLEVWDGRRWQKVIEVLDEIQTPLDKRFKVVC
                     ENGNEYWLVYDPDRDGWQVTAAGSDGKITQKENQ"
     gene            complement(37827..38840)
                     /locus_tag="P5516_02270"
     CDS             complement(37827..38840)
                     /locus_tag="P5516_02270"
                     /inference="COORDINATES: protein motif:HMM:NF012695.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRT91296.1"
                     /translation="MSQVFKIIIPMAGYGSRMRPLTWSRPKPLLPLAGKTVLDYALEQ
                     FSSLPGVENAEYVFITSPNQGDAIEQYMRHAHPQKKVHFVIQAEMRGQSHALWCAKEY
                     LAGPALIAFSDTLIETDLQFIPFEEMDAVAFVKAVEDPRRFGVIEIGKNGIATRLVEK
                     PQDTSNNQVLVGFYYFKDTAALLRAIEEQMRLGKSLKGEYFLADAINIFIQMGGRMRK
                     ENIDTWLDAGIPSAVLETNRHLLDQGRVATQPDSGIPGATIVPPVFIAPGAKIEACVI
                     GPYVSVGKGCTLKNVVINNSIVDDNTVIENMVIEGSIIGREVVYKGQPARLFVGDHTQ
                     VQS"
     gene            complement(39005..39445)
                     /locus_tag="P5516_02275"
     CDS             complement(39005..39445)
                     /locus_tag="P5516_02275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HRT91297.1"
                     /translation="MTQQNTRVELIERGPGCLVRGLYFLFIGLWLGLIWVVVAWFFNL
                     TIVGLPIGLLMINRIPQVMTLRPQRVQTTVEIREGSPVIRQTTIRQNPFLLRALYFLV
                     IGFWFSLVWMLLAWLLTAVTFGLAFPLTFWMFDQVPAITTLARY"
     gene            complement(39538..41016)
                     /gene="gatA"
                     /locus_tag="P5516_02280"
     CDS             complement(39538..41016)
                     /gene="gatA"
                     /locus_tag="P5516_02280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HRT91298.1"
                     /translation="MNTSLTDLSVIQILDGLKKAEFSCRELVQAYLERIAILEPRVHA
                     FVTLAEEDALSLADKADARYASARHSGSETPPLLGLPIAVKDLITVAGMRCCCGSAIL
                     KDFVPPFTASAVQRLLDAGAIVVGKTNTDEFAMGSSTETSAFGSTFNPWDVTRVPGGS
                     SGGSAAAVAAGMVPAALGSDTGGSIRQPASFCGVCGIKPTYGRVSRYGLVAYGSSLDS
                     MGVFTRNAEDAALLFSLMAGIDPLDSTTVDVPVPVLNLREKERLDGLRVGIPQEYFIE
                     GIQPQVRHCVLAAIDTLRLLGAELVEVSLPHTEYALPVYYLIAPAEASANLARYDSVR
                     FGLREPAATLWEMFKRSRGVGFGAEVKRRIMLGTYALSAGYYDAYYGQAQKVRTLIRR
                     DFDQAFEHVDLIACPVAPSTAFKLGEHAGDPLAMYLEDVFTLPTNLAGIPGLALPVGF
                     DDNHLPIGIQFLGAHFREDVLFRAAHAYQGVTDWHQQVPALD"
     gene            complement(41013..41300)
                     /gene="gatC"
                     /locus_tag="P5516_02285"
     CDS             complement(41013..41300)
                     /gene="gatC"
                     /locus_tag="P5516_02285"
                     /inference="COORDINATES: protein motif:HMM:NF014716.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HRT91299.1"
                     /translation="MKLSLVEVEHIAELARLDLTAEEKEQYRQQLSAILEHVARLQAL
                     DTEGVPPTSSVLPANGRLRVDEPRPGLTVESVLANAPEKMKDQFRVPPVLE"
     gene            41420..41824
                     /locus_tag="P5516_02290"
     CDS             41420..41824
                     /locus_tag="P5516_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4332 domain-containing protein"
                     /protein_id="HRT91300.1"
                     /translation="MPSIIDIEGIGSVYQKKLKAIGVATTDKLLELGATPKGRKELAE
                     KTGTGEALILEWVNHSDLYRIKGVGSEYSDLLEEAGVDTVVELSKRVAKNLYEKMVEV
                     NAAKKLVRKLPVEKQVADWIEQAKKLPRKVSY"
     gene            41905..42435
                     /gene="yjjX"
                     /locus_tag="P5516_02295"
     CDS             41905..42435
                     /gene="yjjX"
                     /locus_tag="P5516_02295"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0046128 - purine ribonucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HRT91301.1"
                     /translation="MKLVYLASENPVKIAAVEEGFRRVFTGEEFSVRSVSLSLNLPAQ
                     PKTDDETLFCAEQRAANARETAPDGDYWVGIEGGTSERGGEMGTFAWVVILDSLRRGI
                     GRSGEFFLPKILADLVRQGMELGDADDLVFARQNSKQKNGAVGLLTADAIDRLELYIP
                     AVIFALIPFKNPLLFP"
     gene            complement(42466..43359)
                     /locus_tag="P5516_02300"
     CDS             complement(42466..43359)
                     /locus_tag="P5516_02300"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRT91302.1"
                     /translation="MPRTATTLISLLTCLFLVSCAAVNSSPIPTVAATDTSTPHPTST
                     PVPQATRTPTPLVCTATQGSILNVDIPTQKLNRPINTNIYLPPCYDPKKEGGYPWLIM
                     LHGQAATNAQWLELGLTNAADDLITSKEIQPLVIVMPFEVTWTPGPRESQFDEALMED
                     VLPYIEENYAVCKERTCRAIGGLSRGGNWAVHLGFVSPEVFGIVGAHSTPLFYGELWN
                     IQAAAGLPPESIPLVLVDAGDKDAEKEKVQEFVTALKDAGVPYQFFEFEGRHEPSYWS
                     AHVADYLRWYAQAFTRLQSEK"
     gene            43434..43509
                     /locus_tag="P5516_02305"
     tRNA            43434..43509
                     /locus_tag="P5516_02305"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:43467..43469,aa:Pro,seq:tgg)
     gene            complement(43694..44788)
                     /locus_tag="P5516_02310"
     CDS             complement(43694..44788)
                     /locus_tag="P5516_02310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91303.1"
                     /translation="MTPRFIRNVLLKGLLLFALIHLAYALIPSDGLGRISAYNSLLRG
                     RERFPFGETPREAYNFSLYNFEAMFAAHRLDGTPKAADEFRVLVIGDSATWGTLLKPE
                     ETLAGQLDAAGLISADGRRVRVYNLGYPTLSLVKDLMVLERAMSYQPDLILWRVTLES
                     FPRDRQLESPIVAHNPQRVRQLIERYQLAITMPAGEPTFWQRTLIGRRRELADLLRLQ
                     LYGVMWTATGIDQLYPPSYPPAGRDLEADESFHHWQPPAFPEDGLALDLLQAGMKIAG
                     EVPVVLVNEPILVSNGENSDIRYNFYYPRWAYDLYRQTLAEASSANGWKYFDLWDLVP
                     QEHFTNSAIHLDAQGEEMLARRLIDAGLIQ"
     gene            complement(44785..46233)
                     /locus_tag="P5516_02315"
     CDS             complement(44785..46233)
                     /locus_tag="P5516_02315"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRT91304.1"
                     /translation="MTLLRILVLIGISLGVLVVCSIRRARRWRVVLLLVSSVVIIFWL
                     QPILPIRGLDFWLPCASLSLAGLGWALTSLPEERQRREILVTLGIVTATVLVIALTRY
                     LGSGGLLSASRPPQTLTVGIVVLVNLGLLLMAARISHRFKPALTLAIILVLLLFLVLK
                     LPALTLTTSALLRGFSGQSRELASALDVRWLGFSYIAFRLIHTLRDRQAGRLPQMALR
                     EYLIYILFFPAISAGPIDRSERFIKDLRGEFIPGALDFGAGSTRLMLGLFKKFVLADT
                     LSIVSLNASNAAQVDAAGWLWLMLLAYSLQIYLDFSGYTDIALGMARFMGIRLPENFR
                     APYLKSNLTRFWDNWHITLTQWMRAYFFFPVTRFLRNRARWMPAWLVILVTQLSTMVL
                     IGLWHGITMNFLLWGAWHGLGLFVQNRWSAGTQHLAARWQAKTGLSRAIHVLNTLLTF
                     IYVTLGWVWFTLPEPGLSTRVLVRLFGFGGGL"
     gene            complement(46384..47343)
                     /locus_tag="P5516_02320"
     CDS             complement(46384..47343)
                     /locus_tag="P5516_02320"
                     /inference="COORDINATES: protein motif:HMM:NF015722.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HRT91305.1"
                     /translation="MRAKVSETSAFRGLLLVIITLALCGCAEAPTATERVATFSPFVE
                     DEVRSSSQAAPTSNPTILSPATHQPDTPIPTPARTQMALDDGAIMLFIPKGDFLMGSL
                     KGEGEKDEYPQHTVQLNAFWIDQTEVTNRMYRACVADGVCNEPRRSSSFTREEYYGNP
                     QYLDYPVVYVNWFDAGRYCRWAGKRLPSEAEWEKAARGSEGFIYPWGNIEPGTLHANY
                     DLLVGDTTETGSYPAGASPYGALDMAGNVMEWVLDWYGEYTTSPYKTYDPSGPENGAH
                     RIARGGSYLFSPFLIRSAERYWVSPYYADDDLGFRCAFSAVDQ"
     gene            complement(47328..47717)
                     /locus_tag="P5516_02325"
     CDS             complement(47328..47717)
                     /locus_tag="P5516_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91306.1"
                     /translation="MMMKRWKTVLLLLGFCLFLVSCSGSNSPADVVVAYYNALVAGDA
                     SSLSQLSCADWEQQSKLMLDSFTGVKARVDNIVCSELENDGSSAAVNCKGKIIATYGT
                     EDQEIDLSNTTFLLQKQGGEWLVCGQK"
     gene            complement(47763..48647)
                     /locus_tag="P5516_02330"
     CDS             complement(47763..48647)
                     /locus_tag="P5516_02330"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HRT91307.1"
                     /translation="MSFIIWGVPGRQFILHTREFIMRSRLLLILLFVALLTTGAGSAP
                     EAVYSFSQAQNTERSPERWREWPVVPELSEHAVQILASAATNTSLNLRSISKIGDCQF
                     TPATFLAGYVKGDYPLPEEYERTAHYFKESMVRDSITAANGLGVSSVLNPMFAAGAGY
                     QECQRTETPLDCELRTRRPVVVLVALGTNWKPRQEITFEEHLRTVVETILASGALPIL
                     ATKADNIEEDWKLNLANARVAADYDIPLVNVWRAVQPLPNNGLQAPNNVYLTADGWVE
                     QNRAWLRMLDKVRVVLEP"
     gene            48700..49437
                     /locus_tag="P5516_02335"
     CDS             48700..49437
                     /locus_tag="P5516_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91308.1"
                     /translation="MAPKTPFLSIVFPAYNEELRLLETLQRTLAFIHTLPYPAEIVVA
                     ENGSVDGTFEIARSLSVLHPEITTLHLEKAGKGRAVQAGMLAARGRYRFICDVDLSMP
                     IEEVPRFLPPQIEGEDIVIASREAPGAMRYDEPWFRHFIGRIFNNMVRWMVLPKLQDT
                     QCGFKCFSDEAAEALFPLMTIPGWTFDVEVLAIAFKMGFRVKELPIPWYYHPHSKVRV
                     VRDSVRMASDLLKIRRNLRRGEYDLQG"
     gene            49421..50092
                     /locus_tag="P5516_02340"
     CDS             49421..50092
                     /locus_tag="P5516_02340"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRT91309.1"
                     /translation="MTFRVDPGLIPAAPDLAFEFALWENGLESVAGIDEAGRGAWAGP
                     VTAAVVVFPAYFALPQNLVFIRDSKQLSAEKRTALEPLIKQKAMAWSVGWASNQEIDC
                     LGILPATRLAMTRALELLKLTPQHLLIDALFIPEYPCGQTALLKGDQRALSIAAASIL
                     AKTARDKFMGEIASKISGYAFERNKGYGTRAHQAALSSHGPCSAHRFSYAPVKRSLKK
                     QQVEG"
     gene            complement(50122..>52651)
                     /locus_tag="P5516_02345"
     CDS             complement(50122..>52651)
                     /locus_tag="P5516_02345"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRT91310.1"
                     /translation="AEEALAALRRMAAPESQVLRDGKRISVPSSHLVPGDIVFMEAGY
                     YVPADVRLLEAVNMRIDEAPLTGESVPVQKNAALTLDPLSSLGDRKNTAFMGTVVSYG
                     RGKGVVTSTGMHTQLGIIARMLQSVQEEQTPLQRKLDQLGKTLGLAALVVCGLVFVVG
                     LVESIGAISADLIVGLFMIAVSLAVAAVPEGLPAVVTVSLALGMQEMIKRHALIRRLS
                     SVETLGSATVICSDKTGTLTQNEMTVTHIWVDGHMVKVTGQGYTPIGSFTIGEREIHL
                     DQYAGVRTALWVGALNNDAVLEETEREDGEKEFRMVGDPTEGSILVAAYKAGGEIAAL
                     NKAYPRTNEIPFDSDFKRMVTVHTVDDPRLNDISPYSEEKGRGSIVVAVKGAPDVVLN
                     LCTHYQPIDNGVQAVLDEDTKQQILAANDEMTGNALRVLGVAFRMVNEMPSQVEREEL
                     EKDLVFIGLIGMIDPARPEVNPALQTAARAGIRTIMITGDYPNTARAIAESIGLLGKG
                     RHVLTGAQLEAMSDDELKEEVRRTDVFARVSPEHKLRIVNALQANGEVVAMTGDGVND
                     APAIKQADIGVSMGITGTDVAKDTADMVLTDDNYVSIVSAVEQGRIIYSNIRKFVYFL
                     ISCNMAEILIIFLPTALGFLLFPQLTTEQLAPLAPIQLLWLNLVTDGAPALALSTEKG
                     DPDIMDQPPRAPKEPIINRQMLLGVTTQTIAISAATLTAFFTGLNYQGLSPENNLDIA
                     RTMAFVTLSSSELLRAFTARSERYPLLKQGIFSNKWMNIAVLFSLLLVLVVVYVPFLN
                     PIFKTVPLAWQQWQLLLPLLVVPSVVAELVKWFSYTHGKKTRLA"
BASE COUNT        12010 a        14766 c        13801 g        11874 t
ORIGIN      
        1 gttcaactgg cttgtatcct gatcctgctt aaagcatttt tgagatgagt tctagaaagt
       61 cctccctaaa accgccgccg ctgacagcgc cgccgcagaa gccttttcca gcaggttcat
      121 ctttcctctg accagcctga caatggattc tggcggagaa caggcgccaa aatcagaaag
      181 ctgatcactg gatatatttt tctgctgcat cgctctcaaa cactcgatca aataaaacgg
      241 gttgccgccc gtctggcaat gcatcttttt caccaggtct tcatcagccg tcttaccgaa
      301 aatgcgctgc acgaaaagac caatctcacg gttctgcagc ggagccaggc ggatcgtttc
      361 cagttttcgc gacctgctcc tgcgctgtaa caatgcgtct aattccgggt tgtccccttc
      421 aggagaaacc agaatgacca gcaacccgta ttgatcgaag aacttgtgtt caatcaagaa
      481 agaaatcaag gaaatactcg cctggtccat ccacatcgcg tcatccagaa caaacaatag
      541 cgggcggtta gaggcggtga tttttaaaag gttgaggaag gcataaaaga cattctccaa
      601 cacgcgttgc ggctctccag cggaaacagg cagcgtgaac ccctctgttc catggagtac
      661 atggtaataa aaatgagaaa gcaattgccg gtcagaaggc tcgagcgcct gccatacaac
      721 ttcctgaatc tgatcgcgta aaggctgtgt caatgccata aacggggtat ttcgccccag
      781 gggtagagag cggcagtaaa gcagagccgg gctgtacggc tgggtaatgt aaaattgttt
      841 caacagcctg gtcttcccga ttccctcttc gccctgaacc aacagaacgc cgcgttttcg
      901 cagcgcctgg ttgagcagct ccagttcatg ttcccggcaa atgaaaggag gctcaccctc
      961 ttcttgttca ggccaatccg ttggggggtg gttttcaatc tctttcctgg caatcgcgtc
     1021 cgttttaaaa gccgtgaacc gcgccggcag ctcttcctct tgctgctggt aaagtttttc
     1081 catgtaggtg atgtaatcga tcatttcctg aattcgcccg gtatcccgca ggcaaatgag
     1141 cgccaggtaa ttccaatctt cgtcaaacgg gttgatatca attccttttc ccaaccagat
     1201 taacgcttcc tccaggtttc ccccggaaat ataatgatct atcaggcggt caacgatctt
     1261 gatcctggaa tatcgataag cctgattggt caactccatc cagttctcaa atccagccgc
     1321 gctgttgagt gtcgtgccct gcataaaacg gtttgcgcgg cacagagcga aaccctcctt
     1381 caactgagcg accatccact cgggcagggc ggatcctctg tgcatttctg aactgctcat
     1441 cagcggcgtg atcaacctgt ccatctctcg ggaatccacc cagacgcgtt ctggatttaa
     1501 aaacaattgt tcgccttctg aaaaaatcag gtcggtaagc ccaatgctgg tgcgcagccg
     1561 gctcaaggct tctcgaaggt tttttctggc gatttcttct ggcgcatcag gccagaacag
     1621 attgcaaatc tcttcacgcg tgaccgggtt gttttgcgca gccagataga aaagtagcgc
     1681 ccggttcagg cggcgcttga tgaggaccgg cctgtcctcc agtgtcacct ctggcggtcc
     1741 cagcagcaag atttttagtt taggggtatc cataaatcga ttccgcgttt ttatgcaaca
     1801 gtttgccgtt cacgcgcccg agagagcagc tcgcgggctg cgcccaggct ggatagactg
     1861 atgctgccgt tcattaacgc gagttcttca atgcgttcnn nnnnnnnnnn nnnnnnnnnn
     1921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnncc aggctggata gactgatgct gccgttcatt aacgcgagtt
     2041 cttcaatgcg ttcatcttct cccagtctct tcaccccggt cagggtgcgt tggtcgataa
     2101 cgcttttgct caccttgtaa tgttcatcgc caaaggctgc cagctgcggc aggtgtgtta
     2161 cgcacatcac ctggtgtcgc ctggccaggc gccacatctt ttcaccaacc accgaaccta
     2221 ccctgccgcc aatgccctgg tcgatttcgt caaaaatgag cgtgggaata tggtcagcct
     2281 gtgccagcac atttttcaac gccagcatca agcgcgaagt ttcacctccc gaagcgatct
     2341 tgaccagcgg ttttaacccc tccccggggt taggagcgat gagaaactcc acttcatcca
     2401 gaccagactc tttaagatca accttaagcg cgtcctgcgg catcagtcca tccagttccg
     2461 gcagctcgtt gaaccaaacc gagaagcgcg cacccgccag gctgaggtca ttcagttcag
     2521 attccacttc ctggcccatc cgttgcgcgg cttgcctgcg caaacggctc aagtttacgc
     2581 ccgcgctcag tgttttaagg atcgcggcgt tttcctcttg ctccagttcc tctatccgtt
     2641 cgcctgcgtg ctgcactttt tccagttcac gcctggctga gaggccaaat tcattcaccg
     2701 cttcgaccgt gccgccgtat tttcgctgta attgatggat caactccagg cgctcttcca
     2761 cctgctccag tcgcctggga ttgaactcga ttccatcgag gtaggcgcgc aggtcacggc
     2821 tgagttcagt aagcgttgtg ctggcttctt ccagctggtc gacaagccca acctgctgcg
     2881 agtcaactcg cgccagcgct gcgagaagct tggaagcctg ccccaacagc tccgatgcgg
     2941 atgaggtttc aggcgagcct tcatccagga tgttgagaac ctcctgggtg ttggtggtaa
     3001 gcacctccgc gtttgccagg cggttgcgct cctgttccag ttcctgttcc tcgccgggct
     3061 gcaaacaggc ggcttcaatt tcctgcgcct gaaaggcgag cagttcctga cgacgcgcgg
     3121 cctcccgttc cagctcacgt aaagccttca actccttgcg aatttgctgt aaggcgtgaa
     3181 attccgtctg gtaggcttct aaagccgcgg cacaacccgc gaacctgtcc agcaggtcga
     3241 ggtgtgaacg aacgttcaaa agcgataggt gctctgattg cccgtgaata tccaccagca
     3301 ggctgccaag ctctttgagc aggctcacat tcacgctgcg accattcacc cttgagacac
     3361 tgcgcccttc agcgcggatc tcgcgcccca gggaaagaaa aggttcgcct tcaaataaac
     3421 cctcgcgctc gagtatggcg gaaacctgct cacggcaggc agcggggata tcgaacagcg
     3481 cttcaagcat cgcgcggtct tcaccctggc gcaccaggct gccatccacc tttccgccca
     3541 ccagcgccat aatggcatcc agaatgatgg atttaccggc gccggtttca cccgtgaaga
     3601 tcaacagccc gttatccagg gttagttcca gtttgtggat gattgcgaag ttctcgatgc
     3661 gcagctctgt cagcattccc agtcctctaa atgcggatgc ctttnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3781 nnnnnnnnnn nnnnnnnnnn nnnngttctc gatgcgcagc tctgtcagca ttcccagtcc
     3841 tctaaatgcg gatgccttta aggcggtaaa aaaccgtgct ggctgccagg atggcgtaaa
     3901 ccgccggcca aagcaaattc aacaacccac cagcggtatg gatatagaaa tttaacaggg
     3961 caaacagcgt gttagccact ggcacaaaca gcccgccaat cagcgtggcc gccactgcga
     4021 taacgggtat cggttttgaa cgccggcgtc gaattacaaa gcgcacggct tcggcgatga
     4081 tcattcccgc taccggcgcc agcaggatgg tgaaaaaacc gaacaaccca gcgataaggc
     4141 tgcccaggaa agaaagcaca cccgcagtga gaaaggtgag cgggtaatcc caccagcgcg
     4201 cggtttcaaa aatcctttgc tgccctttca cgcattcctt gcaacggtaa cctgtaggag
     4261 tgagcaccgc gcacgacagg caaattggtt tttcgcagcg gttgcagcgc agcagcgttt
     4321 cgcgttgggg atgtacataa cagtgcattg attctgaagt cgcttcttct gccattaaaa
     4381 atcaccaata gaaggatttt gttccatata gttgctcagg ttacggtaga agtaacccgg
     4441 ttcctgaaaa acgacgaatt tcaccgaaag gtcgctggca gtcaccgtca cgcggtcttg
     4501 attgctgata agcgccggtt cgtgcccgtc cacgctcatc acggcctcgt ggtcgctgtc
     4561 tactgtgatc accacgctgg cgccttctgg caagatcacc gcccggtcca tggagagatg
     4621 cggcgccacc ggcatgataa ggatattgcg cagttccggg ggcatgatcg gcccgcccac
     4681 cgccagcgcg taagccgtgg agccggtggg agtcgaggcg atcagcccat ccgccacgta
     4741 actcgccagc gggtagccat ctacctcggc gtgaatgcgg atggggcgca ccacctgtcc
     4801 gcgggcgacc accacctcgt taatcacctg gctggtgcgc aatcgttcgt caccgcgcca
     4861 ctgctgggct tgcagcatca tgcggttctc gaccttgtaa tctccagcca gcaaccgcgg
     4921 cagcgtgtca gcccagtctt ctcgcgaaag ctgcataaga aacccgaact tccccaggtt
     4981 gatccccaat atgggcagtc cctgcggagc cgcgaggtga ccggcgcgca gcatggtgcc
     5041 atctccgccc agggcaatga gcaggtcata ttccccctgg ctgatcttcc tgcgcaggtg
     5101 ctggttgtca gtggtggtga cagtactgct tacgccgtgc gacttcagcc actgctgaat
     5161 aaccgcgcct tctgacaggg tatcggtcga ccccggccgc accacgatcg caatacggtg
     5221 aacagatggc gtagacatgg tttctttatt ataacgaagt ttaatcgggc gcatgaatac
     5281 tcatcaccaa ccgtaacggg gtcatgacgc gtctggctgt ggtgttcata tgtcgcgcca
     5341 tcatcacgag atttaacagc cgacaatgta aagtaacggc tacgcagata acctgtgtgg
     5401 tcttttatgt ttcttttcag aagtgaagaa tttccccatg aacgcctggt gctactctgc
     5461 caatccggga tacttttcag agaatctctg cagcgcttcc tgcaaatgct gctcgcgcag
     5521 gctcagcgtc agatcggcgc gcgtgcgctc gataatgccg cgcaccaggt cggtctgacg
     5581 ccgcagccca ttggtgatgg cgtcggggta gtccagcgtc actaaaaagt tgtagatctc
     5641 gtccatggtg tttaacagcc gttcggcttc taccgagtat ccctggcgca gaatatccag
     5701 gcatctgcgg cgcagttcac cgatcgcttc cgccatccca ttgacataag tagcgtgttc
     5761 gatttccagt tcttccggtc gcggcaaccg gcgattctcg atcaacgcgc aggtaaggct
     5821 ggcttcagca aattccttga tcgcgtcctg ggtgtacccg gcgtgataga ggtcagggta
     5881 atccgccagt tcacgctgta aggtgagcac caattggcgg gcttcattga gcagcccatg
     5941 cgcttcgtct ttatcaccgc ggtgtatggc gcgaatggta agagcagaaa cccgtgtgag
     6001 ttggcgtgcc tgtaccagcg ccgcgtcacg cactttggtg cgttcatcaa agaccttgcg
     6061 cgtgctttcc acaatttcgt tgaattcgtt catgggctaa cccgattttg gcggttcggg
     6121 agggtgcacc gacctgaaaa ggtcttctgc cagccctgaa tcgccaggga gatagatttc
     6181 accgaaggcc gcctcgtatc gggcaatgtt ctctgccagt gcgcgtaaca gcagcttggc
     6241 gcctaccgga gagagcaaca cccgcaccag aacggtgagc cttttttgtc caggcaggat
     6301 tcgggagaaa tccaccgcca gttctgaggg ggtatggctg atgcgcgcca ggttgctgta
     6361 aaccggctct aattcatccg gcaactccat tgggggtaac ccaaggggcg gattttgcgg
     6421 tctgttgctc atgggagctc gctaaaacgg tatctcttca gagggagcga tatccagctc
     6481 ttcttcctgc ccgggttcgc cgccttcttg acgctgtgac aggaatttga ccgtctgggc
     6541 ggtgacttcg tatgacgcgc gcgcctggcc atcctgcccc gtccaaaccc gcgggccgcc
     6601 tgttttcgta tccactgtca atcttccttc gatcagtacc agtttgcctt tttgtagata
     6661 agtgttgcaa gtctccgcca gcttacccca ggcggtcacc ttgaaccaca ccgtttcttt
     6721 caccggtttg ccgctctggt cagagtattg acggtctgtc gccaccgaaa aacttgttac
     6781 tgcctgccct gtaggggtat agcgcatttc aggatccctg cccaatctgc caacaatcac
     6841 cagtttctga tacatttgtt cctccttaag gtaccgtgta aaaatcgtgt gcggttatta
     6901 ttcattttac aataaaaatc attaattcaa tcctgccaga agggagatat tattgacagc
     6961 tcagccgcgc gccgcccggt tcggcgcagg gtcgaaaaac cagccaggga ataatgttat
     7021 aatcttgcca gtgaatgcca taaaacctcg aagcatcctg attttcttgt tcatcctcct
     7081 ggcggcggct gttctggtaa tcacttcggc gttgatctca acaccggcac aggcgcaggt
     7141 gctttacttt acccccactg ctgatgacag cggcgcgatt tattacatcg tgaagcaagg
     7201 tgatacctgc gacagtatcg ctctggtcaa caacattccg gtggatacgc tgcgcaacca
     7261 gaactcgctg gactttgacc agtgtcgttt cctgcagatt ggtcagaagc tgctgctcgg
     7321 aaccgtgccc accgcggtca tcacccctgg cccctcgccc acccctactt ccatcctgcc
     7381 cacaccagaa ccggtcaaag gctttggcac gctgtgcatt tacctttata acgatattaa
     7441 cggtaacgcc atggctgaag aggaagaaac catgaacacc ggtattgccg gcgggcaggt
     7501 cagcatcact cacctcgggg gtgaatactc caatgtaggc acaactttaa gcacgggaga
     7561 agccctgtgt ttcaacgata tccctgaggg cgagtactct atcagtatcg ccatccctga
     7621 tggctataat cccacctcca gtcagaatgt gaccgtgctg ctgaaagccg gcgacacctc
     7681 caccgttaat ttcagcgcgc aggtcaacgg cctggcggaa agcccgcggg gaaacggaga
     7741 tgcgggcggg tctgttctgc ttgcggttat tggcgggttg atcctgctgg ccggagtagg
     7801 cgtgggcctt tacgcgcggt atatcctccg caaatagtca gcatcacctt gtaaaccgcc
     7861 tttgccccct gcccggttgt cagcggggtc tattcagcta tgcgctgctc tttccagata
     7921 tccgcctcgt tatggtaacc ggcttcttcc cagaaaccca acctatccac cgctgaaaac
     7981 tccagcccgc gcagccattt ggcgcctttc caaaagtagg gtgttttcag gtctgggcgc
     8041 tgcggaatag cgcccaccac accgcgcagc gggtaaccat ggtcaggggt gagaggggcg
     8101 ccatcataat gcgtggcaag gaggaagtta tctgccagcg ctatttctat aggaagattg
     8161 gcgctgaagc cgtattcagc gtgttgaata acaaagcgcg cctgcggctt taaccgaatc
     8221 agtctgtttt caatcagcgt gcgaatggat actccttccc agagcgtatc aaacttactc
     8281 cagcgcgtca cgcagtgaat atccatgatt aacgtggttc gcggcagttg tgaaaattcc
     8341 gcccaggtca ggcgcagcgt ttgctccacc tcgccccaca cgcagaagtc ccagtttacc
     8401 gggtcaaaac ccggcaccgc gccgtaatgt aataccggga acttctgggt gagaaattgc
     8461 ccgggcggca tacgtcccgc cgctttcatt tgctcttctt cagtcttgcg tcgaaaaata
     8521 ctgtccatat ttcacctgag gtcattatat ctgttttttg ccttacccag aagctgtttg
     8581 agacaaattt ggataaactc aaatttggac aaaaattgac atatatcctg cgcctggttt
     8641 acaattgagc ccacaggaca gcaaatgctg atatacgcgc aaagacagga cccatttgag
     8701 acccggttgt taaaacttca ccgggatgcc gccgaactgg taggcaacct ttcatatgat
     8761 gaggtgctgg aaaaaattgc cagcatcgcc ctggaacgtt ccggcgcagg ttacgccgcg
     8821 gttgccaccc tcgatgaaca tggcgagatc gagcgttttg ttcccgcggg gatgctgcgc
     8881 acccagattg agaaaatccc tcatccgccg caggggtttg gcttgatcag ggcgatgatg
     8941 aacacgcgca gcgccatgcg catccccgct atcagtaaag atcaacgcca cagcggcttc
     9001 ccgcctgagc atcccaagat gtcttcgttt ctcggcgtgc ccatcttcca ggggcagcgc
     9061 cagatcggtc agatctatat gaccgataaa gtgattacgg gtgagttctc agaagaagac
     9121 cagcgcctga tggaactgct ggccgcctac gccggggtcg ccatcgtcaa cgcgcgcctg
     9181 ttccgtgatt acgccatgcg cgatcgtgcg ttgacccggc agaacgaaaa cctttctctg
     9241 ctctaccagc ttgccagcac cctttccacc agcacagaag tgaacgaaat cctcgaacag
     9301 gggatgatgc agttgatgga ttacctgcgc ttggaagtgg gtgagatttt cattaagaac
     9361 gaggatagca ggcgcctcaa cctgctcatc catcatggtg aagcggtgga tagcctgtgg
     9421 aagcgcgcgc aattctctat tggcgagagt acagtagggc gggcagccaa gggcgatgtg
     9481 cccatcgttc tcaacctcac tgaacacgaa tacgccgacc tcaaccctct caccaaggcg
     9541 cgcgggttgc accagatggc ggtcattccc atgaaagggc ggcgcggcgt tgttggggtt
     9601 ttatgcgtgg cgacctgtca cccgcagccg ctgaacgagc ctgatgtgca attcgcccag
     9661 gccatcgccg cctggatggc aaccgccatc gaaaacatcc gcctgaacat caccgggcgc
     9721 cgcctcgcta tccttgaaga acgcgaccgc atcggtatgg acctgcatga cggcatcata
     9781 cagtcaatct acgccgtggg gctcacgctt gagcacgccc ggctgttaat gaaagacaac
     9841 ccggatacag ccaccagccg tatccaacag gcggtcaacg atcttaacag caccatccgt
     9901 gatatccgcg catacatcct cgacctgcgc ccgcgccagc ttaagaacga aaacctgatg
     9961 gagggcatca accggctggt gcaagaattc cgcgccaaca cactggtgga tgtcacgctc
    10021 aacggtccca aagacggggt cgccaccctg cctgaggccc aggcgctggc cctctttcac
    10081 atctgccagg aggccctggc caatatcgcc aaacacgcca aagccaaacg cgcatacgtg
    10141 agtgtgtgga ccacctccga acgggtgttg ttggaggttc acgatgacgg gcttggtttt
    10201 gagcagaata aaaccaaact ctccatcggg cacggacttt cgaacatgga aacacgcgcc
    10261 atcaacgcgg gaggtgaggt agacatctcc tcagaacctg gcggcggcac caccgtactt
    10321 gcctgggtac ccttccctga cccggatttc tccagcaacg accgcgtgat cgagtaaacc
    10381 gcccttacgg ttgccagtgg cgttccttta ggctacaatc ctcctatgag ctggaacctg
    10441 ctgggccata cctgggctga aaatctgctg cgaacacaca tcgcgaataa tgaagcgcgc
    10501 cacgcttacc tcttcaccgg cgctcccggt gtggggcgtc gcagcctggc cctggctttt
    10561 gccagcgccc tcaactgcac ccaaccacag caacccggcg attactgcga gaattgccgt
    10621 atatgcaggc acactcaacg tatgcagttg cccgacttaa gcgtcgtggc acctgaaagc
    10681 gaggggggca gcatcaaagt ggcacaggtg cgcgagctgc agcgcgccct ttcactgacc
    10741 ccttacgaag ccagataccg tgtggcgcta ttgttaaact tccagctggc gaatgccagc
    10801 gcgcagaacg cgcttttaaa gacactcgaa gaagcccctg agaaggttat cctgttgctc
    10861 accgccgatg ctgctgattc gctgctgcct accatcgtct cccgttgtga agttctccgg
    10921 ctgcgccccc tggctgttga agctcttgaa gacgcgctcc tcaaccgctg gcagttggcg
    10981 ccgcagcaag cccgcctgct tgcccaccta tccagcgggc gccccggcat ggcactgcag
    11041 atggcgcaag atcagcacct gctggagaaa cgcgccgcct gggtgcagga actgttacgc
    11101 ctgctgccag cgaaccgcta tgagcgattc agcgcctgtg aagcgttggc ccgcaaccgc
    11161 gaccagttgc gcctgatgct gcaggtttgg ctttcgttcg cccgcgacct gctgctcacc
    11221 gctagcggca gcagtgaaaa aatcgttaat caggatcagc tggcggaaat cgagcgtatt
    11281 gccggcggtc tgcccaccag gcgcccgttg gagatggtga acaccttgat cacatcactg
    11341 gaaaacctgg aagccaacgc caacttgcgt ttgctgctgg acaacaccct gttaaaaatc
    11401 cctcaaatca actgatatat tgaaggcttc cccgcaggct gcgctccaga cgctgaaaat
    11461 cagcggaata acgcagttct tcttttcgcg gtcgcggaaa ggtgacctcg atgtctttgg
    11521 agatctcggc ggggcgcggc gtgaagagca gcacacggtc agagagcagc agcgcttctg
    11581 aaattgaatg cgtcaccatc accaccggca cacgctgccc ttgccagatg cgcagtaatt
    11641 cgctgcccat gcgttcgcgc gtcagcgcgt ccagcgcgcc gaaaggttca tccaataaca
    11701 acaagtcagg ctcctggata aaggcgcgag ccagcgccac gcgctgtgcc atgccgcccg
    11761 aaagctcgga aggccagttg ctgccgaact cttccaaccc caccaggcgc atcatctccg
    11821 ctaccctgct ggcgctctcc tcttctgcca tccccagtag ttccaatggc agtgcgatat
    11881 tctgccgcac cgtgcgccag ggcataaggt tggcgtgctg aaaaaccaac cccacccgcg
    11941 gcgcgccatc cgcgccacca aagcgtatcc agccggatgt tggcttgagc agcccggcga
    12001 tgagccgcag cagcgtgctc ttgccgctcc ccgaaggacc cagcacgcac aggaactctt
    12061 cactaccaag atcaaaacta atctcgcgca gcgcctcaag cccgccttcc gcgccgcgga
    12121 aggtcatgcc cagctgcctg acttcgagga aaccgttgct cattcttatc ttctatggga
    12181 tgaactggtt gtcgtaaacc ttgctcaggt caagctcatc tttcagcagc cccattttca
    12241 gcaagatgcg ctgcatattt tcccaggctt gcggctgcga gtaacccagg cgatcgtctc
    12301 gccataaagc aatggagttg agaagcacct gcatttgcat ttcctggtcc gccgaggcta
    12361 aattctccac gtattttttg ctgatctcaa acgcctcttc gggatggtcg atcgtgtatt
    12421 gaatgccttt tatcattccc gttaccatcg aacgaaccag ttcagggtct ttttgcaatg
    12481 tcatctcatt ggtgataatg ccattcgcca ccaggtcaac cgcgtccgcg acccgcagta
    12541 atgtgatctc ttcacccagg cgctctaact gcaccggctc gttggccaca taaatcacca
    12601 ccgcctcgtc tcggtcagcg atcagcgatt cgacctgtgt gaagccaatg gaatccagtt
    12661 tcacatccgc ctctttcaac cccgcggagt ccagcagggc gatggcgccg atgtacgaag
    12721 cgccgtagag cccgggcagg ccaatcttgc gcccgcgcag gtcttccact gtgttgatct
    12781 tggcggattt tttcgccgcg atgcctaccg ggtattgctg gtaccaggcc atcacgtaca
    12841 ccactggcat ctgttgcgcg cgcgccagca gaacctgctc acctgagact accgcgaact
    12901 gcaggtcacc ggtggcggta agcacggtat tgtcattctc catgctgtaa tcaatggcaa
    12961 catccaggcc ggcatcccga aagtagccct tctcaattgc cacgtagata ggcgcgaact
    13021 gcacattggg gatgtatccc accggcaggc gcaccttccg cagtggcgcg gaggtgctgg
    13081 ttggttccac cgcgggcgcg atgacgcgtg tgaccgcgaa caacgcgatg agaatgactc
    13141 cgatgaagag gagaagaaaa aatgtttgtg atgtcttttt catgtgttcc ctcgcgaacg
    13201 agattttaac ccctatcagc cacccgccaa cgcgtggcgc gcctttcggc gagcatcgcc
    13261 aacccgtaca ggctcatcgc catcaaaatc agtgtgaaca gagaaacgaa caccagcgcg
    13321 gtatcaaact gccctcgtcc cacgttgatg agaaaaccca acccacggtc agcgccgacg
    13381 aattctccca ccaccgcgcc aatcaccgaa agtgccgccc caatgcgcag gccgccaaag
    13441 agcaccggca gcgccgctgg cagttccagg tagcgcaacg tttgcgcgtg tgtggcgcgc
    13501 attgaacgca ttagatcgta caggttggca ggtactgccc tgaagccgat gatcgtgttc
    13561 accagcaccg ggaagaacaa ggtgagcgcg caaataagca cctttgaaaa aatgccctgc
    13621 ccaaaccaga tgataagtag cggcgcaata gccacagtgg ggatggcctg gctggccacc
    13681 aggtagggag aaatgatctt ttccagcagg cgggatttcg ccagtagata acccaacgcc
    13741 atcgccacca ggctgcccgc cagcagaccc aacagcactt cggagagcgt tacccccgcg
    13801 tgaaagagca gcgtgccatc acccgccacc tgcagaaact tgttccatac cgcgcctggc
    13861 ggggggagta aaaaaaccgg cggcttgccc cagtcaataa tgagcgccca tactcccagt
    13921 gctaccagca gcgatattcc tgccagccaa ccgttatgac gcgccaggaa ttccagcgcc
    13981 ggttggctgc cgccgagcgt atcacccatt ggggttttca agtcatcatt cgtgcgcaaa
    14041 aaaagcctcg aaacgcgttg gttttcgggg ctggctgaca ggcgggtatt ctgcctgttc
    14101 tggctgctct tctttcatcc ggactgtacc gtcgaccccg gaatttcacc ggatcctgcg
    14161 cgtgtaatgc gctcgtgggt tatcaccacc gatcgggaat tgaggcgcga acctctcacc
    14221 ctgccccgaa gaagcgtatt gaattgagcg tattttatac cggtgcaggg gtgatgtcaa
    14281 gcagagctga ggggtgaacc ggtatgacct cgccctgttc cgataatatt tccttatctc
    14341 ttaaatgtat gcctaaatga aaatacggat tgaggtgaac cagtgaacga acctgcgctt
    14401 tcagttcaag atacccagca ctgatctgaa aggattcgct tgcttgaggg ttggttctat
    14461 ctatataacg aatttctccc tctaaggctg tacggatatg ttcgcccaat cccgttagga
    14521 gcggaaaact ccggcttgct ccatttgcaa ttaggctcgt cttcaggatg agttctattg
    14581 ataatcaaac tcccgtgtcc aattggacac gggagttcgt ttgttaaaga agccaggctt
    14641 agtttttcgg catgaagctg atgcccgcat aaccggtaat caggccgttg taggcggcta
    14701 cccacatggc gtctcccttg ctgttgaacc agtaatattc agttcgcctt gaaccatcat
    14761 cctgaatctg atcccagatg atcaatcccg gtgagccaag gatctgcgcc accagtgtgt
    14821 aaggcaactg ataattcatg tctttttgca gttgagccaa ctggtcataa gtgtcgctgt
    14881 cgaactccac gcccatatct acctgattga ccggcccaat agttttctta taaaccaggc
    14941 ctgaggtggt gatggagacg taagccttca tgtcgccacc gatatcaaag acataggtat
    15001 ctttgtgatc cgcgcgcggt ttgagccccc cggaagaact cttaagcggg ccgataatgg
    15061 gctcaagcgc tacgacttcg ttgatcggga cggagagata tttacggtat ggtccggtgg
    15121 ttaaaaagcc cttcccttct ggtacatcgc ctgcaaagtc cattccacct gcaaaaaatg
    15181 gggtcggatc cgtaggtcca taagatccta gccgcggcat gcccaccagt tctaccgcgt
    15241 caatttcatt ccatcccagt cccaattctg attggtcaat ggtgactcgc accttgttca
    15301 cataaatttc ggtggttgtt tcaatggaga cggtcaggta tgccgggcaa tactgcgttg
    15361 tttcagggga accttcgtaa acagagaatt cggtaccgtc ctcggcaatc acggtgatat
    15421 ccacgacctg cgaagggtta taactttgca ccacggttac ctcgaacgga atgacggggg
    15481 tttcatatgt cagttcaatc cactcgaccg tgttggaaga cgaagaagcc caggcaaaaa
    15541 tatcatcctg gcaatcttct acatcgggct cacccacagc ctgtttagca gaccagcctt
    15601 catcaccgta ctgtgaactg gctaccgcgc tcaccgccca ctgatgcagt tggccatcct
    15661 catcataggt ggagaactga ccgggttcaa agtcaaagtc atctggcgag acgatatacc
    15721 catcatcgta gatgtcttcg tcaaaactca tttccttagg ttcaaagaat ttaattgact
    15781 ttagcacctt ttcataattt ttgttgagcg ctttccattc tgcctttggc gcaaccccaa
    15841 gcaggacaaa atgttgcgtc ggttcaacca tcacaatcac caagcgcccc ataacatctt
    15901 catcttcgta atcttcaagc ttaatatcca ggccagcctt gccatccact ttcacctttt
    15961 ttggctttga aaattttgcg tctggtaaag attctttcat gttccccaga agcttctggt
    16021 tatccaattt ttcttcttgt tttgccccca tcatggcgat catcggtcct gtttcatcgt
    16081 tcccacctgg ggccatcatc tgggtgatgc cataaaactc gatcagttcg tagccttcaa
    16141 tgggcttgaa tgagtacccg ccgacttcag agcgatattc gcctgaagac gccgtgtcct
    16201 tgtctcgccc tggcaacagc ccggtcaggc tgcaggccat tagaaagacc gccagaacca
    16261 cgattaaaat gctctttttt ctgaagttat ttgtcatgct ctcctccttg tgattattct
    16321 atttcaattg gtgtttgctg gcagcaggta tctccgcctg actacccaag agcaactttg
    16381 atagaattat agcagaacct aactttttcc tgccttcatt ttcgcaaacc tcccgcatcg
    16441 cttattgaac gcaaaggaga tcaactctta aggattggat tgataatccg ttgacgcagg
    16501 gcgctggata gggtatcctt aaccccgcgc tccggatggg accggcgcgg cagagccctg
    16561 cgaaccccgt caggtccgag aggaagcagc ggtaaggagg ataccctggg cgccgccggc
    16621 aaacctgtcc ggacgcactt ttcacactct ggagaatgat gcagacaccc ccagtatgca
    16681 attatgaagg ctccgattac cagaccagtt tctgggaaaa aggtgggcgc gattatgaag
    16741 atggctgtga agccgtggcg ctcaagcgct tgctgccagc cgccggtagc cgcttgctgg
    16801 agctgggcgc gggtgccggt cgtaacacct cgcgctacag tggttacgcc caggtgaccc
    16861 tgctggattt cagccgtact caacttgaac aagctaaagc caggttggga acaggtgaac
    16921 gctaccgcta catcgccgcg gatatttacc gcctgccgtt cgtgaaggga ctgttcgatg
    16981 gcgccaccat gattcgcacg ctgcatcaca tggctgaacc tgccttggcg ctcgcgcaag
    17041 tgcgccgcgt gcttgagcct gaagccacgt tcatcctgga atacgccaat aaacgcaacc
    17101 tcaaatcgat actgcgttat ctgctggggc ggcaaaaatg gtcgccttat acgcgagagg
    17161 cggtggaata catcccgctc aattttgatt ttcaccccgc cgccatccgg gattggatgg
    17221 atggcgcggg attcatcctc gaacgcgctc ttactgtcag ccactttcgt gtgagttttc
    17281 tcaaaaaaac cctgcccgtc cggctgctgg tggcagtaga cgccttgctg cagcctaccg
    17341 gggggctgtt ccaattgact ccgagtgtgt ttttgcgcgc acgggcttta aaaacgggcg
    17401 taaaagcgcc acaaggcgca ttcttcgcct gcccggcctg tgccgctcca ttggacgata
    17461 cccccccatt gattcactgc cccgcctgtg agaagcaata ccccgtgatc gatgatattt
    17521 acgactttag gctcaacccg ccggcggagg gggattaact catgcctgat gcgctgctgg
    17581 atgacctgaa tccacagcaa cgccgcgcgg taaccgcggg tgacggcgct gtgctggtgc
    17641 tggctggacc aggctccggc aagacgcgcg tgctcacgcg ccggctggct tacctggttg
    17701 agcgccgcgg cgtgcccgct tgtgatgtca ttgccgtcac ctttaccaat aaggcagcgc
    17761 gcgagatgga aaaccgtgtg ctcaacctgg tacagcaaaa ccttgatggc atctggctgg
    17821 gcacctttca cgccatgtgc gctcgtctgc tgcgccgcga ggtcaattac ctgccttaca
    17881 ggtccaactt cgtcattttc gatgctgatg accaggagtc gctggtcaag cgcgcgatca
    17941 gggaattaaa tatcgacgat aaaacgcatc gcccggcagg cgttcacgct gccatctccg
    18001 ccgccaagaa cgacctgctg ctgccggaac aaatgcccct gcgcacctac cgcgatgagg
    18061 tggtggcgcg tgtgtacaag cgctaccagg aaatgctggt cgccaataac gcgctggatt
    18121 tcgatgacct gctgctgcag gcggctcgcc tgttggagca aaaccccggc gtggcagagc
    18181 gttacgcgcg gcgcttccag catgtgctgg tcgacgagtt ccaggatacc aacctggcac
    18241 agtacaccct gctgcagcaa atatgcgctt atcacaaaaa catctttgtg gtgggcgatg
    18301 aagaccagtc catctatcgc tggcgcggcg cagattaccg caatgtgttg cgttttcagc
    18361 aagattttaa agattgcgcg accatcctgt tggagcagaa ctaccgctcc acccaaaatg
    18421 tgcttgatgc cgcgcgccag gtgatcgacc gtaacggcaa ccgcaccccc aaacaccttt
    18481 tcaccgagcg cggcacgggc agcaagctgg tgcagttcac cgcggttgac gaggtggctg
    18541 aagccgcctt cgtggtggat actattcagc aatacttatc ctccatgcgg gcaaccgcca
    18601 gtgacttcgc tgtgatgtac cgcaccaacg cccagtcgcg cctggtggaa gaagccttga
    18661 ttcgcgcccg cctgccttat cggctggtgg gggcactgcg attctacggg cgccgcgaaa
    18721 tcaaagattt gatcgccttc ctgcgcctgg cagagaaccc cactgacgaa atcagcctgg
    18781 cgcgcgtgat cgccgtccct cccaggggaa tcggtcaaaa gaccatcgag atgctgcaaa
    18841 gcgccgcctt ccaggccaac atcagctctg gtgaggtact gctcgacctg gggagcaaag
    18901 gagactcttc tcccttctgg gctatcatcg ggcgctcagc ctctttattg gcggattttg
    18961 gcgcgcttct cgcggattgg cactctctta aagggcgggt ctcgctggtc actctctttg
    19021 accgcattat cagtgacaca ggttacgagc cttatatcaa cgaccagtcg gatgaaggca
    19081 atgaccgctg ggaaaacata caagagctgc gccgcctggc ctatgattat gacgaaaaag
    19141 ggttgactga atttttacaa aatttagccc tggtctcaga tcaggatacg ctgccgccag
    19201 aagctgacca accagccacg gcacgccaaa aagcggtgac gctgctcacc ctgcacgctg
    19261 ccaaagggtt ggagttcaac caggtgttca tcacaggttt ggatgaaggg ctgctaccac
    19321 acagccgcag ccgtgatgac cccgaggaaa tggcagagga gcggcgcctg ttctatgtgg
    19381 gtatgacacg cgcccgtgac cagctgtacc tgttgcgttc tgagcgccgt tccagttttg
    19441 gcaattggga gtacagcgaa ccatctcgct ttttggctga catcagcgat gagctgttaa
    19501 cctaccaggg caaacgcggg ggtttgccgc gtgcggcgca atttgaggag ttgcgttgga
    19561 caacaactgg cctgccttca acttattcca ggcggcaaag aaagccagaa cttcctgtgg
    19621 aaacgcgttt caaacccggc atgcgcgtgc gcaccgccgc ctggggtgag gggctggtgc
    19681 tcgaaagccg cgtcgattcc gatggggaag agacagtgga tgtgcatttt gaatccgtag
    19741 gttttaagcg cctgctggct tcgctggcga aactggaaat aattccatga cgaatattga
    19801 cacgtgttgg aacctggggg gatagaaatt acttaccttc gttccatgag attcgatcgc
    19861 actcattaca agcctggata attgaccaat gtcacccatt gttgctgcgc tccctatttt
    19921 actggttctc gtgttcatgc tcttccttcg ccgcggcagc cacgaggcgg gctttggcgg
    19981 ctggctggta ggcatcgcgg tagccttctt gttttttggg ctcaactgga acgtactgtg
    20041 ggtttcgcag ctgaaggctt tgctgctctc actgaacgtg ctcaccgtgt tgtggcccgc
    20101 cctgctgatg taccacatag tcgaaatgtc cggaggggtg cgcgccatcg cctatgccct
    20161 gcaagagatg atccctgaac ccggctggct gctgttggtg ctggcgtgga tgctttccgc
    20221 tctggtggaa aacctggctg gctttggggt gcctatcgcc gtcatcgccc ctatgcttat
    20281 tctgctgggg gtcatcccgg tgcgcgcggt ggctgccgct gcaatcggcc atagctggac
    20341 ggtgagcctt ggcggtatgg cgttggcctt ccgcaccatg acggatataa ccgccatgaa
    20401 tgaaagcgag gttttcccct ttgcggctgt gttgctgggg gtgacggtgc tgctcaccgg
    20461 gttgggttcg gcgtatgtgc taaaacaact gcattactgg aagcgcatct taatattgtc
    20521 gctggtggta gcgcttacgc aatattttat cggtatcacc ggggtcatcc cacttagttc
    20581 ttttagcgcc gccattgtgg gtatcggcgg aggattgttg ctcacgcggc gcccgccggg
    20641 gcagaaaatc cgcgccagta ccagcccaca attacggggt gggctgttct cctacggcac
    20701 cctctttggt agtatcttca tcatctccat catcaaaccg ctcaaccgat ggctggcggg
    20761 cgcacgggtg agttcctggt ttccgcaggt ggtcaccaac caagggttca ccactgctgc
    20821 cggtcccggg ttcgttttca aaccttttac ccatcctggg gtgtggattt tattcagcgc
    20881 gctggtagca gcgcttgtct tgccgcgcct gactcgttca ggcaccgccc atatgaagga
    20941 gtctttgacc gccacctggc gctcagcgct gccggccagc ctgggcaccc tctttatgat
    21001 cggtctatca tcgttgatgg agcacagcgg tatgacccag ctcatcgcac aggggctcag
    21061 ccaggcgctc agcagttact acccgctggt ggcaccgctg gtgggcacgc tgggcgcctt
    21121 cgccacaggc agcaatacca attccaacat cctcttcggg cagatgcaga agggtgtggc
    21181 agcgctgttg ggcatcagcc ctctcgttct gctggcggcg cagaccaccg gcggcgcttt
    21241 gggcagcatg atctcccccg caaaattggc cgtgggcggc agcaccaacg ccatgaaagg
    21301 gaaagaaggc gctgcgttgc gtcttacttt acccatcggc ctggcaagca cgctgttcat
    21361 cggcttgctt gcatggatcc tgttaaaggt attgtaagaa ggccccaggg tctgtccatc
    21421 tcgtaaattg gctgcctgta aaggggaacg accgggcgtt tcgtgcaagc ggtttacaaa
    21481 ccatcccgtt ctcgcgccca atccacccag gcgtgggagt ttcaactgaa cagagtgaga
    21541 atctcttgca ggttctccgc gcggtattct tcgatgcgcc cggtgtcacc cagcagcgcc
    21601 acgcgcgggt cgagcggcag cttgagccag cgctgcgctc ccaccgccgc cgccagttct
    21661 tccacgtggc tgggtccgaa aatctcgtga ttgcccccgc catccgggca ggggtagtag
    21721 ctcatgttct ccaccacccc cagcacggga atgttgagct gtttgagcat ggaatccgcc
    21781 ttgcgcacca ccatgcccgc cagctgctga ggtgtagaaa ccagcaccac cccgttcact
    21841 ggcaaggtct gcatcaccgt cagcgccgcg tcagaggtac cgggcggcag gtccaccagc
    21901 agcacgtcca gcctgcccca cagcacgtcg cgccagaact gctgaatcga acccgcgatg
    21961 agcggcccgc gccagataac ggcggcttct tcggtgggca ccaacaggtt ggaagacatg
    22021 accctgatgc ccagcccgct gattgccggc aggataccct gctcgctgcc gcgcaacccg
    22081 cctggcggta acccaaagag cctgggaatg ctgggcccgg taatatcagc atccaaaatg
    22141 ccgatctttt tgccctgtcg cgccagctcg caagccagta aggctgtaat ggaggattta
    22201 ccaacccctc ctttaccgct catcacggcg atgacgcgat cgattttatt gaacttgttc
    22261 aattgcggta actccgtcgc tcccggaggg aagagcgctt tgcgctcctc ttcgctcatc
    22321 accgactggg tgatgttcac ctgcttcacg cccggcagct tcatcacggc ttcgcgtgcg
    22381 tcgcgctcga tcttttgttt taacgggcag ccggcaatcg tgaaggccag ggtaaaggcc
    22441 accaccccgc cccgcgaaat ggtcacattc ctgatcatgc ccagttccac caggctgcgc
    22501 cccagttctg gatccatcac ccctgataaa gcgtcatata cttgtttctc agtgatcata
    22561 ttgctccaat acacaccatt cattatctgc tggggatgat tataatggat acaaagaagg
    22621 aggttagtca atgaaacgct cgcttttctt atcgctgctc ttcgtggttg ctgctgtatt
    22681 aatggcctgc agcgcgtcgg ttgacctgtt cggaaacaag gtcaaaccaa ccctgaacat
    22741 ccctcctacc caaccgcccg ccattgccac ctcctccaca cctctgcagc ccacagtgat
    22801 catcgcactg cctcctccac cgtcgctcga aacatcaatc actgtttact ttatggatga
    22861 ggaccgtttt atcaatggca ccgaacccta cgaaattgcg gtctcacgca ccaccgccag
    22921 cgtcaacctt ccattggcag tgctgcaggc ttacttcgcc ggtccgactg tggatgaata
    22981 caaccagggt ctgcgtatgc ttggcagcgg cttttcttat gtgcgtgacc tgactgtcga
    23041 gaacggcatc gcgcgcgtgt acttgggcgg cgagtgcgcc aacaacggcg cggcttactc
    23101 tgtggcggcg ttgatcttca agaacctgca gcaattcccc gagatcaccg cgatcaagat
    23161 ttatgacgag aacaactcaa ctcttgaccc cgattccgct cacagttcgc tgccttattg
    23221 cctggagccg taaacccgtt tctatcataa aaatgtgtca gccgcggctg gcagccggtt
    23281 tttgcgactc aaccgccagt acgtaattgg tataatcctg ctgatgacca tcaccatcct
    23341 atccgttctg ttcgccgtca ccctggtttt tttagcgctt agccgcatgg ctgtggaacg
    23401 cattgaaact caccattatc tgcagcgggc ggatttcttt caacgttacc cggtgcagca
    23461 gggcgacatt gtctttttgg gggacagtat caccgacggt ggctgctggg aggagttgtt
    23521 cccgggcgtg ccgctcaaga atcgcggcat caacgcggat aacacgcgcg gcgttctgga
    23581 gcgcctggat gacatcttgc gcccccaacc ccgcgccatc tttttgctca tcggcaccaa
    23641 tgacctgccc tggtacacct accttaatga ccgggcgatt cttcacactt acaaccagat
    23701 cctcgaccgt tgccgcgccc tggctcctga tgcggttgtg cacgtgcaat ctttactgcc
    23761 aaggcaaaga atttacgccg ccaggatact gcgcatcaat gcccatcttg aaaccatggc
    23821 ttcacgacac ggctacccct tcattaacct tttcccggct tttgctgacc ggcaaggcgc
    23881 gcttcgcgct gacctgacca acgaccacct gcacttgatg gcagaaggtt atgcgctctg
    23941 ggtaagtatc ctcaaaccct atatcgaaca attgcataac gaaaatgtaa actcagcatt
    24001 acccagccct gggtttaacc cgtaaccgag ccaatcaaac tgggtttaac tggttacgtt
    24061 cctggtcata ttcattcgca tcatcgccgc cgcaaaaata tgccctggtg caccggcagg
    24121 aatatgcctt ttgggcggta cgcctggcat aacaggatgg tattctttga cggtatcctg
    24181 atctattaaa ggtaggttta ttctatgtct catttcaccc aaaaatcgct cttctggatc
    24241 tcgaagccag tggtcaagac ttacactaac gccatgctca agatggatgt gcaccaaaaa
    24301 acgccctttg tctccggtgc caagatcatc gcgcctaacc atcccagcac cacagatcct
    24361 ttctttgtcg ccgccatgtt ggggcagcaa tcttacatta tgattaaaga cgtgctcttc
    24421 aacgtgcctg tgttaggaga gtatctcaag cgctcaggtc acatcaaagt ggctgccggg
    24481 caagggcagg aagccatcga ccaggcgctg gcgcacctgg cggtgggtca taccatcatg
    24541 atctttcctg aaggcaaggt cagcccggtg aagggtggct tcaatcaggt tcgttcaggc
    24601 gtcgcccggc tggcgattgc cagcggcgcg ccggtttacc cggttgggat tcacctgctc
    24661 aaggaacgca tccacgcgat ttcatccaga gtgaaaggtc gggatgaaat gggttactgg
    24721 tacctgcgcg ggccatacaa tatcagcgtg ggcaaaccca tgcgttttaa tggggatgtg
    24781 gacgatcacg aatttgtgcg cgaaaccgcc cgactggtga tgcttaaaat tatgcgcctg
    24841 gcttacgaaa gcgagcaacg ctggtaccgt aaaactagag cagtggtggg ttcgctggaa
    24901 accgcctgaa cccgtaacaa ccataaagaa aaaccgcgcc tttttttgcg cggtttttca
    24961 tttgtcagag caaggtatat gagtacaata acattagatc aatcaacagc aagcaggaga
    25021 gaaaatgaaa ccccaacaaa tcctcgccgg cccgggcatg cttttagatg gacagggaaa
    25081 cctcatacag gcaggttggg ctacccaacc agttttggat gccaacctgg agaacgttca
    25141 tttttacgca ttccgcccgc tgcaaaaatt acggctgaag ctttgggatt attacgccat
    25201 caccacgccc acccatttct tctcttttac cgtcagcgat attggttacc tgggcatggt
    25261 ctttgcctac gtgatcgatt ttaaaaccgg cgccttcaaa gaagaaaccc tggcgttgcc
    25321 cttcggcggc ggggtcaagt tacctcgcaa cagcacggaa ggggtgactg aatacagtaa
    25381 gaaggacctg taccttcgtt ttcgggctga aggagaagtg cggcagcttt caattcgttg
    25441 gccaggcttt ggcggcagcg cgctcaacgc tgaagtggcg ctcacctgtc cggctgacca
    25501 tgaatcaatg aacattgtta tccccatccc tggtaagcgc ttctactata accgcaaggt
    25561 caactgcatg ccggctgaag gctgggtgga gtaccagggg acacgccatc tcattgaacc
    25621 aaccacctgc ctgggcagcc tggattgggg gcgcggtgtg tgggagtaca gctctttttg
    25681 ggtgtgggcc agcgcttcag gttttttgcc tgacaggcgg cgcatcgggc tcaatatggg
    25741 ttgcggattc ggagatacca gcgccgccac ggaaaattgc ttcatcctgg agggcaaggt
    25801 gcataaacta agcgagctca aattcaccta tgataaccgc aacttcaaag cgccatggac
    25861 catgagctct ccggacggca ggcttgagct ggtatttacg cccttctttg aacgtgttgc
    25921 aaaaatcgac gccctggtat taaagagcga agtgcatcaa ttgatcgggc gttataacgg
    25981 aaaggtgatc actgacacgg gtgagaaact ggaaatcgtc gacctgatcg gctgggctga
    26041 agagcataac gccaaatggt gatttgttaa aatgatggtc tttgaaaaat agaaaagtga
    26101 gcgttgtctg ctctctttgt gaagccgttg gtgcacgcca acggcgttcg accctgatct
    26161 accagcacag tccggcagag ggcgcttaca gcgctggcag gcggttgggc ttcttctccc
    26221 gagcgcgcaa agtgaaatat caagtgggtt ttttattgta aggtgagaac agaaaaggtg
    26281 atttcaggtc tctctgaaca tagtgcccaa ttcatttata attagttcgt tcatgtttgt
    26341 tcaaggagaa aattgaaaaa actcaccatc atcatgccgg tttataacga agaagagtca
    26401 ctgccgttga tcatgaacaa ctggctggaa ctgtgtgccg catctcattg caggctggtg
    26461 gtggttaacg atggctcaac cgatgggagc cggcggatcc tggataacct tcaatctcag
    26521 catgatttta ctgtcattca tcacaaggtc aataaagggt acggcggtgc cattaaaacc
    26581 ggcttcaggg cggtgaagac agagtacctc gtcacgattg atggagatgg acagcatctt
    26641 cccgcaagcg tgatccatat gtatcagaag atgattgaag aagattgcga ccttctgatt
    26701 ggcgcccgtg aaaacgaaac gaagttcacc gctcgtggac tgggaaaatg gatcattcgc
    26761 agtatatcta aactgctttt gccgaacaaa attcgagatt tgaactcagg gttgaagatg
    26821 taccgtgctg aattggcgca gcgttacatc aaagtctgcc ccgattcaat ggctttcagt
    26881 gacattatcg ccctcacttt tatcgcggaa aaatgtaaag tgatggaaac ccccattcag
    26941 ataagccccc gcgcgggcgg taaaagtaag ataaaccttg ggtcagcagt ggacacggta
    27001 atagagatta tcaacattgt tgttttcttc aaccccttgc gtgtttttct ccccctctct
    27061 gctttcttcc tcgttgcggg gttagggtgg ggtataccga ttgtgttaaa agggagaggt
    27121 gtaagcactg gctctttgct ggcctttacc atcagcggga taattctcct tttaggctta
    27181 atcgccgagc aattgtcaca gattcgcaga atactgatag aggaatgaac cttctttaat
    27241 cagcccgttc cgccagcgat cacctcccgc tggcgctgcg gtgtgttttt cctggaaatt
    27301 actttaatcg attaaaaagg gcaaaacgga tgttcccgct atcaatcatt tcatagtcgc
    27361 tctcgatgta acgctgaatg atcacggacg cttcacttga agaatactca tccgcgctca
    27421 ttaggatgaa agtgggttgc cattcaacaa tcgtttgcgg tttgaatgta tcaaaattca
    27481 caacgcgcac atctgaccag gttaaccgtt catcataata catgttgaac aggtcaatga
    27541 actcaacatc acgactgctg tagttaaggt cgctgagcgc atcctcaaag accaagagct
    27601 ttattttttc ctctttaata ttggcgctat cgaatacttc aattgcctgt aatcgctcat
    27661 tcatttttcg cacattggtc tgctcgaaga aataattgag agtgttgtca tataacgggt
    27721 atgcgataaa aataacaaag accaacaaag gggcaaagac cgttttattc gaatagtgtc
    27781 gaacgagaaa aaccaggatg attaaggcaa ttaacgtaat cgggtaaacg gtcgacgcga
    27841 gaaaatcagg ataattatag ggaccagcct gactcaccac tacaaggatt ttccttcctg
    27901 ctataaataa cagggcagcg gcgagcattg agaatgccag ccaggagaat tcttttttgt
    27961 tcctaggaaa gtcaaaaaca atcagttgcg gggcaaaggc gcacaccagg ggcagaatgg
    28021 gaaaaacatg ccgccctatc actccccagg agctgaagcc aattatcacg cttaaaatta
    28081 caatcaactt gagcggaata agccagggaa gccgcattga cggaggttgt ttctttagca
    28141 atttgatccc agaaaaaagg taggctaaaa aaatgtaagg ggtgctttcc aaaaaaaagc
    28201 cagtgaacca gttggcaaca gctttcgact cctcgccaat attgcggtcg atgtaggttt
    28261 ttttaaattg aaagtactcg ctaagtcgaa gaccaaacca ggcgtccttt aacaaaaggc
    28321 cgtttaagag catgaacaac acaacccctg caagcagacc aatcataaag taacccaacc
    28381 gttttaagca gagcctgacg tttataccgc cttctccatc gaacaagaac ccaatcaaca
    28441 aaacggctga agcaagggtg gtctcttttg agcgaaaagc cagaaagaaa gccagcccca
    28501 ttaaagaaag aaggtaaaca ggtttaccag gctgcctgaa gcacagaatg taaagcacca
    28561 cggtcaaccc aaggaatata cccgcggtaa ggtcaaccga ggtatttccg gaatacaccg
    28621 agaggagcgg tatggtgaga tacaacaaag tcgaaatacc ggcgtgcaag atgttgctgg
    28681 ttttcgtggt cagccttgaa atcacatagg tcaaggctgc cgaacccgcc actaaaaaac
    28741 cccagtaggc ctttacccct tccaagggtc gatcagcgat ctctacaaaa agtttctgaa
    28801 aaaagatgtg cgtatagcgg ttaataatga atgggtcttt gacgttcagc aagccatgat
    28861 taatgtacat gacttcatca gaatagatcg gtccaccagc gtatttggcg aggaagatac
    28921 ccagtaaaat aaaaaccgcg gcgctgatga tgatttctgc cagtaaaaaa acctcattta
    28981 atcgacattt ttttattccg ctcgtgaatg gcatgtgttg ttcaaaaatc ctttcagggt
    29041 ttatctcaat ttaaaaaccc gccgaagcag ctgggagaac gaccttgcgg ttagattata
    29101 ccttctgttt cagcggacaa aaaacccatc caaccccctt tgtgagattg gatggatcag
    29161 cgagtaatac ggttcgataa caagaattgg ctttcttaat taccctttcg ctttttacac
    29221 ccaacctctt aactgcatgg ccttgacgac gcggtcgata gccaccatgt aagcggcgtc
    29281 gcgggtatat accttgcttt tctggctcat gtccagcaca ccagcaaagg cgattgtcat
    29341 cttggtatcc aacttctcaa gtacttcttc tttggtccag tagtagttca tatcattctg
    29401 taccccttca aagtaagaaa cggtcacacc accagcgttg cacagaaagt ccgggattaa
    29461 aaatggaaaa ggacacaaat tggatgacat aatattcact tgtgatgatc agaacctcaa
    29521 atccctctat acaatgactt tgcccgacgg tagactcgcc ctggtgttta cgcctttcta
    29581 tgagcgcgtg gaaaaatctg acacactggt attgaagagc gggagtcagc agatgttcgg
    29641 gcgttgcaag gtgaaaagat taacaatatc aggtgaaaag ctggatatta tcgacttgat
    29701 cggttgggtt gaggagtaca acgccaaatg gtaatctgtt cgaattacgg tttttgataa
    29761 atcgaaaaat gatcgttttc cgccaggagc gtaaacccgc tggtgattga agggattcgc
    29821 ctcttatcca ccagtaccag ggcaccatcc ggcagctgct gcagcgaaac gacgggtacc
    29881 aggtcgcggg tcttgttcac gccgaacaac gcgtactcga tgatgtcctt gtctataaaa
    29941 aggtataatt tttcatgcgg tttcacagta ctgttgacaa actcgacatc cgcaatggca
    30001 ctggtgcttt gatggaacag gttttcgtaa taagactgct gcctgatatc cttacggtcg
    30061 gggctggtta gcaccaggtc ttcgatcacc cggttggcga tgtgcttggc ggcaatccca
    30121 ccgataaaac ctgaatcgcc ccacttctcc acctttttcg cctggaaaga gtatagcgat
    30181 tgctgcgtaa ccaaaggccg ggcatcattg ataagcaagg ttgatattgc gaggtagaag
    30241 gaggcgatcg agagcgccag cgtcacaagt ccgctccagg ctcgcttttg gggaatgagt
    30301 acagcgaata gcggagtaaa taccaacatc ggcaggataa ggtaacggcc gtttgtaggg
    30361 ctccagccgg tgatgagcac cgctaccatc aggaaaacgg tgagcataaa aacgagggat
    30421 aaccactgat agcgtttgca caacttgcat ttaccggtta aaaccaagac caccgcgata
    30481 ggaaaaagca caaaagacag ggggccaaac caggcggagt cctcgttcaa tgcgggagct
    30541 tccgcaaaag aaaagtactc atcgtcgcca tcagagatat aatcgcgcac ctcaagatcg
    30601 aggttgagcc attcactctt tgcgcgaaac gcgtgcgctc tcgcttcctg ggcggcgagt
    30661 tttaagcgcc ccgtcaaccc gtccaggctg accgcctgat agagatacct gccgctattt
    30721 gtggcgtagt gcaacccgtc acctggcttc gagaacggta gccggtaacg gtagccagca
    30781 aacatggaag attccaagtg gtcacgctca accgcgttct ggatgaactt gtatgaggcc
    30841 agcaacataa aaaaggccag gaacacaccc gcggcgccga ggataacctc cacgcgagtg
    30901 tgatttttta tgaacaatat cagcagggca agcaggaaga aaggtaaaaa gagaaacgcc
    30961 gtctgctttg aaccaagcgc tgccgccaag ggtacgaacg atagatacag gaagaaagac
    31021 tcttgctggc gaacgtacag aagcaggaac caaaccgccg ccaggaccag cgtcgccacg
    31081 agcacatccc cctggtacga gaaggtctgc agcagcacca ctgggaaaga aaggccgagc
    31141 agcccggcaa aaagcgctgc tctcctggag gcgcccagga gcatggcaat ttcgtagatc
    31201 agcaacacca ctgccagtag cgccagccag ggcaccaggt aaaacaactt ctccgacctg
    31261 ccctgtaaaa agagcgatgc cccctgtata gagatgttga ccgggtagtt caactgagtc
    31321 accgtcaccg cgttccatga agccaaagaa ccgtgctgca gccagtaata aatgcgcggc
    31381 aggtgagtgt gcaaagaatc agagttgttg ataggcgcga gggcgccgat ataaagacac
    31441 agcagcagga taccggagac cagggcaacc aggaatattt cccagccgta taagcgccgg
    31501 aattcaaact tgagcttcgg aatagggttt ttgaatacct tttttggggg atcccatatc
    31561 aacatcgcca gagccagaca gaagagcagt tgcaacagca ggtacatcca gcggctattg
    31621 agcattccta gcaggctcaa aacctcagcg atcagtacgt tcagtgtaaa gcttgaaaga
    31681 aagaaaatta tgactgagtg tgaaaatgaa acgctcttga agattcgcag gctcaacagc
    31741 agggcgagga atagatacac aagagcggaa aagatcaaca tggaaaacct ctgagaaatt
    31801 taccggtctt catgccgggt gaaggactgg tgagagggga accctaaccc ctccgcgttc
    31861 gatccgttca tgcagccaca tcattagcgg tcccttcatg ctcggcttct taggcatccg
    31921 ccgcaccagc ggttcaaccg acacgctgga ggtatccaac gtgatggttt gtatcaggaa
    31981 ctgcattgcc tggataatgg acggtacggc aataaaaacg atgccagtgg tggtgaaaac
    32041 ctcactaagg tcgggttcaa gccacttcac tgtgccccaa attcccccca aaaaaaccag
    32101 catcagactg gcgatcagaa aaaaagatac ataagcagga atgaacacag cgatcagcgg
    32161 cggggacgcg ttcgcaacgt tttctttgtg aatttcaccg agcatgcact tattatactt
    32221 gtgttgccta ttgaatccgg caaaaattaa aacaacccct gttgaatggg gttgttggtg
    32281 aaaattgaga gagacttaca cccaacctct taactgcatg gccttgacga cgcggtcgat
    32341 agccaccatg taagcggcgt cgcgggtata taccttgctt ttctggctca tgtccagcac
    32401 accagcaaag gcgattgtca tcttggtatc caacttctca agtacttctt ctttggtcca
    32461 gtagtagttc atatcattct gtaccccttc aaagtaagaa acggtcacac caccagcgtt
    32521 gcacagaaag tccgggataa caaagatgtc gctctgtttc agcacctcat ctgcctcggg
    32581 cgtggtgggt ccattggcgc cttctgccaa tattttgacc cgcggtgata tctgcttgac
    32641 cgtttccccg ttgatttgcc cttccagcgc cgcgggaatg agcacatcca cgtctttgct
    32701 gatccaggca ccgccgtctt cgatgacgta tccggcttcg cgagccttga ccttgtccac
    32761 cgtgccgtac tggtcagtga tggtcatcag gaaacgcggg tctatgccgt ctgccttgct
    32821 gacagtgtaa gaaaccttgt cctcgcggtc gtagtaagaa atacagatga ctttgccttt
    32881 gagcatttca gtaaacccaa tagaggcgta ctgcgccaca ttgccaaatc cctggataga
    32941 cgcggtgcat tttgcaccat ccagtttgag gtgtttcatg gcttcgcgca cattgataat
    33001 gacgccaaaa ccggtagcct cggtgcgccc gctcgatcca ccaccgccca gcggcttgcc
    33061 ggtaataacg cccggtgtga actctcccac cagtttggaa tactcgtcca tcatccaccc
    33121 catcatttgc gcggtggtgc ccacatccgg cgctggtaca tcctggcggt tgccaatatt
    33181 acgccacatc acatccaccc atccccttac caggcgttct ttttcacctg tcgaaagggt
    33241 tgaagggtca actaccacgc cgcctttgcc gccgcccagg ggaatatccg cgaccgcgca
    33301 tttccaggtc atccacgtgg ccagcgcgcg caccgtatcg agcgtttcgg cgggatggaa
    33361 gcggatgccg cctttatttg gaccgcgggc gtcgttgtgc tgcacacgga acccctggaa
    33421 catgcgcaaa ctgccgtcgt ccatgcgcac cggtatgcgg aaagaaaatt cacgcaaggg
    33481 ccagcgtaaa atttcccgca cctgcggatc caatttaagc aactcagcca cattatcaaa
    33541 ttgcttttga gccatttcaa atgcattaat cgaggttgcc attcagttca ctcctttacc
    33601 agttggaatt gaaattgatc agaagtcaaa aataaagcgg gcacaccggt cctgtgccca
    33661 cattcaagcg tgatgattga atcgatctta cgcacaaatc cctccgcatt tcagtgcaat
    33721 aatagaatat acgagaatga aaagcgcgtc aagatgacga acatcaggtc tttcgcaggg
    33781 ggatgatgca gggtattgcc ggtcactttt ctacccgcgc ccggcttcgg tcagttgttt
    33841 ttcgagttct tctttcagca ctgcagggct ggctttccct ccggcggcgc gcatcacctg
    33901 cccgaagaac cagtttgcca gcgtctcttt tcctgccagg taactcgcca gctccttctg
    33961 gttggttgtg agcacttcct tcaccaggtc agagataaaa ccagtatcag tgacctgcgc
    34021 caggcctttc tcgctgatca cctgctgcgc ggaggaaccg ctggccagca tctccgccag
    34081 tacggtcttg gcggtgttct ggttgatgcc gcctgcttcg attatgtcca acagttccgc
    34141 caggctttgc gggctaacgc ggatatgcgt aatttctgca ccagtcgagt tgaggtgcgc
    34201 gaaaagctcg cctgtgatcc aatttgccgc gctgcgtgct gatgcctgct taagcgccgc
    34261 ggcacagtct tcaaaatatg cggcaatatc actttccgat gtaaggatgg cagcgtcagc
    34321 cggtatgagc ccgtattgtt gctgcagccg gcgggtttta tcccgcggca gttcgggcat
    34381 acgctgccga atttgcgcta tccaatccgc ctccaccgca agcggtggaa tatccggttc
    34441 agggaaatag cggtaatcgt gtgcctcttc ctttgaacgc tgcacatacg tgcgctggtt
    34501 tggttcatcc cacccaagtg tttcctggct gaccgtttca ccgttttcca gcagagacgc
    34561 ctggcgcgcc agctcatagg cgatggcgcg ttccatcgct ctgaaggagt tgagattctt
    34621 tatttccacc cgggtgccaa gctggtcgct tccggcgggg cgaatagaca cattcgcttc
    34681 gaaacgcatc accccttttt ccatattgcc cgagttgacc cccaacgcgc gtagaatcgc
    34741 ccgcagacca gttgcgtagg cacgcacttc ttccaccgag tgcatatccg gctctgaaac
    34801 gatttcaagc aggggtacac cggcgcggtt caggtctacc agcgagcagt ctccgccgtc
    34861 atcggtgata tgggtgagtt ttccggtatc ttcttcaaga tgcgcccgcc gcacgcggat
    34921 gacacgttcg ccttcggatg tgtcaataac cagccgacca ttctccgcca gcggttgttc
    34981 gtattgcgaa atttggtacc ctttgggcag gtcagggtag aagtagtttt ttcgagcaaa
    35041 aatggaggtg tggttcacct cgcattcgag cgccagcgcc acgcgcacgc catattctac
    35101 cgcgcggcga ttgagcaccg gcagcgtgcc gggcattcca gcgcacacag ggcatacact
    35161 ggaattgggg cggctggtcg tggtatccac aacgcggcat ccgcagaaca ttttggtctg
    35221 cgttgccagt tctgcgtgca cctccagccc gataaccgct tcgtattgca tgatgtaatg
    35281 gattttatca cagccggtgc catccattac cccagcacag atgcattagc gtgttaaaat
    35341 atcagtaacc atcacgcact gccagacgag gtgacgtatg ctgttgtgta tcgacatcgg
    35401 caacacgaat atcaaacttg gtttgtatga aggagaaaaa ctggtcactc attggcggat
    35461 ctataccgat gagcataaac tggcggatga atatgcagta ttgttactct ctttatttaa
    35521 aagcgaggat atcaaaaatg aagccatcag cggttgcgcc atttcatcgg ttgtgcctga
    35581 gttgaccatc gccttcagag acctggtcag aaggcacctc aacctcgaac cagtgattgt
    35641 gggaaacgtt aaacgacccg tcattcacat taacacctcc aaccccaaag aagtcggtcc
    35701 tgacctgatt gccaacgccg tgggagcagt ggcgctttat ggtgcgccct gtattgtcat
    35761 cggctttggc accgctacca ccttctgcgc catttcagcc agtggagacc ttgaaggtgt
    35821 cgtgatcgct ccaggtgtta ttacggctgc ttcttcgttg tttcaggctg gtgccatgct
    35881 cccggcggtc gatctgcata aaccggaaaa ggtcattggc aagaacacca ttctctcact
    35941 gcaatccggt ttgatctacg gttttgccgg attggtggaa gggcttagcg cccggataaa
    36001 agcagagctg ggcggcgatg caaaagttgt cgctactggc ggtctcgccc gtcttatggc
    36061 ggacgaagta gattgcattg acgccgttga gccggagttg accctgctcg gtgtgcgcat
    36121 catctatgaa aaaaatcaaa agggctgaca ccctggtttg ctctcaacgg taaagataaa
    36181 gtctccacct tgagggttat gttgcgtttc gtggtttttc agctgttttg gtaaattatc
    36241 gttaaagttc tggtaaaagc aggcgtgatc gcacccaatg aaccgcgcca cctgctgcga
    36301 agacgaaaaa cagggtgatt cgggtgcgcg tttatggacg ctcctgacca gattcgttga
    36361 aatatttact tcgtgaacag aaaaaccgca ggggttgagt gttgctcaac ccctgtggat
    36421 ttgatcagat ttttcgtaca aacctttcaa tgcgttcgag ggcttcctcc aactggttgt
    36481 agccggtggc ataagagcag cgcgcaaatc cttctccacc cataccaaac gcgttccccg
    36541 gtaccatcgc aacacgctgt tcatgcagca aacgggtgca aaaggtgtca tcatccaacc
    36601 cggtaacact gaccttgggg aaggcgtaga aagcgccttt cggttcaaag gttggcaacc
    36661 cgatctgatt gagcccgctg acaattaact tacggcggcg gtcgtactca gccagcattt
    36721 tcagcacatg cggttctcca ctgagcaacc cctcaagagc ggcaaactgg ctcatggtgg
    36781 gcgcagacat caccatgtac tggtgaacgc gcagcaaacc agtgatcagg tgttctggtc
    36841 cagcggcgta accaatacgc cagccggtca tggcgtaatc ttttgaaaaa ccgcccaaca
    36901 gcaagctgcg ctcgcgcatc cctggcagcg cggtgaaaca cacatgtttc acgccgtaaa
    36961 cgaggcggtc gtagatttca tccgagatga ccaccaggtc atgctcctcc gcgatcctgg
    37021 ccacctcgag caggttctca cggctggcta ccgcgccggt agggttattg ggaaagccaa
    37081 tgaggatcgc cttggtgcgc ggagtgatcg ctgcgcgcaa cgcgtcgggg tcaatatcaa
    37141 aattgctttc catgtggctg ggtacttcca ccgccacccc gccggcaagg atgaccgctg
    37201 cctggtagga tacaaagcag ggcgtgggga taatcacttc atcaccaggg ttcaccaggg
    37261 caatagcggc caggttcagg gcttctgacc cgccgacagt aacgatgatt tccttttccg
    37321 ggtcatattc cacgttgtac aaagtactga agtgcgccgc cagcgcccgg cgcagttgga
    37381 taaggccata attcgaagtg tagtgggttt caccgcgctg caatgactcg attcctgcca
    37441 tcaagatggg ttccggtgtt gtaaaatcag gctcaccgat gcccagcgag ataacgtctt
    37501 tcatggtggc ggtgatatca aaaaactttc tgatgcctga cggcttcagg ccggctactc
    37561 tgcgggaaag atatcgatca gtcattgatt ctccttttgt gtgattttgc cgtccgagcc
    37621 agcggcagta acctgccagc cgtcccgatc cgggtcatac accagccagt attcatttcc
    37681 gttctcgcag accaccttga aacgtttatc cagcggggtc tgaatttcat ccagtacttc
    37741 gatgaccttc tgccagcgcc tgccgtccca cacttccaaa gggcgcccag ggtaagcgta
    37801 ttctgcgcgg cagcgcaccg ggtctttcag ctttgcacct gggtgtggtc gcccacaaac
    37861 aggcgcgcgg gttgcccttt gtacacaacc tcccgaccga tgatcgatcc ctcgatgacc
    37921 atgttttcga tcaccgtgtt gtcatccacg atggagttgt tgatgaccac attctttagt
    37981 gtacagcctt tgccaaccga gacatacggg ccaatgacac aggcttcgat tttcgctccc
    38041 ggtgcgatga acaccggcgg tacaatcgta gcacccggta tcccggaatc tggctgtgtt
    38101 gccaccctgc cctgatccag caaatggcgg tttgtctcca acacggcgga aggaataccg
    38161 gcatccaacc aggtgtcgat attttcttta cgcatgcgac cgcccatttg aatgaagatg
    38221 ttgatggcgt ccgccaaaaa atactcgcct ttcaagcttt tcccaaggcg catctgctct
    38281 tcaatcgccc tcaataacgc ggcagtatct ttgaaataat aaaaaccgac cagcacctgg
    38341 ttgttgctgg tatcttgcgg cttttccacc agccgtgtgg cgatgccatt ttttccgatt
    38401 tcgatgacgc cgaaacggcg cggatcttcc actgctttaa caaaagccac tgcgtccatc
    38461 tcttcgaagg gtatgaattg gaggtctgtc tcgatcaatg tatctgaaaa agcgatcaac
    38521 gccggtcctg ccaggtattc cttcgcgcac cataaagcgt gcgactgtcc gcgcatctct
    38581 gcctggatga caaagtgcac cttcttctgc gggtgcgcgt gtcgcatgta ttgctcaatg
    38641 gcatccccct ggttggggct ggtgatgaac acatattcgg cgttttcaac gcccggaaga
    38701 gaagaaaact gttcaagcgc gtaatccaac acggttttac cggccaacgg cagcagcggt
    38761 ttcggacggc tccaggtgag cgggcgcatg cgagaaccat atcccgccat cggtataata
    38821 atcttgaata cctgactcat ggacacctca accgtcatct catcctaact ggtgatcgtg
    38881 tggttacatc atctccattt tacactgcct gcgcaaattg gtcgattgaa tattcacacc
    38941 caacgggcgg ataaagagca taaaaagtcc caccgcgcgc ggtgggagca ggtgaaaagc
    39001 gcctttagta tcttgccagg gtggtgatgg cgggtacctg atcgaacatc caaaaagtca
    39061 aaggaaacgc cagcccaaag gtgaccgcgg tgagcagcca ggccagcagc atccacacca
    39121 ggctgaacca gaaaccaatg accaagaagt acagtgcgcg cagcagaaac gggttttgac
    39181 gtatcgttgt ctgccggatc accggagaac cctccctgat ctccacagtg gtctgcacgc
    39241 gttgcggacg cagggtcatc acctgcggga tacggttgat catgagcagc ccgataggca
    39301 gcccgacaat ggtaaggttg aagaaccagg caacgaccac ccaaatgagc ccgagccaaa
    39361 gaccaatgaa cagaaaatac agaccgcgca ccaggcagcc cggtccccgc tcgatgagtt
    39421 ccactcttgt gttctgctga gtcatgtttg atactctcct tttccattca tatacgcaaa
    39481 taaaacactg ctgttgcagg tcagcagaaa gggagagaga aaattcacag agcgggttca
    39541 gtcgagggcg ggcacctgtt ggtgccagtc tgttacaccc tggtaggcat gcgccgcgcg
    39601 gaagagcaca tcttcacgga aatgcgctcc aaggaactgg ataccaatcg gcaggtggtt
    39661 gtcatcgaac cccaccggta aagccagccc gggaatgcct gccaggttgg taggcaatgt
    39721 gaacacgtcc tccaggtaca tggctagagg atcgccggcg tgttcaccca gtttaaaggc
    39781 agtggatggc gccaccgggc aggcgataag gtccacatgt tcaaaagcct ggtcaaaatc
    39841 gcgcctgatg agcgtgcgca ccttttgcgc ctgaccgtag taggcgtcat aatagccggc
    39901 ggagagcgcg taagtgccca gcatgatgcg ccgcttgact tccgcgccaa aacccacgcc
    39961 gcgcgaccgt ttgaacattt cccatagcgt tgcagctggt tcgcgcagac caaagcgcac
    40021 gctgtcataa cgcgccaggt tggcggaagc ctcggctggg gcgatgaggt aatagaccgg
    40081 cagggcgtac tcggtgtgtg gcaggctcac ctccaccagc tcagcgccaa gcaacctcag
    40141 ggtatcaata gccgcaagca cacaatggcg gacctgtggt tgaatgcctt caataaagta
    40201 ttcctgtggg attcccacgc gcagcccatc cagccgctcc ttttcccgca ggttaagaac
    40261 cggaacgggg acatcaacgg tggtgctgtc cagcgggtca ataccggcca taagggagaa
    40321 taacagcgct gcgtcttccg cgttgcgcgt gaacacgccc atcgaatcca gagaggagcc
    40381 gtaggccacc aacccataac gcgagacgcg cccgtaagtc ggcttgatac cgcagacgcc
    40441 acagaaggat gctggctgcc gaatcgaacc gcctgtatca gagcccagcg cagcaggtac
    40501 catacccgct gccacagcag ctgcgctgcc gccggaagag ccccctggta cacgcgtgac
    40561 atcccaaggg ttgaaggtag aaccaaacgc ggaggtctct gtggaagacc ccatagcgaa
    40621 ctcgtctgtg ttggtcttgc ctactacaat ggccccggcg tctagcaacc gctgcaccgc
    40681 gctggcggtg aagggcggca caaaatcttt gagaatggcc gagccgcagc aacaacgcat
    40741 tcccgcaacc gtgatcaaat ctttgactgc gatgggcagc cccagcaggg gcggggtttc
    40801 gctaccgctg tggcgtgcag acgcgtagcg ggcatccgcc ttatccgcaa ggctgagcgc
    40861 gtcttcctcc gccagggtga cgaaagcgtg aacacgcggc tccaggatcg cgatgcgttc
    40921 caggtaagcc tgtaccagct cgcggcagga aaactccgcc tttttcagtc catccagaat
    40981 ctgtataacg gaaagatcgg tcaagctcgt gttcattcca gcaccggcgg cacgcgaaac
    41041 tggtctttca ttttctcggg agcgtttgcc agcacgcttt caacggtcag ccctgggcgg
    41101 ggttcatcga cgcgcagcct gccgttagca gggagaacgc ttgaagtggg tggtacgcct
    41161 tctgtgtcca gcgcctgcaa ccgcgccacg tgttccagga tggccgaaag ttgctgccgg
    41221 tactgctctt tctcttccgc ggtcaggtca aggcgtgcca gttcggcgat atgttctact
    41281 tcaacaaggg ataatttcat gcgttgatta tacctgagtg ccggaggttc attcgactga
    41341 cccagaggat taatttgcag attgaggaaa aatagcctat actaagtgta cattttttga
    41401 agggataagg aggatagaaa tgccaagcat tatcgatatt gaagggattg gctccgttta
    41461 tcaaaagaaa ttaaaagcca ttggcgttgc cactactgat aaattactgg aactcggcgc
    41521 tacccccaaa ggacgcaagg aactggccga gaagactggc accggggaag cgctcatcct
    41581 tgaatgggtg aaccattccg acctgtaccg catcaaaggt gtgggcagtg agtattctga
    41641 cctgctggaa gaagccggtg tggataccgt agtcgagttg agcaagcgtg tggcgaaaaa
    41701 cctctatgaa aaaatggtgg aagtcaacgc tgccaagaaa ctcgtccgaa aattgcccgt
    41761 tgagaaacag gtggcagact ggatcgaaca ggccaagaaa ttaccacgta aggtgagcta
    41821 ttaaaaaccg tccttcagca cagggtgaga gagtctctca ccctgttttc gtattgaaca
    41881 atctcggcaa agcgctcttt ttttatgaaa ctggtttatc tggcatctga aaacccggta
    41941 aaaatcgctg cggttgagga gggtttcagg cgcgtgttca ccggcgagga gttcagcgtg
    42001 cgctcggtga gcctttcgct caacctgccc gcgcaaccta aaaccgatga tgaaacgctc
    42061 ttctgcgctg aacagcgggc agcgaacgcg cgcgagacgg cgcctgacgg agattattgg
    42121 gtgggtattg agggggggac cagcgaacgc ggcggggaaa tgggcacctt cgcctgggtg
    42181 gtcattcttg attcgttacg cagggggatc ggccgcagcg gtgaattttt tcttccaaaa
    42241 atactggcgg acctggtacg gcagggtatg gagctcggag acgccgatga cctggtcttc
    42301 gcgcggcaaa attccaagca aaaaaatggc gcggtcggtc tgctgaccgc tgacgccatc
    42361 gacaggttgg agctctatat tcccgcggtt atcttcgccc ttatcccctt caaaaacccc
    42421 ctgttatttc cctgatcgtt ctctttttgc gccaatacct gtctgtcatt tttctgattg
    42481 caggcgggtg aatgcttgtg cgtaccagcg caggtaatcc gccacgtgcg cgctccagta
    42541 agaaggttca tgcctgcctt caaactcgaa gaactgatag ggtaccccgg catccttcag
    42601 cgcggtgaca aattcctgta ctttttcttt ttcagcatcc ttatcacccg catccaccag
    42661 caccaagggg atactttccg gcggcaaacc agccgccgcc tgaatattcc ataactcacc
    42721 gtaaaaaaga ggtgtgctat gagcgcccac gataccgaac acttcggggg atacaaaacc
    42781 caggtgcacc gcccaattgc cgccgcgcga aagcccgcca atcgcgcggc aggtacgctc
    42841 tttgcaaacc gcgtaatttt cctcgatata aggcagcacg tcctccatca acgcttcgtc
    42901 aaattgagat tcccgcggtc cgggcgtcca ggtgacctca aagggcatca cgatcaccag
    42961 gggttggatc tctttgctcg tgatcaaatc gtccgccgcg ttggtgagtc ccagctccag
    43021 ccattgtgcg ttggtggctg cctgcccgtg gagcataatc aaccaggggt agccgccctc
    43081 ctttttgggg tcgtaacacg gaggcaggta aatattggtg tttatcgggc ggttgagctt
    43141 ttgcgtggga atatccacgt tcagaatgct gccctgtgtg gctgtgcaga caaggggcgt
    43201 gggtgtgcgg gttgcctgcg gaacaggcgt ggacgtagga tgaggggttg aagtgtcagt
    43261 agcggctact gttggaattg ggctgctgtt cactgccgcg caactgacca gaaaaagaca
    43321 ggtgagcagg ctgatgagtg tggtggcggt gcggggcatt ggtttttcga cgtgctcctc
    43381 ggggctcagt ttgcttgacg ttatccagca atccggatta tactctatgc gctcggggtg
    43441 tagcgcagtt ggcagcgcac cgcgtttggg acgcgggggt cggcggttca agtccgccca
    43501 ccccgaccat caaaccagaa aatctccata ccgctggcag cggtgaacct gttcgttctt
    43561 cttattcctg aaccataacc aaaccagtct gcccgttttt tacctccttg ccattcgctg
    43621 gatgggcgtg ttttgaagga tggaagtaac aatgtatcag cgatgcgggt tttacggggg
    43681 acagtgcctt ttcctattga atcaagccgg cgtctatgag cctgcgcgcc agcatttctt
    43741 ctccttgagc atcaaggtgg atggcgctat tggtgaagtg ttcctgcggc accaggtccc
    43801 acaggtcgaa atatttccag ccgttggctg agctggcttc cgccagggtc tggcggtaca
    43861 ggtcataagc ccagcgcgga taatagaagt tatagcggat atcactgttc tcgccgttgc
    43921 tgactaggat tggttcgttg accagcacga ccggcacctc gccggcgatt ttcatccccg
    43981 cctgtaacag gtccagcgcc agtccgtctt cagggaaggc gggcggctgc cagtggtgga
    44041 agctctcatc tgcttccagg tcacgcccgg cgggcgggta agagggcggg taaagctgat
    44101 cgatccctgt ggcggtccac atcactccgt agagctgcaa gcgcagcaaa tctgccagtt
    44161 cacggcgacg cccgatgaga gtacgctgcc agaatgttgg ctctcctgca ggcatggtga
    44221 tagccaactg gtaacgctca atgagttggc gcacccgttg cgggttatgg gccacgatgg
    44281 gcgattccag ctggcggtcg cgcgggaagg attcaagagt gacccgccac aggatgaggt
    44341 ctggctggta actcatggcc cgctcaagca ccatcaggtc tttcaccaag gagagggtgg
    44401 ggtagcccag gttgtacacc cgcacccgtc tgccatcagc gcttatcaac ccagcggcgt
    44461 ctaattgacc tgccagtgtc tcttctggtt ttaagagcgt gccccaggta gcgctgtctc
    44521 cgatcaccag cacgcgaaat tcatcagccg ctttgggggt accatcgagc ctgtgggcgg
    44581 cgaacatggc ttcaaagttg tacaggctga agttgtatgc ctctctgggc gtctcaccga
    44641 aaggaaagcg ttctcgtccg cgcaacaggg aattgtaagc cgagattctg cccagtccgt
    44701 cggatggtat cagggcgtag gctaaatgga taagcgcgaa aagcagcagc cccttgagca
    44761 gtacattgcg gataaagcgg ggtgtcacag cccgcctcca aacccgaaga gcctgaccag
    44821 cacgcgcgta gataaaccgg gctcaggcag ggtaaaccac acccacccca gggtgacata
    44881 aataaaggtc agcagcgtgt tgagaacatg aatggcgcgg gaaagcccgg ttttggcctg
    44941 ccagcgagcg gccaggtgct gcgttcccgc gctccagcga ttctgcacga acaagcccaa
    45001 cccgtgccag gctccccaca gcaaaaaatt catcgtgatg ccatgccaca aaccaatcag
    45061 caccatggtg ctcaattgcg tcaccaatat caccagccat gccggcatcc acctggcgcg
    45121 gttgcgtaaa aaacgcgtga ccggaaagaa gaagtaagcc cgcatccact gggtgagggt
    45181 gatatgccag ttgtcccaaa agcgcgtgag gttgctcttc aggtaaggcg cgcggaagtt
    45241 ttcaggcagg cggatgccca tgaaacgcgc catgcccagg gcaatatccg tgtatccgct
    45301 gaagtccagg taaatttgca ggctgtaggc cagcagcatc aaccacaacc aacccgcggc
    45361 gtctacctgg gccgcattgc tggcgttgag agagacgata gacagggtat ccgccagcac
    45421 aaacttcttg aacagaccca gcatcaggcg tgtgctgccc gcaccaaaat ccaacgcgcc
    45481 tgggataaat tcaccgcgca ggtctttaat gaaacgctcg ctgcggtcaa tgggtccggc
    45541 gctgatagcg gggaagaaga ggatgtagat caggtactcg cgtagcgcca tttgcggcag
    45601 cctgccagcc tgccggtcgc gcagcgtgtg gatcagccgg aaagcgatgt aagaaaatcc
    45661 cagccagcgc acatccagcg cggatgcaag ctcgcgggat tgaccgctga aaccgcgcaa
    45721 cagtgcgctt gtggtcaacg tcagcgcagg cagtttgagc accaggaaaa gcagcaaaac
    45781 caggatgatc gccagcgtca atgcgggttt gaagcggtgg ctgatgcgcg ccgccatcaa
    45841 caacaacccc aggttcacca gcacaacaat ccccacggtc aacgtctggg gcggacgtga
    45901 agcgctgagc aacccgccgc tgcctaaata acgtgtgagg gcaatgacca gaacggtcgc
    45961 cgtaacgatg cccagcgtca ccaggatttc acggcgctgg cgttcttctg gtaatgaggt
    46021 cagtgcccag cccaatccgg ccagtgagag ggaggcacac ggcagccaga aatccaaccc
    46081 gcggataggc aggatgggtt gcagccagaa gatgatcacc accgagctga ccagcagcaa
    46141 cacaacgcgc caacgccgtg cgcgccggat gctgcaaacc accagcacac caagagagat
    46201 accaatcagc accaggatac ggagcagcgt catttaaaaa ccctggtaga tccacagcgg
    46261 cgcttcgcca gaagagagaa tgaccagcca caccaatatc agcgcgaaag ccagcgccca
    46321 caggttttct tttgagaaaa tacgtttcat gctatgttct ccactgaaaa atcatcctgg
    46381 tgtttactga tcaaccgccg agaaagcgca gcggaaaccc aggtcatcat ccgcgtaata
    46441 cggactgacc caatagcgtt ccgcgctgcg aataagaaag gggctgaaca ggtaagagcc
    46501 accgcgcgca atacggtgcg cgccattttc tggcccgctt gggtcgtagg ttttataagg
    46561 agaggtagtg tactctccat accagtccag tacccattcc atcacgttcc cagccatatc
    46621 cagcgcgcca tacgggctgg cgcctgccgg ataactgccc gtttcagttg tatctccgac
    46681 cagcaggtca tagttggcgt gtagcgtccc gggttctatg ttcccccatg ggtagatgaa
    46741 accttcgctt ccgcgggctg ccttttccca ttccgcctcg ctgggtagtc ttttgcccgc
    46801 ccaccggcag taacgaccgg cgtcgaacca gttcacatac acaaccgggt aatccaggta
    46861 ctgcgggttg ccataatact cttctcgtgt gaaagaactg gagcgtcggg gttcattaca
    46921 gacgccgtct gccacacagg ccctgtacat gcgattggtg acctcggttt ggtctatcca
    46981 gaaggcgtta agttgaactg tatgctgcgg atactcatcc ttttcgcctt cccccttcag
    47041 tgagcccatt aaaaaatcac ctttggggat aaaaagcatg atcgcgccat cgtccaatgc
    47101 catctgcgta cgggcagggg tggggattgg cgtgtcaggc tggtgggtcg ctggcgatag
    47161 gatggttggg ttgctggttg gcgctgcctg cgagctcgaa cggacctcgt cctccacgaa
    47221 aggcgagaag gttgccacac gctccgtcgc cgtgggcgct tcagcgcagc cacaaagcgc
    47281 cagcgtgata atgaccagca gcagacccct gaaagcgctg gtttcgctta cttttgcccg
    47341 cacaccagcc actcgccgcc ctgtttttgc agcagaaacg ttgtgttgga aaggtcaatc
    47401 tcctggtctt ctgtgccata agtggcaatg atcttcccct tgcagttgac agcggcgcta
    47461 ctaccatcgt tctccaattc tgaacagacg atgttgtcta cccttgcctt gaccccggta
    47521 aacgaatcca gcattaactt ggattgctgc tcccaatccg cgcaagaaag ctggctcaat
    47581 gaagacgcgt ctccggcaac cagtgcgtta tagtacgcca ctaccacatc agccggcgaa
    47641 ttggagccgg aacaggatac cagaaacaaa caaaacccaa gcagtagaag tactgttttc
    47701 catcttttca tcatcatatc ctcgtcaaat taagattatt tccagtttgg tgggtgccgc
    47761 ggtcagggtt ccaacaccac acgcacctta tccagcatcc ttaaccaggc gcggttttgt
    47821 tccacccagc cgtcagcagt gaggtacacg ttgttgggcg cctgcaagcc attattgggc
    47881 agtggttgaa ccgccctcca tacgttcacc agcgggatat catagtcggc ggctaccctg
    47941 gcgttcgcca agttgagttt ccaatcctct tcaatattat ccgctttggt ggccaggata
    48001 ggcagggctc cgctggcaag gatggtttcc accactgtgc gaaggtgttc ctcaaaggtg
    48061 atctcctgcc tgggtttcca gttcgtgccc agggctacca gcaccaccac cggtcggcgc
    48121 gtgcgcaact cacaatccaa gggtgtttca gttcgctggc attcctgata tcccgcacca
    48181 gcggcaaaca tcgggttcaa cacactgctg acccccaatc catttgcagc ggtgatcgaa
    48241 tccctgacca tgctttcttt gaaataatga gcggtgcgtt catactcttc ggggagaggg
    48301 taatctccct tcacataacc tgccaggaag gtcgccgggg tgaactggca gtcacctatt
    48361 ttggaaatcg aacggaggtt aagcgaggtg ttcgtggcgg cggatgcgag gatttgaaca
    48421 gcgtgctcag atagttccgg cacgaccggc cactcccgcc agcgctcagg tgaacgctct
    48481 gtgttctgcg cctgagaaaa agaatacacc gcttctggag cgcttcccgc gcctgttgtt
    48541 agcaaggcta caaatagaag aatcaacaac aaccggcttc gcatgatgaa ttctcgcgtg
    48601 tggaggatga actgcctgcc tggcacaccc caaattataa aggacaaccg gtttcttcac
    48661 acccgcgaaa agacggctaa aagcgtataa taacaacaaa tggcacccaa aacacctttc
    48721 ctctccattg tttttcctgc gtacaatgaa gaactgcgcc tgttggaaac gctgcagcgc
    48781 acgctggcgt ttattcacac actaccctat cccgctgaga tcgttgttgc cgaaaacggc
    48841 agtgtggatg gcacgtttga gatcgcccgc tcgctttccg ttttacatcc tgaaatcacc
    48901 accctgcacc ttgaaaaagc cggtaaaggg cgcgcggtgc aagctggcat gctggcagcc
    48961 aggggacgtt accgcttcat ctgtgatgtc gacctatcca tgccgattga agaggtgccg
    49021 cgttttctcc cgccacagat cgaaggtgag gatatcgtca tcgcctcgcg tgaagccccg
    49081 ggagccatgc gctatgatga gccgtggttc cggcatttca tcggccgcat cttcaacaac
    49141 atggtgcgct ggatggtgct gcccaagctg caggataccc agtgcggctt taaatgcttc
    49201 agcgacgaag cggcagaagc gctcttcccg ctgatgacca taccgggatg gacttttgat
    49261 gttgaggtgc tggcaatcgc ctttaaaatg ggtttccgcg ttaaagaact tccaattccc
    49321 tggtactacc atccgcacag caaagttcgc gtggtgcgtg attcagtgcg catggcaagc
    49381 gacctgctca agattcgtag aaatttacgc cgcggcgagt atgaccttca gggttgaccc
    49441 ggggctcatc cccgccgcgc ctgacctggc gtttgaattc gccctttggg aaaatgggct
    49501 ggaatctgta gccgggatcg atgaagcagg gcgcggcgcc tgggctgggc cggtgacagc
    49561 tgccgtggtg gtctttcccg cttattttgc cctgccgcaa aacctggttt tcattcgcga
    49621 ctctaaacaa ttgagcgcag aaaaaagaac agccctcgaa cccctcatta aacaaaaggc
    49681 gatggcctgg tcagtcggtt gggcttcgaa ccaagagatc gattgtttgg gcatccttcc
    49741 agccactcgc ctggcaatga cccgcgcgtt ggaactgctc aagttaaccc ctcaacacct
    49801 gttgatcgac gcgttgttta ttccggaata cccctgtggt cagactgctc tcctgaaagg
    49861 agaccagcgt gccctgagca tcgcggctgc ctccatcctt gcgaaaaccg ctcgagataa
    49921 atttatggga gagatcgcca gcaagatttc gggctacgcg ttcgaacgca acaagggata
    49981 cggcacccgg gcgcatcaag cggcgctttc cagccatggt ccctgctcag cgcatcggtt
    50041 cagttatgcc ccggtgaaaa gaagcctgaa aaaacaacag gtggagggtt gacctccacc
    50101 ttgcttatct tctcatcaca atcaagccag gcgggtcttc tttccgtgcg tgtacgaaaa
    50161 ccatttgacc agttccgcta caaccgaggg cacaaccagc aatggtagaa gcagttgcca
    50221 ttgctgccag gcaaggggga ctgttttaaa aatggggttc aggaagggaa catagacgac
    50281 taccagcacc aggagcaatg aaaacagcac cgcgatattc atccatttgt tggaaaaaat
    50341 accctgcttc agcaacgggt aacgctctga acgggcggtg aaggcccgca acaactcaga
    50401 agacgaaagc gttacaaagg ccatggtccg ggcaatatca agattgttct ctggcgagag
    50461 cccttgatag ttgaggcctg taaagaaagc ggttagggtc gcggctgaaa tagcgatagt
    50521 ctgggttgtt acgccaagaa gcatttgccg gttgatgatg ggctctttgg gcgccctggg
    50581 aggttgatcc atgatgtccg gatcaccttt ctctgtactt aaagccagcg ctggtgcgcc
    50641 atcggtaacc aggttcagcc agagcaactg aatgggcgcc agcggcgcaa gctgctcagt
    50701 ggtcagttga gggaacaaca gaaagcccaa cgccgtaggt aaaaagataa taaggatttc
    50761 cgccatgttg caggaaataa gaaaatacac aaatttacgg atgttggagt aaatgattcg
    50821 accttgctct accgccgaaa cgatactgac gtaattatca tcggtgagca ccatatccgc
    50881 ggtgtctttc gccacatccg tgcctgtaat gcccatggaa acaccaatat ctgcctgctt
    50941 gatagcgggt gcgtcgttca ccccgtctcc agtcatcgcc acgacctcgc cgttcgcctg
    51001 cagcgcattg acaatgcgca gtttatgctc aggtgagacg cgcgcgaaca catccgtcct
    51061 cctgacttct tctttcagtt catcgtcgct catcgcttca agctgcgcgc cggtgagcac
    51121 atggcgccct ttccccagca agccaatgct ttcagcaatc gcgcgcgccg tgttgggata
    51181 gtctcccgta atcatgatgg tacggatgcc ggcgcgcgcg gcagtttgca gggcagggtt
    51241 cacttctggc ctggcagggt cgatcatgcc gataagccca ataaacacca ggtctttttc
    51301 taattcttcc cgctcaacct gactgggcat ttcgtttacc atgcgaaagg cgacgccaag
    51361 cacgcgcagc gcatttcccg tcatctcatc attggccgcc agaatctgct gtttggtgtc
    51421 ctcatccaaa accgcctgaa caccattatc gatgggttga taatgcgtgc acaggttcag
    51481 caccacatcc ggtgcgcctt tgacggcgac cacaatgctg cccctgccct tttcttcgct
    51541 atacggtgat atgtcgttta ggcgcgggtc atccactgtg tgaaccgtca ccatgcgttt
    51601 gaaatccgag tcaaatggta tttcattggt cctgggatag gccttattca acgcggcgat
    51661 ctccccaccg gccttgtaag ccgccaccag tatcgagcct tcagtagggt cgccaaccat
    51721 gcggaattct ttttcaccat cttctctttc ggtttcttca agcacggcat cattgtttag
    51781 cgcgcctacc cacaaagcgg tacgaacccc ggcgtactgg tccagatgga tctcgcgctc
    51841 tccgatggta aacgagccta tcggtgtgta accctgccca gtcactttta ccatgtgtcc
    51901 atccacccaa atatgggtaa cggtcatctc gttctgggtc agcgtgccag ttttatctga
    51961 gcaaatgact gtggctgagc ccagggtttc gaccgaagac aggcgtcgga tgagcgcgtg
    52021 gcgtttgatc atttcctgca tacccagagc caaactcacc gtcaccaccg cgggcagtcc
    52081 ttcaggcacc gccgccactg ccaggctgac cgcgatcatg aagagaccca caatcaggtc
    52141 cgcgctgatc gcgccaatgc tttcgacaag ccctactaca aaaaccagcc cacaaacaac
    52201 cagggctgcc aagcctagtg tctttcctaa ctggtccagt tttctttgca ggggggtttg
    52261 ttcctcttgc acagattgca gcatgcgggc gatgattccc agttgggtgt gcatgcctgt
    52321 gctcgtcaca acgcctttgc cgcgcccata agaaaccact gtgcccatga aggcggtatt
    52381 ctttcgatcg cctaaagaag aaagagggtc aagcgtcaaa gctgcgttct tttgcacggg
    52441 cacagattct ccggtaagcg gggcttcatc gatgcgcatg ttcaccgctt ccaacagccg
    52501 cacatccgcg ggcacgtagt aacccgcttc cataaaaacg atatcccccg gaacaaggtg
    52561 cgaagagggg acgctgatgc gttttccatc ccgcaacacc tggctttcag gcgctgccat
    52621 gcgtcgaagc gcggccaggg cttcctctgc c
//
LOCUS       DAOTLW010000008        51596 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_786_length_51596_cov_10.701480, whole genome shotgun sequence.
ACCESSION   DAOTLW010000008 DAOTLW010000000
VERSION     DAOTLW010000008.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 51596)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 51596)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51596
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_786_length_51596_cov_10.701480"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            118..1296
                     /locus_tag="P5516_02350"
     CDS             118..1296
                     /locus_tag="P5516_02350"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HRT91311.1"
                     /translation="MITRIKQGMLIFLVACLLISCTNTAKISASDKVNTSVAQTVAAA
                     QPTGGLPLPDGAVQPTNPAPPPVEQPTTTPPPLPTLAPSIPTLPPSPTTDTCNQAKFV
                     QDVTIPDGTKITPGLAFTKTWRIQNTGTCSWNTAYAVVFDSGHSSGAPTVTPLPANVP
                     PGGTIDVSVVITAPTTNGDYTWKFMLRSDTGRIFGFGAGYAYPMTAVIKVEPLILLTA
                     LPHFTLIPIVFPKYDFTANYCSATWSNGHQDLPCPGTKNDSTGFVVQQDKPRLQDGNT
                     RDTKSLLTHPEWVDGGAIVGIFPAVSIESGNRFKTTIGCGYSGTSCDVLFRVNYKKGS
                     DPFVELGTWSMKYSNAPLDIDIDLSSLAGSDVKFQFIVFANGSSSQDWAHWVFPRIAK
                     "
     gene            1369..2760
                     /locus_tag="P5516_02355"
     CDS             1369..2760
                     /locus_tag="P5516_02355"
                     /inference="COORDINATES: protein motif:HMM:NF019142.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like domain-containing protein"
                     /protein_id="HRT91312.1"
                     /translation="MKRKHWPMLMIAVPIIGLVVGYLLMTRNVPATIDGTPLTIRTHA
                     LTVGGALRSAGFAVDEGDAVFPAAGTWLSKADEIRLDHARLVRVYDTTQKQPIELSTS
                     ALTAAEVFKQAGLTLGSLDSLTLNGLPVGPNEKLPFGREMTLNYRPAVQVRLNVDGRD
                     TTITSSAGTLGEALWQAGFRLREGDRVTPGMDTALTENLAVEIHTARPLSISVDGTIL
                     EGYSAAPTVAAALAENGVTLQDIDTSQPGAGDPLPEDGVINVTRVTEEIHAEQRVIPF
                     EIESLADDTMFINETNITQAGQNGLAATLVKVRYEDGLEVSRQTQNEVILIPPVAQVE
                     YRGTKMLENYIDTPEGALQYYLVIDAVATSFSPCRQGIEGYCSNGTASGRTIQKGVVA
                     FKRNWFNLFRDTRVYVPGYGIGVVGDTGSYPYSDSWIDLGYSDADYVPWGAVKVKIYF
                     LSPPPSGFTGVLP"
     gene            2790..3626
                     /gene="rsmA"
                     /locus_tag="P5516_02360"
     CDS             2790..3626
                     /gene="rsmA"
                     /locus_tag="P5516_02360"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HRT91313.1"
                     /translation="MKRHHLDPRKGLGQNFLVDHHALRRIVKSAELTGGEEVLEIGAG
                     LGSLTRLLAAAAGVVRAVEIDHRLMPVLREVLSSYANVELVQGDILALDPARLMNEPG
                     YIAVANIPYYITSAIVRHLLECPKRPARLVLTMQKEVAERICAEPGDLSLLALSVQVF
                     GQPSISAVIPAGAFYPVPGVDSATLRVDLHPQPYFTDEQRKMFFSLIHMGFAQKRKTL
                     RNNLAAGLHLSGEEAARYLVDADIDPQRRAQTLSLVEWKTLVNELKDLLGAKREQETP
                     PA"
     gene            complement(3623..4357)
                     /locus_tag="P5516_02365"
     CDS             complement(3623..4357)
                     /locus_tag="P5516_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HRT91314.1"
                     /translation="MSICVIIPTYNEVENLPLIVARLRALPLEDLSILVIDDNSPDGT
                     GALADELAERYKGQISVMHRKGKLGLGTAYISGFKQVLQTNTAYIAHLDADFSHQPEK
                     LLEFMKSIEGSDVVFGSRYTPGGSLDRHWPAWRKWLSNFGNTYARTILRIPTKDLTGG
                     FRLWRRETLAAMPWDRVRSNGYVFMVETAYLAHRLGFKIKEVPIYFAERTRGKSKMNF
                     SIQVEAALRVWSLISRYRDIQPLPRG"
     gene            complement(4463..4855)
                     /locus_tag="P5516_02370"
     CDS             complement(4463..4855)
                     /locus_tag="P5516_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HRT91315.1"
                     /translation="MELIDRQALIDRIRVLQHSLPEVQAAAVISLDGLIMASALPEGM
                     SEDRISAMSAAMLSLGEQINKETGLGTLEQLYTRGSNGYVVLLAVGKEAVFTTLVQPE
                     AKLGILFLELRKTAEDLARLLNGNGLNT"
     gene            complement(4946..5845)
                     /locus_tag="P5516_02375"
     CDS             complement(4946..5845)
                     /locus_tag="P5516_02375"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRT91316.1"
                     /translation="MNQDTEKINLRERLVSDTYLLLAAIIWGAGFVAQRMASFHLGFN
                     AFNGIRFLLGGLVLLLFVWRRFRAFKGGWGWVLLAGGVLFSASSLQQIGIGTTSASTA
                     GFITGTYVVLVPVLLALFWRQRTPVVTWVAALVALAGTYLLSTGGTVLIPSTGSLILL
                     AGSVVWALHVIVVGLAVKKIDVLVFSVGQFLVCGLIHLVMSMFIEPVSMPALRASWLP
                     LLYSSLFSVVLGFTLQAIGQKKAPSADAALLLSLEAVFAAITGVLFLNEQLNLVQVIG
                     CVVILGAILFAQLIVLKQNRKPL"
     gene            complement(5918..6193)
                     /locus_tag="P5516_02380"
     CDS             complement(5918..6193)
                     /locus_tag="P5516_02380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91317.1"
                     /translation="MAEKTQAEKHQIIHLLQQIPFDETEKALWMENVEENGVTEELLD
                     EMHTKLLALPQEKFASEWVKAKLSTDLARVARQWRMQRASRQFKHNR"
     gene            6320..6517
                     /locus_tag="P5516_02385"
     CDS             6320..6517
                     /locus_tag="P5516_02385"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRT91318.1"
                     /translation="MDNNTKKLYRSRVDRKIGGVCGGLGEYFGIDPTLVRLLFVLGLL
                     FVGGTLLAYLILLIVIPEEPL"
     gene            complement(6593..7819)
                     /locus_tag="P5516_02390"
     CDS             complement(6593..7819)
                     /locus_tag="P5516_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91319.1"
                     /translation="MNLWQAIVEALESLYSNKLRSFLTILGIVIGVGAVIAMMAVGAG
                     AQETITGSISGIGSNLLFVIRGNPTVELTRIEPLTSEDAEAFLDQFQAPSVAGVAPVV
                     NGSLVVSTAGESTRTTVYGVTPAYQQIRNYGLTEGVFISEEQLLGRSSVALIGPDTAE
                     KLFGRREGLVGETLRIEGQPFRVIGLLETKGGSSFGSQDDLLIIPLSTAQARLIRHPD
                     NRLDMILVQVISPELTTQATEEIAQILRSRHRTRIGQDDFTIFSQDDFVDLAKTITGV
                     LTIFLGGIAGISLLVGGIGIMNIMLVSVTERTREIGLRKALGARKKDILIQFLTESSL
                     LSLFGGIIGIGLGWLISYVVGRIAVANNTPFTPQVSLDAILLATLFSTAVGLFFGLYP
                     ANRAANLEPVEALRHE"
     gene            complement(7816..8496)
                     /locus_tag="P5516_02395"
     CDS             complement(7816..8496)
                     /locus_tag="P5516_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91320.1"
                     /translation="MEPVIQTHELTKIYQLGEVSVHALAGVSITIDRGEIVAIMGPSG
                     SGKSTLMNLLGCLDRPSSGTYTLDGEEVAQLDDDHLANIRNRKVGFVFQSFNLLPRQS
                     AIENVELPMRYAGVTKEKRERAIAALESVGLADRMHHRPPELSGGQQQRVAIARALVN
                     NPAILMADEPTGNLDSRSGQEIMELLLKLNQNSGTTLIIVTHDQGVASNTQRVIRLKD
                     GLVEGDTP"
     gene            complement(8506..9927)
                     /locus_tag="P5516_02400"
     CDS             complement(8506..9927)
                     /locus_tag="P5516_02400"
                     /inference="COORDINATES: protein motif:HMM:NF012739.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRT91321.1"
                     /translation="MKKRLTKRTILFILLGLAAASVITYLMLNTLSARKAATAELQTV
                     EVTRGELVAIIGATGTVRANQSAVLVWQTSGRVGDIHVKTGEKVAGETILAELIESSL
                     PQNIILAQADLVEAQRALDNLLDSNLSQARAQLNLANAKDAYERAVWSSWHGDTARTT
                     NQNKIDAAQAAVTLARDRVNTAEENYDKVDERDDDDPLKANALSTLANARESLDKAIK
                     DLDYLVQNPNEKEIAISEGKVAVALAEYEDAQREWERLKDGADPNDIAAAKARIAAIK
                     ATMALAHISAPFGGTVTEVNIMPGDLVTSGGVAFRLDDLSHLLVDVQVPEVDINRVVV
                     GQPVELTFDAINNQAYQGKVNRVARVGTIESGLVNFKVTVEILNPDEQVLPGMTAGVN
                     IIVSQLEDVLTVPNRAIRLVNNHPVVYILKNNIPTEVSITIGASSDSMSEILSGDLKE
                     GDKVILNPPSSFMDVHFSGRPPF"
     gene            9988..11232
                     /locus_tag="P5516_02405"
     CDS             9988..11232
                     /locus_tag="P5516_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91322.1"
                     /translation="MTWRKYTGIAVPLLIMMVLLCGSVVSLANPLDQARLFTADIEYD
                     YIAWLANASGQKASQALLNPVQRLPQEQQTRLVKRYFTLVQALEVVQARVELVYADPA
                     ISDAAAAAAELIRQQHALQQSLDGLSPLVEAILQFQVASVMADEGIGFLAQPLPPVLF
                     HSSPLPKALIVSPRDSIRQEVNISLLADLGLEDITRLEENVSSYMNASALVVDVGGIG
                     VYPTMVQRSSNIEWTLDTIAHEWTHNYLTLHPLGWNYDTTAELRTMNETAASIVGKEI
                     SREVMRRYYADDDTGENKTPKAWLKRTAVNEFDFRQEMHKTRVRVDELLAQGKVEEAE
                     EYMEQRRVVFVEHGYMIRKLNQAYFAFHGAYADMPGGAAGEDPVGPAVRALREQAGSL
                     ALFLRRIAWMNSFTDLQKALAH"
     gene            complement(11271..11636)
                     /locus_tag="P5516_02410"
     CDS             complement(11271..11636)
                     /locus_tag="P5516_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT91323.1"
                     /translation="MPIYDFRCSSCKQRFEVFLTYAEYDHHQTACPSCGSTDLQRVIR
                     KVRVARGDAGRLESLANDSDLDMIDHDPKALGRMMREMKNEVGADDLPGEFDEVVSRL
                     EKGQTPEEIERDLPELGEE"
     gene            11720..12343
                     /gene="gmk"
                     /locus_tag="P5516_02415"
     CDS             11720..12343
                     /gene="gmk"
                     /locus_tag="P5516_02415"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HRT91324.1"
                     /translation="MSSEEVSFDLIHPQPLFIVISGTSGVGKDSVIRGLKQRDLPLHV
                     VITATTRAPRAGEVDGKDYFFFTPDEFKTRQAEGWFAEHAVVYSDLKGIPRHQIEEAL
                     ASGKDVVARVDVQGARTLRKQYPQAVLIFIVPGSFDEWYQRLTRRGTESADDLRLRVA
                     KAREEVKQIGLFDYVVVNPDGQIEKAVDDVVSIIKTEHMAVKHRRIL"
     gene            12343..13119
                     /locus_tag="P5516_02420"
     CDS             12343..13119
                     /locus_tag="P5516_02420"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRT91325.1"
                     /translation="MERFAGSQEEQQPQPPPVGTPVTVRLPVRKPWVTYGLIGITIAV
                     FLLQLLSQSTSHDGVDWPFLLGGKINAYILRGELWRLVTPALLHGSLLHIAFNMYALF
                     SLGSSLERYYGHGRFLVLYIIGSFCGNSLSFLLSPNPSLGASTAVFGLVAAEGVFIYR
                     NRRLFGSRARAMLSNLVLIVVVNLLIGLQPGIDNWGHLGGLAGGALFAWMAGPLLLVQ
                     QTSTGYELQDSRLSGGLLWGILLSAGLFSAMVISRFITGD"
     gene            complement(13188..14087)
                     /locus_tag="P5516_02425"
     CDS             complement(13188..14087)
                     /locus_tag="P5516_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91326.1"
                     /translation="MNKKAFISLMMIALALTLLVSACGNSKGAATTEPPQVIIITATP
                     ESPQVIIITATSEEMAPPIVATEPPAAPPTEPPDAATRPTPPFGYEPTPFIAEDSAQG
                     ERYFLDEFDNGLDQYDHFIYDITVKVNKIIKENEELEKKIKVELEDGTIEFNIERYYL
                     NYYMIYRPQVYEDVKISVEVENLGFPASQAALVCRYDEELGWYELIVSNTGRWRLYYF
                     DKPLVKWNFLSDGGAEKFHYGKDTNKYTLECKGKELSVYINDVFSQTVTNKNLKKGKV
                     GFSIQAPKSWTTMNVPWFEVSEP"
     gene            complement(14215..14997)
                     /locus_tag="P5516_02430"
     CDS             complement(14215..14997)
                     /locus_tag="P5516_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91327.1"
                     /translation="MKIKKWIFVVAITALVSVAGSACAPAAAIESTPQPTQTPYVIVV
                     TPTAAEVSATPEPTEPLAAPDAETPRGEEPPKYFREEFDGGLDDFEQILWTGRFGKDK
                     IYTEDGKLKVRLNAMYLAPYLTYDPYTYTDVRLEVSAENLAYNDNHITLVCRYDEELG
                     WYEFNISSNGLFRINYYDNTLVKGHVKLWNGGSNDIKMGRQINKITAECIGTQLSLYV
                     NDVLLKTVEHKDLREGRVGLGVSSFNQFPVNVDFEYFDISQP"
     gene            complement(15091..16260)
                     /locus_tag="P5516_02435"
     CDS             complement(15091..16260)
                     /locus_tag="P5516_02435"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRT91328.1"
                     /translation="MKPRLSLAVLILTAVFAALFARVQGVSALTSPGKSEADSALCLP
                     ETSEELMNGCLKAGPAARLEELAARGIPPQGRQLFASRTPYELGATPFSYAKLVDDQE
                     IPLYATLADVESGKPSNVLPKSRTKYVALMNSAETSRGRFYQIATTEWVSVELAKKVA
                     ATNFQGYILKESPDFSFGWIINETTSRAGPGYEYPETGVNYWRLDPVMVYDSAVVNET
                     EWVMIGYSEWVEHRVISRVIPNYQRPAGVPVDKWIEINLYENTLTAYENGQMVFATLI
                     ASGVNPFFTQPGVFQIYKKLESDPMSGSFEVDRSDYYYLEEVPYIMYYDKARAIHGAY
                     WQPFLGYQNSHGCVNLSVADAHWMFNWANDGDYVYVWDPSGKIPTDPSFYGDGGF"
     gene            complement(16363..16977)
                     /locus_tag="P5516_02440"
     CDS             complement(16363..16977)
                     /locus_tag="P5516_02440"
                     /inference="COORDINATES: protein motif:HMM:NF022751.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3109 family protein"
                     /protein_id="HRT91329.1"
                     /translation="MDATTKKALSRVNPRLLQSEAIQRCSLADCKGTCCVFGVWVDLR
                     EVDDIMRNAAIILPHMPPDCRVPGEWFAAVEDRDKRSPSSRVIHTAVENRAEHYQGTA
                     CVFCLADGKCALQVAALANNLHPWRFKPFYCVLHPLDLDEEGRITLDAAEDMVNEQGS
                     CLRRAPKPIPLLDTFEPELKYLLGEQTFSCLKQFTSRNTEDEPE"
     gene            17100..18041
                     /locus_tag="P5516_02445"
     CDS             17100..18041
                     /locus_tag="P5516_02445"
                     /inference="COORDINATES: protein motif:HMM:NF012607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HRT91330.1"
                     /translation="MAAWKVLLTDGLEENGKEILRSAAEVTDQSGISAEDLLKVVGQY
                     DALIVRGRTKVTESVLAAAPHLKVVGRAGVGVDNIDLAAAKAHQVTVVNSPLATTVAV
                     AELTLSLMLSLVRELPRADASMKAGKWLKKEFEGRELFGKTLGVIGFGRIGSAVAARA
                     KAFEMKILAYDPLVSAEEIKKRGGEPVSLDELLTAADMITMHMPLTADSRNLLNAEAL
                     AKTKAGVYIVCAARGGVIDEEALLAALNSGHVAGAALDVFANEPPGATELVAHPHVID
                     SPHIGAQTVEAQARAANDIAEEILNALAGKPLRWKVA"
     gene            18056..19561
                     /locus_tag="P5516_02450"
     CDS             18056..19561
                     /locus_tag="P5516_02450"
                     /EC_number="3.4.17.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HRT91331.1"
                     /translation="MMQKKVERFKRMIMEVTDLGHAEAVLGWDQQVYMPRGGGEDRGD
                     ILETIASLAHQKFTCNEMGELLAELKPYAETLDADSDDACLIQRVAHRYDKETRVPTE
                     WVVDFTRVTTLAQMAWEEARAKSDFTIFQPHLEKVVDLRRAYASFFQPYDHVYDPLLD
                     GFEPGLKTAEVQQIFDALRPQQVALLKEIAAKPEIEDAFMHVPYDEQGQWDFGVEVIT
                     RFGYDWNRGRQDKSVHPFTTSFGINDVRITTRFDPSRAASALFSTMHEAGHAMYEQGF
                     SASLRRLPLADGASMAVHESQSRMWENLVGRSKAFWKFFYPRLVQKFPQQLKGVSLEQ
                     YYRGINKVEPSLIRVEADEATYNLHIMLRLELEIGLMDGSVRVADLPGLWNKKMQEYL
                     GVTPPNDRLGVLQDVHWSGGMIGYFPTYALGNLVSAQLWEKIMENIPDLEDQISRGKF
                     DDLLAWLRTHIHRHGAKYEPQQLIKKVTGTGIRPDPYMRYLRAKYAEIYNL"
     gene            complement(19858..20253)
                     /locus_tag="P5516_02455"
     CDS             complement(19858..20253)
                     /locus_tag="P5516_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91332.1"
                     /translation="MATGEERLKVLKMVQEGKITAEAGVELLKALDAASKKAEVDERA
                     AVNFPGKGGGKFFRVRVTDTDTGRTRVNIRLPLGMVNAGLRMGMRFSPEVEGLDAARL
                     VEALASGETGKIVDVLDDEDGEHVEVYIE"
     gene            complement(20253..20681)
                     /locus_tag="P5516_02460"
     CDS             complement(20253..20681)
                     /locus_tag="P5516_02460"
                     /inference="COORDINATES: protein motif:HMM:NF021388.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HRT91333.1"
                     /translation="MINKTLDQCPVCESELMITRLYCVNCDTTIEGHFAAEHTPFGNL
                     TPEQMQFLLTFVKCEGRLNRMEDELNLSYPTLRSRLTEIVRALGYEPGKDEGPAQLSP
                     EGRRDILEKLDRGEISWEEARAILRGEKFIPQHNAAEGGR"
     gene            complement(20885..21250)
                     /locus_tag="P5516_02465"
     CDS             complement(20885..21250)
                     /locus_tag="P5516_02465"
                     /inference="COORDINATES: protein motif:HMM:NF039800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6504 family protein"
                     /protein_id="HRT91334.1"
                     /translation="MKTRPAQFIDEPIQVEFDSPPTFDKKPPCPQRFRWRGQVFTITS
                     VVEERVDYARRGRMSRNMAPAHLASASRSGSWGVGRFYFCVMVDSGQIFDLYYDRAPK
                     DADNRMGGWFLKGERLSQS"
     gene            complement(21308..22225)
                     /locus_tag="P5516_02470"
     CDS             complement(21308..22225)
                     /locus_tag="P5516_02470"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRT91335.1"
                     /translation="MRWVRETVPQPDQNRYYRIAMSITLGGNLLLAVGKGVAAWLSGS
                     SAVYSDAANSVSDVVYSLLMVLGLWVATRPPDLSHPQGHSRFEPLVALMVAAAMALAG
                     IEAARTAYVRYTEPGLSAIRLDLPTWVLLASALVKAAMYASITRISKRLHSPALATSA
                     RDNLSDVLSSLAAFVGVLGSNYIHPLADPVAGFLVAVWILRNAFLAGRENLGFLTGKG
                     ASQEKINQFVTAAESVPGVIKVHHIVTDYVGPRLMLDLHINVDGNSTVNHAHEISDEV
                     TKRLESFPEVDRAYVHIEPHDWEDDKSPT"
     gene            complement(22394..23332)
                     /locus_tag="P5516_02475"
     CDS             complement(22394..23332)
                     /locus_tag="P5516_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HRT91336.1"
                     /translation="MNTKTESKTQQRFIISIILTGLILVAEIIGGLLSGSLALLSDAA
                     HVFSDIFALVLSYFALRLVARPPDERHTYGWHRAEVIAALINGASLLVIAVGIWVEAV
                     KRWQAPLEIKSTEMMIIAVIGLVVNIIVAVILGGHDHTHEHGHDHEGHAEKREKRNLN
                     VHSAYLHVLGDLISSIGVIIAALLIRLTGERWIDPLISVLIGAIILVSAYRLLRKSLH
                     ILIEGVPEGLSLQQLHQSIASVGEVDTVHDLHVWNLGSNQVALSAHIVLSAEFFSQQP
                     LVLEKIKSLLAAEYDIHHTTLQFEETRCGEGHGGCN"
     gene            complement(23354..24118)
                     /locus_tag="P5516_02480"
     CDS             complement(23354..24118)
                     /locus_tag="P5516_02480"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRT91337.1"
                     /translation="MKKNYLIDMDGVLVTGKTPLPGAVEFIDRLLACKVKFLILTNNP
                     LYTQRDLEHRLNTIGLNVPAERIFTSAIATARFLQSQKPNGKAFVIGESGLTQALHDV
                     GYVQTDLSPDYVVLGEVTNYNFELITKAVRLINDGAMFIATNPDSTGPLEAGIVPATG
                     ALAALIQKATGREPFFVGKPNPLMMRSALRYLGVHSEDTVMIGDRMDTDIVAGVMSGL
                     ETILVLSGVSTREDVERFPYRPTQILNSVGEIKIAC"
     gene            24248..25144
                     /locus_tag="P5516_02485"
     CDS             24248..25144
                     /locus_tag="P5516_02485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class D sortase"
                     /protein_id="HRT91338.1"
                     /translation="MPRRRAIEDYTASELRQLLMEKSRVEREARLEAFRKAGRLIEAP
                     LPAEGETPEPAIEAEEYTASRARRTARRRRTGLDRFLFAVEIIAIIGLLFIIFNGVKL
                     MRELNQQVASVIEQPTLTPTPLITAVVLPGGHVPPVSGQENRFNEAEIPEHLRPLMQS
                     YANLPVPTQSPQQARQIQIPAIGVSAPVVLGDGWEQLKKGVGQHIGTANPGDKGNLVL
                     SAHNDIFSEIFRDLDKLKKGDEVTIITQDRSFTYVITSSQIVEPTRVDLLAQTSDSMI
                     TLISCYPYLVDNKRIVVQGILQ"
     gene            25171..25383
                     /locus_tag="P5516_02490"
     CDS             25171..25383
                     /locus_tag="P5516_02490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91339.1"
                     /translation="MAKKVKKRSTNAPAAVVAKTETATPKAAASRTPTAEFDPDYSFV
                     KKDLKRIGILAGSFFVVLVVLSFFLR"
     gene            25444..26514
                     /locus_tag="P5516_02495"
     CDS             25444..26514
                     /locus_tag="P5516_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012488.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HRT91340.1"
                     /translation="MTRMFVPGPVDVAPEVLEAQTKPMLPHRSKDFEAIFRGTEEKAR
                     QLFYTQFRVYQAASSGSGMQEAGIRNFVQKDVLSCINGAFAQRWYDVAVANGKTADKL
                     EVEYGEAITPELLRDALKQKHYEAVTIVHNETSTGVENPLADLCAVVKEISPDTLILV
                     DAVSSLGGVKIEMDKLGVDFLLTSSQKCLALPPGLALAAATDRALAKAENVSNRGWYF
                     DLLLMEKHRVKDSTPMTPAMALIFALDRQLDRILAEGLDARFARHAAMARRVQEWASG
                     HGMEPLAKPQYRSKTVVTIKNSLNWDISALNKFLQTRGMRIANGYGKIKDLTYRVATM
                     GETSMADIEALLVAMEDYMKQS"
     gene            26653..26724
                     /locus_tag="P5516_02500"
     tRNA            26653..26724
                     /locus_tag="P5516_02500"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:26685..26687,aa:Lys,seq:ttt)
     assembly_gap    26727..26826
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26827..27392)
                     /locus_tag="P5516_02505"
     CDS             complement(<26827..27392)
                     /locus_tag="P5516_02505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91341.1"
                     /translation="MRASLGDESARVWQEPNSESISLTSIHKGEEFEIGKVIRKKKEV
                     WVTVTLDNGVTGYISGDTHIFAIQQVEAVGNDLELYQQPDDSSPVLATITKKTLFTVR
                     GVETVNEESWYRAETGEGVQGYIKSGARLRAKPQVTKESARKMMITGALFAVGGAVLY
                     FLFPSNPEAAGGNTSFISLGLILLGLFQV"
     gene            complement(27395..28084)
                     /locus_tag="P5516_02510"
     CDS             complement(27395..28084)
                     /locus_tag="P5516_02510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91342.1"
                     /translation="MPLKIMVPLERVQQALNSSLVLAFPKRCSRCGAVPAEDYETHSL
                     RLRIGRKRPGLYRQTYKEDRPYRLKIRVCQTCYRADFATSVEEMEKDDTSAGRLARIY
                     SRLYTVGGVVACAGMLLMTRFIPADSALGGVKAYWPYIVGLGGAIILAVWLHQRYRTR
                     KLIEELESAGVSLDARPRAKIYTPVPEDKSDPSAIVLEINLHDDDWAAECAAYYHFQT
                     AEYTPGVFQGE"
     gene            complement(28336..28737)
                     /locus_tag="P5516_02515"
     CDS             complement(28336..28737)
                     /locus_tag="P5516_02515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91343.1"
                     /translation="MPYVLKDKEGHLLTYGETSFPYNAEKGETLAFVEQSMAEYSRRL
                     VLSHNGSACFTVEAHQGDPAVVIDVSTTLDLPSVELEVNGQLQTIPLVGGKGKLVLST
                     AKPSVFIIKPADRKTFCTAGSSVIVLEVLTR"
     gene            complement(28751..29512)
                     /locus_tag="P5516_02520"
     CDS             complement(28751..29512)
                     /locus_tag="P5516_02520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91344.1"
                     /translation="MPYPNSSDVASGQPTAAAHYNNLRKDAVYLGNVSADSVNLATFL
                     QSFVLNMNIEYLAVARLRVPFDSQKPASVMISGCMLVQTANIDLPAAQFSGGAAVWYI
                     FANRTPGVTSFTLTVNTSPVPGADQKLIGECYWNGTAIAPLSIRSYSNGGDTRLPAYD
                     SGWFACSYNNTYAKAHGLGQFPRECIAFYSATNDNSTYRCGPISASNGTNVIDIVYWD
                     ATNVYIKNFNHASAGAIACSAGNAGSGYYRIFAWV"
     gene            complement(29537..30046)
                     /locus_tag="P5516_02525"
     CDS             complement(29537..30046)
                     /locus_tag="P5516_02525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91345.1"
                     /translation="MSTYTLGQNCHVILSHAEIDSGAPYGFVSPKDGTVRECGAQFIR
                     QVESESTTYTDIVTGTRLWINFDIICADDLLEPNGAEHTPTRLQDYAKLLDFLSKPSG
                     IQCTTPVGTFTNLGALGFSADERHLPKHSIIRVQLNNVGFYFPPADPEKIAASVWDGP
                     LTWETSYWV"
     gene            complement(30043..30693)
                     /locus_tag="P5516_02530"
     CDS             complement(30043..30693)
                     /locus_tag="P5516_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91346.1"
                     /translation="MTKFDDIKTRLRALLEDNSEQRFSDDLLAAATRQTLEELEQRLP
                     RLVSSPFTVTSSGRQQPILSISDCRYILSVSAHGEGETTRELQPETRFTYLLLNGTPT
                     LHFLGQYIPAAGEGLTIHYAAGYTIEGLDGSPSTSLPPALDGVLVDGAAAQVCLLRAG
                     SLLGRYGSDPRESARLMSISHLWRATFERALNGLKVMQEFGFPLGYPLDRWDKAGR"
     gene            complement(30671..31036)
                     /locus_tag="P5516_02535"
     CDS             complement(30671..31036)
                     /locus_tag="P5516_02535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91347.1"
                     /translation="MPADSPELKLAQHLAAPYHRPGQGSRPLSWRSLPDGGMVIISAD
                     GRKLWFTLEEANSAREALGMKPAHPPGLPPVKPTKSRASAISSRPPAGTREGKSEMIV
                     LPPDLKHLEEKIHDKVRRH"
     gene            complement(31017..31220)
                     /locus_tag="P5516_02540"
     CDS             complement(31017..31220)
                     /locus_tag="P5516_02540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91348.1"
                     /translation="MTFLEQLKMMLRSRKFWALMAAIIATLAAYLTQQIDVWQALQAL
                     VAALAVYSTGVAIEDSGNASRQP"
     gene            complement(31312..33336)
                     /locus_tag="P5516_02545"
     CDS             complement(31312..33336)
                     /locus_tag="P5516_02545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91349.1"
                     /translation="MKCWHGEGRQRQQRVSIGQLLKRSPSKLTPPSGKKKEEEMTDAR
                     QSVAEQQRAHFATELRAVAEDSLEIIAISAGNGNGWQFTADVLRDSLPLWEGAECFID
                     HDWKERSLRDLAGVCRDPRWCDTRSGVLLNLRPVGPGAALLKETAAAMYAGEQPSPRV
                     GFSADLLFSAAGRQVIRIEKVLSVDLVMHPARGGEFLPVNDQPGLNREQQLFQTKEKH
                     MEESNHNPATGVDAPGASSHQETQRQLCASLLEISLSAARLPAAAENSLRSRFSGSIF
                     EAAALQQAISDTRALISDLTAGSVIQGAGQVSVIANPEDQVNAALHDLLGAARPRELE
                     GLQPARLSGIRELYTLMTGDMGFTGGYHREHAQFAATSNLPGLLKNAMNKLIFDQWQE
                     LGRSGYRWWEPLVQVEHFNNLQEITGVLVGEVTVLPSVSEGAAYTELAVKDSAETGSW
                     GKYGGYVGLTLEMFERDETHKLRQYPRKLASAALRRISALVGAIFTSNNGVGPVMSDT
                     YNVFESAHHANLGTSALSSASWEAAGKAIYNQPMAVASGGSAPKLALDARYLLVPRDL
                     RLTGMNILYPSFAHESNIFAENMQKGQMGDVITCPEFSDANDWAALADPRLAPGIILG
                     ERFGVLPEIIIADSGANGALFTNDEIRMKVRHWVSVFVADYRPLYKANVA"
     gene            complement(34615..35262)
                     /locus_tag="P5516_02550"
     CDS             complement(34615..35262)
                     /locus_tag="P5516_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 25 protein"
                     /protein_id="HRT91350.1"
                     /translation="MLFGIDVSHHQDWIDWQKVRSSGVRFAIIKASEGEGYIDPMFEQ
                     NMRGAAAAEIVTGSYHFFLPRYDPLAQARHYLRTLQELAGGQATLPPCVDIETPGLGK
                     SGFNQALKVFTEEVFRLSGRTAMLYVSPGFWKSSLPVPVLSNYQLLRSGVDWAAEHPL
                     WVAHYTSGWPCQVYPWAGWAFWQYTSAGQIAGISTRVDLNLFSGTAADLSALVES"
     gene            complement(35359..36888)
                     /locus_tag="P5516_02555"
     CDS             complement(35359..36888)
                     /locus_tag="P5516_02555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91351.1"
                     /translation="MQNFIPHYKWLLREPERFTQMASGITLRKYQRGVLSAIFDSVLN
                     ERGLSLVVIFPRQSGKNELQAQLETYLLTLFSNFPVEMVKVSPTLKPQALTAMRRLER
                     TVKKNKLTQRIWVKESGSTYRIGEARITFLSAAPESNIVGATASALLEVDEAQDVLCV
                     KYEKDVAPMSASTNATRVFWGTAWTSRTLLAQQLRRAREAEKRDGLRRVFRLTADDVA
                     AEVAAYRHHVEETVERLGRAHPAVRTQYYSEEVDGEGGMFPPDRLARMRSAESAEAEP
                     LPGSHYALLLDVAGEDAVARASDGAPASSNPSRDSNALTIVRVEPATEGVPLPCYIPV
                     LRQQWTGVNHTRLHSEVRALALSWQARVVVVDATGIGAGLSSFLERSLPGRVLPFTFN
                     AASKSKLGWDYLGVVDSGRWREPQLDGERQPEQARWQAQFFAQLAASQFQVGSGPDQR
                     LRWGVPDGTRDTASGEPVHDDWVLSAALCACLENLDWSPTSPSFIIPGSDPLTGMEGK
                     F"
     gene            complement(36946..37284)
                     /locus_tag="P5516_02560"
     CDS             complement(36946..37284)
                     /locus_tag="P5516_02560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91352.1"
                     /translation="MTQKKTATTPTRGVQTTRDLTTPEGLDREIDILRELIHKAAAKQ
                     SDDLSLEEQLELLDTVGKTAPALARLLKARRDLNNLDLDPAALLRQALLELEEEWPEL
                     KKFGQKFKNR"
     gene            complement(37567..38022)
                     /locus_tag="P5516_02565"
     CDS             complement(37567..38022)
                     /locus_tag="P5516_02565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91353.1"
                     /translation="MIHLTLLTLLLPMATMPAPALSALQVVIVLAGALLLTLLGGWLI
                     GWFLQPFQAQLNKKFRPNSLRPVSGLKEGGRYIGYLERLLIFVFILSGQFAGVGFLIT
                     AKSILRFGELKDSENRKQAEYIIIGTFASFLYAILISLLARWLLASFTS"
     gene            complement(38060..38734)
                     /locus_tag="P5516_02570"
     CDS             complement(38060..38734)
                     /locus_tag="P5516_02570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91354.1"
                     /translation="MNTNVSLEHAVLTGDIVNSSNLLPRDRQKLFETFPRLSAMLRER
                     YPQAVRHDISNFRGDSWQLIIARPEKSLEISLYIRTYLRFAVSERKLDSRIAIGIGEV
                     EFIPENNVSAGYGSAYTASGHLLETLSGSQRMALGFAAPEDSLAHNAAVILVELVDTL
                     VTSWRASQCQAVYWALQGLTQTQIAQRWHPAPIRQPSVSNNISRSAWPTIKRSLIFYE
                     DAITRV"
     gene            39080..39616
                     /locus_tag="P5516_02575"
     CDS             39080..39616
                     /locus_tag="P5516_02575"
                     /inference="COORDINATES: protein motif:HMM:NF013231.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HRT91355.1"
                     /translation="MTESQGKPQRMRRLLEEMAAGEDIQGMEIARGIRVLNRLYDLAV
                     SRSAEFGELSGARLGILLRLLMEEECGNTAGVNPTRLSHFQDVKKNTITSLLKGLEES
                     GLIERAPDPADRRAALVRISPAGRELVRSTAPARFAFLNRAAAGLDAGERRQLLELLN
                     KLRTSLQPHACCRPSVED"
     gene            39644..41887
                     /locus_tag="P5516_02580"
     CDS             39644..41887
                     /locus_tag="P5516_02580"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91356.1"
                     /translation="MLRIFRYLKPYTLSVLVVILLLFGQAMAELSLPNYMSKIVNIGI
                     QQGGIENAVPEVLRSSQLERMLLFLTPDEQAAVRAAYTPLEPSSPAYDRLIRRFPAAV
                     SGETLARVDLTPEQIETLNPLSGRALLAVSMLEQAASDPAKAAALSQQSGMDLSRLPA
                     GADVFALLHQLPPERLQQMVSIVRGRFDALDSSMITQAAAAAVKTEYIALGADAQKLQ
                     TAYILRIGGKMLLLTLMVALAVIIISLLSARTGAGFARDLRSDIFSKVASFSKAEFES
                     FSTASLITRSTNDVTQMQMVTTMGMRMLFFAPMMAVGAIIRAVEKSPSMTWIIALGVG
                     TLLVMVITIFSVSLPRFQRIQTLIDRLNLVTRENLSGIMVIRAFNSQEFELKRFDKAN
                     IDLTENSLFVGRVMVTLFPLMTLIMSGLSLLIIWVGSHQVAASALQVGDMMAFLQYAM
                     QVVFSFLMISMMFIFLPRAAVSGRRIDEVLRVEPAIKDPPQPKPFDSNSRGLVEFRNV
                     SFRYPNAEENVLCNISFTARPGETTAILGGTGSGKSTLVNLIPRFFDVSEGEIYVDGV
                     DVREVAQADLRQRIGYIPQHGVLFTGTVASNLRYGNENATAEELAQAAQTAQAADFIA
                     EMEGGMDAPIAQGGTNVSGGQKQRLAIARALLKHPPIYIFDDALSALDYKTDARLRRA
                     LANETNRSTMLIVTQRVSTIKHAEQIVVLDDGEIVGKGTHDQLMQSCETYREMVASQL
                     SGEELLR"
     gene            41884..43758
                     /locus_tag="P5516_02585"
     CDS             41884..43758
                     /locus_tag="P5516_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015212237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91357.1"
                     /translation="MSQANTPSRQTMPLRTHGPGGGPPGMLRGGEKARDFKGTMKKLV
                     RYLGPYKFHFLVVLLLAVASTVFNIVGPKILGRATTKVFEGVVSTIAGTGSGIDFVGI
                     GNILLLLAGLYIAAAGFGFMQGWIMTAVSMKLTYNFRREISEKINRLPLRYFDSTSQG
                     EVLSRVTNDVDTISQTMNQSLTQIITSVITLAGVLVMMISISWMMTLVVLVILPTSAV
                     FIRVLVKYSQKYYKQQQDYLGHVNGHVEEMYGGHIVVKAFNGEKRSLEEFNRLNSTLY
                     HSAWKSQFASGLMMPIMNFVGNLGYVGVAVLGGVLAFRGVITVGDIQAFIQYVRNFTQ
                     PLTQIANISTVLQQTAAASERVFEFLSEAEEIPDTDTPVQLPAVRGHVEFRDVRFGYV
                     PEKTIIHNFSAEIMPGQKIAIVGPTGAGKTTMVKLLMRYYDIQGGAILVDGHDIREFS
                     REGLRDLFGMVLQDAWLYNASIMENIRYGQLDATDEEVYQAARTANVDHFIRTLPEGY
                     NMVLDEETTNISQGQKQLLTIARAVLSRPSILILDEATSSVDTRTEVLIQKAMDNLMR
                     GRTSFIIAHRLSTIRNADRILVMNNGDIVEQGTHQELLARNGFYASLYNSQFEEAEIY
                     "
     gene            43946..45226
                     /locus_tag="P5516_02590"
     CDS             43946..45226
                     /locus_tag="P5516_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRT91358.1"
                     /translation="MEINHICVIGLGYIGLPTASMFAQNGVRVTGVDANPRVVESLQQ
                     GKVHIHEPGLTEVVTTVLRDGTLAIASAPVEADAFIIAVPTPFYDDKKADLSHVRAAA
                     ESLVPVLRRGNLVVLESTSPPLTTQNVLRPILEQSGLNAGEDFLLAYSPERVLPGQIM
                     KELVENARVIGGINPASAQAGKDLYLKFVRGEVVLTDAATSEMVKLMENTFRDVNIAV
                     ANEFSRLAERLGVDVWEAIRIANRHPRVRILNPGPGVGGHCISVDPWFLVESAPDITH
                     LIRAAREVNDTQPEFVLQSLKTILPSPQGKRVAVLGLAYKPDVDDLRETPSLEVVREL
                     VLAGATVTAWEPYKPDFKAPNMKMAASLEEAIQDADVLLLLVGHSQFKALDPEVVAQK
                     TSARLVLDAVNAWPSQPWQDAGFTLKKLGNGKPL"
     gene            45226..46368
                     /gene="wecB"
                     /locus_tag="P5516_02595"
     CDS             45226..46368
                     /gene="wecB"
                     /locus_tag="P5516_02595"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015703970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HRT91359.1"
                     /translation="MMTPLRVLSIFGTRPEAVKMAPVVQALKSQRGVESRVCVTAQHR
                     EMLDQVLALFHIQPDHDLDLMRPDQSLVELTSGILTRLDPVLEKERPDWVLVQGDTTT
                     VMAAALAAFYRGIRVGHVEAGLRTGDKRQPFPEEINRHLTTVLADLHFAPTERSRLNL
                     LHEGVPEAHIRVTGNTVIDALQQIIRTDPPAQVREWLDEWGVRPGGRRLALVTAHRRE
                     NFGSPIEHICEALKTLSHRYGNELHIVYPVHLNPNIQEPVYRLLAGIPKVSLIKPVDY
                     ISLIHLIKNAYIVLTDSGGIQEEAAGLGKPTLVLREVTERPEGVEAGVLRLVGSNKDR
                     IVREAARLLDNPDAYTEMARTTSLFGDGKAAGRIVEALLNQPKRPA"
     gene            46387..47412
                     /locus_tag="P5516_02600"
     CDS             46387..47412
                     /locus_tag="P5516_02600"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT91360.1"
                     /translation="MNLFQPCKICILPRKLGLGGPASFQIGLVDAMQRRGITVCHDPL
                     ETGVAAILVFGAAQNTGALTLAQRAGVRVVQRLNGMNWIHRRRFTGVRHFVKAEYGNL
                     KLRRLRKSADRVIYQSAFSRSWWERENSVLDKPQFVIHNGVDLGYFSPHPANLPHDRY
                     RLLMVEGHHGGGYDQGLLTAARLLEGLRCVADRDCELVVVGDVPTNLKQQITSITAPI
                     SWLGVVERAKIPEIDRSAHLLFSGDINASCPNSLIEAMACGLPVVAYATGALPELVSE
                     EAGRLAAYGGDVWKLDPPDISALTDAAWQALQGRDELSRGARLRVEANFDIEKIAEKY
                     IEVLLGD"
     gene            complement(47426..47740)
                     /locus_tag="P5516_02605"
     CDS             complement(47426..47740)
                     /locus_tag="P5516_02605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91361.1"
                     /translation="MLRKQWQKLFKEPGVAGRLLLLLFVLALLAGAMLLSGPKPGDEF
                     DPLATPTPVTLTLNATVAATERPLSSEYGLTDGVILAVITILLVVGIGTLLYTRNSKR
                     VR"
     gene            complement(47754..49085)
                     /locus_tag="P5516_02610"
     CDS             complement(47754..49085)
                     /locus_tag="P5516_02610"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRT91362.1"
                     /translation="MKRLLARSATLLFALVFFITATGCQQLLNEIPFPRRTALATQPE
                     ASPQATLTHDMQKTELPAVVEDKLDKIIFWLPPAFDPENGSSAGRRIQERLQAFRYDH
                     PEVTIEVRVKALSGPGSLLDSLSAATLAAPAVVPGLLILPHADLAIAVSQGLIVPVEA
                     EQNQANQADHFAFAEDLVRVNGLDYGLPFAADVFCLAYKNQQVAYPPVYWQEVIQLGK
                     ILAFPAGEAQGLTTQLLYLEKGGTFTQNETQITVDEVPLQQTLMFLAEGANADVFPYW
                     LTNSNRFEDSWQAVLDAQATYAVVRASQYLSARPENIAITSVPSFSEKRLSLADGWVI
                     AFPASSPEHFELYRQLAAYLVEPRFQAEWTESAGLLPVSRTALSLWKNNEVSGILLEL
                     AESARVVPPAHIISRVGPLFAQATSEMIRQQTTYIESANKIIKSLAEVPLE"
     gene            complement(49082..50083)
                     /locus_tag="P5516_02615"
     CDS             complement(49082..50083)
                     /locus_tag="P5516_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRT91363.1"
                     /translation="MKELIKQLTQIPGPSGYESAIRETIREIIKPYVRETRVDAMGNL
                     IAQVGTKAPGGMRVMVAAHMDEIGLIVSHIEKKGLVRFSNLGGVLPRYLAGGRVVFLN
                     GTRGVINSDRHDEVSKIQPISQFFIDIGAISEKDCPVKIGDLAVFDREFLDLGKRVSS
                     KALDDRCGCALMIETIQRVKQSPHELLFVFCTQEEVGSRGATTAAYQLDADLGIALDV
                     TPVGDILGVKMQVNLGEGPAIKVRDVGHIADPAVVNWMVRTARELKVPHQLEVLDVGS
                     TDARAMQISRGGMPSGALSIPCRYVHSPSEMIDMDDYAHALTLLTGLLSREIKLEKK"
     gene            complement(50108..51181)
                     /locus_tag="P5516_02620"
     CDS             complement(50108..51181)
                     /locus_tag="P5516_02620"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRT91364.1"
                     /translation="MNENRVKISRPQLKLLEKLSNAVGVSGNEDEVRQIVIEEIRPLV
                     DEYRVDRMGNLLAIKHTKTADAARVMFAAHMDEVGFMLVQADTEGCYAFEAVGGIDER
                     QLLAKPVLVGKDHLPGVIGSKPVHLTSPAERAKVTPLGQLRIDLSPACAGKASVGDYA
                     TFATRFWRNGDSLFGKALDDRLGVATLIELARLNPGTAEVLFAFTVQEELGLRGARVA
                     AYDFEPDMAFVVDSTPAFDLPLDESEDENVLYNCRLGAGPAIYLADAGTISDPRLIQF
                     LTRIAEDCGIPYQFRQPGGGGTDAGAIHITRGGVPSVSVSVPGRYAHSPILHARLSDW
                     KNTLALLYQALEKADRSILNADR"
     gene            complement(51178..>51596)
                     /locus_tag="P5516_02625"
     CDS             complement(51178..>51596)
                     /locus_tag="P5516_02625"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91365.1"
                     /translation="TSTFELKPDVGIAVDVTFAQEPGLSTYETFPLGKGVTIGAGGNI
                     HPALHRELKDRAEEIDLPYSIETMPKGSGTDAMAMQISSAGVPTLVIGIPLRYMHTPY
                     EMVALKDIHRAGRLLARFITTLTSGSLESLFKGGAA"
BASE COUNT        11187 a        14157 c        14693 g        11459 t
ORIGIN      
        1 tgaccacacc ctgaaaacct gaaaacgggt tttctggatg agtaaattgc agtgatgaat
       61 ataggggata atcccgtata atcaaaacag agaaatctga ttcaaaagga gcttgaaatg
      121 atcacccgga ttaaacaagg aatgctcatt tttctggtcg cctgtttgct gatttcttgc
      181 accaacactg ccaaaatcag cgcgagtgac aaggtcaaca cctccgtggc gcaaacagtg
      241 gctgccgcgc agcccacagg tggactgccc ttgcctgacg gagcggtaca accaaccaat
      301 cccgcccctc ccccggtcga gcagcccaca acaacaccgc cgccgttacc cacgctggcg
      361 ccatcaatcc ctacactgcc ccccagtccc actactgata cctgtaacca ggccaagttc
      421 gtacaggatg tgacgatccc cgatggcacc aagattactc caggtttggc ctttaccaag
      481 acctggcgta ttcagaacac cggcacctgc tcctggaaca cagcctacgc cgtggtgttc
      541 gatagcgggc acagtagcgg cgcgccaacc gtcacgcctc tcccagccaa tgtgccgcct
      601 ggtgggacga tcgatgtttc ggtggtcatc accgcgccca cgacgaacgg ggattacacc
      661 tggaagttca tgctgcgcag tgacaccggc cgcatcttcg gttttggcgc tggttacgcc
      721 tacccgatga ccgcggtaat caaggttgaa ccgctaatcc tgctgacggc tttaccgcat
      781 tttacattga tacctattgt ttttccgaag tatgatttca cggcgaatta ctgcagcgca
      841 acctggagta atggacatca ggatcttcct tgcccgggca ctaagaacga ctcaacggga
      901 ttcgttgtcc aacaggataa accaagatta caggatggca atacgcgtga tacaaagtca
      961 cttctcactc acccagagtg ggtggatggt ggggcgattg ttgggatttt cccggctgtg
     1021 tcgatcgaaa gtgggaatcg gtttaaaacc actataggtt gcggttacag cggtacaagt
     1081 tgtgatgttc ttttcagggt gaattataaa aagggttctg atccgtttgt tgaattggga
     1141 acctggagca tgaagtattc caacgcaccc ttagacattg atattgatct atcctctctc
     1201 gcgggcagcg atgtgaaatt ccagtttatt gtgtttgcta atgggtcttc ctcacaggat
     1261 tgggcacatt gggtgttccc aaggatcgcc aagtaaccgg aaaaccggga ggtttttagg
     1321 aaatcatctg atgtgaggta gccggtttaa tctgatagaa ttcttggcat gaaacgcaaa
     1381 cactggccca tgctgatgat cgctgttccg attattggcc ttgtggtcgg ttacctgttg
     1441 atgacgcgca atgtgccggc gaccattgat ggcacgccgc tgaccatccg cacacacgcg
     1501 ctcacagttg gcggcgcgct gcgctcagcg ggtttcgccg tggatgaggg ggatgcggtg
     1561 ttccctgctg ctggcacctg gctttcaaag gcggatgaga tccgccttga ccacgccagg
     1621 ttggtgcggg tttatgacac aacccaaaaa caacccattg agctgtctac ttccgcgctg
     1681 actgccgctg aggtcttcaa acaggccggg ctcacgctcg gcagccttga tagcctcacg
     1741 ctcaatgggt tgccggttgg gccaaacgaa aaactcccct tcgggcgcga aatgacactg
     1801 aactatcgcc cggcggtaca ggtcaggctg aatgtggatg gcagagacac gaccatcact
     1861 tccagcgcgg gcactctcgg agaagcgctg tggcaggcgg gattcagact gcgtgaagga
     1921 gaccgggtca ctccagggat ggatacggcg cttaccgaga acctcgcagt tgaaattcac
     1981 accgcccgcc cgctttccat ttctgtggat gggacgatac tggaaggata cagcgccgcg
     2041 cccacggttg ccgcagccct ggcagaaaac ggggtgaccc tgcaggatat cgataccagc
     2101 cagccaggcg ctggtgatcc gctcccagag gatggcgtca tcaatgtcac ccgggtgaca
     2161 gaagaaattc acgccgaaca gcgcgtgatc ccctttgaaa ttgagtcgtt ggctgatgac
     2221 acaatgttca ttaatgaaac caacatcaca caggccgggc agaacgggtt ggcggctacg
     2281 cttgtgaaag tgcgctatga agacggtctg gaggtttcgc ggcagaccca aaacgaggtg
     2341 atccttatcc caccggtagc ccaggtggag taccgtggaa cgaagatgct tgaaaattac
     2401 atcgacacac cagaaggagc gcttcagtat tatttggtca ttgatgctgt tgccacttct
     2461 ttttctccgt gccggcaagg cattgaagga tattgctcaa acggcaccgc ttcggggcgc
     2521 accattcaaa aaggcgttgt ggcgttcaaa cgcaactggt ttaacctgtt tcgcgatacc
     2581 agggtctacg tgcctggtta tggcattggg gttgtgggag acacggggtc ttacccgtac
     2641 agcgatagct ggatcgacct gggttacagt gacgcggatt atgttccctg gggagcggtg
     2701 aaggttaaaa tctactttct ctctccaccc ccgtcagggt tcaccggggt gctgccatag
     2761 aacccgcgcc gctggatatt cactcactgc tgaaacgcca tcatcttgac ccgcgtaaag
     2821 gtttgggcca gaattttctg gtcgaccatc acgcgctgcg caggatagta aaaagcgctg
     2881 aactgactgg tggagaagaa gtgctggaaa tcggtgccgg gttgggcagc ctcacccgcc
     2941 tgctggccgc cgcggccggg gtggtgcggg cggtagaaat cgaccacagg ttgatgcctg
     3001 ttttacgcga ggtcctttcc agttacgcaa atgtcgagct ggtgcagggg gatattctgg
     3061 cgctcgaccc cgccaggctg atgaatgaac ccgggtatat cgccgtcgcg aacatcccct
     3121 attacatcac ttccgccatt gtgcgccact tgctggaatg cccaaagcgc ccggcgcggc
     3181 tggtgctcac catgcaaaaa gaggtggctg agcgcatttg cgcagaaccc ggagacctct
     3241 cgctgctggc gttaagcgtg caggtttttg gtcaaccaag catcagcgct gtcatcccgg
     3301 cgggggcttt ctacccggtt cccggggttg actctgccac gctgcgggtg gacctgcacc
     3361 cgcagccata ctttacagat gaacagcgga agatgttttt ctctcttatc cacatggggt
     3421 ttgctcaaaa gcgcaaaacg ctgcggaata acctggcggc tggcttacat cttagcggcg
     3481 aagaagccgc gcgatacctg gtagacgcgg atattgaccc gcagcggcgc gcgcaaacgc
     3541 tcagcctggt ggaatggaag acgctggtga acgagttgaa agacctgctg ggagcgaaaa
     3601 gagagcagga aaccccgcct gcttaacccc gcggcagggg ctggatatca cggtaacgag
     3661 agatcaagct ccacacccgc aaggcggctt ccacctgaat tgaaaaattc atcttggatt
     3721 ttccgcgagt acgttcagcg aaataaatag gcacttcttt gattttaaac ccaagtctgt
     3781 gcgccaggta ggcggtttcg accatgaaaa cgtaaccatt ggaccgcacc ctatcccatg
     3841 gcatcgccgc cagtgtctct cgccgccaca acctgaaccc tccggtgagg tctttggtgg
     3901 gtatacgcag aatggtgcgc gcgtaagtat taccgaagtt ggagagccat tttcgccagg
     3961 ccggccagtg gcggtccagg ctgccgcccg gggtgtacct tgagccgaaa accacatcgc
     4021 taccctcgat ggatttcata aactccagca gtttttctgg ctggtgagaa aaatcagcgt
     4081 ccaagtgggc gatgtacgcg gtgtttgttt gcaaaacctg cttgaacccg gagatataag
     4141 cggtgcccag gccaagtttg cctttgcggt gcatcactga gatttgccct ttgtatctct
     4201 ctgccagttc atctgccagc gcgccggtac catcagggct gttgtcatcg atcaccagga
     4261 tggaaagatc ctccagcggc agggcgcgca ggcgtgcgac gatgagcggt aaattttcaa
     4321 cctcattata agtcgggatg atgacgcaga tgctcaagga ttctcctgaa ttaacgccaa
     4381 ggtgggcaaa cagcgtaatt ctaccagttg aagggaagat tggggcaggg aaaatcaggc
     4441 gtgaatgctt cttttaccgg gcttacgtgt ttaggccgtt tccattaagc aggcgcgcca
     4501 ggtcttcggc ggttttgcgc agctccaaaa aaaggatgcc cagcttggct tccggctgca
     4561 ccagcgtggt aaaaacggct tctttcccca ctgctagtaa aaccacgtaa ccgttgcttc
     4621 ccctggtgta aagctgctca agcgtgccca gccccgtctc tttattgatt tgctctccga
     4681 gagaaagcat ggcagcagac atggcagaga tgcggtcttc gctcattcct tctggcagag
     4741 cggatgccat gatcaagcca tccaggctga tcacagccgc ggcctgcact tctggcaaac
     4801 tgtgctgcag aacgcgtata cgatcgatca gggcttgccg gtcaattaac tccaaaagcg
     4861 tccctctttt tcgttgctga tatttcccgc tggttcctat tttgacacag ccactggcgt
     4921 atgtcaagaa aatcgttcaa gcccatcaca aaggctttcg attttgtttg agcacgatca
     4981 gttgtgcaaa aaggatcgcg cccaggatga ccacgcatcc aatcacctga accaggttta
     5041 gttgttcgtt caagaaaagc acacccgtga tggcggcgaa caccgcttcc aggctcaata
     5101 agagcgcggc atccgctgaa ggggctttct tttgcccgat ggcctgcaag gtgaaaccca
     5161 gcacgaccga gaacaaagat gagtatagca atggcagcca gctggcgcgc agtgcgggca
     5221 tgctcaccgg ttcaatgaac atgctcatta ccaggtggat caaaccgcaa accaaaaact
     5281 gacccacaga aaagacaagc acgtcgattt ttttcactgc cagccccacc acgatcacgt
     5341 gcagcgccca taccaccgag ccagccagca ggatgaggct gcccgtggag gggatcagca
     5401 cagtcccgcc agtgcttaac aggtaggtac cagcgagcgc taccagcgca gcaacccagg
     5461 tgaccactgg cgtgcgttga cgccagaaca acgccagcag gacaggaacg agcaccacat
     5521 aggtcccggt gatgaagccg gcagtgctgg cagaagtggt gccaatgcca atctgttgca
     5581 gcgagctggc agaaaacagc acccctccag caagcaatac ccaaccccaa ccacctttga
     5641 aggcgcgaaa acgccgccaa acaaataaca gcagcaccaa cccgccgagc aagaatcgaa
     5701 taccattaaa cgcgttaaag cccaggtgaa atgaagccat acgctgggcg acaaaaccag
     5761 caccccagat gatggcggcc agcagtagat aggtatctga taccagcctt tcgcgcaaat
     5821 tgattttttc agtgtcttgg ttcatcgatt tgttgattgt acccaaaaaa tgaaaaagcc
     5881 acccgggatt ttgcgcccgg gtggcagggc aggttcttta gcggttgtgc ttgaattgcc
     5941 ggctggcgcg ctgcatgcgc cattgacggg cgaccctggc gagatcggtc gataatttgg
     6001 ctttcaccca ttcgctggca aacttttcct gcgggagcgc caacagcttt gtgtgcattt
     6061 cgtccagaag ttcctctgta acgccatttt cttccacgtt ttccatccat aaagcttttt
     6121 cagtctcgtc gaacggaatt tgttgaagta gatgaatgat ctgatgtttc tcagcctgtg
     6181 ttttttcagc catatttcct ccttcagcgg gcatcttcac cgggctattc tattgtacac
     6241 gattcaaccc tctcttcagc gctgcaactt tctaaccggt gaagcgtaaa caacatgaag
     6301 gatatcacag gagatgaata tggataacaa cacaaaaaag ctttaccgct cgagggttga
     6361 ccgcaagatt ggcggggtct gcggcgggct gggcgaatat tttggtattg accccacgct
     6421 tgtgcggctg ttgtttgtgc tgggtttact ctttgtgggc ggcacgctgc tggcgtacct
     6481 catcctgttg atcgtgatcc ctgaagagcc tttataaact gttagcccag ccagaagaaa
     6541 agaaccaggc tgttaccgat ccaggcttcg ttcgctggct cagtctgcgg cgttattcgt
     6601 ggcgcagggc ttctaccggc tccaggttgg ccgcgcggtt tgccggataa agaccgaaga
     6661 ataatcccac cgcggtggag aaaagcgtcg ccagcaggat ggcatccagg ctgacctggg
     6721 gggtgaaagg ggtgttgttg gccaccgcga tacgcccgac cacataagaa atcagccacc
     6781 cgagcccgat gccgataatg ccgccaaaaa ggctcaacaa cgaggattca gtgaggaact
     6841 ggatcaggat atccttttta cgcgcgccga gggctttgcg cagcccaatt tcacgggtgc
     6901 gttctgttac cgaaaccagc attatattca taatgccgat gccgccgact agcagcgaga
     6961 tgccggcaat accacccaag aagatagtga gcacaccggt gatggtcttg gccaggtcta
     7021 caaaatcatc ttgcgaaaaa atggtgaagt catcctgccc aatacgcgta cggtggcggc
     7081 tgcgcaggat ttgagcgatc tcttcagttg cctgcgtggt cagttcgggc gagataacct
     7141 gcacgaggat catatccagg cggttatcag ggtggcggat gaggcgcgcc tgcgcggtgc
     7201 tgagagggat gatgagcagg tcatcttgtg agccgaaaga tgagccgcct ttcgtctcta
     7261 ataaacctat cacgcggaag ggctgccctt cgatgcgcag ggtttcgccg accaaccctt
     7321 cacgccgccc gaagagtttt tcagcggtgt ctggaccgat gagcgccaca gaagacctgc
     7381 ccagcagctg ttcttcagaa ataaaaacgc cttctgttaa cccgtaattt cttatctgtt
     7441 gataggcagg ggtcaccccg taaacggtcg ttcgggtgga ttcaccagcc gtgctgacca
     7501 cgagtgaccc atttaccaca ggcgccacac ccgctacgga tggcgcctgg aactggtcaa
     7561 ggaaggcttc tgcgtcttca ctggtgaggg gctcgatgcg ggtgagctct acagtaggat
     7621 taccgcgaat cacgaatagc aggttgctgc cgatgccgct gatggaaccg gtgatggttt
     7681 cctgcgcgcc agcgccaact gccatcatag cgatgaccgc gccgacgcca atgacaattc
     7741 ccagtatggt cagaaatgag cgcagcttgt tggaatacag gctttcaagc gcctccacga
     7801 tcgcctgcca gaggttcatg gcgtatctcc ctctaccagg ccatctttta agcggatgac
     7861 acgctgagtg ttggaagcca cgccctgatc gtgcgtgacg atgatcaacg ttgtgcctga
     7921 attctggttc aatttcaaca gcaattccat gatctcctga ccggaacgag aatcaaggtt
     7981 gccggtaggc tcgtcagcca tcaagatggc ggggttgttc accagcgccc tggcgatggc
     8041 cacgcgttgc tgctgcccgc cggagagttc gggcgggcgg tggtgcatac ggtcagccaa
     8101 gccaaccgat tcaagcgcgg cgatcgcccg ctcgcgcttc tcttttgtca cccccgcgta
     8161 acgcatgggc agctctacat tttcgatcgc gctttgccgg ggcagcaggt taaagctctg
     8221 aaagacaaac cctactttgc ggttgcggat gtttgccaga tggtcgtcat ccagttgggc
     8281 aacctcttct ccatccagcg tgtaagtgcc gctgctgggt cggtcaaggc agcccagcag
     8341 gttcatcaac gtggacttgc ctgagcccga gggacccata atggcgacga tctcgccgcg
     8401 gtcgatggtg atactgaccc ccgcgagggc gtgcacggac acttcgccaa gctggtaaat
     8461 tttggtcagt tcatgcgttt gaatgacggg ttccataaag gtgccttaga aaggcggtct
     8521 tccgctgaaa tgcacatcca taaaggagga aggcgggttg agaatgacct tatccccttc
     8581 tttaaggtcc cctgagagga tttcgctcat cgaatctgaa gaggcgccaa tggtgatgct
     8641 cacttcagtg gggatgttgt tcttcaggat gtagaccact ggatggttat ttaccaggcg
     8701 gattgcgcgg ttggggacgg tcaacacatc ttccagctgg ctgacgatga tgttcacccc
     8761 agcggtcatc ccgggcagca cctgctcatc agggttgagt atctcgaccg tgaccttaaa
     8821 attgaccagc ccggattcaa tcgtgcctac ccgggccaca cggttcacct tgccctggta
     8881 agcctggttg ttaatggcgt cgaaggtcag ctcaacaggc tgaccgacca cgacacggtt
     8941 gatgtccacc tcgggaacct gcacgtccac cagcagatga gagaggtcat ccagtctgaa
     9001 agccacccct ccgctggtaa ccaggtcacc gggcatgata ttgacctcgg tgaccgtgcc
     9061 gccaaagggg gcggaaatat gcgccagcgc catggtggcc ttaatcgccg cgatacgagc
     9121 cttggccgcg gcgatatcgt tggggtccgc cccgtccttc aggcgttccc attcgcgttg
     9181 ggcgtcttca tattccgcca gcgctaccgc aaccttgccc tctgatatgg cgatttcttt
     9241 ttcattgggg ttctgcacca ggtaatcgag gtctttgatg gctttatcca ggctttcacg
     9301 cgcgttggcc agcgtgctga gcgcgttggc cttcagcggg tcgtcatcat cgcgttcatc
     9361 cactttgtcg tagttttcct cagcggtgtt cacccggtcg cgagccaggg tcactgcagc
     9421 ctgggctgcg tcgatcttat tctggttggt ggtacgggca gtatctccat gccagcttga
     9481 ccatacggcg cgttcgtaag cgtctttggc gttggccagg tttaattgtg cccgcgcctg
     9541 gctcaggttg gaatccagca ggttatcgag tgcgcgttgg gcttccacca ggtctgcctg
     9601 cgccaaaatg atattttgcg gtagggagga ttcgataagt tctgccagga tggtttcgcc
     9661 ggcaactttt tcacctgtct tcacgtgaat gtccccaacc ctgccgctgg tttgccagac
     9721 gagcaccgcg ctctggttgg cgcgaaccgt gccggtggct ccgataatgg caaccagttc
     9781 acccctggtc acttcaactg tctggagttc ggcggtggca gctttgcggg cgctgagggt
     9841 attgagcatc aggtaggtga tcacactggc agccgccaga ccgaggagga tgaacaggat
     9901 ggttcttttt gtcagtcttt ttttcatagg agcttaacgc cttctcttga tgatggtgcg
     9961 ctcagcgtga acaggtataa tttgcgcatg acctggcgta aatataccgg gattgcggtc
    10021 ccgctattga taatgatggt gttgttatgc ggctcggttg tttctttagc gaatccgctg
    10081 gaccaggcgc ggctgttcac tgccgatatc gaatatgatt atatcgcctg gttggcaaat
    10141 gcgtcagggc aaaaggcctc tcaagcgctg ttaaaccccg ttcaaagact gccgcaggaa
    10201 cagcaaaccc ggttggtgaa gcgctacttt acgcttgtgc aggcgttgga agttgtgcag
    10261 gcgagggtgg aactggtata cgcagaccct gccatcagtg atgccgcggc agccgccgct
    10321 gaactgatca ggcagcagca cgcgctgcaa cagtcgctgg atggtctatc cccattggtg
    10381 gaagccatct tgcaattcca agttgccagc gtgatggcag acgaggggat tggttttctg
    10441 gcgcagccgc tgcccccggt gctttttcat tcttcgccgc tgccaaaagc cctgatcgta
    10501 tctccgcgcg attcgattcg ccaggaagtc aacatctccc tgctggctga cctgggattg
    10561 gaagacatca ccaggcttga agaaaatgtg tcgtcttata tgaatgcttc ggcgctggtg
    10621 gtggatgtag gcgggattgg cgtatacccc accatggtgc agcgttcttc gaatatcgaa
    10681 tggacgcttg acaccatcgc ccacgagtgg acgcataatt acctgaccct gcatcccctg
    10741 ggttggaact atgacaccac ggctgagctg cgcaccatga acgagaccgc tgcttcgatt
    10801 gttgggaagg agatcagccg cgaagtgatg cggcgctact atgctgatga tgacacgggc
    10861 gaaaacaaaa cgccgaaagc gtggttaaaa agaactgccg tgaacgagtt tgattttcgt
    10921 caggagatgc acaaaacccg cgtaagggtg gatgaattac tggcgcaggg caaggtggaa
    10981 gaagccgaag aatatatgga gcaacgccgc gtggtcttcg tggagcacgg ctatatgatc
    11041 cgcaaactga accaggctta ctttgccttt catggcgctt atgcggatat gcctggcggg
    11101 gcagccggag aagacccggt aggtccggca gtgcgcgcgt tgagagagca ggcgggttca
    11161 ctggcgctct ttttgcgccg catcgcctgg atgaactctt ttactgacct gcagaaagcg
    11221 ctcgcgcact gagcgctacg tgcctcgcag ggaaagactg aaaggcgtga tcattcttcc
    11281 cccagttctg gtaaatcgcg ctcgatctcc tcgggggttt gccctttttc caaccggctg
    11341 accacctcat cgaactcacc cggaaggtca tctgccccta cctcgttttt catctcccgc
    11401 atcatgcgcc ccagcgcttt gggatcatgg tcaatcatat ccaggtcgct gtcgttggcc
    11461 aacgactcca ggcgtcctgc atctccgcgc gcgacgcgca ccttacggat cacacgttgc
    11521 aggtcagtgg aaccgcaact ggggcaggcg gtctgatggt ggtcatattc cgcgtaggtg
    11581 aggaacactt caaaacgctg cttgcaactg gaacaacgaa aatcatagat cggcatagct
    11641 acctcagttg attactgaca ttatccatta taatcacagc atatcagaac tgtgtaaaaa
    11701 ttcccctgag aggttcagca tgtcttctga agaggtgtct tttgacctaa tccacccgca
    11761 gcccttgttc atcgttatat ccggcacttc aggggtcggt aaagacagcg tgatcagagg
    11821 gctgaagcag cgcgacttac cgctgcatgt ggtgatcact gccaccacac gcgcgccacg
    11881 cgcaggagag gtggatggga aggactattt tttcttcacc ccagatgaat ttaaaacccg
    11941 gcaagccgaa ggctggttcg ccgagcacgc cgtggtatat tctgatctta aggggattcc
    12001 gcgccatcaa atagaagaag cgttggcgag cggcaaggat gtggtggcac gcgtggacgt
    12061 gcaaggcgcc agaaccttgc gcaagcagta tccgcaggcc gtgctgattt ttatcgttcc
    12121 tgggtctttt gatgaatggt accaacggct aacgcggcgc ggcactgagt cagcagatga
    12181 cctgcggctg cgcgtggcga aagcccgtga agaagtaaaa cagatcgggt tgttcgatta
    12241 tgtggtggtg aacccggatg gacagatcga aaaggcggtg gatgacgtgg tttccattat
    12301 taaaaccgaa catatggcgg tgaaacaccg ccgtatcctc tgatggaacg cttcgcgggc
    12361 tctcaggaag aacagcagcc tcagccaccg ccggtgggga cgcctgttac agtaaggctg
    12421 ccggttagaa aaccatgggt gacctatggg ctcattggca tcacgattgc ggtgttcctg
    12481 ttacagttgc tctctcaatc cacaagccac gacggcgttg attggccttt cctgctcggc
    12541 gggaagatca atgcctatat cctgcgcggc gagctgtggc ggctggtgac ccccgcgctg
    12601 ctgcacggca gcctgctgca catcgctttt aatatgtacg ccttgttctc gctgggcagt
    12661 tcattggagc gttactacgg ccatggcagg ttcctggtgc tctatatcat cgggtctttt
    12721 tgcggcaatt cgctctcttt cctgctctcg ccaaaccctt cgctgggcgc ttcaacggcg
    12781 gtgttcgggc tggtggctgc cgaaggcgtt tttatttacc gcaacaggag gttgttcggt
    12841 tcgcgggcgc gcgccatgct ttcaaacctg gtgctgatcg tggttgtgaa cctgctcatc
    12901 gggttgcaac cagggattga taactggggg cacctgggcg ggctggctgg aggggcgttg
    12961 tttgcctgga tggctgggcc gctgctgctg gtacaacaaa catccaccgg ttacgaactc
    13021 caagattcca ggttgagcgg cggcttactg tgggggatcc tgctttccgc gggcctgttt
    13081 agcgctatgg tgattagcag gttcattacc ggagactgag aagcgggaac atttgcaaca
    13141 aaaagcgggg agttggactc cccgcttttt tggttgatac gctggtctta tggttcgctg
    13201 acctcaaacc agggaacgtt catggtggtc caagacttgg gtgcctggat tgaaaaaccc
    13261 accttgccct tcttgaggtt tttattggtc acggtttgag aaaagacatc attgatgtaa
    13321 actgagagct ctttgccctt gcattcaagg gtgtatttat tggtgtcttt gccgtagtgg
    13381 aacttttcag cgccgccatc cgataagaag ttccatttca ccaggggttt atcaaaataa
    13441 tacagcctcc atctccctgt gttgctcaca atgagttcat accagccaag ttcctcatca
    13501 taccggcaga ccaatgctgc ttggctggca gggaaaccca ggttttcgac ctcaacagag
    13561 attttcacat cctcataaac ctggggtcga taaatcatat agtagttgag atagtaccgc
    13621 tcgatattga attcaatagt accgtcttca agctcgactt tgattttctt ttcaagttct
    13681 tcgttttctt taataatttt atttactttt acagtgatgt cgtaaatgaa gtggtcgtac
    13741 tggtccaacc cattatcaaa ttcatccagg aagtaccgtt ctccttgcgc gctgtcttct
    13801 gctatgaacg gcgttggttc gtatccaaat ggcggcgttg gtctagttgc cgcatctggt
    13861 ggctccgttg gcggggcggc gggaggttca gttgccacta tcggcggggc catttcctcg
    13921 gatgtagcag tgattataat gacctgagga gattcgggtg tggcagtgat gatgatgacc
    13981 tgagggggtt cggttgtggc tgctcctttg gaattgccac aggcggagac gagcaaggtc
    14041 aaagccaggg caatcatcat caatgagatg aacgcttttt tattcatcgc tttctccttg
    14101 ttgtcttact tatctaattt tggatggtac ttatatagta cacgagattt cttttatttc
    14161 aagccccatc cgcaccatgc attggcatgt caggtcttct gaggtgctgg acaattacgg
    14221 ttgactgata tcgaaatact caaagtccac gttcaccggg aattggttaa aagaggatac
    14281 gcccagccct acgcgccctt ctctcaggtc tttgtgctct acggttttga ggagcacatc
    14341 gtttacatac aacgagagtt gagtaccaat gcactctgcg gtgatcttgt tgatctgcct
    14401 gcccatttta atgtcgttgg aaccgccgtt ccataacttc acgtgacctt taacgagcgt
    14461 gttgtcgtag taattgatac ggaagagccc attgctgctg atattgaact cgtaccagcc
    14521 taattcttca tcgtagcggc acaccagggt gatgtggttg tcgttatatg ccaggttttc
    14581 agcgcttact tccaggcgca catccgtgta agtgtacggg tcataggtga ggtaaggcgc
    14641 aaggtacatc gcgttgaggc gcacctttaa ttttccatct tcggtgtaaa tcttatcttt
    14701 accgaaacgc cctgtccata atatctgttc aaaatcgtca agacctccat cgaattcttc
    14761 acggaagtac ttgggcggtt cttcaccccg gggtgtttcc gcatcgggcg ctgcaagggg
    14821 ttcggtaggc tcaggtgtgg cagaaacctc cgctgctgta ggagttacca cgatcacata
    14881 aggggtttgt gttggttgag gagtgctttc gattgctgca gctggtgcgc aggcgctgcc
    14941 ggctacactc accagggcgg tgatcgcgac gacgaaaatc cattttttta ttttcatatt
    15001 atccttcttg tgttcaagag aagcctttat gaggttgaac gttggctgac tgttctttga
    15061 agaaatgagt catcattatt gaagaaagcc ctaaaatcca ccgtctccgt agaagctggg
    15121 gtcggtcggg attttccctg atggatccca cacgtacaca tagtcgccat cgttcgccca
    15181 gttgaacatc cagtgcgcgt ctgccacgct gagattgacg catccatgcg agttctggta
    15241 gccgaggaac ggctgccagt aagcaccgtg gatcgcgcgg gctttgtcat aatacatgat
    15301 gtaaggtact tcttccaggt agtagtaatc ggagcggtcg acttcgaaag aaccgctcat
    15361 ggggtcggat tccagttttt tgtaaatctg gaacacccca ggctgggtga agaaagggtt
    15421 gactcctgag gcgatgaggg tggcaaaaac catctgacca ttttcatatg cggtcagcgt
    15481 gttttcatac aggttgatct caatccactt gtcaactggc acgcctgcgg ggcgctgata
    15541 gttgggaatg acgcgcgata taacccggtg ttccacccac tcgctatagc cgatcatcac
    15601 ccactcggtc tcgttgacca ctgcagagtc ataaaccata acggggtcca gccgccagta
    15661 gttcacaccg gtttcggggt attcgtaacc aggaccagcg cggctggtcg tttcattgat
    15721 gatccagcca aaggaaaaat ccggactttc tttaagaatg tatccctgga agttggtcgc
    15781 cgcgactttc ttggcgagct ccacgctcac ccattcggta gtggcgatct gatagaaacg
    15841 accacgcgag gtctcagcgc tgttcattaa cgccacgtat ttggtacggc tttttggcag
    15901 gacgttgctg ggcttaccac tttcgacgtc agcgagcgtg gcgtacaggg gaatttcctg
    15961 gtcatcgacc agtttggcgt aagagaaggg tgtggcgccg agttcatacg gcgtccgcga
    16021 ggcgaacagc tgccgtccct gcggggggat tcccctggcg gccagttctt caaggcgcgc
    16081 ggccgggccg gctttcaggc agccgttcat cagttcttcg cttgtctcgg gcaggcacag
    16141 ggcgctatca gcttcacttt taccggggga agtgagcgcg gaaaccccct gtacccgcgc
    16201 aaacaaagca gcgaaaacgg cggtgaggat caacacggca aggctcagtc ttggtttcaa
    16261 tgcacaattc ctcgaatcat catattcaga tatattttat cttaaaaaga tctgttcaac
    16321 ctatcacggt ttttccccca cccatggcaa agcttggcgg tcttactccg gctcatcttc
    16381 ggtgtttcgt gaagtgaact gcttaaggca ggaaaaagtc tgttcaccca gcagatattt
    16441 cagctccggc tcgaaggtat cgagtaacgg gatgggtttg ggcgcgcgtc tcaggcaact
    16501 tccctgctcg ttcaccatat cttctgcggc gtctaacgtg atcctgcctt cttcatccag
    16561 gtcgaggggg tgcagcacac aataaaatgg tttaaaacgc cagggatgca ggttatttgc
    16621 cagcgccgcc acctgcagcg cgcatttgcc atccgcgagg cagaaaacgc aggcggttcc
    16681 ctggtaatgc tcagcccggt tttctaccgc tgtatggatg acccgactgc ttgggctgcg
    16741 cttatctctg tcttccaccg cggcaaacca ctcgccgggt acgcggcagt ccggcggcat
    16801 atgcggcagg atgatggcag cgttgcgcat aatatcatcg acctcgcgca ggtctaccca
    16861 cacgccaaag acgcagcagg tgcctttgca atctgcaagg ctgcagcgct gaatggcttc
    16921 cgattgaagc aaccgcgggt taacgcggct gagcgctttt ttcgtggtag cgtccatgag
    16981 cagattgtac cacctccaaa atgcgcgaaa ttctcatcaa aaaaaggcgc tgttaatgaa
    17041 ataaggtaaa atggatgggg gttttccccc aacagtctat acaaaaacaa ggagtaagaa
    17101 tggctgcctg gaaggtgctg ttgacggatg gattggaaga gaacggaaaa gagatcttgc
    17161 ggtctgcggc agaggtaaca gaccaatcag ggatcagcgc ggaggacctc ttaaaggtcg
    17221 tggggcagta tgacgccctg atcgttcgcg gtcgcaccaa ggtgacggag tcggtgttgg
    17281 ctgccgcgcc ccatttaaaa gtggtgggca gggcaggcgt tggggtggat aacatcgacc
    17341 tggctgccgc gaaagcgcac caggtgaccg tagtgaattc gccgttggcc actaccgtgg
    17401 cagttgccga gctgaccctg agcctgatgt tgagccttgt ccgtgaactt ccacgcgctg
    17461 atgcttcaat gaaagcgggt aaatggttga aaaaagaatt cgaagggcgt gagctatttg
    17521 gcaaaaccct cggtgtgatt ggtttcgggc gcattggctc agccgtggca gcgcgcgcca
    17581 aagccttcga aatgaaaatc ctggcttatg acccgctggt ttcagccgaa gaaatcaaga
    17641 agcgcggtgg tgaaccggtg agcctggatg aattgctcac tgctgctgat atgatcacca
    17701 tgcacatgcc tctcacagct gattcacgca atttactgaa cgctgaagcc cttgccaaaa
    17761 ccaaggcggg cgtttacatt gtgtgcgccg cgcgcggcgg ggttattgat gaagaagcgc
    17821 tgctggctgc tttaaactca ggtcacgttg cgggcgctgc gctggatgtg ttcgcgaacg
    17881 aaccccctgg ggcgaccgag ctggtggcgc acccgcacgt gatcgattca ccccatattg
    17941 gcgcacagac tgttgaagcc caggcgcgcg cggccaacga tatcgcggaa gaaatcctca
    18001 acgcgcttgc cggcaaaccc ctgcgctgga aagtcgcctg aacatgacag gagagatgat
    18061 gcaaaaaaag gtagagcggt ttaagcgcat gatcatggaa gtcaccgacc tgggacacgc
    18121 ggaagccgtg ttggggtggg accagcaggt gtatatgccg cgcggtggcg gagaagaccg
    18181 cggagatatc ctggaaacga tcgccagcct ggcgcatcag aagttcactt gcaacgagat
    18241 gggagaactg ctggcagaac tgaaacccta cgcagaaaca ttggacgcgg attcagatga
    18301 cgcctgcctg atccagcgcg tggcacaccg ttatgataaa gaaaccagag taccgacgga
    18361 atgggtggtg gatttcaccc gcgtgaccac gctggcgcaa atggcgtggg aagaggcgcg
    18421 agcaaagagc gactttacca tcttccaacc ccatctcgaa aaagtggttg acctgcgccg
    18481 cgcatacgct tcttttttcc agccttacga ccacgtttat gacccgctgc tggacgggtt
    18541 tgagcccggg ttgaagacag cggaggtgca acagatcttt gacgcgctgc gaccgcagca
    18601 ggtggcgttg ctgaaagaaa tcgccgccaa acccgagatc gaagatgcct tcatgcacgt
    18661 cccttacgat gaacagggtc agtgggattt tggcgtggag gtaatcaccc gctttggtta
    18721 cgattggaac cgcgggcggc aggataaatc ggttcatcca ttcaccacca gttttgggat
    18781 taacgatgtg cgcatcacca cacggtttga cccgtcgcgc gccgcgtcag cgctcttcag
    18841 caccatgcac gaggctggtc acgccatgta cgaacagggg ttctccgcca gtttgcggcg
    18901 attaccgctg gcagacggcg cttccatggc cgtgcatgaa tcgcagtcgc gcatgtggga
    18961 aaacctggtg gggcgctcga aagccttttg gaagttcttc tatcccaggt tggtgcaaaa
    19021 attcccgcag cagcttaagg gcgtcagcct ggaacagtat taccgtggca tcaacaaagt
    19081 tgagccttcc cttatccgcg tcgaagcgga tgaagccaca tacaacctgc acatcatgct
    19141 gcggcttgaa cttgaaatcg gcctgatgga cggcagcgtg cgggtggcgg atctgccggg
    19201 gttgtggaat aaaaagatgc aagaatacct gggtgttaca ccgcccaatg atcgcctagg
    19261 cgtgctgcag gatgtgcact ggtcaggcgg gatgatcggg tacttcccca cctatgcctt
    19321 gggtaacctg gtatccgcgc aattgtggga aaaaatcatg gaaaacatcc cagatcttga
    19381 agaccagatc agccggggca agtttgatga cctgcttgcc tggttgcgca cccatatcca
    19441 ccggcatggt gccaagtatg agccgcaaca gttgatcaaa aaggtcactg gcacaggcat
    19501 ccgcccagac ccatatatgc gctacctgcg cgcgaaatat gcagaaattt acaacctgta
    19561 accggtaaag aagatggatg cgcgggacgg ccagctgccg tcccgtttgt atttaaagtt
    19621 ttgccaacca gtcggcagta tgaaaccaat aacggcctga atggaagggg ttgaccaggt
    19681 caggcgaatt gatagccaag gaaaataagt ggagaatgac agcagtgctt aaggaagtac
    19741 cgccatcaga gtgatgatgg aaccagccca ccgctaaagg cgcttcagcc gcgcgggctg
    19801 cgccaggcta ctgccgggca acaaacggga tcaaggcgaa cccgggctgc ttacagatta
    19861 ctcgatataa acctcgacat gctcgccgtc ttcatcatct aacacatcga cgatctttcc
    19921 ggtttcgccg gaggccaaag cctcaaccag gcgcgcggcg tcgagaccct ccacttcggg
    19981 agaaaagcgc atacccatgc gcaagccggc attgaccatg cccaaaggca ggcgaatgtt
    20041 cacccgggtg cgcccggtgt cagtatcggt cacacgcacc ctgaaaaatt ttccgccgcc
    20101 cttgccagga aaattcaccg cagcgcgttc gtcaacttca gctttcttgc tggcggcgtc
    20161 cagggctttg agcagctcaa cgcccgcttc agcggtgatc tttccctcct gcaccatttt
    20221 taatactttc agtctttctt cgccagttgc cattacctgc ccccttctgc cgcgttgtgc
    20281 tgagggatga acttctcgcc gcgcaatatg gcccgtgctt cctcccaaga gatctcgccg
    20341 cggtcaagct tttccaggat atcccgccta ccctctggtg atagttgagc gggcccttca
    20401 tctttgcctg gttcgtaacc gagcgcgcgc acgatttcag tgaggcgact gcgcagggtg
    20461 gggtaagaga ggtttaattc gtcttccatg cggttaaggc gcccctcgca cttaacgaag
    20521 gtaagcaaaa attgcatctg ctcaggtgtg aggttgccaa aaggggtgtg ttcagcagcg
    20581 aagtgaccct caatggtggt gtcgcaattg acacaataca ggcgggtgat catcagttca
    20641 gattcacata cggggcattg atccaatgtt ttattgatca tggtattttc cttcgattgg
    20701 tttgatagaa agaaacgcgc actgcggggg ggggttctcg ccagttgttc acgactttgt
    20761 tttctaaaat accaatcaaa ccttaataat attaagtata ttctaccatg ataatcaata
    20821 atgtcaatat atgaataaga ataattaata ttggacgata accaagcagg cgcttttgtt
    20881 ctgcttatga ctgggagagg cgttcgcctt tgaggaacca acctcccata cggttatccg
    20941 cgtctttagg ggcgcggtcg tagtagagat caaaaatttg ccctgaatcc accatcacgc
    21001 agaagtaaaa ccgccccaca ccccaagaac cagagcgcga agcgctggcc aggtgagccg
    21061 gcgccatatt gcgcgacatg cgcccgcggc gcgcgtaatc cacccgctct tccacgaccg
    21121 aggtgatggt aaacacctga ccacgccagc gaaagcgctg agggcagggc ggttttttat
    21181 caaaagtggg cggcgaatcg aattcgacct ggatgggctc atcgatgaat tgggccgggc
    21241 gggttttcat tcttcacctt tcacctggcg ctgatccatg gaaaaaccaa tcgcagaatg
    21301 tgtttgatta agtgggcgat ttgtcatcct cccagtcatg cggttcaatg tgcacatagg
    21361 cgcggtcgac ctcaggaaag gactcgagcc ttttcgtgac ttcatcggat atttcgtggg
    21421 cgtgatttac ggtgctgttg ccgtccacat tgatatgcaa atcgagcatc aaccgcggac
    21481 ccacataatc ggtgacgatg tggtgcacct tgatcacacc aggcacggat tcagcggcgg
    21541 tgacaaactg gttgattttc tcttgcgacg cgcccttacc ggtgagaaaa cccaggttct
    21601 cccgcccggc gaggaaggcg ttccgcagga tccaaacagc caccaggaac cccgccaccg
    21661 ggtctgccag ggggtgaata taattggaac ccagaacccc cacaaaggct gccagggaag
    21721 acaggacatc gctgagattg tcgcgcgcgc tggtggcgag cgcggggctg tgaagcctct
    21781 tgctgatgcg cgtgatcgat gcgtacatcg cggctttgac cagcgcgctt gccagcaata
    21841 cccatgtggg caaatccagg cggatggcgc tcagacccgg ctctgtgtaa cggacataag
    21901 cggtacgcgc tgcttcgatg cctgccaaag ccatcgcggc ggcaaccatt aaggcgacca
    21961 ggggttcaaa ccgtgaatgt ccctgaggat gactaagatc aggcgggcgt gtggcaaccc
    22021 acagacccag caccatcaat aacgagtaga ccacgtctga aaccgagttg gcggcatcag
    22081 aataaaccgc ggatgaacct gatagccagg ccgccacccc ctttcccacg gccagcagca
    22141 aattaccgcc aagtgtgatg ctcatggcga tacggtaata acgattttga tcaggctgtg
    22201 ggactgtctc tctcacccag cgcattcaac gtttctcctg ataatcaaca tcttgaagat
    22261 gcaatgcttt catccgtgat agggtttcca cctttatcgt taattcatgc tcatttccag
    22321 gctgtcagcc ctgcctggtt gttcgcattg agtaaaaaca catcccctgg tttttcaggc
    22381 tggctttgag gtcttaattg cagccgccgt ggccttcacc acagcgcgtt tcttcaaatt
    22441 gcagggtggt gtggtgaatg tcgtattcag cggcaagcag acttttgatc ttctccaaca
    22501 ccaatggttg ctgagagaaa aactccgccg agagtacaat atgggcgctc aacgccacct
    22561 ggttcgaacc caggttccac acatgcaggt cgtgaacagt atcgacctcg ccgacggagg
    22621 cgatggattg gtgtaattgc tgcaaagaaa gcccttcggg cacaccctca atcaatatat
    22681 gcagggattt gcgcagcagg cgataggcgc tgaccaggat gatggcgccg atgaggacgg
    22741 agattaacgg gtcgatccag cgttcgcccg tcagccggat gagcagcgcc gcgatgatga
    22801 caccgataga agaaatgagg tcgcccagca cgtgcaggta agcactatgc acattcaggt
    22861 tgcgtttttc gcgcttttct gcgtgtcctt catggtcgtg cccgtgctcg tgcgtatgat
    22921 cgtgcccgcc aagaatgaca gcgacgatga tgttcactac caggccaata acggcgatga
    22981 tcatcatctc cgtgctcttg atttccagcg gagcctgcca gcgtttgact gcttccaccc
    23041 agatgcctac ggcgatgacg agcaaggaag cgccattaat gagggcggcg atcacttcag
    23101 cgcggtgcca gccgtaagtg tgacgctcat ctggggggcg cgcaaccagg cgcagtgcga
    23161 agtagctcaa taccagtgcg aaaatatctg aaaacacatg cgcggcgtct gaaagcaacg
    23221 ccagcgagcc agacaataaa ccgccgataa tttcagcgac cagaataagg ccggtaagga
    23281 tgatcgaaat gataaaacgt tgttgggttt tactttctgt tttcgtgttc attgtgaact
    23341 ccgagcgcgt gcctcagcag gcaattttta tctcgcctac cgagttgaga atttgcgtgg
    23401 ggcggtaagg gaagcgctcc acatcctcac gggtggaaac accgctcaac accaggatgg
    23461 tctccagccc gctcatcacg cccgcgacaa tatctgtgtc catgcgatcg ccaatcatga
    23521 cggtatcttc agaatgcacg ccgaggtaac gcagggcgct gcgcatcatc agtgggttag
    23581 gtttgcctac aaaaaaaggc tctctgccgg tggctttttg aatgagcgca gccagcgcgc
    23641 cggtggcggg cacaataccg gcttccagcg ggccggttga atccggattg gtggcgatga
    23701 acatcgcccc atcattgatc aggcgaaccg ccttggtgat caattcaaaa ttgtaattgg
    23761 tcacttcacc cagcaccacg taatcagggc tgaggtcggt ttgcacatac ccgacgtcgt
    23821 gcagcgcttg cgtgagcccg ctttcaccaa tgacgaaagc cttgccgttg ggcttctggc
    23881 tttgcaagaa acgcgcggtg gcgatcgccg aggtgaaaat ccgctcagcc ggcacgttga
    23941 gaccgatcgt attgaggcgg tgctcgaggt cgcgttgcgt atagagcggg ttattggtga
    24001 ggatcaagaa tttcactttg caggcgagca ggcggtcgat aaattccacg gcgccgggga
    24061 gtggggtctt cccggttacc agcacgccgt ccatatcgat aagatagttc ttcttcacgc
    24121 gcattcctct tttcattgac gggtacattt taacacggat gaccgggttg ttgtattttg
    24181 caaagaaagc ctggtgtgta aatcggttat aatcaacgcg ttggttccaa caagaagagg
    24241 taaaccagtg ccacggcggc gcgcgattga agattacaca gccagcgaac tcaggcagct
    24301 tctcatggag aagagccgcg tggaacgtga agccaggctg gaagcgttca ggaaagccgg
    24361 caggctgatc gaggctcctc tgcctgctga gggagaaaca cctgagccgg caatcgaggc
    24421 tgaggagtat accgcctccc gcgcccgccg caccgccagg cggcggcgca ctggtcttga
    24481 tcgcttcctc ttcgcggtgg aaatcatcgc catcattggc ttgctcttta tcattttcaa
    24541 tggggtgaag ctcatgcgcg agctgaacca gcaggtggca tcggtcattg agcaacccac
    24601 gctgaccccc acgccgctga tcaccgcggt ggtgttgcca ggagggcatg tgcctccagt
    24661 atccgggcag gagaaccgct ttaacgaagc agaaatccct gaacacctgc gcccgctgat
    24721 gcagagttac gccaacctac ccgtgcccac gcaaagccct caacaggcgc gtcagataca
    24781 gatccccgcg attggtgtta gcgcaccggt ggtgttggga gatggctggg agcagttgaa
    24841 gaagggcgtg ggacagcata ttggcacggc caaccccgga gataagggca acctggtgct
    24901 ttcggcgcat aacgatatct tcagcgagat cttccgcgac ctggacaagt tgaagaaagg
    24961 tgacgaggta accattatca cacaggatag gtccttcacc tacgtcatca ccagcagcca
    25021 gattgtggaa cctactcgag ttgatctgct ggcgcagacc agcgacagca tgatcacatt
    25081 gatctcctgc tatccgtacc ttgtggataa caagcgaatc gtggtgcagg ggattttgca
    25141 gtgattataa ttacgtaaca ggagaataaa atggcaaaaa aggttaaaaa acgctcaaca
    25201 aacgcacctg ccgcggtggt ggcaaaaacg gaaacagcaa cccctaaggc tgcagcatcg
    25261 cgaacgccaa ccgcagaatt cgaccccgat tactcttttg tcaagaaaga cctcaagcgc
    25321 attggcattc tggccgggtc cttttttgtg gttttggtgg tgctgtcttt ctttctgcgt
    25381 taagagaact acccggaagg gcaacttccg gatccaataa accaacttaa taaggagaag
    25441 cgtatgaccc gtatgttcgt accaggacca gttgatgtgg cgccggaggt gctggaagcc
    25501 caaaccaaac cgatgcttcc acaccgcagc aaagattttg aagcaatttt tcgtgggaca
    25561 gaagaaaaag ccaggcagtt attctatacc cagttccgcg tataccaggc ggcatcatcg
    25621 ggttcaggta tgcaagaagc gggtatccgc aattttgtgc aaaaagacgt gctctcctgt
    25681 attaacggtg ctttcgcgca gcgctggtac gatgtggccg tagccaacgg gaaaaccgcc
    25741 gataaactgg aagttgaata tggcgaggcc atcactcctg aactgctgcg tgacgcctta
    25801 aaacagaagc attacgaagc cgtcaccatt gtgcataatg aaacttccac tggcgttgag
    25861 aacccactgg cggacctgtg cgcggtggtg aaagagatca gccccgatac gctcattttg
    25921 gtggacgcgg tctcttcgct gggcggcgtg aaaattgaga tggacaaact gggcgtggat
    25981 tttttgctga ccagttcgca gaaatgcctg gctctcccgc ccgggctggc gctggcagcc
    26041 gccacagacc gcgcgctggc aaaagcagaa aatgtgagca accgtggctg gtattttgac
    26101 ctgctgttga tggaaaaaca ccgcgtcaaa gactccaccc ccatgacccc ggcgatggcg
    26161 cttatctttg ccctggaccg ccagctcgac cgtatcctgg cggaggggct tgacgcccgc
    26221 ttcgcccgcc acgcggccat ggccaggagg gtgcaggaat gggcgagtgg gcatggcatg
    26281 gagccgctgg caaaacccca gtaccgctcc aagaccgtgg tgaccatcaa aaatagcctg
    26341 aattgggata tcagcgcgct caacaagttc ctccaaacca ggggcatgcg cattgccaac
    26401 gggtacggca agatcaaaga cctgacttac cgcgtggcca ccatgggcga aaccagcatg
    26461 gcggatatcg aggctttgct cgtggcgatg gaagattata tgaagcagag ctaaccctat
    26521 acaggaaacc gaaaaggctg ggcgaccggc ctttttttcg cgcgcctggg cagcctggaa
    26581 agcctggagc gaaagcgcgg aaaatgattg accattgtca acgggcgtga taaaatcttt
    26641 gcgtttgagg ttgggcgcgt agctcaatgg cagagcagcg gccttttaag ccgttggttg
    26701 agggttcgat cccctccgcg ctcactnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26761 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26821 nnnnnnacct ggaacagccc cagcaggatc aggcccaatg aaataaagct ggtattccct
    26881 ccggctgctt cggggttgct ggggaaaaga aaatacagca cagcgccgcc cacagcgaac
    26941 aacgcgccgg tgatcatcat cttgcgcgcg ctttctttgg tcacctgcgg tttggcgcgc
    27001 agcctggcgc ctgacttgat gtacccctgc acgccctctc ccgtctccgc gcgataccaa
    27061 ctctcttcat tcacggtttc cactccgcgc acggtgaaca gggtcttttt ggtgatggtc
    27121 gcgagcaccg gtgaagaatc atcaggctgc tgatacagct ccaggtcatt gccgacggct
    27181 tccacttgct gaatggcgaa gatgtgcgtg tcgcccgaga tgtagccggt aacaccatta
    27241 tcgagagtga cggtgaccca aacctctttc tttttgcgga tcaccttgcc tatttcaaat
    27301 tcttcgccct tgtggataga agtcaaagaa atggattcgc tgttgggttc ttgccagacg
    27361 cgcgcgcttt catcgcctaa gcttgctctc atatttattc tccttgaaaa actccgggtg
    27421 tgtattctgc tgtttgaaaa tggtaatagg cagcgcactc cgccgcccag tcatcatcgt
    27481 gaaggttgat ttccagcaca atcgctgacg gatcgctttt atcttccggc acaggcgtgt
    27541 aaattttcgc ccgcgggcgc gcatccaaag agacgccagc cgattccagc tcctcgatca
    27601 atttacgcgt gcggtatcgc tggtgcagcc aaacggcgag gatgatcgcg ccgcctaaac
    27661 caacgatgta tggccaatag gcttttaccc cgccaagcgc tgaatcagcg gggatgaacc
    27721 gggtcatcaa caacataccg gcgcaggcga ccacgccgcc aacggtgtaa aggcggctgt
    27781 aaattcgcgc cagccggcct gcgctggtat catctttttc catttcttct acactggtgg
    27841 cgaagtcggc gcggtagcaa gtttgacaga cccgtatttt caggcggtat ggtcgatcct
    27901 ccttgtaggt ctgtcgatat aatcccgggc gtttgcgccc gatgcgcaac ctcaggctgt
    27961 gagtttcgta atcttcagcg ggaacagcgc cgcagcggga acagcgcttc gggaacgcca
    28021 gcaccaggct gctgttaagc gcctgttgca cacgttcaag cggaaccatt atcttaagcg
    28081 gcatcgttgg tctttatggg cgcgcaggtt tccatcacca cacaattgta cctgaaaagt
    28141 cgtttggcag ccccatttca ccgcctgaag aatgttgttt caagaacctc taacctgggc
    28201 acgatatatt ccagcaaggc gcgggtatct gcttctggcg atttcttctt accctggcga
    28261 tgaccacctt gcctggctgg caggcgagcg gtcttcccaa tccggcgagg tggctggtca
    28321 tccttcaata accggtcacc tggttaacac ttctaaaaca attacactgg agcccgcggt
    28381 gcaaaaggtt ttgcggtctg cgggtttgat aatgaagaca cttggcttgg cggttgataa
    28441 taccaatttg cctttgccgc ctaccagggg gattgtctgc aattgcccat tgacctcaag
    28501 ttccacactt ggcaggtcaa gcgtggtgct tacatcaatg acgaccgcgg ggtcgccttg
    28561 atgggcttcg accgtaaagc aagcgctgcc gttgtgcgac agcaccaggc ggcgcgaata
    28621 ctctgccatt gattgttcaa cgaaggcgag ggtttctccc ttctccgcgt tgtaagggaa
    28681 tgatgtttcc ccgtatgtga gcagatggcc ttctttatcc ttcaacacgt atggcattca
    28741 tgacctctca ttaaacccag gcgaatatgc ggtaataacc tgaacccgcg ttccccgcgg
    28801 agcaggcgat cgcaccggct gaagcgtggt taaagttctt gatatataca ttggtggcgt
    28861 cccaatagac gatgtcgatc acgttcgtcc cgttcgaggc tgatataggc ccacaccggt
    28921 aagttgaatt gtcattcgtg gcgctataaa aagcgatgca ctcgcggggg aactgaccca
    28981 ggccgtgcgc tttggcgtat gtattattgt atgaacaagc gaaccagccg ctatcatagg
    29041 cagggagcct tgtatccccg ccgtttgaat aagagcgaat ggagagcggg gcgatcgctg
    29101 tgccattcca gtaacactcg ccgatcaact tctggtccgc accgggcacg ggcgaggtat
    29161 taactgtcag tgtgaaggag gtaacccccg gggtgcggtt ggcgaatata taccagactg
    29221 ctgctccccc cgagaattgg gcggcgggaa gatcaatatt ggcggtctga accaacatac
    29281 agccagaaat catcacggac gcgggctttt gtgaatcaaa aggcacgcgc aggcgggcaa
    29341 ccgccaggta ttcaatgttc atattcaata cgaaagactg caggaaggtg gcgaggttta
    29401 cgctatccgc gctcacattg ccgagataca cagcgtcttt gcgcagattg ttgtagtggg
    29461 ctgcagcggt gggttgcccg ctggccacat ctgaactgtt aggataaggc attggcttac
    29521 ctccatttga aaaatttcag acccagtagg aagtctccca agtaagagga ccatcccaaa
    29581 cagatgcggc gatcttctcg gggtctgcgg gggggaaata aaaacctacg ttattgagct
    29641 gaacgcggat gatggaatgc ttgggcaggt ggcgttcatc cgcgctgaag ccaagcgcgc
    29701 cgaggttggt gaacgttccg actggcgtgg tgcattggat acctgaaggc ttgctgagaa
    29761 agtccagcag cttggcgtag tcttgcaggc gggtgggcgt gtgctcggct ccgtttggtt
    29821 cgaggagatc atcagcgcag atgatatcaa agttgatcca caagcgcgtg ccggtaacaa
    29881 tgtcagtata cgtggttgac tcgctctcca cctggcggat gaactgtgca ccgcattccc
    29941 ttaccgttcc gtctttgggt gatacgaacc cgtatggagc gccggagtcg atctccgcgt
    30001 gggataggat gacgtgacaa ttttggccca gggtgtaggt gctcatctac ccgccttgtc
    30061 ccaccggtca agcggatagc ccagcggaaa accgaactcc tgcatcactt ttaatccgtt
    30121 tagcgctcgt tcaaaggttg cccgccacag gtggctgatg ctcatcagcc gggctgattc
    30181 gcgcgggtcg ctgccgtatc gccctaaaag gctgccggcg cgcagcaggc aaacctgggc
    30241 tgctgcgcca tccaccagca caccatccag ggcaggcggc aggctggtgc tgggggagcc
    30301 gtccaaaccc tcgatcgtgt aacccgcggc gtagtggatg gttaacccct cacccgcggc
    30361 ggggatgtat tgcccgagga agtgcagtgt tggcgtgccg ttgagaagca ggtaggtaaa
    30421 acgagtctct ggttgcaact cgcgggtggt ttcgccctcc ccgtgcgcgc tcacgctgag
    30481 gatgtagcgg caatcgctga tagagaggat gggttgctgc ctgccgctgg aagtgaccgt
    30541 gaaggggctg ctgaccaggc gcgggagtcg ttgttccagt tcttccagcg tctggcgcgt
    30601 tgccgcggcg agcaggtcat cgctgaagcg ctgttcgctg ttatcttcca acagcgcccg
    30661 caggcgggtt ttaatgtcgt cgaactttgt catggatttt ttcctcaagg tgcttcaggt
    30721 cgggcggcag cacgatcatt tctgacttgc cctcgcgggt gccggctgga gggcgggaac
    30781 tgatggcgga agcgcggctt tttgttggtt tcacgggcgg caaacctgga gggtgcgcgg
    30841 gcttcatccc cagcgcttca cgggcgctgt tggcttcctc cagagtgaac cacagtttgc
    30901 gcccgtcagc gctgatgatc accatgccgc catcaggcag gctgcgccag gagagcgggc
    30961 gactgccctg cccgggtctg tgataaggcg ctgccaggtg ctgcgccagt ttgagctcag
    31021 ggctgtcggc tggcattacc gctgtcctcg atagccacac cggttgagta gacggccagc
    31081 gccgccacca gcgcctgcag tgcctgccac acgtcgatct gctgcgtaag gtaggctgcc
    31141 agcgtggcga tgattgctgc cattagcgcc caaaacttgc gtgagcgcag catcattttc
    31201 agttgttcaa ggaaagtcat tttattctcc cgttttcgtt cgcgcagggg cgtactgcgc
    31261 ttttactgat gattggctga tgccaacagc acacccctgc gattaaaagc atcaggccac
    31321 gttggcttta tacaatgggc gataatctgc cacaaacacg ctcacccagt gacgcacttt
    31381 catgcggatc tcatcgttgg taaagagagc cccgttggct ccgctgtcgg cgatgatgat
    31441 ttcaggcagc accccgaagc gctcacccag gatgatgcct ggcgccaggc gcgggtcggc
    31501 gagagccgcc cagtcgttgg catcgctgaa ctcagggcag gtgatcacat ctcccatctg
    31561 ccccttttgc atgttctctg caaaaatatt gctttcatgc gcgaaagaag ggtagaggat
    31621 gttcatgccg gtcaggcgca ggtcgcgcgg taccagtaaa taacgggcgt cgagcgccag
    31681 ctttggcgcg ctgccgccgc tggccactgc catcggctgg ttgtagatgg cctttcccgc
    31741 cgcctcccac gaggcggaag acagggcgct ggtacccagg ttggcgtgat gcgcgctttc
    31801 gaacacgttg taagtgtctg acatcaccgg gccgaccccg ttgttgctgg tgaagatggc
    31861 gccgacgagg gcggagatgc ggcgcagcgc ggctgaagcc agtttgcgcg gatactggcg
    31921 taatttgtgc gtctcgtcgc gctcgaacat ctccagcgtc aaacccacat aaccgccgta
    31981 tttgccccat gaaccggttt cagcgctgtc ttttaccgcc agctcggtgt acgccgcgcc
    32041 ttctgaaaca gagggcagca cggtgacctc tcctaccagc acgccggtta tctcctgcag
    32101 gttgttaaaa tgttccacct gcaccaaggg ttcccaccag cggtaaccgg agcgtcccag
    32161 ctcctgccat tgatcgaaga tcagtttgtt catggcattt ttaagcagcc cgggcaggtt
    32221 cgatgttgcg gcgaattggg cgtgttcacg gtgataaccg ccagtgaaac ccatatcgcc
    32281 agtcatcagc gtgtagagct cgcggatgcc gctcaatcgc gccggctgca gtccctccag
    32341 ctcacgcggg cgcgcagccc ctaaaaggtc atgcagcgcc gcgttcacct ggtcttcagg
    32401 gttggcgata acgctcacct gccccgcgcc ctggataacg ctgcctgcgg tgaggtcgct
    32461 gataagcgcg cgtgtatcac tgatggcctg ttgcagcgcg gcggcttcaa aaatgctgcc
    32521 gctgaagcgc gaccgcaggc tgttttcagc ggcagccggc aggcgggccg cggaaagcga
    32581 aatttccagc aggctggcgc atagttgccg ctgtgtttcc tggtgagagc tcgcgccggg
    32641 cgcgtccaca ccggttgccg gattatgatt tgactcttcc atgtgcttct ccttggtttg
    32701 gaatagttgc tgttcccggt tcaagccggg ttgatcgttt acgggtaaaa actcaccgcc
    32761 gcgtgccggg tgcatcacca ggtccactga gagcaccttt tcaatgcgta tcacctgcct
    32821 gccggcagcg ctgaacagca ggtcagcgga gaaccccacg cgcggagagg gttgctcgcc
    32881 cgcgtacatg gcggctgccg tttctttcag caacgcggcg ccaggcccaa ccgggcgcag
    32941 gttgagcagc accccagagc gcgtgtcgca ccaacgcggg tcacggcata caccggccag
    33001 gtcgcgcagc gagcgctctt tccaatcgtg gtcgataaaa cattccgcgc cttcccacag
    33061 gggcagcgaa tcacgcagca catccgcggt aaactgccaa ccgttgccgt tgccagcgct
    33121 gatggcgatg atttcaaggc tgtcttctgc cacagcgcgc agctcagtgg caaaatgcgc
    33181 gcgctgctgc tctgcaacgc tctgcctggc gtcagtcatc tcttcctcct ttttcttccc
    33241 cgatggcggg gtcaattttg acgggctgcg tttgagcaat tgcccaattg aaaccctttg
    33301 ctggcgctgc ctgccttccc cgtgccagca tttcatccac atccacggtt tcgccgcaaa
    33361 aacggtagaa aaggcgcagc aattcggcgt cgtccagcaa gccacgttca cgcaggctgc
    33421 tgagcgccgg cataatattg ccggctgcca tcgccagcga caggttgtcg cgggcggaaa
    33481 tatccgcacc gttcaactgt atttcagcgc ggcgagaaac gcgccggtcg accactgaac
    33541 gcctgttcac aaccacgcgc agcacatctc ccagcagcca gaggaaataa cgctgacgtt
    33601 gctcaaaatg gcggtaagtg ggtccgccgg cggcttcagc ggtggtgcgc gtggtgcttt
    33661 ctggttctgc caaaaagtgc agcggaacgc ccgcgccggc tgccagcatc ttttttaacg
    33721 acagtccatc ctggccggat tcttcagact ccaaccgtgg gtgcagcgtg tcccaatgct
    33781 cgttctcgtc agtcaccagt atggaacccg gcgaaggcgg cgcggcgtta agcgccgact
    33841 ggcgcgcgcg gcgttcgacc tcactggtaa aacgcgcgcg cacaatgtac agaaaggcgt
    33901 tgcggaaatg gttgagccgg gcgcggtcct ccagccaggc ggcgtagcgg ctgagccagc
    33961 gcagcaacgg tgccaggtca gattctcccc actgggcgcc gaccgcgcgg ttgatggcga
    34021 aatgcagcat cactgtttca aaacgaccat cctcgcgcgg ttggtcgtgg agggcgtcgt
    34081 atgccggcca gcgcgcccct tcctgtccct ctgcgaacag cggctgcatt tcgtaggcgg
    34141 tctcctggtc gatatcgttt gcgtgtgcgt ggatggcgcg cacacgcagc gcgggcacag
    34201 cccgcaggta gctcatgcct gacgcgtcag tgaagagcag taaaaagagg ttgccgctgc
    34261 gcgtcagttc gtcgcaccat tcatataccc gcaccggcat gcggttgagc gggtgctgcc
    34321 agaaggtgtg caggaaagcg ctggtaggct cgtgcgcgca agaaaaactg atgccgccgc
    34381 ccaccacgta ctgcgaggtg agccccacga tgcgccgcgc caggggattt acccgccagg
    34441 cttccagcgc ctggcgcagc agttcctcgc ggtctggcgc gtaacgctca cccgggttct
    34501 cgcccagccg gctgccaatg gcgaaggtgg cgtctgtttc agggaatgcg ctgagggcgg
    34561 cgcgcacacg ttcgttgatg ctgcgccgga agaggcgctc gattaagccg ctgctcatga
    34621 ttctaccagc gcggaaaggt ccgctgccgt accgctgaac aggttcaggt caacacgcgt
    34681 gctgataccc gcgatctgcc cggcgctggt gtactgccag aacgcccagc cagcccaggg
    34741 atacacctgg caaggccagc cgctggtgta atgcgccacc cacaacgggt gctctgccgc
    34801 ccagtcaacc cctgaacgca gtaactggta atttgaaagc accggcaccg gcagagagct
    34861 cttccagaag cccggggaga catacagcat ggcggtacga ccgctgagtc tgaaaacctc
    34921 ctctgtaaat actttgagcg cctggttgaa accgcttttg cccaatcctg gggtttcgat
    34981 gtccacgcag ggcggcaggg ttgcctggcc tcccgccagt tcttgaagcg tgcgcaggta
    35041 atgccgcgcc tgcgccagcg ggtcataacg cggcaaaaag aaatggtaac taccggtgac
    35101 gatctcggcg gcggcggcgc cacgcatgtt ctgctcgaac atcgggtcga tgtagccctc
    35161 gccttcactg gctttgatga tggcgaagcg cacgccgctg cttctcactt tttgccagtc
    35221 gatccaatcc tggtggtggc tgacgtcaat accaaataac ataagtaagt cctttcgtga
    35281 atgagagcca aggccggaag cctgataagc gcccagcgct caaatggcgc ccgggtcatt
    35341 gatctcaact gacgattgtt aaaatttccc ctccatccca gtcagcgggt cgctgccggg
    35401 gatgatgaag gagggcgagg taggtgacca gtccaggttt tccaggcagg cgcacaacgc
    35461 cgcggagagc acccaatcat catgaactgg ctcgccgctg gcggtgtcac gcgttccgtc
    35521 tggtacgccc cagcgcaggc gctggtccgg gccgctgccc acctggaatt gactggctgc
    35581 aagctgggcg aagaattgcg cctgccagcg tgcctgctct ggctgacgtt caccgtccag
    35641 ctgtggctca cgccagcgcc ctgaatccac cacgccgagg taatcccagc cgagtttgga
    35701 tttggaggcg gcgttgaagg tgaaaggcag cacgcgcccc ggcagtgagc gttcgaggaa
    35761 agaactcaac ccggcgccga tgccagtggc gtctaccacc acaacccgcg cctgccagct
    35821 caacgccagc gcgcgcacct cgctgtgcag gcgggtgtga ttcactcccg tccactgctg
    35881 gcgcagcacc gggatgtaac acggcagcgg aacgccttct gtggcgggtt caacgcgcac
    35941 gatggtgagc gcgttactgt cgcggctcgg gttggatgag gcaggggcgc catcgctggc
    36001 gcgggcgact gcgtcttctc ccgccacatc cagcagcagc gcataatggc tgccgggcag
    36061 gggttcggct tcggcgcttt cagcagaacg catacgcgcc aggcggtcag gtgggaacat
    36121 accgccttcg ccgtctacct cttcggagta gtactgtgtg cgcacagcgg gatgagccct
    36181 gcccaggcgt tccaccgttt cttccacgtg gtggcggtag gcagcgacct cggcagccac
    36241 atcatccgcg gtgagccgga aaacgcgccg tagaccatca cgcttttcag cctctcgggc
    36301 gcggcgtagc tgctgcgcca gcagcgtgcg gctggtccag gcggtgcccc aaaaaacgcg
    36361 cgtggcgttg gtggaggcgc tcatcggcgc cacatctttt tcatatttaa cgcacagaac
    36421 gtcttgcgct tcatccacct ccaacaatgc gctggcagtg gcgccgacga tgttgctctc
    36481 aggcgcggca gaaaggaagg tgatgcgcgc ttcgccgata cggtaagtgg aaccggattc
    36541 tttaacccaa atgcgctggg tgagcttgtt cttttttacc gtacgttcga ggcggcgcat
    36601 ggcagtgagt gcctgaggtt tcagcgtggg cgagactttc accatctcca ccgggaagtt
    36661 ggaaaacagg gtcagcaggt aggtctcgag ctgcgcctgc agttcgttct tgccgctctg
    36721 gcgcggaaaa atgaccacca agctgaggcc gcgttcgttg agcactgaat caaagatggc
    36781 gctgagcacg ccgcgctggt atttgcgcag cgtgatcccc gaagccatct gggtgaagcg
    36841 ttcgggttct cttagaagcc atttgtagtg cgggatgaag ttttgcacgc agtcctcagt
    36901 tgaatggcga aaatttggcg cttcaggcac atcacgcccc gcttttcacc tgttcttgaa
    36961 cttctgccca aacttcttta actctggcca ctcctcctcc aactccagca atgcctgccg
    37021 cagcagggcg gcggggtcga ggtccaggtt gttgaggtcg cggcgggctt tcaacaggcg
    37081 cgccagcgcg ggagcggtct tgccgaccgt gtcgagcagc tcgagctgct cctccaacga
    37141 cagatcatcg ctttgtttgg cagcagcctt gtggatgagc tcgcgcaaga tgtcaatctc
    37201 gcggtccagc ccttcagggg tggtcaggtc gcgggtggtt tgcactccgc gcgtgggtgt
    37261 ggttgcggtc tttttctgtg tcatactcct ccatatagaa ataatgttct atttcgttag
    37321 ccctatcatc ccacgcccgg cgcactttgt caacttgccc caggggaaaa tttttaagca
    37381 aaaacttgcc agaatggcaa gtttttgggg aaaatgaatg gagtgcgccg cttcaggcgc
    37441 aggttctttt ctgttttaca ctccttttac gcaaggcaaa aaccagtcgt tgacgcgccg
    37501 ggaagggaat tcagctgatt ccgatgaggc agcgcgttcg ccgccgcgtt attgcttgcg
    37561 cggggttcag gaggtgaaag aagccagcag ccagcgcgcc agcaggctga tgaggatggc
    37621 gtataaaaag ctggcgaagg tgcctatgat gatgtactcc gcttgcttac ggttctcact
    37681 gtctttcagt tcaccgaagc gcaggatgga tttggcggtg ataagaaaac ccaccccggc
    37741 gaactgcccg ctgagaatga agacgaagat caacagccgc tccaggtagc cgatatacct
    37801 gccgccttct ttcaaccctg ataccgggcg caggctgttg ggacggaact ttttattgag
    37861 ctgcgcttga aaaggttgca ggaaccagcc gatgagccaa ccgcccagca gcgtgagcag
    37921 caacgcgcct gccaaaacaa tgaccacctg cagggcggag agcgccggag cgggcatggt
    37981 cgccataggg agtaaaagcg tcagcagtgt caggtggatc aaagttacct ccgagagatg
    38041 agcttaccaa gtcgctgttt catacacggg taatggcgtc ttcatagaag atcaggcttc
    38101 ttttgatggt tggccaggcg gaacggctga tattgttgga aacagacggc tgacggatgg
    38161 gcgccgggtg ccagcgctgg gcgatctggg tttgcgtcag cccttgcagc gcccaataaa
    38221 ccgcctggca ttggctggct ctccacgagg tcaccagggt gtccaccagt tccaccagga
    38281 tgaccgcggc attgtgtgcc aggctgtctt ccggcgccgc gaaccccagc gccattcgct
    38341 ggctgcccga aagcgtttcc aacaggtgac cagatgctgt gtacgccgaa ccgtagccgg
    38401 cggagacgtt attctcgggg atgaactcca cctcaccgat accgatggcg atgcgcgagt
    38461 cgagcttcct ttcgctgacc gcgaaacgga ggtaagtgcg gatgtacaaa ctgatttcga
    38521 gcgatttctc agggcgggcg atgatcaatt gccagctgtc gccgcgaaaa ttgctgatgt
    38581 cgtgacgcac agcctgtggg tagcgctcgc gcagcatggc agacaaccgg gggaaggttt
    38641 cgaacagttt ctgccgatcg cgcggcaaaa gattggagga gttaacgata tctccggtta
    38701 atacggcgtg ttccagtgag acgtttgtgt tcatcggatg gacctgcggt tgtgtttctg
    38761 atgctcatta taacagatta taggcgaaaa ggctatacag tcaaaatata gagtgaaaga
    38821 ctataaataa cgtatatagc cgaccagtct atatcaaacg tctgcgccgc cgcaaggcgc
    38881 tcctgactac cacacgcccc acgccaacca tgcgccggcg tcgacattaa taaggcaggg
    38941 gagcgtctgc acaggtgtcg gcgcctattt ctggcagaat gagcggtgtt tttcagggtt
    39001 tcttatacgg atatctggac aaaatgccct aaaaggtgta tagttcgcca ccgaactaat
    39061 taattgaaag agggaagaat tgacggaatc tcaaggtaaa ccacaacgca tgcgcaggct
    39121 gctggaagag atggccgccg gtgaggatat ccaggggatg gaaattgccc gcggcatccg
    39181 cgtgctcaac cgcctgtatg acctggcggt cagccgttca gcggagttcg gagagctttc
    39241 cggcgcgcgg ctgggcatcc tgctgcgcct gctgatggaa gaagagtgcg gcaatacggc
    39301 gggggtgaac cccacccgtc tcagccattt ccaggatgtg aagaaaaaca ctatcacctc
    39361 cctgctcaaa gggctggagg aaagcgggct cattgaacgc gcgcccgacc ccgcggatcg
    39421 ccgcgccgcg ctggtgcgca tcagcccggc agggcgcgag ctggttcgtt ccaccgcgcc
    39481 agcgcgcttc gcttttttga accgagccgc cgccggcctt gacgcgggcg aacgccgcca
    39541 gctgctggag ttgctgaata aactacgcac ctccctgcag ccgcacgctt gctgccgacc
    39601 ttctgtggaa gattgaatca atcgaaaagg aacctgtacc tgtatgttgc gcatctttcg
    39661 ttaccttaaa ccttacaccc tttccgtgct ggtggtcatc ctactgctgt ttgggcaggc
    39721 catggctgag ctgtcgctgc ccaattacat gtccaagatt gtcaatatcg gtatccagca
    39781 gggcggaatc gaaaacgctg tgccggaggt gctgcgctcc agccagcttg agcgtatgct
    39841 gctgttcctc acccctgatg aacaggcggc tgtgcgcgct gcttacaccc cgcttgagcc
    39901 ctcctcgcct gcgtatgacc ggcttatccg gcgctttccc gctgccgtca gcggagaaac
    39961 gctcgcccgt gttgacctga cgcctgagca gatcgaaacg ctgaacccgc tcagcggacg
    40021 cgcgctgctg gcagtatcca tgctggaaca ggcagccagc gacccggcaa aggccgccgc
    40081 cctcagccaa caaagcggta tggacctcag ccgcctgccc gcaggcgcag atgtgttcgc
    40141 cctgctacat caactgcccc cagagcggct gcagcagatg gtttcaattg tcaggggtcg
    40201 gtttgacgcg ctcgacagta gcatgatcac ccaggcggcc gccgccgcgg taaaaaccga
    40261 gtacatcgcg cttggcgctg acgcgcaaaa gctgcagacg gcttacatcc tgcgtattgg
    40321 gggaaagatg ctgctgctca cgctgatggt ggcgctggcg gtcatcatca tcagcctgct
    40381 ttcggcgcgc accggggctg ggtttgcgcg cgacctgcgc agcgacatct ttagcaaggt
    40441 cgcgagtttt tccaaggctg agttcgagag cttttccacc gcttcgctta ttacgcgctc
    40501 caccaatgat gtgacccaga tgcaaatggt caccactatg ggcatgcgca tgctcttctt
    40561 cgcccctatg atggcggtgg gcgccatcat ccgcgcggtt gaaaagagcc cttcgatgac
    40621 ctggatcatc gcgctgggag tgggaacgct gctggtgatg gtcattacca ttttttctgt
    40681 ttcattgccg cgttttcaac gtatccaaac gctgatagac cgcctgaatc tggtgacgcg
    40741 tgagaacctt tctggcatca tggtcatccg cgcctttaat tcacaggaat tcgaactcaa
    40801 acgctttgat aaagctaata tagacctgac ggaaaattcg ctcttcgtcg gacgcgtgat
    40861 ggtgacgctc ttcccgctga tgactctcat catgagcggg ctatcactgc tcatcatctg
    40921 ggtgggcagc caccaggtgg cagcatcagc cttgcaggtt ggcgatatga tggctttctt
    40981 gcaatatgcc atgcaggtgg tcttttcttt cttgatgatt tccatgatgt tcatcttcct
    41041 cccccgcgcc gctgtttcag gacgccgtat tgacgaggtg ctgcgcgttg aacctgctat
    41101 taaagacccc cctcagccca aaccctttga cagcaattcg cgcggtctgg tcgagtttcg
    41161 taatgtttca ttccgctacc ccaatgcaga agagaacgtc ttgtgcaaca tcagctttac
    41221 cgccaggcct ggagagacca ccgccattct cggcggtacc ggttctggta aatccaccct
    41281 ggtcaacctc atcccgcgct tctttgacgt gagcgaaggc gagatctacg tagacggcgt
    41341 ggatgtacgc gaggtcgcgc aagcagacct gcggcagcgc attggttaca tcccgcagca
    41401 cggtgtgctt tttacgggca cggttgccag caacctgcgt tatggcaatg agaacgccac
    41461 agcagaagaa ttggcgcaag ccgcgcagac cgcccaggcg gctgatttca tcgctgagat
    41521 ggaaggcggt atggatgccc ccatcgcgca gggcggcacg aacgtttcag gcgggcagaa
    41581 gcagcggctg gcgatcgccc gcgcgttgct caagcacccg cccatctata tatttgacga
    41641 cgccctatct gccctggact ataaaacaga tgcccggctg cgtcgcgcgc tggcgaatga
    41701 aaccaacaga agcaccatgc tgattgtgac gcagcgcgtt tccacgatca agcacgccga
    41761 gcagatcgtg gtgctggatg atggcgagat tgttggcaag ggtacgcatg atcaattgat
    41821 gcagagctgc gagacttacc gcgaaatggt ggcttctcag ctcagcggag aggagttgct
    41881 gcgatgagcc aggcgaacac tccctccagg caaaccatgc ccttacgcac tcacggccca
    41941 ggcggcggcc cccccggcat gttgcgcggc ggtgaaaagg cgcgcgattt taaaggcacg
    42001 atgaaaaagc tggtgcgcta cctcggcccc tataaattcc acttcctcgt tgtgctgctt
    42061 ctggcggtcg cctccacggt gttcaatatc gttggaccaa agatcctcgg cagggcaacc
    42121 accaaggtct ttgaaggcgt ggtgagcacg atcgccggta caggcagcgg gattgatttt
    42181 gtgggaatcg gcaacatcct gcttttgttg gcggggcttt atatcgcggc tgcaggtttt
    42241 ggctttatgc agggatggat catgactgcg gtctcgatga agctaactta caacttccgc
    42301 cgagaaatct ccgagaagat caaccgtctg ccactgcggt attttgattc caccagccag
    42361 ggcgaggtgc tttcacgcgt taccaacgat gtggatacca tcagccaaac catgaaccaa
    42421 agcctgaccc aaatcatcac ctcggtgatt accctggcag gcgtgctggt gatgatgatc
    42481 tccatcagct ggatgatgac gctggtggta ctggtcattc tgcctacctc agccgttttc
    42541 atccgcgtgc tggtgaagta ttcgcagaaa tactacaaac aacagcaaga ctatctgggg
    42601 catgtaaacg ggcacgtgga agaaatgtac ggcgggcaca ttgtggtgaa agcctttaat
    42661 ggtgaaaagc gcagcctgga ggaattcaac cgcctgaaca gcactctcta tcactccgcc
    42721 tggaaatcac agtttgcctc gggtttgatg atgccgatta tgaactttgt cggtaatctc
    42781 gggtatgtag gggtggcggt gcttggcggc gtgctcgcct tccgcggagt gattactgtc
    42841 ggtgatatcc aggctttcat ccagtacgtg cgcaatttta cccagccgct tacccaaatt
    42901 gccaacatct ccactgtgct gcagcaaacc gcggcggctt ccgaacgcgt gttcgagttt
    42961 ctgtctgaag ccgaagaaat acctgacact gacacgccag tgcaactgcc tgcagtgcgg
    43021 gggcatgtcg aatttcgcga cgtgcgcttt gggtatgtcc ctgaaaagac catcattcac
    43081 aacttctctg cggagataat gcccggtcag aagattgcga tcgtcggtcc taccggcgct
    43141 ggcaagacca ccatggtgaa actgctaatg cgttactatg atatccaggg gggcgcgata
    43201 ctggtggacg ggcatgacat ccgcgagttc agccgagagg ggctgcgcga cctgttcggc
    43261 atggtgctgc aggatgcctg gctgtataac gcatccatca tggagaacat tcgctatggt
    43321 caactggatg ccacggatga agaggtgtac caggctgccc gcacggccaa tgtggaccac
    43381 ttcatacgca ccctgccaga agggtacaac atggtgctgg atgaagagac caccaatatt
    43441 tctcaggggc agaaacaact gctgaccatc gcccgcgctg tgctctctcg accgagcatc
    43501 ctcatccttg atgaggctac ctcatctgta gacacgcgca ccgaggtgct tatccagaaa
    43561 gccatggaca atctcatgcg cggtcgcacc tctttcatca tcgcccaccg cctatccacc
    43621 atccgcaacg ccgaccggat tttggtgatg aacaacggcg atattgtgga gcagggcacc
    43681 caccaggaac tgctcgccag aaatggtttt tacgcttcgc tgtacaacag ccagtttgaa
    43741 gaagccgaaa tctactgacg cttgcgaaac ccaaagcccc cgcatccggc gggggctttg
    43801 aggtttgata aagcagccat cctcagcgga ggatgagcac gtatgcccgc tcgattgcag
    43861 ggtcaagcgc gcccaattct ctctcccaca agcggttgga gatgtggcat aatcactggc
    43921 ataacgggtt tttaaggaaa tttcaatgga gatcaatcac atctgtgtca ttggcctggg
    43981 ttacatcggt ctgccgaccg ccagcatgtt cgcccaaaat ggcgtcaggg ttaccggcgt
    44041 ggacgccaac ccgcgcgtgg tggagagctt acaacagggc aaagtgcaca tccatgaacc
    44101 cgggttgacg gaagtggtta caacggtgtt acgcgatggc acgctggcca ttgcttcagc
    44161 tcctgttgag gctgacgcct tcatcatcgc cgtgcccacg ccattttatg atgataagaa
    44221 agcagacctc agccacgttc gcgctgctgc cgaatcgctg gtgccggtgc tgcgccgtgg
    44281 aaacctggtg gtgctggaat ccacctcacc gccgctgacc acacagaatg tgttaagacc
    44341 cattctggag caatccgggt tgaatgcagg ggaagatttt ttactggctt attcgcctga
    44401 acgggtctta ccgggtcaga ttatgaaaga actggtggag aacgcccgcg tcatcggcgg
    44461 catcaacccg gcttcagcgc aggctggtaa agacctgtat ctcaagtttg ttcgtggcga
    44521 agttgtcctc acagacgcgg cgacttctga gatggtcaag ttgatggaaa acacattccg
    44581 tgatgtgaac attgccgttg ccaatgagtt ttcacgcctg gcggagcgtt tgggcgtaga
    44641 tgtttgggaa gccatccgta ttgccaaccg ccacccgcgc gtgcgcatcc tcaaccccgg
    44701 tcctggtgtg ggcggtcact gtatcagcgt cgacccatgg ttcctggtgg aatccgcacc
    44761 ggatatcacc catctgattc gcgcggcgcg cgaggtgaac gatactcaac cggagtttgt
    44821 gctccaatca cttaaaacaa tattaccgag cccgcaagga aaacgcgtgg cagtgctcgg
    44881 tttggcttat aaaccggacg tggatgacct gcgggaaacc ccctcgctgg aagtggtgcg
    44941 cgagctggta ttggcaggcg ctacagtgac agcctgggaa ccatacaaac cagacttcaa
    45001 ggctcccaat atgaagatgg cagccagcct ggaggaagcc attcaggacg ctgatgtatt
    45061 gttgctactg gttggtcaca gccagttcaa agccctggac cctgaggtgg tggcgcaaaa
    45121 aaccagcgcg cgcctggttc tggacgcagt aaatgcctgg cccagccaac cctggcagga
    45181 cgccgggttt acattgaaaa aactgggcaa cgggaaacct ttgtaatgat gaccccgttg
    45241 cgcgtactct ccatttttgg cactcgcccc gaggcggtga agatggcgcc ggtggtgcag
    45301 gcgctcaaga gccagcgcgg cgttgaatct cgtgtgtgtg ttaccgcgca gcaccgcgaa
    45361 atgctcgacc aggtgctggc cttgtttcat atccaaccag accatgacct ggacctgatg
    45421 cgccccgacc aatcactggt tgaactcacc tctggcatcc ttacccgact ggacccggta
    45481 ctggaaaagg agcgccccga ttgggtgctg gtgcagggag acacgaccac ggtgatggca
    45541 gccgcgctgg cggcttttta ccgcggcatc cgggtggggc atgtggaggc cggtctgcgc
    45601 accggtgata aacgccagcc gtttcctgag gagatcaacc gccaccttac cacggtgctg
    45661 gctgatttac attttgcccc caccgaacgc agccgcctta acctgctgca cgaaggggta
    45721 ccggaggcac acatcagagt gaccggcaac acggtcattg atgccttgca gcagattatc
    45781 cgcactgacc cgccggcgca ggtacgcgaa tggctggatg agtggggcgt acgccccggc
    45841 ggtagaaggc tggcgctggt cacggcgcac cgccgtgaaa acttcggcag cccgatcgag
    45901 catatttgcg aggcgctaaa gaccctcagc catcgatacg gcaatgagct gcacattgtg
    45961 tacccggtgc atctcaaccc gaatatccag gaaccggtgt atcggctgct ggcggggatc
    46021 ccaaaagtta gccttatcaa gccggtagat tacatttctc tcatccattt gattaagaac
    46081 gcgtacattg tccttactga ttccggcggc atccaggagg aagcggcagg cttgggtaaa
    46141 cccacactgg ttctgcgcga agtgacagaa cgccctgagg gtgttgaagc aggcgtgctc
    46201 aggctggtgg gttcaaacaa ggatcgtatt gtgcgtgaag ctgcgcggct gctggataac
    46261 cccgacgcat acacggaaat ggcgcgtacg accagccttt ttggtgatgg gaaggcggcg
    46321 ggcaggattg tggaagcgct cctcaaccaa ccgaaacgac ccgcttaggt taaaaaaggg
    46381 gaaagtgtga acctgtttca gccatgtaaa atttgcatac taccgcgcaa actggggttg
    46441 ggcggaccgg cgtcttttca gataggcttg gtcgacgcca tgcagcggcg cggtatcacg
    46501 gtgtgccatg acccgcttga aacaggcgtt gccgctatcc tggtctttgg cgcggcacaa
    46561 aacacaggcg cattaaccct cgctcagcgc gccggggtac gtgtggtgca gcgcctgaac
    46621 ggcatgaact ggatccatcg ccgccggttt accggcgtgc ggcatttcgt aaaagccgaa
    46681 tatggtaact tgaaactgcg cagattgcgt aaaagcgctg accgcgttat ttatcagagc
    46741 gccttttcac gctcatggtg ggaacgggaa aacagcgtac tggataaacc tcagtttgtt
    46801 attcacaatg gtgtagacct gggttacttc tcgccccatc cggccaacct tccacacgac
    46861 cgttatcgct tgctgatggt agagggtcac catggtggtg gttacgacca gggattgctc
    46921 accgcggcgc gcctgctgga aggtttgcgc tgcgtggctg accgtgattg tgaattggtt
    46981 gtggtgggag atgtgccgac gaacttaaaa caacaaatca cctcgattac cgcgccaatc
    47041 agctggttgg gggtggttga gcgcgcaaag ataccggaga tcgaccgttc agcgcacctg
    47101 ttgttttcag gagatatcaa cgccagttgc cccaactcgt tgatcgaagc gatggcttgc
    47161 ggtttgcctg tggtcgctta tgctaccggc gcgcttcctg agctcgtcag cgaagaggcc
    47221 ggtcgtttgg cggcgtatgg aggtgatgtg tggaagcttg acccgccgga tatttccgca
    47281 ttgacagacg ctgcctggca ggcgttgcag ggcagggatg agttgagccg cggcgccagg
    47341 ttacgggtgg aagcgaactt tgatattgaa aaaatcgctg aaaagtatat agaagtgctg
    47401 ttgggggatt gagcgaagaa gaaactcagc gaaccctttt actgttgcgt gtatacaaca
    47461 atgtgccgat accgacaaca agcaggatgg tgatcaccgc aaggatcacc ccgtcggtca
    47521 agccatactc tgatgaaaga gggcgttcgg tggcggccac agtggcattg agggttaaag
    47581 tcacgggcgt aggcgtggcc agcgggtcga attcatcccc cggctttggc ccgcttaaga
    47641 gcatcgcgcc ggccagcagc gccagcacaa acagcaacaa caacagcctg cccgcgacac
    47701 caggttcttt gaataatttt tgccactgct tgcgcaacat acttctcacg ttctcattcc
    47761 agtggtacct cagccagtga tttgatgatc ttattggctg attcgatata agtggtttgc
    47821 tgccgtatca tctcgctagt ggcctgggcg aatagcgggc caaccctgga aatgatgtgg
    47881 gcaggcggaa ctacacgcgc gctttcggcc aactcaagaa gtatacctga tacttcgttg
    47941 ttcttccata aactcaacgc cgtccgactc accggcaaca ggcccgcgct ttctgtccat
    48001 tcagcctgga accggggttc caccaggtaa gccgcaagct gacggtaaag ctcgaagtgc
    48061 tcaggcgagg aagccgggaa ggcgatgacc cacccgtcag cgagcgacag gcgtttttca
    48121 gagaaagacg ggacggaagt gatggcgatg ttttccggcc gggctgaaag gtattgcgaa
    48181 gcccgtacga ccgcgtaggt cgcttgcgca tccagtacgg cttgccacga atcctcaaaa
    48241 cggttgctgt tcgtcagcca gtatggaaac acatctgcat ttgccccttc agccagaaac
    48301 atcaaggttt gctgcagcgg cacttcatct actgtgattt gcgtctcgtt ctgtgtgaag
    48361 gttccgcctt tttccagata caggagctgg gtggtcaacc cctgggcttc gccagccggg
    48421 aaggccagta tcttgcctaa ctggatcacc tcctgccaat aaacgggtgg gtaggccacc
    48481 tgctggtttt tgtacgccag gcagaaaaca tccgccgcaa aaggcaggcc atagtccagc
    48541 ccattcacac gaaccaggtc ttcagcgaag gcaaaatgat ctgcctggtt tgcctggttc
    48601 tgttcggctt caactggcac gatcaatccc tgactgaccg caatggccag gtcagcatgc
    48661 gggaggatga gcaagcccgg tacgactgcc ggggctgcca gggtggcggc gctgagagaa
    48721 tccagcaggc tgcccggccc gctaagcgct ttaacccgga cctcaatggt gacctcggga
    48781 tggtcatagc gaaaggcctg aagccgttcc tggatgcgcc gccccgcgga cgagccgttt
    48841 tcagggtcga aagcaggggg cagccagaag atgattttat ccaacttatc ttccactacc
    48901 gcaggcagct ctgttttttg catatcgtga gtcaaagtcg cctgagggga cgcttccggc
    48961 tgggtcgcca gcgctgtgcg ccgcgggaac ggtatctcat tcagtagttg ttggcagccc
    49021 gtggctgtaa taaaaaaaac cagggcaaac aacagggtgg ctgacctggc gaggagtctt
    49081 ttcatttttt ctccagcttg atctcccggc tcaataaccc tgtcagcagg gtgagcgcgt
    49141 gcgcataatc atccatgtcg atcatttcgg aaggtgaatg cacgtagcgg caggggattg
    49201 aaagcgcgcc tgatggcatc ccccctcttg aaatttgcat cgcgcgggca tccgtagagc
    49261 cgacatccag cacttcaagc tggtgaggaa ccttcagttc cctggcggtc ctgaccatcc
    49321 agttcaccac ggccgggtca gcgatatgcc caacatctcg caccttgatg gcgggtcctt
    49381 ccccaaggtt cacctgcatc ttcaccccca gaatatcgcc tacaggggtg acatccagcg
    49441 caatgcctaa atcggcatcc agctgataag cggctgtggt cgctccacgc gagcccactt
    49501 cttcctgagt gcaaaaaacg aagagcagct cgtgcggtga ttgttttacc ctctggatgg
    49561 tttcgatcat caaggcgcag ccacagcggt cgtccagcgc tttggaactg acccgtttgc
    49621 ccagatccag aaattcgcgg tcaaaaacgg ccaggtcgcc gattttgacc gggcaatcct
    49681 tttcactgat ggcgccgatg tcaataaaga actggctgat gggttggatt ttcgaaacct
    49741 catcatgtcg atctgagttg atcacgccac gcgtgccatt aaggaatacc acacgcccac
    49801 cggcgaggta gcggggcaag acgccgccga ggttgctgaa cctcaccagc cccttctttt
    49861 caatgtggct gacgatgagg ccaatttcat ccatatgcgc ggcgaccatc acccgcatac
    49921 cgcctggcgc ctttgtgccc acctgggcga tgaggttgcc catggcgtct acgcgcgttt
    49981 ctcgcacgta cggtttgatg atttctcgaa tggtctcgcg gatggcggat tcatagccag
    50041 acggtccggg tatctgtgta agttgcttga tcaactcttt cataagtgtt cctcttgtgc
    50101 gttgggttta tcggtcggca ttgagaatac tgcgatcggc tttttcgagc gcctggtaaa
    50161 gcaacgccag ggtgtttttc caatcgctca atctggcgtg caggatcgga gaatgagcgt
    50221 atcttcccgg aactgaaact gaaacgcttg gcacgccacc tcgggttatg tgaatggcgc
    50281 ctgcatcggt acctccgcct ccaggttgcc ggaactggta agggattcca cagtcttcgg
    50341 caatgcgcgt caggaactgg ataaggcgcg ggtctgaaat agtgcccgcg tctgccaggt
    50401 agatggccgg gccagcacca agccggcagt tatacagcac gttctcatct tcgctttcat
    50461 ctaacggcag gtcgaaggcc ggggttgaat cgaccacaaa cgccatatcg ggctcaaagt
    50521 cgtaagcggc aacacgcgct ccacgtaacc ccagttcctc ttgcacggtg aaggcgaata
    50581 aaacttccgc tgttccgggg ttcagcctgg cgagttcaat caaggttgct acacccagcc
    50641 ggtcatccag cgctttgcca aagaggctgt cgccgttgcg ccagaagcgc gtggcgaaag
    50701 tagcgtaatc accgacactt gctttgccgg cgcaggcagg gctgagatca attcgcagtt
    50761 gccccagcgg ggtgacttta gcgcgctcag cagggctggt gaggtgaacc ggtttactgc
    50821 cgatcactcc gggcagatgg tctttaccga ccagcacagg ctttgccagc agctgccgtt
    50881 cgtcgatccc gccaaccgct tcgaaggcgt agcacccttc agtgtctgcc tgcaccagca
    50941 taaaaccgac ttcgtccata tgcgcggcga acatcacccg agcagcatct gctgttttgg
    51001 tgtgttttat ggccagcagg ttgcccatgc ggtcaacccg gtattcgtcg accaatgggc
    51061 ggatttcttc aatcactatt tgacggactt catcttcgtt tcctgacaca ccaacagcgt
    51121 tgctcaactt ttcgagcagt ttcaactgag ggcgagaaat tttaaccctg ttctcattca
    51181 cgccgcacct cctttgaaga gtgactccag gctgcccgag gtgagagttg taataaaacg
    51241 ggcgagcaaa cgcccggcgc gatgaatgtc tttgagggcg accatttcgt atggagtgtg
    51301 catgtaccgg agggggattc caatcaccag ggtgggtact ccagcggaac tgatctgcat
    51361 cgccatggca tcggtgcctg agcctttcgg catcgtttca atactgtaag gcaaatcgat
    51421 ttcttctgcc cggtctttca attcccggtg caacgctggg tggatattgc cgcctgcgcc
    51481 gatggttacc cccttaccga gcgggaaagt ttcgtaagtt gataaccccg gttcctgggc
    51541 aaaggtcaca tcaacagcga taccaacatc gggtttgagc tcgaacgtcg aggtgt
//
LOCUS       DAOTLW010000009        50508 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_817_length_50508_cov_10.996036, whole genome shotgun sequence.
ACCESSION   DAOTLW010000009 DAOTLW010000000
VERSION     DAOTLW010000009.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 50508)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50508)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50508
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_817_length_50508_cov_10.996036"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(41..658)
                     /gene="tsf"
                     /locus_tag="P5516_02630"
     CDS             complement(41..658)
                     /gene="tsf"
                     /locus_tag="P5516_02630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HRT91366.1"
                     /translation="MEITTEMIKSLRESTGAGILDCRKALEQSNGDMNAALDFLREKG
                     LATATKRAARTASEGIVELYNHGDGRVGVMVEVNCETDFVGRSEAFRTLAHEIALQIA
                     ATSPEYVLETDIPESVIDRETKIATAKAKEEGKPEAILPRIVEGALKKYKNEFVLMLQ
                     PYIRDESITVQELINQNVAALGENIVVRRFARWALGETSAEESES"
     gene            complement(714..1925)
                     /gene="rpsB"
                     /locus_tag="P5516_02635"
     CDS             complement(714..1925)
                     /gene="rpsB"
                     /locus_tag="P5516_02635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HRT91367.1"
                     /translation="MSSIISMKSLLESGVHFGHRTNKWNPLMRPYIFTERNGIHIIDL
                     QQTVKLLTTAYNLVRDTVAQGGFIMFVGTKRQAQETITEEAVRCGMPYVTERWLGGML
                     TNWSTMYSRIQELEKLERLRDSGEIHRLTKKEGLLIEREINRLQARLSGVREMKRVPD
                     LLFIVDVMREHTAVHEANLKGIPVIALVDTNCDPRHVDYVIPSNDDAIRAIKLLVGKI
                     ADAVIEGKSMVEKEESSEAPAAETPAARVAAGVRPRFEEEVELEDADLLGASTLAKIG
                     PKADAAKEDEDLDMKPGVGKSVIAEETLDVLEEEVDKTVSVKSKSAKKSDDEDAAAAA
                     EEEKSTAKGKHARTAKEIVKNIGDFSEDLVDNIKDKIEDTVDDLKDKAEDLIDEIKDK
                     VEDIVDKTDGE"
     gene            complement(2226..3059)
                     /locus_tag="P5516_02640"
     CDS             complement(2226..3059)
                     /locus_tag="P5516_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo alpha-1,4 polygalactosaminidase"
                     /protein_id="HRT91368.1"
                     /translation="MLKTISRTWILLLLLSGCGVQSTNISPTVYPSSTLSTNVRWWRP
                     TPGLTWQLQFSALPVEMGVEADVYDIDLFENDASVVEALHKQGRKVICYLSVGSREDW
                     RPDAGQFPGEVIGKSYIGWPGERWLDIRRIDLLAPIMRARLDQCKAKGFDGVEPDNIE
                     VFTQDSGFPITYADQLAYAQWLAAEAHARGLAIGLKNAPGMVADSLMFFDFAIIESAF
                     FYEWIEETLPFIEAGKPVFAVEYTDTGVDFQAACDWSRENQISMIQKDRMLTSALIVC
                     P"
     gene            3177..3959
                     /gene="surE"
                     /locus_tag="P5516_02645"
     CDS             3177..3959
                     /gene="surE"
                     /locus_tag="P5516_02645"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HRT91369.1"
                     /translation="MHILVTNDDGVQAPGLLALATELSSLGEVTILAPDRNWSASGHV
                     KTMHRPLRVKPVLLADGTPALSSDGAPSDCVALALLGVVEKPIDLVVSGINPNANVGH
                     DVTYSGTVTAAMEAAIGGVRGMAVSLDAPEHHKGALDYAAAARVAGFVVNRLLQNTLP
                     VNVLLNVNVPYLKYEEIKGIRVSRQGLRIYRDALIRREDPRGRPYYWIGGEAPTGVPR
                     EGTDFGDLQAGYVSVTPLHLDLTAHEQMAHMQSWDWSADEIR"
     gene            4069..4491
                     /locus_tag="P5516_02650"
     CDS             4069..4491
                     /locus_tag="P5516_02650"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRT91370.1"
                     /translation="MDKTTVKDWMKNLVVFVDPDTTVFEGLHIMRHRFATSLIVKKTA
                     ENPDYGIVTSINICDKIIAQGNDPTKTKVKEIMASPVFTVPSSMLITDCAAMMKEKRI
                     HHLPVADEHGQIVGIITAMDLLWMAEAMGTNFKDRGLS"
     gene            4559..5515
                     /locus_tag="P5516_02655"
     CDS             4559..5515
                     /locus_tag="P5516_02655"
                     /inference="COORDINATES: protein motif:HMM:NF012415.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HRT91371.1"
                     /translation="MFIRVALLGGLLLSLPQGTAQAATVSPGELINLVNGLRAANGLP
                     ALIEDAILDGTAYATAQAMAANGVCAHIGGARERIRAAGFGGGAVVFATENMACAVSA
                     TISDIQSWWSDSLHMMPMNEARYTHVGAGAYTASNGTTYYVLHAAYVGDGGSASLPGS
                     TGGSAGQGQNPAEPLVEPVVTSTPQADGTIVHTVQYGQALDTIATWYGVTIAEIKALN
                     HMTSNTIYVGDNLIIKIAPTATITPTRTLTPRQPTRTPSMTPTLTKTVMVSTPTPTRA
                     PGLLDTLPKIDRQWLGFGLLLVSAAGLLLVLYFLFIRPRFKK"
     gene            5840..6415
                     /locus_tag="P5516_02660"
     CDS             5840..6415
                     /locus_tag="P5516_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91372.1"
                     /translation="MEKRNRVTLVLGLLLVLAGTYFVLINAVPGLAQLITVHFSWPVI
                     IMLVGAGLLILGLLSGVAEMSIPAFIVAGIGGILYYQNLSGNWSSWSYLWTLIPGFSG
                     LGVLVAALFGARKKGAARDGLDAIGTSLVLLVIFGAIFGAFKPLGPYWPVLLIAAGLV
                     IGARAFIKGCKEETPVNVIDVKPSGEADENM"
     gene            6424..7065
                     /locus_tag="P5516_02665"
     CDS             6424..7065
                     /locus_tag="P5516_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91373.1"
                     /translation="MSQQSRRILLGVLLVVAGAAFLLQQLLEIPIGGLFIAFLFVLAG
                     CVFLYFLLRDRHKWWLAIPGFTLIGIGGVILIKKLLPNLAGDYGGAFFLLMVALGFLV
                     VFLLNQAQWWPVIPAGILTTLALVSALDLNGMANGGLLFLGIGATFTVLGLMPVGKAE
                     KWPWIPAGICLLLGLILFASSGALMGSAVGWVFALVLIAAGAYFVIRSLIKKA"
     gene            7076..7531
                     /locus_tag="P5516_02670"
     CDS             7076..7531
                     /locus_tag="P5516_02670"
                     /inference="COORDINATES: protein motif:HMM:NF015957.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HRT91374.1"
                     /translation="MQNRLLRSKQDRILGGVCAGLARYLNIDTVLVRLFFIVFTLVGG
                     IGPLLYIILWIVVPEDQAESSSGYSFNGEVLRERAENFADEFVNAVQKPGNKASFYVG
                     IGLVLLGVYIFLKNLNLPWLAWLNTSVILALLIIIAGAALLYVALKRKT"
     gene            7619..8338
                     /locus_tag="P5516_02675"
     CDS             7619..8338
                     /locus_tag="P5516_02675"
                     /inference="COORDINATES: protein motif:HMM:NF016300.2"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HRT91375.1"
                     /translation="MKKSLLVIVASIMAVMSLAACAGSGQPAGMPNLRTLSASGSGEV
                     YLVPDVAYINVGIRVDADEVSDALSKNNAQANSISEVLQQLGVDKKDVQTSNFNVYPM
                     MDYGIDGQVSRRYYVVENTVYITVRDLTSLGKLLDAVVRSGANTINGISFDVLDKTAA
                     EAKARDMAIANAKAEAEAIAASAGVKLGNLQSVNVYTGGIAVPMYDAKGIGGAAMDSV
                     VPISAGQLKINVEASVIYEIK"
     gene            complement(8402..9391)
                     /gene="glpX"
                     /locus_tag="P5516_02680"
     CDS             complement(8402..9391)
                     /gene="glpX"
                     /locus_tag="P5516_02680"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HRT91376.1"
                     /translation="MPNTTPTRNLAFDLVRVTEAAALAAGRMMGRGDKNGADKAAVDA
                     MRVMLNSISMRGTIIIGEGEKDEAPMLYNGEKLGTGEGPEVDIAVDPIEGTRPLAYGL
                     WNSISTVALAPRGTMFNPGPFVYMNKIAVGPVARHAVDIDAPVSDNLHKVAKAKGVKV
                     EDLTVVVLDRPRHQELITQIRMAGARIKLIPDGDVAGALMTSMGDNTADLLMGVGGTP
                     EGVLAAAGLRCMGGEIIGKLYPRNQRELDLAKEMGVDFEKPLTMDDLISSDNVFFAAT
                     GITTGDYLRGVTYFKDGARTDSVVMRGLSGTVRRVISTHKLSKLMRISSIKYK"
     gene            complement(9422..10246)
                     /locus_tag="P5516_02685"
     CDS             complement(9422..10246)
                     /locus_tag="P5516_02685"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HRT91377.1"
                     /translation="MKTDLSLRKRLIVINLMVPTLIVFSILGGWAFTVFVAVVLLLAA
                     WELWRLFNKGQYSPSLLVMGIFVPLAVVLRHLYAFSYSDVWLAALVLTSMLFHVLQQN
                     RGGMTAAIDLMITIGGTVYLGWLGSYAISLRALENGLHWVVLVFPIISLADTGAYIFG
                     RLLGKHKMLPRVSPKKTWEGYLGGILVGMLGGWALAALWHKPAPLILPWYGLVLGAVI
                     SILAPFGDFGESMLKRQFNAKDSGNFLLEHGGFLDRMDSSLWAAAIGYYVIILLTR"
     gene            complement(10236..10961)
                     /locus_tag="P5516_02690"
     CDS             complement(10236..10961)
                     /locus_tag="P5516_02690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HRT91378.1"
                     /translation="MTKKTPAKIPAHIAIIMDGNGRWAKAHGLPRLEGHRAGTENLRQ
                     IIKGCAEIGVQHLTLYAFSTENWSRPKTEVDGLLTILETYLDKEVDELCAEGVRLHHI
                     GRLEAMPESVRKKVEHSMEVTCSNTRLILHLAWNYGGRDEIVHAIQKMIRDGIPSKDV
                     TEELVDRYLFTAGTPDPDLIIRTSGEMRVSNFLIWQGAYAEWYVTPTLWPDFTKEELF
                     KAIEIYDQRDRRFGNISSSNNEN"
     gene            complement(10979..11536)
                     /gene="frr"
                     /locus_tag="P5516_02695"
     CDS             complement(10979..11536)
                     /gene="frr"
                     /locus_tag="P5516_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HRT91379.1"
                     /translation="MLKEITKDAENRMKSALLSLEEDLTAIRTGRATPALVEKLPIDY
                     YGAPTPLLQLASISVPEARALLIKPFDPSTIKTIERSILASELGLTPNNDGKSIRLNL
                     PPLTEERRRELVKVVNNRLEECRVAIRNVRRDLIKDLRDFEKEKMISKDDLETGEQDL
                     QKLTDLMIDEIELIGKRKESEIMEI"
     gene            11748..11981
                     /locus_tag="P5516_02700"
     CDS             11748..11981
                     /locus_tag="P5516_02700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91380.1"
                     /translation="MNTGMLWFDNDPKADFLTKINRATDYYLKKYGQRPDLCFVNPGM
                     KFEPPPKNVGIEVQTDQMIMPDHFWLGIKTCSA"
     gene            complement(12026..12964)
                     /locus_tag="P5516_02705"
     CDS             complement(12026..12964)
                     /locus_tag="P5516_02705"
                     /inference="COORDINATES: protein motif:HMM:NF024622.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HRT91381.1"
                     /translation="MMTEEDIDSGRLILQSRHDFSHAYQKGFWRSVFHLIQKKNNELL
                     PFEEVLKHIPMRGQNYIGVRQIETEKIIGSVSRYNDFDRAFLPRQTHTRSRWESIDRA
                     HFKQIILPPIEVYKVGDFYFVKDGNHRVSVAREKGQVYVDALVTEVHTSGKITRDTDV
                     NALVLEQEYSEFLRQTRLNEFYPGEDFRFSIPGQYEKVLQHISVHRWFMGEKLTRPVT
                     DEEAVKGWHREVYLPLVRIIRKHQILNAFPARTEMDLYFWIIEHRWYLAEEQKRRISL
                     ESAATDFTNRFSQRPFRHVSQLWQRLKKRIFNQEKK"
     gene            complement(12968..13627)
                     /locus_tag="P5516_02710"
     CDS             complement(12968..13627)
                     /locus_tag="P5516_02710"
                     /inference="COORDINATES: protein motif:HMM:NF012376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HRT91382.1"
                     /translation="MKILAVSDVENALLYSPMIMDRFQDVDLILSCGDLPYYYIEYMV
                     SMLNVPCYFVRGNHANAREYGVAGTRKYPWGAVDVHHRCVEDDTGLLLAGLEGSLRYN
                     QGPYQYTQGQYWQMVLSLVPRFFWNKLALGRYLDIFMAHAPPWQVHDEEDLPHRGIKA
                     FRWLIKVFQPAYFLHGHIHIYRRETITRTLIGGTTVQNVYGYKTLHIPAPRPRSWRGQ
                     R"
     gene            complement(13643..14020)
                     /gene="acpS"
                     /locus_tag="P5516_02715"
     CDS             complement(13643..14020)
                     /gene="acpS"
                     /locus_tag="P5516_02715"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HRT91383.1"
                     /translation="MMEVACGIDLVEVERLRSITPAIKARFVSRVFTTQEIEDSGGRD
                     DRLAARFAIKEATVKALGCGIGEVGWQDIETQLDENGKPVLRLYGAAAQMAERMNWRS
                     WSVSISHTRNLAAACVTALVEKR"
     gene            complement(14017..15321)
                     /locus_tag="P5516_02720"
     CDS             complement(14017..15321)
                     /locus_tag="P5516_02720"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRT91384.1"
                     /translation="MIPKYTLSSSIPGPADITRQVLRNGIVLLTRSNFNSSSVVISGY
                     VSAGSQLDPLEKLGLAHFTALALMRGTESSDFQSLYDRLESAGASLGFGASVHTTSFS
                     GRALAEDLPLLFATLAECLSSPVFPSEQVSRLRAQLLTGLAIRGQSTRDTAALEFDSL
                     LFPDHPYGRPEDGYTETIQNITRDDLADFHRQQYGPEGMKLAVVGAVSADRVFELAEK
                     HFGNWLTGHKRQTCSFKAIKPPQASITRKVIIPGKSQTDLIIGTLGPKRKDPEYLAAS
                     LGNSILGQFGLMGRVGDAVREKAGLAYYASASLNAWIESGSWEVSAGVSPGNVEKATA
                     LIIEELRRFTTEPVSQEELEDNQNNYIGRMPLSLESNAGVANSILNLERFELGLDYLQ
                     RYPTLVSALTREHILAAARKFINPDKLIIVSAGADGKVNNNP"
     gene            complement(15318..16604)
                     /locus_tag="P5516_02725"
     CDS             complement(15318..16604)
                     /locus_tag="P5516_02725"
                     /inference="COORDINATES: protein motif:HMM:NF017044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HRT91385.1"
                     /translation="MTETITQVELENGLKVLMKEIHTAPIISQWVWYRVGSRFEKPGF
                     TGISHWVEHMQFKGTAKFPAGVLDKAISRDGGYWNAFTYLDWTTFFETTPANKIDLAM
                     TLEADRMVNSLFDENEVNSERTVVISEREGNENEPLFLLDEAVQFAAFDHHPYKNEVI
                     GSIADLRSITRDDLYAHYRKYYNPGNAVLALAGDFDSEKILERIRQTYQPLHGHSVPE
                     PEYLREQLLAEEKRIEVAGPGETTYLQVAWRSPAATEAEFFAFTIIDSLLTGPTSLNM
                     FGGGGISNKTSRLYRRLVEKEYAISLNGSLQATHDPFLYTITITVHPKHQPEVVLEQL
                     DKELDVLRQAPVKQTDIDRAAKQARALFSYGSENITNQAFWLGYSEMFDQYSWFTRYT
                     SRLAEVTPEILLDTARKYLNQNQRVVGIYRPDGAKR"
     gene            16739..18067
                     /locus_tag="P5516_02730"
     CDS             16739..18067
                     /locus_tag="P5516_02730"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT91386.1"
                     /translation="MSKRIQVAIIGAGPAGLFAAEKLTQADIAVALFNRDIKPGGMAE
                     YGIYPEKHQLKDGLRKQFERILSYEQVHYFGNTCVGEDQSLTIPRLLEWGFSAVLICC
                     GAQGTKWLGIPGENLEGVIHSKNLVFHYNRLPPYCTAPIKIGKQAVVVGAGNVMADVT
                     RYLLGLPQMEKIHVCVRRGPAEVKFTAKELESIIGGMDMDALEHEFERVKPVMVPLGQ
                     DPEVEKAVYLKAFGKAGKTGLKPLIEMHFLVSPVKIEGDEQGGVNTVVLEENTLVLSG
                     EKTSARGLGTHRSLAADTVVFAIGDRVEERLGVPVSGNEYRHVENPRYPVEGQSYEVE
                     APGSGQAWKGVFVAGWSRLASSGLVGNAKKDGVNGAEAVEQYLREERPGGGLLLDELE
                     SRLSSLPDPVVQQRHLARLLAEERKRAGGGPIEYAKFTTNAEMLQVMGLQ"
     gene            complement(18078..18563)
                     /locus_tag="P5516_02735"
     CDS             complement(18078..18563)
                     /locus_tag="P5516_02735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91387.1"
                     /translation="MTENSAIENEGKPKVTANSLPALPLARIHFALLVMLVGFIIFLV
                     GARPAIFGLDRSPVVGFIQIAVMLIGIMIICLGGYTSVRALWRHQPPSISADIGMRLV
                     STGYVIVTFSGLADVFGVGTHPLPGVPYFGVWQARGMEIGCAIIAIGFILSFPPASKP
                     Q"
     gene            complement(18701..19966)
                     /locus_tag="P5516_02740"
     CDS             complement(18701..19966)
                     /locus_tag="P5516_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRT91388.1"
                     /translation="MDQNVTHNGSGANLDNNQNDNNMASLLEKEGLGIDFPTQGEIRK
                     GIIASITPGQILVSVGTKSEGIITGKEYELIPSDELANLKVGDEISVYVVNPEDQNGN
                     LVLSYMRAREEESWQEVEALQASKQATHSTILGYNKGGLIVPIGGLRGFVPASQISLT
                     RRAGVTGDTPEQRWGKMIGQEIDVCVIEVDRERRRLILSERQASTETRESVKERVLEE
                     LKEGEVYLGRITSLADFGVFVNVNGADGLVHMSEISWERIQHPSEILKVGQEVKVKVI
                     NIDHEKKRIGLSIRQLQSDPWDQKAAQFQVGQLIEGTITRLTKFGAFARLAEDIEGLI
                     HISEISERRIEHPKEVLKEGDICTLRIIKIDPSVHRIGLSLRRVDSMAYADMDWQALE
                     GILDEDTAVIAKDDDQSTAEEPTEPAPEA"
     gene            complement(20121..24167)
                     /locus_tag="P5516_02745"
     CDS             complement(20121..24167)
                     /locus_tag="P5516_02745"
                     /inference="COORDINATES: protein motif:HMM:NF019156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91389.1"
                     /translation="MVLQKIARVFGGDPHKREIEHLSQIVDEINQLETVFEGLSSDAL
                     NHKTSEFKQRLAEGESLDDLLPEAFAAVREASKRTLGLRHYDIQMIGGMTLHHGKIAE
                     MRTGEGKTLVATLPLYLNALTGGSVHLVTVNDYLARRDARWMAPVYNLLGLKVGVLQM
                     AARTENGRKAFLVDLEKPNAREDLHMLALVDRGEAYKADIVYGTNSEFGFDYLRDNLT
                     MSLEERVQRGHYYAIVDEVDNILIDEARTPLIISGPASDETEDYVRMAQVVRALNPED
                     IEVNEKDHQVSLTEIGDAHVEEILNQPLRNPDRPEDIAPEQARLLGYLEQALRAQHLF
                     RRNKDYIVQGGKVIIVDEFTGRLMPGRRWSEGLHQAVEAKEGVKVEPENVTYATITIQ
                     NYFRMYEKLAGMTGTALTEAEEFSKIYKLEVLPIPMNLEYVAAQQDSPLTVVETRDRQ
                     NYPLQYYSKKDDPHHEAVFWRRQDFPDLVYRTEEGKLRAIVAEIIHYYAIGRPQLVGT
                     TSVEHSERLSQRLSAESVRRLAQTLVIRDAWLEKFNKSAEIEITELQFLNRPLHELNV
                     AEMRPLARQAGLSSINPEDPDLLERLLKVMDLEAPHKERVLEVLKGGVPHQVLNARKH
                     DEESQIIAKAGAFGSVTIATNMAGRGVDIKLGGELNEEVLADVIRVLEKAGVADPYDL
                     NNDTRRGELEKLTLEDYGIYEESVNTFIRYLEDMKRVRALGGLHVIGSERHEARRIDN
                     QLRGRAARQGDPGSSRFYLSLEDELMRLFGGAQMEGLMTRMKVDPSLPIESGLLGRMV
                     EQAQERVEGNNFDVRKHLLEYDDVLNSQRKRIYAERNRAFEKESLHEDVLEILRTEIN
                     ERIPAALKDEEGPWKLLSYLEEVQPSMVFEAEGIRTPSYTIRLIVDELQRRINGQTDD
                     SNIKDQIIEITEKALQAENEHILNATSLLLQKTEETIDQLTAERLESVDAIFDGLGSS
                     HGEEEAPRRPQEILEELVNLTRTQLRLTTDEMRALPEGDDEVKEKVNNQVKRALNQLA
                     VMRTLGAVERRLGESLNLKAADLQDQDWTSISDTLMEQVENLLTKRTASLLSAQGQVI
                     QNLEGALSKSPRGSSDENRLVDLLLNTSIGSKVAIDPRTHQRGMRRVILVNYIFYAAR
                     LIQDRPVTEITSEILQHLENTIERLETVWGEIEFERFRLSGSSMSQLDAGLQQSLRAE
                     VGDAAFLSLQTAQPDDLSAEARTAVEKTLGRRVQNQIYRHILLSVISELWVDYLTRVD
                     ALRVSVGLEAYAQRDPLVQYKGQASELFKNLLSDIRGGVISRMFLFQPRRPGGQPAER
                     SAPEGASPAAQTQIPPAQSSPKKKRKRH"
     gene            complement(24217..24495)
                     /locus_tag="P5516_02750"
     CDS             complement(24217..24495)
                     /locus_tag="P5516_02750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91390.1"
                     /translation="MYTDQDITNLVTRVALIEKELEKPPASRQSMPELGILSSNFLTR
                     AFSVWGLYFVANLLIGIVFGCIFVAIGLALGASFTDILQHILKGVPLS"
     gene            complement(24564..25961)
                     /gene="lpdA"
                     /locus_tag="P5516_02755"
     CDS             complement(24564..25961)
                     /gene="lpdA"
                     /locus_tag="P5516_02755"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRT91391.1"
                     /translation="MEEYDVIVIGAGPGGYTAAIRSAQLGKKTAIIDRRWLGGVCLNV
                     GCIPSKSLLKNADVALTLRERGREFGFSMDNLQLDFTSAVKRSRQVSDRLTRGVAYLM
                     KKNNITVYMGEARFSSRESVTLTGKDGTAQELKARDFIIATGSTPFSLLGVNIDGVKV
                     LTYQEAILQEKLPQSVVIIGAGAIGLEFATIWNAYGSKVTLVEMLPRIAPLEDEEISA
                     ELAKTFSKRGITCLTETRVLAVETTPGGVRVSTSGQNGESVIEAEQALVAIGFKPNSQ
                     NLGLEEIGVELTERKHIRVNDQLTTSVPNIWAVGDVTGKLLLAHVASAQGIRCAEALA
                     GLAVTPLDYTMIPRATYSHPQVASFGYTETQARALGKTVKIGKFPFQPNGKALGLGDY
                     GGFAKLVVDEHSGHLLGAHLIGPEVSELLPELTLAQLNGLTAGDIARNVHAHPTLSEV
                     LMEAAEMVEGKAIHI"
     gene            complement(26103..26822)
                     /locus_tag="P5516_02760"
     CDS             complement(26103..26822)
                     /locus_tag="P5516_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91392.1"
                     /translation="MRISIIIPVYNEEKTISQVLDLVLEKVRNLGEVIIIDDGSKDNT
                     GQISRDYEARNPLVHYYQQPRNMGKTEALKEGFKRSNGEIVIVQDADLEYDPAEINDV
                     VAPIQEDKADVVLGSRFLVRRASRSLYFRHYMANRLITFLSNLFTDLNLTDVETCYKA
                     FRGDIIRNMVITSTGFGFEVEVVAKVAKLKCRVYEVPISYYGRTYEEGKKINATDGLM
                     ALWYIIKYNVFTSKKSSFRKS"
     gene            complement(26915..27793)
                     /gene="folP"
                     /locus_tag="P5516_02765"
     CDS             complement(26915..27793)
                     /gene="folP"
                     /locus_tag="P5516_02765"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HRT91393.1"
                     /translation="MRQQTLPEFGKKTYVMGILNVTPDSFSGDGLLSQPDTVRAALEQ
                     AQRFIEAGADILDIGGESTRPGAEAVNEAEELARVIPVIEAIRSSGGEVLLSIDTYKA
                     PVAAAALEKGADWINDVWGLRADPLIAATAARYQAPIILMHNRSKPANAKLQQRLGGR
                     YIGIEYANLLQDICAELLESVTLARAAGVDDRRIILDPGIGFGKTVEQNLELVNRLGE
                     IKALGYPLLLGASRKSFIGYTLDLSPVERVEGTAAVCAIGIARGADIIRVHDVEVMAR
                     VARMTDALVRPRGSPS"
     gene            complement(27820..28311)
                     /gene="folK"
                     /locus_tag="P5516_02770"
     CDS             complement(27820..28311)
                     /gene="folK"
                     /locus_tag="P5516_02770"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRT91394.1"
                     /translation="MAKKETVYIALGSNLGDRAEFLYRARQLLSEFITEMRCSRLYET
                     PPWGVLEQPLFLNQVLRGDTTLPPLKLLDSLKNIEKHLGRKATLRFGPRVIDLDILLY
                     GERVIRSARLQVPHPRMLERAFVMLPLAELSPDLIIPGSGKSVNEVLSSLDTTGISLF
                     PSS"
     assembly_gap    28444..28543
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<28544..29159)
                     /locus_tag="P5516_02775"
     CDS             complement(<28544..29159)
                     /locus_tag="P5516_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HRT91395.1"
                     /translation="MEVIRVALVGFGHVGQALMALLESKQERLRAEFGFSTQVTGIAT
                     GHHGVAIDPEGIDTPQALRCAQKGQAISALSAVPAPASVPDFIASVPADVLFENSPVN
                     HQSGQPAASYLRAALERGMHAITANKGPVVHAYTELTRLAAQKQRRFLFESAVMDGGP
                     IFSLFRGALPAVELQGFQGILNSCTNLLLEMMESGRTFEEAVAYG"
     gene            complement(29188..30015)
                     /gene="murI"
                     /locus_tag="P5516_02780"
     CDS             complement(29188..30015)
                     /gene="murI"
                     /locus_tag="P5516_02780"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HRT91396.1"
                     /translation="MNISQHLVGVFDSGVGGLSVLREILTALPAQPVIYFADQAHVPY
                     GGRQLTEVQAFARGITQYLLRQGVELIVVACNTASVAALHALRQEFPAIPFVGMEPAV
                     KPAVEQSRSGKVGVLATEATFHTSMYASVVERFGQGITLFEDPCPGLVAQVEKGDLNG
                     PATRRILESALTPMMEKGVDTIVMGCTHYPFVIPLIQQIVGEEVRVIDPAPAIARQLR
                     RMLEAHNLLVEGQEVSVPRFITSGNPIQFQDALYLLLQQKHPVGRVFWRDGEVCERA"
     gene            complement(30032..30751)
                     /locus_tag="P5516_02785"
     CDS             complement(30032..30751)
                     /locus_tag="P5516_02785"
                     /inference="COORDINATES: protein motif:HMM:NF019342.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HRT91397.1"
                     /translation="MAPPLIGITVSQISRSHKRKANMLLSEYTNAVHLAGGCPVLIPN
                     EYPLDRLQVLRGVLQGILISGGGDIDPMLYQARPDPYANSIVKERDALEKALVELAVK
                     TDWPLLGICRGLQMLNTALGGTLFTDIPRQYDTSITHNQPNDVPPNKLIHPVTIEPAS
                     QLGRATACSELRVNSRHHQAVRETGIGLQVTAQASDGLIEAIEHPGQRFCLGVQWHPE
                     SLQAMEEHRAIFRAFLHAASQ"
     gene            complement(30833..31180)
                     /gene="rplS"
                     /locus_tag="P5516_02790"
     CDS             complement(30833..31180)
                     /gene="rplS"
                     /locus_tag="P5516_02790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HRT91398.1"
                     /translation="MSDILKAYDAPVNPNIPSLRTGDMVSVHVKIKEGNRERIQEFKG
                     VVLYQHNSGNDSTFTVRRVASNGIGVERTFLLRSPRIEKVVVERHSKVRRSRLYFLRD
                     RSGKSARLKQKFN"
     gene            31339..>31934
                     /locus_tag="P5516_02795"
     CDS             31339..>31934
                     /locus_tag="P5516_02795"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HRT91399.1"
                     /translation="MDDIAVDIGGTQLRAALYPPEGLEARVIQSIPTAGEKPALERLF
                     DLIASIWPENGAVRSIALGVAGALNPQSGVVYQVPNIPNWNGLNIIELTAKRFNVPVR
                     LGNDANLAALGEWKYGAGVGHHNLIYLTISTGVGGGIIVNDRLLLGENGLGAEVGHIT
                     VLPGGPLCGCGHPGHLESMSSGTGIANHVIRQIQAGRKT"
     assembly_gap    31935..32034
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <32035..33578
                     /locus_tag="P5516_02800"
     CDS             <32035..33578
                     /locus_tag="P5516_02800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRT91400.1"
                     /translation="MTARLRFSSKIGSNQPKDNWRDLVNMMRKSMVLFFVLGVFLFLL
                     TPSTAFADTLQPAAPLEQIQAQAESEVDIYFFWGEGCPHCAKAKPVLEDLAKKYPAAH
                     LYEFEIYNSQANQELFSQFGEAYGFTPRYVPTILIGKNIWEGYSDEFSQEITGVVEQC
                     LEKACENPGSALIAGKGYVPFGENAAAPTAVPTSAAPTPAATESPDTNTENNGATSKL
                     PIFGEVDFTKIGLFLSTALISFVDGFNPCSIWVLSMLLAITLHTGSRKKVLVIGLVFV
                     TVTAAIYALFIAGLFTVLKVVSFVGWIQVVVGLVALFFGLVNIKDYFFYKEGVSFTIS
                     DEKKPGLFQRMRKVMDAGDSWWGLIGGTIVLAAGVSLVEFSCTAGFPVLWTNLVAAHQ
                     VSVITFILLLLLYLVIYQLDELGIFFVAVFTLKTSKMEEKHGRILKLIGGVLMLTLAI
                     VMLVNPAVMSDIGKSLIVFVIAFGVTLLILLVHRVIFPRLGLHIGTEFKKSQDRNGKH
                     KAHRH"
     gene            33739..35019
                     /locus_tag="P5516_02805"
     CDS             33739..35019
                     /locus_tag="P5516_02805"
                     /inference="COORDINATES: protein motif:HMM:NF015882.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HRT91401.1"
                     /translation="MNPIDKESALSYHRLGGVPGKLVIHPSKPIENQHHLGLAYTPGV
                     AYPVLEIARDPELAYEYTTKGNLVAVISNGTAVLGLGNRGALASKPVMEGKAALFKKF
                     ADIDSIDIEIDSVEPETVISVCAAIGNSFGGINLEDIRSPECFSIEEQLKTMLKIPVF
                     HDDQHGTAIILGAALINALEITSKSLTEVKVVFSGAGAAALASASHLMKMGIPREHIW
                     MCDIAGLVYRGRVEEMFAEKEVFAQGDKPASLAEVIEGADVFIGLSAANIVTPEMLRR
                     MRAQPVIFTMANPDPEISYELAQQARPDALLGTGRSDYPNQVNNLLCFPFLFRGALDV
                     RATTINDEMKVAASQALAALAHEEVPAEVLKAYGLKRLEFGKDYVLPKPLDRRVCLWE
                     APAVAQAAMRSGAARLSLDLNDYQKALAERLYHP"
     gene            35035..35244
                     /locus_tag="P5516_02810"
     CDS             35035..35244
                     /locus_tag="P5516_02810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91402.1"
                     /translation="MAEKKPLGFRAIRVNAEASSLDKWRKQVIAGQPETGRAYAFISD
                     EGTYMPGGEGTAPSPLTYFVSGMAL"
     gene            35257..35541
                     /locus_tag="P5516_02815"
     CDS             35257..35541
                     /locus_tag="P5516_02815"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRT91403.1"
                     /translation="MSQVATKKKLQIRNERVKVSAYFHEQGSVLKGDAEGYCDHFEIE
                     VVVDSDAPEKEIQKLIDLARQMCFTEKALMETVKVEVAHTINGKTQPAKK"
     gene            35600..36037
                     /locus_tag="P5516_02820"
     CDS             35600..36037
                     /locus_tag="P5516_02820"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT91404.1"
                     /translation="MIQITTPKTKEDFKAYYDLRYKVLREPWGQPRGTEKDDYEPISQ
                     HFMAVDDETKEVVGCVKFSEKEPGVAWVSHLAVKTDLQRTGIGRQLLAHVEAQAREQG
                     FKKIGCQARLNTTAYFEKAGYKMHGLPTRYFGTTQVVWMEKEL"
     gene            complement(36101..36970)
                     /locus_tag="P5516_02825"
     CDS             complement(36101..36970)
                     /locus_tag="P5516_02825"
                     /inference="COORDINATES: protein motif:HMM:TIGR03716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF475 domain-containing protein"
                     /protein_id="HRT91405.1"
                     /translation="MDFSFIVIILQLIFLEGILSIDNAAVLGAMVTPLSDHEPIPWPK
                     ALAGFGHAMNKILGPQRTAALKVGLIGAYAGRGIMLVAASYVIQNPWLKLVGAAYLIK
                     LALDDLSAHGASGSDEDGDRPVKERTFWATVLAVELMDLAFSLDNVVAAVSLSKKLWV
                     VMLGVAIGIVMMRFAASLFSKVVEKAPVLKTAAYILVLNIGVELLIEDLGHIEIGDWT
                     RFGISIATILLALAYERFKFMKFFRPLLIWLSQGFALINGIITWIFAPIKGIFTLVSK
                     LFLRQRATPSVAE"
     gene            37221..38813
                     /locus_tag="P5516_02830"
     CDS             37221..38813
                     /locus_tag="P5516_02830"
                     /inference="COORDINATES: protein motif:HMM:NF013117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HRT91406.1"
                     /translation="MTLAGFPIPLPPQLSFLAKPLPVFLVNIAAWMVLAAFIYWVAMP
                     LIRRFTLALPGEVEDIVLGIIRRPLALLVLVIGIRVSIRYLQAAEDLQRLLIRLSMTV
                     IVALVGHIAGRLIRDVLVYYGESWARRTESQVDDILLPVLSLFGPLLLFTIGALIVLP
                     MWGVDVSSVLLGAGVLGLVLGLALQETLSNIFSGLSLLIEAPFKRGDLIRLVSGKVCE
                     VQRLGLRSTTLLSLDEQATIYMPNKVLAADYLVNLTKPTAEQRCSVSVSVEASNDLTL
                     VKDTLYRIAAGHPGVVASNLAKKTASILSQAEKIRAWAGGLSAEDPTRFELEKMAADN
                     ESAIERLAREGRVNDQLVLLKEEIRNMIRSIRSRETSGLSRAEVAEILNEHVNPVDNT
                     VRTLAEQCRDWSTTPDRWLNDHDYWQQRRLWDSRNEELLRHWNNLKNCITNPDERVEL
                     RLDDLSAVLIDWLDKEYKIVPGEWKDPMVEIKNVSADSAELELHYYVDNIRLEMDGRP
                     RRIRSELNHLIKHEFVQEGIWS"
     gene            38942..39190
                     /locus_tag="P5516_02835"
     CDS             38942..39190
                     /locus_tag="P5516_02835"
                     /inference="COORDINATES: protein motif:HMM:NF016141.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HRT91407.1"
                     /translation="MNILIWILVGAIAGFLADWVVKGIRMGLLGKIIVGILGGFVGGW
                     LFSLLGINLGNGILGAVISAFVGAVILLMLLLIIRRRR"
     gene            complement(39260..39895)
                     /locus_tag="P5516_02840"
     CDS             complement(39260..39895)
                     /locus_tag="P5516_02840"
                     /inference="COORDINATES: protein motif:HMM:NF024214.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRT91408.1"
                     /translation="MTDSTGRHDHKKVAAILLAAGGSTRMRQPKLLLHWHGQPLVRWT
                     ARLALFTGCEPVLVVTGANAQEVQTALAGLPLIFTRNPDWQKGQSTSVKAGVKALPAG
                     IDAALVFLGDQPHIPPRVPRELLRVFQQDDPAEAILIPTFQGKRANPVLLEKRLFEAL
                     ARLSGDAGARALFSQFPVKQVPFEEPDLAFDVDSPEDYQKLLARPAPLFKD"
     gene            complement(39885..40925)
                     /locus_tag="P5516_02845"
     CDS             complement(39885..40925)
                     /locus_tag="P5516_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HRT91409.1"
                     /translation="MREIWQVVQQWQAEKQQVALATVVQVTGSSLRPEGSKLVISSGG
                     AIHGSVTGGCVEGAVFEEAREVLKDGAPRLLSYGVTNQQAWDVGLSCGGSVQILVESL
                     DTPDWQAVLPPLARILAENQLAALLTVIHGDSVGKKMLVLPDGETHGSLGNRELDQEA
                     IGNLPENWATRLPLQITLKNGEVLFADFIVPPPRLVIIGASHIAIPLVALANTLQFHT
                     IVVDARSAFATRERFPHAHELVVGWPADVLQQLKLDAATCVVALSHDDKLDNPALLFA
                     ITSPARYIGALGSRTTNAKRLEDLRAEGAKEQDLKRIHAPVGLKIGARQPEEIALSIM
                     AEIVATAHGHDG"
     gene            complement(40965..41939)
                     /locus_tag="P5516_02850"
     CDS             complement(40965..41939)
                     /locus_tag="P5516_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRT91410.1"
                     /translation="MVVLSTAKEEGMTLTWDVFGIGTAAVDDLLLVERYPEADSKVNI
                     LESMRQGGGQTATAMVAAARQGARAAFCCRLGLDDLSRFTLSELQKEGVETSACHQDR
                     QGSPYHSIIIVDTQKHTRTIFHSGGNVSPPPELINAELISRTRVLFVDDNSGPAGIKA
                     ARIAASLGIPVIADVEPDPPPEVVELLPLVDHLIIGSKLAQTLSGESSEPAMAFALCG
                     TQRACCVVTAGEKGCWYAQRGASAVHVPGHKVDVVDTTGCGDVFHGVYAAALARGDSL
                     PDAVALANASAALKATRPGGRLGIPDLETARAFLHGSHTQTLEGLTHA"
     gene            42118..43494
                     /locus_tag="P5516_02855"
     CDS             42118..43494
                     /locus_tag="P5516_02855"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRT91411.1"
                     /translation="MKRITFILSVVMAFALLLSACAKPAPTEAPSVPEVPAATEAPVV
                     TEAPAAPEEVTLVVWDQFYRDVESEVMETLNAEFEAAHPGVKIERVVKTLDDLKVTLK
                     LALAQEDGPDVAQVNQGRSDMGAMVQADLLLPLNDYAAAYKWDERFSSSVASRNSFTP
                     DGVTFGQGNLYGVSPTAEVVGVFYNKTIMDANGWTLPTTFDEFQTLLAGIKDAGMTPI
                     SFGSLDGWNAIHEFSAVQHLLVSLDYINDFVYGVNNVTFDTPENQEAARVMQDWVDKG
                     YFSPDFAGIGYDDSNKLFKAGVGGVLTITGSWLAGELVGDTDQEFGFFLLPANPGHTR
                     LAIGGVGIPFSIRKTTTKADLAAEYLDWMTSPRAAELWANAGMLPAMPLPEGYVAEGN
                     PLFNDTLIAWDTINSENAVGHYIDWATPTFYDTIVAELQKLFGKVITPAEFTKAVEVD
                     YAAFLAGN"
     gene            43564..44463
                     /locus_tag="P5516_02860"
     CDS             43564..44463
                     /locus_tag="P5516_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HRT91412.1"
                     /translation="MKSQRRWPGESRFHGWLYLFPGLLIYLVFVFYPIIETFRTSFYK
                     WDGFSRERVFVDFQNYISLVTDAQFLKALLNNLVFIIFYSIIPILLGLLLASLLGRKP
                     LPGMTFFRTGLFLPQILSMVVVGVTWRWIFNPNFGLLNVGLRAIGLGAFTRAWLGDFQ
                     LALPSVGAVGTWVQYGFCMVLFLAGMQRIPNDQYEAAELDGANEFRQLLHITLPSLRA
                     ELGVALITTIIAALRVFDLVYVTTRGGPGDSTLVTGFLVYRSAFQQNQLGYASTVATV
                     MTLLIFGISLIILRFQSRDGEGV"
     gene            44460..45290
                     /locus_tag="P5516_02865"
     CDS             44460..45290
                     /locus_tag="P5516_02865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HRT91413.1"
                     /translation="MKRQAWQRLLPNYLILSAFLLLILLPIIGLVFSAFKTDIEVIKG
                     PLTLPSQLRFASFKEAWTTGRFNFFFRNSVIVTVVVVVISVFLSTLTGYAFGMLPVPG
                     KKVLYPLLLVGYMVPFEAVVIPLYHMMDNLKLTDTYWALILPQIGLSVSFGTLWMSSF
                     FSTAPSELVDAAAIDGCNRWQTLWRILWPLARPAVLTLVVLIFMWTWNEFLLALVMIQ
                     KETMRTLPVGLAFFQGRYSANIPLMAAGALIVAGPILLVYIVFQRYFIRGMLGGAVKG
                     "
     gene            45314..46657
                     /locus_tag="P5516_02870"
     CDS             45314..46657
                     /locus_tag="P5516_02870"
                     /inference="COORDINATES: protein motif:HMM:NF024303.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033926 - glycopeptide
                     alpha-N-acetylgalactosaminidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase 100 family protein"
                     /protein_id="HRT91414.1"
                     /translation="MNQKEKNLVNEAAKHALEILHQCVTPYGFRASADPVGYPQVWSR
                     DSSVTLLGALASEDKELINAGRASLDALSGAQSCWGMIPLNLNPESGDISTENAGSCD
                     ANLWYILGQYALYRATGDKDALARSWPRITSALTWLQYQDMNECGLLEIPEAGNWMDL
                     IAVRYNTLYDNTLFYAASLAYEEMLRDNPAELAPLPCHISADSIHERINLLFWIDRCW
                     VASHFAEHLEKLKAIRLEWFMLYYNIGTISSRPFYLPWVAFREYGDWCDSLANLLAIL
                     TGIADGHRAQHILRYMQQVGMAEPYPTKAIHPPIYPGETSWREYYRSRNLNLPDQYHN
                     GGIWPMVGGFHIAALTRCGWQAEADKLLVQLALANQQSSGAPWEFNEWMHGRTGHPMG
                     YAHQAWSAAMYLYAWKAVNGAELPLFDDLLSAKPARAVAEEVNTPYFHPGGGQVG"
     assembly_gap    46802..46901
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            47032..48168
                     /locus_tag="P5516_02875"
     CDS             47032..48168
                     /locus_tag="P5516_02875"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRT91415.1"
                     /translation="MGKGRLRIGVIGCGGIAQMMHLPFLQKNADRFEITALCDLSNKV
                     LSALARRYHLPDTGCFTDYQLMLKQNLDAVLVTSGGDHTPQVRAALLSGAHVFVEKPL
                     CYTQAQALELGRLAQEQKLRLMVGYMKRFDPAYQRARQLLAGLGELHYVQLNTLHPEE
                     EGYLYFQGLFQAEDIAPETIRHLRAQEDQAITAAVGEIPAYLRAEYTDVLLGSMVHDI
                     NVLRGLLGEPQKVLFAEHWPQGEKPGGITTTMMFSPDLRVVYTWVFLAELRDYSQEIA
                     LMSASQRLRLQFPSPYLRHFPTSLTHQYMKDGAAVEEKITVNYDEAFEQELLAFYDCV
                     VNDREPLTGADDARADIAVLQQILAGLHPVGLSGEAAGYMLGRN"
     gene            48170..48988
                     /locus_tag="P5516_02880"
     CDS             48170..48988
                     /locus_tag="P5516_02880"
                     /inference="COORDINATES: protein motif:HMM:NF013429.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HRT91416.1"
                     /translation="MRIALHGSSFVASQFGYTPPGSWEECVNALSAFYSPPVTFEARF
                     QGLVARVRELGFFRMDVWQPAELNWQWAGPEHIAAAVRALQHEGVSPTSLAGEFGETR
                     AEFESACALSAGIGAPLLSGTTALYHTDRAFVIETLKRHGLRLAIENEVELTAGEMLE
                     IIGDGGNGVIGTALDTGWYATHGYDPVQAIDELRQFIFHVHLKDVLPGDAHINCAYGK
                     GIVPIEACVKGLLKIGYDGVISIENHTLDHDPTGELVQAREWLESLIKSNTPLN"
     gene            48999..50036
                     /locus_tag="P5516_02885"
     CDS             48999..50036
                     /locus_tag="P5516_02885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HRT91417.1"
                     /translation="MSLIDEIHEQPQAIRKLLNSQGEAIATIAGQVRAHGIEYIYLAA
                     RGTSKNAGRYAQYLIGAVNRLPIALAPPSLFSIYHHPPLLKNALVIGVSQSGRSPDII
                     SVIEEARRQEVVSMAVTNSPVSPMADAAEFILDISCGPEVAVAATKTYTNSLAALALF
                     SAELAENAEMKRALENVPAWMEEMLGREDEIAQQAASFCSMNHCVVLGRGYNYATVNE
                     WSLKMKELSGVMAEPYSSADFLHGPIAMVKGDFPVLNVAPSGIAYPSLYELLGRLREE
                     RRAEIYSITDRDEVLQLSRAGQRLPQGIPEWLTPLIAIIPGQLFAYHLTCHKGLDPDN
                     PSGLSKVTETH"
     gene            complement(50081..50329)
                     /locus_tag="P5516_02890"
     CDS             complement(50081..50329)
                     /locus_tag="P5516_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91418.1"
                     /translation="MLNGQLFLKRFAWQPNAEYPDFNVNFEAYTDRDILELETLAPLT
                     ELQPGQSVSHSEEWLLFRSIPSPSSEEDVDRYILPLLS"
BASE COUNT        10974 a        13201 c        13788 g        12245 t
ORIGIN      
        1 acgttagaaa aagaccaacc cgaaggctgg tctttgaagg tcacgactcg ctttcttcag
       61 cgcttgtttc gccgagcgcc caacgggcga agcggcgcac gacgatattc tcacccagcg
      121 cagcgacatt ctggttgatc agttcctgca cggtgatgct ctcatcacgg atatacggct
      181 ggagcatgag cacgaattcg tttttgtatt tctttaacgc gccttcaacg atgcgcggca
      241 ggatggcctc aggcttgcct tcttctttgg ctttggcggt ggcgattttt gtttcgcgat
      301 cgatcactga ttcggggata tcggtttcca gcacatactc aggggaagtg gctgcaattt
      361 gcagcgcgat ttcgtgcgcc agggtacgaa acgcttcaga gcggcccacg aaatcagttt
      421 cgcagttcac ttctaccatc acacccacgc gcccatcccc gtgattgtag agttccacaa
      481 tgccttcaga ggctgttcgc gcggcgcgct tggtggcggt agccagccct ttttcgcgca
      541 gaaaatccaa agcggcgttc atgtcgccat tggattgttc cagcgctttg cggcaatcga
      601 ggatgccagc gccggtgctc tcgcgcaacg atttgatcat ttcagtggtg atttccattt
      661 tacagattcc ttcttctcca ggtggtcacc ggtcagagac cgatgttgat tgactattca
      721 ccgtccgttt tatccacgat atcttcaact ttgtccttga tctcatcgat gagatcttct
      781 gctttgtcct taagatcatc tacggtatcc tcgatcttgt ctttaatatt atcgacaaga
      841 tcttcagaga aatccccgat gtttttcaca atttctttgg cggttctggc gtgcttgcct
      901 ttggcagtgc tcttttcttc ttcagccgcg gctgcagcgt cttcatcatc gcttttcttc
      961 gctgatttgg attttacaga caccgtctta tccacttctt cttcaaggac atccagcgtt
     1021 tcctcagcga taaccgattt accaacgccg ggtttcatgt cgaggtcttc atcttccttc
     1081 gctgcatcgg cttttggccc aatttttgcc aaagtggaag cgccaagcag atcagcgtct
     1141 tccagttcaa cctcttcttc gaagcgcggg cggacgccag cggctactcg ggctgccggg
     1201 gtttcagccg ccggggcttc actgctttct tctttctcga ccattgactt gccttcgatg
     1261 acggcatcag cgatcttccc aaccaacaat ttaatggcgc ggatggcgtc gtcattggag
     1321 gggataacgt aatccacatg tcgcggatcg cagttggtgt ctaccagggc aatcaccggt
     1381 atgcctttca aattggcttc gtgcacggcg gtgtgctcgc gcatcacatc tacaataaaa
     1441 agcaggtctg gaacgcgctt catttcgcgt acaccagaaa ggcgcgcctg caggcgattg
     1501 atctcgcgct caatcaacag gccttccttt ttggtcaggc ggtgaatttc tccagaatca
     1561 cgtaaacgtt caagtttttc cagttcctgg atgcggctgt acatggtgct ccagttggtg
     1621 agcatgccgc caagccagcg ctcggtgaca taaggcatgc cgcaccgaac ggcttcttca
     1681 gtgatggttt cttgtgcctg gcgcttggtg ccaacgaaca tgataaaacc accctgggcg
     1741 actgtgtcgc gcaccaggtt ataagcggtt gtgagaagct tgactgtctg ctgcaggtca
     1801 atgatgtgga tgccgttgcg ctcggtaaaa atgtacgggc gcatcaacgg gttccactta
     1861 tttgtgcgat gcccaaaatg gacgccgctt tccaacaggg atttcatgga aatgatggaa
     1921 ctcatggggt attactcctt tgcgtttgcc tagcgccttc tttagccttt ccctcactgc
     1981 ttgcggcagc acgcaggaaa gatcggaaag cgcgtgtgat agtctgcggt tttcaccaca
     2041 gccgacgaat tataacatga aatttcgcgc gaacttcttt tttggtctct ttttgctgac
     2101 acttcttttt ggattttcaa aattatcctg gatacccggc gcttgtttac atcatcaacc
     2161 gcaattccac aaaatgtcgg cactgattcc cccgcggttt tcctgcctgt cgagcgggtg
     2221 gtctcttacg ggcagacgat gagggcggag gtgagcattc gatccttttg aatcatactg
     2281 atctggttct cacggctcca atcgcaggca gcttggaaat caacgcctgt gtctgtgtat
     2341 tctacggcaa atacaggttt tcccgcttcg ataaaaggca gcgtttcttc gatccattcg
     2401 taaaaaaacg cgctttcgat gatggcgaaa tcaaaaaaca tgagtgaatc tgctaccatg
     2461 ccaggagcgt tcttcagccc gatggcgaga ccgcgggcgt gcgcttcggc agcaagccat
     2521 tgagcgtaag ccaactggtc cgcgtaggtt attgggaagc cgctgtcctg tgtaaatact
     2581 tcgatgttat caggttccac gccgtcaaac cccttggctt tacactggtc caggcgggcg
     2641 cgcatgattg gcgccagaag gtcgatacgg cggatgtcca gccatctttc gcctggccag
     2701 ccgatatagc ttttaccgat aacctcgcca gggaactggc cggcatcggg gcgccagtct
     2761 tctcttgaac ccaccgagag ataacaaatc accttgcgac cctgcttgtg taatgcttca
     2821 accactgacg cgtcattctc aaacaggtca atatcatata catccgcttc cacacccatt
     2881 tctacaggca aagcagaaaa ctgcaattgc caggtgaggc caggtgtggg tcgccaccaa
     2941 cggacgtttg tggagagcgt ggagcttgga tacacggttg gactgatatt ggtggattgc
     3001 acgccgcaac cggagagcaa caagagcaaa atccaagttc gtgaaatcgt tttcaacata
     3061 tgagatgaat tataaaccgc cgtgagcgtt tggttctctg tggtgggaag atgggcgcgt
     3121 cacgggagag gcatacatgt ataattcaat caatctgaaa aagacaggaa acgacaatgc
     3181 atattttggt cacgaatgat gatggcgtac aggcgcccgg gttgctggcg ctggccacgg
     3241 aactcagttc gttgggtgag gtcaccatcc tcgcccctga ccgtaactgg tcagcttcag
     3301 ggcacgtgaa gacgatgcac cgaccgctgc gcgtgaaacc agtgctgctg gctgatggca
     3361 cacctgcgct cagcagcgat ggggcgccat cagattgcgt ggctttggcc ttgctgggtg
     3421 tggttgaaaa accgatcgac ctggtggtca gtggcatcaa ccccaatgcc aatgttggcc
     3481 atgatgtgac ttactcgggc accgtgacgg cggccatgga agcggcaatc ggtggcgtgc
     3541 gtggaatggc agtttcttta gacgcgccgg agcatcacaa gggggcgctg gattatgcgg
     3601 cagcggcccg tgttgccggc tttgtggtga accggttgct gcaaaacaca ctgccagtga
     3661 atgtgttatt gaacgtcaat gtcccttacc ttaaatatga agaaatcaaa gggatccgcg
     3721 tttcgcgcca gggtctgcgt atataccgcg acgcgctgat ccgccgcgaa gacccgcgcg
     3781 gtagaccata ttactggatt ggcggtgaag cacctactgg cgttccgcga gaagggacgg
     3841 attttggcga cctgcaagcc ggttatgttt ccgttacacc tctccattta gacctgactg
     3901 cccatgagca aatggcgcat atgcaatcgt gggactggag cgctgatgaa atacgctaat
     3961 atgtctgaca atgcgcattt gacataatta gcaggataga ctacattaat catagatggt
     4021 tcgtaacttc acttcatttc ggcgccatgg ttgcgcgagg agagaggcat ggacaaaaca
     4081 actgtcaagg actggatgaa gaacctggtg gtgtttgtgg acccggatac cacggttttt
     4141 gaagggctgc atattatgcg ccatcggttc gctaccagcc tgatagtcaa aaaaacagct
     4201 gaaaaccctg attacggcat tgtcacttct atcaatatct gcgataagat cattgcccag
     4261 ggcaacgatc ccaccaagac caaggtaaaa gagataatgg catcaccggt tttcactgtg
     4321 ccgtcttcca tgctgatcac tgactgcgcg gcgatgatga aggaaaaacg cattcatcat
     4381 ttgccggtcg cggatgagca tggacagatt gtcggcatta tcaccgccat ggacctgctg
     4441 tggatggcag aggccatggg aacgaacttc aaagaccgcg gacttagctg agcatcttga
     4501 caaaaaaaca tcccggcgca tcggttataa ttagccgtta atgcgccggt tttttatctt
     4561 gttcattcgc gttgctttat tgggcgggct gctgttatcc ctgccccagg gaactgcgca
     4621 ggctgccacg gtatcaccgg gggaacttat caacctggtg aatggtctgc gcgccgccaa
     4681 cgggctcccc gcacttattg aagatgccat cctggatggt acagcatacg ccacggcgca
     4741 ggcgatggca gccaacggtg tttgcgcgca tatcggcggt gcgagagagc gcattcgcgc
     4801 ggctgggttt ggcgggggcg ctgtcgtgtt cgctactgaa aatatggcct gcgctgtgag
     4861 cgcgacgatt tccgatatcc aatcttggtg gtcagacagc ctgcatatga tgccaatgaa
     4921 tgaagcgcgc tatacccatg tgggagcagg ggcttatacc gcttcgaatg gcaccaccta
     4981 ttacgtgttg cacgcggcgt atgtgggtga tgggggaagc gcatccttac ctggttctac
     5041 aggcggcagc gctgggcagg gacaaaaccc tgccgaacca ctggttgaac cggtggtcac
     5101 ctcgactccc caggcagacg gcacgattgt gcacactgtg cagtacgggc aggcgttgga
     5161 tactatcgcc acctggtatg gcgtcaccat cgcggaaatc aaagcgttga accacatgac
     5221 ttccaatacc atttacgtcg gcgacaacct cattatcaaa atagcgccca ctgccaccat
     5281 cacccccacg cgaactttaa cgccccggca accgacgcgt actccttcca tgacgcccac
     5341 cctcacaaaa actgttatgg tgagcacacc cacgcccacc agggcacctg gtttgctgga
     5401 tactttgccc aaaattgacc gccagtggct gggttttggt ttattgctgg tgagcgcagc
     5461 ggggctattg ctggtgcttt acttcctgtt catccgcccc aggtttaaaa aataactctc
     5521 accttctgat ccggtcattt tcaccccaca gcttcctgtt tctttgtgaa ccaacagatt
     5581 cttttctgct ggaaagggac gttccgccct gctcaccagg ggatggatcg gtctgagcct
     5641 cttacgcgaa taccgacgga cgcgttaaag aaaaacgcgc tgctcgaagc cctttgtttt
     5701 ccgcgctgat tgcgtgccaa catttcatca ggttggtgtc agcatccctg ctccatgttt
     5761 gcctttggtc gtgcaaccct gcgaattcat ttgcgtatat gggataaatg ggtattaatg
     5821 actttcactg gaggtagtga tggaaaaaag aaacagggtg acactggtat tgggcttgtt
     5881 gctcgtattg gcaggcacct attttgtact gatcaatgcc gtgccgggtt tggcacaact
     5941 gattactgtc cacttctcct ggccggtgat catcatgctt gtcggcgccg ggctgctcat
     6001 tttggggctg cttagcggag tcgcggaaat gtccatacca gcgttcatcg tcgccggcat
     6061 tggtggaatc ctgtattacc agaacctcag cggcaactgg agcagttggt cgtatttgtg
     6121 gacattaatc ccgggttttt cagggctggg agtgctggtt gctgcattgt ttggcgcgcg
     6181 taaaaaaggc gcggctcgcg acgggttgga tgccatcggt accagcctgg tactgctggt
     6241 gatttttggc gccatcttcg gggcattcaa accacttggg ccatattggc cagtgcttct
     6301 catcgcggct ggcctggtga ttggcgcacg cgcgttcatc aaagggtgca aagaagaaac
     6361 acctgtaaac gtcatcgatg taaagccatc aggcgaagca gatgaaaaca tgtaggaggt
     6421 aacatgtctc agcaatccag gcgaatctta ctgggggtgt tgttggttgt ggctggcgcg
     6481 gcctttttgc tgcaacagct gctggaaatc cccatcggcg gactgtttat cgctttcctg
     6541 ttcgtgctgg cgggttgtgt ttttctgtat ttcctgctgc gtgatcgcca taagtggtgg
     6601 ctggccatcc cggggttcac cctcattggc attggtggtg ttatcttgat aaaaaagctc
     6661 ctgccgaacc tggcgggcga ttatggcggc gctttctttc tcttaatggt ggcgttgggc
     6721 tttctggtgg tctttttgct caaccaggcc caatggtggc cggtcatccc cgcgggaatt
     6781 ctgaccacat tggcgctggt ctccgcgctg gacttgaatg ggatggccaa cggcgggctg
     6841 ttgttcctgg ggataggcgc gactttcacc gtgctcgggc tgatgccggt ggggaaggct
     6901 gaaaaatggc cctggatccc ggcgggcatc tgcctgctgt tgggattgat cttgttcgcc
     6961 agttctggcg cgctgatggg ttcggccgtg ggctgggtgt tcgctttggt tctcatcgcg
     7021 gcgggggcgt acttcgtcat tcgctcgctg atcaaaaaag cataggcagg tgaacatgca
     7081 aaacaggctt ttacgttcaa agcaagatcg tatcctgggt ggcgtatgcg cgggcttggc
     7141 caggtacctt aacattgata ctgtgctggt acggctgttc ttcattgtct ttaccctggt
     7201 gggcggcatt ggcccgttgc tgtatatcat cctgtggatc gtcgtccctg aagaccaggc
     7261 cgaaagctcg tcgggctact cgttcaatgg agaggttctt cgtgaacgcg cggagaactt
     7321 tgcagacgaa tttgtaaacg cggttcagaa acctggcaac aaagccagtt tttacgtagg
     7381 gattgggctg gtgctgctcg gggtttacat ttttctcaaa aaccttaacc tcccctggct
     7441 ggcatggttg aacaccagtg tcatcctcgc tttgctgatt atcatcgccg gcgcggcgtt
     7501 gctttacgtg gcgttgaaaa gaaaaacata gtgccaattt tgggcagttg ttggcttgca
     7561 caggaacttt tcgcaattca gttcgtctta ttggtaaaaa caagaatgag aggaaaaaat
     7621 gaaaaaatca ctgttggtta tcgttgcaag tattatggcg gtcatgtcat tagcagcctg
     7681 cgcaggcagc gggcagccgg ctggcatgcc gaatctccgc accctaagcg cgagtggctc
     7741 aggggaggtt tacctggttc ctgatgttgc ttatatcaat gtgggtatcc gcgttgatgc
     7801 tgatgaggtt tccgacgcgc tttccaagaa caacgcccag gcaaacagca tctctgaggt
     7861 gttacagcag ttgggcgtgg ataagaaaga cgttcagacc tccaacttta atgtttaccc
     7921 catgatggat tatggtatcg atgggcaggt ttcacgccgt tattacgtgg tcgaaaacac
     7981 ggtctatatc accgttcgcg acctgacgtc attaggtaaa ttgctggacg cggtagttcg
     8041 ctcgggcgcc aataccatca acggcattag ctttgatgtg ctcgataaga ccgcagccga
     8101 agccaaagcc cgcgacatgg cgattgccaa tgccaaagca gaggctgaag ccattgctgc
     8161 gtctgccggg gtgaagctgg gtaacctgca atcggtgaac gtctataccg gcggaatcgc
     8221 cgtgcccatg tatgacgcga aaggcattgg cggcgcggcc atggattccg tggttcccat
     8281 ctccgccggg cagctaaaga taaacgtaga ggcgagcgtc atttacgaaa ttaaataaag
     8341 cgtaatggca gtacaaagag agggtgtctc cggtggcacc ctctctttac gtcttcaagg
     8401 cttatttgta tttaatcgaa ctgatgcgca ttaatttgct caacttatgg gttgagataa
     8461 cgcgccttac cgtgcctgaa aggccacgca tcaccacgct gtcagttcgg gcgccatctt
     8521 taaagtaggt taccccacga aggtaatctc ctgtggtgat accggtggcg gcaaaaaaga
     8581 cattatcgga agagatcaag tcgtccatgg tgagtggttt ttcgaaatcc acgcccatct
     8641 ctttggccag gtcaagttca cgttgattgc gggggtaaag cttgccgatg atctctcccc
     8701 ccatgcagcg caaaccggcg gctgccagaa cgccttcggg cgttccgccc acgcccatca
     8761 atagatcagc ggtattgtct cccatggagg tcatcaatgc accggctaca tcgccatcag
     8821 ggatcaactt gatacgagcg ccagccatac ggatctgcgt gatcagttcc tggtgccgcg
     8881 ggcggtcaag caccactaca gtcaggtcct ccactttgac gcctttggct ttggcgacct
     8941 tgtgcaggtt atcgctcact ggtgcgtcaa tatccacggc gtgccgggct accgggccaa
     9001 cggcgatctt gttcatgtac acaaaaggcc ctgggttgaa catggttcca cgcggagcca
     9061 gcgccacggt ggagatggaa ttccataaac cgtaagccag cgggcgcgtg ccttcaatgg
     9121 gatcaaccgc gatatcgact tccggccctt cgccggtgcc cagtttttct ccgttgtaaa
     9181 gcatcggcgc ttcatctttt tcaccttcgc cgatgataat ggttccccgc atgctgatgg
     9241 agttcagcat cacccgcatg gcatccaccg ctgctttatc cgcgccgttc ttatctcccc
     9301 tgcccatcat cctgcccgca gcaagcgctg ccgcttcagt aacccgaacc aggtcaaacg
     9361 ccaggtttcg ggtgggtgta gtatttggca tgatccctcc gtttaaaaaa tgatgttggt
     9421 gttatcgtgt cagtaagatg atcacgtaat acccgatggc tgcagcccaa agcgaagaat
     9481 ccatacggtc gagaaacccc ccatgctcca gcaggaaatt ccctgaatcc ttcgcgttga
     9541 actgacgctt aagcatactt tcgccaaaat caccgaaagg cgccaggatg gagatgaccg
     9601 ctccgagcac cagcccatac catggtagaa tgagcggggc cggtttgtgc cacaatgcag
     9661 ccagtgccca tcctcccagc atgcccacca gtatgccgcc caggtaaccc tcccaggttt
     9721 tcttggggct cacacggggg agcattttat gtttgccaag aaggcgtccg aaaatgtatg
     9781 cccctgtatc tgccagggat atgatgggga agaccagcac cacccaatgg agcccatttt
     9841 ccagggcgcg cagtgaaatg gcataactcc ccagccagcc caggtaaacc gttccaccaa
     9901 tcgtgatcat caagtcgatg gcggctgtca tccctccgcg attctgctgc agcacgtgaa
     9961 acaacattga ggtcagcaca agcgcagcca gccagacgtc actgtagctg aacgcgtaaa
    10021 gatgtcgcag aacaaccgcc agcggcacga aaatccccat caccaacagg gatggggagt
    10081 attgcccttt gttaaacagg cgccagagct cccacgctgc aagcaagagc accaccgcga
    10141 caaaaacggt aaacgcccac ccgcccagga tcgaaaagac gatcagcgtt ggaaccatga
    10201 gattgataac gatgagccgt tttcgcagtg agagatcagt tttcattgtt tgaacttgaa
    10261 atgttcccga accttctgtc gcgttgatcg taaatttcga tcgctttgaa cagttcttct
    10321 ttagtgaaat ctggccataa agtgggggtt acataccatt ccgcgtacgc gccctgccag
    10381 atgaggaagt tgctcacccg catctctccc gaggtacgga tgatgagatc cgggtcgggt
    10441 gtgccagcgg tgaacaggta gcgatcgaca agttcctcag tgacatcttt tgaggggatc
    10501 ccgtccctga tcattttctg aatggcgtgc acaatttcat ccctgccgcc atagttccac
    10561 gccaggtgta atatcagcct ggtgttgctg caggtaacct ccatcgaatg ctccactttt
    10621 ttgcgcaccg actcgggcat ggcttccagg cggccaatat gatgcagtcg cacgccttcc
    10681 gcgcacagct catccacttc tttatccagg taggtttcca ggatggttaa caaaccgtcg
    10741 acttcggttt tcggtctgct ccagttttca gttgagaagg cgtacagggt caggtgctgc
    10801 acgccaattt cagcgcagcc cttgataatc tggcgcaggt tttctgttcc ggcgcgatga
    10861 ccttccaatc ggggtaaccc gtgggctttg gcccaacggc cgttcccgtc cataatgatg
    10921 gcaatatgcg cggggatctt tgccggagtt ttctttgtca ttcgggcgcc tgaccaggtc
    10981 agatttccat gatctcagat tctttgcgct taccgatgag ttcaatttca tcgatcatca
    11041 ggtctgtcag tttctgcagg tcctgttcac cggtttccag atcatccttt gaaatcattt
    11101 tctctttttc aaaatcgcgc aggtctttga tcaggtcacg gcggacattc ctgatggcaa
    11161 cccggcattc ttccagccgg ttgttcacca ccttaaccag ttcgcgtctg cgttcttcgg
    11221 tgagcggtgg caggttgagg cgaatggact tcccgtcatt attgggggtc aaccccagtt
    11281 cagatgccag aatagaacgt tcgattgttt tgatggttga aggatcgaaa ggtttgatta
    11341 agagcgcgcg ggcttcaggc acggatatgc tggccaactg cagaagcggc gtgggcgcgc
    11401 cataataatc gataggtagc ttttccacca gcgccggcgt ggctctgcca gtgcgaattg
    11461 ccgtcaggtc ttcttccaga gaaagcaagg cgcttttcat gcggttttca gcgtcttttg
    11521 tgatttcctt taacatattt cctccgccaa ttgctgattt gtaataaagc ataccttaaa
    11581 agcgataaaa aatccattga catcaagcaa tatcttacag taaaacgcat ccggcggcag
    11641 gtattgacaa ataagaacta atgttctata atagtgataa gtccggcagg ttattctggc
    11701 ccgaaccttg caataagtca aacaactgaa aacttatgga gagtgagatg aatactggca
    11761 tgctatggtt cgataatgac ccaaaggctg atttcttaac caaaatcaac cgggcaaccg
    11821 attattacct gaagaagtat ggccaacgac ctgatttatg ttttgttaac cctggaatga
    11881 agttcgaacc tcctccaaaa aatgtaggga ttgaagtgca aaccgaccaa atgattatgc
    11941 ctgaccattt ttggctgggc attaaaacct gttcagcctg atgaatctgg tacaacttgg
    12001 tgttttagcc gcccaactgg cggctttatt ttttctcctg gttgaaaatt ctttttttta
    12061 accgctgcca aagttgagaa acgtgcctga aagggcgctg tgaaaaccgg ttggtgaaat
    12121 cagtggctgc tgattccagg ctgatgcgcc gtttctgttc ttcagccagg taccagcggt
    12181 gttcaatgat ccaaaaatat aggtccatct ctgtgcgggc aggaaaagcg ttgagaatct
    12241 gatgtttacg gatgatccgc accagtggaa ggtaaacctc gcggtgccat cctttcaccg
    12301 cctcttcatc tgtaactggg cgcgttaatt tctcacccat gaaccagcgg tgcacgctga
    12361 tgtgctgcaa taccttctcg tactgtccag gtatggaaaa acggaagtct tcacctggat
    12421 aaaattcgtt aaggcgggtt tgcctgagga actcgctata ctcctgctcc agtaccagtg
    12481 cgttcacatc ggtatcacgg gtgattttgc cgctggtgtg cacttctgtc accaaagcgt
    12541 ctacgtaaac ctgtcccttt tcacgggcga cggatacgcg gtgattacca tctttgacaa
    12601 aatagaaatc acccacttta tacacttcga ttggcggcag gatgatctgt ttgaaatgcg
    12661 cgcggtcgat actttcccag cgcgagcgcg tatgcgtctg cctgggcagg aatgcacggt
    12721 caaaatcatt gtagcggctc acggaaccga ttatcttttc agtttcgatt tgcctgaccc
    12781 caatgtagtt ttgtccgcgc atcgggatat gcttgaggac ttcttcaaat ggcagcagct
    12841 cattgttttt cttctgaatc aggtgaaaaa cggagcgcca aaaacctttt tgataggcgt
    12901 ggctaaaatc gtgacgcgat tgaagtatta gacgacctga atcaatgtct tcttctgtca
    12961 tcatgattca cctttgaccc cgccaagagc gcgggcgcgg ggcgggaatg tgaagggtct
    13021 tatagccgta aacattctga accgtggtgc cgccgatcag cgtacgggtg atggtttcac
    13081 ggcggtaaat atggatgtgt ccatgcagaa agtaagctgg ttgaaacact tttatcaacc
    13141 agcgaaaggc tttaatccct cggtgcggca ggtcctcttc atcgtgcacc tgccatggcg
    13201 gcgcgtgagc catgaaaata tccaggtagc gacccagcgc cagtttattc cagaagaacc
    13261 ttggcaccaa actcagcacc atctgccagt actgcccctg ggtatactgg tatggaccct
    13321 ggttgtatct taaagaccct tcaagccccg ccagcagcag cccggtgtca tcctccacgc
    13381 atcgatgatg cacgtccact gctccccatg gatatttccg cgttcctgcc acgccgtact
    13441 cgcgggcgtt ggcatgatta ccgcggacaa aatagcaggg gacatttaac atgctgacca
    13501 tatattcgat atagtaatag ggcaggtcgc cgcaggagag gataaggtcc acgtcttgga
    13561 acctgtccat gatcatgggg ctgtaaagca aagcattctc tacatcactg actgccagta
    13621 tcttcatctc aacaccgccg gcttatctct tctcaaccag ggcggtgacg caggcagcag
    13681 cgaggttgcg ggtgtgactg atggaaaccg accaagagcg ccagttcata cgctcagcca
    13741 tttgagcagc agcaccatac agccttaata cgggcttccc attttcatcc aattgggttt
    13801 caatatcctg ccagccaact tcgccaatac cgcaacccag ggctttaacg gtggcttcct
    13861 tgatggcgaa ccgggcggcg aggcgatcat cacgccctcc gctatcttct atctcctgtg
    13921 ttgtaaacac cctggagaca aaccgggctt taatcgccgg ggtgatcgag cgcaggcgct
    13981 caacctccac cagatcgata ccacaagcca cttccatcat gggttattgt tgacctttcc
    14041 atccgcgcct gctgaaacga tgatcaactt atcagggtta atgaatttcc gcgccgcggc
    14101 gagaatatgt tctctggtga gcgcgcttac aagggtgggg taacgttgca ggtagtccag
    14161 accgagttca aaacgctcaa gatttaaaat actgttagcg accccggcgt tcgattcgag
    14221 ggaaagcggc atgcggccga tatagttatt ctgattgtct tcaagctctt cctgagagac
    14281 aggctcagtt gtgaaccggc gcaattcttc aatgatgagc gcggtggctt tttccacgtt
    14341 gccagggctt acgcccgcag aaacttccca cgaaccgctt tcaatccagg cgttcagact
    14401 ggcggaagcg tagtaagcca ggccggcttt ttcgcgtact gcatcaccaa cacggcccat
    14461 caaaccaaac tgaccaagga tactgttgcc gagcgacgcc gcgaggtact cgggatcttt
    14521 gcgcttgggg ccgagcgttc cgattatcag atcggtttga ctcttgcccg gaatgatgac
    14581 tttgcgcgtg atggatgctt gcgggggttt tatcgcctta aaggagcatg tttgccgttt
    14641 gtgaccggtg agccaattgc cgaaatgttt ttcagccagt tcaaacaccc ggtctgcgct
    14701 caccgcgccc accaccgcca gtttcatccc ctcaggtcca tattgctggc ggtgaaagtc
    14761 agccaggtca tcccgcgtga tattttggat ggtctccgtg tagccatcct caggtctgcc
    14821 gtaaggatga tcgggaaaga gcagcgaatc aaactccagc gccgccgtat cgcgcgtgct
    14881 ttgcccgcgg atggccaacc cggtgagcaa ctgggcgcgc aggcggctca cctgttcgga
    14941 tgggaagaca ggtgaggaca ggcattctgc gagagtggcg aacaataatg gcaggtcttc
    15001 agccagcgcg cgaccgctga aactggtggt gtgaacactg gcgccaaacc cgagagaagc
    15061 gcctgcggat tccaaccggt catacaggct ctggaaatca ctgctctcgg tgccgcgcat
    15121 aagagcgagg gcggtgaagt gggccaggcc gagtttctct aacggatcca gctggctgcc
    15181 agccgacaca tatccgctta tgaccactga cgaactgttg aagttgctgc gcgttaaaag
    15241 cacgattccg ttgcgcagta cctggcgagt aatatccgct ggtccaggga tggaagacga
    15301 aagggtgtat ttgggtatca tcgtttcgct ccatccggac ggtaaatccc taccacgcgc
    15361 tggttttgat tcaggtattt gcgcgctgta tccagcagga tctccggggt cacttctgcc
    15421 aacctcgagg tatagcgggt gaaccaggag tattgatcaa acatctctga gtaacccagc
    15481 cagaatgcct ggttggtaat attctcactg ccgtatgaga aaagtgcgcg agcctgtttg
    15541 gctgcgcggt cgatgtctgt ttgtttcacc ggggcctggc gcagaacgtc gagctcttta
    15601 tccagctgct ctaaaacgac ttccggctgg tgctttgggt ggacggtgat ggtgatggtg
    15661 tatagaaacg gatcgtgggt tgcctgcagg gagccgttaa ggctgatagc gtattccttt
    15721 tccaccagac gccggtacag gcgagaagtc ttattcgaaa tcccgccccc accgaacata
    15781 ttcaggctgg tgggcccggt gagcagacta tcgataatgg tgaaggcaaa aaactcagct
    15841 tcagttgccg ctggcgaacg ccaggcgacc tgcaggtagg tggtttcgcc aggcccggcc
    15901 acctcgatgc gtttttcttc cgccaataat tgttcccgca agtattccgg ttcaggtacg
    15961 gagtgaccgt gaagcggctg gtaggtctgg cggatccttt ccaatatttt ttcactgtca
    16021 aagtcacccg ccaaagcaag aaccgcgttg ccggggttgt aatacttgcg atagtgggcg
    16081 tacaggtcat cacgggtgat actccgcagg tcggcgatcg aaccgatgac ctcattctta
    16141 tacgggtgat ggtcaaaagc ggcgaattgc acggcttcat ccagtagaaa gagaggttca
    16201 ttctcgttac cttcacgctc tgagataacc accgtgcgct ccgagttcac ttcgttttca
    16261 tcaaataaac tgttcaccat gcggtctgct tcaagggtca tggcgagatc gatcttgttg
    16321 gccggggtgg tttcaaaaaa ggtggtccaa tcaaggtagg tgaaagcatt ccaatatcca
    16381 ccatcccgtg aaatggcttt gtccagtacc ccggcaggga attttgctgt gcctttgaac
    16441 tgcatgtgct ccacccaatg ggatataccc gtgaagccgg gtttttcaaa gcgcgaaccc
    16501 acgcggtacc aaacccactg gctgatgatt ggcgcggtgt gaatctcctt catcaatact
    16561 ttgagtccgt tttccagttc cacctgcgtg attgtttccg tcatacgacc tctaaccctg
    16621 ttgttgcagt aactatagca caagcacgaa acgcttggta gggaagttgc tataataaaa
    16681 gtgtgattga tttcctgttc attaatcaaa aataaaccat aatcataaag gagtcttcat
    16741 gtccaaacga atccaggtgg ccataattgg cgcaggacca gccgggttat ttgcagccga
    16801 gaaactgacc caggcagaca tcgcggtcgc tcttttcaac cgggatatca aacccggcgg
    16861 tatggcggag tacggcatct acccggaaaa gcatcaactg aaggatgggc tgcgcaagca
    16921 gttcgaacgg atcctttcgt atgagcaggt gcattatttt gggaatacct gtgttgggga
    16981 ggaccaaagc ctgacgattc cccgcttatt ggagtggggt ttttccgcgg tgttgatctg
    17041 ctgcggcgcg cagggcacta aatggctggg cattccaggt gagaacctcg aaggggtgat
    17101 tcactccaaa aacctggttt tccattacaa ccggctgccg ccttactgca ctgcgcccat
    17161 caaaattggc aaacaggctg tggtggtcgg cgcgggcaac gtaatggcag atgtcacgcg
    17221 ctacctcctg ggtcttccac agatggaaaa gattcacgtt tgcgtccgcc ggggtccggc
    17281 ggaggttaaa ttcactgcca aagagctgga atccatcatt ggcggaatgg acatggatgc
    17341 gctggagcac gagtttgaac gggttaaacc ggtgatggtg ccacttgggc aagaccctga
    17401 agttgaaaaa gcggtctatc tcaaggcgtt tggaaaggcg ggaaagacag gcttaaaacc
    17461 cttgatcgaa atgcattttt tggtttcacc cgtgaagatt gagggagatg agcagggcgg
    17521 ggtgaatacg gttgtattgg aggaaaacac cctggttttg agcggagaaa aaacctcggc
    17581 ccgcgggctg ggcacacacc gatctctcgc agccgataca gtggttttcg ccattggcga
    17641 cagggttgaa gaacgcctgg gcgtgccggt gtcgggcaat gagtaccgcc atgtggagaa
    17701 cccgcgctac ccggtggaag ggcagtccta cgaggttgaa gcgcccggca gcgggcaggc
    17761 gtggaaaggg gtgtttgtcg ccggttggtc gcgattggcg agcagcgggt tggtggggaa
    17821 cgccaagaaa gatggggtga atggagcgga agcggtcgag cagtacctgc gcgaagaacg
    17881 cccaggcggc ggcctcttgt tggacgaact ggaaagccgg ctttccagtt tacctgaccc
    17941 agttgtgcag caacgacacc tggcgcgact gctggctgaa gaacgtaaac gcgcgggtgg
    18001 tggaccgatc gaatacgcga agtttacgac caacgctgaa atgctgcagg taatggggtt
    18061 gcagtgagcg aagatagcta ttgaggtttg cttgcgggag gaaagctcag gataaagccg
    18121 atggcgataa tggcgcagcc gatttccata cccctggcct gccacacgcc aaagtaaggc
    18181 acgcctggca atggatgtgt gcccacccca aacacatcgg ccaggccaga aaaagtaaca
    18241 atcacgtaac cggtgctcac caggcgcata ccgatatctg cgctgatgct gggaggttga
    18301 tggcgccaca acgcgcgcac cgaggtgtac ccgccaagac aaatgatcat gatgccaata
    18361 agcatcaccg cgatttggat aaaaccaacc accgggctgc ggtccagccc aaagatggca
    18421 ggtctggctc ctaccagaaa gatgataaaa ccaaccagca tcaccagcag ggcgaaatgg
    18481 atgcgcgcta gcggcagcgc gggtaaactg ttggcagtta ctttgggctt gccctcattc
    18541 tcaatggcgg aattttctgt catccgtata ttctaactcc aagatggcct ttcccgatat
    18601 tgctgtctcc caagtataac ccgaaataaa agaaggcggt tacccacgaa aatgacaacg
    18661 cgcccagagg gtcgggcgcg ttgattcatg tcaatattct ttaggcttca ggggctggtt
    18721 ctgtgggttc ttctgcggtg gattgatcat catccttggc gattaccgct gtatcttcgt
    18781 cgagaatgcc ctcgagagcc tgccagtcca tgtctgcgta agccatcgaa tccacgcggc
    18841 gcaggctcaa accgatccga tgcacgctgg gatcgatctt gatgatgcgc agggtgcaaa
    18901 tgtcgccttc cttgaggact tccttggggt gttcaatgcg ccgttcgctg atctcggaga
    18961 tatggatcag tccttcgatg tcttccgcta acctggcgaa cgcgccgaat tttgtcaggc
    19021 gggtgatggt gccttcaatc agttgaccca cctggaattg cgcagccttc tggtcccagg
    19081 ggtcagattg cagctgacgg atggacagcc cgatacgctt cttctcgtgg tcgatattga
    19141 tcacttttac ttttacttcc tggccgactt tgagaatttc gctgggatgt tgaattcgct
    19201 cccaggagat ttctgacata tggaccagcc cgtcggcgcc gttcacgttg acaaagacgc
    19261 caaagtccgc caggctggtg atacgaccca ggtaaacttc gccttccttg agctcttcca
    19321 gcacgcgctc cttgaccgat tcgcgggttt cagtgctggc ctgtcgttca gaaaggataa
    19381 ggcggcggcg ttcgcggtct acttcgatga cgcaaacatc aatttcctgc ccaatcattt
    19441 tcccccaacg ctgttcaggg gtatcgccgg tgacgccggc gcggcgcgtc aggctaatct
    19501 gagaggcagg cacaaagccg cgcagcccgc cgataggcac gatcaacccg cctttgttgt
    19561 aaccgaggat ggtgctgtga gtggcctgtt tgctcgcttg cagggcttca acttcctgcc
    19621 agctttcttc ttcacgggcg cgcatgtagg agagaaccag gttgccgttt tggtcttcag
    19681 gattgacaac ataaacagaa atctcatcgc caacttttag atttgccagt tcgtcgcttg
    19741 ggatcagttc gtattccttg ccggtgatta tcccttctga ttttgtcccc acactcacca
    19801 gtatctgccc tggagtgatg cttgcgatta tccctttgcg gatttcaccc tgggtgggaa
    19861 aatcaatgcc taagccttct ttctcaagca gagaagccat gttgttgtcg ttctggttat
    19921 tgtctaaatt tgctcctgat ccattgtgcg tgacattctg atccatatga tttttgaact
    19981 ccggcgttta agattagcca taatgagttc aatcgaatga ttgataaccc aaggctacaa
    20041 taatacatca ttatacatca tttgaatcga tattttttat cgattcaaat ggtctttcag
    20101 ccatgtcttt gtaaatcctc ttaatgacgt tttcgtttct ttttcggcga ggactgcgct
    20161 ggcggtattt gtgtttgcgc tgctgggctg gcgccttcag gggctgatct ttcagcaggc
    20221 tgtccgccgg ggcggcgcgg ttgaaacagg aacatacggc tgatcacacc gccgcgaatg
    20281 tcgctgagca ggtttttgaa cagttcagaa gcctgaccct tgtactgtac cagcggatca
    20341 cgctgcgcgt aggcttccaa tcccacggag actctcaacg cgtcaacccg ggtcagataa
    20401 tcaacccaca attcagagat cacactcaat aagatatgcc tgtagatttg attttgcaca
    20461 cgccgaccga gcgttttttc gaccgcggtg cgcgcttctg ccgagaggtc atctggctgg
    20521 gcggtttgta aactgagaaa agcagcatct ccaacttctg cgcgcagaga ctgttgcaac
    20581 cccgcgtcaa gctggctcat ggagctgccg gacaggcgga aacgctcaaa ttcaatttcc
    20641 ccccaaaccg tttccaaacg ttcgatggtg ttttcaaggt gctgcaggat ttcactggtg
    20701 atctcagtca ccgggcggtc ttggatcagc ctggcggcgt agaagatata attcaccagg
    20761 atcacgcgcc gcattccgcg ctgatgggtg cggggatcta tggccacctt agagccgatg
    20821 cttgtgttca gcagcaggtc cacgagacgg ttctcatcac tggaaccgcg cggcgacttg
    20881 ctcagagcgc cttccaggtt ctggataacc tgaccctgcg ctgaaagcag tgaagcagtg
    20941 cgcttggtca gcaaattctc tacctgttcc atcagcgtat ctgaaatact tgtccaatcc
    21001 tggtcctgca ggtcagcggc tttgaggttc aggctctcgc ccagccggcg ctcaaccgcg
    21061 ccaagcgttc gcataaccgc taattggtta agcgcgcgct tgacctggtt attgaccttt
    21121 tcttttactt cgtcgtctcc ctctggcagc gccctcatct catctgttgt caggcgcagc
    21181 tgggtgcggg tgagattgac caactcttcc agtatctctt gcggtcggcg gggcgcttct
    21241 tcttctccgt ggctgctacc gagcccatcg aaaatggcat ccacgctttc cagccgctcg
    21301 gcggtgagct gatcgatggt ttcttccgtt ttttgaagca acagactggt ggcgttcagg
    21361 atatgctcgt tttcagcctg cagcgctttt tcagtgattt ctatgatctg gtctttaatg
    21421 ttgctgtcat cggtttgacc gttgatacgc cgttgcagtt catcaacaat caggcgaatg
    21481 gtgtaggaag gcgtgcggat accttctgct tcaaaaacca tggaaggctg tacttcttcc
    21541 aggtaactca ataacttcca tggaccttct tcatctttca gcgccgctgg gatgcgttcg
    21601 ttgatttctg tgcgcagaat ttcaagcacg tcttcatgca ggctctcttt ttcgaaggcg
    21661 cggttgcgtt cggcgtaaat gcgcttgcgc tgcgaattga gcacatcatc gtattccaac
    21721 aggtgctttc gcacatcaaa gttgttgcct tccacacgct cctgcgcctg ttccaccatg
    21781 cggccgagca aaccgctctc aatgggcagg cttggatcga ccttcatgcg ggtcatcaac
    21841 ccttccattt gcgcgccacc gaacaggcgc atcaactcat cctccaggga taggtaaaaa
    21901 cgagaagatc ccgggtctcc ctggcgggcg gcgcggccgc gcagctgatt atcgatgcgc
    21961 cgcgcctcgt gcctttctga gccgatgacg tgcaaaccac ccagggcgcg cacgcgcttc
    22021 atatcctcaa ggtaacgaat aaaggtattt accgactcct cgtagatgcc gtaatcctcg
    22081 agcgtaagtt tttccagttc accgcggcgg gtatcgttgt tcaaatcgta gggatctgcc
    22141 acaccggcct tctccagcac gcggatcacg tcggccagca cttcctcgtt cagttcaccg
    22201 ccgagcttga tatccacccc acggcctgcc atattggtgg cgatggtcac cgagccaaaa
    22261 gcgccggctt tggcgatgat ctgagattct tcatcatgtt tgcgcgcgtt caatacctgg
    22321 tgcggtacgc cgccttttaa tacttcaagc acgcgctctt tgtggggtgc ctccaggtcc
    22381 attaccttga gcaggcgttc caatagatca gggtcttccg gattgatcga agaaagcccc
    22441 gcctgtcggg cgagcgggcg catttctgcc acattgagct catgcaaggg gcggttgaga
    22501 aattgcagtt cggtgatctc gatttcggct gatttattga acttttccag ccaggcatcg
    22561 cggatgacca gggtttgtgc caggcggcgc accgattcag cgctgagccg ctgtgaaagc
    22621 cgttcagaat gttccacgga ggtcgtgccc accagttgcg gccgaccaat ggcgtaataa
    22681 tggatgattt ctgcgacaat cgctcgcagt ttaccctctt cggtgcggta aaccaggtca
    22741 ggaaagtcct gacgacgcca aaaaaccgct tcgtgatgag ggtcatcctt cttgctgtaa
    22801 tactgcaggg ggtaattttg ccggtcgcgg gtctccacca cggtcagcgg gctgtcttgc
    22861 tgggcggcca cgtattccag gttcatggga atgggcagca cttccagctt atagatcttc
    22921 gaaaattcct cggcttcggt cagggcggta ccggtcatgc cggccagttt ttcgtacatg
    22981 cggaagtagt tctgaatggt gatggtggcg taggtcacat tttcaggttc aaccttgacg
    23041 ccttctttcg cctccacagc ctggtgcaac ccttcagacc agcgcctgcc gggcataagc
    23101 ctgccggtga attcgtccac gatgataacc ttgccgccct ggacgatata atccttgttg
    23161 cgtctgaaca ggtgctgggc gcgcagcgcc tgctccaggt agcccagtaa ccgcgcctgc
    23221 tcgggggcga tatcttcagg tcggtcaggg ttgcgcagtg gctggttgag tatctcttcc
    23281 acgtgggcgt cgccaatttc agtcagtgaa acctggtggt ctttctcgtt aacctcgata
    23341 tcttcggggt tgagggcgcg caccacctgc gccatacgca catagtcttc cgtttcatct
    23401 gacgccggtc ccgaaatgat caacggtgtg cgcgcttcat cgatcaggat gttgtcaact
    23461 tcatccacaa tggcataata atgccctcgc tgcacacgtt cttccaggct catggtcagg
    23521 ttgtcgcgca ggtaatcaaa gccgaattcg ctgttggtgc cgtagacgat atccgctttg
    23581 taggcttcac cccggtctac cagcgcgagc atgtgcaggt cttcgcgcgc gttgggtttt
    23641 tccaggtcca ccaaaaaggc tttgcgacca ttttctgtgc gggcagccat ctgcagcaca
    23701 cctactttca accccagcag gttgtataca ggagccatcc agcgcgcgtc acggcgtgcg
    23761 aggtaatcgt tcactgttac caggtgaacg ctgcccccgg tgagcgcgtt aaggtataaa
    23821 ggcagcgtgg cgaccagggt tttcccctca ccggtgcgca tttccgcaat cttgccgtga
    23881 tgcagggtca taccgcctat catttgaata tcgtaatggc gcagcccgag cgtgcgcttg
    23941 ctggcttcgc ggacagcagc aaaggcttct ggcagcaggt catccagcga ctcgccttct
    24001 gccaaccgtt gcttgaattc gcttgtttta tgattcaggg catcgctgct caggccctcg
    24061 aagaccgttt cgagctggtt gatttcgtcg acgatctgcg agagatgttc aatttcgcgc
    24121 ttgtgagggt cacccccaaa gacacgggca attttttgta agaccatgtt cacctcttcc
    24181 agtcaggagg tgattatagc atagggtatt ttcgtttcag ctcagtggaa cgcctttcag
    24241 tatatgctgc aggatgtcag taaagctggc gccaagggcc aaccctatag cgacgaagat
    24301 acaaccgaag acaataccga tgagcaaatt cgccacgaag tacaagcccc acactgaaaa
    24361 ggcgcgggtg agaaaattgc tggaaaggat acccagctcg ggcatggact gacggctggc
    24421 tggcggtttc tccagctctt tttcaattag ggcaacacga gtaacgaggt tggtaatgtc
    24481 ctggtctgtg tacactgttc ctcccgataa tcctgtaatc tggtttttct atccgtgctg
    24541 agcgtcacac catccgattt ctatcaaata tgaatggctt tcccctcgac catttcggcg
    24601 gcttccatca acacttcgct taacgtgggg tgggcatgga cgttacgggc gatatcgcct
    24661 gctgtcaatc cgttcagctg agccagggtt aactctggca gcaattcaga gacctccggg
    24721 ccgatgagat gggcgccaag aagatgacca ctgtgttcat ccaccaccag cttggcaaac
    24781 ccgccgtaat caccgagacc cagggcttta ccatttggct ggaaggggaa tttccctatc
    24841 ttcactgttt taccgagcgc gcgcgcctgg gtttcggtat agccaaaaga tgccacctgc
    24901 gggtgagaat aggttgcccg cgggatcatg gtgtaatcca ggggtgtaac cgccaacccg
    24961 gccagcgctt cagcacagcg gatgccctgg gcagaagcca cgtgggccag cagcagttta
    25021 ccggtgacat cgcccaccgc ccagatattg ggcacgctgg tggtgagttg atcatttacc
    25081 ctgatgtgct tgcgctctgt caattccaca cctatttctt ctaacccaag gttctggctg
    25141 ttgggtttaa acccgatcgc caccagtgct tgctcggctt cgatgacgct ttcgccattt
    25201 tgaccgctgg tgctcacccg cacgccaccg ggagttgttt caacagccaa tacccgggtt
    25261 tcggtcaggc aggtgatacc gcgttttgaa aaggttttag ccagttccgc gctgatctct
    25321 tcgtcttcca gcggcgcaat ccgcggcagc atctccacca acgtgacctt gctgccgtag
    25381 gcgttccaga tggtggcgaa ttccaggccg atagcgcctg ctccgatgat caccacggat
    25441 tgcggcagtt tctcctgcaa gatggcttct tgataggtca gtaccttaac accgtcaatg
    25501 ttcacgccta ggagagaaaa aggtgtgctg cccgtggcaa tgataaaatc cctggctttc
    25561 aattcctgcg cggtgccgtc cttcccggta agggtgacgc tttcacgaga gctaaacctg
    25621 gcttctccca tgtacacggt gatattgttc ttcttcatta aataagccac gccgcgtgtg
    25681 agcctgtctg aaacctggcg gctgcgttta acggcggagg taaaatccag ctgcaggtta
    25741 tccattgaaa agccaaattc tcgcccgcgt tcgcgcaaag ttagcgctac atccgcgttc
    25801 ttgagcaagg atttcgatgg aatgcagccc acgttcaggc acacaccgcc cagccagcgc
    25861 ctgtcaatga tggcggtctt ttttcccaat tgcgcggacc gtatggcggc ggtgtacccc
    25921 ccgggaccgg cgccaatcac aataacgtca tactcttcca tggtgacact cctcgttggt
    25981 tgatgagttg attatacttt ttcccgcagc accctgaccc aggaagataa cccggtcagc
    26041 catcttcgcg ggatatggga cacggctggt gcacaagcag ggcgcaaaaa aggacaacag
    26101 tcctatgatt tcctgaaaga ggacttttta ctggtgaaga cgttgtactt gatgatgtac
    26161 cataatgcca tcaagccgtc tgtggcgttg atctttttgc cctcttcgta agtccgaccg
    26221 taataagaga tgggcacttc atacacacgg cacttcaact tggctacctt cgccaccact
    26281 tccacttcga aaccgaaacc ggtcgaagtg atgaccatat tgcggatgat gtctccgcgg
    26341 aaggctttat agcaggtttc tacatccgtg aggttaaggt cggtaaatag attagaaagg
    26401 aatgtgatga gccggttggc catgtagtga cggaagtaca acgagcgcga ggcgcggcgc
    26461 accaggaaac gcgagcccag cacaacatcc gccttgtctt cttggatggg ggcgaccaca
    26521 tcattaattt ctgccgggtc gtattccaga tcagcgtctt gcacgatgac gatttcaccg
    26581 ttggatcgtt tgaacccttc tttcagcgct tctgtctttc ccatgttgcg gggctgctgg
    26641 taataatgca ccaggggatt ccgggcttcg taatcgcggc tgatctgacc ggtgttgtct
    26701 ttcgagccat catcaatgat gatcacttct ccaaggttgc gcactttttc cagcaccagg
    26761 tccaacacct gcgagatggt tttttcttcg ttatagactg gaatgatgat cgagatgcgc
    26821 atgacttttc cttatccctt cctggcagat gagattattt taccccaatt acgcagatcg
    26881 acttcccgcc ccgctgccca ggcgcattgc gctgtcagga tggcgatcca cgcgggcgaa
    26941 ccagcgcatc agtcatgcgc gccactcgcg ccatcacttc aacatcgtgc acgcggataa
    27001 tatccgcccc gcgggcaatg ccaatggcgc ataccgcggc cgtgccctcc accctttcca
    27061 caggtgacag gtccagcgtg taaccgatga aagatttacg agaagccccc agcagcagcg
    27121 gatagcccag ggctttgatc tcacccaggc ggttcaccag ctccaggttt tgctccacgg
    27181 ttttcccaaa tccgatcccg gggtcgagga tgattcgccg gtcatcgact cctgccgcgc
    27241 gcgccagcgt cacgctctcc agcagttcgg cgcagatatc ttgcagaaga ttggcatatt
    27301 cgatgccgat atagcgcccg ccaagccgct gttgcaactt ggcgttggct ggcttgctgc
    27361 ggttgtgcat gagaatgatg ggagcctgat aacgggcagc ggttgcagca atcagcgggt
    27421 cagcgcgcag cccccataca tcgttgatcc aatctgcgcc tttttccaat gctgccgctg
    27481 ccacaggcgc tttataggta tcaatggaga gcagcacttc accgcctgaa ctcctgatag
    27541 cctcgatcac cgggatgaca cgagccagct cttctgcctc attcacagcc tccgcgccgg
    27601 ggcgcgtgct ttcgccgccg atgtccagaa tatccgcgcc ggcttcaatg aagcgctgcg
    27661 cctgctctaa cgcggcgcgt acggtgtcgg gctgtgagag caaaccgtca cctgaaaaac
    27721 tatcgggggt gacgttgaga atgcccatga catacgtttt cttgccgaat tctggaaggg
    27781 tttgttggcg catttttccc tcgttctcgg gtgctggcgt cacgaagatg gaaaaagcga
    27841 gatgccagtt gtatccaggc tggacagcac ctcgttgacg gatttaccgc tgccgggaat
    27901 gatcaggtca ggagagagct ctgccagtgg gagcatcacg aacgcccgtt caagcatacg
    27961 cggatgcggc acctgcaggc gggcagagcg gatcacccgt tctccataga gcaaaatatc
    28021 cagatcgatc acgcgcggcc caaagcgcag ggtggctttt ctacctaggt gtttttcgat
    28081 gttcttcagg ctgtcgagca gtttcaacgg cgggagggtg gtgtctccgc gcagtacctg
    28141 attcaagaaa agtggttgtt cgagcacacc ccagggaggt gtctcgtaca ggcgggagca
    28201 gcgcatttcg gtgataaatt cggacagtaa ctggcgggcg cggtacaaaa actcagcgcg
    28261 gtcgcctaaa tttgagccta gcgcgatata gacggtctct ttttttgcca ttgcgcgcct
    28321 gtgttccttt cagccgccgc gcagcgcgtt aatcaggtct gccagcagcc catacgcggt
    28381 ggtttccggt ccggggttgc tttccagaat gcccagcccg ggcagcgtat ccatttggaa
    28441 ctgnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    28501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnngcccgta cgcgacagct
    28561 tcctcaaatg tccgcccgct ttccatcatt tccagcagca ggtttgtgca ggagttgaga
    28621 ataccttgaa aaccttgcag ttcgaccgcg ggcagcgctc cacggaagag tgaaaagata
    28681 ggcccgccat ccatgacggc ggattcaaac aggaagcggc gttgtttttg cgctgccagg
    28741 cgggtcagct ctgtgtaagc gtgcacgacc gggcctttgt tggcggtgat ggcgtgcatg
    28801 ccgcgctcca gggcggcgcg caggtagctg gcggcaggct gaccgctttg gtggttgacc
    28861 ggcgagtttt caaacagcac atccgccggg acgctggcga tgaaatctgg caccgatgcg
    28921 ggggctggaa ctgcagacag cgcgctgata gcttgccctt tttgcgcgca gcgcagcgcc
    28981 tgcggggtgt caatcccttc ggggtcaatg gccaccccat ggtgcccggt ggcgatacct
    29041 gtcacctggg tcgaaaaacc aaattcagcc cgcaagcgct cttgtttgct ttccagcaat
    29101 gccatcagcg cctggccgac gtgaccaaac cccacaagcg ccacccggat cacttccatt
    29161 cgcggctcct gtgcattcgc cgggtcttca tgcgcgctcg catacttcgc catctcgcca
    29221 gaagaccctg ccgacagggt gtttttgctg aagcagaaga tacagggcgt cttgaaactg
    29281 gatggggttt cctgaggtga taaagcgtgg aacgctcact tcttgacctt ctaccagcag
    29341 gttgtgcgct tccagcattc ggcgcagctg cctggcgatg gctggcgctg gatcgatcac
    29401 gcgaacctcc tccccaacga tttgttgaat aagggggatc acaaaaggat agtgggtgca
    29461 gcccatcaca atcgtatcca ctcctttttc catcattggg gttagagcgc tctccagaat
    29521 gcggcgcgta gctggaccat tgagatcccc tttttccacc tgggctacca gccccgggca
    29581 agggtcttca aagagagtga ttccctgacc gaagcgttca accaccgagg catacatgga
    29641 agtgtgaaat gtggcttctg tagccagcac gccgaccttg ccgctgcggg attgttctac
    29701 agctggtttg acagcaggtt ccatgcccac aaatggaatg gcggggaact cctgccgcag
    29761 cgcgtgcagc gcggcgacag aagcggtatt acaggcgacc acaataagct ccacgccctg
    29821 cctgagcagg tattgcgtga tgccgcgggc aaacgcctgc acttctgtga gctggcggcc
    29881 gccgtagggc acatgtgcct ggtccgcgaa atagatcaca ggctgagccg gcagcgcggt
    29941 gagaatctca cgcagaacgc ttagcccgcc cactcccgaa tcaaaaaccc ctaccagatg
    30001 ctgcgatata ttcaatcagc gaaagtcctt tttattgtga agccgcgtga aggaatgccc
    30061 tgaaaattgc gcggtgttct tccatcgcct gcagcgattc aggatgccat tgaaccccca
    30121 ggcagaaacg ctgccctggg tgctcgattg cttctattaa accatcactt gcctgcgctg
    30181 tcacctgcag ccctatgcca gtttccctca ccgcctggtg atgccggctg ttcaccctga
    30241 gctcactgca agcagtcgcc cggcccagct gtgaagcagg ctcaatggtt accgggtgaa
    30301 tgagtttgtt gggcggcacg tcattgggct ggttatgcgt gatggatgtg tcatactgtc
    30361 tgggaatatc cgtgaataat gttccgccaa gcgcggtatt caacatttgc agcccgcggc
    30421 aaattcccag caatggccaa tccgtcttta ctgccagttc gaccagcgct ttttccaacg
    30481 cgtctcgttc ttttacaatg ctgttcgcgt aagggtcggg cctcgcctgg taaagcatcg
    30541 ggtcaatatc tcctcctccg cttattaaaa tgccctgcag caccccgcgc agcacctgca
    30601 accgatccag cgggtattca ttgggaatca atacggggca gccgccggca aggtgtacgg
    30661 cgttggtgta ttcgcttagc agcatgttgg ctttgcgttt atggctccga gagatctggc
    30721 tgacggtaat gccaatgaga gggggtgcca tgggcgctcc ttgggtcatt tgtatagaaa
    30781 agggcgcaac cacggcgttg gttgcacccg gaagtatgcg aacgaagatg gatcagttaa
    30841 atttttgctt aaggcgcgcg ctcttgcctg aacgatcgcg caagaagtac agccgcgagc
    30901 ggcgcacttt gctgtggcgc tccacaacca ctttctcaat gcggggcgag cgcagcagga
    30961 aggttctttc aacaccgata ccgttgctgg caacacggcg tactgtaaag gttgaatcat
    31021 taccgctgtt gtgctggtaa agaacaactc ctttgaactc ctggatacgc tcacgattgc
    31081 cttctttgat tttcacatgt acactgacca tgtccccggt tctgaggctg ggaatatttg
    31141 gattgacggg ggcatcgtat gcttttaaga tatcactcat tttacacctg ctttttttga
    31201 tcattcgccc tttaggcgcg aggaaaaatt ataacacgct tttaaaaaaa atggatatca
    31261 ttctaccagg agcgccagaa aaaaccttgc tatgaacttc tggtaaaatc aacggagaaa
    31321 aaaagcagcg aggatactat ggatgatata gcggtggata tcggcggtac gcagttgcgt
    31381 gcggcccttt atcctccaga gggacttgaa gccagggtga ttcaatcgat ccctacagct
    31441 ggggaaaagc ctgcgcttga gcggttgttc gatctgattg cctctatctg gcctgaaaac
    31501 ggcgcggtgc ggagcattgc cctgggcgtg gctggagccc tcaacccgca aagcggcgtg
    31561 gtgtaccagg tgccgaatat tcccaactgg aacggtctga atatcatcga gctgacggca
    31621 aaacgcttca atgtacctgt caggcttggc aatgatgcca acctcgccgc ccttggcgaa
    31681 tggaaatacg gcgctggcgt tgggcatcac aaccttattt acctcacgat ttctaccggt
    31741 gttggagggg gcatcattgt caatgaccgc ctgttgctgg gcgaaaacgg gttaggcgcc
    31801 gaggtggggc atatcaccgt gctgcctggc ggcccgctct gtggctgcgg gcatcccggt
    31861 cacttggaat ccatgtcatc tggcaccggc atcgccaatc acgtcatccg tcaaatacag
    31921 gctgggcgga aaacnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    31981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctatga
    32041 ccgccaggtt gaggttttcg agtaaaattg ggagcaatca acctaaagac aattggagag
    32101 acctggtaaa tatgatgcgt aagtcgatgg ttcttttttt tgtattgggg gtctttctct
    32161 ttctgctcac tccttctacc gcgtttgccg acacgctcca accggctgct cctctcgagc
    32221 aaatccaggc gcaggctgaa agcgaagtcg atatttactt tttctggggc gagggttgtc
    32281 cgcactgcgc gaaagccaaa ccggtgcttg aggatctggc aaaaaaatac cctgcagcac
    32341 atctgtatga gttcgaaatc tataattctc aggccaacca ggagctcttc tctcaatttg
    32401 gcgaggctta cggttttacg ccgcgttatg ttcccacgat tttgattggc aaaaatattt
    32461 gggaaggtta ttctgatgaa ttttcgcagg agatcaccgg tgtagttgag caatgccttg
    32521 aaaaagcgtg cgaaaacccg ggcagcgcgc taatcgcggg gaaaggctat gtcccgtttg
    32581 gcgaaaatgc cgctgcgcca accgctgtgc ccacatctgc cgcgccgact ccggcagcca
    32641 ctgaatcacc cgatacgaac acggaaaaca acggcgcaac ctctaaacta ccgatttttg
    32701 gtgaggtgga cttcaccaaa atcggacttt ttctcagcac ggcgctcatc tcctttgttg
    32761 atggttttaa cccctgttcg atttgggtgt tgagcatgct gctggcgatc accctgcaca
    32821 ccggttcacg taaaaaagtg ctggtcattg gtttggtttt tgttaccgtc accgctgcca
    32881 tttacgcgtt gttcattgcc gggttgttca ccgttctgaa ggtggtcagt tttgtgggtt
    32941 ggatacaggt agtggtgggg ctggtggcgc tcttcttcgg cctggtcaat ataaaagact
    33001 acttcttcta taaagaagga gtatctttta ccatttcaga tgagaagaag cctggcctct
    33061 tccagcgtat gcggaaagtg atggatgccg gggactcatg gtgggggttg atcggtggga
    33121 cgatcgtgct ggcggcgggc gtttcgctgg ttgagttttc ctgcacggca ggttttcccg
    33181 tcttgtggac gaacctggtg gctgcgcatc aggtctctgt gatcactttc atcctgttgt
    33241 tgctcctgta tttggtgatc taccagctgg atgaactggg catctttttt gtggcggttt
    33301 tcacccttaa aacctccaag atggaagaga aacatggacg catcttgaaa ttgattggcg
    33361 gggtattgat gctgacactg gccattgtga tgctggtaaa cccggcggtg atgagcgata
    33421 tcggaaaatc attaatcgtt tttgtgatcg cgtttggcgt tacgcttctc atccttctgg
    33481 tgcatcgggt cattttccct cgcctgggcc ttcacatcgg tacagaattc aaaaaatctc
    33541 aggatagaaa tggaaaacat aaagcgcatc ggcattgatg cctgtaatgg gtgcgttacg
    33601 aacactctct tttacactac ctcctaactg gaggcgggca acgaacaaac tgtgatagac
    33661 tgggtttgcc gcatgcccat ttgaagagcc gctgaaggtc aggtttggtg gagtttttgt
    33721 aagccctgaa ggcgttgaat gaaccctatt gataaagaaa gcgcgctgtc ttaccaccgg
    33781 cttggcggag taccagggaa actggtcatt cacccaagca agccaattga gaatcaacat
    33841 catctaggac tggcctatac ccctggtgtg gcgtaccctg tgctggaaat cgcccgcgac
    33901 ccggaactgg cgtatgagta caccaccaaa ggaaacctgg tggcggtcat cagcaacggc
    33961 accgccgtcc tcggcttggg caatcgcggc gcgttggcca gtaaaccggt gatggaaggc
    34021 aaagcggccc tatttaaaaa attcgcggat attgacagca ttgatatcga aatcgactct
    34081 gttgaacccg aaacggtgat cagcgtctgc gctgccattg gcaattcttt tggcggtatc
    34141 aacctggaag acatccgttc accggaatgt ttttcaattg aagaacaatt aaagacgatg
    34201 ctgaaaatcc cggtctttca cgatgaccag catggcacgg ccatcatcct gggcgcggcg
    34261 ctcatcaacg cgcttgaaat caccagcaag tcactcacag aagtgaaagt cgttttttca
    34321 ggcgcaggag cagccgcgct cgcctccgcc agccatttga tgaagatggg tattccccgt
    34381 gaacatatct ggatgtgtga tatcgccggg ttggtctacc gggggagggt ggaggagatg
    34441 ttcgctgaaa aagaggtttt cgcgcaagga gacaagcccg cctcgttagc ggaggtgata
    34501 gaaggcgcag atgtctttat cggtctttca gcggccaata tcgtcacccc tgaaatgctg
    34561 cggcgcatgc gcgctcaacc tgttatcttc accatggcca accctgaccc tgagatcagc
    34621 tatgaacttg ctcaacaggc gcggcctgac gcgctgcttg gcactggcag gtcagattat
    34681 cccaaccagg tgaataactt gctgtgtttc cccttcctct tccgcggcgc gttggatgtg
    34741 cgcgctacta ccatcaatga cgagatgaaa gtggctgcca gccaggcgct ggcagcgctg
    34801 gcgcacgagg aggtaccggc ggaagttctg aaggcctacg ggctgaagcg cctggaattt
    34861 ggcaaggatt atgtgctgcc caaaccactc gataggcgtg tatgcctttg ggaggcgccg
    34921 gcggtggcgc aggcggcaat gcgcagcggc gcggcacgct tatcgttgga tttgaacgac
    34981 tatcaaaagg cgctcgcgga gcgcttatat catccatgaa tcacaggagg aagtatggca
    35041 gagaaaaaac cactgggttt tcgcgcaatc agggtgaacg ctgaggccag ttcgctggac
    35101 aagtggcgta aacaggtgat tgccggtcag cccgaaaccg gacgggctta cgctttcatc
    35161 agcgatgagg gcacctacat gccgggaggg gaaggtactg ctccttctcc gctgacgtat
    35221 ttcgtcagcg gaatggcgct ttgactgctt tctcatgtgt cgcaggttgc gaccaaaaag
    35281 aaactgcaaa tccgtaacga aagagtgaag gtcagcgcgt attttcatga gcagggttcg
    35341 gtgttaaaag gtgacgctga ggggtattgc gaccattttg agatagaggt ggtggttgat
    35401 tctgacgcgc cggaaaaaga aatccaaaaa ttgattgacc ttgcccgcca gatgtgcttc
    35461 actgaaaaag ccttgatgga aaccgtgaag gtggaggtgg ctcacaccat caacgggaaa
    35521 acccagccgg caaagaaata gcgtgaaata aatctgctgt ataattcaac caggtcgatt
    35581 ctaaaagcga ggtattcaat tgatacagat aacgacaccc aaaaccaaag aggattttaa
    35641 agcctactat gatctgcgct ataaggtatt acgtgaaccg tggggtcaac cacgcggcac
    35701 cgaaaaggat gattatgaac ccatctcaca acactttatg gcggtagacg atgaaactaa
    35761 agaagtagtg ggatgtgtca agttttctga aaaagaaccc ggggttgcct gggtctcgca
    35821 tctggctgta aaaaccgatt tgcagcgcac gggaattggg cggcagttat tggcgcacgt
    35881 tgaagcccag gcgcgtgaac agggctttaa gaagatcggc tgccaggcac gcttgaacac
    35941 gaccgcctac tttgagaaag cagggtacaa aatgcacggt ctgcctaccc ggtattttgg
    36001 cacgacccag gtggtatgga tggagaaaga gctctaaccg cgactgcctg ttgataaaaa
    36061 accacccccg tgtgttgtac tcacgggggt gtgttcttga ttattcagcc acgctgggag
    36121 tagcccgttg gcgaagaaaa agtttgctca cgagcgtgaa aatgcctttg atgggagcga
    36181 agatccaggt gatgataccg ttgatcaaag cgaacccctg gctcaaccag atcaataatg
    36241 ggcggaagaa tttcataaac ttgaaacgct cataagccag ggcgagcaga atggtggcga
    36301 tggagatacc gaagcgcgtc caatcaccaa tttcaatatg gccgaggtct tcaatgagca
    36361 gctccacacc gatattgagt accaggatgt aagccgcggt ttttaagacg ggggcttttt
    36421 caaccacttt gctgaagagg ctggctgcaa aacgcatcat tacaatacca attgccacac
    36481 ccagcatgac cacccacagt ttttttgaaa gtgacacggc tgccacaacg ttatccaggc
    36541 tgaaggccag gtccatcaat tccaccgcca gcacagtggc ccagaaggtg cgttccttta
    36601 ccgggcggtc accatcttca tcgctgccac tggcgccgtg cgcgctcagg tcatccaacg
    36661 ccagtttgat caggtaagcc gctccaacca gttttaacca ggggttttga atcacataac
    36721 tggctgccac cagcatgatt cctctgcctg cgtacgcgcc gatcaagccg actttcaaag
    36781 ccgcggtgcg ctgaggtcca aggattttgt tcatcgcgtg cccaaaccca gcgagggctt
    36841 ttggccaggg gatgggttca tggtcagaca gcggtgtgac cattgcgccg agaacggcag
    36901 cattatcaat ggatagaatg ccttcaagga agatcagttg aaggataatc acgataaacg
    36961 aaaaatccat tttttctcct ttaataactt ccgccctgtt ggcacagggg gaaagataac
    37021 cttcgacgcg caaggataat gtacattgag agcggaaata ataacactgg attattaccc
    37081 taatattggg gatatttgtc attaagacga aatatcaaca cgttttcttt agcgaatgcc
    37141 cgcgtaaact gaagaagaaa ttcagtggaa ttgtgatata ttataaataa gatagtatat
    37201 atctaaataa cgagtgtaag atgacacttg caggtttccc catcccgtta cccccgcaac
    37261 tgagttttct ggcaaaaccc ctgcctgtgt tcctggtgaa tattgccgcc tggatggtat
    37321 tggcggcttt tatttactgg gtggccatgc ctttgatcag gcgcttcacc ctcgcactgc
    37381 ctggagaggt ggaagatatc gttctgggaa tcatccgccg cccgctagcg ctgctggtgc
    37441 ttgtcatcgg tatacgcgta tcgattcgct atctgcaggc agcggaagat ttgcagcgcc
    37501 tgttgattcg cctgtcgatg acggttatcg tggcgctggt cggtcatatc gctggtcgac
    37561 ttatccgtga tgtgctggtg tattatggcg aaagctgggc acggcgcacc gagagccagg
    37621 tggatgacat cttgctgccg gtgctcagcc tctttggccc gttgctcctt ttcaccatcg
    37681 gcgcattgat cgtattaccc atgtggggtg tagacgtcag ctcagtttta ttgggggcag
    37741 gggtgttggg gctggtgctg ggtctggcgc tgcaagaaac gctttccaat attttcagcg
    37801 gcctgagctt gttaatcgaa gcgccattca agcgtggtga cctcatccga ctggtgagcg
    37861 gcaaagtttg cgaggtacag cgtcttggtt tacgctctac caccctgctt tcattagacg
    37921 agcaggcgac gatttacatg ccgaataagg tcctggcggc tgattacctt gtcaacctca
    37981 ccaagccgac tgcggaacaa cgctgcagtg tttcggtcag cgtggaggct tcgaacgacc
    38041 ttacgcttgt taaagacacg ttgtaccgca tcgcagccgg ccaccctggc gtggtggctt
    38101 ccaacctggc aaaaaaaacc gcctccatcc tttcacaggc ggaaaaaatt cgcgcgtggg
    38161 cagggggact ttctgcggag gaccccaccc gttttgaatt ggagaaaatg gctgcggata
    38221 atgaatctgc catcgagcgc ctggctcgcg aaggcagggt gaatgaccag cttgtccttc
    38281 tcaaggaaga gattcgcaat atgatcagga gcatccgctc aagagagacc agcgggctca
    38341 gccgcgcaga agtggccgag attttgaacg agcatgtgaa ccctgtggat aacacagtga
    38401 ggacgctggc agagcaatgt cgtgactggt cgaccacgcc tgatcgctgg ctgaatgatc
    38461 atgactactg gcagcagcgc cggctttggg attcacgtaa cgaagaattg ctgcgccatt
    38521 ggaacaacct caagaactgt attacaaacc ctgatgaacg cgtagagtta cgcctggatg
    38581 atctttcagc ggttctgatt gactggctgg ataaagaata taagatcgtg cccggtgaat
    38641 ggaaagaccc tatggtagaa atcaagaatg taagcgcaga ttctgctgaa cttgagctgc
    38701 attactatgt ggataatatc cgcttggaga tggatggtcg ccccaggcgc atccgttcag
    38761 agctcaatca cttgatcaaa catgaattcg ttcaagaagg tatctggagt tgagccaggc
    38821 gaaacttcat cccgccagga gcgcgggcgt ggggctttca acgcgctgtt cacctgtttt
    38881 cgcgaagagc gatgatttcg tactataatt ttgttatgaa agatacagga aggagtaaaa
    38941 aatgaatatt ctgatttgga ttctggtagg cgcgatcgcg ggttttctgg ctgactgggt
    39001 ggtaaaaggg attcggatgg ggttgctcgg taagatcatc gtcggtatcc tcgggggatt
    39061 cgtgggtggc tggcttttta gtctgctggg aatcaacctt ggaaatggca ttcttggcgc
    39121 ggtcatatcc gctttcgttg gcgccgttat cctgcttatg ctgcttctca tcatccgccg
    39181 ccggcgctaa cccgcttttg ccttcatgca tattccccgg gggttgtacc tgacggggaa
    39241 ttttttatcc agcaacgggt cagtccttga acaggggggc gggcctggcg agcagctttt
    39301 gataatcttc gggcgaatca acatcaaaag ccaggtcagg ttcttcaaac ggaacctgtt
    39361 tgaccgggaa ttgcgaaaac agcgcgcgtg cccctgcatc cccgctcagc ctggcaagcg
    39421 cttcaaagag ccgcttttcc agcagcactg ggttggcacg cttaccttga aaagtgggga
    39481 tgagtatggc ttccgcgggg tcatcctgtt gaaaaacacg cagcagctcc cgtggcacgc
    39541 gcggagggat gtgcggctgg tcacccaaaa aaaccagcgc cgcgtcgatc cctgctggca
    39601 gcgctttcac tcctgccttc actgaagtgc tttgcccttt ttgccaatcg gggttacgcg
    39661 taaagatcag cggcaaccct gcgagggccg tttgcacttc ttgcgcgttg gcgccggtga
    39721 ccacaagcac aggctcacaa cctgtaaaca gcgccagtcg cgccgtccag cggaccaggg
    39781 gttgaccatg ccagtgaagc agcaatttgg gctgcctcat gcgcgttgag ccgccagccg
    39841 ctaaaaggat ggcggctact ttcttgtggt cgtgccttcc tgtactatcc gtcatggcca
    39901 tgagcggtcg ccacgatttc agccataatg gataatgcga tctcttccgg ttgacgcgcg
    39961 ccgattttca accctaccgg cgcgtggatg cgtttgaggt cttgttcttt ggcgccttcc
    40021 gcccgcaggt cttctaatcg tttggcgttg gttgtgcgtg aaccgagcgc gccgatatac
    40081 ctggctgggc tggtgatggc gaacagcagc gccgggttat ccagtttgtc atcgtggctc
    40141 aaggcgacca cgcaggttgc agcatccagt ttcaactgtt gcagcacgtc agccggccac
    40201 ccgacgacca actcatgcgc gtgcggaaag cgttcacgcg tggcaaaggc ggaacgcgcg
    40261 tctaccacga tggtatgaaa ttggagggta ttggccagcg caaccaatgg aatggcaata
    40321 tgtgaggcgc cgatgatcac caggcgcggc ggcggaacaa taaaatccgc gaataacact
    40381 tctccatttt ttaatgtgat ttgcaaaggt aagcgtgtcg cccagttttc cggcaggttg
    40441 ccgatggctt cctggtcaag ttccctgttt cccaggctgc cgtgagtttc gccatctggt
    40501 agcaccaaca tttttttacc gacactgtca ccgtgaataa cggtaagcag cgcggcaagc
    40561 tggttttcag ccaatatgcg tgccaggggc ggcaggacag cctgccaatc gggcgtgtcc
    40621 agcgactcga ccaagatttg aactgacccg ccgcaagaaa gccccacgtc ccaggcttgc
    40681 tggttggtga cgccgtagct caataagcgc ggagctccgt ctttcaatac ttcgcgcgct
    40741 tcttcgaata ccgcgccctc cacgcagccg cctgtgaccg atccgtgaat tgccccgccg
    40801 ctggagatga ccagttttga cccttcgggg cgcaacgagg agccggtaac ttgaaccact
    40861 gtagccaggg caacctgttg tttttctgcc tgccactgct gtacaacctg ccagatttct
    40921 cgcatgtgat tttcctcctt cagtcaatca atgtctgcct ggtgtcacgc atgagttaag
    40981 ccttccaggg tttgggtgtg actaccgtgg agaaaagcgc gtgctgtttc caggtcaggg
    41041 attcctaacc gtccgccggg gcgcgtggct ttgagcgccg cgctggcatt tgccagggcg
    41101 accgcgtctg gcaggctgtc tccgcgggca agggcagccg cgtaaacccc atggaagaca
    41161 tctccacagc cagtggtgtc tacaacgtct accttatgcc ccggcacatg cactgcggaa
    41221 gcaccccgtt gcgcgtacca gcagcctttt tcgcctgcgg tgaccacgca gcaggcgcgt
    41281 tgcgtaccgc ataaagcgaa cgccatggct ggttcgctgc tttcaccgct caatgtttga
    41341 gccagtttgc tgccaatgat caggtgatcg accaatggca gcagttccac cacctctggc
    41401 ggcgggtcgg gttccacatc agcaatcacc ggtatgccaa gtgaagcggc aatacgcgcg
    41461 gctttgatcc ccgccgggcc tgagttgtca tccacgaaca acaccctggt gcgggagatt
    41521 aactcggcgt taatgagctc tggcggtggg ctgacattcc ctccgctgtg gaaaatggtg
    41581 cgcgtgtgtt tttgcgtgtc aacaatgatg atggaatgat aagggctgcc ttgcctgtcc
    41641 tggtggcagg ctgaagtctc cacgccttct ttttgcagtt cgctcaaggt aaaacgcgaa
    41701 aggtcatcaa gccccagccg gcaacagaag gccgcccgag cgccctgcct ggcagccgca
    41761 accatggcgg tggctgtttg cccaccaccc tggcgcatgg attcaaggat gtttaccttg
    41821 ctgtcggctt cagggtaacg ctccaccagc agcaggtcat ccactgcggc agtcccaatt
    41881 ccaaagacat cccaggtgag ggtcattcct tcttcctttg ctgtcgataa tactaccata
    41941 acccgccctc gtggttgctt aaaaactgta tacgatgtgc tgacctctcc acaacccctt
    42001 tacaatagct gtttgacttt ctttttactc tctactaaaa tatttttaag agagttgaaa
    42061 aactcatctg ttctggttgc aagccagaaa aatgatttcc aaaaaaaagg agagaaaatg
    42121 aagaggatta cttttatttt gtcagttgta atggccttcg cgctgttgct gtcagcgtgc
    42181 gccaagccgg ccccaaccga agcgccatcc gtccctgagg tccctgctgc cacagaagca
    42241 cccgtggtaa cagaagcacc cgcagcgccc gaagaagtga cgcttgtggt ttgggatcag
    42301 ttctatcgcg acgttgaaag tgaagtgatg gaaacgttga acgcggaatt tgaagccgcg
    42361 catccagggg ttaagattga acgagtggtc aagacactgg atgatctgaa agtgaccctt
    42421 aagctcgcgc tcgcgcagga agatggacct gacgtggctc aggtgaacca gggacgcagc
    42481 gacatgggcg ccatggtgca ggctgacctg ctgctgccgc tcaatgatta cgccgctgcc
    42541 tataaatggg atgagcgttt ttcttcatcg gtggcaagcc gcaacagctt tacccccgac
    42601 ggtgtgacgt ttggtcaggg aaacctgtat ggtgtgagcc ccaccgctga ggtagtgggt
    42661 gttttttata ataagaccat catggatgcg aatggctgga cgcttcctac tacgtttgat
    42721 gagttccaaa ccctcctcgc gggtatcaaa gacgctggaa tgacacctat ttcgtttggc
    42781 tcactggatg gctggaacgc cattcacgag ttcagcgccg tgcagcacct gctggtttct
    42841 ctggattaca tcaacgattt cgtctatgga gtgaacaatg tcacatttga cactccagaa
    42901 aaccaggaag cagcacgtgt gatgcaggat tgggttgaca agggctattt cagcccggat
    42961 tttgctggca ttggctatga cgacagcaat aagctcttta aagccggagt tggcggcgtt
    43021 ttgaccatca ccggctcgtg gctggcgggt gagctggttg gcgacaccga tcaggaattt
    43081 ggattcttcc tgctgccggc gaatcccggt catacccggc tggcgattgg cggcgtgggg
    43141 attcccttct ctatccgcaa gaccactacc aaggccgacc tggccgcgga atatctggat
    43201 tggatgacca gcccgcgcgc ggccgaattg tgggccaacg ctggcatgct gcctgccatg
    43261 cctttgccgg aaggttacgt ggcggagggc aacccgctgt tcaacgatac actgatcgcc
    43321 tgggacacga tcaactcaga gaacgctgtt ggtcattata ttgactgggc gacacctacg
    43381 ttctatgaca ccattgtggc ggaactgcag aaactgttcg gcaaagtcat cacgccggcg
    43441 gaattcacaa aagccgtcga ggtggattac gccgccttct tggctggcaa ctaatcctcg
    43501 ctgacgttcg cccgcttctg ccaggttggg gaagcgggcg aatttttcaa caggagcagt
    43561 ggtgtgaaat cccagcgcag atggccgggc gaatcccggt ttcacggttg gctttatctc
    43621 ttcccgggtt tgcttatcta cctggttttt gttttttatc cgatcatcga aaccttccgc
    43681 accagctttt ataaatggga tggctttagc cgcgagcggg ttttcgtgga ctttcaaaat
    43741 tacatttcac tggtcacaga tgcgcagttt cttaaagcgt tgcttaacaa cttggttttc
    43801 atcatttttt acagtattat ccccattctt cttggcttgt tgctggcctc attattaggg
    43861 cgtaagccat tacctggaat gacatttttc cgcaccgggt tattcctgcc gcagattctc
    43921 agtatggtgg tggtgggggt tacctggcgg tggatcttta acccgaactt cggcttgttg
    43981 aatgtcgggc tgcgcgccat tggtttgggg gcgttcaccc gcgcctggct gggcgatttt
    44041 caactggcgc taccttcagt aggtgcggtg ggtacctggg tgcagtacgg cttttgtatg
    44101 gtgcttttcc tggcgggcat gcagcgcata cccaatgacc agtacgaggc tgccgagctg
    44161 gatggcgcca acgaattccg tcagttgctg catattacac tgcccagcct gcgcgctgag
    44221 ctgggtgtcg cccttatcac caccattatt gccgcgttgc gcgtttttga cctggtttac
    44281 gttaccacgc gcggtggacc gggggattcg acactggtga ccggcttttt ggtttaccgc
    44341 tcagcgttcc aacagaatca gctcggttac gcttcaacgg ttgccacggt gatgacgctg
    44401 ctcatttttg gcatttcgct gatcatactg cgttttcaat ccagagatgg ggagggggta
    44461 tgaagcgcca ggcctggcag cgcctgctgc ccaattacct cattctcagt gcctttttgc
    44521 tcctcatcct gttgcccatt attggcctgg tcttcagcgc ctttaaaacc gacatcgagg
    44581 tgataaaggg accactcaca ctgccctcgc agcttagatt cgcttccttc aaagaagcct
    44641 ggaccacagg gcgttttaat ttcttcttcc gcaacagcgt gattgttacg gttgtggtgg
    44701 tggtcatcag cgtcttcctt tccacgctca cgggttacgc ttttggcatg ctgccggtgc
    44761 cgggtaaaaa ggtgctttac ccattgttgc tggtggggta tatggttccg tttgaggcgg
    44821 tggttattcc gctttatcat atgatggata acctgaagtt gacagatacc tactgggctt
    44881 tgatattgcc gcagattggg ttgagtgttt cattcggcac gttgtggatg agcagttttt
    44941 tcagcacggc gccttctgaa ctggtggatg ccgccgcgat tgacggctgc aatcgctggc
    45001 agaccctgtg gcgtattctt tggccgctgg cgcgcccggc ggtgctcact ttggtggtgc
    45061 tgatctttat gtggacctgg aacgagttcc tgctggcgct ggtgatgatt caaaaagaga
    45121 ccatgcgtac cctgccggtt gggttggcct ttttccaggg gcgttattcg gcaaatatcc
    45181 cgctgatggc ggctggcgcg ctgattgtcg ccgggccaat cctgctcgtt tatattgtgt
    45241 tccagcgtta ctttattcgc ggcatgcttg gcggtgccgt taaaggatga gcagaatggc
    45301 ggtggaggaa taaatgaatc agaaagaaaa aaacctggtc aatgaagcgg ccaaacacgc
    45361 gcttgaaata ctgcatcagt gcgtcactcc gtacggtttc cgtgcttcag cggatccagt
    45421 tgggtatccc caggtatgga gtcgcgatag ttcggtcacg ctcttaggcg cgctggccag
    45481 tgaagataaa gagctgatca acgctggcag ggcttcgctg gatgcgctca gcggcgcgca
    45541 atcctgctgg gggatgatcc cgcttaacct caaccctgaa agcggggata tcagcacgga
    45601 gaatgccggt tcctgcgatg ccaacctgtg gtacatcctg gggcagtacg cgctttaccg
    45661 cgccacaggt gataaagacg cgctggcgcg cagctggccg agaatcacca gcgcgctcac
    45721 ctggctgcag taccaggata tgaatgaatg cggtttactg gaaattccgg aagccggcaa
    45781 ctggatggac cttattgcgg tgcgctataa caccctgtat gacaacacgc tgttttacgc
    45841 tgcctcgctg gcgtatgaag agatgctccg ggataaccct gcggagctgg ctccgctgcc
    45901 ctgtcacatc agcgctgatt ccatccatga acgtattaat ctgctcttct ggattgaccg
    45961 ctgctgggtt gcttcgcact ttgccgaaca cctggagaaa ctgaaagcca ttcgactgga
    46021 gtggttcatg ctgtactaca acattggcac catttccagc cgcccatttt acctgccctg
    46081 ggtagccttc cgcgaatatg gagactggtg cgacagtctg gcgaacctgc tggctatctt
    46141 aaccggtatc gccgacgggc accgtgctca acacatttta cgctacatgc agcaagtggg
    46201 aatggctgag ccgtacccca ctaaggcaat tcacccacct atttaccccg gagagacctc
    46261 gtggcgtgag tattatcgca gccgtaacct caacctgccg gatcaatatc acaacggcgg
    46321 catctggccg atggtcgggg gttttcatat cgccgccctg acgcgttgcg gctggcaagc
    46381 tgaggctgac aagctgctgg tacagctggc gctcgccaac cagcagagtt ctggcgcgcc
    46441 ctgggagttc aatgagtgga tgcatgggcg cactggtcac ccaatgggat acgctcacca
    46501 ggcctggtcg gcggccatgt acctgtacgc ctggaaagcg gtgaatggtg cggagctacc
    46561 gctgtttgat gacctgcttt ccgccaaacc tgccagagcg gtggcagaag aagtgaacac
    46621 cccgtatttt catcctggcg gcgggcaagt ggggtagccg gtcgggtttt ttgcatggat
    46681 tgcggcgcga aatcttcagc agcgtgccgt cagtcagcgc gacccccgga tattaccgtg
    46741 aataagcgat gatgaaaaaa gtgatttggc tggttctcat aggcatgacc gcgatggtgg
    46801 cnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    46861 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ngcggctggt attatgatga
    46921 accgggcagg gtgttgatca agacaggggt attggatgtt cacgtgaata agcacattga
    46981 aatacaatat gaatcgtagt cccgctttta gggcgttatt cgaggaaccg gatgggtaaa
    47041 ggtagattaa gaataggcgt gatcggttgc ggcggcatcg cgcagatgat gcatctgcca
    47101 tttctacaaa agaacgctga ccgctttgaa attactgcgc tttgtgacct gtcaaacaag
    47161 gtgctttcag cgctggcaag gcgttaccac ctgcctgata ctggatgttt cactgattac
    47221 cagctgatgc tcaaacagaa ccttgatgct gttttggtaa ccagcggcgg cgatcatact
    47281 ccgcaggtgc gggcagcgct gctgtccggc gcgcatgtgt tcgttgagaa acctttatgc
    47341 tacacacagg cacaggcgct ggaactgggt cggttagcgc aagaacaaaa acttaggtta
    47401 atggtgggat atatgaagcg tttcgacccg gcctaccagc gcgcgcgtca actgctggct
    47461 ggcttgggag aattacatta cgtgcagctc aacacactgc accccgagga ggagggctat
    47521 ctgtatttcc agggcctttt ccaggctgag gatatcgcgc cggaaacgat caggcactta
    47581 cgcgcgcagg aagaccaggc catcaccgct gcggtcggtg agatacccgc ttacctgcgc
    47641 gccgagtaca cggatgtgct gctgggcagc atggtgcacg atatcaatgt tttacgcggt
    47701 ttattgggag aaccgcagaa ggtcctcttt gccgaacatt ggccgcaagg tgaaaagcct
    47761 ggcggaataa ccactacgat gatgttttca cctgacctca gggtggttta tacctgggta
    47821 ttcctcgctg aattgagaga ttactctcag gaaatcgccc tcatgtctgc cagccagcgc
    47881 ctgcgcttgc agttcccttc gccttatctc aggcattttc caacctcgtt aacccatcaa
    47941 tacatgaaag acggtgcagc ggtggaagag aagatcaccg tgaattacga cgaagccttt
    48001 gagcaggaat tgttggcttt ctatgactgc gtggtgaacg accgtgaacc tctgacgggt
    48061 gctgatgatg cacgcgcgga tattgcggtt ttgcagcaga tactggcagg tttgcacccg
    48121 gttgggttat cgggggaagc tgccggttat atgctgggaa ggaattgaga tgcgtattgc
    48181 ccttcacggt tccagctttg tcgccagtca gtttggctac acacctcctg gcagttggga
    48241 ggagtgcgtc aacgcgctga gcgcgtttta ctctccgcct gttacatttg aagcacggtt
    48301 tcaagggctt gtcgcgcggg tgcgggaatt gggatttttt cgcatggatg tatggcagcc
    48361 agcggaactg aattggcaat gggctgggcc ggagcatatt gcagcggctg taagagcgct
    48421 gcaacacgaa ggggtgtcgc caacaagcct ggcgggtgaa ttcggcgaaa cccgcgccga
    48481 atttgagtcg gcctgcgccc tctccgcggg gattggcgcg ccgctgctga gcggtaccac
    48541 cgcgctttat cacacggata gagcctttgt gattgaaacg ctcaaacgtc acggactcag
    48601 gctggcgatt gagaatgagg tagaattgac cgctggtgag atgctggaga taattggtga
    48661 cggtgggaac ggggtcatcg gcacggcgct ggatactggt tggtacgcca cgcacgggta
    48721 tgacccggta caggcgattg atgagctgcg ccagttcatt ttccacgtgc atcttaagga
    48781 tgtgttgccg ggcgatgcgc atattaactg tgcttatggc aaaggcattg tcccaattga
    48841 ggcgtgcgtg aaaggtctgt tgaaaatcgg gtatgatggc gttatcagca ttgaaaacca
    48901 cacgcttgac cacgatccga ctggagaatt ggtgcaggcg cgcgaatggt tggaaagcct
    48961 tataaaatca aacacaccac tgaattgaaa gaggaagaat gagcctgatt gatgagattc
    49021 acgaacaacc gcaagcgatt cgtaaattac tcaattcaca gggggaagct atcgcgacca
    49081 ttgcagggca ggtgagagcc cacggaattg aatacatata cctggccgca aggggcactt
    49141 caaagaatgc cggcaggtat gcccaatatc ttattggcgc ggtcaaccgt ttgccgatcg
    49201 cgctggcacc tccctctttg ttcagtatct accatcatcc gccgctgctt aagaatgctt
    49261 tggttattgg cgtgtcacaa tctggcaggt cgcccgatat tatcagcgtt atagaagaag
    49321 ccaggcggca ggaggtcgtg tcgatggctg tgaccaacag ccctgtttca cccatggcag
    49381 acgctgccga gttcattctg gatatcagtt gcggccctga agtggctgtg gcagccacaa
    49441 aaacctacac caactcactg gcggccctgg ctttgttctc cgctgaactg gctgaaaacg
    49501 cggaaatgaa acgcgcattg gagaatgtgc cggcctggat ggaagagatg ctggggagag
    49561 aggatgagat cgcgcaacag gcagccagtt tttgctctat gaatcactgc gtggtactgg
    49621 gccgagggta caattacgcc accgtcaacg aatggtcgct taagatgaaa gaactgtcag
    49681 gcgttatggc tgaaccctat tcatcggcgg atttcctcca cgggcccatt gcgatggtga
    49741 agggggactt cccggtactc aatgttgcgc cttcgggcat cgcttacccc tcactttatg
    49801 agctgctggg acgcctgcga gaagagcgcc gggcggaaat ttactccatc acagaccggg
    49861 acgaggtgct tcagctgtca cgtgccgggc agcgccttcc gcaaggcatc cctgagtggc
    49921 tgaccccgtt gattgccatc atccccgggc aattgttcgc ttaccacctc acctgccata
    49981 aagggttgga tcctgataac ccctccgggt taagcaaggt gaccgagacc cattaaagcc
    50041 acctggattt gtgtctggca gagaatggtg cgcccactct ttaagataac agagggagga
    50101 tgtacctgtc cacatcctct tcggaggacg gcgagggaat agaacggaaa agcagccatt
    50161 cttcactatg gcttacgctt tgaccaggct gcaattctgt cagcggcgcg agcgtttcca
    50221 attccagaat atccctatct gtgtaggctt caaagttcac attaaaatcc gggtattcgg
    50281 cattgggctg ccaggcgaag cgcttaagga acagttgacc gttgagcacg taggcagccc
    50341 aaccgtacgg attttgcagg ccaaatttct gcggtgtcgc ggaggatggg tcctatcagt
    50401 ttgaatttgt gtttgaaata ctgtccgcct tttaatccca ttccccccgc tcacggatga
    50461 taaaagtcat aaaaaaccct gagaattata attatgttgg gaaaaaaa
//
LOCUS       DAOTLW010000010        50029 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_831_length_50029_cov_9.766399, whole genome shotgun sequence.
ACCESSION   DAOTLW010000010 DAOTLW010000000
VERSION     DAOTLW010000010.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 50029)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 50029)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..50029
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_831_length_50029_cov_9.766399"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(143..1000)
                     /locus_tag="P5516_02895"
     CDS             complement(143..1000)
                     /locus_tag="P5516_02895"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HRT91419.1"
                     /translation="MILVTGAGGKTGRAVIQALISRGAGVRGIVNSYINAQKITALGA
                     EAVLGDMGSLEDMIGAAEGAAAIYHISPNVHARELEFGQTAVAAAIKAGVPRFVFHSL
                     CHPQIESLPNHWLKLRVEEKIKESGLNFTIFQPTPYMQNVLGQRQNILERGVYEIPYQ
                     PSTLLGMVDLDDVAEVAARVLLTPKTYDWATYELAGPEILSQTEVLHIIRLVTGKEIA
                     LKVISREDWCARAASTGMPQYAVSIFYRMFEFMESSGFWGNPGVLEWLLERKARRFEA
                     WFRAVGNWS"
     gene            1165..1860
                     /locus_tag="P5516_02900"
     CDS             1165..1860
                     /locus_tag="P5516_02900"
                     /inference="COORDINATES: protein motif:HMM:NF027640.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4956 domain-containing protein"
                     /protein_id="HRT91420.1"
                     /translation="MTIIDRLAGLLDRILISGQDIGDVLFSVLLAAVMGLAISRIYKW
                     THRGLNYEHTFMATLVLLAPIVSIVMLYIRGDLVLSLGLIGSLSIIRFRTPIKDTRDM
                     VFLFWVIAVGLGAGTYNWAVVGVTTPIVAVIVAALYFFRYGHSPNTDFVLVVNGSGEY
                     RKEEIENVLEKYTTGKRARSFDVDGEKWEVIYELRFTKREKQPVEGMVAEMKEMSGVN
                     KVSVLAPQLALPL"
     gene            1885..2628
                     /locus_tag="P5516_02905"
     CDS             1885..2628
                     /locus_tag="P5516_02905"
                     /inference="COORDINATES: protein motif:HMM:NF020916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate polymerase domain-containing
                     protein"
                     /protein_id="HRT91421.1"
                     /translation="MKPSARLDEKNWRFEYKYRLSVEQYLRVRNQIRAYMEIDEYTER
                     AAEKKYLVCSVYYDSLGLDAFNEKIDGNSDRIKFRLRTYCRELSQGTTIRAEMKVRKN
                     AITEKYGAFISGAEYEMFIGQKRWPKIRNQVMEEFERYVHLKGLNPLLLTEYRREGYR
                     SRAREEIRITFDHRVKSAHSRTLFPEAPHFHEHHPGLIVLEIKCIKQQPGWLRRLVQQ
                     NGLMIMANSKYVQGMMVAYPALVTPGWSA"
     gene            2668..6111
                     /locus_tag="P5516_02910"
     CDS             2668..6111
                     /locus_tag="P5516_02910"
                     /inference="COORDINATES: protein motif:HMM:NF020337.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CotH kinase family protein"
                     /protein_id="HRT91422.1"
                     /translation="MKSKNLVPVSATLIAGLCFAYSLVVRFSHFLLVSRGEKAITLGV
                     HWLWIVFAGLLFILPLVNRLLEGISKERLKIIGGLSLVTAVVIMAVFYSLPAFPKRME
                     LEIRAKGEELAVAGKGITILSMVRQDPPKNIPTPINLRNLSYGGGWQVMEGKLRFSPG
                     QGDSGSVRYTSNIQGEVRIRFQTGPGEGAVDILVNGEGQSVELAEAEEGEVEKTVTIP
                     NTWKNADRVRRVLLMAGLLTDTVTVFLSAVMFWLLVYQVVVKRRLRARGLADVVGLVL
                     VSLVLLFANQQAQREIVFSDPKLEEGVREALGKPEGRLYTHQLLTLIELDLSGRGIRE
                     LEGIEGIRNLRVLDLSDNQIENLTPLQGLKKLQKLNLRENRVRDITALAEMKQLTYLN
                     LHSNEDIQDLTPLKNLTMLNSLILRNVPIGDQVSILTNLNRLTYLNIRHTSIRNIEVL
                     VEMMSHGVLQDDVSAGKRVTVNILENEIGEGKGDPFLGLRGYWEKVSYRYPYYLPNYA
                     SEVAGPKFSHESGFYEEGFALVLTTPEAGGKIFYSTDGSEPAIDEKRMAMGSTREYTG
                     PITVNDKSAGRNRLSQIETSRIDDYKPPENMFKAMVVRAVVVGEDQRRSGVVTHTYFV
                     EEGVKERFSLPVLSLTVAPVTLFDDRYGIYVPGELYQNDHPSNQLHNTANYTQRGLKW
                     ERPAFVELFDPSGEVVFAQEIGIRIHGRASRGYPQKSLRLYAREEYSGESTLQFDFFP
                     GLNERLNRLEVDKFETLILRTGGAGDMEDTMIADIIGQSLLENLQLDIQGNYPVIVFL
                     NGEYWGIHNLRSRYDAGYFQNYYHIPPGDVVVLVGSESELYLGDPGDENLYTDMLRLI
                     DEHYDTNGFRTVSTLADAQAYAAMSELMDMENYIDYNISQIYPNKVDWLGNNIWYWRK
                     KVDCALPESGEMYGHDGKWRWMVIDMDSGFKDRYFNSMENVTAEGSQGTYLFRSLLQN
                     EEFRRSFINRLADLLNTNFKTEVVLATIDEVQRVYEPEMEEHILRWGMPGGSLAGWRA
                     NVEEIREFARARPEELRQQIADYFDLAGSAELRIRTDPGKGYVTVNRLEIREGMVGIE
                     DPSNWAGVYFQGVPFTITAVPAEGYRFSHWEGLKGGDSTAQSQEITLTGDLALTAVFV
                     R"
     gene            6405..8729
                     /locus_tag="P5516_02915"
     CDS             6405..8729
                     /locus_tag="P5516_02915"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRT91423.1"
                     /translation="MKFKYLGKSYNRPDAISKVTGKAVYLDDIRIPGMLHAAILRPEY
                     AHAEIVRIDKTEAEKMPGVVKVVTGENCHYHYGDNFKDLVPMAVKKVRYIGEPVAAVI
                     AETLAQAEAALEKIKVEYKPLPVYIDARDAMKPGAALVHEENGEYWHLPTLKPTPGTN
                     IAHTYHLKKGKGVDGFKDADVIVEGEFNYPLGSCAAIEPHGSIVWFKEDKTIEVWSSS
                     ICPFIIRDDLAHSYGVPVSDVRVRIPDIGGCFGYKSDITVEQTVAWIASFVPGHPVKW
                     VASRKEDFTSTLVAHGIRSRMKIGARKDGKLVAIQNEVLHTGGAYADTAVNITIAATH
                     NSAGPYEFEHCDLTGYTVYTNTPPVGAYRGYGHQEAQIATERMMDLLARKLNMDPIKL
                     REMNYLVEGKVNALGEKMWKSHGDLMTCSNMVKEKVFSKPKPAEDENYYYGRGFAAVM
                     KSPKGAPFSTKSCYMKMNIDGSISVSMGGAEVGQGLRTVVQQVTAEALKIAPERVRVY
                     TEIDTQYSPYEWQTIGSMFTTQGGRAIVRAADKLIAVLKNTAAQVLKTDVDYLEYDGD
                     YVYLRNDPDIRVAVADLSRGYITSDGITIGEYAHAVSDARLPRYANPDENGQGSLGVN
                     YTFGATAAEIRIEKKTGKIIVDKFTSVFDVGQIINPKQIRGSVMGGVTMAVGAALYEK
                     LDISEDGKINNPQLFKYHIPTYKEAIPMDIEFIETPDAVGPFGARGVGEHSVIGPAPA
                     IMNAIRDAVGIDFFEIPITPDVMKKALEARQEVK"
     gene            8729..9205
                     /locus_tag="P5516_02920"
     CDS             8729..9205
                     /locus_tag="P5516_02920"
                     /inference="COORDINATES: protein motif:HMM:NF013923.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRT91424.1"
                     /translation="MAEKITFTINGKLTKVEVDPEMLVIDVIRDVLNLTGTKRGCDNS
                     VCGTCTIVVNGKATKSCNMPIKKLAGADVLTVEGLAKGTELHPIQKAISESGAIQCGF
                     CTPGIVMELYGLLEEKPDAAQSEIENALSKHLCRCTGYEPIRDGALMAQKLIKERK"
     gene            complement(9254..10009)
                     /locus_tag="P5516_02925"
     CDS             complement(9254..10009)
                     /locus_tag="P5516_02925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014635485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HRT91425.1"
                     /translation="MKALQVQNLSVRYSDNNESLKNVSMEIESRAITVLFGPAGGGKS
                     TFLRTLNRLNDLADVKEIKGRVLFHQRNILDPEEDVITLRRKIGMVFSRPVPLPLSIF
                     DNVAYGLVMAGEHNQARLGEKVEKALKQAFLWNEVHDRLNQSAFSLSGGQQQRMCLAR
                     VLALEPEVILLDEPTSALDPIVTSSIEDMLRELKKEYTILIAPHSVQQAARLADYAAF
                     FLMGELIEYGSGIEIFQVPKDQRTSDYITGRFG"
     gene            complement(10014..10877)
                     /gene="pstA"
                     /locus_tag="P5516_02930"
     CDS             complement(10014..10877)
                     /gene="pstA"
                     /locus_tag="P5516_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HRT91426.1"
                     /translation="MKAAHLARAKMAQTIGFHLLRLVTLITIIPIIGLVVYIVVKGAP
                     AISWEFLTAAPSNGMREGGIFPAIVGTLYLTLGTALFSVPIGVLAGIYLAEYAQETAF
                     TRIIRIAIINLAGIPSVVYGLFGLGLFVLFLHLGTSILAASLTLSIMTLPVIISTTEG
                     ALRSVPNSFRVVSTSLGATRWQTIRRITLPQALPGIITGIILGLERSAGETAPILFTG
                     ATFFLPQLPRSPLDATMALPYHIFVISTQIPGMPIQIQYGTVLVLLVFVLGMNLIATL
                     IRSRARAKRQW"
     gene            complement(10877..11773)
                     /gene="pstC"
                     /locus_tag="P5516_02935"
     CDS             complement(10877..11773)
                     /gene="pstC"
                     /locus_tag="P5516_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HRT91427.1"
                     /translation="MKAREEKDGLVINGVIRISGYSSILFVVLILFFLLREGLPALTN
                     IPLRSLFSIRWYPIEGYYGILPLIFGSLLITLASTLIALPFGIGTAVYLSEVAPPWLR
                     DLLKPIVEILAGIPSVVLGFIGILVFAPLFRKMLNIPTGLTAFTGAILLAFISIPTIV
                     SVAEDALNTVPHSYRQASLALGATQWQTIWDVTLPAAKTGVLTAVMLGVGRSLGETMA
                     VMMVTGNAPLMPLHLNSLFRPVRTMTATIASEMGEVASGSLHYQVLFFIGITLFLLSL
                     VINITASLVSTKARQRSERLLS"
     gene            complement(11784..>12491)
                     /locus_tag="P5516_02940"
     CDS             complement(11784..>12491)
                     /locus_tag="P5516_02940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRT91428.1"
                     /translation="NARISVSGGGSGTGITSLISGTVDIANASRRITEEELAAARQAG
                     FDPQEFIIARDAIAIIVNPRNLVSNLTLQQLSRIYRGEINNWSELGGEDREIVRLSRE
                     TNSGSHLFFLESVVRMGEMDNNAIFSASTLLLPSSEGIIAEVRDNPNAIGYDSLGYIT
                     SDVKLVGISSLEEPMNFILPSLETVSNHTYLLSRNLYMYTLDEPEGLVKTYLDWIMGP
                     EAQEIAHKLGFMPVLNE"
     assembly_gap    12492..12591
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            12651..15170
                     /gene="gyrA"
                     /locus_tag="P5516_02945"
     CDS             12651..15170
                     /gene="gyrA"
                     /locus_tag="P5516_02945"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HRT91429.1"
                     /translation="MDFGTVHQVDIDEQMRSAYLDYAMSVIISRALPDARDGLKPVHR
                     RILFAMADMGIRSNTAYKKSARIVGEVLGKYHPHGDSAVYDAMARMAQDFSMRYPLVD
                     GQGNFGSIDGDAPAAMRYTEARLHAHAEEILTDIDKDTVDFTDNFDGSLKEPVVLPAR
                     LPNLLLNGSSGIAVGMATSIPPHNLRELANATNYLIDHYEKIDEVSVEELMRFVQGPD
                     FPTGGIIVGRDSVHQAYSTGRGRLVVRGQAHIEEMKGNRYQIVITEIPYQVNKTSLIE
                     RIAELARGGRLDAITDLRDESDRRGMSIVLELRRGAQPKQVLNQLYKYTPLQSTFSVH
                     LLALVNGEPRLLSLKRALQIYIEHRQVIITRRSSFELEKAKNRAHILDGLLIALANLD
                     DVIKTIRESKDAEIAKERLMSKFNLTDIQAQAILDMQLRRLSALERQKIEDEHREIMA
                     RIEYLQDLLAHPQKMLGLIKEDMNAVSEKYGDERRTKIVGETHEELKEEDLITDEPVL
                     ITFTRKGYIKRVAARLYRSQGRGGRGVSGQTIREEDEIEFLIPARTLHTVLFFSDRGK
                     VYSEKVYQIPDANRTDRGIPIVNVLSLDTGERITAAVSVPGFEEGSYFTMATLAGRVK
                     RVALADFSSVRPSGLIAISLDEGDELGWVRLTSGKDEIIMVTRAGQALRIPETEIRCM
                     GRPAAGVTGIKLKTGDRLTSMDVIEPEGQLLTVTEHGFGKRSDLDEYPVKGRATGGVA
                     TIAQKQTARVGRITSARVVQEEDEVTLISSGGVILRLKVKDISMSGRATRGFKLMDLG
                     DEDVVASVARIQTSDITKTEGDSLPLENGKAETPGEEPAGV"
     gene            complement(15258..15476)
                     /locus_tag="P5516_02950"
     CDS             complement(15258..15476)
                     /locus_tag="P5516_02950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91430.1"
                     /translation="MLDDLRNSVNSAYEEELIEEEHIRGGRYTIERPPFLGMSPFQRF
                     IVALVFFLMVAILGILLLVVFQKIMPPI"
     gene            complement(15466..16278)
                     /locus_tag="P5516_02955"
     CDS             complement(15466..16278)
                     /locus_tag="P5516_02955"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HRT91431.1"
                     /translation="MERHEIAETLRGTAVSAWHHFESIGSTNTEAQCWAESGAPDFAL
                     VLAEQQTSGRGRFDRRWITSPGASLALSLILRPTPDEVPRLALFAPLCGIAVHETVQR
                     VCGLSPQIKWPNDVLLKGQKFCGILVESSWQGSQPAAVIMGIGMNINRGSIPPPELRG
                     FPATSLEAEYGSPVDRAAILRELLFSIHAWRGKLGTPVFMQYWEEHLAFKGQQVRIEN
                     SERPLIIGKIKGINELGQLLLINDSGEMVPVTVGDIHLRPVQPDDPGGNNVG"
     gene            complement(16357..16776)
                     /locus_tag="P5516_02960"
     CDS             complement(16357..16776)
                     /locus_tag="P5516_02960"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRT91432.1"
                     /translation="MTLYIRTPEMIAESRRRMMRRMLENTFNQERVLSFPLEMKVNEN
                     EYTLTALLPGLSTDEINIQFNNGTLSIDGEYQEIRDEKSEYLFSEMPAGKFSRSVEIN
                     DPIVSEKISAGMKDGVLTIHLPKAEEAKPKTIKINAK"
     gene            16925..17245
                     /locus_tag="P5516_02965"
     CDS             16925..17245
                     /locus_tag="P5516_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91433.1"
                     /translation="MEDKITIIEGPPPVFEKIDDGWALGLTESSAYYGMALTRLRTFN
                     GPALVERCHRRWNRQEPIFLHYRNEMGLEQQVPILAARASEVDEGQVLFLWIRYPMDS
                     HGDE"
     gene            complement(17412..17485)
                     /locus_tag="P5516_02970"
     tRNA            complement(17412..17485)
                     /locus_tag="P5516_02970"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(17449..17451),aa:Arg,seq:cct)
     gene            17548..18798
                     /locus_tag="P5516_02975"
     CDS             17548..18798
                     /locus_tag="P5516_02975"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017743.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRT91434.1"
                     /translation="MSLSKENTQFELEILDFAYGGNAFSRLPDGRPVFVPFTMPGERV
                     RVRVTTAFKDYCTAQLEEVLKPAPGRVAPRCQHFTVCGGCHYQHLPYQQQLEVKQRIV
                     ADQLRRIGKITAPAVNQIIPSADEWQYRSTMQFHISKNGRPGFRAGDGKTVIEVNECY
                     LPIKGIDKLWPRLDIDAQSGIRRVMLRAGSDSELLAALEGESNLPPEFNLDFPLSVHY
                     LSSDRDYLLAGDAHNLMTVNGVNFMVSPRSFFQVNLGQACKMVNYVLANVDLNSAVSA
                     LDLYSGVGLFSAFLAPRVKELIAVESSASACDDFAANLDFYDNVSLYVGGAEQVLPGL
                     RIAPDLVIVDPPRAGLMFKVVDYLVNSPVTQLVYVSCDPATLARDCWRLTQGGFTIRS
                     IQPFDFFPQTYHVECVVFLDKKRG"
     gene            19004..19963
                     /locus_tag="P5516_02980"
     CDS             19004..19963
                     /locus_tag="P5516_02980"
                     /inference="COORDINATES: protein motif:HMM:NF018190.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0016052 - carbohydrate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HRT91435.1"
                     /translation="MKKVIKLTTAFLVLSFLLSSCNLPFFRGDAAEENNPPNLTLTAL
                     FDTSMNIPPSMTPALPTSVEVIQTTPAIVLPTLAGSDSPTPNPIPTNTSAPPTAEVRQ
                     RSGKLMKAAYLSTPPVMDGTYAEWVDKTNKYAIPYVVWQPSNWKNMEDLEGAFAAGWD
                     DKYLYISVKVTDDVFAQNKSGESLYLGDSVEVMIDANLDGDFYVNYLNDDDFQFGISP
                     GNPAKGIDPEVYLWYPSALKGSKSQVLVSSQFETSPIYRVEVGIPWSMLGVSPYNGLH
                     LGFVVSVNDNDKPSENLQQSMISTAQHRNFLDPTTWGELMLVK"
     gene            complement(20004..20837)
                     /gene="trxA"
                     /locus_tag="P5516_02985"
     CDS             complement(20004..20837)
                     /gene="trxA"
                     /locus_tag="P5516_02985"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRT91436.1"
                     /translation="MTSEHIISVTESSFEYDVIAYSQNIPVVVDFWADWCKPCRVLTP
                     MLERFAEQSQGEFRLAKVDVDANPNLALRFSVRSIPTVMAFSQGRKVSDFTGLVPEHV
                     VREFLARILPPRPSDLLAQKGNSLLHAGELKEAMEAFMQALDLDENHSPSLLGMIKIN
                     LLEGNSAAARSLYQNFPASPEFNAAEKLVPLIKALQDIDDDQLPQENDLDAVFVNSIR
                     LVKRRNTLAGIDGLLEVIRQDKRYRKDLARSVLLALLELLDPEAELTRKYRAELASIL
                     F"
     gene            complement(20881..21297)
                     /locus_tag="P5516_02990"
     CDS             complement(20881..21297)
                     /locus_tag="P5516_02990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91437.1"
                     /translation="MAAEPLFKGLISDEFDHPVDTAIVGSEPCYVIDDAGFKRHVPSR
                     PIDLEILRSLFKQVEGNEDIIAAQAAKMTGQDDIFSHAILEASLKNIDKQYEFLLDHG
                     IPEETRAYLGLMGFRVVLDIRGNILKLEYPSAPSSE"
     gene            complement(21377..22432)
                     /locus_tag="P5516_02995"
     CDS             complement(21377..22432)
                     /locus_tag="P5516_02995"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRT91438.1"
                     /translation="MAEALDLRNMSRREFLKLSSASILSLLAIHSTTYAYAQTRSLQD
                     TAAQLGRITTNKVDMFPTPSAEGKPLKTLWKDLVLPITRMEVSSSEPSHNRVWYQLDG
                     QGYVHSGSVQPVKISLNETRQDIPVKGLLGEVTVPFTDAIWNPLLKNIVAYRLYYSST
                     HWITGILQDARGDDWYEVQEDFYRFKYYVNPAHLRVIAPAEVEPISPEVNAQDKRLEV
                     RLKEQIVVAYEGDAPVQMLRCSSGTEYRNGYLTPTGSFRTDYKRPSRHMVNGSLASSW
                     GYDLPGVPWICYFTEEGVAFHGTYWHNDFGRPRSHGCINLFSEAARWLYRWTLPVVPY
                     EEQLASKVGGTRVEISI"
     gene            complement(22434..23594)
                     /locus_tag="P5516_03000"
     CDS             complement(22434..23594)
                     /locus_tag="P5516_03000"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HRT91439.1"
                     /translation="MNKQDFPAYRQAILNAQAALKNRDFAEVRQWAHQAAQIAPEREE
                     PWLLLAAASPPAVSLQYLQKALAINPTSEWATKGMRWALNRLARTSLEETPAAKPQPQ
                     SPAADETQPIRVRAAKPDGAQPASPRPIAAAPREKGELPGQPQPAKQAVTPGTRKPVL
                     RRKTQRKWLIFLLVAVLIALAAFVIWAIVPNWIALARSSAAPIPADALVKPSLTPTAT
                     ATPTATPTATPTFTPTSTFTPTATNTPLPTDTPWPTYPPAAQEPVAIRTDNEGHWIDI
                     NLSSQMLYAFDGDEMVASFLVSTGTWLHPTVTGDYQIYVKYRYTDMRGDDYYLPDVPY
                     TMYFYRGYGIHGTYWHNNFGTPMSHGCVNMRTSDAAWLYDFADVGTLVSVHY"
     gene            complement(23591..24451)
                     /locus_tag="P5516_03005"
     CDS             complement(23591..24451)
                     /locus_tag="P5516_03005"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HRT91440.1"
                     /translation="MSDDSLFDHVEPPFEPLPEEEESLLPESEEEEQDSHQPFRIWSS
                     IQSIVVTAFLLASLFTLFTPNNLFSGEMFDRIFQAWQANPRISAPVQVNTPATPALPI
                     GIVSGHYKNDSGALCPDGLTEEQVNLKIASLVQQKLMAEGYTVDLLEEFDDRLSQYKA
                     LALVSIHADTCDFISNEATGFKVAAAIHSVYPDKATRLTLCMADRYAQTTGLTYHPNA
                     ASEDMTYYHAFDEIHSTTTAVIIETGFLNLDRQILTENADLVAQGIVNGLLCFIRNEN
                     VTLVQPTPVP"
     gene            24595..25044
                     /locus_tag="P5516_03010"
     CDS             24595..25044
                     /locus_tag="P5516_03010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HRT91441.1"
                     /translation="MRRWPSGVAILTTSDGLTTHGLSVNSFTSVSVDPEMVTVTLANT
                     TRAKRIVDRTGLFAINLLPETQAELSDVFAGRIPEHADRFAGVETTPGLTQIPLLKAA
                     SAHVECRVVHTYEMPHSTLYVAEVLRAEKNADAPPLVYFNREYHRIQ"
     gene            25044..25613
                     /gene="amrA"
                     /locus_tag="P5516_03015"
     CDS             25044..25613
                     /gene="amrA"
                     /locus_tag="P5516_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HRT91442.1"
                     /translation="MTNPLTEEEKHYLLQLARRTIEQALKQAPEPEIDLGMLSAALKE
                     PGASFVTLTIRRNLRGCIGSLEARQALAFDVREHARQAAFEDYRFPPLSKAELPATSI
                     EISRLTAPLDLEYQQPSDLPRILRPGIDGVILQDGYRRATFLPQVWEQLPNVDDFLSH
                     LCVKMGSPADLWKRKTLQVKTYSVEEFHE"
     gene            complement(25647..25913)
                     /locus_tag="P5516_03020"
     CDS             complement(25647..25913)
                     /locus_tag="P5516_03020"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HRT91443.1"
                     /translation="MKMIIAIVNDDDSEMVTSALTHGDYRVTTIASTGGFLRRGLSTL
                     LCVAEDEKLENALALIRACFPETTDPSQKRCRIFVLNVVETHHF"
     gene            complement(25916..26263)
                     /locus_tag="P5516_03025"
     CDS             complement(25916..26263)
                     /locus_tag="P5516_03025"
                     /inference="COORDINATES: protein motif:HMM:NF017920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HRT91444.1"
                     /translation="MSEQPASTPNTMLMAVVQAQDADLVENILNDMNISLIRLPSTGA
                     FLGRRNATLIIGIPQEQQPQILNALHDNCRQRIEYIAVPLESAPLPLPAPTPVTVGGA
                     TVFALEIEHYEEL"
     gene            complement(26286..28601)
                     /locus_tag="P5516_03030"
     CDS             complement(26286..28601)
                     /locus_tag="P5516_03030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91445.1"
                     /translation="MLPSVIPQAICRFLCVDMRFGKPGILDDQCWAIGTELAEPKALS
                     LLTTYGLRASRMHVFPQFSMENLVIQDPARFSAYPVVSFSSTCLVKFTFSPFPSIDVE
                     YRVWVPHPQVVVGQMNCLNTGQALLKVVVDWRVHLQPLQGGSPMKHAQAGLNTIMQGE
                     CANLRPVFYLTGGAMASLTDFPGLGNQMLLMPGTSRQVTWALASLTSVESSTQQARQF
                     SSSALELEQIKIEMADKGSKVHCTSAQAALDDKLQHAQDRTHQLLMPPLQKHTQITYV
                     ANRSPDLGYHVSESILDAQPEWNGQTLPEIYLLAHNLLPGRPEALKGLLQNFLNLQQP
                     NGRIDLRASVNHTLTGHSSLPMLASLVSELQPYLDDFTWLRGIYPQLLAFLKTWVVLD
                     DSGSLVLSALTHPLQLGFNDHSPDTQADLVELWFRLAHPRNLFLVSLLYREVSELLRI
                     GRVIEMNDALDWLEQIRARLAEAGTDLLKPTDRMNHPSAPGTTRLVLASFHNDGFPRF
                     KQNLPHPGKICLRLDYQGRLPAELNGCLTGFAAGTYLEKRITAADFQPVGSSFCYVPR
                     EEFEFIESISVKRLPEGARGEISLVDQNSPSVFELMACYAGFLSEKDSRRITSRAKVK
                     SYLTDHGLAVFPVSEPAPGLLLPSYLAAMVIDGLVRYGKFDQADQLFQNHFLKDLGKG
                     PSDQGSRSRCSTSRITELAPTALFLRLHGLMKLSNRELVIRHYDGKQPPVTVQYNQLE
                     LLLKHHLTEIHLQSGEVLYLNQPGTSRITLE"
     gene            28687..32097
                     /locus_tag="P5516_03035"
     CDS             28687..32097
                     /locus_tag="P5516_03035"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRT91446.1"
                     /translation="MTDDVNTPADESAVPQKPIDPSLPNIGRFQNLPNPEEPPGYTWQ
                     FIQDRSHSQATVPVTPSNEEQVADSKTEPSSPHPQDEQNSQGPSGDTEPHRLRLRTSD
                     TGGEKAESEHFEDLLTVPSVSLPSITRKEEGYPPRKVIPPAGGRDGLPRKVDEVDLSA
                     TIATPAALQVNRTGEKAARKLPAAPPARAKIPAMNRRKPTRPRQQKKPGFDYGGCLVK
                     VVVILLFMLVVGVIFAGIVFVYQYYTIASTLPSVEDIRGHASQFETTRFFDRNGQVLY
                     EMIDPNAGRRTYTPLEDISPYVLAATIATEDKEFYNHPGFDLLALVRAMVTNYTSGEV
                     VSGASTITQQLARTLFFTPEERVEISYRRKAKEIILSSEITRRYTKDEILELYLNEIN
                     YGNLAYGIEAAAETYFNTTAKELTLAQAAFLAGLPQAPSVYDIFSNRDMTLNRNKQVL
                     TAMYNVSKEKDCIYVSNSPQRICIEAQQAADAYIWIDNYEFKLKANPMVYPHWVTYIR
                     YLLETMYDPQMIYRSGFRVFTTLDPHLQLEADRIVREQVEALADRHVTDGALVAIDPE
                     SGEILAMTGSADFYNEDIAGQINMALQPRQPGSSIKPLTYAAAFEKGWTPATVLWDVP
                     SEFPPSGDPNDTRDPYIPVNYDGKFHGPVSVRSALANSYNVPAVKALEFVGIYDNPAT
                     VEADGLIAFARRLGISTLVRNDYGLSLTLGGGEVTLFDMTSAFSVFANNGRRVVPVAI
                     TRIEDHKGKIIYESNPREPEQVMRAEHAYLITSILSDAMARAPMFGSDSILNLPFPAA
                     VKTGTTNEYRDNWTIGYTPDLVTGVWVGNADNSPMVGTSGVSGAAPIWNAFMNSAIPY
                     LTGNAPANFTRPEGIVEKEICVYSGAEPSENCTERRRELFAFDQPPPSSEDDLWKLLK
                     VDTWTNLKASSACEGFAETKLVLNVSDKWAKKWIDDTDQGRAWLEKINFSPPVIYVPS
                     RECRADDPRPTLLFVGLNDGQTITTDKLDIYAVVDATKNFKEYYLEYGEGKDPHKWTT
                     LVEPGGATSADPQKLLTWDLLKVNNGVVTLRLFMLSTKNGHAEKLLRLNLQVPTPTPT
                     NTPTPTETPTITPTPTSTATPTPTLTLTPMPLFPTLDAFLQTLLPPGN"
     gene            complement(32114..33331)
                     /locus_tag="P5516_03040"
     CDS             complement(32114..33331)
                     /locus_tag="P5516_03040"
                     /inference="COORDINATES: protein motif:HMM:NF017019.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HRT91447.1"
                     /translation="MKVRSITSFYDPACPQAERQLKRLAEVSEALREAIQQKLEVQST
                     RLGTSPFSCYLNDRPPAEVISCATQLEQKAQSLGWQYLTMGPALPEHPWAYKLIPQLI
                     RSTKNVFFSAVITANHTLYPGAVLAGAQIIHQTAQNSTDGFANLRFAALASVEPWTPF
                     LPAAYHQAGSAPAISIAVECADAVNDAFSQDLTLEECRQSLLDSLQNASSQLEEVILP
                     IIHSGGIRFKGFDFSPAPFPEDWCSLAGAAERLGLAHIGGLGTLPAIALIADTLDRGN
                     WRKAGFNGMMLPLLEDSILAKRAAEGQLNVKDLLLYSTLCGTGLDTIPLPGDTSIEQL
                     RSVLMDIAAISIRLDKPLTARLMPIPGKSAGDFTDFHFPFFANSRVLSLEGQPLHRFF
                     TSQDPIHICPRRR"
     gene            complement(33328..34131)
                     /locus_tag="P5516_03045"
     CDS             complement(33328..34131)
                     /locus_tag="P5516_03045"
                     /inference="COORDINATES: protein motif:HMM:NF012797.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HRT91448.1"
                     /translation="MRLALEVITSFKNPKVQRLRSLISQRSYRDETGQFVIEGVRLVE
                     EAFRRHFLPQSVYFSVNLSARGIQLVDRLRQTGVEAFEVSGDILNRLSDTETSQGILA
                     VLPQELISLTGVAEPVLIIDQVRDPGNLGTILRSAAAAAAGAVLLAPGSVDPFMPKVV
                     RAGMGAHFHLPICQTSWDGIEDYCKRQAQPPLQVLLADAGSGLPIWQADLTRPLALVI
                     GGEAEGAGKEARALSDAAIHIPMPGNFESLNAGVAASLLLYEVLRQRSA"
     gene            complement(34209..34559)
                     /gene="rplT"
                     /locus_tag="P5516_03050"
     CDS             complement(34209..34559)
                     /gene="rplT"
                     /locus_tag="P5516_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HRT91449.1"
                     /translation="MARVRSGFTSHQRHKRTLKFTQGQFGSRHRLFRRANEARLKSMW
                     YASRDRRMRKRDLRRLWITRINAGSRQNGITYSQLIHGLRKANILLNRKMLADLAARD
                     PQAFSAVVEKAKAA"
     gene            complement(34658..34885)
                     /locus_tag="P5516_03055"
     CDS             complement(34658..34885)
                     /locus_tag="P5516_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HRT91450.1"
                     /translation="MPHKMKTGKFKLKTHKATSKRFRLTGSGVLLRTKGGKSHLRRRT
                     SDRTKALFTEMVPVQGDKIIQRVKRLNPNMK"
     gene            complement(34999..35616)
                     /gene="infC"
                     /locus_tag="P5516_03060"
     CDS             complement(34999..35616)
                     /gene="infC"
                     /locus_tag="P5516_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HRT91451.1"
                     /translation="MSTQNFRVNELIKVAEVRLIGPQNENIGVVPIQKALQIAREAEL
                     DLVEVAPLSEPPVCRVMDFGKFLYERTKKEKEARKAQTKIEIKEIRLRPKTNEHHRGF
                     KTRDARRWLLEGNKVKVTIRFRGREITYPEIALEDLKEIAEELADIATIEQAPNMEGR
                     TMGMVLSPSKPGKKKPSPAPEKKDAAEDKANEVPPGEPQKTAEKA"
     gene            complement(35737..36411)
                     /locus_tag="P5516_03065"
     CDS             complement(35737..36411)
                     /locus_tag="P5516_03065"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT91452.1"
                     /translation="MLTAAHINPPIKTILRAFMRIDDSGIHSVPMSGPLIMATNHINW
                     LDAPVGFSHLHPRKITAFAKIETWDNYWLRLLFNAWEAIPISRGEVDFNAFRKAEEAL
                     AAHKIIAIAPEGTRSKTGKLNTGYPGIVFLALRTNAPIMPFVFYGNENIQLNLRKYRR
                     TPMVLKAGAPFRLKAQSSNPDKLMRRELTDAIMYEIARLLPEEYRGKYAEIDPGYRRH
                     LEFIDL"
     gene            complement(36411..37337)
                     /locus_tag="P5516_03070"
     CDS             complement(36411..37337)
                     /locus_tag="P5516_03070"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HRT91453.1"
                     /translation="MSRYLIILNPIAGKGKALRSLTHIKESLAQYNLEFDIKVTEYPG
                     HGTEIARKAAEEKWDYVIAAGGDGTANEVLNGLMQNRAAGGFLPVMGILPVGRGNDFA
                     FGANVPTELDEACTLLASLPRKWIDIGHVSGGDAPQGMYFGNGIGVGFDAMVGFMAAK
                     MKLTGMLSYLVAAIKTIFIYFKPPNVEIKFADKTLTLPALMVSIMNGRRMGGGFMMAP
                     SSSITDGQLNICMVREVPQFSMFGLMAKFIKGTHESDPAVSVARADRITIIAKKGALP
                     VHADGNTVGEACHEITVELLPHSLEIITRLEN"
     gene            complement(37523..37599)
                     /locus_tag="P5516_03075"
     tRNA            complement(37523..37599)
                     /locus_tag="P5516_03075"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(37563..37565),aa:Val,seq:gac)
     gene            complement(37673..38095)
                     /locus_tag="P5516_03080"
     CDS             complement(37673..38095)
                     /locus_tag="P5516_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91454.1"
                     /translation="MSSIVPDLELPILVVDDAHWQKVSSTGEEGEEYSIFGRDGFRLS
                     TKGYEFTIPSGVDFIAPNIIQLVIGKDQLYATAYEPDCTLYTIDPANLVPMYGSRRFT
                     GFQKGQKLIIAIGHLSPPGHDLPQPRFIVLWAGVVNIL"
     gene            38270..38356
                     /locus_tag="P5516_03085"
     tRNA            38270..38356
                     /locus_tag="P5516_03085"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38306..38308,aa:Ser,seq:gga)
     gene            complement(38707..39201)
                     /locus_tag="P5516_03090"
     CDS             complement(38707..39201)
                     /locus_tag="P5516_03090"
                     /inference="COORDINATES: protein motif:HMM:NF017774.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HRT91455.1"
                     /translation="MKKILIDGHNLIPKVGGISLSDPEDEAKLTARVSEYCRLAKVKA
                     ELFFDGAPPGKNATSRLGLINVHHVRQGTTADAAIIQYLHREGYNARNLLVVSSDHEV
                     QNKARMLHAEVMSSDEFARQMRAVFSSPQASMNIKETPPSEEEVEEMLKLMTENREEN
                     STTT"
     gene            39269..39751
                     /locus_tag="P5516_03095"
     CDS             39269..39751
                     /locus_tag="P5516_03095"
                     /inference="COORDINATES: protein motif:HMM:NF013125.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HRT91456.1"
                     /translation="MRKILPYLLLNFVVSALAVWIVLMVWEANHQIPNIPAAEQPVTR
                     ETKSPTITQPPLDEKTITIQLVMGSGDIRSERVQLVSASASPVNLQGWELVDEDKNRY
                     QFPSVTLFPGAAIELYTKGGVNTAGELFWNREEPVYTSGERILLKDTSGNIRSEYQVP
                     "
     gene            39846..41522
                     /gene="dnaX"
                     /locus_tag="P5516_03100"
     CDS             39846..41522
                     /gene="dnaX"
                     /locus_tag="P5516_03100"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HRT91457.1"
                     /translation="MTQAFYRKWRPRRWDQVVAQDHVVQTLKNAIKADRVGHAYLFSG
                     PRGTGKTTTARLLAKAVNCLNENPAERPCDSCSHCLAVNENRFLDLIEIDAASNTSVE
                     DVRDLRDRINYSPTDGKYKVYIIDEVHMLSTAAFNALLKTLEEPPPHAIFILATTEVH
                     KIPATVLSRCQRHEFRRIPVAETVKALREICKEEQIETDEEALLLIARQSTGALRDAI
                     SLLDQLASTGKHIDLALAQTVLGTATNQLVFDLIEGLLKNDPALGMQTIHKALDGGSD
                     PRQYARQVVEYLRNLLLMKMGNEKEIEATREVKNRIREHSDRVEIDALMEWIGLFNAA
                     VSDLRTTWQPSLALEVAFVRALNPAGAERVPVLETGTEKQQSRSTPAAEKIATREMAA
                     AEKKAEKKNEPPAAEEVADTPAPLPEEPAARVQIEQPGKVHGKEMAGAGSAELTTQDL
                     LASWSAIRAEVKLRRIQAEALLNSQKLLQVKNGILVLGFPSEVLRSKMEIPENIEVTR
                     RVIQNLLQVDLPIQCVLVTEKGAISSSNDSEPNGLVDAAINLGGKLIHKD"
     gene            41540..41875
                     /locus_tag="P5516_03105"
     CDS             41540..41875
                     /locus_tag="P5516_03105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HRT91458.1"
                     /translation="MAKGFNRPAGGQGSGGMMAQIKRLQEQMETAQQQLALETVTSSA
                     GGGAVKVTMTGDQRCTEVTIDPDLLKDADAEMLQDLVLSAVNLALDDSRKLAAEKLGP
                     LAGGMPGLF"
     gene            41888..42496
                     /gene="recR"
                     /locus_tag="P5516_03110"
     CDS             41888..42496
                     /gene="recR"
                     /locus_tag="P5516_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HRT91459.1"
                     /translation="MPILPVPIQEMIGALERLPGIGPKSASRLAFFLLRAPQDLVQQL
                     TSALTNLKSATGTCQLCANITLADQELCEVCRDPQRADGTICVVEEPLDVLALERTGG
                     FHGRYHVLQGVLSPIEGVGPDDLTIRGLMERVRAGEAKEVILATNPSMEGDATALYLR
                     QQLLALGVRVTRLARGLPVGGDLEYADQHTLLRALAGRQEMT"
     gene            complement(42543..45023)
                     /locus_tag="P5516_03115"
     CDS             complement(42543..45023)
                     /locus_tag="P5516_03115"
                     /inference="COORDINATES: protein motif:HMM:NF014044.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HRT91460.1"
                     /translation="MMSSDNSNYFIGRVYDPKTKGPTQEDVLLDSANLTTHCVVTGMT
                     GSGKTGLCIDLLEEAALKKTPAIIIDPKGDLTNLVLHFPNQQPADFQPWIDPEAARRE
                     GKSVEQMAEETASRWSKGLAEWGIGKEKLQALAEAAEYTIYSPGSTSGIPVNILSSFE
                     APEIPFNENAEVLRERISTTVTAVLGLIGLSNIDPLRSREHILLSSLIENAWIHDTSL
                     SLTELILQVQNPPMDRLGAFPMESFFPEKNRFELALLLNNFLASPSFQVWQQGQPLDI
                     GALLYTADGGPRHSIFYLAHLSENERMFFVTLLFAAVESWMRGQRGTGNLRAILYFDE
                     IMGYLPPASSPNPNPPSKVVLLRLLKQARAFGVGLVIATQNPLDLDYKALSNAGTWMI
                     GRLQTSLDKQRLMDGLSSASGSTDIRTIDNLISGLEKRVFMLHSVYRSKPVLFNTRWA
                     MNFLAGPMTRDQLAAANALAGVNAHATGAPTETVKPAQKTTSTVREGSGLTSSQPAIP
                     GDIAEYFYPNNLGISEAAATAQFSGALDSEGLAYRPGIIAQAEVRFVSRQYNLEQINK
                     TCAILEDPGSGLVRWDELVSSEVDKDRLEMHPLPKAKFHPVPSWLSDARKRDELKKDF
                     LDWIYRSGGLKILANNKLQLFGTPGIPEAEFRRSVQDAVDKTVEEEIEKASKAADSKI
                     AAMERKIQSQELEVKAAESTVNQRRLETMATGGAVVIGMLTGRKRSISSTLSKNRMAS
                     AAKDKLEAKKTTLAQYTEQLAELKKAKEELVAKVKEKWDGIADEISEITIKPTKSDIF
                     PDIFAVVWLPYYIVEQEGKKIELAGFKR"
     gene            45154..47442
                     /locus_tag="P5516_03120"
     CDS             45154..47442
                     /locus_tag="P5516_03120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91461.1"
                     /translation="MYNLELHSMYLLGGKAQSTLPGLIALSPPRKCARGREFDQLVAH
                     VDLRGKTSITPEAMQEWMLKKGALFFNTPGSTTNAMRMMADSINNELFDRNLKKAADR
                     PQVNGTLCMVVLRRDTIYSLVIGQGRIFILEDEAVIEVADHDNPPGGLGTHQSLNCLF
                     SQRNVSSNSKVLLSPLPSPQWTPAFLAGGGRLPADVLGRRLLNQCPQELRGMLLLLSE
                     GSGKITNSTLIQAVPTDGAVTSSAPAMPDTKPAIQPALIDLREVAKRPAQAEPAGDPH
                     PAMKNTADSRLPAAIKSGLAKGQKTPIQRKNVSARRRRQTGLQIDPEKIKARVGSAVD
                     VVDDVTEKVKRKSSSFYEKLFPSEVDTPRSLLLLLAIVIPLIVVSAAGLVYIRRGRTQ
                     QFNYYYQQGQQYFIQSEAQKEDEIMYLFNLQAAMMYMEKASEFGQTADSIALSSTIQS
                     QLDAIQGVARLEITSLNMNDVLSKVNITQMVATTNDLYLLDGQSGRALRYSLSGNTYI
                     KDDLFDCGPNPDNPLNRIGALVDILPLPAGNSFGATLFGIDAYGNIEFCIPGKSGTIS
                     ALIAPEVGWQGIKAIAMYQNYLYVLDPGNNGVYMYPGAGILFEEKPTLFFDHSIPDLK
                     NTVDMEVNADELYLLRANGEMVECTYSHMKDYKLTECEDPAPYSDMRSGNEPQAITFP
                     DAQFVQMRLTAAPDSSIYLLDTHGSSLFHFSLQRNLQKIIQPLFVDSGYRPKFAASSM
                     AISPSKMVFLAFGNQIFYGPLP"
     gene            complement(47456..48655)
                     /locus_tag="P5516_03125"
     CDS             complement(47456..48655)
                     /locus_tag="P5516_03125"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRT91462.1"
                     /translation="MESSDDSEILEHIVRRVFQIEDITYGTNQQAYRVRYRGHLLQPD
                     SATAYDQLSALLKPYQLTPLFRVEEKKQVIYLVSALPAPKPGRPLVNLLMFLLTLVSV
                     LVSGGLTSFEGELSKQPFTAALELFRAGWPFAVSLLAILAAHEFGHYFAGRAHGVHVT
                     LPFFIPLPFTPLGTMGAFINMKEIPRNRRVLMDIGVAGPIAGYIVSILVILIGLSLSR
                     LDTLPLHFPPGHGVQMEGNSLTYLLLKYIHFGQVLPQPASYGNTAPLLYWLRYFFTGR
                     PFPLGGLDVMIHPVAWAGWAGLLVTSLNLIPAGQLDGGHIFQLLFGAKWSRRILPFIL
                     VGLVGLGFVWSGWWLWAMLVFLFGRVYAEPLDQITQLDPLRKALGFTALVIFLLTFIP
                     VPLSVIA"
     gene            complement(48686..48779)
                     /locus_tag="P5516_03130"
     tRNA            complement(48686..48779)
                     /locus_tag="P5516_03130"
                     /product="tRNA-Sec"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(48743..48745),aa:Sec,seq:tca)
     gene            complement(48846..49391)
                     /locus_tag="P5516_03135"
     CDS             complement(48846..49391)
                     /locus_tag="P5516_03135"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRT91463.1"
                     /translation="MRFERLLQQDIQITERLRLTNPDSRLKPIAIFLAHSGDSWFILL
                     GLLIIWFLSVGLWRSMTALMAAGAVGLALVVLAIKFTIRRRRPEGKWGAIYRNTDPHS
                     FPSGHAARTAMLAVIGLVIGPLWFGLLMLVWAPLVSLARVWMGVHYFSDIVAGILLGI
                     LAGVLVLQLAPWLSATFPYIF"
     gene            complement(49401..>50029)
                     /locus_tag="P5516_03140"
     CDS             complement(49401..>50029)
                     /locus_tag="P5516_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91464.1"
                     /translation="AELVDSVAVIGLDDSCGLHCVKRLTYLPDTGSLLQNIQRGAEHF
                     SQVTPADSHILSVSADVPAVTPEIIDACIRIFNAREFDIYYSVIERSAMEKRFPDSRR
                     TYLKLKDGEVCGGDVNCIRKKAALDPDSAWSGLIERRKNPIKQASMIGWGTLLLLMTG
                     MLTLDNAASRVCQRLGIQGKALKMPYPEIGMDVDKPFQHNIVERDLLR"
BASE COUNT        11893 a        12519 c        14245 g        11272 t
ORIGIN      
        1 gtattcccca cacgcgtggg gatggaccgc cctgatcaaa cagttgactg gcggcgacac
       61 cgtattcccc acacgcgtgg ggatggaccg tgaatatata taactttttc gaggtcactc
      121 ctggagaaaa gcacaggcga aattacgacc agttacccac agcgcggaac caggcttcaa
      181 agcggcgcgc tttccgctcc agcagccatt ccaaaactcc ggggttgccc caaaaaccgc
      241 tggattccat gaattcgaac atgcgataaa aaatgctgac ggcgtattgc ggcatgcccg
      301 tgcttgccgc ccgcgcgcac caatcttcgc gtgaaatcac cttcagagca atctccttac
      361 cggtgacgag ccggatgata tgcaaaactt cagtttgaga aaggatttct ggcccggcga
      421 gctcgtaagt tgcccagtcg taggttttcg gggtgagaag cacccgcgca gccacctcag
      481 cgacatcgtc cagatccacc atgcccagca gagtggaagg ctgatacgga atttcataca
      541 cgcctctttc aaggatgttc tgccgctgcc ccagtacatt ttgcatatat ggcgttggct
      601 ggaagatggt aaagttgagc cccgattcct taatcttttc ttccacgcgt aatttcaacc
      661 aatggttggg tagcgattcg atttgcgggt ggcataaaga gtgaaaaacg aagcgtggaa
      721 ccccagcctt gatcgcggct gccaccgccg tttgaccgaa ttccaattcg cgcgcgtgaa
      781 cgttcgggga gatgtgataa atggcggctg ctccctccgc agcgcctatc atatcctcaa
      841 gcgagcccat gtctccgagg acggcttccg cgcccagggc ggtgatcttt tgagcgttta
      901 tatatgaatt gacaataccg cggacacccg ccccgcgcga tatgagcgcc tggataacag
      961 ccctgccggt tttcccaccc gcgcctgtaa caagaatcat ttcattcctc attgtgtgtg
     1021 aagtataagg ttattatagc atcgagagga gcatccattg tttggatgtg accgcccagg
     1081 gcgcgagact gaattgagcc atgaaaaaaa gcaagtggtt tatactaggg ggaagtgaaa
     1141 gggtatggaa ggaggaaaaa agggatgacg atcatcgaca ggttggcagg gctattagat
     1201 cggattttga tatctggtca agacataggg gatgtattgt tctcggtgtt gttggcggcg
     1261 gtaatggggc tggcgatatc gcggatatac aaatggacac atcgagggtt gaactatgag
     1321 cacacgttta tggcgacgct ggtattgctg gcgccgatcg tgtcgatcgt gatgttgtat
     1381 atacgtggcg atttggtgct gtcgcttggg ctgataggca gcctgtcgat cattcggttc
     1441 aggacgccga tcaaagacac gcgggacatg gtatttttat tttgggtgat cgcggtgggg
     1501 ttgggggcgg ggacctacaa ctgggcggtg gttggggtaa cgaccccgat cgtggcggtg
     1561 atcgttgcgg cgctgtactt tttcaggtat ggacattcgc cgaacactga ttttgtactg
     1621 gtggtgaacg ggagtggaga atacaggaaa gaagagatcg aaaacgtgct ggagaaatat
     1681 acgactggaa agcgggcgag gtcgtttgat gtagatggag agaagtggga agtaatctac
     1741 gagctgaggt tcacgaagag ggagaagcag ccggtagaag ggatggtcgc agagatgaag
     1801 gagatgagcg gagtgaacaa ggtgtcggtg ctggctccgc agctggcgct tcctctgtaa
     1861 agggcagggt gagagaaaac gggaatgaag ccatcagcgc ggttggatga aaagaactgg
     1921 cggttcgagt acaagtatcg gctgtccgta gagcagtatt tgcgggtgag gaatcagatt
     1981 cgggcgtaca tggagataga tgagtacacg gagcgcgcgg cggagaagaa gtacctggtg
     2041 tgcagtgtgt attatgactc gcttggatta gacgcgttta atgaaaagat agatggcaac
     2101 agtgaccgca tcaagtttcg cctccgcacg tattgcaggg agcttagcca ggggacgacc
     2161 atccgggcgg agatgaaagt gcgaaaaaac gccataacgg agaaatatgg cgcgttcatc
     2221 agcggagcgg aatacgagat gttcataggg cagaagcgct ggccgaaaat acgtaaccag
     2281 gtgatggaag aatttgagcg atatgttcat ctgaagggat tgaacccgct gctgctgacc
     2341 gaatacagac gggaaggata tcgatcgagg gcgcgggagg agatacgcat cacctttgat
     2401 caccgggtga agagcgcgca cagccgaacg ttgtttcctg aagcaccgca ttttcacgag
     2461 caccatccgg ggctgatcgt attggagata aagtgcatca aacagcagcc ggggtggttg
     2521 cgcaggttgg tgcagcagaa cgggttgatg atcatggcga acagcaagta cgtgcagggg
     2581 atgatggtgg cgtaccccgc gctggtcacg ccagggtgga gcgcgtaagg gaaaaggaaa
     2641 agaagcggca gagagcggat gagcattttg aagagcaaaa acctcgttcc tgttagtgcc
     2701 acattgatag cgggcttgtg ctttgcctat agcctggtgg tgcgtttctc ccatttctta
     2761 ctggtgtcgc gtggggaaaa ggcgataacg ctgggtgttc attggttatg gatcgtgttt
     2821 gcggggctgc tgtttatact tccgctggtg aacaggttat tggaggggat aagcaaagag
     2881 aggctgaaaa taatcggggg gctaagcctg gtgacagcgg tggtcatcat ggcggtgttc
     2941 tacagccttc cggcctttcc aaagcgaatg gagctggaga tcagggcgaa aggagaggag
     3001 ctggcagtgg cgggaaaggg gatcaccatc ctgtcgatgg tcaggcagga cccgccgaag
     3061 aacattccta ccccaatcaa tcttagaaat ctgagctacg ggggtggttg gcaggtgatg
     3121 gaagggaaac tgaggttctc ccccgggcag ggggacagcg gctcggtgcg ctacacgagc
     3181 aatattcagg gggaggtcag gatacgtttt cagaccgggc cgggagaagg ggcggtggat
     3241 atcctggtca atggggaggg gcagagcgtt gagctggctg aggcggagga gggggaggtt
     3301 gagaaaacgg taacgatccc gaacacgtgg aagaacgcgg accgggtacg gcgggtgttg
     3361 ctgatggcgg gactgctgac agatacggtc acggtatttt tgtccgcggt gatgttttgg
     3421 ttgctggtat accaggtagt ggttaaacgg cgtctgcggg cgcgggggct ggctgatgtg
     3481 gtgggtttgg tgcttgtttc gttggtgttg ttgtttgcca accagcaggc acaacgcgaa
     3541 attgtgttca gcgatccgaa acttgaggag ggggtgaggg aagctttagg gaagccggaa
     3601 gggcggctat acacgcatca actcttgacg ctgatcgagt tggatctgag cggtagagga
     3661 attcgggagc tggaagggat cgaggggatc aggaacctgc gggtgctgga tttgagtgat
     3721 aatcaaatcg agaacctcac tccgctgcaa ggattgaaga agttgcagaa actgaacctg
     3781 agagaaaacc gggtaagaga catcactgcg ctggctgaaa tgaagcagct cacctaccta
     3841 aatttgcaca gcaacgagga tatccaggac ctcacgccgc tcaagaacct gaccatgctc
     3901 aacagtctca ttttgagaaa tgtgcccata ggagaccagg tttcgatatt gactaacctg
     3961 aacaggctga cgtatttgaa cattcggcac acatccatac ggaatattga ggtactggta
     4021 gaaatgatga gccatggggt cttgcaggat gatgtgagcg ctggtaaaag ggtcacggtg
     4081 aacatactgg aaaatgaaat aggggaagga aaaggggacc cgttcttggg gctgaggggg
     4141 tattgggaaa aggtgagcta tcggtatcct tactacttac ccaattacgc gagtgaagtg
     4201 gcgggtccaa aattttcaca tgagagtgga ttttacgagg aggggtttgc gctggtactg
     4261 acaacgccag aagcaggcgg gaagatattt tactcgaccg atgggtcgga accggcgatc
     4321 gatgaaaaac ggatggcaat ggggagtacg cgggaataca cggggccgat aacggtaaat
     4381 gacaagagcg cggggcgcaa ccgtctgtcg caaattgaaa cgagtagaat agacgactac
     4441 aaaccaccgg aaaacatgtt caaagcgatg gtggtgcggg cggtggtagt aggcgaagac
     4501 cagcgaagga gcggggtggt cacgcacaca tacttcgtgg aggagggggt gaaagagcgg
     4561 ttctcgctgc cggtgctcag cctgacggtc gcgccggtga cgttgtttga cgaccggtac
     4621 gggatctacg ttccgggcga gctttatcaa aatgaccacc cgtcaaatca attgcataat
     4681 accgcgaact acacgcagag gggtttgaaa tgggaaaggc cggcattcgt tgaactgttc
     4741 gatccgtcag gagaggtggt ctttgcgcaa gagattggta tcaggattca cggccgcgcc
     4801 tcgcgagggt acccgcaaaa atcattgagg ttgtacgccc gtgaagagta cagcggtgaa
     4861 agcaccctgc agtttgattt ctttcctggc ttgaacgagc gtttaaatcg gctggaagtg
     4921 gataagtttg agacgctgat cctgcgcacc gggggagcgg gagacatgga agataccatg
     4981 atcgcggaca tcatcggaca gtcgctgctg gagaaccttc agctggatat acaggggaac
     5041 tacccggtca tcgtgtttct gaatggtgaa tactggggca ttcataacct ccgcagccgg
     5101 tatgacgcgg gatatttcca gaattattat cacataccgc ctggggatgt ggtggtgctt
     5161 gtaggcagtg aatcagaatt atatctgggg gacccgggtg atgagaacct gtacacggat
     5221 atgctcaggc tgatagacga gcattatgac acgaacggct ttcgcaccgt cagcacgctc
     5281 gctgacgcgc aggcttacgc ggcgatgagc gagctgatgg atatggagaa ctacattgac
     5341 tataacatat cacaaattta tcccaacaaa gtggattggc ttggaaacaa tatttggtac
     5401 tggaggaaaa aagtagattg cgcattaccc gagagcggcg agatgtatgg gcacgacggg
     5461 aaatggcgct ggatggtcat cgatatggac tcgggcttta aagaccgata cttcaactca
     5521 atggagaacg tgaccgcaga aggttctcag ggaacttatt tgttccgttc gttgctgcaa
     5581 aacgaggagt tcagaaggtc attcatcaat cggcttgcag acctgttgaa cacgaatttc
     5641 aagacagagg ttgtgttggc gacgatcgat gaagtgcagc gggtatatga accggagatg
     5701 gaagagcata ttttgcggtg ggggatgcca gggggatcgc tggcgggctg gcgggcaaac
     5761 gtggaggaga tcagagaatt cgccagggcg cgacccgaag aactccggca gcagatagcg
     5821 gattattttg atttggcggg gagcgcggaa ctgagaatca gaacagaccc gggcaagggg
     5881 tacgtcacgg tcaacaggct ggaaatccga gaaggcatgg ttggaattga agacccttct
     5941 aactgggcgg gagtctattt ccagggggtg ccgtttacga tcaccgctgt gccggctgaa
     6001 ggctatcgtt tctctcactg ggaggggttg aaggggggag actcaacagc gcagagtcag
     6061 gagatcacgc tgacgggaga cctggcgctg acggcggtgt ttgtgcgtta attgccacgc
     6121 ccttcttctc cagacccatt ttttccaacg ctgcggtaga tggcagaaag gcaggctcaa
     6181 taaatacaga aaaaacgtct tatttcttcc atgtcagact ggtgccccac tgttttgatt
     6241 ggtggacagg attgattaag cgaagtttcc tgaaattcat gacttatatc ctattcgcct
     6301 tgattcctga tgcataattg gttaccatta aggagtcaaa gacccgcaga ggtggttatt
     6361 tcccaaacca cccgactgat tatttattgt gaaaggagca aggaatgaaa tttaaatacc
     6421 tgggaaagag ttacaaccga ccggacgcga tcagcaaggt gaccggcaaa gcggtttacc
     6481 tggatgatat tcgcatcccg ggcatgctgc acgcggccat cctgcgccct gagtacgcac
     6541 acgccgaaat tgtccgtatt gataagactg aagcggaaaa gatgccgggt gtggtgaaag
     6601 tggtcacagg agaaaactgc cactatcact atggggataa tttcaaagac ctggtgccta
     6661 tggcggtgaa gaaggtgcgc tacatcggtg agccggtagc ggcggtgatc gctgaaacgt
     6721 tggcgcaggc tgaggcggcg ttggaaaaga tcaaggtgga gtacaaacct ttgccagtgt
     6781 atattgatgc gcgtgacgcg atgaaacccg gcgcagcgct tgttcatgaa gaaaacggcg
     6841 agtactggca cctgcccacg ctaaagccca cacctggcac caacatcgcc catacctatc
     6901 accttaagaa aggcaaaggg gtcgatggtt tcaaagacgc cgatgtcatt gtcgagggtg
     6961 aattcaatta cccgctgggc tcatgcgcgg ctatcgaacc gcatggttcg attgtgtggt
     7021 tcaaagagga taagaccatt gaagtctggt cttcctccat ttgccccttc atcattcgtg
     7081 atgatctggc gcactcttat ggtgttccag tttcagatgt gcgcgtgcgc atccccgata
     7141 ttggcggctg ttttggatat aaatctgata tcactgttga gcaaaccgtc gcctggattg
     7201 ccagctttgt gccaggtcat ccggttaaat gggtggcttc acgcaaggaa gatttcacct
     7261 ccacgctcgt ggctcatggt attcgttcgc gcatgaagat tggcgcgcgt aaagatggca
     7321 agcttgtggc catccaaaac gaggtgctgc acacgggcgg cgcgtacgcg gatacggcgg
     7381 tcaatatcac tatcgctgcc acgcacaact cagccggacc atacgaattc gagcactgcg
     7441 acctgacggg ttacacggtt tacaccaata ccccgcctgt tggcgcttac cgtggttatg
     7501 gtcaccagga agcccagatt gccaccgagc gcatgatgga cctgctggcg cgcaagctga
     7561 acatggaccc gatcaagctg cgagagatga actatcttgt cgaaggcaag gtaaatgcgc
     7621 tgggcgaaaa gatgtggaag agccacggag acctgatgac ctgttccaac atggtcaaag
     7681 aaaaggtctt ctccaaaccg aaaccagccg aagatgaaaa ttattactac ggccgcgggt
     7741 tcgcggcggt gatgaagagc cccaagggcg cgccgttttc cacaaaaagc tgttatatga
     7801 agatgaatat cgatggttcg atcagtgtga gcatgggcgg cgctgaagta gggcaggggc
     7861 tgcgcactgt cgttcaacag gtgacagccg aagcattgaa gatcgccccg gagcgggtga
     7921 gggtgtacac cgagatcgat acgcagtatt caccgtatga atggcagacc attggctcaa
     7981 tgttcaccac ccagggtggg cgcgctatcg tgcgcgcggc ggacaagctg atcgcggtgt
     8041 tgaagaacac tgcggcacag gtactcaaga cagatgtgga ttacctggaa tatgatggtg
     8101 attacgttta cctgcgcaat gacccggata tccgtgtggc ggtggcagac ctaagccgcg
     8161 gctatattac ttcagatggc atcaccattg gcgaatatgc gcacgccgtt tctgacgcgc
     8221 gcctgccgcg ctacgccaac ccagacgaaa acgggcaggg cagcctgggt gtgaattaca
     8281 ccttcggcgc tacggcagcg gaaatccgta ttgagaagaa aactggcaag atcattgtgg
     8341 ataagttcac ctcggtgttc gacgttggtc aaatcatcaa ccccaagcag atccgcggtt
     8401 cggtcatggg cggggtgacc atggcggtag gggcggcctt atacgaaaaa ctggacatca
     8461 gcgaagacgg taagatcaac aacccgcagt tgttcaagta ccatattccc acttacaaag
     8521 aagccatccc gatggatatc gaattcatcg aaacgcccga tgccgttggc ccgtttggcg
     8581 cgcgcggggt tggcgaacac tcggtcattg gaccggcgcc ggctatcatg aacgccatcc
     8641 gtgacgcggt gggtattgat ttctttgaga ttcccatcac accggatgtg atgaaaaaag
     8701 ccctggaagc caggcaggag gtgaaataat ggcagaaaaa atcactttta ctattaatgg
     8761 gaaattgacc aaggttgaag ttgaccctga gatgctggtc attgatgtga tccgagatgt
     8821 gttgaacttg accggcacca agcgtggttg cgataattcg gtttgcggca cctgcaccat
     8881 tgtggtgaat ggcaaggcta ccaaaagctg caatatgccg atcaagaagc tcgctggcgc
     8941 ggacgtgctc accgtggaag ggttggccaa gggcactgag ttgcatccta ttcagaaggc
     9001 aatttccgaa tccggcgcca ttcaatgcgg cttttgcacg cccggcattg taatggaact
     9061 gtacgggttg ttggaagaaa aaccagatgc cgctcagagt gaaatcgaga acgcgctcag
     9121 caagcacctt tgccgctgca ccgggtacga acccatccgt gacggcgcgt tgatggcgca
     9181 gaagctgatc aaagagagaa agtaaccctg cttacacact cagcgaccct tcttttagaa
     9241 gggtcgttgt gttttatcca aacctgccgg taatataatc ggacgtgcgc tgatctttgg
     9301 gcacctggaa gatttcgatg ccgctgccat attcaatcag ctcacccatc aagaagaagg
     9361 cggcgtaatc ggccaggcgc gcggcctgct gcacgctgtg cggggcgatg aggatggtgt
     9421 actccttttt taattcccgc agcatgtctt ctatggatga ggtgacgatc ggatcaagcg
     9481 cggaagttgg ttcgtccagc aggatgactt caggttcaag cgcgagcaca cgtgccaggc
     9541 acatccgctg ctgttgaccg ccggaaaggc tgaaggcgga ttggttcagg cggtcgtgca
     9601 cttcgttcca caggaatgcc tgtttcaagg ctttttctac tttttcgccc agcctggctt
     9661 gattgtgttc gccagccatc accagcccat acgccacgtt atcaaagatg gacaaaggca
     9721 ggggaaccgg gcgagaaaaa accataccga tttttcgccg caaggtgatg acatcctcct
     9781 cagggtcaag gatgttgcgc tggtgaaaaa gcacccggcc ttttatctcc ttcacatccg
     9841 ccaggtcatt cagacggttc aaggtgcgca ggaaggtgga tttaccgccg ccagcaggac
     9901 cgaagagcac ggtgatggcc cgtgattcga tttccatcga aacgttcttc aggctctcat
     9961 tattatctga atatcgcact gaaaggtttt gtacctgtaa cgctttcatc gggtcaccat
    10021 tggcgttttg cccgcgcgcg agaacggatc aaggtggcaa tgaggttcat tcccagcacg
    10081 aacaccagca acaccagcac ggttccgtat tggatctgga tgggcatgcc gggaatctgg
    10141 gtggagatta cgaaaatgtg atacggcagc gccatggtgg cgtctaacgg cgagcgcggt
    10201 agttgcggta agaagaaggt ggcgccagtg aaaagaattg gcgcggtttc accggcagag
    10261 cgttccagcc caagaatgat gccggtgatg atcccgggca aggcttgcgg cagcgtaata
    10321 cggcggatgg tctgccagcg cgttgcgccc agcgaggtgg agaccacccg gaatgagttg
    10381 ggcacgctgc gcagcgctcc ttcagtggtg ctgatgatga ccggcagtgt catgatggaa
    10441 agggtcaatg acgcagccag gatgctggtg cccaaatgaa ggaacaacac gaataatccc
    10501 aacccgaaga gcccgtaaac cacggatggg atgcccgcca ggttgatgat ggcgatgcgg
    10561 atgatgcggg tgaacgctgt ctcctgggcg tattctgcca ggtagatgcc tgccagcaca
    10621 ccgatgggca ctgaaaagag cgcagtaccc agcgtgagat aaagcgtacc cacgatggcg
    10681 ggaaaaatcc ctccttcccg cataccgttc gacggcgcag ctgtgaggaa ttcccagctg
    10741 atggctggcg cacccttgac cactatataa accaccagtc cgatgatggg aatgatggtg
    10801 atgagcgtga ccagccgcag caggtggaac ccgatggttt gcgccatctt cgcgcgggcg
    10861 aggtgggctg ctttcatcag gataacaacc tctcagagcg ttgacgggct ttggtgctta
    10921 ccaggctggc ggtgatattg ataacgagcg agagaaggaa caaagtgata ccgataaaga
    10981 acaacacctg gtaatgaagg ctgcctgaag caacttcacc catttctgaa gcgatggtgg
    11041 cggtcatggt gcgcaccggc ctgaaaagcg agtttaaatg caagggcatg agcggagcgt
    11101 taccggtgac catcatcacc gccatggtct cacccagcga gcgcccgaca cccagcatca
    11161 cagcggttaa cacgccggtt ttcgccgcgg gcagagtcac atcccagatg gtttgccact
    11221 gcgtggctcc gagcgccagg gatgcctggc ggtaggaatg gggtacagtg ttgagcgcgt
    11281 cttcggccac agagacgatg gttggaatgg aaataaatgc cagcaaaatg gcccctgtga
    11341 acgcggtcag cccggtggga atattcagca tttttcgaaa caaaggcgca aaaaccaaga
    11401 tcccgatgaa cccgagcacc accgagggga tgccggcaag gatttcaacg atgggcttta
    11461 aaaggtcgcg cagccagggc ggagcgactt ctgagagata tactgcggtg ccaatgccaa
    11521 agggcaacgc gataagggtg gaggcgaggg tgatcagcag gctgccgaag attaacggca
    11581 ggattccgta gtaaccttca atcgggtacc aacgaatgct gaagagagag cggagcggga
    11641 tattggttaa tgccggtaaa ccttcacgca gcaagaagaa aagaatgagc accacgaaca
    11701 ggatgctgga atacccgctg atacggatca caccgttgat gaccaggccg tctttttctt
    11761 ctcgtgcctt catgataggg ttctcattcg tttaaaaccg gcatgaaccc aagtttgtgc
    11821 gcgatctcct gcgcttcagg gcccatgatc caatccagat aggttttcac caacccctca
    11881 ggttcgtcaa gggtgtacat ataaaggttt ctcgaaagga ggtacgtatg gttgctgaca
    11941 gtctccagag aagggaggat gaagttcatc ggctcttcaa gtgaggatat tcccaccagt
    12001 ttgacatcgc tggtgatgta acccaggctg tcgtacccga tggcattagg gttatctctc
    12061 acctcagcga taataccttc agaagagggg agcagcaagg tgctcgcaga aaaaatggca
    12121 ttgttatcca tctcgcccat gcgcaccact gattccagga agaagagatg tgagccagag
    12181 ttggtttcgc gcgaaaggcg gacgatttcg cgatcttctc cgcccagttc gctccagttg
    12241 ttgatctctc cgcggtagat acgagaaagc tgctgcaagg tgagattgct gacgaggttg
    12301 cgagggttga cgatgatggc gatggcgtca cgggcaatga tgaattcctg gggatcgaaa
    12361 ccagcctgac gcgccgctgc cagttcttct tctgtaatcc ggcgtgaggc gttggcgata
    12421 tccaccgtgc cgctgataag cgaggtgatg ccggtgccgg agccacctcc gctgaccgag
    12481 atgcgcgcgt tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12541 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ncagcgtaaa
    12601 aattgaatga ggtataatca tgcggctgat aacacaggaa aggttgaatt atggatttcg
    12661 gaacagtaca ccaggtggat attgacgagc aaatgcgctc cgcttacctc gattacgcga
    12721 tgagcgtgat tatctcacgc gcgctgccgg atgcccggga cggactgaaa ccggtgcacc
    12781 gccgcatcct cttcgccatg gcagatatgg ggatccgttc caacacagcg tacaagaaat
    12841 ctgcgcgtat tgtgggtgaa gtgttgggca agtatcaccc gcatggagat tcagccgtgt
    12901 atgacgctat ggcgcgcatg gcgcaggact tttccatgcg ctacccgttg gtggatggac
    12961 agggcaactt tggttccata gacggggatg cgcccgcagc catgcgttac actgaagccc
    13021 gcctgcacgc gcatgccgaa gaaattctca ccgatattga taaagacacg gtcgatttca
    13081 cagacaactt cgatggttct ctgaaagaac cagttgtgtt gccggcgcgg ctgcccaacc
    13141 tgctgctgaa cggctcgagc ggtatcgcgg tgggcatggc taccagcatc cctccgcaca
    13201 acctgcgcga actggccaat gccaccaatt acctcattga ccattatgag aagatcgatg
    13261 aggtttcggt agaagaactg atgcgttttg tgcagggacc ggatttcccc accggcggca
    13321 ttattgtcgg tcgagacagc gtacaccagg cttacagcac cggacggggc aggctggtgg
    13381 tgcgcggtca ggcgcacatt gaggagatga aaggaaaccg ctatcagatc gtcatcaccg
    13441 agattccgta ccaggtgaac aagaccagcc tgatcgaacg cattgccgag ctggcgcgcg
    13501 gcggcaggct ggacgccatc acagacctgc gtgatgaatc tgacaggcgc ggcatgagca
    13561 tcgtgttgga gctgcggcgc ggagcgcagc caaagcaggt gctgaatcaa ctgtataaat
    13621 ataccccgct gcaatccacg ttttctgtgc atctattagc gttggtgaat ggtgagccgc
    13681 ggctgctttc cttaaaacgg gcgctccaaa tctatattga gcatcgccag gtgatcatta
    13741 cccggcgttc gagttttgag ctcgagaaag cgaaaaaccg cgcgcatata ctggatggtt
    13801 tattgatcgc cctggctaac ctggatgatg tgattaaaac catccgcgaa tcaaaagatg
    13861 ctgaaatcgc caaagaacgg ctgatgtcga aatttaattt aacggatatt caggcgcagg
    13921 cgattctgga tatgcaactg cggaggttat ccgcgctgga gcggcaaaag atcgaggatg
    13981 agcaccgtga gattatggcg cgcatcgaat atctgcaaga cctgttggcg catccccaga
    14041 aaatgctggg tttaataaaa gaagatatga acgccgtttc agaaaaatat ggtgacgaac
    14101 ggcgcaccaa gatcgttggg gaaactcacg aagagctgaa agaagaggat ctcattaccg
    14161 atgaacctgt gctgattaca ttcacgcgta aaggctatat caagcgcgtg gcagcaaggc
    14221 tctaccgctc tcaaggcagg ggtgggcgcg gtgtttctgg gcagaccatc cgcgaggaag
    14281 atgaaattga gttcctcatc ccggcgcgaa cgctgcacac cgtcctcttt ttctccgacc
    14341 gcggcaaggt gtattctgaa aaggtgtatc agattcctga tgccaaccgc acggaccgcg
    14401 gcatccccat tgtgaacgtt ctctccctgg acaccggcga acgcatcacc gcggcagttt
    14461 ccgtgcctgg ctttgaggaa ggttcatact ttacaatggc gaccctcgct ggcagggtca
    14521 agcgagtggc gctggcggat ttctccagcg tcagaccttc aggactgatc gcaatatccc
    14581 ttgacgaggg agatgagctg ggctgggtgc gcctgaccag cggtaaagat gagatcatca
    14641 tggttacccg cgccggtcag gcgctgcgta tacctgaaac cgaaatacgt tgcatgggcc
    14701 ggcctgctgc cggtgtaacc ggtatcaagt tgaaaactgg agatcgatta acctcgatgg
    14761 acgtgatcga accagaaggg cagctgctta ccgtgaccga gcatggtttc ggcaaacgct
    14821 ctgacctgga tgaatacccg gtcaaaggac gggcgactgg cggtgtggcg accatcgccc
    14881 aaaagcaaac ggcgagggtg gggcgaatca cctcggctcg cgtggtgcag gaggaggatg
    14941 aagtgacgct catttcttcc ggcggcgtca tcttgcggtt gaaagtgaag gatatttcaa
    15001 tgagtggcag ggcgacgcgc gggttcaagt tgatggacct gggagacgag gatgttgtcg
    15061 cttcggtggc gcgaatccaa acttcggata tcactaaaac cgaaggtgac tcgctgccgc
    15121 ttgaaaacgg gaaagcagag acgcctggtg aagaacctgc cggagtgtag ctaatccctt
    15181 cgcctgagga atgaacgaag gcaatcaacc ccttcccggg tagagagccg ggacggggga
    15241 tgagctcgtg cggcgcatta aattggcggc atgatttttt gaaacacgac gagcagcaag
    15301 atgccgagga tagcgaccat cagaaagaac accaacgcga cgatgaaacg ttgaaaaggg
    15361 ctcatgccca aaaagggcgg tcgctcaatg gtgtatcttc caccccggat atgctcttct
    15421 tcaattaact cttcttcata agcgctgttc acagaattgc gcaggtcatc caacattatt
    15481 ccctcccggg tcatccggtt gtacgggacg caggtgaatg tctccgacag tcacaggcac
    15541 catttctcca ctgtcattga tcagcaacaa ctgacccaat tcgttgatcc ctttaatttt
    15601 accaataatc aagggtcttt ccgaattttc gatgcgcacc tgttgacctt taaaagcgag
    15661 gtgctcttcc cagtattgca tgaatacggg tgtgcccagt ttaccgcgcc aggcgtggat
    15721 ggaaaataac aattcccgca ggatggcagc gcggtcgacg gggctgccgt attccgcttc
    15781 gaggctggtg gctggaaacc ccctcaactc cggtggaggg atcgaacccc ggttgatatt
    15841 cataccgatg cccattatca ccgccgcggg ttgagagccc tgccaggagg actccactag
    15901 gatgccgcaa aatttttgac ccttgagcag cacatcattc ggccatttaa tctgcgggga
    15961 aagcccgcaa acccgttgca ctgtttcgtg cacggcgatg ccgcataggg gcgcgaacaa
    16021 cgccaaccga gggacttcat ctggcgttgg gcgcaagatc aggctgagag ccagagaggc
    16081 gccaggggaa gtgatccagc ggcgatcgaa acgtccgcga ccgctggttt gctgttcggc
    16141 gagcaccagc gcgaagtctg gagcgccgct ctccgcccag cactgggctt cggtgttggt
    16201 tgacccgatg gattcaaagt gatgccaggc gctaaccgct gtcccgcgga gggtttctgc
    16261 tatctcgtgc ctttccatgg ggctaattct actatgtaat acaaggagag cgggattgac
    16321 cgctctcctt gtatatctcc tcggtgacag ggcgttttac ttagcgttga ttttgatggt
    16381 cttcggtttg gcttcttcag ctttgggcag gtgaatggtg agcacaccat ccttcatgcc
    16441 cgcgctgatc ttctcgctga cgatcgggtc gttgatttcg acgctgcggc tgaacttccc
    16501 ggcgggcatc tctgaaaaga gatactctga tttctcatcg cggatttctt gatattcacc
    16561 atcgatcgag agcgtgccat tgttgaactg gatgttgatc tcatcggttg aaagacctgg
    16621 caacaaggcg gtgagggtgt actcgttctc attgactttc atttccagcg ggaaagacag
    16681 aacgcgttcc tggttgaagg tgttctccag cattctcctc atcatcctgc ggcggctttc
    16741 ggctatcatt tctggggtgc gaatatatag ggtcatttca ttcctccata tctttttcat
    16801 aaatttacac acattttagg cgccggatat aagagggtga taaacagatt attaaaagcg
    16861 tattaatatt catttcttct ttgaggtttc ctagctgtgg tatcattccg ccaggaagga
    16921 agacatggaa gataagatca ccatcatcga aggaccgcca ccggtctttg aaaaaattga
    16981 tgacggctgg gcgctgggtc tcaccgaaag ctcggcctat tatgggatgg cgctgacgcg
    17041 gctgcgcact ttcaacggac cggcgctggt ggaacgctgc caccgcaggt ggaaccggca
    17101 ggaaccgatc ttcctgcatt accgcaacga gatggggttg gagcagcagg tgcccatctt
    17161 ggcggcgcga gccagcgagg tggatgaagg gcaggtgctg ttcctgtgga ttcgctaccc
    17221 aatggattcg catggtgacg aatgaagcga tgagttgtaa caaaaccaag cccggcatca
    17281 cgccgggatt ttgttcgtta aaaacaagaa acatctgggt gaggggggca cccaaatgtt
    17341 tcttaccctt aatatacacc agtccgtatt cgatggcaag gggtaaaatg aggtttgaat
    17401 cttaaaattt gtgccccggg agggactcga actctcaacc taagcgttag gagtgctttg
    17461 ctctgtccta ttgagctacc ggggcgacaa tgtgattata tcattgagaa aacagctctt
    17521 caagagaaag agagggatct ttctgttatg agcctatcaa aagaaaacac ccagtttgaa
    17581 ctggaaatac ttgacttcgc ctatggcggg aacgcttttt cccgcctgcc tgatggcaga
    17641 ccggtctttg tgcctttcac catgcctggc gagcgggtgc gagtgcgcgt gacgacggca
    17701 tttaaggatt attgcaccgc tcaactggag gaagtgctta agcctgcgcc cgggcgtgtc
    17761 gctccccgct gccagcattt taccgtctgc ggcggatgcc attaccagca ccttccctac
    17821 cagcaacagc ttgaggtcaa acagaggatc gtggcagacc agctgcggcg tatcggtaag
    17881 atcaccgcac cagcggttaa ccagatcatc ccttccgccg atgagtggca ataccgcagc
    17941 accatgcagt tccatatttc gaagaacggc aggcctggtt tccgcgccgg tgatggcaag
    18001 actgtgatcg aggtgaacga atgttatttg cccattaagg gtattgataa actttggccg
    18061 aggttggata tcgacgcgca atcaggcatc cgtcgtgtaa tgctgcgcgc cggttcagat
    18121 agcgagctgt tggcagccct ggaaggcgaa agcaacctgc cgcctgaatt caacctcgat
    18181 ttcccactct ccgtgcatta cttgagcagc gatcgagatt acctgttggc cggagacgcg
    18241 cacaacctga tgacagtgaa tggggtgaac ttcatggttt cgccgcgttc tttcttccag
    18301 gtgaacctgg ggcaggcttg caagatggtg aattatgtgc ttgccaatgt tgacctcaat
    18361 tctgcggtta gcgctcttga cctctacagc ggggtcggcc tcttctctgc ctttctcgcc
    18421 ccacgggtaa aagaactcat cgccgttgaa tcctctgcaa gtgcctgtga cgatttcgcc
    18481 gccaacctcg acttttacga caacgtctca ctttatgtgg gtggcgcaga gcaggttttg
    18541 cctgggctgc gaatagcgcc tgacctggtg attgtggacc caccacgcgc tggcttgatg
    18601 tttaaggtag tggattacct ggtcaacagt ccggtcacgc agctggtgta cgtctcctgc
    18661 gacccggcaa cgctggcgcg tgattgctgg cgcctgacgc aaggcggctt caccatccgc
    18721 tccattcagc catttgactt tttcccgcag acctatcacg ttgagtgcgt ggtcttcctt
    18781 gataaaaaga gaggatgaag aggtggctgt tttccgtacg gtagagtgtt ttttgaacga
    18841 cgtctctctg ttccctggtg ttcagcggta cagcgaaaag accgccacac gtacgggcgg
    18901 cggttgcagc gactttgaca gggtttcaaa aaaatgtgtg ataattgcca cgaaaaggac
    18961 tggtttgact tgattaccag gcgtctttct acaggagcac aaaatgaaaa aagtgattaa
    19021 gttgactact gctttcctcg tgttaagttt tctgctttct tcatgcaacc tgcctttctt
    19081 ccgcggagac gcggcagaag aaaacaaccc gccgaacctg accctgaccg cgttatttga
    19141 taccagcatg aacatcccgc catcaatgac gccggcgcta ccgacctctg tggaagttat
    19201 tcaaacgact ccggctatag ttcttcccac gctggcaggc agtgattccc ccactcccaa
    19261 tcccattcct accaatactt ccgcgccgcc caccgccgag gtgcgccagc gcagcggtaa
    19321 gttgatgaaa gccgcgtacc tctcaacccc tccggtgatg gacggaactt acgcggaatg
    19381 ggtggacaag actaataaat acgccatccc ctacgtggtg tggcagccct cgaactggaa
    19441 gaacatggaa gacctcgaag gcgcctttgc cgcggggtgg gatgacaagt acctgtacat
    19501 ttctgtaaaa gttacggatg atgtattcgc gcagaataaa agcggagaat ccctctacct
    19561 gggtgattca gtggaggtga tgatcgacgc aaacctggat ggcgattttt atgtgaatta
    19621 cctcaatgac gatgatttcc agtttggcat ttcgcctggc aacccggcga agggtatcga
    19681 ccctgaagtg tacttgtggt acccttcggc actcaagggc agtaaatcgc aggtgctggt
    19741 gtcttcgcaa ttcgaaacca gccccatcta ccgggttgag gtgggtattc cctggtccat
    19801 gctgggcgta agcccctaca acggtttaca cctgggtttt gttgtgtcgg tgaatgataa
    19861 cgataaaccc tcggaaaatc ttcagcaatc gatgatttcc accgcgcaac atcggaattt
    19921 ccttgatccc accacctggg gagaactgat gctcgtcaag taaacgggcg aataaaaaac
    19981 tggctgccga caggcagcca gttttaaaag aggatagagg ccagttcagc ccggtacttg
    20041 cgtgtgagct cggcttcagg gtcaagcagt tccagcaacg cgagcagtac gctgcgggcg
    20101 aggtccttac gatagcgctt atcctgacgt atcacttcga gcaggccatc aatgccggcg
    20161 agggtatttc tgcgcttgac caggcgtatt gagttgacaa agaccgcgtc gaggtcattc
    20221 tcctgcggca gctggtcatc atcaatgtcc tgcagcgctt ttatcagcgg cacaagtttt
    20281 tcggcggcgt tgaattcagg gctggcgggg aaattttgat agagcgagcg cgcggcggcg
    20341 ctgttaccct ccagcagatt gattttaatc atccctaaaa ggcttgggga gtggttttca
    20401 tccaaatcca gcgcctgcat aaaggcttcc atggcttctt tcagctcgcc agcgtgaagc
    20461 aggctgttgc ctttttgcgc cagcaggtca gatggccgag gtggaaggat gcgggcaaga
    20521 aactcgcgca ccacatgctc ggggaccaga ccggtaaagt cagaaacctt gcgcccctgg
    20581 ctgaatgcca taacagtggg gattgagcgc acactgaagc gcagcgccaa gttggggttg
    20641 gcgtctacgt ctaccttggc caacctgaac tctccctgtg actgctcagc gaaacgctcg
    20701 agcatggggg tgagcacccg gcagggcttg caccaatccg cccagaaatc caccacaacc
    20761 gggatgttct gcgagtaggc gatgacatcg tactcgaaac tggattcagt tacggatatg
    20821 atgtgctcag aggtcatgcc atctccatgg tgtcgtatac aaatcctctc cctctgcgcc
    20881 ttactccgaa gaaggagctg aaggatattc gagtttgagg atatttccgc gaatatccag
    20941 taccacgcga aaccccatga gccccaggta tgccctggtt tcttcaggga tgccgtgatc
    21001 caacaagaat tcgtattgct tgtcgatatt ctttaaagat gcttcaagga tggcgtgaga
    21061 aaaaatgtca tcctgcccgg tcattttcgc tgcctgcgca gcgatgatat cttcgttgcc
    21121 ttcgacctgt ttgaataaag agcgcaggat ttccaggtcg atgggtcggg atggcacgtg
    21181 gcgcttaaac ccggcatcat caatgacgta gcaaggctcg ctccctacaa tcgcggtgtc
    21241 gacggggtga tcaaattcat cgctaatcaa tcctttgaac aatggttcag cagccatcat
    21301 catctccacc ctaattataa agcatagtga tgacgtggaa caccggtgag caatagaaga
    21361 gcggctgagg agttttttaa atgctgattt cgacccgagt accgccaact ttactggcaa
    21421 gctgttcttc ataaggcaca acgggcaagg tccagcggta aagccacctg gcggcttctg
    21481 aaaacagatt gatacagcca tggctgcgcg ggcgcccaaa atcgttatgc cagtaagtgc
    21541 cgtgaaacgc cacaccttct tcagtgaagt aacaaatcca gggtacacct ggcaggtcat
    21601 acccccatga agaagccagg ctgccgttga ccatgtgcct tgagggacgt ttgtaatccg
    21661 tgcggaagct accggtgggc gtgaggtagc cattgcgata ttcggtgcca ctcgaacagc
    21721 gtaacatctg cacgggtgca tctccttcgt aagccactac aatctgttct ttcaagcgca
    21781 cttccaggcg cttatcctga gcgttcacct caggagatat gggttccacc tcagccggcg
    21841 cgataacacg caggtgggcg gggttgacat aatatttgaa ccggtaaaag tcttcctgca
    21901 cctcgtacca gtcatcccct ctggcgtctt gcagaatgcc ggtgatccaa tgcgtggaag
    21961 agtaatacag ccgataggcc acgatatttt ttagcagcgg gttccagatg gcgtcggtga
    22021 agggcacggt cacctccccc agcaaacctt tcacggggat gtcttgccgg gtttcattca
    22081 gactgatctt taccggctgc accgacccgg aatgcacgta tccctgcccg tcaagctgat
    22141 accacacgcg gttgtgactg ggctcagagg agctgacttc catgcgggtg atgggcagca
    22201 ccaggtcttt ccacagggtt ttcaagggtt taccttctgc agaaggggtg gggaacatat
    22261 ccaccttgtt ggtggtgatg cggccaagct gggcagccgt gtcttgcagg ctgcgagttt
    22321 gcgcgtaggc gtaagtcgtt gaatggatgg ccagcaagga aaggatggag gcgctgctga
    22381 gcttgaggaa ctcgcgcctg ctcatgttgc gcaggtctaa tgcttctgcc atgtcaataa
    22441 tgcacgctga ccaaagtacc aacgtcggcg aaatcgtata gccaggcggc gtcgctggtg
    22501 cgcatgttca cgcagccatg gctcatgggg gtgccgaagt tgttatgcca gtatgtaccg
    22561 tgaatgccgt agcctctata gaaatacatc gtgtaaggca catccggcag gtagtaatca
    22621 tctccccgca tatccgtgta acggtatttc acatagatct ggtaatcacc ggtgacggtt
    22681 gggtgcagcc aggtgcccgt agataccagg aaagaagcta ccatctcatc accatcaaag
    22741 gcgtaaagca tttgactgct caaattgatg tcgatccagt gcccttcatt gtccgtgcgg
    22801 atggcaaccg gttcttgcgc agccggcggg taggttggcc agggggtatc tgtgggcaag
    22861 ggagtgtttg tggcggtggg tgtgaaggta ctggtaggcg taaaggttgg cgtggcggtt
    22921 ggtgtggcgg ttggggtggc tgttgctgtg ggtgtaaggc tgggctttac cagcgcgtcc
    22981 gcggggatgg gggcagcgga actgcgcgcg agcgcaatcc aattgggtac aatggcccag
    23041 atcacgaaag ctgccagcgc gatcagcacg gcgaccagca agaaaatcag ccacttgcgc
    23101 tgggtttttc ttctcagcac gggttttctt gtacctggcg taacagcctg tttcgctggc
    23161 tgcggttgac cgggtagctc gcccttttca cggggagcag ccgctatggg acgagggctt
    23221 gcaggctgag cgccatcagg tttcgcggcg cgaacgcgga tgggctgggt ctcatctgcc
    23281 gccggacttt ggggctgagg tttcgcggcg ggagtttcct caagagaagt tcgagcgaga
    23341 cggttgagcg cccagcgcat gcctttggtg gcccactcgc tggtggggtt gatcgcgagc
    23401 gccttttgca ggtattggag gctgaccgct ggtggtgatg ccgcagccag cagcaaccag
    23461 ggctcttcgc gctcaggcgc gatctgggca gcctggtgcg cccactgcct gacctccgcg
    23521 aagtcgcggt ttttcaaagc cgcctgggcg ttgagaatgg cctgcctgta agcgggaaaa
    23581 tcctgtttat tcatggcacg ggagtgggct ggacgagggt gacgttttca ttgcgaatga
    23641 agcaaagcag cccatttaca attccctggg cgaccaggtc tgcgttttca gttaagattt
    23701 gccggtcgag gttcaggaag ccggtctcga tgataaccgc cgtggtcgtg gagtggatct
    23761 catcaaaggc gtgataatag gtcatatctt ctgaggccgc gttgggatgg taggtgagcc
    23821 cagttgtctg cgcataccgg tcagccatgc acagggtaag gcgggtggct ttatcaggat
    23881 acacgctatg gatggcggcg gctactttga agcctgtggc ttcattgctg atgaaatcac
    23941 aggtatctgc gtggatcgaa accagcgcca gcgccttgta ctgcgaaagt cggtcgtcga
    24001 attcctccag caggtcgact gtgtaaccct cggccatgag tttctgttgc accaacgagg
    24061 cgatcttcag gttgacctgc tcttcggtga gcccatccgg gcataaggca cctgaatcgt
    24121 ttttatagtg accagagacg ataccaattg gcagcgcagg cgttgccggc gtgttcacct
    24181 gaacaggagc agaaatgcgg gggtttgcct gccacgcctg gaagatgcgg tcaaacatct
    24241 cgccagaaaa aagattgttg ggtgtgaaca gggtaaagag cgaggctaat agaaaagcgg
    24301 tgacgacgat cgactggatg ctgctccata tacggaaggg ttgatggcta tcctgttctt
    24361 cctcttccga ctctggcaat agggattcct cctcttccgg cagaggttca aagggcggct
    24421 caacatggtc aaacaatgaa tcgtcgctca tccaatcacc aggataaatt atagcaaggt
    24481 ttgtgccaga tgaaggtttt tccgcgccgc gacgcttgac gtaggcccgc ggcatgagta
    24541 taaaagggag aggagaatta gattatgttc actcgaaatg atcttcggca ggtaatgaga
    24601 cgctggccca gcggagtggc catccttaca acttcagatg ggcttacgac gcatggattg
    24661 agcgttaatt ctttcacttc tgtctctgtt gaccccgaga tggtaacagt caccctggcg
    24721 aataccacgc gcgctaaacg cattgtggat aggaccggcc tgtttgccat caacttgctt
    24781 cctgagacac aggcagaact ctctgatgtt ttcgccggca gaataccaga acatgctgat
    24841 cgcttcgcgg gtgttgagac cacgcctggg ctcacccaga tacccctatt aaaagccgct
    24901 tctgcgcatg tggagtgccg ggtcgtccac acttacgaaa tgccgcattc taccctgtat
    24961 gtagcggaag tgctgcgcgc ggagaaaaac gcagacgcgc cgccgttggt gtactttaac
    25021 cgggagtacc ataggataca gtaatgacca atccactaac agaagaagaa aaacactacc
    25081 tgctccagct ggcccggcga accattgaac aggcgttaaa acaggcgcca gaacctgaaa
    25141 tcgatttagg gatgctctct gcagcgctca aggaacctgg ggcgtccttt gtaacgctta
    25201 ccatccggcg taacctgcgc ggatgtatcg gcagcctgga agcccgccag gcgctggctt
    25261 ttgacgtgcg cgaacacgct cgccaggcag cctttgaaga ttatcgtttt cctcctctct
    25321 cgaaggctga attacccgcg accagcatcg aaatttcaag gctgactgca ccgcttgacc
    25381 tggaatacca acaaccatcc gacttaccgc gaattctgcg gccaggcatt gacggggtga
    25441 tccttcaaga tgggtacagg cgggcgactt ttttgccgca ggtgtgggag cagctcccga
    25501 atgtggatga ttttctttca cacctctgcg taaaaatggg ttccccggct gacctgtgga
    25561 aaagaaaaac gctgcaggta aaaacctaca gcgttgaaga atttcatgaa taaaggaagt
    25621 tctttttctc tcacttcgag agtgatctag aagtggtgtg tttcgacaac attcaatacg
    25681 aaaatcctgc aacgtttttg tgatggatca gtggtttcag gaaagcaggc tcttatcaaa
    25741 gccagtgcgt tttcaagctt ctcgtcttcg gcaacgcaaa gcagggtcga aagtcctctt
    25801 ctgagaaaac cgcccgtcga ggcgatggtg gtgacgcggt agtctccgtg ggtcagggcg
    25861 gacgtgacca tttctgaatc atcatcattt acgatagcaa tgatcatttt catgactata
    25921 actcctcgta atgttcgatc tcaagggcga aaacggtggc gccgcccaca gtcacagggg
    25981 taggcgcggg caggggcagg ggggcgcttt ccagcggcac agcgatgtat tcgatacgtt
    26041 ggcggcagtt atcatgcagt gcgtttagta tctgcggctg ctgttcttgt ggaatgccaa
    26101 ttatcagcgt ggcgttgcgc cgacctaaaa aagcgccggt gctcggcaga cggatgaggg
    26161 atatattcat atcgttgagg atattttcta ccaggtcagc gtcctgcgcc tggactaccg
    26221 ccatcagcat ggtgttgggg gtggatgcgg gttgttcact catcgcccat ctccttcttc
    26281 ctcgctcatt ccagggttat cctgctggta cctggctgat tcaggtataa aacttctcca
    26341 gattgcaggt gaatttcagt caggtgatgc tttaacagca gttccaactg attatattgt
    26401 accgtaactg gaggttgttt tccatcataa tgcctgatga ccaactcgcg gttggataat
    26461 ttcatcagac cgtgcagtct aagaaagagc gcagtaggcg caagctcggt tattcgtgag
    26521 gttgaacagc gagaacggct gccctggtca gatggccctt ttcccaggtc tttgagaaaa
    26581 tgattttgaa acaactggtc agcctggtcg aatttgccgt atctcaccag accatcgatt
    26641 accattgcag ccaggtagga tgggagcaac aaaccaggcg ctggctctga taccgggaag
    26701 acagccaaac catggtctgt tagataagat ttgactttgg cgcgcgaggt tattctgcgg
    26761 gagtcttttt cactgagaaa accagcataa caggccataa gctcaaaaac gcttggggaa
    26821 ttttggtcca ccaggctgat ctctccgcgc gccccctctg gcagcctctt gacgcttatc
    26881 gattcaatga attcaaattc ttcgcgcgga acgtaacaaa acgatgaacc gactggctga
    26941 aaatcagcgg cagtgattct cttctctaag taggtgcctg ctgcaaaacc ggttagacaa
    27001 ccgttcaact cggcaggaag ccttccctgg taatcgagcc gcaggcaaat cttcccgggg
    27061 tgaggtagat tttgtttgaa ccgggggaag ccatcattgt ggaaactggc aagaaccagc
    27121 ctggtggttc ccggggcaga cgggtggttc attctgtccg tgggtttcaa caaatctgtt
    27181 cctgcttctg ccaaccgggc gcggatttgt tccagccaat caagcgcatc attcatttct
    27241 atcacgcggc caatgcgcag tagctcagat acctcacggt aaagcagcga caccagaaaa
    27301 aggttgcgag gatgcgcaag cctgaaccac agttcaacca ggtctgcctg agtatcgggt
    27361 gaatgatcat taaatccaag ttgcagcgga tgggtcaagg cgctgagaac caggctcccc
    27421 gagtcatcta ataccaccca ggtttttaaa aaagcgagca gctgggggta gatacccctc
    27481 agccaggtga aatcatccag gtagggctgc agctcagata cgagcgaggc cagcatgggt
    27541 aatgatgaat gaccagtaag cgtatggttc acgctggcgc ggagatcaat ccgaccgttg
    27601 ggttgctgaa ggttgaggaa attttgcaac agccctttga gcgcttcagg gcggcctggc
    27661 aggagattgt gagcgagcag gtagatttcg ggcagggtct gaccgttcca ctctggctgt
    27721 gcgtcaagga tactttcaga aacgtggtag ccgagatctg gtgagcggtt ggccacgtag
    27781 gtgatctggg tgtgcttttg cagcgggggc atgagcaact ggtgagttcg atcctgcgca
    27841 tgctgcagtt tatcatcaag cgcagcttgg gcagaggtgc aatgaacttt cgaaccttta
    27901 tcggccattt ctattttgat ttgttcaagc tcaagcgcac tggaagagaa ttgacgcgcc
    27961 tgttgtgtgg aggattcaac agaggtgagt gaagccaacg cccacgtgac ctggcggctg
    28021 gtgcctggca tcaggagcat ttgattgccc aaccctggaa aatcggtcag agaggccatt
    28081 gctccaccgg taaggtagaa aaccgggcga aggttggcgc attctccctg catgatggta
    28141 ttcaagcctg cctgcgcgtg tttcatgggg ctgccgcctt gcaatggctg cagatgaacg
    28201 cgccaatcaa ccactacttt gagcaaagct tgccccgtgt ttaggcagtt catttgacca
    28261 accaccacct gcgggtgagg aacccagacg cggtattcaa cgtctatgga tggaaaagga
    28321 gagaaggtaa atttaaccaa acaagtggat gaaaaggaaa ccaccgggta tgcgctgaac
    28381 ctggctgggt cttgaatcac caggttttcc atggagaatt gtggaaaaac gtgcatgcgc
    28441 gaggcgcgta acccgtaagt tgtgagcagt gataacgcct ttggttctgc cagctctgtt
    28501 ccaatcgccc agcattgatc atccagaata ccgggttttc caaagcgcat atccacgcag
    28561 agaaagcggc aaatcgcttg tggtatgact gagggtagca ttaaatctta ttgtatgagt
    28621 acacgcccac gaggtcaatg gaattatagt agaatcaagc catggctgta caggataaag
    28681 gtgtaaatga cagatgatgt gaatacgccg gcggatgaat ccgcggtacc ccaaaaaccc
    28741 atcgaccctt ccttgccgaa cattggtcgt ttccagaacc tgccgaatcc tgaagaaccg
    28801 ccgggctaca cctggcaatt catacaagat cgcagccatt ctcaggcgac cgtgccggta
    28861 acgccttcca acgaggaaca agtggctgat agcaagacgg agccatcttc acctcaccct
    28921 caggatgaac agaactctca ggggccaagc ggtgatacag aaccccatcg cctgagactg
    28981 agaacttccg atacaggcgg ggaaaaggct gaaagtgaac attttgaaga cctgctcaca
    29041 gtacctagcg tttcgttacc ctcaataacg agaaaagaag aaggttatcc tccccgcaag
    29101 gtcattccgc cagcaggggg gcgcgatgga ttgccgcgca aggtggatga agtggattta
    29161 tccgcgacta ttgccacccc tgccgcgctc caggtgaata gaactggaga gaaagcggcg
    29221 cgaaaactgc ctgccgcgcc gcctgcccgg gcgaagatcc ccgcaatgaa caggagaaaa
    29281 cccacccgtc caaggcagca aaaaaaaccg ggctttgatt atggcggctg cctggtcaag
    29341 gtggttgtta ttctgttgtt tatgcttgtg gtgggtgtta tctttgctgg tattgtcttt
    29401 gtatatcaat actacaccat cgcttccacc ctgcccagtg tggaggatat tcgcgggcac
    29461 gcgtctcaat ttgaaactac gcgctttttt gatcgcaacg ggcaggttct ctatgaaatg
    29521 atcgatccga acgcagggcg tagaacctac accccgcttg aagatatttc gccgtatgtg
    29581 ttggcggcaa ccatcgctac ggaagataag gaattctata atcaccctgg ttttgacctg
    29641 ttggcgctgg tcagggcgat ggttaccaat tacaccagcg gagaggttgt ttctggcgct
    29701 tccaccatca cccaacaact ggcgagaacc ttattcttta cccccgaaga acgggttgaa
    29761 atctcctacc ggcgcaaggc gaaagaaatc attctgtctt cagaaatcac caggcgctac
    29821 acaaaggatg agatccttga actttacctg aacgagatta attacggcaa cctggcgtac
    29881 ggtattgagg cggccgcaga gacgtatttc aacaccacag cgaaagaact taccctggct
    29941 caagcagcct ttttggccgg gttgccgcag gcgccaagtg tgtatgatat tttctcgaat
    30001 cgcgacatga ccttgaaccg caataagcag gttcttaccg ccatgtacaa cgtcagtaaa
    30061 gagaaagact gtatctatgt gagcaacagc ccgcagcgta tctgtataga ggcgcaacag
    30121 gcagctgacg cctatatctg gatcgacaat tatgagttta agttgaaggc aaacccaatg
    30181 gtatacccgc attgggtcac ctacatacgc tatctgcttg aaaccatgta tgacccgcaa
    30241 atgatctacc gttcagggtt cagggtattc accacgctcg acccgcactt acagcttgag
    30301 gcagatcgta ttgtcagaga acaggtggaa gcgctggcag accgccatgt gacagatggc
    30361 gcgttggtgg cgatcgaccc ggaaagcggg gagatactcg ccatgaccgg ttcagcggat
    30421 ttttacaacg aagacattgc cgggcagatc aatatggcgc tgcaaccacg ccaaccgggt
    30481 tcatccatca aacctctcac ttatgccgcc gcttttgaaa aagggtggac gccggctact
    30541 gttttatggg acgtgccgag tgaatttcca ccttctggcg acccgaacga cacacgtgac
    30601 ccttatatac cggtcaatta cgatggcaaa ttccatggac ctgtttcagt aaggtcagct
    30661 ctggcgaatt cttataacgt cccggcggtc aaggcactgg aatttgtggg aatttatgac
    30721 aatcctgcaa cagttgaagc ggatggattg attgcttttg cccgccgact gggcatcagc
    30781 acactggtac gcaatgatta tgggctatca ctgacgctgg gtggcggtga agtaaccctg
    30841 ttcgatatga ccagcgcttt cagcgtgttc gcgaacaacg gcagaagagt agtgccggtt
    30901 gccatcaccc gtattgagga tcataaggga aagatcattt atgaatcgaa cccgcgagaa
    30961 cctgaacagg tgatgcgcgc agaacacgcg tatttgatca cttccatcct ttcagatgcc
    31021 atggcgcgcg cgccgatgtt tggctcggat tcgatcttaa acctgccgtt cccggcggcg
    31081 gttaaaacgg gcaccaccaa tgaataccgc gataactgga ccattggcta cacccctgac
    31141 ctggtgactg gagtatgggt gggcaacgcg gataattctc ctatggtggg cacttctggt
    31201 gtatctggcg cggcgcccat ttggaatgcc tttatgaatt cggcaatacc ttacctcact
    31261 ggcaacgcgc ctgccaattt cacccggcca gaaggcatcg tggaaaaaga gatttgcgtc
    31321 tattcaggcg cagaaccatc ggaaaattgc actgagcgca ggagagagct gtttgctttt
    31381 gatcaacctc cgccttcgag cgaagatgac ctgtggaagc tgttgaaggt ggatacctgg
    31441 acaaacctga aagcctcaag cgcctgcgaa ggcttcgctg aaaccaaact ggtgttgaat
    31501 gtaagcgaca agtgggcgaa aaaatggatc gacgacacag accagggaag agcgtggctt
    31561 gagaaaatca acttctctcc gccggttatc tacgtgcctt caagggaatg ccgagcggat
    31621 gacccccgcc ccactttatt gttcgtggga ttgaatgacg ggcaaaccat caccacggat
    31681 aaattagaca tttacgcagt tgtggacgcc accaagaact ttaaagaata ttacctggag
    31741 tacggggagg ggaaggatcc gcacaagtgg acaacgctgg tggaacccgg cggagcaact
    31801 tctgccgacc cgcagaagtt gctcacctgg gacctgctca aggtcaataa tggggtggtg
    31861 acgctgcgtc tgttcatgct ctccaccaaa aacggacacg ccgaaaaact gcttcggctc
    31921 aacctccagg tgcccactcc cacacctacc aatacgccca cgccgaccga gacgcctacc
    31981 attacaccga cccctacgag tacggcaact cccacgccta cgcttacgtt aactcccatg
    32041 ccattgttcc caactcttga tgcattcctg cagacgctgt taccgcccgg gaattgatga
    32101 gatacggtgt cgcttatctt cgccgcgggc agatgtgaat gggatcttgg gatgtaaaaa
    32161 aacggtgcag tggttgccct tccaacgaga gcactctgct gttggcaaaa aaggggaagt
    32221 gaaaatcagt gaaatccccg gccgattttc cggggatggg catgagcctg gcggtgagcg
    32281 gtttgtcaag ccggatggat atagcagcga tgtccatcaa tactgaccgc aactgctcaa
    32341 tggaagtatc gcccggcagg gggatggtat ccaggccggt accgcagagg gttgaataaa
    32401 gcagcaggtc tttgacgttt agttgtccct cagccgccct ttttgccagg atggagtctt
    32461 ccagcaacgg cagcatcatg ccattaaaac ctgccttacg ccagttacct ctatctaatg
    32521 tatctgcgat gagggcaatc gcaggcagcg tgcccaaccc gccaatatgc gccagaccca
    32581 ggcgttccgc cgcgcctgcc agtgaacacc agtcttctgg gaaaggcgct ggagagaaat
    32641 cgaacccttt gaatcggatt ccgccgctgt ggatgatggg tagaatgacc tcctccaact
    32701 ggctggaagc gttttgtaac gagtcaagaa gagactgcct gcattcctcc agggtgaggt
    32761 cttgcgaaaa agcgtcgttt accgcgtccg cgcattccac agcgattgag atggctgggg
    32821 ctgaaccagc ctggtggtat gcagcaggta gaaaaggcgt ccagggttcc acgctggcga
    32881 gggcagcgaa gcgaaggttg gcgaagccat cggtggaatt ttgcgcggtt tggtgaatga
    32941 tctgcgcccc cgctaaaact gcgccgggat aaagcgtgtg gttcgcggta atgaccgcgc
    33001 taaaaaaaac gtttttcgtt gagcggatca attgagggat gagcttgtat gcccagggat
    33061 gttctggcag tgccggaccc attgtaagat attgccaacc cagtgattgc gccttttgtt
    33121 ccaattgtgt agcgcaggag ataacctccg cgggcggacg gtcattgagg tagcaggaaa
    33181 atggcgaggt gcccaggcgg gtggactgca cttccagctt ttgctggata gcctccctga
    33241 gcgcttcgct aacctctgcc aggcgcttta gctgtcgttc tgcctggggg caagcggggt
    33301 cgtagaaact ggtgatagaa cggaccttca tgcgcttctc tggcgtaaaa cttcgtatag
    33361 caatagagag gcagccacac cagcgttaag cgattcaaag ttgccgggca tggggatgtg
    33421 aatggcggca tctgaaagcg cacgcgcttc ctttccagcg ccttcagcct cacccccaat
    33481 gaccaacgcc agcggtcggg tgaggtcagc ctgccatatg ggcaaaccgc tgccagcgtc
    33541 tgccagcagc acctgcaacg gcggctgcgc ctgccgcttg cagtagtctt cgattccatc
    33601 ccaggaggtc tggcagatgg gcagatgaaa atgcgctccc atgcctgcgc gcaccacctt
    33661 tggcataaag gggtccacgc taccgggggc caacaacacc gctcccgccg cggctgcggc
    33721 ggcgctgcgt agaatggttc ccaggttgcc tggatcgcgc acctggtcaa tgatgagcac
    33781 aggttctgcc acgcctgtga gggatatcaa ctcttgcggg agaacagcga ggatcccctg
    33841 ggaggtttcg gtgtcagaaa gccggttgag gatatcaccg ctcacttcaa aggcttccac
    33901 accagtctgc cgtaaccgat caaccagttg tatcccccgc gccgagagat tcacactgaa
    33961 gtaaactgac tggggcagga aatgacgcct gaaggcttct tcgaccaatc gcacgccttc
    34021 aatgacgaat tgaccggttt catcacgata agaacgctgc gagatcaagc tgcgcaggcg
    34081 ctggactttt gggtttttga acgaggtgat cacttccaaa gcaagtctca ttcaataaat
    34141 tctactcgaa tgcaggttaa aaacaaaacg ggatggggtc agccatcccg cagttgaata
    34201 tggaaggttt acgccgcctt ggctttttcc accacagcgc tgaaagcctg ggggtcccgc
    34261 gcagcgaggt cagcgagcat cttgcggttg agcaggatgt tggccttgcg cagaccatgg
    34321 atcaactggc tgtaagtgat gccgttttgc cgggaacctg cgttaatgcg ggtaatccat
    34381 aaacggcgca gatcgcgttt gcgcatgcgg cggtcacgcg aggcgtacca catgcttttc
    34441 agacgggctt cgttggcgcg acgaaacagg cgatgccggc tgccgaactg cccctgggtg
    34501 aatttcagtg ttcttttatg acgttggtga ctggtaaagc cacttcttac acgagccatt
    34561 ctttactcct ttgcgagccc ctgggcgccg gtcttgtgac ccgttgtgaa cacccaacag
    34621 ccgcactgat gcggatacga caggtcagcg cctctcccta cttcatgttg gggttgagac
    34681 gtttgacacg ctggatgatc ttatcacctt gaacggggac catctcagtg aacaatgcct
    34741 tggtgcggtc agaagtgcgg cgcctgaggt ggctttttcc gcctttggtg cgcaacaaga
    34801 cgcctgaacc cgtcaggcga aatcgcttgg atgtcgcttt atgggtcttc agcttgaact
    34861 taccggtttt catcttgtgt ggcacagctt actcctctca aacaaattac gcgtgctttg
    34921 agcgcgaacg cgaattatac cagaaatcaa ttattccggg gaattttcac cgtcgtgata
    34981 gcggtcggtg cgaaaaactc aggctttttc agcggtcttc tgaggttctc ctggcggaac
    35041 ctcattcgcc ttatcctcgg cggcgtcttt cttttccgga gcaggagaag gttttttctt
    35101 gccgggtttg gatggcgaca gcaccatacc catagttcgt ccttccatat taggcgcctg
    35161 ttcaatggtg gcgatgtcgg ccagttcttc cgcgatttct ttcaggtctt ccagcgcaat
    35221 ttccgggtag gtgatctcgc gacctctgaa acggatggtc acttttactt tgttgccttc
    35281 caacagccag cgcctggcat cgcgggtttt aaatccgcgg tgatgttcat tggtttttgg
    35341 gcgcaaacga atttctttga tctcgatctt ggtttgggct ttacgggctt ccttctcctt
    35401 cttggttcgt tcgtaaagga atttcccaaa atccatcaca cggcagaccg gtggctcaga
    35461 gagcggtgct acctccacca ggtcgagctc agcttcacgc gcgatttgta acgctttctg
    35521 gataggcacg accccgatat tttcattttg gggtccaatc aaacgaactt cagcgacctt
    35581 gatcaactca tttacacgga aattttgggt actgatggtc ttctcctgtt agatagattg
    35641 atgacgtgcc cgttccgtta acgggcaaaa aatgattttg ataataccat aattttgaat
    35701 gactagtcaa ccgggttggc aggacaattt tcacgtttac aggtcgataa attccaggtg
    35761 ccggcgataa ccagggtcaa tttccgcgta ttttccgcga tactcttccg gaagcaagcg
    35821 ggcgatttcg tacatgatgg cgtcggtcaa ttcccggcgc ataagcttat cagggttgct
    35881 gctctgcgcc tttaaccgga atggtgcacc ggccttaagc accatcggcg tacgccgata
    35941 cttgcgcagg ttaagttgga tattttcgtt gccgtagaaa acaaaaggca tgatcggcgc
    36001 gttggtgcgc agcgctaaaa atacaattcc tgggtaaccg gtattgagtt tcccagtctt
    36061 tgagcgggtg ccttctgggg caatggcgat gattttatgc gcagccaacg cttcttcagc
    36121 cttgcgaaac gcgttaaaat caacctcgcc acggctgatt gggatggctt cccaggcgtt
    36181 aaagagcagc cgcagccaat aattgtccca ggtttcgatt ttggcgaaag cggttatctt
    36241 acgcgggtgc agatggctga accctaccgg cgcgtcaagc cagttgatgt gattggtggc
    36301 catgatcagt ggcccgctca tgggcacgga gtggatcccg ctgtcatcaa ttcgcatgaa
    36361 ggcgcgcagg atggttttaa tggggggatt gatgtgcgca gcggtgagca ttaattctca
    36421 agccgagtga tgatttctag tgaatggggc aagagttcca ccgttatttc atggcatgct
    36481 tcgccgactg tgttgccatc tgcatggacg ggcagggcac ctttttttgc gattatggtg
    36541 attctatctg ccctggcgac gctgacggct gggtcagact catgtgtgcc tttgatgaac
    36601 ttggccatga gtccgaacat ggagaactga ggtacttcgc gcaccatgca gatatttaat
    36661 tgaccatctg tgatggaaga ggagggcgcc atcataaaac cgccgcccat acgcctgccg
    36721 ttcatgatgg aaaccattaa agccggaagc gtcaaggtct tatctgcgaa cttaatctcg
    36781 acgttgggtg gtttgaaata aataaaaatc gtctttatgg ctgcaaccag gtaagaaagc
    36841 atgcctgtta acttcatttt ggcggccatg aagcccacca tcgcgtcaaa acctacgccg
    36901 atgccattgc caaagtacat gccttggggg gcgtcacccc cgctgacatg gccgatatcg
    36961 atccattttc gcgggagaga tgccagcaat gtgcaggctt catctaattc agtgggaaca
    37021 ttggccccga aggcgaaatc attgccacgg ccaaccggga ggatacccat cactggcaaa
    37081 aaccctccag cggcgcggtt ttgcatcaac ccgttgagca cttcgtttgc ggtgccgtcg
    37141 ccgccagcag cgatgacgta atcccatttt tcttctgcgg ccttgcgggc aatttcagtg
    37201 ccgtgaccag gatattcagt caccttgata tcgaactcaa ggttatactg cgcaagcgac
    37261 tctttaatgt gcgtgaggga gcgtaaggct ttgccctttc cggcgattgg gttaaggatg
    37321 atcaggtaac gtgacatatg gctccggttt tctaaaaaaa caaaagtgaa tggtttctgt
    37381 caactgcgct caatgatacc gcctttgttg taccttgtta acactccaat cccacgattg
    37441 atgcgatttt tccttgcgat cgatgataga aggaagccaa tcgtgcatca attaaaaaaa
    37501 acacccccgt tttggtgttt tgtggtgggc ggtataggac tcgaacctac gacctttgcg
    37561 atgtcaacgc aacgctctaa ccaactgagc taaccgcccg gtgctgctta ttataatgga
    37621 ttttcattga ttgacaatag cgattggtga gctgatggcg cgaccgatta gtttaaagga
    37681 tgttcaccac cccggcccat aaaacgatga acctgggttg cggtagatca tgacctggcg
    37741 gagacagatg accaatggcg atgatcaact tctgtccttt ctgaaaaccg gtgaagcgcc
    37801 ttgagccata cataggaacc aggttggcgg ggtcaatggt gtacaaggtg caatccggct
    37861 cgtaggctgt tgcgtatagc tggtctttgc cgatcaccaa ttggatgatg ttgggagcaa
    37921 tgaagtcaac cccagacgga atggtgaact catacccctt ggttgaaagc cggaatccat
    37981 cacggccgaa gatggagtac tcttcgcctt cttccccggt tgaagagact ttctgccagt
    38041 gggcgtcatc cacaaccagt atgggtaatt ccaggtcggg tacgatggaa gacatttacc
    38101 gcaacaagct ccttattgaa agaggatgga tggtgacagc ctgcctgtct tttcttgttt
    38161 aattatagag gacattttaa aaaggaacct caaagaggag cacgaaaaat gaaatcctgg
    38221 cacttctcat ttcaaatggt ataatattgg cgcttaaggc ttcatgcacg gagaggtagc
    38281 gcagtctggc ctaacgcgcc cgcttggaaa gcgggtatac cgcaaggtat cgtgggttcg
    38341 aatcccaccc tctccgcctc actaaacccg ttcggcttcc agggcgcgaa aaccgcagcc
    38401 ctctccgcct cactaaaccc gttcggcttc cagggcgcat agactgaagt gaataataga
    38461 agggccagtg acgcctatca actcccgcgc aggcagaccc ggcgggtagt tcgttttaat
    38521 catatgggct atatgaatcc agtgaacctc tgcttaaagc gataggcagg catggggggg
    38581 acatgtctgc ctatctatag ggagggaacc ccacctttgg tctttttgac ctttggtgga
    38641 acacactggt cttgctacca ataaaagtgt gttataaaga cattgtatac ctaaattgtc
    38701 ttaaaatcaa gtggttgttg aattttcttc gcggttttct gtcatcaatt tgagcatctc
    38761 ttcaacttct tcttcagatg gaggtgtctc tttgatattc atgctcgcct gtgggctgga
    38821 aaaaaccgcg cgcatttgcc tggcaaattc gtcagagctc atcacttctg cgtgcagcat
    38881 acgggcttta ttctgcactt cgtggtccga agagaccacc agcaggttac gggcattgta
    38941 gccttctctg tggaggtatt gaatgatggc tgcgtcggcg gtggttccct gccgcacgtg
    39001 gtgaacattg atcaacccca aacgtgaagt agcgtttttc ccgggtggag cgccatcaaa
    39061 gaacagctcg gcttttacct tcgccaggcg gcaatattcg ctgaccctgg cggtgagttt
    39121 ggcttcgtct tcagggtccg aaaggctgat gccgccgact tttggaatga ggttgtgccc
    39181 gtcaattaaa atcttcttca tttcactctt gtaccagaca ttttactttg aaattatgct
    39241 atgattcttt cagaaaaacg attggattat gcggaaaatt ctcccttacc tgttacttaa
    39301 ttttgtcgtc tctgccctgg cggtgtggat cgtgctgatg gtgtgggaag cgaatcatca
    39361 aattccgaat atccccgcgg ctgaacagcc agttacccgt gaaacaaaat cacccacaat
    39421 cacccaacct ccattggatg aaaagaccat cacaatccaa ctggtgatgg gcagcggaga
    39481 tatccgcagc gaaagggtgc agctggtcag cgcttcggca tctccagtga acctgcaggg
    39541 ttgggaattg gtggatgaag acaaaaatcg ctaccagttc ccttccgtga cgctttttcc
    39601 cggcgccgcg atagagcttt acaccaaagg aggagtgaat accgctggcg aattattctg
    39661 gaatcgcgaa gaaccggttt ataccagcgg ggagagaatc cttctcaaag acaccagcgg
    39721 gaatatccgc tccgagtacc aggtacctta aaagaccttc agtaaagaag gaagagacag
    39781 ggtttcgggt aaaatcggaa tatctgtaaa ataatggaaa gacagcaaag atcggataaa
    39841 tacatatgac ccaggctttt taccgtaaat ggcgtccacg caggtgggat caggtagtgg
    39901 cgcaggacca tgtggtgcag actctcaaga atgccatcaa agcggacaga gtcgggcatg
    39961 cgtacctttt ttctggcccg cgtggcaccg gaaaaaccac aactgcgcgg ctgctggcta
    40021 aggcagtcaa ctgcctcaat gaaaacccgg ctgaacgccc ctgcgatagc tgcagccact
    40081 gcctggcggt aaacgaaaac cgttttctgg atttgattga aatcgatgcc gcttcgaata
    40141 ccagcgttga agacgtccgt gacttacgtg acaggatcaa ttactcgccc acagacggta
    40201 aatacaaggt ctacattatt gatgaagtcc atatgctctc caccgcggct ttcaacgcgt
    40261 tattgaaaac gcttgaagag cctcctccac acgccatctt cattttggcc accacagaag
    40321 tgcataaaat accggccaca gtgctttcac gttgtcagcg ccacgaattc aggcgcatcc
    40381 cggtggcaga aacggtgaag gcgctgcggg agatttgcaa agaagaacag atcgaaactg
    40441 atgaagaagc gctgctgttg atcgcgcgcc agtccaccgg cgccttacgg gatgccatct
    40501 cgctgcttga tcagcttgcc agcaccggca agcatattga cctggctttg gcgcagacgg
    40561 ttctgggcac cgctaccaac caattggtct ttgacctgat cgaggggttg cttaagaacg
    40621 atcccgcgtt gggcatgcag accattcaca aggcgctgga tggtggcagc gacccacgtc
    40681 agtatgcgcg ccaggtggtg gaatacctgc gcaacctgtt actgatgaaa atgggcaacg
    40741 agaaagaaat cgaagccacc cgggaggtga aaaaccggat acgcgaacac agtgaccggg
    40801 ttgagataga cgcgttgatg gagtggattg gcctgtttaa cgcggctgtc agcgacttgc
    40861 gcaccacctg gcagcccagc ctggcgctgg aggtagcctt cgtgcgcgcg ctgaaccccg
    40921 ctggcgctga gcgggtgccg gtgttggaga cgggcacaga aaaacagcaa tcccggtcaa
    40981 cgccagcagc agaaaaaatt gcaacccgcg aaatggccgc tgcggagaag aaggctgaga
    41041 aaaaaaatga accccctgcg gctgaggagg ttgcagacac gccagcccct ttgcctgaag
    41101 agcccgcagc gcgagtgcaa attgaacaac cagggaaagt ccatggaaag gaaatggcag
    41161 gcgcgggaag cgcagagctg accacccagg acttactggc ttcctggagt gccatccgcg
    41221 ccgaggtcaa gttgcgccgt attcaggctg aagcgcttct caactcgcag aaattgctgc
    41281 aggtaaaaaa cgggatcttg gtattgggtt ttcccagcga ggtgctccgt tccaaaatgg
    41341 aaatccctga aaatatcgag gtcacacgaa gggtcattca gaacctgctg caggtggatt
    41401 tgcccattca atgcgtgctg gtaactgaaa aaggggctat ttcatccagt aacgactcag
    41461 aaccaaatgg gctggtggat gcagccatca acctgggcgg aaagctgata cacaaagact
    41521 gaaagtagag ggagtcatta tggcaaaagg attcaatcga ccggcaggcg ggcaaggcag
    41581 tggtgggatg atggcacaga tcaagcgctt gcaagaacaa atggaaacgg cacaacagca
    41641 actggcgttg gaaaccgtta cctcttccgc agggggcggc gcggtgaagg tgaccatgac
    41701 tggcgaccaa cgctgcaccg aggtgaccat tgaccctgac ctgttaaaag acgccgacgc
    41761 agaaatgctg caagacctgg tgctttcagc agtgaacctg gcgttggatg attcgcgtaa
    41821 gctggcagcg gaaaaactcg gcccgctggc tggcggcatg ccaggcctgt tctaaaccag
    41881 gcagcccatg cccattttac ctgtccccat ccaggaaatg atcggcgcgt tggaaaggct
    41941 gcccggtatt ggccccaaat cagcctcgcg cctggcgttc tttttgctgc gagccccgca
    42001 ggacctggtg cagcaattga ccagcgcgct caccaatctt aaatcggcta ctggtacttg
    42061 ccagctgtgt gcgaatatca ccctggccga ccaggaactg tgcgaagtgt gccgcgaccc
    42121 gcagcgcgct gacggcacca tttgcgtagt agaagagccg ttggatgtgc tggcgttgga
    42181 acgcacgggg ggattccacg ggcgttatca cgtgctgcag ggcgtgctgt cacctatcga
    42241 gggagtgggc cctgatgacc ttaccattcg cggactcatg gaacgtgtac gcgccggaga
    42301 agcgaaagag gtgatcctgg cgaccaaccc cagcatggaa ggcgacgcca ccgcgctcta
    42361 tttgagacaa caactgctgg cgctgggggt gcgcgttacc cgcctggctc gcggtctgcc
    42421 agttggtgga gacctggaat acgccgacca gcacacgctg ctgcgcgcgc tggctggccg
    42481 gcaggagatg acgtaaaagg acctgggctc agctcaggtc cttttggttg aaaaagggat
    42541 acttatctct taaacccggc tagctcgatt ttctttcctt cttgttctac gatgtaataa
    42601 ggaagccaaa caaccgcgaa aatgtcgggg aagatatcgc tcttggttgg ttttatggtg
    42661 atttcactga tttcatctgc gatcccatcc catttttctt tcaccttggc gaccagctct
    42721 tctttggctt ttttcaattc tgccaattgt tccgtgtatt gtgccagtgt ggtctttttt
    42781 gcttccagtt tgtctttggc tgcagaagcc attcgatttt ttgaaagcgt gctgctgatg
    42841 ctgcgttttc gcccagtgag catgccaatc accaccgcgc caccggtggc catcgtttca
    42901 aggcggcgtt gattcacagt ggattcagct gcctttacct ccagctcttg cgattgtatc
    42961 ttgcgctcca tggcggcaat cttgctgtca gccgccttgc tggctttttc aatttcttcc
    43021 tcaaccgttt tgtccaccgc gtcttgaaca ctgcggcgga actcggcttc aggtataccg
    43081 ggtgtaccga agagttgaag cttattattg gccaatatct ttaaaccgcc gctgcggtaa
    43141 atccaatcca gaaagtcctt tttaagttca tcccttttac gcgcatcaga cagccaggat
    43201 ggaacaggat gaaacttagc cttgggcagc gggtgcattt ccagcctgtc tttgtcgacc
    43261 tcactggaaa caagctcgtc ccagcggacg agcccgctgc ctgggtcttc gagaatcgcg
    43321 caagttttgt tgatctgttc aaggttgtac tgcctggaaa caaaccgcac ttcagcctgc
    43381 gctatgatgc ctggtcgata agccagcccc tcagagtcga gcgcgccaga gaattgggct
    43441 gttgccgcgg cttcgctgat gcccaggttg ttagggtaaa agtattccgc aatatcaccg
    43501 gggatcgccg gttggctgga ggttagacca gagccttcac ggactgttga ggtcgttttt
    43561 tgcgccggtt tgaccgtttc agttggcgcg ccagttgcgt gggcgttgac accagcaagc
    43621 gcgttggctg ctgccagctg gtcgcgcgtc atgggtccag ccaggaaatt catcgcccag
    43681 cgcgtattaa agagcactgg cttggagcgg tagacgctgt gcagcatgaa tacccgtttc
    43741 tccagcccgg agatgagatt gtcaatggta cggatgtccg tgctgccaga ggcggaagac
    43801 agcccgtcca tcaggcgttg tttgtccagg ctggtttgta accggccaat catccaggtg
    43861 ccggcgttcg aaagcgcttt gtaatccagg tcaagcggat tctgcgtggc aatcaccagt
    43921 cctaccccga aagcgcgcgc ctgtttgagc aagcgcagca acacaacctt ggagggtggg
    43981 ttggggtttg ggctagacgc tgggggcagg taacccatga tttcatcaaa gtacaggatg
    44041 gcacgcagat ttcctgtacc gcgctgtccg cgcatccagc tttcgacggc ggcgaaaagc
    44101 agcgtcacaa agaacatgcg ttcattctcg ctcaggtgcg ccaggtagaa aatggagtgg
    44161 cgtggaccgc catcagcagt gtaaagcaat gcgcctatat ccagtggttg accttgctgc
    44221 cagacctgga aggaaggcga agcgaggaag ttattcaata gaagcgccag ttcgaagcgg
    44281 tttttctcgg ggaagaaaga ttccattggg aaggcgccga gtcgatccat tggcggattt
    44341 tgcacttgca agatcaattc ggtgagcgat agggaggtgt catggatcca ggcgttttca
    44401 atcaggctcg agagcaagat gtgctcgcga gagcgcagcg ggtcaatgtt gctcagaccg
    44461 attaacccca gcaccgcagt aaccgtggtg gagatgcgct cgcgcaatac ctcggcgttt
    44521 tcattgaacg ggatctcggg cgcttcaaat gatgagagga tgttcaccgg gattccggat
    44581 gtggagccag gagaataaat ggtgtattca gcggcttcag ccaatgcctg caatttttct
    44641 ttgccgattc cccattctgc caaacctttg ctccagcggc tggcggtttc ttcagccatt
    44701 tgctcaacgc ttttaccctc acgccgggcg gcttcagggt cgatccaggg ttggaaatca
    44761 gccggctgct ggttgggaaa atgcagcacc agattggtca ggtcgccttt cgggtcgatg
    44821 ataatcgccg gggttttctt taaggcggct tcttcgagca aatcgatgca taaacctgtc
    44881 ttccctgaac cagtcatacc ggtgacaacg caatgggttg tcaggttcgc ggagtccagc
    44941 agcacgtcct cttgcgttgg tcccttggtt tttgggtcgt acactcttcc gatgaagtaa
    45001 tttgaattat ctgaagacat cattcccacc acctgttttt ctttctatta taagatgaaa
    45061 aagcggggaa tcgatttatg gtaaccccgg ctcttttggc ccgtttcttg tatccgttat
    45121 tcaacaatcg atcgacaagg gcaaggaacc attttgtata acctcgaact ccattctatg
    45181 tacctgcttg gcggtaaggc gcaatcgacc ctgccgggac tcattgcgct gtctcccccg
    45241 cgcaaatgcg ctagagggcg tgaatttgac cagctggtgg cgcacgttga cttaagaggt
    45301 aaaaccagta tcacgcctga agccatgcag gaatggatgc tgaagaaagg cgccttgttt
    45361 tttaatactc caggctccac caccaacgcc atgcgcatga tggccgattc aatcaacaat
    45421 gaattgtttg acaggaatct aaaaaaagct gctgatagac cgcaggtgaa cggcacgctt
    45481 tgtatggtgg tgttgcgaag agacacgatc tattcgctgg ttatcgggca ggggcgcatt
    45541 tttatcctgg aagacgaagc cgtcatcgag gtggcagacc atgataatcc cccgggcggg
    45601 ctggggaccc accagtcatt gaattgcctg ttttcgcaac gcaatgttag cagtaacagc
    45661 aaggttctgt tgtccccgct gcccagcccc cagtggacgc ctgcgttcct ggctggagga
    45721 ggcaggctgc cggcggatgt gcttgggcgc aggctgttaa atcaatgccc gcaagaattg
    45781 cgagggatgc tgctgcttct gtcagaaggc agcggcaaga ttaccaacag tactctcatc
    45841 caggcggttc ctacggatgg cgcggtcact tcctccgcgc ctgcgatgcc tgacacgaag
    45901 ccagccatac agccggcatt gatcgacctg agggaggtag ccaagcgccc tgcgcaggca
    45961 gaaccagccg gagatcctca cccggctatg aagaatacag cggattcgcg gttacccgcc
    46021 gcgataaaga gcgggctggc gaaaggacaa aaaaccccta ttcagcgtaa gaacgtatca
    46081 gccaggcggc gcaggcagac cgggctgcag atagaccctg agaaaattaa agcccgggta
    46141 ggcagcgcgg tagatgttgt ggatgatgtg acagagaaag tgaagcgtaa aagctcttct
    46201 ttctacgaaa aattgttccc ttctgaagtt gacacgcccc gctcgctatt gttgttgctc
    46261 gctattgtca tcccattgat cgttgtctcg gcagcagggc tggtctatat tcgcaggggc
    46321 agaacccagc aattcaatta ttactaccag cagggacagc agtattttat tcagtccgaa
    46381 gcgcaaaaag aggacgagat aatgtatctc ttcaacctgc aggcggcgat gatgtatatg
    46441 gagaaggcgt ctgaatttgg ccagacggct gattcgatcg ccttgagttc caccatccag
    46501 tcccagcttg acgccatcca gggggtggca cggctggaaa ttacttctct caacatgaat
    46561 gatgtgctca gcaaggtgaa catcacgcaa atggtggcaa ccacgaacga cctgtacttg
    46621 ctggatggtc agtctggcag ggcgctgcgt tactcgcttt ccggtaatac ctatatcaaa
    46681 gacgaccttt ttgattgtgg accaaacccc gataacccgt taaatagaat tggggcgttg
    46741 gtggatattc taccgctccc cgccgggaat tcttttggcg caaccctgtt cgggattgac
    46801 gcctatggca atattgaatt ttgtatcccc ggtaaatcgg gcacgatttc agcgctcatt
    46861 gcgccagaag tgggatggca gggcatcaag gcgatagcga tgtatcagaa ttatctgtac
    46921 gttttggacc ccgggaataa cggggtgtat atgtatcctg gcgccggtat cctgtttgaa
    46981 gaaaagccca cattgttctt tgaccattcc atccctgacc tgaagaatac ggtggacatg
    47041 gaggtcaacg ccgatgagct ttaccttctg cgcgcgaatg gcgagatggt cgagtgcaca
    47101 tacagccata tgaaagacta caaactgaca gaatgcgaag acccggcacc gtacagcgat
    47161 atgcgcagcg gaaacgaacc acaggccatc acatttccag acgcgcagtt cgtgcagatg
    47221 cgcctgactg ccgcgcccga ctcatccatc tacttactgg atacacacgg gagcagtttg
    47281 ttccacttca gcctgcagcg caacctgcag aagatcatcc aacccctttt tgtggattcg
    47341 ggttaccgtc cgaaattcgc ggccagcagt atggctatct ctccgagcaa gatggtattt
    47401 ctggcgtttg gtaaccagat tttctacgga ccgttgccct gaagttgagc tgaaattacg
    47461 cgataacgct gagcggcacc ggaatgaagg tgagtaaaaa gatcaccaac gcggtgaagc
    47521 ccagcgcttt acgcagcggg tcaagctgtg tgatctggtc aagcggttcc gcgtaaaccc
    47581 tgccgaagag gaacaccagc atagcccaca gccaccagcc cgaccaaacg aatcccagac
    47641 ccaccaaccc gaccagaata aacggcagaa tcctgcgtga ccatttggcg ccaaacagca
    47701 gctggaagat gtgacctcca tcgagttgac cggcaggaat aaggttgaga gaggtgacca
    47761 gcagcccggc ccagcccgcc caggcgaccg ggtggatcat cacatccagc ccacccagcg
    47821 ggaaggggcg cccggtgaag aagtaacgca gccagtagag caacggtgcc gtgttaccat
    47881 aactggctgg ttggggcagt acctgaccga aatggatgta tttgagcaaa agataggtga
    47941 gcgaattgcc ttccatctgt acgccgtgcc cgggtggaaa atgaagcggg agcgtatcca
    48001 ggcgagagag cgatagaccg ataaggatca ccagaatgga aacgatgtag ccggcgatgg
    48061 gcccggcgac acctatgtcc attaaaacac ggcggttgcg cgggatttcc ttcatgttga
    48121 taaaagctcc catggtcccc aacggggtga acggcagggg gatgaaaaac ggcagggtta
    48181 cgtgaacgcc atgggcgcgc ccggcaaagt aatgaccaaa ttcatgggcg gcgagaatgg
    48241 ccagcaggct gacggcaaac ggccagccgg cgcggaaaag ttccagcgcg gctgtgaaag
    48301 gctgcttcga gagttcacct tcaaaactgg ttaacccgcc tgaaacgagt acgctcacca
    48361 gggtgagcag gaacatcagc aggttcacca gcgggcggcc aggtttaggt gcaggcaggg
    48421 cgctcaccag gtagatcacc tgttttttct cttcaaccct gaaaagaggg gttaactggt
    48481 agggtttgag caaagcagaa agctggtcat aagcagtcgc tgaatcgggc tgcaacaaat
    48541 gtccgcggta acgcacgcgg taggcctgtt gattggtacc ataggtgata tcttcaatct
    48601 ggaaaacccg gcgcacgatg tgctccagaa tttcgctatc gtctgatgac tccattttgc
    48661 tccattgtat ctttaaataa tcaggcggga atggatggga gtcgaaccca ccgcggcccg
    48721 cataacgacc cgccaccgat tttgaagacc ggggaaccca ccggggctca tccactcccg
    48781 ctgttaagaa taccgcagcc aatttgattt ggcaaggtga gtaccgctat cttcctggct
    48841 ggagattaaa aaatatacgg gaaggtcgcg ctcaaccagg gcgccaattg cagaaccagc
    48901 acgcctgcaa gtatgccgag cagaatgccc gcgacgatat ctgaaaaata atgcacgccc
    48961 atccaaaccc gcgccaggct caccaatggc gcccacacca gcatgagtaa accgaaccat
    49021 aagggaccga taaccaggcc gatcactgcc agcatggcgg ttctggcggc atgcccggaa
    49081 ggaaaggagt gtgggtcggt attgcggtaa atggcccccc actttccctc ggggcggcgc
    49141 ctgcgaatgg tgaacttgat ggcgagcacc accagcgcca ggcccaccgc accggcggcc
    49201 atcagcgccg tcattgagcg ccacagaccg acagacagga accaaatgat caacaatccc
    49261 aataaaatga accaggagtc gcctgagtgc gcaagaaaaa tcgcgatagg tttcagacgg
    49321 gaatcagggt tggtcaggcg caggcgttcg gttatctgga tgtcctgctg cagcagtcgt
    49381 tcaaatctca tggggcaatt ctaccgcaac aggtcacgct caacgatgtt gtgctggaaa
    49441 ggcttatcca catccatgcc gatctcaggg tagggcattt tcagtgcttt cccctgaatg
    49501 cccaaccgct gacaaacgcg ggaggctgcg ttgtccagcg tcaacatacc cgtcatcaat
    49561 agcaacaggg ttccccagcc aatcatcgac gcctgtttga tggggttctt acggcgttcg
    49621 atcagcccag accaggcact gtcggggtcg agcgcggcct ttttcctgat gcagttcacg
    49681 tctccgccgc agacctcacc gtctttaagt ttgaggtagg tgcggcgtga atctggaaaa
    49741 cgtttttcca tggcacttct ttcaataacg gagtaataaa tatcaaactc ccgcgcgtta
    49801 aaaatgcgta tgcaggcgtc gatgatttcc ggggtaaccg cgggcacgtc cgcagacact
    49861 gagaggatgt gactgtctgc cggggtaacc tgtgaaaagt gctcagcgcc gcgctggatg
    49921 ttttgcaata atgagccagt atccggcagg taggtgagcc ttttaacgca atgcaaacca
    49981 caggagtcat ccagaccaat gacggccaca gaatcaacca gttcagcgc
//
LOCUS       DAOTLW010000011        45159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_979_length_45159_cov_9.073430, whole genome shotgun sequence.
ACCESSION   DAOTLW010000011 DAOTLW010000000
VERSION     DAOTLW010000011.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 45159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 45159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45159
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_979_length_45159_cov_9.073430"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..552
                     /locus_tag="P5516_03145"
     CDS             <1..552
                     /locus_tag="P5516_03145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91465.1"
                     /translation="LLGMTITPGLYITAPTTGEVVEGIVEIRGSVPEEGFLSAEVLYA
                     YAQTGKETWFLIKRLDKVVQDDVLAAWDTTTITDGVYHIKLVVTKVGDRRNEVVVENI
                     QVSNYSRAPGQNEMGVNTQTEKRSAAVEIRAPVSTVIEKKVNPASIQRQEIQRTIIIA
                     ILAVCVALSSIAIYSAIQSRRRR"
     gene            561..1193
                     /gene="pth"
                     /locus_tag="P5516_03150"
     CDS             561..1193
                     /gene="pth"
                     /locus_tag="P5516_03150"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HRT91466.1"
                     /translation="MSLLKIFYKKTSAIKESTEAVYLLAGLGNPGREYRETRHNIGFM
                     MIDSFAARHNIKLNKVQNKAIVGVGRVNGVRVILAKPQTYMNQSGQAIAGLVRYYKVS
                     LENLIVGHDDVDLPFQTIRIRPAGGSAGQKGMTSIIEQLGTQNFARLRMGIGRPPGQM
                     DAAAYVLQTFTKAEKEMLGSFLDRAVEALQCYLNEGLETAMNRFNPTEKD"
     gene            1195..4575
                     /gene="mfd"
                     /locus_tag="P5516_03155"
     CDS             1195..4575
                     /gene="mfd"
                     /locus_tag="P5516_03155"
                     /inference="COORDINATES: protein motif:HMM:TIGR00580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HRT91467.1"
                     /translation="MLSTLRQQAAYLQCLDSIKVGTQPASLGLLRSARLPVVAALCID
                     LQVPVLLLTDRADRASMLFDELRFWLPDFPRLYFPEPTPMFYEKAAWGSLTRRDRLQT
                     MAVLVNYFKLGSQKPDKPCVVIAPLRAVMTRTLPRRDYIIASKVLKINGHANPDTLLR
                     EWARLGYENTEVVVDHGQFSKRGGILDIWTPAEPYPVRLEFFGEEIDTLRFFDPATQR
                     TVSNIDQIQITPAREFIPDGDLKFSGEDGFVDEFQIPMIHPLSSTILDYLPPDALILV
                     DSLSNIQSFGEEIEEQALKNRAEVVREGIIPADFPVPYISWSEVLEMISNRRWMELLR
                     PNNEDMGSLGSKFSPGMRFAGQISHFVNYLEACCREGDEMVIVTRQLSRLKEIWEEQS
                     DACAHSGNLKFIESALGEGWDLELDSGKTLHCITDGEIFGWERPQPRARLTARYLSPE
                     ADYADLKKGDWVVHVDYGIGRFAGLVSRILDGMEKEFLLVEYENGDQIYVPIHQADRI
                     SRYIGTGGEPPQPSSLNGHEWNQTKQVVREAVAQVASELLELYAKRQSAVGHAYKPDT
                     AWQQELEASFPYIETEDQMKAILSVKQDMETPKPMDRLLCGDVGYGKTEVALRAAFKA
                     VMDGKQVAMLVPTTVLAQQHFETFRQRLSPYPVSVEMLSRFRTPKEQEEIVLRIMLGQ
                     VDIVIGTHRLLTSDVVFKDLGLVVIDEEQRFGVTHKEHFKKLRTEVDVLTLTATPIPR
                     TLYMALTGVRDISTINTPPAERLPIVTHIGPYSPRIVRQAVLREIGRGGQVFFVHNRV
                     QTIEAMKAHLESLVPEARVSIAHGQMREEELSRVMTRFTKGEVDVLLCTSIIESGLDI
                     PNANTLIVDRGDAFGLAQLYQLRGRVGRGGQRAFAYFFKHRKKAPTPEGQERLETIAE
                     NIQLGAGYSIAMRDLEMRGAGEMLGTRQSGYIASVGFHLYTRMLAQAVRELRLEQGQN
                     EGESENAIPSSGYIPVSVDLPISVGIPVDYVPDQALRLQLYRRLAHLEEVSELDALAA
                     EFRDRFGQPVQQVKDLFFQLRVKLLAAKAGLTSINVEADQLVLRFPQLPEGAVKRELA
                     PLGYQTRIGKNAYWMKFSPEENEWRENLLKVLGAIIAS"
     gene            4527..5258
                     /locus_tag="P5516_03160"
     CDS             4527..5258
                     /locus_tag="P5516_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT91468.1"
                     /translation="MAGKLAEGSGCYNRFMTTILIIEDEVTLARILQSYLEREGYRVL
                     SAQRGDTGLDLCLSENVDLVLLDLNLPGMDGVDVATEVLREKDIPIIMVTARVEEIDR
                     LKGLELGADDYITKPFSPREVVARVKTVLRRVNRSRVEEDRFLIPPFEIDIDAHLVKK
                     AGQEIDLTPSEFAILVTLARHPGRVFSRLQLLEATQGQSFEGYERTVDAHIKNLRAKI
                     EEDARKPAFIKTVFGVGYKFYREDE"
     gene            5262..6632
                     /locus_tag="P5516_03165"
     CDS             5262..6632
                     /locus_tag="P5516_03165"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT91469.1"
                     /translation="MKFSLQQKMILAFSVVVIVSIGGVVIFANLDSQRQVNRYLTRGG
                     QYGVMELVEALEDYYEYKGTWSMVGEVADAYSQRHGRKNAHGKASELTLTDQNHFIVW
                     SNAGQVKGEKLTGEMTSRSLEIHSGGQTVGYLVVENGVLQTPDDIAPFVNRLRRAIFL
                     AGGFAILLAIGLAVLVSKYLLEPIKVITSASTQLSTGDFSTRVDIKGNDEIAMLAKTF
                     NQLARNLELAEERKQSLTADAAHELRTPIAVQKAQLEGMLDGVLPLTQKNVLIALQQT
                     NFLSRLVEDLRLLAMADAGEVRLEMRILNIVNLVQQVVAGFQAQAALEGTNLVTVFRP
                     DGEELWIHTDVDRVSQILQNLISNALRYGQKGGRILISTRKESAKLVISVQDDGSGIP
                     STALPHLFERFYRHERARTRENGGSGLGLAISKKLALLLGGDLSGANSPEGGAVFSLE
                     LPIRNT"
     gene            6690..8735
                     /gene="uvrB"
                     /locus_tag="P5516_03170"
     CDS             6690..8735
                     /gene="uvrB"
                     /locus_tag="P5516_03170"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HRT91470.1"
                     /translation="MQFHLQAPYQATGDQPEAIKQLVKGIRQGMRNQVLLGATGTGKT
                     FTIAKVIQELQMPALVMAHNKTLAAQLYAEFREFFPQNAVEYFVSYYDYYQPEAYVPR
                     HDLYIEKETDINDEIERLRLAATTALMSRKDVIIVASVSCIYGLGNPEEYSNVVVHLA
                     VGEIFRRNALLRRLIESQYQRNDLELRPGAFRVRGDTMEIFPAYEEKQAVRITFFGDE
                     VEKIVEFNPMTGEIIAEPSAIDIYPAKHYIASEARLKVALDDIEKELNDQIHYFKANG
                     RVLEAQRIEQRCRYDLEMLREVGYCSGIENYSRHLDQRPAGSPPWTLMDYLPSEYLLV
                     LDESHMTVPQIRGMYNGDQSRKSTLVEYGFRLPSALDNRPLTFKEFERVMGYTIYTSA
                     TPADFELSRSDQIVEQIIRPTGLVDPEVEVRPTKGQVDDLYGEIKKRVEQGERVLVTT
                     LTKRMAEDLSDYLLELGLKVNYLHSEVETLERVGILRDLRMGVFDVIVGINLLREGLD
                     LPEVSLVAILDADKEGFLRSATALIQTIGRAARNVNGKVIMYADTITGAMKTAIDETN
                     RRRKKQMDYNKANGIEPFTVVKAVHDLTERLSASSVIAEAKGRYSAGEEKPRMPISDL
                     KRMILELEKQMKQAASELQFEQAAVIRDRLIDLKTYLAEESDLPPWKKARLMAGEIE"
     gene            complement(8760..9047)
                     /locus_tag="P5516_03175"
     CDS             complement(8760..9047)
                     /locus_tag="P5516_03175"
                     /inference="COORDINATES: protein motif:HMM:NF012911.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HRT91471.1"
                     /translation="MAEIKCIHLLVEGTVQGVGFRVFVQQQANALALTGWVRNLMDDK
                     VEILAQGDENKLTRLLATVRVGPPMAHVTQVTVEWSNPVVFYKRFSIAPSG"
     gene            complement(9134..10183)
                     /locus_tag="P5516_03180"
     CDS             complement(9134..10183)
                     /locus_tag="P5516_03180"
                     /inference="COORDINATES: protein motif:HMM:NF025030.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HRT91472.1"
                     /translation="MNKESGEPIPFSSVLSALQNASVPFPARYLRSFSDLPPANLKVL
                     LNCWPSIPARRKVSLLEDLEAVLEEDTLVNFDNLARALLADPDPHVRTCALRLLWECE
                     EKSILPILVDISIKDEDETTRETAISLLGKFVLLGELDSLKDSAKEGLNQHLVNIVRG
                     KDKTRVKQRALESLGYSSHPAVPDLIRNAYQTNDLHWVTSAICAMGRSADDAWGPQVV
                     AMLTSPEPEIQFEAIRAAGELELRTAREPLLALLEEETEDEEMRLALIWALSQIGGDA
                     VREKLNELLENPSNEDEAEWLEKAMENLDISTPGDMNLLDFSPGDEEDDEEEDFLGDL
                     ELDDFNAEDEEEEDE"
     gene            complement(10191..11285)
                     /locus_tag="P5516_03185"
     CDS             complement(10191..11285)
                     /locus_tag="P5516_03185"
                     /inference="COORDINATES: protein motif:HMM:NF014244.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HRT91473.1"
                     /translation="MNAIGSTAITILEFILAFGALVLLHELGHFFAARLSKIEVEEFG
                     LGYPPKMFRLFRAGGTDFTINWIPFGGFCRMKGENGEVSEPGSFLAARPSRRLLTLLG
                     GPLTNLLVGMLIISFLFTRIGSPDPSSVMVAAVTDETPAAAAGILPGDLIQSVNNVQI
                     TSMDALSKVISSHLGQEVEIVLLREGVALTIKVTPRAEWPEKQGPLGITIMNPSQKVS
                     FIQALPRAFIATLDQGYQLLMVPVRLISGQIAPSEARMVSVKGIYDIYAQVQTLDKQE
                     AVENPAMAGLNTLYFFAIISIALGFTNLLPIPALDGGRILFLLPELFFKKKIPPALEG
                     KVHMIFYALLLTLMVLLVINDIVNPVIIPK"
     gene            complement(11636..12673)
                     /locus_tag="P5516_03190"
     CDS             complement(11636..12673)
                     /locus_tag="P5516_03190"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91474.1"
                     /translation="MDHIIDKNEKAEPFLQQDIRERGVLLSIRDLKIWFELRKFGFGH
                     AGYVHAVDGVSFDLHNGESIAVVGESGCGKSTLMKGILGLHRPTEGEIFFRGESIGEM
                     KSKEIKQYRSHIGYIQQDPYGAMAPFMSVQRILTEPLSINGIKDHQQIQDMVHKALEE
                     VKVFPVEDYLNKFPHMLSGGQQQRIVIARSMILNPNFLVADEPVSMLDASVRVEILRL
                     LRSLQESRNLAVIYITHDLSTVSYFSRRIFVMYAGQIVEKALVQDLLNNAGHPYTQAL
                     LTGTSDPDGANAHKLKEVPPGEPPNLINPPKGCRFHPRCPKIIRGLCEVEAPPEFEPE
                     PEHSVACWLLR"
     gene            complement(12688..13647)
                     /locus_tag="P5516_03195"
     CDS             complement(12688..13647)
                     /locus_tag="P5516_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91475.1"
                     /translation="MANSPTLLKIEDLVLYFKTMRGPVQAVDHIDLDLKNNEAIVILG
                     ESGCGKSSLAKAILRLLPRNIDKYSGKVLLNGTDTMQYSDEEFRRKVRWRSISMVPQA
                     AMNSLNPVLKVGDQVTEPFIIHTGASKTEAIAQAKKMFAHVGVPNSFLDRYAFELSGG
                     MRQRSAIAMALVTMPDLIILDEPTSALDVLTQANIFNVLKTLKKDLEISFILITHDIA
                     TSSELADRVAVMYAGQIVEVCDATRFFDKPFHPYSIALMDSVPRLRQVKEPDFITGQP
                     PSLINPPTGCRFKDRCHKRFEKCEEEPPAVTIENHIVKCWLYQ"
     gene            complement(13647..14975)
                     /locus_tag="P5516_03200"
     CDS             complement(13647..14975)
                     /locus_tag="P5516_03200"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91476.1"
                     /translation="MNSIKRTFNELFKYPSAVAGLIIILLLIALSIYTLIALPYDRAI
                     SLWRGESKDWYASPKYAMPAWINSFRKNKLPTTIILKTDDPSVVKTEEKATVIYEMPF
                     VFTADAFPSEINVFFKSTYTSSPPFVGLSIITPDEREIKLGNFKVERSLAYYLSQDQK
                     LVKKYKDIPIQEALLMQPDSKPRKPLKGTYKLIIEGVTFDEGSTVSAEAVFYGKVSGI
                     AGTDHKRRDLSIALLWGTPIALMFGLVAAVGTSLTQLIISAISTWFGGWIDVLIQRIT
                     NVNMVLPFLPILIMIGTFYSRSIFVILTCVILLSIFGAGILTYRAMFMQIKESPYIEA
                     SRSYGASNSRIIFKYMVPRLIPMLIPGFVSLIPSYVFLESSLAILGLGDPTIPTWGKV
                     IDESYNSGALFNGYYYWVLEPSFLLVVTGLAFAVVGYALDRVFNPRLRGQ"
     gene            complement(14972..16120)
                     /locus_tag="P5516_03205"
     CDS             complement(14972..16120)
                     /locus_tag="P5516_03205"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91477.1"
                     /translation="MVDASPTIDSFNSLEQEVIKEKRSSTALRILKYTASRMVMMVIT
                     VTIGVYLTVLIANMGGYVDTIRKAQIREQVGLIVANDPVLRKLSPEVRNIRIAEMVRD
                     QESIYGLDKPFLVRSFLYLKQALTLDLGRAMSMTSNSGSSSVRNIIIERLPPTLLLFG
                     TSDLVLFFLALMIALSLSRHYGSVMDKIVIALTPLSSAPGWFYGIFLILIFAAVLRVL
                     PFGGMVDAPPPKETMAYILSLLRHMLLPVAAITISSIFLSVFSWRTFFLIYSSEDYVE
                     MAKAKGLTERDVTQKYILRPTLPTIITNFALLVIGMWVGAPIFETVFQWPGLGRALYQ
                     AIGLFDTPVIVAEAVIFSYLLGITVFLLEIVYALVDPRVQVGNEGPRA"
     gene            complement(16196..19066)
                     /locus_tag="P5516_03210"
     CDS             complement(16196..19066)
                     /locus_tag="P5516_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT91478.1"
                     /translation="MRKLYAILGVLIAATMVLSACAKPTAAPTAAPEVPAATEAPVVE
                     TAIPHNGKGAWLDKVIFTAVADADSVVAQLQAGAIDIYPVSVEDPEVFAKVKADENLG
                     YATVYGSSNQLMVNVVKCDDGSLNPFTDMEFREAMNWAFDRDYVVQEFFGGLAIPKFT
                     SFTGAFPDYARYADVMAAITSTYAYDMEKAQAAVDARMTALGATKNASGVWEFNGAPV
                     TIKVVIRTEDQRLGIGQYFASQLEALGFKVERLEKTRTEASPIVWSATPELCEWHVYT
                     GGWISTAISRDDGYQIPQFNTGLVQTTLPIFSKYDPSPEFDVINQKLLYNDFTSMEER
                     DQLIRDGLNLAMKESWWGVWVNDNTAISPYRKPLEGAYDLAGGFASAPLWPYTMRWAD
                     KVGGVVRVAQSGILVQPFNPINGSNWTDDSMVYRGIMDWGLVPNPFTGLNMPKLVEKA
                     ELVAKTGTPIIENGEDWFKLSFQDAVEVPEDAWADWDPVNEKWLTVAEMKVKVEEAKA
                     KAAEIEAVKAEVEAKRAELVAAFDTTNMTVETMAAVTKEFTDFAAEKYGLPWDASAFV
                     DAELPGEVEWIDGNLAADAVADRQGELDWWLGYVAESTDPSVSVVDLAGRDYSTAVTK
                     SVVIYRPELWDTTWHDGSPFTIGDVVYGMIMNFAPGKEGSPIFDSTMKGPVDAYLSHF
                     RGVKITSVDPLTIETYDNAFALDAENSVSAWYPSFSYGPVAWHDLTPAVLAESDEKIA
                     FSKAKATQLGVDQTSLIAGETLAIQKKYLDEAAAAGTIPFAPTLSEYITADEAAARYA
                     NLLGWFQDKGHLMIGTGVFYVDEVYPTEGTVTLSRYADYIFPSDEFSGFGAPMIADAE
                     IDGPLAITKGEAADFTVAVTYDDEAYPSAKLNFVSYMLFDATGKLVEKGNATMTEEGL
                     YAVSLSAETTGKLEVGGAKLLTAVSSKVVSIPTFSEYEFVVQ"
     gene            complement(19244..20224)
                     /gene="mvaD"
                     /locus_tag="P5516_03215"
     CDS             complement(19244..20224)
                     /gene="mvaD"
                     /locus_tag="P5516_03215"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: protein motif:HMM:TIGR01240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004163 - diphosphomevalonate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HRT91479.1"
                     /translation="MPMIFSTATAVSHPNIAFIKYWGNTDDALRLPQNGSISMNLAEL
                     TTRTMVTFDSSLPRDIFDLNGVRQSGPSLERVSQHLNLIRGIRGFSVPAHILSESNFP
                     VGAGLASSAAAFAALTVAAVRTAGMEMSEKDLSRLARRGSGSAARSIPPGYVEWLPGS
                     SDIDSYAVSIAPPEHWPLTDCIAIVEDAHKKTGSSEGHLLARTSPLQSARVADAGRRI
                     DLCRNAIQKKDFDLLSQLIELDSNLLHAVAFTSNPPITYWLPQTLVLMQAVKDWRRAG
                     IPCAFTIDAGPNVHVICESSATSIVKERLGAIPGVSEVLTSGAGGGTVLI"
     gene            20513..21829
                     /locus_tag="P5516_03220"
     CDS             20513..21829
                     /locus_tag="P5516_03220"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRT91480.1"
                     /translation="MMEKVIRFNLTIEPGQQGSYFTVPFEVPQGIESLELTYEYPRRL
                     KRKEEQPGEIFTATPETNIVDLGLIAPDGRQVGASGSEKTRILVSEQYATPGYGARTV
                     SAGRWQVIVGAYQVAPEGVEVLYEITLKEKSLRLLKGDTHVHSIASDGVHTFEELALK
                     AKANGLDFLAVTDHNVFLPAASIPYVPGITMIPGVEWTHYQGHANFLGIDAPYDEPFF
                     TNSVEEARSRFTCAHERGALITINHPFEGNSRFLFDFRQLPFDCLEVWNGPMRESNLK
                     AVGLWQQMLSSGMRVAITGGSDYHRDTPFIFLGGPTMGVYALSAGMSDILSALRKGHS
                     YITFAPDGPSIEMRAGEGIMGDQVDWQEEKELRVRLQGLQAGDVVLVVTAQENAAVFT
                     ATSEGELDFAYEMREPGFARVEVLRAFLPGLPLLPALMSNPIYFKA"
     gene            complement(21819..22967)
                     /locus_tag="P5516_03225"
     CDS             complement(21819..22967)
                     /locus_tag="P5516_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91481.1"
                     /translation="MKSGFTLRNWTEKVEALPSWVLLVVASVLYIPLVFFGYGSDSDS
                     LGVVRTGQYFIATFDYLPSRLPGFFVHEFFVFFLNQLGGSVLTNLGSVCMSLVMLHSF
                     RQVCRHYAVPHPTLLSAVLMVQPFFWVSAASTIDYLWALGFCFLGFNLLLHQRNLPAA
                     ISLALSVGCRLSTVLPVGLFLCFLFLTSVPQRRWILLTSFCAALMSIVFFLPPLDFLE
                     WDFRRWLVLSTGDPALWTPVLRVGRFLYKNLMFFSLPVVLWACFLCLSALFHKQKPTL
                     PRPDSLSWLSLAVILSVEIMFLRIPIEMEYLLPLLPFALILIGKALPNHPGQLWLLLA
                     LTLLANFLWINPARSLTPNQTSEVIYGLWLEPGYLLQDIATRLSLLKP"
     gene            complement(23015..24139)
                     /gene="alr"
                     /locus_tag="P5516_03230"
     CDS             complement(23015..24139)
                     /gene="alr"
                     /locus_tag="P5516_03230"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HRT91482.1"
                     /translation="MLYQTHAQIHLANIRHNIEGIRHAIGQQCKILIAVKANAYGHGA
                     VEVSRMAQNIGVNWLGVATVPEGMELREAGIHLPILKFSPAFPEEMECAVENELTLTV
                     CDKANIQALQRVCQRLKLTARVHLKIDSGMGRIGVTPGEAPELAKFIHSRCSNLLLEG
                     VFTHLPVSDSMGNDYTQAQIDRFKSVVLTIEDAIGAKVPLVHCANSGGVLDYPQSWMD
                     MVRPGIMIYGFYPGEPSSHRVDLKPGLSLRTRVSFIKKVEKGTSIGYGRTWFAPQDTW
                     IATIPAGYADGFNRLFSNTGRVLIDGVSYPVVGRVCMDQSMINLGAETHVKVGDEVVL
                     IGRSGAEEISAYEWAEKLNTITYEVTCQINQRVPRVFDPY"
     gene            complement(24151..25242)
                     /locus_tag="P5516_03235"
     CDS             complement(24151..25242)
                     /locus_tag="P5516_03235"
                     /inference="COORDINATES: protein motif:HMM:NF013345.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine/ornithine racemase family PLP-dependent
                     enzyme"
                     /protein_id="HRT91483.1"
                     /translation="MSKLFTPRLELFPERIAENARAIIHLCHDHGIQVACVGKVVCAH
                     PVIMRALEESGADLLADSRLENLRSLRQNGTTLPLMLLRIPTPSQVEEVIHAADISLV
                     SDLETIKKISAAAMAAHSTHKVILMVDLGDLREGIWPSRLDEVLRAIIRLPGIEVNGL
                     GCNLACYGGVIPTTEKMQQLIDLRDQSETSLGIRLPVLCAGNSANLPLLLADKMPLQI
                     NMLRIGETILLGRNVLDRSPFPGTRQDTARLVAEIIELERKPSVPIGERGQDAFGGTP
                     EFVDRGVRLRAICNIGRQDVVIDNLTPEDPGIIVLGGSSDHLLLDVQDAQQPLAVGDE
                     VAFYPGYGALLAASTSPYVRKTMINGISS"
     gene            complement(25239..26576)
                     /locus_tag="P5516_03240"
     CDS             complement(25239..26576)
                     /locus_tag="P5516_03240"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRT91484.1"
                     /translation="MPEPICILTIEVGSTITKVNGFYRSQHGALVQAAQGFAPTSITQ
                     GDVMPGVELAMQQVKDGANPLDPEVEIFINSSAAGGLRMTVHGLTYNMTARAAREAAL
                     GAGAIVKKVTAGLLDEFELSEIRQIHPNIILLAGGVDYGEKETVLENARRIANLGLQV
                     PVVYAGNRVLRQPITEIFKTQDIDLLIADNVFPEVDVLNIEPLRRIIHEVFNRHIIHA
                     PGMARLGELTRHGILPTPGAVLLASELFAEEVGDCVVFDVGGATTDVHSVTDGSQEWS
                     LKLLEPEPRAKRTVEGDLGVYVNAKHIIEIVQDWESEDRLADLQAIPVTDKQLELTHW
                     LCTKAVEISARRHAAQVTDLYTPSGRKQVVKGKDLTAVKRVIGTGGALTRVTGGVEIL
                     QRVCTGAGKYLLPQPGTPILLDDAYRFSALGTIAQKYPDEVKSTFVDWVRKEI"
     gene            complement(26598..28811)
                     /gene="oraE"
                     /locus_tag="P5516_03245"
     CDS             complement(26598..28811)
                     /gene="oraE"
                     /locus_tag="P5516_03245"
                     /EC_number="5.4.3.5"
                     /inference="COORDINATES: protein motif:HMM:NF040743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ornithine 4,5-aminomutase subunit OraE"
                     /protein_id="HRT91485.1"
                     /translation="MTNTPEKLDPNQKMDIEHILEGLEHYHPRRKGWTWREIPQEGVE
                     MGPFHYHDMSKPLKNSIGLPASKYFDHIDPQPSCVVTTEIASGRFEDDIRRMRMAAWH
                     GADHLMVIRTTGQSHIDGLMEGTPEGVGGVPITRKQIRASRKACDQIEEEVGRPINFH
                     SYVSGVAGPEVAVLFAEEGINGAHQDPQYNVLYRNVNMYRSFVDAAEAKKVMNGARIF
                     QIDGAHNANATAKAGWLVMPELMVQHGINCSFSVAVGMDKDLIGLSTVPPNAPPAPKL
                     WYDLPYAVALRDFFSDFKMRAQQNTRYIEADIEEAIRTHTIDTLISMLTSADIQSTIT
                     PDEGRNVPWHYNNIRGVQTVKQTWAALDGIKDLVALKTNGPMKEMVRDLKVRAVAFLE
                     EVIETGGYFAAVEKGFFVDSAKYPERNGDGIARDGKGGVAADTIVERDADYFAPVCDH
                     FGYNNVPAELQRPCDAIQGCTLCKPEKIVYIDELDPEDNCEKRLQETYPYRKGGKIKP
                     EAEWAGDGTILTQFFVPLPEDYARVYGVEMAKKLGLIDPEVINIQVMHPAEGCFVEVK
                     GKVNFELNLTDLKIPAKEIMLEEETLRQVVKKVGLTVVAGTVGEDEHSVGLREILDIK
                     HGGIEKYGVKYHYLGTSVPVSKLVDAAIETGAQAILISTIISHNDVHRSQMKKLADLC
                     VERGVRDRLILIAGGTQVNAEMAAETGLDATFGRGTKGIHVVNAMVKNLQKRGLA"
     gene            complement(28823..29173)
                     /locus_tag="P5516_03250"
     CDS             complement(28823..29173)
                     /locus_tag="P5516_03250"
                     /inference="COORDINATES: protein motif:HMM:NF027866.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine aminomutase subunit alpha"
                     /protein_id="HRT91486.1"
                     /translation="MSEDRIKRFTERQKELNKLSDEELKNQFWQLCDRLVEPIVELAR
                     THTSPSIERSVLLRMGIDSITAHGVVDRVLEAGLLGKGAGHVVLKVAEKQGVSIPTAA
                     ALINEKKEILSGLF"
     gene            complement(29166..30611)
                     /gene="ortB"
                     /locus_tag="P5516_03255"
     CDS             complement(29166..30611)
                     /gene="ortB"
                     /locus_tag="P5516_03255"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: protein motif:HMM:NF040741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtB"
                     /protein_id="HRT91487.1"
                     /translation="MSQRDVSYAAVMARKNDIMKSSMGMDYEKYISSPVAFDYEAMMR
                     DTGYTMDEIFRIQQETKVGNTPLYELRNLTEAVRQISPPGKGATILVKDEAANASGSF
                     KARRASISAYEAAHKGYAGMVAATSGNYGAAVASQAAQRKLKCIIVQEVYDSRGIGQP
                     EIVEKGRACEAYGAEVLKLTVGPELFYMLLRTLEETGFFNASLYTPFGIRGVESLGVE
                     IAEQTRARYHRDPDAVIITHAGGGNLTGTARGLLHAGSCATRIVACSVNLQGLHMASD
                     HDFNRKSFTTGHTGFGVPFATWPDRVDVPRNAARSLRYMDEYQLVTQGEVFYITELLT
                     KIEGLERGPAGNISLTAAVALARQMEREQVVVVQETEYTGAGKHQNSQLAFARENGIE
                     IRRGDPLDDIPGKTIIIPEKLEQVQGKSQDIDRLRLEYLQNAGKQLAPSLWSESDISF
                     LALDIKKTPDWIRENFLSLPQNNENLEKTNV"
     gene            complement(30608..30892)
                     /gene="ortA"
                     /locus_tag="P5516_03260"
     CDS             complement(30608..30892)
                     /gene="ortA"
                     /locus_tag="P5516_03260"
                     /EC_number="2.3.1.263"
                     /inference="COORDINATES: protein motif:HMM:NF040739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-oxopentanoate thiolase subunit OrtA"
                     /protein_id="HRT91488.1"
                     /translation="MNIIPKGTWVEIEQVVLKPEERAASLPEDTKKVPYLLWVSGFLL
                     EDAKIGQKVRVKTIIGRELSGELKVVNPSYHHSFGTVVPELLTIGTEYEK"
     gene            complement(30924..31964)
                     /gene="ord"
                     /locus_tag="P5516_03265"
     CDS             complement(30924..31964)
                     /gene="ord"
                     /locus_tag="P5516_03265"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF040740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4-diaminopentanoate dehydrogenase"
                     /protein_id="HRT91489.1"
                     /translation="MKPIRVLQWGLGAMGSGMTRLILEKSGLQIVAAVDSRTDYAGKD
                     LGEVLKLDRSLGVTVTSNPDSVLNKERVDIVVIATTSWLSEQMHDLKKIIQAGINCIS
                     IAEEMSDPYAQNPELAAQLDVLCKANQVTLLGTGVNPGFVLDLLVVMLSGGSHTVERI
                     EASRVNDLSPYGPTVMKSQGVGITPAAFKAGIEDGSIVGHVGFPESIHMISDALGLGV
                     DRIEQAREPIISNVYRETPHVKVQPGMVAGCAHTGIGYRGDKEVIRLIHPQQIHPQLE
                     GQDTGDYIHIFGKPEINMAIKPEIAGGIATMGLAVNMIPHVVAATAGLKRMIDLPAPA
                     ALMGETAYRRQV"
     gene            complement(32076..33191)
                     /locus_tag="P5516_03270"
     CDS             complement(32076..33191)
                     /locus_tag="P5516_03270"
                     /inference="COORDINATES: protein motif:HMM:NF013347.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HRT91490.1"
                     /translation="MILAQELKALNLTILEPEPATAASKNRVLPDDPGGWLLSGSLED
                     VYRSNLYLRTASRVSVRLGEFYAAAFSELRKKASRLEWERYLSPGQPVHLSVTCHKSR
                     LYHSDAVAERVVGAINDRFSNSKKETQPSKNGQLVLVRLVNDLCTISIDSSGALLHKR
                     GYRLESTKAPLRETLAAALLLLSGWSGEMTLIDPFCGSGTIPIEAALLARNIPPGVAR
                     DFAFMHWPGFVPHEWERVLHSARDGIIRQDSHIFGYDRDQGAVQSAISNASRAGLNEV
                     IHFQRQSISELSPPRSNPGWLITNPPYGVRISGSKDLRNLYARFGNLLTTAFSQWQAG
                     ILCYDKKLTANLGLPPQQASYHFTNGGLPVDFNVFAF"
     gene            complement(33245..35083)
                     /locus_tag="P5516_03275"
     CDS             complement(33245..35083)
                     /locus_tag="P5516_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR03904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgiQ family radical SAM protein"
                     /protein_id="HRT91491.1"
                     /translation="MIKAAYRLLFFTSRLRCYTQFVRQQGTIVFIPTTREELVHLGWE
                     QPDVILVSGDSYIDTPYSGLAVIGKILISQGYRVAIIAQPDMSGSEDIRRLGEPLLFW
                     GVSAGTVDSMVANYTATLRKRRTDDFTPGGANIRRPDRAVIAYTNLIKRYHKNTVPIV
                     LGGIEASLRRITHYDYWSDRLRAPILFDSKADYLLYGMADGSIVELALALRQKRSPRE
                     IRGLCYLAAEAPSTYLELPSFEEVSADPDAFIHMFDLFYRNNDPLTARGLVQKKGDRY
                     LVQNPPPPTPSTQALDTIYDLGFERLHHPYYELQGEVKALQTIQFSIPTHRGCYGECN
                     FCAIAVHEGRTVVSRSEASILKEAEKLSHHPDFKGMITDLSGPTANMYGFECRKKLVS
                     GACEDKRCVFPNICPALPVDHSRQVALLRKLRNLPGVKKVSVGSGIRYDLVLADRRSG
                     MQYLKDITCYHTSGQLKVAPEHTDPVVLNLMGKPSHESLRLFREKFLEFSRQAGKKQF
                     LSYYLIAAYPGSSEREMKKLREFVTKELRVQPEQVQIFTPTPSTYASVMYFTEKDPFS
                     GKKLFVEKNLHRKALQKQIITNPGRSKTGGTQKPFEKHPLSRNRKP"
     gene            complement(35080..35592)
                     /locus_tag="P5516_03280"
     CDS             complement(35080..35592)
                     /locus_tag="P5516_03280"
                     /inference="COORDINATES: protein motif:HMM:NF012478.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRT91492.1"
                     /translation="MNPSNDLRKVSKDLVVSMAYELKIEGELIDQATEDDPLQFIQGH
                     GHIITGLESAIEGMNIGESKHVSVKPEDGYGVYSEEDIIEIPRSEFPEDFPLEVDMEV
                     TVEDDEGIESSAFIEEITADSVTLDFNHPLAGRDLEFEVKIIALRLPTSEELNHDHVH
                     LDEHEQLEDE"
     gene            complement(35693..36055)
                     /locus_tag="P5516_03285"
     CDS             complement(35693..36055)
                     /locus_tag="P5516_03285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91493.1"
                     /translation="MIRKIERKFHIKTNGYFLVDNFSASSIETWLGLENPSSPSQPPL
                     TPLERQTILSHGQKVISQFCAQISQNGIDQVINQIHWADLLPEHFSTNVSTELWLQAV
                     NKLASFEKIGSCEVFIGQ"
     gene            complement(36082..36390)
                     /locus_tag="P5516_03290"
     CDS             complement(36082..36390)
                     /locus_tag="P5516_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91494.1"
                     /translation="MKKIALALIALLFLVSVYFGFQAAAHLDGHLKKTAPNNSLEPIS
                     AQQNYLIFQVSELGGDSPMLVSVWGLFVRDAPPLTWRLSRSTHPQIQSKKTTHSPFSV
                     "
     gene            complement(36485..36703)
                     /locus_tag="P5516_03295"
     CDS             complement(36485..36703)
                     /locus_tag="P5516_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91495.1"
                     /translation="MIAEIINSIELSFWNLIITLLTKSSLLRTIVRSTYLASQDKGMM
                     RKIAIVLLVFCVGFASGLLIYSVTLLVM"
     gene            complement(36712..36930)
                     /locus_tag="P5516_03300"
     CDS             complement(36712..36930)
                     /locus_tag="P5516_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91496.1"
                     /translation="MDARIKNAVCTSVYRQFPELKGAPPRVKALPADKYQLIFHGQAK
                     TADGKTLSRTVRVVSDENGKILKLTTSR"
     gene            complement(36941..38107)
                     /gene="ftsZ"
                     /locus_tag="P5516_03305"
     CDS             complement(36941..38107)
                     /gene="ftsZ"
                     /locus_tag="P5516_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRT91497.1"
                     /translation="MDYSTFHSHVARTPIKANPPVLKVIGLGGGGQNAVDRMIELGLE
                     GVQFIAANSDAQVLQHSQADLKIQLGRKITRGLGAGGDPAVGQAAAEESIAELTSALT
                     GADMVFLTAGMGGGTGTGAIPVAARVAKSVGAVVVAVVSLPFSFEIGRRQTNARNGLL
                     MLQQHTDTLITVPNDRLLQVAPRNLPIEMAFRLADDILRQGIQGISELITQPGLINVD
                     FSHIKNMMLNGGGSLLAIGTGKGENRVSDALEHALHHPMLESIDLEDASGIIANFTGG
                     NDLPFFEVTEALNTLQEKSGGMAEIVPGIICDDRMEDRVQVILVLTGIGTTALDPQRM
                     KRPVPVMTPAEDTDKETSLSPGNSYQENIPDVHPELAAAQSELDVPAFMRRKAR"
     gene            complement(38110..39453)
                     /locus_tag="P5516_03310"
     CDS             complement(38110..39453)
                     /locus_tag="P5516_03310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91498.1"
                     /translation="MILKSKKTRELVTGRVGIHYFPDTSHYTNKDLSNWLPVLQRLKC
                     AWLVLLSDTSRAIPEQFITALIKAGITPLVHIPLPLPNTPSPADLKAILAAYARWGVK
                     HIILFDRPNDMANWSAAGWVQQNLVERFVDRFLPLATATVKAEMMPIFPPLQPGGSYW
                     DLSFLKQSLQSIQRRGHSSLINQMGLASYAFTFNHELDYGAGGPQKWPKSLPYITDAQ
                     GQDQRGFFNFEWLQATARTVCDQDLPIFMLGAGIREPGQPYSPEVHAGICLSIMEMLK
                     ENSSKTAIPDYVISCSFYLLSAEPDSEEFACAWYKSPEDYLPVADLLMQAQVPGASNH
                     ANVPLEKADLLVEAESTPIKSFTGHPIDHYLLIPVYEWGVADFHLDVTRAFVQKHRPT
                     IGFSIREAALARRVTVIGGEQSFPETSLEELRRSGSVVERISGDGTSIATQLAER"
     gene            39553..40449
                     /locus_tag="P5516_03315"
     CDS             39553..40449
                     /locus_tag="P5516_03315"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HRT91499.1"
                     /translation="MILVTGGTGFIGRSLIQALVSSGEQVRVLLRPSKTSPNFPKGIS
                     VEVAVSSFSDQRSLRAALKGVDFLYHFAGAERQGLNANLNQIDVEGTATLMRAAKDTN
                     LKRVFYLSHHGADRGSAYPVMKAKGIAENWIMDSGIPYTILRTGAVFGPGDQFTIPLT
                     RLLRISPYFTLMPGDGSNLLQPIWIEDLVTCILLAAADDNKRNRVIPVGGIEYLTYLE
                     ILKLVMKQTGIQRKPLGVTPEFLRGLTLWIAQVYPKLPVSIFWLDYLAVDRITSLDSV
                     PREFGLIPARFKNKIDYLNKKI"
     gene            40456..41874
                     /locus_tag="P5516_03320"
     CDS             40456..41874
                     /locus_tag="P5516_03320"
                     /inference="COORDINATES: protein motif:HMM:NF015052.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HRT91500.1"
                     /translation="MPENRAFCIHAHFYQPPREDPFTGKIPEEAGAAPFANWNERILA
                     TCYQPNVEERNFSQLSFNLGPTLVEWMSQHAPDVLDRIVQEDALNATLYGTGNAIAQP
                     YHHTILPLASNEDKRTQVLWGIAAFNHVFVRAPLGMWLPETAVDYESLRVLASAGIQF
                     TMLAPWQVEVIEGESPYLVELGAGKSIIVFVYHGGLSSTVSFDAFATSNADAFAEFYL
                     KPEIDRFNPNQFLLIATDGELYGHHQPYREKFLSRLLDGSSNGIGLRATYPALWMKNH
                     QVTGRARIVENTSWSCFHGIDRWRTECDCTAGSTWKAGLREGLSLIAARIDDAFSEIC
                     AAHGLDPITARNDYIQVMLGMDTFEQWLSKTSGKEVSSIGKRKLATLFSAQRLRQQMF
                     SSCGWFFDELARIEPRNNLAYAAHAVVLMESLTSMDFHSVAQPALREAVSHTSGETAF
                     DFFIFAYQRFKNLQKTAQANVG"
     gene            complement(41834..45142)
                     /locus_tag="P5516_03325"
     CDS             complement(41834..45142)
                     /locus_tag="P5516_03325"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRT91501.1"
                     /translation="MGLIDEIFHHIFKLYKEQKNPQVLKQALAYLEQQSTPTALNAFL
                     ELFVREFPPSPVYQKQVDAHEYLLGNTDGVSNRELLIEELIMLWLSLENPALDSYREL
                     FAEKTFINDSRYALFISEINHFFALQPYFGPSNFPLTNMLRSPAIAVPDSITGQLEYI
                     REHWAMLLGDYLYRLLSSLDLIKEENKLGITGPGVTAIPVYDRDGFFQAGGSSEIEAF
                     SKDREWMPRLVLMAKNSYVWLDQLSKKYQREINRLDQIPLDELETLSRWGITGLWLIG
                     LWERSPASARIKKLCGNPEAISSAYSLHSYQIAADLGGENAYLALKENAARFGIRLAS
                     DMVPNHMGIDSDWVIHHPDRFLSLDYSPYPSYSFNGENLSSHPDVRIQIEDHYYDRTD
                     AAVVFKRQDLRNGQVDYVYHGNDGTSMPWNDTAQLNYLNPEVREAVIQTILEVAHRFP
                     IIRFDAAMTLAKKHYQRLWFPVPGSGGAIPSRSEHSLTAEEFNRLFPVEFWREVVDRV
                     AVEAPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLRNEDNAGYRRLMRNTLEFD
                     PEILKRYVNFMNNPDERTAVEQFGKSDKYFGICMLMATMPGLPMLGHGQIEGFSEKYG
                     MEYRKAYWDEPVDNDLVSRHEREIFPLLHQRRLFAGVDHFNLYDFFTNNGNVNENVYA
                     YTNYDAGNAALIVFNNRYEDTEGWVHTSVPQLRCYGQEKKLEQVSIAQALHLPADRNG
                     WLVFRDQISQLQYLRPLDEVLDRGFFFRLHAYHYQTLLDFQVVHADSAHDYASLYGLI
                     GRQGVPDIAQALNELTLKPVLEPFQVIVNQGYLGYLLQKYSTSPTLDSTEAREIQERI
                     GKFLQGVALYTGNSGELRSLQQETSSLFLTALMFSQELKKRVTRLSPSTSTFLDALEN
                     HLEQSPALRVTLLMLSLTSRLGKVASQEDFSEQSLAWLDEFRLNKTFIEVLQSMGVTS
                     QEIQNSALLLRIGITHQGWYNATKNEPMEEIIVRWFTDPQVQTLLRVNRYQDVLWYNR
                     ESFETLVWWLSLIALYQYIANPGADASTITEVVLNLNQLLGKLKKLEAKSGYQFDRLF
                     DFNQHSPGQSSVNS"
BASE COUNT        11169 a        11042 c        11972 g        10976 t
ORIGIN      
        1 ctcttgggga tgacaattac acccggattg tatatcactg cgcccacaac cggcgaggtg
       61 gtagaaggga ttgtagaaat ccggggcagt gtaccggaag aaggattttt atcagcagaa
      121 gttctgtacg cgtatgcgca aacgggtaaa gaaacctggt ttctaatcaa gcgactggac
      181 aaagtggttc aggatgatgt tctggctgcc tgggatacaa ccaccatcac cgacggcgtc
      241 taccatatca aacttgtggt aacaaaggtg ggggatcgtc gaaatgaggt ggtggttgag
      301 aacatacagg tttccaacta ctctcgtgct ccgggtcaga atgaaatggg tgtaaatacg
      361 cagacggaga aacgatcagc agcggttgag atcagagcac ctgtttcgac cgtgattgaa
      421 aaaaaggtca acccggcttc catccaacgg caggaaattc aacgaacgat cattattgcc
      481 attcttgctg tctgtgttgc gctgtcttca attgccatct attcagcgat acaatcccgt
      541 cgacggcgat aagagggaat atgtcattac tcaaaatatt ctacaaaaaa acctctgcta
      601 ttaaggaatc gacagaagcg gtatacctgc ttgccgggct tggaaacccg ggtagagagt
      661 accgcgaaac ccgacacaat atcggtttca tgatgattga ctcattcgca gcgcggcaca
      721 atatcaagtt gaataaagta cagaataaag ccatcgttgg cgtgggtcgc gtcaacggcg
      781 tcagggtaat cttagccaag ccgcaaacct atatgaatca gtcgggtcag gcgatcgctg
      841 gattggtacg gtactacaag gtttcgttgg agaacttaat tgttgggcat gatgatgtgg
      901 accttccttt ccagaccatc cgaatacgcc ctgctggcgg ttcagccggg caaaagggca
      961 tgacttcgat cattgaacaa ctgggaacac aaaattttgc ccgactgcgc atgggcatcg
     1021 gccgaccccc tggtcagatg gacgcagcgg catacgtgct gcagaccttc acgaaagcag
     1081 aaaaagagat gcttgggtca tttctggacc gggcagtgga ggcgcttcag tgttatttga
     1141 atgaagggtt ggagactgcc atgaatcgct tcaacccaac agagaaggat taaattgctt
     1201 agcacattac gccaacaggc ggcatacctg cagtgccttg attctatcaa ggtggggaca
     1261 cagccagcca gcctgggttt gctgcgttca gccaggctgc ctgtagtggc agccctgtgc
     1321 atcgatcttc aagtgccagt cctgctatta acggatagag cagatcgcgc ctccatgctg
     1381 tttgatgaat tacgcttttg gctgcctgac ttcccgcgcc tgtattttcc tgaacctaca
     1441 cccatgtttt acgaaaaagc cgcctggggc agcctgaccc ggcgggaccg gctgcaaacg
     1501 atggcggtac tggtgaatta ttttaaactg ggcagccaga aacccgataa accctgtgtg
     1561 gttatagccc cactgcgggc agtgatgacg cgaacgctgc cgaggcgcga ttatattatt
     1621 gcgagtaagg tattaaaaat caacggacat gccaatccag acacgttgtt gcgggaatgg
     1681 gcgcgcctgg ggtatgaaaa caccgaggtg gtcgttgacc acggtcagtt ttccaaacga
     1741 gggggtatcc tggacatctg gacgcctgct gaaccatacc cggtaagact ggagtttttt
     1801 ggcgaggaga tcgacacctt acgatttttt gacccggcca cacaacgaac tgtgagtaac
     1861 atcgatcaga tacaaattac ccctgcgaga gaattcatcc ctgacggcga cctgaagttt
     1921 tcgggtgagg acggttttgt tgatgagttc caaattccca tgattcatcc gctttcatcc
     1981 acgatcctgg attatttgcc accggatgcg ttgattttgg ttgatagcct ttcgaatatt
     2041 caaagttttg gcgaagaaat agaagagcag gctttaaaga atagggctga ggttgtgcga
     2101 gaagggatca ttcctgctga tttcccggtt ccgtatatat cctggtcaga agtgcttgaa
     2161 atgatctcca accggcgctg gatggagctg ttacgcccga acaatgaaga tatgggttct
     2221 cttggctcga aattttcacc cggtatgcgc tttgcggggc aaatcagtca cttcgtcaac
     2281 tatcttgaag cctgttgtcg tgaaggcgat gagatggtga ttgttaccag gcaactttca
     2341 cggttaaaag agatctggga agagcaaagc gacgcttgtg ctcattcagg aaatttgaaa
     2401 ttcatcgaat ctgcattggg tgaaggctgg gatctcgaac ttgatagcgg gaaaacacta
     2461 cattgcatta cagatggcga aatatttgga tgggaacgac ctcaacccag ggcgcggtta
     2521 accgcgcgtt atctctcccc ggaagcagat tatgctgacc ttaagaaggg tgactgggtg
     2581 gtgcatgttg attatggcat tggccggttt gccgggttgg tgagccgaat tctggatggt
     2641 atggagaaag aattcctgct ggtggagtac gaaaatggag atcaaattta cgttcctatc
     2701 caccaggcag atcggatcag ccgctacata ggtacgggtg gtgaaccacc tcagccctca
     2761 agtttaaacg gacatgagtg gaatcaaacc aagcaggttg tacgcgaagc ggtggcgcag
     2821 gttgccagtg aattgttaga actatacgcc aagcgccaat ctgcggttgg gcacgcttac
     2881 aagcctgata ccgcctggca gcaagaactg gaagcaagct ttccctacat tgaaacagaa
     2941 gaccaaatga aagcgatttt atctgtgaag caagacatgg aaacgccaaa gcctatggac
     3001 cgtttgcttt gcggcgatgt tgggtatggc aagacagaag tcgcattacg ggcggccttc
     3061 aaagcggtga tggacggaaa gcaggtagcg atgcttgttc caaccaccgt gcttgcacaa
     3121 caacattttg agaccttccg gcagaggctg tctccttacc cggtttctgt tgaaatgctt
     3181 tccaggtttc gaaccccaaa agagcaggaa gaaattgttt taaggatcat gctggggcag
     3241 gttgacattg taattggcac acaccgtctg cttaccagcg atgtggtttt taaagacctg
     3301 ggtttggtgg tcatcgatga ggagcagcgg tttggcgtta ctcataagga acattttaaa
     3361 aaattacgta ccgaagttga tgtactcacg cttactgcca caccgatccc gcgaaccctg
     3421 tatatggcgc tcactggagt gcgtgatata tccaccatta ataccccccc cgcagagcgc
     3481 ctgcccatcg taactcatat tggtccttat tcgccgcgga tcgttcggca ggcggtgcta
     3541 cgtgaaattg ggcggggcgg gcaggtgttt tttgtgcata accgcgtgca aacaattgag
     3601 gcgatgaaag ctcaccttga gagtttagtg ccagaagcgc gggtctcgat cgcacatggc
     3661 caaatgcgtg aagaggagct ttctcgcgtg atgacccgtt ttaccaaagg cgaggtggac
     3721 gtgttactgt gcacttcgat tatcgaatct ggcttggata tccccaacgc caatactttg
     3781 atcgtagacc ggggagacgc ctttgggctg gcgcagttgt atcagttgag gggcagggta
     3841 ggcaggggcg ggcaacgggc tttcgcttat tttttcaaac accgcaagaa agccccaacc
     3901 cctgaagggc aagaacgcct ggaaacaatc gcggaaaaca tccagttggg ggcgggctat
     3961 tccatagcca tgcgtgacct tgaaatgcgt ggagcgggtg aaatgctcgg tacccgtcaa
     4021 tcgggttata tcgcttcagt tggttttcac ctctacacca ggatgctggc acaggcagta
     4081 agggagctac gccttgaaca ggggcaaaat gaaggagaat ctgaaaacgc aattccttcc
     4141 tccgggtata tccccgtcag tgtggatctg ccgattagcg tgggtattcc tgttgattac
     4201 gttccggatc aggctttacg attgcagtta taccggcgac tggctcacct ggaggaagtc
     4261 tctgaactgg acgctttggc agcagaattc cgagaccgtt ttggacaacc tgttcagcaa
     4321 gttaaggacc tcttcttcca attgcgcgta aaactactgg cagcgaaagc cgggttgacc
     4381 tcgattaacg ttgaagcaga tcaactcgtg ctgcgttttc cgcaactgcc agaaggcgcg
     4441 gtaaaacgtg aacttgcgcc gcttggttat caaaccagga ttggtaaaaa tgcttactgg
     4501 atgaagttca gcccggaaga gaacgagtgg cgggaaaact tgctgaaggt tctgggtgct
     4561 ataatcgctt catgacaacc atattaataa ttgaagacga agttaccctg gcgcgcattt
     4621 tgcaatcata tcttgagcgc gaaggctacc gtgtgctttc tgctcaacgc ggggatacag
     4681 gtttggacct ctgcctgagc gagaatgtgg acctggtcct gttggactta aatctacctg
     4741 ggatggatgg tgtagatgta gccaccgagg tgttgaggga aaaagatatc ccgatcatca
     4801 tggtcactgc tcgggtagaa gaaatagacc gtttaaaggg cttggagctc ggggctgatg
     4861 attacattac caagccgttc tcaccccggg aggtcgtcgc gcgggttaaa acagttctgc
     4921 gtagagtgaa tcgcagcagg gtggaggagg accgtttctt aattccaccg tttgagattg
     4981 atatagatgc gcacctggtc aaaaaagcag gtcaagaaat cgacttgacg ccatctgaat
     5041 tcgcaatact ggtgacactc gcgcgtcatc ctggtcgcgt gttttcgcgt cttcagttat
     5101 tggaagctac gcaaggacag agctttgaag gttacgaaag aacggtggac gcccatataa
     5161 aaaatttacg agcaaaaatt gaagaggatg caagaaaacc cgcttttata aagacagtgt
     5221 ttggagttgg ctataagttc taccgagagg atgagtaaat aatgaagttt tcgctccagc
     5281 aaaaaatgat tctggcgttt tcagttgtgg tcatcgtttc aattggcggg gtggtgattt
     5341 ttgccaacct ggatagccag cgccaggtga accggtatct gacaagggga ggacagtatg
     5401 gtgtgatgga gcttgttgaa gccctggaag actactatga atacaagggg acatggagta
     5461 tggtggggga agttgccgat gcttattcgc aacggcacgg gaggaaaaac gctcacggta
     5521 aagcctcaga actgacattg actgaccaaa accactttat cgtatggagt aacgctgggc
     5581 aggtgaaagg tgaaaaactc actggcgaaa tgacgtcaag gtcactcgaa attcatagcg
     5641 gcggacagac tgtgggatac ctggtcgttg aaaatggagt cttacagaca cctgatgaca
     5701 tagcgccatt tgtcaacagg ctgcgccgag cgatatttct cgcgggggga tttgccatct
     5761 tattagctat tgggctggca gtgctcgttt caaaatatct attagaaccc ataaaagtga
     5821 tcacttcagc atctactcaa ctctctacag gcgatttttc tacccgggtg gatatcaaag
     5881 gcaacgatga aattgccatg cttgccaaaa ccttcaacca gttggccaga aacctggagc
     5941 tggctgaaga gcgaaagcag tcgcttactg cggatgcggc gcatgagtta cgaaccccca
     6001 tcgctgtaca aaaagcgcag ctcgaaggaa tgcttgatgg cgtgctgccg ctaacacaaa
     6061 aaaatgttct gatcgcatta cagcagacca attttctctc acgcctggtg gaagacctca
     6121 gattgttagc gatggctgat gcgggcgaag ttcgtctcga aatgagaatt ctgaatattg
     6181 tgaaccttgt gcaacaggtg gttgctggtt ttcaggcaca ggcagcgctc gaaggaacca
     6241 atctggtgac cgtatttcgt ccagatggtg aagaattgtg gattcacacg gatgtcgacc
     6301 gggtttcgca aattttacag aacctgatct ccaatgcgct gcgctatgga caaaaaggag
     6361 ggcgcatcct cataagcacc aggaaagaaa gcgccaaact ggttatctcc gtccaggatg
     6421 atggaagcgg tataccatcc accgcgcttc cccatctttt tgaaaggttc tatcgtcacg
     6481 aacgcgcgcg aaccagagaa aatggaggtt cagggctggg actggcgatc agcaaaaaac
     6541 tggcgctttt attggggggt gacttgagtg gcgcgaatag ccccgaagga ggggcggtgt
     6601 tttcactgga gctgcccatc cgaaacactt agacacgcat agaagaacaa atgtgctata
     6661 atcaatgtaa ataaaaccag gaaaagagaa tgcagtttca ccttcaggca ccataccagg
     6721 ctaccggtga ccaaccggaa gcgattaagc agcttgtcaa aggcatccga cagggaatgc
     6781 gtaaccaggt gttgctcgga gctacgggca ccggtaaaac tttcactatt gccaaagtca
     6841 tccaagagct gcagatgccg gcgctggtga tggcgcataa caagactctg gctgctcagc
     6901 tttacgccga gtttcgcgag ttcttcccgc aaaacgcagt ggaatacttt gtctcttact
     6961 atgattatta ccagccggaa gcatatgttc cgcgccacga tctttatatt gaaaaagaga
     7021 cggatatcaa tgatgaaatt gaacgtcttc gcctggcggc tactactgcc ttaatgtcgc
     7081 ggaaagacgt gatcattgtg gcctctgtat cctgcatcta cggtctcggc aatcctgaag
     7141 aatacagcaa tgtggtggtt caccttgcgg tcggtgaaat atttagacga aatgcgctgc
     7201 tgcgacggct aattgagagc cagtaccagc gcaatgacct tgaattgcgg ccgggggctt
     7261 tcagggttcg cggagatacg atggagattt tccccgctta cgaggaaaaa caggctgtta
     7321 gaatcacttt tttcggtgac gaagtagaga aaatcgttga gtttaaccca atgaccggcg
     7381 agatcattgc ggaaccttct gctattgata tttaccccgc caagcattac atcgccagcg
     7441 aagcgcgcct gaaagtggcg ctggatgata ttgaaaaaga actgaacgac cagatccatt
     7501 atttcaaagc caacggcagg gtgctggaag cgcagcgcat tgagcagcgc tgccgttatg
     7561 acctggagat gctgagggag gttggctatt gttcggggat tgagaattat tcacgccatc
     7621 ttgaccagcg cccagcgggc tctcctccct ggaccttgat ggattatctg cccagtgagt
     7681 acctattagt actggacgag tcacacatga cggttccgca gatcaggggc atgtacaacg
     7741 gcgaccaatc gcggaaatca accctggtag aatacggctt cagattgcct tcagcattgg
     7801 ataaccgccc attaaccttc aaggaattcg agagggtgat ggggtatacc atttacacct
     7861 cagccacgcc ggctgatttt gagttgagcc gttctgacca gatcgttgag caaattatcc
     7921 gtcccacggg attggtcgac cctgaggtag aagtgagacc gacaaaaggg caggtggatg
     7981 acctgtatgg cgagatcaag aagcgcgttg aacagggcga gcgcgtttta gtaacaaccc
     8041 tgacaaagcg gatggcagag gatctttcag attacctgtt ggagttgggt ttaaaagtga
     8101 attatctcca ttcagaggtt gaaacgctgg aacgggttgg cattttaagg gacctgcgca
     8161 tgggtgtgtt tgatgtgatc gtgggaatca acctgctgcg tgaagggctt gacctgccag
     8221 aggtttcgct ggtggcaatc ctagacgccg ataaggaagg atttttgcgt tccgcaactg
     8281 cgttgattca gacgatcggc cgggcggcgc gtaatgtgaa cggcaaggtc atcatgtacg
     8341 ctgatacgat aaccggcgcg atgaaaaccg ccattgacga aacaaaccgc agacggaaaa
     8401 agcaaatgga ttacaacaaa gccaacggca ttgaaccctt cacggtggtg aaggctgttc
     8461 acgacctgac agagcgcctt tctgcttcat cagtgatcgc tgaagcgaaa ggtcgctact
     8521 cagccggcga agagaaacca cgcatgccga tcagtgatct gaaacgcatg atccttgaac
     8581 tggaaaagca aatgaaacag gcagccagcg agctgcagtt tgaacaggct gcggtcatca
     8641 gagatcgcct gattgaccta aagacatacc tggctgaaga atctgatctg ccaccctgga
     8701 aaaaagcccg cttgatggca ggtgaaatcg agtagtaagg attggattgg gcgaaacgtt
     8761 tacccactgg gggcaatcga gaaacgctta tagaaaacca cagggtttga ccattccact
     8821 gttacttggg ttacatgggc cattggcgga ccgacccgca cggtggccag tagtcgggtc
     8881 agtttgttct catccccctg ggcgagaatt tccaccttat catccataag gttgcgcacc
     8941 cagcctgtga gggcgagggc gtttgcctgt tgctgcacaa aaacacgaaa gcctactccc
     9001 tgaacggtgc cttcaaccag caaatgaatg catttaatct cagccatttc cattattggg
     9061 tcacgctgat gattgaaaaa aacaggatgg ctgcgtggtg aatcgctcgg gcagaggatc
     9121 aacctgcggg gagctactca tcttcttctt cctcatcttc agcgttgaaa tcatccagct
     9181 ctaaatcgcc aaggaaatct tcttcctcgt catcctcttc atcgccaggt gagaaatcca
     9241 gcagattcat atcgccaggc gtggagatgt cgagattctc catggctttc tccaaccatt
     9301 ctgcttcgtc ttcgttggaa gggttttcca gcaattcgtt caatttttcc ctgaccgcgt
     9361 ctccaccgat ttgtgaaaga gcccagatca acgccaggcg catttcttcg tcttcggttt
     9421 cctcttcaag caatgcgagc agtggctcgc gtgcggtgcg taattccagc tcgccggcgg
     9481 cgcgaattgc ttcaaactgg atttcgggct caggggaggt gagcatggct actacctgag
     9541 gaccccaggc atcatcggct gaacgtccca tagcacaaat cgccgacgtg acccaatgca
     9601 gatcgttcgt ttggtatgcg ttgcggatga ggtcaggcac ggctgggtga ctggaataac
     9661 ccagcgattc aagcgcgcgt tgtttgactc gcgttttgtc cttcccccgg acgatgttca
     9721 ccaggtgttg gttcagccct tctttcgcgc tgtctttcag agaatccagt tcacccagca
     9781 gtacgaactt gccgagcaat gaaatcgcgg tctcgcgggt cgtttcatct tcgtctttga
     9841 tagaaatatc gaccaagatg ggaaggatgg atttttcttc gcactcccat aacaggcgca
     9901 gggcgcaggt gcgcacatgg gggtcggggt cggcgagcaa agccctggcc aggttatcaa
     9961 aattcaccag ggtgtcctct tccaacacgg cttcaaggtc ctctaataaa gataccttgc
    10021 gcctggcagg gatggatggc cagcagttga gcaggacctt caggttggca ggcgggaggt
    10081 ctgagaaact gcgcagatag cgcgccggga agggaactga agcattttgt aatgctgaaa
    10141 gaaccgaaga aaatggtatg ggttcgcctg attctttatt catcggtcgc ctatttagga
    10201 atgatcaccg ggttaacgat atcgttgatg accagtaaga ccattaaagt cagcagcagg
    10261 gcataaaaaa tcatgtgtac tttcccctct agcgcggggg ggatcttttt cttgaagaac
    10321 agttctggca acaggaacag gattcgaccg ccatccagag cggggatggg cagtaaattg
    10381 gtgaacccca gggcgatgga aatgatggcg aaaaagtaaa gcgtgttcag cccggccatc
    10441 gcgggattct ccacagcctc ctgcttgtcc agggtttgaa cctgcgcgta gatatcgtat
    10501 atccctttta cgctcaccat gcgcgcttca gaaggggcaa tctgcccgct gataagcctg
    10561 accgggacca tcaacaactg gtaaccctgg tcaagggtgg cgataaacgc tcttggcagc
    10621 gcctgaatga agctgacttt ttgggaaggg ttcattatcg ttataccgag aggaccctgt
    10681 ttttcaggcc attcagcgcg cggggttacc ttgatcgtca gcgcaacacc ttcacgcagc
    10741 agcacgatct caacctcctg accgaggtgg gacgaaataa ccttggagag agcgtccatg
    10801 ctggtgattt ggacattgtt tactgattgg atgaggtcac ctgggagaat accggcggcg
    10861 gctgcaggag tttcatcagt gacagcggcg accataaccg aagagggatc aggcgagccg
    10921 atacgggtga agagaaaaga gatgatgagc atccccacga gcaggttggt caacggcccg
    10981 cccaacaagg tcaataaacg cctggaaggt ctggctgcca aaaaactgcc tggttcggac
    11041 acttctccgt tctcgccttt catgcggcag aaaccgccaa agggaatcca gtttatggtg
    11101 aaatccgtac caccggcgcg gaacaggcgg aacatcttgg gcgggtaacc cagaccaaac
    11161 tcctcgactt cgatcttgct caggcgcgca gcgaaaaagt gaccaagttc atgtaacagc
    11221 accagcgcgc cgaaggcgag gataaattca agaatggtga ttgcggtgct accgattgcg
    11281 ttcatagaag tccaatcaat gttttcaata ttaaaccaat tataaacaag aacagatatg
    11341 aaaaacataa gaaaaaagag cgggattgct cctcgctctt tctatttgta gaaaatgagg
    11401 tgtcattctc gatgtttgtt acggcgagta tagatagcga tgccgataat tccaagaatg
    11461 aacccgagta tttggaagaa ataggcaacg aagtttaaga tcaaataaat ggttgaattc
    11521 catctcaata gcgggtccag tacgtggatg accatgagaa acggaattgc tacacccata
    11581 accatcaaaa tgatggctga ccagatcaat ttcctgggat ttaggcggtc aatgatcatc
    11641 taagcagcca acaggcaaca ctatgctcag gttctggttc aaattctggc ggagcctcta
    11701 cttcacaaag ccctctaatg atctttgggc accttgggtg gaaacggcat cctttgggcg
    11761 gattgatcag gttaggaggt tcacctggtg gaacttcttt cagcttatgt gcgttggcac
    11821 catcagggtc tgaggtgccg gtgagcaacg cctgagtata cgggtgtccg gcgttgttca
    11881 gcaagtcttg aaccaatgct ttctctacga tttgaccggc gtacatcaca aaaatccgac
    11941 gtgaaaagta actgaccgta gaaaggtcgt gggtgatata gataaccgcg agattcctgg
    12001 attcttgcag gctgcggagc aaccggagga tttcaaccct gacggaggcg tccagcatgg
    12061 aaacaggttc gtctgccacc aggaagttgg ggtttaagat catggaacgg gcaataacga
    12121 tgcgttgttg ctggccaccg cttaacatgt gcggaaattt attcaagtaa tcttcaactg
    12181 gaaaaacttt aacttcttcc aacgctttgt gaaccatgtc ttgaatctgc tgatggtcct
    12241 taatcccgtt gatactcaat ggttctgtca gaatacgttg aaccgacatg aacggcgcca
    12301 tcgcaccata ggggtcttgc tggatgtaac cgatgtgcga acgatactgt ttgatttctt
    12361 tcgacttcat ttcgcctata gactcacccc tgaaaaagat ctctccttcc gtggggcggt
    12421 gaaggcccag aattcctttc atcaaggtgg actttccaca tcctgattca cccacgacag
    12481 cgattgattc tccattgtgg aggtcaaaac tgacgccatc aaccgcgtga acataccctg
    12541 cgtgaccaaa accaaatttt cttagttcga accaaatctt taaatctctg atggagagaa
    12601 ggacacctct ctcgcggatg tcttgttgga ggaacggttc tgccttctcg tttttgtcaa
    12661 ttatgtgatc catgacatct cctaccttta ttggtaaagc caacatttaa caatatgatt
    12721 ttcaattgtt actgcaggcg gttcttcttc gcacttttca aagcgtttgt ggcagcgatc
    12781 cttaaagcga cacccggttg gcgggttgat caaggatggc ggttggccgg tgatgaagtc
    12841 cggttctttc acctggcgta agcgtgggac gctatccatc agcgcgattg agtatggatg
    12901 gaaaggttta tcaaagaaac gggtcgcatc acagacttct acgatctgtc cagcgtacat
    12961 caccgcaacc cgatcagcca attcacttga ggttgcgata tcatgggtga tgaggataaa
    13021 gctgatctca aggtctttct tgagcgtttt caacacattg aaaatattcg cctgggttaa
    13081 aacatccaat gcggatgtgg gttcatccag gatgatcaaa tcaggcattg tcaccaacgc
    13141 catggcaatc gctgaacgct gcctcatgcc accactgagt tcgaaagcgt agcgatcgag
    13201 gaagctattc ggcacaccga cgtgcgcgaa catcttcttg gcttgcgcaa ttgcttctgt
    13261 ttttgaagca cctgtatgga tgataaaggg ttctgtaacc tggtcgccaa ccttcaacac
    13321 cgggttcagc gagttcatag ccgcctgtgg aaccatcgaa atggaacgcc acctgacttt
    13381 tcggcgaaac tcttcatcgg agtactgcat ggtatctgtg ccattcaata aaaccttgcc
    13441 cgaatacttg tcgatgtttc tgggtaatag acggagtatg gccttcgcca gcgagctttt
    13501 accgcaacca gattcgccca atatcacaat ggcttcattg tttttgaggt caaggtcaat
    13561 atggtcaacc gcctgcactg gaccgcgcat agtcttaaaa tagagaacaa ggtcttcgat
    13621 tttgagcagt gtaggtgagt tcgccattat tgacctcgca aacgcggatt aaaaacgcga
    13681 tcaagagcat agccgacaac agcaaatgcc agcccggtga caactaagag aaacgacggt
    13741 tcgagaaccc aataataata cccattgaac agcgcgccac tgttataaga ttcatcgatg
    13801 actttacccc aggtgggaat ggttggatct cccagcccaa gaatcgccag agaagattct
    13861 aagaaaacat aggacggaat caatgacaca aacccaggta tgagcatggg aattaggcgc
    13921 ggcaccatat atttgaagat gatgcgtgag ttactcgcgc cgtaggatcg tgacgcttcg
    13981 atatagggtg actccttgat ttgcatgaac atcgctcgat aggtcagtat tccagcgcca
    14041 aagatactta ataggatgac gcaggttaga atgacaaaaa tactgcgcga atagaacgtg
    14101 ccgatcatga ttaagatggg caagaatgga agcaccatat tgacattggt aatgcgttgg
    14161 atgagcacat cgatccaacc tccgaaccag gtactgatag ccgagataat cagttgtgtc
    14221 aaagaggtgc caactgcggc caccaggccg aacatcaacg cgattggtgt tccccataag
    14281 agggcaatgc tcaagtctcg ccttttatgg tcagttccgg caattccaga aactttgcca
    14341 tagaaaacgg cttcagcgct gactgtcgaa ccttcatcaa aggtaacgcc ttctattatg
    14401 agtttatagg tgcctttgag tggcttacgc ggtttgctgt caggctgcat taacaaggct
    14461 tcctggattg ggatatcttt atactttttt actaacttct gatcctggct caggtaatag
    14521 gccagagagc gttctacctt gaaattcccc agtttaattt ctcgttcatc aggcgtgatt
    14581 atagataaac caacaaaagg tgggctgctt gtataggtgg acttaaaaaa cacgtttatc
    14641 tcagaaggga aggcgtctgc tgtgaataca aagggcatct cgtaaatgac cgttgctttt
    14701 tcttcggtct tgaccacact gggatcatcc gtttttaaaa tgatggtggt cggaagctta
    14761 tttttgcgaa aactattaat ccaggccggc atggcgtact tcggactggc gtaccagtcc
    14821 tttgattcac cgcgccaaag gctgatcgct cgatcgtatg gtagcgcaat cagcgtgtaa
    14881 atggatagcg cgattaacaa gaggatgatg atcaaccccg caacagcgga gggatatttg
    14941 aaaagttcat taaaagtacg tttaattgag ttcatgctct gggtccctca tttccaacct
    15001 gaacacgggg atctaccaga gcgtaaacga tctctaatag aaaaaccgtg atacccagca
    15061 ggtaagaaaa aatgactgct tcagcaacga tgaccggggt atcaaacaat ccgattgcct
    15121 ggtaaagcgc ccgtcccaaa cccggccatt ggaaaaccgt ttcgaaaatt ggtgctccta
    15181 cccacatacc aataaccaga agggcaaagt tggtgatgat ggttgggaga gtagggcgaa
    15241 ggatgtattt ttgggtaacg tctctttcgg ttaatccttt tgcctttgcc atttcgacat
    15301 aatcttcgct ggaataaatc aagaaaaatg tgcgccagga aaatacgcta agaaaaatcg
    15361 aactgatggt gatggctgcc acaggcagca gcatatgccg taataaactc aaaatatatg
    15421 ccatggtttc tttaggcgga ggagcatcaa ccattcctcc aaacggcaag acacgcagaa
    15481 cagctgcaaa aattaaaatg agaaagatgc cgtaaaacca gccgggagca gaagaaagcg
    15541 gcgtgagagc gatcacaatt ttatccataa cactcccgta gtgccgcgat aaactcaaag
    15601 caatcatcaa tgccaggaag aataaaacca gatcggatgt accgaacaac aataaagtag
    15661 gtggcagtcg ttcgatgatg atattccgta cagatgaaga accgctattg ctggtcatcg
    15721 acatggccct gccaaggtcc agcgtgagcg cctgcttcag gtaaaggaaa cttctcacaa
    15781 ggaaaggttt atcaaggcca taaatacttt cctggtcgcg aaccatttcg gctattcgaa
    15841 tattccgaac ctcaggagac agctttcgta atacaggatc gttggctaca ataagaccga
    15901 cctgttcacg gatttgcgcc tttcttatcg tgtcaacata tccacccatg ttcgcaatca
    15961 gtacggtcag gtagacaccg atggtaacag tgatcaccat catgaccatg cgactggcgg
    16021 tgtattttaa aatccgcaag gctgtgctgg atcttttttc tttaattact tcttgttcta
    16081 aactattaaa gctgtctatg gttgggctcg catctaccat aatgaactta ctccttaaat
    16141 aggcaaaact cggtaggggg ttttataccc ctaccgagta atgtattagt tttgtttatt
    16201 ggacaacaaa ttcatattca gagaaggttg ggatgctcac aaccttggat gagactgctg
    16261 ttagaagttt ggctccgcct acttctagct tcccagtcgt ctcagctgag agcgacactg
    16321 cgtaaagtcc ttcttctgtc atcgtggcgt tacctttttc gaccagttta cctgtcgcgt
    16381 caaagagcat gtatgagaca aagttcaatt tcgcgctcgg gtaagcctcg tcatcatagg
    16441 ttaccgcaac tgtaaagtca gcagcttcgc ccttggtaat tgccagaggt ccgtcaattt
    16501 cagcatccgc gatcatcggc gcgccaaacc cgctgaattc atctgatggg aagatgtaat
    16561 cagcgtagcg gcttaatgtc acggtacctt cggtcgggta aacctcgtcg acgtagaaga
    16621 cgccggtccc gatcatcagg tgacctttat cctggaacca tcccaacagg ttggcgtacc
    16681 tggcagcagc ttcatctgca gtgatgtatt cacttagagt tggggcgaag gggattgtac
    16741 cagcagcagc ggcttcatcc aggtatttct tttggattgc cagagtttct ccagcgatga
    16801 ggctggtctg gtcaactcca agctgagttg ctttggcttt gctgaaggcg atcttctcat
    16861 cactctcggc gagcaccgca ggggtgagat catgccaggc aactggtcca tagctgaacg
    16921 aaggatacca ggcagaaacg ctgttctccg catccagggc aaaggcattg tcgtaggtct
    16981 cgatggtcaa cgggtcgact gatgtaatct tgacgcccct gaagtgagac aggtaggcat
    17041 ctactgggcc tttcatcgtg gagtcgaaga tggggctgcc ttctttgccg ggcgcgaagt
    17101 tcatgatcat gccgtagaca acatcaccga tggtgaaggg gctgccgtca tgccaggtgg
    17161 tatcccacaa ttcgggccgg tagataacca cggatttggt tacggccgtg ctgtaatcac
    17221 gtccggccag gtcaacaacc gatacagaag gatccgtgga ttctgctacg taacccaacc
    17281 accagtcaag ttcaccctgg cggtcagcca cagcatccgc agccaggttg ccatcgatcc
    17341 attcaacttc accgggaagt tcagcatcaa cgaatgcgga tgcgtcccag ggaaggccat
    17401 atttctccgc ggcaaaatcg gtgaattctt ttgttactgc tgccatggtt tcaaccgtca
    17461 tgttcgtggt gtcaaacgcg gcaactaatt ctgcgcgttt agcctctacc tcagccttga
    17521 ctgcttcgat ttctgcggct tttgccttgg cttcttcaac tttaaccttc atctcagcta
    17581 cagtaagcca cttttcattg acaggatccc agtcagccca ggcgtcctca ggtacttcca
    17641 ccgcgtcctg gaaggaaagc ttgaaccagt cttcaccatt ttcgataatg ggtgtgccgg
    17701 tcttggcgac cagttcggct ttttcaacca gtttaggcat attcagcccg gtgaacgggt
    17761 tggggaccaa accccagtcc atgatgccgc gataaaccat cgagtcatct gtccagttgg
    17821 aaccattgat ggggttaaac ggctgaacca gaataccaga ctgggcaact cgaaccacgc
    17881 cgcccacttt atctgcccaa cgcatagtgt atggccaaag cggggctgag gcgaaaccac
    17941 cagcgaggtc atacgcgccc tctaagggtt tccgataagg cgagatggca gtattgtcat
    18001 ttacccagac gccccaccag gattctttca tcgccaggtt aagaccgtct cgaatgagct
    18061 ggtctctttc ttccatgctg gtgaagtcgt tgtagagcag tttctggttg atcacatcaa
    18121 attctggtga aggatcgtat ttgctaaaga taggcagggt ggtttgtacc agacccgtgt
    18181 tgaattgcgg aatctgatag ccgtcatccc ggctgatagc ggtgctgatc cagccgccgg
    18241 tgtaaacgtg ccattcgcaa agttcggggg tagcgctcca cacgatcgga ctggcttccg
    18301 ttctggtttt ctctagccgc tcaactttaa atcctaaggc ttccagctgg gaggcaaagt
    18361 actgaccgat gccaaggcgc tggtcttcgg ttctaatgac gactttaatc gttacgggtg
    18421 cgccgttaaa ttcccaaaca ccgctggcgt tcttggtggc gccgagggcg gtcatgcggg
    18481 cgtcaaccgc tgcctgtgct ttttccatat cgtaggcata ggtgctggta atggcggcca
    18541 tcacatccgc gtaacgagcg taatcaggaa aagcgccagt gaaagaggtg aacttcggaa
    18601 ttgccaatcc tccaaagaac tcctgaacca cgtaatcgcg gtcaaaggcc cagttcattg
    18661 cctcacggaa ttccatgtca gtgaagggat tgagtgaacc gtcgtcgcat ttcaccacgt
    18721 tcaccatcaa ctggttgctg gaaccataga cggtagcata gcccaggttt tcatccgctt
    18781 ttactttagc gaatacctca ggatcttcaa cgctcaccgg gtagatatca atcgcgccag
    18841 cctgcagttg agctacgact gagtcggcat cggcaacggc ggtaaagatc actttgtcca
    18901 gccaggcgcc tttgccattg tgaggaatgg ccgtttcaac cacgggggct tctgtggcgg
    18961 ccggaacctc aggggctgcg gttggggctg cggttggctt ggcgcaggcg ctcaatacca
    19021 tggtcgcagc aatcaaaact cctagaattg catataactt gcgcatgtag tttctcctcc
    19081 taatttgaat tttgggcttg cttggtacta acaaagcgag atccttaagt ggcacctcct
    19141 ttttaaatga ttactttacc tgtctgcttc ggacagctta actttatgac atttgatgaa
    19201 aaaagtcaag gaaagtgacc tgaaggacga aacaaggggc atattaaata agtaccgtcc
    19261 cacctccagc tccactggtg agtacttcgc ttacgccggg gatcgcccct aacctttctt
    19321 ttacaatgct cgtggcgctt gattcacaga taacatgcac gttgggtccg gcgtcgatag
    19381 tgaaggcgca gggtatgcct gctcgccgcc agtctttcac tgcttgcata agcaccagcg
    19441 tctgcggcag ccaataagtg atgggcgggt tggaggtgaa agcgaccgcg tgaagaaggt
    19501 tgctgtcaag ctcgatgagc tgtgaaagca ggtcaaagtc ctttttttga atggcgttgc
    19561 ggcaaaggtc aatgcgtcga cctgcgtccg cgaccctcgc gctttggagc gggcttgtgc
    19621 gggcgaggag atgaccttcg cttgaacctg tctttttatg cgcgtcttca acgatggcga
    19681 tacaatcagt caggggccaa tgctcaggcg gggcgattga aaccgcgtac gaatcgatat
    19741 cggaggaacc cggcagccat tccacgtaac ctgggggaat cgaacgcgca gcggaaccgg
    19801 aaccgcgcct ggccaggcgc gagaggtctt tctcgctcat ctccatgccc gcggtgcgca
    19861 ccgcggcaac agtgagagcg gcgaaggcgg cggctgaaga agccaagcct gcgccaaccg
    19921 ggaagttgga ttcgctcaag atgtgggcgg ggactgaaaa accgcggata cctcgaatca
    19981 ggttcaaatg ctggctgacc ctttcaaggg agggaccgct ctgccttacg ccattcaaat
    20041 caaaaatatc ccgcggcagt gaactgtcaa aggtgaccat tgtgcgggtg gtcagttccg
    20101 cgaggttcat cgaaatcgag ccgttttgag gaagcctgag ggcatcatcg gtgtttcccc
    20161 agtatttaat aaaggcgatg ttggggtgag agaccgcggt tgctgtggag aaaatcatcg
    20221 gcatgatcct ttgaaatcgg cgtttctaat aggcaaatgg caacaattga attttacctg
    20281 aaactgattg ataatacctg aagagcaatg atatcccttt tttctttggg cggtctaact
    20341 tgagcggttg gtcttttcat gcggaaaggt taccataata caagaatgcg ccaggctcga
    20401 cattctcctt ctttttttat gcaggcgaag gtcatcaagg gagctagccg gagagggaaa
    20461 tcgagtactc acgggcaacc gcgctcaaac gttttgaaca cgaggaggat agatgatgga
    20521 gaaggtgatt cgcttcaacc tgacgattga acctggtcag caggggtcct attttaccgt
    20581 tcccttcgag gtcccccagg ggattgaatc gctcgaattg acctatgaat atcctcggcg
    20641 cttgaaaaga aaagaagaac agccaggaga gatatttaca gccactcctg aaacgaatat
    20701 cgtggatctt ggattgatcg cgccagacgg acggcaggtg ggggcttctg gctctgaaaa
    20761 aaccaggatc ttggtgagcg agcagtacgc cactcccggg tacggcgcgc gtaccgtttc
    20821 agcggggcga tggcaggtta ttgttggtgc atatcaggtt gcgccagaag gagttgaggt
    20881 gctttatgag atcacgctca aagaaaaatc gctgcgcctg ctcaaaggag atacgcatgt
    20941 gcactcgatc gcttcagacg gcgtacacac gtttgaagag ctggcgctga aagccaaggc
    21001 taatgggtta gattttttgg cggtcactga ccacaatgtg tttctgcccg ctgcgtccat
    21061 tccgtatgtt cccgggataa cgatgattcc tggcgtggag tggacccatt accagggtca
    21121 cgccaatttt ctgggcatcg acgcgcccta tgatgaaccg ttctttacaa attcagtgga
    21181 agaagccagg tcgcgcttca cctgtgccca tgagcgcggc gcgctgatca ccatcaacca
    21241 tcccttcgaa ggcaacagcc gtttcttgtt tgatttccgc cagctgcctt ttgattgcct
    21301 ggaggtgtgg aacgggccga tgcgggaatc caacctgaaa gcagttggct tgtggcaaca
    21361 gatgctcagc agtggtatgc gcgtggccat cactggcggc agcgattatc accgcgacac
    21421 accgtttatt ttcttgggcg ggccgacgat gggtgtatat gccctgtctg ctgggatgag
    21481 cgatattctg agcgcgctgc ggaaggggca tagttacatc accttcgcgc ctgatggacc
    21541 atccatcgag atgcgcgcgg gagaagggat tatgggcgac caggttgact ggcaggagga
    21601 aaaggagctg cgcgtacgcc tgcaagggct acaggcggga gatgtggtgc tcgtggtcac
    21661 cgcgcaggaa aacgcggcgg tgtttaccgc cacatcagag ggcgagctgg attttgccta
    21721 cgagatgagg gaaccgggtt ttgcccgcgt ggaggtgctg cgtgccttcc ttccaggctt
    21781 gccgttattg ccagcgctga tgagcaaccc gatttatttt aaggcttgag caaagacaat
    21841 cgagtggcga tatcctgcaa caggtaacct ggctccagcc acaatccata gatgacctcg
    21901 ctggtctggt tgggggtgag cgaacgcgcc gggttgatcc aaaggaagtt cgccaacagg
    21961 gtaagggcca acagcagcca gagttgccct ggatggttgg gcaatgcctt gccaatcaga
    22021 attaatgcaa aaggtaacag ggggagaaga tattccattt cgatgggaat gcgcaggaac
    22081 atgatctcca cgcttaagat aaccgctaaa gaaagccagc tcaaactgtc tgggcggggc
    22141 aaagttggtt tttgcttgtg aaacaaggcg gacaggcata gaaaacatgc ccaaaggacg
    22201 accggaaggc tgaaaaacat caggttcttg taaagaaagc gtcccacacg cagcacaggc
    22261 gtccacagag ctggatcacc ggtgctcaac accagccagc gacgaaagtc ccattccaga
    22321 aagtccaatg gggggaggaa aaaaacgatc gacattaacg cggcgcaaaa agaggtgagc
    22381 aggatccagc ggcgttgtgg cacactggtt agaaaaagaa aacacaggaa cagtcccacc
    22441 ggtaagacgg tggataaacg gcagcctaca gacagcgcca gtgaaattgc agcaggtaaa
    22501 ttacgttggt gaagcaacag attgaaaccg aggaagcaga agcccaaagc ccaaagataa
    22561 tcgatggtgc tggcagcgct cacccagaag aaaggctgca ccatgagcac agcgctgagc
    22621 aaagtggggt ggggaacggc gtaatgacgg catacttgcc ggaaagaatg aagcatcaca
    22681 agagacatgc aaacagaacc caggttcgtc agcacgctgc cgcctaattg atttaagaaa
    22741 aacacgaaaa actcgtgcac aaaaaagcct ggcagacgcg agggcaggta atcaaaagtg
    22801 gcgatgaaat attggccggt gcgcaccact ccaagagaat cgctatcgct gccgtaacca
    22861 aagaaaacca atggtatgta aagcactgaa gccactacca gcaataccca ggaaggcaac
    22921 gcctctactt tttcagtcca gttacgaagt gtaaacccgg atttcattgt attcgtcaca
    22981 tcctgttcgg ctttggcagt gaggggaatt aaggttaata ggggtcaaaa acgcgcggca
    23041 cacgctgatt gatctggcag gtaacttcgt aagtaatggt gttgagcttt tcagcccact
    23101 cgtaagcgct gatctcttca gctccgctgc gaccgatgag aacaacttca tcgcctactt
    23161 ttacatgggt ttcagcgccc aggttaatca ttgactgatc catgcaaacc cggccaacga
    23221 cgggataaga aacaccgtca atgagcactc gacctgtgtt cgagaataaa cggttgaacc
    23281 catcagcgta gccggccggg atggtggcaa tccaggtatc ttgtggcgca aaccaggtgc
    23341 gtccgtaacc gatggaagtg cctttttcca ccttcttgat gaatgaaacg cgggtgcgca
    23401 ggctgagacc gggtttgagg tcaacgcgat gagaagatgg ttcaccgggg tagaacccgt
    23461 aaatcatgat gccggggcgc accatgtcca tccagctttg tggataatcg agcacaccgc
    23521 ctgagttggc gcagtgcacc agaggaactt ttgcgccgat ggcgtcttct atggtcagga
    23581 cgactgattt gaaccggtcg atctgggctt gcgtgtagtc attacccatc gaatcgctca
    23641 ccggcagatg ggtaaagacg ccttcaagca agaggttgga gcagcgtgag tggatgaatt
    23701 ttgccagttc gggcgcctca cctggtgtga cgccgatgcg gcccatgccg ctatcgattt
    23761 tgaggtgcac cctggcagtc agttttagtc gctgacaaac gcgctgcagc gcctgaatat
    23821 tcgctttatc gcaaaccgtc agcgtaagct cattttcaac agcacattcc atttcttcag
    23881 ggaacgccgg gctaaacttg agaatcggga ggtggatacc ggcctcgcgt aattccatgc
    23941 cttccggaac ggttgccaca cccagccagt tcacgccgat gttttgagcc atgcgcgata
    24001 cctccaccgc gccgtgaccg taggcgttgg ctttgacggc aatcagaatc ttgcattgct
    24061 gcccgatcgc atggcgaatt ccttctatgt tgtggcggat attcgccagg tggatttgcg
    24121 cgtgagtttg gtaaagcatg cacttctcta tcaggaggat atcccgttaa tcatggtttt
    24181 tcgaacataa ggcgaggtac tggcagcaag cagggcgcca taccccgggt aaaaggctac
    24241 ctcatcaccc acagcgagcg gttgttgtgc gtcctgcaca tctaatagaa gatggtcgct
    24301 gcttccgcca agaacgatga tgccgggatc ctctggagtc aggttgtcaa tcactacatc
    24361 ctgccggcca atgttgcaga tggcgcgcag acgcaccccg cggtcgacga attcaggggt
    24421 tcctccaaaa gcatcctgtc ctcgttcacc gatggggacg gaaggttttc tttccagttc
    24481 gatgatctcg gcgaccaaac gagcggtatc ctgccgtgtt ccaggaaaag gagaacggtc
    24541 gagcacgttg cgcccaagca ggatggtttc gccaatacgc agcatattga tctgaagtgg
    24601 cattttatca gccaataaca aaggcaggtt agcgctgttg ccggcgcata acaccggcaa
    24661 ccgtataccc aggctggttt cagactggtc gcgcagatca ataagctgct gcatcttttc
    24721 cgtggtggga atgacaccgc catagcaggc caggttgcag cccaaaccgt tgacctcgat
    24781 gcctggaagg cgaatgatag cccttaaaac ttcatccagc cttgaaggcc aaatcccttc
    24841 gcgcaggtcg cccaggtcga ccatcaggat gactttatgc gtactgtgag ctgccattgc
    24901 cgccgcggat atttttttta tggtttccag gtcggagaca agcgagatgt ctgctgcgtg
    24961 gataacctct tccacctggc tgggggttgg gatgcgcagc aacatgagtg gaagagtggt
    25021 accgttttgc cgtaacgacc taaggttttc caggcgagaa tcggccagca ggtcagcgcc
    25081 gctctcttcg agcgcgcgca taatcactgg atgagcgcaa acgactttgc ccacacaggc
    25141 aacctggata ccatggtcat ggcataaatg gatgatcgcg cgggcgtttt cagcgatgcg
    25201 ttctgggaaa agttccaaac gaggtgtgaa caacttactc aaatctcctt cctgacccaa
    25261 tcaacaaaag tggatttcac ttcatcgggg tatttttgcg cgatggtacc cagcgctgaa
    25321 aaacggtagg cgtcatccag caggatgggt gtgccgggct ggggaagcag gtatttgccg
    25381 gcgccggtgc agacgcgttg tagaatctca actccccctg tgaccctggt aagcgctcct
    25441 ccggtgccaa tcaccctttt caccgcggtc aggtcttttc ctttgaccac ctgtttgcga
    25501 ccagatggcg tataaaggtc agttacctgg gcagcgtgac gcctggcgct gatttctacg
    25561 gctttggtgc ataaccagtg cgtcagttcc agctgtttat cggttacggg gatggcctgc
    25621 aaatcagcca gcctatcttc actttcccaa tcctggacaa tttcaatgat gtgtttcgcg
    25681 ttgacataaa cacccaggtc tccttcaacc gtcctttttg cgcggggctc tggttccagc
    25741 aatttcaacg accattcctg actgccgtca gtgacgctat gcacatcggt ggttgcccca
    25801 ccgacatcga ataccacgca atcaccaacc tcttctgcga aaagctcgct ggcaagcagc
    25861 accgcgccgg gagtaggcag gatgccatgg cgggtcaatt caccaaggcg ggccatcccg
    25921 ggagcgtgga taatatgtcg gttaaacact tcatgaatga tacgcctcaa aggttcgata
    25981 ttcaagacgt ccacttctgg aaacacatta tctgcgatga gcaagtcgat atcttgcgtt
    26041 ttgaatattt cagtaattgg ctggcgcaaa acgcggttac cggcgtaaac caccggcacc
    26101 tgaagcccaa ggttggcgat acgacgggcg ttttcaagca cagtctcttt ttccccataa
    26161 tccacccctc ccgcgagaag gataatgttg gggtggattt ggcggatctc tgacagttcg
    26221 aattcatcca gcaggccggc ggttactttt tttacgattg cacctgcccc cagggcggct
    26281 tcacgcgcag cgcgagcggt catgttgtag gttaagccgt gcacggtcat acgcaatcca
    26341 ccagcggctg agctattgat aaaaatttca acctcagggt cgagtgggtt tgcgccgtct
    26401 ttcacctgct gcatggccag ctcaactccg ggcattacgt ctccctgggt gatactggtg
    26461 ggcgcgaacc cctgcgccgc ctggacgaga gccccatgtt gagaacgata aaaaccgttc
    26521 accttggtga tcgtcgaacc cacttcgatg gtgagaatac agataggttc aggcaatggg
    26581 caccttaatt gaggggatta agccagccca cgtttctgca ggttcttgac catggcattg
    26641 accacgtgga tacccttggt gcctctgccg aaggtggcat ccaggccggt ttcagccgcc
    26701 atttcggcgt tgacctgtgt gccgccagcg atgagaatga gacggtctcg aacccctcgc
    26761 tccacacaca aatccgccag cttcttcatt tgagagcggt gaacatcatt gtggctgatg
    26821 atggttgaaa tcaagatcgc ctgagcgcct gtctcgatcg cagcgtcgac cagtttactc
    26881 accggcacag aagtaccgag gtaatggtat ttgacgccat atttttcgat accgccgtgt
    26941 ttgatatcaa ggatttctcg taaacctacc gagtgttcgt cttctccgac cgtacctgcc
    27001 acaacggtca acccgacttt tttcactacc tggcgcaagg tttcttcttc gagcatgatc
    27061 tctttggcag ggattttaag gtcagtgaga ttaagctcaa aattcacttt ccctttgacc
    27121 tcaacgaagc aaccttcagc cggatgcatg acctgaatgt tgatgacctc agggtcgatc
    27181 agccccagtt ttttcgccat ttcaacgcca tataccctgg cgtagtcttc aggcaagggg
    27241 acaaagaact gagttaggat ggtgccgtct ccagcccatt cggcttcggg tttgatcttt
    27301 ccccctttac ggtagggata ggtctcctgt aaacgctttt cacaattgtc ctcggggtcg
    27361 agctcatcga tgtagacgat tttttcaggt ttgcacaacg tgcagccctg aatggcatca
    27421 caggggcgtt gcaactcagc cgggacgttg ttgtaaccaa aatggtcgca tactggggca
    27481 aagtaatccg cgtcacgctc cacgatggta tctgcggcca cgccgccttt tccatctctg
    27541 gcgataccgt caccgttgcg ttccgggtac ttggcagaat caacgaagaa ccctttttca
    27601 accgcagcaa aatagccgcc tgtctcaatg acttcctcca gaaaagccac tgcgcgcact
    27661 ttcaggtcac gcaccatttc cttcatcgga ccattggttt ttaacgcgac cagatccttg
    27721 atgccgtcta acgcggccca ggtctgtttg accgtttgga cgccgcggat gttattgtaa
    27781 tgccagggca cattgcgacc ctcatccggt gtgatggtac tctggatatc cgcgctggta
    27841 agcatggaga tcagtgtgtc aatggtatgc gtacggatgg cttcttcaat atccgcctcg
    27901 atatagcggg tgttctgctg tgcgcgcatc ttgaaatctg aaaagaaatc acgcaacgcc
    27961 acggcgtaag gcaggtcata ccataacttg ggagctggcg gcgcgttggg cgggacagtg
    28021 gaaagaccga taaggtcctt atccatgcct accgctactg agaaggaaca attgatgccg
    28081 tgctgcacca ttaattcggg cattaccaac caaccggctt tggcagtggc gttggcattg
    28141 tgcgcgccat caatttggaa gatacgggcg ccgttcatca cctttttggc ttctgcagcg
    28201 tccacaaaag aacggtacat attgacgttg cgatagagca cgttgtattg aggatcttga
    28261 tgggcgccat taatgccttc ttcggcaaaa agcaccgcca cttcaggacc agccacgccc
    28321 gagacgtagc tgtggaagtt gatagggcgt ccgacttctt cttcgatttg gtcgcaagcc
    28381 ttgcggctgg cgcgaatctg tttacgggtg atgggaaccc cgcccactcc ttcaggcgtg
    28441 ccttccatca atccatcaat gtgactttga ccagtggtgc ggatgaccat caggtggtct
    28501 gccccgtgcc aggcagccat gcgcatacgg cggatatcat cttcgaaccg cccggaggcg
    28561 atctcagtgg tcaccacaca acttggctgc gggtcgatat ggtcgaagta cttggaagca
    28621 ggcaagccaa tgctgttctt cagcggttta gacatatcgt ggtaatggaa aggacccatc
    28681 tctacaccct cctggggtat ttcacgccag gtccagcctt tacggcgagg gtggtaatgc
    28741 tccagtcctt caaggatatg ttcaatatcc attttttgat tggggtcgag tttttcgggt
    28801 gtgttggtca tttttgtccc tttcaaaaca atcccgagag gatttctttc ttttcattga
    28861 ttaaagccgc ggcggtgggg atgctcacac cctgcttttc ggcaaccttc agcacgacat
    28921 gaccagcgcc ttttcccaac agaccggctt caagcacacg gtccacgacg ccatgggcag
    28981 tgatgctatc gattcccatg cgtaacaaca ctgagcgctc aatcgaaggg gatgtatgcg
    29041 ttcgggcaag ttccacaatt ggttccacca acctgtcgca taattgccag aattggtttt
    29101 ttaactcctc atcgctgagt ttatttaatt ctttctgacg ctctgtaaat cgtttgatac
    29161 ggtcttcaga cattggtctt ctccaggttt tcgttatttt gcggaaggga taggaagttc
    29221 tccctgatcc aatcgggggt tttctttata tccagtgcca aaaaagagat atcactttct
    29281 gaccagagag acggggcaag ttgtttgccc gcgttttgca ggtattcaag gcgcaggcgg
    29341 tcaatatcct ggcttttgcc ctgtacctgc tcaagttttt cggggatgat gatggtttta
    29401 ccaggaatgt catccagcgg gtcaccgcgg cggatctcaa tcccgttttc gcgggcaaag
    29461 gctaactggc tgttctggtg tttccctgcg cctgtgtatt cggtctcctg aaccaccaca
    29521 acctgttcgc gctccatttg gcgagccagc gcgaccgcgg cggtgaggct gatattcccg
    29581 gcgggaccgc gttcaagacc ttcgatcttg gtgagcagtt cggtgatgta aaacacctca
    29641 ccctgcgtta ccagctggta ctcgtccatg tagcgcaggc tcctggcggc gttacggggt
    29701 acatctacgc ggtcaggcca ggtggcaaat ggcaccccga aaccagtgtg acctgtcgtg
    29761 aaagattttc gattgaaatc atggtcggat gccatgtgca gcccttgaag attgactgag
    29821 caggcaacaa tgcgtgttgc gcagctgcca gcgtgcagta aaccgcgagc ggttccggta
    29881 aggttaccgc cgccagcgtg cgtgatgatg accgcgtcag ggtcacggtg gtacctagcg
    29941 cgggtttgtt ctgcgatttc cacccccagg ctttcaaccc cgcggatacc aaatggggta
    30001 tacaggctgg cgttgaaaaa accggtttct tcaagggtgc gtaaaagcat ataaaaaagc
    30061 tcagggccga ccgtcaactt gagcacttcc gcgccgtagg cttcacaggc gcggcctttt
    30121 tcaacaattt ccggctgacc gataccgcgg ctatcgtaca cttcctgaac gatgatgcat
    30181 ttcagcttgc gctgagcagc ctgcgaggca accgcggcgc cgtaattgcc actggtagcg
    30241 gcgaccatgc ccgcataacc tttatgtgct gcttcatacg cgctgattga cgcgcggcgg
    30301 gctttgaaac ttcctgatgc atttgccgct tcgtccttga ccaggatggt ggcgcctttg
    30361 cctggaggag aaatctggcg taccgcttcc gtcaggttcc gcaattcgta aagcggggta
    30421 ttccccactt tggtttcttg ttggatacga aaaatctcat ccatcgtgta gccagtatcg
    30481 cgcatcatgg cttcgtaatc aaaggcaacc gggctggaaa tgtatttctc gtaatccatg
    30541 cccatggaag acttcatgat gtcatttttt cgtgccatca cggcagcgta ggacacgtct
    30601 ctttggctca tttttcatac tccgttccga tggtaagcag ttcgggtacg actgtgccaa
    30661 agctatgatg gtagctgggg ttcaccactt ttagctcccc gctcaattca cgaccgataa
    30721 tggttttcac gcgcactttt tgcccgattt tcgcgtcttc aagcagaaac cctgataccc
    30781 agaggagata gggcaccttc tttgtatcct ccgggagcga tgccgctcgt tcttcaggtt
    30841 ttaataccac ctgctcaatc tcgacccagg ttcctttagg aatgatgttc attgtgattg
    30901 ctccaagtag ttcagcgctc ggctcaaacc tggcgccgat acgctgtttc tcccatcaac
    30961 gcagcggggg ctggcaggtc gatcatgcgt ttgaggcctg ctgtggcagc gaccacatgt
    31021 gggatcatat tgaccgcaag acccatggtg gcgatacctc cagcaatttc gggcttgatc
    31081 gccatattga tctccggttt accaaaaata tggatgtaat ctccggtatc ctgtccttca
    31141 agttgagggt gaatctgttg agggtggata agacggataa cctctttatc tccccggtaa
    31201 ccaatgccgg tatgggcgca cccggctacc attcccggtt gaaccttaac atgaggggtt
    31261 tctcggtaca cgttgctgat gatgggctcc cgcgcctgtt ctatacggtc aacgcccaga
    31321 cccagcgcat cactgatcat atggatggac tcaggaaagc ctacatgccc gacgatggaa
    31381 ccatcttcaa tgcctgcttt aaaggcagcg ggagtgatgc ccacaccctg ggatttcatc
    31441 accgttggac cgtatgggct taagtcattc acccgcgaag cttcgatgcg ctcgacagtg
    31501 tgactcccac ctgatagcat cacgaccagc aggtcaagga cgaacccggg attgacgccg
    31561 gtccccaata aggtgacctg gttggcttta cacaacacgt ctaattgcgc cgccaattcg
    31621 gggttctgcg cgtaagggtc agacatttct tcagcgatgg agatacaatt aatcccggct
    31681 tgaatgatct ttttaaggtc atgcatctgt tcagacagcc aggatgttgt ggcgatgacc
    31741 acgatatcaa cccgctcttt gtttaggacg gaatcaggat tggaagtgac ggttacaccc
    31801 aggctgcgat cgagcttcaa cacttctcca aggtccttcc ctgcataatc ggtgcgggaa
    31861 tcaactgcag cgacgatttg cagccctgat ttttccagta tcaggcgcgt catgccgcta
    31921 cccattgcac ccagacccca ttgaagaaca cgaataggct tcattttgac tcctgtgagt
    31981 ttttttaatt ttttttgaca agcaaatttt agcatagaag acgcgaaggg ggtgcgcaag
    32041 ggatggtagg caccttgaga gcaggtggaa tgtgctcaaa atgcgaacac attgaaatct
    32101 acgggcaagc ctccgttggt gaaatggtaa gatgcttgct gtggaggtaa accaaggttg
    32161 gctgtaagct ttttatcgta acaaaggatg cctgcctgcc attgtgaaaa agccgtagta
    32221 aggagattgc caaaacgggc atacaaattg cgtaagtctt tgctgccgct gatgcgtacg
    32281 ccataaggcg gattggtgat cagccagcca gggtttgagc gagggggaga taattctgaa
    32341 atggattgac gctggaaatg aatgacctcg tttaaaccag cgcgcgaagc atttgaaatc
    32401 gcagattgaa ccgccccctg gtcgcggtca tagccaaaaa tatgtgaatc ctgccggata
    32461 atcccatccc tggcagagtg aaggactctc tcccactcat gcggaacaaa acctggccag
    32521 tgcatgaagg caaaatcgcg ggcgaccccg ggcgggatat tcctggccag cagggcagct
    32581 tcgataggga tggtgcctga accgcagaaa gggtcgatca aggtcatttc tccgctccaa
    32641 cctgacagca acagcaaagc cgcagccagc gtttcgcgta agggcgcttt ggtgctctcc
    32701 agtcggtaac cgcgtttgtg caacagagca cctgaactgt caatgctgat ggtgcacaga
    32761 tcattcacca acctgacaag cacgagctgc ccattcttgc tgggttgagt ctctttttta
    32821 gagttggaaa agcggtcatt gatggcgccc acgacccttt cagctaccgc atcagaatga
    32881 taaaggcgtg atttgtggca ggtaaccgaa agatgaacag gctgcccagg gctcagataa
    32941 cgctcccact ccaggcgtga ggctttcttc cgcaattcag aaaatgcagc ggcgtagaat
    33001 tccccgagcc tgacgcttac acggctggcg gtgcgtaaat acaggttgga gcggtataca
    33061 tcctccaaag agcctgaaag gagccatccc cctggatcat ctggtaaaac acgatttttt
    33121 gatgctgccg tcgcaggctc aggttccaag atggtcaggt tgagcgcctt gagttcttgg
    33181 gcgaggatca tctccagtcc gggcgcacaa gacacaaaga aacggagcga ctcaggcgac
    33241 ctaatcatgg cttccgatta cgggagaggg ggtgtttttc aaatggcttt tgtgtgccgc
    33301 ccgtctttga tcggccgggg ttggtgatga tttgcttctg cagcgctttg cggtgcaggt
    33361 ttttttcaac aaagagcttt ttaccactga aggggtcttt ttctgtgaaa tacatcacgc
    33421 tggcgtaagt cgatggggta ggggtaaaaa tttgaacctg ttccggttgg actcttaatt
    33481 ccttggtgac gaattcacgg agctttttca tttcccgctc gctgcttcca ggataggctg
    33541 caataagata gtagctgaga aactgttttt taccggcctg gcgggaaaat tcgaggaatt
    33601 tttcacggaa cagccgcagc gattcatgag acggtttgcc catcaggttc aacacaactg
    33661 gatcagtgtg ctcgggggca acctttagtt gccctgaagt gtgatagcag gtgatatctt
    33721 ttagatattg cattccagac cggcggtcag ccagtaccaa gtcataacga atgccggaac
    33781 caacgctcac tttttttact cctggcaggt tccgtaattt acgtaaaagg gcaacctgcc
    33841 tggaatgatc aactggcaac gcagggcaga tattgggaaa aacacagcgt ttatcctcgc
    33901 aggcaccgga gaccagcttt ttgcggcatt caaacccgta catattcgca gtcggcccgc
    33961 tcaagtcagt gatcattcct ttgaaatcag ggtgatgaga gagcttctca gcttccttca
    34021 gaatcgaagc ttcgctgcgt gataccaccg tcctcccttc gtgaacggct atggcgcaaa
    34081 aattacattc gccgtagcag ccccgatgtg tgggaatgga gaactggatg gtttgtaagg
    34141 cttttacctc gccctgcaat tcgtaataag gatgatgtaa ccgttcaaag cccaggtcgt
    34201 aaatggtgtc gagcgcttgg gttgatgggg ttggaggagg gggattttgc actaggtagc
    34261 ggtcgccttt cttttgcacc agacctcgcg cggtgagggg gtcattgttg cgataaaaaa
    34321 ggtcgaacat atgaatgaac gcgtcagggt cagcggatac ttcctcaaaa gatggcagtt
    34381 ccagataggt tgagggggct tcagcagcca ggtagcataa accgcgtatt tcacgcggcg
    34441 aacgcttttg tcgtaaggcg agggccagct ccacaatgct gccatctgcc atcccgtaca
    34501 gcagataatc cgcttttgag tcgaacagga tcggcgcgcg caggcggtca gaccagtaat
    34561 cgtaatgtgt gatgcgcctc agactggctt cgatccctcc caggacaatg ggtacggtat
    34621 ttttgtgata acgcttgatg aggttggtgt aagcgatgac tgcacgatcg ggccggcgaa
    34681 tattggctcc accaggggta aaatcatccg tacggcgttt gcgcaacgtg gctgtgtaat
    34741 ttgcgaccat ggaatccact gtacccgcgc tgacccccca aaaaaggagc ggttcaccca
    34801 gcctgcggat gtcttccgag ccggacatat ccggttgggc aatgatcgcc accctataac
    34861 cttgtgaaat taaaattttc ccgattaccg ccaggccgct gtacggggtg tcgatatagg
    34921 aatcgccgga aaccaggatc acatccggtt gttcccagcc caaatggacg agttcttcac
    34981 gcgtggttgg aatgaacacg atagttccct gttgtcgaac aaactgagta taacatctca
    35041 aacgcgaagt aaaaaaaagc agccgatagg ctgccttgat cattcatctt caagttgttc
    35101 gtgttcgtcc aggtggacat gatcgtggtt cagctcttcg ctggttggca ggcggagggc
    35161 gataatcttc acctcgaatt ccaaatcgcg gccggcaagc ggatggttga aatccaatgt
    35221 tacagaatcg gcagtaattt cttcaataaa cgctgaggat tcaattcctt catcatcctc
    35281 gacggtgact tccatatcta cttcgagtgg aaaatcctct gggaattcac tgcgggggat
    35341 ttcaataata tcctcttccg aatagacgcc gtagccatcc tctggcttta cactcacatg
    35401 tttcgattca ccaatgttca ttccttcgat ggcagattct agcccggtaa taatatgccc
    35461 gtgcccctgg ataaactgca gcgggtcgtc ttctgtggct tgatcgatga gctcaccttc
    35521 gattttcaac tcataagcca tactgacaac caggtctttg gatactttgc gtagatcgtt
    35581 tgatgggttc aagttagctc actttctttt tcgtagaatg aatgagccat gattatatca
    35641 cccgggggta taaaaaaaac accgattgga atgtgaaaac aggtgaggag gtttactgcc
    35701 cgataaaaac ttcgcagctg cctattttct cgaaactggc caatttgttc actgcctgca
    35761 gccacaattc ggtagacacg ttcgtagaaa agtgctctgg cagcaggtcg gcccaatgaa
    35821 tttgattgat tacctggtca attccatttt ggctgatttg agcgcaaaat tgagagatta
    35881 ccttctgacc atgcgagaga attgtctggc gctctaatgg ggtgagaggt ggttgagaag
    35941 gggaagaggg gttttccagc cccagccagg tttcaattga agaagccgaa aaattgtcaa
    36001 ccagaaaata gccgtttgtt tttatgtgga acttgcgctc gatcttcctg atcacccggt
    36061 caggtatttt ggcgtcacgg gtcaaacgga aaaaggagaa tgagtcgtct tcttgctctg
    36121 tatctgcggg tgggtagagc gagacaaacg ccaggtgagg gggggggcat cacgcacgaa
    36181 caacccccat acagatacca gcataggtga atccccaccc agttctgaaa cttgaaagat
    36241 taaataattt tgctgggctg atataggctc aaggctgttg ttgggggctg tctttttcag
    36301 gtgaccatcc aagtgagcag ctgcctggaa tccaaaatag acagacacca aaaagagaag
    36361 ggcgatcaat gccagggcga ttttcttcat gtgaagacag gctttcaaat aagtgacaga
    36421 tgataagatt ctatcatctg tttgacagat acatccctaa tcaaccaagg cgatggatta
    36481 acggtcacat gaccagcagg gtaacagagt aaatcagcag cccgctggcg aaacccacgc
    36541 aaaaaacgag aagcacgatg gcaatttttc gcatcatccc tttatcctgt gaagccagat
    36601 aggttgatcg cacaatggtg cgcagcaggc ttgatttggt caacaacgtg atgatgaggt
    36661 tccagaaact cagttcaatc gaattgataa tctcagcaat catcgcaccc tctatcttga
    36721 agtggtgagc ttcaggatct tgccattttc atctgaaaca acccgaaccg tacgtgaaag
    36781 tgttttacca tcggctgttt ttgcctgccc atggaagatc aactgatatt tatcagcagg
    36841 taaggctttc acccgtggag gagccccttt taattcgggg aactgcctgt aaacggaagt
    36901 gcaaacggcg ttttttatcc tggcgtccat ggcgctcctt tcacctggct ttgcgccgca
    36961 taaaggccgg aacgtcgagc tccgattggg cggctgccag ctcggggtga acatccggaa
    37021 tattctcctg gtaagaatta ccgggagaga gagaggtttc tttgtctgtg tcttcagcag
    37081 gtgtcatcac aggtacaggg cgcttcatcc gttgtgggtc gagcgcagtg gtgccaatgc
    37141 cggttaatac caggatgacc tgaacccggt cttccatcct gtcatcacag atgatgccag
    37201 ggacaatctc ggccattcca ccgctctttt cctgcaaggt gttgagcgct tccgtgacct
    37261 caaagaaggg aaggtcgtta ccaccagtga agttggcaat gatcccgctg gcgtcttcca
    37321 aatcaataga ttcaagcatg gggtggtgca aggcgtgttc gagcgcgtca cttacgcggt
    37381 tttcaccttt ccctgttcca attgccagca gcgagccacc accattcagc atcatgttct
    37441 tgatatgcga aaagtccaca ttgatcaaac ctggctgggt gattagttcg gaaattccct
    37501 ggatgccctg ccgcaggata tcatctgcga gacggaacgc catttcaata ggcaggttac
    37561 gtggagccac ctgcaacagg cggtcattgg ggactgtgat gagcgtatct gtgtgctgct
    37621 gcagcattaa taacccattg cgagcgttcg tttgcctgcg cccaatttca aaggaaaaag
    37681 gcaggctgac aaccgcaacc actaccgccc ccacggactt ggccacccgg gcagcgacgg
    37741 gaatggcgcc ggtacccgtt ccgccaccca taccagctgt taggaagacc atatcagcgc
    37801 ccgtaagcgc gctagtgagt tcagcgatgc tctcttctgc ggcagcctga cccaccgccg
    37861 ggtcgccgcc tgcaccgaga ccgcgggtga tttttcgacc caactggatc ttgaggtcag
    37921 cctggctgtg ttgcagcacc tgagcatcag aattagcggc gataaattgc actccttcaa
    37981 gcccaagttc gatcatacgg tcgaccgcgt tttgaccgcc acctcccagt ccgattactt
    38041 ttagaactgg cggattggct ttgatgggag tgcgcgcaac gtgggagtgg aaggttgagt
    38101 aatccatgtt tacctctctg cgagttgagt tgcaatactt gtaccatccc cactgatacg
    38161 ctcaacgaca gaaccggaac ggcgtaattc ctcaagagag gtttcaggga aggactgttc
    38221 accgcctatt acggtaacgc gtctggcaag agctgcttct cggattgaaa agccaatcgt
    38281 agggcggtgt ttctgaacga aggcgcgggt aacatcgaga tgaaagtcag cgacgcccca
    38341 ttcatacaca gggataagca ggtaatgatc gatcgggtgg cctgtgaagg atttaattgg
    38401 cgtggattct gcttcaacaa ggaggtctgc tttttctaag ggtacgttcg catggttgga
    38461 agctccaggc acttgagcct gcatcaacag gtcagccacg ggcaaatagt cttcaggaga
    38521 tttataccag gcgcaggcaa actcctccga gtctggttct gcggagagta aataaaaact
    38581 acaacttatc acataatcag gaatggcggt tttcgaagaa ttctctttaa gcatttccat
    38641 gatgcttaag cagattccgg catgcacctc gggagagtag ggttgacctg gttctcgaat
    38701 tcctgcgccc aacatgaaaa tcggtaaatc ctggtcacaa actgttcgag ccgtggcctg
    38761 cagccattca aaattaaaga atccacgttg atcctgtcct tgcgcatcag tgatataggg
    38821 taatgatttt ggccatttct gcggcccgcc cgcgccgtaa tccaactcgt gattaaaggt
    38881 gaaagcgtaa gaagccaaac ccatttggtt gatcaaagat gaatgaccgc gtctttgtat
    38941 tgactgaaga gattgcttga ggaaggaaag gtcccagtaa cttcctccgg gttggagggg
    39001 tgggaaaatg ggcatcattt cggctttgac tgttgccgtg gctaaaggta aaaagcggtc
    39061 gacgaacctt tcaacaaggt tttgttgtac ccatccggca gcggaccagt tcgccatatc
    39121 attcgggcga tcaaaaagaa ttatgtgttt cacaccccaa cgcgcgtagg ccgccagaat
    39181 ggctttcagg tctgcgggtg aaggggtgtt gggcagcggc aagggaatgt gaaccaatgg
    39241 ggtaataccg gctttgatta atgcggtgat gaactgttcg gggatggcgc gggaggtatc
    39301 agagaggagc accaaccagg cgcacttgag ccgctgcagc acaggcagcc agttggaaag
    39361 gtctttattc gtataatggg aagtatccgg gaagtagtgg attccgaccc ggccagtgac
    39421 gagttccctg gttttcttgc ttttcaatat catgcggcac ctcgccaacc ctacgtttta
    39481 cgtaaccgga aagttgcaag aaatgagcca gagcaattgg cacaacccta attctgctat
    39541 aattccctcg ttatgatcct tgtcacgggc ggtactggat ttatcgggcg gtcacttatt
    39601 caggcgctgg tatcttcagg cgaacaagta cgagttttat tgcgcccttc gaaaacatct
    39661 cccaacttcc ctaaaggcat ctccgttgag gtagcggtga gcagcttttc agaccagcgc
    39721 agtttgcgcg ctgcgttgaa aggggtcgat tttttgtatc atttcgcagg ggctgaacgt
    39781 caaggactca acgcgaattt gaatcagatc gatgtggaag gcaccgccac attgatgcgc
    39841 gctgccaaag acaccaacct caaacgggtc ttttatttga gccatcacgg cgctgatcgg
    39901 gggtcggctt acccggtgat gaaggccaag ggcatcgctg agaattggat tatggatagc
    39961 ggtatccctt acacgatttt gagaacagga gcagtatttg gacccgggga ccaatttacc
    40021 atcccgctca ccaggctgct gcgcatttca ccctatttca ccttgatgcc gggagatggt
    40081 tccaaccttc tgcaacccat ctggattgaa gatttggtga cctgtatctt gttggctgcg
    40141 gcagacgata ataagcgaaa ccgggttatt cccgtaggtg gaatagagta tctgacttat
    40201 cttgaaattc tcaagctggt gatgaagcaa actggtatcc aaaggaaacc gctcggcgtg
    40261 acgcctgaat ttctgcgcgg gttaaccttg tggatcgccc aggtttaccc gaaattacca
    40321 gtatccatct tctggctgga ttatctggcg gttgaccgca ttaccagcct ggattcagtt
    40381 ccacgtgagt tcggtttgat tccagccagg tttaaaaaca agatcgatta tctgaataag
    40441 aagatttaac accgcatgcc tgaaaatcga gcgttctgca tccatgccca tttttaccaa
    40501 cccccgcgcg aggatccttt cacggggaag atccctgaag aagcgggtgc ggcgccattt
    40561 gccaattgga atgaacgcat tctggcgacc tgctaccagc caaatgtgga ggagcgtaac
    40621 ttcagtcagt tgagctttaa tcttggccca acactcgtcg aatggatgag ccaacacgcg
    40681 ccagatgtac tcgataggat tgtccaggaa gacgcgctta atgccaccct ttatggcact
    40741 gggaacgcga tcgctcaacc ataccatcac accatcctcc cactggcgag caatgaagat
    40801 aaacgcaccc aggtgttatg ggggattgcg gcttttaatc atgtttttgt cagggctccc
    40861 ctgggaatgt ggttacctga aacagccgta gattatgagt cgttgcgtgt gttggcatct
    40921 gctggtattc aatttaccat gctggctccc tggcaggttg aggtgattga aggagaatca
    40981 ccctatttag tcgaactggg tgcgggaaaa tcaatcatcg tgtttgtgta tcatggtggg
    41041 ctgagctcca ctgtcagttt tgatgccttt gcgacgagca atgcggatgc ttttgctgag
    41101 ttctacttga aaccggaaat cgaccgattc aaccccaatc aatttttatt gatagccact
    41161 gatggtgaat tgtatggtca ccatcaaccc tatcgcgaaa aatttctctc caggctgttg
    41221 gatggatcat ccaacggcat tggattgcgg gcgacatatc ccgctttgtg gatgaaaaat
    41281 caccaggtaa cgggaagggc gcgcattgta gaaaacacgt cttggagttg ttttcacggt
    41341 attgaccgct ggcggactga gtgcgattgc acggctggga gtacctggaa agcgggttta
    41401 cgtgaagggc tctccctgat tgcggcacgc atcgacgacg cctttagcga aatttgtgca
    41461 gcgcatgggt tggaccccat taccgcgaga aatgactata tccaagtaat gcttggaatg
    41521 gacactttcg aacagtggct gtcaaaaaca agcggcaaag aggtctcatc cattggtaaa
    41581 cgcaagctgg ccacgttgtt tagtgcccaa aggttgcgtc agcaaatgtt tagctcgtgc
    41641 ggctggtttt ttgacgaatt agcacgtatt gaaccgcgca acaacctggc ctacgccgcg
    41701 cacgcggtcg ttttgatgga gtcactgacc agcatggact ttcacagcgt ggcgcagccc
    41761 gcattacgcg aagcggtcag tcatacaagc ggtgaaaccg cctttgactt ttttattttt
    41821 gcctaccaac ggttcaagaa tttacagaag actgcccagg cgaatgttgg ttgaaatcga
    41881 atagtcggtc gaactgatag cctgattttg cttctaactt cttcaacttg cccaagagct
    41941 gattaagatt caagacaacc tcggtgatgg tggaagcatc cgcgcctggg tttgctatat
    42001 actggtaaag tgcgatgaga gacagccacc agacaagggt ttcaaatgat tcacggttgt
    42061 accacagcac atcctggtaa cggttcacgc gcaacagggt ttgcacctgc gggtctgtaa
    42121 accagcggac gattatttcc tccattggtt catttttcgt cgcgttgtac catccctggt
    42181 gggtgatgcc gattcgtaaa agaagggcgc tgttttggat ctcctggctg gtaacgccca
    42241 ttgattgcag tacctcaatg aaagttttat tcaggcggaa ttcgtcaagc caagcgaggg
    42301 actgttctga aaaatcttcc tggctggcaa ctttgcccag ccggcttgtg agtgacagca
    42361 tcaacaaagt tacccgcagg gcagggcttt gctccaaatg gttttcaagc gcgtcaagaa
    42421 acgtcgaggt tgaaggggag agtctagtga cgcgtttttt caactcttga gaaaacatga
    42481 gagcagtcaa gaagagagag ctggtttcct gttggagact gcgtaattca cctgagttac
    42541 cagtgtaaag cgccacgccc tgcaaaaatt tgcctatcct ttcctgtatt tcccgggctt
    42601 cagtactgtc caacgtcggg ctggtggaat atttttgcag taaatacccc aggtaacctt
    42661 gattgacaat cacctggaag ggttccaata ctggtttaag ggtcagttcg ttcaacgcct
    42721 gggctatatc cggcacgccc tgtctaccaa tcagtccgta taaagatgcg taatcatgag
    42781 cagaatcagc atgaacaacc tgaaaatcta gcagggtttg gtaatgataa gcgtgaagcc
    42841 ggaagaaaaa accacgatct aacacttcat ccaaggggcg aaggtattga agttgtgaga
    42901 tctggtcacg aaagaccagc cagccgttac ggtcagccgg caaatgcagc gcttgggcga
    42961 ttgagacctg ttccagtttt ttttcctgtc cataacagcg aagctgagga acggaagtat
    43021 gcacccaacc ttccgtgtct tcataccgat tattaaatac gatgagtgcg gcatttccag
    43081 cgtcatagtt cgtataggcg tacacattct cgttcacgtt gccattgttc gtgaaaaagt
    43141 catacaggtt gaagtgatca accccggcga acaggcggcg ttggtgtaat aagggaaaaa
    43201 tttctcgttc atgcctggag accaaatcgt tatctaccgg ttcatcccag taggctttac
    43261 ggtattccat accgtatttc tccgagaacc cttcaatttg gccgtgaccc agcataggca
    43321 gaccgggcat ggttgccatg agcatgcaga tgccgaaata cttatcactt tttccgaatt
    43381 gctcgacggc agtacgctca tcgggattgt tcataaaatt gacataacgt ttgagaattt
    43441 ccgggtcaaa ttccaaagtg ttgcgcatta gccggcggta gccggcattg tcttcattgc
    43501 gcagcatatt catgaaagcg ctgttataca cccggtgcat cccgagcgta cgcacaaaat
    43561 agccttccat aagccagaag gcttcggcga gcagcagggt atcgggggct tctactgcca
    43621 cccggtctac cacttcacgc caaaattcaa ccgggaacaa acggttgaac tcttcagcag
    43681 taaggctatg ctcggaacgt gatgggatcg cccctccgga gccaggcacc gggaaccaga
    43741 ggcgctggta atgttttttc gccaacgtca tcgccgcgtc aaaccggata attgggaacc
    43801 tgtgggcaac ttctaaaata gtttgaatga cagcctcgcg gacctcaggg ttgagatagt
    43861 ttaactgggc ggtgtcattc cagggcatcg aggtgccatc gtttccatgg tacacatagt
    43921 ccacctgtcc attacgcaaa tcctggcgtt tgaacaccac agcggcgtct gtacggtcat
    43981 agtaatgatc ttctatctgt atcctcacat caggatgcga ggaaagattt tccccattga
    44041 aactgtaaga aggatagggg ctgtaatcta aagaaaggaa gcggtcagga tgatggatta
    44101 cccagtcgga atcaatgccc atgtgatttg gaaccatgtc gctggccaga cggatgccaa
    44161 agcgggcagc attttctttt agcgctaaat acgcgttttc tccacccagg tcagccgcga
    44221 tctggtaaga atgcagcgaa tacgccgaag aaatggcttc tgggttgccg cataattttt
    44281 taatgcgggc agaagccggg ctgcgttccc ataaaccgat cagccataga cctgttattc
    44341 cccaacgaga taaagtctcg agctcatcaa ggggaatctg gtcaaggcgg tttatctcgc
    44401 gctgatattt tttgctcaac tggtcgagcc acacatagct attcttcgcc atcagcacca
    44461 gccgtggcat ccattcacgg tctttgctga acgcctcaat ctcagaagaa ccgcctgcct
    44521 gaaaaaatcc atcacggtca taaaccggga tggccgtgac gcctggcccg gtgatgccaa
    44581 gcttgttttc ttccttgata aggtcaaggc tggaaagcaa acggtacagg taatctccca
    44641 gtaacatagc ccaatgttcg cgaatgtact ctaattgccc tgtgatggaa tcagggactg
    44701 caatcgccgg gctgcgcaac atattcgtca atggaaagtt ggaaggccca aaatatggtt
    44761 gcagagcaaa aaagtgatta atctcagata taaacagggc gtaacgggaa tcgttgataa
    44821 aggttttttc ggcaaataat tctctatatg agtcaagggc ggggttttcg agagaaagcc
    44881 agagcatgat taattcttca attaacagtt cccgattaga aacgccgtca gtgttcccaa
    44941 gcaaatattc atgggcgtca acctgttttt gataaactgg cgagggagga aattcccgta
    45001 caaacaactc aaggaatgca ttcaaagcgg ttggggttga ttgctgttcg agataggcga
    45061 gcgcttgttt taatacctgt ggatttttct gttctttata tagtttgaag atgtggtgga
    45121 atatctcatc aatcaaaccc atggcgttca cctggctgg
//
LOCUS       DAOTLW010000012        40076 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1211_length_40076_cov_9.974089, whole genome shotgun sequence.
ACCESSION   DAOTLW010000012 DAOTLW010000000
VERSION     DAOTLW010000012.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 40076)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 40076)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..40076
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1211_length_40076_cov_9.974089"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1389
                     /locus_tag="P5516_03330"
     CDS             <1..>1389
                     /locus_tag="P5516_03330"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRT91502.1"
                     /translation="YYALVLFFIGAMVGLVLTSNLLLIFFFWEITAFCSYALIAFHND
                     DPKAVRGGVKALIITSTGGVGLLAGALVLFAYTGSTDINAFIGAAGKIPANILSLLAF
                     GFLVAAAAKSAQFPFQTWLPDAMEAPSPISALIHAATMVNAGVYLLARTYPVFQNVPY
                     WREAVMVVGTLSAILAAVMALTANDLKRVLAYSTVSQLGYMVYAIGAGGVFASQFHLF
                     SHSIFKALLFLGAGAVIHSIGTRDMTEMGGVGKKLPLVKWVFLIGSLALAGLPVLNGF
                     WSKELVLDAGLKGGPTWLYVLMAIVAGLTALYTARCVGLVFYGESRSEYHVHPVGLGM
                     KAALIPLATAALVSWIVVGPFSEMLGAHSLPFHEIKKMPLAELFHEILTLPTLVTLIV
                     IGLGILLWFTRSKLEGLTNGLRSIRWASEKSFGFEALNTAFVEVLNNTAETLRGTQTG
                     ILNWNVLAISAGL"
     assembly_gap    1390..1489
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1490..2283
                     /locus_tag="P5516_03335"
     CDS             <1490..2283
                     /locus_tag="P5516_03335"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRT91503.1"
                     /translation="PSGISAMLSGVVIEAGLLAMLRSMSFFGRVEFNWGATLLVFGCL
                     NILFGNLLALRQTEIKRLLAYSSLAHVGYMLVGFGMGFWYGQANGAGGGFFHLITHSL
                     MKGLAFLAAGALLYALHLQNDEHVPLTLSDLNGASRKYPLAAFALSVALLALGGLPPL
                     AGFMSKWQILAAVASAKNTLMILVVIFVGLNSVLSLGYYAPIVNRLYRRETSPAVENG
                     KPIPVMMVIPLVILVLALVVIGLWPASMDFFTEKATSSLLFPFFY"
     gene            2298..2609
                     /locus_tag="P5516_03340"
     CDS             2298..2609
                     /locus_tag="P5516_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91504.1"
                     /translation="MQILTSTPIALLIYLALVTLLYLVGKALAGKENPTPEKSSLYGS
                     GEEAPTSFASPGYRPFFLIAFFFAILHLGVLVIGSGEFSFKLIPILLGLILALVALIL
                     G"
     gene            2632..3078
                     /locus_tag="P5516_03345"
     CDS             2632..3078
                     /locus_tag="P5516_03345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HRT91505.1"
                     /translation="MNNPIQNVMEKIKTWARTNSPWAIHFNSGSCNGCDIEILATLTP
                     RYDVERFGIKLQGSPRHADVLICTGPVTLQARDRLVRIYEQMPEPKFVVAVGSCSISG
                     GVFDGCYNVVGDISEVIPVNVYVPGCPPRPEAIIYGIEQLLKTLQP"
     gene            3093..3602
                     /locus_tag="P5516_03350"
     CDS             3093..3602
                     /locus_tag="P5516_03350"
                     /inference="COORDINATES: protein motif:HMM:NF012549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRT91506.1"
                     /translation="MNTQEALEKAEAILKDLALSFTCPEENRLDAVIKADDLKKAVEA
                     LLVTNRWGYLSALTGLDNPEYEVDEDTREKVAVEGKGSLEVLYHFAAGAAITTLRVSL
                     PYDAAEIDSICDLVPAATLYEREAMELFGICFKGTPSTEHLVLPDCWPNGVYPLRKTF
                     KGLNEKAQG"
     gene            3615..4796
                     /locus_tag="P5516_03355"
     CDS             3615..4796
                     /locus_tag="P5516_03355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel-dependent hydrogenase large subunit"
                     /protein_id="HRT91507.1"
                     /translation="MPRQKSERFIVPIGPQHPALKEPGHFEFTVEGEVVTNATARLGF
                     VHRGMEKGAESRTWTQDLYLMERVCGICSHVHSMAFVLGVEKLANVQAPPRAQAIREL
                     VAELERIHSHILWLGVAAHEGGFDTLFMFSWRDRETVMDLLETISGNRVNYSANVLGG
                     VKVDINERQKDELLRGMDFLEERTRHYLKVVTEDDTFLGRTRNIGMMTFEEATRLGAI
                     GPTGRASGLLRDVRMDSPYAGYPDFPIQIVTAKAGDLEARFVVRIKELFESYRLVREI
                     VEKIPSGDLTTKIPRRIPEGETVSRVEAPRGELFYYIKSNGTDRPERVKVRTPSLCNW
                     ASVLTTAIGHKLADMPMILAGIDPCFSCNDRMVVLNGPAGQSLWNWEKLRQYGIEYYK
                     K"
     gene            4793..5746
                     /locus_tag="P5516_03360"
     CDS             4793..5746
                     /locus_tag="P5516_03360"
                     /inference="COORDINATES: protein motif:HMM:NF012373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit H"
                     /protein_id="HRT91508.1"
                     /translation="MNVTFPPLIPLLLFPGGLFLLASGLVFEYVDRKFIARYQNRIGP
                     RWFQPLADLVKLLAKEEVIPAGTIKGLFIALPIAALAAALTAGLSVPLFGFRPVFSYP
                     GDLIGTLYLLSLMTLCIGLAGANTENRFSIVGATRALTQLFSYEAPFLLALLGPAIAL
                     GTWNIGEINQAAGMNLWFILSQPIGFLAALIGLMGKLELPPFDAPEAETEIVAGALTE
                     YSGRGFALFRIGRDVELVIGLTLIASFYLGGFSSPLDFLWKTAVLLLITAALQSLFAR
                     LRIDQTVGLWWRVGAILLLVQMLVIIVGSSLAPFVSRLFGG"
     gene            5748..6176
                     /locus_tag="P5516_03365"
     CDS             5748..6176
                     /locus_tag="P5516_03365"
                     /inference="COORDINATES: protein motif:HMM:NF024634.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRT91509.1"
                     /translation="MPIGSMLGDIVKSFFKKPVTEKYPFVKTAAPENLRGKLVWNPEN
                     CSGCQLCIKDCPANAIELITIDKVNKRFVMRYNLDKCTYCAQCVVSCRFHCLEMSDDQ
                     WELAATHKEPFEVYYGREEDIKFLLEKAALGGPEEACPDA"
     gene            6145..6672
                     /locus_tag="P5516_03370"
     CDS             6145..6672
                     /locus_tag="P5516_03370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase 3 maturation endopeptidase HyCI"
                     /protein_id="HRT91510.1"
                     /translation="MVLKKHALTPDARVAVVGVGAELNGDDAVGLWVADRLARLVGKQ
                     ENLLVLNGGSLPESTSGALRRFHPMLVLFVDAADLGKTKGAIEVLETQRVGGTSFSTH
                     GMPLKMLMDYLGEELGCESVLIGVQPEWVDFDSPLSDRAKKAANRLVKELYHLLAVPN
                     LDEEELLAKKEDLGV"
     gene            6672..7382
                     /locus_tag="P5516_03375"
     CDS             6672..7382
                     /locus_tag="P5516_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91511.1"
                     /translation="MAEYNQRHSKATTILALIPAYNEAERITAVITGTRALLPVLVVD
                     DGSTDDTAAISSALGAEVLRQLPNQGKGAALLAGFRYALEMGYQAVVTLDADGQHDPQ
                     EIPLFIHAFEEGADLVIGMRDFKKMPFPRNISNTIGTWLFSWALGQYCPDNQSGYRLH
                     SRRMIETSLQSAETNFEFEVEIITRCVEQGYLLRWVPIKTIYAGEKSHIKPLRHIRHF
                     FRLVRGTRHRLQQLKKQP"
     gene            7615..8070
                     /locus_tag="P5516_03380"
     CDS             7615..8070
                     /locus_tag="P5516_03380"
                     /inference="COORDINATES: protein motif:HMM:NF024773.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HRT91512.1"
                     /translation="MQNTRESIEKFLQLKKISVVGVSRNKDKFGNMLYRELKNSGYQV
                     MAVNPNADMVEGDRCYAGLGALPVKPEGVVVVVTPAEALQVVEEAAGLGITHIWLQPG
                     AGSRETDQKCEELNLNCISGECLFMYLEPVKSVHAFHRFIRRLLGRMPK"
     gene            complement(8106..10805)
                     /locus_tag="P5516_03385"
     CDS             complement(8106..10805)
                     /locus_tag="P5516_03385"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HRT91513.1"
                     /translation="MATAGLAKTYDFRATEQRLYRLWEEKGYFEPSNDPRKADFDASI
                     KPFVISIPPPNVTGELHAGHAMFVSMEDLMIRYHRMKGIPTLWVPGTDHAGIATQMLV
                     EKALAKEGKKRDGMTREEFLKHTWEWKDLYHQRITSQIRRLGASCDWTRERFTLDEGL
                     SSAVREAFVRLYEKGLIYRGPRMINWSPALRTAVSDLEVEYSEEPGFLYYFKYMLADG
                     SGAYLPVATTRPETILGDTAVAVHPEDERFQRFIGKKVIVPLLNREIPVIADEYVTME
                     FGTGALKITPAHDPNDYTIGLNHGLEMINVLDTSARMNENAGPYQGMDRFECRKKIWE
                     DMRAAGLVIKEQPYTMNVPKSQRSGEIVEPMISTQWFIAIKPLAEKALEAVRDGRTRI
                     IPERFTKIYYNWMENIRDWCISRQLWWGHRIPVWYCDDCGEIIVSRQDPSACPKCGNQ
                     NIRQDEDVLDTWFSSGLWPFSTLGWPEETADLQYFYPTSVMETGADILFFWVARMMMF
                     GLEFTGQVPFHTVYLHGLILDKDGQKMSKTKGNVIDPLEIMDDYGTDALRFTLLVGST
                     PGHDTNLDIKKVEANRNFANKVWNAGRFVLSAVEKAPAEPGSEPDWTLADSWIWGRLK
                     ELLRETDRLFQSYQYGEAGRQIYEFFWSDFADWYLEIAKIQMSQGGDAAYYTATTLVR
                     VLDACLRLLHPFTPYVTEELWGHLKGAALDKAYKPFNSRRTWEDALIIAAWPEAGKSE
                     GWEEQAISSFTVIQEVVRAIRNIRADKKITPGRKLPALISAGAKMDILRGQCATIAAL
                     AGLDIEHSHISRKITEDTTGFVSVVVSGVEIFLDLAEKTDSVQDVERLRKELEVVSAQ
                     VDRLEKLLASDFATKAPPAVVEKERAKLREFIETRDKLKAQIA"
     gene            11145..11789
                     /locus_tag="P5516_03390"
     CDS             11145..11789
                     /locus_tag="P5516_03390"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HRT91514.1"
                     /translation="MPKKVVQHSKPSWLTWAQRIQALAQSGLYYTSDVYDAERFRELS
                     SIACEIMEANTLSQPGEVRMVFASQSGYATPKLDIRGVVFKEDKILLVRELQDQGKWT
                     LPGGWVDINEPPSQAVEREVREEAGKIVKAVKLLALYDSNQHGHPPLPFHSYKLFFLC
                     EMLGETIPAPYETAEPSFFAQQEIPELSTTRVTHEEIQRLFEHLRDPSLPADFD"
     gene            11847..13265
                     /locus_tag="P5516_03395"
     CDS             11847..13265
                     /locus_tag="P5516_03395"
                     /inference="COORDINATES: protein motif:HMM:NF024808.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRT91515.1"
                     /translation="MKKILVGVCKMVVVLVLSVTASCAPRSSPTPAITTTPTARITST
                     ANYSTSTPTIAAINPHVDIQKLDGLQIRFMHPWTGESLKLLFHMVDQFNQTNEWGIHV
                     IMQAPGSAARVSEKIHEELTNQIASNVLVAPVSLLLAIDERFQQVVDLSGYIASPLYG
                     MDPSLVNDFHQAFWDEGVVDGKLMGIPAQRSALLLAYNHTWGQELGFTSIPDTPAEFR
                     RQVCAANASFRKDADTANDGLGGWILNTDAVSMLNWLLVFGANPYSNGGFTFSTTSSQ
                     DALTYLYGLKTDACAWVNRSAADAERFSRRETLVYSLWLQELDRQVAATERSGSKDEW
                     MLMTYPDGMKGQVLTQGSSYAVLHKDAESDLAAWLFIRWLSQPAQQFRLLTASGTLPL
                     GSQVMTQMEEYGAEHPHWLQIVNSLTRFTTPPVNADWQVIAPVLEDAGWQLWMGGTKE
                     DEITGLLAQMDDLAVELAERYP"
     gene            13265..13708
                     /gene="rnhA"
                     /locus_tag="P5516_03400"
     CDS             13265..13708
                     /gene="rnhA"
                     /locus_tag="P5516_03400"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HRT91516.1"
                     /translation="MTDEIIIYTDGGCDPNPGLGGWAAVIIEGKNRREISGAAAFSTN
                     NRMELTAAIEALKVVKKNLPITLFTDSQYLKRGIDEWLPRWLAKNWRGANGPVANKDL
                     WQELLRVTRDRKVNWRWIYGHTGNPDNERVDFLCRLARSQFRRSY"
     gene            complement(13692..15164)
                     /locus_tag="P5516_03405"
     CDS             complement(13692..15164)
                     /locus_tag="P5516_03405"
                     /inference="COORDINATES: protein motif:HMM:NF003716.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium/proton antiporter"
                     /protein_id="HRT91517.1"
                     /translation="MPESPEIILIIGAILILISVMVSKVSERLGIPLLLLFLLLGMVA
                     GSEGLGGIYFDDPLVAQWVCTVALSVILFSGGIDTEWKRIKPLFKEGLLLATLGVFLT
                     AAVVALFSRFILQLNWHVSLLIGAIASSTDAAAVFALLRSKGIRLKGNLAPLLELESG
                     SNDPMAIFLTIGILQWIQNKIDQPIEMILLFIMQMGVGLIVGWLMSRLSLFLINRLKL
                     GYEGLYPVLIFALVFLTFGLASLLGGSGYLAAYVLGLLLGKEDFLHKRSLVRFFDGNA
                     WLMQIALFITLGLLVFPSEIKPVILPGLLVSFVLIFLARPIAVFLTLIPFRYTLREKT
                     FISWVGLRGAVPVVLGTFPLVAGISDASAIFNLVFFIVITSVLLQGTLLPVVACWLKV
                     DAPLPVNSQAPIEVVSGQGLKADLREILITPSSPAVNKAIYQLKLPEEYLIIMVLRDG
                     EYIQPTGSLELKKGDILFSLSEEPVYLQAQNIINGVSTNA"
     gene            complement(15331..15402)
                     /locus_tag="P5516_03410"
     tRNA            complement(15331..15402)
                     /locus_tag="P5516_03410"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(15367..15369),aa:Cys,seq:gca)
     assembly_gap    15746..15845
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <15846..16046
                     /locus_tag="P5516_03415"
     CDS             <15846..16046
                     /locus_tag="P5516_03415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HRT91518.1"
                     /translation="QKGGNAHAEVHAAERSIQLLGGSLRRLSLVTLPGVVDERYLVVV
                     DKKAATPPIYPRRAGMPLKKPL"
     gene            complement(16048..17262)
                     /locus_tag="P5516_03420"
     CDS             complement(16048..17262)
                     /locus_tag="P5516_03420"
                     /inference="COORDINATES: protein motif:HMM:TIGR00611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HRT91519.1"
                     /translation="MHLTHISLTNFRLFSRLDMNVPRRILLLLGANAQGKTSLLEAIY
                     YLATFTSFHAQNDRQLLNFIATRENLAVARLVAQYTRAERAHRLEVRMIQDSNGNGGA
                     RLRKEVLLDGVKTPPAQVIGQFNAVIFLPQMTGIIEDGPEERRRYLNLTISQSNPVYT
                     RALADYNQALTQRNALLKLLNERGGDRSQLDYWDEILAARAALLITTRAVVIQELEEI
                     AKRVHERLTGSKEVLRLNYQPSYDPLPAPQGQFALPMQVQVQRSGLSQEKVKQGFIAR
                     LAELRAEEIARGITTIGPHRDEMRVLSNGIDLTDFGSRGQIRTALLSLKLAEVDWLKE
                     KSGQWPVLLLDETLAELDTQRRAYLLDYLGNAEQVLLTTTDMNLFPHDFREKCERWRV
                     VEGSVAQLGAKD"
     gene            complement(17266..17772)
                     /gene="def"
                     /locus_tag="P5516_03425"
     CDS             complement(17266..17772)
                     /gene="def"
                     /locus_tag="P5516_03425"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HRT91520.1"
                     /translation="MALREIITIPHLILRKKARKVTDFGRDFQVLVTDMIDTMREAPG
                     VGLAAPQVAESQRVIVVEYGDEEDEEKPKKLFVVANPEIISSSEDKVIGIEGCLSVPE
                     LVGEVERYHAIIVKGQNRFGKPTKIKASGWLARIFQHEIDHLDGVLYPDRALRVWKPA
                     PEEDIPLD"
     gene            complement(17816..19540)
                     /locus_tag="P5516_03430"
     CDS             complement(17816..19540)
                     /locus_tag="P5516_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HRT91521.1"
                     /translation="MEILPDFGTPLFAEKPRFFLSFSYTYSMETVEPATVSLLAGLFP
                     LTLLEGNDLSQVAAQFEVLNYPRGSRLYAADDIPDYFYWIISGNVRLAGGAMRRGKAE
                     QVLGAGDYFGAEVLSRSDYRFSDAVCLSEVKLVRVNEQDLIQLCANLPTIENAFSLIY
                     QTHKFRARLFFPWLEKGEKVNLVCRRHPFFLALRVVLIGGAVIASFAGLLSLALSSKS
                     VALILLAGTLLLIGTLLTAWSALEWGNDFFILTSDRVLVQKKLTGFFDNRQEAPYTAI
                     LSTGFETSFWGRLLRFGTINLRTYNGKLSFKRLPVPEIIFDLLEYQRRSIAQKKHQSD
                     YAEMRETLQHRLEGATVRTKNAAAGKHTGGLKTTYKSGSFLDLAARFFGLRHESAGAI
                     IYRTHWWRLLGKTFLPTLLLLIVVLLVLLKLAGFLPSIAADHLYIGSIILAVTSWAWW
                     LYQYQDWYNDVYIITSEQLVDVNRKPLGKEERRSAPVQNIQTVEYKRKGIIGLILNFG
                     TVHIQIGNQELSFDDVYNPAAVQAEIFSRFKHRDQKTLRLEQEKLVDWIKAYDEMKKE
                     DKDPFDSQ"
     gene            19539..21056
                     /locus_tag="P5516_03435"
     CDS             19539..21056
                     /locus_tag="P5516_03435"
                     /inference="COORDINATES: protein motif:HMM:NF024748.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HRT91522.1"
                     /translation="MACTETDLMMKRVGHFSIFRWLSLAFILAALVLTVFQLVVFSRL
                     RSSFSTGTSIAGVDVTGLSMEQAASRVTQAYSIPVELRYNNSVIQVKPGTLGFSLDMT
                     AMMTAADQLRSAEPFWPSFIAYLFNQQPSASEIPLRAEIDDKQLRAYLVNEIASRYDE
                     EPAAYLPVPGSVTFEAGTPGKKLDVDRSALLVSNALRSPAARIVNLALITGKLNRPSM
                     DNLRILLQQIVDVNNFDGVVELYLQDLKNGSEMQMAYQNGESLTPGIAFSADSTIKIA
                     VMVTAYQEVAEPASTKVVQLLQSMIAMSDNTSTDNLMREVLSPTLGPLTVTSTMRKLG
                     LQSTFLDGMFYIGAPLLTGNIVTPANSRRDVFTDPDPYNQTTPTEMGLLLADIYQCAQ
                     NDGGSLVAVFPGEITPAECRSMISLLTQNRIGVLIEAGLPDGTQIGHKHGWAIDAMDG
                     VMHTVGDAGLVYTPGGNYVLVIFIYDQNQIVWDNANQLFADLSRAVYNYYNLTAE"
     gene            21175..24888
                     /locus_tag="P5516_03440"
     CDS             21175..24888
                     /locus_tag="P5516_03440"
                     /inference="COORDINATES: protein motif:HMM:TIGR03663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03663 family protein"
                     /protein_id="HRT91523.1"
                     /translation="MNSAENVESKPGWLDRPLYTWIPRITIEHIVIALIILMAVLSRF
                     YAVGLRVMSHDEVNHVVPSYELYQGRGYAHDPVTHGPLQFHLVAGSYFLFGDSDFSSR
                     IPAALFSIATIIFVIFGFRRYLGRVGALLAGAFFLVSPYMLFYGRYTRNEAFVALFGV
                     VMLYAVLRYLEDGRHRHLYLFTVALALHFTAKETAFIYTAQILIFLAVLFIRDVVKHE
                     WPQGGRRDFFIFSMMAGLLFVGTALGAAVMDASIKPGNAEAVAAPAVYHTVMLISLAL
                     AALGMILAIVVLLGSMGWRVVREIRSFDLLILTSTMILPQLIAFPINLLGWNPLDYSQ
                     TGMIRTLLFLLGAVVISVTLGLLWKPRLWLINVGVFYAIFTIFYTTFFTNGQGFFTGM
                     VGSLGYWLSQQGVNRGSQPWYFFAFLQVPMYEYLPLVGTLIAVVIGVKHRLFSSLPGV
                     APALQTAEQVEVPSQLPLPALEGSEETPAEKPGKLPVLSLLVFWGLTSLVAFTLAGEK
                     MPWLTVHVTLPLLLCAGFGFGYLVDSTPFKQLANKKGLLVLALLPVFITSLGGTIAAL
                     VGTQKPFAGKELEQLKATSTFLFALIATLASGGLLWKLLLRWSQRAILKLLTLTLGAI
                     LMVLTARSAYQASFINYDTGKEFLVYAHAARGPKDVLEQVEDISQRTTGGKDIRVAYI
                     GDALYPYWWYFRDYPNKVWLKDDLTRDLLNYPVVISDDEHYSKTQAILKDQYFETKYK
                     RLVWPMQDYFNMTWERFWKGFTNPQMRQAIINIWLNKDYRLYASLANNSGLTLENWQP
                     SANIYLFIQKDIVERIWTYGAIPQQQETAETDPYAGKYFELTPDRVFGSSGSAEGQLT
                     MPRGLAVAADGSVYVADANNHRIQKFSADGAFLLSWGSYATAEGGNAPGGTFNEPWGV
                     AVAPDGSVYVADTWNHRIQKFTRDGKFLTMWGVPGLAEEPDRFWGPRGIAVDREGTVY
                     VTDTGNNRVVIFDENGNYQQQFGRSGINPGEFDEPVGIAVDGNGRVYVADTWNQRIQV
                     FEPAGKEGYSVLRSWDVHGWFGQSINNKPFLALDAENHIFVTDPDGFRVLEFDQMGNF
                     LRGWGEVSSGIDGFGVPSGIAVAGDERVWVSDAENNFTLGFLLPPLAIPVEEGDVEFP
                     SESSLPQIPRGLSYQAESGLVVNYVEIPVYRLSDDGTEWVPLIPESISAQLAVEVLPE
                     KDANGDWLLRKDGKTQYKWDATGFKWVPADPVP"
     gene            complement(24953..25327)
                     /locus_tag="P5516_03445"
     CDS             complement(24953..25327)
                     /locus_tag="P5516_03445"
                     /inference="COORDINATES: protein motif:HMM:NF015953.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HRT91524.1"
                     /translation="MKRLLLKVLINAGALFLAVWLLRPHIVMQNDAWYAYIVLGAIFG
                     LVNALIKPVVMFVSCPLVVLSLGLATLLVNTLMFLFAGWFGTVLNIGFTIPQQQFYYA
                     FLGALIVSVVSFILSRILDSSQ"
     gene            complement(25374..26360)
                     /locus_tag="P5516_03450"
     CDS             complement(25374..26360)
                     /locus_tag="P5516_03450"
                     /inference="COORDINATES: protein motif:HMM:NF024917.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT91525.1"
                     /translation="MDERKTDPRIRPIEIQRDLPAIADLIETAFAEQMDDEGRDYLRH
                     LRHIAAGAGAFILDGSTPESSTLPFHGYLWEENGRVIGNLTLIPLRKRDHKSYIIANV
                     SVYPEWRGKGIARQLTDRAIAHVREHDGRHIYLQVRADNPPAIHIYQHAGFEEFTRRT
                     SWVFSKNNLHPPILPPGVDVRRRKRADWAQQKDWLQLTYPPEITWNLPYSLPRLRPDF
                     WSWVENLLSARVCRTWSAQKAGRLIGSASYENGYTGSDYLWLASSPAWEDIAIQALLP
                     HVYTRVLKPQRIVVNYPAGRGINGFTACGMKELHTLIWMKMEIPSRLYSLDG"
     gene            26550..28991
                     /locus_tag="P5516_03455"
     CDS             26550..28991
                     /locus_tag="P5516_03455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT91526.1"
                     /translation="MMRFDRFTERAQEAAQRAAEITQRYGHNQIDTEHILLALIEQPQ
                     GVVPQILALIKVDPQILEERLDYILKTSPKASIYGQGAGQVFITPRVKRIMDLANEEA
                     SKLKDEYISTEHLFLAILSERNTPAAKLLETTGLTRDRVMEAIVELRGGQRVTDPQTE
                     SRYRTLDKYSRDLTQQAKEGKLDPVIGRDNEILRVMQILSRRTKNNPVLIGEAGVGKT
                     AIVEGLAQKIASNDVPEILMGKRVISLDLGAMIAGSRFRGEFEERLKASIEEVQRSSG
                     EIILFIDELHTVVGAGAAQGAMDASNMLKPALSRGELQCVGATTLDEYQKYIEKDSAL
                     ERRFAPVFVDEPSPEDTLKMLMGLRDRYEAHHKVHFSDEALSAAVKLSSRYVSDRRLP
                     DKAIDLMDEAAAKLRVALYSMPPELKQAKSEIERLKAEEELAGVERDYERAARMKAER
                     LRLEEQFNQDRDKWESDHKLDEVVDVDDIAQVLAQWTGIPVSQMLEEESERLLKMEDR
                     LHERIIGQDEAIRAVSDAIRRARSGLKDPRRPIGSFIFIGPSGVGKTELAKALAEFMF
                     DDEDALVRLDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVILFDEIEK
                     AHPEVWNSLLQILDDGRLTDGQGRVVDFRNTVLIMTSNLGTEFVRQGGALGFLQKSDT
                     DEDRQAQEKIEKALKSTFRPEFLNRIDEIIMFSALTPEQMLEIVDLQMNEVRSRLEER
                     GLKVELTDEARTWLAKEGYDPSFGARPLKRALQKYVENPLSVTLLSGEYGDGDTVLVD
                     LDKEKQEITFRKQEK"
     gene            29171..30241
                     /gene="gmd"
                     /locus_tag="P5516_03460"
     CDS             29171..30241
                     /gene="gmd"
                     /locus_tag="P5516_03460"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT91527.1"
                     /translation="MKKALITGITGQDGSYLAELLLSKGYEVHGIIRRASTFNTRRID
                     HIYHDPHRNGEEVKLYLHYGDIAAIESLMDVIYNVRPDEVYNLAAQSHVRVSFDMPEY
                     TGDVTGLGTIRLLEAIRRSGNPERFYQASSSEMFGAAKPPQNELTPFEPRSPYAAAKV
                     YSYWVTRNYREGYGIFAANGILFNHESPRRGETFVTRKITRALAAIKAGTQSKLYLGN
                     LDSKRDWGYAPEYVEAMWLMLQQDTPDDYVIGTGEAHSPREFLEEAFGYVGMDWKKYV
                     EIDPRYFRPTEVDYLLSDPAKARARLGWEPKIKFSELVRIMVDADLELSGQKSPGEGK
                     KILTERFNGWHRWDHQVVSMER"
     gene            30332..30916
                     /locus_tag="P5516_03465"
     CDS             30332..30916
                     /locus_tag="P5516_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013618.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRT91528.1"
                     /translation="MIGTQKALVPGIIFSCLLLFLLQQLVKPIGAASAAAEPLRTMET
                     DQATGAEISPQACQFHDRYPAGVVEWCGLIEANAQTYGIDPLLIAAVMAIESGGQPEV
                     ISVSGAVGLMQVMPSDGIAAGFQCINGPCFSSRPTIAELKDPDFNVMYGTRMLAGLIE
                     RAGNARDGLKSYGPADVDYWYADKVIQLYESLNW"
     gene            31454..32062
                     /locus_tag="P5516_03470"
     CDS             31454..32062
                     /locus_tag="P5516_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HRT91529.1"
                     /translation="MPGTIKAVIWDMGGVLLQLVDDRPRQELAKKFSVTLGELDKLVF
                     NSETARLATLGAITEEEHWQQVGKTLGVPPADLRDFQLQFWTGTRVDDRLIEFIQKLR
                     GSYQTALLSNAWSGARKALTTYFDCLHVFDHIVISAEVKMAKPDPAIYLDVLKRLGAE
                     PYQAVFVDDLQENIDTANWLGMHGIVFENSEQAMADVESKLV"
     gene            32040..32651
                     /locus_tag="P5516_03475"
     CDS             32040..32651
                     /locus_tag="P5516_03475"
                     /inference="COORDINATES: protein motif:HMM:TIGR00040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRT91530.1"
                     /translation="MWKANLSDPEEIRIQSEKRIVAAVLADTHVIDRVGRLHPHLIPL
                     IKNSMADLIIHAGDISHPRVLEQLGELAPVYSVRGNRDLYFLRSLPVMQRLNLNGARV
                     VVTHGHLGVAGYWRDKLENTLHGYQFARYYRRLHSLAVNADVVIFGHSHHAENQLREH
                     VLFFNPGSCSVAEKPDYRLSFGVISVSPQAEVKAEIVWLEGHP"
     gene            32870..36787
                     /locus_tag="P5516_03480"
     CDS             32870..36787
                     /locus_tag="P5516_03480"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRT91531.1"
                     /translation="MASVLPTKNYARIPTIFDLPNLIEVQLESFIRLKEEGLEDLFHE
                     ISPIESYNKGMKLYFPGRSPEAKQWGLKYWFEPPKHSIEECVERDLTYASPLYVSVLL
                     AGPDIPEPVKSDIFLGDFPEMTEKGTFIINGTERVVVSQLIRSPGVYFEAPVDRATGH
                     RLAMAKLIPDRGAWMEFETRKNDYLILKFNRKRTVPITIFLRALAAVDDLLPGESPVK
                     TGSDEELIALFSDIDNNPDRQFIPSTIRQEPEWDLTHYQTIAEAALIEFFKRMRPGDP
                     ANLENARQFLQEQLFDSRHYDLERVGRYKLNQKLDLMSRIPVSHRSVTKWDIVYLVRR
                     MILINNEIEDKDDIDHLGNRRVKTNGELIQNKLRIGLRRMERVIKERMSIRDQDQVSP
                     VSLINIRPVVAALREFFGSSQLSQFMDQTNPLAELRSKRTLSALGPGGLRRERAGFDV
                     RDVHHSHYGRICPIETPEGPNIGLIGRLASYARVNEYGFIETPYRKVLKTLAYDDPRL
                     AGRLLRESLVDEKSGEVFAPEGERVTAKMLSAIKKTKKEEIHVAPFVTEEVEYLSADA
                     EDKYVIAQANTILNENNEFAQNQISCRHHSDFILSTPESVQYMDVSPHQVVGISAALI
                     PFLEHDDANRALMGSNMQAQAVPLVNPDSPMISTGMERYAALDSGQVVVAHEDGDVIS
                     VTGQQIVVKNAEGKNDVYHLRKYQRSNQSTCIDQRPAVVKGQRIRKGDILADSSSTVG
                     GKLALGQNVVVAFLSWEGGNFEDAILISERLVQEDKFTSVHIEKYEVEARDTKLGPEE
                     ITRDIPNVGEDAVKDLDEQGIVRIGAEVGPNDILVGKITPKGEKELTPEERLLRAIFG
                     EKSRDVKDTSLRMPHGERGKVVDVKVFTREDNVDLSAGVDMMVRVSVAQRRKITAGDK
                     MAGRHGNKGVVSRVVPVENMPFLKDGTPVDIILNPLGVPGRMNIGQVLETHLGWAAKE
                     LGFEAITPVFDGANEYEIEAELARAWLMDYTWTEAGKRAWEWLNGIDHDPESIKDDDE
                     VRLLYLEQTLDHKYDMGRIATDPTYARQITLESWLNERGFDLAPILDSADPNTEAERK
                     ERDTQAINTCLRVWLEVNGTDPSKIKDEKIREVADKVALETRQPVPTLGKQILRDGKT
                     GQAYDQPVTVGVMTMLKLHHLVEDKVHARSTGPYSLVTQQPLGGKAQFGGQRFGEMEV
                     WALEAYGAAYTLQEMLTVKSDDVQGRVNTYESIVKGEPIEDPSLPASFRVLVKELQSL
                     GLAVEAVMDTKEIIRFGKDEERVRPPRLETGLLGLGEELQGLL"
     gene            36992..37411
                     /gene="rpsL"
                     /locus_tag="P5516_03485"
     CDS             36992..37411
                     /gene="rpsL"
                     /locus_tag="P5516_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003225781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRT91532.1"
                     /translation="MPTINQLIRKGRETKRAKSKAPALQYTLNSMKQRRSRLEKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLSNGIEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDTSGVAKRSQARSKYGSKKPKA"
     gene            37437..37904
                     /gene="rpsG"
                     /locus_tag="P5516_03490"
     CDS             37437..37904
                     /gene="rpsG"
                     /locus_tag="P5516_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HRT91533.1"
                     /translation="MRRSKPEPREVTPDVRYNSVPVTYFIHRIMKRGKKSVATTIMYD
                     ALEMIKEKTGKSPVEVLDLALKNVGPVMEVRPRRVGGATYQVPMEVPAHRRMTLAMRW
                     ILDAATSRTGNSYAEKLSTELIEASNNQGNAVRKREETHKMAEANRAFSHYRM"
     gene            37928..39997
                     /gene="fusA"
                     /locus_tag="P5516_03495"
     CDS             37928..39997
                     /gene="fusA"
                     /locus_tag="P5516_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRT91534.1"
                     /translation="MSRNFPLDKCRNIGIIAHIDAGKTTTTERILFYSGRTYRLGNVD
                     EGTTVTDWMVQERERGITIVSAAVTAEWKGYQINVIDTPGHIDFTAEVQRSLRVLDGG
                     VVVFDAVQGVEPQSETVWRQADRYSVPRICFVNKMDRVGASLEYTLGTIRERLGAQPI
                     QMQLPIGSEAGFKGVVDLLTMQAVYFEDDLGKEPKYAPIPEDLRKQAEDLREQMVEQI
                     VELNDGLTVKYLEGEKISIDELKQALRAAVINTSATPVFCGSSLRNKGVQLVLDAIVD
                     YLPSPVDIPPVIAHSPDGEKEMELPTEDNAPLSALVFKIVSDPYVGRLAYFRVYSGTL
                     KTGDTVLNPIKGRRERIGRLLRMYADRREDITEVTAGDIGAVLGLKESFTGDTLCSVN
                     KPLILESITFPEPVISIAVEPKSTADQDKMALALQRLAEEDPTFKVHTDESSGQTIIG
                     GMGELHLDVLVDRMLREFRVQANVGKPRVSYRETITREVKEIDYKYVKQTGGHGQYGH
                     VVLNLAPLDRGAGVKFESKITGGVIPREYIPAVEKGVKEAVESGVLAGYPVTDLQVQL
                     VDGSFHEVDSSEMAFKMAAILGMREGLQKGASVLLEPIMRTEIVVPEEYLGDVLGQVT
                     SRRGVVLGMDVRPGNAQAIHALLPLSDMFGYATELRSATQGRGVFTMEFDHYAPVSAA
                     VAEKIIN"
BASE COUNT         9479 a         9982 c        11048 g         9367 t
ORIGIN      
        1 tattacgctt tggtgctttt cttcatcggc gcgatggtgg ggctggtgct taccagcaac
       61 ctgctgctca tctttttctt ctgggaaatc accgcgtttt gctcttatgc gttgatcgcc
      121 tttcacaatg atgacccgaa agcagtacgc ggcggcgtca aggcgttgat catcacctca
      181 acaggcggtg tggggttgct ggcaggcgcg ctggtgctct ttgcttacac cgggagtacc
      241 gacatcaacg ctttcattgg cgcggctggc aaaattcccg ccaatatcct ctctctgctg
      301 gcgtttggct tcctggtggc cgcggcggcg aaatccgctc agtttccctt tcagacctgg
      361 ctgccggatg cgatggaagc gccttccccg atcagcgccc tcattcacgc cgcgacgatg
      421 gttaacgcgg gcgtgtacct gctggcgcgc acttaccctg tttttcagaa tgtgccctat
      481 tggcgagaag cggtgatggt tgtgggcacg ctttctgcta tcctggcagc agtgatggcg
      541 ttgaccgcca atgacctgaa aagagtattg gcgtattcga ccgtcagcca gcttgggtat
      601 atggtgtacg ccattggcgc cggcggtgtt ttcgccagtc agtttcatct attcagccac
      661 tccattttca aagccttgct cttccttgga gctggggcgg ttatccactc cattggcact
      721 cgtgatatga cagagatggg aggggtagga aaaaaactgc cgctggtgaa atgggtgttc
      781 ctcatcggtt cactggcgct ggcggggctg ccagtgttga atggattttg gagcaaagag
      841 ctggttctgg atgcgggcct gaaaggcgga cccacctggt tgtatgtttt gatggcgatt
      901 gtggctggcc tcaccgctct gtatactgcg cgttgcgttg ggctggtgtt ctatggcgag
      961 tcgcgcagcg agtatcacgt tcacccggta gggctgggca tgaaggcggc gcttatcccg
     1021 ctggcgacag ccgcgttggt gagctggatt gtggtaggcc cattttctga aatgttgggg
     1081 gctcattcac ttcctttcca tgagattaaa aaaatgccct tggctgagct ctttcatgaa
     1141 attttgaccc tgcccacgct ggttaccctg atcgtaattg ggttgggcat actgttgtgg
     1201 ttcacacggt caaaactgga gggattgacc aacggtttac gcagcatccg ctgggcttca
     1261 gaaaagagtt ttggttttga agccctgaac accgcttttg tcgaggtttt aaataacacc
     1321 gccgaaacgc tgcgcggcac tcaaaccggc attttgaact ggaatgtgct ggcgatttct
     1381 gccgggctgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnc gccttcaggt
     1501 atcagcgcga tgctttcagg ggttgtcatt gaagcgggct tgctggcgat gctgcgttca
     1561 atgagctttt tcggcagggt cgaatttaac tggggggcaa cgctgctggt gtttggctgt
     1621 ttgaatatct tgtttggcaa cctgctggca ctgcgccaga cagaaattaa gcgcctgctg
     1681 gcttattcca gcctggcgca tgtgggttat atgctggtgg gttttgggat gggcttctgg
     1741 tatggacagg caaatggcgc cggcggaggg tttttccacc tgatcactca ttccctgatg
     1801 aaaggcctgg cttttctggc tgccggcgcg ctgctctacg cacttcacct gcagaacgat
     1861 gagcatgtcc ctttgaccct atctgacctt aatggggctt cgcgtaaata cccgttggca
     1921 gcctttgctt taagcgtggc actgctggcg ttgggcgggc ttcctccact ggcaggtttc
     1981 atgtcaaagt ggcaaattct ggccgcggtc gcttccgcga aaaacacgct gatgatactt
     2041 gtggtcattt ttgtggggct gaacagtgtg ctctccctgg ggtattatgc gcccattgtt
     2101 aaccgcctct accgtcgcga aacatcgcct gcggtagaga atggcaaacc cataccggtg
     2161 atgatggtca ttccgctggt gatattggtg ctggctttgg tcgtgatcgg tctgtggccc
     2221 gcgagtatgg atttcttcac cgaaaaggcg acctccagcc tgctgttccc attcttttat
     2281 taggagaccg catacatatg caaatcctta cctccactcc catcgcgctc cttatttacc
     2341 tggcgttagt aacgttgctc tacctggtgg gcaaagccct cgccggtaag gagaacccga
     2401 cgcctgagaa atccagcctc tatggcagcg gtgaagaagc ccctacctcg tttgcctctc
     2461 cgggttaccg cccgtttttt ctcattgcat ttttcttcgc catactgcac ctgggtgtgc
     2521 tggtgatcgg cagcggagaa ttttcattca aactcattcc catcctcctg gggttaatcc
     2581 tcgcgcttgt ggctttgatt ctgggttgat ggaccattac tggagaacag catgaacaac
     2641 cctatccaaa atgtgatgga aaaaataaaa acctgggcaa ggacaaactc accttgggcg
     2701 atccatttta acagcggctc ctgcaatggt tgtgatattg aaattctcgc cacacttaca
     2761 ccgcgttatg atgtagagcg cttcggcatc aagctgcaag gaagcccgcg ccatgcggac
     2821 gtgctcatct gtacaggccc ggtgaccttg caggcgcgtg acaggctggt tcgcatttat
     2881 gaacaaatgc cggagcccaa gtttgtggta gccgtcggct cctgcagtat ttccggtggt
     2941 gtatttgatg gctgttacaa tgtggtcggc gatatcagcg aggtcatccc tgtcaacgta
     3001 tatgtgccag gctgcccgcc gcggcccgag gccatcattt acggaatcga gcagttgttg
     3061 aaaaccctgc agccgtaaaa gtggaggtta gtttgaatac tcaagaagca ttggaaaagg
     3121 ctgaagccat tttaaaagac ctggcactgt cgtttacctg cccggaagaa aaccgccttg
     3181 acgcggttat caaggcagat gatctcaaga aggcggtgga agcgctgttg gtgacgaatc
     3241 gatgggggta tctctcggcg ctgacggggc tggacaaccc tgaatacgaa gtcgatgaag
     3301 acacccggga gaaagtggct gtggaaggga agggttcact ggaggtgctg tatcattttg
     3361 cggctggcgc tgcgataacc actctaaggg tgtcgctgcc ttacgacgca gcggaaatag
     3421 actctatctg tgacctggtt cccgccgcca cgctttatga acgggaagcc atggaattgt
     3481 tcggtatctg ttttaagggc accccaagca cggaacacct ggtgctgccg gattgttggc
     3541 cgaatggcgt gtacccgttg cgaaagactt ttaaaggttt gaacgaaaaa gcgcagggat
     3601 aaggaggaat gagcatgcca agacagaaaa gcgagaggtt tatcgtccca attggcccgc
     3661 agcacccggc cttaaaagaa cccggtcatt ttgaatttac cgtggaagga gaagttgtca
     3721 ccaacgccac tgccaggctg ggctttgttc accgcggcat ggaaaaaggc gcggagagca
     3781 gaacctggac gcaagacctt tacctgatgg aacgggtatg cggcatttgt tcgcatgtac
     3841 actccatggc gttcgtgctg ggggttgaaa aattggccaa tgtgcaggct cccccgcgcg
     3901 cgcaggcgat cagagaactg gtggcagagc tggagcgcat tcacagccac atcctctggt
     3961 tgggagtagc ggcgcatgaa ggcggttttg acacgctctt tatgttctcc tggcgcgacc
     4021 gtgagacggt gatggatctg ctcgaaacca tcagcgggaa ccgggtgaat tattccgcca
     4081 atgtgcttgg cggcgtcaaa gtggatatca acgagcgcca gaaagacgag ctgctgcgcg
     4141 ggatggactt tctcgaagaa cgcacgcggc attatttgaa agtggtaaca gaagatgata
     4201 ccttccttgg gcgcacgcgc aatatcggca tgatgacctt tgaagaagca acccgattag
     4261 gggctatcgg ccctactggc agagcctcgg ggctgttgcg cgatgtccgt atggattctc
     4321 cttatgctgg ctaccctgat tttcccattc aaatcgtcac cgccaaggct ggagatcttg
     4381 aggcacgctt tgtcgtgcgc atcaaagagc tgtttgagag ctaccgcctg gtgcgcgaaa
     4441 ttgtggagaa gattccttct ggtgacctga ccaccaaaat cccacggcgc atccccgaag
     4501 gagaaaccgt gagccgtgta gaagcgccgc gtggagaact cttttactat atcaaaagca
     4561 atggcacaga caggcctgaa cgcgtgaaag tgcgcacgcc cagcctgtgt aattgggcca
     4621 gcgtgttgac caccgctatt ggtcacaaac tggctgatat gcccatgatc ctggcgggta
     4681 tcgacccctg tttttcgtgc aatgaccgta tggtcgtatt gaacgggccc gctggtcaat
     4741 cgctgtggaa ttgggaaaaa ctacgccaat acggaatcga gtactataaa aaatgaatgt
     4801 aacctttcca ccactcatcc ccttgttgct gttccccgga ggattatttc tgctcgcgag
     4861 cggcctagtt tttgaatacg ttgaccgcaa attcatcgcg cgctaccaga accgcattgg
     4921 tcctcgctgg ttccagccgc tggctgacct ggtcaaatta ctggcgaagg aggaagtgat
     4981 tccggcggga acaatcaagg gattgttcat cgctttgccc atcgccgccc tggcagctgc
     5041 gctgacagcc gggttgagcg tgccactatt cgggtttaga ccggttttca gttaccccgg
     5101 agacctgatc ggtaccctgt acttactgag tttgatgacc ctgtgtatcg gattggcggg
     5161 cgccaacacc gaaaaccgtt tttcgatcgt cggcgccacg cgtgctttga cgcagttatt
     5221 ctcatatgaa gcgccttttt tactcgcgct gctcggaccc gcaatcgctt tgggtacctg
     5281 gaacattggc gagatcaacc aggctgccgg gatgaacctg tggttcattc tctcgcagcc
     5341 catcggtttt ttagccgccc tgatcgggtt aatgggtaag cttgaactgc cgccttttga
     5401 cgcccctgaa gcggaaaccg aaattgtggc aggcgcgctg acggaatact ctggtcgcgg
     5461 tttcgcgctt ttcaggattg gccgtgacgt ggaactggtc attgggttga cgctgatcgc
     5521 gtctttctac ctgggcggat tcagctcccc gttagatttt ctatggaaaa cggctgtgct
     5581 gctgcttatc accgccgcgc tgcaatctct gtttgcccgt ttacgtatcg atcaaaccgt
     5641 gggtttgtgg tggcgtgttg gcgccatttt attgttggtg cagatgttgg tgatcattgt
     5701 aggctccagc ctggcgcctt ttgtttcccg gctgtttgga ggttagtatg ccgattggtt
     5761 cgatgttagg tgatattgtt aaatcgtttt tcaaaaaacc ggttactgag aagtacccgt
     5821 tcgtgaaaac cgctgcgcca gaaaacctgc gcggcaaact ggtctggaat ccagaaaact
     5881 gctcgggctg ccagttatgc atcaaagatt gccctgcaaa cgccatcgaa ctgattacga
     5941 ttgacaaggt caataaacgc tttgtgatgc gctacaacct ggacaaatgc acttactgcg
     6001 ctcagtgtgt ggtgagctgc cggtttcatt gtttggaaat gtcagatgac cagtgggagc
     6061 tggctgcgac ccacaaggag ccgttcgagg tctattatgg cagagaagaa gacatcaagt
     6121 tccttctgga aaaagcggct ctcggtggtc ctgaagaagc atgccctgac gcctgacgcc
     6181 agagtcgccg tcgttggtgt gggcgcagaa ctgaacgggg atgacgctgt cggcctatgg
     6241 gtcgccgatc ggctggcgcg cctagtgggt aagcaggaga atttgctggt attgaatggc
     6301 ggttcgctgc ctgagagcac ctctggggcg ttgcggcgtt ttcatccgat gctcgtcctt
     6361 tttgttgacg ctgctgacct ggggaaaaca aagggcgcaa ttgaagtgtt agaaacgcaa
     6421 cgcgttggcg gaacctcatt ttccacacac ggaatgcctt taaagatgtt gatggattac
     6481 ctgggtgaag aactgggttg cgagtctgtg cttatcggcg tccagcctga atgggtggat
     6541 ttcgactcac ctctatcaga ccgcgctaag aaagccgcca accgtttggt aaaagaactg
     6601 taccatttgc tcgcagtacc gaacttggat gaagaagaat tgttggcgaa aaaagaagac
     6661 ctcggagttt gatggcagaa tacaaccagc ggcattccaa agcgactaca atccttgcat
     6721 tgatcccggc atacaacgaa gccgagcgaa tcactgcagt catcacgggt acacgagccc
     6781 tgttgcctgt attggtggtg gatgacggct caactgacga caccgctgcc atctcctcag
     6841 cgttgggagc ggaggtgtta cgccagcttc ccaaccaggg aaagggggcc gcgctgctgg
     6901 cggggttccg ttatgcgctc gaaatgggct accaggcagt ggtcacgctg gatgcagacg
     6961 gacagcatga ccctcaggaa ataccgttgt ttatccacgc ctttgaggag ggcgcggatc
     7021 tggtgatagg catgcgggat ttcaagaaaa tgcccttccc acgcaatatt tccaacacga
     7081 tcggcacctg gttgttctcc tgggcgttgg gacaatattg cccggataac caatccggct
     7141 atcggctgca ttcccgacgc atgatcgaaa cctctctgca aagcgccgag accaactttg
     7201 aattcgaggt tgagattatc acgcgctgcg tggaacaggg ctacctgctt cgctgggtgc
     7261 cgatcaagac gatctacgcc ggcgagaaga gccatatcaa accattacgc cacatccgcc
     7321 atttctttcg cctggtacgc ggcacacgcc accgcctgca gcagctcaag aaacagccat
     7381 gaaagaaccc tttttcacgg gtttcccaaa ccacttatct agtagatcct gcattgtgat
     7441 atagtatgaa tgttttctga aaagtccgca caggcgcaag accggaaccc tttgttgcat
     7501 ctgaatggct gaaggcgaac gaacacgcgc gcgttcctgg gttgtacgat taactttgga
     7561 ttctttacct ggtccatttg caagcatacc tttcagcgag gaaaacagtt aaaaatgcaa
     7621 aacacacgcg aaagcatcga aaaattcttg caattgaaaa aaatttccgt ggtgggggtt
     7681 tcacgtaata aggataagtt tggcaatatg ctctacagag agctgaagaa cagcggctac
     7741 caggtgatgg cggtgaaccc gaacgcagac atggtagaag gtgaccgctg ctacgccggg
     7801 ttgggtgcac ttccagtaaa gcctgaaggt gtggtggtgg ttgtaacgcc agctgaggct
     7861 ttacaggtcg tcgaagaggc ggccggtttg ggtatcactc atatctggct gcagccgggg
     7921 gcgggttcgc gcgaaacaga tcagaaatgc gaggaattga acctcaattg catcagcggc
     7981 gagtgcctct ttatgtatct ggagccggtg aaatcggtgc acgcttttca ccgatttatc
     8041 aggcggttgt tgggcagaat gccaaaataa cagcgctgaa aaagcgagcg gctttcctgt
     8101 cggttctagg cgatttgcgc tttcaattta tcgcgcgttt cgataaactc tcgtaatttt
     8161 gcacgttctt tttccaccac agcaggggga gctttggttg caaaatcact ggcgaggagc
     8221 ttttccaggc ggtccacttg cgcgctcacg acctctaact ctttccgcag ccgttcgaca
     8281 tcctgaacac tgtctgtctt ctccgccagg tcgaggaata tttcgactcc tgataccacc
     8341 actgaaacga atccggttgt atcttccgta atcttgcggc tgatatgaga gtgctcgatg
     8401 tcgagccctg ccagcgcggc aatggtggcg cactggccgc gcagtatatc catcttggcc
     8461 ccggcggaga tcagcgcggg cagcttgcgc ccgggagtga tttttttatc ggcccggatg
     8521 ttgcgaatgg cgcgcaccac ttcctggatt acggtgaagc tggagatggc ttgctcttcc
     8581 caaccttcgc tttttcccgc ttcaggccag gctgcgatga tcagcgcgtc ttcccaggtg
     8641 cgcctggagt tgaagggctt ataggctttg tcaagcgcgg cccctttgag atgaccccaa
     8701 agttcttctg tgacgtaggg ggtaaatgga tggagcaggc gcaagcaggc atctaataca
     8761 cgcaccaggg tggtagcagt gtagtaggcg gcgtcacccc cttgactcat ttggatttta
     8821 gcgatttcca ggtaccaatc cgcgaagtcg ctccagaaga attcgtaaat ctgtcgtccg
     8881 gcttcaccat actgatagga ttggaataag cggtcggtct cgcgtaacag ttcttttaac
     8941 cgaccccaga tccacgagtc tgccagtgtc caatctggct cgctgcccgg ctcagccggg
     9001 gctttttcca cggccgagag gacgaaacgc ccagcgttcc acactttgtt ggcaaaattg
     9061 cggttagctt ccaccttctt gatatcgagg ttggtatcat gcccgggagt tgaacctacc
     9121 agcagggtga aacgcagcgc gtctgtaccg taatcgtcca tgatctccag tggatcgata
     9181 acattgccct tggtcttgct catcttttgc ccatctttgt ccaggattag cccgtgcaag
     9241 tacacggtat ggaagggcac ctgaccggtg aactcaagcc caaacatcat catgcgtgcc
     9301 acccagaaga agaggatatc tgcgcccgtt tccatcaccg aagtcgggta aaagtactgt
     9361 aaatcagcgg tttcttctgg ccagcccagg gttgagaagg gccacagccc ggatgaaaac
     9421 caggtgtcca gcacgtcttc gtcttgccgg atattttgat taccgcattt ggggcaggcg
     9481 gaagggtctt ggcgcgacac gataatttca ccacaatcat cgcagtacca cactgggata
     9541 cggtgacccc accataactg gcggctgatg caccaatcgc gtatgttttc catccagttg
     9601 taatagatct tggtaaagcg ttcagggatg atcctggtgc gtccgtccct caccgcttca
     9661 agcgcttttt cagccagcgg tttgatggcg atgaaccatt gtgttgaaat catcggctcc
     9721 acaatttcac cactgcgctg cgatttcggc acgttcatag tgtagggctg ctctttgatc
     9781 accaaccctg ctgcgcgcat gtcttcccat atctttttcc ggcattcaaa gcggtccatg
     9841 ccctggtagg gaccggcgtt ttcattcata cgcgcggagg tatccaggac gttgatcatc
     9901 tccagcccat ggttcaaacc gatggtgtag tcgttggggt catgagcggg ggtgatcttc
     9961 aacgcgccgg tgccaaactc catggtcaca tattcatcag cgatcaccgg gatctcgcga
    10021 ttcagtaaag gaacgatcac cttcttaccg atgaatcgct gaaagcgttc atcttccggg
    10081 tgaaccgcca cggcggtatc tccgaggatg gtttctggtc gcgtggtggc aaccgggaga
    10141 taagcgcctg aaccatccgc cagcatgtac ttgaaataat acagaaaacc aggttcctcg
    10201 ctgtactcca cctcgaggtc tgaaacggct gtgcgcaaag cgggagacca gttgatcatg
    10261 cgcggtccgc ggtagataag acctttttca taaaggcgca cgaaagcctc gcgcacagcg
    10321 cttgaaagac cctcatccag ggtgaaacgc tcgcgcgtcc agtcgcaaga agctcccagg
    10381 cggcggattt gggaagtgat gcgctggtga taaaggtctt tccactccca ggtgtgcttc
    10441 aggaattctt ctcgggtcat tccatcgcgt tttttgcctt ctttggcgag ggctttctct
    10501 accagcatct gggtggcgat acccgcgtgg tcagtgcccg gaacccacaa tgtagggatg
    10561 cctttcatcc tgtggtagcg gatcatcagg tcttccatag agacaaacat ggcgtgaccg
    10621 gcgtggagct caccggtaac attgggcggg gggattgaga tgacaaaagg ttttatgctg
    10681 gcatcgaaat ccgccttacg tgggtcattt gagggctcaa aatacccttt ttcctcccac
    10741 aggcggtaca acctctgttc ggttgcgcgg aaatcgtacg tttttgccaa tcctgctgtt
    10801 gccattatgt cactcctcaa tccagaataa aaaaaccttt catccgtaga ggacgaaagg
    10861 ctcaatgctt cgcggtacca cctcaattcg ccaatggtct ggcgcactct ccggattatc
    10921 tccggattaa gcgctaacgg gctcacccgt gccgttctac gccctgacgg gtttcttcgg
    10981 catgtattcc aaacgacttc ggtttgtgaa aattgcggag catttcagcc tggagctcct
    11041 tctctaccaa ccttcgctca aacctactcc ttttggcacg gctcgtatag aattatgaaa
    11101 ggattataca aacataacag tcagtcgtca ataagagaaa cgcgatgcct aaaaaagttg
    11161 tacaacacag caagccctcg tggcttacct gggcgcagcg catccaggcg cttgcgcaga
    11221 gcggactata ctacaccagc gacgtgtacg acgcggaacg cttccgcgag ttgagcagca
    11281 tcgcctgtga aatcatggag gcgaacacac tctcacagcc tggtgaagtg cgcatggttt
    11341 tcgcgagcca gagcgggtac gcgaccccca aactggatat ccgcggggtt gttttcaaag
    11401 aggataagat tctgctcgtt cgtgaactgc aagaccaggg caaatggacg ctgccgggtg
    11461 gttgggtgga catcaatgaa ccgccaagtc aggctgtgga gcgtgaagtg cgcgaagaag
    11521 ccggaaagat cgttaaagcg gttaaactgc tagctctgta cgattccaac caacacggtc
    11581 atccccctct tccgtttcat tcgtacaagt tgttcttcct gtgcgagatg ttaggcgaaa
    11641 ccatccccgc tccgtatgag actgctgagc cgtctttttt tgcgcagcaa gaaatccctg
    11701 aactttcaac aacccgggtc acgcatgaag agattcagcg tttgtttgag catttgcgcg
    11761 acccttcgct gcctgccgat tttgattaac cgaaaattcg ggtatactct accttcgcaa
    11821 cctgaggatt ggttgaagga aaagccttga agaaaatact cgttggcgta tgcaaaatgg
    11881 tggtcgtgct ggtcttgagc gtgacggcgt cttgtgcacc gcgctcatct cccacccccg
    11941 cgatcactac aacacccacc gcgcgcatta cctcaacagc caattacagc acaagcactc
    12001 ctaccatcgc tgccatcaat ccccatgtgg atatccagaa actggatggt ttacaaattc
    12061 gtttcatgca cccgtggaca ggggagtcac tcaaactgct tttccatatg gtcgaccagt
    12121 tcaatcaaac gaatgagtgg ggcattcacg tgatcatgca ggctcctggt tcagcggcgc
    12181 gagtgagcga aaagatccat gaagaactca ccaaccagat cgcatccaac gtgctggtgg
    12241 caccggtcag cctgttgctc gccattgatg aacgctttca acaggtggtc gacctgtcag
    12301 gctatatcgc ttctcccctg tatggaatgg atccttcgct ggtcaatgat tttcaccagg
    12361 cattttggga tgagggggtt gtggatggga agctgatggg aatacccgcg cagcgcagtg
    12421 cccttttatt ggcatataac cacacctggg gtcaggagct tggctttact tccatccctg
    12481 atacgccagc ggagttccgc aggcaggttt gcgcggcgaa cgcttcattt agaaaagacg
    12541 ctgatactgc caatgacggg cttggcggat ggatactcaa tactgacgcg gtttccatgc
    12601 tcaactggct gctggttttt ggtgcgaacc cttacagtaa cggtggattc accttttcta
    12661 caacctcatc gcaggatgcg ctgacgtatc tttacggact caaaaccgat gcgtgcgcct
    12721 gggtgaaccg ttctgccgcc gacgcggagc gtttttcaag gcgcgaaacc ctggtttatt
    12781 cgctgtggct gcaggagttg gacaggcagg tggcggctac ggagcgcagc ggttcaaaag
    12841 atgaatggat gttaatgacc tatcctgatg gtatgaaggg gcaagtatta acccagggtt
    12901 cttcgtacgc cgtgctgcat aaagacgctg aaagcgatct ggccgcctgg ctgttcatcc
    12961 gttggctgtc gcaacccgcg caacaattca ggttgttaac cgcctcaggt acactgccgc
    13021 tgggaagcca ggtgatgacg cagatggaag aatacggtgc cgaacatccc cactggctgc
    13081 agatagtgaa ctcgttgacg cgatttacta cccctccggt gaacgctgac tggcaggtca
    13141 tcgcaccggt gttggaggac gccggctggc agttatggat gggcggcacc aaggaagacg
    13201 agattactgg cttgctggca caaatggatg accttgccgt agagcttgct gaaagatacc
    13261 cctgatgaca gatgagatca tcatttacac tgacggcggg tgcgatccga accctggttt
    13321 ggggggctgg gctgcggtga tcattgaggg caaaaaccgc cgggaaattt cgggtgctgc
    13381 agctttttct accaacaacc gtatggaatt gaccgctgcg attgaagcct taaaagtggt
    13441 caaaaagaat cttccaatca ctttgtttac tgattctcaa tatctcaaga gggggattga
    13501 tgagtggcta ccgcgctggc tggcaaaaaa ttggcgagga gccaacggcc cggtggcgaa
    13561 caaagacctg tggcaggaac tgctgcgcgt aacccgcgac cgaaaggtga actggcgctg
    13621 gatatacggt cacacgggca acccggacaa cgaaagagtt gactttttat gccggcttgc
    13681 acgttctcag ttcaggcgtt cgtactgact ccgttaatga tattttgtgc ctggagatag
    13741 acaggctctt cagaaagact gaagaggata tcccctttct tcaactccag gctaccggtt
    13801 ggttggatgt attctccatc tctcaagacc ataatgatga ggtattcttc gggcaacttt
    13861 aactggtaaa tggctttatt gaccgcggga gaggagggcg tgatgagaat ctcacgcagg
    13921 tcggctttta atccctgccc tgaaaccacc tcgatggggg cttgggaatt gacgggcagc
    13981 ggcgcatcca ctttgagcca acacgccacg acgggtaaca gggtgccctg cagcaagacc
    14041 gaggtaatca cgatgaaaaa aaccaggtta aagatggcgc tggcatctga aatgcctgca
    14101 acgagtggaa aagtacccaa cactaccggc accgctccgc gtaaacccac ccaggagata
    14161 aaggtctttt ctctcaaggt gtagcgaaaa ggaattaggg tcaggaaaac cgcgatgggg
    14221 cgcgccagaa aaataagcac aaacgaaacc agtaacccgg ggagaatgac cggcttgatc
    14281 tcagagggga acaccaacaa ccccaaggtg ataaaaaggg ctatttgcat caaccaggcg
    14341 tttccgtcaa agaaccgcac cagtgaacgc ttgtgcagga aatcttcttt gcctaatagc
    14401 agccccaaca cataagccgc caggtaaccg ctgcccccca gaaggctggc aagcccgaaa
    14461 gtcagaaaca ccagcgcgaa aatcaacacg gggtataagc cttcgtaccc cagcttaagc
    14521 cggttaatca aaaagagcga cagccggctc attaaccagc ccacaatcaa gccaactccc
    14581 atctgcataa tgaacaacaa gatcatttca atgggttggt cgatcttgtt ttgaatccat
    14641 tgcaggatgc cgatggtcag gaagatggcc atcgggtcat tactacccga ttcaagttca
    14701 agcaggggag ccaggtttcc tttcaggcgg atcccttttg aacgcagcag ggcaaaaact
    14761 gccgccgcgt cagtggatga ggcaatggcg ccgatgagca ggctcacatg ccagttcagt
    14821 tggagaatga acctggagaa aagagccacc acggcagcgg tgagaaacac ccccagcgta
    14881 gccagcagca gcccttcttt gaacaacggc ttgatgcgtt tccattctgt atcaatgccg
    14941 cccgagaaga ggataacaga gagggctacc gtacaaaccc attgagcgac caatggatcg
    15001 tcaaagtaaa ttccgcccaa tccctctgaa cccgcgacca tacccagcaa gaggaacaat
    15061 aacaacaaag gaatccccag ccgttcagaa accttgctga ccatcacgct gatcaggatg
    15121 aggattgctc cgatgattaa aataatttca gggctttcag gcatgtccca attatagtga
    15181 aagaattctg ttttcgcaaa attaatgaaa cttgaaagcg ctgcactgga gtaataacaa
    15241 ctggggaatg gcggtttcag gcgctctcaa tctaaggggg tgaagacgca tcgcaggtgc
    15301 cttggtcgtt taacgaatta atgaatgagg aggcgacgat gggatttgaa cccatgatca
    15361 gggttttgca gacccttgcc ttagcctctt ggccacgtcg cctcgcaatg aggattttat
    15421 cagaaggtta aagggtggtc aagcagcagc aggctagatg taaagaaaaa agaggtaact
    15481 ttggatgggc aataggaatg agcagcctcc cagagcccaa aataaaaacc ccttttcagg
    15541 ggtttgtctg agcggacgat gggagtgcat agcctcccag agcccaaaac aaaaacccct
    15601 tttcaggggt ttgtctgagc tgtcgatggg agtgcacagc ctcccagagc ccaaaataaa
    15661 aacccctttt caggggtttg tctgagcggg cgatgggagt gcacagcctc ccagagccca
    15721 aaataaaaac cccttttcag gggttnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15781 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15841 nnnnncagaa aggcggaaac gcgcacgctg aagttcatgc ggcggagagg agtatccagt
    15901 tgttgggggg aagtctgcgc aggttaagcc tggtgacgct gcccggggta gtagatgaac
    15961 gttacctggt ggtggtagat aaaaaagccg ccacgccgcc gatatacccg cggcgcgctg
    16021 gaatgccttt gaaaaaaccc ttgtaggtca atctttcgcg ccaagctggg cgacactgcc
    16081 ttccaccacc cgccatcgct cacatttctc tctgaaatca tgcgggaaca ggttcatatc
    16141 ggtggtggtc aacagcacct gttcggcatt acccaggtaa tcgagcaggt aagcgcggcg
    16201 ttgtgtatcc agttccgcga gggtttcatc caacagcaac accggccact gaccagattt
    16261 ctcttttaac caatccacct cggccagctt gagtgaaagc agcgcggtac gtatttgacc
    16321 acgtgaacca aaatcggtga ggtcaatgcc gttactcaac acacgcattt cgtcgcggtg
    16381 cggcccgatt gtggtgatac cgcgggcgat ctcctcggcg cgcaattcgg ccaggcgggc
    16441 gatgaacccc tgtttcactt tttcctggct caaaccgctc ctttgaacct gcacctgcat
    16501 gggcaacgcg aattgacctt gcggcgcggg gagagggtcg tatgaaggtt gataattcag
    16561 gcgcagcact tctttcgaac cagtcaggcg ttcgtgcacg cgcttggcta tttcttccag
    16621 ctcttgaatg acaaccgcgc gcgtggtaat cagcagcgca gcgcgcgccg ccaaaatttc
    16681 atcccaataa tccaactgcg agcgatcgcc gccgcgctcg ttgaggagtt tcagcagagc
    16741 gttgcgttgg gtaagcgcct ggttgtaatc ggcaagggca cgggtataaa ccgggttgga
    16801 ttgggagatg gtcaggttta aatacctgcg tcgctcctcc ggtccatctt cgatgatgcc
    16861 ggtcatttgc ggcagaaaga tgaccgcgtt aaattgccca atcacctgtg cgggaggagt
    16921 cttcacgcca tccaacagca cttctttgcg cagccgcgca ccgccgttcc cgttcgaatc
    16981 ctggatcatg cgcacttcca accggtgcgc gcgttccgct cgtgtatatt gagccaccag
    17041 tcgcgcaacg gccaggtttt ctcgcgtggc gataaagtta agcagttgac ggtcgttttg
    17101 ggcgtgaaat gaggtgaagg tagccagata ataaatcgct tcaaggagcg aggtcttccc
    17161 ctgggcattg gcgcccaaca acaagagaat ccgccggggg acgttcatat ccaggcggga
    17221 aaaaaggcgg aaattcgtca gcgaaatatg ggtaaggtgc ataacctaat caagcgggat
    17281 gtcttcctcc ggcgcgggtt tccagacccg cagcgcgcgg tcagggtata aaactccgtc
    17341 caggtgatcg atttcatgct gaaaaatcct cgccagccag ccgctggctt tgatctttgt
    17401 tggttttcca aagcggttct gaccttttac aataatggcg tgatagcgtt caacttcacc
    17461 gaccagctca ggaacagaaa ggcagccttc gataccgatg accttatctt ctgaagaact
    17521 gataatttcg ggattggcca ccacgaagag cttcttgggt ttttcttcat cttcttcatc
    17581 gccgtactca accacaatga cgcgctgaga ttccgccacc tgcggagccg ccaagcctac
    17641 gcctggagct tctctcatgg tgtcgatcat atcggttacc agcacctgaa aatccctgcc
    17701 aaaatcggtc accttgcggg ctttcttgcg caaaatgaga tgagggatgg tgatgatttc
    17761 tcgtaatgcc atgtaaaatc ctttctacct gttcctgagc aattttaccg ttaacctact
    17821 ggctatcgaa aggatcttta tcttcctttt tcatttcatc ataggccttg atccaatcca
    17881 caagcttttc ctgttccaag cgcagtgttt tttggtctcg atgcttaaac cgtgagaaga
    17941 tttccgcctg gacggctgcc ggattgtaga cgtcatcgaa agacagttcc tgattgccaa
    18001 tttgaatatg taccgtgccg aagttcagta tcaggccaat gatgcctttg cgtttatact
    18061 ccacagtctg gatattctgc accggcgccg aacgccgctc ttctttgcca agtggctttc
    18121 ggttaacatc cacgagctgc tcagaggtga tgatgtaaac gtcgttgtac caatcctggt
    18181 attgatacag ccaccaagcc cagctggtga cggcgaggat gatgctccct atgtacaggt
    18241 ggtctgctgc aatggaagga agaaagccag ccagtttcaa caacaccagt aagaccacaa
    18301 tcagcaacag gagggtgggt aaaaaagttt ttcccagcag ccgccaccag tgtgtgcggt
    18361 aaataatcgc ccccgcgctc tcgtggcgca atccaaaaaa ccgcgctgcc agatccagaa
    18421 aagaaccgct tttataagtc gtttttaatc ctcctgtatg cttaccagcg gcagcgtttt
    18481 tcgtcctgac cgttgctcct tccagccgat gctgcaaggt ttcgcgcatt tcagcgtaat
    18541 cgctctgatg ctttttctgg gcaatgctcc tgcgttgata ttccagcaaa tcgaaaataa
    18601 tctcggggac aggcagacgt ttaaagctga gcttaccatt gtaggttctc aagttgatcg
    18661 tgccgaatct cagtagcctg ccccagaaag atgtttcaaa gccggtagaa aggatggcag
    18721 tgtagggagc ttcctggcgg ttgtcgaaaa aaccagttag ttttttttgc acgagcaccc
    18781 ggtcagaagt cagtataaaa aagtcattgc cccattccag ggcagaccac gcggtcagca
    18841 aagtgccgat caagagcagc gtgccggcga gcaggattaa ggccaccgat ttgctggaca
    18901 gcgccagcga tagcaaacca gcgaaacttg caattaccgc gccgccaatg agtaccacgc
    18961 gcagtgcgag aaagaatggg tgccgacggc aaaccaggtt gactttttcc cctttctcca
    19021 gccaggggaa aaagaggcgc gcgcggaatt tgtgggtctg gtagatcagc gaaaacgcgt
    19081 tttctatggt tggaaggtta gcgcacaact gaatgaggtc ttgctcatta actctgacca
    19141 gctttacctc gctgagacag acagcatctg aaaagcgata atcgctgcga gaaagcactt
    19201 ctgcgccgaa ataatcgccg gcgcccagca cctgctcagc tttaccgcgc cgcatggcgc
    19261 cccctgccag ccgtacatta ccggaaatga tccaataaaa ataatctgga atatcatcag
    19321 cagcgtatag ccggctgccg cgcgggtaat tcagtacctc aaattgggca gccacctggc
    19381 taaggtcgtt cccttccaac aatgtgaggg gaaataaccc cgctaaaagc gaaaccgttg
    19441 caggctcaac tgtttccata ctataagtat aactaaagct caaaaaaaag cgtggttttt
    19501 cagcaaaaag gggggtgcca aagtctggta aaatttccat ggcatgtacg gaaactgatt
    19561 taatgatgaa gcgcgttggg catttttcga tatttcgctg gctgtcgctg gcttttattt
    19621 tggctgcgct ggtgttgacc gtatttcaac ttgtggtttt cagccggttg cgctcatctt
    19681 tttccacggg aaccagcatt gcaggtgtgg atgttaccgg cttgtcgatg gaacaggcag
    19741 cttcacgcgt gacacaggcg tattccattc cagtagaact gcgttataac aacagcgtca
    19801 tccaagttaa gcctggcacg ctgggatttt ctttagatat gactgcgatg atgaccgcgg
    19861 cagatcagtt gcgctcggcg gaacctttct ggccgtcctt catcgcttac ctttttaacc
    19921 agcagccttc ggccagcgag ataccattgc gagcagaaat cgatgacaag cagctgcgcg
    19981 cttacctggt gaatgagatc gccagccgtt acgatgaaga acctgcggct tatttacctg
    20041 tgccggggag cgtcaccttt gaagccggca cgccaggcaa gaaactggat gtggaccgtt
    20101 ctgcgctgct ggtatcgaac gcgctgcgct ctcctgctgc acgtatcgtc aacctcgcgc
    20161 tgattaccgg taagctgaac cgcccttcta tggataatct acgtatcctg ttgcagcaaa
    20221 ttgtagatgt gaataatttt gacggcgtgg tggaacttta cctgcaggac ttgaaaaacg
    20281 gtagcgagat gcaaatggct taccagaatg gcgaatccct cactcctggc atagcgtttt
    20341 cagcggatag cactatcaaa atcgccgtaa tggtcaccgc ctaccaagag gtggctgaac
    20401 ctgccagcac gaaagtggta caactgctcc agtctatgat tgccatgtca gacaacacct
    20461 ctacagacaa cctgatgcgt gaagtcctct cgccaaccct tggtccactg actgtgacca
    20521 gcacgatgcg caaactggga ctgcaaagca cttttctgga tggcatgttt tacattggcg
    20581 cgccgctgct tacaggaaac attgtcaccc ccgctaattc tcggcgtgac gtttttaccg
    20641 accctgatcc atataaccag accaccccta ctgaaatggg tttgttattg gcggatatct
    20701 atcagtgcgc gcagaatgac ggcggcagcc tggtggcggt attcccgggt gaaattaccc
    20761 ccgctgaatg ccggtcgatg atcagcttac tcacacagaa ccgaattggc gtgttaattg
    20821 aagccgggct tccggacggc acccagatcg ggcacaaaca tggctgggca attgatgcaa
    20881 tggatggcgt aatgcatacg gtgggtgatg ctggcctggt gtacacccct ggcgggaatt
    20941 atgtgctggt gatcttcatt tacgaccaga accagatcgt ttgggataat gcgaaccagc
    21001 tattcgcaga cctgtcaagg gcggtttaca attattacaa tttaacagca gaatgaactg
    21061 gatattgttt cagacgttat aaaaaaaaag cgaatgaaaa acacgtcttg tcagtaattt
    21121 ctgaaacaaa tgttgtgagg tatatttttt gcagacagag gatcagtttt gaatatgaac
    21181 agcgctgaaa atgtggaaag caaacccggc tggttagacc gtccgctcta cacctggata
    21241 cccagaatta ccattgaaca cattgtaatt gcgctgatca tcctgatggc tgtgctgtct
    21301 cgtttttacg cagttggttt acgcgtgatg agccatgacg aggttaacca tgtggtgcct
    21361 tcgtatgaac tttatcaggg gcgggggtac gcacacgacc cggtgacgca tggtccgctg
    21421 caatttcacc tggtggcggg ctcctatttc ctttttggcg attctgattt ttcttcgcgc
    21481 atcccggcgg ctttgttcag tattgccacg atcatttttg tgatatttgg attccgcagg
    21541 tacctgggca gggttggcgc gctgctggca ggcgccttct tcctggtatc accttacatg
    21601 ctgttctatg ggcgctacac gcgtaacgag gcttttgtgg cgttgttcgg cgtcgtgatg
    21661 ctttacgccg tgctgcgtta cctggaagac ggccgccacc gccatctcta cttatttaca
    21721 gtggctctgg cgcttcattt caccgcgaag gaaaccgctt ttatttacac cgcccaaatc
    21781 ctcatcttcc tggcggtgct gttcattcga gacgtggtga agcatgaatg gccgcaagga
    21841 ggaaggcgcg atttcttcat tttttcaatg atggcagggc tgctctttgt tggtaccgcg
    21901 ttgggcgcgg cggtgatgga tgccagtatc aaacctggta acgcggaggc tgtggctgct
    21961 cctgcggtgt atcacacagt catgttaatc agcctggcgc tggcggcgct gggcatgatc
    22021 ttggccattg tggtcttgct tgggtcgatg ggctggcgcg tggtcaggga gatccgttct
    22081 tttgatctgc tcattctgac ctccacaatg atcctgccgc aattgatcgc ttttcctatc
    22141 aacctgctgg gttggaaccc gctggattat tctcaaacag ggatgattcg caccttgtta
    22201 ttcttgctgg gagcagtggt catttcagtg acgctgggat tgctctggaa accgcgcctg
    22261 tggctgatca acgtgggtgt tttctatgcc atcttcacga ttttttacac aacttttttc
    22321 accaacgggc agggattttt caccgggatg gtaggatcgt tgggttactg gctgagccaa
    22381 cagggggtta accgtggttc gcaaccctgg tattttttcg cctttctgca ggtgcccatg
    22441 tacgaatacc tgccgttggt gggtacgcta atcgcggtcg tcattggggt caagcacagg
    22501 cttttttcca gcctgcccgg tgttgcgccg gcgcttcaaa ccgcggagca ggttgaggtt
    22561 cccagtcagc tcccactgcc agcactggaa ggcagtgaag aaacaccagc agaaaagcca
    22621 ggcaaattac cggtgttgag tttattggtg ttctggggat tgaccagcct ggtggcattc
    22681 acgctcgcag gtgaaaaaat gccctggctg accgtgcacg taaccctgcc tttgttattg
    22741 tgcgccgggt ttggctttgg ctacctggtg gattccactc ccttcaaaca actggccaac
    22801 aaaaaagggc tgcttgtcct tgcgctgctg ccggtgttca tcaccagcct cggcgggacg
    22861 attgccgcgc tggtgggcac ccaaaaaccc ttcgccggca aagagctgga gcagttgaag
    22921 gcaacgagca ccttcctttt tgccttgatc gccactctgg catctggcgg gctcttgtgg
    22981 aaattgctgc tgcgctggag ccagcgggcg attctcaagt tattgacgct gactttgggc
    23041 gcgatcctga tggtgcttac cgcgcgctcc gcgtaccagg cgagttttat caactatgac
    23101 actggcaaag agtttctcgt ttacgcacac gccgccaggg gaccgaagga tgtgctggag
    23161 caggtggagg atatttcgca gcgcaccact ggagggaagg acatccgcgt ggcttatatc
    23221 ggggacgcgc tctaccctta ctggtggtac ttccgcgatt atcccaacaa ggtctggctg
    23281 aaggatgacc tgacgcggga cctgttgaat tacccggtgg ttatctctga cgatgagcat
    23341 tattcaaaaa ctcaggccat actgaaagac cagtattttg aaaccaaata caagcggctg
    23401 gtctggccga tgcaagatta cttcaatatg acctgggaac ggttctggaa gggtttcacc
    23461 aatccccaga tgcgccaggc aattattaat atctggctga acaaagatta ccgactgtac
    23521 gcgtcgcttg ccaataatag tggtttgacg ctggagaact ggcagccttc ggcaaatatc
    23581 taccttttta tccaaaagga cattgtcgag cgcatctgga cgtatggcgc tatcccccag
    23641 cagcaggaaa cagcagaaac cgacccgtac gcggggaagt attttgaatt aacgcctgat
    23701 agggtttttg gcagcagcgg cagcgctgaa ggacaactga ccatgccgcg agggttggcg
    23761 gtggcagcgg atggatcggt ttatgtggca gacgccaata atcaccgcat tcaaaaattc
    23821 agcgcggatg gcgcgtttct gctctcgtgg ggaagttacg ccacagcgga aggcggcaac
    23881 gccccaggcg gtaccttcaa tgagccgtgg ggcgtggcag ttgcccccga tggctcagtg
    23941 tacgtggcgg atacctggaa tcaccgcatt cagaagttta cccgtgatgg caaattcctc
    24001 accatgtggg gagttcccgg cttggcggaa gaaccggacc gcttctgggg tccgcgcggc
    24061 atcgcggtgg atcgcgaagg gacggtgtat gtgacagata cgggtaataa ccgcgtggtg
    24121 atttttgatg aaaacgggaa ttaccagcaa cagtttggcc gcagcggcat caaccccggt
    24181 gagttcgatg aaccagtcgg tatcgcggtg gatggcaacg gacgggttta tgtggccgat
    24241 acctggaatc agcgcatcca ggtttttgaa cccgccggca aagaaggtta cagcgtgctc
    24301 aggtcctggg atgtgcatgg ctggtttggg cagtccatca acaataaacc gttcctggcg
    24361 ctggacgcgg aaaaccatat ttttgtgaca gacccggacg gctttcgtgt gctggagttc
    24421 gaccagatgg gcaacttctt gcgcggctgg ggagaggtta gctcgggcat cgatgggttt
    24481 ggcgtaccca gtggcatcgc cgtggctggt gatgaacggg tttgggtgag cgatgccgaa
    24541 aataacttca cccttggttt tctcctgccg ccactcgcga tccctgtcga ggagggcgat
    24601 gttgaatttc catctgaatc cagcctcccg cagattccgc gtgggctatc ttatcaagca
    24661 gagagcgggc tggtggtgaa ttatgttgaa atcccggttt atcggctaag cgacgatgga
    24721 acagagtggg tgcccctgat tccagaaagt atctctgctc aactggctgt agaggtctta
    24781 ccagagaaag atgccaatgg tgactggctg ctgcggaaag atgggaaaac gcaatataaa
    24841 tgggacgcga caggttttaa gtgggttccg gctgaccctg ttccttaatg gataagaaac
    24901 gaaaaattaa cagcgcctca ctggcgctgt ttttatcatg atgaagatca gcctattgag
    24961 aactgtctag aatgcggctg agaatgaagc tgaccacgct gacgataagc gctccaagaa
    25021 acgcgtaata gaattgctgc tgcggaatgg tgaagccgat gttgaggacc gtaccaaacc
    25081 agccggcgaa aaggaacatc aacgtgttca ccagcagtgt tgccagcccg agcgaaagaa
    25141 ccacaagcgg gcaactcacg aacattacca cgggtttaat gagcgcgttg acaagaccga
    25201 atattgcacc gaggacgatg tacgcatacc aggcatcatt ttgcatcaca atatgcggac
    25261 gcaataacca cacagcgagg aaaagagcgc cagcgttgat gagtaccttc agcagtaatc
    25321 ttttcatatg agtttccttt cgcccataca tacgcaggcg agggaaaaaa gtttcatcca
    25381 tccagactgt acaacctgga gggaatctcc attttcatcc agattagggt gtgtagttct
    25441 ttcatgccgc aggcggtaaa tccgtttatt cctctgcccg cggggtaatt taccacgatg
    25501 cgttgcggct tcaataccct ggtgtataca tgtggcagta acgcctgaat cgcgatatct
    25561 tcccatgccg gcgaactcgc cagccatagg taatcagagc cggtataacc gttctcgtag
    25621 gaggcactgc cgatcaagcg gccggctttt tgggcagacc aggtgcggca cacacgcgcg
    25681 ctcaacaggt tttcaaccca agaccagaaa tcagggcgca gacgcggcag gctgtaaggc
    25741 aggttccagg tgatctctgg cgggtaagtg agctgcagcc agtctttctg ctgcgcccag
    25801 tccgcgcgtt ttcttcttct cacatcaacc cccggcggaa ggattggagg gtgaagattg
    25861 ttttttgaaa agacccagga cgtgcgccgg gtgaattctt caaaacctgc gtgttgataa
    25921 atatgaatgg caggcgggtt atctgccctc acctgcaggt aaatatgccg accatcatgt
    25981 tcacgcacat gggcaatggc tctatcggtc agttggcggg caataccttt cccgcgccac
    26041 tcgggataca cagatacatt ggcgataatg taagatttat ggtctctttt tcggagggga
    26101 atgagcgtga ggttgccgat aacgcggccg ttctcctccc acaagtagcc gtggaaaggc
    26161 agggtagagc tctcgggtgt acttccgtcc aggatgaacg cgccggctcc tgccgcgatg
    26221 tgacggaggt ggcgaagata gtctctgccc tcatcatcca tctgctcggc gaaagcggtc
    26281 tcgatcaggt cagcgatcgc tggcaagtcg cgctggattt caattggccg gattcgtggg
    26341 tctgtcttgc gttcatccat gccataaatt ttacactgtc tttaaagaac ggcgtgaacc
    26401 ttgcacctgg ttctatcagg gctgcacaga acaatgcgaa ttactatggg tgaaaaagcc
    26461 taaccgaatt cttataaaaa tctttgattt gtttgatatc ctcgatgcta taatgacctc
    26521 gaggaaggac gattgattcg gagtgaatta tgatgcgatt tgataggttt acagaaagag
    26581 cgcaggaggc tgctcagagg gctgccgaga tcacccagcg ctatggacac aatcagattg
    26641 atactgagca tatcctgttg gcgctgatcg aacaaccgca aggagtagta cctcaaatcc
    26701 tggctcttat aaaagtggat ccgcaaatcc tggaagaaag gctggattat atccttaaaa
    26761 ccagcccaaa agccagtatt tacggtcagg gcgccgggca ggtattcatc accccgcgcg
    26821 taaaaaggat catggacctg gcgaatgaag aagccagcaa attgaaagat gaatacatct
    26881 caaccgaaca cctgttcctg gcgatactga gtgaaaggaa tacgccggct gccaagctac
    26941 ttgaaacgac aggcctcacc cgtgaccggg taatggaagc cattgttgaa ctgcgcggcg
    27001 ggcaacgtgt taccgatcct cagaccgaaa gtcgttaccg cacactggat aaatactctc
    27061 gcgacctgac acagcaggcg aaagaaggca aacttgaccc cgttatcggt cgcgataatg
    27121 agattttgcg tgtgatgcaa attctctccc ggcgcactaa aaacaacccg gtgttgattg
    27181 gtgaagcagg tgtgggtaaa acagccattg tagaagggtt ggcgcaaaaa atcgccagca
    27241 atgatgtgcc tgaaatttta atggggaagc gcgtaatctc tcttgatctt ggtgcaatga
    27301 tcgcgggttc gcgttttcgc ggcgagttcg aagaaaggct taaagcgtca atcgaagaag
    27361 tacagcgttc ctccggcgag atcatcctgt ttattgatga gctgcacacc gttgttggag
    27421 cgggggctgc ccagggcgcc atggacgctt caaacatgct caaaccggcg ctgtcgcgtg
    27481 gcgaactgca atgtgtgggt gctactacgc tggatgaata tcaaaagtat atcgaaaaag
    27541 actcggctct agaaaggcgc tttgccccgg tgtttgtaga cgagccttct ccagaagata
    27601 cgttgaaaat gctgatgggg ctgcgcgatc ggtacgaggc acatcacaag gtgcactttt
    27661 ctgatgaagc gctttcagcg gcagtaaaac tgagttcgcg ttacgtcagc gacaggcgtc
    27721 tgccagacaa ggcgattgac ctgatggatg aagcggcagc gaaattaaga gtggcgttgt
    27781 actctatgcc gcctgagctg aaacaagcca agtctgaaat tgaacggttg aaagccgaag
    27841 aagagctggc gggtgtagag cgtgattacg agcgcgccgc gcgcatgaag gctgagcgcc
    27901 tgcgccttga agaacaattc aaccaagacc gggataaatg ggaatcagac cataaactgg
    27961 atgaagtggt ggatgtggat gacatcgccc aggtgctggc gcagtggact ggtatcccgg
    28021 ttagccagat gctggaagaa gaatcggaga gactgctgaa gatggaagat cgcctgcatg
    28081 agcgcatcat cgggcaggat gaggccattc gcgcggtatc ggatgcgatc cggcgggcgc
    28141 gttcagggct aaaagatccc cgccgcccga tcggatcatt cattttcatt ggtccatcgg
    28201 gtgtcggtaa aactgaactg gcaaaagcgt tggctgagtt catgtttgat gatgaagacg
    28261 cgctggttcg cctggacatg agcgagtatc gtgaacaaca tacggtttca cgcctgttcg
    28321 gtgcccctcc gggttatgtg ggttatgagg agggtggtca acttaccgag gcggttcgcc
    28381 gccgcccata ccgggtaatc ctgtttgatg aaattgaaaa ggcccatcca gaagtgtgga
    28441 actcattgct gcagattctg gatgatggac gactgacgga tgggcagggc agggtggttg
    28501 acttccgcaa cacggtgctc attatgacca gcaatctggg aactgaattt gtccgacagg
    28561 gtggagcgct tggcttttta cagaaatcgg atacggatga ggaccgtcag gcgcaggaaa
    28621 agatcgaaaa agcgctcaag agcactttca ggccggaatt tctgaaccgc atcgacgaga
    28681 ttatcatgtt ctcagcgctt acgcctgagc agatgctgga gatcgtggac ctgcagatga
    28741 atgaagtgcg atcccggcta gaagagcgcg ggctgaaggt ggaattgact gacgaagcgc
    28801 gcacctggct cgccaaagaa ggctacgatc cttcgtttgg cgcccgccca ctgaaacgag
    28861 cgctgcagaa atacgtagaa aatccgctat cggtaacatt gctttcaggt gaatatgggg
    28921 acggtgacac cgtgttggtg gatctggaca aagaaaaaca agaaatcact ttccgtaagc
    28981 aagaaaagtg acaaccgaaa aagccccggg caggtgatag agacccgggg ctttctattt
    29041 aaaaagcatc tgaaagctga tggcgcgcca ggcggcttac cacaaagaaa ccgactttct
    29101 ttcggttata attacccact ggcaagccag atagagcaag aataataact cgacgccatg
    29161 gaggcgaagc atgaaaaagg cattgatcac tggcatcacc gggcaggatg gttcctacct
    29221 ggctgagctg ttgctctcaa aaggatacga agttcacggt atcatccgca gggcgagcac
    29281 cttcaacacc cgccgtattg atcacatcta ccatgatccg catcgaaacg gtgaagaggt
    29341 gaagctctat ctacattacg gagacattgc ggctatagag agcctgatgg acgtgatcta
    29401 taatgttcgc ccagacgagg tctataattt ggcggcgcag agccacgtgc gggtaagttt
    29461 tgacatgccg gaatacaccg gggatgtgac cggattgggc acaattcgcc tgcttgaagc
    29521 catccgccgc agcggcaacc cggaaaggtt ctaccaggcg tcctccagcg aaatgttcgg
    29581 ggctgccaaa ccgccgcaaa atgaattaac cccgtttgag ccgcgcagcc cttacgcggc
    29641 agccaaggtg tattcttact gggtcacgcg caactatcgc gaaggttatg ggattttcgc
    29701 tgccaatggc atacttttca atcatgaatc cccgcgacgc ggcgaaacct ttgtcacccg
    29761 taagataacc cgcgcgctgg ctgcgatcaa agcgggtacg cagagtaagc tctacctggg
    29821 caatctggat tcaaaacgag actggggtta cgcgcctgaa tacgttgaag ccatgtggct
    29881 gatgctgcaa caggacaccc ctgatgatta cgtcatcggt acgggcgaag cgcacagccc
    29941 gcgcgagttt ttagaagaag cctttggcta tgtgggcatg gattggaaga aatacgtgga
    30001 gatcgaccca cgctacttca gaccaaccga agttgactat ctgctttcgg atccggcaaa
    30061 agcgcgcgcc aggctggggt gggagcccaa gatcaagttc tctgaactgg tacgtatcat
    30121 ggtggatgcc gacctggagt tgagcgggca aaagagcccg ggagaaggca agaagatatt
    30181 gacggagagg ttcaacggct ggcaccgctg ggatcaccag gttgtcagca tggaaagatg
    30241 agcaccgcca ggcgggaacc cccgcctttt taattttgac aaaatagaac aaaagtgcta
    30301 tcataaagaa aaagttcagc ggaggttcat tatgattggc acgcaaaagg cgctggttcc
    30361 tggcatcatt ttcagttgcc tgcttctgtt cttactgcag caactggtaa aaccgattgg
    30421 cgcagctagc gccgcagcag aacccttaag gaccatggag accgaccagg ctactggcgc
    30481 agagatttcc ccccaagcct gtcaatttca tgatcgttat cccgcagggg tggttgagtg
    30541 gtgtgggctg attgaagcca acgcgcaaac gtatggtata gacccgttgc tgatagcggc
    30601 ggtgatggcg attgaatccg gcgggcagcc ggaggttatc tccgtttctg gcgcggtggg
    30661 gctgatgcag gtgatgccca gcgacggcat cgctgcgggt ttccagtgca ttaacggtcc
    30721 ttgtttttcc tccaggccca ctattgctga attgaaagac cctgatttta acgtgatgta
    30781 cggaacacgt atgctggctg gtttgatcga gcgcgcgggg aacgctcgcg atggattgaa
    30841 atcctacggc cctgccgacg tggattactg gtacgcggat aaggttatac aactatatga
    30901 aagccttaac tggtgagctg aaggctgact attgaggagg atcattatca ggctgtcaaa
    30961 tattcaagag attgaatggt tattgattta gaacagtaac atacctcttc agtgaaggcg
    31021 ctcaggcatt taggagtgtt gtcacttaaa agagaaggca actgcaagtg ccaaaaaaac
    31081 ctcaagagat gtgcgtgcca gtatggttta tcaccctgca aacaaaccat ttttttgcgt
    31141 gcttcgcagc gcagacgatg ctgtcttttc gaattgagat gcgcctgcta ttggatagcc
    31201 gccatatcac tgcgctcagt ggttacgggt gcaatgcggt tccgtgactt ttcctaaatg
    31261 agcacctggg cttgattgac ctgggagtct tcctgcgcga ttgggcattg ttataacggt
    31321 tctcgttgga ataaaccagg aaaaacgtgg cgaacgagtg aacaattgga gatagaggtt
    31381 gttacgttgt tcccgcagct ctccgatttt cccgtgttat actctttttc aatatctaat
    31441 aaagaggaga aaaatgcctg gtacgatcaa agccgtcatt tgggatatgg ggggagtgct
    31501 gttgcagctg gtcgacgacc gtccgcggca ggaactggca aagaaattca gcgtgaccct
    31561 gggtgaactg gataaactgg ttttcaacag tgaaacagcc cgccttgcga ccctcggcgc
    31621 gataaccgag gaggaacact ggcaacaggt agggaaaacc ctcggcgtcc cgcctgctga
    31681 tttacgcgat tttcagcttc aattttggac cggcacccgg gtggatgacc ggttaattga
    31741 gttcatccag aagttacgcg gcagttacca gaccgcgttg ctaagcaacg cctggtctgg
    31801 cgcgcgcaag gccttgacaa cctattttga ttgcctgcat gtttttgacc acatcgtcat
    31861 ttcggctgaa gtgaagatgg cgaaacccga ccccgcaatc tatttggatg tactcaaacg
    31921 cttgggagct gagccatatc aagcggtttt tgtggatgac ctgcaagaaa atattgatac
    31981 cgccaactgg ttgggtatgc acggaatcgt ttttgaaaat tcagaacagg cgatggcgga
    32041 tgtggaaagc aaacttgtct gaccctgagg aaatacgaat tcaatcagaa aaaaggattg
    32101 tcgcggcggt gctggcggat acacatgtga tcgatcgtgt gggcagactg catccacacc
    32161 tgatcccatt gattaagaac agcatggcgg acctcatcat ccacgccggt gatatttcac
    32221 accctcgtgt tctggagcag ctgggcgaat tggcgccggt gtattctgtg agaggaaacc
    32281 gcgaccttta ctttctccgt tcactgcccg tgatgcagcg gctcaattta aatggtgccc
    32341 gggtcgttgt cacccacggg cacctgggcg tggcaggtta ctggcgtgat aagctggaaa
    32401 ataccttgca cggttaccag tttgctcgtt actacaggcg actccattcg ttggcagtga
    32461 acgcagacgt ggtcatcttc ggtcattcac accatgcaga gaaccaactg cgggagcatg
    32521 ttttgttctt taacccgggt tcgtgcagcg tagcggaaaa acctgattac cggctgtcgt
    32581 ttggcgtgat aagtgtctcc cctcaggctg aggtgaaagc agaaattgtt tggcttgagg
    32641 gtcacccgtg atcagccgac gggagcggga gaggtgaaat acgcgttaat acgcaaaggc
    32701 aaagcgcata taaactgtag acgatgtgct gaccccccta aatgaactaa cacgaattcc
    32761 ttgaccattg aaaaacgcgt tggtataatg acaagttgcg catcccggct ctggaatggg
    32821 ctgggagttt attcgattat cgtcggtttt gacattttag gagaaaccaa tggcttctgt
    32881 gttgcccact aaaaattacg caagaatacc aaccatcttt gacctgccca atttgattga
    32941 agtccaactt gaatctttca taagattaaa ggaagaaggg ctggaggatc tctttcacga
    33001 gatctcgcca atcgaatcct ataacaaggg gatgaaactg tacttccccg gccgcagccc
    33061 tgaggcgaaa caatggggac tgaaatactg gtttgaaccg cccaagcatt ccattgaaga
    33121 gtgtgtcgaa cgtgacttga cgtacgccag cccgctgtac gtttccgtgc ttctggctgg
    33181 ccctgatatc ccagaaccgg tgaagtcaga tatcttcctg ggcgatttcc ccgagatgac
    33241 cgaaaaagga accttcatca tcaatggtac tgaacgtgtg gttgtatcgc agcttattcg
    33301 ttcgccgggc gtttactttg aagccccggt agaccgggct accggtcatc gcctggcgat
    33361 ggccaagctt atcccagacc gcggtgcctg gatggagttt gagacgcgca agaatgatta
    33421 tttgatttta aagttcaacc gcaagcgcac cgttcctatc acgattttct tacgcgcgct
    33481 ggcagcggtt gatgatctgc ttcctggtga atcaccggtc aaaactggct ctgatgagga
    33541 gctgattgca ttgttcagtg acatcgacaa taacccagac cggcaattta tcccttccac
    33601 tatccgccag gaacctgaat gggatctgac gcattatcag acgattgcag aggctgcgct
    33661 gattgagttc tttaaacgta tgcgccctgg cgacccggct aaccttgaga atgccaggca
    33721 atttctccaa gagcaactgt ttgacagccg ccattatgac cttgaacgag ttggccgcta
    33781 taagttgaac cagaagcttg acctgatgag ccgcatcccg gtttctcacc gcagtgtgac
    33841 caagtgggat atcgtctacc ttgtccgccg gatgattctg atcaacaatg aaatagagga
    33901 caaggatgat atcgaccacc tggggaaccg tcgggtaaag accaatggcg aactgataca
    33961 aaataaactg cgcatcgggc tgcgccggat ggagcgggtg atcaaagaaa gaatgtcaat
    34021 ccgcgatcag gaccaggttt cacctgtcag cctcatcaac atccgcccgg tggtcgccgc
    34081 gctgagagag ttcttcggct cttcacaact ctcgcagttt atggaccaga ccaacccact
    34141 ggctgagtta cgcagcaaac gcacgctctc tgccctgggc cctggcggct tgcgccgcga
    34201 gcgcgctggc tttgacgtgc gagatgttca ccactcgcac tatgggcgta tttgtcccat
    34261 tgaaacacct gaaggcccga acatcgggct tatcgggcgt ctggcgagtt acgcgcgcgt
    34321 gaatgaatac gggttcatcg aaacccctta tcgtaaggta ttaaaaacgt tggcatatga
    34381 tgaccccagg cttgcaggac ggttattgcg cgagagcctg gtggatgaaa aatcaggcga
    34441 ggtcttcgcc ccggagggtg agcgcgttac cgccaagatg ctttcagcga ttaagaagac
    34501 gaaaaaagaa gagattcacg ttgcaccttt tgttactgaa gaggttgaat acctgtcggc
    34561 tgacgcggaa gataaatacg tgatcgcgca ggcgaacact attttgaatg aaaacaacga
    34621 attcgcgcag aaccagattt cctgccggca tcacagtgat tttattcttt caacccctga
    34681 aagcgttcaa tacatggatg tctcgccgca tcaggtggtg ggtatttccg ccgcgttgat
    34741 tcctttcctg gagcatgatg acgcgaaccg tgcattaatg ggctcgaaca tgcaggctca
    34801 ggcggtgccg cttgtcaacc cagacagccc catgatttct accggcatgg agcgttacgc
    34861 cgcgctcgat tctggtcagg ttgtggttgc tcatgaagac ggcgatgtga tttcggtcac
    34921 cgggcaacaa atcgttgtga aaaacgctga aggaaaaaat gacgtgtacc atctacgcaa
    34981 gtatcagcgt tccaaccaga gcacctgtat tgaccagcgc ccggctgtgg tcaaaggcca
    35041 gcgtattcgc aagggagata tcctggcaga ttcttcctcc actgtcgggg gcaagctggc
    35101 gttgggtcag aacgtggtgg tggctttcct ttcctgggaa ggcgggaatt ttgaagacgc
    35161 catcctcatt tctgaaagac tggtgcaaga ggataagttc acttcagtgc acattgaaaa
    35221 atatgaagtg gaagcgcgtg ataccaagct aggaccggag gaaattaccc gcgacattcc
    35281 caatgtgggt gaggatgcgg ttaaagacct ggatgaacag ggaatcgtcc gcatcggcgc
    35341 ggaggtaggc cctaacgata ttctagtcgg caagatcact cccaaaggtg aaaaagaatt
    35401 aactcccgaa gagcgcctgc tgcgcgccat tttcggagaa aaatctcgag atgtgaagga
    35461 cacctcgctg cgcatgcccc acggagagcg cggaaaagtt gtggatgtga aagtcttcac
    35521 ccgggaggat aatgtggacc tctccgctgg agtggatatg atggtgcgcg tctcggtcgc
    35581 gcagcgccgt aagatcaccg ccggcgacaa aatggcagga cgccatggta acaagggtgt
    35641 tgtttctcgc gttgttccgg tggaaaatat gccgtttctt aaagatggca cgccagtgga
    35701 tatcattctc aatccgctgg gggtgccggg ccgtatgaat atcggccagg tgctggaaac
    35761 gcacctgggt tgggcggcca aagaactggg ttttgaagcg attacgccag tatttgacgg
    35821 cgccaatgaa tacgaaattg aagctgaact cgccagagcc tggttgatgg attacacatg
    35881 gacagaagcg ggaaaaagag cctgggagtg gctgaacggc attgaccacg accctgaatc
    35941 gattaaagat gacgacgaag tgcggctgct gtacctggag cagaccctcg accacaagta
    36001 cgacatgggc cgcattgcca cggacccaac ctacgcccgc cagatcacgt tggaatcctg
    36061 gcttaatgaa agaggcttcg accttgcccc cattcttgat tctgctgacc ccaacacgga
    36121 agcagaaagg aaagaacgag acacccaggc gattaacacc tgcctccgtg tttggcttga
    36181 ggtaaatggc acagacccgt caaaaataaa agacgagaaa attcgagaag tggcagataa
    36241 agttgcgctt gaaacccggc agccggttcc cacgctcgga aagcagatat tacgcgacgg
    36301 caaaacaggc caggcttatg accagccggt taccgttggc gtaatgacca tgttgaaact
    36361 gcatcacctg gtagaagaca aggtgcatgc ccgttcaacc ggcccgtata gcctggtgac
    36421 acagcaacct cttggcggca aggcgcagtt tggcggacag cgtttcggtg aaatggaagt
    36481 ttgggcgctg gaagcatatg gcgcggctta tacgctgcaa gagatgctta cggtcaaatc
    36541 ggatgatgtt cagggtcgcg tgaacaccta cgagtcaatt gtcaaaggtg agcccataga
    36601 agatcccagc ctaccggctt ccttcagagt tttggtgaaa gagctgcaaa gcctgggttt
    36661 ggctgttgaa gcggtgatgg acaccaaaga aatcatccgc tttggaaaag atgaagaacg
    36721 tgtacgacct ccacgccttg aaaccgggtt actgggcctc ggcgaggagt tgcagggact
    36781 gctgtaacag ggcagtcgcg ggccgttttt tatggatttc tcttgaccat gaaaaaccct
    36841 cgtgttataa tttttcgccg ttgctctagt tacgtggtga tgtgtgcacc tctgccatca
    36901 gtgtgaaccg gtgaggccat cattgcagtc gatggtctca tcttaattag aaaagaacag
    36961 gcaatatcaa atttacggag gcaaagaaga agtgccaaca atcaatcagt tgatcagaaa
    37021 aggtcgtgag acgaaaaggg ccaaaagcaa ggcgcctgcg ttgcagtaca ctttaaactc
    37081 aatgaagcaa cgccgttccc gccttgaaaa aggcgctccg cagaagcgtg gcgtgtgcac
    37141 agtggtgagg acgatgaccc ccaagaaacc gaactcagcg ctgcgaaaga tcgcccgcgt
    37201 caggctttca aacgggatcg aggtcactgc ctatatccct ggcgaaggtc atcagctgca
    37261 ggaacactcg gtggtgctcg ttcgaggcgg tcgtgtaaaa gacctgcctg gtgtgcgcta
    37321 ccacattgtg cgcggcacgc tggacacctc tggggtcgcg aaacgttcac aggcgcgatc
    37381 gaaatacggt tcaaagaaac cgaaagcata accataggat ttgatggagt atttgcatgc
    37441 gaagatcaaa acccgaacca cgtgaagtca ccccggatgt gcgttataac agcgttcccg
    37501 tgacctattt catccaccgc atcatgaaac gcggcaaaaa gagtgttgcc accactatta
    37561 tgtatgatgc gctggaaatg attaaagaga aaaccggcaa gagccctgtg gaagtgctgg
    37621 accttgcgct taaaaacgtt ggtcctgtga tggaagtacg cccgcggcgt gtgggcggtg
    37681 ccacttacca ggtgcctatg gaggttcccg cgcaccgacg tatgacgctg gccatgcgct
    37741 ggattctgga tgccgcgacg agccgcaccg gcaattccta cgcggaaaag ctctcgactg
    37801 aactgatcga agcttcaaat aaccagggta acgcagtgcg caagcgcgaa gagactcata
    37861 aaatggctga ggctaaccgc gccttttccc attaccggat gtaaaccatt tctgatacca
    37921 ggaaacgatg tcacgcaatt ttccgttaga caagtgccga aatattggga ttattgctca
    37981 tatcgacgcg ggtaaaacca cgacgactga gcgtatcctc ttctattccg gcagaaccta
    38041 tcggctggga aacgtggatg aaggaaccac agttaccgat tggatggttc aagaacggga
    38101 gcgtgggatt acgatcgttt ctgccgcggt aaccgcggaa tggaagggat atcagatcaa
    38161 tgtgattgat actccgggac acatagactt taccgctgaa gtgcagcgct cgctgcgcgt
    38221 gttggatggc ggggtggttg tgtttgatgc tgttcagggc gttgagccgc agagcgaaac
    38281 cgtctggcgt caggctgacc gatacagtgt gccccgtatt tgtttcgtga ataaaatgga
    38341 ccgcgttggc gcgtccttgg aatacacttt gggcacgatc cgcgaaagac tgggcgcgca
    38401 gccaattcag atgcagctgc ctatcggcag cgaagccggt tttaaaggtg tggtcgatct
    38461 tttaacgatg caagcggtct attttgaaga tgaccttggc aaagagccca agtacgcccc
    38521 cattccagag gacctccgca agcaagccga agacctgcgt gagcaaatgg tggagcaaat
    38581 cgtagaatta aacgacggct taacggtgaa gtacctcgaa ggtgaaaaaa tctctatcga
    38641 tgagttaaaa caggctttgc gcgcggcagt cataaacacc agcgcgacac ctgtattttg
    38701 tggctcctca ttacgcaaca aaggcgttca gcttgttctg gacgccatcg tggattacct
    38761 gccgtctcct gtagatatcc cccccgttat cgcgcattcg cctgacggtg aaaaagaaat
    38821 ggagctgcca acagaagaca acgcgccatt gagcgcgctg gtgttcaaga ttgtcagcga
    38881 cccttatgtt ggccgcctgg cttacttcag ggtttattca ggcacgctca aaaccgggga
    38941 tacggtgttg aacccgatta aaggccgcag ggaacgcatc ggtcgattgc tgcgtatgta
    39001 cgccgaccgc cgcgaggaca tcaccgaggt taccgcggga gatatcggcg cagtgttggg
    39061 gctgaaagag tcctttacag gagacactct ctgcagcgtt aacaaaccct taatccttga
    39121 aagcattacc ttccctgaac cggtcatctc gattgcggtt gagccaaaat ctaccgcaga
    39181 ccaggataaa atggcgttgg cgctgcaacg tttagcggag gaagacccaa cctttaaggt
    39241 tcacacggac gaaagttcgg ggcaaacaat tattggcggc atgggcgaac tgcacctgga
    39301 tgtgctggtg gaccgtatgc tgcgcgaatt ccgcgtgcag gcaaacgtag gtaaaccgag
    39361 ggtttcttac cgagagacca tcacgcgaga ggtcaaggaa attgattaca aatatgtaaa
    39421 gcaaaccggt ggtcatggtc aatatgggca cgtggtattg aatttggcgc ccctggatcg
    39481 cggggctggt gtgaaatttg aaagcaaaat taccggtggc gttattccgc gtgaatatat
    39541 tcccgcagtt gaaaaaggcg tcaaagaggc tgtggagtca ggcgttttgg cggggtatcc
    39601 cgtgacagac ctgcaagtac agctggtgga tggttctttc catgaagttg actcgagcga
    39661 aatggcgttt aagatggcgg cgatattggg catgcgagaa ggcctgcaaa aaggcgcgtc
    39721 agtattgctt gaaccaatca tgcgaactga gatcgttgtg cctgaagaat atcttggcga
    39781 tgtgctcggt caggtgactt cgcgccgcgg ggttgtgctg gggatggacg tgcgcccggg
    39841 caatgcccag gcgattcatg cattattacc tttgtctgat atgttcgggt acgcaactga
    39901 gctgcgttct gccacgcagg ggcgtggcgt gttcacgatg gaatttgacc actacgcgcc
    39961 ggtgtcggct gctgtagcgg aaaaaatcat caattaatgc aatcattgga acttagtttc
    40021 taaggagagg aaacatggcg aagcagaagt ttgacaggag taagccgcat ttgaat
//
LOCUS       DAOTLW010000013        38357 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1280_length_38357_cov_10.451804, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000013 DAOTLW010000000
VERSION     DAOTLW010000013.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 38357)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38357)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38357
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1280_length_38357_cov_10.451804"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..885
                     /gene="pip"
                     /locus_tag="P5516_03500"
     CDS             <1..885
                     /gene="pip"
                     /locus_tag="P5516_03500"
                     /EC_number="3.4.11.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR01249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl aminopeptidase"
                     /protein_id="HRT91535.1"
                     /translation="NLHTLYYEEVGNPEGVAVVFLHGGPGVGAQPLYRRFFHPERFRV
                     INFSQRGAGKSSPSGELQQNDTWALVEDIEKLRQAVNVERWFVFGGSWGSTLALAYAQ
                     QYPERVRGLVLRGIFLGTRAEHNWLYVDGASRVHPESWQRFQSFIPPEERSDLPAAYY
                     QRLTSIQREVQMAAAEHWYHWEESLISLLPREAPRMSPDELLAFARIECHYIVNRLFL
                     PDDNYLLNNAHKIAHLPCWIAQGRYDIICPVVSAINLCQHLPEAELHIVPDAGHAISE
                     PGIVDSLMNGIEALAERG"
     gene            944..1390
                     /locus_tag="P5516_03505"
     CDS             944..1390
                     /locus_tag="P5516_03505"
                     /inference="COORDINATES: protein motif:HMM:NF012790.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HRT91536.1"
                     /translation="MATKTSKKKQQKQTKWLLPLILVSVAVIIGVVLSIAKEGHAAKG
                     LPSTVSVTEAAQRINEGAFLLDVRTAEEWNQAHVAGAVRIPLDELKSRLAEVPVDQDV
                     LIICRSGNRSGQARDLLRAAGLKRTTSISGGINAWMAKGLPVVSGP"
     gene            complement(1411..3273)
                     /gene="uvrC"
                     /locus_tag="P5516_03510"
     CDS             complement(1411..3273)
                     /gene="uvrC"
                     /locus_tag="P5516_03510"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HRT91537.1"
                     /translation="MVELTDHLKGILDTLPVKPGCYIMKNASGEVIYVGKAVNLRGRV
                     RSYFQEHADHERKVLKLVSQIADLEWIVVGSELEALILEMNLIKKHRPRFNVRLKDDK
                     RYPYIKVHLASDFPKVTVTRQMVNDGSRYFGPYTSVWAVHQTLDLLRRIFPYLTCDRE
                     ITGLDARPCLYYDIKLCSGPCIGAIDRAGYNQLISDLCAFLDGRTDPIVSRLQREMND
                     AAESLNFEKAALLRDQLQSIERVVEKQKVITSEQIDSDVLAIARADNEACVQVFFVRN
                     GRLMGREYFVMEGTEEIPDEQVMAEFIKQFYTEAAVIPDRVLLPNEVQEAQIIRQWLN
                     TRRGGNKVELRVPRRGTAHELMKMATENAVETLQALRAQWEADKNKQTQALSEIQQAL
                     NLPEPPNRIECYDISNTQGTTSVGSMVVFEQGTPSRAHYRQFNIKTVSGADDFASMEE
                     VLNRRFKRWQSAQEKQGLGAKPDPSFALLPDLLIVDGGKGQLSRAVKVLADYNLAGVV
                     PVVGLAKQQEELFQPGSGSSVVLPRHSQGFYLLQRIRDEAHRFAITAHRKARGRVGVA
                     SRLESIPGIGPARRRALLQQFGDIERIRQASLQELMSVPGINEELARAVQAGLE"
     gene            complement(3324..4391)
                     /gene="selD"
                     /locus_tag="P5516_03515"
     CDS             complement(3324..4391)
                     /gene="selD"
                     /locus_tag="P5516_03515"
                     /EC_number="2.7.9.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004756 - selenide, water dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0001887 - selenium compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(4320..4322),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HRT91538.1"
                     /translation="MDNPSLKPSGRQIRLTSLSNCAGUASKLSAEALAQVLRPMQELF
                     DPADTPELLAGLASPDDAAVWKLDEDRALVLTTDFFTPVVDDPFDYGRIAAANSLSDV
                     YAMGAAPFLALNIAAFPPDLDPAVSGEIFRGGATVAKQAGAVIAGGHSIQDKEPKYGM
                     VVAGMVDAQRMFSKGGAKPGDRLYLTKPLGFGTITTALKRQMVREEDLAEAVSWMVRL
                     NREASRIGMDCGVRAATDITGYSFIGHALELANASGVGLRVAFERLPVLPAAFTYASQ
                     FIFPGGAYDNRSYFQPFVEFSRKLPEDQTMLLFDPQTSGGLLLAVPAQHCADFEAAMQ
                     QGSAPCWQVGEVIKGSGIIFT"
     gene            complement(4569..5006)
                     /gene="dtd"
                     /locus_tag="P5516_03520"
     CDS             complement(4569..5006)
                     /gene="dtd"
                     /locus_tag="P5516_03520"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRT91539.1"
                     /translation="MRAVIQRVNYGRVVIEDRETANIGSGMVILLGVGPGDDEQKSAA
                     LAKKIAQLRIMEDAEGKMNLSLLDTGGAAVVVSQFTLFADTRKGNRPSFIGAAPPELA
                     SPLVDHFVAQLRSYGIPVQTGEFGAHMRVALENDGPVTIWMEM"
     gene            complement(5013..6017)
                     /gene="trpS"
                     /locus_tag="P5516_03525"
     CDS             complement(5013..6017)
                     /gene="trpS"
                     /locus_tag="P5516_03525"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HRT91540.1"
                     /translation="MTSKKGRVFSGARPTGRQHIGNYFGAIRNYVKLQDDYDCIYCIV
                     DLHALTTLDTTRDLRQNTLEMAWDWLAAGMDPKRSIIFVQSMVRQVTELHTILSMVTP
                     LGKLTDLPTFKEKVRENPKNINYGLVGYPVLMSADIALYRADVVPVGIDQAPHVEFAR
                     ETVRSFNFRYNTNALIEPKEKFTEFPKILGIDGVNKMSKSLNNDIELAATAEETTARV
                     MQMVTDPARQRRSDPGNPDICNVFSLHKMFTPPEKVAEINIECRRAGIGCVDCKRILA
                     ANMNAYFAPLREKRRVFENDPEGTWDMLRDGAARASLLAEETMVDVRAAVGLPALQKG
                     "
     gene            complement(6020..6262)
                     /locus_tag="P5516_03530"
     CDS             complement(6020..6262)
                     /locus_tag="P5516_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_255349558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(6230..6232),aa:Sec)
                     /transl_table=11
                     /product="SelT/SelW/SelH family (seleno)protein"
                     /protein_id="HRT91541.1"
                     /translation="MIISIEYCVVUHYTPRAVGLAAELLEAYETKIKSVTLIPSSGGA
                     FEVNVNGNLIYSKLQTGRHAEVGEVVALLRKKIEEG"
     gene            6261..6971
                     /locus_tag="P5516_03535"
     CDS             6261..6971
                     /locus_tag="P5516_03535"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRT91542.1"
                     /translation="MESAPEVKWLYYNVKSSVNARSTMDFGTIKPMTRFLKRLFDLLA
                     SALLLILLSPLLLILALLVRFKLGRPVLFSQERPGLRGHPFKLYKFRSMRDAYDDSGK
                     PLPDEQRLTPFGRFLRSSSLDELPELFNVLKGEMSLVGPRPLLTAYLERYTPDQARRH
                     EVLPGITGWAQVNGRNALSWEEKFELDVWYVDHQSLWLDIKILFITLWKALKREGINA
                     PGSATAPEFMGTGKPSFK"
     gene            complement(7036..7464)
                     /locus_tag="P5516_03540"
     CDS             complement(7036..7464)
                     /locus_tag="P5516_03540"
                     /inference="COORDINATES: protein motif:HMM:NF022896.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3021 domain-containing protein"
                     /protein_id="HRT91543.1"
                     /translation="MKKFFEHFLKGISTGCTIGVVVFLLIWLISGEETISMPTREFST
                     HVIGSMLVGLGFYLPSAIYENEKLAMWLRVLIHMGVGFIVFFIVASVAGWIPAAGGPG
                     AVILYVLLVFVISMLIWAGHYWHYACEAKKINAQLKKQQE"
     gene            complement(7475..7909)
                     /locus_tag="P5516_03545"
     CDS             complement(7475..7909)
                     /locus_tag="P5516_03545"
                     /inference="COORDINATES: protein motif:HMM:NF016296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LytTR family DNA-binding domain-containing
                     protein"
                     /protein_id="HRT91544.1"
                     /translation="MNVEIKISKDITEPYAVIYTNAITEEVQKIRSVLAFQSPVIPVS
                     DDEKIVVLEPDEIFMIRTENTEVIVYGKDTRFRSEKRLYELEDLLGNGFLRISKSTIV
                     NLKKIDYVEPSFNGMMAIRLKNGGKDYISRKYLPQLKNYLGI"
     gene            complement(8203..9108)
                     /locus_tag="P5516_03550"
     CDS             complement(8203..9108)
                     /locus_tag="P5516_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91545.1"
                     /translation="MDTPTAIPTAAPARMLLLDPFAAATAELNAYLAGFAEQHDLTLE
                     TQASPELPALGAETKVVLLLAELPGLDELTATSPETQFILIGNTALNPAGNLSVIQTR
                     EYDLTFMAGYLAQSIAWDWRTAGLIPSDTALSEPKTNAFINGARYLCGVCTPFYAPIV
                     SFPMLAQEPAQADAATWSGQVSVFGQHFVNTYFVDPAVATVETLDGLKGLEPAIFNDV
                     KLIGISTTPNPERFTALIGFDPLPALEQLLPQLIAGNGGLSLGAQVKITSFTDDTVIT
                     PAKIDNFNRVAADLATGMIVPLSIP"
     gene            9445..10665
                     /locus_tag="P5516_03555"
     CDS             9445..10665
                     /locus_tag="P5516_03555"
                     /inference="COORDINATES: protein motif:HMM:NF016013.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HRT91546.1"
                     /translation="MSDYSQEPGVVIPPSRIDESRRRRARKLLVPRGKSERALYLGEI
                     AQRLVPGVEFFLFSLVAGLVITLAILLDSPAIFILGALLAPFMAPVIGLGFSAVIGSF
                     TFFSRSIGAMFIGCALVFASGALGGWISRLFVDLPLSQSLRFTNFSIPDFLLLTIGIV
                     LAVYLTIRMPKQRTQVASVPLAYEIFIPVAVAGYGLTSRASGLFPNALIVAGVHIAWV
                     ILAGTLTLAVLKIRPFTFFGYLLTAAIFGSALYGLVISSALGVALQRQLAPFVTSTGV
                     PSAALIEPSASLSPADALAIQTPDPFGITATTAPTNTLMPTLTPTVTITPQPTPVWAK
                     VFSPTYSGVLVRQTPGFDGKYLTSLDNESLIQVLPEIEHVDGAYWAHVLLEDGREGWI
                     MRSLLLTATPAPNW"
     gene            10690..12150
                     /locus_tag="P5516_03560"
     CDS             10690..12150
                     /locus_tag="P5516_03560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HRT91547.1"
                     /translation="MTIHFGTDGWRAVISDTFTFTNLRLVAQAIADAVATPEWVNGNR
                     IGSEIDAHKMVVGFDTRFLSDRYAAEVARVLAANGFVVYLAQGDAPTPVISHAVRSLN
                     AIGGVMITASHNAPRYNGVKLKSASGGSALPEQCRRVEVYLNDNEALARGPNLMDMEQ
                     ARALKLVQRFNPVPAYYEHLRTLIKSDVIAENPQRFVVDSMYGSGRGIVRGFLQGTGC
                     EIAEIRGEMNPGFGGVHPEPIRQNLGALAGAIGAGMGSFGIATDGDADRIGAMDERSN
                     FVDPHKIMALSLRYLVEKRGLTGSVVRTVSTTRMIDRLAKKYGLTVHETPVGFNHIAD
                     YMLKEQVLIGGEESGGIGFSGHIPEGDGILMGLLIVEMLADAGTTLFDMVEDLQREVG
                     PVYYERIDQRLKYPVSKEKLVAKLVNEPPRSIGGEQLSEVSTMDGVKYIFRDDSWLLI
                     RPSGTEPVLRVYAEGRSPEMVKELLNFGSSIADGVS"
     gene            complement(12225..12797)
                     /locus_tag="P5516_03565"
     CDS             complement(12225..12797)
                     /locus_tag="P5516_03565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91548.1"
                     /translation="MALNNFDENPFDEEAFAEDLPVEPAKRSGRGGFLTVIVILGVVF
                     LLALLVLLFLAPKYLANQRAENMEQAALINAANTATAMAAVSMANQQQTALALTAQAL
                     LPTAAPTKTPVKAVVANTPVIAGMGLSDAELATVAALQTQMAGQGTVAPTELPDAGFA
                     DEFGLPLMGGMAVLLLVIIVFSRKLRLSSR"
     gene            complement(12788..13888)
                     /locus_tag="P5516_03570"
     CDS             complement(12788..13888)
                     /locus_tag="P5516_03570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91549.1"
                     /translation="MNAQLTQINPLTLIHRNRLLPVPGSVNVREGQRVAPEDIVAEAS
                     IPARHYLVDVVRALSAKTLSAAEKMITRKPGEVLQKNDIIAETGGLFSRVIRTPGEGR
                     VVSVNKGRVLIEAESTRIKMQAGLAGKVTQVIPERGATIQCYGALIQGVWGNALLGCG
                     PLTTDYESLSGELKTSAIGVTSRGAVLAGGTCSEAFLAAAAQEKIGGLILGSISSSLI
                     STALELPYALIILSGFGDHGMDELSRSLLATYIGRDVSINAVKWNRLTGDRPEVYIPL
                     EGEAHAYEEQAMYTSGQTVRLQTGAYAGRIGRIESLLPGVTLLPSGLRAAAARVVFGE
                     NLREIIPLSNLDVIFSNDRFIGAPDEGGVLWH"
     gene            complement(13926..15653)
                     /locus_tag="P5516_03575"
     CDS             complement(13926..15653)
                     /locus_tag="P5516_03575"
                     /inference="COORDINATES: protein motif:HMM:NF025309.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HRT91550.1"
                     /translation="MKSGLAQPMSVLGIEIGDVHTRGHLFDIVENSYRLIASSSAPTT
                     SFYPILDIGDAIYEVLSRIQEICGRTLIEKSGELILPALPTGDGVDQFYITTSCFSSL
                     QVVAVGLLNDVSLDSARKLCNATYANLLESIGINDRRSYSQQLDTLLTIRPDLVIFTG
                     GTDGGASRSLLRTAELVSTALQLLPRGTRPQILYCGNQVLSAQLKDVFERYTTVRSAP
                     NIRPSFEGENLEPALTELDALVMEKMNEEIGGLKRTSPLCTTPPQLKNQGFMRMIKFL
                     GRQYDPLKGVLGLDVGMNYSMAAYSNDRVSILNTFNFGIGSGLEAFLRKGDIKDISRW
                     LTFPVPDTVVSDALWNRSLFPLSVAATKEEIAIEQAVLRQMLQAMMIDLESREIMPSL
                     RFEPIVLSGCTVSHVADPVRSLLLALDGIQPLGISPLILDKHSLLPLLGTIAAHNSLL
                     AVQLLESTAFSNLATVVNITSKTRAGSPLLYARLDYADGNSFEVEVKQGSIISLPLAP
                     GATGLLRLRLTSRAEIEELALIREPIKVQGGVCGVVVDARGRPIVLPEDQGRRIELLK
                     EWEFLLGAA"
     gene            complement(15659..16330)
                     /locus_tag="P5516_03580"
     CDS             complement(15659..16330)
                     /locus_tag="P5516_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91551.1"
                     /translation="MSFNTEVIWVVISFILTLVVFSFLLGDIPFFRFVSAVLIGMTAG
                     YMTIVIIYQVLIARLAVPLLQGSRVALIPLLLSGMLLMKLSPRLSRLGNPSMALLVGV
                     GAAVAVGGAVLGTLFGQVKGALVFVDLARAQKGPTSLLLLVEGIFFLLGTLATLFYFH
                     FSARKVKGKEPQAHWSIRLLSSVGRVFIAITLGAVFAGVITAGLTALVERADFLLGTL
                     LFWVR"
     gene            complement(16330..18147)
                     /locus_tag="P5516_03585"
     CDS             complement(16330..18147)
                     /locus_tag="P5516_03585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91552.1"
                     /translation="MADIRCLICNRMNDASSPRCWYCRSPLPGYKEPPAPDEPGEGEI
                     PEPEPLAEISGRVEPTEESGESWEGEAPEWLARIRMLKEQDEQKTRETSGLDTEDSGL
                     PEWIINLRAAEPPVMAEKTAEEALLEDSDAKATTPADTYADSRFEETGGRASGEFTGE
                     PVEGETTSPAANKPFETAELRDGQPAHPIFEDDLPEWLSADAGMVETHSSQKDQTARP
                     FISKEENKLADGVLPAWLKAIRPLDAVSPATPDALYSPGEEGEGILAGISGTLRSQRL
                     EDREVKMPSRPHELVVTPMQQKNAELLYRLSNPDIEETSPVAITKKSRPQRKTLKIFA
                     AVILFLAVLLPYGFRSLPVIIPVLFPKEVVDTLGVIEGIPAGKPVLIAAQFEGGLAGE
                     LTLSSEPVFQHLVSHGVPLALMSTNASGYAILEEQLRRASAGVSGYLFSERVVNLGYL
                     PGGTIGLISLVGDIRAALPYSTALEPAWETTPIAGARTLADFAAILLLTDNPEIVRAW
                     VEQTGRIEKPPILLGVVSAQAAPLAQPYYDSGQVQGLVAGSSGALAYQMIRQVPGKAS
                     TTFASYQFALFIAALLIFLGGVVSLVTTTGAAAHKKGRG"
     gene            complement(18150..18767)
                     /locus_tag="P5516_03590"
     CDS             complement(18150..18767)
                     /locus_tag="P5516_03590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91553.1"
                     /translation="MKVLNYLIAAITGLIVLLGYFFPGTGISLARSPLLDWAVTLSGV
                     AGLVAILNLVFHVHWKRIQDKHPRRLYSGALIVTFLLTVVAGVFLDPGSAGFQKLVTR
                     VQVPVESTLMAVLAITLVFASLKLLQRQRNWMGFLFFLSVIIFLVLNSGVLAFSTEIP
                     ILTNLLSAFHRVPMAGARGMLLGIALGSLATGIRVLIGSEKPYNG"
     gene            complement(18764..19516)
                     /locus_tag="P5516_03595"
     CDS             complement(18764..19516)
                     /locus_tag="P5516_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91554.1"
                     /translation="MKAEPILGLLVVIFAAGWMIFLSYRKKSSEGRATFRRITAINRL
                     RSAVGLSVEDGSRVHISLGNGDLNEASNPSGLAGVDTLQRIGQLSSASDQPPVCTSGN
                     GGFTLLSKDVLRMVAGETQSTEMFDADHGRMAGATPFAYVLGTLEVMNEPAVRTNVFM
                     GSYGAEVGFLTTAAEENGAFTLAASDSIVAQSVFLATAREVMLGEELYAVPAYLVHRS
                     SHVASLKTQDLLRLIISGALLLGLLLKLAGIA"
     gene            complement(19513..19752)
                     /locus_tag="P5516_03600"
     CDS             complement(19513..19752)
                     /locus_tag="P5516_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91555.1"
                     /translation="MDPRFWSQWQDTFRRHGLTPLMLSLLEDGGIFRHFISQAMLAVA
                     PFFNHSAASWQAFADMLEDPAIALSFAEHLREEDA"
     gene            19904..20212
                     /locus_tag="P5516_03605"
     CDS             19904..20212
                     /locus_tag="P5516_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91556.1"
                     /translation="MPFFFTWFKPRPYSAANSREVHSLLEELIRIGIKEDYLSEIPGY
                     GYNSQCRHIRTREIGKRLHELGGNELMSWAFARVRKQAGKVPASHLEYAWNDIGGWQP
                     "
     gene            complement(20236..21375)
                     /locus_tag="P5516_03610"
     CDS             complement(20236..21375)
                     /locus_tag="P5516_03610"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT91557.1"
                     /translation="MKIACISTAQVPSTRAHSIQVMKVTQALAQNGEEVVLYLPGKEP
                     PAWQELADQYGLIEPFEIITLPTRPGLKRLDFSLAGVKSARRWGAQLVYTRMLWVALF
                     AGLRAMPFVLEMHDLPSGTFGPHIYRRILRMKAPKLVVYITSALKTLADQRFGVAARV
                     GEYLIAPDGVDLERYSDLPKPAAARKKLALEEAMTAGYSGGFYPGRGLELMQQLARAF
                     PQVRFLWIGGNDDQVEWWRARLKEADIHNVQLTGFIPNSQLPLYQAAADILVMPYSRA
                     FGGSGGGDISAVSSPMKLFEYMAAGRCILASDLPVLREVLNDQTAAFFEIENFSDLRE
                     KFAELLTDGTRREKLASAAKSAANAYSWKTRMTGIMRVMNNTLNH"
     gene            complement(21377..22729)
                     /locus_tag="P5516_03615"
     CDS             complement(21377..22729)
                     /locus_tag="P5516_03615"
                     /inference="COORDINATES: protein motif:HMM:NF024832.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRT91558.1"
                     /translation="MNSLFARIRKLLVEDHILTRVLKNTGYLFSSTSFGLLLSLVQSV
                     FAARLLGVAAFGLVGIITSFVSNVNRVFSFRMGEFIIRYLGKELTENNRQKAGAVVKV
                     AMLTEGFTSVVAFFVLLLLAPLGAKYIAKDMQALPMIQLFAVSILANMVSETSIGVLQ
                     ITNHFRTQAMINVIQSVVTALLILLAYLLHGGIIFVLSAYLVGKILLGISPAILAFYY
                     LPRHLQPGWWKTPLSLLPPFKELAHFTLSTNLSGTVKMIASESEPLWVGFFLDQQSVG
                     LYKLALSIVNPLMMPITPFVTTTYPEMTRSIVSRAWAQLRRLLRRVTLISASWTGLVL
                     LVMLIAGRWLIQLFYGAAFVPAFPATMVLLLGFGFANVFFWNRSLLLSFGQANIPLYV
                     LFSAALVKIALAFWVVPRWGIVGESALLSAYLIISTALLVFIGLRKARQGEKADLKGE
                     "
     gene            complement(22726..23703)
                     /locus_tag="P5516_03620"
     CDS             complement(22726..23703)
                     /locus_tag="P5516_03620"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT91559.1"
                     /translation="MARYLVTGAAGFIARRVIEQLIAQGHEVVGVDNLNDSYDPRLKE
                     WRLQQLARLPGFRFFRDDICRPEFYQSLSGEFTSFQGVIHLAARAGVRQAVEIPQVFL
                     QTNAAGTLNLLEWCRRSGTPKLVLASTSSIYGANPPLPTPENADSCHPLQIYAASKKA
                     AEVMAYTYHYLYGLDTTVVRFFTVYGPAGRPDMVMYRFTRWIVENEPVCVSGDGNQMR
                     GFTYLDDIADGVIKALKPLGYEIVNLGGHETITINELLQRIEHLVGKKANVHYIPGHP
                     ADVNANWADVTKARSLLGWEPKVLLAEGIPQLVNWYLEQRAWARDIPLP"
     gene            complement(23703..25019)
                     /locus_tag="P5516_03625"
     CDS             complement(23703..25019)
                     /locus_tag="P5516_03625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HRT91560.1"
                     /translation="MDYAQELIEKFKKKTAKVAVLGMGYVGLPFSTVFAQAGFAVTGI
                     DPVEQKMEMLNRGESYILDVPSLVVRQLVADGKLRGTTDYAILKEIDAVAICVPTPLR
                     HTGDPDLSFIISAAEGIAPYVHPGMVVVLESSTYPGTTRELVLPALTEKSGLKIGEEL
                     FLAFSPERVDPGREDWTTKNTPKVIGGMTEKCTEVSTAWYSMALDTVVPVSSTEVAEM
                     AKLLENTFRMINIGFVNELTLMCDRLGVDVWEVIDAAATKPFGFMRFNPGPGLGGHCI
                     PIDPMYLSWKMRSLNYTARFIELASEINTNMPRFVIGRVQDGLNEHAKPMKGSKVVVL
                     GVAYKPDIDDLRESPAIDVIRLLQAKGALVSYHDPYIPRVQEDEWQIESVTDLMLAVE
                     EADCVVIITNHKVYDYAAILNSAKLIIDTRNALGKLGVKSPKVIKL"
     gene            complement(25036..25686)
                     /locus_tag="P5516_03630"
     CDS             complement(25036..25686)
                     /locus_tag="P5516_03630"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRT91561.1"
                     /translation="MDTAEILREIAAQVATCQACTLYHSRKNAVPGEGSPTSEIMFIG
                     EGPGFHENEQGRPFVGQAGKFLDELLGEAGYTREQVFIANVVKCRPPNNRDPLPDELA
                     ACARFLDRQIETINPHVIVTLGRFSMAKFLPNARISEVHAKTYWVRGRLIVPMYHPAA
                     ALHQPSLKSTLINDFRTLPAAIASARAAAKRQPAQARTSAEGVKEIEEKPEQLSLF"
     gene            complement(25737..26246)
                     /gene="rimI"
                     /locus_tag="P5516_03635"
     CDS             complement(25737..26246)
                     /gene="rimI"
                     /locus_tag="P5516_03635"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: protein motif:HMM:TIGR01575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HRT91562.1"
                     /translation="MSETEVSGTLRIRRMQSEDLPQVFELDRSSFSLPWPERSFRFEL
                     ENNEVSRCWVAELLSESGAPLLVAIIVVWMIADEVHVATLAVSPAYRRQKIAQRLLAH
                     TLIDAYHSGANSSFLEVRRSNQAAITLYQRFGYREVAVRRNYYKDNQEDAILMDLAPL
                     NLENLKRFQ"
     gene            complement(26243..26911)
                     /gene="tsaB"
                     /locus_tag="P5516_03640"
     CDS             complement(26243..26911)
                     /gene="tsaB"
                     /locus_tag="P5516_03640"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HRT91563.1"
                     /translation="MLLAIDTSTQTSGIALYDEFVLIGEMIWRTASHHTVELAPAVQD
                     LMNRCMVSPAELKAVAVALGPGSFTSLRIGLALAKGLALSLNIPLLGIPTFEFQAAQQ
                     PLAAEPLLTVLPAGRSRLAVQEFSSSSGCWQPQDEARVMTAEEISDRISGPTRICGEM
                     SPSERQVIGRKWKNAMIAPPALAMRRPSMLAELAWKRWNEGEVDDPALLAPIYLHVAG
                     MIQE"
     gene            complement(26912..27406)
                     /gene="tsaE"
                     /locus_tag="P5516_03645"
     CDS             complement(26912..27406)
                     /gene="tsaE"
                     /locus_tag="P5516_03645"
                     /inference="COORDINATES: protein motif:HMM:TIGR00150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HRT91564.1"
                     /translation="MPILPAYSIDFVSRSPEQTRRLGMRMGTLLKSGDLLCLEGDLGS
                     GKTTFVQGLAHGWGSVDAVTSPTFVIVNQYRRAGGGLMHHLDAYRLTSIDEAIDLDLD
                     DMLSGGILVAEWAERIESVLPADRLVIKYAWLNEEQRNLLFIPVGERYKKMLDDFKRL
                     AFGG"
     gene            complement(27436..27732)
                     /locus_tag="P5516_03650"
     CDS             complement(27436..27732)
                     /locus_tag="P5516_03650"
                     /inference="COORDINATES: protein motif:HMM:NF017879.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HRT91565.1"
                     /translation="MGNFLLSPASCGEFENYNMGYNRSMLPELKLGDRVRLRKPHPCG
                     GYTWKIVRLGADIGLECDTCSHRVMLTRRELTRRMKTYLPIDAPQENPPSDVYQ"
     gene            27733..28383
                     /locus_tag="P5516_03655"
     CDS             27733..28383
                     /locus_tag="P5516_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HRT91566.1"
                     /translation="MLNLAPSLLFSRILTLIIALTIHEFSHALVADHFGDVTARNAGR
                     LTLNPLKHLDVFGSLMLLVVGFGWAKPVPVNPYALRRRSESALMWVSLAGPASNLLLA
                     ILAGLVLRFRMVPWRASAAGSMLPSPAEFLFTFLSINLLLMLFNLIPLSPLDGEKVLS
                     YLLPYRLQPGFESIQRYGPLLLMVLLFVLPLVGIDLIRWVMSPALTQLQKFIIGVG"
     gene            28383..29357
                     /locus_tag="P5516_03660"
     CDS             28383..29357
                     /locus_tag="P5516_03660"
                     /inference="COORDINATES: protein motif:HMM:NF014234.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HRT91567.1"
                     /translation="MSPQIALIGLNQVGASIGLALGAYKENITRVGHDADSARMRRLE
                     KEGVCDKTHTRLPDAVRGADMVILSLPTDLLRDALGLIAGEVFPQAVVVCISAVARVA
                     YQWARELLPPQQPFIVLQPVLHPARLRDWEENSSNPYADLFENTHMLVVTDSNTQEKA
                     FQLANDLCALLKAKPYFSEPLEADGITARIEQLPKLTAVALLSMLVEKPGWDDARKMT
                     NRAFFRFASLNTLFDEQEFFGVSNLVNRENITRALDELSATLAEIRQMIDAGDEEGLQ
                     KMYQTARQGYETWLTQRMSGEWDKVEKPPEALERGILTRLFGGRPVKK"
     gene            29367..29642
                     /locus_tag="P5516_03665"
     CDS             29367..29642
                     /locus_tag="P5516_03665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HRT91568.1"
                     /translation="MSFYCYIVECADGSYYTGWTLDPARRERQHNLGKGAAYTRVHRP
                     VRLVYIEEQPDRSTAMKRERAIKALNHTQKARLISKFTEMGDDRGDP"
     gene            29623..30762
                     /gene="galK"
                     /locus_tag="P5516_03670"
     CDS             29623..30762
                     /gene="galK"
                     /locus_tag="P5516_03670"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HRT91569.1"
                     /translation="MTVATPEGTVISPGRVNLLGEHVDYNDGVVLPAAIDRSVKIIFK
                     KNDSRLVSLKAVDLQDQCVIHLSHLEKKTSQEGKPLPDWALYPAGVMWSLQRHGLDLI
                     GFDAQFSSNIPIGAGLSSSAAIEVGFAVVCMSLCGWQLERLELAKLCQQAEREYVGVN
                     CGLMDQFACACGVEDHVVMFDTRSLASQTLPLPPATTLVIADSSLRRSLLTSAYNDRR
                     RDCESVVAYFNQLDPSIRSLRDLGEERLLAHEHALPPAAFRHARHVVEEISRVNQAID
                     LLKEKDARGFGRLMFSSHDSLRDLFEVSRPELDILVDIAAGFDGCLGSRLTGAGFGGS
                     TVSLVLDEKSDAFIHQLRDLYQKQTGLQAVVFSTRASRGAHLNPD"
     gene            30782..31777
                     /locus_tag="P5516_03675"
     CDS             30782..31777
                     /locus_tag="P5516_03675"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91570.1"
                     /translation="MLTSTNDRAIETHSLVKTFHTKHGFLTRTTRSTEAVRGITFHVN
                     KGELFGLLGPNGAGKTTTVKMLSTLLIPTSGSIHIFGQDILQHTRDIRKHIGFTFGGA
                     HGLYGRLTAIENLRYFAELYALPPRETHNRITELLELVGLRDRGNDRVETYSSGMQQR
                     LHLARVLLHDPELIFLDEPTVGIDPVGARELRSTIKLLKNQGKTILLTTHYMAEADEL
                     CDRLAIINHGQIIAMDSPSSLKSKLTSDAFVELKTSGENLPSVKNVLAQLDPDSEVTY
                     NQHAFVWEVNIRTRQPGVVMELLAPLLSSNLIQTLGVRNQTMEDVYVSIIQNGGA"
     gene            31774..32565
                     /locus_tag="P5516_03680"
     CDS             31774..32565
                     /locus_tag="P5516_03680"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91571.1"
                     /translation="MIGNFLRLGIRQMNVQLRMRVLSLWSLALFFTQPAIFSAIGMLL
                     ARAVGNTRPDLVYTVLGAGIMGMWSGLVFTSTYDIRADRREGTLELIVGSPTPLRRVV
                     GFRSLLNILAGSTSMLAAVAAALFIFDYSFSGINIPGVVISFSLLLLALWCMGIFLAN
                     ILAWSRLSGTFVDLLEMPIALLCGFMYPISVLPAWMQQISAVFPIRWGLETLQDALKG
                     SLIDSALVNKWLIALGVSFVYYLLARWFDRKVHDHIRITGELHSI"
     gene            32562..33362
                     /locus_tag="P5516_03685"
     CDS             32562..33362
                     /locus_tag="P5516_03685"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91572.1"
                     /translation="MIKRLGHLFRLYFFQAWFSYRSLFAWSTPFSYATSKFGFPFFAM
                     LFFVFMGKFVGFLDPMFIVIGNILMMPTTNGLYGISMTIGNERQFGAMSYLLGSPAPR
                     APLFLGRAFFHILDGFVTVLVALPIAMLIFHIDFAEVNLFMLACCLVLLSITTTGLGF
                     IMGTVSLLSRDGWMFTNTLGQLLYILIGVNIPIERLPGFLQPLSGYLPMTRGILATRL
                     ALGGAGWSEVASLLAGEILVAAVYMVFGYTLFRLVERASLSTGSLDSI"
     gene            complement(33391..33942)
                     /locus_tag="P5516_03690"
     CDS             complement(33391..33942)
                     /locus_tag="P5516_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HRT91573.1"
                     /translation="MKKRETYPIEIAGVHRDLTLFEIKPGLKIAILNILGDMELVQSC
                     AAALAEKLTAVDYDLLLTAEAKSIPIAHALAAETMKPYVVLRKVYKPYMGDALKAETL
                     SITTGEPQYLYLDEKDRELMMGKKVVIVDDVISTGSTLQGMRLLVQKAGAEVVAEAAI
                     LTEGERARWHEIISLGHLPVFTD"
     gene            34431..34772
                     /locus_tag="P5516_03695"
     CDS             34431..34772
                     /locus_tag="P5516_03695"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HRT91574.1"
                     /translation="MGEYFAGQRKAFDLPLDWQSIRGFQREALLELLKTPYGEVRTYG
                     EISRALGKPAAARAVGMALAKNPLPILIPCHRVVAASGHLTGFSAGDGLNSKAWLLQL
                     EGHRVVTEKLA"
     gene            34750..35661
                     /locus_tag="P5516_03700"
     CDS             34750..35661
                     /locus_tag="P5516_03700"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRT91575.1"
                     /translation="MSLKSWLKFILLGIAWGSSFFWIKIGLQEVGPFTVVFFRIFFAL
                     IGLTVFFLLNRRKPLLKAWRLYLFLGVFYVALPFALVAWSEKYITSGLASILNSMQPL
                     ATSLIAAVFIQEERLNARRIVGLLLGFGGVLVLMSDKLSGEAINQIYGIIAMVAAVFC
                     YGGSSVFARMHNQGVKPEDQAIGQFVFGILFIFPTMLAVESPFIMPVRPVSYLAFAWL
                     GLIGSFFAAIAWYSLLDEIGPSRVSMTTYLFPLVGVLLGAILLHEAVDWRVIVGGLMI
                     LGGIVIVNSGKRNNQTPAQVQADPFEG"
     gene            35663..36577
                     /gene="era"
                     /locus_tag="P5516_03705"
     CDS             35663..36577
                     /gene="era"
                     /locus_tag="P5516_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HRT91576.1"
                     /translation="MMEKQEFRAGYVAVVGRPNVGKSTLINALLGQKIAAVSPKPQTT
                     RRNQLGILSLPEAQLIFVDTPGLHDPQHKLGDFMNQAAGEALRDADILLWLLDASAPF
                     TSEDHLIAEKLGGMRSLPPVWMGLTKTDLVNEKTLEERKRQAVELYPAQDVLTLSVKN
                     KASLDALLEVMISRLPLGEAFYESDTVTDLYERDIAAELIRESVLTFVQEEVPHCVAV
                     KIDAYEERPGEKAYIEATLFVERDSQKGILIGKGGEMIKKIGTHSRQEIEEMSGCKVF
                     LELKVRVNKNWRNSPDALELLGYKQVAG"
     gene            36581..37252
                     /locus_tag="P5516_03710"
     CDS             36581..37252
                     /locus_tag="P5516_03710"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRT91577.1"
                     /translation="MLTWAFWLAGFFALLDWAAVWKGWRKRLYLAKPAVMVFLIFWTL
                     QLTGWRNEMTWFGAGLVFSLIGDIALMLNPRFFMVGGTSFLFAQVAYFIGFNTSPAPL
                     SGGVFLVAVLVGAAAAVIFKRIRPGILRVPRGRRFLAALCLYGLTLTLMLLSALLTLF
                     RPEWQYQPAILAAIGALLFFSSDTMLVYDRFVRRFNHAQSYVHLTYHLAQFGIISGAV
                     LHCLR"
     gene            complement(37258..38175)
                     /locus_tag="P5516_03715"
     CDS             complement(37258..38175)
                     /locus_tag="P5516_03715"
                     /inference="COORDINATES: protein motif:HMM:NF014627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HRT91578.1"
                     /translation="MTAQKGTVTALWPRFTSAIGPLEFTWKTAREYLVVLLGALIQAL
                     AMRLFLVPGQLVSGGISGAAQIINHFAPIPIGLMVFIGNVPLFFLGWRFLGGPRFALR
                     TAIAVGAFSLFTDLLVFIIPAQGVTRDNVLNAVFGAVLLGVGLGLVYRGGGTSGGSDI
                     LGRILNHRLGISISTAYLITDSIVVLAGGFAFSWEKALYGMVVIYISGLAAEMISEGS
                     GIFRTAIIISPYHEKISSEIMSVLSRGVTVLSGKGAYTGTDKPVLYCVITRAEVNQLK
                     QVVKEIDPQAFMVIGVTHEALGEGFRPLK"
     gene            38282..38353
                     /locus_tag="P5516_03720"
     tRNA            38282..38353
                     /locus_tag="P5516_03720"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:38314..38316,aa:Met,seq:cat)
BASE COUNT         8697 a        10797 c        10210 g         8653 t
ORIGIN      
        1 aacctgcaca cgctttatta tgaagaagta gggaaccctg aaggcgttgc ggtggtattt
       61 ttacatggcg gtcctggcgt gggtgcacag ccactctatc ggcgtttctt tcatcctgaa
      121 cgtttcaggg tcatcaactt cagccagcgg ggtgctggca agagcagccc gtcaggcgaa
      181 ttacaacaga acgatacatg ggcattagtg gaagacatcg aaaaactgcg tcaagcggtg
      241 aacgttgaac gctggttcgt ttttggcggc tcgtggggca gcacactggc tttagcctac
      301 gcgcagcagt atccggagcg cgtgcgtggc ctggtgttac gcggtatctt tttaggtacg
      361 cgcgctgaac acaactggct gtatgttgat ggcgcttcac gcgttcaccc tgagagctgg
      421 cagcgtttcc aatcgttcat ccctcccgaa gagcgctctg atctaccagc ggcgtattac
      481 caacgcctca cctcaatcca gcgcgaggtg cagatggcag ccgccgaaca ctggtaccac
      541 tgggaagaaa gcctcatcag cctgctgccg cgtgaagcgc cgcgcatgtc gcctgatgaa
      601 ctgctggctt tcgcgcgcat cgagtgccat tacatagtga acaggctttt cctgcctgat
      661 gataactact tgctgaacaa cgcgcataag atcgctcatc tcccctgctg gatcgcccag
      721 ggtcgttatg acatcatctg cccggtggtt tcggcgatta acctctgcca gcatctgcct
      781 gaggctgaac tgcacatcgt gccagacgct ggtcacgcca tttctgaacc ggggattgtg
      841 gacagcctga tgaacggcat cgaagccctg gcagaacgcg gttgacctct gtcacttatc
      901 atgaaatcca acatctaatc tgaaaactta attgaggtgg agcgtggcaa ccaaaacgtc
      961 gaagaaaaaa caacaaaaac aaaccaaatg gctgctccca ttgatcctgg tcagcgtggc
     1021 agtgatcatt ggcgtagtgt tatccatcgc caaagaaggt cacgcagcca aaggtctccc
     1081 gtcaactgta tctgtaaccg aagctgctca aagaataaac gaaggcgcgt tcttactgga
     1141 tgtacgcaca gcagaagaat ggaaccaggc gcatgtggca ggagcagtgc gcatcccgct
     1201 ggatgagctc aaatctcgcc tggcagaagt gccggttgat caggacgtgc tcatcatctg
     1261 ccgcagcggt aatcgcagcg ggcaagcgcg tgacctgctg cgcgcagcgg ggttgaagcg
     1321 taccaccagc atcagcggcg gcatcaacgc ctggatggcg aagggcctgc cggtggtttc
     1381 cggcccctaa tttacgcatt cttcaattat tcactcaagc cccgcttgca ccgcccgggc
     1441 gagttcttcg ttaatccccg gtactgacat cagctcttgc aatgaagcct ggcggatgcg
     1501 ttcaatatca ccaaactgct gcaacagggc gcgccgccgc gccggtccaa ttcccggtat
     1561 gctctcaagg cgggacgcca cacccaccct gccacgggct ttgcggtgcg cggtaatggc
     1621 aaaacggtgc gcctcatcac ggatgcgctg caacaggtaa aaaccctgag aatgccgcgg
     1681 taataccaca gaactgccgc tgccaggctg gaataattcc tcctgctgct tggccaaccc
     1741 caccactggc accacccccg ccaggttata gtcagccaga accttcaccg ccctgcttaa
     1801 ttgacctttg ccgccgtcca cgataagcag gtcaggcagc aaagcgaacg agggatcggg
     1861 ctttgcgccc agcccctgtt tctcttgcgc agattgccag cgtttgaaac gccggttgag
     1921 cacctcttcc atactggcga agtcatccgc accgctgacc gtcttgatat tgaactgacg
     1981 gtaatgcgcg cggctggggg taccctgctc gaacaccacc atgctgccca ccgaggttgt
     2041 gccctgcgtg ttggaaatat cgtagcactc gatgcggttg ggcggctcgg gcaggttgag
     2101 cgcctgctgg atctcgctga gtgcctgggt ttgcttgttc ttgtccgcct cccactgcgc
     2161 gcgcagcgcc tgcaaagttt caacggcatt ttcggtagcc attttcatca actcatgcgc
     2221 ggtgccgcgg cgcggcacac gtaactccac tttattgcca ccacggcggg tgttcaacca
     2281 ctggcggatg atctgcgcct cctgcacctc gttgggcaac aacacgcggt ctgggatgac
     2341 cgcagcttcg gtatagaact gtttgatgaa ctccgccatc acctgttcgt cgggaatttc
     2401 ctcagtgcct tccatgacaa aatactcacg tcccatcagg cgaccattgc gaacgaaaaa
     2461 cacctgcacg caagcctcgt tatccgcgcg cgcaatcgcc agcacgtctg aatcgatctg
     2521 ttcgctggtg atgacttttt gtttttccac cacccgctcg atcgactgca gttggtctcg
     2581 cagtaaagcg gctttttcaa aattcaaact ttccgccgca tcgttcatct cgcgttgcag
     2641 ccgcgaaacg atagggtctg ttcgcccatc caaaaaggcg cacaggtctg aaataagctg
     2701 gttgtacccg gcgcgatcga tcgcgccgat gcaaggtccg ctgcataatt tgatgtcgta
     2761 atacaggcag ggtcgcgcgt caagcccggt gatctcacga tcgcaggtga gataggggaa
     2821 gatgcgccgc agcaggtcaa gcgtttggtg caccgcccac acgctggtat aggggccaaa
     2881 ataacgagag ccatcattga ccatctgacg ggtcacggta accttgggaa aatcgctcgc
     2941 caggtgcacc ttgatataag gatagcgctt gtcatccttg agacgcacat tgaaacgcgg
     3001 gcggtgcttc ttgatcaggt tcatctcgag gataagcgct tcaagttctg agcccaccac
     3061 aatccactca aggtcggcga tttgcgaaac taacttgagc accttgcgtt cgtggtcagc
     3121 gtgttcctgg aagtaggagc gcacacggcc gcgcaggttg accgccttcc ccacgtaaat
     3181 gacctcgccg gaggcgttct tcataatata acaaccaggt ttcaccggca gggtatccag
     3241 aatacctttg aggtgatcag tcagttcaac cataatggga ttttagcaca gggcggggac
     3301 tcagcgagga gcggtcggtt gcgtcaggta aaaatgatcc cgctgccttt gatcacttct
     3361 cctacctgcc agcagggagc gctaccctgc tgcattgcgg cttcaaaatc cgcgcagtgt
     3421 tgggcaggaa cggccagcag cagcccgccg ctggtttgag ggtcaaaaag cagcatggtc
     3481 tggtcttctg gcagcttccg actgaattct acaaacggct gaaaatacga acggttgtca
     3541 tacgctccgc ccgggaagat gaattgagat gcgtatgtga aggcggctgg cagcacaggc
     3601 aggcgctcaa acgccacccg cagccccaca ccactggcgt tggcgagttc cagcgcgtga
     3661 ccgatgaaag aataaccggt gatatccgtc gccgcgcgca cgccgcaatc catacctatt
     3721 cggctggctt cgcggttgag ccgcaccatc cagctcactg cttctgccag gtcttcctcg
     3781 cgcaccatct ggcgcttgag cgcggtggtg attgtgccaa agcccaacgg cttggtcagg
     3841 taaaggcgat cgcctggctt ggcgccgccc ttgctgaaca tcctttgcgc gtcgaccata
     3901 cccgctacca ccatgccata tttgggttct ttatcctgga tggagtgtcc ccccgcaatc
     3961 actgcccccg cctgcttggc caccgtcgcc ccgccgcgaa aaatttcacc gctgacggca
     4021 gggtcaaggt caggtgggaa agcggcgatg ttcagcgcca ggaaaggcgc agcccccatg
     4081 gcgtagacat ctgagaggct gttggcagca gcgatgcgcc cgtaatcaaa cggatcatcg
     4141 accaccggcg tgaagaagtc ggtggtcaac accagggcgc ggtcttcatc caatttccac
     4201 accgccgcat catcaggcga agccaacccg gcgagcagct caggggtgtc ggcagggtca
     4261 aaaagctcct gcatggggcg cagcacctgc gccagcgctt ccgcgctcag cttggatgct
     4321 caacctgcgc agttcgagag gctggtaaga cgaatttgcc tgcccgaggg tttgagagaa
     4381 ggattatcca tggagtaatt atactggaga tgaaagttga caggcgccgc aggcggctac
     4441 aggcgtaaac agcgcaccca tcgccgtgtt caacaatgag gatctacagc gctgctcttc
     4501 atgccaggcc agagacgaaa aaccatcact tataaaaggg gtgagttacg aacaggcatt
     4561 cagcgcgttt acatttccat ccagatggtc accgggccat cgttttccaa cgccacgcgc
     4621 atgtgcgcgc cgaactcacc ggtctgcact ggaatgccat aagaacgtag ttgggcaaca
     4681 aaatgatcga ccagcgggct ggcgagttca ggtggggcag cgccaataaa cgaggggcga
     4741 ttacctttgc gggtatcagc aaaaagcgtg aactgtgaaa ccaccacggc tgccccaccg
     4801 gtatccagca gcgagaggtt cattttacct tccgcatctt ccatgatgcg cagctgggcg
     4861 atcttcttcg ccaacgcggc tgacttttgc tcgtcatcac ccgggcctac gcccagcaga
     4921 atcaccatgc cgctgccaat attcgcggtt tcgcggtctt caattaccac ccggccgtaa
     4981 ttgacccgtt ggatgacagc gcgcatcgcc ggttatcctt tctgcagcgc tggcagaccc
     5041 accgccgcgc gcacatccac catggtctct tcagccagca ggctggcgcg cgccgcgccg
     5101 tcgcgcagca tgtcccaggt gccctcgggg tcattttcga agacacggcg tttttcacgc
     5161 aggggggcga agtaagcgtt catgttggct gccagaattc ttttacaatc cacacagcca
     5221 atgcccgcgc gacggcactc gatatttatt tctgctacct tctcaggcgg ggtgaacatc
     5281 ttatgcagcg aaaaaacatt gcaaatatcg gggttgccgg ggtcgctgcg acgctggcgc
     5341 gccggatcgg taaccatctg catcacacgc gcggtagtct cttccgcagt ggcagcaagc
     5401 tcgatatcgt tgttgaggct cttgctcatc ttgttcacac cgtcaatacc cagtatcttg
     5461 ggaaattcgg tgaatttctc tttgggttcg atcagcgcgt tggtgttgta acggaagtta
     5521 aacgagcgca ccgtttcccg cgcaaactcg acgtgcggcg cctgatcaat tcccaccggc
     5581 accacatcgg cgcggtaaag ggcgatatcc gcgctcatca gcaccgggta gcccaccagc
     5641 ccataattga tattcttcgg gttttcgcgc accttttctt tgaacgtggg cagatcggta
     5701 agttttccca agggcgtgac cattgaaagg atggtgtgca gttccgtcac ctggcgcacc
     5761 atcgattgta cgaagatgat ggaacgtttc ggatccatgc ccgctgccag ccaatcccag
     5821 gccatttcga gggtgttctg ccggagatcg cgggtggtat ccagcgtggt cagagcgtgc
     5881 aggtccacaa tgcaataaat gcaatcgtaa tcgtcctgta gcttaacgta attacggatg
     5941 gcgccaaagt agttgccaat atgctggcgc ccggttggcc tggcgcccga aaaaacccgt
     6001 ccttttttgc tggtcatggt tacccttcct cgatcttttt tcttaataaa gccacaacct
     6061 cgcccacttc agcgtggcga ccggtttgca atttggagta aatgagatta ccgttcacgt
     6121 tcacttcgaa agcgccgccg ctggacggaa taagggtgac cgatttgatt ttggtttcgt
     6181 aagcttccaa cagttcagcc gccagaccga cggcgcgcgg ggtgtagtgt cagacaacgc
     6241 agtattcaat ggagatgatc atggaaagcg ctcctgaggt taagtggctc tattataacg
     6301 taaagtcatc agtgaacgcg cgttcgacaa tggattttgg tacaatcaaa ccaatgacgc
     6361 gttttctcaa acgtctcttc gatttactcg caagcgcctt gctgcttatc ttgctctcgc
     6421 cgctgctgct gatactggcg ttgctggtgc ggttcaagct gggcagacca gtgctcttca
     6481 gccaggagcg accagggctg cgcgggcacc ccttcaagtt gtacaagttc cgcagcatgc
     6541 gcgacgctta tgatgattca ggcaaaccgc tgcccgatga acaacgcctc acccctttcg
     6601 ggcgtttttt acgctcctcc agtctggatg aactgcccga gctcttcaac gtgctcaaag
     6661 gcgagatgag cctggtcggc cctcggccgc tgctcaccgc ctacctggag cgctacacgc
     6721 ccgaccaggc gcggcgccac gaagtgctgc caggcatcac cggctgggcg caggtgaacg
     6781 ggcgcaacgc gctcagctgg gaagagaagt tcgagctgga tgtgtggtat gtagaccacc
     6841 agtcattgtg gctggatatc aagattctct tcatcaccct gtggaaagcc ctcaaacgcg
     6901 agggcatcaa cgccccagga agcgccaccg cgccagagtt catgggaaca ggcaaaccct
     6961 cgttcaaata aacacccccc ttacgcgggg ggtgtgtgct acaagacagg cttcttgcca
     7021 gttagagcag aacggctatt cttgctgctt cttcaattgc gcgttgatct tcttggcttc
     7081 gcaagcgtaa tgccagtaat ggccggccca aatcagcatc gaaataacga acaccagcaa
     7141 aacatacaga atgaccgcgc caggcccgcc tgccgccggg atccagccag caacagatgc
     7201 aacgatgaaa aatacgatga acccgacgcc catatgaatc agcactcgca gccacatggc
     7261 cagcttttca ttttcataaa tggcgctcgg caggtagaaa cccagtccta ccagcatgct
     7321 gccaatcacg tgggttgaga attctcgtgt gggcattgaa atggtctcct cgccgctgat
     7381 caaccagatc aacaagaaaa ccaccacacc aatcgtgcac ccggtcgaga tcccttttag
     7441 aaaatgttcg aaaaattttt tcatcgcaaa cctcctatat tcctaaatag tttttaagtt
     7501 gtggtagata ttttcttgaa atgtaatctt tccctccgtt ttttaatcga atggccatca
     7561 tgccattgaa ggacggttcc acataatcaa tcttcttcag gttgacaatc gtggatttgg
     7621 agatgcgcaa gaaaccgttc ccgagaaggt cctctaactc gtacagccgt ttttccgacc
     7681 ggaacctggt atctttgccg taaacgataa cttccgtgtt ctctgtgcgg atcatgaaaa
     7741 tttcatcggg ttccaataca acgattttct cgtcatcgct aacaggtatg accggtgatt
     7801 ggaacgctaa aacgctgcga atcttctgta cttcctcagt gatcgcgttg gtgtagatga
     7861 ccgcgtatgg ttcagtgatg tctttggaaa tttttatctc aacgttcaca gctgctgcct
     7921 ccttgaatga cttatttcac catcagtata acggtttatc tttcgctctt gccgtgtttt
     7981 cgggtaggtg gtcgtttctc gcttgcaacc tgcctgattc tgcctgtcaa atacaagcac
     8041 tcaacaggta aaaacaaccc gcaaggctat tcactgtggt aattgaccat cagcagcctc
     8101 gtgggagtta tcaactacct caaccaggca aggcgagcct gatggacctg tcgcaaaaga
     8161 gcggcatcct gcgtttggcc gctgcagaac gttggaagcg tattagggga tgctgagggg
     8221 gacaatcatt ccggtagcca ggtcagcggc cacgcggttg aagttgtcaa ttttcgccgg
     8281 ggtgatgact gtatcatcgg tgaaggaagt gatcttcacc tgcgctccca ggctgaggcc
     8341 gccattaccg gcgatcagct gaggaagcaa ttgttcaagc gcaggaagag ggtcaaagcc
     8401 gatcaaggcc gtaaaacgct cggggttggg ggtagtggag attcctatca gtttcacatc
     8461 gttaaaaatg gccggttcca gacctttaag cccgtccagc gtttcaacgg tggcaacagc
     8521 ggggtcaaca aagtaagtat tcacaaagtg ctgcccgaac acgctcacct gtcccgacca
     8581 ggtcgcggcg tctgcctgcg cgggttcctg ggccagcata gggaaagaca caattggggc
     8641 gtagaaggga gtgcataccc cgcacagata acgcgcaccg ttaatgaagg cgttcgtttt
     8701 tggctcgctt aaggccgtgt cgctgggaat caggccggca gtgcgccagt cccaggcaat
     8761 gctttgggcg aggtatcccg ccataaaggt cagatcatac tccctggtct ggatgacgga
     8821 aaggtttcca gccgggttca acgccgtatt gccaataagg atgaactgcg tttcaggtga
     8881 ggtcgcggtc agctcgtcca gtcccgggag ttctgccaat agaaggacaa ctttggtttc
     8941 agcaccaagc gcgggcagtt caggtgaggc ctgggtttcc aacgtgaggt catgctgctc
     9001 agcgaacccg gcgaggtatg cgtttaactc agctgtagcc gcggcaaacg gatccagcag
     9061 cagcatacgc gcgggagcag ccgtggggat ggcggtcggt gtatccactg gctcctgtgt
     9121 aagcggtgtt gcgctgggta ctggcggctg gtcagtcggt ttcgagcagc ctgtcatcag
     9181 cagtccaatg atgaaaagca gggataagat tcgcttaagg tgaagtctat tgtggcgatc
     9241 cataggggta ttttacctta gttactgcca atattgagtg tattataata atgtcatgat
     9301 ttcttgcgac caaactggcc ataacgccag agagctccgc caccataagg gcaaaccgag
     9361 taccacctcc gcgccggcag cacttttttt gcgcgcggcg ggattttttt tccacttgtt
     9421 tagattggaa acagcgcgat aacaatgagc gattactcac aggaaccagg tgttgtaatt
     9481 ccgccaagcc gtatcgatga gagccgccgg cggcgcgcgc gtaaattact ggtcccacgg
     9541 ggaaaaagtg aacgcgccct ttacctcggc gaaattgccc agcgcctggt gcctggggtt
     9601 gaattctttt tgttttctct ggtggctggt ctggttatca ccctggccat cctgctcgac
     9661 agtcccgcga tcttcatcct tggggcttta ctggcgccgt tcatggcgcc ggtcattggg
     9721 ctgggttttt cagccgtcat cggctccttc accttttttt ctcgctccat cggggctatg
     9781 ttcatcgggt gcgcgctggt cttcgccagc ggtgcgctgg ggggctggat ctcaaggctg
     9841 ttcgtagacc tgccactttc acagtcgctc cgttttacca atttcagtat tcccgatttt
     9901 cttttgttga ccattggcat tgtgctggct gtttacctga ccatacgtat gcctaaacag
     9961 cgcacacagg tggcgagcgt cccactggct tatgaaatct tcatcccggt ggcggtagcc
    10021 ggctatgggt taacctccag ggcaagcggt ttgttcccta acgccctgat agtggctggc
    10081 gtgcacatcg cctgggtgat ccttgccggc accctgaccc tggcagtgct caagatacgc
    10141 ccgttcacct tcttcgggta cctgctaacc gcagccattt tcggcagtgc gttgtatggg
    10201 ttggtcattt ccagcgcgtt gggtgttgcc ctgcagcgtc aactggcgcc atttgtgacc
    10261 tccacaggcg tcccctccgc agcgttgatt gaaccatcag ccagcctgtc tccggcagac
    10321 gcgctggcaa tccagacgcc cgatccgttc ggcatcaccg ccacaacagc ccctaccaac
    10381 acgctgatgc ccacgcttac cccgacagtc accatcaccc ctcaacccac accggtgtgg
    10441 gcaaaggtgt tcagccctac gtattctggt gtgttggtca ggcaaacccc aggctttgat
    10501 ggaaaatacc tcaccagcct ggataacgag tcgctgatcc aggtactccc cgaaattgaa
    10561 catgtggatg gcgcttactg ggcgcacgtt ctgcttgaag atggacggga aggctggatt
    10621 atgcgcagcc tgttacttac cgccacacca gcacccaact ggtaaaatac atttcactgg
    10681 agaaaaccaa tgacgattca ttttggcact gatggctggc gagctgtaat ctcggatact
    10741 tttaccttta ccaatttacg cctggtggcg caggcaatcg ccgacgcggt tgctacccct
    10801 gaatgggtca acggcaatcg gataggctcc gaaatcgatg cgcataaaat ggtggttggg
    10861 ttcgacacgc gtttcctttc ggaccgctac gctgccgaag tggcgcgcgt actggcagcc
    10921 aacggctttg tcgtgtacct ggcgcaggga gacgcgccaa ccccggttat ttcgcacgct
    10981 gtgcgcagtt taaacgccat cggcggcgtc atgatcacag cctctcataa cgccccgcgt
    11041 tataacgggg tcaagctaaa atccgcgtcg gggggttcag cgctgccgga acaatgtcgc
    11101 cgggtagaag tttacctgaa cgataacgaa gccctggcac gcgggccgaa cttgatggat
    11161 atggaacagg cgcgcgccct taagttggtc caacgcttca atcccgtccc ggcttattac
    11221 gaacatttac gcacgctcat taaaagtgat gtgattgccg agaacccgca gcgctttgtc
    11281 gttgattcta tgtacggctc aggacggggt atcgtgcgtg ggtttctaca gggcactggc
    11341 tgtgaaatcg ctgagatccg cggagaaatg aaccccggct tcggtggggt tcacccggag
    11401 cccatccgcc agaacctcgg cgcgctggct ggcgcgattg gggcagggat gggtagtttt
    11461 ggcatcgcca ccgacggcga tgctgaccgc atcggcgcga tggacgaacg cagcaatttc
    11521 gttgacccgc acaaaatcat ggccctgtca ctgcgctacc tggttgagaa acgtggcctg
    11581 accggttctg tggtgcgcac ggtttccacc acacgtatga tcgaccgcct ggccaagaag
    11641 tacgggctca cagtgcacga aaccccggtc ggttttaacc acatcgccga ttatatgctg
    11701 aaggaacagg tgttgattgg tggggaggag tcaggcggaa taggcttcag cggtcacatc
    11761 ccggagggcg acggtatttt aatgggctta ttaatcgtgg aaatgctcgc cgacgctgga
    11821 acaaccctct tcgacatggt ggaggatctg cagcgcgagg tgggcccggt gtattatgaa
    11881 cgtattgacc aacgcctcaa gtacccggtt tcgaaagaaa aattggtagc gaaactggtc
    11941 aacgaaccac ccagatcaat cggcggcgag cagctgagcg aagtgagcac catggatggc
    12001 gttaagtata ttttccgcga cgattcctgg ctgctcatcc gtccctcagg aactgaaccg
    12061 gtactgcgtg tgtatgcgga gggaaggtcc cccgagatgg ttaaggaatt gctcaacttt
    12121 ggcagctcca tcgcggatgg cgtttcttga tttcctggtc aatgagaaat gaaaaatccg
    12181 gggctgaagg tgatccgccc cggatcatgt tttaatcggt aagtttacct ggaggacaaa
    12241 cgcaatttac gactgaaaac gatgatgacc agcagcagca cagccatccc acccatcaag
    12301 ggcagaccaa actcatcagc aaaacccgcg tctggcaact cggtaggcgc aacggtgccc
    12361 tgcccggcca tttgcgtttg cagcgcggcg accgtggcca gttcagcatc tgatagcccc
    12421 atcccggcaa tcaccggggt gttggccacc acagccttca ccggtgtttt cgtgggagcc
    12481 gcggttggca gcaacgcctg agcagtcagc gccagcgctg tttgttgctg gttcgccatt
    12541 gaaaccgctg ccatggcagt ggctgtgttg gctgcgttaa tcagagcggc ctgttccata
    12601 ttctccgcgc gctggttggc taaatacttg ggagccaaaa agagcagcac aagcagcgcc
    12661 agcagaaaca ccactcccaa aatcacaatc acagtcagaa aaccccctct gccggatcgt
    12721 ttggcgggct caaccggaag atcctctgcg aatgcctctt cgtcaaacgg gttttcgtca
    12781 aaattattca gtgccataat actcctcctt catcgggtgc gccgatgaat cgatcatttg
    12841 aaaaaatgac gtccaaattc gacaggggaa tgatctccct gagattctcg ccaaaaacga
    12901 cccgggcagc ggctgctcgc agaccgctgg gaagcaatgt gacgcccggc agcaggcttt
    12961 cgatcctgcc tatccggccg gcgtacgcgc ctgtttgcaa cctcacagtt tgacccgagg
    13021 tgtacatcgc ctgctcctca taagcatgag cctctccctc gagcggtatg tacacttccg
    13081 gccgatcacc ggtcaggcga ttccacttaa ccgcgttaat cgaaacatcg cgaccaatat
    13141 aagtggctaa aaggctgcgt gaaagttcat ccatcccatg gtcgccaaaa ccgctcagga
    13201 tgatcaacgc gtaaggcagc tccagcgcgg ttgagatcag agaagaggaa atagacccca
    13261 ggatcaaacc gccaatcttc tcttgcgcgg cggcggctaa aaacgcctca gagcaggtac
    13321 cacctgcgag cacagcgccg cgagaagtca ctccaatggc tgaggtcttc aattcaccag
    13381 acaggctttc gtaatcagtg gttaatggcc cgcaacccaa taacgcgttt ccccaaaccc
    13441 cctggatgag ggcaccgtag cattgaatgg ttgcgccgcg ttcaggaatg acctgggtca
    13501 cttttccggc gagcccggct tgcattttaa tcctggtgct ctcggcttcg atgagcaccc
    13561 tgcccttgtt cacagagact accctgccct cgcctggcgt tcgaatcacg cgagaaaaca
    13621 acccacccgt ttcggcgatg atgtcgtttt tttgcaatac ctcgccaggt ttacgggtga
    13681 tcattttttc tgccgcggag agagttttag ccgatagcgc cctgacgaca tctaccaggt
    13741 aatgacgcgc cggaattgac gcttctgcca caatgtcctc aggagcgacc ctttgtcctt
    13801 ctctgacatt taccgagcca gggacaggta acagccggtt gcgatggata agtgttaatg
    13861 ggtttatttg cgtcagttgt gcgttcatat aaactcccgc gctatatgtt gcaataaatg
    13921 taccattatg ccgcgcccag caggaattcc cattctttga gcaactcgat tctcctgccc
    13981 tggtcttcag gcagcacgat tggccgcccg cgggcatcca ccaccacccc gcaaacccct
    14041 ccctgcacct tgatcggttc gcggatgagc gccagctctt ctatctccgc ccggcttgtc
    14101 agccgcaacc gcagcagccc agtcgccccc ggtgctaatg ggagcgaaat gatggaaccc
    14161 tgttttactt ccacctcaaa agaattgcca tccgcgtaat ccagccgcgc gtaaagcagc
    14221 gggctgcccg cgcgcgtttt gctggtaata ttgacaactg tcgccagatt ggaaaaagcg
    14281 gttgattcca gcagctgcac tgccaggagc gagttgtgag cagcgatcgt ccctaataag
    14341 ggtaaaaggc tgtgcttatc gaggatcaag gggctgatgc ccaggggttg gatgccatca
    14401 agtgccagca acaaagaacg caccgggtcg gccacatggc tgacggtgca accactcagc
    14461 acaatgggct caaaccgtaa agatggcatt atttcccgcg attccaggtc aatcatcatc
    14521 gcctgtaaca tctgccttaa taccgcctgt tcaatggcga tttcttcttt ggtcgcagcc
    14581 acagaaaggg ggaataaaga gcgattccaa agggcatcgc ttaccactgt gtcaggcaca
    14641 ggaaaggtca accagcggga gatatctttg atatcgcctt ttcgcaggaa tgcttcaaga
    14701 ccgctgccta tgccaaagtt gaacgtatta aggatagaca cccggtcatt actgtaggca
    14761 gccatagaat agttcatgcc gacatccagg cctaaaaccc ctttcagagg gtcgtactgc
    14821 cgacccagaa acttgatcat ccgcatgaat ccctggttct tcaactgagg tggggttgtg
    14881 cagagagggg atgtgcgttt gagccctcct atctcttcat tcatcttttc catgaccaac
    14941 gcatccagtt cagtcaaggc aggttcaaga ttctcccctt caaaggaggg ccgaatgttc
    15001 ggtgcgctgc gtacggttgt ataacgctca aacacatcct tcagctgagc gctcaacacc
    15061 tggtttccgc agtaaagaat ctgcggacgg gtgccgcgcg gaagcaattg cagcgcagtg
    15121 ctgacaagtt ctgctgttcg caagagagag cgggatgcgc cgccatccgt tcctcccgtg
    15181 aagatgacca gatccggcct gatagtcagc agcgtatcta actgctgaga gtaagaccgc
    15241 ctgtcattga tcccgatgga ttccaacagg ttagcgtatg tggcgttaca aagtttcctg
    15301 gctgaatcga gagacacatc gttcagtaat ccgactgcca ccacctggag cgatgagaaa
    15361 caagaggtgg tgatataaaa ttggtcgacc ccatcgcccg tgggcagcgc gggtaaaatc
    15421 agctcgccac ttttttcaat caatgtacgc ccgcaaatct cctggattcg gctcaagact
    15481 tcgtaaatag cgtctccaat gtccagaatg gggtaaaaag acgtggtcgg cgcagaactt
    15541 gaagcaatca gccgataact gttttcaaca atatcgaaca ggtgtccgcg cgtatgaacg
    15601 tcgccgattt caatccctaa aactgacatt ggttgcgcaa gcccgctttt cattttttct
    15661 atctcaccca aaacaacagt gttccgagaa gaaaatcagc ccgttcaaca agcgcagtaa
    15721 gtccggcggt tatcacaccg gcgaacaccg ccccaagcgt gatggcaatg aacaccctgc
    15781 caacggatga cagcagcctg atgctccaat gcgcctgtgg ttctttgcct ttcacttttc
    15841 gcgccgaaaa atgaaaataa aacaaagtcg ccagggtgcc cagcaggaaa aagatgccct
    15901 cgacgagcaa taacaggctc gtcggtcctt tttgagccct ggcgagatca acaaaaacca
    15961 atgccccttt tacctgccca aaaagggtgc caagcactgc tccgcctacc gccaccgccg
    16021 ccccaacccc taccagcaat gccatagagg ggttccccag ccggctcagg cggggcgaga
    16081 gcttcatgag taacatgccg gaaagcaaca aaggtatcaa cgctactcga gaaccttgca
    16141 gcaatggcac cgcgaggcgc gcgatcagca cctggtaaat gatcacaatt gtcatatacc
    16201 ctgcggtcat gccaatcagc acggcagaaa caaatctgaa gaagggaata tctcccaata
    16261 agaaactaaa cacgaccagc gtcaggatga agctgatgac cacccagata acctctgtgt
    16321 tgaaggacat caacccctcc ctttcttatg cgcggcggca ccggttgtgg tgacgagact
    16381 gacaacccct cccaggaaga tcagcaacgc ggcaatgaaa agcgcaaatt ggtacgaagc
    16441 gaaggttgtg gaggctttac cgggcacctg gcgtatcatc tgataggcga gcgcgccgga
    16501 agaacccgcc accaaccctt gaacctgtcc gctatcataa taaggttgag cgagtggtgc
    16561 ggcctgcgcg ctcaccacac caagcaggat tggtggtttt tcaattcgtc cggtttgttc
    16621 cacccaggcg cgaacgattt caggattatc ggtaagcagc aagatcgcgg cgaaatcagc
    16681 cagggttcgc gcgccagcga tgggggtggt ctcccaggcg ggttcaagag cagttgaata
    16741 aggcagcgcc gctcgaatat cgccaaccaa tgaaatcagc ccaatcgttc cgccaggcaa
    16801 atagccaaga ttgaccaccc gctcagaaaa caggtagcct gagacgcccg ccgaagcgcg
    16861 ccgcaactgt tcttcgagga tggcatatcc actggcgttg gtggacatca gcgcgagcgg
    16921 aacgccgtgg gaaaccagat gctggaacac tggttcagag ctcaaggtaa gttcgcccgc
    16981 cagccctcct tcaaactgcg cggctattaa taccggcttt ccagcgggga ttccctcgat
    17041 gacgccaagc gtgtccacca cctctttagg gaataaaact gggatgataa caggcagcga
    17101 gcgaaaacca taggggagga gcaccgccaa gaataaaatg accgccgcaa agatcttgag
    17161 ggttttcctt tgaggacggc ttttttttgt tatggctaca ggggaggttt cctcaatatc
    17221 tgggttactc agccggtaaa gcaactcagc gttcttctgc tgcatggggg tcacgactaa
    17281 ttcatggggg cgggaaggca tttttacctc cctgtcttcc agccgctgcg agcgcagtgt
    17341 gccggaaata cccgcgagga taccttcgcc ctcttcacca ggagaataaa gcgcatctgg
    17401 cgtagccggc gatacggcgt caagagggcg gatggctttc aaccaggcgg gcagcacgcc
    17461 atccgcaagc ttgttttctt ccttggagat aaagggtctg gctgtttggt ctttctgaga
    17521 agaatgtgtt tccaccatgc ccgcatcagc agacaaccat tcaggcagat catcctcgaa
    17581 gattggatga gccggttgac catcgcgcag ctcagcggtt tcaaacggtt tattcgccgc
    17641 gggtgaagtg gtttcaccct ctaccggttc acccgtaaat tcgccactcg cccggccacc
    17701 agtttcttcg aaccgtgaat cagcataagt atctgccggg gttgttgcct tcgcgtcaga
    17761 atcctccagc aaggcttcct cggctgtctt ttctgccatt actggcggtt cagcagcgcg
    17821 caggtttata atccattctg gcagcccgga gtcttcggta tccaacccag aggtttcacg
    17881 ggttttttgc tcatcttgct ctttcagcat ccggatgcgg gccagccatt ccggcgcttc
    17941 cccctcccaa ctttctccgc tttcttcagt gggttcaacc ctgccagaaa tttcagccaa
    18001 tggttccggt tcagggatct caccttcacc tggttcatcc ggagcaggtg gttctttata
    18061 gcctgggagt ggtgaacgac aataccaaca gcgtggtgag ctggcatcgt tcattcgatt
    18121 acaaataagg cagcgaatat ccgccataat caaccgttat agggcttttc tgagccaatc
    18181 aaaacgcgaa ttcccgttgc caggctacca agagcgatgc ccagcagcat ccctctggct
    18241 ccggccatcg gaacgcgatg aaaggctgag agcagattgg taaggatggg gatctccgta
    18301 gaaaaggcca gcactccgct gtttagtacc agaaatatga tcacgcttaa aaagaagaga
    18361 aaacccatcc agttgcgttg acgttgtaac aacttcagac tggcgaaaac cagcgtgatc
    18421 gccagcactg ccatcagtgt ggattcaaca ggaacctgaa cccgggtgac gagcttttga
    18481 aaaccagcgc tgccagggtc gagaaagacc cccgcaacca ctgtcagcag gaaggtcaca
    18541 atcaatgccc cgctgtacag tctgcgcggg tgcttgtcct gaatccgctt ccagtggaca
    18601 tgaaacacca ggtttaaaat cgccactaac ccggccacgc cgctaagggt gactgcccaa
    18661 tccagcagtg gagagcgggc cagtgaaatt cctgtgccag gaaagaaata ccccaataac
    18721 acgatcagac cagtaattgc ggcgatcagg taattcagta ctttcacgcg atacctgcca
    18781 gtttgagcag aagacctaaa agcaatgctc cgctaatgat caggcgcagc aggtcctgcg
    18841 tttttaggct ggccacgtgt gaagagcggt gaaccaggta agccggaacg gcgtacagct
    18901 cttccccaag catgacctca cgggcagtgg ccaagaaaac tgactgcgcg acaattgagt
    18961 cactggctgc cagtgtaaat gccccatttt cctcagccgc tgtggtcaaa aaaccgacct
    19021 cagccccata actgcccata aacacattgg tcctgacggc gggttcattc attacctcca
    19081 gggtgcccag cacataagcg aacggcgtgg cgcccgccat tcgcccatga tctgcatcaa
    19141 acatctcagt agattgggtt tctccagcaa ccatgcgcaa cacatctttg ctgagcaaag
    19201 tgaacccgcc attaccgctg gtgcaaacag gaggttggtc gctggcggaa cttaattgac
    19261 cgatacgctg cagcgtatct actccggcca gcccagaagg gttgctggct tcgttgagat
    19321 cgccgttccc cagtgatatg tgcacccggc tgccatcttc cacagaaagc cccaccgcac
    19381 tcctcaagcg attgatggca gtgatacggc gaaaggtcgc ccgtccttcg gatgatttct
    19441 tccggtaaga gagaaaaatc atccatccgg cggcgaaaat caccaccagt aaccccagga
    19501 taggttcagc cttcacgcgt cctcctcgcg caggtgctcc gcgaaggata gcgcaatggc
    19561 cgggtcttcc agcatgtcgg cgaacgcctg ccaggaagca gcactgtggt tgaagaatgg
    19621 cgcaaccgct agcattgcct gcgagatgaa gtggcgaaat attcctccat cttcaagcag
    19681 gcttaacatc aaaggtgtca gcccgtgacg gcgaaacgta tcttgccatt gtgaccagaa
    19741 tcgaggatcc atcgctaaca atagagtata gcccctttca tggctgggtc attttttaga
    19801 tgcaggattc aggccagatt ttcgttcaag cgacagcgct tcaaaaaaag ttgtacaatt
    19861 aatgttgacg gaaagcttaa gactcattct tcaggagcct gctatgccct ttttcttcac
    19921 ctggttcaag ccccgtcctt attctgcggc gaacagccgg gaagtgcatt ctcttctgga
    19981 agaattgatc cgtattggga ttaaggaaga ttacctttca gaaatacccg gatacggtta
    20041 caacagccag tgccggcaca tccgcacccg tgaaattgga aaaagactgc atgagcttgg
    20101 cggaaacgag ttgatgtcat gggcattcgc cagggtgcgc aagcaagccg gcaaagtacc
    20161 ggcttctcac ctcgagtacg cctggaacga tatcggcggc tggcagccct aaacagaccg
    20221 tcagcccaat cgtaatcagt gattgagggt gttgttcatc acccgcatga tgcccgtcat
    20281 gcgggttttc cagctgtaag cgttcgcggc gctcttcgcc gcgctggcta atttctcccg
    20341 ccgggtgcca tccgtgagca attccgcgaa cttctcgcgc aggtcagaaa aattctcgat
    20401 ttcgaagaaa gccgcggtct ggtcatttaa aacctcacgc aataccggca ggtcgctggc
    20461 aagaatgcaa cgtcccgcgg ccatgtactc aaagagcttc attgggctgc tgaccgctga
    20521 aatatcaccg ccaccgctac cgccgaaagc ccgactgtat ggcatcacca agatatccgc
    20581 ggccgcctgg tagaggggta actggctgtt agggatgaaa ccggtgagtt gcacgttatg
    20641 aatatcagcc tctttcaacc ttgcgcgcca ccactccacc tggtcatcat ttccaccaat
    20701 ccataagaaa cgcacctgcg ggaaagcgcg cgccagttgt tgcatcagct cgagcccgcg
    20761 cccagggtaa aacccaccgg aatagccggc cgtcatcgct tcttcgaggg ctaacttttt
    20821 tcgcgcagcc gccggtttgg gcaggtcaga ataccgctca agatccactc cgtcaggagc
    20881 aataaggtat tcaccaaccc tggcagccac cccaaagcgt tgatcagcaa gtgtttttaa
    20941 cgccgaggta atgtaaacga ccagttttgg ggctttcatc cgcaggatgc gccgataaat
    21001 atgcgggccg aaagttcccg acgggaggtc gtgcatctcc aaaacgaaag gcatcgcccg
    21061 taacccggcg aacaaagcca cccataacat acgcgtgtac accagctgcg cgccccaacg
    21121 tcgcgctgat ttgacccctg ccagggagaa gtccagccgt ttcaacccgg ggcgggtcgg
    21181 cagcgtgatg atttcgaagg gttctatcaa tccatactgg tcagcgagtt cctgccaggc
    21241 aggaggttcc tttcccggga ggtaaagaac cacttcctcg ccattttgcg ccagcgcctg
    21301 tgtcaccttc atcacctgga tgctgtgggc gcgggtagac gggacctgcg cggtggagat
    21361 acaggcgatc ttcatctcat tctcctttga gatccgcttt ttcaccctgc ctggcttttc
    21421 gtaaaccgat gaacaccaga agagcagtgg aaatgatcag gtaagcagat aagagagcgc
    21481 tttcgccgac gatcccccag cgcggcacca cccaaaaagc caatgcgatc ttaaccaacg
    21541 ccgcgctgaa caagacatag agcgggatgt tggcttgtcc gaaagacagc agcaaggagc
    21601 ggttccagaa gaagacattg gcgaagccaa agcccagcag cagaaccatc gtcgccggga
    21661 atgcggggac aaacgcagcg ccatagaaca gctggatcaa ccacctgcct gcaattaaca
    21721 tcaccagcag taccaacccg gtccatgaag cggaaatcag ggtaacgcgc ctgagcagcc
    21781 tgcgcaactg cgcccatgcc cgtgagacaa tactgcgggt catctcgggg taagtcgtgg
    21841 taacaaatgg ggtgatgggc atcattaaag ggttgacgat agaaagagcc agtttataca
    21901 gccctacaga ctgctgatcc aggaaaaacc cgacccacag cggttcgctt tcactggcaa
    21961 tcattttaac tgtgccgctg agattggtgc taagggtgaa atgagccagt tctttaaatg
    22021 ggggcagtag cgagagaggg gttttccacc atccaggctg taaatggcgg ggcagataat
    22081 agaaagcgag gatagccggg ctgataccca gcaggatctt ccctaccagg taagcgctga
    22141 gcacgaagat gattccgcca tgcagcaagt aagccagcag aatgagcagc gctgtgacca
    22201 cactttgaat cacattgatc atggcctggg tgcgaaaatg gttggtaatt tgcagcactc
    22261 cgatagaggt ctctgagacc atattcgcca atatggacac cgcgaaaagc tggatcatcg
    22321 gcaaggcctg catatctttg gcaatatatt tggcgcccag tggagcgagc aaaagcaaga
    22381 caaaaaacgc cacaaccgaa gtgaaccctt cggtcagcat cgccacttta acaaccgcgc
    22441 cggctttttg gcggttattt tcagtcaact ccttacccag gtagcggatg atgaactcgc
    22501 ccatgcggaa agaaaaaacc cggttcacat tgctgacaaa agaggtgatg attcccacca
    22561 acccgaaggc agccaccccc agcaggcggg cggcaaaaac gctttgcaca agcgaaagca
    22621 acaaaccaaa tgaagttgaa ctgaacaggt acccggtatt ttttaaaacc cgggtgagaa
    22681 tgtggtcttc aacaaggagt ttacggatcc tggcaaacag cgagttcatg ggagggggat
    22741 gtcacgagcc cacgcgcgct gctcaaggta ccagttcacc agctgaggga tgccttctgc
    22801 cagcagaacc ttgggttccc agccgagcag actgcgcgcc ttggtcacat ccgcccagtt
    22861 ggcattcaca tccgccgggt gccctgggat gtaatgcacg tttgcctttt ttcctaccag
    22921 gtgctctatc cgctgtaaaa gttcgttgat ggtgatggtt tcgtgtccgc caaggttcac
    22981 aatctcgtac cccagcggtt ttaacgcctt aatcacacca tcagcgatgt cgtccagata
    23041 cgtgaaaccg cgcatctggt tgccgtcacc agaaacacac acaggttcat tctccacgat
    23101 ccaacgcgta aagcgataca tcaccatatc aggccggcct gccgggccgt aaaccgtgaa
    23161 aaaacgcaca acggtcgtgt ctaacccata cagataatga taggtatacg ccatcacttc
    23221 ggcagccttc ttactggcag cgtagatttg cagcggatga caggaatccg cgttctcagg
    23281 cgtgggcagc ggagggttgg cgccgtaaat cgaagacgtg gacgccagca cgagttttgg
    23341 cgtcccgctg cggcggcacc attcaagaag gttcagcgtc ccggcggcgt tcgtttgcag
    23401 gaacacctgg gggatttcaa ccgcctgccg tacacccgct cgagccgcta ggtggatcac
    23461 cccctggaat gatgtaaatt cgcccgaaag cgattgatag aactccggtc tgcagatatc
    23521 atcgcggaaa aagcggaaac ccggcagccg ggcaagttgc tgcaaacgcc attccttcag
    23581 gcgcggatca taagagtcgt taaggttatc gacccccacc acctcgtgtc cctgggcgat
    23641 aagctgctcg atgaccctgc gggcgataaa cccggcagcg cctgtaacca ggtagcgcgc
    23701 cattagagtt ttatcacctt gggagatttc acgcccagct tacccaacgc gttacgggta
    23761 tcgataatta actttgcgga attaaggata gccgcgtaat cgtaaacctt atggttcgta
    23821 atgatgacca cacaatccgc ttcctcaacc gccagcatca ggtcagtcac cgattcaatt
    23881 tgccattcat cctcttgcac ccggggaata tatgggtcat gataactgac cagcgcgccc
    23941 ttggcctgca gcaggcgaat aacatcaatg gcgggcgatt ctcttagatc atcgatatca
    24001 ggtttatacg ccacccccag aaccactact ttgctcccct tcattggctt cgcgtgttcg
    24061 ttgagcccat cctgtaccct gccaatgaca aaacgcggca tgttggtgtt aatttcagag
    24121 gccagttcga taaagcgtgc ggtgtaattg agagagcgca ttttccatga gaggtacatc
    24181 gggtcaatcg ggatacaatg ccctcccaga ccaggcccgg ggttaaagcg cataaaccca
    24241 aatggcttgg tggcggctgc gtcaatcacc tcccatacat ccactcccaa tcggtcgcac
    24301 atcagggtca attcattgac aaagccaatg ttgatcatgc ggaaagtgtt ttcaagcagt
    24361 tttgccattt ccgcaacctc ggtggaagaa accggcacga cggtatccag cgccatgctg
    24421 taccaggccg tagaaacctc ggtgcatttt tcggtcatgc cgccgataac cttgggggta
    24481 tttttggttg tccaatcttc ccgcccagga tcaacgcgtt caggcgaaaa agccaggaac
    24541 agttcctcgc cgatcttaag cccgcttttc tccgtcagcg caggtaaaac cagctcacgg
    24601 gtggtaccag ggtatgtgct ggattccagc accaccacca taccagggtg cacatacggg
    24661 gcgattccct ccgccgcgct gatgatgaag gacaggtcag ggtcgcccgt gtggcgtaac
    24721 ggggtgggga cgcaaatcgc taccgcgtcg atctctttca ggatggcgta atccgtggtg
    24781 ccgcgtaact tgccatcggc caccagctgc ctgaccacca gcgagggtac atccaggata
    24841 tagctttctc cgcggttcaa catctccatc ttttgttcaa ccgggtcgat ccccgtcacg
    24901 gcaaaccctg cctgggcaaa aaccgttgag aagggcagcc ctacataccc catccccagt
    24961 acagccactt tggcagtctt tttcttaaac ttttcaatca actcttgcgc gtaatccata
    25021 taccaccttg agaattcaaa atagcgataa ctgttcgggt ttttcttcga tctctttcac
    25081 gccttctgcc gaggtgcgcg cctgcgcggg ttggcgtttc gctgcagctc tggcgctggc
    25141 aatcgcggct ggtagtgtcc gaaaatcgtt gatcagtgtt gatttgagcg aaggctggtg
    25201 cagcgcggct gccgggtgat acattggtac aatgagacga ccacgcaccc agtaagtttt
    25261 cgcgtgaacc tcgctaatcc tggcgttcgg taaaaatttc gccattgaaa acctgcccag
    25321 ggtcacaatt acatgcggat taatcgtttc gatctggcgg tcgagaaagc gcgcacaggc
    25381 cgccaactca tcaggcaagg ggtcacgatt gttaggaggt cggcatttta ccacattggc
    25441 gatgaaaacc tgctcccgcg tgtagcccgc ttctcctaac agttcatcaa ggaatttgcc
    25501 cgcctgaccc acaaacgggc gcccctgttc attctcatga aaaccggggc cttcgccgat
    25561 aaacattatt tcagaagtgg gcgaaccttc accagggaca gcgttcttac gtgaatggta
    25621 cagcgtgcat gcctggcagg tagccacctg ggcggctatt tctcgaagta tctcagcggt
    25681 gtccactcac ttcctccact tggaaaatca ccaaaaagta taaaccttat tttaccttac
    25741 tggaagcgct tcaggttctc caggttcaat ggcgcaagat ccatcagtat cgcgtcttcc
    25801 tggttgtctt tgtagtaatt acggcgcacg gctacctcac ggtaaccgaa acgttgatac
    25861 agcgtgatgg ctgcctggtt gctcctgcgc acttccaaaa acgaactgtt cgctcctgaa
    25921 tgataggcat cgattaatgt gtgcgccagc aggcgttggg cgatcttctg acggcgatac
    25981 gcaggcgaca ccgccagcgt ggccacatgc acttcatctg cgatcatcca aaccacgata
    26041 atggcgacca gcagcggcgc gccgctctcg gagagcagtt ctgccaccca gcagcgggaa
    26101 acctcgttgt tctcaagttc aaaccggaaa gaccgttcag gccagggaag agaaaacgag
    26161 gagcggtcca gttcaaaaac ctgcggcagg tcttcgctct gcatccggcg gatgcgcaaa
    26221 gtgccagaca cttctgtttc gctcactcct gaatcatccc tgccacgtgc agatagatgg
    26281 gcgccaacag cgcagggtcg tccacctctc cttcattcca gcgtttccag gcgagttctg
    26341 ccagcatgga agggcgccgc attgccagcg cggggggcgc gatcatggcg tttttccact
    26401 tgcggccaat cacctgacgc tctgaagggc tcatttcacc gcaaatgcgg gtcggtccgc
    26461 tgatgcggtc agatatttct tcagcggtca tcacgcgcgc ctcatcctgg ggctgccagc
    26521 agccgctgct ggaagaaaac tcctgcactg ccaggcgcga ccttcccgcg ggcaacacag
    26581 tgagcagggg ttcagctgcc aagggttgct gtgctgcttg aaattcaaag gttgggatgc
    26641 caagcagggg aatatttaaa gacagagcca accctttggc cagtgccaaa ccaatgcgca
    26701 ggctggtgaa agaccccggt cccagcgcca ccgcaacagc cttaagttca gcggggctca
    26761 ccatgcagcg gttcatcagg tcctgcaccg ctggcgccag ctctactgtg tgatgagaag
    26821 ccgtgcgcca aatcatttcg ccgataagga caaattcatc gtacaacgcg atgccgctgg
    26881 tttgtgttga ggtatcaatc gcgagcagca tctaccctcc aaacgccaat cgcttaaagt
    26941 cgtccagcat ctttttatat cgctcgccga cagggataaa caacaggttg cgctgctctt
    27001 cgttcaacca ggcgtatttg atcaccagcc tatcggctgg cagcactgac tcaatccgct
    27061 cagcccattc tgccaccaga attccgccgc tgagcatatc atccaggtca agatcaatgg
    27121 cttcatcaat agaagtaagc cggtaagcat ccaggtggtg catcaggccg ccgccagctc
    27181 ggcgatattg attaacaatc acgaatgtcg ggctggtcac tgcgtccacc gagccccagc
    27241 catgggcgag cccctgcaca aaagtggttt tacccgatcc caggtcgcct tccaggcaga
    27301 gcaaatcacc acttttaaga agggtgccca tgcgcatgcc caaccgacgg gtttgctcgg
    27361 ggctgcggct gacaaaatca atactataag cgggtaagat aggcatcggt ttaatgatca
    27421 gattgcgcga taggctcact ggtaaacatc ggatggcggg ttttcttgcg gggcatcaat
    27481 cgggaggtaa gttttcatac ggcgggtcaa ttctcggcgc gtcagcatca cgcgatgaga
    27541 acaggtatca cactccaacc caatatccgc gcctaacctc acgattttcc aggtataacc
    27601 cccgcatgga tggggtttcc gcagcctgac cctgtctcct aatttcaact caggaagcat
    27661 gctgcgatta taacccatgt tataattttc aaactcacca caactggctg ggcttaagag
    27721 gaaattaccc atatgttgaa cctggcacca agcctgttat tttcccgcat actcacattg
    27781 atcatcgcgt taaccatcca cgaattttcc cacgcgctgg tggctgacca cttcggtgac
    27841 gttaccgcgc gcaacgccgg caggctgacg cttaacccat taaagcacct ggatgtattc
    27901 ggttctctga tgctgctggt ggtgggtttt ggctgggcaa aacccgtgcc ggtcaaccct
    27961 tacgcgctgc gccgcaggtc agagtcggcg ctgatgtggg tatcgctggc cggtcccgcg
    28021 tccaatctgc ttctggctat cctcgccggg ctggtgctgc gctttcggat ggtcccctgg
    28081 cgcgcatctg ccgcgggctc catgctccca tccccggctg aattcctctt caccttcctt
    28141 tccatcaacc tgctgttgat gttgttcaat ctcatccccc tttcaccgct ggatggtgaa
    28201 aaggtgctct cctacctgct tccctatcgt ctacagccag ggtttgaaag catccagcgc
    28261 tacggcccgc tcttgttaat ggtgctgtta tttgttcttc cgctggtggg aatcgacctt
    28321 atccgttggg tgatgtcgcc cgccctaacc cagcttcaaa agttcattat aggagtcggt
    28381 tgatgagccc gcaaatcgca ctcattggtc tcaaccaggt tggcgcctcg atcggcctgg
    28441 cgctgggcgc ttacaaggaa aatattacgc gggtgggtca tgacgctgac agcgcccgga
    28501 tgcgcaggct ggaaaaagaa ggcgtgtgcg ataaaaccca tacccgactg cccgacgcgg
    28561 tacgtggtgc cgatatggtc atcctctctc tgcccactga cctgctgcgc gatgcgctgg
    28621 ggctcatcgc gggcgaggtt tttccacaag ctgtggtggt gtgcatcagc gcggttgcca
    28681 gggtcgctta ccagtgggcg cgtgaacttc tgcctccaca gcagcccttt atcgttctgc
    28741 aacctgtgtt gcatcctgcc aggctgcgag actgggaaga gaattcgagc aacccgtatg
    28801 ccgacctttt tgaaaacaca cacatgctgg tggtaaccga ttcaaacacc caggaaaaag
    28861 cctttcaact ggcaaatgac ctgtgcgcgt tactgaaagc caaaccgtat tttagtgaac
    28921 cgctggaagc tgatggcatc accgcgcgca tcgagcaact gccaaaactg accgcggtag
    28981 ccctgctttc catgctggtg gaaaaacccg gatgggatga cgcccgcaag atgaccaaca
    29041 gggctttttt ccgcttcgct tctctcaata ccttgtttga cgaacaggag ttcttcggtg
    29101 tttccaattt ggtgaacaga gaaaacatca cccgcgcgct cgatgaattg tccgcgaccc
    29161 tggctgagat tcgccagatg atcgatgctg gtgatgaaga ggggttgcaa aaaatgtatc
    29221 aaaccgccag gcaggggtac gaaacctggc tcacacaacg catgagcggg gaatgggata
    29281 aggtggaaaa accccccgaa gcgctggaac gcggcatcct tacccgcctc tttggcggca
    29341 gacctgtcaa gaaataaaaa ttggagatga gcttctattg ttacattgtc gaatgcgctg
    29401 acggcagtta ttacaccggt tggacccttg accctgcccg tcgtgaacgg cagcataacc
    29461 tggggaaagg agcggcgtac acgagagtac accgtccagt tcggttggtg tatatcgaag
    29521 aacagcctga ccgctcaaca gcgatgaagc gtgagcgcgc catcaaggcg ctaaatcaca
    29581 cgcaaaaggc caggctgatc agcaagttta ctgagatggg cgatgaccgt ggcgacccct
    29641 gaaggtaccg tcatctcccc tggcagagtg aacctgctgg gtgagcatgt tgattataac
    29701 gacggcgttg tgctgccagc cgctattgac cgttcggtga agatcatctt caagaagaat
    29761 gacagccgcc tggtatcgct caaagccgtt gacctgcaag accaatgcgt catccacttg
    29821 agccatcttg aaaagaaaac gtcgcaagaa ggaaaaccat tacccgattg ggcgttatac
    29881 cccgccggcg tgatgtggag cctacaacgc cacgggcttg atctaatcgg attcgacgcc
    29941 caattcagtt ccaatattcc catcggcgca ggcctgagca gctccgcggc catcgaagtg
    30001 gggttcgcgg tggtctgtat gagcctgtgc ggctggcagc ttgaacgcct ggaactggcg
    30061 aagctctgcc aacaggctga acgcgagtat gtgggtgtaa attgcggttt gatggaccag
    30121 ttcgcctgcg cttgcggagt ggaagatcac gtggtaatgt tcgacacgcg cagcctggct
    30181 tctcaaacac tgccgctccc tcccgctact acgctggtta tcgcggattc ctcattacgg
    30241 cgttcacttc tcacctccgc atataacgac cgccgccgcg actgcgaaag cgtggtcgct
    30301 tattttaatc aacttgaccc atcaatccgc tcactgcgcg acctggggga ggaacgcctg
    30361 ctcgcgcatg agcacgcgct cccgccagcc gcgttcaggc acgcccggca tgtggtggaa
    30421 gaaatctcgc gcgtcaatca ggcgattgat ctattaaagg aaaaagacgc caggggattt
    30481 ggtagattga tgttttcctc gcatgattcg ctgcgcgacc tttttgaagt ttcgcgcccc
    30541 gaactggaca ttctggttga tattgccgcc gggttcgatg gctgcctggg gtcgcgtcta
    30601 acgggggcag gctttggcgg cagcacagtc agcctggtgc ttgatgagaa aagtgatgcc
    30661 ttcattcatc agttaagaga cctctaccaa aaacagaccg gattgcaggc ggtcgttttt
    30721 tccacccgcg ccagccgcgg tgctcacctc aatcctgatt agaaaccacc ggatatacgc
    30781 catgctgaca tctacaaatg atcgcgccat tgaaacccac tcgctggtga agacctttca
    30841 caccaaacat ggcttcctca cacgcaccac gcgctctacc gaggcggtgc gcggtatcac
    30901 cttccatgtg aacaaaggag aactcttcgg tctgctcggt ccaaacggcg ccggcaagac
    30961 caccacggta aaaatgctct ccactctgct cattcccacc agcggcagca ttcatatttt
    31021 cggtcaggac attctgcagc acaccaggga tatacggaag cacatcggtt tcacctttgg
    31081 cggggctcac gggctgtatg ggcggctgac cgcaatcgaa aatttgcgat atttcgctga
    31141 actgtacgcc ttgccccccc gtgaaaccca taaccgcatc acggagcttc tggagctggt
    31201 gggtttgcgc gatcgcggga atgaccgggt cgaaacatat tccagcggca tgcagcagcg
    31261 cctgcacctg gcgcgtgttt tactgcacga ccctgagctg atcttcttag acgaacccac
    31321 tgtgggcatt gacccggttg gagcacgtga actgcgctcg actatcaaac tgttaaaaaa
    31381 tcaaggcaag accattctgc tcaccacaca ctacatggca gaagcagatg agttgtgtga
    31441 tcgtctcgcc atcatcaatc acggtcaaat tatcgctatg gatagtcctt cttcattgaa
    31501 gagcaaattg accagcgatg cctttgttga gttaaaaacc agcggcgaaa acctgccatc
    31561 cgtaaaaaac gtgctcgccc agctcgaccc ggattcagag gtcacctata accagcacgc
    31621 tttcgtctgg gaagtgaata tccgcactcg gcaaccaggt gtggtgatgg aactgctcgc
    31681 tcccctgctc agcagcaacc tgatacagac gctgggggtc cgcaaccaga ccatggagga
    31741 tgtttacgtg tccatcattc aaaatggagg ggcatgatag ggaattttct caggttggga
    31801 attcgccaga tgaatgtgca actgcgcatg cgtgtgctca gcctgtggag cctggcttta
    31861 tttttcacac agccggccat tttcagcgcc atcggcatgc tgctggcgcg cgccgttgga
    31921 aacacccgtc ctgacctggt gtacaccgtg cttggcgccg gcatcatggg tatgtggagc
    31981 ggcttggtgt tcacttccac ctacgatatc cgcgctgaca ggcgcgaggg cactttggag
    32041 ttgatcgtgg gcagccccac tccgctgcgg cgcgtggtgg gatttcgctc gctgctcaac
    32101 atcctggcgg ggtcaaccag catgctcgcc gcggttgccg cggctttatt tatcttcgat
    32161 tattcattca gcggtatcaa tatccctggt gtcgtcatct ccttctccct gcttttactg
    32221 gcactgtggt gcatgggcat cttcctggcg aatatactcg cctggtcgcg cctttccggc
    32281 actttcgtcg atttactgga gatgcccatc gcgcttttgt gcgggtttat gtacccgatc
    32341 agcgttttgc ctgcttggat gcagcagatt tccgctgtct tccccatccg ctgggggctg
    32401 gagactttgc aggatgcgct aaaaggctcg cttatcgaca gcgccttggt aaacaaatgg
    32461 ctgatcgcgc tgggggtttc attcgtctac tatctgctgg cgcgctggtt tgataggaaa
    32521 gtgcacgatc atatccgcat caccggggag ctgcattcga tatgatcaag cggcttgggc
    32581 acctcttccg gctgtacttt ttccaggcat ggttttcata ccgctccctt tttgcctgga
    32641 gcacgccatt cagttacgcg accagcaaat tcggctttcc cttcttcgcc atgttgttct
    32701 tcgtgttcat gggtaagttt gtcggctttc tggatccaat gttcatcgtt attggcaata
    32761 tcttgatgat gcctaccact aacgggttat acggtatttc gatgaccatc gggaacgagc
    32821 ggcagtttgg cgccatgtct tacctgttgg gcagcccggc gccgcgcgct ccgctctttt
    32881 tggggcgtgc cttttttcac atcctcgacg ggtttgtgac cgtgctggta gccctgccca
    32941 tcgcaatgct catcttccat atcgacttcg ccgaggtcaa cctgttcatg ctcgcctgct
    33001 gcctggtgct gctatcgata accaccaccg ggctgggatt catcatgggc acggtcagcc
    33061 tgctcagccg cgacggctgg atgtttacca acaccctcgg tcagttgctg tacattttga
    33121 ttggggtcaa cattcccatt gaaagactgc ctggtttttt acagccgctt tcgggatacc
    33181 tgcccatgac gcgcggaatt ttagccaccc ggctggctct gggtggagcg ggttggtcag
    33241 aagtagccag cctgttggcg ggggagattc tggtggctgc tgtgtatatg gtattcggct
    33301 acacgctttt ccggctggtg gaaagagcca gtctgtcgac tggctctctg gactccattt
    33361 aatgaggatg cgcaggcaaa cagcctgtgt tcaatccgta aacaccggca ggtgacccag
    33421 ggaaataatt tcgtgccacc tggcccgctc cccttctgtg agaatggcgg cttccgccac
    33481 cacctcagcg ccggctttct gcaccagcag gcgcatcccc tgcaaagttg aaccggtgct
    33541 gatcacatca tcaacgatta cgaccttctt ccccatcatc agctcgcggt ctttttcatc
    33601 caggtacagg tattgcggct caccggtagt gatcgaaagg gtttcagcct taagcgcatc
    33661 tcccatatag ggtttataaa ccttgcgcag caccacgtaa ggtttcatcg tctccgccgc
    33721 cagcgcgtgg gcgatgggga tgcttttcgc ttcggctgtg agcagcaaat cataatccac
    33781 agcggtcagt ttttcagcca gggccgccgc gcaggattgt acaagttcca tatcgcccag
    33841 gatattcaga atcgcgattt ttagcccggg tttgatctca aacaaggtca ggtcacggtg
    33901 cacgccggca atttcgatgg ggtaggtttc tctcttcttc atatgacatc tccaacagcc
    33961 tgatatggga actgatgcga gcaaaagttt atcacaaatt tttatgtaaa aacaagggat
    34021 tcccgcgctt cggtttacgg gaatccttat aaaggggtga cctatggtag aatcatcgag
    34081 tgccggggac gccgaccccg gcactcttta ctaaggagga gaagaaaaga agtcgccctg
    34141 taaatagtgt accagtgacc ctctcgcact gcttgactct taccctacga ttaccctacg
    34201 aaaatgatgg tcgcaaaaaa gcgccatttt cgctgtaaga aaccatattt ttcaacgaat
    34261 gatataaact tataaacatg cctgatagaa ttcaatttca aagccctatt ggaatggtca
    34321 ctgttctggt ttgcagagat gctgtctcag ccgttcaaat cagcataaac accgggaaac
    34381 ctcaccctgc aatgacgaca aacccggttt tatgcgcatg ccgacaacaa atgggagaat
    34441 atttcgctgg tcagcgtaag gctttcgacc ttcctctgga ttggcagtcc atccgggggt
    34501 tccagcgtga ggctcttctg gagttattga agaccccgta tggtgaagtg cgcacttacg
    34561 gtgagatttc cagggcattg gggaagccag ccgctgcccg cgcagtgggg atggcactgg
    34621 cgaaaaaccc gttaccgatt ctcatccctt gccaccgcgt ggttgctgct tcagggcatc
    34681 ttaccggttt ctctgccggt gatggcctga actcgaaagc gtggttgcta caactggaag
    34741 gacatcgcgt tgtcactgaa aagctggctt aaatttattt tgcttggcat cgcctggggc
    34801 tcctcttttt tctggattaa gatagggctg caggaagttg gtccttttac tgtggtattt
    34861 ttccgcattt tcttcgcgct aatcgggctc accgtttttt tcttgctcaa ccgtcgtaaa
    34921 cccctgctaa aagcctggcg gttgtatctg ttcctggggg tgttctatgt agccctgccc
    34981 tttgcgctgg ttgcgtggag tgaaaaatac atcacctcgg gtctggcctc catcctgaac
    35041 tcgatgcagc cgcttgccac ctccctcatt gccgcggtgt tcattcaaga agaaaggctc
    35101 aacgcgcggc gcatcgttgg tttgttactt ggctttggcg gcgtactggt actgatgtca
    35161 gataaactca gcggagaagc catcaatcaa atatacggta tcatcgccat ggtggcagcc
    35221 gttttctgct acgggggcag cagcgtattt gcccgcatgc acaatcaggg ggtgaaacct
    35281 gaagatcagg cgatagggca attcgtcttt ggtatcctct tcatcttccc aaccatgtta
    35341 gcggttgaat ctccgttcat tatgccagtg cgaccagtct cgtatctggc attcgcctgg
    35401 ctgggcttga taggatcttt cttcgccgcc atcgcctggt actcactgct ggacgagatt
    35461 ggtccctcgc gggtgagcat gaccacttac ctgttccccc tggtgggtgt tctgctgggg
    35521 gcgatcctcc tgcacgaagc ggtagactgg cgggtgatcg tagggggtct gatgatcctg
    35581 ggcgggatcg ttatcgtcaa cagcggaaaa agaaacaatc aaaccccagc ccaggttcag
    35641 gccgacccat ttgaaggata aaatgatgga aaaacaagaa ttccgcgctg ggtatgtagc
    35701 cgtggtcggc aggccgaatg tcggtaagtc cacgctcatc aacgcgctgc tcggtcagaa
    35761 gatcgccgcc gtctcgccaa aaccgcagac cacccgccgc aaccagctgg gcatcctcag
    35821 tctgcctgaa gcgcagctta tcttcgtgga tactcccggt ttgcatgatc cccagcacaa
    35881 gctcggcgat ttcatgaacc aggctgcggg cgaggcgctg cgagacgcgg atatcctcct
    35941 ctggctgtta gacgccagcg cgccattcac cagtgaagac catctcatcg cggaaaaact
    36001 gggcggtatg cgcagccttc ctccggtatg gatggggtta accaagaccg acctggtgaa
    36061 cgaaaaaacc cttgaagaac gcaaacgcca ggcggtagaa ctgtatccag cgcaggacgt
    36121 attgactttg agcgttaaaa ataaagccag ccttgacgcc ctgctcgaag tgatgatcag
    36181 ccgccttccc ctgggtgaag ccttttatga aagcgatact gtgacggatc tctatgaacg
    36241 cgatatcgcc gcagagctga tccgtgagtc agtgttgact ttcgtgcagg aggaagtgcc
    36301 gcactgcgtg gcggtgaaga tagatgctta tgaagaacga ccgggagaaa aggcttacat
    36361 cgaagccacg ctttttgttg aacgggattc gcaaaaaggc atcctcatcg ggaaaggcgg
    36421 ggaaatgatt aaaaagatcg gtactcacag ccgccaggag atcgaagaaa tgagcggttg
    36481 taaggtcttt ttagaattaa aggtgagagt gaacaagaac tggcggaatt cgccagacgc
    36541 gcttgaatta ttagggtata aacaggttgc aggttaaaac atgttaacct gggcgttctg
    36601 gctcgcgggt ttcttcgccc tgctcgactg ggccgctgtc tggaaaggtt ggaggaaacg
    36661 cctttacctg gccaagcccg cggtgatggt ttttcttatc ttttggacgc tgcagctgac
    36721 cggatggcga aatgagatga cctggttcgg cgcgggtttg gttttctcct tgattggaga
    36781 tatagcgttg atgcttaacc cccgtttctt catggtcggg ggaacttcgt ttctgttcgc
    36841 ccaggtggcg tattttattg gcttcaatac ttctcccgcg ccgctctccg gcggcgtgtt
    36901 cctggtggct gtgctggtag gcgcggctgc tgctgtgatc ttcaaacgca tccgcccggg
    36961 catcctgcgg gttccgcgcg gcaggcgttt tcttgctgct ctctgccttt acggcttaac
    37021 gctcacgctg atgctgcttt cagcgctgct gacgcttttc cgaccagaat ggcaatacca
    37081 gccagccatc ctggctgcca ttggcgcgct gctctttttt tcttcagata ccatgctggt
    37141 ttacgaccgt tttgtacgcc ggttcaatca cgctcagagc tatgtgcacc tcacctatca
    37201 cctggcgcaa ttcggcatca tcagcggtgc agtgctgcac tgcttgcgct aggtggtcta
    37261 cttcaaaggt ctgaacccct cccctaacgc ctcgtgcgtg accccgatga ccataaacgc
    37321 ctgcgggtcg atctctttga ccacctgttt taattgattg acttcagcgc gggtgatgac
    37381 gcagtacaaa acaggtttat ctgtgccggt ataagcgccc ttgccgctga gcaccgtcac
    37441 tcctcgtgag agcacagaca tgatctcgct ggaaatcttt tcatggtatg gtgagatgat
    37501 gatggcagtg cggaatatcc cggacccctc tgagatcatt tccgctgcca gcccgctgat
    37561 ataaatgacc accatgccat aaagcgcttt ttcccaagag aacgcgaacc cgcccgcgag
    37621 tacaacgatg gaatcggtaa tgagatacgc cgtcgagatc gatatcccca gtctgtggtt
    37681 gaggatgcgc cccaggatat cagagccgcc actggtgccc cctccgcggt agaccagccc
    37741 cagccccaca cccagcaaca ccgctccgaa aaccgcgttg agcacgttgt ctcgcgttac
    37801 gccttgcgcg gggatgataa ataccagcag gtctgtgaac aaagagaaag cgcctaccgc
    37861 gatggcggtg cgcaacgcaa agcgcggacc gcccaaaaac cgccatccta aaaagaagag
    37921 cgggacattg ccgatgaaca ccatcaaccc aatgggaata ggcgcgaaat gattgatgat
    37981 ctgggcggcc ccgctgattc cgccgctgac caattgacct ggcaccaaaa aaagccgcat
    38041 cgccagcgcc tggatcagcg cgcccagcag cactaccagg tactcgcgcg cggtcttcca
    38101 cgtgaattcg agcggcccga tcgcagaagt gaagcgaggc cataaggcag ttacagttcc
    38161 tttttgagca gtcattagaa acctcttcta tgaagccgca caattgtaac agatagacga
    38221 tgaaaaaatg ggggtttcgc ttgacggtag ttggaggtag tggtattatt gggtttcttg
    38281 atgcggggta gagcagtggc agctcgtcgg gctcataacc cggaggtcag tggttcgaat
    38341 ccactccccg ctactaa
//
LOCUS       DAOTLW010000014        38296 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1283_length_38296_cov_10.224157, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000014 DAOTLW010000000
VERSION     DAOTLW010000014.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 38296)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 38296)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38296
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1283_length_38296_cov_10.224157"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1438
                     /locus_tag="P5516_03725"
     CDS             <1..1438
                     /locus_tag="P5516_03725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HRT91579.1"
                     /translation="TVQGTPTFSWKAVPGAKYYELWVDEADGDFSDPVYKKSMLTTLN
                     HKPPIIMEGGYRWRVIASDLAGNESEGGEGWIVTVNPLKPVAPVLTGPANGVFLNDEK
                     TEIVLSWNPVTAYAQRYEFRVDDDPRFTSPAYSESSGNETSYLLTDTAAHLLDNTRYY
                     WQVRAVNSAGAETGAGAWSAVRSFTVDRTPPVNAPGLRTPANEASMATYTPALSVNSL
                     KDAKWYRFTVYQSGTCEANDGFISPDVTATSWAVPAAQALAYGKYHWCVQAVDAAGNA
                     SSWSEARVFTLTFQNQPGFAATTASATPGFSWYAVRGARGYRLEVKPVVEEGNTATRE
                     YSVYLGTVTSHVIPAIKALDPGAYQWRLRVYTAAGSEETPWRLLVVLPPAPVLDTPDN
                     GSVHPGVTVELNWFPAAGAARYEIQADNNNNFRTPEIDYEFTEGPVTTKEVTFPFAGG
                     FYWRVRAYNYLGNPGAWSAARYLRLTTP"
     gene            complement(1582..3435)
                     /locus_tag="P5516_03730"
     CDS             complement(1582..3435)
                     /locus_tag="P5516_03730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91580.1"
                     /translation="MRVRGAFSYLTGKGILLVCLTVLTVALLITNSVNIHPASAQDGP
                     DRVTWITVDDSQYDWWLARWSDNTIVCQFTTAHAGTPSELEIQKFCGDQVLNTWLTTP
                     PCTAGEEGKDLSSCSGLYLQNTRSSLSANEIMVNLPPPVVWITVNGSVLPGTTNKWEG
                     RPEVVLIGEEKLANESITRIHGVYGGTKFSCAGSRCPLPLWQTGEQGVMLTFWGESSY
                     GDTTEEYTAYIRVLPWADMPADNAGAPERSGYYVDIISSQWQAQQMSSCAAIWQSFPS
                     VEGLPAWLKNPADRASLSTSLPLHYLAAMLIQNGVVDAGDCPYGGLDYYASANQCGME
                     AAMQAVTLWQNQFDDEIIRTSIETGIPSILLKNIFAHESQLWPGIYHNAEEVGFGQLT
                     EDGAEAALLWNPEFYTQFCPLVLYEDTCARGYGNLSQEQQTTLRGALVQKTNATCPDC
                     DLGIDLTQANFSVKIFGETIVGNCAQVDRMLYNLTGASAGAVASHDDLWRYTLVNYNA
                     GPGCLWHALKRTWEAGNRLDWLHVAANLEGGCRAAVNYVMDISGGDTHPVMVYSTSLP
                     SPTITPTIVRATATLTKTPSFTTTPGTATPTGSITPTPSPTKTSAWGTVGP"
     gene            3574..4059
                     /locus_tag="P5516_03735"
     CDS             3574..4059
                     /locus_tag="P5516_03735"
                     /inference="COORDINATES: protein motif:HMM:NF012920.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HRT91581.1"
                     /translation="MQERAQGGLLEPQTYSISGSDLVALMEAVLAKNGTFRFTASGSS
                     MAPFIQTGDMVTISSLHKGRVGLGTVVAFKHPVGGHLVIHRVVGVAHGTFLIKGDNTP
                     HRSDGWIPSGCLIGRVLEITRNGRRVWLGLGVERYLIAFFSRWQWLIPLRGALAALRR
                     R"
     gene            4203..5915
                     /locus_tag="P5516_03740"
     CDS             4203..5915
                     /locus_tag="P5516_03740"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HRT91582.1"
                     /translation="MDNELEERSSLESILDYAQTLWRWAWLLLIVAIAAGAVAFYLTN
                     QKPRVYESSTRAVVNVVTGSNFYDAYSASFGAQRLAETYSQTMITPELLQSVSERLGF
                     EVTGKITVTPVENSPIFTIVVTDNDPQKAADTANMLITIFAEKVMKDQSSRYSELKQG
                     LEEEIARIDQNLTDINERLAILQIKEAELAEAEKRAAQMAAEGLEPIEVVRDPNDYVT
                     RAQLELAQSQYQSTRSSLFYNLQQIKLTEVQSATTISQIDKAMANPEPIQPQPFKSAG
                     LAAIVGLMVAAGIVFLIAFLQDEIRDPEEFARRWGVPVVGVITHYPSNQDKLITVAKP
                     RMSVSEAFRSLRTNLQFSGIDTPLKSVLVTSPSPSEGKSSVVANLATVIAQSEKEVVL
                     VDGDLRRPTLHKVFKLSNRIGLSDYFIREPEKMSGVVKKTKVNGLNIISSGSLPPNPS
                     ELLGSTKMHDVLGLLEKHFDMVLLDSPPLLAVTDALILAKSVDGVLLVVDPKKTKRGA
                     IRQAIEQLQRLDARLLGVVLNNIKIKRSSYYYNRDYYYSKQYGKSNEDGTHEEPAVEE
                     DKTR"
     gene            5931..6833
                     /locus_tag="P5516_03745"
     CDS             5931..6833
                     /locus_tag="P5516_03745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91583.1"
                     /translation="MTGEPNPAVKGTKTDKKRNDTLFCPFLGFEDDPTTALAYPAKLN
                     FCFRAKPAAPVSLAHQRDKCLTEFYVDCPVFQSENPIPLPESIRGERKSKTFQFNWVP
                     LVALIAVVIIGLVALLLTGVFTFRGFTPPLADISTATHTPAHLPVVPLSAEPTTTVEA
                     TATLVPTVTATWLIPGKHTPRALETPFGTSPQLVIHKVAAGEGFILLAEKFHTTADAI
                     KAINYQLPDSLWENTLLVIPVNTDDVAGLPAFSVVEIRAEGMTIESYAARMQLDVEML
                     KKYNDLPEGYSFQIGEMLIVPMTE"
     gene            6857..7360
                     /locus_tag="P5516_03750"
     CDS             6857..7360
                     /locus_tag="P5516_03750"
                     /inference="COORDINATES: protein motif:HMM:NF013607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91584.1"
                     /translation="MPNVLFVCSANRFRSVLAAEYFTSLLEKQAPSGEWVVGSAGIWA
                     REGVAPTKEALQWAAGKQLHIESVRSREISNALIVEADLIIVMTNGQREAISIEFPEA
                     REKLTLLSEIAAAQIYDIPDPIIESGEEPLQIAEEISTLIDAGFERILKRAAGRANNL
                     GAQLRGA"
     gene            7350..8372
                     /locus_tag="P5516_03755"
     CDS             7350..8372
                     /locus_tag="P5516_03755"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRT91585.1"
                     /translation="MEHKPPNAKRRLLWLLLVLLFLVSCGYLLWTNPLLGKRLPQFTY
                     EPLATFTAIPTSAALTLTPETVKTEPALATVEPEPTSVSPVCGQTQPMIILALGIDES
                     EQADMIRVVRVDWLKHQVVVLSIPRDFWVPIPGLEEHNISQFRINAAYGYGEYFNGRG
                     QGVVKFSETMYQNYGVTFDRYVVAHFNTFTELVDQIGGVDIDLEEPIGAYASAGHHHF
                     DGKSALLFVRQRIADLDAYRIQRQSAILKGLYEKLSKPEYLVQLPGLGVKFIKDKSVI
                     TDLRLQDVYTFNCFIREINRDSLVFIDIPYDLYTPMITNYGRHIKLPKPEAGVFIQNV
                     IKNGTE"
     gene            8705..9952
                     /locus_tag="P5516_03760"
     CDS             8705..9952
                     /locus_tag="P5516_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF438 domain-containing protein"
                     /protein_id="HRT91586.1"
                     /translation="MSELINNKQKRVELMKSLIRQLHQGMAEDRVRMQLETLLDEADY
                     NDVFVMEVQLIQEGIPAEVVQDLCDTHTRVLKKHLDLQETPQTIPGHPVHTFVEENRE
                     LTKVTAQIRHLMSLIAEKPAEEDVSEQMGEIHQLLNNLMDVEKHYQRKENLLFPYFEK
                     NNLPGPPAVMWGKHDEVRELLKTTLEGFQQVKSFSAGEAAAFNQFSVSMVVDAIDEMI
                     YKEEKILLPTALDILSEEDWYAIYLESDEGGYCLYVPQFTWTPEGDFKREIRNPSGVG
                     ARIQMPTGSFTLDELVALFSTLPFDLTFVDKDDSVRYFSPGTERIFKRTRAILGRKVQ
                     YCHPPKSVHIVNRIVKDFKEGKQDQARFWINKGGRMVYIVYYAVRDEQGGYLGTLEVT
                     QDVTDIRALEGERRLLEYDGAPK"
     gene            9980..10501
                     /locus_tag="P5516_03765"
     CDS             9980..10501
                     /locus_tag="P5516_03765"
                     /inference="COORDINATES: protein motif:HMM:NF020553.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HRT91587.1"
                     /translation="MEINAKTKLHNLLSTYPQLEEKIIQAAPAFKNLKNPVLRRTVGQ
                     LATVEKVVQIGGLDLTTFINLLRREVGQPELIPDPSSDNGKDKSAPGAKPDWTNGKIQ
                     FTVDGKELLAKGEVPVNHVNALLPQLEENKLILLVTDFEPIPMIEVLKKQKREVFHMV
                     DSHDAGKHLTYIK"
     gene            10823..11992
                     /locus_tag="P5516_03770"
     CDS             10823..11992
                     /locus_tag="P5516_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HRT91588.1"
                     /translation="MKKTALLLISLLVVFALALSACAPKPPVAEPPVVEEPAVEAPAV
                     EEPAAAVDPMAELVAAAQKEGNLTVIALPRDWCNYGEAIDTFKAKYGIAVNELNPDGG
                     SADEIEAIKANKDNKGPQAPDVIDVGYGFGPQLIEEGIVQPYKVSTWDSIPAESKDPD
                     GYWYGDYYGIMAFEVNSDVVEVPQDWADLLKPELKGQFALSGDPRASAQAQMSVYAAA
                     IYNGGGLDDVQPGLEFFKQVNDAGNFVPLIAKQATVAKGETPVILRWDYNALADKDFL
                     AGNPNIEVVYPASGTIAGIYIQAISAYAPHPNAAKLWMEFLYSDEGQNIWLKGYCHPI
                     RYADMQARGVIPAELSAKLPVFNGPVAFPSIDQINNAKMIIADNWMSVVGADVKE"
     gene            11979..12890
                     /locus_tag="P5516_03775"
     CDS             11979..12890
                     /locus_tag="P5516_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRT91589.1"
                     /translation="MSKNNSFLTDQKRDARKARPSSNSWRKWIGVVPFFIFALLFILL
                     PSVNILAGAFRTNAGRFTLANFAGLFEPSILSAYWVTIKISLTTALVGGLLGFLMAYS
                     VTVGKLPKAVRNMLMTFSGVASNFAGVPLAFAFIATLGRTGLITLILKNVLKLNLYAL
                     GFNLYSFWGLSLTYLFFQFPLMVLIITPALDGLKKEWSEAAENLGATRSLYWRKVALP
                     ILTPSILGAIILLFGNSFGAYATAYSLTGGFINLITIVVGSQIKGDILYNPGLGYALA
                     VGMVIVMAVMMAIYYYLRRKSERWLPR"
     gene            12887..13684
                     /locus_tag="P5516_03780"
     CDS             12887..13684
                     /locus_tag="P5516_03780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRT91590.1"
                     /translation="MKRGSFWSWLWVIIGTLYFLLPLISTFDFSLRAKKGELSFLAYT
                     RVFTDPNFWATMRFSLLIALFTIFLSIVLIAPTAYWIRLRLPKLRGIVEFISMLPYVI
                     PAIVWVFGAIRTFSGTPFFLTNTLFGTYVLLTTGYVVLSLPFVYRSIDVGLAAIDVKS
                     LTEAAQSLGAGWGTILFKVIFPNLKVAILSSSFLTLAIVIGEYTIASFLVGIKAYGPY
                     MSMIGQNKTYESSSLAIISFLLTWAFMGVIQLLTRGKTSGSSMAGAR"
     gene            13727..14776
                     /locus_tag="P5516_03785"
     CDS             13727..14776
                     /locus_tag="P5516_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91591.1"
                     /translation="MAFLTLKNVSKVFGNTTAVEDFNLEVEKGEFVSFLGPSGCGKTT
                     TLRMIAGFELPTSGIITLDGEDITYKSPNQRNVGMVFQSYALFPNLTIGQNIGFGLQV
                     RKMPKEEMEKRIDEMLSIVHLEAHKNKYPHQLSGGQQQRIALARALAINPRVLLLDEP
                     LSALDAKIRVELRLEIRRIQQEMGITTIYVTHDQEEALLISDRIVVMSQGLIEQVGTP
                     DKIYNYPSNEFVAQFVGQLNLLPITDVDLENGKCLLAGQEVRFEHTPGRIISESPRLA
                     IRPEELQIGTGEGRNALKGRVESALFLGAVVRLRVDIGGVFLSADFFNERSRTLPHSG
                     DEVVISFPEYSCWVM"
     gene            14966..16405
                     /locus_tag="P5516_03790"
     CDS             14966..16405
                     /locus_tag="P5516_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase reactivating factor
                     EutA"
                     /protein_id="HRT91592.1"
                     /translation="MAETQTLLSVGIDVGTTTTQVVFSRLGITNAARTGLIPRMAITD
                     KEVLYQSPIVLTPLLDQDLVDAEKLVEIVRREYRAAGIEPAQVGTGAVIITGETARKK
                     NADAILHSLSGLAGDFIVSVAGPQQESLISGRGSGAAQYSREHYQMVTNLDIGGGSAN
                     CAVFKGGNLIGGATMNFGGRILTIDHTSGRVLHIAQAARYILQDCQLNLQTGDTPALA
                     DLRRFTDRMAEVTLELIEGGTSVLARQLYLTPPSQVSGAGSSLMFSGGVGHYYYHPVE
                     IRTLTDVTVHDDVGPLLAESLRNNAALSAYRVITPAETLRATVLGASTQTVSLSGSTI
                     WAEREILPLRNIPVLHLPVEPETQPSALAASLQALVRQNSQDPLEDQYAIALDLNSSL
                     DFAALQHLAAGIAEFAGSLPEQMPLLIIVERDYARVLGQTVKSLAPERPLLVIDQIGL
                     MEGDYIDIGTPLMDGRVVPISVKTLVFYN"
     gene            16465..17832
                     /locus_tag="P5516_03795"
     CDS             16465..17832
                     /locus_tag="P5516_03795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutB"
                     /protein_id="HRT91593.1"
                     /translation="MLLRTKLHGKTYEFPDVRLLMGKANEEKSGDRLAGVAAESAAER
                     IAARLVLAELLLWVLRQNPAVPYEQDEVTRVIQDAVDETVYAEIKDWTIGEFREWLLA
                     DTTTHEMIQRVSAGLTAEMVAAVTKLMSNLDLVLAASKIHVTAHCHNTMGAPGRLSSR
                     LQPNHPTDSPEGIRAEIYEGLAYGCGDSVIGINPSDDSYSSVSRLLEMTYDLIKEWEV
                     PTQNCVLAHVTTQMKCLQSGSPVGLVFQSISGSQKGCESFGINVGMLDEAYELAKKHC
                     FPSGPNFMYFETGQGSALSADAHNGWDQLTLEARNYGLARRYHPFQVNTVVGFIGPEY
                     LYDANQIQRAGLEDLFMGKLSGIPMGVDACYTNHARADQNANENLAVLLTAAGCNYFM
                     AVPMGDDSMLSYQSTSYHDTASLRQLFRLRPAAEFEHWLESIGLMKDGRLTELAGDPT
                     FFLKR"
     gene            17837..18805
                     /gene="eutC"
                     /locus_tag="P5516_03800"
     CDS             17837..18805
                     /gene="eutC"
                     /locus_tag="P5516_03800"
                     /EC_number="4.3.1.7"
                     /inference="COORDINATES: protein motif:HMM:NF003971.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008851 - ethanolamine ammonia-lyase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine ammonia-lyase subunit EutC"
                     /protein_id="HRT91594.1"
                     /translation="MMMEEEKINQIVELVLRQLQEEGLVPGGLKNQPGQPTFPQETIT
                     TGAATVFHPGAALSTHAEIEIDLPDPAGDNLRWLPRVKNAKDPGALIELMASTSARIG
                     VGRAGPRSNTASLLLFQADHAITQDALYRDVDQRLLDEFNLMTVQTRITEGREEYLLR
                     PDLGRQLSDAARQMITEKCQKNPDLQLVVGDGLSALAIESNLRQIFPVIVQGAQSAGL
                     RMGTPFYVKYCRVGVMNDIGDILQPKVLILLIGERPGLGRAESMSAYMAYQPGAGQND
                     ANRDVVCNIFEKGGTNPLEAGAFIVQFAQKMMQFQASGVKLKLAAG"
     gene            18813..19472
                     /gene="eutL"
                     /locus_tag="P5516_03805"
     CDS             18813..19472
                     /gene="eutL"
                     /locus_tag="P5516_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031469 - bacterial microcompartment
                     [Evidence IEA];
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization microcompartment
                     protein EutL"
                     /protein_id="HRT91595.1"
                     /translation="MAVLDKIQAKALSVQLIPRVEDNFARRLNLTPLQLSVGLITSNN
                     DDATYVAVDHATKMADVEVVYAHSFYAGSKHASGLLSGEIIAILAGPNPYEVNEGLKA
                     AVDYLETEAFWYSANEDASVAFFPHVISQTGSYLSRTNNLAVGTPIAYLVAPPIEGLV
                     ALDAALKAAAVSIVALTMPPSETNYMGAILTGEQPACKAAAQMFQEKVLDVVRNPFSG
                     I"
     gene            19503..20093
                     /locus_tag="P5516_03810"
     CDS             19503..20093
                     /locus_tag="P5516_03810"
                     /inference="COORDINATES: protein motif:HMM:NF014886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase alpha/beta domain-containing
                     protein"
                     /protein_id="HRT91596.1"
                     /translation="MELTKPITYFLTTGEVNTTRTFELARARALELGLKTVLVASTSG
                     KTGALAAEFFKGFEVVVVSHSAGFREDNLQELTSDSRQKIEGLGGKVLTATHAFGGIN
                     RAVRRKFNTYQVDEIIAGTLKILGQGLKVAIEIALMAADGGAVRTDKPVLCIAGSNAG
                     ADTAVVLIPAYSQSFFDIRLLEVICLPSPEHREFQK"
     gene            complement(20288..23119)
                     /locus_tag="P5516_03815"
     CDS             complement(20288..23119)
                     /locus_tag="P5516_03815"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HRT91597.1"
                     /translation="MESPAILDLTIGFHALDSSEVEQKLQTKLTCGLTSEEAARRLAH
                     FGKNELTVKKRTSFLQRVLNALNNFVVILLLVAAIISALLGDYIESAVIMAIVILNTI
                     LSVVQESRAEEALAALKKMAAPEASVLRDGHRVNIPAPELVPGDVVFLESGNFIPADL
                     RLVESINMRIDEAALTGESVPVQKNASFRLENNIPLGDRKNTAFMGTTVTYGRGMGIV
                     IGTGMKTQLGMIADMLQSVEEEVTPLQKRLDSLGKVLGWASLGICALVFVVIVVRHLL
                     GPEGSSSLIKTLVDGFMIAVSLAIAAVPEGLPAVVTISLAMGMREMVRRHALIRKLAS
                     VETLGSATVICSDKTGTLTQNEMTVTRIWVDGMDFEITGTGYTPTGDFLYAGEKITVE
                     DYPGLATTLWVGVLNNDAYLETVSDAGGESYHLIGDPTEGSLVVAAAKAGVILGELKG
                     KYPRENEVPFDSTRKRMVTIHSTRGVQFNDASPIHDESKKDWYAIIVKGAPDVVLNLC
                     SKYTQIDDKEILLNEEVRSQILEANDCLTRDALRVLGLAYKLVPNAPKVNDTELDIED
                     LERDLVFVGMMGMIDPARPEVNPALEKARTAGIRTIMITGDYPNTAAAIAKSINLLLP
                     GHTVRTGAELNLLSAEEIREAVKTTDVFARVSPEHKMKIVEALRANNQVVAMTGDGVN
                     DAPAIKRADIGVAMGITGTDVAKETADMVLTDDNYASIVSAIEQGRIIYSNIRKFVYY
                     LISCNIAEILIIFLSTLFTGKSPLTAIQLLWLNLVTDGAPALALANETGDPDIMEQQP
                     RPPNESIINKYMRGGIIVQTIAITSAVLLAYFIGLRAHPELPEFAETMAFTTLSISEL
                     LRAYTSRSEYFPVLKIGLFKNKWMNVGVFISLMLVFLVIYVPFLNTLFNTVPLGWAQW
                     EIILPLILIPSVAAEGKKYLERMLKKRSA"
     gene            complement(23765..26179)
                     /locus_tag="P5516_03820"
     CDS             complement(23765..26179)
                     /locus_tag="P5516_03820"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HRT91598.1"
                     /translation="MNLRAKAREIVAQMTLEEKSSLCSGKDYWYLKPIDRLGLPSIMV
                     TDGPHGVRKQLSSSEDLGISENVPAVCFPTAVSMACSFDPALLREVGQSLAEECLQEG
                     VSVLLGPGMNIKRSPLCGRNFEYYSEDPYLSGHLAAAFIEGVQSKGVGTSLKHFAANN
                     QETRRLTVDAVMDQRTLREIYLSGFEYAIKQARPWTVMCSYNRLFGEFVSQNEYLLTQ
                     ILRQEWGYEGLVISDWGAVVDRVKGVAAGLDLEMPHLDRTHDERVAQAVIAGDLPVAL
                     LNQTAENVTALLLRARGQKRAQYNVSAHRALARRAAAESAVLLKNEGNLLPLNPSSSV
                     ALIGAFAKNPRYQGSGSSRITPIRVNDAVEELKSLGLRFEYAAGYFEDADAPDEGLLA
                     EACQVAAGKDVVLLFVGLPDRFESETFDRETLALPQSHERLIEAVSEVNHNVVVVLYC
                     GGVVEMPWADRVKSILLMHLGGETVNGAVADLLLGRVNPSGKLAETWPFQLEDNPSFN
                     YFPGYPLTVEYREGIFVGYRYYDSAHVPVRFPFGFGLSYTTFSLSDLQLSSNKMGDSD
                     TLKVTCRVSNTGSRAGSEVVQLYIAKKGSVIIRAEQELKGFIKARLSPGESQQVEFTL
                     FQRDFAYYNVNLADWHVEEGEYEVRIGTSSRDIQLKAVLTCTTRVKAALPDLRAQAPG
                     YYHPSARWTVSDADFSTILGRSIPARERVKGAPHTVNSTITDIQDKWFGRLIRHLINQ
                     QVTKIGAKDPYLKMMAEKIVNDMPLRFLTMMGSDSMSILQVEGLVDVLNGHFLKGIKK
                     LRKIAK"
     gene            26716..28215
                     /locus_tag="P5516_03825"
     CDS             26716..28215
                     /locus_tag="P5516_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014051.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HRT91599.1"
                     /translation="MTTNKYIKAVSYNIAYFLVSSLAYLVLTPIAIRVMGGEFYGLWM
                     ILFAVGQLSLIGTLGIETIVNKFAAEVKETHGADANLLSSAILVTLAMAFIVAAILFT
                     FRGFLAQRIGPSPAHLTQFKNALAVYSLMIIPQFTIRVFHGFFLAQIMNRFVRLMDLL
                     NSLLPLLGGVILSLVAQNLTLLALWNLFVSLIVLGVFLFRTYRLIGLRWLPTRVTTRR
                     LLGFSPFMFIESAAINLFQQFDRLLVALMLGPAASGIYSVATGVGLRMSIIAGSVTEV
                     LLPYASRKHSLGEKHKLENIVLKVTRYISLMAALVGGLLIIWMKELLTVWISPQYAEA
                     NALFFCVIILAYSILSLSRPAHQTLVGLGKVRFTSLVYLGSAALMLVTLCLLSMTRGL
                     LGAGYANLVMTLLLCMSLYLFKWFEDDQVWRYFATDLILGMMPLVVALILVGMRCALP
                     LKWVFTIGLLGLVFFVLARDDELKARLKRLVTGSALAKSSERGSQVKDK"
     gene            28283..29491
                     /locus_tag="P5516_03830"
     CDS             28283..29491
                     /locus_tag="P5516_03830"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT91600.1"
                     /translation="MKLVFWGKYIDTYLADTLQRYEESTRMPVQYIVVEGEFTRRKVA
                     ATQAVKNRDQLTVLEKRKRQQQADRVLAANQDAVHLFLSFWGDKRLFGVLLKALHRGR
                     NVAVVFEPYASVPLGYWKDEARILSHFKILSRHIAYRFLWPLIKRAARGPLPCVLAVS
                     PLAQEQLRKVGFPEEVIFPFGYFTERKNGIPVQRKQAEVLRALFSGTLIRRKGLDIAI
                     AAVRRVNSPTVKVQLDIYGAGEVKKFLAHSEPGISYKGVYLQEQAQRVISAYDLMLVP
                     SRHEGWGLVVNEALMQSVPVCASDRVGAKCLLETSGAGLTFRSGDAAHLAALLAHLAA
                     DRHALQVMAQNCNRIANLITPSAGAEYLREVIQFYFAASGRKPKAFWLNASPENPSPD
                     MFSHRGKSGR"
     gene            29613..30674
                     /locus_tag="P5516_03835"
     CDS             29613..30674
                     /locus_tag="P5516_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91601.1"
                     /translation="MTRIAHILGGWSVNIGNAFYQLAGYQVLRECAPGAEITLIPEQP
                     GYPSYWNWKGGNPGNALEYVGRVAPDYLVLMGPVFRREIFNIWADALERLTRAGTRLV
                     LLGVGAMTYDAPHIHLYREFLSRFRPVLMVTRDRETYHLLGDLAEHAYDGIDFAFFLA
                     DYYPTCRIEGFSNTLALNFDKIPEPKIATEGSTSSPGASFHFAGMHWQVKFPRFRTWL
                     ARSSRYGMFLEGCLFGGRNELKVGGFDVIRLDHRPHPLLRAKTFRSPRTLVNDTPYPY
                     LDVYGNAALTLTDRVHAAVAALQYGKPAMLFSSSPRVRLLDRLDLAGIGEQPVWLDME
                     RLREEKTNIRDFLKANLKT"
     gene            30871..31593
                     /locus_tag="P5516_03840"
     CDS             30871..31593
                     /locus_tag="P5516_03840"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF016908.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HRT91602.1"
                     /translation="MNSRISKLIALLRNPFYRRTLFRYRVAAAVEHDALLKRLGKDLR
                     TVVDIGANRGQFALVARFHAPEARIVAFEPLEEPAEVFQEMFRLDALMELHRCAVGAH
                     ASEAVMHLSKADDSSSLLPITALQTRVFPGTQERGTRRVTVRPLTDVLLPRQITPPSL
                     LKIDVQGYELQVLEGGVALLDCFDYLLVECSYAVFYKDQALAGEVLSYLEKVGFVLIQ
                     RCNLIYDQHGVPLQADFFFQRK"
     gene            31629..32852
                     /locus_tag="P5516_03845"
     CDS             31629..32852
                     /locus_tag="P5516_03845"
                     /inference="COORDINATES: protein motif:HMM:NF024968.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT91603.1"
                     /translation="MTNSLGSETLRILLHDYGGYSFTNQLGRTLAGRGHRVLYSYSLT
                     TQLMQRFAPRPAHENLVMAGVQLPRTFNRYSYLRRWRDERLHGRLVGEQVRNFQPEVV
                     LTANTPLDAQVLIQRASREVKAAFLFWMQDLIGLAAQKNLSSRFPLIGPLLGAHYQQL
                     EKKLVIASDAVILISDSFFSYVRGWGITAERMHLLPNWAPLEEIPLLPKENPWAEAQG
                     LADKFVFLYSGVLGLKHDASLFLALAESFRSDPEVRVVIVAEGPFAEKLQAQACVSAL
                     ENLVVLPYQPPAEYPQVLASADVLMTILNTDAGAYSVPSKVNSYLCAGRPQLLSLPCD
                     NDAAQLVSSRKIGLVSEPGDVESWLMNARALYQNRYNHNQMAMYARAYAEENFDIERI
                     TDKCETMMKLALRGG"
     gene            32852..34324
                     /gene="wbaP"
                     /locus_tag="P5516_03850"
     CDS             32852..34324
                     /gene="wbaP"
                     /locus_tag="P5516_03850"
                     /inference="COORDINATES: protein motif:HMM:TIGR03022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate galactose
                     phosphotransferase WbaP"
                     /protein_id="HRT91604.1"
                     /translation="MAGSNGGQALAASAQTRFFDRHKRAFMFAIMLLVDAVALLLAAW
                     LAVLIRSLFSPALTDPALYYALFPYLLVFLLANFFTRLYPGNGITPDEELRGSTYAIS
                     AFMILLAIFLFLTQRGLEFSRFVFLLFWLLAVIFVPLLRLLAKYFGHRLGWWGEPIAI
                     LGFGEKGRTAYHFFKRNSLFGLNPLLIVHSSGNELREESDVRGVATLDEDMLAANPEL
                     VRRLGIRMAVIVPDEVSANLNSLLLDEQPFGLKRLVLISPFTWLGGPVVTAHHLDGLL
                     GLEVEANLLKPLQRAIKRCMDLALGVIGFICSLPLFAVIALLIKLDSPGPVLYRHERV
                     GKGGKPIRIWKFRTMVQGADALLAELVAQNADLRAEWEANHKLKQDPRVTRIGRWLRR
                     TSLDELPQVINILKGEMSLIGPRPIVREEVKFYQEGFALYQKVRPGLSGLWQVSGRSD
                     TSYAYRVSLDEYYIRHWTIWLDIFILLKTLWVVASRTGGY"
     gene            complement(34404..34850)
                     /locus_tag="P5516_03855"
     CDS             complement(34404..34850)
                     /locus_tag="P5516_03855"
                     /inference="COORDINATES: protein motif:HMM:NF016763.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HRT91605.1"
                     /translation="MLFWAAVIFILSADSNPYSHIPNRLYSWLWWSKLFGRSLIFYLG
                     GISHILEYAILSFLFARALWQRERPTRGQFIKAISLVLFYAFTDELHQYFIPGRAFQL
                     VDLALDTLGALLGLGAYWLYLDRLEGKQSRLTYALQGILTSCKTGK"
     gene            complement(34901..>35418)
                     /locus_tag="P5516_03860"
     CDS             complement(34901..>35418)
                     /locus_tag="P5516_03860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91606.1"
                     /translation="NITDRQILEERLDINKMRCECIAMLRREFPQQFFGGIYPSDYAR
                     TVHGNYVIPDRRVFHKKAYLALMHNSDIGIATMGLFGSNGGKLGEYVAASKAIVSERL
                     RYQVPGDFSSGLNYLSFATPEECVAQVRRLVDDFDLRLAMMQRNHQYYSSYLRPDVLV
                     WNSLQKAMEVQ"
     assembly_gap    35419..35518
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<35519..36524)
                     /locus_tag="P5516_03865"
     CDS             complement(<35519..36524)
                     /locus_tag="P5516_03865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91607.1"
                     /translation="MSAAENKKVTLILPIRDEGPTIRAALDAVLAQNYPAGQMEILIA
                     DGMSTDGTREIVREYQHKHANIKLLDNPGRIVPTGMNTALRQAAGDIIIRVDGHCVIA
                     PDYVENCLRHLANDTVDGVGGPMKTIGLTPLAETIALAMSARFGVGNSAFRTTQGQTK
                     YVDTIPFPAYKREVIQKAGLYDEELVRNQDDEYNYRIRELGGKLLLADDVRSVYYSRG
                     SMRGLWKQYYQYGFYKVRVLQKHPQQMSARQFIPLIFILSIFGSLALALFRNEGWVLL
                     ASILGAYLFAAILASFRISIKKGWKNFFLLPIAFITMHFSYGLGFAVGLIAFWKRWGD
                     K"
     gene            complement(36528..37928)
                     /locus_tag="P5516_03870"
     CDS             complement(36528..37928)
                     /locus_tag="P5516_03870"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRT91608.1"
                     /translation="MIKQRVYQVLPSPLKNLAASVWGYYLRHLRFDHQTETLVEEALQ
                     RESWDRNQWEKWQQVQLARLLQVAATTVPYYREQWQRRRRNGDKASWELLQNWPILDK
                     KEVRDNPRAFLSENSKSKHLYIDHTGGTTGKPTLIFESREVIRNIYALHEARTRRWFG
                     VHHRDRWGMLGGQKVIPLHQRRPPYWVWNGGLNQVYFSVFHINPETAKDYVRALWKYR
                     PVHLMVYPSSCAILSKFILEQRLRPPQIKVIVCNSENTLPKYRELMTRAFNCPVIDTY
                     GMAEACAAASECLALTQHYWPESGIIEVFDPQKREFTTEPGTAGEYVLTGLMNDDMPL
                     VRYANHDLGYLPNWQHQCACERKLPSLASIAGRSNDLVLTKDGRELYILDSLYNGLPV
                     IEGQLIQHTLDSFEVKVVPDETYSRSAMLGAITARLQQYLGAVNITLTEVTEIEKNAN
                     GKFKPFVSMINAQTYR"
     gene            complement(37925..>38296)
                     /locus_tag="P5516_03875"
     CDS             complement(37925..>38296)
                     /locus_tag="P5516_03875"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT91609.1"
                     /translation="SNITITGFISRSQLQEWFTKPDIYLNTTNCESFGIAVVEAAACG
                     LCIVTTNVGELPYIWEDGVDALLVPPNDPQAMAAAVKRILTDPELASRLSANARKKAE
                     TYDWSAILTRWEALFNKLLKS"
BASE COUNT         8838 a         9774 c        10389 g         9195 t
ORIGIN      
        1 aaccgttcag ggcacgccga ccttcagttg gaaagcggtg cctggggcga agtactacga
       61 attatgggtt gatgaagcgg atggcgattt ctcagacccg gtctataaaa aatcaatgct
      121 gacgacgttg aaccacaaac ctccgatcat catggaaggg ggatatcgct ggcgggtgat
      181 tgccagtgac ctggcgggca atgaatcaga ggggggcgag ggctggattg ttacggtcaa
      241 cccgttgaag cctgtggcgc cggtgctgac cgggcctgcc aacggcgtat ttctcaatga
      301 tgagaaaacg gagatcgtgc tgagctggaa cccggtgacg gcttacgcgc agcgctacga
      361 attcagggtg gacgacgacc cgcgctttac ctcccctgca tattcggaat cgtcgggaaa
      421 tgaaacttcg tatctgctga ctgacactgc cgcgcatttg ttggataata ccaggtacta
      481 ctggcaggtg cgggcggtga actcggctgg ggcggagaca ggggctggag cgtggagcgc
      541 ggttcgctcc ttcacggtgg accgtacgcc gccggttaat gccccggggt tgagaacacc
      601 cgcgaatgaa gcatctatgg cgacctatac ccccgcgctt tcggtaaatt cgctgaaaga
      661 cgcgaaatgg taccgcttta ccgtgtacca gtcaggcacc tgcgaggcga acgacggatt
      721 catcagcccg gatgtgaccg ccaccagctg ggcagtaccg gctgcccagg cgctggctta
      781 tggtaagtat cactggtgcg tgcaggcggt cgacgcggcg ggcaacgcat cctcgtggtc
      841 ggaggcgcgc gtgttcaccc tcacgttcca gaaccagccc gggtttgcgg cgacaacggc
      901 gagcgctacc cctgggttct catggtacgc ggtgagaggc gccaggggct atcggctgga
      961 agtgaaacca gttgtggagg agggcaacac cgccacgcgt gaatactccg tatacctggg
     1021 cacggtaacc agccacgtta tccccgccat caaagcgctg gacccggggg cgtatcagtg
     1081 gcggctgcgg gtgtacaccg cggcgggcag cgaggaaacc ccctggaggc tgctggtggt
     1141 gctgcctccc gcgccggtat tggacacccc ggacaatggt tccgttcatc ccggcgtaac
     1201 ggtagagctt aactggttcc cggcagcggg agcggcacgc tatgaaattc aggcggataa
     1261 taacaacaat tttagaacgc ccgaaatcga ttatgaattc acagaaggtc cggttacgac
     1321 caaagaggta acttttcctt tcgctggcgg gttttactgg cgcgtgaggg cgtataacta
     1381 cctgggcaac ccgggcgcgt ggagcgcggc gaggtattta cgactcacaa caccctgaag
     1441 gcaaaacgct aattaaccag cgtcagatgt gatgatacga gagggtccca gcgcggtttt
     1501 ccggcgggac cctttgctta tccttgaact gtttggggta ataaacccgt ctgcgttaaa
     1561 aggcctgcgg caatgagcgg tttaaggacc aactgttccc caggcgctgg ttttggtggg
     1621 agagggagtg ggcgtgatgc tgcctgtggg tgtggctgtg cccggggtgg tggtgaagga
     1681 gggcgttttg gtgagggtgg ctgtcgctct cacgatggta ggcgtgatgg ttgggcttgg
     1741 cagcgaagta gaatagacca tcacgggatg tgtgtctcca ccggagatgt ccattacata
     1801 attcactgcc gcgcggcacc cgccttcaag gttggcggcc acgtgcagcc aatccagccg
     1861 gttgcccgct tcccaggtgc gcttcaaagc gtgccacagg cagccaggac cagcgttgta
     1921 attgaccagc gtgtagcgcc acaggtcatc gtgagaggct acagcgccgg ctgaagcccc
     1981 ggtgaggtta tacagcatgc ggtccacctg ggcgcaattg cccacgatcg tttcgccaaa
     2041 tatttttacg ctgaaattgg cttgggtcag gtcgattccc agatcgcaat ccgggcaggt
     2101 ggcattggtt ttttgtacca gcgccccccg cagcgttgtc tgctgttcct ggctgaggtt
     2161 accataaccc cgggcgcagg tatcttcgta aagaacgaga gggcagaact gagtataaaa
     2221 ttcagggttc cacagcaacg cggcttccgc cccgtcttct gtgagctgac caaaccccac
     2281 ctcttccgcg ttatggtaga tgccgggcca aagctgactt tcgtgggcaa aaatattttt
     2341 taacaggatg gaaggaatgc cggtctcgat tgacgtacgg atgatctcat catcaaactg
     2401 gttttgccac aaggttaccg cttgcatggc ggcttccatg ccgcactggt tggcggaagc
     2461 gtagtaatcc aacccgccgt acgggcaatc gccagcgtcg accacgccat tctgaatcag
     2521 catggcggcc aggtaatgca gcggcagcga agtggacaaa ctagcacgat cagccgggtt
     2581 tttcagccag gcaggaagcc cctccacaga ggggaaggac tgccatatcg ccgcgcagga
     2641 ggacatttgc tgcgcctgcc attgagagct gatgatatcg acgtaatacc ctgagcgctc
     2701 cggcgcgcca gcgttgtcgg ctggcatatc cgcccacggc agcactcgaa tataggcggt
     2761 gtactcctct gtggtgtcgc cataactgga ttctccccag aaggtcaaca tcactccctg
     2821 ctcgcctgtt tgccatagcg gcagcggaca gcggctgcct gcgcaagaga atttggttcc
     2881 cccgtaaacg ccatgaatgc gggtgattga ttcgttggcg agtttctctt cgccgatgag
     2941 cacgacctcc ggtcgccctt cccacttgtt ggttgtgccg ggcaagacag agccatttac
     3001 cgtgatccac acgacgggag gaggcaggtt gaccatgatc tcgtttgcgg agagcgaaga
     3061 acgcgtattt tgcaggtaca aaccgctgca tgaactgagg tcctttccct cttctccggc
     3121 agtgcagggc ggggtggtga gccaggtgtt taacacctgg tcaccgcaga acttttgtat
     3181 ctccagttcc gagggggtgc cagcgtgagc agtcgtgaac tggcagacga tggtgttgtc
     3241 actccagcga gccagccacc aatcgtattg gctgtcgtcc accgttatcc aggtaacccg
     3301 gtcgggtcca tcctgagctg aggcagggtg aatattcacc gagttggtaa tcaacaatgc
     3361 caccgtcagc accgtcaggc aaacgagcaa tatcccttta ccggttaaat atgaaaacgc
     3421 ccctcggaca cgcatgatga ttcaaatttt tccttgatga aaaggatagc atagatcggt
     3481 gcttaagtaa atgaatttac aatctcgcaa aatcgcataa tgaacttata atcatagtaa
     3541 aagatttaca aatttctaag attgagagta aaaatgcaag agagagccca ggggggtctt
     3601 ttggaacccc aaacctattc tatctcgggt agtgatttgg tggcgttaat ggaggcggta
     3661 ctggcaaaaa atggaacttt tcgcttcacc gccagcggca gcagcatggc gccgttcatt
     3721 caaacaggcg acatggtcac aatctcctca ctgcataagg gaagggtagg attgggcacg
     3781 gttgtggctt tcaaacaccc tgtgggcggg cacctcgtga ttcaccgcgt tgtgggcgtg
     3841 gcgcatggca cttttttgat aaagggcgat aatacacccc atcgatctga tggctggatc
     3901 ccgtcaggct gtttgatcgg ccgcgtctta gaaatcacca ggaatggcag gcgcgtatgg
     3961 ctcgggttgg gtgttgaaag gtatttaatc gccttttttt cgcgctggca atggttgatc
     4021 ccgctgagag gcgcgctcgc cgcgctgcgc cgcaggtaga accccgaacc atgggcctga
     4081 taccgagatc gttcgctggt tatcttcaag gttgagggaa gcaggcaaga gagtgtggtc
     4141 catcatcatt aagaaagtga attatactta actaccaata gcagaaaggg tggaagacct
     4201 tcatggataa tgaacttgaa gaacgcagca gcctcgaaag cattttagat tacgcgcaaa
     4261 ccttgtggcg ctgggcttgg ctgcttctga tcgtggcaat cgccgccggc gcggttgcgt
     4321 tttaccttac caatcaaaaa ccacgcgtat atgaatcttc aaccagagcg gtcgtcaatg
     4381 tcgtcaccgg ttcgaatttt tacgacgcct attcggcttc gtttggcgct caacgcctgg
     4441 ctgagacgta ttcacagacg atgatcacac ctgagctgct gcaatcagtg tcagaacggc
     4501 ttggttttga agtgaccggc aaaatcaccg tgacccccgt ggagaattcc ccgattttta
     4561 ccattgttgt cacagacaac gacccgcaaa aagcagcgga caccgccaat atgctgatta
     4621 ccatcttcgc cgagaaggtc atgaaggatc aatcttcccg ctattccgag ctgaaacagg
     4681 gcctggaaga ggaaattgcc cgcattgacc aaaatctgac tgatataaat gagagactgg
     4741 ctattttgca gatcaaagag gctgaactgg cagaggctga aaaacgcgcc gcccagatgg
     4801 ctgctgaagg gcttgaacct attgaggttg tgcgcgaccc gaatgattat gtgacccgtg
     4861 cacaactgga actggcgcaa tcccaatatc agtcaactcg ttcatcacta ttctacaatt
     4921 tacaacaaat taaattaacc gaggttcaat ctgccactac catcagtcag atcgacaaag
     4981 cgatggctaa tccagaaccc atacagccgc aaccgtttaa gagcgccggt ttggcggcga
     5041 tcgtggggtt gatggtagcc gcgggaattg ttttcttgat cgcttttttg caggatgaaa
     5101 tccgggaccc ggaagagttt gcgcgccgat ggggggtgcc ggtggtgggt gtgattaccc
     5161 actacccgtc caaccaggac aagttgatca cggttgccaa accgcgtatg tcggtgtcag
     5221 aagcctttcg ttcactgcgc accaaccttc aattcagcgg catcgacacc cctttgaaat
     5281 cggtgctggt aacaagccca tcgccgtcag aaggaaaatc ttcggtcgtg gccaacctgg
     5341 cgacggtgat tgcccagagc gagaaggaag ttgtgttagt ggatggagat ttacgccgac
     5401 ccacgctgca caaggtgttc aaattatcca accgcatcgg tctttcggat tactttatcc
     5461 gcgaacctga aaaaatgtct ggcgtggtaa agaaaacgaa agtgaatggg ctgaacataa
     5521 tctcatccgg cagcctcccg cccaaccctt ccgagctttt gggttcaacc aaaatgcacg
     5581 atgtattagg tttgctggag aagcattttg atatggtgct gctcgactct cctccgctgc
     5641 tggcggtaac agacgcgctg atcctggcga aaagtgtgga tggcgttctg ctggtggttg
     5701 acccgaaaaa gaccaagcgc ggggccatcc gccaggccat tgaacagctg cagcgcctgg
     5761 acgccaggtt gttaggggtg gttctgaaca atatcaaaat aaaacgatcg agttattatt
     5821 acaaccgcga ttactactat agcaaacagt acggcaagtc taacgaggac ggtactcacg
     5881 aagaaccagc ggtcgaagag gataaaacgc ggtagagggc ccaatcacaa atgaccggcg
     5941 agccgaaccc agcggtgaaa gggacgaaaa cggacaaaaa gagaaacgac acactctttt
     6001 gtcctttcct cggttttgag gatgatccga caaccgcgct ggcgtacccc gccaagctca
     6061 atttttgttt tcgcgctaaa cccgccgccc cggtcagcct ggcgcaccaa cgcgataaat
     6121 gcctgaccga gttctatgtg gattgccccg tttttcaaag tgaaaacccg atcccgctgc
     6181 cagagagcat ccggggtgaa cggaaatcga aaacttttca gttcaactgg gtgcccctag
     6241 ttgctttaat agcagtagtc atcatcggtc tcgtagctct gctgctcacc ggggtgttta
     6301 cttttcgtgg attcacaccg ccgctggctg acatttcgac cgcgactcac acgcctgcac
     6361 acctaccagt ggtgccgctt tctgcagaac cgaccactac tgttgaagca actgccacac
     6421 tggtgccaac agtgaccgct acctggttga tcccgggtaa gcacacaccg cgcgcgttgg
     6481 aaacgccgtt cggcacttcc cctcaattgg tgatccacaa ggtcgccgcg ggagaagggt
     6541 ttattttact ggcagaaaaa tttcatacta ccgctgacgc gatcaaggcc atcaattacc
     6601 aacttccgga ttcattatgg gagaataccc tgctggtgat accggtcaac accgatgatg
     6661 ttgcgggatt gccagctttc agcgtggttg agatacgcgc ggaagggatg acgattgaat
     6721 cttatgccgc ccggatgcag ctggatgtgg aaatgttgaa aaagtacaac gatctgcctg
     6781 aaggttattc gtttcaaatt ggcgaaatgc tgatcgtacc gatgacagaa tgatcaacat
     6841 gggtagagag tgaaccatgc cgaatgtcct ttttgtatgc agcgccaacc ggttccgttc
     6901 tgtactggca gctgaatatt ttacatctct gcttgaaaaa caagcaccga gcggcgaatg
     6961 ggttgtgggc agcgcgggta tttgggcaag agaaggcgtg gctcccacga aagaagcgct
     7021 tcaatgggcg gcgggtaaac agcttcatat tgagtctgtg cgatccagag agatatcaaa
     7081 cgcgctcatt gtcgaggcgg acttgatcat cgtgatgacc aacggtcagc gggaagccat
     7141 ttctatcgag tttcccgagg cgagagaaaa actcaccctg ctctctgaaa tagccgccgc
     7201 tcaaatctat gatattccag acccaatcat agaaagcggt gaagaaccgc tacagatcgc
     7261 ggaagaaatt tccacgctga ttgacgccgg gtttgagcga attctcaagc gggcggctgg
     7321 gcgagccaat aacctgggag cgcagcttcg tggagcataa accgcctaac gcgaaaagac
     7381 gcctgctgtg gctgttattg gtattattgt tcctggtaag ctgcggatat ttgctctgga
     7441 caaaccccct tctgggcaag cggcttccgc aattcaccta tgagccgttg gccacattta
     7501 ccgccatccc caccagcgcc gcgcttactc tcacacctga aacggttaaa acagaacccg
     7561 cgctggcgac tgttgagcct gaacccacaa gcgtttcacc tgtttgtggg caaacccagc
     7621 caatgataat cctggctttg ggcattgatg aatcagaaca ggcggatatg atcagggttg
     7681 tgagagtgga ttggctgaag caccaggttg tggtgttatc catcccacgc gatttctggg
     7741 ttccaatacc ggggctggag gagcataata tcagccagtt tcgcattaac gcggcttatg
     7801 gctacggcga gtacttcaac gggcgtggtc agggcgtggt gaagttttct gaaaccatgt
     7861 accagaacta tggcgttaca tttgaccggt atgtggtggc gcattttaat acttttacag
     7921 aactggttga ccaaataggg ggagttgaca tcgaccttga agaacccatt ggggcgtatg
     7981 cgagcgcggg tcatcatcac tttgatggca aatcagccct gcttttcgtc aggcaaagga
     8041 tagcggatct agacgcgtat cgtatccagc ggcagtcagc cattttaaag ggattgtacg
     8101 aaaaactttc taaacctgaa tatttagtcc aactccctgg gctgggagtg aaatttatta
     8161 aggataagtc ggtcatcact gacttacgct tgcaggatgt atatacattc aattgtttca
     8221 tccgtgaaat caaccgtgat tctttggttt ttattgatat tccttatgac ctgtacaccc
     8281 ctatgatcac caattatggc cgacatatca aactgcccaa gccagaggct ggggtcttta
     8341 tccaaaatgt gatcaaaaac ggaactgaat aaccttgccg gtacctcccg gaggcaatag
     8401 gcggaaaagt ttttcccgga cagattaagt caaaatacat gcaaacactt cttaccggct
     8461 ggcaattttc ttgttctctg cccaggtggc tccaaggtct aactcagacg acgcgtaaga
     8521 gaaaaacctc aaagaaatgc ttctgctgca gcccgaccat ttaccaggaa aagaaacgca
     8581 aaacgctact gggtagcgca cggcggcgct cttttcggta tccttatctt tctataacag
     8641 gagtaataag gtataatata tcttaatata ttgataagaa tacagcgatc aaggaaatga
     8701 aaccatgagc gaactgatca acaataagca gaaacgagtt gagttaatga agtctcttat
     8761 ccggcaactt caccagggca tggcagaaga ccgtgtacgc atgcagctgg aaaccctgct
     8821 ggacgaagcc gattacaacg atgtgtttgt aatggaggta caactgattc aagagggtat
     8881 accggcggag gttgttcagg atttgtgtga tacgcatacc cgtgtattga aaaaacacct
     8941 ggacctgcaa gaaacgccgc aaaccatacc tgggcaccct gtacacacct ttgttgaaga
     9001 aaaccgtgaa ttgaccaaag tgaccgcgca gatcaggcac ctgatgagcc tgatagccga
     9061 aaagcccgct gaagaagacg tgagcgagca aatgggcgaa atccaccagt tgctcaataa
     9121 cctgatggac gtggaaaaac attatcaacg caaagaaaac ctgctcttcc cgtattttga
     9181 aaaaaacaac cttcccggcc ccccagcggt gatgtggggg aagcatgacg aagtcaggga
     9241 attgttaaaa accacacttg aggggtttca acaggtgaag tccttttcag cgggagaggc
     9301 cgcggctttt aaccagttca gtgtgagcat ggtggtggac gcgattgatg agatgatata
     9361 caaagaggaa aagatcctcc tgccgaccgc ccttgatatt ttaagtgaag aggattggta
     9421 cgccatctac ctggaaagcg acgagggtgg ttattgcctg tacgttccgc aattcacctg
     9481 gacgccagaa ggtgatttta aaagggaaat aagaaatcca tctggagtgg gcgctcgaat
     9541 acaaatgcca accggcagct tcactttgga tgaactggtt gccttgttct ccactttgcc
     9601 ttttgacctc acttttgtgg acaaggacga tagcgtgcga tatttcagcc ctggaacaga
     9661 aaggattttt aagcgtacgc gcgctatcct gggtcgcaaa gtccagtatt gccacccgcc
     9721 gaaaagcgtg catatcgtca accgaattgt gaaggacttc aaagaaggga aacaggacca
     9781 ggcgcgcttc tggatcaata agggcgggcg catggtctat attgtctatt atgcggtgcg
     9841 cgatgagcaa ggaggttacc ttggcacatt ggaggtaacg caggacgtta cggatattcg
     9901 cgcattagaa ggcgaacgcc gcctgctgga atatgatgga gcaccgaaat agggaagaga
     9961 cgaaaatcag gagaaagaca tggaaataaa cgcgaagacc aaactgcata acctattgag
    10021 cacttatcct caattagaag aaaaaatcat ccaggctgcg cctgcattta agaacctgaa
    10081 gaaccccgtc ttacggcgca cggttggaca attggcgacg gtggaaaaag tggtgcaaat
    10141 tggcgggctt gacctgacga cttttatcaa ccttctccgc cgtgaagtag gacaacctga
    10201 attgatcccg gatccttcct cggataatgg aaaagacaaa agcgcgccag gcgctaaacc
    10261 agattggact aacggtaaga ttcagttcac tgtggatggg aaagaactgc tcgcaaaagg
    10321 tgaagtgccg gtaaaccacg tgaatgcgct gctgccacag ctggaagaaa acaagctcat
    10381 cctcctggtg acagattttg aacccatccc aatgatcgag gtgttgaaaa agcaaaaacg
    10441 ggaagtgttt cacatggtgg attcgcacga cgcgggaaaa cacctgacgt atataaaata
    10501 aatatggaga cgcttcagcc catccgctgt atttgagata acacattgaa acaataggcc
    10561 ggtaaacggc ctgttgtttt ttttatacct cactggggca tccaaccaag cattccctca
    10621 aaccatgaaa ttgaaatcca tcttcttaag gttattgggt tggcaaaact tgattgtaat
    10681 tttcttgtgt tactatataa tatgtctatc ttcatcatta agccgtttca caagaaggga
    10741 ggtttccggt cattgtctct gtaacacaac ctgttttgaa gaaagcaaat gttacaaaaa
    10801 acaaaaacct gaggagagaa acatgaaaaa aaccgcatta ctcttgatta gcctgctggt
    10861 ggtctttgcg ctggcattat cagcctgcgc gccaaaacca cctgtcgcag agccgcctgt
    10921 tgttgaagaa cctgctgtcg aagcacccgc cgttgaagaa cctgcagcgg ctgtagaccc
    10981 catggctgag ttagtcgcag cggctcagaa agaaggcaat ctgaccgtga tcgccctccc
    11041 tcgtgattgg tgcaactacg gcgaggctat tgacacgttt aaagccaagt atggaatcgc
    11101 ggtcaatgag ctgaaccctg acggcggttc tgctgatgaa atcgaagcca ttaaagccaa
    11161 caaggataat aaaggcccgc aagcccctga tgtgattgac gttggatacg gctttggccc
    11221 gcagttgatc gaagaaggga ttgtgcagcc ttacaaagtc tccacctggg attccattcc
    11281 ggctgaatcc aaagatcccg atggctactg gtatggtgac tattacggca ttatggcctt
    11341 cgaagtcaat tcagatgtcg tcgaagttcc gcaggattgg gctgacctgt taaaaccgga
    11401 actaaagggt cagttcgccc tctctggaga tccgcgtgct tcagcacagg ctcagatgag
    11461 cgtctacgcc gcggctatat acaatggcgg cgggctggat gacgtgcagc cagggttgga
    11521 attctttaag caggtgaacg atgcgggtaa tttcgttcct ctgattgcca aacaggcgac
    11581 tgttgccaag ggcgaaaccc cggttatctt gcgctgggac tacaacgctt tggcggataa
    11641 ggacttcctg gctggcaacc cgaacattga agttgtctac ccggcgagcg gcacaatcgc
    11701 gggtatctat atccaggcga tctcggctta cgctccgcac cccaacgctg ccaagctttg
    11761 gatggaattc ctgtatagcg acgagggaca gaacatctgg ctgaaaggct actgccatcc
    11821 tattcgttac gcggatatgc aggctcgcgg cgtgatcccg gctgagttat ctgcgaaact
    11881 ccccgtcttc aatggtccag ttgcgttccc gagcattgac cagatcaaca atgcgaaaat
    11941 gatcattgca gacaattgga tgtccgtcgt cggcgctgat gtcaaagaat aactctttct
    12001 taactgatca gaaaagggac gcgcgaaaag cgcgtccctc cagcaatagt tggcgtaaat
    12061 ggatcggggt tgtccctttc tttatttttg cgctcctgtt catcctgctc ccttccgtga
    12121 acattctggc tggcgccttc cgtaccaatg ccgggaggtt caccctggcc aacttcgccg
    12181 ggctctttga accatccatt ttgagcgcct attgggtgac gattaagatt agcctgacta
    12241 ccgcgctcgt tggtggcttg ctgggtttct tgatggccta ttctgtcact gtgggtaaac
    12301 tgcccaaagc ggttcgtaac atgctcatga ccttctcagg cgttgcctcc aactttgctg
    12361 gtgtccctct ggcgtttgcc tttattgcca ctttagggcg aacgggtctt atcaccctca
    12421 tcctcaaaaa tgtgctgaaa ctgaacctgt atgcgctcgg gtttaacctt tacagtttct
    12481 ggggactgtc gctcacgtat ttgttttttc aatttcccct aatggtcctc attatcaccc
    12541 cggcactgga tgggttgaaa aaagaatgga gcgaggcagc cgaaaacctt ggcgctaccc
    12601 ggagccttta ctggcgcaag gtcgctctac cgatcctcac tccttccatt ttaggcgcga
    12661 tcattctttt atttggtaat tcttttggcg cttacgctac cgcttactct ctcaccggag
    12721 gtttcatcaa cttgatcacc attgtggttg gctcacagat caagggtgat attctctaca
    12781 acccgggcct gggttatgcg cttgcagttg gcatggtgat tgtgatggcg gttatgatgg
    12841 cgatttatta ttacctgcgc cgcaagagcg aaaggtggtt gccaagatga aacgcggatc
    12901 tttttggtct tggttgtggg tcattattgg aactctttat tttcttctcc ctctcatttc
    12961 aacatttgat ttttcgctac gcgctaaaaa aggagaatta tcttttctcg cttatacgcg
    13021 cgtttttaca gaccccaatt tttgggcaac catgcgtttt tccctgctga ttgccttgtt
    13081 taccatcttt ctgagcatcg tccttattgc tcccactgcc tactggattc gtttgcgatt
    13141 gccgaaattg aggggaatcg ttgagtttat ttctatgctg ccttatgtga tcccagcaat
    13201 cgtgtgggtt tttggcgcca ttcgaacttt cagcggtact ccttttttct tgacgaatac
    13261 cctttttggc acctacgttt tacttactac cggctatgtc gttttgtcat tgccgtttgt
    13321 ttatcggtca attgatgttg gactggcggc gatagacgtg aaatcgctta ctgaagccgc
    13381 gcagagcctt ggggctggct ggggaaccat tctgttcaag gtcattttcc ccaacttgaa
    13441 ggtcgccatc cttagcagct cttttctgac gcttgcaatt gtcattggcg aatacaccat
    13501 tgccagcttc ctggttggga ttaaagccta cggcccgtac atgtcaatga tcgggcagaa
    13561 taaaacctat gaatcttctt ccctggccat catcagcttc ttgctcacct gggcctttat
    13621 gggggtgatt cagctactca ctcgcggaaa aacttctggc agctcaatgg caggtgccag
    13681 gtgatatata gcaaaaacaa taaaccttac ggaaaaatgg tgacttatgg catttcttac
    13741 tcttaaaaac gtgtcgaaag tttttggtaa cacgacagcg gtggaagact ttaaccttga
    13801 ggttgaaaaa ggcgagttcg tttcatttct cggaccttca ggatgtggaa agaccactac
    13861 gctgcgaatg attgcgggtt ttgagttacc aacaagtggg attatcacac ttgatgggga
    13921 agatattacc tataagtcgc cgaatcaacg caatgtgggc atggttttcc aatcctatgc
    13981 attgtttccc aatctgacca ttgggcagaa tatcggtttt ggtttacagg tgcgcaaaat
    14041 gcccaaggaa gagatggaaa aacgcatcga tgaaatgtta tccattgttc atcttgaggc
    14101 ccataaaaac aagtacccgc atcaactctc gggcgggcag cagcaacgca tcgcattagc
    14161 cagggccctg gcgatcaacc cacgcgtact gttactggac gagccacttt ccgcgcttga
    14221 cgcgaagatc agggtggagt taaggctgga aattcgccgt attcaacagg aaatgggtat
    14281 taccacaatt tatgtgaccc acgaccagga agaagccttg cttatttctg atcggattgt
    14341 ggtcatgtct caagggctta ttgagcaggt gggcacgccg gataaaatct acaattaccc
    14401 ctctaatgaa tttgtcgcgc agtttgtggg tcagctgaac ctcctgccaa tcacggatgt
    14461 tgatttagaa aatggaaagt gcctgctcgc cggtcaggaa gtgaggtttg aacacactcc
    14521 cgggcgcatt atcagcgaat ctcccaggtt ggcgattcgc cccgaggaac tacaaatagg
    14581 aaccggcgag ggaaggaatg ccctgaaagg acgggtggaa tcggccctct ttttaggcgc
    14641 cgtggtaagg ttgcgtgttg atatcggcgg cgtctttctc tctgctgact tcttcaatga
    14701 acggtctcgc actttacccc actccggtga tgaagtggtc atcagcttcc ctgagtactc
    14761 atgctgggtg atgtaaagag ttactgaaag cacataaaag agagaaacag ccttttatcc
    14821 cagccgggat ggcaaaatga actcaaattt attgagaaat gatctggcga gattgggcga
    14881 tcatcacacg ataaacccgc ccgaggttgg aaatttctga gttgataaaa ataatgaata
    14941 tttccatttg gttagaataa accctatggc agaaacccag accctgctct cggttggcat
    15001 tgatgttggc acgaccacta cacaggtggt tttttcgcgc ttagggatta cgaacgcagc
    15061 ccgcacgggt ttgataccgc gcatggccat tacagataaa gaagtgctgt accagagccc
    15121 gattgtgctg acgccgctgc tggaccagga tttggtggac gcggagaagc tggtcgaaat
    15181 cgtgcgtcgt gaatatcgag cagccgggat tgaacccgcg caggtaggca ccggggcggt
    15241 gatcatcacc ggagaaacag ccagaaagaa aaacgctgac gccatcctcc attcactttc
    15301 ggggctggcg ggcgatttca ttgttagtgt tgccggccca cagcaagaaa gcctgatttc
    15361 tggccgagga tcaggagcag cgcagtactc cagggagcac tatcagatgg ttaccaacct
    15421 ggatatcggt ggagggagcg cgaattgcgc ggttttcaag ggaggaaacc tgataggcgg
    15481 cgcgacgatg aactttggtg gaagaatcct caccatcgac cataccagcg ggcgcgtgtt
    15541 gcacattgcc caggctgcca ggtatatttt gcaagattgc cagttgaacc tgcaaacagg
    15601 agacactcct gcattggctg acttgcggcg ctttacagac cgtatggcgg aggttacgct
    15661 cgaactgatc gaaggcggca cctctgtttt agcccggcag ttatacctca cccctccttc
    15721 acaagtttca ggcgcaggat cgagcctgat gttcagcggc ggggtcgggc attactatta
    15781 ccatcctgtg gaaataagaa ccctgacaga cgtgaccgtg cacgacgatg tgggccccct
    15841 gctggctgaa agcttgagaa acaacgctgc tctttcggca taccgggtta tcacgccggc
    15901 tgaaacgctg cgcgccaccg tgctgggggc gagcacgcaa accgtctcgc tttctggttc
    15961 caccatttgg gctgagcgtg aaatccttcc gcttcggaat atcccggtgc tgcatctgcc
    16021 cgtagagcct gagacccagc cctccgcgct ggccgcaagc ttgcaggccc tggtgcggca
    16081 gaacagccag gacccattag aagaccagta cgctattgcg ctggacctga actccagcct
    16141 ggattttgct gcgctgcaac acctggcagc cggaattgct gagtttgctg gcagcttacc
    16201 agagcagatg ccgttgctca tcattgtaga acgggattat gcccgggtac tggggcaaac
    16261 ggtgaaaagc ctggcgccag agcggccgct gctggtgatc gatcagatcg gtttgatgga
    16321 aggcgattat atcgatatcg ggacgccatt gatggatggg cgagtggtac ctatcagtgt
    16381 gaaaacgctc gttttttaca attagaccaa tctgttgatt cattttataa tcaaggtacc
    16441 agtacctcaa taagcggagc gcggatgctg ttaagaacaa aattgcacgg aaagacctat
    16501 gaattccccg acgtgcggct cttaatgggc aaagccaacg aggagaaatc aggcgatcgg
    16561 ctggctggcg tggcggctga gagcgcggct gaacgtatcg ccgcccgcct ggtactggct
    16621 gaactgctat tatgggtgtt acgtcagaac ccggcggttc cgtatgaaca ggacgaggtt
    16681 acccgtgtga tccaggacgc ggtggatgag acggtttacg cggagatcaa agactggacc
    16741 attggcgagt tcagagagtg gttgctggcg gatacgacca cgcatgaaat gattcagcgc
    16801 gtctctgcag gcctgaccgc cgaaatggtg gcagcggtca ccaaattgat gtctaacctg
    16861 gacctggttc tggcagccag caagatacac gtcactgcgc attgccacaa tacgatgggc
    16921 gcgccaggga gactttccag ccggttacag cctaaccatc cgactgactc gcccgagggc
    16981 atccgcgctg aaatctatga ggggctggcg tatggttgcg gtgactctgt aattggcatc
    17041 aacccttcgg atgattcata ttcttcggtc tcgcgacttt tagaaatgac ctatgacctg
    17101 atcaaggagt gggaagttcc cacccagaat tgtgtgctag cgcacgtcac cacgcagatg
    17161 aaatgcctgc aatctggatc gccggttggg ctggtgttcc aatccatctc cgggtcacaa
    17221 aaaggctgcg agtcatttgg cattaacgtg ggcatgctcg atgaggctta tgaactggcg
    17281 aaaaagcact gcttcccctc aggacccaac tttatgtact ttgagaccgg gcagggatcc
    17341 gcactatccg ctgatgcgca caacggctgg gaccagttga cgctggaagc gcgcaattac
    17401 gggttagcca ggcgctatca cccttttcag gtgaacactg tggttggttt tatcggtccc
    17461 gagtatctgt atgacgccaa ccaaatccag cgggccgggc tggaggatct ctttatgggt
    17521 aaactctcgg gtattcccat gggtgtggat gcctgttaca ccaaccacgc gcgcgccgac
    17581 caaaacgcca acgaaaacct tgcggtacta cttaccgcgg cgggctgtaa ttacttcatg
    17641 gctgtgccga tgggagatga ctcgatgctc tcttatcaat ccacctccta ccatgacaca
    17701 gcctcccttc gtcagttatt ccggctgcgc ccggccgctg aatttgagca ttggctggaa
    17761 tcgattggtt tgatgaagga tgggcggcta actgaactcg ccggcgaccc cacctttttc
    17821 ttaaagcgat gagcgaatga tgatggaaga agagaagatc aatcagattg ttgaacttgt
    17881 actgcgccag ttgcaagaag aagggctggt gccaggcggg ttaaaaaatc aacccggtca
    17941 acccactttt ccacaagaaa ccattacgac cggtgcagcg acagtatttc acccaggcgc
    18001 tgctttatca acccacgcgg aaatagaaat tgacctgccc gaccccgctg gtgataacct
    18061 gcgctggctg ccgcgagtga aaaatgccaa agaccccgga gcgttaatcg aactgatggc
    18121 atcaacctcc gcccgcatcg gcgtaggaag ggcagggccg cgcagcaaca cagcttccct
    18181 gctgctgttc caggcagacc atgccatcac ccaggacgcg ctgtatcgtg atgttgacca
    18241 acgcttgctg gacgagttta acctgatgac cgtgcagacc agaatcacgg agggcagaga
    18301 agaatatctg ctgcggccag atcttgggcg gcaattgagt gacgcggctc gccagatgat
    18361 cacggaaaaa tgccagaaga accctgatct ccagcttgtg gttggcgatg gcttgagcgc
    18421 cctggcaatt gagagcaacc tgcggcagat tttcccagtc attgttcaag gagcccagtc
    18481 agccggttta cgcatgggca caccgtttta tgtgaaatac tgccgtgtgg gtgtgatgaa
    18541 cgatatcggt gatattcttc aaccgaaggt ccttatcctt cttattggtg aacgcccggg
    18601 actgggcagg gcggaaagca tgagcgccta catggcctat cagcccgggg cggggcagaa
    18661 cgatgcaaac cgcgatgtgg tctgtaatat tttcgaaaaa ggcggcacca acccgctgga
    18721 agccggggcg tttattgtgc agttcgcgca aaaaatgatg caatttcaag cttccggtgt
    18781 caaactgaaa ctggcggcag ggtagggagc gtatggcggt ccttgacaaa atacaggcga
    18841 aagcgttatc cgtgcagctt attccgcgcg tggaagataa ttttgcgcgg cggcttaact
    18901 taacccctct tcagctttct gttgggctga tcacctcgaa taacgatgat gccacttacg
    18961 ttgccgttga tcatgctacc aaaatggcgg acgtggaagt ggtgtacgcg cactccttct
    19021 acgctggatc caaacacgct tcagggttgc tttcaggcga gatcattgcc attctggcag
    19081 ggcccaaccc gtacgaggtg aatgaagggt tgaaagccgc ggtggactac cttgaaacgg
    19141 aagccttctg gtactctgcc aatgaagacg cttctgtggc ttttttcccg cacgtcatct
    19201 cgcagaccgg ttcgtatctt tctcgcacca ataacttagc cgtcggcaca ccgatcgctt
    19261 acctggtcgc cccgccaata gagggactgg tggcgctaga cgcagccctg aaagccgcgg
    19321 ctgtttccat cgttgcgctt acgatgccac cttctgagac caattacatg ggtgccattc
    19381 tcaccggcga gcagccagcc tgcaaggcgg ccgcgcagat gttccaggaa aaggtgctgg
    19441 atgtggtcag aaacccgttc agtggcattt aacaataaaa aaacagacga caaggtgaca
    19501 ggatggaact gaccaaacca atcacgtatt ttttgactac cggagaagtg aataccacgc
    19561 ggacatttga acttgcccgc gcccgcgcgc ttgaactggg gttgaaaaca gtactagtgg
    19621 cttcgacttc gggaaagact ggcgcccttg cggcggagtt ttttaaaggt tttgaagtgg
    19681 tggtcgtttc gcactctgca ggttttcgag aagacaacct gcaggaattg accagtgact
    19741 cccgtcagaa gatcgaaggt cttggcggaa aggttctcac cgctacgcac gcctttggcg
    19801 gtataaaccg ggcggtcagg cgtaaattta acacctacca ggtcgacgag atcattgcgg
    19861 gtacgcttaa aatattgggg caggggttga aagtggcgat tgagatcgca ctcatggctg
    19921 cggatggcgg cgcggtgcgt acggataaac ctgtactgtg tatcgctggc agcaacgccg
    19981 gcgcggatac tgcggttgtg cttatccctg cttattcaca gtcttttttt gatattcgct
    20041 tgctggaagt gatctgtttg ccttcgccag agcatcgcga atttcaaaaa taaaaccatc
    20101 aatggttgca ccctctatga tttaaacctg aacgttcacg caccaggaga gccacgcaag
    20161 gacgcgaggg gatggattgc ggcttcagcg gttttgttta ttgcactcaa gcgcgcgttt
    20221 cctaaccggc atcatcacca ctggggcagg tggttcgcga atctgattta atctcccaac
    20281 cgaaacctca agcgcttctt ttctttaaca ttctttccag gtatttcttg ccctcggcag
    20341 ccactgatgg gatcaggatg agcggaagga tgatctccca ttgggcccaa cccagcggaa
    20401 cggtattgaa gagagtgttc aggaagggca cataaatcac caggaagacg agcatcagcg
    20461 agatgaacac accgacattc atccatttat ttttgaataa tccgattttg agcaccggga
    20521 aatattctga gcgtgaagtg taagcgcgca gcagttctga gatagaaaga gttgtaaaag
    20581 ccattgtctc agcaaattca ggcagttcgg ggtgagcgcg cagaccgata aaataggcca
    20641 gcagaactgc ggaggttatg gcgatcgtct gcacgatgat cccgccgcgc atgtatttat
    20701 tgatgatgga ttcatttggc ggtcggggct gttgttccat gatgtcaggg tcaccggttt
    20761 cgttggcaag cgccagcgcc ggggcgccat cggtcaccag attcagccat aacaactgga
    20821 tggctgtgag cggtgattta ccggtgaaca gagtggagag gaagatgatt aagatttccg
    20881 cgatattgca cgagatcaag taatatacga acttgcggat attgctgtaa atgatgcgtc
    20941 cttgctcaat cgcggaaaca atgctggcgt agttatcatc cgtaagcacc atatcggccg
    21001 tttctttggc cacatctgta ccggtgatcc ccattgccac gccaatatct gcgcgtttga
    21061 tcgccggggc gtcgttcact ccatcgccgg tcatggcgac cacctggttg ttggcacgca
    21121 gcgcttcgac aatcttcatc ttatgctctg gcgagacgcg ggcaaagacg tctgtggttt
    21181 ttacggcttc gcggatctct tcggcgctca gcaggttcaa ttctgcgccc gtgcgcacag
    21241 tatgcccagg taaaagcagg ttgatggatt tggcgatggc agcggcggtg ttggggtaat
    21301 ctcctgtgat cataatggtg cggatacccg ctgtgcgcgc cttttccagc gccgggttga
    21361 cttctgggcg tgccgggtcg atcatcccca tcatgcctac gaacaccagg tctcgttcaa
    21421 ggtcttcgat atccagctca gtgtcattga ctttgggcgc gttagggacg agcttgtaag
    21481 ccaaacccag aacccgcaac gcgtccctgg tcaggcagtc gttcgcctct aatatttggc
    21541 tgcgtacttc ttcatttaaa agtatctctt tatcatcaat ctgcgtgtat ttactgcata
    21601 gattcagcac aacgtctggc gcgcctttaa cgataatcgc gtaccagtcc ttttttgatt
    21661 catcatggat cggcgaggcg tcattgaact gcacccctct ggtggaatga atggtgacca
    21721 tccttttgcg cgttgagtca aaaggaacct cgttttcgcg cggatatttt cccttaagtt
    21781 cgcccaggat aacccccgct tttgccgctg ctacaacgag cgacccttcg gtcgggtctc
    21841 ctatgagatg gtagctttca ccacccgcat cgctgacggt ctcgaggtat gcgtcgttat
    21901 ttagcacgcc tacccacaag gttgtggcca gccctgggta atcctcgact gttatttttt
    21961 cccctgcgta aagaaaatcc cccgtcggcg tgtaaccggt gccggtaatt tcaaaatcca
    22021 tcccatcgac ccagatgcgg gtaactgtca tttcattttg ggtgagcgtg ccggtcttgt
    22081 cagagcaaat caccgttgcg gaaccgaggg tttcaacgga agcaagtttg cggatgagcg
    22141 cgtgccggcg caccatttct cgcataccca tggccaggct gatggtcacc acggcaggca
    22201 gaccttcagg caccgcggca atcgccaggc tgacagcgat cataaacccg tccaccagtg
    22261 tcttgatcag gctggatgaa ccctccggac caagcaggtg gcgcacgaca ataaccacga
    22321 aaacgagcgc gcaaatgccc agcgaggccc accccagcac tttgccaagc gaatccaggc
    22381 gtttctgcaa gggggttact tcctcttcaa cgctttgaag catgtcggcg atcatcccca
    22441 gctgtgtttt catgcctgtg ccgatgacga tgcccatacc ccgcccgtat gtgaccgtgg
    22501 tgcccatgaa ggcggtgttc ttgcggtccc ccagggggat gttattttcc aggcggaaag
    22561 acgcgttctt ttgcaccggt actgattcac cggtaagtgc ggcctcgtca atgcgcatat
    22621 tgatggattc caccaggcgc aaatccgcgg gaatgaaatt acctgactcc aagaaaacca
    22681 catcgcccgg aaccagttca ggggcgggaa tattgacgcg atgaccgtca cgcaacacag
    22741 aggcctctgg cgcggccatc ttcttcaacg cagccagcgc ctcttcggct ctgctttcct
    22801 gcacgacact taaaatagtg ttgaggataa cgatggccat gataactgcg gattcaatat
    22861 agtcgcccag gagcgcggaa atgatggctg cgaccagcag caggattaca acaaaattat
    22921 tcaacgcgtt gagcacgcgc tgcaggaagg aagttctttt ttttacagtg agttcgtttt
    22981 taccaaaatg agcgagtctc cgcgcggctt cctctgaggt caaaccacag gttaacttcg
    23041 tttgtaattt ttgttcgact tcggaggaat cgagcgcgtg aaaaccaatg gttaaatcga
    23101 gaattgcagg agactccata tccaatactc caaattttca atggaacggt ggtgaaatcc
    23161 cataaatttc gatgaaaggg ggtcaacttt ctggtagaca ccctgcagca ttgggggatc
    23221 aggtttaccc atcagcctaa ctccgggtga ttggatttaa cgaaccagga tcacaatggc
    23281 gccgatggaa aacagccagg tctttatgtg aggtagtata tatccgcgaa attgtcgtgt
    23341 caagttgaac atatggaggg gtgtgagggg gtcagcacat cgtatacagg tcttggagcg
    23401 tatgagaaca ggtttccaga atgggttgga taaagcgtaa ccgcttggag gtcaaatacc
    23461 atgagggcca tccctctcgg agtaactacc ctcgcagcat ggagggactg tgtatctggc
    23521 attgtcgcgt ctttcgataa agcggcgtat cacagctgcc ggcaaccccg ctgaacggtc
    23581 gatttttagg ctttgtggac acgcagaagg tgctctgggg gaaaggcgcc gctaactgag
    23641 cgtattttta tgagggttga ctgaacggag gaacggacgt accagttacc gggttgtgga
    23701 gaaccttcgg ctctctatcc gcgagcatcc ttgcggtaat gtcagcacga tgttggaaca
    23761 ggttttattt cgcgattttt cgcaatttct tgatgccttt caggaaatgt ccgttcagca
    23821 catctaccaa tccctccacc tgcaagatag acatgctgtc actgcccatc atggtgagaa
    23881 agcgcagcgg catatcattg acgatcttct ccgccatcat cttcaaataa gggtctttcg
    23941 cgcctatctt ggtcacctgc tggttgatca gatgccttat caacctgcca aaccatttat
    24001 cctggatatc agtgatggtg gagttgaccg tgtgaggagc acctttcacc cgttcgcgag
    24061 cgggtatgct ccttccgagg atggtggaaa aatcggcgtc acttaccgtc cacctcgctg
    24121 aggggtggta atatcctggc gcttgagcgc ggaggtctgg cagggctgcc ttaacccgcg
    24181 ttgtgcaggt gaggacagcc tttaactgaa tgtcgcggct ggatgtgccg atgcggacct
    24241 catactctcc ctcttcaacg tgccagtctg ccaggttgac attgtaataa gcgaaatctc
    24301 gttgaaagag ggtgaactca acctgctggc tttccccagg agataggcgc gccttgataa
    24361 aacctttaag ttcctgctcg gcacggatga tgacggatcc ttttttcgcg atgtacagct
    24421 gaaccacctc gctgcctgcg cgggaaccag tgttgctcac ccggcaggta acctttaatg
    24481 tatctgaatc gcccatctta tttgatgaaa gctgcaaatc acttaacgag aaggtggtgt
    24541 aggatagacc aaaaccaaag ggaaaacgca caggcacatg ggcgctgtcg taataacggt
    24601 aaccgacaaa aataccctca cgatattcca cggtaagcgg gtaaccgggg aaataattga
    24661 aagaagggtt atcctccagt tggaacggcc acgtttcggc gagttttccc gaaggattga
    24721 cacgacccaa taacaggtca gccaccgcgc cgttcaccgt ttcacctccc aggtgcatca
    24781 gcaggatgga tttaaccctg tcagcccagg gcatctcaac cacgccaccg cagtagagaa
    24841 caacgaccac gttgtggttc acctcgctca cagcttcgat caggcgttcg tggctctgcg
    24901 gcagggcaag tgtttcacgg tcgaatgtct cggattcaaa gcggtcaggc agtccgacga
    24961 acaggagaac cacatcctta ccggcagcca cctggcaggc ttcagcgaga agcccctcat
    25021 cgggcgcatc agcatcttca aagtaacccg cagcgtactc aaatcgcagt ccgagcgatt
    25081 tcaattcttc aacagcgtca tttacccgta tgggggtgat gcgcgaacta ccgcttccct
    25141 ggtagcgggg atttttggcg aaagcgccta tcagcgcgac ggaagaggaa gggtttaatg
    25201 gcagcagatt gccttcattc tttagcagca cagctgattc agctgcggcg cgcctggcaa
    25261 gcgcccggtg ggcgctcacg ttatattgag cacgtttctg ccctcgcgcg cgcagcagaa
    25321 gcgcggtgac attttcggct gtctgattca gtaatgcaac aggcagatcg ccggctatga
    25381 ccgcctgggc gaccctttcg tcgtgcgtac gatcgaggtg tggcatctcc aggtcgagcc
    25441 ctgccgccac cccttttacc ctgtcgacca ccgctcccca gtctgaaatg accagccctt
    25501 cataccccca ttcctggcgc agaatctggg tgagcaagta ttcattttgg ctgacgaatt
    25561 cgccaaacaa gcggttgtaa ctgcacatca cggtccaggg tctggcctgt ttgattgcgt
    25621 actcgaaccc agacaggtag atttctcgca aggtgcgttg atccatcacg gcgtcgacag
    25681 ttaaacgtct tgtttcctgg ttgttggccg cgaaatgctt aagagaggtt cccactcctt
    25741 tgctttgcac gccttcaata aaggcagcgg ccaggtggcc tgaaagatag gggtcctcgc
    25801 tgtaatattc aaaattacgg ccgcacaggg gcgaacgctt gatattcatt cccgggccca
    25861 gcagtacaga aacaccttcc tgcaggcatt cttcagcgag tgactggcca acttctcgca
    25921 gcaacgccgg gtcgaacgaa caagccatcg atacggcggt tgggaagcac actgccggta
    25981 cattctcgct gatccccagg tcttcactgc tcgaaagctg cttacgcacc ccgtgcggac
    26041 catctgtaac catgatggaa ggcaacccca accggtcaat tggtttcagg taccagtaat
    26101 ctttgcctga gcaaagagag gatttctcct ccagggtcat ttgtgccaca atctcgcggg
    26161 ctttggctcg taagttcatg gttttcactc catgccgacg taattaaatt cgctcatttg
    26221 caatttctcc gcttctgttc atatacaacc aaacgcaaag gctcgaacat cctctcttat
    26281 ccttgagcaa acagccctgc ttgaaaagag aaaagggtgc acttttactc ggcggcgggc
    26341 tgcttcttac cctggtaggc ggacctttta ctgcttactt gttcagcaac ctggctcgat
    26401 caaagctttg ttggaaaaaa ttaaagagaa tttccacgcc gtaaaccagc catcaggttg
    26461 cttgaaaagt accatcggcg atacgagagc cgcaagaaac ccaaacgcca ttctcatttt
    26521 acactcgttt ttttctgaca gccggcggca ccctcctgcc gctacctgtg cccaggttca
    26581 ggtgcgtgtt cgacgagctg gttcaatttc tcaccggggt ttgtcacctt cggggatttt
    26641 gaaaatcgcc ccgttggtat aattaattca cctgctgaga ggctcattcc ccccaagcgc
    26701 ggttactgtc agcccatgac caccaataag tacatcaaag cggtttccta taacatcgca
    26761 tattttctgg taagctcgtt ggcttacctg gtgctgacgc ccatcgccat ccgtgtgatg
    26821 ggcggtgagt tttacggtct gtggatgatc ctttttgcgg tcgggcagct ttcgcttatc
    26881 ggcacgttag gaatcgagac tattgtcaac aagttcgccg cagaggtgaa agaaacccat
    26941 ggagcggatg ccaatcttct ctcttcagcc atattggtta cgctggcgat ggctttcatt
    27001 gtggcagcga tcctgtttac tttccgtggc ttcctggcgc agcgcattgg accctcaccc
    27061 gcacacctga cccagtttaa aaacgccttg gcggtatact ctttaatgat catcccgcag
    27121 ttcacgattc gagtttttca tggatttttc ctggcgcaaa ttatgaaccg ttttgtgcgc
    27181 ctaatggacc tgctgaactc gctcttgccg ttattaggag gggtgatcct ctcgcttgtg
    27241 gcgcaaaatt tgaccctgct ggccttgtgg aacttatttg tcagcctgat agtgttgggt
    27301 gtctttctct ttcgcacata ccgtttgatc ggtttacgct ggctgcctac tcgcgtaacc
    27361 accaggcgcc tgttgggatt ttctcctttt atgttcattg agtctgccgc gatcaatctc
    27421 ttccagcagt ttgatcgttt attggtggcg ttaatgctcg gtccagcggc atccggaatt
    27481 tacagtgtcg ccaccggtgt ggggctgcgc atgtcgatta tcgctggcag tgtgaccgaa
    27541 gtgctcttac cttacgccag ccgcaagcat tcacttggtg agaagcataa gttagaaaat
    27601 attgtgctta aggttacgcg ctatatcagc ctgatggctg cgctggtggg tggtttgctg
    27661 atcatttgga tgaaagaatt gctcaccgtg tggatctccc cgcagtacgc tgaagccaat
    27721 gcattgttct tctgtgtgat catactggcg tacagcatac tcagtctcag ccgcccggcg
    27781 caccagaccc tggtggggtt gggcaaggtc aggttcacct ccctggttta cctgggttct
    27841 gcggcactca tgctggtaac tctctgcctg ctttcaatga ccagggggtt gcttggcgct
    27901 gggtacgcca acctggtgat gacacttttg ttatgcatga gcctttattt attcaagtgg
    27961 tttgaggatg atcaggtttg gcggtatttt gccactgacc ttatactggg tatgatgccg
    28021 ttggtggtgg cgcttattct tgtcggtatg agatgcgctt tgccgcttaa atgggttttc
    28081 accattgggc tgttggggtt ggttttcttt gtccttgcca gggacgatga gctgaaagcc
    28141 cgcctgaaac gactggtcac aggttcagct ttggccaagt cgtcggagcg tggttctcag
    28201 gtgaaagaca aatgagcacc ccaggcggag aacgcgaaat ggcgaagatc tgtgagcatt
    28261 tcaaggcgca ggagttggaa aagtgaagct ggttttttgg ggtaaatata ttgacaccta
    28321 cctggctgat acactgcagc gctacgaaga atctaccagg atgccggtgc aatatatcgt
    28381 ggtggagggc gagttcaccc gccgaaaggt ggctgctacc caggctgtca aaaatcgtga
    28441 tcagttgacg gttttagaaa aaagaaaacg ccaacaacag gccgaccgtg tccttgccgc
    28501 aaaccaagac gcggtgcacc tttttctcag cttttgggga gacaagcggc tttttggcgt
    28561 gctgctgaaa gcgctgcatc gcggacgaaa cgtggcagtg gtcttcgagc cttacgcgag
    28621 cgttcccctg ggttattgga aggatgaagc gcgcatcctt tctcatttta aaatactgag
    28681 ccgacacatc gcctaccggt tcttatggcc gttgataaag cgcgctgcta ggggtccgct
    28741 gccctgtgta ttggcggttt ctccactggc gcaagagcaa ttgcgcaagg tgggtttccc
    28801 ggaggaggtg attttcccat tcgggtattt taccgagcgt aaaaatggca tacccgttca
    28861 acggaaacag gcggaagttt tgcgcgcgct gtttagcggg actttgatca ggcgcaaggg
    28921 gctggatatc gccatcgccg cggtgcggcg ggttaattcc cccacggtca aggttcagct
    28981 ggatatttat ggcgcgggag aagtaaagaa atttctcgcc cacagcgaac cgggaatctc
    29041 ctataagggc gtctaccttc aggaacaggc ccagcgcgtc atcagcgctt acgacttgat
    29101 gctcgttcca agccggcacg agggctgggg gcttgtggta aacgaagcgt tgatgcagtc
    29161 agtgccggtg tgcgccagcg accgcgtggg cgcgaagtgc ctgctggaaa cttcaggcgc
    29221 tggcttaacc ttcagaagcg gtgacgcggc tcacctggct gcacttctcg ctcacctggc
    29281 agcggaccgc cacgcgctgc aggtgatggc gcagaattgc aacaggattg ccaacctgat
    29341 cactccttca gccggagcgg aatacctcag ggaggtcatc caattctatt ttgcagcttc
    29401 tggccgcaag ccgaaggctt tttggctgaa cgcttcacca gaaaaccctt caccggacat
    29461 gttctctcac cgtggaaaaa gcgggaggta aaggaccagg cggcttgaca aggtctcctc
    29521 atttaacaat ttcatctctc agcaaccagg ggtttcgcgg cgaagtataa ttaacttgct
    29581 tttatcatct caatcttgtt gaaaaggaaa aaatgacgcg gattgcgcac atcctggggg
    29641 gatggtctgt caatatcggc aacgcctttt atcaactggc agggtatcag gtgctgcgtg
    29701 aatgcgcccc cggagccgag attacactta tccctgagca gcctggttac ccgtcttact
    29761 ggaactggaa gggcggaaac cctggtaacg ccttggagta tgttgggcga gttgcgccag
    29821 attacctggt gttgatggga ccagtttttc gccgcgagat tttcaacatc tgggcagatg
    29881 cgctggaaag actcacgcgg gctggcaccc ggctggtgtt actgggggtt ggcgcgatga
    29941 cctacgacgc tccgcatata cacctgtacc gcgagttcct gtcgaggttt cgccctgtat
    30001 tgatggtgac ccgtgaccgc gaaacttacc atttgcttgg agacctggca gaacacgctt
    30061 atgacggcat cgattttgct ttttttctcg ccgattatta tccgacctgc aggatcgagg
    30121 gtttttccaa taccctcgcc ctcaatttcg ataagatccc cgaaccgaaa attgcgacag
    30181 aaggcagcac ttcttccccc ggtgcttctt ttcatttcgc cggtatgcac tggcaggtga
    30241 agtttccacg cttcagaacc tggctggcgc gcagttctcg ttacggaatg ttcttggaag
    30301 gctgcctgtt cggcgggcgc aatgagttaa aggtgggtgg ttttgatgtg atccgcttgg
    30361 accatcgccc tcaccccttg ctgcgcgcta aaaccttccg ttcgccgcgc acactggtga
    30421 acgacacgcc ctacccgtac ctggacgttt acggaaatgc cgcgctcacc cttacagacc
    30481 gtgtgcacgc tgctgttgcc gcgctgcaat atggtaaacc ggcgatgttg ttttcttctt
    30541 cgccgcgggt gcgcctgctg gaccggctgg acttggcagg catcggtgag cagcctgtat
    30601 ggctggatat ggaaaggctc agagaagaaa agacaaacat cagggatttt cttaaggcga
    30661 atctcaaaac ctgacctgct atgaaggtaa ttgagaacag cccaagcggg tgatgatgcg
    30721 gcagaaccac ggatttgaat aaggaaggcg agcaatatgc ctgcaacgcg gcaggagtta
    30781 tggataaggc gataagcagg ttttcataag aaatataacc ctgctctgag gatgaatacg
    30841 aacgaaatac ggacaggtga gtaccggaag gtgaattcca gaataagcaa gttgatcgct
    30901 ttgctgcgca atccttttta ccggaggaca ctgttcaggt atcgtgtggc ggcggcggtg
    30961 gagcacgacg cgctgctgaa aaggctgggc aaagatttgc gcacggtggt ggatattggc
    31021 gccaatcgcg gtcagtttgc gcttgtcgcc cgttttcacg cgccagaagc cagaattgtc
    31081 gcctttgaac cgctggaaga gccggcggag gtctttcaag agatgttccg gctggatgct
    31141 ctgatggagc ttcatcgttg cgcggttgga gcccacgcca gtgaagcggt gatgcatctt
    31201 tcgaaagccg atgattcttc ttcactgctg ccgatcacag ccctgcagac cagggttttc
    31261 ccgggaacgc aggaacgcgg tacccggcgg gtgacggtta gacccttgac ggacgtgctc
    31321 ttgccacggc agattacacc tccatcgcta ttaaaaattg atgtgcaggg ctatgaactt
    31381 caggtcttag agggcggcgt tgccctgctt gattgctttg attacctcct ggtcgaatgt
    31441 tcttatgcag tgttttacaa agaccaggca ctggcaggcg aggtgctcag ctacctggaa
    31501 aaagttggtt ttgttcttat acagcgctgc aaccttattt atgatcaaca tggagtgcca
    31561 cttcaggcgg attttttctt tcaacgcaag taagatacaa tcattcaagg aacagtgtca
    31621 ggacgatatt gaccaacagt ttggggagcg aaacattgcg cattttgctg catgattatg
    31681 gcggttacag ttttacaaac cagttaggaa ggactttggc gggtagaggg caccgggtac
    31741 tgtattcgta tagccttaca acccaactga tgcaacgttt tgcaccgcga cccgcgcatg
    31801 aaaacctggt aatggcggga gtacagctac cccgaacgtt caatcgctac agttacctgc
    31861 ggcgttggcg ggatgaacgg ctgcacgggc gcctggtcgg agagcaggtg aggaatttcc
    31921 aaccggaggt tgtgctcacg gccaacaccc cgcttgacgc gcaggtgctg attcagcgcg
    31981 ccagcaggga agtgaaagcg gcgtttcttt tttggatgca ggatttgatc gggctggccg
    32041 ctcaaaaaaa cctctcctcc cgctttccat tgatcggccc tctgctcgga gcgcattacc
    32101 agcagcttga aaaaaagttg gtcattgcca gtgacgcggt gattctcatt tctgacagct
    32161 ttttcagtta cgtgcgcggt tggggcatca cggctgagcg tatgcatcta ctccccaatt
    32221 gggcgccgct ggaagagatc cccctgctgc ccaaagagaa tccctgggca gaagcgcaag
    32281 ggctcgccga caaattcgtc tttctctaca gcggtgtact ggggctgaaa catgatgcca
    32341 gcctgtttct cgccctggcg gaatctttcc gctccgatcc tgaggtgcgg gtggtcatcg
    32401 tggctgaagg accttttgcc gaaaaattac aggcgcaggc ctgtgtcagt gcccttgaga
    32461 acctggtcgt gcttccctat caaccacctg cggaatatcc ccaggtgctg gcaagcgccg
    32521 atgtgctcat gaccattctc aatactgatg ccggcgccta ctctgtgcct tctaaagtca
    32581 actcgtacct ttgcgccggt cgaccgcaac tcttgtcgct gccctgcgat aatgacgcag
    32641 cccaactggt ttcttcacgg aaaatcggat tggtctctga gccgggagat gttgagagct
    32701 ggttgatgaa cgcgcgcgcg ctgtatcaga accggtacaa tcacaaccag atggcgatgt
    32761 acgcgcgcgc ctacgctgaa gagaattttg atattgagcg cattaccgat aaatgcgaaa
    32821 ccatgatgaa gttagccttg agaggtggtt gatggcaggt tcaaatggag ggcaggcatt
    32881 ggcggcatcc gcgcagaccc gcttttttga ccgccataaa cgcgcgttca tgttcgctat
    32941 catgctgctg gttgacgcgg tagcgttgct gctcgctgcg tggctggcag tgctgatccg
    33001 ttctctcttc tcgccagcgc tcactgaccc tgcgctttat tacgcgcttt tcccttacct
    33061 gctcgtcttc ttgctggcga attttttcac ccgcctttac cctggcaacg gtatcactcc
    33121 tgatgaggaa ttgcgcggct ccacttacgc gatcagcgct ttcatgatcc tgctggcaat
    33181 atttcttttt ctcacgcagc gcgggttgga attctcccgc tttgtgtttc ttcttttctg
    33241 gttgctggca gtcatttttg tcccgttgct gagattgctg gcgaaatact ttggccaccg
    33301 cctgggttgg tggggagagc ccatcgccat cctcggcttt ggcgagaagg gacgcacagc
    33361 ctatcacttt ttcaaacgca attccctgtt cgggctgaac cctctcttga tcgtccattc
    33421 atcaggaaat gaactgcggg aggaatcgga tgtgagaggg gttgcaacac tggatgagga
    33481 tatgttggct gccaaccctg aactggtgcg cagattgggc atccgtatgg cggtcattgt
    33541 tcctgatgaa gtttcagcga accttaactc cctgttgttg gacgaacaac cgttcgggct
    33601 gaaacgcctg gtactgatct ctccctttac ctggctgggg ggcccggtag tgacggctca
    33661 ccacctggac ggcctgcttg gactggaggt ggaagccaac ttacttaaac cgcttcagcg
    33721 ggccatcaaa cgctgtatgg acctggcgct gggcgtgatt ggttttattt gttctttgcc
    33781 gctttttgcg gtaatcgcgc tgctcatcaa actggattct cccggcccgg tgctgtaccg
    33841 ccatgagcgc gttggcaaag gcggcaagcc catacgcatc tggaaattcc gcacaatggt
    33901 gcagggcgct gacgccctgt tggccgagct tgtcgcccaa aacgctgacc tgcgcgcgga
    33961 atgggaagcc aatcacaaat taaagcaaga cccgcgcgtt acgcgcattg ggcgctggtt
    34021 gcgccgaacg agtctggatg aactacctca ggtgatcaat atcctcaaag gcgagatgag
    34081 cttgattggt ccgcggccga tcgtgcggga agaggtgaag ttctaccagg agggtttcgc
    34141 gctctaccag aaggtgcgcc ccggtttatc ggggttgtgg caggtctcag gccgctcaga
    34201 cacgagttat gcttaccgcg tttcattgga cgaatattat attcgccact ggaccatctg
    34261 gctggacatt ttcatcttgt tgaagacgct gtgggtggtg gcaagcagaa cgggcggata
    34321 ttgaaatacg cgctgaaaca gcaaaacgcg gccacaggcc gcgttttgct gacttgactt
    34381 caggcggggt ttctacaact gtctcatttt cctgtcttgc agcttgtgag gatgccctga
    34441 agcgcgtagg tcaaacggct ttgtttacct tcgagccgat ccaggtagag ccagtaggcg
    34501 ccaagcccca gcagtgcgcc gagagtatcc agggctaaat cgaccaactg gaacgctcgc
    34561 cccggaatga agtactggtg caactcatct gtgaaggcgt aaaacagaac gagggagatg
    34621 gctttgataa attgcccgcg agtgggtctc tcgcgctgcc agagcgcccg cgcgaataag
    34681 aaagacagta tcgcgtattc aagtatgtgt gagatgccgc caaggtaaaa aatgagcgaa
    34741 cgcccgaaaa gtttgctcca ccacagccag ctgtacagcc ggttggggat gtggctgtaa
    34801 gggtttgaat cggcagagag aataaaaatc accgccgccc agaagagcac aggcagccag
    34861 cggcagagga acggcttgag cgtcttacgc gcgcggttgt ctattgaacc tccattgcct
    34921 tctgcaggct gttccagacc agtacatccg gacgcaggta agacgaataa tactggtggt
    34981 tccgctgcat catcgctaat ctcaggtcaa aatcgtccac cagcctgcgt acctgggcga
    35041 cgcactcttc gggcgtagcg aaggaaaggt agttcaatcc actggaaaaa tcaccgggca
    35101 cctggtaacg caggcgttcc gacacaatcg ctttcgaagc ggccacatat tcgcccagct
    35161 tcccgccatt tgaaccaaac aaccccatgg ttgcaatgcc gatatcggaa ttgtgcatga
    35221 gggcgagata tgccttcttg tggaagacgc gcctgtcagg gatcacgtag ttaccgtgca
    35281 ctgtccgcgc gtaatcgctt gggtagatac cgccgaaaaa ttgctgagga aattcccgcc
    35341 gaagcatggc aatgcactca cagcgcattt tattgatgtc caaccgctct tcgaggatct
    35401 ggcgatcggt gatattttnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    35461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngc
    35521 ttatcccccc agcgcttcca aaaggctatc aagcctaccg cgaatcccaa accataactg
    35581 aaatgcatgg tgatgaaggc gattggcagg agaaagaaat ttttccatcc tttttttatg
    35641 gatattcgaa aggaagccag gatggcggcg aagaggtacg ccccgagtat gctcgctaac
    35701 aagacccacc cctcgtttcg gaacaaggca agcgcgagcg aaccgaagat gctcaggata
    35761 aagatcaacg ggataaactg ccgggcgctc atctgttgag ggtgtttttg caatactctc
    35821 accttgtaga acccgtattg atagtattgt ttccataaac ccctcattga tccccggctg
    35881 tagtatacag acctcacatc atccgccagt aacagctttc cacccaactc gcgtatgcga
    35941 tagttgtact catcatcctg gttgcgcacc agttcttcat cgtacaaccc ggctttttgg
    36001 atgacctctc tcttataagc ggggaagggg atcgtgtcga catatttcgt ttgtccctgg
    36061 gtagtacgga aggcggaatt gcccacccca aagcgggcgc tcatggcgag cgcgatggtt
    36121 tcagccagcg gagttagccc gatggttttc attgggccgc ccacgccgtc cactgtatcg
    36181 ttcgcgaggt gtctcaggca attctctacg taatcaggcg cgatgacgca atgtccatcc
    36241 acgcggatga tgatgtctcc cgcggcctgc cttaaggcgg tgttcatgcc ggtcgggacg
    36301 atcctgccgg ggttatccag tagtttgatg tttgcgtgct tgtgctggta ttcacggacg
    36361 atctcacgcg tgccatctgt ggacatgccg tcggcgataa ggatttccat ctgaccggcc
    36421 gggtaatttt gcgccaaaac agcgtctaac gccgcgcgaa tggtaggccc ttcatcgcgg
    36481 atgggaagaa tgagggtgac ctttttgttt tccgcagcgc tcatctttca ccggtaagtt
    36541 tgcgcattga tcatggaaac gaacggttta aatttcccat tggcgttctt ttcaatctcc
    36601 gtcacttctg tgagggtgat attcaccgcg ccaaggtact gctgcaaacg cgcggtgatg
    36661 gcgcccagca tcgcgcttcg gctgtaggtc tcatctggaa ctactttcac ttcaaatgaa
    36721 tccagggtgt gttggatcaa ctgcccttca atcaccggca gaccgttata cagggaatcg
    36781 aggatgtaaa gttcacgacc gtctttggtc agcaccaggt cgttgcttct gccagcgata
    36841 ctcgccagcg agggcagttt gcgttcgcag gcgcattggt gctgccagtt gggtaagtag
    36901 ccaaggtcgt gattggcgta acggaccagc ggcatgtcgt cattcattaa cccggtcagc
    36961 acgtattcac ccgctgtgcc aggttctgtg gtaaattctc gtttttgagg atcaaacact
    37021 tcgataatgc cgctttcggg ccagtaatgt tgcgtcaacg ccaaacattc gctggctgcc
    37081 gcgcaggcct ccgccatgcc gtaagtgtca atgaccgggc agttgaacgc cctggtcatt
    37141 aattcgcgat actttggcag ggtgttttct gagttgcaaa cgatcacctt gatctgtgga
    37201 gggcgaagcc tttgctcaag gatgaacttg gacaatatcg cgcaggagga cgggtacacc
    37261 atcaggtgaa ccgggcggta tttccacagg gcgcgcacgt agtctttcgc ggtttcaggg
    37321 ttgatgtgga aaaccgagaa atagacctgg tttaacccgc cattccacac ccagtaaggc
    37381 gggcggcgct ggtgaagcgg aatgaccttt tgccctccca gcatccccca gcggtcacgg
    37441 tgatgaacgc caaaccagcg ccttgtgcgc gcttcgtgca gcgcgtagat gttcctgatc
    37501 acctcgcggc tttcgaagat cagcgttggc ttgccagtgg tgccgccggt atggtcgata
    37561 tacaggtgtt tcgattttga attctctgaa aggaaggcgc gcgggttgtc tcgcacttct
    37621 ttcttgtcca gtatgggcca gttttgcagc aactcccaac tcgccttatc gccatttctt
    37681 cttctgcgct gccactgctc acgataatag ggcacagtgg tggcagccac ctgtaacagg
    37741 cgcgccagtt gaacttgctg ccatttctcc cactggtttc gatcccagga ttctctttgc
    37801 agcgcttctt ccactaacgt ttctgtttga tgatcaaacc tgagatggcg taaatagtat
    37861 ccccaaacac tcgccgcgag gttcttcagg ggagacggca acacctggta aacgcgttgt
    37921 ttgatcacga ttttaacaat ttattgaaca gcgcttccca ccgcgtgagg atggcggacc
    37981 agtcgtaagt ttcggctttc tttctcgcgt ttgcactcaa gcgagacgcc agttctggat
    38041 cggtgaggat acgtttcact gccgctgcca ttgcttgggg gtcgttaggc ggaacgagaa
    38101 gggcatcgac cccatcttcc cagatatagg gtaattctcc cacattggtg gtgacgatgc
    38161 aaagcccaca ggccgcggct tctacaaccg cgatcccaaa gctttcgcaa ttggtggtgt
    38221 tgaggtagat atcaggtttt gtaaaccatt cctgcagttg cgaacggctg atgaacccgg
    38281 tgatggtgat gttgga
//
LOCUS       DAOTLW010000015        36884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1351_length_36884_cov_9.903962, whole genome shotgun sequence.
ACCESSION   DAOTLW010000015 DAOTLW010000000
VERSION     DAOTLW010000015.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 36884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 36884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36884
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1351_length_36884_cov_9.903962"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            1..251
                     /locus_tag="P5516_03880"
                     /pseudo
     CDS             1..251
                     /locus_tag="P5516_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086558035.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
     gene            complement(673..1968)
                     /locus_tag="P5516_03885"
     CDS             complement(673..1968)
                     /locus_tag="P5516_03885"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRT91610.1"
                     /translation="MITFVIASVNITPVSAAPPSMFVSPASQTVLNCEESVIAIRVTD
                     IYAPGLYGYDVKVSFPVGKLEVVGVSNATFLDNTGYYIRNQVDVISGNVQVWVAYTQL
                     APSTHKTGDGDLIYIRVRALTTGETVPITITYQKLATGDGQSIDIPSNRIFNGTVILS
                     DDCPPVALTINPAETLVCQIGGDTIVNVNVRYAVNLWAYDLQLSFDPKALNILEVKNG
                     SFLQDGIYGHRIWDNDAGTIKFAMSQINPQTPKSGAGTLLTIRMRPKPDQLNKNVRLT
                     ITSGTELMDSSIHNLPYTTADGMLYTNACDPTSADLTNFTARDGVRSVTLRWETTNEL
                     DNLGFNIYRANFVNGKRVKVNDLMIPSKQPPGSMVGSLYIYVDTGFTVKPGNGGGGGT
                     VLHQAKNGRTYYYWLETVSVRGDTELNGPVEVRVFNKQP"
     gene            complement(2006..2890)
                     /locus_tag="P5516_03890"
     CDS             complement(2006..2890)
                     /locus_tag="P5516_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRT91611.1"
                     /translation="MTLYKKNNSVSNKFITKTIFQACFVMLLLMGFTNARAQKLETLF
                     GVMEPAVVAPGATVQVPVAVKDVEGLYAVDFSLEYDPTIVQLVDADAIQPGIQAGLGT
                     FLDPGLLVFNTADNQKGEYRFVMSQYNPSLPKSGEGNIVVLTFKGISTGESNIHFTGV
                     ELLSGEITAIQAKVLDSNIQVISGAATQAATIAVAQATGLIILPKDTPTPTVTATTIP
                     TRTPEPQAAPGGENQTGNGGSTATGGSEPGRPAAAGKGSGLANGWPLIAVFIVLLAGG
                     VTFMLKKKTRKKVEENHE"
     gene            complement(2887..3933)
                     /locus_tag="P5516_03895"
     CDS             complement(2887..3933)
                     /locus_tag="P5516_03895"
                     /inference="COORDINATES: protein motif:HMM:NF013156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cohesin domain-containing protein"
                     /protein_id="HRT91612.1"
                     /translation="MSKRIIRILFVMTITLSFLLGVADTGEFTAQAQGDTEVSIEPVE
                     NNITVGALVELKVNVANAVDLNAYDLKVVYDKEVLTLESWSHGVFLSNVAVVYRDEKP
                     GSLRLAVTQLASPGATGNGTLLNLRFRGKSLGHTTIEIQDAQLVNIANQRIYPTARNG
                     VVEVSTQGSIYTATATHTTTNTASATSTHTPTASATMNPFITWTPTRTRTPTAIYGRT
                     ITPIYAIVITRTGTPGTAVVVPPAGQVTTATPFSIVTGPEDAITGGGAAGIPENVLSN
                     GTVTDGALQQTTEMKRSTEYAHLTAEWKNATRLDEFLHKTRNANGALWGMGFLLLAVI
                     TGLTVLFFNLWRKM"
     gene            complement(4118..11362)
                     /locus_tag="P5516_03900"
     CDS             complement(4118..11362)
                     /locus_tag="P5516_03900"
                     /inference="COORDINATES: protein motif:HMM:NF024627.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HRT91613.1"
                     /translation="MKTISKIFVIVVILGFVFGLVSAREAKASTLDVCASGCTYSTIQ
                     DAVNVAVSGDVISIRAGSYEGFSIVGKSNITISGAGKGQTLIEPTSLINTGVGHKYVT
                     PTLASVFVNQSNNVTIEGLTVKSTSATPGSGGADAIVFWNASTGTITDCSIEGIYTIS
                     GNQTGQGLAVDAGAGETTSLALVNTDISGAQKNAIDVVDGKASTPNAGTISLSITGGS
                     FTGAGPTSVNAQNGIMAWNRGGGSIDLSVSGATISGFDYTPTNNEATGILVYGVGGGT
                     VSVSGNTLTGNEINIYDNTSSLDMDAIFTANTFGPVALIPGGNSILGTIQGAINEATD
                     GDTINIYGTHVLSSMVNVDKAVTLSCDPGALIQVSGTGDRFDFSADGASLVGCQIEKT
                     DKTGEQNIIRIRASDITIQNNKIWGRYVIGDGDVSRAMIINSGVFTDLLIDQNEIYDL
                     RQPAYISGTHSGTISHNYVHNTRGWVLEGGNLTFSNNTWAGNVLDVAILSAMPAAYYP
                     DIVAMSNANNGAVIEDQRHSPALLSVVYVDDDAPAGGNGGQEAPYQTIQEGVNRVTPG
                     GTVHVADGSYYPPAVINVNKAMSILGPASGEAKVYGVGGSTVKVFEITSSDVTISKLT
                     ITLANAPSAHIGMINVPDGPRSNINITDNKIYVDPQPGAMSTWYGQAVYLGRYITGSN
                     VSGNTIYNTRGGIVVHYNSALTISNNVIYNTKGGIMNYTGTQADADARVMTGNSWNGI
                     HNEWDIVWNSGGGPYAMDMNKSVLQVSQANNDGYTISLMTTGSIDNRSHVFVDDDSSF
                     ATAHKSRGNFNEPFKYMQLGIDAVVPGGTVYVAAGNYNEAVTANKALKLIGAGKETTI
                     VDGTGLSNATLFRSAGLTSGVLDISGFTFKDDPDPNAAMTYLVSFGGCQSPAAVSFTD
                     NTVLGTGDTDKDWGLLAQHGTCSFTINDNLFDNIGGNSIMVENSTGAVEIGDNEFLVP
                     NDGGPAIWSMAYGSSLVPNTVTGLHWYHDNYIHPKAAGGSIWSGITIAPAWGSGWNVR
                     QYGSYTNALIEKNKVEGFQSVGIQVEVDGTNSTFNGVIQNNTLTATPGVSSSKGIRLL
                     GPVSDTTLRKNIVTNAHRGIWLSGTWGQPVYPTGITSEKNQLADNDIGYEEQDTASDQ
                     NASPNWWGSASGPAAGYVVSNLKYAPWCTNPECTAFYGPVHNVTQDTYFGSIQAAIDD
                     AATVDGDVITVEAGTYEEQLSIEKSIDLRGPNYGVSAGVAPGVRVPEAVIVFPAGLTD
                     YQEMIFIDAENVKVDGFTLDGQALAEDVDIAGIVSEEKNLTVKNNIIKNFNYIGILSS
                     SYEFVGGSWDRTFYNTGLLIENNLVTNEVPGRYYNYSAIYVQGDNGVVRGNVVDKAYR
                     GIQIQPYGNPETAQGLVENNEVRGYRTGIWFNYSEAPEVNWLIKGNKSVGIPAPATDP
                     VTEWDGLLVQAFYAGNVAFEDNTVQLGATNAPNAYMFKKLAHTGGTIDLEATLANNDW
                     EKVVVVRDSLGNIEETTIPSVTDRETTFFSSIQSAIDDVYTGTGDTVDVPAGTYVENV
                     VVNKSVTIDGAGAGTIVMPAVSNPGVGLPGSLGGSNVFLVKAHNVKITDLVIDGDNPA
                     LVSGVVVGGADLDARNGIITDHTAGVFTGLEVSYVTVKNIYFRGIYASSASSGATFNI
                     HHNNVSNTQGTGQSFAIMVYNGTGTVADNVIDYGKAVGANYSQGTQWLRNTLTHTPGG
                     IHTDNAGFPNILGPEVISGNNVDCDNQASGYGIWTFVNRMPITVENNTVTGCGVAYSA
                     WGSGNTSVATFQNNSATAPVPNNNSVGAYITTSGLGWGYYDVNANFTGNTFTGFASGV
                     VLSAGPASWEPSPYVAKTITSDIKRNNIVGSTQYAVYQGAQGIYAANLKENWWGSLLG
                     PQAPITDNVDVIQWCGTANPTCLPLMPQNGNNLSLSGTVNLETNGLYVPGITYLLLDG
                     TTIQNTDEACFNVFADHISIMAESKLGAKCVPAAGKNGVNVAAGLQDVNIANLEFDGS
                     AGGVSGVLFSGVVDYLTILDNWFHGLTGDAIEFQDQPTGIISIQGNLFQENDGRGIQS
                     YGAIIPAEFNAWGDIDGPVAGDGVSTGVDADPWTHVDLYLYSTSPASIGGQVTYTVYG
                     NLANVMGAGFTLAYPAAKLQLVPASLTNLSGFVEVAPGAGVFDTSTAGEIRFTGKVPA
                     ATPISGKSLPIYSVTFTVLGDDALLDLVEAGAEFTMAPVTADPTWNPAYSTNIYHMGF
                     LDALVTTNSYTVQGTLTMEYRYMNVRSGIPGTLTGGTYGLYMANSVQPIPYNMKFLNV
                     APGTYTFTTNQPRYLNITEDLAKTITVVSSNVTFSTPLTLIAGNANWSDNVIDIVDAS
                     LLGNKYGWDVSTQGDTDADVDFSLKVGLSDLAIVGSNYGKSSAVNYTWMP"
     gene            complement(11717..12526)
                     /locus_tag="P5516_03905"
     CDS             complement(11717..12526)
                     /locus_tag="P5516_03905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91614.1"
                     /translation="MNQKSTLKKIGVVLAAAVVILALIVMLTFVINGPAHMLTSKEIA
                     LEQTRISLHILIKQTEVARTSTAELYLTLGVPFPTETPLIVPTSGSLGKPFGTLNPAN
                     NADGRVAEGGLGQPAQTGLPLSDSVLPQSTQPPFTATATGHPAQNRTATATPAPSANP
                     LAGWNGKWTVYFGADWEELTIGEVNVTVSGTRLNAVGDFSHGRMTISGEISADGQQVT
                     GDYSFNGVSAPFFWQLYGPGQFGGNMAGSRQFCGSKGNLPKPEACGIYFEQ"
     gene            complement(12566..13819)
                     /locus_tag="P5516_03910"
     CDS             complement(12566..13819)
                     /locus_tag="P5516_03910"
                     /inference="COORDINATES: protein motif:HMM:NF026257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HRT91615.1"
                     /translation="MAFSQPTMSLINLLAYPWEGTLAEKTSPLAPEDWRALLEEAEKR
                     ALTLTLYYRLRVAHGEALVPEWVWEHLQTVYLAATARNMVMLHHAAGILRALQERGIE
                     VIVLKGLYLVEQVYPEIGLRTFSDLDLLVRREQLADALTLMQNQGYRLSTWYDPQAQN
                     TDIKHLPPLEKAQHPTLELHWSILEEDEPFEIETAGLWERAVETTIAGVKTRALDSED
                     FLLHLALHFTYQHRLRGGLRNLYDIAEVLEQRMARMNWQKLLATAQQWGAQRVTWLTF
                     QLVEGITRVTAPKEVMTALLPEGADSSAVGAALEQAGSLDDAWAGLTPDLAAFSAAGF
                     FGKIKLLIQRVFISHRVLAREYNINPASPLIMVYYLARVRDLWRRYARSGWRMVAGEE
                     EALAGADFEQQNTRLREWMRGTGKF"
     gene            complement(13840..14979)
                     /locus_tag="P5516_03915"
     CDS             complement(13840..14979)
                     /locus_tag="P5516_03915"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRT91616.1"
                     /translation="MNWIVPLADVKFGVEEQEAVRAVLESGWLTMGEVTAQFEADFKA
                     YTGARHAFAVSNATVGLHMACLALGIGPGDEVIVPSLTFVATANAVRYTGAQPIFADI
                     TSENDLTISLDAIKQAVTPRTKALMLMHYAGYACDMPAILQWAQEQGLMVIEDAAHAV
                     GSFLQEKHLGTWGNAGVFSFFSNKNLSTGEGGMVVTDDDALAAQFKLLRSHGMTSLTW
                     DRHQGHAWSYDVVTTGYNYRIDEIRSALGRVQLEKLDGNNARRSALSARYRELLAELC
                     PALGLPFEGHRGISAHHIMPVLLPRGAQRVRFMERLKEQGIQTSIHYPPVHTFSAYRQ
                     TATGAFNLPITDEVAAREVTLPLYPTMTEDQVQLVTLAAARALEC"
     gene            complement(14983..15711)
                     /locus_tag="P5516_03920"
     CDS             complement(14983..15711)
                     /locus_tag="P5516_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate nucleotidyltransferase"
                     /protein_id="HRT91617.1"
                     /translation="MSSKNAQVHAVVLAGGKGTRLAPYTRIIPKPLMPIGDMPILEIM
                     LRQMKRAGIRQVTLTTGYLDNLLRLFFLDGRRLGLKIDYVREEKPLGTSGPLANVKGL
                     TRTFLVTNGDVLTDLDFAELIAYHQQQGAIATIATHKRKVTINLGVVDVDKDNTVTDY
                     LEKPDFNYLVSMGLYVFEPRVLNYIAKDEYLDFPDLVRSLIAAGEKVSSFTFKGYWED
                     LGRPDDYERASADFERNRSQFLPE"
     gene            complement(15701..16696)
                     /locus_tag="P5516_03925"
     CDS             complement(15701..16696)
                     /locus_tag="P5516_03925"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT91618.1"
                     /translation="MPEHWQRKKVLVTGAGGFIGSHLVGALVERGAEVRTFVRYNSRG
                     DPGLLRMLDARKTESIELVAGDLRDEDAVRAAVKGCEVVFHLGALISIPYSYKHPAEV
                     VSTNIMGTLNVLLACRDSGVERLVHTSTSEVYGTARVAPIGEDHPLQGQSPYSASKIG
                     ADKLAESFSCAYDLPLVTVRPFNTYGPGQSGRAVIPTIITQALCRDVVQLGNLEATRD
                     FTFIEDTVAGFIQAAENQNSLGEVFNLGTGEEIAIGELARRIIKIIGSPAAIETKTLR
                     LRPRKSEVMRLVSDNTRARELLGWQPRVSLEDGLLLTIDWVKKNLHQYRVGQYEF"
     gene            complement(16689..>17070)
                     /locus_tag="P5516_03930"
     CDS             complement(16689..>17070)
                     /locus_tag="P5516_03930"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91619.1"
                     /translation="SSLIGERVLIVGAGDAAQLAIWLMEKSSLYSAFSIVGLVDDDFR
                     KLKKNINGYPVLGTTREIPEIVKQKSVGLVLLAITRLSDAERERILVTCKELQVRVVA
                     IPDLLKVVNDYLSKQTQEVMENNA"
     assembly_gap    17071..17170
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<17171..17979)
                     /locus_tag="P5516_03935"
     CDS             complement(<17171..17979)
                     /locus_tag="P5516_03935"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRT91620.1"
                     /translation="MRFLKRLVDLLGAAFGLLITSPLFLYIAIRIKRESPGPVYYHGE
                     RVGRYGKPFKILKFRTMYETPQKNSGLRLTVKNDERVTPFGAWLRETKMNELPNFWNV
                     LVGEMSLVGPRPEDPEFVDNWPEEVKTEVLSVRPGMTSPASVIFRDEENMLVGSSVLD
                     DYLISILPEKLRLDQLYVRHFNFFGDIDVILMTIVMLIPGLRQRKVKEGTLFEGPLVR
                     FGRAYISWFMIDSIIAFLAISIATLLWRIQIPLDVGFGRMLLLAVGLAFILG"
     gene            complement(18232..18780)
                     /locus_tag="P5516_03940"
     CDS             complement(18232..18780)
                     /locus_tag="P5516_03940"
                     /inference="COORDINATES: protein motif:HMM:NF014417.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HRT91621.1"
                     /translation="MTISWYVMRSKPNREEFLARELESRNVEYYYPRLRVNPVNPRAR
                     RFKPYFPGYLFIHLDLEQVNVLSLEHVPGAASLVSFGGEFSHVPDNILHAIRRKVDAM
                     NEKGGQSAADLHAGDQVEIESGPFAGYMAILDATLPGSERVRVLLQMLERRELPVEMP
                     AHFVKVRHSSSGKKRIPRLPES"
     gene            complement(19042..20004)
                     /locus_tag="P5516_03945"
     CDS             complement(19042..20004)
                     /locus_tag="P5516_03945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT91622.1"
                     /translation="MVKTPNRTQPKISVLIPCFNEEQTIAKVINDFRRELPHADIYVF
                     DNNSQDATASLAAENGAIVVKEKRQGKGYVINSMFTDVVSDIYVMVDGDDTYPASSVH
                     ELIAPVVNEEADMVVGQRLSSYTEGAFPPLHVLGNKLVCSLVNSVFSTNLKDPMSGYR
                     AFNRKVTMQCPVVATGFDVETEMTLQLLYRHLKITEVEINYRERPTGSYSKLRTFSDG
                     LKVINKIFSIIPSYKPLTFFGGISLGLAVTSLFVGSFVIREYIRYQYIYSVPKAVLAT
                     GLMIFAGILLAVGIILHVLNFRLLEESNNNQKMFSLLDRALRDK"
     gene            complement(20277..21017)
                     /locus_tag="P5516_03950"
     CDS             complement(20277..21017)
                     /locus_tag="P5516_03950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT91623.1"
                     /translation="MTQKKDRRITQTEDAFIRDDLEQMKFAQNYNRWLYSLVEPFIGQ
                     RVLEIGPGIGNMTGLMLNHVELLVGMEPNPYCVDVLKDLMPKSSNYTILHTKIEDGDT
                     AALKSYRFDTILCMNVLEHIENDVSVLKTFEEILSTGGKVVLLVPAMPVAFGPIDQAV
                     GHFRRYTARTMKKALAETNLKLQTIFYTNFLGFWGWLFNAKVRRSVRQNEGQIQLFDS
                     LVPILSRLEKIIHPPIGLSLITVAIKEE"
     gene            21549..23159
                     /locus_tag="P5516_03955"
     CDS             21549..23159
                     /locus_tag="P5516_03955"
                     /inference="COORDINATES: protein motif:HMM:NF012711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HRT91624.1"
                     /translation="MKKVENIAYAGLTRFIENGEGEKELLLWVENDLSKTSFSYSYFE
                     TMSNRIAGILQEIGVNPGDTVSLFIPRSPMLISCFFAVLKNAAISSILFSTLGEAGLL
                     DRLSNSNARVIITKKSLLRKIISIKHQLPTLERILVVDIDEDQNDHVLSLKRLLSKSS
                     SAFDYPHSLDAETPAFLQYTSGSTGKPKGALHVHGAIADIEKSFKEIMQLQADDLYWC
                     TADPAWITGLVYGIIAPFTTAARQLQYNGTYNADNWLKLLEQEKVTVWYTAPTALRML
                     MQEEESKFSQRDFQHLKRMYSVGEPLNPEIYHWGKRIFGVEVYDNWWQSETGSMMIAN
                     RPPMKVKPGSMGKPREGIESFICDENMRLLPPGKQGLLCLHKGWGSMFRGYFHNDTAY
                     AEKFHGDIYVAGDLAFTDSDGYFWYVSRADDVINTAGHLVGPFEVESALLEIPEIADV
                     AVIGADDPIMHKRIVAFVKLRSGTAWSKELELKCRVHVSNKVSTSAIPAEFHMIEQVP
                     KNQSGKILRRVLKAVYEGKDPGDTSTME"
     gene            23163..23402
                     /locus_tag="P5516_03960"
     CDS             23163..23402
                     /locus_tag="P5516_03960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRT91625.1"
                     /translation="MNIQERLTKVFRAVFDNESITLTPDLTADDIDEWDSLSHINLML
                     AIELEFGIEFLQSEIQDFRNVGELIECVKTKLEPS"
     gene            23523..25193
                     /locus_tag="P5516_03965"
     CDS             23523..25193
                     /locus_tag="P5516_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91626.1"
                     /translation="MLKYLKISFSFIRRYGGIFLLFIPGVVILIHIFGITLSRIPGDL
                     GDSRMVWYMLEHFYAWISGKIDSYWNAGFFYPYPSTAAFSETMLGSAPLYALFRFLGF
                     SKYLSYQFWLILGFMLNYFTAAFILVKCSLKPLAAGAGAFFFAFGMPIFGQEYHLQLI
                     LRFLVPVAGYLLWRFSQKPRFVLLPLLFFAVVWQFYISVYTGFFLAIMLLVMLFLIPF
                     INGGDTSRNPFRFWSSALVLSFKSTSIPKRILHLFLMAASLFLLAKLFMPYYVVTQEY
                     GFKRSIDYIITMMPRLESFFLADGSQIWNTKTWITKFISHRHEQQLFPGLSVYLLIAA
                     GLVWNFRHANKRFALVNLFTVVVILLLTTYNNGFSPYVYLLNLPGFNSIRTVCRIHLV
                     SMWPVAVFVATVLDALLSAPVKKINFRWVSFLLIGFMLMESLYFDHPTYEKTQAAERL
                     SVLYNQIEKKGLIVKEDSVLYVGEVPGDIPYATDVDGMWLGLELGIPTLNGMTSNFPD
                     GAVVASSDCGRIDSFIRSYMKFASIKEAQFRDQLFKRLIILNMDHCNY"
     gene            complement(25341..27011)
                     /locus_tag="P5516_03970"
     CDS             complement(25341..27011)
                     /locus_tag="P5516_03970"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT91627.1"
                     /translation="MANRFIVKQKIRKANISYRWFKKLQDNAPLPIFTGSPVLPAVTF
                     FLAVRKGTENLADRTIDSLQNLASPYWQVVPVLEDSPIPIGQSAERSADPRIKPAVQW
                     NEFALGQFITDQDGASVFCDSGDIFDKSLLNHYFAIVGSGVKKDIIYYDCEYLSSVGG
                     PKPFFKPSALTPELLISNNYFSRGVFRNASMAAVKVKIEEFSNLRALEYALALKLAAR
                     HCSIQHIPAVLVSIPEERDASIDALQPVLKNHYTGLGFRDVEIQHDGNGRKINWQHGN
                     PSAAIIILSRNHGNWLRTLVQSIQTRTDYANYSIVIVDNQSTETELLNYYQELEDNPQ
                     VKVVPYPQEFNYSTAINLGVSSTSAEIVVLLNNDMQVIEPSWLNELVQWASHPEIGVV
                     GARLLYPNRTIQHAGFIIGMNNFSGHLYLNAPENYFGLTGSVMWYRNFHAVTGACQAM
                     CRTLFEQLGGYDERYRLVFGDVHFCLRAENAGFRNLYNPYATLIHFEGGSRGVETPRD
                     DIQLGCEEIIDNFEGGDPFFASHLSYTNIPYCLLPKKGNPITRKEERA"
     gene            complement(27263..28342)
                     /locus_tag="P5516_03975"
     CDS             complement(27263..28342)
                     /locus_tag="P5516_03975"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT91628.1"
                     /translation="MTQERTLPKVLFIARNYPPIIGGLENNAYNFYQHMSAITETHLL
                     ANPQGKSRLVQFFIETFFYLIFHSSRFDIIHFNDAVMAPFIPVIRLFSRAKITFIING
                     LDIVYHHPLYQLIIPFFLKRADLLFPVSLNTKRECEKRGINPQRLQHIPNGINLKDLP
                     IIESQSAQEFLTKHSINSLHKKILVSVGRLVERKGNAWFLQEVFPLLPEDYIYIIAGN
                     GPQRQRILEIIEQKEWSDRVYLVGWITEDEKLAMFRCADLFIMPNISIKNDLEGFGIV
                     ILEAGYTNLPVVATDVDGIPSAVIPGATGILVPEGDATSFADAILNIHLDREKISLVV
                     DECYNWTKIADQYLQAFVRLTQTTN"
     gene            complement(28339..29310)
                     /locus_tag="P5516_03980"
     CDS             complement(28339..29310)
                     /locus_tag="P5516_03980"
                     /inference="COORDINATES: protein motif:HMM:NF012745.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91629.1"
                     /translation="MRMIIFIPAFNEEGRITETVANIPRMYDGVTDVSVMVVDDGSTD
                     KTAELASVAGAEVVSHTRNRGVGAAFHTAVQYALQHNCDILVGIDADGQFNPLEIPTL
                     IAPLLKGKADMVIGNRFLSGKPANMPGIKFIGNKMVSRFISKISGQVFTDVSCGFRAY
                     GREALLRFNIYGEFTYTHETILSSVYQGLKVVEIPIAVTYYTDRKSRVAGSISKYAAR
                     TSQIILRVLLDYRPLRVFGVIGGGFLAIGMGFGLFLFFHYILTGSFTPYKSFGFIALG
                     FGLFGLIILVIALVADMLNRMKTNQDKILYEIRSLRYQNTTPKDKPQ"
     gene            complement(29600..30703)
                     /locus_tag="P5516_03985"
     CDS             complement(29600..30703)
                     /locus_tag="P5516_03985"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT91630.1"
                     /translation="MRRTPTISIFLNSYNHEKFLRESIDSTLGQTFTDYELIIKDDAS
                     TDSSWDIICSYSDSRITAFRNEVNRQRQFGHFLPDLRGQYVAMHNSDDVWEPTKLEKQ
                     VAYLEAHPEVGAVFTRVVFIDENSRPISGNISFPINFFNQPNRDRFGWLNYFFYYGNA
                     LCHPSVLIRKECYEKCGCYRRGLVQLPDFDMWVRLCLKYEIHVLQEKLVNYRLLLDSA
                     NASSPTLINLIRSNFEKLQIYQNYLALTNRDELVRVLPAIIRYEVGEYYNHRYLIARA
                     ALDKEDDPVCWLFGLQVLFDLLNDPREAAALKQYYQFTPNTFSQLSGSRDVFHEEVHQ
                     QEYQLVHFIKRKIWKWIKPLRDIENLLKKIKKN"
     gene            complement(30730..31299)
                     /locus_tag="P5516_03990"
     CDS             complement(30730..31299)
                     /locus_tag="P5516_03990"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRT91631.1"
                     /translation="MAKEKIIIENTSPNRVYLRAVEPEDYLTSYRWRSNFEALQYETH
                     RYVSKETERKWVLKIIEEHETGKSVRLAICLKENNQLIGYIHLSDIDYQNRSCEIHTL
                     IGSKEHQGGGFGTEARIMILRYAFLELGMERVRSRIIANNAGSIKSGIKSGYTYEGTL
                     RKAVFQDGQFFDVLLFSILKEEFLQKSNG"
     gene            complement(31467..31922)
                     /locus_tag="P5516_03995"
     CDS             complement(31467..31922)
                     /locus_tag="P5516_03995"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT91632.1"
                     /translation="MKNVTEMNRKDRVYLRAIEPDDYLVSQKWRNDFDLIKGYTAPRY
                     VSLEMERKWVQKVIEENESGRSVRVGICTVGEDRLIGYANLLNIDYKNRNCEFSIIIG
                     DRNYHGKGIPAQVRIILFQHAFLNLGMERVWAKILSTNIPAITNGERFG"
     gene            complement(31981..32523)
                     /locus_tag="P5516_04000"
     CDS             complement(31981..32523)
                     /locus_tag="P5516_04000"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRT91633.1"
                     /translation="MDIYLRAIEPDDYLVSQKWRNDFDLIKGYTAPRYVSLETERKWV
                     QKVIEENESGRSVRVGICTVGEDRLIGYANLLNIDYKNRNCEFSIIIGDRDYHGKGIP
                     AQVRIILFRHAFFNLGMERISAFVLSTNITAIIEGERFGYVKEGVLRKAELQDGQLID
                     VNIYSILKEEFIEKYARDLE"
     gene            complement(32626..33621)
                     /locus_tag="P5516_04005"
     CDS             complement(32626..33621)
                     /locus_tag="P5516_04005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91634.1"
                     /translation="MVKVDRAPQRHLLKPLGGYLGLELRDGEEYHTNAIRVNTGANAF
                     KTILLTGKYQKIYLPIYNCDSVLQPLKELQIPYQFYPVDHHLDPVFEIGSLKEGEGFL
                     IVNYFGLKDDSIAALRKQTGNVIVDNAQSFFSKPIQDTDTFYSTRKFFGVPDGAYLYL
                     KTKVNLALEIDHSYHRAEHLLRRIDVSAEDGFAFYRQNEQHLSSQPLRTMSGLTRALL
                     KNIDYEHIRRVRVDNFGYYERALKNHNLLKVPWNGTQVPLIYPFLTDDVHLYDRLLDE
                     KIYTARYWPGVRELAEPGSWEHQLARMLIALPVDQRLSQTDIERILKIILKSHRR"
     gene            complement(33665..34576)
                     /locus_tag="P5516_04010"
     CDS             complement(33665..34576)
                     /locus_tag="P5516_04010"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT91635.1"
                     /translation="MAEGYLHPSYADSFSEVGTALFLPRSKGWLIKRPVPGSGYFDAM
                     GCYPLFLCENWNLLREDIHDLNHELVSVTLVTDPFMQGSFEQLSETFPLCYHYKDHYI
                     TDLSLPLEQTISRKTRKLAERALREIVVERCHEPAQFLDEWVDLYTVLINRHNITGIR
                     AFSRNSFSQLLSLPGLDLFLARLAGEIVGAEIVMRQGSIGYGHLLAISSTGYEHNASY
                     ALDWSILKYYADTLRFFDQGSGAGLENAENGLVQYKKRWTTLIKPVYFCGRVLNEGVY
                     QELCDQKNAAGSNYFPLYRLGEFNPGG"
     gene            complement(34601..35674)
                     /locus_tag="P5516_04015"
     CDS             complement(34601..35674)
                     /locus_tag="P5516_04015"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013227.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRT91636.1"
                     /translation="MIPVTQSYLPPMEEYLHYLNQIWESHQLTNHGPLVLELERKLKE
                     YLGVRHLFFVSNGTIALQIAIRALGLRGEIISTPFTYVATTSSIVWEGCTPVFADIHP
                     RTLCIDPQLVEESITPQTTAILATHVYGIPCDVAELQRIAMKHNLKLIYDAAHTFGAR
                     YKGYSLASYGDVSTLSFHATKLFHTAEGGAIITNNDQVAHQISYMRNFGHKGQEDFWG
                     LGINGKNSEFHAAMGLCILPHLAECIAARKQVCGWYDERLAGSALTRPLIPEGSEYNY
                     AYYPVLFPAEKSLLRVREALNDRQIFPRRYFYPSLTSLPYVSTKRCPRSDEISPRVLC
                     LPLYTGLSENEVALITETILASL"
     gene            35875..>36884
                     /locus_tag="P5516_04020"
     CDS             35875..>36884
                     /locus_tag="P5516_04020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91637.1"
                     /translation="MFFFSVFFYVAIALLLVLRHSNELRASRFIISLFITISALNILV
                     IQTLHFLRLLDNPLTYLFVQVFLCALCALLLIDPKKKLFKTPLNWPQVSCSKISWVEG
                     ILFFFLCIVLAGVFYVGTLSPINNSDSLQTHLPRMFYWLQHRSLISWYPTAATQLSYP
                     VNITFLGLWAFLLSGSEMYLFVPMWLSLVAIAALVFEIALLLGAQREAAITASLVAIS
                     LPVVLMQTYSYQGDAFVAAQVLCVIFFLLLYSTQKKNTDLVLSALVMAIALGSKQTAI
                     LFLPIYLLSLLLLILGKTLNFRSFLNTILLAILFFGVFSSLKFVQNLTETQTDAAHMV
                     NPG"
BASE COUNT         8889 a         9643 c         8725 g         9527 t
ORIGIN      
        1 cagaacaacc ccaaaacaag cgctggagat ggcttgacag actttgccag ggcgatgcag
       61 gaacctggca ttgggatcat ctgtgccaat gcccctcaag ccaagggcag agtagaaagg
      121 gccaacaaga cactgcaaga caggctgacc aaagaacgaa aactgctaac tatttcaaca
      181 tgcatgcagc caacctttgg ctgcccaagt tcatggaaga ttacaaccag cgctttgcca
      241 ctgtaccaag aagtgagcac gacttccatg aacccctgac ggattcaaac atcctgtgct
      301 atatccacaa tcgtaaagtg acgcgcaccc tctccagaaa ccgaactttg cggcggcaca
      361 agaggattta tcaaatccaa gtcgatcgac ctggctcagc agaatgcctc caaagcccac
      421 gtccctgtcc cagaccatcc ttggagaaaa ttcagcctta acaaaaatgc acccagaaag
      481 gtgacatctt aacttagtca gatgaggtga gattaaactt caggctaaca gtggttttaa
      541 gaaagtggtt tttatccctc tcttttcctg aaaaattaaa gtgactgcac cgcgcgccca
      601 tcctgcccgg tcagagaatt aataaaaaac ctcccggaat tgcccgggag gtaggatact
      661 cctgcttttc ttttatggct gcttgttgaa aacccgcacc tcaaccgggc cgttcagctc
      721 tgtgtcgccg cgcacgctca cagtttcaag ccaatagtag tacgtgcgcc cgttcttggc
      781 ctggtgcaaa accgtcccac cgccccctcc gttgccaggt ttgaccgtaa aaccagtgtc
      841 cacgtagata tagagagagc ctaccatgct gcccggcggc tgcttgctgg gaatcatcaa
      901 gtcattcact ttgacacgtt tcccattcac aaaattggcg cggtaaatat taaacccgag
      961 attatccaac tcgttggtcg tttcccagcg tagagtgacg gaacgtaccc cgtctctggc
     1021 agtgaagttc gttaaatcag ccgaagtggg gtcacaggcg ttggtgtaaa gcatgccatc
     1081 cgcagttgtg taaggcaaat tgtgtatgct ggaatccatt agctctgtcc cggaagtgat
     1141 ggtcaacctc acatttttat tcagctggtc aggttttggg cgcatcctga tcgtaagcag
     1201 cgttcccgcg ccgctcttgg gggtttgcgg attgatttgt gacatcgcaa acttaattgt
     1261 cccagcgtca ttatcccaga tcctgtgacc gtaaattccg tcctgtaaaa aactaccgtt
     1321 tttcacttcc aggatattca gtgccttcgg gtcaaacgat agctgcagat catacgccca
     1381 caggttgaca gcgtaacgga cgttgacatt gacgatggta tcccctccga tttggcagac
     1441 gagcgtttca gccgggttga tggttagcgc cactggcggg caatcatcag agaggatgac
     1501 cgtgccgttg aaaattctat tcgaaggaat atcgatagac tgtccgtcgc ctgttgccaa
     1561 tttttgatac gtaatagtga tgggcaccgt ttccccggtc gttaaagccc gcacccggat
     1621 gtaaatcagg tctccgtcgc ctgttttatg ggtagatggc gccagttggg tatacgcgac
     1681 ccaaacctgg acgttccctg agatcacatc cacttgattg cgaatgtaat accctgtgtt
     1741 gtccaaaaaa gtggcgttgg acactccgac cacctcgagt ttgcccacag ggaacgacac
     1801 tttgacatca tacccataca gacccggggc gtagatatcg gtcactctga tagcgatgac
     1861 agattcctca cagttcagca ccgtctggct ggcaggagaa acgaacatac tgggaggtgc
     1921 cgcgctgacc ggtgtgatat tgaccgaagc gataacgaat gtgatcacga taaacgtgat
     1981 caggccttta aggaacaggt tgtttttatt cgtgattttc ctccactttt ttgcgcgttt
     2041 tctttttaag cataaaggtt actccacccg ccagcagcac gataaagacg gctatcagcg
     2101 gccagccatt ggccaaccct gagcccttcc cggctgccgc cggcctgcca ggctcactgc
     2161 caccggttgc agtagaacct ccatttccag tctggttttc gccccctggg gccgcttgtg
     2221 gttcaggcgt tcgggtcggt atcgtggttg ccgtgaccgt cggtgtgggc gtgtctttgg
     2281 gcaagatgat caatcccgtc gcctgggcaa cggcaatggt ggctgcctgg gtcgctgccc
     2341 ccgagatgac ctggatgttg gaatcaagca ctttcgcctg aatggcggtg atctcgccgc
     2401 tcaacagctc aacgccagtg aagtgaatgt tcgattcgcc cgtagagatc cctttaaatg
     2461 taagcaccac gatattgccc tcgcccgatt tcggcaggct ggggttgtac tgtgacataa
     2521 caaaacggta ttcacctttc tggttatccg cggtgttgaa caccagcaag ccggggtcca
     2581 aaaaagtccc caaccccgcc tgaatacccg gctggatggc gtctgcgtcc acaagctgca
     2641 caattgtggg gtcatattcc agcgaaaaat ccacagcgta cagcccttct acatccttca
     2701 ccgccactgg cacctgcacg gtcgctccgg gcgccacgac agcaggttcc atcaccccaa
     2761 acagcgtctc taacttctgc gcccgggcat tggtgaagcc catcagtagc agcataacaa
     2821 agcatgcctg gaagatcgtt tttgtaataa acttatttga aacagaatta ttcttcttat
     2881 acaaggtcac atctttctcc aaaggttgaa gaataacacg gtcaaacccg tgataaccgc
     2941 cagaagcaag aatcccattc cccacaaagc cccgttggcg ttccgggttt tgtgaaggaa
     3001 ttcatccagc cgggtggcat ttttccattc agccgtcaag tgtgcgtatt cagtcgaacg
     3061 tttcatttca gtggtctgct gcaacgcgcc atccgttacg gttccattgg acagcacatt
     3121 ttctggtata cccgccgcgc caccacccgt gatcgcgtct tccgggccag taacaattga
     3181 gaagggggtg gcagtggtca cctgtccagc aggcggcacc acaaccgccg tccccggggt
     3241 gccggtgcgc gttatcacaa tggcgtagat cggcgtgatg gtcctgccat aaatcgcggt
     3301 aggggtgcgt gtgcgcgtgg gcgtccaggt gatgaagggg ttcatggttg cagaagcggt
     3361 cggggtgtga gtgctggtcg cgctggctgt attcgtggtc gtgtgggtcg cggttgcggt
     3421 ataaatgctg ccttgcgtgc tcacctccac cacgccattt ctggccgttg gatagatcct
     3481 ttggttggca atgtttacca gctgcgcatc ctgtatttca atggtggtgt gcccaaggct
     3541 cttccctcta aacctcaggt tgagcagcgt gccattgccc gtagccccgg gagatgccag
     3601 ctgcgtcacg gcaagccgca acgaaccggg cttttcatcc ctgtagacga cagctacgtt
     3661 ggaaagaaaa acaccatgcg accaggattc cagcgtcagc acctccttat cataaacgac
     3721 tttcaagtcg tatgcgttta aatcaaccgc gttggcaaca ttgaccttaa gctccaccaa
     3781 tgcgccaaca gtgatgttgt tctcgacggg ttcaatactg acctcggtat ccccttgcgc
     3841 ctgggcagta aattccccgg tatcagcaac gcccaacaga aaagacagcg ttatggtcat
     3901 cacgaataag atacgaataa tgcgcttact catgtcctct ctattctatc ctatcaagga
     3961 catttccaat aaatatcacc gggcaacagc cttacaatct ctttaagcgc tgttttgttg
     4021 gcttgcctga atggtaaagt gctgttcgtc tgcgcataac cgctgcgcgt gcatgctgca
     4081 ggccggacag gccggcctgc agcatcagtc gatttgttta cggcatccag gtgtaattga
     4141 ccgcggagct cttcccgtag ttcgaaccaa caatggcgag gtctgagaga cccactttca
     4201 gtgagaagtc cacatccgca tcggtgtctc cctgagtgga tacgtcccaa ccatacttat
     4261 tgcccaacag gctggcatcc acaatgtcga ttacgttatc cgaccagttg gcattaccgg
     4321 cgatcagggt gagcggggtt gagaaggtga cgttgctgct cacaacagtg atggttttcg
     4381 ccaggtcttc cgtgatgttc aagtagcgtg gttgattggt ggtgaatgtg taggttcccg
     4441 gcgccacgtt caagaacttc atgttgtacg ggatcggctg aacagagttc gccatataca
     4501 ggccataggt gccaccggtc aatgttcccg gaattccgga cctgacattc atataacgat
     4561 attccatggt caaagttccc tggacggtgt aactgttggt ggtaaccagc gcgtcgagga
     4621 accccatatg atagatattg gtggagtaag cggggttcca ggttgggtct gccgttaccg
     4681 gagccatggt aaattccgcg cccgcttcta ccaggtcaag cagagcatca tcacccaata
     4741 cagtgaaggt cactgaatag atgggcaggc tcttgccaga aatgggagtc gccgcgggga
     4801 ccttgccagt aaagcggatc tcgcccgctg tcgaggtgtc aaagacaccc gcgcccggag
     4861 caacctcaac aaagccgctc aggttggtca gcgaagccgg gacaagttga agtttggccg
     4921 cggggtaggc cagggtgaac ccggcgccca tcacattagc gaggttgccg tacactgtgt
     4981 aagtcacctg accaccaatt gaagccgggc tggtggagta cagatagagg tcaacgtgcg
     5041 tccacgggtc ggcatctaca ccggtgctca cgccatctcc tgccaccggt ccatcgatgt
     5101 caccccaggc gttgaattca gcggggatga tggcgccata ggattgtatg cccctgccat
     5161 cattctcctg gaagaggtta ccctggatgc tgattatccc ggtaggctga tcctggaact
     5221 caatcgcgtc accggtaagg ccatggaacc agttatccag gatggtcagg tagtccacca
     5281 ctccggagaa cagaactcca ctgaccccgc ctgcgctgcc gtcaaattca aggttggcga
     5341 tgttcacgtc ttgcagtcct gccgccacgt tcaccccatt tttgccggca gccggcacgc
     5401 actttgcgcc cagtttgctt tcagccatga tagagatgtg gtcagcgaac acattgaagc
     5461 aggcttcatc ggtgttctgg atggtggtgc cgtcaagaag aagataggtg atacccggca
     5521 cgtacaagcc attcgtctcc aggttgactg taccggaaag cgacaggttg ttcccattct
     5581 gcggcatcag cggcaagcaa gttgggttgg ccgtaccaca ccactggatc acatccacgt
     5641 tgtcagtgat gggcgcttgt ggtccaagga ggctgcccca ccagttctct ttaagattcg
     5701 ccgcatagat gccttgcgcg ccctggtata cggcatattg ggtgcttcca acaatgttgt
     5761 tacgcttgat atcactggtg atcgtctttg ccacgtatgg cgaaggttcc catgaagcag
     5821 gaccagcgct caagacaaca cctgatgcaa aacccgtgaa ggtattacca gtgaagtttg
     5881 cgttcacatc gtagtaaccc cagcctaatc cgctagtggt gatataagcg ccaacgctat
     5941 tgttgttggg tactggcgct gtggctgagt tattttggaa agttgccaca gaagtgttac
     6001 ccgaacccca ggcagagtag gcaaccccgc agcccgttac agtattgttt tcaactgtga
     6061 ttggcatacg gttgacaaaa gtccagatgc cataaccgct ggcttggttg tcgcagtcca
     6121 cattgtttcc actgatcact tcagggccca ggatgttcgg gaaacctgca ttatccgtat
     6181 ggataccgcc aggggtgtgg gtgagggtat ttctgagcca ttgagtacct tgagagtaat
     6241 tcgctccaac tgccttacca tagtcgatga cgttgtcagc gacagttcct gtgccgttat
     6301 agaccatgat ggcaaatgac tgtccagtcc cttgagtgtt gctgacattg ttgtgatgga
     6361 tattgaatgt tgcaccgctg gaagcgctgg aagcgtagat tccgcggaag tagatgttct
     6421 tgactgtgac atatgaaact tccaacccgg tgaacactcc agccgtgtgg tcggtgatga
     6481 tgccgttgcg cgcatccagg tccgcgccgc ccacgacgac ccctgatacc aaggcggggt
     6541 tatctccgtc aatcaccagg tctgtgatct tgacgttatg ggctttcacc aggaacacgt
     6601 tgctcccgcc caatgagcca gggagaccaa cacccgggtt tgaaacagcc ggcatcacaa
     6661 tcgtgcccgc tccagcgcca tcgatggtca ctgatttgtt cacgaccaca ttctccacgt
     6721 acgtgccggc ggggacgtcc accgtatcgc cggttccagt atagacatca tcgatcgcac
     6781 tctggatgga gctgaagaaa gtggtttcgc gatcagtcac ggatggaatg gtggtttctt
     6841 cgatgttccc taggctatct cgcacaacca ccactttttc ccagtcgttg tttgcgagcg
     6901 tggcttcgag gtcaatcgtg ccgccagtat gagccaattt cttgaacata taggcgtttg
     6961 gcgcgttggt tgcgcccaat tgcacggtgt tatcctcaaa ggcaacattt ccagcgtaga
     7021 aggcttgcac caataagcca tcccattcag ttaccgggtc agtagctggc gctggaatgc
     7081 ccactgattt gttccccttg atcagccaat tgacctcagg ggcttctgag taattgaacc
     7141 agatgccagt tctgtaaccg cgtacttcgt tattctcaac aaggccctgg gcggtttcag
     7201 ggtttccgta gggttgaatc tggatacctc ggtatgcctt atccacaaca ttgccgcgga
     7261 caacgccatt gtcgccctgg acgtaaatgg cagagtagtt gtagtaacgt ccaggcacct
     7321 cattggtcac aaggttattc tcaatcagca gcccggtgtt gtaaaaagtc cggtcccaac
     7381 tgccacccac gaactcgtac gaggaggaga gaatcccgat atagttgaaa tttttaatga
     7441 tattgttctt gacggttaga ttcttttcct ccgagacgat gcccgcgata tccacatctt
     7501 ccgctaaagc ctgtccatca agcgtgaagc catctacttt aacgttttcg gcatcaatga
     7561 agatcatttc ctggtaatcc gttaagcccg ccgggaagac aatcaccgct tcagggacgc
     7621 gcacgcctgg tgcgacgcca gcggatacgc catagttcgg gccgcgcagg tcgatcgatt
     7681 tttctatact cagctgttcc tcgtacgtcc ctgcctctac agtgatcaca tcgccatcca
     7741 cggtcgccgc gtcatcaatc gcagcctgga tggagccaaa gtaggtatct tgggtcacgt
     7801 tatgaaccgg gccatagaac gctgtgcatt ccggattggt gcaccagggg gcgtatttga
     7861 ggttgctcac aacataccca gctgcaggac ctgacgcgct gccccaccag ttcggtgacg
     7921 cgttctggtc gcttgccgta tcctgctcct catacccaat gtcgttatcc gctaactggt
     7981 tcttttcgct ggtgataccc gtcgggtata ccggttgacc ccaagtgcca gatagccaaa
     8041 tgccgcgatg agcgttggta acgatgtttt ttcgcagggt ggtatcgctt accggcccaa
     8101 gaagcctgat tccctttgag gaagaaacgc caggggttgc ggttagtgtg ttattttgaa
     8161 tcacaccgtt gaaagtgctg ttcgtgccat ccacttccac ctggattccc acgctttgga
     8221 acccttcgac cttgtttttc tcgataaggg cattggtgta ggagccatac tgacgcacat
     8281 tccaacccga cccccacgcc ggggcaattg taatccccga ccaaatggag ccccccgctg
     8341 ctttggggtg aatgtagttg tcatggtacc aatgcaaccc cgtgaccgtg tttggtacca
     8401 aggaagaacc gtaggccata ctccatatgg caggtccacc gtcatttggc accaagaatt
     8461 cattgtcccc aatctcaacg gcaccagtgg aattttccac catgatggaa ttaccaccaa
     8521 tattatcaaa caggttgtcg ttgatggtaa aggagcaggt accgtgctgc gccagcaatc
     8581 cccaatcttt atcagtatca cccgtgccca agaccgtgtt atcggtgaag ctgactgcag
     8641 cagggctttg gcacccgccg aaagaaacaa ggtaagtcat cgccgcgttt ggatccgggt
     8701 catccttgaa cgtgaacccg cttatatcca gcacgccact ggtcaacccg gcagaacgaa
     8761 atagtgttgc attgctcagg ccggtcccat ccacaatggt agtctcttta ccggcgccga
     8821 tcaacttgag cgctttattg gcagtaaccg cttcgttgta attccctgcc gccacataca
     8881 cggtgccgcc cggtaccacc gcgtcaatgc ccagctgcat gtacttgaag ggttcattga
     8941 agttccccct ggatttatgc gcagtagcga aactgctgtc atcatcaaca aaaacgtggc
     9001 tgcggttgtc tattgatcca gtggtcatca ggctgatggt atagccgtcg ttgtttgcct
     9061 gcgagacctg taaaacgctt ttattcatat ccatagcgta agggccgcca ccagagttcc
     9121 agacaatatc ccattcattg tggatgccat tccaggaatt accagtcatc accctggcat
     9181 ccgcgtccgc ctgggtgccg gtgtagttca tgatgccgcc cttggtgttg taaatcacat
     9241 tgttgctgat ggtaagagcg gaattgtaat gaaccacaat gccgccgcga gtattgtaaa
     9301 tggtattgcc ggaaacattc gagccggtga tatacctgcc taaatacacc gcctgcccgt
     9361 accaggtgct cattgctcca ggttgcgggt caacataaat tttgttgtcg gtgatattaa
     9421 tattggacct tgggccatca ggcacattga tcatgccaat atgggctgag ggcgcgttgg
     9481 cgagtgtgat ggtcaatttt gaaatggtga catcactgga tgtaatttca aaaactttca
     9541 cagttgaacc acccacaccg tagaccttcg cttcaccgct tgccgggccc aggatggaca
     9601 tagccttatt cacattaatg accgccggag gatagtaact gccatccgcc acatgcaccg
     9661 tgccgcccgg cgtaacccga ttgacacctt cctgtatggt ttggtaagga gcctcctgcc
     9721 caccatttcc gccggcaggc gcatcatcat ccacatagac cacagaaagc aacgcgggtg
     9781 aatgacgctg gtcttcaatg acagccccgt tattcgcgtt gctcatggcc acgatgtcag
     9841 ggtaataagc tgcaggcatt gcggacaaaa tggctacatc gagaacattg cccgcccagg
     9901 tgttattgga gaaggtaagg tttccacctt caagcaccca ccccctggta ttgtgaacgt
     9961 aattatggga aatggttccg ctgtgcgtgc cgctaatgta agccggctgc cgcaggtcgt
    10021 agatctcgtt ttggtcgatc agtagatcgg taaacacccc ggaattaatg atcatcgccc
    10081 gcgacacatc accatcaccg atcacatacc gaccccagat cttgttattc tgtatcgtaa
    10141 tgtcgcttgc ccgaattctg atgatatttt gttcgcctgt tttatcggtt ttttcaattt
    10201 ggcagcctac cagagatgca ccatcggcgc tgaaatcgaa ccgatcgcct gttccagaaa
    10261 cctgaatcaa cgcgcccggg tcgcagctca aggtgacagc tttatcaaca tttaccatgc
    10321 tgctcaacac atgagtgccg taaatattga tcgtatcgcc gtcagttgcc tcgtttatcg
    10381 cgccttgaat tgtgccaagg atcgagttcc cgcctggaat caaagcaacc ggtccaaacg
    10441 tgttcgccgt gaaaatggcg tccatatcaa gtgaggatgt attatcgtag atattgattt
    10501 cattaccggt gagcgtattg cctgaaacag atactgttcc acctcccaca ccgtagacaa
    10561 gaatacctgt cgcttcgtta ttcgtaggtg tgtaatcaaa acctgagatg gtcgctccgg
    10621 agactgacag gtcgatcgac ccgccacctc tgttccacgc catgatgccg ttttgcgcat
    10681 taactgatgt aggacccgca ccggtgaacg agccgccagt gatagagagg gagattgttc
    10741 ccgcgttagg agtggatgcc ttaccatcca caacatcgat cgcgttcttc tgcgcaccgc
    10801 tgatatcggt attgactaac gccagggatg tcgtctcacc agcgcccgcg tcaaccgcca
    10861 atccctgccc ggtttgattg ccgctgatgg tgtaaatacc ctcgatgctg caatccgtaa
    10921 tcgtccccgt cgaggcgttc cagaatacaa tcgcatccgc cccgccggat ccaggtgtcg
    10981 cagaagtgct ttttaccgtc aatccctcaa tggtcacatt gttggattga tttacaaata
    11041 cgctcgccag ggttggcgtt acgtatttgt gtccaacacc tgtgttaatc agagaagttg
    11101 gttcgatcaa ggtctggcct ttcccagcgc cgctgatcgt aatattgctt ttcccaacga
    11161 tactgaaccc ttcgtatgag ccagctctga tggagatcac gtctccactc actgctacat
    11221 tcacagcgtc ctgaatggta ctgtaggtac aaccgctggc gcagacatcc agggtagagg
    11281 ctttcgcttc cctggcagaa acaagcccaa acacgaaccc aagaatgaca acaattacga
    11341 aaatcttaga aatggttttc atgatttctc ccttttagat ggtgatagat gatgagttgt
    11401 gatttgtcgg cttcttttat ccatttccct cctgttttgg atgtctttta tcatgttaaa
    11461 tataaataac cctgcttaca cagttgagag cactatctgt cgttacagca ataacaagcc
    11521 ccattatccc ctttactaga gcatttactc taacaggtac aacaaaaaag tgggttgaat
    11581 ttttttatga ttatttgcct atgaaaaatt atagagcaaa aataaatttt gtcaacattt
    11641 tttctatagg tttcccctgt tttcccccgc tggtagaaaa cttgcctttc tgtcttagag
    11701 cgaaaccctc aaaaggctat tgttcgaaat agataccgca cgcttccggt ttcggcaggt
    11761 tgccttttga gccgcaaaat tgccgtgacc ccgccatatt cccgccaaat tgcccggggc
    11821 catacaactg ccagaagaaa ggagcgctaa cgccgttaaa agaataatcc ccggtcacct
    11881 gctgcccgtc tgctgagatc tctccgctga tggtcatgcg cccatgactg aagtcaccaa
    11941 ctgcattcaa cctcgtgcct gagacggtca cattcacttc gccgatggtc agttcttccc
    12001 agtcagcgcc aaaatacacc gtccatttgc cgttccaccc tgcgagcggg ttggcgctcg
    12061 gcgcaggggt tgcagtggct gttcgattct gcgcagggtg cccagtcgcc gtggcggtga
    12121 aaggcggttg cgtggattgc ggaagaactg aatcgctcaa tggcaagccg gtttgcgcag
    12181 gctgtcccaa tcctccttca gcaactctcc catccgcatt attggctggg tttaaagtgc
    12241 caaacggttt tcctaaagaa ccactggtgg gcacaatgag aggggtttct gtggggaaag
    12301 gaacgcccaa agtgaggtac aactcagcgg tgctggttct ggcaacttca gtctgcttga
    12361 tcaagatatg gagggagatc cgcgtttgct caagggcgat ttctttgctg gtgagcatgt
    12421 gcgccgggcc gttaatgaca aaagttaaca tcacgatgag cgccaggatt acaacagccg
    12481 ccgccaaaac aactccaatc ttcttcagtg tggatttttg gttcatcgtg ggtttaatgt
    12541 atcataaaat tccaaactgg tcatcttaaa acttacctgt ccccctcatc cattccctca
    12601 gacgggtgtt ttgctgctca aagtctgccc cggcaagggc ttcctcctcg cctgcaacca
    12661 tacgccagcc ggagcgcgcg tatctgcgcc acaaatcgcg cacgcgcgcc aggtaataaa
    12721 ccatgatcag cggtgaagcc gggttgatgt tatactcgcg cgccaacacc ctgtgggaga
    12781 tgaacacccg ctggatcagc aatttaatct tgccaaaaaa acccgccgcg gagaacgcag
    12841 ccaggtccgg cgtcaggccc gcccatgcgt catctaaact gcctgcttgt tcaagggcag
    12901 cgcccacagc agaagaatcc gccccttcgg ggagcagcgc ggtcatcact tctttcgggg
    12961 cagtcaccct ggtgattccc tcaacaagct gaaatgtgag ccaggtcacc cgctgcgcgc
    13021 cccattgctg cgctgtagcc agcagtttct gccagttcat cctcgccatg cgctgctcca
    13081 gcacttcggc gatatcataa aggttgcgca gccctccgcg cagcctgtgc tggtaggtaa
    13141 aatgcaacgc cagatgcaaa agaaaatctt ctgagtcgag cgcccgcgtt tttacgccgg
    13201 cgatcgtcgt ctccaccgcg cgctcccata accctgccgt ttcgatctca aaaggttcat
    13261 cctcttccaa tatggaccag tgcagttcga gggtgggatg ctgcgccttt tccagcggcg
    13321 gcaaatgctt gatgtcagtg ttttgcgcct gcgggtcata ccaggtagag agcctgtacc
    13381 cctggttctg catcagggtt aacgcgtccg ccagttgttc ccgacgcacc aacaaatcca
    13441 gatcactgaa ggtgcgcagc ccgatctcgg ggtacacctg ttcaaccagg tacagccctt
    13501 tgagcacgat cacttcgatt ccgcgctctt gaagcgctct aaggatcccc gcggcgtggt
    13561 gcagcatcac catattgcgg gcggtggccg ccaggtaaac ggtctgtaga tgttcccaca
    13621 cccattcggg aacaagcgcc tcgccatgcg caacgcgcag gcgatagtag agcgttaagg
    13681 tcagtgcgcg tttttcggct tcctccagga gggcgcgcca atcttcaggc gcgagcgggg
    13741 atgttttttc cgccagcgtg ccttcccagg ggtaagcgag caggttgatt aaggacatcg
    13801 ttggttgaga gaaagccatc tccatactgc caggcgcgtt caacactcca gcgccctggc
    13861 cgcagccagc gtcaccaact gaacctggtc ctcggtcatt gtgggataga gcggcaaagt
    13921 cacttcgcgc gccgccacct cgtcggtgat tggcaggttg aacgcgccgg ttgccgtttg
    13981 ccgatacgcg ctgaaggtgt gcaccggcgg gtaatggatg ctggtttgaa tgccctgctc
    14041 tttcaagcgc tccataaaac gtacccgctg ggcgccacgc ggcagcagca ccggcatgat
    14101 atgatgcgct gaaattcccc ggtgtccttc aaaaggcaac cccagcgcgg gacacagttc
    14161 cgccagcagt tcgcggtatc gcgcgctcaa cgcgctgcgg cgggcgttgt tgccatccag
    14221 tttctccagc tgcacccttc cgagcgccga acggatttca tcaatacgat agttgtaacc
    14281 ggtggtcacc acgtcgtagc tccaggcgtg cccctggtgg cgatcccagg tgagcgaggt
    14341 cataccgtgc gagcgcagca gcttgaactg cgctgccagc gcgtcatcgt cggtcaccac
    14401 catgccgccc tcaccagtcg aaagattctt gttggaaaag aaactgaaga cgcccgcgtt
    14461 tccccaggta cccagatgtt tttcctgcaa gaaggagccc accgcgtggg cagcgtcttc
    14521 gatcaccatc aacccctgct cttgcgccca ctgaagaatc gccggcatgt cgcaggcgta
    14581 accagcgtaa tgcatgagca tcagcgcctt ggtgcgcggg gttaccgctt gtttaatcgc
    14641 atcaagcgaa atggtcaagt cattttcact ggtgatatcc gcgaagattg gttgcgcgcc
    14701 ggtatagcgc accgcgttgg cggttgccac aaaagttaat gatggaacaa tgacctcatc
    14761 tccagggccg attcccagcg ccaggcaggc catgtgcagc cccacggtgg cgttggaaac
    14821 cgcgaaagcg tgccgggctc ccgtgtaggc tttaaagtca gcctcaaatt gcgcagtcac
    14881 ctcccccatg gtaagccagc cgctttcgag cacggcgcgc accgcttcct gttcttcaac
    14941 gccgaacttc acatccgcca aaggcactat ccagttcatg ttttactccg gcaaaaactg
    15001 gctgcggttt ctttcaaaat ccgcgctggc gcgctcgtaa tcatccggtc gtcccaggtc
    15061 ttcccagtat cctttgaagg tgaaagaact aaccttctct cccgcggcga taaggctgcg
    15121 caccagatca gggaagtcca ggtactcgtc tttggcgata tagttgagca cccgtggttc
    15181 aaagacatac aaacccatgc tcaccaggta attgaaatct ggtttctcga ggtaatcggt
    15241 taccgtgttg tctttatcca catcaaccac gcccaggttg atagtgacct ttcgcttgtg
    15301 agtggcgata gtggcgatgg cgccctgctg ctggtggtaa gcgataagtt ctgcaaaatc
    15361 aaggtccgtg agcacatcgc cattggttac caggaaggtg cgggtgaggc ctttgacgtt
    15421 ggcgagaggg ccggaggtgc ccaggggttt ctcttcccgc acataatcga tcttcaaccc
    15481 caatcgcctg ccgtcgagga agaagagccg cagcagatta tcgaggtaac cggtggtaag
    15541 cgtcacctgg cgaatgccag cgcgcttcat ctgccgcagc atgatctcaa gaatgggcat
    15601 atcaccgatg ggcatgagcg gcttgggaat gatgcgcgtg taaggcgcca ggcgggttcc
    15661 cttaccgcct gccagcacta ccgcgtgtac ctgagcgttt ttagaactca tattgaccta
    15721 cccggtactg gtgaaggttt ttcttcaccc aatcaatggt tagcagcagc ccatcctcaa
    15781 gggacaccct cggctgccat cccaggagtt cccgtgcgcg cgtgttatcc gaaaccaggc
    15841 gcattacctc tgactttcga gggcgcaaac gcaacgtttt ggtttcaatg gcagccgggc
    15901 tgccgatgat cttgatgatc ctgcgcgcca gttcaccgat ggcaatttcc tcgccagtgc
    15961 ccaggttgaa cacctctccc aggctgtttt gattctccgc tgcttgtatg aacccggcca
    16021 cggtatcctc aataaaggtg aaatcacgcg tggcttccag gttgcccagt tgaacaacat
    16081 cgcggcaaag cgcctgcgtg atgatggtgg ggattaccgc ccgtccggat tgtccgggtc
    16141 cataggtgtt gaatgggcga acggtaacca gcggcaaatc atacgcgcaa gagaagcttt
    16201 cagccagttt atccgcgcct attttactgg cagaataggg cgactgaccc tgcagggggt
    16261 ggtcttcgcc aatgggcgcc accctcgctg tgccgtacac ctcgctggtg gaagtgtgca
    16321 ccagccgttc cacgcctgaa tcgcggcagg ccagtaaaac gttgagcgtc cccatgatat
    16381 tggtcgatac cacttcagcc gggtgtttat atgagtaggg gatggaaatc aacgcgccca
    16441 ggtgaaaaac cacctcgcag ccttttacag ctgcgcgcac cgcgtcctcg tcccgcaggt
    16501 ctccggcaac aagctcaatt gattcggttt tgcgggcatc taacatgcgc agcaggccgg
    16561 gatctccacg cgagttataa cggacaaagg tgcgcacctc tgcccctctt tctaccagcg
    16621 cgcccaccag gtggctgcca ataaacccgc ccgccccggt gaccaatacc ttctttcttt
    16681 gccagtgctc aggcattgtt ctccattacc tcctgggtct gcttgctcag gtaatcgttc
    16741 actactttta acaggtcagg gatcgccaca acgcgcacct gcagctcttt acaggttacc
    16801 agaatgcgtt cacgttctgc gtcgcttaac ctggtaatgg ccaaaaggac caaaccgaca
    16861 ctcttctgtt taacgatttc tgggatttcg cgtgtggtgc ctaaaaccgg gtacccgttg
    16921 atgttctttt ttagtttcct gaaatcatca tccaccaatc ccacaatcga gaacgctgaa
    16981 tacaggctgc tcttttccat caaccagatg gccagctgcg cggcatctcc cgcgcccacg
    17041 atgagcacac gctcgccaat caggctggat nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17161 nnnnnnnnnn ccaaggatga aagccaatcc aacagccagc agcaacatgc gtccaaatcc
    17221 cacatccagg gggatctgaa tgcgccataa gagcgtggca atagagattg ccaggaaagc
    17281 gatgatcgaa tctatcatga accaggagat gtaagcgcgg ccaaacctaa ccaacggtcc
    17341 ttcaaataag gtaccttcct ttactttacg ctgccgcagc cccgggatga gcattacgat
    17401 ggtcatcaag attacgtcta tatcgccaaa gaaattgaag tgccgcacat agagttggtc
    17461 caggcgcaat ttttccggca agatggatat gaggtaatca tccaacacgc tgctgcctac
    17521 cagcatattt tcctcgtcac ggaagatgac cgacgcgggg ctggtcatcc cggggcgcac
    17581 ggaaagtact tcggttttaa cttcttcggg ccagttatcc acaaattcag ggtcttcagg
    17641 gcgcggcccc accaggctca tttcccccac cagcacgttc caaaaattgg gtaattcatt
    17701 catctttgtc tcgcgcagcc aggcgccaaa cggcgttacg cgttcatcat ttttcaccgt
    17761 caaccgcaaa ccgctgtttt tttgaggggt ttcgtacatg gtgcggaact tgaggatttt
    17821 gaagggtttg ccatagcgcc caacccgctc tccgtggtaa tacaccggtc caggtgattc
    17881 acgttttata cgaatagcga tgtacaaaaa caatggcgag gtaatcagca ggccaaacgc
    17941 tgcgcccagc aaatcgacca ggcgtttaag aaaacgcaac aaaacgctcc gctgacgcgg
    18001 taaaacccag gctgaaaatc ccctggctgc ctgcttgtct aagaaatcaa cagttcggct
    18061 caattctggc tctgaactct atccagcgac gcgtggcata atcgatagaa aatgaaatct
    18121 gatcaacgaa tccatttaat agtatatcct aaaacttgat tgaacctcca gattcaaaat
    18181 gaccatatat ttacaaaact tcgttgattc acctcgatgc ttccatgcag gctaactctc
    18241 agggagtctt gggatccttt tttttccaga agaagaatgg cgtaccttta caaaatgcgc
    18301 cggcatttcc actggcagct ctcggcgctc aagcatttgc aacaagacgc gtacccgttc
    18361 gcttcccggc aacgtagcgt ccagaatggc catataaccg gcgaacggtc cgctttcgat
    18421 ttcgacctga tcgcccgcat gcaggtccgc cgcggactgc cccccctttt cattcatcgc
    18481 gtccactttt cgacggatgg catgaagtat attatccggt acatgtgaaa actcgccgcc
    18541 aaatgaaacc aggctggctg ctccgggaac gtgttccaga gaaagaacgt tgacctgctc
    18601 aaggtcaagg tgaataaaaa ggtagccagg gaagtaaggc ttgaagcggc gcgcgcgtgg
    18661 gttcaccggg ttgacccgca gtcgtgggta gtaatactcc acattccgag attcaagctc
    18721 ccgcgctaga aactcttcgc ggttgggttt gctgcgcatc acataccagg aaattgtcat
    18781 cgatattcct catccccttc aatcgcctgc ggtttcccct ttaacacgcc aatttcctcg
    18841 taacccctgt atttcactta ttctttccca cccctgtcag gccatagcca ttcagtcgcg
    18901 atgccgccgt ctcattcaac caaccccgca tgcccgctgc aaaatcaact cttcccaacc
    18961 catagcccca agcacggctt tccagccagc tgaaaggcgg aaacctcttg aaagaacaga
    19021 aatattgaaa ccagcgaatg cctatttatc acgtaacgcc cggtctaaca gtgagaacat
    19081 tttctggtta ttgttggact cttccagcag gcggaagttt agaacatgca agataatccc
    19141 taccgccaat aaaattccag caaatatcat tagcccggta gctaacaccg ctttgggtac
    19201 actgtaaata tattgatacc ggatatactc acgaatcaca aaagagccca caaataaact
    19261 ggtgaccgcc agccccaggc tgatcccgcc aaaaaacgtg agcggtttat aagagggaat
    19321 aatcgaaaag attttattga tcactttaag accatctgaa aatgttcgca acttcgagta
    19381 acttcccgtt ggtctttccc ggtagttgat ttcaacttcg gtgatcttca gatggcgata
    19441 aagtaattgc agggtcattt ctgtttcaac gtcaaagcct gtggcgacta ccgggcattg
    19501 catcgtcacc ttcctgttaa acgcgcgata cccggacatg ggatctttga ggttggtcga
    19561 aaaaacagaa ttcaccaaag aacaaaccaa cttgttaccc aggacatgga gaggaggaaa
    19621 agccccctcc gtataactgg acagcctttg cccgacgacc atgtccgctt cttcgtttac
    19681 aaccggggca atcagctcgt gtacgctgct ggcggggtaa gtatcatcgc cgtctaccat
    19741 cacatagata tccgaaacga catcagtgaa catcgagttg atcacatacc cttttccctg
    19801 gcgtttttct tttaccacaa tcgcgccgtt ttcagccgcc aggctcgctg ttgcgtcctg
    19861 gctgttgttg tcaaacacat aaatatccgc atgcggcaat tctctacgaa aatcattgat
    19921 caccttggca atcgtttgct cttcgttaaa acaggggatt agaacagata tttttggttg
    19981 tgtccgattg ggagttttga ccatttttca ccctgaaccg caagttttta tcgtaatgaa
    20041 ccaggaacga cgctgtgttt ttttaattat atccttgact ttcactacgg tatggctgtt
    20101 aacctacaac cattcttcag gatatcgctc aaatgctgaa aacaaagatt ccttttacta
    20161 ccttacaggt atccttcacg gaatatctcc ttgaacgaag ctgaattgtg agcacgagct
    20221 cgtgtatcca gttgcgtgtg aactgaattc caggaaggaa acaactgcct gaagagtcac
    20281 tcttccttga tggcgaccgt aatgagagac aacccgatcg gcgggtggat tattttttca
    20341 agtctggaaa ggatgggcac cagagaatca aaaagttgaa tttgcccttc attttgcctg
    20401 acagaacgcc ttaccttggc attaaaaagc cagccccaaa aacccaaaaa gttcgtgtaa
    20461 aaaatggttt ggagttttag gttcgtctcc gctaaggctt ttttcatggt gcgcgcagtg
    20521 tatctgcgga agtgtccaac cgcctgatcg attggtccaa atgccacagg cattgccggc
    20581 accagtagaa ccacctttcc ccctgtagaa aggatttctt caaaggtttt aagaacagag
    20641 acatcgtttt ctatgtgttc cagcacattc atacacaata tcgtgtcaaa acgataactt
    20701 ttaagagccg cagtgtcacc atcttctatc ttcgtgtgca ggatagtgta attcgatgat
    20761 ttcggcatca aatcttttaa tacatcgacg cagtatggat tgggttccat gcccaccagc
    20821 aattccacat gattgagcat taaaccagtc atattaccaa tccctgggcc aatctctaaa
    20881 actcgctgtc caataaatgg ttctacaagt gaatacagcc agcgattata gttttgggca
    20941 aatttcattt gttctaaatc atctctgata aacgcatctt ctgtttgagt aattctgcgg
    21001 tctttttttt gtgtcatgtg ttaatcaatt cttcctgcat gatcgattgt ttatggtcaa
    21061 agaatctgcg cccgcttcac tcatccagct gagtgcgaga actaaacgga aaagttacgt
    21121 tcctgaggta ttaaacagaa accatccgtt tgggcgatat gaatcaaccc cattacctgg
    21181 cgctaacaac atatcctttt acgctcaacc agagcgatta tacccgtaat gaaaatctct
    21241 ttctcatcag cttttttccg tcgaccattg aagcataaaa attcggcttc ttagaatttc
    21301 ttgtttctac ccttatcgtt aacctttact cgtcacaggc ggaaaattac aacctcatcg
    21361 gttgaagtta gaatccgtct tttttgttcc ctcaacagct ctatggatgt gactgaactc
    21421 tcataaagac aaatttcagt atgacaattc tacccatctc gcgtttttga tgatatattg
    21481 gtctgggagc ggctttgaat aatctcaacc attccaactg tacgataatc tcctgatatt
    21541 gaggttcgat gaaaaaggtc gagaatatcg cttacgctgg tctaaccagg tttatcgaaa
    21601 acggagaagg agaaaaagag ctcctcctgt gggttgaaaa tgacctctcc aaaacgtctt
    21661 tttcttacag ctatttcgaa accatgtcta accgcatcgc cgggattctt caggaaattg
    21721 gcgtcaaccc cggtgacact gtcagcttgt ttatcccgcg cagcccgatg ctgatcagtt
    21781 gcttttttgc tgtgctaaaa aatgcggcta tttcaagtat cctctttagc acgcttggag
    21841 aagcggggtt actggacagg ctttcaaaca gtaacgcccg ggtcatcatc acaaaaaaga
    21901 gccttctcag gaagatcatc tcaattaaac accagctccc aacacttgaa cgaatactgg
    21961 tggtcgatat cgacgaagat caaaacgacc atgtgctcag cttgaaacgt ttgctttcga
    22021 aaagttcctc agctttcgat tacccccatt ccctggatgc agaaaccccg gcgttcttac
    22081 aatacacctc cggatcaacc ggaaaaccaa aaggggcgct gcatgtgcac ggggcgattg
    22141 ctgatatcga aaaatcgttc aaagaaatca tgcagctcca ggcggatgac ctgtactggt
    22201 gtacagcaga tccagcatgg atcaccgggc tggtatatgg catcattgcg ccattcacta
    22261 ccgcggcgcg tcagcttcaa tataatggca cttataacgc cgataactgg ctgaagctcc
    22321 tggagcaaga gaaggtgacc gtttggtata ccgcccctac agcgctgcgt atgttaatgc
    22381 aagaggaaga gagtaaattc tctcagcgag attttcaaca cctcaaacgc atgtatagcg
    22441 ttggtgaacc actaaatcct gaaatctacc attggggcaa gcgaattttc ggcgtggagg
    22501 tttatgataa ctggtggcaa tcggagaccg ggtcaatgat gattgccaac cgaccaccaa
    22561 tgaaggtcaa gcccgggtca atggggaagc caagagaagg cattgaatcg ttcatttgcg
    22621 atgagaatat gcggttgctg ccgcctggca agcagggcct gttatgcctc cataaaggat
    22681 gggggtcgat gtttcgcggt tatttccaca atgacacggc ttatgctgaa aaattccacg
    22741 gcgatattta cgttgccgga gacctggcgt ttacagatag cgatgggtat ttctggtatg
    22801 tgagccgcgc ggatgatgtg attaacacag ccgggcacct ggttggccca tttgaagttg
    22861 aaagcgcgct gttggaaatt ccagaaatcg cggatgtggc tgtaattggg gcggatgacc
    22921 ccattatgca taaacggatt gttgcgtttg tgaaattgcg cagcggcacg gcctggagca
    22981 aagagctgga actcaaatgc cgcgttcatg tgtcaaataa agtatccact tccgccatcc
    23041 ccgctgaatt ccacatgatt gaacaagtgc ccaaaaacca aagcgggaaa attctacgcc
    23101 gggtgttgaa ggctgtttat gaagggaaag accctggcga tacctcaacc atggagtgaa
    23161 agatgaatat tcaggaaaga ttaacgaagg ttttccgcgc cgtgttcgac aatgaatcaa
    23221 tcacactgac ccctgattta accgccgatg atattgacga atgggattct ctctcacaca
    23281 tcaacctgat gctcgctatc gagctcgaat ttggcattga atttttacag agcgagatcc
    23341 aagattttcg caatgttggg gaactgattg agtgtgtaaa aacgaaactc gagccatctt
    23401 aaaccttaac aagccgcagc gagagagaag atcaccttca ctggggtttt gcccccagtt
    23461 gaaaatggac gataattcgc tatttttaag caatgtcctt tcggtgccaa ggtaggaaac
    23521 acatgctgaa gtatctaaaa atctcctttt ctttcattcg ccgctatggt ggcatttttt
    23581 tgttgttcat tccaggggtt gtgatcctta tccatatttt tggtatcacc ttatccagaa
    23641 taccaggcga tctgggtgat tcccgtatgg tctggtatat gcttgagcat ttctacgcct
    23701 ggatcagcgg aaaaattgat tcctattgga acgcgggttt cttttatcct tacccatcga
    23761 ctgccgcgtt cagcgagacg atgttaggtt cagctccgct gtacgctctt tttcgattcc
    23821 ttgggttcag caaatacctt tcctaccagt tctggttgat actggggttt atgctgaact
    23881 acttcaccgc agcttttatc ctggtgaagt gctctcttaa accactggct gccggcgcag
    23941 gcgcgttttt cttcgccttt ggaatgccca tttttggtca ggaataccac ctgcaactga
    24001 tactgcgttt tcttgtcccg gtggcgggtt acctgctgtg gcgtttttca cagaaacctc
    24061 gctttgtgtt gctgcccctg ttgttcttcg cggttgtctg gcagttttat atctcggtct
    24121 atactgggtt ttttcttgcc atcatgctgc tcgtgatgtt attcctcatc ccttttatta
    24181 atggaggaga tacatcccgg aatccatttc ggttttggag ttccgcgctt gtattgtctt
    24241 tcaagagcac cagcatcccg aagcgtattc ttcacctttt cttaatggcc gccagtctgt
    24301 tccttttggc gaagttattc atgccttatt acgtagttac tcaggaatat ggatttaaaa
    24361 ggtcgataga ttacatcatc accatgatgc ccaggctcga aagtttcttc ctcgctgatg
    24421 gctcgcagat ttggaacacc aaaacctgga tcaccaagtt catttctcat cgtcatgaac
    24481 agcaattgtt tccaggcctt tccgtttacc tcttgatcgc ggcagggctt gtttggaatt
    24541 tccggcatgc caataaacgg ttcgctttag tcaatctttt cacagtggtg gtcatcctct
    24601 tgttgacaac ttacaacaat ggattttcac catatgtgta tttactgaat ttacccgggt
    24661 tcaatagtat ccgcactgtt tgtcgtatcc acctcgtttc aatgtggcca gtggctgttt
    24721 tcgttgccac tgtgctcgac gcattattga gcgcgccggt aaaaaaaatc aattttcgat
    24781 gggtttcatt tttgctgatc gggtttatgt tgatggaatc tctctatttt gatcacccca
    24841 cttatgaaaa aacccaggct gctgagcggt taagcgtcct gtacaatcag attgaaaaga
    24901 aagggttgat agttaaagaa gattccgtcc tgtatgtcgg tgaagttcca ggagatatac
    24961 cctacgccac cgatgttgac ggcatgtggc ttggcctgga attaggaatc ccaacgctca
    25021 acggcatgac cagtaatttt ccggatggtg ccgttgtcgc ctcttctgat tgcggccgga
    25081 tcgattcctt catccggagt tatatgaaat ttgccagtat taaggaagct cagtttcgcg
    25141 accagttatt caagcggctg attatcttga acatggacca ctgtaactat tgagctgcgg
    25201 gaacggacga tcgagtttta gatcagttgc gtaattcaaa gaaccacgca ggtgtcgtcc
    25261 tgcaaactca ttatttcctt aattgggtta gaacttccta tcggttagat tgataaacag
    25321 caaaagccag cgtgaaaaca ctacgcccgc tcctctttac gagtgatagg atttcctttc
    25381 tttggcagaa ggcaataggg aatgtttgtg taggataagt gactggcaaa aaagggatcg
    25441 ccgccctcaa aattatcgat gatttcctcg catcctaact gaatatcatc ccggggcgtt
    25501 tcaactcctc gggaaccgcc ttcaaagtgg atcagtgtgg cgtaaggatt gtacaggttg
    25561 cgaaagccgg cattttctgc ccgtaaacag aaatgcacat ctccaaacac caggcggtaa
    25621 cgctcatcat agcctccgag ttgctcaaac aaagttcggc acatcgcctg gcaagcacct
    25681 gttaccgcgt gaaaattgcg gtaccacatg accgaaccgg tcaacccaaa gtagttctca
    25741 ggtgcgttca aatacaaatg accagaaaaa ttgttcatgc cgataataaa acctgcgtgc
    25801 tgtatcgtgc ggttgggata gagaagcctg gcaccaacca ccccaatttc aggatgcgaa
    25861 gcccattgca caagttcgtt caaccatgaa ggttcaatga cctgcatgtc attattgaga
    25921 agaacaacaa tttcagcgct ggtgctcgat acgccgagat taatggcagt tgaataatta
    25981 aactcctggg gataaggcac caccttaacc tgcgggttat cctccagctc ctgatagtaa
    26041 ttaagtagct cggtctcagt gctctgatta tccacaatca cgatcgaata attcgcataa
    26101 tctgtacgag tttggatcga ttggacgagc gttctaagcc aattaccgtg attcctgctt
    26161 aatataatga tcgcagccga cgggttgcca tgctgccagt taatttttct gccattcccg
    26221 tcatgttgaa tttccacatc tctgaagcct agccccgtgt agtgattttt gagtacaggc
    26281 tgtagcgcat ctatgctggc atctctctct tccggaatag ataccaggac ggctggaatg
    26341 tgctggatgg aacaatgacg cgctgccaat ttgagcgcga gcgcgtattc cagcgcgcgc
    26401 aagttgctga attcttctat ctttaccttc acagcagcca tacttgcatt gcgaaacacg
    26461 ccgcgagaaa aatagttatt ggagattaat aattcagggg tcagcgcgga gggcttaaaa
    26521 aaaggttttg gtccgcccac agaactcaaa tattcacagt cataataaat gatatctttt
    26581 tttaccccac tgcccacgat ggcgaaataa tgatttaaaa gtgatttgtc gaaaatatcg
    26641 cctgaatcac aaaaaacgct cgcgccatcc tggtcagtta tgaactggcc tagcgcgaac
    26701 tcgttccatt gaaccgctgg ttttatcctg gggtctgctg acctctccgc gctttgccca
    26761 atgggtattg gagaatcctc caacacggga acaacctgcc agtaaggcga tgccaggttt
    26821 tgcaaggaat cgatcgttct gtcagcgagg ttttcagttc cttttcttac agccagaaaa
    26881 aaagtgactg ctggcaaaac cggtgagcca gtgaaaatcg gcaggggggc gttgtcctgc
    26941 agcttcttga accacctata ggatatgttt gccttgcgga ttttctgctt gacgatgaat
    27001 cgattcgcca taactttatg gtaaagggtc cgcaagaaca attcgtggcg gcttccccga
    27061 gggaagaata tgtcgaagat ttttttgctt gttctatctc gcagcatatt cgtattcaag
    27121 taaatccccc aaagcgcaag aaaggatgcc tttgggtcgt atctctatcc ttggcgggaa
    27181 ttgaaaatat ctttgttctg gcacagttat ttactcgttt tttctggttt ttagaaataa
    27241 ccctttcgga ataaaacaag gctcagttcg ttgtctgtgt caagcgaaca aatgcctgca
    27301 gatactgatc tgcgatcttc gtccagttat aacattcatc gaccactaag ctgattttct
    27361 cccggtcaag gtgaatgttc aaaatggcat ctgcaaaact cgttgcgtca ccctccggca
    27421 ccaaaatccc ggttgcgcct gggatgaccg cgcttggaat gccatcgaca tcagtcgcta
    27481 cgaccggtaa attggtataa ccggcttcga ggatcacaat gccgaaacct tccagatcgt
    27541 tcttgatgga tatgttgggc ataataaaca agtcagcgca gcgaaacatg gccaattttt
    27601 catcctctgt gatccagcca acaagataca cacggtctga ccactctttt tgttcgatga
    27661 tttccaaaat cctctggcgc tgcgggccgt taccggcaat aatataaatg taatcttccg
    27721 gtaaaagagg gaaaacctct tgcaggaacc aggcattccc tttcctttcc acaagcctcc
    27781 ctacactcac caggattttt ttgtgaaggc tgttaatgga atgttttgtt aaaaactcct
    27841 gagcgctttg tgattctatg attggcaggt ctttgagatt gatgccattt gggatgtgct
    27901 gtaaccgttg cggattgatc cctctttttt cacattcacg tttcgtatta aggcttacgg
    27961 ggaataacaa atcagctctt tttaagaaaa aaggaatgat caattggtag agggggtgat
    28021 gataaacgat atccaggccg ttaatgatga aagtgatttt tgccctggaa aagaggcgaa
    28081 taaccgggat aaaaggcgcc atcaccgcat cattgaaatg gattatgtcg aaacggcttg
    28141 aatgaaagat caaataaaaa aatgtttcga tgaaaaactg gacaagccgg ctttttccct
    28201 gcgggttcgc cagaagatgt gtttcagtga tcgccgacat gtgttgatag aagttatagg
    28261 cgttgttctc cagcccccca atgattggcg ggtaattcct ggcgatgaac aggaccttcg
    28321 gaagtgttct ttcttgggtc attgcggttt atccttcgga gttgtgttct ggtagcgcag
    28381 gctgcggatt tcataaagga ttttatcctg gttggtcttc atacggttca acatgtctgc
    28441 caccagggca atcaccaaga taatcaaccc gaaaagtccg aaccccaaag cgataaaccc
    28501 aaaagattta tatggagtga aactacccgt gaggatataa tgaaaaaaga gaaacagacc
    28561 gaaacccatg ccgatcgcca ggaaaccacc gcctatcacg ccaaagactc tcagcggtcg
    28621 atagtccagc agcacgcgaa gaatgatctg gctggttcgc gcagcgtact tagaaattga
    28681 tccggcgacc cttgattttc tatcggtgta ataggttact gcgattggaa tctcaaccac
    28741 ctttagcccc tggtaaaccg atgacagaat ggtttcgtgc gtgtatgtga actctccata
    28801 gatgttgaag cgcagcaatg cttcgcggcc ataagcgcga aaaccgcagg acacatcggt
    28861 aaatacttga cctgagattt tgctgatgaa cctggagacc atcttattcc caatgaattt
    28921 aattccaggc atgttcgcag gcttgccaga caaaaaacgg tttccaatca ccatgtcggc
    28981 tttgcctttg aggaggggag ctatcagggt gggaatttcg agcgggttaa actgaccatc
    29041 agcgtcaatg cccaccagga tgtcacagtt atgctgcagc gcatattgga cagcggtatg
    29101 aaacgcagcg ccaactccgc ggttgcgcgt gtgagataca acttccgcgc ctgccacgct
    29161 ggcaagttcg gcagttttat ccgttgaccc atcatccact accataacag acacatctgt
    29221 aaccccgtca tacattcttg gaatgttggc aaccgtttct gtgatacgtc cttcctcgtt
    29281 aaaggccggg ataaaaatga tcattctcat tagaaactcc gatacgggct cttatatgca
    29341 aaacataacg tgcagcttat caagattata tcctttcgtt ttccttttgt gtcacaggaa
    29401 tgaccatcgc attttacgac gggcatacaa aaccggttgt actgcttttt tattcaccga
    29461 tgatcataac cagaaaatac aactgctcag gcaagatgac cttcctcagg cttgccgtgt
    29521 aaaagtgatt taagggtcag agcctgggga atcagcctct tatattgcca gcggttcttc
    29581 ttgaaaaacc atggaacggc tagttttttt taattttttt gaggagattt tcaatgtcgc
    29641 gaagcggctt tatccatttc cagattttcc gcttgatgaa gtgaaccagc tgatactctt
    29701 gctggtgcac ctcttcgtgg aacacatcgc gcgaacctga cagttggcta aaagtgttcg
    29761 gggtaaattg atagtattgc ttcaaagccg ctgcttcccg cggatcgttc agcaggtcga
    29821 acaacacctg taacccgaac agccagcata ccggatcatc ttccttatcc agcgcggcgc
    29881 gcgcgatcaa atagcgatga ttatagtact cccccacttc atacctgata atggcgggta
    29941 acacccgcac cagttcatcg cgattcgtta gagccaggta attctggtat atttgcagct
    30001 tttcaaagtt gctgcgaatc aggttgatca gcgtggggct gctggcgtta gcggagtcaa
    30061 gcagcaagcg gtaattcacc aatttttctt gcagcacgtg gatctcgtac ttcaggcaca
    30121 atcgcaccca catatcaaaa tcaggcaatt gcaccaaacc gcggcggtag cagccgcatt
    30181 tttcataaca ttccttgcgg atgagaacgc tgggatggca gagcgcgtta ccatagtaaa
    30241 aaaagtagtt caaccagcca aaacggtcac ggttgggctg gttgaagaag ttaatgggga
    30301 aagaaatatt cccgctgatt gggcggctgt tttcatcgat aaacaccacg cgcgtgaaca
    30361 ctgcgccaac ttcggggtgg gcctccaggt aggcgacctg tttttcaagc ttggtgggtt
    30421 cccatacgtc atctgagttg tgcatagcaa cgtattgtcc cctcaaatca ggaaggaaat
    30481 gaccaaattg gcgctgacgg ttcacctcgt ttctgaatgc cgttatccga gagtcagaat
    30541 aactgcagat gatatcccac gaagaatctg tggaagcgtc gtccttaata atcaattcat
    30601 aatcagtgaa ggtttgcccg agggtactgt cgatagattc gcgcagaaat ttttcgtgat
    30661 tgtatgagtt gagaaagatg gaaatggttg gagttcgccg catatcttct gtttccttaa
    30721 cccggggatt cagccattgg atttttgtaa aaactcctcc ttcaggatag aaaagagcaa
    30781 gacatcaaag aactgcccat cctgaaaaac agcctttctt aaagtgcctt catatgtata
    30841 tccagatttt attcctgatt tgatcgaacc ggcgttattc gctatgatcc tggatctaac
    30901 ccgctccatg cccaactcaa gaaaagcgta tcgaagaatc atgatcctgg cttcggtgcc
    30961 gaagccacct ccctggtgct ctttgcttcc gattaaggta tgaatctcac atgaccggtt
    31021 ttgataatca atatcagata aatggatgta accaatgagt tgattgtttt cttttaagca
    31081 tatggccagc cttaccgatt tcccggtctc atgctcctca attatcttaa gcacccattt
    31141 ccgttccgtt tctttgctca catagcggtg agtttcatat tgaagtgctt caaagttact
    31201 tcgccaccta taagaagtaa gatagtcctc aggctcaacc gcccgcagat acacacggtt
    31261 tggactggta ttctcaataa tgattttttc ttttgccatt tattacctgc ccaccctcaa
    31321 cttgtcaaga aacaatttac cctgttctat tttcgctcac tttgcccata tctttccatg
    31381 aattcttcct tcaacattga gtaaatattc acatcgataa gctgcccgtc ctgaagttcc
    31441 gcctttctaa gcaccccttc cttcacttaa ccaaatcgct cgccattggt tatagccggg
    31501 atattcgtac ttaaaatttt cgcccagacg cgctccatac ccaggttgag aaacgcgtgc
    31561 tgaaatagga tgatcctcac ttgcgccggg attccctttc cgtgataatt cctgtcacca
    31621 ataataatgc tgaactcgca gttccggttc ttgtaatcaa tgttcaacag gttggcataa
    31681 ccgatgagcc tgtcttctcc aaccgtgcag attcccacgc gcactgaccg cccggattca
    31741 ttctcttcga tcactttttg aacccatttc ctctccattt ccaggcttac atatctcgga
    31801 gcggtatatc ccttgatcag gtcaaaatca ttgcgccatt tctgtgaaac cagatagtca
    31861 tcaggttcaa tcgcacgcag gtacaccctg tctttcctgt tcatctccgt aacatttttc
    31921 atcgtttttc cccattcgcc cctgtcactt agagatattt atggattgcg ttagttctat
    31981 ttactctaag tctctggcgt atttttcgat aaactcttcc ttcagtatcg agtatatatt
    32041 cacatcgatg agttgcccgt cctgaagttc cgcttttcta agcacccctt ccttcacata
    32101 accaaatcgc tcgccttcaa tgatggcagt gatattggtg ctcaatacaa aagcgctgat
    32161 acgctccata cccaggttga agaacgcgtg ccgaaataga atgatcctca cttgcgccgg
    32221 gattcccttt ccgtgataat ccctgtcacc aataataatg ctgaactcgc agttccggtt
    32281 cttgtaatca atattcaaca aattggcata accgatgagc ctgtcttctc caaccgtgca
    32341 gattcccacg cgcactgacc gcccggattc attctcttcg atcacttttt gaacccattt
    32401 cctctccgtt tccaggctca catatctcgg agcggtatac cctttgatca ggtcaaaatc
    32461 attgcgccat ttctgtgaaa ccaggtagtc atcaggttca atcgcacgca ggtatatgtc
    32521 catgcttgtt atgttaaaac tccttaattt ggaataaggt tattacacat caatatacat
    32581 taattcagca actttttcgg ttaagaagca gccagcccga gtaaactatc tcctgtgact
    32641 tttcaggatt atcttcaata tccgttcaat gtccgtctgg ctgagcctct ggtcaaccgg
    32701 taatgcgatc agcatcctcg ccagttggtg ctcccagctg ccaggctcag ccagctcgcg
    32761 cacccctggc cagtagcggg ctgtgtaaat tttctcatcc agcagtctgt catacaggtg
    32821 aacatcatca gttagaaacg ggtagattag gggcacttgt gtaccattcc agggcacctt
    32881 gagcaggttg tggttcttta acgcccgttc ataataacca aagttgtcaa ctctcacacg
    32941 cctgatatgt tcatagtcaa tattcttaag cagagccctg gtcaaacccg acatggttct
    33001 taaaggctga gaggataaat gctgttcatt ctggcggtaa aaagcaaaac catcttccgc
    33061 agaaacgtca attctcctca acaggtgttc cgcccgatga taagaatggt caatttctaa
    33121 cgccaagttc actttggttt tcaggtaaag atacgcgcca tcgggcacgc cgaagaattt
    33181 ccgagttgaa tagaaggtat ctgtatcctg aatgggtttt gaaaaaaagg attgcgcgtt
    33241 gtccacaatc acattgcctg tctgttttcg cagcgctgcg attgagtcgt cttttaaacc
    33301 aaagtaattg acaatgagga atccctcacc ttccttgagc gatcctatct caaacacagg
    33361 gtcaagatga tggtcaacag gatagaactg gtaagggatt tgcagttcct taagtggctg
    33421 caacacagag tcacagttat aaatgggcag gtaaatcttc tgatattttc ctgtcagcag
    33481 gatcgtcttg aaagcgtttg cgccggtatt caccctgata gcgttcgtgt ggtattcctc
    33541 gccatcacgc aactccaacc ccaggtaccc gcccaaaggt tttaacaaat gacgttgagg
    33601 agcgcgatca actttcacca tggctctatt ctttcaacgt gaactcaccc tgcgatcgaa
    33661 cttattaacc acctggattg aattcgccca ggcggtaaag cggaaagtaa ttactcccgg
    33721 cggcgttctt ctgatcgcag agttcctggt aaacgccttc attcagcacc ctgccgcaaa
    33781 aataaaccgg cttgattaag gttgtccatc gttttttgta ttgtaccagt ccattttccg
    33841 cattctccag accagcgccg gaaccctggt caaaaaaacg caaggtatct gcataatatt
    33901 ttaagatgct ccaatcaagc gcgtatgacg cattatgttc gtatccagtt gagctgatgg
    33961 ccagcaggtg accatagcct atagagccct ggcgcataac gatctctgcg ccaacgatct
    34021 cacctgccag acgagcgaga aacaggtcaa ggccaggtag gctgagcagt tgagaaaaac
    34081 tgttcctgga aaatgcgcgg atgcccgtga tattgtgcct attaatcaat acagtataaa
    34141 gatcgaccca ctcatcaagg aactgcgcag gttcgtggca tctctcaaca acgatttccc
    34201 gtagtgccct ttctgcaagt tttctggttt ttctgctgat tgtttgttca agcggcagag
    34261 ataaatccgt tatgtaatgg tctttgtaat ggtaacaaag aggaaaggtt tcactcaatt
    34321 gttcaaaaga gccttgcata aaagggtctg ttaccagtgt tacgctcacc agctcatgat
    34381 tcaggtcgtg aatgtcctcc cttaataagt tccagttttc acacaggaac aatgggtaac
    34441 accccatcgc atcgaaataa ccgctccctg ggacgggtct tttgatcagc catcccttcg
    34501 agcggggtaa gaagagcgct gtcccgactt cagagaaaga atccgcgtag ctgggatgca
    34561 ggtagccttc tgccatttgt ccttgttctc cgctcctcaa tcaaagcgat gcaagtatcg
    34621 tttctgtgat caacgcaact tcattttcgc tcaaacccgt atacaaaggc aggcacaaga
    34681 cccggggaga aatctcatcc gatctcgggc agcgttttgt cgaaacataa ggcagagaag
    34741 tcagtgaagg gtaaaaatac ctgcgcggga agatttgccg gtcgttcaac gcttctctaa
    34801 cccgcagcag gcttttttct gcagggaata ataccgggta atacgcgtaa ttgtattcgc
    34861 tgccttcggg aatcaaaggc cgggtcagcg ctgagccagc caaacgctca tcgtaccacc
    34921 cgcatacctg cttgcgcgcc gcgatacact ccgctaaatg gggcaggatg caaagcccca
    34981 tggcggcgtg aaactcagaa tttttaccat taatacccaa cccccagaag tcttcctgcc
    35041 ccttatgacc gaaattgcgc atataactga tttgatgcgc gacctggtca ttattggtga
    35101 tgatcgcccc gccttctgct gtgtgaaata atttcgtcgc atgaaaactg agcgttgaaa
    35161 catcgccata ggaagccaga gaataacctt tatagcgcgc accgaaggtg tgagcagcat
    35221 cgtagatcag cttcaggttg tgcttcattg cgatgcgctg aagttcagcc acatcacaag
    35281 ggatgccgta tacatgagtg gccaggatgg cggttgtttg aggggtaatg gactcctcta
    35341 ccaattgcgg gtcgatgcac agcgtgcggg ggtgaatatc cgcgaatact ggcgtgcacc
    35401 cttcccacac gatactggag gtggtggcca cgtatgtgaa aggcgtgctg atgatctctc
    35461 cgcgcaagcc cagcgccctg atcgcaattt gcagcgcaat cgtgccatta ctgacaaaaa
    35521 acaagtgccg aacccccaga tattctttca gttttcgttc cagttccaac accaatgggc
    35581 catgattggt taattgatgc gattcccaaa tctggttcaa gtaatggaga tattcttcca
    35641 tggggggtaa ataggattga gtgaccggaa tcatgtgacc tgctttgatg aaaaagaaac
    35701 aaactcaacc atcgcaaatg gtgctgcaca acttatattt cattatacct tgcaatggtt
    35761 tgttagattg aaacgctgtc cagacaattc atcaacagga gaaactcagc cagccgcagc
    35821 ctatcaccca atcatgaaaa gcctaaactg aagtattgta taatcctaag caatatgttc
    35881 tttttttccg tgttctttta tgttgctatc gctcttttac tcgtcctcag gcattccaac
    35941 gagctaagag cgagccgctt cattatttct cttttcatca ccatttctgc gctaaatatt
    36001 ctggtcatcc agaccctgca ttttctgcga ttgttggata accccctgac ttacctgttc
    36061 gtacaggttt ttctttgcgc tctttgcgcc ttgttgctca tagatccgaa aaaaaagctc
    36121 ttcaaaacgc ccttgaactg gccccaggtt tcttgcagca aaatctcttg ggtcgagggt
    36181 attttatttt tctttttatg tatcgttctg gctggtgtat tttacgtagg aacactcagc
    36241 ccgatcaata actctgactc gttgcaaacc cacctgcccc gtatgtttta ctggctgcag
    36301 caccgctcgc ttatcagctg gtaccccaca gccgccaccc aactttcata tccagtcaat
    36361 atcacctttt taggtttgtg ggctttcttg ttgagcggtt ctgaaatgta cctgttcgtg
    36421 ccgatgtggt tgtctctcgt tgcaatcgct gctctggtct tcgaaatcgc cctccttctt
    36481 ggcgcacaac gtgaggcggc catcacggcc agcctagttg ctatcagttt gcccgtggtg
    36541 ctgatgcaaa cctactccta ccagggagat gcctttgtgg ctgcccaggt gctgtgtgtc
    36601 atcttttttc tactgcttta ttccacccaa aaaaagaaca ccgacctggt tctttcagcc
    36661 cttgtcatgg ccatcgccct gggctccaaa cagacagcca tccttttcct tcccatttac
    36721 ctgctctcat tgttgttact gattttggga aaaaccctga attttcgctc attcttgaac
    36781 actatcttac tcgccatcct cttctttggc gttttctcat cattgaaatt cgtgcagaac
    36841 ctgactgaaa cgcaaacaga cgcggcgcac atggtgaatc ctgg
//
LOCUS       DAOTLW010000016        35915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1413_length_35915_cov_10.414780, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000016 DAOTLW010000000
VERSION     DAOTLW010000016.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35915
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1413_length_35915_cov_10.414780"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            78..2150
                     /gene="fusA"
                     /locus_tag="P5516_04025"
     CDS             78..2150
                     /gene="fusA"
                     /locus_tag="P5516_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRT91638.1"
                     /translation="MKEYSTEKIRNIVLASHSGAGKTILCEAFLHATGATTRMGRIED
                     GTTVSDFDEEEIRRTISLSTSVIPVEYRDVKINVLDTPGYTDFIGEVISALRVADGAV
                     LLIDAVNGLEVGTEIAWDYCTRFNLPRFLLVNKMDRENANFRKALQTVEDYSETRLIP
                     IQLPWGEKQDFKGVIDLLSMKAYKAQGKTAEEIPADMKDAAEEARMKLVEAAAEGDDV
                     LLEKYLESGSLTEEEVIRGLTLVVQSGDFVPVLVSSGSNEIGVIRLLDAMVDIMPSPL
                     SAAKIVAQGKAGEEILEPKDNAPLAIYVWKTTADPFVGKQTFLRVYAGTISPDARVWN
                     QNKGIEERLGSVNVPRGKESLPVKVIHAGDICVVPKLTETSTTHTLCDKNHPLTLPVP
                     EYPTSLYQVALSPKTQADSTKISSSLTRLCEEDMTLSWHFQTATKQTILQGMGDQHID
                     VAIRRAESKFQVGILMEEPKVPYQETITKSASAMYRHKKQTGGSGQFGEVHLKVSPLT
                     DKEFDFTWDVFGGAISQSYSSSIQKGIQGVMKEGVIAGYPVSGVLVSVYDGKEHPVDS
                     KPVAFEVAGREAFKLAIKDAAPVLLEPIMLVKITVPEAAMGDVMGDLNTRRARIQGME
                     TQKGRSVVTANVPLTEMLRYTTTLRSMTGGRGVFSMEFDHTEVVPTHLAQAVIDAHTK
                     EMESRKEE"
     gene            2350..2679
                     /locus_tag="P5516_04030"
     CDS             2350..2679
                     /locus_tag="P5516_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HRT91639.1"
                     /translation="MDISQLTTEMNRFVSAKGWYAENSPRPQTARNLAISLVLEAAEV
                     LELYQWGESAVSNQELSDELADVALYLLQIASITGIDLEEAIMDKLKKNFQRSWDGAG
                     TERKEIK"
     gene            2676..3236
                     /locus_tag="P5516_04035"
     CDS             2676..3236
                     /locus_tag="P5516_04035"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015597.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HRT91640.1"
                     /translation="MNIAVFGGASVRPDDVVYTETLRLGKMLGGHGFTVLTGSYIGVM
                     EAASRGANEAGGHVVGMSCAEIENWRQVKPNRWVLEEHHYETLRERLFALIDGCDAAI
                     VMPGGVGTLSELVVMWNEMIINSKPPRPLIVVGSAWQRVMAQFFASLGDYIGVKDREL
                     IQLVPDVDTAVEILVDYQNQSIRESL"
     gene            3233..4666
                     /gene="proS"
                     /locus_tag="P5516_04040"
     CDS             3233..4666
                     /gene="proS"
                     /locus_tag="P5516_04040"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRT91641.1"
                     /translation="MSNEKLPSRAENYSEWYNQVIQRAELADYAPVRGCMVVRPYGWS
                     LWENIQQALDSRFKATGHVNAAFPLFIPMSFLEKEKEHVEGFSPELAVVTIGGGEKLE
                     EPLVVRPTSETIIGYMYSKWIKSYRDLPLLINQWGNVVRWEMRTRLFLRTLEFYWQEG
                     HTAHATAKEAEEETLRMLDIYTRFAVEDAAVPVIPGRKSNSEKFAGAVHSYSIEAMMG
                     DTKALQAGTSHLLGQNFAKAFNIQYLDMNNELQYCWTTSWGVSTRFIGAIIMTHGDDQ
                     GLILPPRLAPIQVVIVPIYKNDEERARVMPVVDKIRAALGSIRVKVDDRSELTPGFKF
                     NDWEMRGVPLRLEIGPKDVEKGSVMAARRDVPGREGKSFLPQENIASSVGALLDTIQS
                     SLYEKARAFRDSHITDVTNYEALTTAVQDGWAHAWWCGSADCENRVKGETKATTRCIP
                     MQQPGGTGKCAVCGRDANEKVYFARAY"
     gene            4683..5492
                     /locus_tag="P5516_04045"
     CDS             4683..5492
                     /locus_tag="P5516_04045"
                     /inference="COORDINATES: protein motif:HMM:NF020296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HRT91642.1"
                     /translation="MPAVDLSRLRARFKLLQLQIEDPVEFTRGLREIFIFHADLTHPA
                     GDSPSGVFTHPVFNTPRLLNSEVVKFLNPFVTQNPQLLLQIIDRLWREPEVELRELAA
                     QLLGKLPSSHYPEVLARIQAWSQVETDADLFPFLHQQASAAIRQQSPELWLEVLNGWI
                     SSNEPWLTKLALAGTIPLIDDYSYENLPAIFAFINPLVLNPGPQNQYELLRVIEHLAR
                     RSEVETVYFLKQVMSLSDAPGLGRFLRRAIELFSPAMQESLRVEMRQRQLT"
     gene            5630..5902
                     /gene="rpsO"
                     /locus_tag="P5516_04050"
     CDS             5630..5902
                     /gene="rpsO"
                     /locus_tag="P5516_04050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HRT91643.1"
                     /translation="MALTKEEKQKLIEEYHLHDTDTGSADVQIAILTIRIRQLTEHLR
                     ANKQDNSSRRGLLKLVGQRRRLLAYLRRTDFARFATITEKLKIRTR"
     gene            6066..8309
                     /locus_tag="P5516_04055"
     CDS             6066..8309
                     /locus_tag="P5516_04055"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HRT91644.1"
                     /translation="MKPESKKFITTVGGRPLTFETGKLAAQAGGAVTVQFGDSMVFAA
                     ATMGNEPRVGQDFFPLTVEYEERMYAGGRIPGSFFRREGRPGTDAILVARLTDRPLRP
                     LFNNAIRNEVQVIMFSLSADWENPLDVLAINAASCAVMISDIPWDGPVGAVRVGRVNG
                     EFVLNPTYSQMEESDLDLRIAGTADAILMVESGSNEIDEGSMIAALEFGHAAIQPIVE
                     TQLQMAREIGKEKRAVALYSLDETLVEKVYNRIAEEINTLLDAPHSKHELQDEIDNLR
                     ENIVLEYTDPENTEEVNAYKEAFEETYKRVVRARILEQNKRPDGRKLNEIRPIWCEVA
                     VSPRAHGSGLFTRGETQVLTMATLGTPKEAQEIDNLSPTDSKRYMHHYNFPPYSTGEV
                     KMLRGQSRREVGHGALAERALVPVIPDEKDFPYTLRLVSEVLASNGSSSMASVCGSTL
                     ALMDTGVPIKAPVAGVAMGLIKEGDRHVVLTDIQGAEDHLGDMDFKVAGTTKGITALQ
                     MDIKIKGITPEIMRQALTQAHEARLAILDKMLEVIPVPRPELRSFVPRITTLHIPVDK
                     IGAIIGPGGKIIRALQEETNTKIDIDDDGTVYIAATDGASEATVRERIEALTESAQVG
                     HIYTGKVVRVADFGAFVQILPGIDGMVHISQLDSERVERVEDVVKLGDELTVMVTAID
                     ENGKIRLSRQAVLEGWTPEEALEHDKGGKPGGNRPRSGGDRRGGSSDRRNNRGFSDRN
                     GNRNPRH"
     gene            complement(8374..9924)
                     /locus_tag="P5516_04060"
     CDS             complement(8374..9924)
                     /locus_tag="P5516_04060"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HRT91645.1"
                     /translation="MKPVLLSDLFKEFPIPWATDVAPGAIPISAIYYDSRMVRRGALF
                     VAISGGSVDGHEYISSAISRGAVAVVGTREIRGLPVPYIRVADSREALAYLAAAFFDH
                     PARQLTVIGVTGTDGKTTTANMIYEILKQAGVKAGLISTVNAVIGDEVVDTGFHVTTP
                     EAVEVQGYLSRMLAAGLTTVVLETTSHGLAQHRVTACEYDIAVVTNITHEHLDYHGTY
                     EEYRAAKGRMFASLAQTVEKPLGNPRLAILNRDDISYDYLAGLVKGRQVTYGLQHKTD
                     YFAQDVTSNPSGVDFTLIAEGLVERVHCNLPGKFNVSNCLAAFAAARAAGVETGAAIK
                     GLSRLQGVPGRMEAIEMGQPYSAIVDFAHTPNALKVALETAREMTTKRVIAVFGSAGL
                     RDRQKRRLMAEVSVQLADISIFTAEDPRTESLESILAEMADAATLAGGVEGKTFYRVA
                     DRGTAIRLGAELTAPGDLLIACGKGHEQSMCFGTIEYPWDDRTAMRATLAEQLGLPGY
                     EMPHLPTS"
     gene            complement(10001..10738)
                     /gene="atpF"
                     /locus_tag="P5516_04065"
     CDS             complement(10001..10738)
                     /gene="atpF"
                     /locus_tag="P5516_04065"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HRT91646.1"
                     /translation="MEKLGLNLGFLIVQIINFLVILVVLRAWVYTPLQNSLKKRRETI
                     EKGLEDARVAAEARENAERDAAGILAEAQNNAAQLIREAKEKAELAGREVKASVEADI
                     AKTRREAAVEVERERERVLGEIRTNVAALAIAAAQKLVGEALDEKRQHELVAEFFSGI
                     KGGKVTILEENELKGKNAEVVSALPLTRDEQKVIQGEMLSKLGETAAVSFNVDPKLLG
                     GLVVRVGDTVIDGSVAGQIQSLRATLE"
     gene            complement(10766..11002)
                     /gene="atpE"
                     /locus_tag="P5516_04070"
     CDS             complement(10766..11002)
                     /gene="atpE"
                     /locus_tag="P5516_04070"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HRT91647.1"
                     /translation="MNGDIVEAAKMIGAGIAMIGALGGGIGVGLSVQGAVQGMARNPD
                     TYGNLLSSMILGIAFSEAIAIYCLVIAFLMLFVV"
     gene            complement(11055..12020)
                     /gene="atpB"
                     /locus_tag="P5516_04075"
     CDS             complement(11055..12020)
                     /gene="atpB"
                     /locus_tag="P5516_04075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HRT91648.1"
                     /translation="MENKRKWRWGKHRWILLLLIILSVIGVNLIAPVQPHIQVAAENL
                     SEKPLFTLPVVGDFYLTNTLTAMLLMDIVIILVALGVRRAAKKNELAPGGVGSVFEML
                     VEMLYNLTENTAGKYAAKIFPWFATILIVVLFANLLKILPGFETIGFLHPSEHGFATQ
                     ALGGGWFTLKNTPAADGYVLAPFLRGVSTDLNFTTALALIAVFMIQVIGVQANGARYF
                     EKFINVRTIFKKPLFGVIDFIVGLLELISEFAKILSFTFRLFGNMFAGMVLLAVVGVM
                     LPVFMPSFIMLFELFVGVIQAFVFGMLTMVFMAQATITHNAEAEH"
     gene            complement(12010..12306)
                     /locus_tag="P5516_04080"
     CDS             complement(12010..12306)
                     /locus_tag="P5516_04080"
                     /inference="COORDINATES: protein motif:HMM:NF021073.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HRT91649.1"
                     /translation="MFDTERDNETRKTNRRNLWLAALAGQVGFLTLIIILAAVVGGLA
                     LDARLGTRPWFTIGLLLASIPVSLLLMVYITRKTVAKIKANHNDHLNEEGRLGK"
     gene            complement(12372..13820)
                     /locus_tag="P5516_04085"
     CDS             complement(12372..13820)
                     /locus_tag="P5516_04085"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HRT91650.1"
                     /translation="MPAITAEMLLSLLPELILALLALFLLALSLFKKENLGGAYSWVT
                     AFGFGLTLFLTVFYSRPPAEVQLLWGEMIRFDRVGFIFRLLILAGGAMTAIFAGMEQT
                     IAKKAEFYLLLVLSSIGFSLMVSAGNLIMLYLAIETATIPLYVLAGFKQRDEKSVESG
                     IKYFLFGGVSSAILLYGFTLLYGFAGTTRLDGMLELIKQNGAPIAGITLASLLVLAGF
                     AFKMSAAPFHFWAPDVYEGSPTPVAGFLSTVSKAVGFGAFIRVLVNAFGVGSSNAWMV
                     IVAILAAASMFLGNLLAISQRDIKRLIAYSSIAQAGYMLVGVAAGTEFGFSGTVYYLL
                     VYLVTNLAVFAIISWVEKGSGSSAVSAFAGLNRRSPGMALVMMVALLSLGGIPPFGGF
                     FAKVLVFGAAVQSHYVWLAVLGILNSVIALYYYLKIMKVMYLDKPDETSWKVTPALQW
                     RVALALCIACIILLGVIYAPWLNGISLAVTGF"
     gene            complement(13820..15292)
                     /locus_tag="P5516_04090"
     CDS             complement(13820..15292)
                     /locus_tag="P5516_04090"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRT91651.1"
                     /translation="MPVPLLSILALTPLAAGIIVLLLPAEKRSLIRGIAFATSLFTLA
                     VSVIIFFRFDTTAGGYQFIEKMNWLPALGISYHMGVDGIGAAMELMAGIVVFSGVMVS
                     WRVEDRPREFFAFLLFLAASVFGVFASLDLFQLFFFFELAVFPKYLMIVMWGSPKTRE
                     YGGMKLTLYLFLGSVVALIGVLAVYFTSGLRSFDMLALEQAGLALEFQRAWFLVIFVG
                     FGVLGGLFPFHSWAPDGHVAAPTAVSMLLAGVEMKVGVFAALRAGLMLLPEGMRYWAP
                     LILVLATINVVYGAFIAMIQKDFKYVIGFSSVSHMGLVFIGFATLTREGLTGAGLQMF
                     SHGIMTALFFAVVGMVYDRTHTRDIRELGGLFRTMPIAMLGFVIGGLVSMGMPGFSGF
                     IAEMPIYMGAWQTAPIVAIISALSIVITAAYILLVVRRVFFGETPEGIRAGLSDISLM
                     DKIVVVLLSLILIVIGLFPSVLVPWIEAGVRHILVLTGGA"
     gene            complement(15303..16838)
                     /locus_tag="P5516_04095"
     CDS             complement(15303..16838)
                     /locus_tag="P5516_04095"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HRT91652.1"
                     /translation="MNILSAHLLSLILFLPLVGAVVLLAFPANKVKAIRGVALVASLI
                     PLALTLVLWGSYSTALTVGEFKFVENYPWFTSIGSSYHLGIDGVSLVMLLLTTLLTPL
                     AIMTSFNIKERVKAFMILFLVLETGMLGVFMALDLVLFFLFWEIGLVPMYFLINQWGG
                     EKRNYASLKFILFTMGGSLGLLLAIQMIGVVCGTFDLVTITASWPALQGGELFGIPIA
                     TVKAIAFWAFVVAFAIKVPVWPFHTWLPDAHTEAPTGGSMVLAGVLLKLGGYGFIRLV
                     LPLFPAESARFASWLAALATAAIIFGALAAAAQTDFKRLVAYSSINHMGFVVLGIAAA
                     AWAGGQTNPTLRESAVIALNGAVLQMFNHGLTAAGMFFLVGVLYDRAHTRQLAEYGGL
                     FAILPVYGAILVFISMASLGLPGLNGFVSEFMIVRGVWPIFTLFIALAMIGLLFTAVY
                     VLKALKLTLHGALNEKWVGKLTEISRRELVVIIPLMLLVVIIGWWPAWILNLINQTVM
                     TWF"
     gene            complement(16835..19033)
                     /gene="nuoL"
                     /locus_tag="P5516_04100"
     CDS             complement(16835..19033)
                     /gene="nuoL"
                     /locus_tag="P5516_04100"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HRT91653.1"
                     /translation="MFNIHPLIWLIPLPPLLGFLLIVLFTRRSHRASHSVALAGAGLS
                     FVGSMVVFIKAVTAGGVADSSFAASINWLPTGASWLRIGVLVDPLTAVMLFFVAWTVL
                     MIFIYSIGYHNFGQPIGKQDRPGLPPQGAEITEAGKIHRVPSVEPMYARFFALLSLFA
                     FAMYLLVVSDNLLTLYIGWEIMGFCSYFLIGFWYGKESARKAGIKAFITTRIGDVFML
                     LGMVALYSVTGTLNFHEIFSNPETMTLLTSTLSPVLGLNWAGLIGLLLFCGTIGKSAQ
                     FPLHVWLPDAMEGPTPVSAMIHAATMVSAGVYLSARMFPLMSAGWHHGASLTVPMMAM
                     ALIGGFTALFTSTIALAQNDSKRVLAYSTISQLGYMIAALGIGAFTAAVFHLVTHAFF
                     KALLFLGSGSVIHGMEHGALHTGKHIDAQDMRHMGGLRKKMPVTFWTFLAGGLALSGF
                     PLITAGFWSKDEILSGAFGGGHLALFIVLAVTALLTAFYTARQITLVFLSKPRSDAAA
                     YAHETKPVMTAPLVVLAVFAVTFGWVGIPHAFPGLGAISPAWFQSFVGSMLSPGSVSE
                     AHSNIPLVVSILVSLGGLLLGWLVYRGYESAQEPDPLQKRFGGVFRLLEHKYWIDELY
                     QKVVINPVLWLADRVIYQFIDLRLIDGFLHGLAAAGVWLGNLLRSKFDLPVVNGAGDN
                     LAGGTRGLGDFLRRLQTGRVQQYLAIAVACTVVAALIILSYLAKVQAGSG"
     gene            complement(19046..19360)
                     /gene="nuoK"
                     /locus_tag="P5516_04105"
     CDS             complement(19046..19360)
                     /gene="nuoK"
                     /locus_tag="P5516_04105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HRT91654.1"
                     /translation="MIPLYWFLVLSAFLFSLGLFAVITRRNTIAILMGIELMLNAANI
                     NLLSFWRYLHAPRGDMDGLIFALIVFVVAAAEVAVGLGLIVSAYRRRRTVVANELDLL
                     KG"
     gene            complement(19357..19875)
                     /locus_tag="P5516_04110"
     CDS             complement(19357..19875)
                     /locus_tag="P5516_04110"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HRT91655.1"
                     /translation="MTGMQIVFLINAAVTVIAGVMMVTVKRLMHAAMWLILTLFGVAV
                     VFGLLEASFFAIVQVVVYIGAIAILLVFAIMLTRDAVDDSFLQNHRWVLTALIGAVAL
                     SGLLLAIFTWPQSALFTAPLADAQRDVGEMGLSLLDPQGYLIPFEVSSILLLAALIGA
                     VHVGISRREEER"
     gene            complement(19872..21104)
                     /gene="nuoH"
                     /locus_tag="P5516_04115"
     CDS             complement(19872..21104)
                     /gene="nuoH"
                     /locus_tag="P5516_04115"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HRT91656.1"
                     /translation="MSFFADPITFISNWLNSVLSNWGVPPGWAQFMLFILGAGALSMG
                     AMVFTIFLIWLERKLGGRFQDRIGPNRVGPWGIFQTIADMLKIFTKEYITPWGSDPVL
                     FNLAPVLAMAAVLAVWAVIPLSSTVYGSNIQIGVLYILAVGALGELGIILAGFASNNK
                     YALLGGFRVVAMLISYEVPMALALLVPVMLTGSMGLNDITSAQRVWFIWKAPVAALVF
                     FIASIAENSRAPFDLIEADSEIVAGFNIEYSGLKFGMFYVADFLHAFTAASIFASVFL
                     GGWRGPGAELYPALGFLYLLIKTAIVYFITISIRFSLPRLRVDQVMAFNWKYLTPLAL
                     VSLLVTALVDKSLPAGQGWLRLGGLLLANLVIFVVADRLVSIYHRRKNQERRVVSGPR
                     PIAVTLREKPAEPVEVES"
     gene            complement(21127..21483)
                     /locus_tag="P5516_04120"
     CDS             complement(21127..21483)
                     /locus_tag="P5516_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HRT91657.1"
                     /translation="MLHEWLYLVIFLTISMFIPAAAIGAAALLSPKKPSPLKNSIYEC
                     GIETVGPSRIQFKAQYYLYGLLFLIFDVETVLLYPFAVAYNRLTLFAVLEAVLFVAIL
                     AAALVYAWRKGALEWT"
     gene            complement(21555..21890)
                     /locus_tag="P5516_04125"
     CDS             complement(21555..21890)
                     /locus_tag="P5516_04125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91658.1"
                     /translation="MNLELAARLEAGFEELAPAGRVTLHLRSGSEVGLIWKLRTGEEA
                     VYPATLWIWVTSDAADELLLAREFTLQSRGFMGLAVRPVRISAGFLAASGLLVVGYGQ
                     TLRCRLKQY"
     gene            complement(22180..22809)
                     /locus_tag="P5516_04130"
     CDS             complement(22180..22809)
                     /locus_tag="P5516_04130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91659.1"
                     /translation="MTTDESKLEILRKVEDGTLTPEEGSELIGILDAAGAEESEREFI
                     RPPLEPETPGADQETLRVSGCWKAAWSMILLGGAVLTAFSADWIYLGYQKAGLGWSFW
                     LSWIPLAIGVLIMVLGWILMTSPWLQVRIRTHEKGRLQNIILNIPLPLSLVSWVFSNF
                     GQCMPDAVKEKGVEEMLLEIGQALKQNEPFQVEVDDQQDGDHVFICITR"
     gene            complement(22822..24024)
                     /locus_tag="P5516_04135"
     CDS             complement(22822..24024)
                     /locus_tag="P5516_04135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91660.1"
                     /translation="MKQVSMPIKTDSQVNIIVSDDLSLSGKNQLLMTALVKESDSLKF
                     SEDQGKVEIRATSDLSLQLPALQPVTVEKAGGDAHVSGLIQRVIIGKVGGDLALVDLA
                     GASVEMVGGDISLQRTTGAVEIARVGGDLVGSAVESVLSSAVGGDIYLRDVKGSLNLV
                     AGGDVVLQLASANPAMVTITAGGDVKIRVHAACNAQLELYTEGGSIRLNACGQQGEWD
                     QEQVVIPFGEGGNVVSIKAEGDILIIDHDDLDGVFQSRWDEMFENWEGFGSDLEKRVR
                     ESVAATSERIKWATLGTAAAGEKVRLKLEQAMRKLDERGKRLSERGITIDLPEKPGVC
                     GDINATGFRRGENDKVVGFTFPGENASTPTPTSKATDEERVMVLKMLQEKKITLEEAE
                     KILSALEK"
     gene            24253..24675
                     /locus_tag="P5516_04140"
     CDS             24253..24675
                     /locus_tag="P5516_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91661.1"
                     /translation="MKNITVSTAIVLLLCFLLITGCAQKQAVTYLSADESAQVATYTD
                     VAAQNIINAIAANDYNLFISDFDEQMRHALPEEQFATIVKMYVKNGQAESISLLNMED
                     RGDFIGANYGVTYSNAALTMLIVVAKSDPSLVSGLWFK"
     gene            24763..24927
                     /locus_tag="P5516_04145"
     CDS             24763..24927
                     /locus_tag="P5516_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91662.1"
                     /translation="MKLTPPKNITWVIALVVGVLGIIGHLAGFFGDFAFWLVVIGFVI
                     LVLSTLLKDL"
     gene            complement(25012..>26052)
                     /locus_tag="P5516_04150"
     CDS             complement(25012..>26052)
                     /locus_tag="P5516_04150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91663.1"
                     /translation="PWVRPDAIAYALLILFWDCVKRKRVLWAGLTAVVIGVSSLLIFN
                     SLYFGTLLQQSINAKLLMRHPFTISRFADNLFTVFVGNAGGAFSPYRAEIFGRAGILF
                     GLLVLLAMGLYLGRVWSDRAPRITGLALASITWLVPVAYAFGGVLYQWYTWPPAIFGS
                     VFLLALIVEWSTGTGKWRQVVRWGCVLLMLAGLNAQMVFSYVWGTKEFAYRGGIGVWL
                     KEHAQAGDKMLLEPAGYIPYYSGLYTYDEVGLVSPQVVNYRLAYEERWWPEFVMDYRP
                     DWLVQREHIKHDKTYQGYTLNGEELEWLRDNYRLVAWFSYQPEDYTSSHFLASLLSLA
                     EADDYYIFKRIE"
     assembly_gap    26053..26152
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<26153..26370)
                     /locus_tag="P5516_04155"
     CDS             complement(<26153..26370)
                     /locus_tag="P5516_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91664.1"
                     /translation="MTRRILQQPAARFALLFLLLLAARVPFYFTHHIQEDAYISLRCA
                     ENLAQSGVYGFNPGERVSASTSHLYVFLA"
     gene            complement(26367..27812)
                     /locus_tag="P5516_04160"
     CDS             complement(26367..27812)
                     /locus_tag="P5516_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91665.1"
                     /translation="MRKMALVIALLFILSAIAPARADIAPPTYPPGFNPAPGSEQTQV
                     RMESESVRLNIREADGGEEMGFADVQALFNMKNLSSTDERMAVRFPAAVGNGWFGVTP
                     VQDISVKVNGTPSNTRRISGEDPNGFEKAVDWVEFDVLFPAGQPVKIEVAYTLEASGE
                     MPYIWFQYIFSTGAGWKGTIGSADLIVNLPYQVDELFLLPCIDDATNCTTPGWVKEGE
                     TLSWQYRDFEPKPEDNFTISLVAPSVWKQVLRERARVAAAPKDGEAWGRLGKLYKTLL
                     FSPHGRRGFRTWHFQADPGVKELFELSDEAYTKATDLLPQDALWHAGYADLLAYYAVY
                     AGYEGEQTLPLKLKALEQIHLALQLAPDDETVKGIAVDLTWLMEEGIVEVDERFDFPW
                     LTQTPVLTAVQFEPLLDGVTPTLQPLITKTSSPPVAASAAALQTAAAESPEKDLNRRP
                     GTPFCGGALLIPLLLVTGAKLFQMRKDRDSD"
     gene            complement(27904..28653)
                     /locus_tag="P5516_04165"
     CDS             complement(27904..28653)
                     /locus_tag="P5516_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PmeII family type II restriction endonuclease"
                     /protein_id="HRT91666.1"
                     /translation="MNNLSLEDVKSYVHEHIGEFHQDRINSLSALKLTKVLQRKNPYL
                     YKAKNMLTSEMIVRGFVDSHISSNEETIFGTWLEGLAIFINSQVFNGWKSGIPGIDLE
                     FDYEGIRYIVNIKSGPNWGNSSQISKLIDDFSRAKRTLRTSNSKLSITAVNGCCYGRD
                     NKPDKGEYYKFCGQKFWSFISGEQNLYTDLIEPLGFEAKKRNEEYSELYVNMINRFSM
                     EFNRYFCQENGAINWVKLIEYNSGENTFELI"
     gene            complement(28646..29629)
                     /locus_tag="P5516_04170"
     CDS             complement(28646..29629)
                     /locus_tag="P5516_04170"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRT91667.1"
                     /translation="MDMDDSCVVYRLNKGVSPNLQDSGGLYIINSQQIPLFIEPAVDS
                     PLVVEGYKNSIINGDCELILKGLPDNSVDLIFTSPPYADQRTSTYGGIKPEKYVNWFL
                     PKAKEFYRILKPEGSFILNIKERVVNGERHTYVIELILELRRHGWYWTEEYMWHKKNS
                     HPGKWPNRFRDNWERLLHFTKQKKFAMYQEEVMVPVGDWAQTRLKNLSTTDKKRDNSR
                     VGSGFGKKIENWVGRDLVYPSNVLYMATECYNQQHSAVFPVALPEWFIRLFTKPGDLV
                     LDPFNGSGTTCVAAALLDRMYTGIEINNEYCEVAINRLKAELIKKERKQDE"
     assembly_gap    29902..30001
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(30036..30113)
                     /locus_tag="P5516_04175"
     tRNA            complement(30036..30113)
                     /locus_tag="P5516_04175"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(30076..30078),aa:Pro,seq:cgg)
     gene            30194..30997
                     /locus_tag="P5516_04180"
     CDS             30194..30997
                     /locus_tag="P5516_04180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91668.1"
                     /translation="MKRPSLPLLLFICWLLAACAAPATPAVLPPTFTIQTAAIASTSL
                     PSPTSAPTSTATPATPAPPAVTLPQGALSAQQRQALENAARAFISPSPEEALATAIEL
                     GYLGRNANPATMCGPLALAILQQSGLVDSSFPLKSFYYLNPRPGHDERYLEKVFPADR
                     FEKIVREESIGKIDFSADPLLPGDFLYLFAGDSGSFEHMLVVSRVDDAGRAYAVTNLN
                     TARGVIVDEVLLYDPANPGVGQFYQWTDWENRMIGRTGYGGYWLWRPLP"
     gene            31090..31449
                     /locus_tag="P5516_04185"
     CDS             31090..31449
                     /locus_tag="P5516_04185"
                     /inference="COORDINATES: protein motif:HMM:NF023395.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HRT91669.1"
                     /translation="MTSCVFCDIISGSAPSDQLYADEELLVIQDIHPVAPVHVLVIPR
                     KHIPSLNHLQPEDQALASRLLLTVPRIAHQLLGDDAAYRTIINTGAAAGQTVFHLHVH
                     IISGRPFINHLLTRGLY"
     gene            31449..32975
                     /locus_tag="P5516_04190"
     CDS             31449..32975
                     /locus_tag="P5516_04190"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT91670.1"
                     /translation="MHQSARLLFAILATLLISGCIKPAPGSVPWQISGTQELPGAQHP
                     VTSSPPSWMPATRVPGSPYFTPTPDTPRVMPTLRADQEFYTVQYGDSLGNIALLYNLD
                     LASLIAANDLANPDILEIGQVITIPAPQPGAEPSSFKIIPDSELVYGPVSSTLDIEAF
                     VNERNGYLNAYREEVEKIEMSGAQIVARVSREFSINPRLLLALLEYRSGWVTTSLIDD
                     SLRDYPMGLQDPARKGLYLQLAWAAKLLNRGYYLWKINAISFTSFANHSLALLSPTIN
                     AGTAAVQYMLGVGSYPDAWQRAVSEEGLFRTYCELFGIPFDLAFEPLIPADLQQPALT
                     LPFEPGTLWSFTSGPHAGWADGSAWAALDFAPPGEQLGCYITDEWVTAVADGLIVRSS
                     SGAVVLDLDGDGLEQTGWTILYMHIETRDRVAAGARVKTGDRIGHPSCEGGFSNGAHV
                     HIARRYNGEWISADREIPFNMSGWVSSGDGSEYSGTLTKNGKVVYSWNGRVDANQIGY
                     "
     gene            32953..34548
                     /locus_tag="P5516_04195"
     CDS             32953..34548
                     /locus_tag="P5516_04195"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRT91671.1"
                     /translation="MRTKSVTRLFQIVAAWAVLAGSMLACSQGYVSPAELTATAGLRP
                     MADTTAAPTEETPFTANISLFQTPTQPPLPTFTDLPAFTSTPQPSRTPDPNATPKPPL
                     QYYTQAGDTLASITGRFGVSADQIIPSEPIPVDGLINPGVLLIIPDVLEDVYESLTLL
                     PDSEVVYSPSAVNFDILKYVAEADGYLNRYQETVGSRQYSGAELVQRVALENSINPYL
                     LLTLLEYKSHWVSSRPTNMAESEYPMGYVRLEYRGLYKQLSWAVEQLSIGYYGWRAGS
                     LNSLTFKDGSSVRISPGLNAGTAAIHYLFSRWYNQAEWAAALYGADNMPNLMSHMFGD
                     LWERSRAVDPLYPADLQQPEFSLPFQPGRVWSFTGGPHSAWGEGGALAALDFAPPSST
                     AGCVASNEWVTAAASGVIVRAADGIVIQDLDGDGVEQTGWNIMYLHVETRDRVQPGTY
                     VQTGDKIGHPSCEGGISTGTHVHVARKFNGEWILADGPLPFTLSGYVAKNGGSPYQGS
                     LESGQARIIANPLGTLRSYISLP"
     gene            34651..>35915
                     /locus_tag="P5516_04200"
     CDS             34651..>35915
                     /locus_tag="P5516_04200"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT91672.1"
                     /translation="MSILILSACSPQPPITVYITPFPLSATEAPAQQPTALATRPVYQ
                     PGTLVAYTARTGDNLPALAHHFNTTVKEILENNPQLSEKTTTLTPGENLQIPIYYEAL
                     WGNPFQIIPDSLYINGPAQIGFDTKSFVNAHPGWLKNYASLAGGEVRRGADLIDYVSL
                     EYSISPRLLLALAEYQAGALSVETLEPQEKVYPLGYEDEFHQGFYLQLLWAANTLNNG
                     YYSWRSGKLGTITRLDGSIEHPDPWQNAATVALQNYFSQVLPVADYHKAIYSEGLYNT
                     YAALFGDPWQHTSDHIPGDLQQPALRLPFVPGKTWSYTGGPHSAWGEGAPLAALDFAP
                     PSVVGGCAASNEFVTAMADGQIVRSEKGLALLDLDGDGDEHTGWVILYLHLGLNDKIR
                     PGMLVKTGDLIGHPSCEGGSATGTHVHIAR"
BASE COUNT         9002 a        10104 c         8847 g         7762 t
ORIGIN      
        1 ttgtctttga cagaaaaagc atcgtccggt agaattatgc ggttattaca tcatctatta
       61 acagaaggac ccggtccatg aaagaatatt caactgaaaa aattcgcaat attgtgctgg
      121 catcacactc tggtgctggg aagaccatcc tgtgcgaagc tttcctccat gccaccgggg
      181 caacaacccg tatgggcagg atcgaagacg gcactaccgt ttctgatttt gacgaagaag
      241 aaattcgacg cacgatttct ctttccacat ctgtgattcc tgttgagtat cgtgatgtga
      301 agatcaatgt attggataca cccggctaca ctgactttat cggtgaagtg atctccgcgc
      361 tgcgcgtggc agatggcgcg gtcctgctga ttgatgcggt caacgggctt gaggttggca
      421 ctgaaatcgc ctgggattac tgcaccaggt tcaaccttcc gcgctttttg ctggtaaaca
      481 agatggaccg cgagaacgct aattttcgca aagcgctgca aacagtcgaa gattattctg
      541 aaacccgcct catacctatc cagctgccct ggggtgaaaa gcaagacttc aagggtgtga
      601 ttgacctgct ctcgatgaag gcgtataaag cgcaagggaa aacggcggaa gaaattcctg
      661 cggatatgaa agacgctgcc gaagaggcgc ggatgaaact ggtagaagcc gccgccgaag
      721 gagatgatgt tctgcttgaa aagtatctgg aaagcggcag cctgacagaa gaagaggtca
      781 tcagagggct cacactggtc gttcaatccg gagatttcgt tcctgttctg gtctcctcgg
      841 gttctaatga aatcggcgtc atccgcctgc tggatgccat ggtagacatc atgccatctc
      901 cgctttccgc ggcgaaaatt gtggcgcaag gcaaagccgg cgaagaaatc ctcgaaccaa
      961 aagataacgc tccgcttgcc atttacgtct ggaagaccac cgccgacccg ttcgttggca
     1021 agcaaacctt cttgcgcgtg tacgcaggaa ccatcagccc tgatgcgcgg gtttggaatc
     1081 aaaacaaggg catcgaagaa cggttggggt cggtgaacgt tcctcgcggt aaagaatctc
     1141 tgccggttaa agtgatccac gcgggtgata tttgtgtcgt gccaaaattg actgaaactt
     1201 ccaccaccca cacgctgtgc gataaaaacc atcccctcac cctgcctgtt cctgaatacc
     1261 ccacctccct ttaccaggtt gccctttcac ccaaaacgca ggcggattcc accaagattt
     1321 cttcttctct caccaggttg tgcgaggaag atatgacgct ctcctggcac ttccagaccg
     1381 ccacgaaaca aaccatttta cagggcatgg gcgaccagca tatcgatgtc gccattcgcc
     1441 gcgctgaaag caagttccag gttgggattc tgatggaaga acccaaagtt ccctaccagg
     1501 aaaccatcac caagagcgct tctgccatgt accgccacaa gaaacaaacc ggcggttccg
     1561 gtcagtttgg cgaagtgcac ctcaaggtct ctccgctgac tgataaagaa ttcgatttca
     1621 cctgggatgt attcggcggc gcgatttcgc aatcctattc ctcctccatc caaaaaggca
     1681 ttcagggcgt gatgaaggaa ggcgtcattg ccgggtatcc ggtttcaggc gtgctggtat
     1741 ccgtgtatga cggcaaagaa cacccggtgg attcaaaacc agtcgccttc gaagttgccg
     1801 ggcgtgaagc attcaaactg gctattaaag acgcggcccc ggtgctgctg gaacctatca
     1861 tgctcgtgaa aatcacagtg cctgaagccg ccatgggcga tgttatgggt gacctgaaca
     1921 ctcgccgcgc gcgtatccag ggaatggaaa cgcaaaaagg tcgttcggtg gttaccgcga
     1981 atgtaccgtt gacggaaatg ctgcgttaca ccaccacgct tcgctctatg acggggggtc
     2041 ggggtgtgtt ctcgatggaa ttcgaccata ccgaggtggt gcccacccat cttgcccagg
     2101 cagtgatcga tgcccacacc aaagaaatgg aaagccgcaa agaagaataa gcgcgcacct
     2161 tgtaaaaagc tggacggaaa acacccgtcc ggctttttat tttccgctgt tccttttttt
     2221 gtaggaaaag gaacctctat ggtaatattg ggtgagttcc acatagtgag tcaagcatga
     2281 agagagcgat aattcaagag ttgtgcgcca ggaagttcaa ccggatagtc aagaaggctg
     2341 aaggataaca tggatatcag ccagctcaca acggagatga accgctttgt cagcgccaaa
     2401 ggctggtacg ctgaaaacag cccgcgcccg caaaccgcgc gcaacctagc aatctcgctg
     2461 gtgctggaag cagctgaagt attggagctt tatcaatggg gagagtctgc cgtttcaaac
     2521 caggagttga gcgacgaatt ggcggatgtc gccctgtatc ttctgcagat cgccagcatc
     2581 accgggattg acctggaaga agcgattatg gataagctga agaagaattt tcagcggtca
     2641 tgggacggcg cgggaaccga aaggaaggaa atcaaatgaa tatcgcggta tttggcggag
     2701 cttctgtccg cccagatgac gtggtttata ctgagacgtt aaggctggga aaaatgttgg
     2761 gcgggcacgg gttcaccgtc ctgacaggtt cttacatcgg cgtgatggag gctgcctcgc
     2821 gcggcgccaa tgaagccggt ggtcatgtgg taggaatgtc ctgcgctgaa attgaaaact
     2881 ggcgccaggt gaagcccaat cgttgggttt tagaagaaca tcattacgaa accttacgcg
     2941 aacgactctt cgcgctcatc gatggctgcg atgcggcgat tgtgatgccg ggcggcgtag
     3001 gtacactgag cgaactggta gtgatgtgga acgaaatgat tatcaacagt aaacccccca
     3061 gacctctcat cgttgttggc agcgcgtggc agcgtgtgat ggcacaattt ttcgcatcct
     3121 tgggagatta catcggtgtt aaagaccgcg aattgatcca gctggtgcca gacgtagaca
     3181 ccgccgtaga aatactcgtg gattatcaaa atcaatctat aagggaaagc ttatgagcaa
     3241 cgaaaaacta ccttcgcgcg cagaaaatta ttctgaatgg tacaaccagg tcattcaacg
     3301 ggctgaattg gctgattatg ccccggttcg tggctgcatg gttgtgcgcc cttatggatg
     3361 gtcattatgg gaaaacatcc agcaggccct ggacagccgt tttaaagcca ccggtcatgt
     3421 caatgctgct ttcccgctct ttatccccat gtcatttctt gaaaaagaaa aagagcacgt
     3481 agagggcttt tcgcctgaac tggctgtagt gaccatcggc ggcggcgaaa aacttgaaga
     3541 accgctggtg gtgcgcccaa cctctgaaac gatcatcggt tacatgtact ccaagtggat
     3601 taaaagctac cgggatctgc cgctgcttat caaccagtgg ggcaacgtgg tgcgctggga
     3661 gatgcgcaca aggctcttcc tgcgtaccct ggaattctat tggcaggagg ggcacaccgc
     3721 ccacgccacg gcaaaagaag ccgaagaaga gacactgcgc atgctggata tttatacgcg
     3781 tttcgccgtt gaagatgccg cggtgccagt gatacccggg cgaaaaagca acagcgaaaa
     3841 attcgctggc gcggtgcact cttattccat tgaagctatg atgggcgata ccaaagcatt
     3901 gcaggcgggg acatctcacc tgctggggca aaacttcgcc aaagccttca atatccagta
     3961 tctggatatg aacaatgaat tgcaatactg ctggacgacc tcctgggggg tttctactcg
     4021 ttttatcggc gccatcatca tgacccatgg cgatgaccag gggttgatct tgccgccccg
     4081 cctggcgccc atccaggtgg tcatcgtccc catttacaag aacgacgagg agcgcgccag
     4141 ggtcatgcct gtggttgata agatccgcgc tgccttaggt tcgatcaggg ttaaagtgga
     4201 tgatcgtagc gagttgactc ccggcttcaa gttcaacgac tgggaaatgc gcggcgttcc
     4261 gttgcggctt gaaattgggc ccaaagatgt ggaaaaagga tctgttatgg ctgcccgacg
     4321 cgatgtgccc ggtagagagg ggaaatcctt cctcccgcag gaaaacatcg ccagctcagt
     4381 aggtgctttg ctggatacta tccaatcctc tctctatgag aaggccaggg ctttccgcga
     4441 cagccacatt acggatgtga ccaattatga agcgttgaca accgctgttc aagatggctg
     4501 ggctcacgcc tggtggtgcg gcagcgcaga ttgtgaaaac cgggtcaaag gagaaacaaa
     4561 agccaccacc cgctgtatcc ctatgcaaca accgggtgga acaggtaaat gcgcggtgtg
     4621 tggccgcgac gccaatgaaa aggtgtactt cgcccgcgct tattaagcag ggagcctgaa
     4681 ccatgcctgc cgtcgatctc tcccggctgc gcgccaggtt caaacttctc caattgcaaa
     4741 tagaagaccc tgttgagttc acacgcggtc tacgcgagat ttttattttc catgccgatc
     4801 tgacccatcc agcgggcgat tctccctcgg gcgtgttcac ccacccggtc ttcaatacac
     4861 ccaggttgtt gaacagcgag gtggtgaaat ttttaaatcc tttcgttacg caaaacccgc
     4921 agctgttgct ccagatcatc gaccggcttt ggcgtgaacc cgaagttgag ttacgagagc
     4981 tggcagccca gttgcttgga aaacttccca gttcgcatta tccagaagtt ttggcgcgta
     5041 tccaggcatg gtcacaggtt gaaacggatg ccgacctgtt ccccttcctg caccagcagg
     5101 cttctgctgc catccgtcag caatctcctg aattatggtt ggaggtcttg aacggttgga
     5161 tttcgtctaa tgaaccatgg ctgacgaaac tggcgctcgc cggcaccatt ccgttgatcg
     5221 atgattacag ttacgagaat ctgccagcga tttttgcttt catcaaccct ttggttttga
     5281 acccgggacc acagaaccag tacgagctgc tgcgagtgat cgagcatctt gcccgcaggt
     5341 cagaagttga aactgtttac ttcctcaagc aggtaatgtc cctttctgac gcgcctggct
     5401 tggggcgatt cctgagacgg gcgatcgagc tgttcagccc tgccatgcag gagtcgctgc
     5461 gtgtggagat gcggcagcgt cagttaacct agaaaaaggc actttcgttt cagaaatttg
     5521 tgtctgtgca ccaataaaac cctctggtat aatacgcctc attgactcga cctgttggcg
     5581 gtttgggctg ccagctggcg acttgcaaaa tacttaagga gcaactgtca tggcattaac
     5641 gaaagaagaa aaacagaaat tgatcgaaga gtaccacctg cacgatacgg ataccggttc
     5701 agcagatgtt caaatcgcga tcctcactat ccgcatacgg caattgaccg agcacctgcg
     5761 cgcgaacaag caagataatt cctctcggcg cggcttgctc aaactggtag ggcagcgccg
     5821 ccggctgctg gcatacttac gccgcactga ttttgcccgc ttcgctacca ttaccgaaaa
     5881 attgaagatc cgcaccaggt agattgagcc aaaacgagta gaacccctct actcgtttta
     5941 aatgcacatc cagggcggaa caacctagca ccgccggttg ggtattgaaa agtgaagaag
     6001 acgtccgcgc gtcttcccca gttttcagtt cctaaccagg cgggaaaaga acattaggag
     6061 aaattatgaa accagaaagc aagaaattta ttaccaccgt tggtgggcgt cctttgacgt
     6121 tcgaaaccgg taaactcgcc gcccaggctg gcggcgctgt gaccgttcag tttggcgaca
     6181 gcatggtctt tgctgcagcc accatgggca atgagccccg tgtcggtcag gatttcttcc
     6241 cgttgaccgt ggaatacgaa gagcgcatgt acgctggcgg gcgtatcccg ggttcattct
     6301 tccgccgcga aggccgaccg gggaccgatg ccatcctggt tgcccgcctt accgatcgcc
     6361 cgctgcgccc gctgttcaat aacgccatcc gcaacgaagt tcaggtcatc atgttttcgc
     6421 tctccgctga ttgggaaaac ccgctggatg tgttggcaat taacgccgcc tcctgcgcgg
     6481 tgatgatctc tgatattccc tgggatgggc cggttggcgc ggtgcgtgtt ggtcgtgtca
     6541 atggtgagtt cgtgctcaac ccaacttaca gccagatgga agaatctgac cttgatttgc
     6601 gcatcgccgg taccgctgat gccatcctca tggtcgaatc tggttcaaat gagattgatg
     6661 aaggctccat gatcgccgcg ctggaattcg gtcatgccgc tattcaacca atcgttgaaa
     6721 cccaactgca aatggcgcgc gaaatcggca aagaaaaacg cgcagtcgcg ctctattcgc
     6781 tggatgaaac cttggtggaa aaggtttaca accgcatcgc ggaagagatc aatacactcc
     6841 tcgacgcccc gcacagcaaa cacgaattac aggacgagat tgacaacctg cgcgagaata
     6901 tcgtcttgga atatactgac cctgaaaaca ctgaagaggt caacgcgtat aaagaagcgt
     6961 ttgaagaaac ctacaagcgc gttgtgcgag cgcgtatcct tgagcaaaac aaacgcccag
     7021 acgggcgcaa attgaatgaa atccgcccga tctggtgcga agtcgccgtc agtccccgcg
     7081 ctcacggctc gggtcttttc acccgcggcg agacccaggt gctcacaatg gccaccctgg
     7141 gcacacccaa agaagcgcaa gagattgaca acctttctcc cacggactca aaacgttaca
     7201 tgcaccatta caattttccg ccgtactcca ccggtgaagt gaaaatgctg cgcgggcagt
     7261 cccgccgcga ggtaggtcat ggcgcactgg cagaaagagc gctcgttcct gtaattcccg
     7321 atgaaaaaga tttcccctat acgctgaggt tggtatctga ggtgctcgcc tccaacggct
     7381 catcttccat ggcttcagtt tgcggctcaa ccctggcatt aatggatacg ggcgtaccca
     7441 tcaaagcgcc ggttgccggt gtggcgatgg ggcttatcaa agaaggcgac cgtcatgtgg
     7501 tgctcaccga tattcagggc gctgaagacc atctgggcga catggatttt aaagttgccg
     7561 gaaccaccaa gggcatcacc gccttgcaaa tggatattaa gatcaagggt atcaccccag
     7621 agatcatgcg ccaggcactc actcaggctc atgaagcccg ccttgctatc ctggataaaa
     7681 tgcttgaagt catcccggtt ccacgaccag aactgcgttc cttcgtgccg cgcatcacca
     7741 ccctccatat acccgtggat aaaataggtg ccatcatcgg gccaggaggt aagattatcc
     7801 gcgcgctgca ggaagagacg aacaccaaaa ttgacatcga tgacgatggc actgtctata
     7861 tcgccgctac tgatggggcc agcgaagcca ccgtccgtga gcgtattgag gcgctcaccg
     7921 aaagcgccca ggtcgggcat atttataccg gtaaggtcgt gcgtgtggca gatttcggtg
     7981 cgtttgtaca aatactgcca ggtatcgacg gcatggtgca tatctcgcaa ctggatagcg
     8041 aacgcgtaga acgggtcgag gatgtggtca agctgggcga tgagctcacc gtaatggtca
     8101 ccgccatcga cgaaaacggc aagatccgct tatctcgcca ggcggtgttg gaaggatgga
     8161 caccggaaga agccctcgag cacgataaag gcggaaaacc tggcggcaac aggccgcgct
     8221 ccggtggtga tcgtcgcggt ggtagcagcg accgcagaaa caatcgtggt tttagcgacc
     8281 gcaacgggaa tcgcaatccc aggcactaag gctgtaagca tataagaggt ccggtttgcc
     8341 aaccggacct tttttcttta cacagaaaaa tcgttatgaa gtaggtagat ggggcatttc
     8401 gtagccaggc agaccaagtt gctctgccag cgtggcgcgc atggcagtac ggtcatccca
     8461 ggggtattcg atcgtgccaa aacacatcga ttgttcatgc ccttttccac aggcaatcaa
     8521 caggtcgcct ggcgcggtaa gttccgcccc cagccggatg gccgtgccgc ggtcagccac
     8581 gcgatagaac gtttttcctt cgacgccccc agccaaagta gctgcgtccg ccatttccgc
     8641 caatatactc tccaacgatt ccgtccgcgg atcttcagcg gtgaagatgg agatgtctgc
     8701 cagttgaact gaaacctctg ccattaaacg gcgcttctgg cggtcgcgta agccggcgga
     8761 accgaacaca gcgatcacgc gttttgtggt catctcgcgc gctgtttcca gcgccacctt
     8821 gagcgcgttc ggcgtgtgag caaaatccac aatcgcgcta taaggctgcc ccatctcaat
     8881 ggcttccatt ctcccaggca caccctgaag ccttgacaat ccctttatgg ccgcacctgt
     8941 ttcaactcct gccgcgcgcg cggccgcaaa cgctgccagg caattggaga cgttgaactt
     9001 accaggaagg ttgcagtgca cacgttcaac cagcccttca gcaatgaggg tgaaatccac
     9061 acctgatggg ttgctggtaa catcctgggc gaaataatcc gtcttatgct gcagaccata
     9121 agtgacctga cgacctttta ccaatccagc aagatagtca taagaaatat catcacggtt
     9181 cagaatggcc aggcgcgggt tacccaaagg tttttcaacg gtctgcgcca ggctggcaaa
     9241 catacgtcct ttggcagctc tgtattcctc gtacgtaccg tggtaatcca gatgctcatg
     9301 cgtaatattg gtaacaaccg cgatatcata ctcgcaagca gtcacccggt gctgggcaag
     9361 gccgtgagat gtggtttcca gaacaacagt tgtcagccca gccgccagca tcctcgacag
     9421 gtaaccttgc acttccaccg cttctggtgt ggtcacatgg aagccggtgt ccaccacctc
     9481 gtcaccaatc accgcgttca ccgttgagat aagcccagcc ttaacaccgg cttgctttaa
     9541 gatttcataa atcatattag cggttgtcgt tttgccatct gtgcctgtta ccccaataac
     9601 ggtcaactgg cgcgcggggt ggtcaaaaaa cgctgctgcc aggtacgcca gggcttcgcg
     9661 ggaatccgct acacggatgt aaggtacagg caggcctctg atttctctgg tgcccaccac
     9721 agccaccgct ccccttgaaa tggccgaaga aatgtactca tgcccatcca cgctgccgcc
     9781 gcttatcgcc acgaacaacg cccctcttct caccattcga gaatcataat aaatggcaga
     9841 gatgggaatc gcgccaggcg ctacgtctgt tgcccatggg atcgggaatt ccttaaaaag
     9901 atcggagagt aaaaccggtt tcatcaattt tccattctaa cattaaataa aaagagccct
     9961 ccttggagtg gcgttcagga agactctcac gactttctgc ttactcgagc gtggcgcgca
    10021 ggctctgaat ctggccggcg actgaaccgt cgatcacagt atcgcctaca cgcacaacca
    10081 ggccgcccaa taattttgga tccacattga aactgactgc cgcggtttcg cccagctttg
    10141 aaagcatctc cccctgtatg actttctgct catcccgcgt taaaggcagc gcgctcacca
    10201 cttcagcgtt tttgcctttc aattcgttct cttcaaggat ggtgaccttc ccgcccttga
    10261 taccggagaa gaattccgcc accagctcat gctggcgttt ctcgtccagg gcttcgccga
    10321 ccaatttctg tgccgcggcg atggccaggg cagcaacatt ggtgcgtatt tcaccaagca
    10381 cgcgttcacg ttcgcgttct acctcaaccg ctgcttcgcg cctggtcttg gcaatatcag
    10441 cctccacact ggctttcacc tcccgcccgg caagctccgc tttttctttg gcctcccgga
    10501 tcagctgagc cgcgttgttt tgcgcttctg ccagaatacc cgccgcgtcg cgttcagcgt
    10561 tttctcgggc ttcggcagca acccgcgcat cttccagtcc tttttcaatg gtttcacggc
    10621 gtttcttcaa tgaattctgt aacggggtat acacccacgc ccgtaaaacg accaggataa
    10681 caagaaagtt aattatctga acgatcagga acccgagatt cagacctagt ttttccaaag
    10741 caacctcctc attgaaagca acgggttata ccacaaagag catcagaaag gcgattacca
    10801 ggcaataaat cgcaatcgct tccgaaaaag caattccaag aatcatactg gataacaggt
    10861 tgccataagt atcaggattg cgcgccatgc cctgcaccgc tccttgaacg ctcaacccca
    10921 cgccaatgcc tccgccaagc gcgccaatca tagcaatgcc ggctccaatc atttttgctg
    10981 cttctacaat atcgccattc atgtcttttc ttgcctcctg gttgattact taccttcttg
    11041 aataaattga aaatttagtg ctctgcttca gcattatgcg tgatggtagc ctgcgccata
    11101 aagaccatgg tgagcatccc aaatacaaac gcctgtataa caccgacgaa caactcaaac
    11161 agcataatga acgacggcat gaacacgggc agcatcacac ccacaacagc caacaacacc
    11221 ataccggcga acatatttcc aaacaacctg aatgtgaatg aaagaatctt ggcaaattct
    11281 gaaatcagct caagcaaacc aacgataaaa tcgatgacgc caaacaacgg tttcttaaag
    11341 atcgtgcgca cattgatgaa tttttcgaaa tagcgcgctc cattcgcctg cacaccaatc
    11401 acctgaatca taaatacagc gatcagcgcc aaagcggtgg tgaagttcag gtcagttgaa
    11461 accccgcgca agaatggagc aagcacatag ccatctgccg ccggggtgtt tttcaaagta
    11521 aaccagccgc cccccaatgc ctgagtggcg aacccgtgct cgctgggatg aagaaaacca
    11581 atggtttcaa agccgggcaa aattttcaac aagttggcaa acaacaccac aatcagaata
    11641 gtggcaaacc aggggaaaat cttcgccgca tacttaccgg cggtattttc tgtgaggttg
    11701 taaagcatct ccactagcat ctcgaagaca ctaccaaccc cgcctggcgc gagttcattt
    11761 ttcttcgccg cgcggcgcac cccaagcgct acaagaatga tgacgatatc catcaacaac
    11821 atcgccgtga gcgtgttcgt caggtaaaaa tctcccacca caggcagggt gaacagaggt
    11881 ttttcgctga ggttttcggc tgccacctga atatgcggct gcaccggagc gatgaggtta
    11941 accccaatta cgctcaggat gattaacagg agcaggatcc agcgatgctt tccccaacgc
    12001 catttacgtt tattttccaa gccgcccctc ctcattgagg tgatcattgt ggttcgcctt
    12061 tattttggcg accgttttgc gcgtgatgta gaccatgagc agcagagaga ccgggatgct
    12121 cgccagcagc aaaccgatag taaaccaggg gcgggtgccc aatcgcgcat ccagagccag
    12181 ccctcccacc accgctgcca aaataatgat taaagttaag aagccgacct ggccagcgag
    12241 cgctgccagc caaaggttgc gccgatttgt cttccttgtt tcgttgtctc tttccgtgtc
    12301 aaacatgatt ttcgattata tcatagaggt ttttttgcta ttgataaacc gcctggaaag
    12361 gccaacttta tctaaaaccc tgtcaccgcg agggatatgc cattgagcca tggagcatag
    12421 ataacaccca ggagaataat acaggcaata caaagcgcga gcgccaccct ccactgtaaa
    12481 gctggagtca ccttccagct ggtctcatct ggtttatcca gatacatcac cttcattatc
    12541 ttcaaatagt agtacaaggc gatgacagaa tttagaatac cgagcaccgc caaccacacg
    12601 taatgcgatt gcaccgccgc gccaaaaacc agcaccttgg cgaagaaacc gccaaacggt
    12661 ggaatacctc cgagagagag gagcgcgacc atcatcacca aagccatccc cggagaacgg
    12721 cggtttaaac ccgcaaacgc gctgactgcg gaggagcccg atcccttttc aacccaactg
    12781 atgatggcaa aaaccgccag gttggtcacc aggtaaacaa gcaggtaata aacagtcccg
    12841 ctgaaaccga attcagtgcc ggcggccacc cccaccaaca tgtaccctgc ctgggcgata
    12901 gatgagtaag caatcaggcg cttgatatcc cgctgcgaga tggccaacaa attgccaagg
    12961 aacatactcg ccgcggcgag gatggcgaca atgaccatcc acgcattcga agaacccaca
    13021 ccaaaagcat tcaccagtac gcgaatgaag gcgccaaaac caaccgcttt tgaaacggtg
    13081 gagaggaacc cggcaactgg cgtcggagag ccttcatata catccggcgc ccaaaaatga
    13141 aaaggcgcgg cgctcatctt aaaagcgaaa ccagccagta ccagcagcga cgctagggtg
    13201 atccctgcga tcggcgctcc gttctgtttt atcagttcca acatgccatc aagacgggtc
    13261 gttccagcga agccgtaaag cagtgtgaaa ccgtataaca ggatagcaga ggaaacgccg
    13321 ccgaacagaa aatacttgat cccagattca actgattttt catcccgctg tttaaacccg
    13381 gccagcacat acagcgggat ggtagctgtt tcaattgcca ggtacaacat aatcaggttg
    13441 ccggctgaaa ccatcagcga aaaaccgata gaagaaagaa ccagcaagag gtaaaactcg
    13501 gcttttttcg cgatggtttg ttccatgcca gcaaaaatag cggtcatcgc gccgccagcg
    13561 aggatcaaca accggaagat gaaaccaacc cggtcgaagc ggatcatttc accccaaagc
    13621 agttgaacct ctgcaggggg gcgtgaataa aacaccgtca ggaagagggt gagcccaaac
    13681 ccaaacgccg tgacccagct gtaagccccg cccaggtttt cttttttaaa caacgagagt
    13741 gccaataaga acaaggccag cagagccagg atgagttcag gtaataatga gaggagcatt
    13801 tcagcagtga tcgccggcat tacgcacctc ctgtgagcac cagtatgtgc ctgacgccgg
    13861 cttcgatcca gggcaccaac acggacggga acaacccgat gacaataagg atcagcgata
    13921 acaggaccac aacgatctta tccataagcg aaatatcact tagcccagct ctgatgccct
    13981 ccggcgtttc accaaaaaag acccgcctga caacgagcag gatataggcc gcggtgatga
    14041 caatggaaag tgccgaaatg atggccacaa tcggggcggt ttgccaggcg cccatgtaga
    14101 tgggcatctc agcgataaac ccggagaacc ccggcatccc catggaaacc aggccaccaa
    14161 tgacaaagcc gagcatggca atcggcatgg tacggaacag cccgcccagt tcacggatat
    14221 cgcgggtgtg ggtacggtca taaaccatac ccacaacagc aaagaagaga gcggtcatga
    14281 tcccatgtga gaacatttgc agcccggcgc cggtgaggcc ttctcgcgta agggtggcaa
    14341 aaccaatgaa caccagcccc atgtgagata ctgaggagaa accaataacg tatttaaagt
    14401 ctttctgtat catggcgatg aacgcgccat acaccacgtt aattgtcgcc aaaaccagga
    14461 tcagcggcgc ccaataacgc ataccttccg gcagcaacat taacccggcg cgcaacgcgg
    14521 caaaaacgcc cactttcatt tccacgcctg ccagcaacat tgaaacagcc gtgggggcag
    14581 ccacgtgacc atcaggcgcc caactgtgaa acggaaacag accgcccaat acaccaaacc
    14641 ccacaaaaat gaccaggaac caggcgcgtt gaaattccag cgccaacccc gcctgttcaa
    14701 gtgccagcat atcaaaacta cgcagcccag aagtgaagta aaccgccagt acaccgatca
    14761 aagccaccac cgaacccagg aaaaggtaca gggtgagctt catgccgcca tactcgcgcg
    14821 tcttcgggga gccccacatc acaatcatca ggtatttcgg aaataccgcc aattcaaaaa
    14881 agaagaacag ctggaacagg tcaagcgaag cgaacacgcc aaaaacgctt gccgccagga
    14941 acaacaggaa agcaaagaac tcgcgcggtc ggtcctccac ccgccacgat accatcacgc
    15001 cgctaaacac aacaatacca gccatcagct ccatggcagc accgatgcca tcaaccccca
    15061 tgtggtaaga gatgcctaac gcgggcagcc agttcatctt ctcgataaac tggtaacctc
    15121 cggcggtggt atcgaaacgg aagaaaatga tgacagaaac cgccagcgta aacagcgaag
    15181 tagcaaacgc gatcccgcgt attagactgc gcttttcagc cggcagtagc agcacaataa
    15241 taccggcagc aagcggggtc agggcgagaa tactgagaag cgggactggc atctatctcc
    15301 tattaaaacc aggtcatcac ggtttgattg atcagattta aaatccaggc gggccaccaa
    15361 ccaatgatca caaccaggag catcagcggt atgatcacca ccagttcgcg ccggctgatc
    15421 tcagtcagtt tgccaaccca tttttcgttc aacgccccat gcagggtcaa tttgagcgcc
    15481 ttgagcacgt acacagcggt gaatagcagg ccgatcatcg ccagtgcgat aaacagggtg
    15541 aagataggcc acacaccgcg tacgatcata aattctgata caaacccatt caaccccggc
    15601 aaacccagag aagccatcga gataaaaaca agaatcgctc catatacagg gaggatggca
    15661 aagagcccgc cgtattcggc cagctggcgc gtatgagcac ggtcatacag cacgcccacc
    15721 aaaaagaaca taccggcagc cgtgagcccg tggttaaaca tctgtaatac cgctccgtta
    15781 agcgcaatca ccgcgctctc gcgcaaggtg ggattcgtct gtccacctgc ccaggccgct
    15841 gcggcgatac ccagtaccac aaaccccatg tggttgatcg aagaataagc caccaaccgt
    15901 ttgaaatctg tttgcgcggc agccgccagt gcgccaaata tgatggctgc cgtggccaga
    15961 gccgccagcc agctggcaaa acgcgctgat tcagccggga aaagtggcag caccagccgg
    16021 atgaacccat aaccgccaag ttttaaaagc acgcctgcca gcaccatcga gcctcccgtg
    16081 ggggcctcgg tgtgcgcatc gggcagccag gtatgaaacg gccaaactgg caccttgatg
    16141 gcgaaggcaa ccacgaacgc ccaaaaggca atggccttta ctgtagcgat gggaattcca
    16201 aacagctcac ccccttgcag cgccggccag ctggcagtga tcgttaccag gtcaaaggtt
    16261 ccacagacca ctccaatcat ctgaatggct aagagcagcc caagcgaacc acccatggta
    16321 aagaggatga acttaagcga agcgtaattg cgtttttctc cgccccattg attgatcaag
    16381 aagtacatcg gcaccaggcc aatctcccaa aagaggaaga acagcaccag gtccagcgcc
    16441 atgaacaccc caagcattcc cgtttcgagg accaggaata aaatcataaa cgccttgacg
    16501 cgttccttga tattaaagga tgtcatgatg gccagcgggg tcaataaagt ggtaagcagc
    16561 agcatcacca atgaaacgcc atcaatcccc agatggtagc ttgagccgat ggacgtgaac
    16621 cagggatagt tttctacaaa tttgaactcg ccaaccgtga gcgccgtgct gtacgacccc
    16681 cagaggacga gcgtcagggc gagagggatg aggctggcca ccagcgcaac acctctgatc
    16741 gctttaactt tgttggccgg gaacgccagc agaaccactg cccccaccag gggcaggaaa
    16801 aggatgagtg aaaggagatg cgctgagagg atgttcatcc agaccctgct tgcacctttg
    16861 ccaggtaaga taaaatgatc agcgccgcca ccaccgtgca ggcaacggca atcgccaggt
    16921 attgctgcac acgcccggtt tgtagcctgc gtaaaaagtc acccagcccg cgggttccac
    16981 ctgccaggtt gtcgcccgcg ccattgacca caggcagatc aaacttcgag cgcagcaagt
    17041 tccccagcca aacgccagcc gctgctaaac cgtgcaggaa accatcgatg agacgcagat
    17101 caatgaactg gtaaatcact ctatccgcca gccacaacac cgggttgatc accaccttct
    17161 ggtaaagctc gtctatccag tatttgtgct ccagcagcct aaagacacct ccaaaccgct
    17221 tttgcagcgg gtctggctcc tgcgcagatt cgtatccgcg ataaaccagc caacccagca
    17281 gtaacccgcc caacgaaacc aggatcgaga cgaccagggg aatgttgctg tgggcttcac
    17341 taacacttcc tgggctcaac atcgaaccca caaaagattg gaaccaggcc ggcgagattg
    17401 cccccaatcc tgggaaagcg tgcggaatgc caacccaacc gaaggtcaca gcgaacaccg
    17461 ccaataccac caacggagct gtcatcaccg gttttgtctc atgcgcataa gcggcggcat
    17521 cagatcttgg tttgctgagg aacaccagtg tgatctgccg tgcagtgtag aatgccgtca
    17581 acagcgcggt gacagccaag acgatgaaca aggcgagatg tccaccacca aatgcgccgc
    17641 tgagaatttc atccttcgac cagaacccgg cggtgatgag cgggaaacct gagagcgcca
    17701 gcccgcccgc taagaatgtc caaaaggtta ctggcatctt tttacgcaaa ccacccatgt
    17761 ggcgcatatc ctgcgcgtca atgtgtttgc ccgtgtgcag cgcgccatgt tccattccgt
    17821 gaataacaga accagacccc aggaaaagca gcgccttgaa gaatgcgtgt gtcaccaggt
    17881 gaaacacggc tgcggtgaaa gcgccaatac ccagcgcagc aatcatataa cccagttgag
    17941 aaatggtgga atacgccagt acgcgcttgc tatcattttg cgccagcgcg atggtggagg
    18001 tgaaaagcgc ggtaaatccg ccgatcaacg ccatcgccat catgggcacg gtcaaagaag
    18061 cgccatggtg ccatcctgcc gacatcaacg ggaacatacg cgcgctcaga taaactccgg
    18121 ctgaaaccat cgtcgcggca tgaatcatag ctgagaccgg cgttggccct tccatcgcgt
    18181 caggtaacca cacatgcagc ggaaactgag cggatttgcc gatcgtgcca cagaaaagca
    18241 gcaagccgat caatcccgcc cagttgagcc ccagcaccgg tgagagcgtg cttgtgagca
    18301 gggtcatcgt ctcggggtta gagaatattt catgaaagtt caatgtcccg gtcactgagt
    18361 acagcgccac catccccagc aacataaaca catcacctat gcgtgtggtg atgaaagctt
    18421 taatgccagc tttgcgcgcc gactccttgc cgtaccagaa accaataagg aagtaggaac
    18481 aaaagcccat gatttcccag ccgatgtaca gcgtcagcag gttatcgctc accaccagca
    18541 ggtacatggc aaaagcaaac aggcttaata gcgcgaaaaa ccgcgcgtac atcggctcaa
    18601 cggagggcac ccggtggatt ttgcccgctt ccgttatctc agccccctgc ggcgggagac
    18661 ctggacggtc ctgttttccg ataggctggc caaagttatg gtaaccgatg ctgtagatga
    18721 aaatcatcag cacagtccag gcaacaaaaa agagcataac tgcggttaaa ggatctacga
    18781 gcaccccaat cctcagccag ctcgcacccg tcggcagcca gttgatagat gccgcaaagg
    18841 aagaatccgc cacacctcca gccgtgaccg ctttaatgaa aaccaccatg ctcccaacaa
    18901 atgaaaggcc tgccccagcc aacgccacgc tgtgactggc tcgatgagac cgcctggtaa
    18961 aaagcacgat caataaaaac ccaagcaagg gcggcagcgg gatcagccag atgagcgggt
    19021 gaatattgaa catgcgaaaa attgtttaac ctttcaatag gtcaagttcg ttggcaacga
    19081 ctgtgcgtcg tctcctgtag gcagaaacga taagccctaa acccacagcc acctcagccg
    19141 ctgcaaccac aaagacaatc agcgcgaaga tcaagccatc catgtctcca cgcggtgcgt
    19201 gcaggtagcg ccaaaaagac aaaagattga tgttcgccgc gttcagcatc aattcaatac
    19261 ccattaaaat ggcgatggta ttacggcggg tgatgacagc aaaaagccct aaagagaaaa
    19321 gaaacgccga tagcacgagg aaccagtaaa gcgggatcac ctctcctcct ctctacggct
    19381 aatgcccaca tgcacggcgc cgatcaaagc cgccagcagc agaatagaag aaacctcgaa
    19441 gggaataagg taaccttgcg ggtcaagcag cgacaacccc atttctccaa catcccgttg
    19501 cgcatctgcc agcggagcgg tgaaaagagc ggattgcggc caggtgaaaa tagccagcag
    19561 caagccagaa agcgcgactg caccaattag ggctgtcaaa acccagcgat gattttgaag
    19621 aaaagagtca tccacagcat cacgtgtgag catgatggca aaaaccagta aaatggcgat
    19681 ggcgccaata tacaccacca cctgaacaat ggcgaaaaag ctggcttcca gaagaccgaa
    19741 gaccaccgcc acgccaaaca aagtgagaat caaccacatc gctgcgtgca tcaagcgctt
    19801 cacagtaacc atcatgacgc ctgcaatgac ggtgacggcg gcattgatga gaaagacaat
    19861 ctgcatcccc gtcatgattc tacctctaca ggctctgcgg gtttttcgcg aagggtaaca
    19921 gctataggtc gcggcccaga taccacccgc cgttcctggt tttttcgcct gtggtaaatc
    19981 gaaacgagtc tgtccgccac cacgaagatt accaggttcg ccaatagcag acctcccagg
    20041 cgcaaccaac cctgcccggc aggtagggat ttatccacca gtgctgtcac cagcaatgag
    20101 accagcgcca gcggggtcag gtacttccag ttaaacgcca tcacctggtc aactcgcaaa
    20161 cgcggcagcg aaaagcggat ggagatggtg atgaaataca cgatggccgt tttaatgagc
    20221 aggtaaagga atccgagtgc cgggtacaat tcagcgccag gtccgcgcca gccacccagg
    20281 aagacgctgg caaaaatcga cgccgcggtg aaggcgtgca gaaaatccgc cacgtaaaac
    20341 atgccgaact tcaagcctga gtattcgata ttaaagcctg ccacgatctc tgaatctgct
    20401 tcgatcaggt caaaaggtgc gcggctgttt tcggcaatgg atgcgatgaa gaaaaccagc
    20461 gccgccactg gcgccttcca gatgaaccac actctttgcg cagaggtgat gtcgttcaac
    20521 cccatcgagc cggttaacat gactggcacg agcaaggcga gcgccatcgg gacttcataa
    20581 ctgatgagca tcgccaccac tcgaaagccg cctaaaagcg cgtacttatt attggaagca
    20641 aacccggcga gaatgatgcc cagctcacca agggcgccca ccgccaggat ataaagcacg
    20701 ccgatttgga tgttagaacc gtataccgtg gaggaaagtg gaatgaccgc ccacaccgcc
    20761 agcaccgcgg ccatggccag caccggcgcc aggttaaaca acaccgggtc agacccccag
    20821 ggggtgatat attccttggt gaaaatcttc agcatatcag cgatggtctg gaagattccc
    20881 caagggccaa cgcggttggg tccgatgcgg tcctggaaac gtccgccaag tttacgttcc
    20941 agccagatga ggaagatggt gaacaccatc gcccccatac tgagagcgcc agcgcccagg
    21001 atgaaaagca tgaactgcgc ccacccagga ggaactcccc aattactcag gacggaattc
    21061 agccagttac tgatgaaagt gatggggtcc gcgaaaaaac tcatccgagg tctcccgacg
    21121 gctgccttac gtccattcca gcgctccttt gcgccaggcg taaaccaggg cggctgccag
    21181 gatggctaca aatagcacgg cttccagtac ggcaaaaagc gtcaggcggt tataggcaac
    21241 ggcaaaagga tataagagca cagtttcaac atcgaaaatc aagaaaagca acccgtaaag
    21301 gtaatactga gctttaaatt gaatgcggct gggacctacc gtttcaatgc cgcattcata
    21361 gatcgagttc ttgaggggcg aaggtttttt tggagacagc aacgcggctg ccccaatggc
    21421 tgccgccgga atgaacatgg atatcgtcag aaagatgacc agatacaacc actcgtgcag
    21481 catagtgctc ccagtaaacc ttgtgattca atgcaggcaa attttatcat atttgccctt
    21541 cgctgcaacc aatctcaata ctgctttaat cggcagcgga gggtctgccc ataccccacc
    21601 accagcagcc cgctcgccgc caggaatccc gcagaaatcc tgaccggtct gaccgccagt
    21661 cccataaacc cacgcgattg gagagtaaac tcccgcgcca gcaggagttc atccgccgcg
    21721 tcagaagtaa cccatatcca taaggttgcc gggtacacag cctcttcacc tgttcgtaac
    21781 ttccaaatga gccccacctc ggagccgctt cgcaagtgca gtgtcacccg tccagctgga
    21841 gccaattcct caaatcccgc ttccagcctg gccgcgagct caaggttcat gggttcgctg
    21901 gaagaactgt tgactttcac tttggcgttc agccggaccg catcgccaat gaatacctgg
    21961 cgcggcaggc caactatgac cgtcccggag cgtcctgaaa ccgttgtaaa ggaaacaacg
    22021 gcggtatcgc gtaccttgta aaaagtaaaa gcgaaaagaa aaatcgagca acaaaggagg
    22081 gttaaacccg cgtttcgcca tgcagtcttc ataaaaagat tataaccggc aaaaaaatac
    22141 cggcaaatca cgccggtatc ctcaacgaaa actgcttact cacctggtaa tgcagatgaa
    22201 aacatggtct ccatcctgct gatcatccac ttccacctga aacggctcgt tctgtttaag
    22261 cgcctgcccg atctcaagca gcatttcctc cacgcccttt tctttcaccg cgtcaggcat
    22321 acattgccca aagttgctga atacccatga gaccaggctt aaaggcaagg ggatgttaag
    22381 aatgatgttt tgaagtctgc ccttctcatg tgttcttatg cgcacctgca gccagggaga
    22441 tgtcatcaag atccagccca acaccataat caacactccg atggctaagg gtatccagga
    22501 aagccagaaa ctccagccca accctgcttt ctgatagcca aggtaaatcc agtcagcgga
    22561 aaacgcagtc aaaaccgccc ctcccaaaag gatcatgctc caggcggcct tccagcagcc
    22621 tgagactcgc agcgtttcct gatcggctcc aggggtctct ggttccagcg gcggtcggat
    22681 gaattccctt tcgctttctt cagcaccagc ggcatctaaa atgcctatca gttcagaacc
    22741 ttcttccggt gttaaggtcc cgtcctccac cttgcgcaaa atttccagtt ttgattcatc
    22801 cgtagtcata acgaattttc cttatttttc cagcgctgaa aggatttttt cagcctcttc
    22861 aagcgtgatc tttttctctt gtaacatctt caataccata acgcgttcct catcagtagc
    22921 cttgctggtg ggagttgggg tagaagcatt ttcgcctgga aaggtgaaac ccaccacctt
    22981 gtcgttttct ccacggcgaa accctgtggc gttgatatcg ccacacacac cgggtttttc
    23041 aggcagatct atcgtgattc cgcgttcact gagccttttt cctctctcat ccagtttgcg
    23101 catggcttgc tccagtttta ggcgaacttt ttcaccagcc gccgccgtgc ccagggttgc
    23161 ccatttgatt ctttctgagg tggctgccac gctctcgcga acccgctttt ccaggtctga
    23221 accaaagcct tcccaattct caaacatctc atcccatctg gactgaaaaa cgccatccag
    23281 gtcatcatga tcaatgatca agatgtcccc ttcggcttta atactgacta cattgccgcc
    23341 ttcgccaaac gggatgacta cctgttcctg atcccattct ccctgctgac cgcaggcatt
    23401 caggcggatg gaacctcctt ctgtgtaaag ttctagttga gcattacacg ccgcgtgtac
    23461 ccgtattttc acgtctccgc cggctgttat cgtcaccatt gctggattgg ctgatgcaag
    23521 ttgcagcact acatctccac ctgccaccag gttcaggctg cccttcacat cccgcagata
    23581 aatatccccg cccactgcgc ttgaaagtac tgattccacc gcactgccaa caagatcacc
    23641 gccaacccgc gcaatctcta cagcgcctgt tgttcgttgc aacgaaatat ccccccccac
    23701 catttcaacc gaggcgccag ccagatcaac aagcgccagg tcacctccca ccttgccaat
    23761 gatcaccctc tggatcaaac cgctcacatg ggcgtctccg ccggcctttt ccacagttac
    23821 cggctgcaag gcaggcagct gcaaggatag gtcgctggtc gctcgaatct ccaccttgcc
    23881 ctgatcttca gagaatttga gtgaatcgct ttcctttacc aaagccgtca tgagtagctg
    23941 gttcttgccg gaaagtgata gatcatctga aacgattatg ttcacctggc tatccgtttt
    24001 aatcggcatg gatacttgtt tcatacgccc tcccaatcaa ttctcattca tttatttatg
    24061 agtatatcaa ttaattttag tattgtcaat actattatta atattctttt gtttatacgc
    24121 tctaatacct taattatatt tatttctatt ttcacttatt atgcaactga attgctcaaa
    24181 ccattatcga tcagcgcgcg gcaataggtg tatactcact agcgccaata tggaccagga
    24241 aacgaggaaa caatgaaaaa catcaccgta tctaccgcaa tagtgctgct cctttgcttt
    24301 cttttaatta ccgggtgcgc tcaaaaacag gctgtcactt atttatcggc agatgaaagc
    24361 gctcaggtgg caacctacac cgatgttgcc gcccagaaca tcattaatgc catcgccgcg
    24421 aacgattaca acctgttcat cagcgatttc gatgaacaaa tgcgccatgc gctgcccgaa
    24481 gagcaatttg ccaccattgt gaaaatgtat gtcaaaaacg gtcaggcaga gagcatttct
    24541 ttattaaaca tggaagacag aggagatttc atcggggcca attatggtgt cacctactcg
    24601 aacgctgcgc tcaccatgtt gatcgtcgtc gccaaatcag atccttcgct ggtaagcgga
    24661 ttatggttca aatagatgtg aaatgaaaat gaccgaaatg ttgtttataa tatcagcatg
    24721 gtaattcttt acgctggatg aataaaaagg agtataccta ctatgaaact aaccccccct
    24781 aaaaatataa cctgggtcat cgccctggtc gttggtgttc tcggtatcat tggtcatctg
    24841 gcgggtttct ttggcgactt cgctttttgg ctggttgtga tcggctttgt aatcctcgtc
    24901 ctctctacac tgctcaaaga cttgtaagtt tgccgtgccc cggggaaacc cgggattttc
    24961 aattcagtga agagaattac ctcaaaaaaa tccggctgtc gccggatttt tctattcaat
    25021 ccgcttgaaa atgtaataat catccgcttc agccagcgaa agcaaactcg ccaggaagtg
    25081 gctgctggtg tagtcttccg gttggtaaga gaaccaggcg accaggcggt aattatccct
    25141 taaccattcc agctcttctc cattcaaggt atagccctgg tacgttttat cgtgtttaat
    25201 atgctctcgc tgcaccaacc agtcagggcg gtaatccatc acaaattctg gccaccagcg
    25261 ctcctcataa gccaggcggt aattcaccac ttgcggtgac accaacccga cctcatcata
    25321 ggtatataac cctgagtaat aagggatgta accggcaggc tctaacaaca tcttatcacc
    25381 tgcctgggcg tgttctttca gccagacgcc aattcccccc cgataagcga attctttggt
    25441 tccccaaaca tacgagaaca ccatttgcgc atttagccct gccaacatca gcagcacgca
    25501 tccccaacgt accacctgcc tccacttccc agtccctgtt gaccactcca cgatcaacgc
    25561 cagtaaaaat accgaaccga aaatggcagg cggccaggtg taccactggt agagcacccc
    25621 tccgaaggcg taagccaccg gcaccagcca ggtgatactg gcgagcgcca aaccggtgat
    25681 acgcggcgct ctatcactcc atactcgccc gaggtaaagc cccatcgcca gcaaaacgag
    25741 caatccaaac agaatgcctg ccctgccgaa aatttcagcc ctgtaaggag aaaacgcgcc
    25801 accggcgttg ccgacgaaaa cggtgaacag gttgtccgcg aaacggctta tcgtgaacgg
    25861 atgccgcatc agcagcttgg cattgatgga ctgctgcagc aacgtgccaa aataaaggga
    25921 attaaagata agcagactgg acacaccgat taccactgcg gtcaatccag cccaaagcac
    25981 gcgctttcgc ttgacgcaat cccaaaagag aatcagtaaa gcgtaagcaa tggcgtcggg
    26041 gcgcacccac ggnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    26101 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nngccaggaa
    26161 cacgtaaagg tgtgaagtgg aagcgctgac ccgttcgccc gggttgaaac cataaacacc
    26221 gctctgcgcc aggttttcag cgcagcgcag actgatatac gcgtcttcct gtatgtgatg
    26281 tgtgaaatag aaaggcactc gcgcagcgag gagcaagagg aaaagcagcg cgaaacgggc
    26341 agcgggttgt tgaagaattc gccgtgtcaa tcggagtccc tgtcttttct catctggaaa
    26401 agcttagccc cggttaccag cagcaagggg atgagcagcg cgcctccaca aaatggggtc
    26461 cctggcctgc ggttcaggtc tttttcgggg ctttcagctg cggcagtttg taacgccgcg
    26521 gctgaagcag ccaccggcgg agacgaggtt ttcgttatca atggctgaag ggtgggggtt
    26581 acgccatcca gtaggggctc aaactgaacc gcggtcagca cgggggtttg ggtgagccag
    26641 ggaaagtcaa acctctcatc cacctccaca atgccctcct ccatcagcca ggtgaggtct
    26701 actgcgatgc ctttcactgt ttcgtcatca ggcgccaact gtaaagccag gtgaatttgc
    26761 tccagtgctt taagcttcaa aggcagcgtc tgctcacctt cgtacccggc gtacaccgcg
    26821 tagtacgcca gcaggtcagc gtagccggcg tgccataacg cgtcctgcgg taacaggtct
    26881 gtcgcttttg tgtacgcttc atcgctcaac tcgaatagct cttttacacc ggggtctgcc
    26941 tgaaaatgcc aggtcctgaa accacgcctg ccatgcggcg aaaagagcag cgttttgtaa
    27001 agcttgccca acctgcccca ggcctcgcca tctttcgggg cggcggccac ccgcgcgcgt
    27061 tctctcaata cctgtttcca cacagaaggg gcgaccaggg agatggtgaa attgtcttcc
    27121 ggctttggtt caaagtcacg gtactgccag ctgagcgttt cgccctcttt tacccagccg
    27181 ggggtggtgc agttggttgc atcatcgatg cagggcagca aaaagagctc atccacctgg
    27241 taaggcaggt tcacaatcag gtccgcgctg ccaatggtgc ctttccagcc agcgcctgta
    27301 gagaagatat actggaacca aatatagggc atctcgccgc tggcttcaag ggtgtaagcc
    27361 acctcgatct tcactggttg cccggcggga aacaacacgt caaattccac ccagtccacc
    27421 gctttttcaa agccgttggg gtcttccccg ctgatccgac gcgtgttcga aggcgtgccg
    27481 ttgaccttga ccgagatgtc ttgaacaggt gttacgccaa accagccatt gcctaccgcc
    27541 gccgggaagc gcaccgccat gcgctcatcc gtgctgctca ggtttttcat attgaacagc
    27601 gcctgtacat ccgcgaagcc catttcctcc ccgccgtcag cctcccgtat attcaaccgc
    27661 acgctctccg actccatgcg cacctgtgtt tgctcactgc ccggagcggg gttgaaccct
    27721 ggggggtagg ttggaggagc aatatccgcg cgcgctggcg cgatggcgga caatataaac
    27781 aataaggcga taaccagggc catcttgcgc atgctatgct ccttgaacag atattttcga
    27841 tggatgaggt tattgatatc tgataatttt cttctattct caatataaaa caaaaataac
    27901 ctgttaaatc aactcaaaag tattttcacc tgaattgtat tcgattagtt ttacccaatt
    27961 aatggctccg ttttcttgac aaaagtacct gttgaactcc atcgaaaatc gattaatcat
    28021 atttacatat aattctgaat actcttcatt acgtttcttc gcctcaaatc ctaaaggctc
    28081 aattaaatca gtatataaat tctgttcccc tgaaataaaa ctccaaaact tctggccgca
    28141 aaatttatag tactccccct tgtctggttt gttatccctg ccgtaacaac aaccgtttac
    28201 agcggtaatc gataatttgg agttactcgt tcttaaagtc ctcttggctc ttgaaaaatc
    28261 atctatcagt tttgatatct gagatgagtt cccccaatta ggacctgact taatattgac
    28321 aatgtatctt atcccttcat aatcgaactc gaggtcaatc ccaggaatgc cggacttcca
    28381 tccattgaat acctgagaat taataaaaat tgccaacccc tccaaccagg tgccgaaaat
    28441 tgtctcttca tttgaggaaa tgtgcgagtc cacaaatcct cggacgatca tctccgaggt
    28501 cagcatgttt tttgctttat ataaataggg gtttttgcgc tgtaaaactt ttgttaactt
    28561 aagggcgcta aggctattaa ttcgatcttg gtgaaattct ccgatatgtt catgaacata
    28621 agatttcaca tcctcaagtg aaagattatt catcttgttt tctttccttt tttatgagtt
    28681 ctgctttcaa gcggttaata gccacttcac aatattcatt attaatttct atcccagtgt
    28741 acatacggtc aagaagagca gctgccacgc aggtagtgcc tgaaccattg aatgggtcca
    28801 gtacaaggtc accgggtttt gtaaaaagcc tgataaacca ttctggtaat gctactggaa
    28861 aaacggctga atgctgctga ttgtagcatt cagtagccat gtaaagaacg ttgctcggat
    28921 aaaccaagtc ccttccaacc cagttttcaa ttttcttacc aaaaccactg ccaaccctcg
    28981 aattgtccct ttttttatca gttgttgaaa gatttttcag tctggtttga gcccaatctc
    29041 ccacgggcac cattacttcc tcttgataca tagcaaattt tttttgcttc gtaaagtgca
    29101 atagtctctc ccagttgtcg cgaaacctgt taggccattt tccagggtga gaatttttct
    29161 tgtgccacat gtattcttca gtccaatacc atccatgtct tcttaattca aggattaatt
    29221 caatcacata cgtgtgccgc tcgccattaa ccacccgttc tttgatattt aagatgaagg
    29281 atccttcagg tttcagaatc ctgtaaaatt ccttggcttt tggtagaaac caatttacat
    29341 atttttctgg tttaattccg ccgtatgtac tagtcctctg gtcagcataa ggaggagaag
    29401 taaaaatcaa atcaactgaa ttatcaggta atcctttgag gataagttca caatctccat
    29461 taataataga atttttataa ccctcaacaa ccagaggcga atcaactgcg ggctcaataa
    29521 acaatgggat ttgttgcgaa ttaatgatat ataacccgcc agaatcctga aggttcggcg
    29581 aaacaccttt attcaatcga tatactacac aagagtcatc catatccatc aacaatgatt
    29641 aaaccatatt tttcacaagc ctgaacaagc gagtgatcga ttaagacaga acaggcaata
    29701 aggtctgatt taattgcgcg gaacagacgg atttgcatcc tacctcaatg acaaaccgcc
    29761 agcctctaaa caggttggcg gtagttggta gtgttggtcg gggcgggcgg atttgtgtca
    29821 tacctcgatg gtaaaccacg acctcttgtt ggtcggggcg ggcggatttg tgtcatacct
    29881 cgatggtaaa ccacgacctc tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    29941 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    30001 ntgtcatacc tcgatggtaa accacgacct cttgttggtc ggggcgggcg gatttgaacc
    30061 gccgacctct cggtcccgaa ccgagtgctc tagccggact gagccacgcc ccgaacgcct
    30121 gcattatacc ctaacccgga aaaatcagag agggttcgcg cttccggctt ttattcgtta
    30181 caattaacct gttgtgaaac gaccttccct gcccttgctg ctgttcatct gctggctgct
    30241 tgccgcctgc gccgcgcccg caacgcccgc cgtcctgccg ccgacgttta ccatccagac
    30301 ggctgccatc gcctccactt cactgcccag cccgaccagc gcgcccacct ccaccgccac
    30361 gcccgcaacg cccgctcccc ccgctgtaac tctgccccag ggcgcgctca gcgcgcagca
    30421 gcgccaggcg ctggaaaacg ccgctcgcgc tttcatcagc ccctctcccg aggaagcgct
    30481 cgctaccgct attgagctcg gttacctggg gcgcaacgcc aatcccgcca ccatgtgcgg
    30541 cccgttggcg ttggcgattt tgcagcaatc cggtcttgtc gattcttctt tcccgttgaa
    30601 gagcttttac tacctcaatc cacgccccgg tcatgacgaa cgctacctgg aaaaggtctt
    30661 tccagcggat cgatttgaga agatcgtgcg cgaagaatcc attggcaaaa tcgacttcag
    30721 cgcagaccca cttttgccgg gtgatttcct ctatctgttt gccggcgaca gcggcagttt
    30781 tgagcacatg ctggtggtct cgcgcgtaga tgatgcgggg cgggcgtacg ctgtgaccaa
    30841 cctcaacact gccagggggg tgattgtgga tgaggtgctg ttgtatgatc ccgccaaccc
    30901 cggcgttgga cagttttacc agtggacaga ttgggagaac cgcatgatcg gacgaaccgg
    30961 ttatggcggt tactggctct ggcgtccgct gccctgattt tcccccttca cccccccaca
    31021 cacacacttg ttgccgcttt ctattcatgg ataaaattca ggcctgaata ttgcaaccag
    31081 atacaagata tgaccagttg cgtgttttgc gatatcatct ccggcagcgc cccttcagac
    31141 cagttatacg cggatgaaga gctgctcgtc atccaggata tccacccggt tgcgccggtg
    31201 catgtcctgg tcatcccgcg caagcacatc ccctcgctca accatctcca accggaagac
    31261 caggcgctgg cgtcccgtct gctgctcacc gtgccgcgca tcgcccatca attgctggga
    31321 gatgacgccg cttaccgtac gatcatcaat accggcgctg ccgcggggca aacggtcttc
    31381 cacctgcatg tgcacatcat ctccggccgt cctttcatta atcatctgct cacccggggg
    31441 ttgtattgat gcatcaatcc gcccgcctgt tgttcgcgat actcgctacg ctgctcatca
    31501 gcggctgcat caaaccagcg ccggggtcag tgccctggca aatttcgggc acgcaggaac
    31561 ttccgggtgc gcagcatccc gtgacctcct ccccaccttc ctggatgcct gctacccgcg
    31621 ttcccggatc gccttatttc acacccacgc cagatacgcc gcgggttatg cccaccttgc
    31681 gcgctgacca ggagttttac acggtgcagt atggcgacag cctggggaac atcgccctcc
    31741 tttacaacct cgatctggcg tcgctcatcg cggcaaacga cctggcgaac ccggatatcc
    31801 tggaaattgg tcaggtcatc accatccccg caccgcaacc cggggcggaa ccttcatcct
    31861 ttaagataat cccggattcc gagctggttt acggtccggt cagttccacg ctggacattg
    31921 aagcgttcgt gaacgaacgg aatggctacc ttaacgccta ccgcgaagaa gtggaaaaaa
    31981 tagaaatgag cggggcacaa attgtggcgc gggtaagccg ggagttttct atcaaccccc
    32041 gcctgctgct ggcgctgctg gaataccgtt ctggttgggt gactacttcc ttaatcgatg
    32101 attcgttgcg cgattacccg atggggctgc aagacccggc gcgcaagggg ctttacctgc
    32161 agctggcatg ggcggccaaa ctactcaacc gcggttatta cctttggaag atcaacgcga
    32221 tatctttcac cagttttgct aaccacagcc tggcgctgct ttcaccgacc atcaacgctg
    32281 gtacagcagc ggtgcaatac atgctggggg ttggctcgta tcctgatgcc tggcaaagag
    32341 cggtctcaga agaagggtta ttccgcacct actgcgaatt attcggcatc ccatttgacc
    32401 tggctttcga gcctttgatc cccgctgacc ttcaacagcc agcgctcacg ctgccctttg
    32461 aacccggtac cctgtggtct ttcaccagtg gccctcacgc cggttgggca gacggctcag
    32521 cctgggccgc cttagacttc gcccctccgg gtgagcagct cggctgctac atcacagacg
    32581 aatgggtaac cgcggttgct gacgggctca tcgttcgttc cagtagcggc gcggtggtgc
    32641 tggacctgga tggtgacggc ctggagcaga ccggttggac catcctctat atgcacatcg
    32701 aaacccgcga ccgcgtcgcg gcaggcgcac gggtaaaaac gggggaccgt attggccacc
    32761 cttcgtgcga agggggtttc tccaatggcg cacatgttca cattgccagg cgctacaacg
    32821 gcgaatggat ttccgctgat agagaaatcc cattcaatat gagtggctgg gtctcttcgg
    32881 gtgatggcag tgaatacagc gggacgttga ccaaaaacgg taaggtggtt tattcctgga
    32941 acggacgggt agatgcgaac caaatcggtt actaggctct ttcagatagt cgccgcctgg
    33001 gcggtattag ccggctcaat gctagcttgc tcgcagggtt acgtgagccc ggcggagctt
    33061 acagctaccg ctggcctccg ccccatggca gacacaaccg cggcgcctac agaagaaacc
    33121 ccttttaccg ccaacatctc gctgtttcaa acgccaaccc aaccacccct gcccacgttc
    33181 actgacctgc cggcgtttac cagcacaccg caaccctcac gcacgccaga tcctaacgct
    33241 acgcccaagc cgcccctcca atattacact caggcgggtg atacactggc ttctatcacc
    33301 ggtcgttttg gcgtcagcgc cgaccagata attcccagcg aacccatccc ggtggatggg
    33361 ttgatcaacc ccggggtgct gctcatcatc cccgacgtgc tggaagatgt gtatgaatcg
    33421 ctgaccttgc tgcctgacag tgaagtggtt tattctcctt ctgcggtgaa tttcgatatt
    33481 ctgaagtacg ttgcagaagc ggatggttac ctcaaccgtt accaggagac agttggttca
    33541 cggcagtaca gcggcgcgga gctggtacag cgcgtagcac tggaaaattc gatcaacccc
    33601 taccttttgt tgaccttact ggaatacaaa agtcactggg tcagcagccg acccactaat
    33661 atggcagaaa gcgaataccc catgggatac gtgaggttgg aataccgcgg tttgtacaag
    33721 caactctcct gggcggtaga gcagctttcc atcgggtatt acggctggcg ggcgggcagc
    33781 ctgaactcgc ttacttttaa agatggttcc agcgtgcgca tcagcccggg tttaaacgcc
    33841 ggcaccgccg ccattcatta cctgttctct cgctggtaca accaggctga gtgggcagcc
    33901 gcgctctatg gcgctgataa catgcccaat ctgatgagcc acatgtttgg tgacctgtgg
    33961 gaacgctcac gcgcggttga ccccctttat ccagcagacc tacagcagcc tgagttcagc
    34021 ctgcccttcc agcccggccg cgtgtggagc ttcactggcg gaccgcactc cgcctggggt
    34081 gaaggtggcg cgctggcggc gcttgatttc gccccgccat caagcaccgc gggctgcgtg
    34141 gcctcaaatg agtgggtgac agcggctgcc tccggcgtca tcgtgcgtgc ggcggatggg
    34201 attgtgattc aggacctgga tggagacggc gtcgagcaga ccgggtggaa tatcatgtat
    34261 ctgcatgtcg aaacacgtga ccgcgttcag cccggtacat acgttcaaac tggtgacaaa
    34321 atcgggcatc ccagttgcga aggcggcata tccaccggaa cccatgtgca cgtggcgcgc
    34381 aaatttaatg gagagtggat cctggcggac ggtccgctgc cctttacgtt aagcggttac
    34441 gtggcgaaaa acggcggctc cccttaccag ggcagtcttg aaagcgggca ggcaaggatc
    34501 atcgctaatc cattaggaac acttcgatct tacatctccc ttccctagcg caatatcctt
    34561 ccatctggta taaaatttac cggtatgaaa tcaacaggaa gtcgcataaa taaccaccgc
    34621 ggcggtttcg gttcgctgtt cagcgcgatc atgagtatcc tgatcctgtc tgcctgttcg
    34681 ccgcaacctc ccataacagt gtacattact ccgtttccgt taagcgccac cgaagccccg
    34741 gctcaacagc ccaccgcgct ggccacgcgc ccggtttatc aacccggcac gctggtggcg
    34801 tataccgcgc gcacaggtga caacctgcca gcgctggcgc accatttcaa cacaacggtg
    34861 aaggaaatcc ttgaaaacaa ccctcaactc tctgaaaaaa cgaccacgct cactcccggc
    34921 gagaacctgc agattccgat ttattacgaa gcgctttggg ggaacccctt ccagataatt
    34981 cctgactctc tctacatcaa tggtccagcc caaattggct ttgataccaa gtcattcgtc
    35041 aacgctcatc ccggctggtt gaagaactac gcctcgctcg ccggcggcga agtgcgccgt
    35101 ggcgctgacc tgattgatta cgtctcgctc gaatattcca tcagcccgcg cctgctattg
    35161 gccttggccg agtaccaggc aggagcgcta agcgtagaaa cccttgaacc gcaagaaaaa
    35221 gtttatccgc tcggctatga agatgaattc caccagggtt tttaccttca acttctgtgg
    35281 gcagccaaca ctttaaataa cggctactac agctggcgct ccggcaagct gggcaccatc
    35341 accaggctgg atggctccat tgaacaccct gacccctggc agaacgccgc gacggtggca
    35401 ctgcagaatt acttctcaca ggtgcttccc gttgccgatt atcacaaagc catttacagc
    35461 gaagggttat acaatacata cgctgcgctg tttggcgacc cctggcaaca cacctctgac
    35521 cacattcccg gtgatttaca gcagccagcg ctgaggttgc cattcgtgcc tggaaagacc
    35581 tggtcttaca ccggcggacc gcactccgcc tggggtgaag gcgcaccatt ggctgccctt
    35641 gactttgccc ctccatctgt cgtcggcggc tgcgccgcca gcaacgagtt tgtcaccgct
    35701 atggcggatg gtcagatcgt gcgctctgaa aaaggcctgg ctctgctgga cctcgacggc
    35761 gatggtgatg agcataccgg ctgggtgatc ctttacctcc acctcgggct gaatgataag
    35821 attcgccccg gtatgctggt gaaaacaggc gacctcatcg ggcacccttc atgcgaaggc
    35881 ggttccgcca ccggcacaca tgtgcatatc gcccg
//
LOCUS       DAOTLW010000017        35760 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1425_length_35760_cov_9.640022, whole genome shotgun sequence.
ACCESSION   DAOTLW010000017 DAOTLW010000000
VERSION     DAOTLW010000017.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35760)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35760)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35760
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1425_length_35760_cov_9.640022"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            327..926
                     /locus_tag="P5516_04205"
     CDS             327..926
                     /locus_tag="P5516_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRT91673.1"
                     /translation="METNKKKSGCGCGLAMLILLVVVVLTSPLILRGIGALLIYADPL
                     KEADSVVALSGDTGDRVAEAARLYQKQYASYLFITYTDEPARDALIRAAVIEGIPADR
                     VIVTEMQVSNTVDEARAIKALAKERAQDSLIIITDPFHTLRTRIIFRNVFRGSGIDVQ
                     VRPVGGHWYRSTTWWRTAEGRRYTLEEYLKILLYFFGRY"
     gene            966..1607
                     /gene="udk"
                     /locus_tag="P5516_04210"
     CDS             966..1607
                     /gene="udk"
                     /locus_tag="P5516_04210"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HRT91674.1"
                     /translation="MTKHKKPLVIGIAGGSGSGKTTAADIILKKVGSHRIAYLQHDSY
                     YRELTNLPINQRREVNFDHPDSLESDLMREHIMALKEWKAIEVPVYDFTTHSRTSQTL
                     HVEPRRVILVEGILIFAEPALRSLFDVKIFVDTDADIRFIRRLKRDLEERGRTTEMVI
                     KQYLTTVRPMHLDFVEPSKRYADVIIPEGGLNEVAMDMVIARIESMLRDDSNP"
     gene            1604..2146
                     /locus_tag="P5516_04215"
     CDS             1604..2146
                     /locus_tag="P5516_04215"
                     /inference="COORDINATES: protein motif:HMM:NF020893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VTT domain-containing protein"
                     /protein_id="HRT91675.1"
                     /translation="MSKLSGWKLTFVRVLVLLAVITLTIFLILNRDRVQELEALGYPG
                     IFLISLFSNATLILPVPGVLFTSAMGAVFNPYWVALAAGCGAALGELTGYVAGFSGQG
                     IIENKQWYARVTEWMKKFGGLTVLLLAFIPNPIFDIAGMVAGALRMPLWKFLLFSWVG
                     KTGKMLVFALGGAGLLELLT"
     gene            2263..4989
                     /locus_tag="P5516_04220"
     CDS             2263..4989
                     /locus_tag="P5516_04220"
                     /inference="COORDINATES: protein motif:HMM:NF014758.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRT91676.1"
                     /translation="MIKKSIMVNGIPRIIIADPEATLAEVLRVQLGLIGTKVGCNEGH
                     CGSCSVIMDGKLTRSCIVRMNKVPDWACITTVEGIGTPKDMHPIQKALVFHGAAQCGF
                     CMPGFVVSSKALLDVNPSPTRDDVRAWFQKNLNACRCTGYKPIVDAVMDAAAVMRGEQ
                     PMASLEFKMPEDGRIWGKSYPRPSAVAKVTGTADYGADLGLKLPTSTLHLALVQAKVS
                     HANILEIDTTEAEKMPGVFKVVTYKDIKGKNRINGLVTWETNRCDGWDRPILCDQKVF
                     QYGDAIAIVCADTKAQALAAAEKVRVELEELPAYMNAPEAMADDAIEIHPGTPNVYFT
                     QGVVKGEDTKPLMASAAYTAEGSFYTQRQPHLTIEPDMGFAYYDDEGRLTIHSKSIAL
                     YLHALMIADGLGVDASNIRMVQNTAGGTFGYKFCPTIEALVGVACIATQRPVYLEYDY
                     EQFITYTGKRSPFFTWLKLGADKDGRLIAMETDWIVDHGAYSEFGDLLTHKGAQFIGA
                     GYDIKNIRGRGRTVCTNHAWGAPFRGYGAPESEFPSESLMDELSEKIGMDPLEFRYLN
                     VYRPGATTPTGQVPEVFTLPELIDLIRPKYQEAKKRAAAESTAEKKKGVGVAIGIYGA
                     GGDGPDTAEAWIERTPYGVLVSTTWSDHGQGADAGALGTAYEALRPLGLRPEQIRLEL
                     NDTGKAPDGGAAGGSRSQVVTGNAIKVCCENFLAAIRKPDGPYLSYDEMKEDDMPMRY
                     SGVWTSPTEIGDPDTGQGNPIANYMYGVFLAEVVVDIKTGKVTVEKMTLAADIGKINN
                     KAVVDGQLYGGIAQGIGFALSEDFEDIHKHTNMVKCGIPFAKDVPDEIELIYLETPRP
                     SGPFGASGVGELPNTAPHAAILNAVYAACGARIRELPAYPAKVLAALQQTSQ"
     gene            5072..7381
                     /locus_tag="P5516_04225"
     CDS             5072..7381
                     /locus_tag="P5516_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT91677.1"
                     /translation="MDQRRLHELEEQCIQDCDPPCTSRCPVHVDVRQMCKEISAGDFK
                     HALITFQKSVPFPEIIARTCDQPCQALCNREPLGGAIRMADLELACCQLGMEESLLSP
                     VTGRKNALVSVIGGGLCGMTAAWELARKGYRVVLFDQAEQLGGQLWGLTDAQLPKEVI
                     LRESAKLKNKGIDLRLKTRVDLNAFDWAQYDAVLIATGVDQESVNWLEKNRQGNTVID
                     PVTFESGMSGVFCGGSLLHPPSTITSVADGKRTATSIDRYLQKVSLTASRSSEGTQDT
                     RLYTNLAGVLPVFAVPPADALQGYTMEEAIAEASRCLVCECMECVKVCTYLSELGAYP
                     RKYVRDIYNNLSIIMRQRRGNKFINSCTLCGLCGEVCPTDLNFAEVIVQARKTMVETK
                     KMPISAHDFALRDMNFSNGPRFALTYPAPGEGKCDYLLFPGCQLAASNPEYISLIYDD
                     LVRLVGNLGIMLRCCGAPAQWSGEETLFAESGAVIRSVWESLGKPKMILACSSCHQSL
                     KQLIPGLETVSLWEMLLKNEALGTTRGGCTQEYKVHDPCTSRYEPDWQHYARAVIERL
                     GVRYSELPMSRTLTECCGYGGVAWLANPDLVHKMIQRRINEDNANYITYCVMCRDLFV
                     SEGKPTLHLLDFIYGRDISALAIRKPPDYSQRHENRIRVKQRMEKRLLVKGDWKMETY
                     EKINLQLSSEMRDLLEARLILVEDLQKVIEHAEASGEFFFNKNNGHSIASFRPNLITY
                     WVEYEKQENSYTVFDAYSHRMVVGGDTSI"
     gene            7587..8351
                     /locus_tag="P5516_04230"
     CDS             7587..8351
                     /locus_tag="P5516_04230"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT91678.1"
                     /translation="MIADCATCGLFSFQPNPGICVHTHRPGGLVITERALALCDLPER
                     ARLLDVACGAGATVNFLSSKAGLKVTGLDRSGKALSGIQGKPILLNLVRSDLLNIPMA
                     PEYFDGVFMECALSLTGEPLNSLLEIRRVLKPGGKIVLTDIYLREVMTETIQRVLAGS
                     ACLAGAMTQDGVEEVIAAAGLTRLVWEDHSPLLKQWLVEHIFRLGSVSGLFQQLTREN
                     EQNERAAQALSRVRLGYYLAILEKPLSFKSQEAYDG"
     gene            8341..8781
                     /locus_tag="P5516_04235"
     CDS             8341..8781
                     /locus_tag="P5516_04235"
                     /inference="COORDINATES: protein motif:HMM:NF021252.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C-GCAxxG-C-C family protein"
                     /protein_id="HRT91679.1"
                     /translation="MMDDLSKIRRFGEQGFYCSQVILLMGLEWQGKEDPALVRSMHSL
                     AGGIGFSGHNCGALTGGACLLGYFAGRGSTSEEEDDRLFLMLNELVDWFTRKYGVEYG
                     GIDCENILSGGKDQYVARCPGIVQSTLQKCKELLVEYGYELNGE"
     gene            8756..10144
                     /locus_tag="P5516_04240"
     CDS             8756..10144
                     /locus_tag="P5516_04240"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT91680.1"
                     /translation="MAMSSMVSEIPVEGCVICRTQSICPVCLNRLEAKRVEYEDGVYL
                     EKHCREHGFFRTILWRGRPDYGSWRREKLPNHPRHPFTQVERGCPYDCGLCAEHRQEP
                     CCVLLEVTSRCDLDCPVCFASSGEKDATDVPLSTISLWYERMLKAGGPFNIQLSGGEP
                     CVRDDLAQIIAMGKKAGFSYFQLNTNGLRIARERDYLASLKEAGLGVVYLQFDGTTDD
                     IYRTIRGREILDAKKKVILNCRALELGVVLVPTVVPGVNDKDLGNIVRFALENYPVVR
                     GIHFQPISYFGRFPDQLRNEDRITIPEILRNLQEQTEGLVKTEDFHPKGSENSYCSFH
                     CTYILMPGGELLAVKNQAGKTCGCREPEDSSKALLRTRSFVARNWIYPTPSAPCSCGE
                     SASMGEWDVILERSKTHLFSVSGMAFQDAWNLDLDLLQDCCINVAADDGKLIPFCAYN
                     LTNTLGEGLYRL"
     gene            complement(10469..10762)
                     /locus_tag="P5516_04245"
     CDS             complement(10469..10762)
                     /locus_tag="P5516_04245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91681.1"
                     /translation="MVKTGHPVQMNWGTNQYVHFFLPNDLQYTPFIINRAIFGGQAAQ
                     FTQGSEKQVSYAARPLARQQDQHSVPCTCQRGHARSKKVYGQRLVFLREKNPF"
     gene            complement(11476..11880)
                     /gene="rnpB"
                     /locus_tag="P5516_04250"
     ncRNA           complement(11476..11880)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="P5516_04250"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            12013..12771
                     /gene="tpiA"
                     /locus_tag="P5516_04255"
     CDS             12013..12771
                     /gene="tpiA"
                     /locus_tag="P5516_04255"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HRT91682.1"
                     /translation="MRKPLVAGNWKMNKTVEQASLLARELLTGLQQVPEVERVLCPPF
                     TSLMILSAQLSGTGVGIGAQNMHWAESGAYTGEISPQMVREFCEYVIIGHSERRTYFG
                     ETDESVNRKLKSALALGLVPIVCIGETLEEYEAGVTGEVITRQVLEGLKDIGTASASI
                     IVVAYEPVWAIGTGRAATAEGANAVLRDHIRPALAQLFGSGLAQNLRILYGGSVTVAN
                     ATEFFRQPEIDGALVGGASLKVDEFVKIVEAARP"
     gene            complement(12823..13449)
                     /gene="upp"
                     /locus_tag="P5516_04260"
     CDS             complement(12823..13449)
                     /gene="upp"
                     /locus_tag="P5516_04260"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HRT91683.1"
                     /translation="MSNLHVSAHPLVAHKLAKLRDARTEPKKFRELVREIAGLLAYEA
                     TADLVMEDIEVTTPLATASGKILKDKIGLIPILRAGLGMVEGFWDLMPGAEVWHIGIY
                     RDENTLKPVEYYNKLPTEPTVSVCLILDPMLATGGSATATVEVLKRWGVKRIKFVGII
                     GAPEGIAAMTKAHPEVPIHLAAVDERLNNVGYILPGLGDAGDREFGTA"
     gene            13548..14585
                     /gene="ruvB"
                     /locus_tag="P5516_04265"
     CDS             13548..14585
                     /gene="ruvB"
                     /locus_tag="P5516_04265"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003059357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HRT91684.1"
                     /translation="MGFMTADERIVNPVPKVDDRVDNVLRPSKLKELIGQDQVRQNLE
                     ILIAAAKGRKECLDHVLFYGPPGLGKTTLAYILANEMGVNIKVTSGPAIERTGDLAAI
                     LTNLREGDILFVDEIHRLGRSIEEVLYPAMEDFSLDIIIGKGPSARSLRLKLPRFTTI
                     GATTRLALVTAPLRARFGAVYRLDYYDLTAMRKIVERAAGLLNVTSDDTGIEEIARRA
                     RGTPRVALRLLRRVRDFTQVRANGLITRETAINALDLLAVDAMGLDDVDRRVLKTIIE
                     KYNGGPVGLATIAASISEEADTIMDVVEPYLLQLGYIDRTPQGRVATPLAYEHLQLPY
                     NQKDNQPRLIP"
     gene            14588..15607
                     /gene="queA"
                     /locus_tag="P5516_04270"
     CDS             14588..15607
                     /gene="queA"
                     /locus_tag="P5516_04270"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HRT91685.1"
                     /translation="MQTADFDYHLPPEYIAQTPIEPRHASRLLVLRRSSATLEDSHFC
                     QLPDFLNPGDLLVINQTKVIPARLHARKTSGGKVELLLLKQREPGLWEALAGGKGLRK
                     GVRVQLENGPYVDIEEELTGSERLIRFSEPVEQLLPTIGEMPLPPYIHEKLLRPDRYQ
                     TVYATKTGSAAAPTAGLHFTPWLMDEIERKGVRIAKVTLHVGLDTFAPVTELDPVEHK
                     IHTEWCELTEEAALLINRTKASGGRVVAVGTTSVRTLESAAATSNQPSWVAPYRGQTG
                     LFILPGFEFKVVDALVTNFHLPKSTLLMLVSAFAGRERVLDAYQAAMERGYRFYSFGD
                     AMLIL"
     gene            15616..16341
                     /locus_tag="P5516_04275"
     CDS             15616..16341
                     /locus_tag="P5516_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HRT91686.1"
                     /translation="MTCPRSLKLLDNQIIACRTCPRLVAWREKIGQEKRRAYLGQTYW
                     AKPLPGFGDPLARVLVVGLAPGAHGGNRTGRMFTGDASGDFLFSALHRAGFANQPEAK
                     ALNDGLMLNDVYITAVCRCVPPDNKPLPAEISNCLPYLQYEFELLEKVEGVVVLGRIA
                     FDTVSKLLYHQRKLATSAIFGHGVLQPSTDGAPWLLCSYHPSRHNTQTGRLTTTMFDQ
                     IWEQVNRLLGKDELLSCQEPGSS"
     gene            complement(16309..17067)
                     /locus_tag="P5516_04280"
     CDS             complement(16309..17067)
                     /locus_tag="P5516_04280"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT91687.1"
                     /translation="MKLYDSFASVYASGDYPNLSQRMAAILPGVMKHYKILTNNRKKL
                     LDVACGEGSFCVEMAKNGWSVTGVDQSEEMLRLAKHRAKNDQVDVKFEKQDMRFIDYH
                     NEFDLATCWFDSLNYLISNNDLQSTFNSIARSLKPNGWFLFDMNTIYGLAVEWQKNRC
                     YVRQETKDLLELHRTCYDFEKNLGCIKITWFVKVDDKWEKFEEKHIERGFSIAEIESC
                     LEYAGLHVVDKVSSLDPISPLGSQSGRAWFLTRK"
     gene            complement(17220..18506)
                     /gene="eno"
                     /locus_tag="P5516_04285"
     CDS             complement(17220..18506)
                     /gene="eno"
                     /locus_tag="P5516_04285"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HRT91688.1"
                     /translation="MEIASVEALEILDSRGNPTVEVRVILEDGSIGTAAVPSGASTGI
                     HEALEMRDGDKNRYNGKGVLQAVEHVNGEIAENLIWWDAIDQKAIDQMLIELDGTPNK
                     SRLGANAILGVSLAVAKAAAASLDLPLYRYIGGVYAHVLPVPMMNILNGGAHTGWQST
                     DAQEFMVMPLGAPSFAEGLRCGDEIYQALKSVLKSRGYPTLVGDEGGYAPALKANSEA
                     VEVILEAIEKAGYKAGEEVAIALDPAASEFYEEETKTYNLRKEGKKLSGEQMVEFWLN
                     WVRQYPIVSIEDGLAQDDWDSWKLLTKEIGHKVQIVGDDLLVTNTSRVRRAIKEEACN
                     ALLVKVNQIGSLTETIEAVEACHRAGWRAVTSHRSGETEDSTIADLAVALNTGQIKTG
                     APARSDRVAKYNQLLRIEAELGETAAYAGWSVLKAY"
     gene            complement(18594..19988)
                     /locus_tag="P5516_04290"
     CDS             complement(18594..19988)
                     /locus_tag="P5516_04290"
                     /inference="COORDINATES: protein motif:HMM:NF016357.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HRT91689.1"
                     /translation="MPKPRLLIPVFTHFSVRYILRTGMLTALTEFTQPIVALSWQDPA
                     LENEFSQAGAEILHVPNFTFSPQFKETRELVNFWHKQVRQTNTTEIDIRREASLLGLR
                     ERVNYEVQILKDRIKTALPARIPHLLQQEESLFWSETNYQEFDSILDQSKPDAVFSIT
                     PFVNKEEPLLRCAQKRGVLRITSILSFDNVTSRTRLPVSFEHYLVWNRYNHAELLRAY
                     PDITPEQITISGPPQFDFYYDSANVIPEEKWRSRLGLPSARPVILFGGGTYKIVPNEP
                     LFLKQLDDAISNGEVPAHPIILFRRHPGDIASRWQEVLSGVKNTFTDQSWQANESRGQ
                     INITHADIEQLCSTLAHCAVQINSSSTLTVDGAVFDRPQIGPAYDPGSSRKYDRVLKE
                     LYIREHYIPITNSGGLDLVYSRPDFIRAVNDGFEKPAERTEGRKKIVEEICTYNDGQS
                     TLRVVKKLRQLLGG"
     gene            complement(20067..21710)
                     /locus_tag="P5516_04295"
     CDS             complement(20067..21710)
                     /locus_tag="P5516_04295"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003883 - CTP synthase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006241 - CTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HRT91690.1"
                     /translation="MTTKYIFVTGGVVSSVGKGVTAAAIGLILKERGYKVAVQKLDPY
                     INVDPGTMSPYQHGEVYVLDDGAETDLDLGHYERFIDIRLNRVCNITTGQVYAEVIAK
                     ERRGDYLGGTIQVIPHITNEIKRRINLVSKSTSAEIVIVEVGGTVGDIESLPFLEALR
                     QLRSDLGRENTVNVHVTWLPYIGATDEIKTKPTQHSVAELRSIGIAPDMIVARSDHPV
                     AENLREKIAQFCDVEKRAVIPMVTSPILYEIPLLLEKEKVGEYLLHRLNLDSKQQPDM
                     SDWEKLVSETAREKPRVSVALVGKYVELHDAYMSVREALKHAALSLGLELDLRWVHSA
                     DLEKAKHVNALEDAHGIIVPGGFGSRGIEGKIWAANYARVNLIPYFGLCLGMQLMCVE
                     FGRYALGSDQVNSAEFDLSTPHPVIDLMPDQQNIKDVGGTMRLGLYPCILQPGTIAAN
                     AYGVKKVEERHRHRFEFNNAYRERMAQHGMVFSGLSPDKSLVEIVELKDHPFMLGTQF
                     HPEFLSRPTRPHPLFLAFLRAAAVKAGVAYRDPPLLYKR"
     gene            complement(21900..22733)
                     /locus_tag="P5516_04300"
     CDS             complement(21900..22733)
                     /locus_tag="P5516_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRT91691.1"
                     /translation="MEMNAETQDNQQLEPKMPFKARVIKVSLAGAVLDIGIGKPALLH
                     VSQIIAPDNTQNKKVDELLKEGEEIDVWVKKLTRKDSEERIEMTMFKPLDLEWREIKV
                     GETVKGKVVRLEKFGAFVEIGAERPGLVHISEMAHGYVKLPSDVVQEGDEVEAQIIEV
                     NRRKKQIKLSMKAIQPEPEKVVEEKPAPAASGAEGARREKKSARKPRRKSEVESSGVL
                     EVKNETVEPEPTYMELAMRAAMEKAKQKKKKQEVHRTKPGSTEQDEILARTLENKVQT
                     N"
     gene            complement(22828..24096)
                     /locus_tag="P5516_04305"
     CDS             complement(22828..24096)
                     /locus_tag="P5516_04305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRT91692.1"
                     /translation="MNTNHTGRIILVIMISLVLLAGAFSGGVILGWLIPSGSPLETLA
                     PDTTKPAETPPLPDSTPPSSTPADLRKLFQPFWEAWDIIHEQYVDQPVDDLQLMRGAI
                     QGMLNSLGDIHTGYMTPEDYDQATTTLDGSYGGIGAWVDTAGEYLTIIRSMSGSPAEA
                     AGLKPGDEIIGVDGQSVIGKDPSAVLQTVKGPEGTTVVLTVQRHAPEEILDFTIVRAQ
                     ITVSSVESRMLDQGIGYLALNTFGSESAQEMETALAGLLAQHPVGLILDLRDNSGGYV
                     DAAIDIISQFIGSGVIMIERFGDGKEVTFEAHPGGLATEIPLVVLVNQYSASASEITA
                     GAIQDYQRGKIVGTTTYGKGSVQNWVPLADEQGAVKVTIARWLTPKGRLIHGIGLTPD
                     YIVERTENDIEAELDPQLEKAIDLLLNANK"
     gene            complement(24378..25031)
                     /locus_tag="P5516_04310"
     CDS             complement(24378..25031)
                     /locus_tag="P5516_04310"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HRT91693.1"
                     /translation="MSFAVMLFDLDDTLYPSTSGVWDAIGDRMDSYIIKTLGVAANEV
                     KQIRNGLFHEYGTTLRGLKTLYHIDECEFLDYVHDIPLTQFLRKDEALIETISAYDSR
                     KVIFTNANQRHAERVLEVLGLVDFFPEIVDVLQISPYCKPLPQAYQKAMEIIQIEDPE
                     HCVFIDDSPRNLKTAHEMGFYTIQVGTENRCEFADAAILSLLDLPDVIPSSHRTMER"
     gene            25106..26857
                     /gene="mutL"
                     /locus_tag="P5516_04315"
     CDS             25106..26857
                     /gene="mutL"
                     /locus_tag="P5516_04315"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HRT91694.1"
                     /translation="MSIQVLSEELASQIAAGEVVERPASVVKELLENAIDAGASRISI
                     RVEDAGKKKIEVSDDGTGIPIDELPLAVSRHATSKINTAEDLFHIRTLGFRGEALASI
                     GSVSRLMVLSRNADSQVGARIMVEGGRIVSKEMTGSVTGTIVRVSDLFYNTPARLKFL
                     KRDVTERQQIDTLVSRYAMAYAGKAIHLEHDGKAVLRTSGNGERREVLAQLYGIELAR
                     QLLEVVFEEEGMKIEGYISPVSISRSNRKDMTFFINGRWVQDVTLVSALLKAYQTYLM
                     VGRYPLSILFIEIPPEAVDVNVHPAKAEVRFREPDRVFTAVQRAARRALMAYVPIPPL
                     AAASWSSRGWNPSPELDGTWMNARPVQPQSTGQPQPMAETPLSSSVEPAPFAAGIPLL
                     RLVGQIGATYLVAEGPDGLYLIDQHAAHERVLYEKMQRQKENIPTQPLLEPLLIQLPP
                     QSAQIICDNLQVLRKIGFHLEEFGRNSFRLSAIPALFTQKDPRAAINSVVEDFEEDEE
                     PLKEVHEDRLIARICKRMAVKGGSILSTEEQQALLRDLESCSSPRTCPHGRPTMIHLS
                     VELLERQFGRRGARSAF"
     gene            complement(26854..27627)
                     /locus_tag="P5516_04320"
     CDS             complement(26854..27627)
                     /locus_tag="P5516_04320"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HRT91695.1"
                     /translation="MTTPVEHELRRAADLIASSKYFVAFTGAGISTPSGIPDFRSAGT
                     GLWQRDDPMKVASATAFRQSPRNFYNWLRPLLRVSYSAHANDAHLALATLEKAGKLQA
                     VITQNIDELHQKAGSVNVVELHGSIHRFYCPANKEHGVDFAAVVTSILSGEIPLCATC
                     GTVLRPDITLYEEELAADDWLKAENEIALADLLLVAGSSLEVTPAALLPFKACRNGCK
                     IILVNHSATSIDPYAELVMRSDVAECIPAMVALLNQRCG"
     gene            complement(27624..28778)
                     /locus_tag="P5516_04325"
     CDS             complement(27624..28778)
                     /locus_tag="P5516_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HRT91696.1"
                     /translation="MPEKILIVDDDMETLRLVGMMLQRQGYEIAAANTGELALETAVK
                     VKPSLIILDVVMPGMDGYQIARELRKNPLCTHIPILMFTAKNQMGDKIAGYGAGADDY
                     LTKPVHPAELNAHVKALLDRMKKIKNTPEESGSGCIIGVLGCKGGLGVSTLVLNLAVS
                     LSGMTSKEVVAVELKPGQGSWGVELDIANSYGLEKLLALPVDEITAAAVEKLAVHTSF
                     NVRLLMASEKSQKISLPDLPEKTLAIIKSLNSSIPVLVLDIGNPYLAGFSKVHKLCDQ
                     LIVLTDAFPATVLRTRKLLGTLQFEVPKKNRYIDTVFYNHIRSEAGLTAEQITKLLPG
                     TTIKFMIPPVPEQVFQANQKYIPLYHIQPEGLFSQQVNELSRAICQRFQL"
     gene            28912..30324
                     /gene="tilS"
                     /locus_tag="P5516_04330"
     CDS             28912..30324
                     /gene="tilS"
                     /locus_tag="P5516_04330"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HRT91697.1"
                     /translation="MNIQEFQAILQTRCAIDLKKPVLLGFSGGPDSICLLSLLVKSGV
                     EVTAAHLDHALRPDSAAEAVQAEEICRQLGADFISRRVDVADYAQKKHLSVEEAARKQ
                     RYAFLFAEAAKTGAQAVLTGHQADDQVETVLMHLLRGSALSGLAGMRMVLLPNPWSKD
                     IPLVRPLLAFSREEVEAYLSEIPIRPIFDQSNQDSSFFRNRIRNDLLPQLLTYNPQFK
                     KRILRMADAIALEDDLVMAQTRQAWAETVIETGEDFVVFDRAKTASLHPALRRRLMRE
                     GLTRLRSELRDMDYQVIEKGSHFFTDPCQSNTVHLIAGLEMFAHLKRRVVLAKKESPL
                     HGLWPQLTQKKALPLKIPGMVSLNEHWTLRSSRETIYINTGDPFVCQLNAELLADKLT
                     IDRFKAGDRFSPYGAAAQEMKLGDFWTNQGLAARARSNWPLIRSGEEIVWVPGFRIAD
                     RWRVKKETTSILRLELIKTE"
     gene            complement(30361..31251)
                     /locus_tag="P5516_04335"
     CDS             complement(30361..31251)
                     /locus_tag="P5516_04335"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HRT91698.1"
                     /translation="MDIQPELHITSETTFHPAIRSWEIFLLDQGRSLHTVKAFLGDMR
                     LFANYFPSGTTIGSITTDDINRFLGWLQHERHVPCSAKSLSRRITSTKSFFRWLSRSG
                     RIPSDPAEKVLQKTVISPLPDVLTPEEEVLALEAARALRVASKPDPRPYVLLSLLLST
                     GIKKGECLALTLNHIDLDSPKSPRLFVRYSASGSRYKERNIQLNEDWVEAYQEYLDVY
                     HPVEQVFPWSPRRLEYLLEDITIDAGLSKHISFAMCRWTCALNDWKSGMDKEALRQKL
                     GISKIQWRETSNKLRLLAGE"
     gene            complement(31336..32376)
                     /locus_tag="P5516_04340"
     CDS             complement(31336..32376)
                     /locus_tag="P5516_04340"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRT91699.1"
                     /translation="MRRLLLASLFVFFLLLSACSTFTTDLKGNTPIPISDLLTGEGGR
                     FTATPTPFQPVFQGINPPSVFTSTPSVTPPPGTGGMIALGLARPASQVNILLLGSDWR
                     PGQGYRTDVIMILSLIPDSNRVTLLSFPRDLYVNIPGIGYERLNSAQAYGGFPLTAET
                     LKTSFDASVDYYMMTDFSGFKNIIDTLGGIQVYATSDLYDRCDLPQAYAKMCYIPAGW
                     NTMNGETALWYARSRYSTSDFDRTRRAQEVAVAITQKMISLNALNRAGELYQLFLSSV
                     ETDIPLDLIARLLPMAATAADNPSSIHRFTITEDQTTNYIVPATGAMVLLPDYDSIAR
                     TIQDALYPSLEH"
     gene            32549..33124
                     /locus_tag="P5516_04345"
     CDS             32549..33124
                     /locus_tag="P5516_04345"
                     /inference="COORDINATES: protein motif:HMM:NF021886.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRT91700.1"
                     /translation="MTLKHAILGLLNYEPMTGYDLKKTIDISINHFWPAVQSQIYTTL
                     AKMEKEGWVTVETIPQESLPPRKVYSLTPTGRAEFVDWLTTPQPYGETRIAWLVQVFF
                     AGQLDDQKALLLLHNLKSVFRQRLEGYASIPEDNRDSLRNDPPRDKFFWMLTVDFGVA
                     QTISQMQWIDKVIRTVESHEYILPTLAGDRS"
     gene            33154..34653
                     /locus_tag="P5516_04350"
     CDS             33154..34653
                     /locus_tag="P5516_04350"
                     /inference="COORDINATES: protein motif:HMM:NF012993.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HRT91701.1"
                     /translation="MKKLLGAGNKPWHFIAGIFLSLLSAGMLLLAFHPYNQWYLAFFA
                     LIPMRLAEYRFFPRKWAGLGSGIGVGVWLAVFLGRMFGFSGPALVIEIVVLAIVLVQV
                     FTIPATRQFHERTAYRWFILEGIAGWVGSEMIRSFIPPINTHAFIAQTMYTQPWMLQP
                     ISIFSIYGLSTLIVLVNYTLLQGIILFLDKKQLFFNLPEMNRRSTSRWLTVAGATLIL
                     WLGISGAILLSRPANPTTVRVAAVQLGISAPAHQDTAESQSTRLDVLAELSRIAATEG
                     AQLIVWPELGLGFDPQVEHTGELISLAAETDAYLLIGYGLDDPRGWRNEMVMLSPQGE
                     FLAVYGKNHPTSPGEPRIITAGVYPVYDTPIGRLATIICNDVHFTNTSRRLARAGAQL
                     IAVPTLEISGITLEQVAQSVMRAVENRVAVVKADVAFSSAIIDPYGKIVALRNASPQG
                     AAFTLTADVPIGSGKTVYNWLNLDWMGWLNLVGMVFFSFGAKWLEKKVR"
     gene            34819..35619
                     /locus_tag="P5516_04355"
     CDS             34819..35619
                     /locus_tag="P5516_04355"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT91702.1"
                     /translation="MNHKVGDAGWIRSFYDSAVGWWGESWYEGENLKNRLDIVRKYAS
                     NIDKRILELAAGTGETAAYFCDHGYGVLAVDISHCNCELMINLQLGKSNLRVVEGDFL
                     KLDIKEQFPTVCMFESFGFGSDQEQQQLLKRVNENWLIPNGVLILDVYHPAGPIRACG
                     KKQELDKLENIAGSVDMTEYSYYDPIKSRWIDIWEPRDNKENRRVQSIRCYTPADFVL
                     LAAGCGLSIEKMLYRGMEFDHETPEVTTENIFDDDSNYSYTVILRKKE"
BASE COUNT         8566 a         8690 c         9661 g         8843 t
ORIGIN      
        1 aatcccttcg gtggctcaat catttccggc aaaaaaagct caggtcaagg gtttactggc
       61 aggaacatcc agcactcgca caaggcgctc gcaaatccct tcggtggctc aaccaattcc
      121 ggcaaagaac ctcaggtcaa gggtttactg gcaggaacat ccagcactcg catagggcgc
      181 ttgcaaatcc tttcggtggc tcaaccaatt ccggcaaaga agcccaggtc aagggtttac
      241 tggatgcggt tgacatgacg caatacactc tccaaggctg ttgtttttaa aaggtcactt
      301 tgataaggta aaatgaagaa atggacatgg agacaaataa gaaaaagtca ggctgtgggt
      361 gcggtctggc gatgctcatt ttgcttgttg tggtggtact gacctcccct ttgatactac
      421 gggggattgg cgccctgctt atttacgctg atccgctcaa agaggcggat agcgtggtag
      481 cactgagcgg cgataccggc gaccgggtgg cagaggcagc ccggttatat caaaaacaat
      541 atgccagtta tctcttcata acctatactg acgaaccagc gcgggatgcc ctgatacggg
      601 ctgcggtgat agaaggaatt ccggctgacc gcgttattgt gacggaaatg caggtatcca
      661 acacggtaga tgaagcacgc gccatcaaag cgctggcaaa ggaacgcgcg caggattcac
      721 tgatcatcat cactgatccc tttcatacgc tgcgaacgcg catcatcttc cgcaacgtat
      781 ttcgcggaag cgggatagat gttcaggtgc ggccggttgg cgggcattgg taccgttcca
      841 ccacctggtg gcgcactgct gagggtcggc gttacacatt ggaagaatac ctgaaaattt
      901 tgctgtactt ttttggcagg tattaaatta ggatgctttc cgatggattc taatataatt
      961 attccatgac caaacataaa aaaccacttg ttatcggaat cgccggtgga tcgggctcag
     1021 ggaaaaccac tgcagcggac atcatcctga agaaggttgg ctctcaccgc attgcgtatc
     1081 tgcagcacga ttcatactac cgggaactga ccaacctacc catcaaccag cgccgggaag
     1141 tgaactttga tcacccggat tcacttgagt ccgacttaat gcgggagcat atcatggcgc
     1201 tcaaagaatg gaaagccatt gaggtgccag tatatgactt taccacccac agccgaacca
     1261 gccagacact gcacgttgag cccaggaggg tgatccttgt agaaggaatc ttgatctttg
     1321 ccgagccggc tttacgctcg ctttttgatg tgaagatctt tgtagatact gacgcggata
     1381 ttcgctttat ccgccgtttg aagcgtgacc ttgaagagcg cgggcgcacc accgagatgg
     1441 taattaaaca gtacctcact acggtgcgac cgatgcacct ggattttgtg gagccctcaa
     1501 aacgctatgc ggatgtgatc atccctgagg ggggactgaa cgaagtggcg atggatatgg
     1561 tcattgcccg aattgaatct atgctgcgtg acgacagcaa cccatgagca aattaagcgg
     1621 ctggaaactt acttttgtca gggtgctggt gcttctggcg gttattacat taaccatatt
     1681 tctcatcctc aatcgtgaca gagtgcagga actggaagcg ttgggatacc cggggatatt
     1741 tctgatttcc ctgttttcca atgccacgct catccttccg gtgcctggcg tgctgttcac
     1801 ctcggcgatg ggcgcggttt ttaacccgta ctgggtggcg ctggcagccg ggtgcggagc
     1861 ggcgctcggc gaactgacgg gttatgtggc tgggtttagt ggacaaggca ttatagagaa
     1921 caagcaatgg tacgccaggg ttacggaatg gatgaaaaaa ttcgggggac tgactgtgtt
     1981 gttgctggct tttataccca acccgatttt tgatattgcc gggatggtgg cgggcgcgct
     2041 gcggatgccg ctgtggaagt ttctcctctt tagctgggtt ggaaagaccg gcaaaatgtt
     2101 ggtttttgcg ttgggtggag cggggttgct cgaactgttg acttgaaaac ttcccgttga
     2161 aaattcttac tatctcctat ttgattgtga atattaaaaa atccatatag aattatttca
     2221 atcgcgaata aaaaaaccac atgctatcgc caggaggaat tcatgatcaa gaagagtatt
     2281 atggtaaatg gcatcccgcg catcatcatt gctgaccctg aagccacact tgctgaggtt
     2341 ctgcgtgttc agctggggtt aatcggtacc aaggttgggt gtaatgaagg gcattgcggc
     2401 tcttgttcgg tgattatgga tggcaagctg acacgttcct gtatcgtcag gatgaacaag
     2461 gttcctgatt gggcctgcat taccacggtt gaaggaatcg gcactcctaa agatatgcat
     2521 ccaatccaga aagccttggt ctttcatggg gcggcgcagt gtgggttttg catgcccggt
     2581 tttgtggttt cgtccaaggc gctgcttgat gttaaccctt cgcctacccg tgatgatgtg
     2641 cgcgcctggt tccaaaagaa cctgaacgcg tgccgttgta caggctacaa gcccattgtg
     2701 gacgcggtga tggacgccgc ggctgtgatg cgcggcgaac aaccgatggc aagcctggag
     2761 tttaaaatgc cggaagatgg aaggatctgg gggaagtcct atccgcggcc ttcagctgtt
     2821 gccaaggtga ccggaacggc cgattacggc gccgaccttg gcttaaaatt accgacatcc
     2881 accttacacc tggcgctggt tcaggcgaaa gtgagccacg cgaatattct ggagatagat
     2941 actactgaag cggagaaaat gcccggtgtg ttcaaggtag tcacttataa agatatcaag
     3001 gggaaaaatc gtatcaacgg gttggtaacc tgggaaacca atcggtgtga cggctgggac
     3061 cgccccatcc tgtgcgacca gaaagtattt caatatggag acgcgatcgc tatcgtctgc
     3121 gcggatacca aagcgcaggc gctcgccgcg gcagaaaagg tcagggttga actggaagag
     3181 ctgcccgcgt atatgaacgc gcctgaagcc atggcggatg atgcgatcga gattcacccc
     3241 ggaacaccga atgtttattt tacgcaaggc gtggtgaaag gagaagacac caaaccgctt
     3301 atggcatccg cggcttacac agccgaaggg tctttttaca cgcagcggca acctcatctc
     3361 accatcgaac cggatatggg ttttgcctat tatgatgatg aaggcaggct taccatccac
     3421 tctaagagca ttgctttgta cctgcacgct ttaatgatcg cggatggttt gggtgtagat
     3481 gcctcgaata ttcggatggt tcaaaacact gccggcggca cttttggtta caagttttgc
     3541 cccaccatcg aagcgttggt tggcgtggcc tgcatcgcga cacaacgacc ggtttacctg
     3601 gaatatgatt atgaacagtt cattacctat actggaaaga ggtcgccttt ctttacctgg
     3661 ttgaaactgg gagcggataa agatggaagg cttattgcca tggaaactga ttggattgtg
     3721 gaccatggcg cttattctga atttggagac ctgttaaccc acaagggcgc tcagtttatc
     3781 ggggcgggat acgacattaa gaacatcagg ggaagagggc gaactgtgtg caccaaccat
     3841 gcctggggtg ccccgttcag gggttacggc gcgcctgaaa gtgagttccc ttccgagtct
     3901 ttgatggatg aactctcgga aaaaatcggc atggatccat tggaatttcg atatctcaac
     3961 gtgtatcgtc ctggtgcaac aactccaacc gggcaggtgc ctgaagtatt cacgttaccg
     4021 gaactgatcg atctcatccg ccctaaatac caggaagcca aaaaacgggc ggcggccgaa
     4081 tctactgcgg aaaagaaaaa aggcgtcggc gtcgccatcg gtatttatgg cgcaggagga
     4141 gacggtccgg atacagcaga agcctggatc gaaagaacgc catacggggt gttggtttcc
     4201 accacatgga gcgaccatgg acaaggcgct gacgctggcg cgctggggac tgcctacgaa
     4261 gcgctgcggc cattggggct gcgacccgag cagatcaggc tggaactgaa tgataccggt
     4321 aaagccccgg atggtggggc ggctggagga agccgcagcc aggtggtaac cggcaatgcc
     4381 attaaggtgt gctgcgagaa cttcctcgct gccatccgta aacctgatgg accttacctc
     4441 tcttatgacg agatgaaaga agacgacatg cccatgcgct attctggcgt ttggacttca
     4501 ccgaccgaga ttggtgaccc ggatacaggg cagggcaacc caattgcgaa ctacatgtat
     4561 ggcgtgttcc tggctgaggt tgttgttgac atcaagacag gcaaagtgac ggtagagaag
     4621 atgactctgg ctgctgacat tgggaagatc aataacaaag cggtggtgga tgggcagctg
     4681 tatggcggca tcgcgcaagg gattgggttt gctcttagcg aagattttga agatatccat
     4741 aagcacacga acatggtgaa atgtggtatt cctttcgcca aggatgtccc ggatgagatc
     4801 gagctgattt atttggagac gccgcgccct tccggtccgt tcggcgcatc aggggttggg
     4861 gaattgccca acacagcgcc gcatgcagcc atccttaatg cggtttacgc ggcctgcggg
     4921 gcgcgcatcc gcgagctgcc cgcgtaccct gccaaggtac tggcagcctt gcagcaaacc
     4981 agtcaataac cgccgggaaa aacctgtagg tatggcgtgg aggtcaatca tcgtacccat
     5041 acttgctcaa agcatctggt tgggttgaaa aatggatcaa cgccgtttac atgaacttga
     5101 agagcagtgc atccaggatt gtgacccgcc ttgcacttcc cgctgcccgg ttcacgttga
     5161 tgtacggcaa atgtgcaagg aaatttccgc tggggatttc aagcatgcgc tgatcacttt
     5221 ccagaaatca gtcccctttc ctgaaatcat cgcccgcacg tgcgaccaac cctgccaggc
     5281 gttatgcaac cgtgaacctt tgggcggggc gatacggatg gcagacctgg aattggcttg
     5341 ctgccaattg ggaatggagg agagcctgct ttcgcctgtg actgggcgga aaaatgccct
     5401 ggtctcggtg attggaggcg ggttgtgcgg catgacagcg gcttgggagt tagcccgcaa
     5461 aggatacagg gttgtgcttt ttgaccaggc agagcaactg ggggggcagc tttggggctt
     5521 gacggatgca caattaccta aggaagtcat cctgcgcgaa tctgcgaaac taaaaaataa
     5581 aggcattgac ctgcgtttaa aaacacgggt tgatctaaat gctttcgatt gggctcaata
     5641 tgacgcggtg ttgatcgcta ccggcgttga tcaagaatcg gtcaattggc tggagaaaaa
     5701 ccgtcagggc aatactgtga ttgacccggt gacatttgag tcgggtatga gcggagtgtt
     5761 ttgcggcggc agcctcttgc atccgccttc tacgataacc tccgttgcgg atggtaaacg
     5821 gacagccacc agtattgacc gctatttaca gaaggtgtcg ctgacggctt cccggtcatc
     5881 cgaaggaacg caagacaccc gcctgtacac taacctggca ggtgtgcttc cggtctttgc
     5941 tgtgcctcct gcggatgcgt tacaaggcta cacaatggag gaagcgattg ctgaagccag
     6001 tcgatgtctt gtatgcgaat gcatggaatg tgtgaaagtt tgcacttatt tatctgaatt
     6061 aggcgcttac ccacggaaat acgtcaggga tatttacaac aacctttcca taattatgcg
     6121 ccagaggcgg gggaataaat ttatcaactc ctgtacgttg tgcgggcttt gcggcgaggt
     6181 gtgccccact gatttgaatt tcgctgaagt catcgtgcaa gcgcgaaaaa ccatggtgga
     6241 aaccaaaaaa atgcccatct cagcgcatga ttttgcgtta cgtgatatga acttcagcaa
     6301 tggcccgcgg ttcgccctga cctaccccgc gcccggggaa ggaaaatgcg attatttgct
     6361 gtttccaggc tgtcagctgg ccgcgtcgaa cccagagtac atctccttga tctatgatga
     6421 cctggtcagg ctggtgggta acctgggaat catgctgcgt tgttgcggcg cgccagccca
     6481 atggtcaggg gaagagactc tgtttgccga aagcggcgct gtcatccgca gcgtatggga
     6541 atccctgggt aaaccaaaaa tgatcctggc ctgttccagc tgccatcaat cgcttaaaca
     6601 actgataccg gggttggaaa ccgtctcttt atgggaaatg ttattaaaaa atgaagcgct
     6661 cggaactacg cggggcgggt gcacgcagga atacaaggtc cacgatccat gcaccagccg
     6721 atacgagcca gattggcagc attatgccag ggcggtcatc gagcgattag gtgtccgtta
     6781 ttcagagctg cccatgtccc gcactctaac tgaatgctgc ggctatggag gggttgcttg
     6841 gttggccaac cctgacctgg tgcataaaat gatccagcgt aggatcaatg aagacaacgc
     6901 gaactatatt acctattgtg tgatgtgccg ggatcttttt gtatctgaag ggaagcctac
     6961 gcttcatcta ttggatttca tctatgggcg ggatatttcg gcgctggcaa taagaaaacc
     7021 accggattat tctcaaaggc acgaaaatcg catacgtgta aaacagcgga tggagaagcg
     7081 tttgctcgtg aaaggagact ggaagatgga aacttatgaa aagatcaacc tgcaattgag
     7141 cagcgagatg cgggatcttc tggaagcgcg attgatcttg gtggaagatc ttcagaaggt
     7201 aatcgaacac gctgaagcca gcggggaatt ttttttcaat aaaaacaatg gtcattccat
     7261 tgcctctttc aggcccaacc tcatcactta ttgggtggaa tatgagaaac aggagaattc
     7321 ttataccgtg tttgacgcgt acagccaccg gatggtggtg ggaggagata caagtatatg
     7381 acagcgtctg agatgaacaa aaactcgact gaatggatct gtggcaagtg cggagaacca
     7441 ttacgcttga acaaggttaa tgtgagttac ctcgggtcag gatatcccgt ggatttattg
     7501 acgtgcccca aatgcaaccg cgcgttggtc cctcctgaaa tggctctggg taagatgctc
     7561 gaagtcgaga ggttgctgga agacaaatga ttgcagattg cgccacctgc ggtttgttct
     7621 cctttcagcc gaaccctgga atttgcgtgc atacccaccg ccctggagga ttggtcatca
     7681 ccgaacgggc tctcgcatta tgcgatttac cagagcgggc gcgattactg gatgttgctt
     7741 gcggcgcagg cgcgacggtg aattttctgt caagtaaggc tgggttgaaa gtgacaggac
     7801 tggacaggtc tggcaaagcg ctctccggta ttcaaggtaa gccaatcctg ttgaacctgg
     7861 tgaggagcga tttactgaac atcccgatgg cgccagagta ttttgatggc gttttcatgg
     7921 aatgcgcgct ttccttgacg ggtgaacccc tcaatagcct gttggagatc aggcgagtgt
     7981 tgaagcccgg cggaaagatc gtccttacag atatctatct tagagaagtc atgaccgaaa
     8041 ccattcagcg tgttcttgcg ggcagcgctt gcctggctgg cgccatgacc caggacggag
     8101 ttgaagaggt gatcgcggcc gcggggttaa caaggttggt ttgggaagac cattcaccat
     8161 tgctgaaaca atggttggtt gaacatatct ttcgcctggg atcggtgtcc gggctttttc
     8221 agcagctcac cagggagaat gagcaaaacg agagggcagc gcaagcctta agccgcgtgc
     8281 gcctgggata ttacctggcg atacttgaaa aacctttgtc ttttaagagc caggaggctt
     8341 atgatggatg atctgtctaa aatccgcagg tttggcgaac agggatttta ctgcagccag
     8401 gtcatcctat taatggggct tgaatggcag ggaaaggaag accccgctct tgtgcgcagt
     8461 atgcactcgc tggcaggtgg aattggattt agcgggcata actgcggcgc gttgaccggt
     8521 ggcgcctgcc tgctgggata cttcgcaggc agaggttcta ccagtgaaga agaggacgac
     8581 cgtctttttc tgatgctgaa tgaactggtg gactggttta ccaggaaata tggagtcgaa
     8641 tatggcggga tagactgcga gaacattcta tcaggtggta aggatcagta tgtggctcgc
     8701 tgcccgggaa ttgtgcaatc tacactgcag aagtgcaagg agttattggt ggaatatggc
     8761 tatgagctca atggtgagtg aaatccctgt cgagggatgt gtgatttgtc gaacacagag
     8821 tatctgccct gtctgtttaa accggcttga agcgaaacgg gttgaatacg aggatggcgt
     8881 atacctggag aagcactgcc gcgaacatgg tttttttcgc accatcctgt ggcgcggaag
     8941 gccagattat ggcagttgga ggcgcgaaaa gctccccaat caccccaggc acccttttac
     9001 acaggtggag agaggttgcc cttatgattg cgggttatgc gcagaacacc gccaggaacc
     9061 ctgctgtgtg ctgctggagg tgaccagccg atgcgacctg gactgcccgg tgtgcttcgc
     9121 ttcttctgga gagaaagacg ctactgatgt gccgttatcc acaatctcct tgtggtatga
     9181 acgtatgctc aaggcgggag gacctttcaa tattcagctt tcaggaggtg aaccctgtgt
     9241 tcgtgatgac ctggcgcaga tcattgccat gggaaagaaa gcgggatttt cctattttca
     9301 gcttaacacc aatgggctgc gcatagccag ggagagagat tatctcgcca gcttgaaaga
     9361 ggctggtctc ggcgtggttt atctgcaatt tgatggcaca acagacgaca tttaccgaac
     9421 cattcgagga agagaaattc tggacgcgaa aaagaaggtt atcctcaatt gccgcgcgct
     9481 cgaattgggc gttgtactgg tgccgactgt tgtgccggga gtcaacgaca aagacttggg
     9541 aaatattgtg agattcgccc tggagaacta cccggtcgtc cgcggtattc attttcaacc
     9601 gatcagttat tttgggcgct ttcctgacca gctgcggaat gaagatcgta tcaccatccc
     9661 tgaaatcctg cgcaatttgc aagaacagac tgaaggatta gtcaaaactg aagattttca
     9721 ccccaagggc agcgagaact catactgttc ttttcactgc acctacattc ttatgcctgg
     9781 gggcgagcta ctggcggtga aaaaccaggc gggtaaaacc tgcggctgtc gagagcctga
     9841 ggattccagt aaggctttat tacgaaccag gtcatttgtc gccaggaact ggatatatcc
     9901 cacgcctagc gctccttgtt cttgtggcga gtcggcttcc atgggagagt gggatgtcat
     9961 tctggagcgc tcgaaaacgc atcttttttc tgtttcagga atggcttttc aggacgcctg
    10021 gaaccttgac ctggatttgc tacaagactg ctgtatcaat gtggctgctg atgatggaaa
    10081 attgattcct ttctgcgcgt acaacctcac gaacacgctt ggagagggtt tataccgcct
    10141 gtaaggccac atggaattga agattactcc cctacatacc tggatttcag cgcgtattgg
    10201 cacccctcgg gccgcgtttg accgccaggc gctggagcga tggcagttta gcagcctgct
    10261 gcgaatggtc gcatataccc ggcaaaaaag ccctttttac cgcgagcatt ttgcagggct
    10321 gggaccaatc aattcatttc cggatttttt caggctaccg ttcacaactg ccgagaattt
    10381 gatggaaaac ccccattcgt tcgtatgtgt ctctcaagag caagtccatc gtattgtgtc
    10441 attgcccacc tccggcacca cagcgaaatc aaaaagggtt tttttctctc aggaagacca
    10501 ggcgctgacc atagactttt ttcgaacggg catgtccacg ctggcaggtg caggggacag
    10561 agtgctgatc ttgctgcctg gcgagaggcc gggcagcgta ggagacctgc ttttcactgc
    10621 cttgcgtgaa ctgggctgct tgcccaccaa atatggcccg gttgatgatg aaaggagtgt
    10681 attgcaggtc attcggcaag aaaaagtgaa catactggtt ggtgccccag ttcatctgta
    10741 ccgggtggcc tgttttgacc atgtgactaa ttttctttcg caagggcaaa taaaagcggt
    10801 attgacctcc acagatgtgc tgcctgtggt tgtggcggag aggttgaagg ctctctgggg
    10861 atgtgaaata tttgaccact acggcatgac cgaaaccggg ttgggcggag gagtcgagtg
    10921 cagcgcgcat tgtggttatc acatgcgtga agcagacctt tacattgagg tcattgaccc
    10981 cctcaccggg gaggtactac cagacggaga agaaggagag gtggtgatta ccacgcttac
    11041 acgcaacgcc atgcccttga tacgctaccg taccggcgac ctcagccacg tgatacccgg
    11101 ggattgtgcc tgtggttctt ttataaaaag aatagcaccc atcaagcgcc gctggcgtgc
    11161 gggtattcag ataggcgagt gtactctttt ccaaaacgac ctggatgagg ttatgtttca
    11221 actgaatggc gttggtgatt ttgaagcatc tgtctttgtg gatgaacaag gccgagacat
    11281 gatgaggttg tctctacgca taatgggtgg aaaaagggag cgtgttcaag aagaagcccg
    11341 ggaagtggta tcgcgtatcc cgggcatgga ggaattatca tgcaatggca ggttgttaat
    11401 tgaagtggtt gatgcgctga acccggtatt tgccaaaaga agaattattg acctgcgccc
    11461 aaacgtacaa catcccacac aggccagccg ataagccgca ttctgttccc tgaaaaggta
    11521 tgcgcgtcgc acggtttcca ggtagatggt catctctcta ggccgggtgt tgccaccagg
    11581 ctcgtgcagc ctacccggga ctcataagga aacgggccgt ttcccgccgc cgtagcggac
    11641 atggtccctg cttggccttg ctccggttgg gggttacctg gccgctgcat ttcggcagcg
    11701 ccggtggtct cttacaccac cttttcaccc ttgcccttac gggcggtatg tttctgtggc
    11761 cctgtccggc gggttctccc ggaggatccc cgccccgggt gttacccggc aacctgccct
    11821 gtggagtgcg gactttcctc gacgctgatc gctcaacgcc gcgaccatct ggctggcctg
    11881 atacttccat cataccttct cttaatcccc ccgtcaatct taaaaaaacg tccaggaggt
    11941 ttaataagag taaaataaca gggcaaggat gtaccagacc cttgctggaa ggtagaggat
    12001 aaagaagaga ttatgcgaaa accattggtc gccggtaact ggaaaatgaa taaaacggtt
    12061 gaacaggcaa gtttgctggc tcgagagctg ttaacgggtt tacagcaggt gcctgaagtt
    12121 gagcgggtgc tttgcccgcc atttacctcg ctgatgatcc tttcggccca attgagcggt
    12181 accggggtag ggattggcgc acaaaatatg cattgggcag aatcaggcgc gtacaccgga
    12241 gagatatcac cacaaatggt gcgtgagttt tgcgaatatg tcattatcgg gcattctgaa
    12301 cgaagaacct attttggcga gacggatgag agcgtgaacc gtaagctgaa atctgctctg
    12361 gctttgggac tggttcccat tgtgtgtatt ggcgaaacgc tggaagaata cgaggcgggt
    12421 gtaaccggcg aggtaattac ccgccaggta cttgaagggc ttaaggatat aggaacggca
    12481 tcggcttcaa tcatcgtggt ggcttatgaa ccggtatggg ccattggcac cggcagagca
    12541 gccaccgctg aaggagcaaa cgcggtactg cgggatcata tacgaccggc gctggcacag
    12601 ttatttggct cagggctggc acagaatctg cgcatcctgt acggtggttc ggttacggtg
    12661 gccaatgcta ccgaattttt ccgtcaaccg gaaattgatg gcgcgctggt tggcggtgcc
    12721 agcctcaagg tagatgaatt tgtgaaaatc gtggaagccg caaggccata attaagaaca
    12781 gaacaacaga agacctggct gttggctggg tctttttgta ttttacgcgg tgccaaattc
    12841 tcgatcgccg gcgtctccca aaccgggaag aatataaccg acgttattta agcgttcatc
    12901 taccgcagcc aggtggatgg gcacctcggg atgtgccttg gtcatcgcag caatgccctc
    12961 gggagccccg ataatgccca cgaacttgat cctcttcacg ccccagcgtt tcagcacctc
    13021 aaccgtcgct gtggctgaac ctccagtcgc aagcatgggg tctaagatca ggcacacgga
    13081 gacggttggc tcagttggca atttgttgta atactcgaca ggcttgagtg tgttctcatc
    13141 gcggtaaatg ccgatatgcc agacctcggc gccgggcatt agatcccaaa aaccttccac
    13201 catccccagt ccggcgcgta agatagggat gagaccaatc ttgtctttta aaatcttccc
    13261 agaagccgtg gcgagaggag tggttacctc tatatcttcc atcaccaagt cagcggtagc
    13321 ttcgtaagcc agcaagccgg caatctcacg caccagctcg cgaaacttct tgggttcggt
    13381 gcgggcatca cgtaattttg ctaatttatg agcgaccagg ggatgagcgg acacatgaag
    13441 gttggacata gcggctcctt gaaaatttat atcattatag cgccaaataa aaacagcgtt
    13501 caacaaaatg gctcagttga tcccttgcag gcaggggagc gtgtataatg gggtttatga
    13561 cagcagacga acgcatcgtg aaccctgtgc caaaggtgga cgatcgggta gataacgtgt
    13621 tgcgcccttc caaattgaag gaactcatcg gccaggacca ggtacgccaa aatctggaga
    13681 ttttaattgc ggctgcgaaa gggcggaagg aatgcctgga ccacgtgctg ttttacggcc
    13741 ctcctgggct gggaaaaact accctggcgt acattctggc aaatgaaatg ggggtcaaca
    13801 tcaaggtcac ttcaggtccg gctattgagc gcaccggtga cctggcagcg atcctgacca
    13861 acttgaggga aggtgacatc ctgtttgtgg atgagataca ccggctgggt cgctctattg
    13921 aagaggttct ttacccggcg atggaagatt tctcattgga tatcatcatt ggcaaaggac
    13981 cttctgcacg ctcgctgcgc cttaaattgc cgcgctttac caccattggc gccaccacgc
    14041 ggctggcgtt ggtcaccgcg ccgctgcgcg cgcgctttgg cgctgtttac cgccttgatt
    14101 actacgacct gacagccatg cgtaagatcg tagaacgcgc tgcgggttta ttgaatgtga
    14161 ccagtgatga taccggaata gaagaaatcg cccgccgggc gcgaggaacg cccagggtgg
    14221 cgctgcgctt gttgcgccgc gtgcgcgact tcacccaggt gcgtgcaaat ggtctcatta
    14281 cccgagaaac ggccatcaat gcgctggatt tgctggcggt cgacgcgatg gggttggatg
    14341 atgtggatcg cagagtatta aagaccatta ttgaaaaata taatggcggt cctgtaggcc
    14401 tggcaacaat cgccgcttcg ataagcgaag aggcagacac aatcatggat gtggttgagc
    14461 catatttact tcaactggga tatatcgacc gcactcccca gggtcgagtt gccacgccgc
    14521 tggcttacga acacttgcaa ttgccttata accagaagga taaccagccg cggctgattc
    14581 cttgagcatg cagaccgctg attttgatta tcacctgccg ccagagtaca ttgcccaaac
    14641 gccaatcgaa ccgcgacacg cgtcacgttt gttggtgtta cggcgttctt ctgccaccct
    14701 ggaagacagc cacttttgcc agctgcctga ttttctaaat cctggcgacc tcctggtgat
    14761 caatcaaaca aaggtgatac cggcgcgttt acacgcccgc aagaccagcg gcggcaaagt
    14821 ggaattgttg ctgctcaaac agcgggagcc gggtttatgg gaagccctgg cgggaggtaa
    14881 aggattgcgc aaaggggtgc gcgttcagct ggagaatgga ccttatgtgg acattgaaga
    14941 ggaactgacg ggaagcgaaa ggttgattcg cttttctgag ccagtggagc aattactgcc
    15001 aaccatcggg gagatgccgt taccgcctta tatccatgag aaattgctga gacctgaccg
    15061 ctaccagacg gtgtacgcaa ctaaaaccgg ttcagcagct gcgccgaccg ctgggctgca
    15121 ttttaccccc tggttgatgg atgaaattga aagaaaaggg gttcgtattg ccaaagtgac
    15181 gctgcatgtc gggttggata cttttgcccc ggtaacagag ttggacccgg ttgaacacaa
    15241 aatacacacg gaatggtgcg agctcaccga agaagcggcg ctgctgatca accgcacgaa
    15301 agcatctggt gggagagtgg tggcagtggg aaccacgagc gtgcgcacgc ttgagagcgc
    15361 cgcggctacc agcaaccagc cctcttgggt agcgccctac cgtggacaaa cagggctttt
    15421 catccttccg ggatttgaat tcaaagtggt agacgcgctg gtcacgaact ttcatttgcc
    15481 aaaatccact ctgctgatgc tggtgagcgc cttcgcaggg cgggagcgag tgctggatgc
    15541 ctaccaggcg gcgatggagc gcgggtaccg cttctattcc tttggcgacg ccatgctcat
    15601 tctttaggag actcattgac ctgtccgcgc agcctgaaat tattggataa ccaaatcatc
    15661 gcctgcagaa cctgtccacg gctggttgca tggcgggaaa aaattggtca ggagaaacgg
    15721 cgcgcctacc tggggcaaac ttactgggca aaaccattac caggttttgg cgaccccctg
    15781 gcgcgtgtac tggtggtagg cctggcgccg ggagcgcatg gaggaaaccg caccggacga
    15841 atgtttacag gtgacgcttc aggcgacttt cttttctcag cgttgcaccg ggcagggttt
    15901 gccaaccaac ctgaagccaa ggcattgaac gacggcttaa tgctcaatga tgtttacatc
    15961 acagcggttt gccgttgtgt gccaccggat aataagccct tgccagctga gatctcaaat
    16021 tgccttccat acctgcagta cgagtttgaa ttactggaga aagtagaagg cgtggttgtc
    16081 ctgggcagga tagcgtttga cacagtcagt aaattgcttt atcaccagcg caaattggcc
    16141 acctcggcga tttttgggca tggggtcctg cagccatcca ctgatggtgc cccctggttg
    16201 ttgtgctcct atcaccccag ccggcataac acccagaccg gcaggttaac taccaccatg
    16261 ttcgatcaga tctgggaaca ggtgaaccgg ctgttgggaa aagatgagtt actttcttgt
    16321 caggaaccag gctcgtcctg actggcttcc taacggggag atgggatcca ggctgctgac
    16381 tttatcaacc acgtgaagcc ccgcatattc cagacaggat tcgatctcag cgattgaaaa
    16441 accgcgttcg atgtgctttt cttcaaattt ttcccactta tcatccactt tgacaaacca
    16501 ggtgatcttt atacagccca ggttcttttc gaaatcataa caagtgcgat gaagttcgag
    16561 caagtccttg gtttcttgtc gaacataaca gcggttcttt tgccactcca ccgctaaccc
    16621 ataaattgtg ttcatatcaa aaaggaacca accattgggt tttaaggagc gcgcaatgct
    16681 gttgaaggtg gattgcaggt cgttgttgga aatgagatag ttgagactat caaaccagca
    16741 agtcgccagg tcaaattcgt tgtgatagtc aatgaagcgc atatcctgtt tttcaaattt
    16801 cacatcgacc tggtcatttt ttgcccgatg ttttgccaga cgcagcattt cttcagattg
    16861 gtccacgccg gtgactgacc agccattctt tgccatttcc acgcaaaagc tgccctcacc
    16921 gcaggcgaca tccagtaatt tctttctgtt attcgtgagt attttgtagt gcttcatcac
    16981 ccctggaaga atagcagcca tccgttgtga gaggttcgga taatcaccgc tcgcgtagac
    17041 actggcaaaa ctgtcatata gtttcatctc tctccttgtg gggcctccca aactggcagg
    17101 ttcatcaatt agtaccacac ttggcgggaa ttgaaaatgt tacggattct ggatgtgatt
    17161 acaactctgc aaacaaaaag acagcggtgg aggccgctgt ctttgaatga atgaaaaaat
    17221 taataggctt tgagcaccga ccacccagcg taagcggcgg tttcacccag ttcagcctcg
    17281 atgcgcaata attggttgta ttttgccaca cggtctgagc gggcgggggc gccggtttta
    17341 atctgcccag tgttgagcgc gacagccagg tcagcaatgg tagaatcttc ggtctcacct
    17401 gaacgatgag aagtaaccgc ccgccagccg gcacggtggc aagcttcgac ggcttcgatt
    17461 gtttcggtca gagagccgat ctggtttact ttgacgagca gggcgttgca ggcctcttcc
    17521 tttatagcgc gccttacccg tgacgtattg gtaaccagca ggtcatctcc cacaatttgc
    17581 accttgtgac cgatttcttt ggtcaacaac ttccatgaat cccaatcatc ctgcgccagc
    17641 ccatcttcaa tagacacgat gggatactgt ctgacccagt tcaaccagaa ctccaccatc
    17701 tgttcgccac ttaatttttt gccctcctta cgcaggttat acgttttggt ttcttcctca
    17761 tagaattctg atgcggcagg gtcgagcgcg atggccactt cttcaccagc cttgtaaccg
    17821 gccttttcaa tggcttcaag gataacctca accgcctcgc tgttggcttt gagagctggc
    17881 gcgtagccgc cctcatctcc aaccagggta ggatatccgc gagatttcaa cacactttta
    17941 agcgcctggt aaatttcatc cccgcaacga agaccttcag caaaggatgg cgcccccagc
    18001 ggcatcacca tgaactcctg cgcatcggta gattgccatc cagtgtgggc gccgccattc
    18061 agaatgttca tcatcggtac tggcagtaca tgcgcgtata ccccaccaat atagcggtag
    18121 agcggcagat cgagtgacgc tgctgcagct ttggcgactg ccaggctcac acccaggatg
    18181 gcattggctc ccaggcgaga tttattgggc gtaccatcca attcaatgag catctggtcg
    18241 atggctttct ggtcgatggc atcccaccag atcaggtttt cagcgatttc accgttcaca
    18301 tgctcaaccg cttgcagcac gcccttgccg ttatagcggt ttttatcgcc gtcacgcatt
    18361 tcaagcgctt cgtgaatccc tgttgacgcg ccggagggaa ccgcagccgt gcctattgac
    18421 ccatcttcca gtatcaccct cacttctaca gtggggttcc cgcgtgaatc gagtatctct
    18481 aatgcttcaa ctgatgcgat ttccattttt tatccttttc cctttttcag gtgtggttaa
    18541 ttgattttga gcccaaacgg ctgtaagtat aacgaaaaat ctctatttgt gggttaccca
    18601 ccgaggagtt gccgaagttt tttaaccacc cggagtgtcg attgaccgtc attgtatgtg
    18661 catatttctt caacgatctt cttgcgccct tcggttcttt cagctggctt ttcgaagcca
    18721 tcattgaccg cgcggatgaa atcgggcctg gagtaaacca ggtccaaccc cccggaattg
    18781 gtgatgggaa tgtagtgttc gcggatatac agttccttca gcacgcggtc atacttccgg
    18841 ctgctgccgg ggtcataggc gggtccgatc tgagggcggt cgaatacagc cccatccacc
    18901 gtgagggtag aagaagagtt gatctgcacc gcgcaatgcg ccagtgtgga acagagttgt
    18961 tcgatatcgg catgggtgat gttgatctgc ccacggcttt cattggcttg ccagctttgg
    19021 tcagtaaagg tgttttttac cccgctcaac acctcttgcc agcggctggc gatatcccct
    19081 gggtgacggc ggaacagaat gataggatgt gcaggaacct ctccattcga aatggcgtca
    19141 tccagttgct ttaagaagag cggctcattg ggaacgattt tatatgtacc gcccccgaaa
    19201 agaataaccg gtcgcgcaga tgggagcccg agcctggaac gccatttttc ttccggtatg
    19261 acgtttgcag aatcgtagta aaaatcaaac tgcggaggac cggaaatggt gatctgttca
    19321 ggagtgatat cagggtatgc gcgcaacagt tctgcgtggt tgtagcggtt ccaaaccagg
    19381 taatgctcaa aagaaacagg caggcgggta cgactggtga cgttatcaaa cgaaaggatg
    19441 gaggtaattc gcaaaacgcc gcgcttttgc gcgcaacgca ataacggttc ttctttgttg
    19501 acaaaaggag taatcgaaaa gaccgcatcg ggtttggact ggtcaagaat ggaatcaaat
    19561 tcctggtaat tcgtctccga ccaaaacagg gattcttcct gctgcaggag gtgcgggatg
    19621 cgcgccggga gggctgtttt gattctatcc ttcagaattt gaacctcgta gttcaccctt
    19681 tcccgcaaac ccagcagaga ggcttcacgg cggatatcaa tctcagtggt gttggtctgc
    19741 cgcacctgtt tatgccagaa gtttacaagt tctcgggttt ctttgaattg ggggctgaag
    19801 gtgaagttgg gaacatgaag aatttcagcg ccagcctggc tgaattcatt ttcaagcgcc
    19861 gggtcttgcc aacttaaagc cacgatcggt tgagtgaatt cggtgagcgc tgtcaacata
    19921 ccggttctga gaatgtagcg cacggaaaag tgggtgaaga cagggattaa taacctgggt
    19981 ttgggcatac cgactccggg acttggcttt gttaccttgt ccatgtaaat tgaataacct
    20041 ggatattata tccgcttatg cgaggattaa cgtttgtaca acagcggcgg gtcgcgatag
    20101 gccactccgg ctttcacagc cgcggcgcgc aggaaagcga ggaaaagcgg gtgcggtcgg
    20161 gtaggtcggg agaggaattc cgggtgaaac tgtgtgccta gcataaatgg atgatctttc
    20221 agttccacaa tttcgaccag gcttttatca ggcgatagac ccgaaaaaac catgccgtgt
    20281 tgcgccatcc gttctcggta ggcgttattg aattcaaagc ggtggcggtg gcgttcttcg
    20341 acctttttca cgccatacgc attggcagcg atggtgcctg gctgcaggat acagggatag
    20401 agccccaggc gcatggtgcc gccaacatcc ttaatgttct gctggtcagg catcagatcg
    20461 attacggggt gcggggtgct caggtcaaat tcggcagagt tcacctggtc agaacccagc
    20521 gcgtacctgc cgaactccac gcacattaac tgcatgccca ggcaaagccc aaagtacgga
    20581 atcaagttta cacgagcgta attggcagcc caaatcttcc cttcaatgcc ccggctgccg
    20641 aatcctcccg gcacaatgat tccgtgggcg tcttccagag catttacatg ttttgctttt
    20701 tcaagatcag ccgaatgaac ccacctcaaa tccagttcca gacccagaga gagcgcggcg
    20761 tgttttaagg cttcacgcac actcatgtag gcgtcatgaa gctctacgta cttacccacc
    20821 agggcgacgg aaaccctggg tttttcacgc gctgtttcgc tgaccagttt ttcccagtca
    20881 ctcatatccg gctgttgttt ggaatccagg ttcaggcggt gcagcaggta ttcgccgact
    20941 ttttcttttt ccagcagcag agggatttcg taaaggatgg gcgaagtgac cattgggatc
    21001 acggcgcgtt tttcaacatc acaaaactgc gcgatctttt cccgcaaatt ttcagcaacc
    21061 gggtgatcac tgcgggcaac gatcatatcg ggggcgatac caatggagcg caattcagcc
    21121 acggagtgct gggtgggctt ggttttaatt tcatcagtgg caccaatata aggcagccag
    21181 gtaacgtgaa cattgacggt gttttctctt cccaggtctg aacgcagctg gcgaagagct
    21241 tctaaaaagg gtaggctttc aatgtcgccc acggtgccgc caacttctac aatgacaatt
    21301 tcggcgctgg ttgatttgga caccaggttg atccgacgtt tgatctcgtt ggtgatgtga
    21361 gggatgacct ggatcgtgcc acccaggtaa tcaccgcggc gttctttcgc gatgacttca
    21421 gcataaacct gaccagtggt gatgttgcac acccggttta ggcggatatc aataaagcgt
    21481 tcgtaatgac ccaggtcgag gtcagtttca gccccgtcat ccagtacata aacttcgcca
    21541 tgctgatatg ggctcatcgt tccaggatcg acattaatat acgggtcgag tttttgaacg
    21601 gcgactttat acccgcgttc tttcaggatc aatccgatgg ctgcggcggt cacgcctttt
    21661 cccactgaac tcaccactcc gccggtgaca aagatatatt ttgtggtcat ggtctgctcc
    21721 tgaatgagaa atataaaaga agcgaggagg gcggtttatc cttgtcctcc tcggaactca
    21781 cttgatgtaa cccaatatct cgcgtgaaat atcagcattt gtcttttctc aataaaagtg
    21841 tatcacaact cgattttcct gacaaaagaa aaaccatcca gaaggatggt ttttttgaat
    21901 cagttggttt gaactttgtt ttccagcgtg cgggcgagga tttcatcctg ctcggtggag
    21961 ccgggtttcg tcctatggac ttcctgtttc tttttctttt gtttggcttt ttccattgcg
    22021 gcacgcatcg ccagctccat ataggtgggt tcaggctcta cagtctcgtt cttcacttcg
    22081 agaaccccgg agctttcaac ttctgatttt cgacgaggtt tcctggcgga tttcttctcg
    22141 cgccgcgctc cctctgcgcc ggaagcagcg ggagctggtt tttcttcaac gaccttttca
    22201 ggctctggtt gaatggcctt catacttagt ttgatctgtt tcttgcgccg attgacctcg
    22261 ataatttggg cttcgacctc atcgccttcc tgtaccacat cagatggcag cttcacataa
    22321 ccatgcgcca tctcgctgat gtggacgagc ccgggtcttt cagcgccaat ttccacgaag
    22381 gctccgaact tctccaggcg aacgaccttt cctttgacag tttcgcccac cttgatttca
    22441 cgccactcaa gatctaaggg cttgaacatg gtcatttcaa tacgttcttc gctatctttt
    22501 cgggtgagct ttttgaccca cacgtcgatt tcttctccct ctttgaggag ctcgtcgacc
    22561 ttcttgttct gtgtgttatc tggagcaatg atctgcgaaa cgtggagcaa agcaggtttg
    22621 ccgatgccga tatccaatac agcgccggcc aggctcacct tgattacccg tgctttgaac
    22681 ggcatttttg gttcaagttg ctgattgtct tgagtttctg cgttcatttc catcaatatt
    22741 ctctttcatc tcggtagtta gcgaggaaaa attatacccc ctgcagggag gaatgtcaat
    22801 tttttctcac tgttttgaga aaccaattta cttgttcgcg ttcaacagca ggtcaatcgc
    22861 cttttccagt tgaggatcaa gttctgcttc tatatcgttc tctgtgcgct ccacaatgta
    22921 atcaggtgtg agcccaattc cgtggatgag cctgccctta ggggtcaacc aacgagcgat
    22981 ggtcactttt acggcaccct gttcatcagc gaggggcacc cagttttgca cagatccttt
    23041 gccgtaggtg gttgtgccga caatttttcc gcgctgatag tcttgtatcg cgcccgcggt
    23101 gatttcagag gcggaagcgg aatactggtt aaccagtacc accagtggta tctcggtagc
    23161 caacccgcct ggatgcgctt cgaatgtgac ctctttacca tctccgaacc tttctatcat
    23221 gatcacccct gacccaatga actgcgagat gatatcgatg gcggcgtcga cataaccacc
    23281 gctgttatcg cgcaggtcta agatcagccc gaccgggtgt tgtgccaaca atcctgccag
    23341 cgccgtttcc atttcttgtg cgctttcgct tccgaatgtg ttcagggcga gataaccgat
    23401 gccctggtca agcatgcggc tttcaacact ggaaaccgtg atctgtgcac gcacgatggt
    23461 gaaatcaagg atttcttctg gagcatggcg ctgaacggtt aagactacgg tggtgccttc
    23521 aggtcctttc acggtttgga gtaccgcgct tgggtcctta ccaatcacac tttgaccatc
    23581 aaccccgatg atctcatcgc ccggcttcaa ccccgcggct tcggcaggag aaccagacat
    23641 ggagcggatg atggtaaggt actcaccggc tgtgtccacc caggcgccaa tccctccata
    23701 cgaaccgtcc aaagttgtgg tcgcctggtc gtaatcttcc ggggtcatgt acccggtatg
    23761 aatgtcaccg agcgaattga gcatgccttg aatggcgccg cgcatcagtt gaaggtcgtc
    23821 taccggttga tcaacatact gctcgtgaat gatgtcccac gcttcccaga aaggttggaa
    23881 caattttctg agatccgcgg gtgtagatga aggcggtgtt gaatcaggta aggggggcgt
    23941 ttctgcaggc tttgttgtat caggcgcgag cgtctcgagc ggagaaccag agggaatcaa
    24001 ccagccaagg ataacaccgc cggaaaaggc accggctaac aacacaagtg aaatcataat
    24061 gaccaggatg atcctaccgg tgtgattggt gttcattttt ttactcctgc ttcatggtta
    24121 ccattctttg aatgttcgac gggataagtt ttggccttaa gatgctgaac gcgctttgaa
    24181 agttcgtgta ttttcttgtt ttcttcggcg aaagggtcgc gcagcgtgtc gcctgctttt
    24241 ttcactgtgt ttgcccaatt tgagcgctgc cctttctctt tcaacctgga aaataacgag
    24301 atgaacaaac tgatgattaa catgaccgta aaaacaatca ggatccaacc caggagtggt
    24361 attggttttg ccaaggttca gcgctccatt gtcctgtgcg aagaggggat cacatcaggc
    24421 aggtcaagga gagatagtat cgctgcgtca gcaaattcgc acctgttttc cgttcctacc
    24481 tgtatcgtgt aaaagcccat ttcatgggcg gtctttaaat ttcgcggcga gtcatcaatg
    24541 aatacacagt gttcagggtc ttcaatttga atgatttcca tggctttctg gtatgcctgg
    24601 ggcagcggtt tacaatacgg tgatatctga agaacatcga caatctcagg gaagaaatcc
    24661 acaagtccca ggacctctaa aacccgctca gcatgccttt ggttggcgtt ggtaaagata
    24721 accttacggc tgtcataggc ggaaatcgtt tcaataagcg cttcatcttt gcgcaagaat
    24781 tgcgtaagag ggatatcgtg cacgtaatcc aggaattcac attcgtcgat gtgataaagg
    24841 gtctttaacc cgcgtaacgt ggtgccatat tcgtgaaaca gcccattgcg gatttgttta
    24901 acctcgtttg cggccactcc gagtgttttt atgatgtacg aatccatacg atcgccgatg
    24961 gcatcccaca cgccactagt agaagggtaa agggtatcat ccagatcgaa gagcattaca
    25021 gcaaaactca tagcggttag tataccctca aattgattcc ccggtctgga ttaacccccg
    25081 ataggggtca gttataatta gaccgatgag catacaggtg ctttctgaag aactggcttc
    25141 acaaattgcg gctggagaag tggtggaaag accggcttca gtggtgaaag aattgctgga
    25201 gaacgccatt gatgccggcg cgtcgcgcat ttcaattcgg gtagaagacg ccggtaaaaa
    25261 aaagatagaa gtaagtgatg atggaactgg catccctatc gatgaattac cactggccgt
    25321 ctcaaggcac gccaccagta aaatcaatac cgcggaggat ctttttcata ttcgcacgtt
    25381 gggcttccgt ggagaggcgt tggcatctat cggctctgtc tcccgcctga tggtcttgtc
    25441 gcgtaacgca gattcccagg ttggcgcgcg catcatggtt gaaggaggga gaatcgtcag
    25501 caaggaaatg actggcagcg taaccggtac aatcgtgcgc gtcagcgacc tgttttacaa
    25561 cactcccgcc agattgaaat ttctcaagcg ggatgtgacg gaaagacagc agatcgatac
    25621 cctggtgagc aggtatgcga tggcgtatgc cggcaaagcg attcatttag agcatgatgg
    25681 caaagcggta ttacggactt cggggaatgg tgagcggcgg gaagtattag cgcagctgta
    25741 tggaattgaa ctggcgaggc agcttctaga agtggtcttt gaagaagaag ggatgaagat
    25801 cgagggttat atcagccccg tctcgatcag ccgttctaac cgtaaggata tgaccttttt
    25861 tattaatggg cgctgggttc aggatgttac cctggtgagc gcgctgttaa aggcatatca
    25921 aacctacttg atggttggac gatacccgct ttccatttta tttattgaga ttcctccaga
    25981 agcggtggat gtaaatgtgc acccggccaa ggctgaggtg cgctttagag agccagaccg
    26041 tgtatttacc gctgtgcaac gcgcggccag gagagcgttg atggcttacg ttcctatccc
    26101 gccactggcg gctgcttcct ggagcagccg ggggtggaac ccttcgcctg aactggatgg
    26161 cacgtggatg aatgccagac ccgtgcagcc gcagagtact ggtcaacccc aaccgatggc
    26221 tgaaacacct ttatcatcat cggtagaacc agcgcctttt gcagcaggga taccattgct
    26281 gcgtttggtc ggccagattg gcgccactta cctggtggca gaaggcccag atgggcttta
    26341 cctgattgac cagcacgcag cgcatgaacg tgtgctatat gaaaaaatgc agcgacaaaa
    26401 agagaacatt cctacgcaac ctttgcttga accattgctg attcagttac ctccccaatc
    26461 cgctcaaatc atctgtgata accttcaggt gttaaggaag attggttttc atcttgaaga
    26521 gttcgggcgc aattcctttc ggctgagcgc gattcccgct ttgttcaccc aaaaagatcc
    26581 ccgggcagcc atcaatagcg tggtggaaga ttttgaggaa gatgaagaac ctctcaagga
    26641 ggtgcacgaa gacaggctca tcgcgcgaat ttgtaaacgc atggcagtta aaggcggctc
    26701 catcttgtcc accgaggagc agcaggcgtt gttgcgcgac ctcgagagtt gttcttctcc
    26761 gcgcacctgc ccgcatggtc gaccgaccat gattcacctt tcagttgagc tgcttgaaag
    26821 acagttcgga cgccggggag cccgttccgc tttttaacca caacgctggt tgagcaatgc
    26881 taccatcgcc gggatgcatt ccgccacatc tgaacgcatc accaactccg cgtatggatc
    26941 aatggaagtg gcagaatgat taaccaggat aatcttgcat ccattgcggc acgccttgaa
    27001 gggcagtagc gcggcgggag taacctccaa cgaagaaccc gccaccaaca acaaatcggc
    27061 tagcgcgatt tcattttcag ccttcagcca gtcatccgcc gcgagctctt cttcataaag
    27121 ggtaatatca gggcgaagga cagtgccaca ggtagcgcaa agcgggattt cacccgagag
    27181 gattgacgtg acgactgcgg cgaaatcaac accgtgttcc ttgtttgcgg ggcagtaaaa
    27241 acgatgaatt gaaccatgca gttcaactac gttgaccgag ccggcttttt gatggagttc
    27301 atcaatattt tgggtgatga cagcctgtaa tttgccggct ttttccagcg tagcaagggc
    27361 gaggtgggca tcattcgcat gggcactata agagacccgc agcaaggggc gcagccagtt
    27421 ataaaaattt cgcggcgatt ggcggaatgc cgtggcagag gcgactttca ttgggtcatc
    27481 acgctgccac aaacccgtcc ccgcgctgcg aaaatccgga atccctgaag gagtggaaat
    27541 acccgcgccg gtgaacgcca caaaatattt tgaactggcg atcaaatcag cagccctgcg
    27601 cagttcgtgc tcaactggtg tggtcacaat tgaaaccgct ggcaaatcgc gcgggagagc
    27661 tcattgacct gctgagaaaa caacccttca ggttggatat gatacagcgg gatatatttt
    27721 tgattcgctt gaaaaacctg ctctggaaca ggtggaatca tgaacttgat cgtggtacct
    27781 ggcagtaact tggttatctg ttcagcagta aggcccgcct cagaacggat gtgattatag
    27841 aagaccgtgt cgatgtaccg gtttttcttg ggtacctcaa actgcaatgt tccaagcaat
    27901 tttctggtac gtaacaccgt tgcggggaat gcgtctgtca gcacgatcag ctggtcacaa
    27961 agcttatgga ccttgctgaa acctgccagg tagggattgc cgatatccaa taccaacaca
    28021 ggaattgagg aattcaatga tttgatgatg gcgagggttt tttctggcag gtcaggcagg
    28081 ctgatttttt gactcttttc tgaagccatc agcaggcgga cattaaatga ggtatgcacg
    28141 gctaattttt caaccgctgc tgctgtgatc tcgtcaactg gcagtgcgag aagcttctcc
    28201 aacccatagg aattggcaat atccagttcg accccccaac tgccctgacc tggcttcagc
    28261 tccacggcta ccacctcttt gctggtcatg ccactcaggc tgacagccag gttcaatacc
    28321 agtgttgata cgccaagccc gcctttgcat ccaagtaccc cgatgataca acctgaaccg
    28381 ctttcttcgg gcgtattttt tatttttttc atacgatcga gcaaggcttt gacatgggcg
    28441 ttgagttcag cggggtgcac tggtttggtc aggtaatcat ctgcgccggc gccgtaacca
    28501 gcgattttgt cacccatctg gtttttcgcg gtaaacatca ggatggggat atgggtgcac
    28561 agggggtttt tccgcagttc gcgggcaatc tgatagccat ccatgcccgg catcacaaca
    28621 tccagtatga tcaaagaagg ttttactttg accgcagtct ccagcgcaag ctcgccggtg
    28681 tttgcagcag caatttcgta gccctgacgc tgcaacatca tcccgacgag acgcagtgtc
    28741 tccatatcat catcaacgat gagaattttt tccggcatgc cgaccacccc tcctgaactt
    28801 ataagccagt ctagcatatt ccctgacaag attcaagtga ttattttcgc ttcaagttga
    28861 gaaaaaatac cctgaaaggc aggcgtatat cagcaatgct ataatgacgg tgtgaatatc
    28921 caggaatttc aggcaatctt gcagaccaga tgcgccattg acttaaagaa gccggttttg
    28981 ttgggattct ctggcggacc agattccatt tgcctgctat cgttgctggt gaagagcggg
    29041 gtggaggtta cggccgccca ccttgaccac gcgttgcgcc cggactcagc cgctgaagca
    29101 gtacaggcgg aggagatttg ccgtcaactg ggcgcggatt ttatcagccg acgagttgat
    29161 gtagcggatt acgcccaaaa aaagcacctc tctgtagaag aagctgctcg taagcaaagg
    29221 tatgcatttt tattcgcaga agcggcgaaa accggcgcgc aggctgtgct gaccgggcac
    29281 caggctgatg accaggttga aaccgtgctg atgcaccttt tgcgaggaag cgccttgagc
    29341 ggactggctg gcatgcgcat ggtgctgctg cccaatccct ggagcaaaga catcccattg
    29401 gtgcgtcctt tgctcgcgtt ttcacgagag gaagttgaag cctatctttc agaaataccg
    29461 atcagaccca tatttgatca atcgaaccag gattcgagct ttttccgcaa ccgcatccgt
    29521 aatgatttgc tccctcagct actcacctat aatccccagt ttaagaaacg catcctcagg
    29581 atggccgatg cgattgcctt ggaagacgac ctggtcatgg ctcaaacccg gcaagcctgg
    29641 gcggaaaccg ttatcgaaac gggcgaggat ttcgtggttt ttgaccgagc gaaaacggcg
    29701 tctctgcacc ccgcgctacg ccgcaggttg atgagggaag ggcttaccag gttgcgcagt
    29761 gaactgcggg acatggatta ccaggtgatt gaaaaaggca gccatttctt tacagatcca
    29821 tgccagagca acactgtaca cctgatcgcg ggtttggaga tgtttgcgca tcttaaaagg
    29881 cgtgtggtgc tggcaaaaaa agaatctcct ttacacgggt tatggccgca attgactcag
    29941 aaaaaagcgt tacccctgaa gataccgggc atggtctcgc tcaatgagca ttggaccctg
    30001 cgcagctctc gtgaaacaat ttacataaat actggtgacc cttttgtttg ccagttgaac
    30061 gctgaattat tggctgataa actgaccatc gatcgtttta aagcgggtga caggttctcg
    30121 ccttatggcg ccgctgccca ggagatgaaa ctgggcgact tttggaccaa ccagggtttg
    30181 gcggcgcgcg cacgaagtaa ctggccactc attcgcagcg gtgaggagat tgtgtgggta
    30241 cctggatttc gtatcgctga ccgatggcgt gtaaaaaaag agaccacgag cattctaagg
    30301 ctcgagttaa taaaaacaga gtgacagcct gttgctgcca ctctgcgcgc aacaacccgg
    30361 tcattctcct gccagcaggc gcaatttatt gctggtctca cgccattgaa tcttcgaaat
    30421 gcccagtttc tggcgcaagg cttctttatc catccctgat ttccaatcat tcaaggcgca
    30481 ggtccagcgg cacatggcga atgaaatgtg tttagatagg ccagcgtcga tggtgatatc
    30541 ttcaagcaag tactcaaggc ggcgcggaga ccaggggaaa acctgttcga cgggatggta
    30601 tacatccaga tattcctggt aagcctcgac ccaatcctca ttcagttgga tattgcgttc
    30661 cttgtaacgg ctgccgctgg ctgaataacg cacaaacaga cgcggactct tcgggctgtc
    30721 aaggtcgata tggttgaggg tgagtgcgag gcactcgccc tttttaattc cggtggacaa
    30781 caacaggctg agcaaaacgt atgggcgggg atcgggttta ctggcaacac gcagcgctct
    30841 ggcggcttcg agggccaaca cttcttcttc aggtgtaagc acgtctggca aggggctgat
    30901 taccgtcttc tgcagcactt tttcagccgg gtcagacggg atacgtccgg aacgtgatag
    30961 ccaacgaaag aaggatttag tagaggtgat ccttcgcgac aggctttttg cgctgcatgg
    31021 cacgtggcgt tcatgctgaa gccagccaag gaaacggttg atgtcatctg tggtaatact
    31081 cccaatggtg gtgccagaag ggaaatagtt ggcaaataaa cgcatgtctc ccaggaaggc
    31141 ttttaccgta tgaagagaac gcccctggtc gagcaagaat atctcccatg agcggatggc
    31201 agggtgaaaa gttgtttctg aagtgatgtg gagttcaggt tgaatgtcca taaaggagcc
    31261 ttctttcaat tattaatttc gcatagtcct attaatatca gtgtacaagg attattggcg
    31321 aatgtcaata tgcaattaat gttccaaaga ggggtataag gcgtcttgga tcgttcgggc
    31381 aatcgaatca tagtcaggca acagtaccat ggcacctgtt gcaggcacaa tatagtttgt
    31441 cgtttgatct tctgtaattg tgaagcgatg gatggaggag gggttatcag cagcggtggc
    31501 tgccataggt aaaagccgcg caattaaatc aagcgggata tccgtctcca cactggaaag
    31561 aaaaagttgg tagagctcac cagcacgatt cagggcgttc aggctgatca ttttttgggt
    31621 gatggctacg gcaacttcct gtgcgcgccg ggttctatca aaatcgcttg tggaataacg
    31681 tgagcgcgcg taccataagg cggtctctcc attcatcgtg ttccaacctg ccgggatata
    31741 acacattttt gcgtaagcct gtggcaggtc gcaacggtca tacaggtcgg aagttgcgta
    31801 tacctgaatg ccgcccaggg tatcgatgat gtttttaaaa cctgaaaagt cggtcatcat
    31861 ataataatct acactggcat caaaactggt cttgagcgtt tccgcggtga gcgggaaccc
    31921 cccataagcc tgggctgagt tgagccgctc gtaaccaatc cccggaatat tgacgtacag
    31981 gtcgcgcgga aaagataata atgtcactcg gttactatcg ggtatcagcg aaaggatcat
    32041 gattacgtct gtgcggtaac cctgcccggg tcgccagtca gaacccagca ggaggatgtt
    32101 cacctggctc gcgggtcgtg ccaaccccaa tgcaatcatt ccacctgtac ctggtggagg
    32161 agtcactgag ggagtgctgg tgaaaacaga tgggggattt attccctgga aaacgggttg
    32221 gaagggggtg ggggtagcgg tgaatcggcc gccttctcct gtcaacaagt cagagatcgg
    32281 aataggggtg tttcccttta ggtccgtggt gaaagttgaa caggcagaaa ggagaagaaa
    32341 gaacacaaat aatgacgcca gcagaagcct gcgcattgcg ttattgtacc ccaatcgcgc
    32401 agttctattc agagcattgt ttttaccagt ataaaaaatg aaaattgtgg agcccctacc
    32461 agtaattcgt attttctttg ttatatgact tgacatatat atatctattt gatactatat
    32521 cattatagat aaggagaaag ggtattcaat gacacttaaa cacgcaatcc tcggattgtt
    32581 gaattatgag ccaatgacag gatatgacct gaaaaagacg atcgacatct ccatcaatca
    32641 tttctggcca gcggttcagt ctcaaatcta cacaaccctg gcaaaaatgg agaaagaagg
    32701 ctgggtaacc gtagagacaa taccgcagga gtcactccct ccacgcaagg tatattcatt
    32761 aacccctact gggcgagcgg aattcgtgga ctggttgaca actccccagc cgtatggaga
    32821 aacgaggata gcctggctgg tgcaggtgtt ttttgcaggt caactggatg atcagaaagc
    32881 cttgctactc ttgcacaacc taaaatcagt ttttcgtcaa cggcttgaag ggtatgcgtc
    32941 catccctgaa gacaaccgcg attcacttcg aaatgaccca ccacgagata aattcttctg
    33001 gatgctgaca gtcgatttcg gggtcgccca gacgatctca cagatgcaat ggattgataa
    33061 agtcattcgc acggttgaat cgcatgaata tattttacct acccttgcgg gcgatcgttc
    33121 ctaatattct tttttataca tcaggagaaa ataatgaaaa agttattagg agccggaaac
    33181 aaaccctggc attttattgc gggtattttc cttagcctgc tttctgccgg aatgctgctg
    33241 ctggccttcc atccatataa ccaatggtac ctggcttttt ttgccctcat tcccatgcgg
    33301 ctggcagagt atcgtttctt cccgcgtaag tgggctggtt tgggttccgg gataggcgtt
    33361 ggggtttggc tggcggtttt cctgggacgg atgttcggct tcagcggccc tgcactggtg
    33421 attgaaatcg tggtgctcgc aattgtcctg gtgcaggttt tcaccattcc cgcaacccgg
    33481 caattccacg aacgtacggc ttaccgctgg ttcatcttgg aaggcatcgc aggctgggta
    33541 gggtctgaaa tgattcgctc atttatcccg cccatcaaca cccatgcctt tattgcccag
    33601 accatgtaca cccagccatg gatgctgcag ccgatctcca tctttagtat ctatggcctt
    33661 tccaccctga tcgtgcttgt caattacact ttacttcagg gtatcatctt atttttggat
    33721 aaaaagcagt tgttttttaa tttaccagaa atgaaccggc gttccacctc ccgctggctg
    33781 actgttgcgg gtgctacctt gatcctctgg cttgggatca gtggtgctat ccttttgagc
    33841 cgacctgcca atccaacaac tgtccgggtg gctgctgtcc aactgggaat ctccgcccct
    33901 gctcaccagg atacggctga atcccaatcc acccgactgg atgtattggc tgagttaagc
    33961 cggattgccg ccacagaagg cgctcagtta atcgtttggc ccgaacttgg tttgggcttt
    34021 gacccgcaag tggagcatac cggggagctt atatccctgg ctgctgaaac tgacgcatac
    34081 ctgttaatcg gctatggatt ggatgacccc cgtggctggc gaaatgagat ggtgatgtta
    34141 tcaccgcagg gtgaattcct ggcagtctat ggaaaaaacc atcccacctc tcccggagag
    34201 cctcgcatca ttacagccgg agtgtacccg gtttatgata cgcccatcgg caggctggcc
    34261 accatcatct gcaacgatgt gcatttcacc aatacttcac ggcggctggc acgcgctggt
    34321 gcccaattga ttgcagtgcc tactctggaa atctccggga ttaccctgga acaggtcgcc
    34381 cagagcgtga tgcgggcggt ggaaaaccgg gtggcagtgg tcaaggcgga cgtcgctttt
    34441 tcttcagcca ttatcgatcc gtacggaaag atcgttgccc tgcgtaacgc ttccccgcag
    34501 ggtgcagctt tcaccctgac tgcggatgtg ccgatcggta gcgggaaaac cgtttacaac
    34561 tggctaaacc tggattggat gggctggctg aacctggttg gtatggtatt cttctctttc
    34621 ggtgcaaaat ggttggagaa aaaagtccgc tgattatcaa aatattaaca atctatcttc
    34681 atccaggtgc aaagcatcac tatagaagaa aacagatatt ccactcacct ggctgcgaaa
    34741 gatccgcgga ctggcgggtg tcaagcatat cgttaatgca gatcagtact catttttgat
    34801 attaataaag gagaattcat gaatcacaaa gtgggtgatg ctgggtggat caggtctttc
    34861 tatgattctg ctgttgggtg gtggggagag agttggtatg agggagaaaa tctaaagaat
    34921 agattggata tcgtaaggaa gtatgcaagc aatatagata aaagaattct ggagttggct
    34981 gcaggcacag gtgagacggc tgcctatttt tgtgatcatg gctatggcgt ccttgccgtt
    35041 gatatctcgc attgtaactg tgaattgatg atcaatcttc aattaggcaa atctaacctg
    35101 cgggtcgttg aaggcgattt cctgaaattg gatatcaagg aacagttccc tacggtctgc
    35161 atgtttgagt cttttggatt tggaagtgat caggaacagc aacaattgtt gaaaagggtg
    35221 aatgagaact ggcttattcc aaatggtgtc ctgatcctgg atgtctacca cccggcagga
    35281 cctatccgag cgtgtggcaa aaagcaggaa cttgacaaat tggaaaacat cgctggttcg
    35341 gtggatatga ctgaatattc gtattacgac cccataaaga gtcgctggat agatatttgg
    35401 gagccaaggg acaacaagga aaacaggaga gtgcaatcca tccggtgtta cacacctgcg
    35461 gattttgtct tattggcagc gggatgcggg ttatctatcg agaaaatgct atatcgtggc
    35521 atggagtttg accatgaaac tccagaagtt acaaccgaaa atattttcga tgatgatagt
    35581 aattattcct acacggtaat tctgagaaaa aaggaatagt acaagtctaa actaattctt
    35641 gagtatggtt cagttcaacc attgtcctcc gggtcgcgaa aaaactactt ctgccacagt
    35701 actgccacag gttgacattc ttcaggagtc tgttaaaata tccctcgtaa ttgccgaagt
//
LOCUS       DAOTLW010000018        35229 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1458_length_35229_cov_11.457753, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000018 DAOTLW010000000
VERSION     DAOTLW010000018.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 35229)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 35229)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..35229
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1458_length_35229_cov_11.457753"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1250
                     /locus_tag="P5516_04360"
     CDS             <1..1250
                     /locus_tag="P5516_04360"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT91703.1"
                     /translation="FKKIAQEFPRKFWSVVGVSFIDRVGGTMLFPFFALYITQKFHVG
                     MTQAGVVLGLFSVFGLVGQMIGGALTDKFGRRKLIIFGLVFSAISTLAFGFINTFSAL
                     IPLAIGVGLLSDIAGPAHAAMIADILPENQRQEGFGILRVVGNLAWIFGPTIGGFVAG
                     KSFLALFIIDAVISSLVALLFYLLIPETKPQNQPGEKPESLLVTFKGYFQVLRHGAFI
                     AFMAAGMLMGLVYQQMYHSLSVYLRDNHGIDPKGYGFLLTASAIVVILFQFSMTRWIK
                     KKPAFKMMALGTLFYMLGFSMFGFVGQYWLFVLAIMIITTGEMIVVPTSQALAVNFAT
                     EDMRGRYMGVFGITWMLPATIGPGAAGLILDHFNPNLLWYIGGVLCAISALAYYGLHL
                     KIGAQERFQPAQDEPAAVEDPAV"
     assembly_gap    1259..1358
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1359..2225
                     /locus_tag="P5516_04365"
     CDS             <1359..2225
                     /locus_tag="P5516_04365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91704.1"
                     /translation="SLSACNLPINAGHSGAISQTEIALNVALTQFAASQTAAVPPTAE
                     PTATVAIPTAAPEVLSPPNTPEPAFTPTRGGVWLTVQENTNCRSGPGSLFDFMGLINA
                     GQQVEAVARSTVGEYYYIKNPTSYSDYCWLWTRYTTVAGDTGTLPVFTPQPSPTPKVT
                     PTFTKAPADFLVKYLNTQSCSGLYGIQLYIENTGSTTWQSIRVRLDDTTNAVTTNHKN
                     NYFFGTDGCVKDLSNRQGDLTPGEYSRVALVDDGELSYNPSGHSFTAQVTLYSGDDQT
                     GVSVTKTITFTP"
     gene            2261..>3108
                     /locus_tag="P5516_04370"
     CDS             2261..>3108
                     /locus_tag="P5516_04370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HRT91705.1"
                     /translation="MIKRIFLTITLLFIAAFTLSACQGGPTPVAPLEQSDLQTLVAGT
                     LTAAAVEMQQTMTAAVTPTETIAPTLTETATLPPTDTPTKEVVSITLSGNTNCRRGAS
                     TYFPVITVIPAGSVVEVIARNPESDYFFVRAAESDTGGCWIWKEFASVTGNLESLAVY
                     TPVPTPLPTATNTPPPGANFTAQFVGLTACGTGYAANFNISNTGTSTLQSIRIKITST
                     SWNFTFIDNTGFSQWSHGSKYATLAEITSGKSMIVSTCPGGFNSSPSGQDIKAEITVC
                     LNDNLTG"
     assembly_gap    3109..3208
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<3209..5100)
                     /locus_tag="P5516_04375"
     CDS             complement(<3209..5100)
                     /locus_tag="P5516_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012869.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91706.1"
                     /translation="MMHGIRRLAATEEKLPNMTWALVKRVFAYARPYRLPLLVILLLI
                     SASSGLNLLNPLILREMIDRTIPQGNVRRLVLLAVTLLLVALLNGAISVVNRRLNASV
                     GEGVIRDLRVSLYAHMQRMSLRFFTNNKVGELMSRLNNDVVGAQNAVTNSVISIVTSL
                     VQSIAVLVVMFTLDWRLTLISMLVAPFFAVVAALLSRRLRSIVRKQLEANASMNAMMN
                     ETLNIGGTLLVKIFGRQAQEVERFSRRANQVKEYGINRAQTGSIFGAIIGLLVAVGTA
                     LVYGLGGYFVISGVFTVGTIVAFSSFMLSLYGSVQSLVNAPVDFASSFVSFERVFEVL
                     DQPVEISEKPDAIALTEVEGEIAFENITFAYQAGDGVILSDVHRYGSMDSIVTTLSDG
                     ESELEPEERRSQARGNALENVSFHVKPGQLVALVGPSGAGKTTLTYLIPRLYDPSSGR
                     ITLDGTDLRDVTLESLTRQVGMVTQETYLFHDTIRTNLLYSRPDASQAELEEACMAAN
                     IHDFVRELPDGYDTVVGERGYRLSGGEKQRLSIARVILKDPRILVLDEATSSLDSEAE
                     ALIQEALKRVMRQRTSIVIAHRLSTILAADLILVIDRGRVVEQGNHASLLAQKGLYAT
                     LYQTQFR"
     gene            5418..6053
                     /locus_tag="P5516_04380"
     CDS             5418..6053
                     /locus_tag="P5516_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HRT91707.1"
                     /translation="MQSKPKLAIFDFDGTLADTRPFMLSILDHLAERFNTRRVDIHSL
                     EQMRGYTARQLMELYRVPMWKFILMARESQRLLYQNITSIQLFPGVESALRAIARRNV
                     AIAVVTSNQLRNVLAVLGEELAGLVSVFECEAGFFTKARKLRSALRQAGVKPGEAVSF
                     GDESRDIEAARKVGIPCAAVTWGYAHSEVLRHHQPDYLLTSVDQIMGIVAE"
     gene            6222..7013
                     /locus_tag="P5516_04385"
     CDS             6222..7013
                     /locus_tag="P5516_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRT91708.1"
                     /translation="MGYIMEALHYLLSGLGAFAAGAVNALAGGGTLITFPLLTALGLP
                     AVSANITSTVSLFPGYFGATWAQRRDLGGQRARLIALLPAAALGGLIGGLLLFYSGEK
                     TFKQLIPWLILFSSLLLALGEPLRQMLKRSPHPHPQRRSPRLWLISLPVCLAAIYGGY
                     FGAGLSVILLAILALFLDDSLTRLNGLKQFLAFAANSGAAIFFLFSGRVNWRVAGVMA
                     ISALLGGIAGGKLAGRVSPVVLRWVVVSIGVVVAVIFFIQIYLKP"
     gene            7028..8215
                     /locus_tag="P5516_04390"
     CDS             7028..8215
                     /locus_tag="P5516_04390"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT91709.1"
                     /translation="MLNTKPRLALIIACSMYLAFGMFNAAIGPVLGELAENTHSTLVM
                     VGGVITFLFLGSLATQLIAGPIIDKFGLRIVGLVSLLLVAFGLPAFTNVHSYPLMLAL
                     VLLTGLGQGGMDLTANLLVADAYPSKSTPMLNLLHFFFGLGAFFGPALVGLAIVATGS
                     GMIIHWVAAGMFLLLALILLRVPAGKAPAPDATKKPDAKVKDEKSIYLSPFLCTIGVL
                     ILIYVGTEYGLGSWSTRYMGLTTGMADQNGALVTSAYWGALTLGRLAGAAASRRLSNT
                     RLLGVSLGGSLLGTLGLVLSRGSVLSTVVFIVITGFSLGTVYPTSVAVTVEKFPKDQG
                     KTVGLLAAMGSIGGLALPWLAGFLLETTSALVFALFLCATTVAMLLLLFVLGQYKKGT
                     SNG"
     gene            complement(8286..9620)
                     /locus_tag="P5516_04395"
     CDS             complement(8286..9620)
                     /locus_tag="P5516_04395"
                     /inference="COORDINATES: protein motif:HMM:NF012783.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HRT91710.1"
                     /translation="MKTRWFLQVSLILSILVSFFQPLQLVSGQVDAPEAVVVQLKGPL
                     TPVWSGYLQRGINRAVSTGAGLLIIELNTPGGSVDLMNHLVQQILASPVPVVVYVTPR
                     GAMAASAGTLLVLSGHFAAMAPESTIGAASPVGMQGEDIGSTLEEKTKEILKASARSL
                     AARRGTEAVKLAEESIEKARAANSTEALQAGLIDFIAHDVEDLLAQMDGSKVLMNGNE
                     VVIRSKGALQVPVSTTMVEDVLGLLTNPNIVFLLLSIGVQAILIEISSPGGWVAGFIG
                     AVLLALAIYGLGILPVNWFGMIFLVIAFVLFILDIKAPTHGALTVAGTGSFIAGALIL
                     FNSLSIPGFPRVSVGLVIGMGVFLGLSFFAVVMIALRAMKKPVITGRESLEGKDGYVV
                     TRLAPIGIVQVAGEQWSAVLAPGEPPLKKGEKVIVEKVEGVRLVVRHIETKA"
     gene            9768..11660
                     /gene="dnaK"
                     /locus_tag="P5516_04400"
     CDS             9768..11660
                     /gene="dnaK"
                     /locus_tag="P5516_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRT91711.1"
                     /translation="MGKIIGIDLGTTNSVVAVMQGGDAQVINTAEGARLCPSVVAFNK
                     NGERLVGQTAKRQAVINPENTVFSIKRFMGRRFDEVESERKMVPFEVISGPMKDARVR
                     IPVTGRDYSPQEISAMILSKLKADAEAYLGEKVSQAVITVPAYFNDSQRQATKDAGKI
                     AGLEVLRIINEPTASALAYGLDKKKNEKILVFDLGGGTFDVSVLEVGEGVFEVKSTSG
                     DTHLGGDDWDEVIVNWAADQFQADQGIDLRKDRQALQRLREAAEKAKIELSTVMETEI
                     NLPYITADANGPKHLVTKLSRSRFEQMTAHLLDRCKKPFESALKDAGLKASDINEMVL
                     VGGSTRMPQVVDMVRGLINKEPNKGVNPDEVVAIGAAIQGGVLGGEVKDILLLDVTPL
                     SLGVETMGAVMTPIIERNTTIPIRKSQIFSTAEDGQTAVDIHVLQGERPMAADNMTLG
                     RFRLEGIPTAPRGIPQIEVTFDIDANGILNVTAQDKATGREQKVTITASTNLQKSDIE
                     RMVEEAKAHKSEDDRRKELAEARNIADNAAYQVEKAVKEAGEKVTASDRSSIEARVQE
                     LRSAMTGEDVSRIKSLTETLQQDYARVMQASAQAQTAPGGGDAGGSAPPDDGGDVVDG
                     EFTEKN"
     gene            11790..13148
                     /locus_tag="P5516_04405"
     CDS             11790..13148
                     /locus_tag="P5516_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coproporphyrinogen III oxidase family protein"
                     /protein_id="HRT91712.1"
                     /translation="MLEFSHSYYSQANMSASVPSTFTPTPIVRVAERLLTAFLKVAAK
                     QYLSLQPTNLSSVPPPQPGHRYTLYAHVPFCESLCPYCSFNRFVLDREKAAAYFKSLR
                     AEMRLVANLGYRFSSLYLGGGTPTIVPDELARTIDLARELFSINEVSCETNPNHLNPE
                     MVTLLKERVQRLSVGVQSFNDGLLKQMNRYQKFGSGASILESIRRTAPHFKSLNVDMI
                     FNFPSQTPQILRDDLHAVIDSGAQQVTFYPLMSSPSVRRSMASTVGELIPAREADYYA
                     IISQGLAQDFSALSAWTFARNGSNLIDEYIVDNEEYVGVGSGVFSYLNGCLYASTFSL
                     QEYSDLVSQGRMALSGAQPFSRRQQMRYRMMMDMFGLRFDRAAFRQRFGVPASRGLWL
                     EMLFFRLLGAFEKKNPAIPTPVGRYLSLVIMREFFTGVNQVRDIARAALSPYDRGECY
                     AP"
     gene            13548..14888
                     /locus_tag="P5516_04410"
     CDS             13548..14888
                     /locus_tag="P5516_04410"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRT91713.1"
                     /translation="MRYRRKWLLLILVVLIVAGLTLSLPPVWSRITYHTHNLFADIKY
                     RLNPPQAEVFVPSGAEEGNLIATASAATLTALVPTPTSPILTTEETRLPSSTPTLTPV
                     PLPIKVYLPGVKPEPQLWNNCGPATLSMNLSFYQWGKTQVEAAEVLKPNPRDKNVMPY
                     EMVTFINTQTSLRALSRMGGDVETLKRLLNAGFPVLVEKGFEPGDLKKEGWMGHFNLV
                     IGYNDDAQSFITQDSYLLSHPPGGGEIKDLETFPGFTVPYEDMQKDWRAFNYVLIVVY
                     PPEKENDVLNALGPLMDENGAYRLAYERAKMEATTLSDIRERFFAWFNAGTSLVYLAD
                     YNGAAAAYDSAFNLYAQIPENARPWRMMWYQTGPYFAYYYSARYTDVINLADQTLKRM
                     SAEPILEESYYWRGMAYLALGDNERARAEFRDSLKYHPGFGPSLVALEQMGETP"
     gene            14925..16163
                     /locus_tag="P5516_04415"
     CDS             14925..16163
                     /locus_tag="P5516_04415"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HRT91714.1"
                     /translation="MIKILHFADAHIDIATHGKHDPETGLPIRALDFLKALDTIIDHA
                     ISEKVDLVIFAGDAYKDRTPSPTFQREWGKRIIRLPQADIPALLLVGNHDISPASGRA
                     HTLQEFDTLQVPKVRVLYKPELLKPADLWGLQLQVLSLPWVFSSSLVAALGLSAVEED
                     TVHEEIEKRLTTILQARMDELDPALPTVLVGHGSVQGAVYGNERTVMLGKDLVLPGSL
                     VKDPRLDYTALGHIHKYQDLNPGLHPPVVYAGSIERVDFGEVADDKGFVIAEVEKGNT
                     SYRFVKLHGRAFFDRLVRLSMEDRAMEKILASSPNEAEAENAIFRLVIEYPRELEADI
                     DEPALRQKFASALEFHLVRRPQAEARLRLPADQNIASYSPLELLSTYWKSTHAQPRDL
                     AGLQTLAQSIFQSVTGSKPG"
     gene            16184..17512
                     /locus_tag="P5516_04420"
     CDS             16184..17512
                     /locus_tag="P5516_04420"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRT91715.1"
                     /translation="MSNTLIEILVIFVLILFNGFFSMSEIAIVSARKVRLEQRADEGD
                     AGAGRALQLSKETGQFLSSVQVGITLVAMLTGAFGGATLAERLTQSLARVPWLATSAA
                     TLSLVIVVLVTTYFSLVIGELIPKKIALNNPEKIASKVSGIMVFFSRLTSPLTRLLSS
                     STDLGMRLLGIKPSGEPPITEEEIKVLIDQGTQVGVFGEAEQDMVEGIFRLNDRSINA
                     IMTPRTDVTWLDADDSIEEILKTILQSPYSRFPVSRESLDNVLGILKSRDLLEKQLSG
                     EPFNLLDLLQKPLFIPENTPVMRVLEMEREHGVHEALVIDEYGGVLGFVTLFDVLEAI
                     IGELPSQDQASEPEIVMREDGSYLIDGLLPIDEFKDLFDLDLLPEEDKVGFQTAGGFV
                     MNQVGSIPHAGQTFQWNDLLFEVLDMDGLRIDKILVSRNSEKTPPAGGDK"
     gene            17509..20073
                     /locus_tag="P5516_04425"
     CDS             17509..20073
                     /locus_tag="P5516_04425"
                     /inference="COORDINATES: protein motif:HMM:NF024573.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HRT91716.1"
                     /translation="MIIDRLQISGFLSYKEKVDIDFSGFALACISGQNGAGKSSLLEA
                     ITWALFGEARRKDDAIIHMGTDTAEVLLEFDYESTRYRVQRSKTRDKSTQLEFQLRNA
                     EGNWVPLTEATMRGTEELIRRTLHLDYETFTNASFFLQGKADQFAQQRPGDRKRILSG
                     ILGLDIWEDYREEAARRRKLLENDLALVISHLQEIETELQQEAERKERLARLEKEVST
                     QTELFEARKAYLEQQRQVAERILADQKQVEKQRAEINRLRAEDETMRSDLDLRTQERQ
                     THLSLLQQEKEIRQEVDAWEQARKTLEEWDTLAQDFHQYDSQRQAPLQAIAVEESRLQ
                     AELNSLQQKQAEIFSIEQELPEIQRLVEDSSKAVEDCSAKLATRAQLEKEMRALLDEI
                     ARARAENTRLKMEMDEIVEHRTHLENISGANCPTCEKPLSDDERQRLVDEINARGKEK
                     GDAHRENKRIIEQSEAQYREIETALLNLQRVDAELKLQQRIFDTKAQELRMKEAAFTD
                     WQQKGAVRMLELEETLRSQSFALEARKQLAMMDAQLKKLGYDAAAHEQARRMELDGRA
                     SQDKKIQLEKARSALAPLEQNITSLQKNMENNQKRLSALLDELRQDEEKLEIETSNLP
                     DLDQLERDYYAAQEQMNQANRDLGYTRNQVEVLEKQRELKVEKLEEKDQLNNQVTDLK
                     TLERAFGKDGIPALLIEQALPEIERHANEILDRLSSGSMSISFETQRDFKDKKREERR
                     ETLDILIRDPAGERPYELFSGGEAFRVNFAIRLALSRVLAHRAGARLQTLVIDEGFGS
                     QDADGRQRLVEAINLVSTEFEKILVITHLEELKDAFPARIEVTKTPAGSKVEVIAV"
     gene            20070..21170
                     /locus_tag="P5516_04430"
     CDS             20070..21170
                     /locus_tag="P5516_04430"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT91717.1"
                     /translation="MNMIHARVGLQQRVLPEYRAEFFDTLATACLQGLGVFAGEPCQG
                     EAVEVVDQLSIAYYTHARNLHFFRGSAYFYWQAGVLGWLQRWQPDVLIMEINPRNRSN
                     PLAARWMKERGRPVLGWGLGLPGAGKIYRLIYHSLLRSCDAVIAYSTTAALQYRHAGV
                     DPSRVFVAANAVTRRPTDLVVARPPSFHENTPQLLFVGRLQPRKRVDTLLYALAMLPP
                     HQQPRLSIIGDGPDRSRLEMLAWQVYPRAVFHGAKRGQELDPYYQAADLFILPGTGGL
                     AIQQAMAHALPVIVGAADGTQIELVREENGWTLPDDSPETLARVIREALSDVERLRQK
                     GLASRRIVSEEVNLEGMVKTFCTAVESVLSKA"
     gene            21182..22360
                     /locus_tag="P5516_04435"
     CDS             21182..22360
                     /locus_tag="P5516_04435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT91718.1"
                     /translation="MNLLIIADGRSPITRRWIRMLQPLNYTINLVSTYPCQPVDGVNE
                     TILFPVAFAGLSGSQAGGGARGGRKVVSRYRGLAATLRHWLGPLTITHAEKDYLELLN
                     RLQPDLVHALRIPYEGMLASRTPTGIPLVISTWGNDFTYHAISNGVMGSATRRALLRA
                     DGLISDTVRDLRIARAWSFNPAKPAVSVPGNGGIDLAEIENITQGIDPAVPWQVINPR
                     GFRSGSVRNDTFFKSIPLILSLYPEVQFICPWMAGQPEALSWVNRLRILDNVSLLPMI
                     SQAELWREFARSLVSVSVSEHDGTPNSLLEAIAIGCLPVCGDIESIREWIRNGENGIL
                     VDPADAYALAEAVQRGIMDEEFRRAAAARNREIIRERAEITIVRQKVAEFYQRMTGFV
                     "
     gene            complement(22402..23508)
                     /locus_tag="P5516_04440"
     CDS             complement(22402..23508)
                     /locus_tag="P5516_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HRT91719.1"
                     /translation="MNSKVAVLKTTPETVIEDYIRLFELADGPKALDPGATTILKDNI
                     SWHFPMPSANTTPWQMEGTILALKSKGYNDITCVQNQTVVTNAFKGEDLNGYVPIFKH
                     YHIPVLYNFEDKDMTWVKYKPKAKMNALDHIYPEGIYIPDYFVGKNIVHLPTAKCHIY
                     TTTTGAIKNAFGGLLTKHRHYTHSYIHETIIDLLAIQKEIHTGLFAVMDGTTAGNGTG
                     PRVMKPEVKNVILASQDQVAIDAVAAKLMGFDPMSIKYINLGHNQGLGVGDPREIELV
                     GDDISGENWHFKVGHNLHQFMGWLTWYGPTKFLQKLVTRTPLVAIPIAFSEVYHDYVH
                     WPLKEKYVFKEWCQNTGWGQLFMRYKKEGVIHNL"
     gene            23536..25173
                     /locus_tag="P5516_04445"
     CDS             23536..25173
                     /locus_tag="P5516_04445"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT91720.1"
                     /translation="MGELYQQAVRLANPACVRATPADLVYNPSEFMNKKMPLKQPGWN
                     FRSRACGLLICGLLLLASCNFPIRDFSALPRPYATLDPAIFEYHRSATPTAGQPGAAP
                     TRTPQPPPVVDESLYHIYTIRSGDTFETVARHFSVTPAAIISMEALPQTGILPPGRYL
                     VIPKNTDNLFEQEMILPDSAVINSPCAAPFDISAYVSAAGGYLSTFSQPVAGIRSSGA
                     EIVRRVADNQSVNPMLLLAILEHRAGLVFGKQPPADINHPLGFRNMYYKGLYQELSLA
                     ARMVNTGYYGWRYGTMTMVTFTDQVSQRISPHLNAGSVALMYLFSQSYPSTDWNERLL
                     GKSGFMETYLAMFPDPWLCAAEVEPLLSDTLSAPQLELPFAPGEEWAFTAGPHYSWVE
                     GTPLGALDFAPNIKSAGCRVSPSWVRASAPGVITRADNGVVILTLEDEKGDVTGWEVM
                     YLHIAANERVPLGTRLRKDDPIGHPSCEGGSSTGTHVHIARKYKGEWIGAQGLFPFVL
                     SGWVAYPGERAYQGRLMKDGQVVIARQGGNQDSLIKR"
     gene            complement(25267..25758)
                     /locus_tag="P5516_04450"
     CDS             complement(25267..25758)
                     /locus_tag="P5516_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HRT91721.1"
                     /translation="MNAKVYEAMNDQIKHELYSAYFYLSMSAYCETENLPGFAKWLKV
                     QAEEEQEHAMKLYDYLLDRGEKISLKAIEQPPVEFKGVKDVFTKVYEHEQKVTGLING
                     IYAKAVEANDVASQIFLQWFINEQVEEEKNASSVLAMVTKLEGSLGGLYQLDHQLGMR
                     EAD"
     gene            25935..26072
                     /locus_tag="P5516_04455"
     CDS             25935..26072
                     /locus_tag="P5516_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91722.1"
                     /translation="MAENLCMLLEFLSTRALQDLETEMIDKTLPAGASPRLGVAVCQS
                     A"
     gene            26514..27266
                     /locus_tag="P5516_04460"
     CDS             26514..27266
                     /locus_tag="P5516_04460"
                     /inference="COORDINATES: protein motif:HMM:NF013757.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HRT91723.1"
                     /translation="MTEIQSLARGLRILLMLSDSLDGSTVTEVAENLGVDKGSASRLV
                     NTLVKYGFAEKDPVSRRISLGPQIVSLSRSLLTRMPLREVAKPYLRELMRSTGECAHI
                     GILSQGKVLYIDQVESPASLRVNAEVGFLAPLHCTALGKVLLAFGRLPLPESLERYTD
                     STLVTQFALKKNLASVREQGYAIDDEEFDIGVRCIAAPIFDFRNKVVGSIGISGPTTR
                     MTPGVLQTLVQQVLEISDKLNGRMKFNQGREQ"
     gene            27266..29152
                     /locus_tag="P5516_04465"
     CDS             27266..29152
                     /locus_tag="P5516_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014795.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRT91724.1"
                     /translation="MKVYSVEELLATRVKRANLISSAGGLRQALASASLDRKTDLTLS
                     EALVIGLIEQGVRTYYSVLGHGSTEVGEVLRVYQNAGLVRVFGLRSEIEASHAAAALR
                     WVKGEKAAVVTSIGPGAMQALAASLVPASNGIGVWYIFGDETTEDEGYNMQQIPRHQQ
                     AGFLKLTSVMGAAYSLHTPLALQSALLAGNVVVNHPYRPGPFFLLLPMNTQAALMENF
                     NLDELGSPAPVRLNAAVGAYDEAANWIKTSKRVLVKVGGGGARAGDELVKFLELSGGV
                     LVHTPIATGCVPYSHPANMSVGGSKGSICGNFAMENADLLVAVGTRAVCQSDSSRTGY
                     PNVKKVININADLEDALHYNHTLPLVGDVRLTLTKLNQMLEQENTPIDKAGWLKVCAE
                     KKKRWEEIKAARLAAPTLFDDYWQQEVLTQPAVLQTAIAWAKARGAVRFFDAGDVQAN
                     GFQLTEDEVPGQTFTDTGASYMGFAVSALLASGTASHPFYAVAFTGDGSFTMSPQILV
                     DGVEHGAQGCILLLDNGRMGAISGLQLDQYHHEVATANHILVDYLQWAKGVPGVLALD
                     GGNSLDELQAALNQAGNHPGLSLIRVKVYFGASELGGMGVYGRWNVGNWCAKTQQLRH
                     EIGL"
     gene            29161..30009
                     /locus_tag="P5516_04470"
     CDS             29161..30009
                     /locus_tag="P5516_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRT91725.1"
                     /translation="MTELSYEQITSLEKIARSLRADSLRFIYGRGSGHPGGALSAAEI
                     LAVLYFHELRIDPQHPDWSERDRFILSKGHASAVLYAALARRGFFDKNELKNWGEVDC
                     PHQGHPDRLKTPGVDMTSGILGHGVAVGVGLALAARMKKSPSRTYVLIGDGESQAGIV
                     WEGAAAAAKYRLSNLVVILDLNDVQLDGPMHEIMPMDHMAQRWEACGWYVMQVNGHSV
                     RQLVEGLDLAREIHDKPVCIIARTTKGKGVSFMENDSFWHGNVPSKEQLEQALLDLGV
                     EEEKNG"
     gene            30002..30961
                     /locus_tag="P5516_04475"
     CDS             30002..30961
                     /locus_tag="P5516_04475"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRT91726.1"
                     /translation="MAELSMREGYGRALADYGKLNPRVVAVDVDTSASTLSHFFAREH
                     PERFFNVGIAEPCAVDVGVGLALGGHIPFVNAFAALLALRSLEQIRTCVCYANTNVKL
                     AASYAGLSDFKDGPTHFAMTDIANMRALPNMNIIVPCDAAQAAAFVPLVAELVGPVYL
                     RINRGSTLPVYQPGSALQIGKGILHRQGIDLTIVAAGSMVGRALLAAQELDKDGIHAR
                     VVEIHTIKPLDGEILLDAAKETGAIVTAEEHVRAGGLGGAVSELLAETYPIPIERVGI
                     RDEFCPTGRNIEGLLDACGLSVAEIIFAARKVKKMKPGGAEKQ"
     gene            30958..31596
                     /locus_tag="P5516_04480"
     CDS             30958..31596
                     /locus_tag="P5516_04480"
                     /EC_number="5.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF006753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RpiB/LacA/LacB family sugar-phosphate isomerase"
                     /protein_id="HRT91727.1"
                     /translation="MKIAVINEISAAAKNAEIIHALTGRGHELLNLGNTGAADEPQLQ
                     YIHTGLMSALLLHLKVVDFVVGGCGTGIGYLNSVLQYPGVVCGLIHSPLDAWLFARIN
                     DGNCASLPLNQGYGWAGEINLRMIFDELFTPKRGSGFPEHRAEPQRNSRFTLAEISQT
                     THQPFATIIRDLPLSVTGPALQYPEFSQVLRESQQRDPMLEEAIHQILESGG"
     gene            31598..32968
                     /locus_tag="P5516_04485"
     CDS             31598..32968
                     /locus_tag="P5516_04485"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT91728.1"
                     /translation="MSVSAAPQKLKAKTKWLYGIGDIGNALVNSSLQFFLMKFYTDAA
                     LILPVLAGNALLVGKIWDAINDPLFGWLTDRTNSKIGKRRVFMLFGAIPLALSVALLW
                     FVPPGLSQVMTFVWIAGTFIFFDSVWTLTNVPYYALTSELTDDYDERSSLTVYRMVLA
                     TPAYLIGAALTPMIVGFFAVQRTGFAVIGIVYGVIAALALLISAGGLRERPNVIRSEG
                     EVNPIKSLKEAFRNRPFIYLFVVYFIVNISFAFAKTLMAYFIEYQLMMKDAISIALGL
                     MLICVTISLPIWKWVSTKMDKGPAYAIGMGMGAVALLVLWFLPAKPTVLIYPIMVLAG
                     FGFGANWIFPWAMIADVADYDRVKSGKFRSGVYYGVWGLATKISEALALAAVGWVLTG
                     FGYVPNIAQTPHALLGIRLFFSIVPAVGIFVGLPLLFKYPLTRKSHAEIKAKLLAMDA
                     AAKNDA"
     gene            32986..34065
                     /locus_tag="P5516_04490"
     CDS             32986..34065
                     /locus_tag="P5516_04490"
                     /inference="COORDINATES: protein motif:HMM:NF014893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HRT91729.1"
                     /translation="MKPIRVGIVGCGRISDLHQLGYRGRDDAVIAAVCDNNAAHAKKK
                     AKAWGVQKVYTDYQHLLEDKEIDAVELLTPHHLHCPMTVQAARAGKHISVQKPMALSA
                     AEADQMIDAANKAGVILRVYETFVFYAPVVRAKEMIDNGEIGEVRAVRMHVSTGTGDT
                     GWKVPTSALLWRIDEKKCGGGQLVFDHGYHLFSVGYYLGGPVEKVFAWIDKTPVTETG
                     GIVKVDAPAMIQFQYKAPRCYGNLDVEHTPKMRIYSDYYADDDRIEVIGEKGILFINR
                     YTARTVDLPELMIFKDGKTNPIPVEGVEWHDSFIATTHNFIDNLKTGGQPQLDGPTGK
                     AVLQFTLAALQSAATGKVVRPDDVL"
     gene            34081..34749
                     /locus_tag="P5516_04495"
     CDS             34081..34749
                     /locus_tag="P5516_04495"
                     /inference="COORDINATES: protein motif:HMM:NF019559.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysoplasmalogenase"
                     /protein_id="HRT91730.1"
                     /translation="MTYSLIPVPIHLVLIVLYMIARNRNDLKKTAIIQPSITFLAIVI
                     AGLSYLSPYVNPAYTTWILVGLVLCFIGDIFNIDMTNDKILFAAMVIFFIAYVEYAVA
                     FTRFGGFHTQDWLIGALMLVVYILLIRLYWKGLGNFRIPVMIFVLVISFMVTRAISTL
                     FSSQFSALSAWLVAFGGPLLFMGDVEYGLHRFHKPLKFHLGPLYYAGGQLLVALSCAY
                     LLFR"
     gene            complement(35028..>35229)
                     /locus_tag="P5516_04500"
     CDS             complement(35028..>35229)
                     /locus_tag="P5516_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91731.1"
                     /translation="LLDQNKGEEILENGYRRQEYYDYGYTDTDIELWGMDEPGAPDPF
                     FGGFVIADMADGKLDGNFDIIN"
BASE COUNT         7924 a         9543 c         9432 g         8130 t
ORIGIN      
        1 attttaagaa gatagcgcaa gaatttccac gaaaattctg gtcggtggtg ggagtctctt
       61 tcatcgatcg tgtgggtggg acgatgcttt tcccattttt cgcgctttat attacgcaaa
      121 aattccatgt gggcatgacc caggctggcg tggtgctggg gttgttttct gtctttggtt
      181 tggtcgggca gatgatcgga ggggcgctga ccgataaatt cggtcgccgc aagttgatca
      241 tcttcggttt ggtcttcagc gcgatcagca cattggcctt cgggttcatc aatacctttt
      301 ctgctctcat cccgttggcg ataggcgtgg ggctgctatc tgacattgcc ggcccggcgc
      361 acgcagccat gatcgcggac atcctgcccg aaaatcaacg ccaggaaggt tttggtattc
      421 tgcgcgtggt cggtaacctc gcctggattt tcggacccac cattggcggt tttgtcgccg
      481 gtaaatcctt ccttgctctc tttattatcg acgcggtcat cagcagcctg gtggcgttgc
      541 tgttctacct gctcattcct gaaaccaaac cgcagaatca gccgggcgag aaaccagaaa
      601 gcttgctggt cacttttaaa ggatatttcc aggtgctgcg tcatggggct ttcatcgcct
      661 ttatggctgc cggcatgctg atggggctgg tttaccagca gatgtatcat tcgctctcgg
      721 tttatctccg cgacaaccat ggcatagatc ctaaagggta tggatttttg ctcaccgcca
      781 gtgccatcgt ggttatcctc ttccagttca gcatgacgcg ctggattaaa aagaaacccg
      841 cctttaagat gatggccttg gggaccctgt tttacatgtt ggggttcagt atgttcggtt
      901 tcgtcggtca gtactggttg ttcgttctgg caatcatgat cattaccacc ggagagatga
      961 tcgtggtacc caccagccag gcgctggcag tgaacttcgc tacggaagat atgcgcgggc
     1021 gctatatggg tgtattcgga attacctgga tgctccctgc cactatcggt ccgggggctg
     1081 ccgggctcat ccttgatcat ttcaacccga acctgctgtg gtacatcggc ggagtgttgt
     1141 gcgccatttc agcgctggct tactacggat tgcacctgaa gattggcgcg caagagcgct
     1201 ttcagccggc tcaggatgaa cccgcggcgg tagaagaccc cgcggtgtaa tacccggcnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntc tttgagcgct tgcaatctgc
     1381 ccatcaacgc cggtcattcg ggtgcaattt cccagaccga aatcgcgctg aatgtggccc
     1441 tgacgcaatt cgctgccagc cagaccgccg cggtgccccc gacggctgaa cctacagcca
     1501 ccgttgccat ccctactgcc gcgccagaag tgctatcccc acccaacacg cctgaaccag
     1561 ccttcacccc cacgcgagga ggggtttggc tgaccgtaca ggaaaacacc aactgccgtt
     1621 ctggcccagg cagcctgttt gactttatgg ggttgatcaa cgccggtcag caggttgaag
     1681 cggttgctcg cagcacggtg ggcgagtact actacattaa gaaccctacc agctacagcg
     1741 attattgctg gctgtggacc cgttacacca ccgttgcggg tgacacgggt accctgccgg
     1801 tgttcacccc ccaacccagc cccaccccta aggtcacccc cacctttacc aaagcgccag
     1861 ctgatttcct tgtgaaatac ctgaacaccc agtcttgttc tggtttgtac gggattcagc
     1921 tctacattga aaacaccggc agcaccacct ggcaatccat cagagtgaga ttggatgaca
     1981 ctaccaacgc ggtgaccaca aaccataaga ataattactt cttcggtact gatgggtgcg
     2041 tgaaagacct gtccaatcgg caaggggatc tgaccccagg cgagtattcc cgtgtcgccc
     2101 ttgtcgatga cggtgaatta tcctataatc cttcaggtca cagtttcacc gctcaggtga
     2161 ccctttatag cggtgacgac caaacgggag tgtcggtaac caaaaccatc acttttaccc
     2221 cataaacgaa agtatctcag tactgaaaaa ggaaaccaag atgatcaaac gcatttttct
     2281 aacaataaca ttgcttttca ttgccgcgtt caccctcagc gcttgccagg gcggacccac
     2341 gcctgtggcg ccgcttgagc agagcgacct tcagaccctc gtggccggta ccttgaccgc
     2401 cgcggcagtg gaaatgcagc aaaccatgac cgccgcggta actccaaccg aaacgatcgc
     2461 gccaaccctg acggaaaccg ccactctgcc gcccaccgat acccccacca aggaggtcgt
     2521 gtccatcacc ctcagcggca acacgaactg ccgcagaggg gcttccactt acttccctgt
     2581 cattaccgtt atccctgccg ggtctgtggt agaagtgatc gcgcgcaacc cggagagcga
     2641 ttacttcttt gtgcgcgcgg ctgaatcaga tacgggaggc tgctggatct ggaaggaatt
     2701 cgcttcggtc actggcaacc tggaatcgct ggccgtctac accccggtgc cgacaccatt
     2761 gcctaccgcc accaacacgc cgccgcctgg agcgaatttc actgcccagt ttgtggggct
     2821 caccgcttgc ggtacgggtt acgctgctaa ttttaatatc agcaataccg gcacgagcac
     2881 gttgcaatcc atccgtataa aaatcacctc tacgagttgg aatttcacct tcatagataa
     2941 tactggtttc tcccagtgga gccacggatc caagtacgcc acgctggctg agataaccag
     3001 cgggaaatcg atgatcgtat caacctgtcc cggaggattt aattccagcc cctccggcca
     3061 ggacatcaaa gctgaaatca ccgtgtgctt aaacgataac ctgacgggnn nnnnnnnnnn
     3121 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3181 nnnnnnnnnn nnnnnnnnnn nnnnnnnncg aaactgggtt tgataaagcg tcgcgtaaag
     3241 ccccttttga gccagcaggc tggcgtggtt gccctgctcc accacccgcc cgcggtcgat
     3301 gaccaggatc agatctgccg ccaggatggt gctcaggcgg tgagcgatga cgatgctcgt
     3361 cctttgacgc atgacgcgct tcaacgcctc ttgaatgagc gcttccgctt cgctgtccag
     3421 gctgctggtg gcttcgtcca gaaccaggat acgcgggtct ttaaggatga cccgcgcgat
     3481 ggacaggcgt tgtttttcgc cgccgctgag ccggtatccg cgctctccca ccacggtatc
     3541 gtacccatca ggcagttcgc gcacaaaatc atgaatattg gccgccatac aggcttcttc
     3601 cagttcagcc tgcgaggcat ccggcctgga gtaaagcagg ttggtgcgga tggtgtcatg
     3661 aaagagatag gtttcctggg tcaccatgcc aacctggcgg gtgagcgatt ccagggttac
     3721 atcgcgcaga tcggtgccat ccagggtgat gcgcccgctg gaggggtcat acagccgggg
     3781 gatcaggtag gtgagcgtgg ttttccccgc gccgcttggg cccaccagcg ccaccaactg
     3841 cccgggtttc acgtgaaagc tgacgttttc aagagcgtta cccctggctt gagaacgccg
     3901 ttcttcaggc tcaagttcag actctccgtc actcagggtg gtcacgatgc tgtccatgct
     3961 gccatagcgg tgcacatcgc tcaggatgac gccatcgccc gcctggtagg cgaaggtgat
     4021 gttttcaaag gcgatctctc cctccacttc tgtgagcgct atggcatcgg gcttttcgct
     4081 gatttcaacg ggctgatcga gcacctcaaa cacccgttca aaactgacga aggaagaagc
     4141 aaaatccacc ggcgcgttaa ccagagactg caccgaaccg tacaggctca gcatgaaaga
     4201 gctgaacgcc acaattgtgc cgacggtgaa aaccccgctg ataacgaaat aaccgcctaa
     4261 tccatacacc agcgccgtgc ccaccgccac cagcagaccg atgatcgcgc caaagatcga
     4321 tcccgtttgg gcgcgattga tgccgtattc ttttacctgg ttggcacggc ggctaaagcg
     4381 ctctacctct tgcgcctgcc tgccaaagat cttcaccagc agcgtccctc caatattgag
     4441 ggtctcgttc atcatcgcgt tcatcgaagc gttggcttcc aactgtttgc gcacgatgct
     4501 ccgcaaccgt ctgctcaaaa gtgcggcaac aaccgcaaag aaaggcgcta ccagcatgct
     4561 gatcaaggtc aggcgccaat ccagcgtgaa catcaccacc agcaccgcga tggattgcac
     4621 caggctggtc acaattgaaa tcacactgtt ggtgaccgcg ttctgcgcgc caaccacatc
     4681 gttgttcagc cggctcatca actcaccaac cttgttgttg gtaaagaagc gcaacgacat
     4741 gcgctgcatg tgagcgtaga gcgagacccg taaatcacgg ataacgccct cgccgaccga
     4801 ggcgtttaaa cggcggttta ccacgctgat cgcgccgttg agcaaagcaa ccagcagcag
     4861 cgttaccgcc agtaagacaa gccgcctgac gttgccttgc gggatagtcc tatcgatcat
     4921 ttctcgtaag attaacgggt ttagcaggtt aagccccgag ctcgcgctga ttaacaacag
     4981 gatgactaaa agcgggagtc gatacgggcg ggcgtaggca aacacccgtt tgaccaaagc
     5041 ccaggtcata ttaggtagtt tttcttcagt ggccgcgagc cttctaatac catgcatcat
     5101 ttcgtttacc ctgctgttgt atacgtcaat gttaggttaa ataaatggat tggtcaagtt
     5161 tttcgcccat ctctttcgcc cgtttactgg ggagcgatgg tggcagtgca cgtggttcaa
     5221 ccgcagcggc aacccaactg gcgccggcgg taccacaagt tacccgtgcg atagtcaccc
     5281 aacatgctgg atacatcacg ccgcgcatgc ttgatggtca aatgggtgct tggcatggaa
     5341 gaaagtgcca cgaatgataa gtgaatgata ggttatcaaa tggaaaaaga gcaaaccatc
     5401 ggtataatgg aaacattatg cagagcaaac ccaaactggc gattttcgat tttgacggga
     5461 cgctcgctga tacacggccg tttatgttga gcatcctcga tcacctggct gaacgcttta
     5521 acaccaggcg agtggatatc cactcgcttg aacagatgcg gggttatact gccaggcagt
     5581 taatggaatt gtatcgcgtt cccatgtgga aatttatttt gatggcccgt gagtctcaaa
     5641 gattgctgta tcagaacatc acctccatcc agctttttcc gggggtagaa agcgcactgc
     5701 gcgccattgc ccggcgaaat gttgccattg cggtggtcac ctcaaaccag ctgcgtaacg
     5761 tccttgcggt gcttggcgaa gaactcgccg ggttggtttc agtgtttgaa tgtgaggcag
     5821 gcttcttcac caaggcgcgc aaactgcgca gcgcgctgcg tcaagcaggc gtgaaacctg
     5881 gcgaagcggt cagttttgga gacgagtccc gggatatcga agcggcgcgc aaggttggta
     5941 tcccctgcgc cgcggttacc tggggctatg cccatagcga agttttacgc catcatcaac
     6001 ctgattacct gttgaccagt gtcgatcaga tcatgggcat tgtggctgaa taataacagg
     6061 caggtaaaag agcgtgcgca ttgctttaag aacctgaacg attgagaatt ttgccgcgtt
     6121 tcttcacaaa aaggtcacgc gttccttaat gtcgctcttg ctttgtgagc caaattgggt
     6181 ttttgcgcgc gtattcacgg cgggagcaaa aatcaactac aatggggtac atcatggaag
     6241 cccttcatta cctcctttcc gggctcggtg ctttcgctgc aggcgcggtc aacgctcttg
     6301 ctggcggcgg cacgctgatc acctttcctt tattaaccgc cttggggttg ccagcggtga
     6361 gcgctaatat caccagcacg gtatcccttt tcccagggta cttcggagcc acctgggcac
     6421 aacggcgtga cctgggcggg cagcgcgcca ggttgatcgc gcttctgcct gctgccgcgt
     6481 taggaggatt aattggcggg ctgctgctgt tttacagtgg cgaaaagacc ttcaaacagt
     6541 tgattccctg gttaatcctc ttttcctccc tgctgctggc gttgggtgaa ccgctgcgtc
     6601 aaatgcttaa gcggtcgcca cacccgcatc cgcagcgccg ctctccacgc ctgtggctca
     6661 tttcactgcc ggtctgcctg gcagcgatct atggcggtta ttttggcgct ggtttgagcg
     6721 tgattctcct ggccattctg gcgctttttt tggatgactc tctgacccga ctgaatgggc
     6781 ttaaacaatt tctcgctttc gccgccaata gtggcgcggc cattttcttc ctgttttcgg
     6841 gcagggttaa ctggcgtgtg gctggggtga tggcgatcag cgcgctgttg ggcggtattg
     6901 ccggagggaa actggctggt cgggtgagtc ccgtggtact gcgttgggtg gttgtgagca
     6961 tcggggttgt tgtggcagtc atcttcttta tccaaattta tcttaaacct tgaaaggcgg
     7021 tacttttatg ttgaatacga aaccgcgcct agcgctgatc attgcctgca gtatgtacct
     7081 ggcatttggt atgttcaacg ctgctatcgg accggtactg ggtgaactgg ctgaaaacac
     7141 ccattctaca ctggtaatgg tagggggtgt catcacgttt ttgttcctcg gctcgctcgc
     7201 cacacagctt atcgccgggc caatcatcga taagtttggc ctgcggattg tggggctggt
     7261 atccctgttg ctggtcgcgt tcgggctgcc cgcgtttacc aatgtccact cttacccgct
     7321 gatgctggcg ctggttttat tgaccggtct gggtcagggg gggatggatt tgaccgccaa
     7381 cctgctggtt gcagacgcct accccagtaa atccacgcct atgttaaacc tgctgcattt
     7441 cttcttcggc ttaggcgctt tcttcgggcc cgcgctggtg gggctggcta ttgttgcaac
     7501 cggttcaggc atgatcatcc attgggtggc agcggggatg ttcctgctgc tggccctcat
     7561 cctgctgcgt gttcccgcag gcaaagcccc agccccggat gctacaaaaa aacctgatgc
     7621 aaaagtgaag gacgagaaga gcatttacct ttctcccttc ttgtgtacca tcggggtgct
     7681 gattctcatt tacgtcggca cggaatatgg tttaggcagt tggtcgaccc gttacatggg
     7741 tctcactacc ggcatggctg atcagaatgg agcgctggta acctccgctt actggggcgc
     7801 gctgaccctt gggcgccttg ccggcgccgc ggccagccgc cggctcagca acacgcgcct
     7861 gctcggtgtt tcacttggtg gcagtttact cggcaccttg ggtttggttc tcagccgcgg
     7921 ttccgttctg agcaccgttg ttttcatcgt catcaccggt ttctcgctcg gcacggttta
     7981 tcccacatct gtagcggtca ccgtggagaa gttcccgaaa gaccagggga aaactgtggg
     8041 gctgttggct gccatgggca gcattggcgg cctcgccctc ccctggctgg cagggttctt
     8101 acttgaaacc acttcagcct tggttttcgc cctcttctta tgcgccacca cagttgccat
     8161 gttgctgctg ctttttgtgc tgggtcaata caaaaagggg acatcgaacg ggtaaaacag
     8221 tgaacaagag gaaatgaagt acgctcttgc cactgtgagg gagaaggcaa gggctttacg
     8281 cggatttatg ctttcgtctc gatatgccga acgaccagcc tgacgccttc gaccttttca
     8341 acgatcactt tctccccttt ttttaggggg ggttctccgg gtgccaacac cgcgctccac
     8401 tgttcacccg ccacctgtac gatcccgatc ggagccaggc gtgtaactac atagccatct
     8461 tttccttcga gcgattcccg accggtaatc acaggtttct tcatcgcccg cagggcgatc
     8521 atcactaccg cgaagaaaga aagccccagg aatactccca ttccgatgac caacccgacc
     8581 gaaaccctgg gaaatcccgg tatggaaagc gaattaaaca ggatcagcgc gccggcgatg
     8641 aacgaacccg taccagccac ggtgagtgcg ccgtgcgtcg gcgctttgat atccaggatg
     8701 aacagaacaa aagcaataac caagaaaatc atgccaaacc agttcaccgg caggatacct
     8761 aacccataaa tcgccagcgc cagcagcacg gccccaataa accccgccac ccagccaccg
     8821 gggctggaaa tctcaatcag gatggcctgc accccaatcg aaagcagtaa aaaaacgata
     8881 ttggggtttg tgagcaaacc caacacatct tctaccatgg tcgtgctcac tggtacctga
     8941 agcgcgcctt tcgagcggat gaccacctcg ttgccattca tcagcacctt gctgccgtcc
     9001 atttgggcca gcaggtcctc cacgtcgtgg gcgatgaaat cgatcaaccc tgcctgcagc
     9061 gcttccgttg agttggccgc tctggccttt tcaatggatt cttccgccag cttcaccgcc
     9121 tcagtcccgc gccgggctgc cagtgagcgc gcgctggctt taagtatttc cttggtcttt
     9181 tcttccaggg tggatccgat atcttcaccc tgcataccta ccgggctggc tgcgccgatg
     9241 gtcgattcag gcgccatggc ggcgaaatgc cctgagagca ccaacagcgt gcccgcggat
     9301 gccgccatcg ccccgcgtgg ggtaacatac accaccaccg gcaccgggct ggcgaggatc
     9361 tgctgcacca ggtggttcat caaatcgacg ctgccaccag gggtgttcaa ttcaataatc
     9421 agtaacccgg ctccagtgct caccgcccgg tttatcccgc gctgcagata accgctccaa
     9481 accggggtga gcggtccttt aagttgcacc acaaccgctt ctggcgcgtc cacctgcccg
     9541 ctgaccagct gaagcggttg aaagaagctg accaaaatgg acagaatgag actgacctgt
     9601 aaaaaccatc gcgttttcat gaagtcgcgc tcctttaaag attatagcac gcgcccaccc
     9661 ctgctgtgcc tctccaatag tcctcctatg aaatgttgat actttattaa taatcacctg
     9721 ctattctaga cctgtatgaa aagtatctgg ttacaaggag aaatacaatg ggaaaaataa
     9781 ttggtatcga tctgggaaca accaactcag tggtagccgt tatgcagggt ggagacgccc
     9841 aggtcatcaa caccgctgaa ggcgcgcgcc tgtgcccttc tgtggttgct tttaataaaa
     9901 atggcgagcg ccttgtgggg caaaccgcca aacgacaggc agtgatcaac ccggaaaaca
     9961 cagtgttctc gattaaacgc tttatgggac gccgattcga tgaggtggaa agcgaacgca
    10021 agatggttcc atttgaggtg atctccggtc cgatgaaaga cgcgcgtgtg cgcatccctg
    10081 tgacaggccg cgattactca cctcaagaga tttctgccat gatcctgtcc aagctcaaag
    10141 cagacgccga agcctacctg ggggagaagg tcagccaggc ggttatcacc gttccggcgt
    10201 attttaatga cagtcagcgc caggccacca aagatgccgg taagattgcc gggctggaag
    10261 tgctgcgcat catcaatgag cctaccgctt cagccctggc ctacgggctg gataaaaaga
    10321 agaacgaaaa gatcctggtc tttgacctgg gcgggggtac gtttgatgta tccgtgctgg
    10381 aggtaggtga aggtgttttc gaagtcaaat ccaccagcgg tgacacccac ctgggtggcg
    10441 acgactggga cgaggttatc gtcaactggg cggctgatca gttccaggcg gaccagggaa
    10501 ttgatctgcg taaagatcgc caggctctgc agagattgcg agaggctgcc gaaaaagcca
    10561 agatcgagct ctccacagta atggaaaccg agatcaacct gccctatatc accgctgacg
    10621 caaacggtcc caaacacctg gtcaccaaac tcagccgttc tcgcttcgag caaatgaccg
    10681 cgcatctgct ggatcgctgc aaaaaaccgt ttgagagcgc gctgaaagac gccgggctta
    10741 aagcatctga cattaacgaa atggtgctgg tgggcgggtc cacgcgtatg ccgcaagtgg
    10801 tggatatggt gcgagggctg atcaacaagg aacctaacaa gggcgtcaac ccggatgaag
    10861 tggtcgccat cggcgctgcc attcagggcg gcgtgctcgg cggcgaggtg aaagacatct
    10921 tgcttctcga cgtgactccg ctctcgctgg gcgtggaaac aatgggcgcg gtgatgacac
    10981 ccatcattga gcgcaacacc accatcccca tccgcaagtc tcaaatcttc tcaacagcgg
    11041 aagatgggca gacggcagtg gatattcacg tactgcaggg tgaaagaccc atggcggcgg
    11101 ataatatgac cctcgggcgc ttccggcttg aggggatccc caccgctccg cgcggtatcc
    11161 cgcaaattga agtgaccttt gatatcgatg ctaacggtat cctcaacgtc accgcgcagg
    11221 ataaagccac gggtcgtgag cagaaagtga ccatcaccgc ctccaccaac ctgcagaagt
    11281 cagatatcga gcgcatggtg gaagaagcca aagcgcacaa atctgaagat gaccgccgta
    11341 aagagctggc cgaagctcgc aacattgccg acaacgccgc ctaccaggtc gaaaaggcgg
    11401 tcaaggaagc cggcgaaaag gtaacagcca gcgatcgatc gtccatcgaa gcccgggttc
    11461 aggaactgcg cagcgcgatg accggagagg atgtttcgcg catcaagagc ctgacagaaa
    11521 ccctgcagca ggattacgcc cgcgtgatgc aggcttctgc ccaggcgcag accgcgcccg
    11581 gcggtggtga tgccggcggc agcgcgccac ctgacgacgg cggcgacgtg gtggacggcg
    11641 aattcaccga aaagaattaa cctgtttaac agaatcaagc caaaaggcgg ccagttggcc
    11701 gccttttgct tcgcgccagg tgccattgac caaccagccc atcccccagc gcaacccaac
    11761 aacgctgcgg gctaacgcgc agtatataca tgttagaatt cagccattct tattattctc
    11821 aggccaacat gagcgcttct gtcccctcaa cctttactcc tacccccatc gtccgagtcg
    11881 cggagcgcct gctcaccgcc ttcctcaagg tggccgcgaa gcagtatctt tctcttcaac
    11941 caaccaacct gtcctctgtc ccgccgccgc agcccggaca tcgttacact ttatacgccc
    12001 acgtgccttt ctgcgaaagc ctctgtcctt actgctcttt caaccgcttt gtgttagacc
    12061 gggagaaagc cgcggcttac ttcaaatctt tgcgcgcaga aatgcgcctg gtcgccaacc
    12121 tgggctaccg tttttcttcg ctttacctag gcggcggcac ccccactatc gtcccagatg
    12181 aactggcgcg caccattgac ctggcgcggg agctcttcag tatcaacgag gtcagctgcg
    12241 aaaccaaccc caaccacttg aacccggaga tggtcacgct attgaaagag cgtgtacaac
    12301 gcctttcagt tggcgttcag agtttcaatg acgggctgct caagcaaatg aaccgctatc
    12361 aaaaattcgg ttccggcgcc agcatcctcg aaagcatccg ccgtaccgct ccacacttca
    12421 aatccctcaa tgtggatatg attttcaact tccccagcca gacgccgcaa attttgcgcg
    12481 atgatctgca cgcggtcatc gactcaggcg cgcagcaggt gaccttttac ccgctgatgt
    12541 cttcaccctc ggtcaggcgt tccatggcca gcacagtcgg tgaactcatc cctgcccgcg
    12601 aagccgatta ttacgccatc atcagccagg gattagcgca ggatttctcc gcgctctccg
    12661 cctggacttt cgcgcgtaat ggcagcaacc tgattgacga gtatattgtg gataacgaag
    12721 aatacgtggg ggtgggatcc ggcgtatttt catacctgaa tggctgcctt tacgccagta
    12781 cattttcctt acaggaatac agcgacctgg tttctcaagg ccggatggcg ctcagcgggg
    12841 cacagccttt cagccggcgc cagcaaatgc gttacaggat gatgatggac atgttcggcc
    12901 tgcgcttcga tcgcgccgcc ttcaggcagc gctttggtgt tcctgccagc cgcgggctat
    12961 ggctggaaat gctcttcttc cgcctgctgg gagcgtttga gaagaagaat cctgccatcc
    13021 ctactcccgt tgggcgctac ctctcgctgg tgatcatgcg cgaattcttc accggtgtca
    13081 accaggtgcg tgatatcgcg cgcgcagccc tttccccgta cgaccgcggg gaatgttacg
    13141 cgccctgaaa gttgagaaac cccgccggaa agccgcagcg agcgcacagg cttacccagc
    13201 gcctgccgcc agcctttgac gcgccgggct tcaccatcgc cgagcacgcc aaagggaggt
    13261 tctatctttc caatccattc aataaagact ggaaagaagg ttgaccattg atggtgctgg
    13321 cgcatttttt cctgcctccc tcgggttcac agttaaccag caagaagcac aacaaacggc
    13381 aagacccgcc cagcaatcag tacgccgaaa gcaacagagt gttgctcgat taaccatcgt
    13441 tgagacaatc atctgactgt ttgactgtgt gttcgccctg ttcctggtat aagccctggc
    13501 cgtaataact gaagagagag tgcatatcca gatgttataa tgctcgcatg cgttacaggc
    13561 gaaaatggct actccttata ctggttgtgt taatcgtcgc cggtctaacc ctcagccttc
    13621 ctcccgtttg gtcgcgcatc acctatcata cccacaacct gttcgcggac attaaatatc
    13681 gtctcaatcc tcctcaggct gaggtttttg ttccttctgg cgctgaagaa ggaaatttga
    13741 ttgcgactgc ttccgcagca acactgacgg cgctcgttcc cacacccact tcacccattc
    13801 tgacaactga agaaactcgt ctcccctctt ccacgcctac tctcacacct gtacccctgc
    13861 caatcaaagt ctacctgcct ggagttaaac ctgaacccca gttatggaac aactgcggac
    13921 cagcaaccct ttcaatgaat ctctcctttt atcaatgggg caaaactcag gtggaggctg
    13981 cggaggtgct caaacccaat ccgcgcgata aaaacgtgat gccttacgag atggtcactt
    14041 ttatcaacac acagacctcg ctgcgcgcgc tttcacgtat ggggggcgat gtggagaccc
    14101 tcaaacgcct gctcaacgcg ggcttcccgg tgttggtgga aaaaggtttt gagcccggcg
    14161 atctgaaaaa agaaggctgg atggggcatt ttaatctggt cattggttat aacgatgatg
    14221 ctcagtcctt cattacccaa gattcttacc tgctcagcca ccccccgggg ggaggcgaga
    14281 tcaaagacct cgaaaccttc ccgggtttca ctgtgccata cgaagatatg caaaaggact
    14341 ggcgcgcgtt caattacgtt ctgattgttg tttacccgcc cgaaaaagag aatgatgtgc
    14401 tcaatgccct tggtccgctg atggacgaga atggtgctta tcggttggcc tatgaacgcg
    14461 caaaaatgga agcgaccaca ctgagcgata tacgcgaacg ctttttcgcc tggttcaacg
    14521 ccggcaccag cctggtgtac ctggcggatt acaatggtgc ggctgcggct tatgattccg
    14581 ctttcaacct ttacgcgcag ataccagaga acgcccgccc ctggcgtatg atgtggtacc
    14641 agaccggccc ttacttcgcc tattactatt ccgcccgcta cacagatgtg atcaacctgg
    14701 ctgaccagac gctgaaacgc atgagcgcgg aacctatcct tgaagaaagt tactactggc
    14761 gcggcatggc ttacctggcg ctgggagata acgagcgcgc gcgcgccgag tttcgcgaca
    14821 gtcttaagta tcacccgggg tttggcccca gcctggtcgc gcttgaacaa atgggggaaa
    14881 ctccctgaga ttttcggcta tacttgtaat ggttagaaaa tttcatgatc aagatcctgc
    14941 attttgctga cgctcatatt gatattgcca cacacggaaa acatgaccct gaaaccggct
    15001 tgcccatccg cgccctcgac tttttaaaag ccctggatac catcattgac cacgccattt
    15061 ccgaaaaagt ggatctggtc atattcgccg gagacgccta caaagaccga accccatcgc
    15121 ccactttcca acgtgaatgg ggaaaacgca tcatccgcct gccacaggcg gatatccccg
    15181 cacttttact ggtagggaat cacgatatca gtcccgccag cggacgcgcg cacaccttgc
    15241 aggaattcga caccctgcag gtgccgaaag tgcgggtgct ttacaaaccc gagctgctta
    15301 aacccgcgga cctgtggggg ctgcagttgc aggtgctctc gctgccatgg gtgttcagct
    15361 ccagcctggt ggccgcgctg gggctttccg ctgtagaaga agacactgtt cacgaggaga
    15421 tcgaaaaacg cctcaccacc atcctgcagg cgcggatgga cgaacttgac cccgcgctgc
    15481 ccactgtgct ggtggggcac ggctccgtgc agggggcggt ttatggcaat gaacgcaccg
    15541 tcatgctggg caaagacctg gttctgcctg gctcactggt gaaagaccct cgcctggatt
    15601 acaccgcgct cggtcatatt cacaaatacc aggacctcaa ccctgggctg cacccgccag
    15661 tcgtatacgc aggttccatt gaacgggttg atttcggcga ggtcgcagat gacaagggct
    15721 ttgtcatcgc tgaggtggag aaaggaaaca cctcgtaccg gtttgtaaaa ttgcacggtc
    15781 gcgccttctt tgaccggctg gtcaggcttt ccatggaaga cagggcgatg gaaaaaatcc
    15841 tcgcttcctc accgaacgaa gctgaagccg agaacgccat cttcagactg gtcattgagt
    15901 atccgcgtga gcttgaagcc gatattgatg agcctgcctt acgccaaaaa ttcgcctccg
    15961 cgctcgaatt tcacctggtc cgccgcccgc aagcagaggc ccggctgcgc ctgcccgctg
    16021 atcagaacat cgccagctac agtccgttgg aattgctctc gacttactgg aaatccactc
    16081 acgcccaacc tcgtgatctt gcagggctgc aaacacttgc tcaatcgatc tttcaatccg
    16141 taaccggatc aaagcccggg taaggacggc aaggttatcc gacatgtcca atacattgat
    16201 tgaaatcctg gtcattttcg tcctgatctt gtttaacggt tttttttcta tgtcggaaat
    16261 tgccattgtt tcagcccgca aagtgcgcct ggaacaacgc gcggatgaag gagatgcggg
    16321 cgccggtcgc gcgctgcagc tctccaagga gaccgggcaa tttttgtcct ccgtgcaggt
    16381 gggcatcacc ctggtggcga tgctcacggg cgcttttggc ggcgccactc tggcagagcg
    16441 gctgacccag tcgctcgccc gcgttccctg gttggccacg tcggccgcca cgctctccct
    16501 ggtcattgtc gtgctggtca ccacttattt ctcgttggtc attggcgagc ttattcccaa
    16561 gaagatcgcg ctcaacaacc ctgaaaaaat cgcttcaaaa gtctccggta tcatggtgtt
    16621 tttctcccgg ctcacatcac cgctcacgcg cctgctttct tcctccactg acctgggcat
    16681 gcgcctgctg ggcatcaagc cctccggcga accccccatt accgaagaag agatcaaagt
    16741 actcatcgac cagggaacac aggtgggggt atttggtgaa gcagaacagg acatggtgga
    16801 aggcattttc cgtttgaacg accgttcaat caatgccatt atgaccccgc gcacggatgt
    16861 cacctggttg gacgcggacg acagtatcga agaaatactg aagaccatcc tccaatctcc
    16921 ctattcccgc tttccagttt ccagggagtc attggacaat gtgctgggaa ttctcaaatc
    16981 cagagacttg cttgaaaagc agctttcagg cgaacctttt aacctgttgg acctgctgca
    17041 aaagccactt ttcatcccag aaaacacgcc cgtcatgcgc gttcttgaaa tggagcgtga
    17101 gcacggggta catgaagccc tggtgatcga tgagtacggt ggcgtgctcg gattcgtcac
    17161 cctttttgac gtgctcgaag ccattatcgg tgaactcccc agccaggacc aggcttcaga
    17221 acccgaaatc gtgatgcgcg aagatggttc ttacctgatt gatggtttac tgcccattga
    17281 cgagtttaag gacctgttcg atctggacct gctccctgaa gaagataaag tgggattcca
    17341 aaccgctggc ggattcgtga tgaaccaggt gggttccatt ccccacgccg gtcagacttt
    17401 ccaatggaat gatctgctat ttgaggtgct ggatatggac ggcctgcgta tcgacaagat
    17461 cttggtcagc cgcaatagcg aaaaaacgcc gcctgctgga ggggataaat gatcatcgac
    17521 cgcctgcaaa tcagcgggtt tctttcatat aaagagaaag tggatatcga cttctcaggt
    17581 ttcgccctgg cctgcatttc aggtcaaaac ggcgccggta aatccagtct gcttgaagcc
    17641 atcacctggg cgctcttcgg tgaagcgcgc cgtaaagacg acgccatcat ccacatgggt
    17701 actgataccg ccgaggtgtt gcttgagttc gattacgagt ccacgcgtta ccgggtacag
    17761 cgcagcaaaa ctcgcgacaa gagcacacag cttgagtttc aactgcgcaa cgctgaaggc
    17821 aactgggtgc cgctcactga agccaccatg cgcggcactg aagagctcat ccgccgcacc
    17881 cttcacctgg actatgaaac atttaccaat gcctcgtttt tcctgcaggg caaagcagac
    17941 cagttcgctc agcagcgccc gggtgaccgt aagcgtatcc tatcaggcat cctcggtttg
    18001 gacatttggg aggactaccg cgaggaagca gcgcggcgcc gtaagctttt ggaaaatgac
    18061 cttgccctgg ttatcagcca tttacaggaa attgagactg aactgcaaca ggaagcagaa
    18121 cgaaaggaac gtctcgcccg cctggaaaag gaagtgagca cccagacgga actctttgag
    18181 gcgcgaaaag cctatcttga gcagcagcgc caggttgccg agcgtattct cgccgaccaa
    18241 aaacaggtag aaaaacaacg cgcggagatt aatcgcttgc gcgcagagga tgaaacgatg
    18301 cgcagcgatc tcgacctgcg cacgcaagaa cgtcagaccc atctttccct gttacagcag
    18361 gaaaaagaga tcaggcagga ggtggacgcc tgggaacagg cgcgtaaaac cctcgaagag
    18421 tgggacacgc tggcgcagga ttttcaccag tacgattccc agcgtcaggc cccgctgcag
    18481 gccatcgcgg tagaagaaag ccgcctccag gccgagttaa actcgcttca acagaaacag
    18541 gctgaaatct tctcaataga acaggaactg cctgagatcc agcggctggt tgaagactcc
    18601 agcaaagcag tcgaagactg cagcgcaaag ctggcaaccc gagcacaact ggaaaaagaa
    18661 atgcgcgctc tcctggatga aatcgcccgc gcccgggctg aaaacacgcg cctaaaaatg
    18721 gagatggatg aaattgttga gcatcgcact cacctggaga atatcagcgg cgctaactgc
    18781 cccacctgtg aaaaacccct ctccgacgat gagcgccaaa ggctggtgga tgagatcaac
    18841 gcgcgcggca aagaaaaggg cgacgcgcat cgcgaaaata aaaggataat cgaacaaagc
    18901 gaagcccaat atcgtgagat cgaaaccgcg ctcctcaacc tgcagcgggt ggacgccgag
    18961 ttgaaactgc aacaacgcat cttcgacacg aaagcgcagg aattacgcat gaaggaagcc
    19021 gcgttcactg actggcagca aaaaggcgcc gtgcgcatgt tggagctgga agagacgttg
    19081 cgctctcaat cctttgcgct ggaagcgcgc aagcagttag ccatgatgga tgctcaattg
    19141 aagaaactgg gttacgacgc cgccgctcac gaacaggcgc gccgtatgga actggatggc
    19201 cgcgccagcc aggacaaaaa aatacagctg gagaaggcca ggtccgccct cgcccccctc
    19261 gaacaaaata tcacctcgtt gcaaaagaac atggagaaca accaaaagcg cctttcagcg
    19321 ctgcttgatg agctcaggca ggacgaggaa aaactggaga tcgaaacttc caacctgccg
    19381 gatctcgatc aattggaaag agattactac gcagcccagg agcagatgaa ccaggcgaac
    19441 agggatttag ggtacacgcg caatcaggtg gaagtgctgg aaaagcagcg cgaactaaag
    19501 gtggagaaac tcgaagagaa agaccagctt aacaaccagg tcactgatct caaaacgctg
    19561 gaacgcgctt ttggcaaaga cggcattccc gcgctgctca tcgaacaggc gctgccagaa
    19621 attgagcgtc acgcgaacga gatcctcgat cggctaagct cagggagtat gtctatcagc
    19681 ttcgaaaccc agcgtgattt caaagataag aagcgcgaag aacgtcgtga gacgctggac
    19741 attctcatcc gcgaccccgc cggtgaacgc ccctacgagc tcttctctgg tggcgaagcc
    19801 tttcgggtca attttgccat ccgcctggca ctcagccgtg tgctggcaca tcgcgccggg
    19861 gcgcgcctgc agaccctcgt gattgatgaa ggttttggca gccaggacgc tgacgggcgt
    19921 caacgcctgg tagaagccat caacctggta agcacggaat ttgagaagat cctggtcatc
    19981 acccacctcg aagaattgaa agacgccttc ccggcgcgta ttgaagtgac caaaacgccg
    20041 gctggttcta aggttgaggt aatcgctgta tgaacatgat tcacgcccgt gtcgggttgc
    20101 agcagcgtgt gctgccggag taccgcgcgg aattcttcga cacgctggct accgcctgcc
    20161 tgcaggggtt gggggttttc gcgggtgagc cctgccaggg tgaagccgta gaagttgttg
    20221 accagctctc tattgcctac tacacacacg cacgtaacct gcattttttt cgcggcagcg
    20281 cctattttta ctggcaggca ggggttttag gctggctgca gcgctggcag cctgacgtgt
    20341 taataatgga aatcaacccg cgtaatcgtt ccaacccgct ggcggcgcgc tggatgaaag
    20401 aacgcggacg cccggtgctt ggctgggggt tgggtttacc cggcgccgga aagatctacc
    20461 ggcttatcta ccatagcctt ttacgttcct gcgacgctgt gatcgcctac tccaccaccg
    20521 ccgccctcca gtaccgtcat gccggagtgg acccatcccg cgtatttgtc gccgctaacg
    20581 cggtcacccg ccgaccaacg gacctggtcg ttgcacgacc gccgtccttc catgaaaaca
    20641 ccccgcagct cctcttcgtt gggcgcctgc agccgcgcaa acgcgttgat acgctgctgt
    20701 acgctttggc catgttgccc cctcaccaac aaccgcggct atccattatt ggagacggac
    20761 ctgaccgctc acgcctggag atgctcgcct ggcaggtcta tccacgagcc gtgttccatg
    20821 gtgccaaacg cggtcaggaa ctggaccctt attaccaggc cgctgatcta ttcatccttc
    20881 cgggaaccgg gggactggcc attcaacagg ctatggcgca tgccttaccc gtcatcgtag
    20941 gcgcggccga cggcacccaa attgaactgg tgcgtgagga aaacggctgg acgctgccgg
    21001 atgacagccc ggaaaccctc gcccgtgtta ttcgagaagc gctgtcagat gttgagcgcc
    21061 tgcgtcaaaa agggctggca tctcgtcgta ttgtttctga agaggtcaac ctggaaggca
    21121 tggtaaagac cttttgcaca gcggttgaat ccgttctctc aaaggcttag aggaggaaag
    21181 cgtgaacctg ttgatcattg cagatgggcg cagcccaatt acccggcgct ggatacgcat
    21241 gctgcagccg ctgaattaca ctatcaacct ggtctctacc tacccctgtc aaccggtcga
    21301 cggagtgaac gaaaccatcc tcttcccggt ggcgttcgcc ggactatccg gcagccaggc
    21361 gggaggcggc gccaggggcg ggcgaaaagt agtttcgcgt taccgtggac tcgctgccac
    21421 cctgcgccac tggttgggtc cattgaccat cacccatgcg gagaaagatt atctcgagct
    21481 gctgaaccgc ctgcagccag acctcgtgca tgcgctgcgc atcccttatg aaggcatgct
    21541 tgccagccgc accccaacgg gcattccgct ggtcatctcc acctggggca acgactttac
    21601 ctatcatgcg atctcaaatg gggtgatggg cagcgccact cgccgcgcgc tgctgcgcgc
    21661 ggatgggctg atttctgata ccgtccgcga cctgcgcatc gcgcgcgcct ggtccttcaa
    21721 ccccgccaag ccggctgtat ctgtaccggg taacggaggg atcgacctgg ctgagatcga
    21781 gaacatcacc caggggatcg acccagctgt gccctggcaa gtgatcaacc cgcgcgggtt
    21841 ccgcagcggc tccgtgcgca acgacacttt ttttaaatcc atcccgctca ttcttagcct
    21901 gtacccagaa gtgcagttca tttgtccatg gatggcggga caacccgaag ccctgagctg
    21961 ggtgaatcgt ctcaggatac tggataacgt ttccttgtta ccgatgatct ctcaggctga
    22021 attatggcgc gagttcgctc gctctctggt atccgtctcg gtcagtgagc acgacggcac
    22081 gcccaactcg ctgcttgagg ccatagccat cggctgcctg cccgtgtgcg gggatatcga
    22141 atcaatccgc gagtggatcc gcaatggcga gaatggtatc ctggtcgatc ccgctgatgc
    22201 ttacgcgctg gccgaggctg tgcagcgcgg aataatggat gaagaattcc gccgcgctgc
    22261 cgctgctcgc aaccgtgaga tcatccgtga gcgtgccgaa attaccatcg tgaggcaaaa
    22321 ggttgccgag ttttaccaac gcatgaccgg gttcgtgtaa taaaaacagg ctggcatttt
    22381 gccagcctgt tttttgtctc gctaaaggtt gtggatgacc ccctcttttt tatacctcat
    22441 aaataactgc ccccagccgg tgttttggca ccattcctta aagacatatt tttcttttaa
    22501 tggccagtgc acataatcat gatacacctc tgaaaaggcg atggggatgg ccaccagcgg
    22561 tgtacgggta accagctttt gcagaaactt ggtcggacca taccaggtaa gccagcccat
    22621 gaactgatgc agattgtgac ccactttgaa gtgccagttc tctcctgaaa tatcatcccc
    22681 caccaactcg atctcacgag gatcgcccac tcccaacccc tggttgtgac caaggttgat
    22741 gtacttgatg ctcatcgggt caaaacccat cagtttggct gccactgcat caatggccac
    22801 ctggtcttgt gatgccaaaa tgacattctt cacttccggc ttcatcaccc gcggaccggt
    22861 gccattgccc gcggtggtgc cgtccatcac cgcgaacaat cctgtgtgga tttctttctg
    22921 gatagccagt aggtcgatga tggtctcgtg gatatatgaa tgggtgtaat gtcgatgctt
    22981 ggtgagcaga cctccaaatg cattcttaat ggcgccggtg gtggtggtgt agatatggca
    23041 tttcgccgtc ggcagatgca cgatgttctt tcctacaaag taatctggaa tgtagatacc
    23101 ttccggatag atatggtcga gggcattcat cttcgccttg ggcttgtact taacccaggt
    23161 catatctttg tcttcaaagt tatacagcac cgggatatgg tagtgtttga agatgggaac
    23221 gtaaccgttg aggtcttctc ctttgaaagc gtttgtcacc acggtctggt tttgcacaca
    23281 ggtaatatca ttgtagcctt tacttttcag cgccaggatg gtgccttcca tctgccaggg
    23341 tgtggtgttg gcgctgggca tcggaaagtg ccaagagata ttatctttga ggatggtagt
    23401 agcaccaggg tccagcgctt taggcccatc agccagttcg aaaagacgaa tgtagtcttc
    23461 gataacggtt tctggcgtag tcttgagtac agcgactttt gaattcattc tccacgtccc
    23521 ttcacacgtc cttttatggg tgaattatac caacaggcag ttcgcctggc caacccagcc
    23581 tgtgtacgcg caaccccggc tgatttggtc tataatcctt ctgagttcat gaacaagaag
    23641 atgcctttga aacaacccgg ctggaatttc cgcagtcgcg cctgcggcct gttgatttgc
    23701 ggtctgttac tgttggcgtc ctgtaacttt ccaatacgcg acttttccgc tttgccccga
    23761 ccttacgcga ccttggatcc agcgatcttc gaataccatc gttctgccac acctactgcc
    23821 gggcagcctg gcgctgctcc cacccgcaca cctcagcctc cgcccgtggt ggatgaatca
    23881 ctgtaccata tttacaccat ccgttctggt gacacattcg aaacggttgc caggcacttc
    23941 agtgtcactc ccgctgctat catcagcatg gaagcgctgc cccaaaccgg aatcctccct
    24001 ccaggccgtt acctggtcat tcccaagaac accgacaacc ttttcgagca agagatgatt
    24061 ttgcctgaca gcgcggtgat caactcgcct tgcgccgcgc cttttgatat cagcgcctat
    24121 gtaagcgctg ccggcggcta cctgagcact ttcagccagc ccgtggcggg cattcgctca
    24181 agtggggcgg aaatcgtgcg ccgtgtcgct gataatcaat ccgtaaatcc aatgctgctg
    24241 ctggcaatcc ttgagcaccg tgcgggattg gtttttggaa aacaaccacc tgcagatatt
    24301 aatcaccctt tgggtttccg taacatgtac tacaaggggc tttaccagga gttgtcgctg
    24361 gcggctcgta tggtaaacac cgggtactac ggctggcgtt atgggacgat gaccatggtc
    24421 acttttactg accaggtttc gcagcggatc tccccgcact tgaacgcagg ctcagttgcc
    24481 ctcatgtatc ttttctcgca atcgtatccc tcaacagatt ggaacgagag attgcttgga
    24541 aaaagcggat ttatggaaac ctacctcgcc atgtttccag acccctggtt gtgtgccgca
    24601 gaagttgagc cgttgttaag tgacacgctc agcgcgccgc agttagagct tcccttcgcc
    24661 ccgggtgaag aatgggcatt taccgccggg ccgcattaca gctgggtgga gggcacccct
    24721 ttgggcgcgc ttgatttcgc gcccaatatt aaaagcgctg gctgccgggt ctcgccttcg
    24781 tgggtgcgcg cttcagcccc aggggtgatc acccgggcgg ataacggcgt ggtgatattg
    24841 acccttgagg atgaaaaggg tgacgtcacc ggctgggaag tgatgtatct gcacatcgcc
    24901 gcaaacgagc gcgttccatt gggaacacgc ctgcgaaaag acgaccccat tggtcatcca
    24961 tcctgtgaag gcggctcttc cacgggaaca catgtgcata tcgcccgcaa gtacaagggc
    25021 gagtggattg gcgcgcaagg actgttccca tttgtattaa gcggctgggt ggcttacccc
    25081 ggggaacgcg cctaccaggg ccggttgatg aaagacgggc aggtggtcat cgcccgccag
    25141 gggggcaacc aggactctct catcaaacgt tagtattcca ggtgtggatg gttggttggg
    25201 gaaattaaaa taaaaaagga tggtttcgcg tctccgacca tcctttcaaa taaccaatgc
    25261 gactgtttaa tcggcttcgc gcatgccaag ctggtgatca agctggtaca gacctcccag
    25321 gctgccttcc agtttcgtca ccatcgcgag aacagaggaa gcattctttt cttcttcgac
    25381 ttgttcattg atgaaccact gtaagaagat ctggctggcc acatcattgg cttcaactgc
    25441 cttggcgtaa atgccattaa tcagcccggt gactttctgc tcgtgctcat acaccttggt
    25501 aaatacatct ttcacgcctt taaattccac tggtggttgt tcaatggctt tcaggctgat
    25561 cttctcgcca cggtccagca ggtagtcgta gagcttcatg gcgtgctcct gctcctcttc
    25621 tgcctgtact ttcagccatt tggcgaaacc tggcaagttt tcggtttcgc aataagccga
    25681 cattgagaga tagaaatagg cagaatacag ctcgtgttta atctggtcgt tcatcgcttc
    25741 atatactttt gcattcataa gacaccctct ctgacgaaga attattattt ttacctgtta
    25801 agaataacac aactctgttt gaaatttgtt aattttttat gaccctgtaa cgtttgagtt
    25861 tttaagtatt cggcgaaaac ctatatatgt gcgcctttca agaccgctta aacgcgattg
    25921 ctttaggcgc agtgttggca gaaaacctct gcatgctgct ggagttcctc tcgacgcgtg
    25981 ctttacaaga cctcgaaacc gagatgatag acaagacact gccagcgggt gcttctccgc
    26041 gcctgggtgt ggcagtatgc caatccgcat aaaatggatg gtaagcaggc tgattggata
    26101 atccgctaga aaaaaaaatg gtctcttctg ctatctttaa gtttaaagaa gggtgggctc
    26161 aacaggggtt aagacccagt cctactcacc gtacaggacc gacgcctgaa tcgatctggt
    26221 ttccatgaga tctctgtatt ggaggccctt ttttgtcgag taaggactat ctcctgtctc
    26281 tcatcgttcc ctggctgttt tctgaccagc gctcttctag tgatgctgat ttggcctgct
    26341 ttgtctgtct ttgagagccc tttcactggt ggcttgccag ctttaagcac tcacttgcac
    26401 cccccttttt cggatattcg tgatggtgac ttttgtacga atagttgtgt aatacgcaat
    26461 taagttgtat tatatgcaaa tatcctatac actctaattg ttcaaggtga aaaatgactg
    26521 agatccaatc gcttgcccgc ggattgcgga ttctgttgat gctttctgat tcgctcgatg
    26581 ggagcactgt gacagaagtg gcggagaacc ttggggttga caagggaagc gcttcgcgcc
    26641 tggtaaacac actggtcaaa tacggcttcg cggaaaaaga cccggtttcc cggcggattt
    26701 ccctggggcc gcagattgtt tctttgagcc gtagcttact taccaggatg ccgttgcgcg
    26761 aggtggcaaa accttacctg cgtgagctga tgcgctccac cggtgaatgc gcccatattg
    26821 gtatcctttc ccagggcaaa gttttatata ttgaccaggt ggaatcgccg gcttctttac
    26881 gggtaaacgc ggaggtaggg tttcttgccc cgctgcattg caccgcgctc ggcaaggtac
    26941 tgcttgcttt tgggcgccta cccctccctg aatcccttga acgttacacc gactcaacac
    27001 tggtgactca atttgcgctg aagaaaaacc tggcgagtgt gagggaacaa ggctacgcga
    27061 tcgatgatga agaatttgat attggtgtgc gttgcattgc tgcgcccatc ttcgatttca
    27121 ggaataaggt ggttggcagt atcggcatat caggaccaac cacccgcatg actccaggcg
    27181 tattgcaaac cctcgtgcaa caggtgcttg agattagcga caaactcaac ggcaggatga
    27241 aattcaatca ggggagagaa cagtaatgaa ggtttattca gtagaagaac tgttagccac
    27301 cagggtcaag cgcgcgaatc ttatttcatc ggcaggaggg ctgcggcagg cgttggcctc
    27361 tgcgagcctt gatcggaaaa ccgacctgac cctctcagaa gccctggtta ttggattgat
    27421 cgagcaagga gtacgcacgt attattcagt tttaggtcac ggttcgactg aggtaggtga
    27481 agttctgcgg gtataccaga acgcaggatt ggtgcgtgtt tttgggctgc gcagtgagat
    27541 cgaagcatca cacgcggcgg cagcgctgcg ttgggtaaaa ggggagaaag ccgcggtggt
    27601 tacctctatc gggccaggag cgatgcaggc actagctgct tcgctggtgc cggcatcgaa
    27661 tggcatcggg gtttggtata tcttcggcga tgaaacaacg gaggatgaag ggtacaacat
    27721 gcagcagatc ccccgtcacc agcaggcagg gtttttaaaa ctaacctctg tgatgggcgc
    27781 tgcctacagc ctgcacacac cgctggcctt gcagtctgct ttgctggctg gcaatgtggt
    27841 cgttaatcac ccctatcgac ctggtccatt ctttttattg ttgcccatga acactcaggc
    27901 agcgctcatg gaaaacttca atctggatga acttggttca cctgctccgg tacgattgaa
    27961 cgcggctgta ggagcgtatg acgaagcggc gaattggatc aaaacttcaa aacgagtgtt
    28021 ggtcaaggtg gggggcgggg gagccagggc tggagatgag ttggtgaagt ttcttgaatt
    28081 atcaggcggg gtgctggtgc acacacccat agccaccggt tgcgttccct actcgcaccc
    28141 ggcgaatatg agcgtgggcg gatcgaaagg ctcgatttgc ggcaatttcg ccatggagaa
    28201 tgctgatctg ctggtcgctg tgggcacgcg ggcggtctgc cagtcagatt cttcaaggac
    28261 cggttacccg aacgtaaaaa aggtgatcaa tattaacgca gacctggagg atgccctgca
    28321 ttacaaccac accctgcctt tggtgggaga tgtgcgcctg acattgacga agctaaacca
    28381 aatgctcgaa caggaaaata ctccaatcga taaggcggga tggctgaaag tttgcgcgga
    28441 aaagaaaaag cgctgggagg agataaaagc agccaggttg gcggctccaa cgcttttcga
    28501 tgactactgg cagcaggagg tccttactca gccagccgtc ctgcagaccg cgattgcttg
    28561 ggcaaaagcg cgcggcgcgg tgcggttctt tgacgcgggg gatgtgcagg cgaacgggtt
    28621 tcaattgacc gaagacgaag ttcctgggca aacatttaca gatacaggag ccagttacat
    28681 gggcttcgcg gtttctgccc tgctggcgtc cggaacggca tcccacccat tttacgccgt
    28741 ggcgtttacg ggagacggct cgtttaccat gagcccgcaa attttggttg atggtgttga
    28801 gcacggcgcg caaggctgca tccttttact ggataatgga aggatggggg cgatttcagg
    28861 gttacagctt gaccagtatc accatgaagt agccaccgct aatcacatcc tggtagatta
    28921 cctgcagtgg gccaaggggg ttccaggcgt gctcgctctt gatggtggaa attcactgga
    28981 tgaattacag gccgcgttga accaggccgg aaatcaccct gggttatccc ttattcgggt
    29041 aaaagtttac tttggagcaa gtgaactggg tggtatgggt gtgtatggtc gctggaacgt
    29101 agggaactgg tgcgcgaaaa cacaacaatt acgccatgaa attggccttt aggagaggtt
    29161 atgacagaac tatcctatga acagattaca tcactggaaa aaatagcgcg gtctttgcgc
    29221 gctgacagcc tgcggttcat ctacgggcgc ggttcgggcc atcctggcgg ggcgttatcg
    29281 gcagcggaga tactggctgt cttatatttc catgaattac gaatcgatcc ccaacaccca
    29341 gattggtcgg aaagagacag gttcatactg agcaaagggc acgcttcagc ggtgctctac
    29401 gcagcgctgg ccaggcgcgg tttctttgat aaaaacgaac tgaaaaactg gggagaagtg
    29461 gattgcccgc accaggggca cccggaccgt ttaaaaaccc ccggagtaga tatgaccagc
    29521 ggcatcctcg ggcatggagt ggcggttggc gttgggctgg cgttggcggc gcgcatgaag
    29581 aaatctcctt cgcgaacata tgtgctcatc ggagacggtg aatctcaggc gggaatcgta
    29641 tgggaaggag cggccgcagc ggccaagtac cgcctgtcca acctggtggt tatcctggac
    29701 ctcaatgacg tgcagctcga tggtccaatg catgaaatta tgcccatgga ccatatggcg
    29761 caaaggtggg aagcctgcgg ctggtatgtg atgcaggtaa atggtcacag cgtgcgccaa
    29821 ctggtggaag gtttggatct tgctcgagag atacatgaca aacctgtatg catcatcgcc
    29881 cgtacgacca aaggaaaagg ggtgtccttt atggaaaacg attccttctg gcatggcaac
    29941 gtaccgagca aagaacaact tgagcaagcg ctgcttgacc ttggcgttga ggaggagaaa
    30001 aatggctgag ctctccatgc gtgaaggata cggccgcgca ttggcggatt atggaaaact
    30061 gaaccccagg gtcgtggcag tggatgtaga tacttccgct tccacgctct ctcacttttt
    30121 tgcccgtgaa cacccggaac gtttttttaa tgtgggaatt gctgaacctt gcgcggtgga
    30181 tgtaggggta ggtctggcgc ttggcgggca tatccccttt gtgaacgcgt tcgctgcttt
    30241 gctggcattg cgctcacttg aacagatccg cacctgtgtt tgctacgcca ataccaatgt
    30301 aaaactggca gccagctatg ccggtttatc cgatttcaaa gatggcccga ctcactttgc
    30361 catgacagac atcgccaaca tgcgggcact gccgaatatg aacatcatcg ttccctgcga
    30421 cgctgcccag gctgccgctt ttgttcccct ggtggcggag ttagtgggac cggtttacct
    30481 gcgcattaat cgtgggagca ccttgccggt ttaccaaccc gggtcagcgc ttcaaatagg
    30541 caaaggcatc ttacaccggc agggcatcga tctgacaatc gtcgccgcgg gttccatggt
    30601 ggggcgcgct ctcctcgccg ctcaggagct cgacaaggat ggaattcatg ccagggtggt
    30661 cgaaattcac accatcaaac cgctggatgg cgagatcctg ctcgatgcgg ctaaggagac
    30721 cggcgcgatc gtaaccgctg aagagcatgt acgtgccggt ggattaggcg gcgctgtctc
    30781 cgagcttctt gcggaaactt acccaattcc cattgagcgt gtgggaattc gcgacgagtt
    30841 ttgcccgacg gggagaaata tcgagggatt gctggatgcc tgcgggctat ctgtggcgga
    30901 gattatattt gcagcgcgga aggtaaagaa aatgaaacca ggaggggccg aaaaacaatg
    30961 aaaatcgcgg ttattaatga aattagcgcg gcagcaaaga atgcggaaat catacacgcg
    31021 ctcacagggc ggggtcacga gttattgaac ctggggaata caggcgcagc ggacgagccg
    31081 cagcttcaat atattcacac cggtctgatg tctgcgcttt tattgcacct gaaggtggtg
    31141 gatttcgttg tgggcggatg tggtacgggg attggttatc tcaactcggt attgcaatac
    31201 ccgggagtcg tttgcggcct tatccattct ccgttggacg cctggttgtt tgcccgtata
    31261 aatgatggta attgcgcttc tctcccgtta aaccagggtt atggttgggc gggagagata
    31321 aacctgcgca tgatttttga tgaattgttc accccaaaaa gggggagcgg gttccctgaa
    31381 caccgtgctg aaccccagcg caactcccgc ttcacattgg cggaaatttc acagacaacc
    31441 catcagccat ttgcaacgat catccgtgac ttacctttgt cggtgaccgg gccggcacta
    31501 caataccctg aattttctca ggttttacgg gagtcccagc aaagggaccc tatgttggaa
    31561 gaagcaatcc atcaaatact tgaatcagga ggataaaatg tcagtctcag cagcgccaca
    31621 gaaattaaaa gcgaagacca agtggttgta cggaattggg gatatcggga acgcgctggt
    31681 taacagctcg ctccagtttt tcctgatgaa attctacacg gatgcagcgc tcattttgcc
    31741 tgtgctggca gggaatgcgc tgctggtagg caagatctgg gacgccatca acgatccgct
    31801 ttttggctgg cttaccgaca ggacaaattc caagatcgga aaacggcgcg tatttatgct
    31861 cttcggtgca attccgttgg cattgtcggt ggctttgctc tggtttgttc cgcccggtct
    31921 cagccaggtg atgaccttcg tttggatcgc tgggacattc atcttctttg attcagtctg
    31981 gacgctgacg aacgtaccat attacgcgtt gacctcggag ctgacggatg attacgacga
    32041 acgcagcagc ctgacggttt accgcatggt actggctacc ccagcatatt tgattggcgc
    32101 cgcgctcact cccatgatcg tcggtttttt tgctgtgcag cgcaccgggt tcgcggtaat
    32161 cggcattgtt tatggtgtga tcgctgccct ggcactgctc atttccgcgg gagggttgcg
    32221 agaacgcccc aatgtcattc gctctgaagg agaagtcaac ccgatcaaat ccctcaaaga
    32281 agcgtttcga aatcgcccct tcatttacct gtttgtggtc tatttcattg tgaacatctc
    32341 ttttgccttt gcaaaaacgc ttatggctta ctttatcgag taccagttga tgatgaaaga
    32401 cgccatatcg attgctttgg gcttgatgct catctgtgta accatctccc ttcccatatg
    32461 gaaatgggtg tctacaaaaa tggacaaagg acccgcgtac gctattggca tgggaatggg
    32521 cgcggtggcg ctgctggtgc tgtggttcct tccagcaaaa cccacggtgt taatttaccc
    32581 gatcatggtt ctggcggggt tcggctttgg cgcgaactgg atcttcccct gggccatgat
    32641 tgcggatgtc gcggattacg accgggtaaa atcgggcaag tttagaagcg gcgtgtatta
    32701 cggcgtttgg gggctggcaa cgaaaatatc agaagcgctt gctcttgccg ctgttggttg
    32761 ggtattgacc ggctttgggt acgtacccaa tattgcccaa accccgcacg cgctgctggg
    32821 aatccgcctg ttctttagca ttgtgccggc ggtaggaatc tttgtggggt taccactact
    32881 gttcaaatat ccccttacgc gtaaaagcca tgctgaaatt aaagcaaagc tgttagccat
    32941 ggacgccgcg gcgaaaaacg acgcataacc aagaaggatg atgacatgaa accgatcagg
    33001 gttggcattg ttggctgtgg caggatctct gacctgcacc aattgggtta ccgcggacgg
    33061 gacgacgcgg ttatcgccgc ggtatgcgac aataacgctg cgcacgccaa aaagaaggcc
    33121 aaagcctggg gtgttcagaa agtttacacg gattaccaac atctgctgga agacaaggag
    33181 atagatgcgg tggaactgct cacaccgcat cacctccact gcccaatgac cgtgcaggca
    33241 gccagagcgg gtaagcatat atccgtgcag aaacctatgg cactctcggc agcggaagct
    33301 gaccagatga tcgacgcggc aaataaagcc ggggtgattt tacgggtata tgaaactttc
    33361 gtattttacg ccccggtagt ccgcgcgaaa gaaatgattg acaacggaga gattggcgag
    33421 gtgagagcgg ttcgcatgca cgtcagcacc ggcacaggcg atacaggctg gaaagtgccg
    33481 acttccgcct tgttatggcg cattgacgaa aagaagtgtg gcgggggtca actggtgttt
    33541 gaccatggct accatctgtt ttcggtgggc tattacctgg gaggaccggt ggaaaaagta
    33601 tttgcctgga tcgataaaac gccggttacg gagacgggcg ggattgtcaa ggtggatgcg
    33661 cctgctatga tccagttcca atacaaggct ccacgttgtt atggcaacct ggatgtggag
    33721 cacacaccaa aaatgcgcat ttattcagat tattacgctg atgatgaccg tatcgaggtg
    33781 attggagaaa aaggaatttt gtttattaac cgctacaccg cgcgcactgt ggacctgccg
    33841 gaactgatga tcttcaagga tgggaagacc aatcccatcc ctgtggaagg ggtggaatgg
    33901 catgacagtt ttattgccac cacacataat ttcatcgaca atctgaagac cggtggacag
    33961 ccccagctgg atggaccgac cggaaaagcg gtgctgcaat ttaccctggc tgcgctgcaa
    34021 tccgcggcga ccggcaaagt tgtacggcct gatgatgtgt tgtaaagagg aaggatatat
    34081 atgacatatt cactgattcc agtccccatt cacctggtgt taatcgtttt atacatgatt
    34141 gcccgcaatc gtaatgactt gaagaagaca gccattatcc aaccatctat caccttcctg
    34201 gctatcgtga tcgcagggtt gagctactta agcccgtatg tcaaccctgc ttacaccact
    34261 tggatattgg tcggcctggt tttgtgtttt attggtgata tttttaacat cgatatgacc
    34321 aatgataaga ttttattcgc cgctatggtc atcttcttta ttgcctatgt ggaatacgcg
    34381 gttgcgttca cgcgttttgg cggtttccat actcaggatt ggctcatcgg cgcgctgatg
    34441 ctcgttgttt acattcttct aatccggttg tattggaagg gactgggaaa tttccgtatc
    34501 ccggtgatga tcttcgtgct ggtcatcagc tttatggtca cgcgcgcgat ttccacgctt
    34561 ttcagcagtc aattttcagc gctttcagcc tggctggtcg cgtttggcgg tccgctgctt
    34621 tttatgggcg atgtagaata tggattgcat cgcttccata agcccttaaa attccatctc
    34681 gggccactct attacgccgg cggtcagtta ttggtcgcgc tcagttgcgc ttacttactc
    34741 tttcgctaaa ggtaacgagt tacgttaagg gtgaaaaccc gggtaaaatg aaaaactgaa
    34801 gcaatgttat tttgaacatg gtctaagaag gcttctgctc gcagctatgg tcgaataaat
    34861 atgaaaacgc atttcacctc ctggtattga atgaaaagcg atttaaaagg ctgcttacca
    34921 aaaacctcta attgtctggc aaacctcgca aattggccga aacgaatgaa agcggagatt
    34981 gtaggattta cgggaaatat gattgaaatt ctatagcgat tatttggtca attaataata
    35041 tcaaaattgc cgtccagctt gccatccgcc atatctgcga tgacaaaacc accgaaaaat
    35101 gggtctggtg caccgggttc gtccatgccc caaagttcga tatcagtgtc tgtatacccg
    35161 taatcgtaat actcctgccg tcggtatcca ttctccagaa tctcctcgcc cttattttgg
    35221 tccagtaaa
//
LOCUS       DAOTLW010000019        34531 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1500_length_34531_cov_9.834842, whole genome shotgun sequence.
ACCESSION   DAOTLW010000019 DAOTLW010000000
VERSION     DAOTLW010000019.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 34531)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 34531)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34531
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1500_length_34531_cov_9.834842"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            72..380
                     /gene="rpsJ"
                     /locus_tag="P5516_04505"
     CDS             72..380
                     /gene="rpsJ"
                     /locus_tag="P5516_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012084982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRT91732.1"
                     /translation="MAKQKIRIRLKAYDHRVLDQSAKRIVETAERSGAQVVGPVPLPT
                     RIERFTVRRSSFIDKDSHEHFEIRTHNRLIDVLDPDAKTIDMLMRLNMPAGVDIEIKI
                     "
     gene            642..1271
                     /gene="rplC"
                     /locus_tag="P5516_04510"
     CDS             642..1271
                     /gene="rplC"
                     /locus_tag="P5516_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRT91733.1"
                     /translation="MFKGLIGKKIGMTQIFDENGAAHPVTLIEAGPCFVTQVRTVQKE
                     GYSAVQLGYEEVKPRRLASGELGHLRRNSLPPLKYLREFRAKEVQVAEGDKVDVNLFA
                     VGEKVDVSGISKGKGFAGGVKRYHFRGGPKSHGQSDRMRAPGSRSSTTTPGRVFKGAR
                     GAGHMGNDAVTAQNLKVVLVDAERNVIGVNGSVPGARGGLVLIKESRKG"
     gene            1319..1957
                     /gene="rplD"
                     /locus_tag="P5516_04515"
     CDS             1319..1957
                     /gene="rplD"
                     /locus_tag="P5516_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRT91734.1"
                     /translation="MEVEVLNMEGKKVSKVELPPAIFEAPINIDLMHQAYVMQMANAR
                     LGTHETKVKSEVSGGGRKPWRQKGTGRARQGSTRSAQWKGGGKIHTPHMRSYVQNMPK
                     KMRQAALRSALSVKAAESSIVVVDALKLKEPKTRLMASALNTLVGDAKALVLIPSAES
                     YEDVLRSTNNIPDAKVLNANYINVRDIMVFDKLIMPVAALELITANLGGVGE"
     gene            1959..2261
                     /gene="rplW"
                     /locus_tag="P5516_04520"
     CDS             1959..2261
                     /gene="rplW"
                     /locus_tag="P5516_04520"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRT91735.1"
                     /translation="MSTIYDVLRRPIVTEKTNYMVTKLHQYVFEVADDANRTMVKDAV
                     EKLFNVNVLRVNIINAPAKRSRRARSRRLLIRNAEYKKAVVTLSPEDRIPIFEGVE"
     gene            2261..3097
                     /gene="rplB"
                     /locus_tag="P5516_04525"
     CDS             2261..3097
                     /gene="rplB"
                     /locus_tag="P5516_04525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRT91736.1"
                     /translation="MAIKVYKPTTPSRRAMTGYSFEEITKDTPERALIVIRKARAGRN
                     NHGKVTVRHQGGGSKQYIRIIDFKRDKREIPARVAAIEYDPNRTARLALLTYVDGEKR
                     YIIAPVGLKVNDTVVAGPMAEVRPGNSLPISNIPVGTLVHNIELKEGKGGQLVRSAGA
                     SAQLLAKEGDFAQIRMPSGEVRLVPEKCYATIGQVGNTDHSNIKLGKAGRKRHMGIKP
                     TVRGTAMSPRDHPHGGGEGRQPVGMSGPKSPWGKPTRGYRTRRNKKTDQYIVRRRNAG
                     NR"
     gene            3138..3416
                     /gene="rpsS"
                     /locus_tag="P5516_04530"
     CDS             3138..3416
                     /gene="rpsS"
                     /locus_tag="P5516_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRT91737.1"
                     /translation="MSRSLKKGPFVDPKLLKKIEAMNTRSEKKVVRTWSRASTIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHRLGEFAPTRHFRGHMSDKSTGAR"
     gene            3419..3760
                     /gene="rplV"
                     /locus_tag="P5516_04535"
     CDS             3419..3760
                     /gene="rplV"
                     /locus_tag="P5516_04535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HRT91738.1"
                     /translation="MATDIRAELKNCGVSAQKTRLVIDLIRGKPAVEALTTLKFTNKA
                     AARHVGRLLASAVANAEENFGVNRDDLVVYKISADDAPLRKWRRFGARGRFKPLLRRS
                     SHITVVLREKE"
     gene            3821..4498
                     /gene="rpsC"
                     /locus_tag="P5516_04540"
     CDS             3821..4498
                     /gene="rpsC"
                     /locus_tag="P5516_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRT91739.1"
                     /translation="MGRKVHPIGFRLGINQPWEGRWYAEGSDYRTQLHQDLVIRRMVQ
                     SQGEKAGISLVEIERFPGKVKVVVHTAKPGILIGRKGDSVKKIRTDLEALTGKAIDLE
                     IKEIKAPDCDAFLVAKNIAGQIERRVSYRRAMKRALQQAMRQGAEGIKVSVSGRLSGA
                     EMARIVWLREGRVPLQTLRADIDFARAEALTTYGRIGVKVWVYKGEVIPGVEEKVEPT
                     EGVYVSE"
     gene            4546..4974
                     /gene="rplP"
                     /locus_tag="P5516_04545"
     CDS             4546..4974
                     /gene="rplP"
                     /locus_tag="P5516_04545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRT91740.1"
                     /translation="MLMPKRVKWRKVMRGRMTGKAQRGADMIVGEYALQAVEPGWVTA
                     RQIEAARRTIVHSMKRHGKVWIRIFPDKPYTHRPPETRMGKGKGNVEYYVAVVKPGRI
                     MFEISGLEREEAIHALNQAGYKFSIKTKVLARGQEAGEGV"
     gene            4971..5183
                     /gene="rpmC"
                     /locus_tag="P5516_04550"
     CDS             4971..5183
                     /gene="rpmC"
                     /locus_tag="P5516_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002509269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRT91741.1"
                     /translation="MKTSEIRLLSTEEIKTKLADTRNELMNFRFQLVTGQLTDTSRLK
                     TTRRQIALYETILREREQGKKLEGAK"
     gene            5180..5458
                     /gene="rpsQ"
                     /locus_tag="P5516_04555"
     CDS             5180..5458
                     /gene="rpsQ"
                     /locus_tag="P5516_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRT91742.1"
                     /translation="MNQRRRLTGIVTSNRMMKTVVVEIARTYKHPLYHKVVHARQTIK
                     AHDELGCAIGDKVMVVECAPMSKEKTWMVQSILKREGKTLDVAEEAVE"
     gene            5455..5826
                     /gene="rplN"
                     /locus_tag="P5516_04560"
     CDS             5455..5826
                     /gene="rplN"
                     /locus_tag="P5516_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRT91743.1"
                     /translation="MIQQETRVKVADNTGARELLVIQVVGGSRRKYGAVGDVVIATVK
                     SAAPQGSVKKSEIVKAVIVRASKEWRREDGSFIRFDDNAAVILDVDGQNPKGTRIFGP
                     VARELRERGYMKIISLAPEVL"
     gene            5849..6181
                     /gene="rplX"
                     /locus_tag="P5516_04565"
     CDS             5849..6181
                     /gene="rplX"
                     /locus_tag="P5516_04565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRT91744.1"
                     /translation="MKVKIRKGDQVEVISGTINDKGKRGEVIKVLPDENRVVIQGVNT
                     RTKHQRQVQSQGRTVNPGLVRFEAPISISNVAIVCKKCNKSTRVGLVREGDKTKRQCK
                     ECGALIDE"
     gene            6212..6745
                     /gene="rplE"
                     /locus_tag="P5516_04570"
     CDS             6212..6745
                     /gene="rplE"
                     /locus_tag="P5516_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRT91745.1"
                     /translation="MKERYIKEVSPALMKSLGLSNVMQIPRIKKVVINIGMGEAMENP
                     KALDAAVQDLSAITGQKPVITKARKSIANFKLREGRAIGTAVTLRGEKMWAFLDRLMN
                     IVLPRVRDFRGVSAESFDGRGNYTLGLREQIIFPEIEYDKVDKVRGMEITIVTTAPSD
                     DQAAALLQLLGMPFRKE"
     gene            6747..6929
                     /locus_tag="P5516_04575"
     CDS             6747..6929
                     /locus_tag="P5516_04575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HRT91746.1"
                     /translation="MAKKCMEYRELRREYPNQVRNRCKRCGRPRGYLRRFGLCRICFR
                     ELALEGEIPGVTKSSW"
     gene            6951..7364
                     /gene="rpsH"
                     /locus_tag="P5516_04580"
     CDS             6951..7364
                     /gene="rpsH"
                     /locus_tag="P5516_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009754342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HRT91747.1"
                     /translation="MSVNDPIADMLTRIRNGVIGSQSVVAMPHSKVKAEIARILKEEG
                     FIEGYEVVEGDKPGMMTLRIKIKYVGERREKTPVITGLERISRPGRRVYSKKQDIPWV
                     LAGMGIAILSTPKGVMTGQRARQLGIGGEVLCKVW"
     gene            7392..7940
                     /gene="rplF"
                     /locus_tag="P5516_04585"
     CDS             7392..7940
                     /gene="rplF"
                     /locus_tag="P5516_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:YP_500964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRT91748.1"
                     /translation="MSRIGKLPIRIPDSVQVEINGSEVTVKGPKGEMKRQFSPDIIIT
                     QEDGMLLVKRNSDLAPVRALHGTTRALLFNMVTGVSTGFTTELEVDGVGYRVEMEGEK
                     LVLFVGYSHPFKIDPPKGVHFEIDSKTHLVKINGSDKEVIGQLANDIIKIRPPEPYLG
                     KGIRYKGQKIRRKAGKAGKAKA"
     gene            7956..8321
                     /gene="rplR"
                     /locus_tag="P5516_04590"
     CDS             7956..8321
                     /gene="rplR"
                     /locus_tag="P5516_04590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRT91749.1"
                     /translation="MAKNNRTEARIHRHRRVRKYISGTESRPRLSVFRSLAEIYAQVI
                     DDQAGVTLAAASSIDHELRGKTDGKKKLDQAKLVGELLARRAKDKGITRVVFDRGGFR
                     FSGRVKALADAAREAGLEF"
     gene            8361..8882
                     /gene="rpsE"
                     /locus_tag="P5516_04595"
     CDS             8361..8882
                     /gene="rpsE"
                     /locus_tag="P5516_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018376782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRT91750.1"
                     /translation="MDYQPQETQFDERVIEIARVAKVVKGGRRFQFRVTVVVGDNNGK
                     IGIGIGKANAVPDAMRKATERAHKNIRKVTLTGTTIPHEVTGRTAGAVVLLKPASAGT
                     GVIAAGGVRAVLEAVGVHDILTKSLGSANVLNVVKATFDALAQLKSPETEAARRGKPV
                     KELMPYWERRKNA"
     gene            8875..9084
                     /gene="rpmD"
                     /locus_tag="P5516_04600"
     CDS             8875..9084
                     /gene="rpmD"
                     /locus_tag="P5516_04600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HRT91751.1"
                     /translation="MPKKKEALKKVRITLVKSAIGYSETHKATIRALGLRRMNQTVEQ
                     VDSPVIRGMLMKVNHLVKIEEQEVK"
     gene            9081..9533
                     /gene="rplO"
                     /locus_tag="P5516_04605"
     CDS             9081..9533
                     /gene="rplO"
                     /locus_tag="P5516_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HRT91752.1"
                     /translation="MKLHDLMPNEGAKKAPKRKGHGISAGQGKTAGRGTKGLGARAGS
                     GGHLYRQGGNLPFYRRLPFIRGEGFTPPNRVEFNEVNLDQLERFQAGSIVSRETLQEN
                     GLLHKASNPVALLGRGEVKVALTVQVQRVSKSAREKIEAAGGKVEIVA"
     gene            9556..10887
                     /gene="secY"
                     /locus_tag="P5516_04610"
     CDS             9556..10887
                     /gene="secY"
                     /locus_tag="P5516_04610"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HRT91753.1"
                     /translation="MKRSAWRYLWTSEDIRKKLLITVLLLVIYRLAANIPVPGINREV
                     IRSISEMGGPGASLFNLLDLLSGGTISNFSILAMGVYPYITSQIIIQLLVPIIPALDR
                     RLKDNPREGRSLMEKWTTMLTIPMAFLSAIGQVNIFNSMAKTGSVLTTPFGFVGASLL
                     PTITLLLSMVAGTMFGIWLGQLISEFGIPNQGLSLIIFAGIISKIPSNLLGILADKEN
                     GWWVLIIILVLLALTIFAIVYVQQGRRNVPVLFPGRRIGNRMSMPVRSNLPLMVNMAG
                     MIPLIFAQTFLSFPALIASYFIAGKTSGFWYGVYSLFSGSGWFYPIIFFVLVVAFTFF
                     YTDVLFTQQNYGDTLKKQGAQIPGVVRGGPTQKYLTRVQRRITFPGAFFLGFVAVLPY
                     IIGTILPITRASAFVISAAGLLIVVGVVRDTFTIIETELKVHGYDESIIKG"
     gene            10889..11545
                     /locus_tag="P5516_04615"
     CDS             10889..11545
                     /locus_tag="P5516_04615"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HRT91754.1"
                     /translation="MAVYIVLLGPPGAGKGTQAQLIAQKYGLMHVSSGDLFRENLKNE
                     TELGKLAQTFMQRGELVPDDVTIAMVRERLGRPDCQKGALLDGFPRTPTQAQALAQML
                     QEFNAAVSVVPYIAVPEEVLVERLSGRWTCRKNGHVFHEKYNPPTAVGVCDYDGSELY
                     QRDDDLPATVKNRIKVYQEQTAPLIQYYRENGLLVEVDGTKPIDQVTNALLQAIEKAA
                     "
     gene            11546..12349
                     /gene="map"
                     /locus_tag="P5516_04620"
     CDS             11546..12349
                     /gene="map"
                     /locus_tag="P5516_04620"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HRT91755.1"
                     /translation="MSWDRQVTIKTPQEIALMREAGRINAEALAAARALVRPGVTTAD
                     LNAAAEEVLRKYGCYSPFKGYPGPYPYPASTNVSVNHELVHGIPGKRRLKEGDIVSVD
                     CGTVYEGYVGDAAFTEMVGEVSDEARRLVAATEGALFAGIAQMVAGNHLGDVSAAIQA
                     YAEERGFHVTREYTGHGVGRAMHEGPQAPNYGTPGRGMVLRAGMTIALEPMLLVGTPY
                     TKVLPDQWTVSSADGSLTAHFEHTIAVTENGPLILTTQGDGSILDGKWK"
     gene            12399..12512
                     /gene="rpmJ"
                     /locus_tag="P5516_04625"
     CDS             12399..12512
                     /gene="rpmJ"
                     /locus_tag="P5516_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005487032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HRT91756.1"
                     /translation="MKVTTSIRKRCAKCKIVKRSGVLFVICENPKHKQRQG"
     gene            12521..12910
                     /gene="rpsM"
                     /locus_tag="P5516_04630"
     CDS             12521..12910
                     /gene="rpsM"
                     /locus_tag="P5516_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HRT91757.1"
                     /translation="MARIEGIDLPRNKRIEVGLTSIFGIGPTRAREILRSTQVNPDTR
                     VKDLTENEVAALREFISKNYKVEGDLRREVQMNIKRLIEIGCYRGLRHRRNLPAHGQR
                     TRTNARTAKGPKKTVAGRGRRRGAAKK"
     gene            13017..13421
                     /gene="rpsK"
                     /locus_tag="P5516_04635"
     CDS             13017..13421
                     /gene="rpsK"
                     /locus_tag="P5516_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004074888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HRT91758.1"
                     /translation="MAQNVRSTRRTSTTRKVKRTLSSAQVHIFASFNNTIVTVTDQQG
                     NSLCWGSAGSAGFKGSRKSTPFAARLAAEQAIKAAQNMGVQEVDMIIKGPGPGRESAI
                     RAIQSLGLKVRSITDITPVPHNGCRPPKKRRV"
     gene            13443..14081
                     /gene="rpsD"
                     /locus_tag="P5516_04640"
     CDS             13443..14081
                     /gene="rpsD"
                     /locus_tag="P5516_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HRT91759.1"
                     /translation="MAKYTGPACKLCRREGEKLFLKGSRCFSPKCSFEKRGFIPGQHG
                     RTRQGRSERESDYARQLRAKQRARRVYGMMERPFRRYYQVALRTRGLPGLNLLRTLET
                     RLDNVVYRLGYAENRAQARQLVNHGHFEINGRRADIPSMLVKPGDTVTVKETSLKLEH
                     FKALADLAEKRLCPVWLDRDVKKLSGRVLRLPERSEIEGSLNEQLIVEYYSR"
     gene            14133..15098
                     /locus_tag="P5516_04645"
     CDS             14133..15098
                     /locus_tag="P5516_04645"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HRT91760.1"
                     /translation="MCALINMVTPKIEREAEARNYGKFVIAPLERGFGDTLGNSLRRV
                     LLSSLDGAAITSMRISDVTHEFVDIPGVREDVIQVILQIKQIRMILHEGDTSHLHLDV
                     RGEGVVTAADLQVPAEVEIVNPELYLFTVDDAKTKLDFDFTVERGRGYSPATDRSGKL
                     PIGELPVDAIYSPVKRVNFTVGSARVGQSTNYDRLNLEIWTDGTISPEKALASSSKVI
                     IDHLRLISGVSEESILLGAEKEMTGSHLTSEAAETPIENLDLTVRVFNSLKRTGVRSV
                     GDVLELLEKGEEAVMSIRNFGEKSLDELREKMKEKGYLPDEKELE"
     gene            15105..15482
                     /gene="rplQ"
                     /locus_tag="P5516_04650"
     CDS             15105..15482
                     /gene="rplQ"
                     /locus_tag="P5516_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HRT91761.1"
                     /translation="MRHQIAGSKLGRQKDHRAGLRRTMVKQLFEHERIQTTHTKALFI
                     RGEAEQLITLARRSNSGTDAEKVHARRLAAARLGSAAMVRKLFDEIAPRYENRKGGYT
                     RIYKLGPRLGDSAMMVQIELVEE"
     gene            15510..16277
                     /gene="truA"
                     /locus_tag="P5516_04655"
     CDS             15510..16277
                     /gene="truA"
                     /locus_tag="P5516_04655"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HRT91762.1"
                     /translation="MALYKLILAYDGTDFSGFQRQGKTRTVQLVVETVLRELGWQQPA
                     MLFAGRTDAGVHACGQVATISLDWQHSSADLVNALNARLPDDVSVIEANVAQAEFHPR
                     YDASSRTYHYHLYQLPVRQPLLERYAWRIWPELDPERLDRAASRFIGRHDFCAFGRAM
                     KKGGSTTREVYTSQWMKTPEGWRYEVEANAFLYHMVRRMVFIQVQAASGADESEIETA
                     LREGRPLKPGLAPAQGLFLVNVSYAGLTRERCEDEKN"
     gene            16264..16743
                     /gene="rplM"
                     /locus_tag="P5516_04660"
     CDS             16264..16743
                     /gene="rplM"
                     /locus_tag="P5516_04660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HRT91763.1"
                     /translation="MKKINQWRGIYPANGDEKVDKTYVLKGKQEPEWLLIDATGQGVG
                     RLATQIAAYLLGKHKPTFTPGVDMGDAVVVINASKLDITQKRLDEKKYYRHSNYPGGL
                     KTVVMRDQMKNHPDRVITAAVWGMLPHNRMGRALLKRLKVYAGGEHPHAAQNPKPVA"
     gene            16759..17154
                     /gene="rpsI"
                     /locus_tag="P5516_04665"
     CDS             16759..17154
                     /gene="rpsI"
                     /locus_tag="P5516_04665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HRT91764.1"
                     /translation="MSSQYYEGVGRRKESTARVRLMSGNGKFTVNDRPVEEYFPRTGD
                     MEAILGPLSATEQDRATYDITVSVRGGGVSGQTDSVQLGLARALVKLNPENTALLRKG
                     GFLTRDPRVKERKKPGLKRARKAPTYTKR"
     gene            17280..18719
                     /gene="mazG"
                     /locus_tag="P5516_04670"
     CDS             17280..18719
                     /gene="mazG"
                     /locus_tag="P5516_04670"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HRT91765.1"
                     /translation="MEEKRIHNLSVLTTGVEQSAVIQMAGKAHFCLARGESAAAWLAA
                     QGVTTVEQVGEKPSLNFNASQWRERLFARIPSGDTQFSVFYATSIQPALLDRLAGELA
                     GISRTVEWLDGAALPHNLPTQAAGLLGGSLQSIDILALINTDYPVFSPALPVLIHCAG
                     FASQVAHLSSMLAQVYPPEHPLFIRMEKPGSAVPWQETTLAGLQGLPERVDAVFIAPC
                     APDTSLENFMQLIARLRAPDGCPWDRKQTHASLRPYLLEETYEALEQLDKNNMEGLKE
                     ELGDLLLQIALHAQIAAEANTFNITQVLQQINRKIVSRHPHVFGSVSVKNDRDVVQNW
                     EKLKELERSAKGKEQHSSLLDGIPQILPALAQAQSIQERAARVGFDWPDIEPVLAKVL
                     EEMREVSEVQNERERAQELGDLLFAVVNLVRWYGVDAESALRCTNQKFRRRFAYIESA
                     AQMTGRELQNMTLEEMDTYWEESKKLDEP"
     gene            18778..20316
                     /gene="ftcD"
                     /locus_tag="P5516_04675"
     CDS             18778..20316
                     /gene="ftcD"
                     /locus_tag="P5516_04675"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HRT91766.1"
                     /translation="MMTKPLIECIPNFSEARRPEVIGAIIEAIQSVDGVRVLDQHSDL
                     DHNRTVITMVGSPSSIEEAAFQSIKTAAERIDLEQHQGAHPRIGAADVVPFVPISGVS
                     MSDCVELAKRLGKRVGDELSIPVYLYEEAATTPDRQNLENIRKGQYEGLKAEISTLAE
                     RKPDYGPARLGSAGATVIGARAPLIAFNIYLTTSDTEIAQKIAKAVRHSSGGLRYVKG
                     MGVLVEGLAQVSMNLTNFHKTPIFRVVEMVRREAQRYGTQIHHSELVGLIPQSALIDT
                     AVWYTQLDQFDPEQILESRLYSSQEDLTPAPNNPYAFIEDLASGNPTPGGGSAAAFSA
                     AEAAGLVAMVGRVTIGKKSYAAVEAQMREMIEKADDLRANLTSAVEEDAAAFNSLMQA
                     LRLPKANDEEKMRRENAMEEATLQAAMVPHRVAGWALEVLRLSREAVELGNLNAISDG
                     ASASYLAAAAIKSAGMNVKINRSALKNPSAADGLMADLAAYAQEMNEIEQHIARLLQQ
                     RAQF"
     gene            20340..20894
                     /gene="dcd"
                     /locus_tag="P5516_04680"
     CDS             20340..20894
                     /gene="dcd"
                     /locus_tag="P5516_04680"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HRT91767.1"
                     /translation="MGIKADKWIKKMALEKKMIDPFVESQVREGVISYGLSSYGYDIR
                     VADEFKIFTNVNSAIVDPKNFDTRSMVDFQGEVCVIPPNSFALARTIEYFRIPRSVLT
                     ICVGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPAKIYANEGIAQVLFFEGDE
                     VCDISYADRKGKYQKQSSILLPKL"
     gene            20917..22173
                     /locus_tag="P5516_04685"
     CDS             20917..22173
                     /locus_tag="P5516_04685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91768.1"
                     /translation="MKKIPCSVLMKFTGITLWVLLAAQLLSGCSVAQSFMPTETPTST
                     ATPLPTATPLPTSTPTEVTFYLDAAVMPLNQQAPILLYHRFIPDWEAKDAITFTRLSE
                     FKNDLQKLYDSGFSLVSLKSWIDGTYLVPPGKKPLVITIDDFWFADQIYISEDGTPSP
                     YSGIGVLWRFAQEHPDFGFSAAGFSNMGDKYYGDRQIGDKFFYGDNQDAMWLKLGQTI
                     AWAIENGVEPYNHLLQHPMLSKTADKDIQWQIEENDRLTRYYLELAGRGDLIPRLGNM
                     IALPFGEWPATYSGIEIIKKYQNQEGKPVEAIFEAYLYADKELTPSAFSNDFNRYNLA
                     RLTASEIMTQWLLQQAPDLPDAQSCRLGPLAQNQADDQAVIQGLISAAIQSGSCFEGA
                     YHVGEQIFLARDGEVVPYSPPSIIAP"
     gene            22714..23835
                     /locus_tag="P5516_04690"
     CDS             22714..23835
                     /locus_tag="P5516_04690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91769.1"
                     /translation="MRKRLKKLERYNPFKDRTPVQRWNAAQYLMITLLSFAASISGTR
                     LFLEVTGFPRLESGEIHIAHVLWGGLLLFIGALLPLILANEWGLRLSALLTGLGIGLF
                     IDEVGKFITQTNDYFHPVAAPIVYVFFLLTVLLFAFIRNRRKSSSRADMYEILERFNE
                     VLDHDLSTEEYRGLLQRLDNVVAAAEPRSLVELAESMRDYLKANRSRVVPDNPIFIER
                     IRAALQRFEKNWLVPKRFKKVVVLGLLIWGVWALVSPIGYLLITRDAAQLSAFVDQLI
                     SNRLVRNASGLNWFEARVLIEGGVGVLAILSAIMVLFKFEKRGVMLGIVDLLVTLTIV
                     NPLVFYFEQFSTLIVAAFQFILLILLIRYRKRLQRMENE"
     gene            23835..24383
                     /locus_tag="P5516_04695"
     CDS             23835..24383
                     /locus_tag="P5516_04695"
                     /inference="COORDINATES: protein motif:HMM:NF012982.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HRT91770.1"
                     /translation="MEPGNLPYPESYWVVPGKLMAGEYPGKYDSERTRQRVVSLLRCG
                     LNAFIDLTRPADSFFPYLQVLKAEAEDFGLEVTWHNFPIDDFTAPPPKLMRQILDEID
                     AQIASGKTVYVHCFAGIGRTGTVVGCYLVRRGLTGSEALQRINELRKDTPTWWHRSPE
                     SQSQVDLIMDWKDGTNPAADQR"
     gene            24517..25098
                     /gene="rplI"
                     /locus_tag="P5516_04700"
     CDS             24517..25098
                     /gene="rplI"
                     /locus_tag="P5516_04700"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HRT91771.1"
                     /translation="MKVLLLKDVYKLGHAGDVKKVADGYGRNFLLPQGLAVLATAGAL
                     KTVEKIRERAAVERAKLNQEMSGVAELLQDLELQFFSKAGETGKLYGSITSQMIVDEI
                     HNKLAISLDRHQIEVAPIRTLGEHLATVRLTVDLNPKIKVLVNREGEAAKVSARKQKV
                     EEPIIEAAAVVEETITSAAEETMATEETEEKAE"
     gene            25185..26579
                     /locus_tag="P5516_04705"
     CDS             25185..26579
                     /locus_tag="P5516_04705"
                     /inference="COORDINATES: protein motif:HMM:NF024855.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4115 domain-containing protein"
                     /protein_id="HRT91772.1"
                     /translation="MTVGEYLRRIREEKQISLEQVSSGTNIRVYYLEAIEADNWEQLP
                     SQAQGRGFARLYAAYLGLSSRDVFDEVEQMARHELGGAPETEPALKQPAAILVERPQP
                     FGQKPGKKRSSLSATSRERNEEALSPVTAPPVSQRIFNEIGVELRRQREALGLSLDDV
                     ERLTKIREFYVFALENGRVDDLPSSVQGRGMLNNYASFLSLNADALQMRYAEALQQRR
                     LEKLEQERVEKKEGVAVMGKEPLTGWRRFLTPDLLVGGMVFFVLFSLVVWGAVKMINT
                     SIPRTEPTISSISEILVGETEDITALTDEPTTQLTEDTPEATQWINVPQTDMQATIAA
                     VEPGPIQVVVVAYQRAYMKITVDGNEKFSGRVIPGNVYAYTGTYKISLITGNAAALQV
                     YYNQQDLGVLGPLGQVAEIEFTRQEMMTPTPRFSPTPSLTPLPTLTALPTNTPMPTAT
                     VPTPTITPALTIKP"
     gene            26590..27924
                     /gene="rimO"
                     /locus_tag="P5516_04710"
     CDS             26590..27924
                     /gene="rimO"
                     /locus_tag="P5516_04710"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HRT91773.1"
                     /translation="MKRNSYYLVSLGCAKNSVDSQSMAVLLDRDGYKAVEKPAQAEVL
                     IVNTCGFIEQARQESMEVLADLARSKKTGQLLIAAGCFSQLYRERLVEMVPGIDGLIG
                     TRRWMDIATYLHELRAHPGEPRYHLPENPTIGTDEQGVPRVSLQGASAYLKIADGCRR
                     PCAFCSIPLIKGLQVSRPLETILHEAELLQSHGIKELILIAQDTTDYGSDLGMKDGLV
                     RLLEQLLPRIPAIPWVRILYAYPGYVTDGLIDLMSSAHQILPYLDMPLQHASVPVLRA
                     MKRPSNMDWVRRTIEKMRARIPDLALRTTFISGYPGETDAHHQELLEFIREMTFDHLG
                     VFPFSFEPGTVSARLGDPVPAEVKLQRVEEIMRLQEQLSLRKNQRFIGSTLDILVEGH
                     GDGISVGRSYRDAPEIDGLVLIDEEVPVGEMTPVTITGALTHDLSGHLYRPR"
     gene            complement(27937..29295)
                     /locus_tag="P5516_04715"
     CDS             complement(27937..29295)
                     /locus_tag="P5516_04715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91774.1"
                     /translation="MKPVIDEGATIPVKTAVKRKPRPAASSLVLDADATIPVVPSPAR
                     KPRSVTSQAPTAEGATAPIKKRNQPTPKPATAQTAPALRNPKKSAVLSQPVFKDSAQT
                     QPFAGSGEEPPVETEPPAGKMKHGKWIWLGLLGMLILILFGAGVGYASAIRARIIEQT
                     NQRLIAATTQFELGLVDQREGRLETAKKRYEYVLGIYPEFPGITDKLVEVGLALAESQ
                     GSAEAVPTQDPGQPEIVITPVATKDTTSVSILFNQAQNQLKAKDWPGLYTTLEKMRYI
                     DPEYEAVKVDGMLYLALRNHGIAQIQAGHLEPGLYSFALASKIAPIDTDAAGYFDWAS
                     RYLKAASYGDVDEDNPVLHTYAVSQFSELHSLVPNLRDASGITVSQRYAQALVYLGDF
                     YQRKGDYCSAVPNYQQAATIFSLPALAEKIPQAEEFCQNPPETPTPTPDPSAPTATPE
                     QG"
     gene            complement(29431..30969)
                     /gene="murJ"
                     /locus_tag="P5516_04720"
     CDS             complement(29431..30969)
                     /gene="murJ"
                     /locus_tag="P5516_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HRT91775.1"
                     /translation="MKFSRLTKISLLLAAFFFLDKVLAFARQVLIARQFGLSAELDAF
                     NVANNIPDLLFALISGGALAIAFIPVLSEVLTRSGQKPAWLLFSRIANLAFLVTVALA
                     TLVAVFAAPMVQAELGIAPGFTVAQQHLVIHLMRLNLVATIIFSISGLVMAGLQANQH
                     FLLPAMAPLFYNIGQILGVGILSPEQGYRLGPLTLPAYGLGVQGLVYGVIIGAVLHLA
                     IQVPGLAKYHFKWSISLNLRDEAVRKVLRLMGPRLLTMFFIQLIFLIRDNLASRLAEG
                     AVTSLTYGWMIQQVPETLIGTAIGTALLPTLSEHFSRDEKQAFSERIDKAFKALLALT
                     LPVTAVLAAGLRPLLGLAFDFGAAGTRMLAWVTAAFMLGLFGHSLKEVSARAFYASQN
                     AWVPLATAGGNVLVYTLCGALFYRPLGAPGIALTDSLIFSLEALVLLALLIRSGRVSI
                     VPSSTACRALLGCVSAGAVTWLGVTLLEAHAHPLISGSVPMFAGILVSLPWIWREIKL
                     LTQL"
     gene            31119..31796
                     /locus_tag="P5516_04725"
     CDS             31119..31796
                     /locus_tag="P5516_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012799.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HRT91776.1"
                     /translation="MTKKKGTLYIVATPIGNLGDITLRAIETLKQVNTVICEEHKPGS
                     TLIKKLQLTGKELVLLNEHNEAEVAAQLLTRLLNGESLALISDCGTPVFSDPGAYLIQ
                     LASSAGVVVSPLPGASSLMAALSLLDTRIERFVFAGFLSRDPHKRKQELTRYRGFGMP
                     VVIMDTPYRLTALLEDVIKVFGKGQWVMVAFDLTLPGEMVFRGEAESVAKQVGGKKGE
                     FVLVVGR"
     gene            32469..32618
                     /locus_tag="P5516_04730"
     CDS             32469..32618
                     /locus_tag="P5516_04730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91777.1"
                     /translation="MKHNLLWLWIVLGILAVIAIVILCLPKTKRRFLSYFLRQVPSLI
                     ARYFV"
     gene            32628..34133
                     /locus_tag="P5516_04735"
     CDS             32628..34133
                     /locus_tag="P5516_04735"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRT91778.1"
                     /translation="MTQYDAVIIGAGLGGLTTGAVLAKRGKKVLVLEQSGQIGGCCTT
                     YEVGKYKYDIGASVVEMLPIIDGVFAELGTTFEKEVDLIQCDPIYSVIFPDNSRLTIP
                     MSVEGAADEIGKISPEDRKGFLEYAKTFEAFIEGNGLDFFTMPMNTLGDFMGAFKKIP
                     VLAKFNKYFFMTYGDVLNKFFKSPKIKQSMAYQSFYSGHSPEQAVGIFAIIPYMEHKG
                     IYYPRGGMIKIPQAIQRLGEKSGLEVRVNAEVVKVIVDDSRRAKGVVLKNGEKIEAKV
                     VVSNINARKLYIDLVGEENLPSIVKTGIKSYNLSKSTPYICLGVDYDPPLDAHHTLVT
                     RDVEEINAFWWEKYRKGYLPTPQEQFGLICCPTKADPSLAPKGHHIVALDFQGPFKLA
                     GQTWDDVKEEFSESLVSYLSERIIPGLKDHVQDMTVSTPLDFERRLLNPGGAFYCFQQ
                     DLSAQAVFRPASKSKAIKGLYLAGASTHPGGGVPTVMASGVIAANQIVQYE"
     gene            complement(34141..>34531)
                     /locus_tag="P5516_04740"
     CDS             complement(34141..>34531)
                     /locus_tag="P5516_04740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91779.1"
                     /translation="LLFPIFWLKPFTALSPLIYLSFEYRTGKTLTTDAIYRETPGRRK
                     HHLTDGCDVVEVEATAFFTMVQFREVVLGQVVYGGDLVMDGRGWHKRKDDQNIMFGMA
                     VKAALLLKKKEAASIRIETASFSPTTL"
BASE COUNT         8685 a         8528 c         9687 g         7631 t
ORIGIN      
        1 tcgcgattcg tgaaggtgga ctgacggttg gcgccggcag gatcacaaaa atcattgcat
       61 aggttaataa catggcaaaa caaaaaattc ggatacgctt gaaggcctac gatcatcgtg
      121 tgctcgatca atctgccaaa aggattgtcg agaccgcgga gcggagtggc gcgcaggttg
      181 taggacctgt tccactgccg acccgcattg aacggttcac tgttcgccgt tcatcattca
      241 tcgataaaga ttctcacgag cattttgaga tcagaacgca caaccgcctg atcgatgtgc
      301 tcgatcctga cgccaagacg attgacatgt tgatgcgttt gaacatgccg gctggtgtgg
      361 atattgagat caagatctaa gaccttactg cgcatctgaa ctttgcattg tgcggtagat
      421 caccttaaaa attaaaaaaa cccttacaaa taccggaact tgcggtataa tgtatcggtt
      481 ttgggcgtaa gcccaagcag gcaattcaag agcggttctt gtgggcgcac cagacaaaaa
      541 ccgatagtgt cgcagcaaac tgctaactga attgttcagg gatgatgcag ccaagcccag
      601 gctgacagtc tctactaaag tactgaagga gaacaactga aatgtttaaa gggctgatcg
      661 gaaagaaaat cggcatgacc cagattttcg atgaaaatgg cgcggcgcac cctgtcacgc
      721 taatcgaagc tgggccatgc ttcgttaccc aggtgagaac cgtgcagaag gaaggttact
      781 ccgcggtgca gctgggttac gaagaagtaa aacccaggcg tctggccagc ggcgagttag
      841 gtcacttgcg acgcaatagc cttcccccat taaaatacct gcgcgaattt cgggccaaag
      901 aggttcaggt tgctgaaggc gacaaagtgg atgtgaacct gttcgcggtt ggtgaaaagg
      961 tggacgtctc gggaatcagc aaggggaagg gatttgcagg cggtgtaaaa cgctatcatt
     1021 tccgcggtgg tccaaaatcg catggtcagt cagaccgtat gcgggcgccg ggttcgcgtt
     1081 cttcaaccac cacacctggc agagtattca aaggcgctcg cggtgccggt catatgggca
     1141 atgacgctgt aaccgcgcag aatttgaagg tcgtcctggt ggacgcggaa cgcaatgtca
     1201 tcggtgtcaa tggctccgtg cctggggcac gcggtggcct cgttctcatt aaagaatcgc
     1261 ggaaaggtta ggtccgaggt tttttatccg tttacctgga ctaagggagc tcatgacaat
     1321 ggaagttgaa gtcttaaaca tggaagggaa aaaggtcagt aaggtggaac tgccaccggc
     1381 gatctttgaa gccccaatca atattgatct gatgcatcag gcttacgtga tgcaaatggc
     1441 gaacgctcgc cttggaacgc acgagaccaa ggtcaagagc gaggtttcag gcggtggcag
     1501 aaaaccatgg cggcaaaagg gaaccgggcg tgccagacag ggttctacta gatcggctca
     1561 gtggaaaggc ggcggtaaga ttcatacccc gcatatgcgc tcttacgttc aaaacatgcc
     1621 gaagaaaatg cgccaggccg cgctgcgttc agctctctct gtgaaagcgg ctgaatcctc
     1681 aattgtggtg gtggacgccc tcaaacttaa ggaacccaag acgcgtttaa tggccagcgc
     1741 gttgaatacg ctggttggcg atgccaaagc ccttgtgttg attccttcag cagaatccta
     1801 cgaggatgtg ctgcgctcca ccaataacat ccctgacgcc aaggtattga atgccaatta
     1861 catcaatgtg cgcgacatta tggtctttga taagctgatc atgcctgtcg ccgcccttga
     1921 gttaattacg gctaacctgg gaggggtagg agaataagat gagcactatt tacgatgtcc
     1981 tccgccgccc gattgtgacc gaaaaaacca attacatggt cacgaaactt catcagtacg
     2041 tcttcgaggt ggcagatgat gccaaccgga caatggtgaa agacgcggtt gaaaaattgt
     2101 tcaacgtgaa tgttttgcgg gtgaatatca tcaatgcgcc ggcgaaaagg tcccgccgcg
     2161 cgcgcagccg aaggctattg atccgcaacg ctgagtacaa aaaagcggtc gtcacgctca
     2221 gccctgaaga ccgtattccc atctttgaag gggtggagta atggctatta aggtatacaa
     2281 accaaccaca cccagccggc gcgcaatgac cggttacagc tttgaagaaa tcaccaaaga
     2341 cacgcctgag cgcgctttga tcgtgatccg caaagcccgg gctgggcgca acaatcatgg
     2401 aaaagtgact gtgcgccacc agggcggcgg cagcaagcag tatatccgca tcatcgattt
     2461 taaacgtgat aagcgggaaa ttcccgcacg cgtggccgcg attgaatatg atcccaaccg
     2521 tactgccaga ctggctctgc tgacttatgt tgatggtgag aaacgttata tcatcgcgcc
     2581 ggttggtctg aaagtaaacg atacagtggt agccggtcca atggctgagg taaggcctgg
     2641 caacagcctg cccatttcca atattccggt cggcaccctt gttcataata tcgaactgaa
     2701 agaagggaaa ggcgggcagt tggtgcgctc agcgggcgcg tctgcgcagt tattggcaaa
     2761 agagggcgat ttcgctcaaa tccgtatgcc gtcaggcgaa gtccgcctgg ttcccgaaaa
     2821 atgttacgcc actatcgggc aggttggaaa cacagaccat agcaacatca agctcggtaa
     2881 ggcgggtagg aaacgccata tgggcatcaa gcccaccgtg cgcggcacgg ctatgagccc
     2941 ccgcgaccat cctcatggcg gtggtgaagg acgccagccg gtcggtatgt ctgggccgaa
     3001 aagcccgtgg ggtaagccca cgcgcgggta tcgcacccgg cgtaacaaaa agaccgacca
     3061 gtatattgtg cgccgccgca acgccggcaa tcggtaaaac aagccgcagc gcttaaccga
     3121 gaagaaggga cgagaatatg tctagatcgc taaaaaaagg gccgttcgtt gacccgaaac
     3181 tattgaaaaa aattgaagcc atgaacaccc gcagcgagaa gaaagtggtt cgcacctgga
     3241 gcagggcgag cacgatcttc ccgcaaatgg tgggccacac catcgctgtg catgatgggc
     3301 gccggcatgt tcccatttac atcacagaga atatggtcgg tcaccgtttg ggtgagtttg
     3361 cccccacgcg tcatttccgg ggacacatgt ctgataaatc taccggagcg agataattat
     3421 ggcaacagat attcgcgcag aattgaagaa ttgtggtgtt tctgctcaga aaacgcgcct
     3481 ggttatcgat ctgatccgcg gcaaaccggc agttgaagcg ctgaccactc tcaaatttac
     3541 caataaggca gctgcaaggc atgtgggtag attgctggct tccgccgtgg cgaacgctga
     3601 agagaacttc ggcgtcaacc gtgatgatct ggtggtttac aagatcagcg ctgatgatgc
     3661 ccctcttcgc aaatggcgcc gatttggcgc ccgcggacgc ttcaagccgt tgttgagacg
     3721 ctcctctcat atcactgttg tgctgagaga gaaggaatag ataccaggag ggcggcaggg
     3781 aaaacctccg tctgagtgaa aaaggacacc aggagaaatt atgggtcgaa aagtacatcc
     3841 aattggtttt cgtcttggaa tcaaccagcc ctgggaaggg cgttggtacg ccgagggttc
     3901 tgattaccgc acccagcttc accaggatct ggtcatccgc aggatggttc agagccaggg
     3961 agaaaaggcg ggaatctcgt tggttgagat cgagcggttc ccgggcaagg taaaggtagt
     4021 ggtgcacacc gcgaaacccg gcatccttat tgggcgcaag ggagattctg taaaaaagat
     4081 ccgcactgat cttgaagcgc ttactggcaa ggcgattgac ctcgagatca aggagatcaa
     4141 agcgccggat tgtgacgcgt ttctcgttgc taagaacatc gccggtcaga tcgaacgcag
     4201 ggtaagctat cgtcgcgcga tgaagcgcgc gctgcagcaa gccatgcggc agggcgccga
     4261 gggaattaag gtctcggtct ctggtcgcct gagcggcgct gaaatggcac gtatcgtctg
     4321 gctgcgtgag gggcgcgtac cacttcagac gctgcgtgcg gatatcgact ttgcccgcgc
     4381 ggaagcgtta accacgtacg gaagaatcgg tgtgaaggtt tgggtttata aaggtgaagt
     4441 gatccccggt gttgaagaga aggttgaacc caccgagggt gtgtacgtga gtgaatagat
     4501 tctaaccccg gaaaactccc gggtattgtg aaagaggtag cagctatgtt gatgccaaaa
     4561 cgtgttaaat ggcgcaaagt gatgcgcggt cgcatgaccg gcaaggcgca gcgcggcgca
     4621 gacatgatcg tgggtgagta cgcactccag gcggtggaac ctggttgggt gaccgcccgg
     4681 cagatcgaag ccgcccgccg caccatcgtg cattcaatga aacgccatgg taaggtgtgg
     4741 atccggatct tccctgataa accctatacc caccgcccac ccgaaacccg tatgggcaaa
     4801 ggcaaaggca acgtggaata ctatgtcgcg gtggtgaagc ctggcaggat catgttcgaa
     4861 atcagcggcc ttgagcgtga agaagccatt cacgctttga accaggccgg ttataaattt
     4921 tcaatcaaga ccaaagtgct ggcccgtggg caggaagccg gggagggtgt atgaaaacct
     4981 ctgagattcg cttgttatcc acagaagaga tcaagaccaa actcgctgat acgcgcaacg
     5041 agttaatgaa cttccgcttt cagctggtca ccggtcaatt gaccgatacc agtcgattga
     5101 aaaccacccg gcggcaaatt gcgttgtacg aaacgatttt acgcgaacgc gagcaaggca
     5161 agaaactgga aggtgcaaaa tgaaccaaag acgtagattg acagggatcg tcacaagcaa
     5221 ccggatgatg aagacagtgg ttgtcgaaat tgctcgcacg tacaaacacc cgctatatca
     5281 caaagtggtg cacgcccggc aaacaatcaa agcgcatgat gaactgggtt gcgcgattgg
     5341 cgataaagtg atggtggtgg aatgtgcccc gatgtcgaaa gaaaagacct ggatggtgca
     5401 gtccatcctc aagcgcgaag ggaaaacgct tgatgtggct gaggaggcgg ttgaatgatt
     5461 caacaggaga ctcgtgtaaa ggtcgccgac aacaccggcg cgcgggaact tcttgtcatc
     5521 caggtggtgg gcggttccag gcggaaatat ggtgcggttg gagatgtggt tattgccact
     5581 gtcaagtcag cagcccctca ggggtcggtg aaaaagagcg aaatcgtcaa agcggttatc
     5641 gttcgcgcct ccaaagagtg gcgccgcgaa gatggctcat tcatccgctt tgatgacaac
     5701 gccgcggtga tccttgatgt ggatggtcag aacccgaagg gtacacgcat cttcggtcct
     5761 gtcgcgcgcg aactgcgtga acggggctat atgaagatca tctctctggc gccagaagta
     5821 ctctaacaga gggataggag caattattat gaaagtaaaa attcgtaagg gtgatcaggt
     5881 ggaagtaatc agcggcacca tcaatgataa gggcaagcgc ggcgaagtga tcaaagtgct
     5941 gccggatgag aaccgcgtgg ttattcaggg tgtgaacaca cgcaccaagc atcagcgcca
     6001 ggtccaatca cagggtcgca cagtcaaccc gggcctcgtc cgttttgaag cccctatcag
     6061 catttccaac gtggcaatcg tgtgcaaaaa atgtaataaa tctacgcgcg ttggcctggt
     6121 acgcgaaggc gataaaacaa agcgtcaatg caaggaatgc ggcgcactga ttgatgaata
     6181 agccgattga gagagggttc taatgaattt aatgaaagaa cgttatatta aagaagtctc
     6241 accggcgttg atgaaatcac tcggcttatc caatgtgatg cagataccga ggattaagaa
     6301 agtggtcatc aatatcggca tgggcgaggc gatggaaaat cccaaagcgt tggatgcggc
     6361 agtgcaggac ctgagcgcca tcaccggtca aaaaccagtg atcaccaagg cgcgcaagag
     6421 catcgcgaac ttcaaactgc gcgaagggcg cgcaattggt actgcggtca cgctgagggg
     6481 agaaaagatg tgggcttttc tggatcgatt gatgaacatt gtcttgcccc gtgtgcgtga
     6541 tttccgcggc gtgtcggcgg agtcttttga cggcagaggt aactacacac tgggtttaag
     6601 ggagcaaatc atattccctg agatcgagta tgacaaagtc gataaagtac gtggtatgga
     6661 aatcaccatc gtaactaccg caccatctga tgatcaagcc gcagccctgc tgcaactgct
     6721 tggcatgcct ttcaggaagg aatagtatgg caaagaaatg catggaatat cgtgaattaa
     6781 gacgggaata tcccaatcag gtgcgcaacc gctgtaaacg ctgcggtaga ccgcgcggtt
     6841 acctgcggcg ctttggttta tgccgcattt gcttcagaga gctggctctc gaaggcgaaa
     6901 ttcccggcgt gaccaagtca tcctggtaat gccaattcgg aggaagaaac atgagtgtaa
     6961 atgatccaat tgctgatatg ttgacccgta tccggaatgg cgtcattggc agccaatcag
     7021 tggttgccat gcctcactcc aaggtcaaag cggaaatcgc ccgcatcttg aaggaggaag
     7081 gtttcattga aggatatgaa gtggtggaag gcgacaaacc ggggatgatg actttgcgca
     7141 tcaagatcaa gtatgtgggt gaacggcgtg aaaaaacacc cgttatcacc ggtcttgagc
     7201 gcatcagccg ccctggccgc cgtgtctaca gtaaaaaaca agacatcccc tgggttttgg
     7261 cagggatggg tatcgcaatc ctatccaccc caaagggtgt aatgaccgga caaagagctc
     7321 gccagctcgg aattggtggc gaagttcttt gcaaggtttg gtagcccctg ggatgaagga
     7381 ggatagatat cgtgtctcgt ataggtaaat tacccatcag aatacctgat tcagtccagg
     7441 ttgaaattaa cggttctgag gtaacagtaa aaggaccgaa gggtgaaatg aagcgccagt
     7501 tttctcctga tatcatcatc acccaggagg atgggatgct gttggtcaaa cgcaattctg
     7561 acctcgcgcc agtgagggca ttgcacggta ccacgcgcgc ccttctcttt aacatggtca
     7621 ccggtgtgtc taccggtttc accactgaat tggaagtgga tggagtggga tatcgcgtgg
     7681 aaatggaagg cgaaaaactg gtattgtttg ttggttattc gcatcccttc aaaattgacc
     7741 cgccaaaagg cgtgcacttt gagatcgaca gcaagaccca cctggtgaag atcaatgggt
     7801 cagataaaga ggtcatcggc caactggcga atgacattat caagatcaga ccacccgaac
     7861 cctatctggg caagggtata cgttacaaag gtcaaaagat acgccgcaaa gccggtaaag
     7921 ccggcaaggc gaaggcataa agcaggtgag ggaatatggc aaaaaataat cgtacagaag
     7981 caagaattca cagacaccgc cgcgtgcgca agtacatcag cggtactgaa tcccgacctc
     8041 gcctcagcgt atttcgcagc ctggctgaaa tttacgccca ggtaatcgat gatcaggccg
     8101 gcgtcacact tgccgccgcc tcaagcatcg accatgaatt gcgcggcaaa acggacggaa
     8161 agaaaaagct ggaccaggcg aaactggtag gcgagctgct cgcccggcgc gcaaaagaca
     8221 aaggcattac ccgggttgtg tttgaccgcg gcggttttcg attcagcggc cgtgtgaaag
     8281 cgctggcaga cgcggctcgt gaagccggtc ttgagtttta gggtagaagg aagagataga
     8341 gggctcattt tatggatatg atggattatc aaccccagga aacgcagttt gacgaacggg
     8401 taatcgagat tgccagagtc gcaaaagtgg ttaaaggcgg ccggcgcttc cagttcaggg
     8461 tcactgttgt tgtcggagat aataacggca aaattggtat cggtattggc aaggcgaacg
     8521 ccgtgccaga cgcgatgcgc aaagccaccg aacgcgccca caagaacatc cgcaaggtca
     8581 cgctcaccgg cacgaccatc ccgcatgaag ttacgggccg cacagccggg gcggttgtac
     8641 tgctcaaacc tgcctcagct ggtactggtg tgatcgctgc tggcggcgtg cgcgcggtac
     8701 ttgaagcagt tggcgtgcac gatattctga ccaagtcatt aggcagcgca aacgtattga
     8761 acgtggtgaa agccactttc gacgcgctcg cgcaattgaa atcccccgaa accgaggcag
     8821 cccgccgcgg aaaacccgtg aaagaactga tgccttactg ggaacggagg aagaatgcct
     8881 aagaaaaaag aagcgttgaa aaaggtgcgc atcacgttgg tgaaaagcgc catcggttat
     8941 tccgaaacgc ataaagccac catccgcgcg ctgggtctgc gccgcatgaa tcaaactgtc
     9001 gaacaggttg attcaccggt gattcgcggc atgctgatga aagtcaatca tcttgtaaaa
     9061 atcgaagagc aggaagtcaa atgaaattac acgatctaat gccaaacgaa ggggcgaaaa
     9121 aagccccgaa acgaaaaggt cacggtatct ctgcggggca gggaaagacc gccggacgcg
     9181 gcaccaaggg tcttggcgct cgtgcaggat ctggggggca cctttatcgt cagggtggta
     9241 acctgccttt ttatcgccgc ctccctttca tccgtggtga gggctttacc cctcccaacc
     9301 gggttgaatt caacgaggtc aatcttgatc aattagagcg cttccaggcg ggcagcatcg
     9361 tctctcgcga aaccctgcag gaaaacgggt tgctccacaa agccagcaac cctgtggccc
     9421 tgcttggccg cggcgaggtt aaggttgcgc taaccgtgca ggtgcaacga gtttcaaaat
     9481 cagctcgtga gaagatcgaa gccgctggcg gcaaagtaga gattgtcgcc tgaacgccat
     9541 cctaaggagt gttgaatgaa gcgatctgcc tggcgttacc tttggacatc tgaagacatc
     9601 cgcaagaagc tgttgattac tgtgctcttg ctggtgatct accggctggc agcgaatatc
     9661 cctgttcccg gtatcaaccg cgaggtcatc cgctcaattt cagagatggg tggaccggga
     9721 gcatcgcttt ttaacctgct cgacctgctg tcaggcggaa ctatttcgaa cttttccatc
     9781 cttgcgatgg gtgtataccc gtacatcacc tcgcaaatta ttatccagtt gctggtgccc
     9841 atcatccctg cccttgaccg caggctgaaa gacaacccgc gcgaaggccg gtctttgatg
     9901 gaaaaatgga cgaccatgct gaccattccc atggcgtttc tttcagccat tgggcaggtc
     9961 aacatcttca attcgatggc aaaaaccggc agcgtgctga ccacgccttt tgggtttgtc
    10021 ggcgcttcat tgttaccgac gattacgctg ctattgagca tggtggcggg cacgatgttt
    10081 ggtatctggc tgggtcaact catttctgaa tttggtattc ccaaccaggg tttatcgttg
    10141 atcatcttcg ccggtatcat ttcaaaaatt ccttccaacc tgttgggtat cctggcagat
    10201 aaagaaaatg gctggtgggt gctgatcatc atcctggtcc tgctggcgct cacaattttc
    10261 gccatcgttt acgtgcagca gggcagacgc aatgtacccg tgctgttccc tggaaggcgc
    10321 attggcaatc gcatgtcgat gcccgtgaga agcaatttac cgctcatggt caacatggca
    10381 ggtatgatcc cgcttatctt cgcgcaaacc ttcctgtcgt tcccggcgct gatcgccagt
    10441 tacttcatcg ccggtaaaac ttccggtttt tggtacggcg tttactccct gttcagcggt
    10501 agtggctggt tctacccgat cattttcttt gtcctggtcg tggcgttcac cttcttctac
    10561 acagatgtgt tgttcaccca acagaattac ggtgatacgc tcaaaaagca gggtgcgcaa
    10621 attccggggg tggttcgtgg tggaccaacg caaaaatacc tcaccagggt gcagcgccgc
    10681 ataactttcc cgggcgcatt cttcctcggg tttgtggctg ttttacccta tatcatcggc
    10741 acgatcctgc caattaccag ggcgagcgcc tttgtaattt cagccgcagg cctgttgatt
    10801 gtagtcggcg ttgtgcgtga taccttcacc atcatcgaaa ccgaactcaa agttcatggt
    10861 tacgacgaaa gtattatcaa aggttaatat ggctgtgtac attgttcttc ttggaccacc
    10921 aggcgctgga aaaggaacgc aagcccaact catcgcgcaa aaatatgggt tgatgcatgt
    10981 ttcttctggc gatctcttca gggagaattt gaagaacgaa acagagctgg gaaaactcgc
    11041 ccagacgttc atgcagcgtg gtgaattggt tcctgatgac gtcacgattg ccatggtacg
    11101 cgaacggctc ggccgcccag attgtcaaaa aggcgcgctc ttagacggtt ttccacgcac
    11161 tccgacacag gctcaggcgc tggcgcaaat gctgcaggag ttcaacgcag ccgtcagtgt
    11221 agttccgtat atcgctgttc ccgaagaggt actggtggaa cgattaagcg gtcggtggac
    11281 ctgccgtaag aacgggcatg tattccatga aaaatataat ccaccaacag cggtcggcgt
    11341 gtgcgactat gatggttctg agctttacca gcgggatgac gacttgccag ccactgtcaa
    11401 aaaccgcatc aaggtttacc aggaacagac cgccccgctg attcaatatt accgtgaaaa
    11461 tggactgctg gtcgaggtgg atggcaccaa gcccatcgac caggtaacga atgctctttt
    11521 acaagcgatc gagaaagccg cgtaaatgtc ttgggaccga caggtaacca ttaaaacgcc
    11581 gcaggaaatc gccctgatgc gggaagcagg cagaatcaat gctgaagcgc tggctgctgc
    11641 gcgcgcgttg gtgcgtcctg gggtgaccac cgctgacctc aacgctgcag ctgaagaagt
    11701 gctgcgtaaa tacggctgtt acagcccttt taaaggttac cctggtcctt atccttaccc
    11761 cgcgagcacc aatgtgagcg tcaaccatga attggtgcac ggtattccag gcaaacgcag
    11821 gttgaaagaa ggggatatcg tttcggtgga ttgcggcact gtctatgaag ggtatgttgg
    11881 agacgcggcg ttcactgaga tggttggcga ggtgagcgat gaagcacgcc gcctggtggc
    11941 tgccaccgaa ggagcgctgt tcgccggcat tgcccaaatg gtggcaggaa atcacctggg
    12001 ggatgtctcg gcagccatac aggcatacgc cgaagagcgc ggattccacg ttacccgtga
    12061 atataccggg cacggcgtgg gaagagcgat gcacgaaggc cctcaagcgc ccaattatgg
    12121 caccccgggc agagggatgg tactgcgcgc cggcatgacc atcgcgctgg aacccatgtt
    12181 gctggttggc acaccttaca ccaaggtact gcctgaccag tggacggtat cttcagcaga
    12241 tggttcgctg accgcgcact tcgaacatac aattgcggta acggagaatg gacctttgat
    12301 cctcactacg cagggagacg ggtcgattct ggacggaaaa tggaagtagt agtaaaattg
    12361 aaaattcgga ttaagaacga gagcgaggaa cagatattat gaaggtaaca acatccattc
    12421 gcaaacgttg tgcgaaatgt aaaatcgtga aacgcagcgg cgttctgttt gttatttgcg
    12481 aaaatcctaa gcacaagcag cgacaagggt agaggaactt atggcgagaa ttgaaggcat
    12541 tgatctaccg cgaaacaagc ggatcgaagt tggtctgacc agcatttttg gcattggtcc
    12601 taccagagca cgtgaaatat tgcgttctac tcaggttaac cctgataccc gggttaaaga
    12661 cctgactgaa aatgaagttg cggctcttcg agagtttatt tcgaagaact ataaagtcga
    12721 aggtgacctg cggcgcgaag tgcagatgaa catcaagcgg cttattgaaa ttggttgtta
    12781 ccgcggtttg cggcaccgcc gtaacctgcc ggctcatggg caacgcacac gcaccaacgc
    12841 ccgtaccgct aagggtccta agaagactgt tgccggtcgc ggacgccgcc gtggagcagc
    12901 gaagaagtaa tctgctttag aaccagtccg ggtggggtgc cttctccccc cgcggcagcc
    12961 cggtcaggct ctttagtaga aactaacgtg atttagaaaa aggtaaacga ggtaatatgg
    13021 ctcaaaatgt tcgttcaacg cgtcgcacaa gcacgacccg caaagtgaaa cgaaccctgt
    13081 catcagccca ggtgcacatt ttcgcgtctt tcaacaacac gattgtgacg gtgactgatc
    13141 aacagggcaa ctcgctctgc tggggaagcg caggttcagc cggattcaaa ggctcacgca
    13201 agagcacacc cttcgccgcc cgtcttgcgg ctgaacaggc gattaaggct gcgcaaaata
    13261 tgggtgtcca ggaagtggac atgatcatca aagggccggg acccggcagg gaatccgcca
    13321 ttcgcgctat tcaatccctg ggtttaaaag tgcgctcgat tacggatatc acaccggtac
    13381 cgcataatgg ctgccggcct ccgaaaaaac gccgcgtgta attaaaagga gaaggttaag
    13441 atatggccaa atatactggt ccggcttgta aactttgccg gcgtgaaggt gaaaaattat
    13501 tcttgaaggg cagccgttgt ttctcgccca agtgttcttt cgaaaaacgg ggttttattc
    13561 ccggccagca cggacgcacc cgacagggtc gctctgagcg tgagtcggat tacgcccgcc
    13621 aattgcgcgc caaacaacgc gcccgccgtg tatacggtat gatggaacgc cctttcaggc
    13681 gttactacca ggtcgcactg cgcacgcgcg gtctcccggg tttaaacctg ctgcgcacgc
    13741 tcgaaacacg cctggataac gtggtatacc gactggggta cgcggagaat cgcgctcagg
    13801 cccggcagct cgtaaaccac ggtcattttg agatcaatgg gcggcgcgca gatatcccct
    13861 cgatgctggt aaaaccaggt gataccgtta ccgtaaaaga gacctcatta aagctcgaac
    13921 acttcaaagc acttgctgat ttggctgaaa aacgattatg ccccgtctgg ttggacaggg
    13981 atgtgaaaaa actttccggt cgagtgctca ggctaccaga acgctctgag atcgagggca
    14041 gcctgaacga acagttgatt gttgagtact actcacgtta aagcgggcta aaaggatgaa
    14101 aggatacaag tgagtatcgg aaagggtgta ttatgtgcgc tttaatcaat atggtcacac
    14161 ccaagattga acgcgaggca gaagcccgca attacgggaa attcgtcatc gctcctttgg
    14221 agaggggttt tggcgatacg ctgggtaact cattacggcg tgtgctgctt tcatcactgg
    14281 atggcgcggc gattacctct atgcgtattt ctgatgtgac gcacgaattt gttgatatcc
    14341 ccggtgttcg ggaggatgtt atccaggtga tcttgcagat caaacagatt cgtatgatcc
    14401 tccacgaggg cgatacctcc catttgcacc tggatgtgcg gggcgaggga gtggtaacgg
    14461 ctgctgattt acaggtgccg gctgaagttg aaatcgttaa ccccgagttg tatctcttta
    14521 cggtggacga tgccaagacg aagctggatt ttgacttcac tgtggagcgg ggacgcggtt
    14581 attctcccgc gaccgatcgc agtggaaaac tgcccattgg cgaattgccg gtagacgcca
    14641 tctacagccc ggttaaacgc gtcaacttca cggtgggctc cgctcgcgtt ggtcagagca
    14701 cgaattatga ccgtcttaat cttgaaattt ggacggacgg cacaatttca cctgaaaaag
    14761 cgctggcttc ttcgtcaaag gtcatcattg atcatttaag gttgatttcc ggcgtgagcg
    14821 aagagagcat tctgctcgga gctgaaaaag aaatgacagg cagccatctc acaagcgagg
    14881 ctgctgagac tccgattgaa aacctggatc ttaccgtgcg agtgttcaat tcgctcaaaa
    14941 gaactggcgt gagatcggtg ggcgatgtgc tggaattatt ggaaaagggc gaagaggctg
    15001 ttatgtcaat ccggaacttt ggcgagaaaa gcctggatga gctccgtgaa aagatgaaag
    15061 aaaaaggcta tttgcctgat gaaaaagagt tggagtaggt aaagatgcgc catcagattg
    15121 caggttcaaa attaggacgt caaaaagacc atcgcgctgg tttgcgccgc acgatggtga
    15181 aacaactgtt cgaacacgaa cggattcaaa ccacccacac caaggcttta tttatacgtg
    15241 gagaagcaga acaattaatc acgctcgcgc gccgcagcaa cagcggtacg gatgctgaaa
    15301 aagttcacgc gcgcaggctg gccgccgccc gattgggttc cgcagccatg gtcagaaagt
    15361 tgtttgatga aatcgccccg cggtacgaaa accgtaaagg cggttatacc cgaatctaca
    15421 aacttggacc ccgccttggc gattccgcca tgatggtcca gatcgagctg gtagaagaat
    15481 aatccttccg ggtttccgga tggattggca tggcacttta caaacttatc ctcgcctacg
    15541 acggtactga tttctcaggt tttcaacgcc agggaaaaac acgaactgtc cagttggtgg
    15601 ttgaaactgt tcttagggag ctgggctggc agcaacccgc catgcttttc gcaggccgca
    15661 ccgatgcagg tgtgcacgcc tgtggacagg tggcgacaat ctcactggat tggcaacact
    15721 catctgctga tctggtaaat gcgttgaacg cccgcttacc cgatgatgtt tcggtgatcg
    15781 aagccaatgt ggctcaagcg gaattccatc cccgttatga cgcttcaagc cgcacgtacc
    15841 attatcacct ttaccagtta cccgtgaggc agcccctttt ggagcgttac gcctggcgca
    15901 tctggcctga attggacccg gaaaggctcg accgggcagc aagcaggttc attggcaggc
    15961 atgatttttg cgcttttggc agagccatga agaaaggggg cagcacgaca cgcgaagtgt
    16021 ataccagcca atggatgaaa accccagaag gttggcggta cgaagtcgaa gcgaacgcgt
    16081 ttctttatca catggtccgc cggatggtct ttatacaggt gcaagccgcc agcggggctg
    16141 atgaaagtga gattgaaaca gcattacgag aaggccgccc gttaaaaccg ggcctggcac
    16201 cagcccaggg gctctttctg gtaaatgtga gttatgcagg tcttacgcgt gagcgttgcg
    16261 aggatgaaaa aaattaatca atggcgaggg atataccccg ccaatggaga tgagaaagtg
    16321 gacaaaactt acgtcctcaa aggaaaacag gaacctgaat ggttgctgat cgatgcgacc
    16381 ggtcagggtg tggggcgcct ggcgacccag attgcagctt acctgctcgg taaacacaaa
    16441 ccgactttca cccccggggt ggatatgggt gacgcagtgg tggtgatcaa cgcgtcaaaa
    16501 ttagacatca cccagaagag gcttgacgaa aagaaatatt acaggcactc aaattacccg
    16561 ggtgggttaa aaacagtggt aatgagagac cagatgaaga atcacccgga tcgtgtgatt
    16621 acggcggccg tatggggcat gctgccacat aaccgaatgg ggcgcgcttt attgaagcgt
    16681 ctcaaagttt acgctggcgg agagcatccg cacgcggctc aaaacccgaa accggttgct
    16741 taaaggagct ggaaagatat gtcgagtcag tattatgaag gtgttggacg ccgcaaagaa
    16801 agtaccgccc gggttcgcct gatgagtggc aacggaaaat ttaccgtaaa tgaccgtcca
    16861 gtagaagaat attttccgcg cacgggcgat atggaagcca tcttgggccc gttgtcagcc
    16921 actgaacagg accgagcgac ctacgatatc acagtgagtg ttcgcggcgg tggtgttagc
    16981 ggccagaccg attcagttca attggggttg gcacgcgcgc tagtaaaact taaccccgaa
    17041 aacaccgctc tactgcgtaa aggagggttc cttaccaggg acccgcgtgt aaaagaacgc
    17101 aagaaaccag gtctcaagcg ggcgcgtaag gctcctacgt acaccaaacg atagtccctg
    17161 tctggatttc atcctgatat ggatgagatg cggtcatcag gtgcgcctgg acaccagcga
    17221 tcatcattga tccggtcaaa ggcccgcagg atgaccgcgt cttgcttttt cgagagctta
    17281 tggaagaaaa acgaatacac aatttgtcag tgctgactac aggggtggag caaagcgccg
    17341 tcattcaaat ggccggcaaa gcgcattttt gcctggcgcg cggcgaaagc gctgctgcat
    17401 ggctggcagc acagggcgtt acgacggtag agcaggttgg agaaaagccc tcattgaatt
    17461 tcaatgccag ccaatggcgc gagcggcttt tcgccaggat tccctccgga gatacgcagt
    17521 tctccgtttt ttatgcaaca tccatccagc ccgctttgct tgaccgcctg gccggagaac
    17581 tggcaggcat ctccaggaca gtggaatggc tggacggcgc tgcgttgcca cacaacttgc
    17641 caacgcaggc agctggactg ctgggcggtt ctttacaatc catcgatatt ttggccctta
    17701 taaatacaga ttacccggtt ttttcacccg ctttacctgt gctgattcat tgcgcggggt
    17761 tcgcctctca agtggcccat ctcagcagca tgctggcaca ggtttacccg cccgaacacc
    17821 cgctttttat tcgtatggag aaacccggga gcgctgttcc ctggcaagaa acaacgctcg
    17881 caggactgca gggtttaccc gagcgggttg acgctgtttt catcgcgccc tgcgcgccgg
    17941 atacctcgct ggaaaacttt atgcaattga tcgcccggct gcgcgcaccg gatggctgcc
    18001 cttgggacag aaaacagacc catgcgagtt tgcgcccata cctgttggaa gaaacttacg
    18061 aagcgttgga acagcttgat aaaaacaaca tggaggggtt gaaggaagaa ctgggcgacc
    18121 tgctgctgca aattgcgcta catgcccaaa tcgcagcaga agcaaatact ttcaatatca
    18181 cgcaagtatt gcaacaaatc aaccgaaaaa tcgtttcaag gcacccgcat gtatttggtt
    18241 cagtgagcgt aaaaaatgac cgggatgtgg tacagaactg ggaaaagctc aaggaacttg
    18301 agcgttctgc taaaggcaaa gagcagcatt ccagccttct ggatggcatc ccgcagatcc
    18361 tgcccgcgct ggcgcaggcg caatccattc aggaacgcgc tgcccgggtg ggctttgact
    18421 ggccggacat tgagccggtg cttgccaagg tgctcgaaga aatgcgcgag gtcagcgaag
    18481 tgcagaatga gcgggaacgc gcgcaggaac tgggggattt gctcttcgct gtggtgaatc
    18541 tggtgcgctg gtatggtgtg gacgcggaat ctgccttgcg ttgcaccaac cagaaattcc
    18601 gccggcgctt tgcatacatc gaatcagcgg cacagatgac tggtcgcgag ttgcaaaaca
    18661 tgaccctgga agagatggat acgtattggg aagagtccaa gaagctggat gagccctgag
    18721 caggaaatat ggcagaaaat gcctgggtta taatttgaca aacataaaac gagggcaatg
    18781 atgacaaaac ctttgatcga atgcatccct aacttttcag aagcccgccg gcctgaagtg
    18841 attggcgcca tcatcgaggc catccagtct gttgacggtg tgcgggtttt agaccagcac
    18901 tctgacctgg accataaccg cacggtcatc accatggtcg gcagtccatc ttcgattgaa
    18961 gaagcagcct ttcagtcgat aaaaacagcc gcggaacgca tcgaccttga gcagcatcaa
    19021 ggagcacacc cgcgcattgg ggcggcagat gtcgtgccat ttgtccccat ctctggggta
    19081 agcatgagcg attgtgtaga actggcaaaa cgcctgggta aacgggtggg agatgagcta
    19141 tcaatacctg tttacttata tgaagaagcc gcaaccacac ctgaccggca aaaccttgaa
    19201 aacatccgta aggggcaata tgaaggattg aaagccgaaa ttagcacact ggcggagcgg
    19261 aaaccggatt atggtcctgc caggctgggc agcgcaggcg caacggtgat tggcgccagg
    19321 gcgcctttga tcgcttttaa tatttacctc actacctctg atactgagat cgcgcaaaag
    19381 attgcgaaag cggtgcggca ctcatcaggg gggctgcgtt acgtgaaagg tatgggcgtg
    19441 ctggtggaag ggctggcgca ggtttcgatg aaccttacga attttcataa aacgcccatc
    19501 ttcagggtgg ttgaaatggt gcgacgtgaa gcacagcgtt acggcacgca aatccatcac
    19561 agcgaactgg ttggtctcat tccacagtcc gcgctcatcg atacagcggt gtggtatacc
    19621 cagttagatc agttcgaccc agagcagatt cttgaaagcc gcctgtattc ctcgcaagag
    19681 gacctcacgc ccgcgccgaa taacccgtat gcttttattg aagacctggc gagtggcaac
    19741 cctaccccgg gtggcggttc agccgccgcg ttcagcgctg ctgaagcggc cgggttggtg
    19801 gcgatggttg ggcgagtgac catcggcaaa aaatcttacg ccgcggtgga agcacaaatg
    19861 cgcgagatga tcgaaaaagc ggatgatttg cgggcaaacc tgaccagcgc ggttgaagag
    19921 gacgccgccg cttttaacag cttgatgcag gcgctgcgtt tgccgaaggc gaacgatgaa
    19981 gaaaaaatgc ggcgtgaaaa cgcgatggag gaagccacac tgcaggctgc aatggtgcct
    20041 caccgtgtgg ctggttgggc gctggaagtg ctgcgccttt ctcgcgaagc ggtggagttg
    20101 ggcaacctca acgctatttc ggatggggct tctgcctcct acctggctgc cgcggcgatt
    20161 aaaagcgccg gcatgaacgt gaagatcaac cgcagcgccc tgaaaaaccc gtccgcagcc
    20221 gatggattaa tggctgatct tgccgcgtat gcacaggaga tgaatgagat cgagcagcac
    20281 atcgctcggc ttttacagca acgggcgcag ttttaggtta gaaacacagg agttttcata
    20341 tgggcatcaa ggctgacaaa tggattaaaa agatggcgtt ggaaaagaag atgattgacc
    20401 ctttcgtgga aagccaggtg cgcgaaggag tcatttcgta cgggttatct tcatacgggt
    20461 atgatatccg cgttgccgat gaatttaaaa tatttaccaa cgtgaactct gccattgtag
    20521 atcccaagaa cttcgatacg cgctctatgg tggattttca gggcgaggta tgcgtgatcc
    20581 cgcccaattc tttcgcgctg gcacgtacga ttgaatattt ccgcatcccg cgctcggttc
    20641 tcaccatctg cgtgggaaaa agcacttacg cgcgctgcgg aatcatcgtc aatgtgacgc
    20701 cctttgaacc cgagtgggag ggctatgtga cgctggagat ctccaatacc acgccgctgc
    20761 ctgccaagat ctacgccaac gaggggatcg ctcaggtgtt gttctttgag ggagacgagg
    20821 tttgcgatat ttcttacgca gaccgcaagg gaaagtacca aaaacaatcc agcattctct
    20881 tgccaaagct ctgaaaccgg atatacttgg tcggatatga aaaagatacc ttgtagtgtt
    20941 ttgatgaagt tcactgggat aaccttatgg gtgttattgg ctgcccagct gctttcaggc
    21001 tgctcagtag cgcaatcctt tatgcccact gaaacaccca ccagtacagc cacgccgttg
    21061 cccactgcca cgccgctgcc cacttccacg ccgaccgaag tgactttcta ccttgatgcc
    21121 gcggtgatgc cgttgaacca acaggcgccg atattgctct atcatcgctt tatcccggat
    21181 tgggaggcca aggatgctat tacttttacg cggctttccg agtttaagaa tgacctgcaa
    21241 aagctttatg acagcggatt ttcgctggtt tcccttaaga gctggataga cggcacttat
    21301 cttgtgccgc ccggtaaaaa acccctggtc atcacaatag atgacttctg gtttgcggac
    21361 caaatctaca tcagcgagga tggcacgcct tccccatact cgggaatagg cgtactatgg
    21421 cggttcgcgc aagaacatcc tgattttggg tttagcgcag ccggtttttc aaatatgggg
    21481 gataagtatt atggtgaccg gcaaattgga gacaagttct tttatggaga taatcaagac
    21541 gccatgtggt taaaacttgg gcaaaccatc gcctgggcga tcgagaatgg cgttgaacca
    21601 tataaccacc tgctccaaca cccaatgctg agcaagacag ccgataaaga tattcaatgg
    21661 caaattgagg agaacgacag gcttacccgt tactacctgg aactcgcggg cagaggcgat
    21721 ctgatcccac gattgggaaa tatgattgcc cttccctttg gggagtggcc agccacctac
    21781 agcggcattg agatcatcaa aaagtatcaa aaccaggaag gcaagcctgt tgaagcgata
    21841 tttgaagcct atctgtatgc cgataaagaa ctcacaccat cagccttttc aaatgatttt
    21901 aatcgttata acctggcgcg gttaaccgca tctgaaatta tgacgcaatg gttattgcaa
    21961 caggcgcctg atttgcccga tgcgcagtct tgccggcttg gcccgctggc gcagaaccag
    22021 gcggacgacc aggcggtgat ccagggttta atttccgcag cgatccagag cggcagttgt
    22081 tttgaaggag cgtaccatgt aggggagcag atctttctcg cccgtgatgg cgaagttgtt
    22141 ccttactcgc ccccttcaat tattgctccg taagaaataa ggcttgccaa atgaacatga
    22201 aagccgctga attttcagcg gcgtaatttt gtataattac gatgccgctg ttttctggag
    22261 ggaggatgtt tcgaattcaa tacgctatcg agtatacatg cgaggaggcc cccttcaccc
    22321 ctgagcgtaa tgtcattacc tgggtgtggt gcacgcttcg cggcatggcg tatgaggcgt
    22381 tcgatttgtg ccaggcactc gggcgtcgac aggatacgca taagagcgta gtacgtccac
    22441 tcggttcgct tttcaatgat gggggtttgg tcgtgaagcc tcctaagcct tgtgagttgg
    22501 ttgccaaccc tgcgagccgc aaacaggatt tcaacacaat agactgggag tatcgagaga
    22561 cttcacgaag agcaaacgcc tatatacggt cggggggcat tgtcagcctg ctgttgacaa
    22621 tcttctccag tatggagaac gctcagttcc gctcgattcg gcattcttcg tgttcatcgg
    22681 cggtaacaga aacaggcatt gacaggttcc atcatgcgta aaagactgaa aaaactggag
    22741 cggtataacc cgttcaaaga ccggacgccc gttcagcgct ggaacgccgc ccagtacctg
    22801 atgatcaccc tgttgagttt tgctgcttca atcagcggca ccaggctctt tctggaggtg
    22861 acgggatttc caaggcttga aagcggtgag attcacattg ctcatgtatt atggggaggg
    22921 ttgctgctat tcattggcgc gctgctgccg ttgatattgg ccaacgagtg ggggctacgc
    22981 ctttcagcgt tgttgaccgg gttgggaatc gggctcttta ttgatgaagt gggaaaattc
    23041 atcacccaga ccaatgatta ttttcaccct gtggccgccc ccatcgtgta tgtctttttt
    23101 ttgctcaccg ttttgttgtt cgcattcatt cgcaaccggc gcaaatcttc cagcagggca
    23161 gatatgtacg aaattctgga gcggttcaat gaagtgctgg accatgatct ttctacagaa
    23221 gaatatcgcg gcttgctgca acgtttagat aatgtggttg cagccgctga gcccaggtca
    23281 ttggtggagc tggcagagag tatgagagat tacctcaagg caaaccgctc cagggtagtg
    23341 cctgataacc cgatatttat cgagcgcata cgcgctgcgc tgcagcgatt cgaaaagaac
    23401 tggcttgtgc ctaaacgttt taaaaaagtg gtggtgcttg gccttttaat ttggggagtt
    23461 tgggcattag tgtcgcctat cggctatctg ctgatcacca gagacgccgc gcaattatca
    23521 gcgtttgtag accagcttat ttctaaccgg ctggtgcgca acgccagtgg attaaactgg
    23581 tttgaagcca gggtgctcat cgaaggaggg gtgggtgtac tggccatact gagcgccatt
    23641 atggtcttgt ttaaatttga gaaaaggggc gtaatgctgg gaattgttga tctcttggtg
    23701 acgctcacta ttgtcaaccc gttggtgttc tactttgaac agttttccac cttgattgtg
    23761 gcagcatttc aatttatcct cttaatattg ctcattcgat accgcaagcg ccttcaaaga
    23821 atggagaatg aataatggag cccggtaacc ttccctatcc tgaatcctac tgggttgtac
    23881 ccggtaagct gatggctggt gagtacccag gtaaatatga cagcgaacgc accaggcagc
    23941 gggttgtttc cctgctgcgc tgcgggctga acgcgtttat tgacctgacc agacccgcag
    24001 attctttctt tccttacctt caagtattga aggcagaagc agaggatttt ggattggagg
    24061 tcacctggca taattttccc atcgacgatt ttaccgctcc gccgccaaag ttaatgcggc
    24121 agattctgga tgagatagat gcgcaaatcg cttcaggaaa gacggtgtac gtgcattgct
    24181 tcgctggaat cggcaggaca ggaacggtgg tgggctgtta tttggttcgc aggggtttaa
    24241 ccgggtctga ggcgctgcag cgaattaatg agctgcgcaa ggatacacca acctggtggc
    24301 accgcagccc cgaaagccag tcgcaggtag atttgatcat ggattggaag gatgggacga
    24361 atccggctgc cgaccaacga tgatcgatga atcagcagcc aacatggcgt ctatcttggt
    24421 gtctatcttg gttgaccaac ctggcgcgtt gcggtaaaat ccctgttcac atgcgttgta
    24481 cccttcgcat gaattctgct ttgaaagggc ttgaacatga aagtactgct tttaaaagat
    24541 gtatacaaat tgggacatgc tggggatgtc aagaaggtag cggatggata cggccgcaac
    24601 ttccttctcc cacagggtct cgcggtgctt gccaccgccg gagcgctcaa gaccgttgag
    24661 aaaatccgtg aaagggcagc tgttgagcgc gccaaactga accaggagat gagcggagtg
    24721 gctgagctcc ttcaggacct ggaattgcaa ttcttcagca aagcgggtga gactggcaag
    24781 ctttacggtt ccattaccag ccagatgatc gtggatgaga tccacaataa acttgccatc
    24841 tcattggaca gacaccaaat tgaagttgcc cccatccgca cattgggtga acacctggcg
    24901 acagtacgtt tgaccgttga cctgaacccg aagatcaaag tgctggtcaa ccgcgaggga
    24961 gaagctgcca aggtttctgc caggaaacag aaggttgagg aacccatcat tgaagccgcc
    25021 gcggttgtgg aagaaacaat cacttccgca gccgaagaaa caatggcaac cgaagaaaca
    25081 gaagaaaaag ctgaataagg cgttttacct ggattgccca aatgaccggc caaatcagcc
    25141 aggggctgag gccggtcttt cttaaaatcg gttaagatta agacatgaca gttggtgaat
    25201 acctgcgccg tataagagaa gaaaagcaaa tctcgctgga gcaagtttcc agtgggacaa
    25261 atatccgcgt ctactatctc gaagccatag aagctgataa ctgggaacaa ttaccatctc
    25321 aagcccaggg aaggggattt gcccgtttgt acgcggctta cctgggattg agttctcggg
    25381 atgtgtttga cgaggttgaa cagatggccc ggcatgaact cgggggggcg cctgaaaccg
    25441 aacccgcgct taagcagccc gctgccattt tggtcgaaag accacaacct tttggccaga
    25501 aaccaggcaa aaagcggtca tcgttaagcg ccacctcccg ggaaagaaat gaagaagcct
    25561 tgtcacctgt gacggctccc cctgtttctc agcgcatttt caatgaaatt ggtgtggaac
    25621 ttcggcggca acgcgaagca ctgggcttat ctctggacga tgtagaacgg ttgacaaaaa
    25681 ttcgcgaatt ctatgtattc gcgttggaga acgggcgggt ggatgacctg ccttccagcg
    25741 tgcaaggcag gggaatgctg aacaattacg cgtcttttct tagcctgaac gctgatgcat
    25801 tgcaaatgcg ttacgccgaa gctttgcagc aacgccgcct tgaaaagctg gagcaagaaa
    25861 gagtagaaaa aaaagaaggg gtggcggtga tgggcaagga accgcttacc ggctggcgca
    25921 gatttttaac acctgacctg ctggttggcg gtatggtctt cttcgttctg ttttcgctgg
    25981 tcgtttgggg tgcggtcaag atgatcaaca ccagcatccc gcgtacagag ccaaccatca
    26041 gttcaatttc ggagattcta gtgggcgaga cggaagacat caccgcgctg actgatgagc
    26101 ccaccaccca gttgactgaa gacacacctg aagcaaccca gtggatcaat gttccgcaga
    26161 cagatatgca ggcgaccatc gccgcggtcg aaccagggcc aatccaggtg gtcgtggtgg
    26221 cgtatcagcg ggcttatatg aaaatcaccg tggatggcaa tgaaaagttc agcggtcgtg
    26281 tcattccggg gaacgtttac gcttacacgg gtacctacaa gatttcgctt atcaccggga
    26341 acgcagctgc cttgcaggtg tattacaacc aacaggacct cggcgtgctg ggcccgttgg
    26401 gacaggtggc cgaaattgaa tttacccgcc aggaaatgat gacgcccacg ccgcgctttt
    26461 caccaacgcc ctctttaacg ccgcttccca ccctgaccgc cttaccgacc aataccccca
    26521 tgcctaccgc taccgtaccc acgcctacca tcaccccggc gctcaccata aaaccctgag
    26581 gaaaactgga tgaagagaaa ttcttattac ctggtttcac tgggctgtgc caagaactcg
    26641 gtggattcgc aatccatggc cgtcttgctt gaccgggatg gatataaagc ggttgaaaaa
    26701 cctgcccagg cggaggtgtt gattgtgaac acctgcggat ttattgaaca ggcgcgccag
    26761 gaatcgatgg aagtgctggc tgacctggcg cggagcaaaa agactggcca gttgctcatc
    26821 gctgccggct gtttcagcca gttataccgc gagagactgg tggagatggt gcctggcatc
    26881 gatggattga ttggcacgcg ccgttggatg gatatcgcta catacctgca tgagctgcgc
    26941 gcccatcctg gagaaccgcg ctaccatctt cccgaaaatc ccacgattgg aacagatgaa
    27001 cagggagtac ccagggtttc actgcaaggc gccagcgcct accttaaaat tgcggatggc
    27061 tgccgccgac cctgcgcttt ttgctccatc cctctgatca aaggactaca ggtgagccga
    27121 ccgctggaaa ccatcctgca tgaagcggaa cttcttcaat ctcatggcat caaagagctg
    27181 atcctcatcg cgcaggatac cactgattat ggctctgacc tggggatgaa agatggattg
    27241 gttcggctgc tcgaacaact tctgccgcgt atccccgcaa tcccctgggt gcgcattctt
    27301 tacgcctatc ccgggtatgt gacggatggg ctgattgacc tgatgagcag cgcgcaccag
    27361 atactgcctt acctggatat gccgctgcag cacgcttcag tgccagtatt gagggcgatg
    27421 aaacgcccca gcaatatgga ctgggtgcgc cggaccatcg aaaaaatgcg cgcgcgcatc
    27481 cctgacctgg cgctgcgcac tactttcatc tcagggtacc ctggtgaaac tgacgcacat
    27541 caccaagagc tgttggaatt tatccgcgaa atgacttttg accacctggg tgttttcccc
    27601 ttctcctttg agccgggcac cgtttcggcg cggctgggcg accctgtacc cgcggaggtc
    27661 aaactgcaac gggtggaaga gatcatgcgc ctgcaagaac agctctccct gcggaaaaac
    27721 cagcgtttta tcggttcgac gctggatatt ctggtggagg ggcatggcga cggaatttca
    27781 gtagggcgca gttaccgtga cgcgcctgaa attgatgggc tggtgctcat tgatgaagag
    27841 gtgccggtag gggagatgac cccggtgacg atcaccggcg cgttaacgca tgaccttagc
    27901 ggtcatctct accgtcccag gtaaaaaaca acaggatcag ccctgttcag gcgtggctgt
    27961 gggcgctgat ggatccggtg tgggggttgg cgtttcaggc ggattttgac agaattcttc
    28021 ggcctggggg atcttctccg cgagcgctgg cagggagaaa atggttgcag cctgctggta
    28081 atttggtacg gcgctgcagt aatctccctt gcgctgataa aaatcaccca ggtagaccag
    28141 ggcttgcgcg tagcgttgag agacggtgat acccgaggcg tcgcgcagat tgggcaccag
    28201 gctgtgcagt tctgaaaatt gagaaaccgc gtaagtgtgc agcacggggt tatcctcgtc
    28261 tacgtctcca taagaagccg ctttgagata ccggctggcc caatcaaaat atcccgcggc
    28321 atccgtatct ataggcgcga tctttgacgc cagcgcgaaa ctgtaaagcc cgggttcgag
    28381 atgcccggcc tggatttgag cgatgccgtg gttacgcagc gccaggtaga gcatgccgtc
    28441 tactttcacc gcttcatatt cagggtcaat atagcgcatc ttctccaatg tggtgtaaag
    28501 ccctggccaa tctttagcct tcaactggtt ttgcgcctgg ttaaagagga tgctgaccga
    28561 ggtggtatct tttgtggcta caggggtgat gacaatttcg ggctgaccgg ggtcctgtgt
    28621 gggaaccgct tccgcacttc cctggctttc ggcgagcgcc aggccaactt ccaccagttt
    28681 atcggtgatg cctgggaatt cgggatagat gcccaacacg tattcataac gctttttggc
    28741 ggtctcgagc cggccttcac gttgatcgac caatccgagc tcaaattgcg tggtggctgc
    28801 gataagacgc tggttggtct gctcgatgat cctggcgcga atggcagatg cgtagcccac
    28861 gcctgccccg aacaatatca ggatgagcat gcccaacaat cccaaccata tccattttcc
    28921 gtgtttcatc ttgcccgcgg gaggttcggt ttcaacaggg ggttcttcgc cgctgcctgc
    28981 gaaaggttgt gtttgcgcag agtctttgaa aacgggctgg gataaaacag ccgatttttt
    29041 tgggtttcgt agagcaggtg cagtttgcgc agttgctggt ttgggcgtgg gctgattgcg
    29101 ttttttgatg ggagcggtcg ccccttctgc ggtgggcgct tgagaggtca cgctacgagg
    29161 ttttcgcgct ggagagggga ctaccgggat ggtcgcgtca gcatctagta cgagtgagga
    29221 tgcggctggg cggggttttc gttttaccgc agtctttaca gggatggtag cgccttcgtc
    29281 tatgactggt ttcaaccacg ctccatcagc atggtttggt tgcgtatcct ctgtggagga
    29341 gggttgaatt ggttgggtgt tatcctggtt tgttttcgac atgagcgtaa ttataccgta
    29401 agccagtgga gggtcaaatt gctgcgtttt tcataattgg gtgagcagtt ttatttcgcg
    29461 ccagatccag gggaggctga ccaggatgcc ggcaaacatg ggaactgaac cagatatcaa
    29521 cgggtgcgca tgcgcttcga gcagggtcac ccccaaccac gttacagcgc cggcgcttac
    29581 gcatcccagc agcgcgcgac aggcagtgga gctgggaaca atcgacaccc tgccactgcg
    29641 tatgagcagc gccaataaca ccagcgcttc cagtgaaaaa atcaacgaat cggtgagggc
    29701 gatgcctggc gcgcccagag gacggtaaaa cagcgctccg caaagcgtgt aaacgagcac
    29761 attaccccct gcggttgcca gcggcaccca ggcgttttga ctggcataga aggcgcgcgc
    29821 gctgacctcc ttaagggaat gaccgaacaa gcccagcata aacgcggcgg tcacccaggc
    29881 gagcatacgg gtacccgcag cgccaaaatc gaaagccaag ccgagcaaag gcctgagccc
    29941 tgctgccaga acagctgtga cgggcagcgt gagcgccagc agcgccttaa aggctttgtc
    30001 tattctttcg ctgaacgcct gcttttcatc gcggctgaag tgttctgaaa gcgtgggtaa
    30061 aagagccgtg ccgatggcgg tgccaatgag cgtttcaggc acctgctgga tcatccagcc
    30121 ataagtcaac gaggtaaccg ccccctctgc caggcgtgat gccaggttat cgcggatgag
    30181 gaagatgagc tggataaaga acatcgtcag caggcgaggg cccatcagac gcagcacctt
    30241 gcgaaccgct tcgtcacgca ggttcaggct gatgctccac ttgaaatggt attttgccag
    30301 cccgggaacc tggatggcca ggtgcaatac cgcgccgatg atcaccccat aaaccagccc
    30361 ctgtacgccc aacccgtagg caggcagggt gagcgggcca agcctgtaac cctgctcagg
    30421 cgaaaggatg ccgaccccca agatttgacc gatgttgtaa aaaagcggcg ccattgctgg
    30481 cagcagaaaa tgctggttcg cctgcagacc ggccatcacc aagccgctga tggaaaagat
    30541 gatggtcgcg acgaggttca agcgcatcag gtggatcacc aggtgctgtt gcgcaacggt
    30601 gaaaccggga gcgataccca gttcagcctg gaccattggc gcggcgaaga ccgccaccag
    30661 ggtggccaat gcgacggtga caaggaaagc caggttggcg atgcggctaa agagcagcca
    30721 ggcgggtttt tgccctgacc gggtcagcac ttcagaaagc acagggataa aggcaatggc
    30781 cagcgcaccg cctgagatga gcgcgaaaag caggtcaggg atgttattag ccacattgaa
    30841 ggcatccagc tcggcagaaa gaccgaactg tcgagcgata aggacctgac gcgcaaacgc
    30901 tagcaccttg tcaaggaaga aaaaagcagc cagaagcaac gaaattttgg tcaggcggga
    30961 gaatttcata tctgcaccag agatgaaatg gtaacataaa aaatgaaatc agagacgatt
    31021 ttgccaggag agagtaagcg gcatgggagg aaaaaccggc ggtgaagaat cggttacaat
    31081 ggttttggct gccataaatc cgaaagggag aggatgattt gaccaaaaag aagggcacct
    31141 tgtacattgt cgccacacct attgggaacc tgggcgacat cactctacgc gcgatcgaaa
    31201 ccttgaaaca ggtgaacacg gtgatatgcg aagagcataa accgggcagc acgctgataa
    31261 aaaaactgca actgaccggc aaagaactgg ttctcttgaa tgagcacaat gaagccgagg
    31321 tcgccgcgca actgctgacg cggctgttga acggtgaatc gctggctttg atttctgatt
    31381 gcggcacgcc ggtattttct gacccggggg cataccttat ccagctggct tcaagcgcgg
    31441 gagtggttgt ttcgccgctg ccgggggcgt catccttgat ggcagcgctt tcgttgctgg
    31501 ataccaggat tgagcgcttc gttttcgctg ggttcctctc acgcgacccg cataaaagga
    31561 aacaggaact gacacgatac cgtgggtttg gtatgcctgt ggtcattatg gatactcctt
    31621 accggctgac cgcattgctg gaagatgtga taaaggtgtt tggcaaaggt caatgggtga
    31681 tggtggcatt cgacctcacc ctgcctgggg agatggtctt ccgcggcgaa gcagaaagcg
    31741 tcgccaaaca ggtgggcgga aagaagggcg aatttgtact ggtggtggga aggtaaaagt
    31801 tccgggcaga tcgagcctaa aaacacccga taaacggtta tacatggaag tacgctgcac
    31861 gaaaaaaggg ctgaaggtaa ctgctggcag caaagtctgt gtgtagaaaa agaaggctta
    31921 ccgggattcc acgtagtcaa atcagtcaaa tgacagaatt tggatagaat cccaactatt
    31981 ggtaaaaaga caagcttttt gaatcaaaat tacttgaatt tatagccaaa ataaccactt
    32041 attacctact ttcgatgctt atttggtttt tcggcattcg ccggatttca ctaaatagtg
    32101 aatcggatat ttttggtaga gatacaatgt caaaattttt cgtgtaagaa aagatgtgtc
    32161 ttgttgtaac tctcaacaaa atatccttaa ctagaaatcc atgcaaaaac ttttcctgaa
    32221 aaaaaacggt attatgtcct aatctgttgt ttacttttaa gcaagaccga gaaaaatcat
    32281 ttcctgcatc tgattgtatg aattggatcg caaaaatcgc tttacaatgg aggatacaat
    32341 gctatactca aatgtataat gtctcaacca ccacccttct ggggtgaatt tgttggcaaa
    32401 aatcaacaaa cgtggacttt cttcaatatt cggcatttta gtattcaaat ctatctagcg
    32461 gagggatcat gaagcacaat ttgctctggt tgtggatcgt actgggcatt cttgccgtca
    32521 tcgcaattgt cattctctgt ttgccaaaaa ccaagaggcg ctttttaagt tattttttgc
    32581 gccaggttcc gtcactcatc gcacgttact ttgtatagga gacaaccatg acccaatatg
    32641 atgcagtaat catcggcgcc gggttaggtg gattgaccac tggagccgtc cttgccaaac
    32701 gaggtaaaaa ggtgctggtt ctcgagcaga gcgggcagat tggcggatgc tgtaccactt
    32761 atgaagtggg aaaatataaa tatgatatcg gagcctccgt ggtggaaatg ttgcccatca
    32821 ttgatggcgt tttcgctgag ttgggcacca cctttgaaaa agaagtggac ctgatccagt
    32881 gcgacccgat ctactcggtg atcttccccg ataattcgcg tctgaccatt cctatgtccg
    32941 tggaaggcgc cgccgatgag attggaaaaa tctcacccga agaccgcaag ggatttttgg
    33001 aatacgcgaa gactttcgaa gcctttattg agggaaatgg tctcgatttc ttcaccatgc
    33061 cgatgaacac cttgggtgat ttcatgggcg ctttcaaaaa gatcccggtc ctcgcgaaat
    33121 tcaacaagta tttcttcatg acctacgggg atgtgttgaa caagttcttc aaaagcccca
    33181 agatcaaaca gagcatggcc taccaatcct tctacagcgg gcattcaccc gagcaggccg
    33241 tgggcatctt tgccatcatt ccctacatgg aacataaagg catctactac ccacgcggcg
    33301 gcatgatcaa gatcccgcag gccatccaac gcctgggtga gaaatccggt ttggaagtgc
    33361 gcgtgaatgc ggaagtcgtg aaagtgatcg tggatgacag ccgccgcgcc aaaggtgtgg
    33421 tcttgaaaaa tggcgagaag atcgaagcca aagtggtcgt ctcgaatatc aatgcccgca
    33481 aactctacat cgacctggtg ggagaagaga acctgccttc gatcgtcaag accggcatca
    33541 aaagctacaa tctatcaaaa tccacaccct acatctgcct cggcgttgat tacgacccgc
    33601 cgctggacgc gcaccacacc ctggtgacgc gcgacgtgga ggagatcaat gccttctggt
    33661 gggaaaaata ccgcaaaggc tacctgccta cgccgcagga acagttcggt ctgatctgct
    33721 gccccaccaa agccgatcca tcgctggcac ccaagggaca ccacattgtc gcgctggatt
    33781 tccagggtcc atttaaactg gcggggcaaa cctgggacga cgtcaaggag gagttcagcg
    33841 aaagcctggt ctcctacctg tcggagcgca tcattccggg tttgaaggac cacgtgcagg
    33901 atatgaccgt ttcgacgccg cttgatttcg aacgcaggct actaaacccg ggcggcgctt
    33961 tctactgctt ccaacaggac ttgagcgccc aggccgtttt ccggccagct tccaagtcaa
    34021 aggccatcaa gggtttgtac ctggccggtg cttcaacgca tcccggcggc ggagtgccga
    34081 ccgttatggc ttcaggtgtc attgccgcca accagatcgt ccaatacgaa tgaagattca
    34141 ttaaagggtg gtaggggaaa aagaggctgt ctcaatcctt atcgaggcag cctctttctt
    34201 tttcagcaac agggcggctt ttaccgccat cccaaacata atattttggt catccttgcg
    34261 cttgtgccat ccacggccat ccatgaccag atcaccgccg tagaccacct gcccgagcac
    34321 aacctcgcga aactgcacca tcgtaaagaa ggcggtggct tccacctcaa ccacgtcgca
    34381 gccatccgtt aggtggtgtt ttcgcctgcc gggagtctcg cgataaatgg cgtcggtggt
    34441 caaggtttta ccggtgcggt attcaaagct tagataaatt aaaggcgaca gagcggtaaa
    34501 cggcttaagc cagaagattg gaaagagaag c
//
LOCUS       DAOTLW010000020        33484 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1570_length_33484_cov_11.049478, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000020 DAOTLW010000000
VERSION     DAOTLW010000020.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 33484)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 33484)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33484
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1570_length_33484_cov_11.049478"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="P5516_04745"
     CDS             <1..536
                     /locus_tag="P5516_04745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91780.1"
                     /translation="VVPPQEPWATSPANSSVYRISADGLSSELLFNTQMSFRPMEIAA
                     ISPDLSKLAFLVPYGSLTDNLYTLRLANIDGSGLTDYTTGKINRIPVWSPDNNKFFYR
                     DDASGAWIGQPGSAPVSIPDFNNTRDLVWIDGNRYIGASGPEGGWKLLLGTLGSPTGV
                     IYSSTSGGENLQFTVNR"
     gene            616..1821
                     /locus_tag="P5516_04750"
     CDS             616..1821
                     /locus_tag="P5516_04750"
                     /inference="COORDINATES: protein motif:HMM:NF022702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HRT91781.1"
                     /translation="MKQTNHLWVCVLMALMLLLTACAGGTAPTATQISLEEAVKQTIA
                     AMPTATNTPTPTATPQPTATPTQSIVRYGPDNFPPNVDPLTGLEVADPSILDRRPMMI
                     KVSNFPREGRPHAGLSAADIVFDYYTGEGGNRYLALFYGQDSNQIGPIRSGRLIDRYL
                     VGMYQGVLGAVYAFPSTWETILGYLGWSRVISEGTNTCPAMCRQENVRAEISVFADSA
                     EMTKYYAARPNASNTRQNLNGMAFNTLPPAGGVEARELTHQFGKNNYAGWKYNPETKK
                     YMRWIDSIKENGDVEMVPLTDRNTGEQLQFSNVLLIYAEIETLHHDDTLHEIHILNAN
                     GRAVVFRDGLKYEATYKSGWDTPIQFFNQDGSPFELQPGNTWMHFTGLHSELTEDPSG
                     VWLSRNWKP"
     gene            complement(2196..3029)
                     /locus_tag="P5516_04755"
     CDS             complement(2196..3029)
                     /locus_tag="P5516_04755"
                     /inference="COORDINATES: protein motif:HMM:NF017536.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HRT91782.1"
                     /translation="MREVVEIIEPQWVIEGAGVRLRRSIGSSSFDHLDPFLLLDHFGS
                     TEPADYLRGFPMHPHRGIETVTYMLAGLVRHKDSLGNKGEITSGDVQWMTAGRAILHE
                     EMPEDLGGQMEGFQLWVNLPAKLKMNPPRYQEIKAGKIPWLNPEKGVAVRLIAGKYAN
                     TTGPVTEIAVEPLYMDVAVDSGNSFTYQLTENHNAFLYVFRGEGEFCGVRTQATKLLV
                     LGKGDSVSVKAGLLGTRFLLVAGKPLEEPIVRYGPIVMNTVEEIKQTLREIRDGTFIQ
                     R"
     gene            complement(3116..5038)
                     /gene="gyrB"
                     /locus_tag="P5516_04760"
     CDS             complement(3116..5038)
                     /gene="gyrB"
                     /locus_tag="P5516_04760"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009330 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) complex [Evidence
                     IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HRT91783.1"
                     /translation="MNNHNPTNYGAKDIQVLEGLEAVRRRPGMYVGGTDFKALHHLVY
                     EVVDNSIDEALMGACNRIDIIIHPDSSVTVQDDGRGIPVDIHPEKQKSALEVVMTVLH
                     AGGKFGHSSYKVSGGLHGVGVSAVNALSEWCEVHVYRDGHEYFQRYERGIPQEAVKII
                     SKVPAHKTGTSTTFKFDREIFKGDVDYRFDTLAQRFREMAFVTRNVKIKLVDERPPEK
                     ETTFLFEGGIVSFVRYINRSRDVLHPVYYVEKEIDNIGIEIAVQFTDAYNESVFSFAN
                     TINTIDGGTHLTGLRTAITRVINDYARRANLLKDADANFTGDDTREGMTAIVSIKHPD
                     PQFESQTKVKLMNPEVQTYVQQVVGESFGAFLEENPSAGKAIVNKCLTSARARDAARK
                     ARDLVIRKSALESLTLPGKLADCSDKDSNRTEMYIVEGNSAGGSAKQGRDRHFQAILP
                     LRGKILNTERARLDKILGNEEIKSIISALGTGIGDNFDIKNLRYGKVIIMTDADVDGS
                     HIRTLLLTFFFRYMPQIIEEGHLYIAQPPLYRIAHKNQVQYTYSDAEKEEYLKSLGAQ
                     AEKATLQRYKGLGEMNPTQLWETTMNPESRTLLQVSIDDAASADHTFDMLMGSEVPPR
                     TRFISTHAHAVRNLDI"
     gene            complement(5025..6053)
                     /locus_tag="P5516_04765"
     CDS             complement(5025..6053)
                     /locus_tag="P5516_04765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91784.1"
                     /translation="MNTNEPMLVAAPPRIFPTILKGFNTVAGQVHLILLPVLLDLFLW
                     LGPRLRIYDLFAPAIQMLNANMLKVAPAEMLETVRNATALYTEMFQQFNLFSAIRTLP
                     VGVPSLVARMSVAQTPAVFFGNFEISSLRVGIATLGGLLTLGFLLGAIYFNALSRYSQ
                     PEVNAFNWNKLFNQYAQTLVFFLILVALLIAISIPLLLLVSILSLISAGLAQFIIVLV
                     GFLAIWLILPLVFSPHGIFALDQKVVPSMLLSLRMVRFFLPGTSFFILVCILINEGLN
                     MVWTIPGTASWLLLIGIGGHAFIVTALLAASFLYYRDGIKWMQYNVQKMTETLKKQES
                     GGTQDEQP"
     gene            6140..6958
                     /locus_tag="P5516_04770"
     CDS             6140..6958
                     /locus_tag="P5516_04770"
                     /inference="COORDINATES: protein motif:HMM:NF040134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91785.1"
                     /translation="MSGQKHLPDVERLSVVIALILLAYASTALISLPARSINLQLPGI
                     LLVVKVDFSTLVALVAALLAAAGVEWVLGVHPSLSVHQHWRHWHHWLVPAFSAVAIGI
                     TLGTLPVGAAWWIVFGLGGFLLAAVLTIEYISADPADARYSFAVLGLDAASFALFLII
                     ITAIAGADLRLYILLAAIVPVVFLISARSLFLRLHGSWRLGWAAGITLIIAQLAAALF
                     YLPLRPLQYGLLLLGLLYGLISLASNKEEKANPRTIWIEPTVVLLVFSLLGFLL"
     gene            complement(6983..7831)
                     /locus_tag="P5516_04775"
     CDS             complement(6983..7831)
                     /locus_tag="P5516_04775"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT91786.1"
                     /translation="MHRERVSDNVYWFQSEVYAQVTAGAVTGPNWAVVIDTLALPEEA
                     LEMRTFIEDNLGVPVRYVINTHYHADHSWGNCFFPGATVIAHSIARETMLQKGEPSLE
                     SIKKQNIAFKQVKLVLPHLTFSEGSVTLRVGKKSLVMFATPGHSADGISVLVEEDRVL
                     FAGDAFMPLPYMVDGDPEEISATIKRIGKMGLENIIQGHGDIILRGEIEEATRSNLAY
                     INAVRKAVRAAAKKRDPIEALAEIDIESCGKSRVTLGGLGVELHKRNVVYLYKTLAAE
                     ETNADQ"
     assembly_gap    7877..7976
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7977..8950)
                     /locus_tag="P5516_04780"
     CDS             complement(<7977..8950)
                     /locus_tag="P5516_04780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91787.1"
                     /translation="MTINRTVKAVCYILCAMFIASCSLLTSLSPSVHTDLTPGIDEPT
                     PVNNQAGLSPEENATQNDQDLVHLWAENSTLYPLEVYGVAVGAPEDTLGCGSEFLSGY
                     EEPEMEVTINLNYAQALIPEKVNLVLKEMNEGITRVEILNTRTGLGREIFRENLHRSF
                     SRLENQACALRLELPVEIDFEVNTVIIGFKNLAAAALLDAVELVGSPLSYEEPPVAWR
                     VALNGKPLSLTVDPLNQVWVSLEPNIILRYDVEGNLLEELPAIPSGHITDMTINTDGD
                     LVLNDVDFGEYIILSKGVEVARGGGDAPTLAVAVEPGKKNIYMLGSLDG"
     gene            9210..10826
                     /locus_tag="P5516_04785"
     CDS             9210..10826
                     /locus_tag="P5516_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HRT91788.1"
                     /translation="MEQSHLWWRDGVIYQIYPRSFADSNGDGIGDLIGITQRLDYLVE
                     LGVDAIWLSPINPSPDVDFGYDVADYRNIDPKHGTLADFDHLVKAAHERGLRIILDLV
                     LNHTSDQHPWFLESRSSLDNPRRDWYIWRGSPKGKREAPNQWQSVFGGKAWEFDEVTG
                     QYYYHMFCPEQPDLNWRNPEVYREMLDIFRFWAERGVDGFRLDVFNQYFVDAQFRDNP
                     RKCGIRPFDCQHHLYDTNQPEIQQVVADIRAVLDRYPERYAVGETFLPTTEMASSYSG
                     PGKLHATFDFTMLKSPWNPARYLQCVLRQEELLPEGGWPNYVLNNHDNSRTASRLRAG
                     RGDEKLKVSAAMLLTLRGTPFLYQGEEIGMRDIQVSRAELQDPVGKRYWPLPVGRDGF
                     RSPMQWNGEANAGFTTGKPWLKLHPGYKTRNVESQRKDPASLFNFYKSLLRLRRMYAA
                     LQRGMFLPLHHNPQRVLAYLRQDAAQTILVALNFARRRSKLALGGEITRHQWEMLLSN
                     KEKSQPSIDKGWLQLDGHEVCILLQKPGSG"
     gene            complement(10823..11902)
                     /locus_tag="P5516_04790"
     CDS             complement(10823..11902)
                     /locus_tag="P5516_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ldh family oxidoreductase"
                     /protein_id="HRT91789.1"
                     /translation="MRYYQIEDLKEFASRYFTRWGVSEEDAEITADVLVSADARGVDS
                     HGLIRLQNYYGSRLESGLTNPRAALTTLKETPTTLSLDAHNGLGHPAGVKAMRMCIEK
                     ARNAGLAMATVRNSNHYGIAGYYAMMPLSEGMIGISLTNSAPLVAPTHGRKAMLGTNP
                     IAVAVPAGGARPYVLDMATSIVPIGRITVYQKEGRSIPAGWGINSEGDVTEDPSRVVE
                     GGALMPLGGPELLRGYKGYGLALLVDIFAGVLAGAGFGERVLGPAVSGAANTGHFFAA
                     LRIDAFRDPADFAADMNTLLGALRDSPKAAGEERIYIHGEKEFEKADRALVEGVALSE
                     VAVKSLIQAGKQAGIPFDLVPLRID"
     gene            complement(12123..14054)
                     /locus_tag="P5516_04795"
     CDS             complement(12123..14054)
                     /locus_tag="P5516_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRT91790.1"
                     /translation="MTTSSEKFHGRKAFTPTPLKRRTPVPSDIEIAQEATLKPIMLIA
                     EELGLLPAETEPYGDTKAKVRLEVLQRLKHIPDGKYIDVTAITPTPLGEGKSTTMVGL
                     SQALGAHLGKSVITCIRQPSQGPTFGIKGGAAGGGYSQVVPMEEFNLHLTGDIHAITA
                     ANNLLAAAIDARMFHEKEATDEQLFNRLLPKDKNGKRHFTRGMVARLEKLGISVSDPD
                     ELNEEQRRQLCRLDIDPDTITWRRVVDTSDRFLRGITIGEGPDEKGFTRRTGYDITVA
                     SEIMAILALTTSLKDMRERFGRIVIGISHGGDAVTAEDLGVAGAMTVLMRDAIKPTLM
                     QTVEGTPVFVHAGPFANIAHGNSSILADMIALKLADYVVTESGFGADMGMEKFMDIKC
                     RYSGLIPSVVVLVATIRALKMHGGGPKVVAGKPLDEAYTSENLELLKAGLPNMLHHIK
                     IARKYGIPVVVAVNRFVNDSPAELEMVRKSAVEEGGAEDAVVCEHWEKGGEGAVDLAR
                     AVIRAAEKPAKFEFLYPLDWSIKQKIECITREIYGAEGVDYLPEAEERIAEYTRLGFD
                     KLPICMAKTHLSLSHDATLKGVPKGVRIPIRDIRASVGAGFLYPLLGTMSTMPGLPTR
                     PIFMEIDIEPETGRIIGLS"
     gene            14248..14394
                     /locus_tag="P5516_04800"
     CDS             14248..14394
                     /locus_tag="P5516_04800"
                     /inference="COORDINATES: protein motif:HMM:NF039193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5679 domain-containing protein"
                     /protein_id="HRT91791.1"
                     /translation="MAVIGYCMKCKKKTEMKDTVEVTMKNERKAMKGKCSVCGTGMYK
                     ILGK"
     gene            14623..16581
                     /locus_tag="P5516_04805"
     CDS             14623..16581
                     /locus_tag="P5516_04805"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRT91792.1"
                     /translation="MLPIEEPVVFFLVIMAIILVMPLLSERVHLPGIVGVIIGGMLIG
                     PHGFNILAANDRMEFLATIGLIYLMFSAGLEVDFHQFMRIKGKAAIFGLMTFVIPQVF
                     GMALGWMIHMPLLGCVLLGSAFASHTLLAFPILTRLGVTKNEAVAVTAGATILTDIGA
                     FIILAIVLGSNGGQLELGYFIKIFVLLVAFALATLFILPRLGKWFFQKVKGRTVEFQF
                     VIVLLFIAAMGAKLIGVHEVVGAFLAGLAVNATLPRHSPVTSHVLFMGESFFIPVFLL
                     FSGMVTDPLTLLHTPGALFLTLGILVVAYLSKFLASLVTSRLFKYSKEEFLTVFGLSH
                     AQAAVTIPTLVIGMQLNLFSTELFNAAMMMILLTSITSPLIVQKFAPGLHQEVTELEE
                     LPLFQRVLVPIANPGTQEHLLALAGLLAKSGGGEVIALNVVLDRNDQGANLSQQKELL
                     NRVPELLKDPETRYELVPRLADSYARGILQVSRERGSTLIVMGWRGKRTFRTSVLGTV
                     LDEVIWGSDTPVMVGKLSRSLNGTRRVLLLIPAGVVSPSALRRVVQANMTLARELNVP
                     LEVLAAKSYLELIKRIIGKNPNQIAITVQEMVGALRLKDLEKNVESDLLVIPGFGSRK
                     HFIASLGNLPERLADSFSGNLIILHFDR"
     gene            complement(16622..18580)
                     /locus_tag="P5516_04810"
     CDS             complement(16622..18580)
                     /locus_tag="P5516_04810"
                     /inference="COORDINATES: protein motif:HMM:NF013080.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HRT91793.1"
                     /translation="MPTLKKNSSIKELLRPVMRPAWSPTSLMVLTSAIAALYILMEWI
                     FLFTRPSFLQTIAFLQKFLALSTASGLLVLACGLALLPFLASGQVFQQAAFRKMLTLT
                     AMLLPAFLLAALALLLLDNFTYTIFQLGIVSTRDWIRGLYGVLFSFLVVYLFFKLVAF
                     HHLLAAFFSRWKPAQRLMLVGSIFLLSLASAGIPALSMGNQLLGSSRFHPAGSRSIPP
                     DILLITVDGVNAEYTSLDAYERDTTPFLRAISAESLRARNAFANAQGTIGSITTLLTG
                     KYPMDTRVIYSSDLLQGEDAYQHLPEILEDFGYYTVQLSNPVYADAYKTNFQNAFNEA
                     NGRSADQQPLAFALARIYPGINYLFQQEMTDRLSDRLGHIFFLSDMSNPYEQVTEAAV
                     KFNDWEKIDYLFRLLEESRQPVFAHLHWMGTHGPRYFPDKQVFSAGISVDEQGNNNQL
                     FYYDAILEFDQAFEEIYARLEKSNQLENTLLVVTSDHSQKWTNSRLPLLIRFPRAERQ
                     GVLDQNVSTVDIAPTLLDYLGIKTPKWMAGQSLLSPSYIERPIFTVRIPKSSKDPVTG
                     KVIYPESEPPFFQFGRISVIVCDQWYDLDLVEMSMTHGNITAYATPCSAQINESQALE
                     LIMQHLQRYDFDTDTLLPLRARLEGEHP"
     gene            complement(18845..18928)
                     /locus_tag="P5516_04815"
     tRNA            complement(18845..18928)
                     /locus_tag="P5516_04815"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(18892..18894),aa:Leu,seq:taa)
     gene            complement(19013..19657)
                     /locus_tag="P5516_04820"
     CDS             complement(19013..19657)
                     /locus_tag="P5516_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HRT91794.1"
                     /translation="MLKVDFHTHTRYSEDSLTPIHELISAARKRGLDRVVVTDHNVLT
                     GALEAWRAAPDLVIPGEEVQTSEGELLAAFVTVVVPRGLEPLEALHRLRKQGAFISIS
                     HPFDPQRSGWSRETLDLIASQVDAIETGNGRVLTPAYNQRAAEYAASLGLPGTAGSDS
                     HHPSEVGRMYTLVPEFNDAESLRLAMRSARVCGEPSSPLVHLYSVQAKAVKRWR"
     gene            complement(19644..20633)
                     /locus_tag="P5516_04825"
     CDS             complement(19644..20633)
                     /locus_tag="P5516_04825"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HRT91795.1"
                     /translation="MITDTRQNYRVLLPGLLLGLAVFALLISLSSIHQVFTLFESFKW
                     VYFAVAVGLNIIHHALRFLNLSFSLNISGIRRIPLKKRMAFYLSSSALTAADPKAQES
                     YRSVWLSKACGIPLVRIDSIFLIDRLSDGLSILALATLGILAYPAFWPFFAAMLLLFV
                     LSILFLQIKPAGEGLLDAGEKLPLLRLFSRQLRQCMEGNEHLFRTFPMTVSLFFNVLA
                     WIALAAALFFVLVGFGLEPSLTLAAIACLVLAFSIIIGFFSTVPGGLGVVELALAGLL
                     TVLLGFQPELAVTATILFRMSTYWISLLFGILFWSAAAKPCTAEPGVSGFAES"
     gene            20956..22680
                     /locus_tag="P5516_04830"
     CDS             20956..22680
                     /locus_tag="P5516_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HRT91796.1"
                     /translation="MTQNTRVFGTSQNSRKPLTWGDIIALSLVIALLYVGARLAVNAP
                     EVVPGPDISLSPSALPIYTLFSVGRMAAAYLLSFLFSIIYGRVAAYHQNAEKILMPLL
                     DVLQSVPILSFLPVVLLSLSSFLPEGTAAEIASIVLIFTSQAWNITFAWYQSLTTIPK
                     ELKEASSIFRLNKWINFKALELPFGMISLIWNSMMSWAGGWFFLMAAEIFTVGSRDFR
                     LPGLGAYLQEAANQSNHVAIFLGTGVLIFTVVLLDQLIWRPLIAWSDRFKVEMVENDN
                     PPTSWFLDLMHNANITRIISNRIFAPLTEWFDRLTIKHAPPVSRLENGKNQLKWYVIV
                     FSIIVFALISYGLYHATEMLLSVPVQKWGVIGLGVTATFLRVIVALLIALAWTIPVGV
                     LIGTNRPLATVLQPIVQVTASVPATALFPVLLLLFLNAQGGLNTAAVLLMLMGTQWYL
                     LFNVIAGASAIPQDLIFTSKLMGLSGWQRWRTLLLPAIFPYLVTGAITASGGAWNASI
                     VAEYQKFGEKTHTVTGIGSLIAQATASGDYPLLLAGTLAMIITVVVVNRVIWRRLYRL
                     AEEKYRME"
     gene            22683..23972
                     /locus_tag="P5516_04835"
     CDS             22683..23972
                     /locus_tag="P5516_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitrate/sulfonate/bicarbonate ABC transporter
                     ATP-binding protein"
                     /protein_id="HRT91797.1"
                     /translation="MTEALLQLEHVFQSYGAGVRRFTAVENVNLNLSVGEFVALLGPS
                     GCGKSTLLRIIAGLQKATQGKVLYRGQQLEGVNPHTSIIFQTFALFPWLTVLENVSLA
                     LKAKGVEKHERDKKAEDLLDRVGLDGFESAYPRELSGGMRQKVGFARAMAVEPELLCM
                     DEPFSALDVLSAESLRGELLELWTEGKIPTKAILMVSHNIEEAVFLADRIVVMDKEPG
                     RIIAEHKIDLPHPRNRKSVEFQNHVDQVYAVLAGQTRPEKEELGTAPGEVGITRHLPE
                     VEISDLAGLLENLAEGGNQKHDIYLLEDKLGITSDQLLKLTEAAELLGFSSIAKGDIE
                     LTALGQAFADASILARKEIFASCVRRIPLMRWLLSMLHSAEDRRLKIEVILTALSLDF
                     STEEAEKQIEVLIKWGRYGELFVYDDDQEMIELEEGR"
     gene            24338..26251
                     /gene="nusA"
                     /locus_tag="P5516_04840"
     CDS             24338..26251
                     /gene="nusA"
                     /locus_tag="P5516_04840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HRT91798.1"
                     /translation="MKNEFTLAFNEVLEDKGLPKEVILEALESAMVSAYRKSVNASNE
                     QPIEAKIDLETGKVTIYAQKEVVEVVENNITEVELETARKVEPEAQLGDLVVVESTPG
                     DFGRVAATTARQVIQQRIRDAERKAQISYYEKQLGEIVSGVVQAIGAAGLTIGLELKT
                     EATMPRKEMITNEHFHIHERVRALVADVKDSPRGPQIILSRTHRDFLRRLLENEVPEI
                     FHGIVEIRSIAREPGDRAKVAVSATQQGIDPVGACVGIRGVRIQAIVKELHDEKIDVI
                     EWNPDPAVFIAKAISPARVSGVYLTDQHSGNAKTATVVVPEDQLSLAIGRIGQNARLA
                     AKLTGWRIDIKSLPEAAADALQKLATDPAVEALKESEHDNALRIEELLARKEEGKPLS
                     PEEYDQMAKFVDRVERKSIAKSEEQRKQELEKLAEIKKQIPASAFELNILDSGLPEHV
                     AVILQEAGYFSVGDLAMQVKQDPDLILGINGIGIRAMESIHELMAALDKQLADEQAAL
                     VKQAEETAEEMEVLEPVSEIELPAEVDVVPSVEVEPVLDVTVGETSSTETIEEPEPEK
                     EPSLEELFTLRPEVLDTAGLLDDDEAEESGKAKKKGKKKGKSVVVTYDPDRDLTVVQK
                     KHKRGGNDWDWDE"
     gene            26269..26598
                     /locus_tag="P5516_04845"
     CDS             26269..26598
                     /locus_tag="P5516_04845"
                     /inference="COORDINATES: protein motif:HMM:NF016204.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HRT91799.1"
                     /translation="MAKKPQKRIKHLPQRTCIGCKTVLAKQELVRLVRTPEGIMVDET
                     GKAAGRGAYVHNTRRCWEQALKGPISHALRTDITPADRQRLKEYLERLPEVDPIENRA
                     ESRDVTN"
     gene            26702..28477
                     /gene="infB"
                     /locus_tag="P5516_04850"
     CDS             26702..28477
                     /gene="infB"
                     /locus_tag="P5516_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HRT91800.1"
                     /translation="MSEKTIEFPSQITVRDLAQRMEASPIQVIKILMSNGVMANINQQ
                     VDFDTAAVVASELGFEAVLESVKETAPAEDPGKVPLWRELIDSEDKSKLINRPPVVTI
                     LGHVDHGKTTLLDAIRHANVASGEAGGITQHIGAYQVEHKGKVITFLDTPGHAAFSAM
                     RARGAQGADIVILVVAADDGVMPQTREAIAHAKAARVPIIVALNKIDRADANPDFVKR
                     QLSEQGLVPDEWDGNTIVVPVSAKQKKGLDDILEAVLLVSESLDIKANPHGKVFGTVI
                     EAQVDKARGVIATLLVQNGTLQTGNTVIVGDTCGKIRAMYDFNGKTVKSAFPSTPIQV
                     LGIGNVPPAGELFQVVPSEKEARLILAERAASKENGSETSTRTSLEKLFEKFQAGEVR
                     ELPLILKVDVQGSVEPIVNSLNELSQGEIKINVIHAETGNISESDVMLAAASRAILLG
                     FNVSADQAARNLAEQEGISIRLYNIIYRMIEDIEKALKGMLEPETREIPVGTAQVRAV
                     FKISKVGTIAGCRVLTGELRRNARIRLLREGETLFDGEISSLKHEKDDVKEVRQGFEC
                     GVSLKGYNDINEGDLLENYIIEKSA"
     gene            28503..28874
                     /gene="rbfA"
                     /locus_tag="P5516_04855"
     CDS             28503..28874
                     /gene="rbfA"
                     /locus_tag="P5516_04855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HRT91801.1"
                     /translation="MPSPLRLTRIGDRFRKELSEMLVKEEIRDPRLAGISITDVRVDR
                     ELSYADIYVSAVEGASRSKEVLEGLESASGFIRKALSDRIQLRSFPRLRFHWDITPER
                     ADRIEQLLASIREEERQQKKK"
     gene            28871..29872
                     /locus_tag="P5516_04860"
     CDS             28871..29872
                     /locus_tag="P5516_04860"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRT91802.1"
                     /translation="MIDRQIKELLHRSKKIGLTSHIRPDGDAIGAVLGLGLALTAAGK
                     RVEMVLRNGISKTFRHLPGAERIQSAFKTENDLYIVLDCSDLERTGGVLNGREVDIVI
                     DHHITNKGFGKVNLIEPESVATCATLAQHMPDWGLVIDKAVASALLSGIISDSIGFRT
                     SNTTALSLRIAADLIEKGADINQLYNRALISRPFTSVHYWGYGLAKLERENGLVWTTL
                     SLADRHNAGYQEDDDADLTNILSSIEDMDVVVLFIEQKPDQVKVSWRARPGINVASIA
                     DSLGGGGHPAAAGVEMEGNLAQVKDLILSKTRDYLRSLKPENGSPRDSEVTEHINGK"
     gene            29862..30773
                     /gene="truB"
                     /locus_tag="P5516_04865"
     CDS             29862..30773
                     /gene="truB"
                     /locus_tag="P5516_04865"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HRT91803.1"
                     /translation="MENNSGIDVKNAISGVLVVDKPVGMTSHDVVQIIRKGSTIKRAG
                     HTGTLDPRASGVLVVLLGPAVRLSEYVSASDKRYQAVIMLGTTTDTYDADGRTTSSAP
                     VNISEEEFNNELQKFVGQIEQVPPPYSAVKVHGQKAYDIARRGEEVELAPRIIHVYSL
                     ELLEWAPPEVVIDVYCSSGTYVRSLVNDLGSRLGCGATLSGLRRTKSGRFTLRDAVPL
                     RKLNEAFADGSWYQYLIPAAEALSDWPMIELNDQEVDSIKHGIRIPAVDAKQQKVCGV
                     SGQGELVALLELDAEKMEWQPKKVFFS"
     gene            30848..31777
                     /locus_tag="P5516_04870"
     CDS             30848..31777
                     /locus_tag="P5516_04870"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: protein motif:HMM:NF004162.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HRT91804.1"
                     /translation="MNYIEALQQANLKESWATIGAFDGVHIGHRTLFLKLVDGARQDG
                     CKAVAITFEPLPALFFNRIKNGQVLTTLAQRVSLIKALGIDEVIVLDFDQALADVEAF
                     PFLQQVHKAIGLRRLLSGFNSRIGKDQAGAVPKLQEIGQVLGFCVEVVPPVKNGQEII
                     SSSNIRKLLKAGEITRANQFLGRPYALIGEVVHGEHRGSKLGIPTANLRLPAEQMLPA
                     SGVYACRASVGELAFIAVTNVGIRPTFENPLPVPRVEPHLLDTSETFYGVNLELEFFA
                     FLRPEIKFPDAKALVAQIQLDIQKTREYFANAA"
     gene            31767..33197
                     /locus_tag="P5516_04875"
     CDS             31767..33197
                     /locus_tag="P5516_04875"
                     /EC_number="2.1.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF014560.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050797 - thymidylate synthase (FAD)
                     activity [Evidence IEA];
                     GO_process: GO:0006231 - dTMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent thymidylate synthase"
                     /protein_id="HRT91805.1"
                     /translation="MQPKRQIYLLDPQQLSPETIAVTFAKTSRSPESFRDIAAELTAA
                     SSADFNQKWVVGYGHSSVAEHAVLHIALENVSRLAVENIESNRLASYTEKSTRYQTWE
                     DDAFHLPAELTGHPLEGLFTSACATLFSIYRSCIQAVIAHLKSNSQSQAGESEAQLNR
                     RLRSQAIDACRFLLPAASLANVGVTINARALEHAVGKLLTSPLIEAQQIGAEIRHAAS
                     ASIPTLLKYADQIPYLQHVCQQFSALSIQVDAAPEPDWCQLIGPQPDVLEKILAAELY
                     REQDMSFTQALKAIRTLPDHQKNTLLRCLLETPDRHTIPSRELEYAFFTFDLLIDQGA
                     YFEVKRHRMMTQTAQLLTARFGYAIPALITEAGMDAAYCAAMEKAREVYDQLADFNPD
                     VAAYIVPNGFNRRVLLHLNLRSLVHFIRLRSAPNAHFSVRRVAQRMAEEISAHFPELS
                     PLLGRNENETLKNIETTFFTNTHVVS"
     gene            33213..>33484
                     /locus_tag="P5516_04880"
     CDS             33213..>33484
                     /locus_tag="P5516_04880"
                     /inference="COORDINATES: protein motif:HMM:NF014373.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HRT91806.1"
                     /translation="MFIPETEILIRLGLAALLGMMVGYERERQNQPAGLRTHVILAVG
                     SCLAMTVSIDLAIQFYPHAPNGDPARLAAQVISGIGFLGAGAILRYG"
BASE COUNT         8017 a         8830 c         8775 g         7762 t
ORIGIN      
        1 cagtcgttcc gccccaggag ccctgggcaa cctcgcctgc caattcgagc gtgtaccgca
       61 tttcggcgga tggactgtca agcgaattgc tttttaacac ccaaatgagt ttcagaccta
      121 tggaaatcgc cgccatctct cctgaccttt ccaaactggc tttcctggtg ccttatgggt
      181 cgctaaccga taacctgtat accctgcgcc tggccaatat cgatggcagc ggtttgaccg
      241 attacaccac tggcaagatt aacaggattc cagtatggtc gcccgacaat aacaagttct
      301 tctatcgcga tgatgccagc ggcgcctgga tcggtcaacc gggttccgcg cctgtatcta
      361 tacccgactt caataatact cgcgacctgg tctggattga tggcaaccgc tatattggcg
      421 catcgggtcc tgaaggcgga tggaaactcc tgctcggaac acttggctct ccaactgggg
      481 tgatctattc atccacgtct ggcggtgaaa atcttcagtt cactgtcaac cgctaaactc
      541 ctttagaagt tggaacgtta gcgagctggg ttataatttc tgggtattga tcaacctgga
      601 aaactcggag attctatgaa acagaccaac catctgtggg tttgcgttct tatggcgctg
      661 atgttattgc tcaccgcctg cgcaggcggc acggcgccaa cggcgactca aatcagcctg
      721 gaagaagccg tgaagcagac catcgccgcg atgcccactg caaccaacac cccaacgccc
      781 acagccactc ctcagcccac tgctacccct acacagtcca ttgtgcgtta tggacctgat
      841 aacttcccac caaatgtaga ccctctcacc ggtcttgagg tagctgaccc ctctatcctt
      901 gatcgtcgac cgatgatgat caaggtttcc aacttcccgc gtgaaggtcg cccgcacgcc
      961 ggtttgtctg cggcggatat tgtatttgat tattacactg gcgagggcgg aaatcgttat
     1021 ctggcgttat tttacggaca agattccaat cagatcgggc ccattcgttc cggcaggctg
     1081 atagatcgtt acctggtggg aatgtatcaa ggtgtgttgg gcgctgttta cgcctttccc
     1141 tccacctggg aaaccatcct gggttacctg ggttggagca gggttatctc tgaaggcacc
     1201 aatacctgcc cggcaatgtg ccgccaggaa aatgtgcgag cggagataag tgtattcgcc
     1261 gattccgcgg agatgaccaa gtattacgcc gcccgcccca acgccagcaa cacacgccaa
     1321 aaccttaacg gtatggcgtt taacaccctg ccgccagccg gcggcgttga ggcgcgggaa
     1381 ctcactcacc agtttggcaa aaacaactac gcgggctgga agtacaatcc tgaaaccaaa
     1441 aaatatatgc gttggattga cagtatcaag gagaatgggg atgtagaaat ggtgccgttg
     1501 accgaccgga acacaggtga acagctgcaa ttctccaacg tcctgttaat ctacgccgag
     1561 atcgaaaccc tgcaccacga tgatacgttg cacgagattc atattctcaa tgccaacggg
     1621 cgcgctgtgg tctttcgcga cggattaaaa tacgaagcaa cctacaaatc cggttgggac
     1681 acccctatcc agttcttcaa tcaggacggt agcccctttg agctgcaacc ggggaatacc
     1741 tggatgcatt tcaccggcct gcactcagaa ctcaccgaag acccctccgg ggtttggctc
     1801 tcgagaaact ggaagccgtg atcgaaaatg atactgccag tcgaggttgc cctgcttgcg
     1861 gggcaacctc atttctttgc ctggattcca ggtgaactgc gcgcggcttc acactggtaa
     1921 acagaatcta tccttcatca tacccacgac tgtcgccagg tcttccctgt gcgccctgcg
     1981 cgccttcaac ccctccatgc tttcgtggct tcctcctcaa tggagacctc ccaacgatgc
     2041 cgataagaag aagggcagtg tcctgaacct cgcgattcga cccagcttag cccaatgctc
     2101 ttttcggaaa cgttttgcgc gaaggaacca cgttcagccc aatgaaggct tgaaaagtga
     2161 aaaatacccc catatcattt ttcaactccg caggcttagc gctggataaa agtcccgtcg
     2221 cgtatctccc gcaaggtttg cttgatttcc tcgactgtgt tcatcacaat tggcccataa
     2281 cgcacgatag gctcttccag aggtttacca gccaccagca ggaaccgggt tcccagcaac
     2341 ccggctttca ctgaaacgct atctcctttg cccaacacca gcagttttgt cgcctgggta
     2401 cgcaccccgc aaaactcacc ttcacctctg aacacgtaaa gaaaagcgtt gtggttctcc
     2461 gtcagttgat aggtaaagga attcccgctg tctaccgcca catccatata aaggggttca
     2521 accgcgatct cagtaactgg gcctgtggtg ttggcatatt ttccagcgat cagtcgaaca
     2581 gccacgccct tttcagggtt cagccagggg atcttcccag ccttgatttc ctggtatcgc
     2641 ggcgggttca ttttcagctt cgccggaaga ttgacccata actgaaaccc ttccatctgc
     2701 ccccccaggt cctccggcat ctcttcatgc aatatggcgc gccccgcggt catccactgt
     2761 acatcacccg aggtgatttc gcccttgttt cccaggctgt ctttatgcct taccaggcca
     2821 gccagcatgt aagtgaccgt ttcaattcca cggtgcggat gcatgggaaa accgcgcaaa
     2881 tagtcagccg gttcagtcga accaaaatgg tccagcagca aaaaaggatc caggtggtcg
     2941 aaggaggaag acccgatgga gcgacgcagc ctgacgcccg cgccttcgat cacccattga
     3001 ggttcaatga tttccaccac ttctctcatc aattgctcct gcctttcttc tttgaccacc
     3061 tttcattcgt ctgtaataaa aggctcccgg tgtttgtctg tcgggagcca ggttatcaga
     3121 tatccaggtt gcgcacagcg tgcgcgtgcg ttgagataaa gcgcgtgcgc ggagggacct
     3181 ccgaacccat cagcatatca aatgtgtggt ctgctgacgc ggcatcatcg attgaaacct
     3241 gcagcaaggt gcgcgattcc gggttcatcg tggtttccca caactgggtg gggttcattt
     3301 cacccagtcc tttgtaacgc tgtaacgtgg ccttttccgc ctgcgctccc aaagatttca
     3361 ggtattcttc tttttcagca tcgctgtagg tgtactgcac ctggttcttg tgcgcgatgc
     3421 ggtatagcgg cggttgcgcg atgtacagat gcccttcttc gatgatttgc ggcatatagc
     3481 ggaagaagaa tgtcagcaac agggtgcgga tatggctgcc gtcaacatcc gcgtcggtca
     3541 tgatgatcac cttgccataa cgcagatttt tgatatcaaa attatcacca attccggtgc
     3601 ccaacgccga gatgatcgac ttgatctctt cgttgcccag tatcttatcc aggcgcgcgc
     3661 gctcggtgtt gaggatctta ccgcgcagcg gtaagatagc ctggaaatgg cggtctcgcc
     3721 cctgcttggc tgaaccgccg gctgaattgc cttccacgat gtacatctcc gtgcgattgc
     3781 tatccttatc agagcaatct gccagcttgc ctgggagggt gagcgattcc agcgccgatt
     3841 tacggatgac caggtcacgc gctttgcgcg cggcatcgcg ggcgcgagcg gaggtcaggc
     3901 atttgttcac aatcgccttg cccgcggagg gattttcttc cagaaaagcg ccgaaagatt
     3961 ctcccacaac ctgctgcacg taggtttgca cctccgggtt catcagtttg accttggtct
     4021 gactttcaaa ctgagggtca ggatgtttga tgctgacaat cgccgtcatc ccttcacggg
     4081 tatcatcacc ggtgaaattg gcatccgcgt ctttcaacag atttgcgcgg cgggcgtaat
     4141 cattgatcac cctggtaatc gcggtgcgca agccggtgag gtgcgtaccg ccgtcaatgg
     4201 tattaatcgt attggcaaag gaaaaaaccg attcgttgta cgcatcggtg aactgcactg
     4261 cgatttcaat accgatatta tcaatctctt tttccacata gtagaccggg tggagcacat
     4321 cccgggaacg gttgatgtag cgtacgaaag aaacgatgcc gccttcgaaa agaaaagtgg
     4381 tttctttctc cggggggcgc tcgtcgacca gcttaatctt gacgttgcgg gtgacaaaag
     4441 ccatctcgcg gaagcgctgc gccagggtgt cgaagcggta gtccacatct cccttgaaga
     4501 tttcccggtc aaacttgaaa gtggttgagg tgccggtttt atgcgctggc actttgctga
     4561 tgatcttcac cgcctcctgc ggaatcccgc gttcataacg ctgaaaatac tcatgcccat
     4621 cccggtatac atgcacttcg caccattcgg ataaggcgtt cacagccgaa accccaacac
     4681 catgcaaccc gcccgaaacc ttgtacgaag aatgcccaaa cttacccccg gcgtgcagca
     4741 cggtcatcac cacttccagc gcagatttct gtttttcggg gtgaatatcc accgggatgc
     4801 cgcgcccgtc atcttgcaca gtaacactgc tgtcagggtg aatgatgatg tcaatacgat
     4861 tgcaggcgcc catcagggct tcatcaatgg agttgtcgac aacttcgtat acaaggtgat
     4921 gtaaggcttt gaaatcggtg ccgcccacat acatccctgg gcggcggcgc acggcctcaa
     4981 gcccctccag cacctggatg tctttcgccc cgtagttggt ggggttatgg ttgttcatcc
     5041 tgtgttcctc cgctttcctg tttcttaagc gtttctgtca tcttttgaac gttgtattgc
     5101 atccacttaa tcccatcccg ataatacagg aaactcgccg ccagcaaagc ggttacgatg
     5161 aacgcgtgac caccgatccc aatcagcagc agccaagacg cggtacctgg tatcgtccaa
     5221 accatattca gtccttcatt gataaggatg cataccagga tgaaaaaact ggtgccaggg
     5281 agaaagaacc gcaccatcct caggctgagc agcatcgaag gaaccacttt ctgatccagc
     5341 gcgaaaatgc cgtgaggaga aaacaccagc ggcagtatca gccatatcgc caaaaaacca
     5401 accagcacga tgatgaactg cgccagtccg gcgctgatca atgaaagaat ggaaaccaac
     5461 aataacaagg gtatcgaaat tgcgattaac aacgcgacca agatcaaaaa gaacaccagt
     5521 gtctgggcgt actggttgaa tagcttgttc cagttgaatg cgttcacttc cggctgggaa
     5581 taacgggaga gcgcgttaaa gtaaatggcg ccaagaagaa aacccagtgt gagtaatccg
     5641 cccagtgttg caatccccac tcgcagcgat gaaatctcga aatttccaaa aaagacagcc
     5701 ggggtctggg ccacgctcat acgtgccacc aggctgggca caccaactgg cagtgtgcgg
     5761 atggcagaaa acaaattgaa ctgctggaac atctccgtgt acagggcagt tgcgttgcgc
     5821 actgtttcga gcatttctgc aggagccact tttagcatgt tagcgttgag catctgaatt
     5881 gccggcgcga acaggtcgta gatacgcaat cgtgggccca gccacaggaa aaggtccagt
     5941 aaaacgggca ataatatcag gtgcacctgc ccggcaaccg tgttgaaacc ttttaggatt
     6001 gtagggaata tccggggagg ggcggcgaca agcataggtt cgttggtatt catacagtct
     6061 atcattttac catatctgcc cctcttctcc cgctcgtcgc gctccgattg gcgcacagta
     6121 gatggggtaa aatacccttg tgagcggaca gaagcatctc ccggatgttg agcgtttaag
     6181 tgtggtcatc gccttgattt tgctggcgta cgcctccacc gcattaattt cgctacctgc
     6241 gcggtcaatc aatttacagc ttccgggtat tttactggta gtcaaagtcg atttttctac
     6301 acttgtggcg cttgtggcag ccttgctggc ggctgccggg gtggaatggg tgctgggggt
     6361 tcatcccagc ttgagcgttc accaacattg gcggcactgg caccattggc tggtgccggc
     6421 gttcagcgct gtagccattg gcatcacgct cggcacactc ccggtgggag ctgcctggtg
     6481 gatcgtgttc ggtttgggcg ggtttttgct ggcggctgtg ttgaccattg agtatatctc
     6541 cgctgacccg gctgatgcgc gttactcttt cgctgtgctg ggtctcgatg cggctagttt
     6601 cgcgctattc ctgattatca tcactgccat cgctggggct gacctgcgcc tgtatattct
     6661 gctggcagcc atcgtaccgg tggttttcct tatcagcgcc cgttcattat ttttacgcct
     6721 ccatggctcc tggcgcctgg gctgggcagc cggcatcacc ctcattatcg cccagttagc
     6781 ggcggcactg ttctacctcc cgctgcgccc cttgcaatac ggtctgctat tgttaggttt
     6841 gctctatggg cttatcagcc tggcatccaa taaggaagaa aaagccaacc cgcggacgat
     6901 atggatcgaa cccacagtgg tgttgctggt cttttcgctg ttgggttttt tactataaaa
     6961 aaaccggctt ttcgccagcg ggctactggt ccgcgttggt ttcttcagca gccagcgtct
     7021 tgtacaaata gacaacattg cgtttgtgca gttctacacc caaaccgccc agggtgacgc
     7081 ggctcttccc gcaggattca atatcaatct ccgccaatgc ctcgattggg tcgcgttttt
     7141 ttgcagccgc gcgaaccgcc ttacgcaccg cgttgatgta agccaggtta gatcgggtcg
     7201 cttcttcaat ttcaccgcgc aagatgatgt ctccatgccc ctggataata ttttccaaac
     7261 ccatcttgcc aatgcgcttg atcgtggcag aaatctcttc cgggtcgcca tccaccatat
     7321 aaggcagcgg catgaaggcg tctccggcaa agagcaccct gtcttcctcc accagcacgg
     7381 aaatcccatc tgctgagtgc cccggagtgg caaacatcac cagtgacttc ttccccacgc
     7441 gcaaggtcac gctcccttca gagaaggtca gatgcggcag tacaagtttg acctgcttaa
     7501 aggctatatt ctgtttttta atcgattcca gcgatggctc gcctttctgc agcatcgttt
     7561 cgcgggcgat tgaatgcgcg atcaccgtgg cgccggggaa gaagcagttg ccccacgaat
     7621 gatcggcgtg ataatgcgta ttgatcacat agcgtaccgg cacgccgagg ttatcctcga
     7681 tgaaagtgcg catttccagc gcttcttcgg gtaaagccag cgtgtcgatg accaccgccc
     7741 agttaggacc ggtgaccgcc ccggcagtta cctgggcata aacctcgctt tgaaaccaat
     7801 aaacattatc tgatacccgt tctcgatgca tgctctcttc cttaatgcct tcgtagtgct
     7861 aattcaatat tcaattnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnccat
     7981 ccaggctgcc cagcatgtag atatttttct tcccgggttc cacagcaacc gccagggtgg
     8041 gagcgtcgcc accgccgcga gcgacttcaa ctcccttcga aaggatgatg tattcaccaa
     8101 aatccacatc attgagcacc aggtcaccat cagtattaat ggtcatatct gtgatatgac
     8161 cgcttggaat ggcggggagt tcttccagca agttgccttc aacatcatat ctcaggatga
     8221 tgttcggttc gagcgaaacc caaacctggt tcaacgggtc gacggtcagg ctgagcggct
     8281 tgccattaag ggcaacccgc caggcaaccg gtggctcttc gtaagagagc ggagatccca
     8341 ccaattcgac cgcgtccagc aacgctgcgg cagccaggtt tttaaaaccg atgataacgg
     8401 tgttcacctc aaaatcgatt tccactggca gctccaggcg taaggcgcac gcttgatttt
     8461 caaggcggga gaaggagcgg tgcaggtttt cacggaaaat ttccctgcca aggccggtgc
     8521 gcgtgttcag aatttccacc cgtgtgatac cctcgttcat ttctttgagc accagattga
     8581 ccttttccgg gatcaaggcc tgcgcgtagt tcaggttgat cgtgacttcc atctctggtt
     8641 cttcataccc cgacaggaac tcgctgccgc agcccaaagt gtcttcaggc gctccaaccg
     8701 caacgccata cacctccagc gggtagagcg tgctgttttc agcccaaagg tgcaccaggt
     8761 cttggtcatt ttgcgtggcg ttttcttcag ggctgagccc agcctggttg ttcaccggag
     8821 tgggttcgtc aatgcccggt gtcaagtccg tgtgtacaga tggggatagg gatgtcaaca
     8881 acgagcaaga cgcgataaac attgcgcaca ggatgtaaca aactgccttt acagtgcggt
     8941 tgatagtcat ctttactcct tcaacaaaat ttcatcaggt tcccttacta tataatagtt
     9001 catccaggaa aacaaccgcc ttactctggt cttgcgtgat gacgaaccaa cccgcacatc
     9061 aggcaggcag cataagagca tcggctgcct gcgcgagggc tcactatcca taggtcaata
     9121 cgtcgctttc cgcagccggt ctggctggtt cggcgttgtt ggtgttgatt aacggggcta
     9181 tgttaaaatc aactcacaag gagatgcaca tggaacagtc tcatttatgg tggcgggatg
     9241 gcgtgatcta ccagatctac ccgcgttcat tcgcggacag taatggtgat gggattggag
     9301 acctgattgg tataacccaa cgtctggatt acctggttga gttaggcgtg gatgctatct
     9361 ggctttctcc catcaacccc tcgcctgatg tggattttgg ttacgatgtg gcggattatc
     9421 gaaatatcga ccccaaacac ggtacactgg cggattttga ccacctggtg aaagccgcac
     9481 atgaacgagg gttgcgtatc attcttgacc tcgtcttgaa tcacacatcc gaccagcacc
     9541 cctggttcct ggaatcacgc tcctcactcg acaaccccag gcgtgattgg tatatatggc
     9601 gcgggagccc gaagggcaaa cgggaagcgc ccaatcaatg gcagtccgtc tttgggggca
     9661 aggcctggga gtttgatgag gtcaccggtc agtattacta tcacatgttc tgcccagaac
     9721 agcccgacct gaactggcgc aatccagaag tttaccgcga gatgctggat attttccgtt
     9781 tttgggctga gcgcggtgtt gatggcttca ggctggacgt gttcaaccag tatttcgtcg
     9841 acgctcaatt ccgcgacaac ccgcggaaat gcggtatccg cccctttgac tgccaacatc
     9901 acctgtatga taccaaccag ccggaaatac aacaggtggt tgcagatatc cgcgctgtgc
     9961 tggaccggta cccggaacgt tacgcggtag gcgaaacctt tttacccacc actgagatgg
    10021 cttcaagtta tagtggaccg ggcaaactgc acgccacatt cgacttcacc atgttgaaga
    10081 gcccatggaa cccggcgcgc tacttgcagt gcgttctgcg gcaggaagag cttcttccag
    10141 agggcggctg gccaaactat gttttaaata atcacgataa ttccaggact gcctcccgct
    10201 tgagggctgg gcgcggtgat gaaaaactca aagtctcggc ggcgatgctg ctcacactgc
    10261 gcgggacacc gtttctctac cagggtgaag aaatcggcat gcgtgatatc caggtcagcc
    10321 gcgccgagct acaggacccg gtaggaaagc gctactggcc cctgccagta ggcagggatg
    10381 gctttcgttc tcccatgcaa tggaacggag aagcgaacgc aggctttacc accggcaagc
    10441 cctggttgaa gttacacccc gggtataaaa cccgtaatgt tgaatcgcag cggaaagacc
    10501 ccgcgtcttt gttcaacttc tacaaatctc tgctcaggtt acggcgcatg tatgctgcgt
    10561 tgcagcgggg gatgttctta ccgctgcatc acaacccgca gcgggtgctg gcttatctgc
    10621 gccaggatgc cgcacaaacc atcttggtgg cgcttaattt tgccagaagg cgctcaaaac
    10681 tggcgctggg tggtgaaata accaggcatc aatgggagat gctgctttcg aacaaagaaa
    10741 aaagccagcc ttcgattgat aaaggctggc ttcaattgga tggacatgag gtttgtatct
    10801 tgctgcaaaa gccagggtcg ggttagtcaa tcctaagcgg cacaaggtca aaaggaatgc
    10861 ccgcctgttt tccggcctga ataagcgatt tcaccgctac ctcagaaagc gccactcctt
    10921 caacaagtgc gcgatctgct ttttcaaatt ctttctcccc gtggatatag atgcgctctt
    10981 cgccggcggc tttcggggaa tctcgcagcg caccaagcag cgtgttcata tcagcggcaa
    11041 agtccgcagg atcgcgaaaa gcgtcgatgc gcaaggcagc gaagaaatga ccggtattgg
    11101 cagcgccaga aacagccggc cccaaaacgc gctcgccaaa accagctcct gccagcaccc
    11161 ctgcaaagat atccaccagc agcgccaacc cataaccttt atatccgcgc aataattccg
    11221 ggccgcccag cggcatcagc gcgccgcctt cgaccacacg ggaggggtct tctgtaacat
    11281 ctccttcgga gttaatcccc caacccgcag ggattgacct gccttctttt tgatagacgg
    11341 tgatacgccc aatgggcacg atgctggttg ccatatccaa cacgtaaggg cgggcgccgc
    11401 cagccggcac agccaccgcg attggattgg tgcccaacat tgcctttcgc ccatgcgtgg
    11461 gggctaccag cggagcggag ttggtaaggc tgatgccgat catgccttct gaaagcggca
    11521 tcatggcata ataacctgcg ataccgtaat ggttgctgtt gcgcacagtt gccatggcca
    11581 accctgcatt gcgcgctttt tcgatgcaca tcctcatcgc cttcacacca gccggatgcc
    11641 caagcccgtt gtgcgcatcc aacgacaatg tggttggcgt ttctttcaga gttgtcagcg
    11701 ctgctcgcgg gttggtcagc ccactttcca ggcgggaacc ataatagttt tgcaggcgaa
    11761 taaggccatg agaatccacg ccgcgcgcgt ccgcgctgac cagcacatcc gcggtgattt
    11821 cggcgtcttc ctctgaaaca ccccagcggg tgaaatatcg gctagcgaat tctttcaaat
    11881 cctcaatctg ataataacgc atctggtttt ctccaatgtt gaagtggaga ggtcattata
    11941 cttgattgtc cggcgagcca atgcataccc gccttggtga atcacaaaat ccttttctct
    12001 tgtcgctgtt cgctcaattg aaaactggct ccagcgcaaa ctggagccag aagtgaacgt
    12061 ttagccttcg tcagaatgca ggcgtaatca atgagtaagt ggcatcaggg cgagattctc
    12121 gcctaactca acccgatgat tctgcccgtt tcaggctcaa tatcaatctc catgaatatc
    12181 gggcgggtcg gcagccctgg catggtggac atagtgccca gcagcgggta aaggaacccg
    12241 gcgcctaccg aggcgcggat atcgcggatg gggatacgaa cgcctttggg cacgccttta
    12301 agagtggcgt catgtgaaag gctgaggtgg gtcttggcca tgcagatggg cagtttgtca
    12361 aaccccagtc gggtgtattc agcgatacgt tcctcggctt cgggcaggta atctactcct
    12421 tccgcgccat agatctcgcg ggtaatgcat tcgattttct gcttgatgga ccaatccagc
    12481 gggtagagaa actcaaattt ggctggtttc tcggccgccc ggatgaccgc cctggccagg
    12541 tcgaccgcgc cttcccctcc cttttcccaa tgctcgcaga ccaccgcgtc ttccgcaccg
    12601 ccctcctcca ctgcggattt gcgcaccatt tcaagttcag ccgggctgtc attgacaaaa
    12661 cgattgaccg ccaccaccac gggaatgccg tatttgcgcg cgatttttat atggtgcaac
    12721 atgtttggca gaccagcctt caacaattcc aggttttcag aggtgtaagc ctcatccaat
    12781 ggctttcctg ccaccacctt gggtccacct ccgtgcatct tgagcgcgcg aatcgtggct
    12841 accagcacca ccacggaagg gatcaggccg gaatagcgac atttaatatc cataaacttt
    12901 tccatgccca tgtccgcgcc aaaaccgctt tcggtcacca cgtaatcagc cagtttcaag
    12961 gcgatcatat ccgcgagtat agaagaattt ccatgcgcga tattggcaaa gggtccggcg
    13021 tgcacaaaga ccggggtgcc ttccacggtt tgcatcaggg taggcttaat cgcatcgcgc
    13081 atgagcaccg tcatcgcccc ggccacgccc aggtcttcgg ccgtgaccgc gtctccgcca
    13141 tggctgatgc caatgacaat ccgtccaaag cgttcgcgca tgtctttcag gcttgtggtg
    13201 agggctagga tggccatgat ctctgatgcc accgtgatat cgtaacctgt gcggcgggtg
    13261 aaaccttttt catccggtcc ttcgccaatg gtaatgccgc gcaggaaacg atcagaagta
    13321 tccaccacgc ggcgccaggt gatggtatca gggtcaatat ccaggcggca gagttgccgc
    13381 ctttgctcct cgttcagttc atccgggtct gaaacgctga tgcccagctt ttcaagcctg
    13441 gcgaccattc ccctggtaaa gtggcgtttt ccgttcttat cttttggcag caggcggttg
    13501 aacagctgct cgtctgtagc ttccttctcg tggaacatgc gcgcgtcgat cgctgccgcc
    13561 aacaggttgt tggcagcggt gatggcatgg atatcgcccg tgagatgcag gttgaattct
    13621 tccatcggta caacctgtga gtaaccaccg ccggctgcac cgcctttgat cccgaacgtc
    13681 ggcccctgtg aaggctggcg gatgcaggtg atcacgctct tgccaaggtg cgcccccaac
    13741 gcctgactga gccccaccat ggtggtggat ttgccttccc ccaacggcgt cggcgtgatg
    13801 gccgttacat caatgtactt cccgtccggt atgtgcttta gccgttgcag cacttcaagg
    13861 cgcaccttag ccttggtatc gccgtaaggt tctgtttcag caggcagaag acccaactcc
    13921 tccgcaatta acatgattgg ttttaaagtc gcctcttggg cgatttcgat atcggatggt
    13981 acgggcgtgc gtcgtttcag cggggttgga gtgaaggctt tgcgaccgtg gaatttctca
    14041 gaagaggttg tcattttttc tccttgacaa ttgatctaat caggttaatt aaagacgaaa
    14101 tagagaggaa atgcaagcga aaaaatcctg gtaaaccacc ttgttcttca tgaaaactgg
    14161 ttgaactatt cagataagta cctgctataa taatttcaga ttagatatgt tctaattcaa
    14221 tcgtcgccaa aaccaaagga ggtaaaaatg gcagtaatcg gatattgcat gaaatgtaaa
    14281 aagaaaaccg aaatgaaaga caccgtggaa gtgaccatga agaacgagcg caaagcgatg
    14341 aaaggaaagt gctcagtctg cggcaccggt atgtacaaaa tcctcggtaa ataagccgga
    14401 ttctttctac catgaaaaac cgcgcctaaa ctctggtgcg gttttcgtta cggcagattc
    14461 cagctgctcg ccatccatga ttgccctcaa atcgtaacgt taaactactc ataagccaaa
    14521 gggatgatcc aatcatacac cgcgggcgaa tagcgctcca gatgagaaaa aaaatctttc
    14581 agttcgcaca aaaacaggct gacccaggag aggatacttc tgatgttgcc cattgaagag
    14641 cctgtcgtct ttttcctggt catcatggcg atcatcctgg tcatgccgct gctttccgag
    14701 cgggttcact tacccgggat cgtaggggtg atcatcggcg gcatgctgat tgggccgcac
    14761 gggttcaaca tactggcggc aaacgaccgt atggaatttc ttgccaccat cgggctgatc
    14821 taccttatgt tcagcgctgg tttggaagtg gattttcacc agttcatgcg cataaaaggg
    14881 aaagcggcca tcttcggttt gatgaccttt gtcatccccc aggtgtttgg catggcgttg
    14941 gggtggatga tccacatgcc cttgctcggt tgtgtccttc ttggctcagc ttttgcttct
    15001 cacacgctgc tggcttttcc cattcttacc aggctggggg tgaccaaaaa cgaagccgtg
    15061 gcggtaaccg ccggggctac cattctcacc gatatcggcg ccttcatcat tctggcaatt
    15121 gtcttgggtt caaacggcgg gcagctggag cttggttatt tcatcaaaat cttcgtcttg
    15181 cttgtggctt tcgccctcgc cactctcttt atccttccca ggctggggaa atggttcttc
    15241 cagaaggtca aagggcgcac ggttgaattt caattcgtca tcgtgttgct tttcatcgct
    15301 gccatgggag ccaagctgat tggggtgcat gaggtagtag gagcctttct agccgggctg
    15361 gcggtcaacg ccacgcttcc gcggcacagc cctgtcacca gccatgtcct attcatgggc
    15421 gaatcctttt ttattcccgt ttttttactc ttcagcggca tggtcaccga tccgttaacc
    15481 ttactgcata cacctggagc gctttttctg acgctcggca tcctggtcgt ggcttatctt
    15541 tccaaattcc tggcttcgct ggtcacctcc aggttattca aatacagtaa agaagaattc
    15601 ctcaccgtgt ttgggttgtc tcacgcgcag gctgctgtta ccatcccgac attggtgatt
    15661 ggcatgcagc tgaacctgtt ttcaacggaa ctttttaatg cagcaatgat gatgattctg
    15721 ctcacctcca tcacctcccc tctgattgtg caaaaatttg ctccagggct gcaccaggaa
    15781 gttaccgaac tggaagagct gcccttgttc caacgcgtgc tggtacccat cgccaaccca
    15841 ggcacgcagg aacatctgtt ggctttagca gggctgttag ccaagagcgg cggcggtgag
    15901 gtgatcgcgc tcaatgtggt gctggaccgt aacgaccaag gcgccaatct ctcgcagcaa
    15961 aaagagcttc tgaaccgggt gccggagctt ttaaaagacc ctgaaaccag gtacgaatta
    16021 gttccgcgcc tggctgattc ttacgccagg ggcatcctgc aggtctcgcg cgagcgcggt
    16081 tccaccttga tcgtgatggg ctggcgtggt aaacgcactt ttagaaccag cgtattaggc
    16141 acggtgttgg acgaggtgat ttggggctcc gacactcctg tgatggtagg aaaactgagc
    16201 cgctcactta atggcacgcg cagggtcctg ttactcatcc cggcgggcgt ggtgtccccg
    16261 tctgccctgc gccgggtggt gcaggccaac atgacgctgg cgcgagaact gaacgtgccg
    16321 ctggaagtgc tggccgcgaa gagttacctg gagttgatta aacgcattat cgggaaaaat
    16381 cctaaccaaa ttgccatcac ggttcaagaa atggtcgggg ctttacgctt aaaagacctt
    16441 gagaaaaacg tggagagtga cctgctggtc attccagggt ttgggtcgcg caagcacttc
    16501 atcgccagcc tgggcaatct gccagaacgt ctggcagatt cattctccgg caacctgatt
    16561 attttgcact ttgaccgctg aacaggtttg ccagcatttt ttgggctgag cggaaaatag
    16621 attatgggtg ttcaccttcg agcctggccc ttaatggcag caacgtatcg gtatcaaaat
    16681 catagcgttg cagatgttgc ataatcagtt ctagcgcctg tgattcattg atctgcgctg
    16741 aacagggtgt tgcgtatgct gttatatttc cgtgggtcat actcatttcc accaggtcca
    16801 ggtcatacca ttgatcacag acaatgactg aaatcctccc aaactgaaaa aaaggcggtt
    16861 ctgactcagg ataaatcacc ttgccagtga cagggtcttt ggatgatttc gggatcctga
    16921 cagtaaagat tgggcgctca atataagaag gagatagcaa agattgcccc gccatccatt
    16981 tgggcgtttt gatgccaagg taatccagca gcgtgggggc gatatccaca gtgctgacgt
    17041 tctggtcaag aacgccctgg cgttcagcgc gagggaagcg tatcaataac ggcagcctgg
    17101 aattggtcca tttctggctg tgatcgctgg tgaccaccag cagggtattc tccagctgat
    17161 tgcttttctc aaggcgcgcg taaatctctt caaacgcctg gtcaaactcc agaatcgcgt
    17221 catagtagaa caactggttg ttgtttccct gttcatcaac acttatgccc gccgaaaaca
    17281 cctgcttatc aggaaaatag cgcggtccat gcgtgcccat ccagtgcagg tgcgcaaaaa
    17341 ccggctggcg gctttcttca agaaggcgga atagataatc gattttttcc cagtcattga
    17401 acttcaccgc ggcttcggtg acctgttcgt aggggttaga catatccgaa aggaaaaaga
    17461 tatgacccag cctgtcactg agcctgtcgg tcatctcctg ttgaaataga tagttgattc
    17521 ccgggtagat cctggcaagg gcaaacgcca gcggttgttg atcagcagaa cggccgttgg
    17581 cttcattaaa agcgttctgg aagttggttt tataggcatc agcgtaaacc gggttgctga
    17641 gctgaaccgt gtaatagccg aagtcctcga ggatttccgg caggtgctga taggcatctt
    17701 ctccctgtaa caggtcagaa gaatagatca ctcgcgtatc catggggtat ttgccggtca
    17761 gcagggtggt aattgaacca atggttccct gcgcgttggc aaatgcgttc cgtgccctta
    17821 acgattcggc agaaatcgcc cgtagaaaag gagtggtgtc tctttcatag gcatcaagag
    17881 aagtgtattc agcgttcacg ccgtccaccg tgatcaacag gatatcaggg gggatggagc
    17941 ggctccccgc agggtggaag cggctggaac ccaatagttg attgcccatg gacagcgcgg
    18001 ggatgccggc gctggccagg ctgaggagga agatactacc cacaagcatt agccgttgag
    18061 caggcttcca gcgtgaaaag aacgcagcca gaaggtggtg aaaggccacc agcttgaaga
    18121 acaggtaaac gaccagaaag gaaaacagca ccccatacag cccgcggatc cagtccctgg
    18181 tcgaaacaat gcctaattgg aaaatagtgt aagtgaagtt atccagcagg agtaaagcca
    18241 gggcagccag caggaatgca ggcagcagca tggcggttaa ggtgagcatt tttctgaatg
    18301 cagcctgctg gaaaacctgc ccgctcgcca ggaaaggcaa cagagccagg ccgcaggcca
    18361 gcaccagtaa accagaagcc gtggatagcg ccaggaattt ttggaggaaa gcaatagtct
    18421 gtagaaaaga agggcgcgtg aaaaggaaaa tccattccat caagatataa agcgcggcga
    18481 tggcgctggt gagcaccatc aggctggtcg gtgaccaggc ggggcgcatg accgggcgca
    18541 gcagttcttt gatggatgag tttttcttga gggttggcat caacacctat ccgtttttga
    18601 cggggctaat tttaaaccat taaggtacac gcaggggaaa accaaacgag gaaggtccag
    18661 aggcgccaaa gtaaattgtg cggaaggttc gcgaagacca acgcaaatca gcgggcgcgc
    18721 ttgagccatc aaaaaaatag tttttcttca tagctcgtat tcaccagccg ccaaacctcc
    18781 attggaggaa ggttcttcgg agagcaggaa gcgctgccct ttggattgct gttacagttg
    18841 gaagtggggc gagagggggt cgaaccctca cgatgtcacc atcgacggat tttaagtccg
    18901 ttgcgtctgc cgattccgcc atcgccccaa acagtttcta ttttatcgca attcacaaaa
    18961 atcggagtga aaaatcgcct gccaggtgcc ggttcccgtt gccatgcgcc agttatctcc
    19021 atctcttgac cgctttcgcc tgtaccgagt aaaggtgaac cagcggtgaa gagggttccc
    19081 cgcacacccg cgctgaacgc atagccaggc gcaggctttc agcatcgttg aattcgggaa
    19141 cgagcgtgta catgcgcccc acctcgctgg gatggtgaga gtctgaaccc gcggtaccgg
    19201 gcagtccaag gctcgccgcg tattcggcag cccgttggtt gtacgcgggg gtgagcacac
    19261 ggccgttacc ggtctcgata gcgtctacct gcgaggcgat gaggtccagg gtttcacggc
    19321 tccagcctga gcgctgcggg tcaaagggat gggagatgct gatgaacgcg ccctgtttgc
    19381 gtaagcggtg cagcgcctcc agcggctcga gcccgcgcgg aacaacgacc gttacgaaag
    19441 cagccagcaa ctcgccctcg ctggtttgca cttcttcgcc gggaatcacc aggtcaggcg
    19501 ccgcgcgcca ggcttccagc gcgcctgtca gtacattgtg gtcagttacc accacccggt
    19561 ccaacccgcg ttttcgggcg gcggaaatta gttcatgaat gggggtaagg ctgtcttcag
    19621 aatagcgtgt gtgtgtgtga aaatcaactt tcagcaaatc cgctcactcc tggttccgcc
    19681 gtgcagggtt tcgctgcggc agaccagaag agtataccaa agagcaggct aatccagtag
    19741 gtagacatac ggaaaaggat ggtggcagta acggcaagtt cgggttgaaa ccccagtaaa
    19801 acggtgagca agccagccag cgccagttca accacaccta acccaccagg aacagtggag
    19861 aagaaaccaa tgatgatgga aaacgccagt accaggcagg cgatggccgc aagggtgagg
    19921 ctgggttcaa gcccaaaacc aaccaagacg aaaaagagcg cggcggccag ggcaatccac
    19981 gccagcacgt tgaaaaaaag cgataccgtc atcggaaagg ttctgaaaag atgttcattg
    20041 ccttccatac actggcgtaa ctgccggcta aacaggcgca gcagaggaag cttttccccc
    20101 gcatccagca aaccttctcc agccggcttg atctgtaaga agaggatgga taacacaaac
    20161 aacaacagca tcgccgcgaa gaatggccag aaagcggggt aagccaggat gcccagcgtg
    20221 gccagcgcca aaatactcaa cccgtcagaa aggcggtcga taaggaaaat tgaatcgatc
    20281 cggacaagcg ggatgccgca ggctttactc aaccaaaccg agcgatagga ttcctgggct
    20341 tttgggtctg cggcggtcag cgctgagctt gaaaggtaga aagccatgcg tttttttagg
    20401 ggaatcctgc gaattccaga tatattcaat gagaaagaaa ggttgaggaa gcgtagggca
    20461 tgatggatga tgttcaaccc taccgcgacc gcaaaataca cccacttgaa agactcaaac
    20521 aaggtgaaaa cctggtggat gctgctgaga gatatcagca gtgcaaaaac ggccaatccc
    20581 aacagcaacc cgggtaacag tacacgatag ttctgacggg tatctgtaat catgttttcc
    20641 tgctcctcag atgattataa cgaagattag cggcactcca gtttacatgc ctgttaagtt
    20701 gtccatcctt aaatcacatg cgctcagtgt tgggtttaag cccatcattt tcaagccaag
    20761 caagttgtga agcgccattc tcatagcgat agggtataat tttaattatg aatgaggaaa
    20821 tgtgtgctca tactgaaaaa cgaatgaacc ctgatgatcc cgatgacctc agtatgatta
    20881 tttggggttt caacctggtt ttgtagcgtc aaaactgcac ctccaggtcg atgatatcct
    20941 gaaggtgatg aagagatgac gcaaaacacc cgtgtttttg gcacatcgca aaatagccgc
    21001 aagcccctca cctggggcga tattatcgcc ctttccctgg tgatcgcatt attgtatgtg
    21061 ggggcgcgct tagcggtcaa cgcgcctgaa gtcgtccctg gaccggatat ttcattatct
    21121 ccatcagccc tgccaatata tacgcttttt tcagttggtc gaatggcagc cgcctatctc
    21181 ctctctttct tgttctccat catttacggg cgagtagccg cttatcatca aaatgctgaa
    21241 aaaatcctga tgcccttatt ggatgtgctg caaagcgtgc ccattttatc gtttctgcca
    21301 gtcgtcttat tgagtttaag ttccttcctg ccagaaggaa cagcggctga gattgcttcc
    21361 atcgtattga tcttcaccag tcaggcttgg aacattacct ttgcctggta tcaatcgctg
    21421 accacaattc ccaaagaatt aaaagaagcc agttctatct ttcgcctgaa taaatggatc
    21481 aatttcaagg cgctcgaatt accgtttgga atgatcagcc tgatatggaa cagcatgatg
    21541 tcttgggcag gcggttggtt cttcttaatg gcggccgaaa tcttcaccgt gggctcacgc
    21601 gattttcgtc tgcctggatt gggtgcttat ttacaggaag cggctaatca aagcaaccat
    21661 gtggccattt ttctggggac aggcgtgttg atctttaccg tcgttctcct ggaccaactc
    21721 atttggcgtc cactgatcgc ctggtcagat cgatttaaag ttgaaatggt cgagaacgac
    21781 aacccgccaa cctcgtggtt cctggatctg atgcacaacg cgaacattac ccgaattatc
    21841 tcaaaccgaa ttttcgcccc gctgacggag tggtttgacc gcttaacaat caaacatgcc
    21901 ccacctgtct ccaggttgga aaatggtaaa aaccaactta aatggtatgt gattgttttt
    21961 tccatcattg tgtttgcctt gatcagctac gggctttatc acgcaacaga aatgttattg
    22021 tccgtccctg ttcaaaaatg gggggtcatt gggttgggag ttacagccac ttttctgcgg
    22081 gtaattgtgg cattattgat tgcgcttgcc tggaccattc ccgtcggtgt cctcattgga
    22141 acaaatcgcc ctttagccac cgtgcttcag cctatcgtcc aggttaccgc ctctgttcct
    22201 gcaacagccc tttttcctgt tctgttactg cttttcctga atgcccaggg aggattgaac
    22261 actgccgcgg ttttgttaat gctcatgggt acccaatggt atttgctatt caatgtcatt
    22321 gccggagcga gcgccatccc gcaagattta atttttacct caaaactcat gggtctcagt
    22381 ggctggcagc gttggcgaac gttgttgctc cctgctattt ttccttacct ggttaccggg
    22441 gcgattactg ccagcggcgg agcctggaac gccagcattg tggctgaata ccaaaagttt
    22501 ggcgaaaaaa cacatactgt aaccggtatt ggttcgctga tagcgcaggc gaccgcctca
    22561 ggtgattacc ctttactgct ggcgggtaca ctggcgatga ttatcactgt cgttgtggtc
    22621 aaccgagtga tctggcggag gttgtaccgt ttagccgaag aaaaataccg aatggagtga
    22681 aaatgacaga agccttgttg caactcgagc atgttttcca gtcttacggg gctggcgtgc
    22741 gtcgttttac tgcggttgag aatgtgaacc tgaacctttc ggtgggcgaa tttgtcgccc
    22801 tgcttggtcc atctggttgt ggaaaaagca cgttgctgcg cattattgca ggcctacaaa
    22861 aagccaccca gggaaaggtg ctttatcgtg gtcagcaatt agaaggagtt aacccccata
    22921 catcgattat ctttcaaacc tttgcactgt tcccctggtt aactgtgctg gagaatgtgt
    22981 cgctcgcgtt aaaggcaaaa ggtgttgaaa aacacgagag agacaaaaaa gccgaagact
    23041 tgctggaccg tgtaggcttg gatggctttg agtctgccta tcccagagag ctttcgggag
    23101 ggatgcgcca aaaagttgga tttgcccgtg ccatggcggt tgaacctgag ttgttatgta
    23161 tggacgaacc tttttcggct ttggatgttt taagtgctga atccctgcgc ggggaactgc
    23221 tggaattatg gaccgaaggc aaaatcccaa ccaaggcgat tttgatggtc agccacaata
    23281 tcgaagaagc cgttttcctg gcagaccgta ttgtggtgat ggataaagaa cctgggcgta
    23341 ttatcgccga gcataaaata gacctccccc atccgcgcaa tcgtaaatca gtcgagtttc
    23401 aaaatcacgt ggaccaggtt tacgcggttt tagccggtca aacacgccca gaaaaagaag
    23461 agctgggcac agcgcctggt gaagtgggta tcacccgtca tcttcctgag gttgaaataa
    23521 gcgatttagc cgggttattg gaaaatctcg ccgaaggagg taatcagaag cacgatatct
    23581 acctgttaga agataagctg ggcattacat ccgatcaatt gctgaagcta acagaggctg
    23641 ccgagttatt gggtttctct tcgatcgcca agggagatat tgaattgacc gctttgggtc
    23701 aggcttttgc agacgcgagt attctagccc gtaaagaaat ttttgcctct tgcgtcaggc
    23761 gaattcctct catgcgctgg cttctcagta tgcttcattc tgcagaagat agacgcttaa
    23821 aaattgaagt catcctcacc gccctgagcc ttgatttttc aaccgaagaa gcggaaaaac
    23881 aaatcgaagt cctgatcaag tggggaaggt atggagaatt attcgtttac gatgacgacc
    23941 aagaaatgat cgaacttgaa gaggggagat aaaaaaaaac gcaaccaagc cttcaccatc
    24001 ctccgcgaaa gtgagaaagg aacgcgagag cagcgctttg aaggttggtt ctcagttagc
    24061 cggtacggct gggttcagta gcgtcgttac gaaaatgtgg agagcccacg cggctgccgg
    24121 aacaccagca tccccaatcc aaaaaggatt cgccgctttt aggatgaggt tcctcaaaaa
    24181 aagtcaacct gaccttgacc agtatttgaa gagtgtgata aaatagaaac ggttcgaaag
    24241 acatttgagg tttcaacacc gaaaagtggg cattccccac ttttcttatt atcaaaactt
    24301 catatcgacc attttgaaaa caggtggaga ataacaaatg aagaacgagt ttactctggc
    24361 gttcaacgaa gttctcgaag ataaagggct gcccaaggaa gtgatactgg aagcgcttga
    24421 atctgccatg gtttccgctt accggaaatc agtgaatgct tcaaacgaac aacccatcga
    24481 agcgaaaatc gaccttgaga ccggcaaagt gaccatttat gcccaaaagg aagtcgttga
    24541 agtcgttgaa aataacatca ccgaagtaga gcttgagact gctcgcaagg tggagccaga
    24601 agcccagttg ggtgacctgg tagtcgtaga atccaccccg ggcgactttg gccgcgtggc
    24661 tgctacgacc gcccgtcagg tcatccagca acgcattcga gacgctgagc gcaaagccca
    24721 gataagttac tacgaaaaac agcttggcga gatcgtcagc ggcgtggtgc aggcgattgg
    24781 cgcagcggga ttgacgatcg gtctcgaatt gaaaaccgag gccaccatgc cccgtaaaga
    24841 gatgattacc aacgaacact tccatatcca tgagcgtgta cgcgcgcttg tcgctgatgt
    24901 gaaagattcg ccgcgcggtc cgcaaattat cctctcacgc acccaccggg atttcttgcg
    24961 ccgtttgctt gaaaatgaag tccctgagat attccacggg atcgttgaaa tacgttccat
    25021 cgcccgcgaa cccggtgacc gtgccaaggt ggcagtatcc gccacacagc agggcataga
    25081 cccggttggc gcctgtgtcg gaatccgcgg cgtgcgtatc caggcaattg tcaaggaact
    25141 gcacgatgaa aaaattgatg tcatcgagtg gaatcctgat ccggctgtat tcatcgccaa
    25201 agccatcagc ccggcgcggg tcagcggcgt gtatctcacc gaccagcatt ctggtaacgc
    25261 taaaacagcc acggtggtcg tgcctgaaga ccagttaagc ctggctatcg ggcgcattgg
    25321 gcaaaacgcc cgcctggcgg ccaagttaac cggttggcgt attgatataa aaagccttcc
    25381 cgaggctgct gctgatgccc tgcaaaaact ggcgactgac cccgcagttg aggctttaaa
    25441 agaaagtgaa catgacaacg cgctccgcat cgaagaactc ctggcgcgta aagaggaagg
    25501 caaaccgctt tctcctgaag agtacgacca gatggcgaaa tttgtagacc gtgtcgaacg
    25561 gaaatccatt gccaagagcg aggagcagcg taaacaggaa cttgagaaac tggcagaaat
    25621 caagaaacag atccccgcgt cagccttcga actgaacatt ctggacagcg gattacctga
    25681 acatgttgct gtcatcctcc aggaggccgg ttacttcagt gttggtgacc tggcaatgca
    25741 ggtgaagcag gacccagact tgattcttgg tatcaacggc atcggtatcc gcgcgatgga
    25801 atccatccat gagctcatgg cagcgctgga taagcaactg gctgatgaac aagctgcgct
    25861 cgtcaaacaa gccgaagaaa ccgctgagga gatggaggtt ttggaacccg tcagtgaaat
    25921 tgagctgccg gccgaagttg atgttgtccc ctctgtcgag gtcgaaccgg tgctcgatgt
    25981 cactgtgggt gaaacctcat caactgagac cattgaagaa ccggagcccg agaaggaacc
    26041 ttctcttgag gaactgttca cgctgcgccc agaagtgcta gataccgccg gcttgctgga
    26101 tgacgatgaa gcggaagaga gcggtaaagc caagaaaaaa gggaaaaaga agggcaagag
    26161 cgttgtggta acgtatgacc ccgatcgaga tctgaccgtg gtacaaaaga aacacaaacg
    26221 tggtggtaat gattgggatt gggatgagta aaaacgtgag aaagacaggt ggctaagaaa
    26281 cctcaaaaac ggatcaaaca cctcccacaa cgcacctgta ttggctgtaa aacggttctc
    26341 gccaaacagg aactggtgcg gttggtgcgc acgccagagg gtattatggt ggacgaaacc
    26401 gggaaagccg caggcagagg ggcctatgtg cacaataccc ggcgttgctg ggagcaggcg
    26461 ttaaaaggtc ccatcagcca cgcgctgcgg acagacatca cccccgcaga ccggcaacgg
    26521 ctgaaagagt accttgaacg gctgccagag gttgacccaa tagaaaatcg tgctgagagc
    26581 agagatgtaa ccaactgatt caagaaaacc gctccaggca catccaccgc cctttcagga
    26641 attggtgaac ataaacagat tacatgacag attgtagggg gaacaacttt ggaggtgtgt
    26701 tatgagcgaa aaaacgatcg aatttccctc acaaatcact gtaagggacc tggcgcaaag
    26761 aatggaagcc agcccaattc aggttatcaa gatcctcatg tcaaacggcg tgatggccaa
    26821 catcaatcag caggtagatt ttgataccgc ggcggttgtg gcttctgaac tgggttttga
    26881 ggctgttctc gaatcagtta aagaaaccgc gccggctgaa gaccctggga aggtgcccct
    26941 gtggcgcgag ctgatcgata gcgaagacaa atcgaaattg atcaaccgcc ctccggtcgt
    27001 caccattctt gggcatgtgg accacggtaa aaccaccctc ctggatgcca tccgccacgc
    27061 caatgtggcc agcggagaag ccggtggtat tacccagcat attggcgcat accaggtaga
    27121 acacaagggg aaagtgatca catttctgga tacgcccggg cacgcggctt tctccgccat
    27181 gcgcgcacgc ggcgcccaag gcgctgatat cgtcatcctg gttgtggctg cagatgacgg
    27241 ggtgatgcct caaacgcgcg aggcgatcgc ccacgccaag gcggccaggg tgcccatcat
    27301 cgtggcgctc aataagatag accgcgcgga cgccaacccc gattttgtga aacgtcaact
    27361 ctccgagcag ggattggtgc ccgatgaatg ggacggcaac accattgtgg tgccagtgtc
    27421 agcaaaacag aagaaaggat tagatgatat cctggaggcg gttcttctgg tgagtgagag
    27481 cctggatatt aaagccaacc cgcacgggaa agttttcggc accgtgatcg aagcgcaggt
    27541 ggataaagca cgtggggtaa tcgccaccct gctggttcag aatggaacgt tacagaccgg
    27601 taacacggtg attgtaggtg acacctgtgg gaagattcgc gccatgtatg atttcaatgg
    27661 gaaaacggta aaaagcgcct ttccttcaac acccatccag gtattgggga tcggcaatgt
    27721 cccacccgcc ggtgaattgt tccaggtggt tccaagtgaa aaggaagccc gcctgatatt
    27781 ggcagagcgc gccgccagca aagaaaacgg atctgaaacc tccactcgca cctcacttga
    27841 gaaattattt gaaaaattcc aggctggcga ggttcgtgaa cttcctttga ttctcaaggt
    27901 ggatgttcag ggttcggtgg agccgattgt caactctctg aacgaattaa gccagggtga
    27961 gatcaaaatt aacgtgatcc atgctgaaac ggggaacatc agcgaaagcg atgtgatgtt
    28021 ggcagccgct tcccgcgcca tcttactggg attcaatgtc agcgccgacc aggctgcccg
    28081 caacctggcc gaacaggaag gcatttccat ccgtttgtac aacatcatct accgtatgat
    28141 cgaagacatc gagaaagcgc ttaaaggtat gctggaaccc gagacccgcg aaattcccgt
    28201 aggaaccgcc caggtgcgtg ccgtgttcaa gatcagcaaa gtgggcacca tcgcgggctg
    28261 ccgcgtgcta acgggagagc tgcggcgtaa tgcccgtatc cgcctgttgc gcgaaggcga
    28321 aaccctgttt gacggcgaga tctcatccct caagcacgaa aaagacgatg tgaaagaggt
    28381 ccgccagggg tttgagtgcg gtgtatcgtt gaaagggtat aatgatatca acgaaggcga
    28441 cctgctggaa aattacatca ttgaaaaatc cgcttagtct gagctaaagt acaggtactg
    28501 ctatgccttc accattacgt ttaacccgca tcggcgatcg attccgcaag gaattatccg
    28561 agatgcttgt caaagaagag atccgtgatc cacgcctggc tggaatatcc atcacggatg
    28621 tccgtgtaga ccgtgaactg tcttacgcgg atatttacgt ttcagcggtg gaaggggctt
    28681 cgcgatcgaa agaagtgctg gaaggattgg aaagcgccag cgggttcatc cgcaaagcgc
    28741 tgtcagaccg aatccaatta cgctccttcc cgcgcctgcg ttttcattgg gatatcaccc
    28801 ccgagcgcgc agaccgtatc gagcagttat tggcttccat acgtgaagaa gaaagacaac
    28861 agaagaagaa atgatcgatc ggcaaatcaa agaactgctc caccgctcaa aaaagatagg
    28921 gctcacctct catattcgcc cggatggtga cgcaatcggt gctgtgctcg gactcggtct
    28981 ggctttaacc gccgcgggaa agcgcgtgga aatggtgctg cgcaatggca tttcaaaaac
    29041 cttcagacac ctgcctgggg cagagagaat tcaatcagcc ttcaaaacag agaacgacct
    29101 gtacatcgtc ttggattgca gtgacctcga acgcaccggg ggtgtattga acgggcgcga
    29161 agttgatatc gtgatcgatc accacatcac aaataaaggt ttcggaaagg tgaatttgat
    29221 cgaacctgaa tcggtggcaa cctgcgccac cctggcgcag catatgcccg actggggatt
    29281 ggttattgac aaagcagtgg cgtcggctct cctttcgggt atcattagcg attcgatcgg
    29341 cttccgcacc tccaacacaa ccgccttatc tttacgcatc gcggctgacc tgatcgaaaa
    29401 aggcgctgac attaaccagt tgtataatag ggcacttatc agccgtcctt tcaccagcgt
    29461 tcactattgg ggatacgggc tggcaaaact tgaaagagaa aacgggctcg tgtggacaac
    29521 gctatcactg gcagaccgcc acaacgccgg atatcaagaa gacgatgacg cagaccttac
    29581 caacatactt tccagcattg aggatatgga cgtggtggtg ctttttatcg aacaaaaacc
    29641 agaccaggta aaagtcagtt ggcgcgcccg cccgggtatc aacgtggcgt ctattgccga
    29701 ttctcttggg ggaggcggtc acccagccgc agccggtgtt gaaatggaag gcaatttagc
    29761 acaggtaaag gatttgattt taagtaaaac cagagactat cttcgctctc tgaaaccaga
    29821 aaacggttca ccccgggata gcgaagtgac ggagcacata aatggaaaat aactcaggga
    29881 tcgatgttaa gaatgccatc tcgggtgtat tggttgtgga taagcccgtg ggcatgacct
    29941 cgcatgacgt ggtacaaatc attcgtaaag gctccaccat caaacgcgcc ggtcataccg
    30001 gtacgcttga cccgcgcgcg tctggcgtgc tggtggtgtt gctcggccct gcggtgcgct
    30061 taagcgaata cgtatctgcg tcggataagc gctaccaggc ggtgatcatg ctcggtacca
    30121 ccactgatac ctatgacgcc gacggacgga ccacctcatc tgcgccggtg aacatatctg
    30181 aagaagaatt caataacgaa ttgcaaaaat ttgttggtca gatcgaacag gtgcccccgc
    30241 cttactcagc cgttaaagtg cacggtcaga aagcttacga tatagcgcgc agaggagagg
    30301 aagttgaact ggcaccacgc atcatacatg tgtacagcct ggagctgctt gagtgggcgc
    30361 cgcctgaggt ggtcattgac gtgtactgct cctccggcac ttatgtgcgt tcgctggtaa
    30421 acgacctcgg ttcgcgtttg ggctgcgggg ctaccctttc aggtttacgg cgcacaaaaa
    30481 gcggccgctt cacactgaga gacgcggttc ccctgcgtaa actgaatgaa gcctttgccg
    30541 atggatcgtg gtatcaatac ctgattccag ccgccgaggc tttatccgac tggccgatga
    30601 tcgaacttaa cgaccaggag gtcgattcta tcaagcacgg tatccgcatt cctgctgttg
    30661 atgcgaaaca acaaaaagtt tgcggcgtga gcggtcaggg agaactggtg gcgctgctgg
    30721 aactcgacgc agaaaaaatg gaatggcagc ccaagaaagt gttcttctcg taaccacttc
    30781 cctctcgcct ggtcgagcgg gttctctcca tatccaaagc agaaacgcat gcggatataa
    30841 ttaacgcatg aactacatcg aagcgttaca acaagcaaac ctcaaggaat cctgggcgac
    30901 aatcggcgcc tttgatggag tgcatatcgg gcaccgcacc ctctttctaa aactcgttga
    30961 tggtgctcgg caggatggct gcaaggcagt tgccattacc tttgagccat tgccagcgct
    31021 tttctttaac cgcatcaaga acggtcaggt cctgacaaca ctcgctcaac gggtttcatt
    31081 gatcaaagcg ctggggattg atgaggtgat tgttttagat ttcgaccagg cgcttgcaga
    31141 tgtggaagcg ttcccttttt tgcagcaggt tcacaaagcg attgggttaa ggaggttgct
    31201 ctcgggtttc aattcccgga ttggcaaaga ccaggctggt gctgtaccca aactgcaaga
    31261 gattgggcag gtactgggct tctgcgttga agttgtaccg cccgtcaaga atggccagga
    31321 aatcatttct tccagcaata ttcgtaaact cctcaaagct ggagaaataa cacgagccaa
    31381 tcagttcctt gggcggcctt atgctcttat aggtgaagtg gtacacgggg aacaccgtgg
    31441 cagtaagtta ggtattccca ccgccaatct ccgccttcca gcggagcaaa tgctgccagc
    31501 cagtggcgtg tacgcttgca gggcaagtgt gggagaactg gccttcattg ctgtaaccaa
    31561 tgtaggcatc cgccccacat ttgaaaaccc attaccagtt ccgcgtgtag agccgcatct
    31621 gctcgatacc agcgaaacgt tttacggcgt caacctggaa ctcgaatttt tcgcattcct
    31681 gcgcccagaa atcaagttcc cagatgccaa agcgctggtt gcgcagatac agctcgacat
    31741 tcaaaagacc agggagtatt tcgcaaatgc agcctaaacg acagatttat ttactcgatc
    31801 cacaacaatt gtcccctgag accatcgctg tgacctttgc caaaacttca cggtcacccg
    31861 aatcctttcg cgatattgcg gctgagctga ccgcagcgtc aagcgcggat ttcaatcaga
    31921 aatgggtggt gggttacggg cattcatctg tggcagaaca tgccgtgctg cacatcgcgc
    31981 ttgagaatgt ctcgcggctg gcggttgaaa atatcgaatc caaccgtctg gcttcctata
    32041 cagaaaaatc tacccgctat caaacctggg aagatgacgc ctttcatcta cccgccgaat
    32101 tgaccggtca cccgttggaa ggcttgttca ccagcgcctg cgcgacgctc ttttccatct
    32161 atcgctcctg tattcaagcg gttatcgccc acctgaagag caattcccaa tcgcaggcag
    32221 gcgaaagcga agcacaactg aatcgccggt tacgctcaca ggcgattgac gcctgccgtt
    32281 ttttgcttcc agccgcctcg ctggccaatg tgggcgtaac catcaacgcg cgcgcgttag
    32341 aacacgcagt gggaaaactg ctcaccagtc cattgattga agcgcagcaa atcggcgccg
    32401 aaattcgcca tgccgcttct gcatccatcc ccaccctgct gaaatacgcg gaccagatcc
    32461 cgtatttaca gcatgtctgc cagcaatttt ctgccttatc gatccaggtg gatgccgccc
    32521 cagaacctga ttggtgccag ttgatcggtc cgcaacccga tgtgttagaa aaaatcctgg
    32581 cagctgagtt ataccgcgaa caggacatga gtttcacgca ggcattgaaa gccattcgta
    32641 cattgcctga ccatcaaaag aacacgttgt tgcgctgcct gctggagacg ccagaccgtc
    32701 ataccatccc ttcccgcgaa ctggaatacg cgtttttcac ttttgacctg ctgattgacc
    32761 agggtgccta ctttgaagtg aaacgtcacc gcatgatgac ccaaacagcg cagcttctca
    32821 ccgccaggtt cggctatgcc atccccgcgc ttatcactga ggcgggcatg gacgccgctt
    32881 attgcgctgc gatggaaaaa gcccgtgaag tttacgatca actggcagac ttcaaccctg
    32941 acgtggccgc ttacatcgtt ccaaatggct tcaaccgccg ggtactgctg caccttaacc
    33001 tgcgcagcct ggttcacttt attcgactgc gcagcgcccc caatgctcat ttctcagtgc
    33061 ggcgagtcgc gcagcgtatg gcagaagaaa tcagcgctca cttccccgaa ctgtctccct
    33121 tgctgggcag aaacgaaaac gagacattaa aaaacatcga aacaactttc tttaccaaca
    33181 ctcatgtggt aagttaagga aggagaacca tcatgtttat tccagaaact gagatcctca
    33241 tccggcttgg tcttgcggct ttgctgggta tgatggtggg gtacgagaga gagcgacaga
    33301 atcagccggc tgggttacgt acccatgtca ttctggcagt gggatcctgc cttgccatga
    33361 ccgtttcaat cgacctggcc atccagttct atccgcatgc gcccaacggg gaccctgcgc
    33421 gtctggcagc ccaggtgatt tcaggaatcg gtttccttgg ggcgggcgcc atcctgcgct
    33481 acgg
//
LOCUS       DAOTLW010000021        31838 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1681_length_31838_cov_9.696441, whole genome shotgun sequence.
ACCESSION   DAOTLW010000021 DAOTLW010000000
VERSION     DAOTLW010000021.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 31838)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31838)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31838
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1681_length_31838_cov_9.696441"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            54..1196
                     /locus_tag="P5516_04885"
     CDS             54..1196
                     /locus_tag="P5516_04885"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HRT91807.1"
                     /translation="MEDIKCNLHIHTIYSDGSGDYTQVASEALDAGVDVVIITDHNVL
                     IKGMERYVSKNGKRVLVLTGEEVHDQNRIPQKNHLLVLGCRREMAQHAYDPQCLIDQV
                     KRDGGLSFLAHPYEYDLPLFNETDISWESWEVEGFTGLELWNGFSELKTVARSLPDVI
                     FHAFFPDLIPHQPLPQALAKWDQLLAAGKKVVAVSGSDAHALHYRYKFIRKVIFPYRY
                     HFSSINNHLLLPSPLSGDLKKDKQAIYQSLKKGACYICLDRAAEPRGFSFTIENGETK
                     AHMGEYLFLDPGATIRVSSPRKARLRLICNGELLTEEDNQDLLVKTIEKPGAYRVEAY
                     LDHLGEQRGWIFSNPIYVMKKPKSIAVYTSDAIDKPNASSFNQTAQ"
     gene            1237..2016
                     /locus_tag="P5516_04890"
     CDS             1237..2016
                     /locus_tag="P5516_04890"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRT91808.1"
                     /translation="MSQLDKIEKHIRNIFESGAAILPWVGTDDQLLKHLSGAIHELFM
                     HEPPRRYLKPAVSILLNPRTASLWQSQADWECKLAELLTSSALEFGFRFHIAPKVTLV
                     PDPGIDIDETRITLIDQYQSERGETGVISLEAPTGFNEPLMNNPTLILQGNRTIELSQ
                     PVINIGRRSSNQIVINDLRVSRMHAQLRKVKDDYMIFDVGSTGGTFINSNRIDSHLLR
                     PGDVISLSGFIMIYTHDQSPTEEPQKSITSKIQNIEETEED"
     gene            2016..2447
                     /locus_tag="P5516_04895"
     CDS             2016..2447
                     /locus_tag="P5516_04895"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRT91809.1"
                     /translation="MLAVTILVLRVCLLLALLSFLGWSVYTLWWELKFQSESLAAQKI
                     PWISVFNDQEAADQKQVFTKPELQIGRDASCDIRINDETVSNQHARLSYRNKQWWIED
                     LLSTNGTFLNDERVETPTILINEDEIRVGKVLLTIEIQPLA"
     gene            2461..3330
                     /gene="mtnP"
                     /locus_tag="P5516_04900"
     CDS             2461..3330
                     /gene="mtnP"
                     /locus_tag="P5516_04900"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HRT91810.1"
                     /translation="MMNLKPVLAVIGGSGLYEIPSLEETQQVNPTTPFGSPSAPIVLG
                     NLHGKPVAFLARHGIGHTISPSQVNYRANIYALKMLGVERIIGVSACGSLREDFAPGS
                     IVIPHQLFDFTHKRARSFFDEELVVHVSSADPFCPELSTLLHQAISTTDGQVYQGGAM
                     ITIEGPRFSTKAESNIYRSWGLSLIGMTTSPEAFLAREAEICYSVMAHVTDYDVWHTT
                     EEAVSVEMVVKTLQRNTEIAQRSISTLVTMLPEVVDCSCSRALENAIITNRSFVSPKT
                     AEKLDLLIGKYIK"
     gene            3405..5960
                     /locus_tag="P5516_04905"
     CDS             3405..5960
                     /locus_tag="P5516_04905"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HRT91811.1"
                     /translation="MFLNSLVLTFAPAVRLHTLQAELRWQHWVGFATWLAGYAVLYRQ
                     LNKLLPERDPYLLPIIALLNGWGLLMIYRLSTNFGIRQTIWTALAIIGFLVALKYKNL
                     LPVLRRYKYVWLISGLLLTLLTFVIGTYPGGSGPGLWLNLGSVYIQPSEILKLLLIIY
                     LAAYLADTLKARLRLAQLLAPSLILIAIAVLILVAQRDLGTATLFIILYTIVVYLASG
                     KRRVLLISFIIVILALIAGYLVFNVIQLRIEAWLNPWQDARNNSYQIVQSLIAVANGG
                     LLGRGLGLGSPAVIPVAHSDFIFPAILEEFGVAGGLALVMVLALFTLRGLTIALRAPN
                     QFQRFLAAGLTSYIATQSILIMGGTIRLLPLTGVTLPFISYGGTSLVVSAASALLLMI
                     ISNQPKDQAAPIDRTRPYKLVGGVFLAGFAAITMLGIYWGFFRADALLARGDNPRRAI
                     SDMYVYRGTLLDRNNHPLTANSGLAGKYKRDYLYPPLSAVIGYSDPNYGQTGIEFRMD
                     DYLRGLAENSRFHVDSVRLLYGQDPAGFDLRLSLDLPLQQITDIAMAPYTGAVVLLNA
                     ESGEIIVMSTSPTFNANELEEKWQTWMEDKNAPLFNRATQALYPPGGVTGGILLARFL
                     AEYTPSSAVPDYPWNTTAEDEFTCAIQPPANPNWGDLVSSGCVKALSTISRSTLLGDT
                     LKLYKDVGFDQELLLPLEVSVPILPESVASYSDLFSGKAGILLSPAQVAVAVASLSNS
                     GRVIAPRIATAFSPNQIDWHLFEQGQAPRILPNFEPELAATMLTSGNYPGWEVSASAA
                     YQGTSVSWYAAGTPSDWLGAPLTIVVALENTPAVTAREIGRTVLLSATASLSE"
     gene            complement(6009..7223)
                     /gene="tyrS"
                     /locus_tag="P5516_04910"
     CDS             complement(6009..7223)
                     /gene="tyrS"
                     /locus_tag="P5516_04910"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HRT91812.1"
                     /translation="MNLNEQVDYLMQGTEYGDETLKQKMADELRNRLHTCQKEGRALR
                     VYCGFDPTKADLHLGHTVPMRKLRQFQELGHEVTFLIGNYTSLIGDPSDKDKLRPQLS
                     PEEIAANAQTYAEQAFRIMEEGKTRIRYNAEWLAGLSFEDLIRLGSNFTLQQFLTREN
                     FKLRWEKGDPVFLHETFYSLMQGYDAYMLNADVQIGGTDQLFNIMTAARKIMEFLGAQ
                     PNIAIILGILPGTDGVVKMSKSLGNHVPINTDAADMYGKIMSIPDSAMPVYYRLVTPL
                     TPPEIKHIEDGVRTGSMHPRDAKMRLAREITAAFYGAEEAQKAEKSFVDTFQKGGIPE
                     ELQVMKVSPSNTILEILLLTNLTKSKSEARRLIEQKGVRFDGELIEDPSATPAKEGVL
                     QAGKRHFVRIVF"
     gene            7470..7895
                     /locus_tag="P5516_04915"
     CDS             7470..7895
                     /locus_tag="P5516_04915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HRT91813.1"
                     /translation="MLTDKATLILDGRGWIHTTQNAYPIPSVIRLSRMVHPPRPKVTL
                     SRREIFRRDHYTCQYCGKSNTTLTIDHVIPKHLGGAHDWSNLVTACSFCNHQKGGRAL
                     SETGMSLMRQPKEPPQSALYIFARYLHENVEWNEFLEGW"
     gene            complement(7897..8640)
                     /locus_tag="P5516_04920"
     CDS             complement(7897..8640)
                     /locus_tag="P5516_04920"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT91814.1"
                     /translation="MSVVTILGSANAVPKIGQDNTHLLIQANHKTVMVDCGDNPVAKL
                     AAAGSHINQVNDLILTHFHADHMGSLPLLIMDMWLEKRESPLTIHGLEITLSKARSLL
                     EVFNWQDWKGMFPVNFNVIPDAGHEGFILDEDLAISTLMVQHLVPTIGLRIEIMGKKV
                     IAYSCDTEPCENVLKLASQADVLLQEAAGEGKGHTSPAQAGWLATRAGVKKLVLIHYD
                     SRIDEERLLADARSEFTGEVVLAKDMMLV"
     gene            complement(8660..9478)
                     /gene="lgt"
                     /locus_tag="P5516_04925"
     CDS             complement(8660..9478)
                     /gene="lgt"
                     /locus_tag="P5516_04925"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:TIGR00544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HRT91815.1"
                     /translation="MINGFTIFGIKIYFYALIIIAGALLGAVVSSRELRRRGLDKDII
                     WDLLPWVLIAGIIGARIWHVLTPDSTMLIDGKNPYFMRPIEMLNIRNGGLGIPGAVMA
                     GVLALWFYCRKKKLAFSRLLDVIAPGLALGQAIGRWGNFFNQEVYGLPSNLPWAIKID
                     EAHRLPGYANIERYHPTFLYESIWNILNMLVLLWIARKFESKLKPGDVFLVYLIIYPF
                     GRFLLEFIRIVHSPIAGINSNQWLMAVIAVAAAITLLVRHLPKKPAVNPNPTDD"
     gene            complement(9489..10127)
                     /locus_tag="P5516_04930"
     CDS             complement(9489..10127)
                     /locus_tag="P5516_04930"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HRT91816.1"
                     /translation="MEEKHTEPSAKSTWSLEIFLRKLFKGVIDPIASLFIKMGLKPNT
                     ITAMGFILTILAAYFTATGRFLIAGLILLVGAPLDVVDGSMARLRGEPTRFGGFIDSV
                     TDRYSELVLLLGLLFYYVQQQSLSACLLTFIAAAGSVMVSYTKARAESLGFTAKVGLL
                     TRVERMIIMVAFLLIKRPIIALWIISIGANITAIQRMWFVYKQSKEQVNTGT"
     gene            complement(10192..12369)
                     /locus_tag="P5516_04935"
     CDS             complement(10192..12369)
                     /locus_tag="P5516_04935"
                     /inference="COORDINATES: protein motif:HMM:NF013727.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HRT91817.1"
                     /translation="MTPRPSPKDKFPPKKNPAPKEFVPLVERIDAILTHFRQFGWDLL
                     GTFLILLAILTSLGLLGLSRGTLINSWITLIRRAFGLGSYLLVLVLVFVGVSVFRHAA
                     GKRFPLSIGKVLAIEGMLLVLLPILAFTGNVSIERAYDGLDGGTFGWGLAQLLIPLIS
                     KPLTLLFYLFLFFVFAGLSTGFFPRVLGKARAWSRGETAHQQASGRAPATKISKSEKP
                     GSARKAAGEKLEKTEPSSLVSWRDAALPPMSLLLNDQIIPPDQVLIKDNARLIEEKLA
                     EFGVPVKVLGFRVGPAVTQYAVEPGYTERKGADGVVTRQKVKVAKITSLQDDLKLALS
                     AQRLRIESPVPGRSYVGIEVPNEDNLFVRLRPILESETFRKKKSPLLLALGRDVSGEP
                     IQVDLEKLPHLLIAGTTNSGKSVCVTALAACLAMNNTPDNLRLVMLDPKMVELVRFNG
                     LPHLIGKVETEIDRMLAVLRWAQMEMDSRYRRMAEVHARNLDTFNEKMLQKKRTPLPR
                     IVIFIDELADLMINAPEQTEHSLIRLAQMARATGMHLVVATQRPSTDVVTGLIKANFP
                     ARISFAMASAVDSRVILDANGAETLLGKGDMLLLDPARSGLQRLQGVIVSDGEIEKII
                     EYWQSAVHEEDGAPPWEGLVDNRENEGFDALLERATTIVRETGKASTSLLQRRLRIGF
                     PRAARLIDELEEMGVIGPAVGSGKEREVLEVEEEEPESEEDEA"
     gene            complement(12366..13313)
                     /locus_tag="P5516_04940"
     CDS             complement(12366..13313)
                     /locus_tag="P5516_04940"
                     /inference="COORDINATES: protein motif:HMM:NF012784.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HRT91818.1"
                     /translation="MVEIVQERKQEASIPEMDEGWWASVLADEEKFFRENKEVNCKPC
                     ESKTTSQLDWDCIKAVYADDEVVLLEVQGYNRGGLLVQGAGIQGFVPVSHLVDFQAEV
                     SDEVRQQKLANYVGTSLYLKIIECDMESERVVLSERAAQAGEGKRKELFTQLTEGTIV
                     CGVVTNITDFGVFVDLGGVEGLIHVSELSWGRVEKPSHVFKVGEQVKVIVLQVNEANS
                     RIALSHKRLTPNPWERLSKIYKPGDVVSAELTSIMRFGIFARLDEGVEGLIHISAFPE
                     TLRKMNIAEHYTPGQRVQVKILHVDADRRRLGLGLISEE"
     gene            complement(13357..15261)
                     /locus_tag="P5516_04945"
     CDS             complement(13357..15261)
                     /locus_tag="P5516_04945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91819.1"
                     /translation="MMVSNWVNEIIAIARTISEILTAGIAITAFALLLYSLTFNLLER
                     VARTFATIMVCLVIVFSTESFGATSKDPYMIDFWLRAQWVGIILLPAAYLNFSDALLA
                     TTGRPSRWRRRWAIRATYLFSFILIFFLFTPWFLGELVLDQPPAPHHLPTIYTQLFIV
                     YYIIILVLAWVNFRRAYQRTLTRTSKRRMLYLAISAIGPVVGAFPFLLYSSEFASQWT
                     LLFWLLAATVSIGLDICIFIMAYAVAFFGVPWPDRVVKSRLIKWTLRGPITAVLTLGL
                     MTLVKRANIALGTPYDAFVPIVMVVTVLICEYAITLFFPAFERWLIYDRDEQDLRFVL
                     TLEQRIITRGDLKQFIEMVLAAVNDRAQAQGAYLLEKNDEGFTLVEKTPDFSFDPQLL
                     EELDYLESAGNIHEEYVECRSDILIPLHNNSNGSGEHLLGYIGITGIKDISALAAEDQ
                     AALKRLAARAAIALRDRHMLEQVFDTLERLEPQEELIQKLRAVSRFDQRGMLMEADWM
                     EKKEMTTWVKGALTHYWGGPKLTDSPLLKLEIVKEAMAKHDNNEVNALRTVLRSALEQ
                     LKPEGERKYTGEWLLYNILEMKFLEGKKVREIAVKLAVSEADLYRKQKVAIEKIADRL
                     VQMELNTRNT"
     gene            complement(15266..15805)
                     /locus_tag="P5516_04950"
     CDS             complement(15266..15805)
                     /locus_tag="P5516_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT91820.1"
                     /translation="MAEVEIRPAVSSDISTLEKFDHTIETTHVWRVDGSTTKDRIAIE
                     LNEVKLPRVLRLNYPRRAELLRNTWTRHLLFLIASCEGSLAGYLTLDEDNDKLLAVVH
                     DLVVNTPFRRQGIASALVFSARDWVKKRGMTRMLLEIPAKNHAMVEMAVKLRFSHAGQ
                     VDNFYQNGDMVLFYLSELK"
     gene            complement(15808..16494)
                     /locus_tag="P5516_04955"
     CDS             complement(15808..16494)
                     /locus_tag="P5516_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91821.1"
                     /translation="MSSILRFLEKYEILIFLVAGLIILASARKTYVAWREWSRSIFGL
                     EKEHAQQIFNSALTVLVFSVLLATGLFLVNTFITPAVPGVQQLPTPTIDLTALPPTET
                     PAPILMVTGEGLIPTITSYFSRGCIPDQIDWTDPKNGDTLSGRVELKGTVNIENLGFY
                     KYEYSPSDSDTWTTIAAGNAKIINEPFGGTWDTRALIPGEYKLRLVVNDIQNNSLPAC
                     VILVTIKASE"
     gene            complement(16533..17378)
                     /locus_tag="P5516_04960"
     CDS             complement(16533..17378)
                     /locus_tag="P5516_04960"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HRT91822.1"
                     /translation="MDSFITIREIDSAANFITRQIVEKPVVALVLGSGLGGLAEAVND
                     ARIIPFNQIPEWPVSTIQGHSGRLVFGRLFGVPAMVMQGRVHYYEGYSMDRVTFPIRV
                     MQRMGVRNLVLTNAAGAINPDYVPGDVMLLTDHLNLIGMAGQNPLRGPNLNEFGERFP
                     DMSQPYDSLFIAAARQVAQKHHIKLHEGVYVCLAGPSFESPADLRFLKIIGADAVGMS
                     TVPENIVARHAKMRVLGLSGISNKANLDGSTVTTHEEVLEAGKVIVPKLTTIIRGVIE
                     TLAGE"
     gene            complement(17457..18500)
                     /gene="ltaE"
                     /locus_tag="P5516_04965"
     CDS             complement(17457..18500)
                     /gene="ltaE"
                     /locus_tag="P5516_04965"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HRT91823.1"
                     /translation="MKLIDLRSDTVTTPTEEMRQAMAATEVGDDVYGEDPTVNRLQEM
                     SAALLGKEAGLFVPSGTMGNLLSVLSVCNRGDEVIMGNLGHTFLYEAGGISALGGIFA
                     HTLPNQADGSLMLEDIENAIRPDNIHDPITRMVILENTHNRCGGTVLTERYTREVAEL
                     AHRNHLHLHLDGARIFNAAAALNVDVKALTEPVDSVTFCLSKGLCAPVGSVVCGTKEF
                     IKKGLRVRKMLGGGMRQAGIIAAAGIFALENMITRLREDHQRATLLAEGLMTVPGIHF
                     EIGMPQSNMVFLSLKEEVIFSAQEVAERLARLGVKVGVVAQRRFRLVTHYWITDKDVD
                     ETIRAFRSALSGA"
     gene            complement(18514..19431)
                     /locus_tag="P5516_04970"
     CDS             complement(18514..19431)
                     /locus_tag="P5516_04970"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HRT91824.1"
                     /translation="MSSQRCQFIVQSGDENRLDKFLARQLPGQSRSRLQAIIKDGGVT
                     VNETVATKSGLALLTGDKVSICIPEVVPVNLEPESIPLDIVFENEHLVIINKPAGMVV
                     HPAPGHASGTLVHAALGNFGDLQGIGGEVRPGIVHRLDKDTSGLIIVAKNGKAHRWLQ
                     EQFRLRKVKKTYLALVDGKPPTPSGRVEAAIARDAAHRKQMTVVSDGRGRAAITEFHT
                     VKQFNQHTLVEAHPLTGRTHQIRLHMAFLGCPIVGDLVYGRKKPSLDLERHFLHAYQI
                     SIILPGESEARTFQAELPADLQTILDQLI"
     gene            complement(19428..19955)
                     /gene="lspA"
                     /locus_tag="P5516_04975"
     CDS             complement(19428..19955)
                     /gene="lspA"
                     /locus_tag="P5516_04975"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HRT91825.1"
                     /translation="MKHFLKKTVWLFVVAASIVTLDQVLKKIVITHLPYGEVWSPWEW
                     LTPYARIVHWSNTGVAFGMLQGMNPVFIGLAVLVSFGIVYYYPKIEKKDWMIKLALMM
                     ELGGAVGNLVDRIKYGHVIDFISVGTFPVWNIADACITVGVIILLLGVWLQEKREKNG
                     KAKTTQEQADNEVSP"
     gene            complement(20039..23188)
                     /gene="ileS"
                     /locus_tag="P5516_04980"
     CDS             complement(20039..23188)
                     /gene="ileS"
                     /locus_tag="P5516_04980"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HRT91826.1"
                     /translation="MFKPVSPKLDITDMELKVLRYWQTHEIFHKSMSSREGGPEYVFY
                     EGPPTANGKPGVHHVLARAFKDIFPRYKTMRGYHVSRRGGWDTHGLPVEIEVEKKLGF
                     TNKQQIEDYGIGKFNDLCRESAFTYIQEWEKLTDRIGYWVDLTNAYITYTNEYIESVW
                     WILKSFWEKNLLYQGYKVVPYCPRCGTPLSDHEVAQGYRDAPDPSVFVRMPLVDTPGT
                     SLLVWTTTPWTLPANVAVAAGAEIDYVTVERQMEDGSKEELILAESLVERVFAGETVQ
                     VMNRFKGKRLQGLRYQPLFTFLPAEKKAHYVVLGEFVTTEDGTGLVHIAPAFGADDMQ
                     VALEHDLPILNTVAQDGTFIPQVNPWAGIFVKDADPQITQNLKNRGLLFREETYLHTY
                     PFCWRCDTPLLYYARPTWYIRTSQYKERMVKLNEEINWYPEHIRKGRFGNWLENNVDW
                     ALGRERYWGTPLPVWQCDTCHQQECIGSIAELSDRAGRNLANANLHRPEVDKIQFACS
                     ACGGNMRRVPELIDVWFDSGSMPVSQWHYPFENVEAFKSQFPADFICEAVDQTRGWFY
                     SLHAISTLLFDEKCFKNVICLGLILDGEGQKMSKSRRNVIAPWSVIEKHGADAMRWYL
                     YTASPPGQERRFSADLVGEVLRNFTLTLWNTYSFFVTYANLDKWSPVPDEKVAYSALD
                     KWMRSRLHALIRDVTIALENYDVLGATRPVQTFVEQLSNWYLRRSRRRFWKSESDADK
                     NAAYSTLYECLTALSKLLAPTMPFIADELYLNLVGSVDKKAWESVHLAPWPEFDSETI
                     DEKLNDEMELVMKLTSLGHAARNKANRKVRQPLAEVAFSVGSQEEERVIKRNAELLAD
                     ELNVKKVRTLNAAGEAVSYTLNPLPKQLGQKYGSLYPEIRKKVLALDVEASALKLLAG
                     ESLRIEAGGNVLDILPEEVEVRLQAKEGYSVASEGAYLAALVTELTPDLISEGLAREF
                     VRRVQDLRKTADLDIADRIRLYYSATPLLAQAVETYRDYIMMETLAVAFQGGGAPEGT
                     FTVTDEFDQEKVTIHIIKA"
     gene            complement(23504..24814)
                     /locus_tag="P5516_04985"
     CDS             complement(23504..24814)
                     /locus_tag="P5516_04985"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HRT91827.1"
                     /translation="MRAVDIIIKKRDRKEMTREEIEFFVRGIVNGEIPDYQVSAWAMA
                     VLLNGMSDQETTDLTLAMAYSGEVLDLSGIVPMEVDKHSTGGVGDKTTLVVEPLVSAC
                     GLPVGKMSGRGLGFTGGTLDKIESIPGMRTNLTQEEFKRLLRDEGLVLAGQSGDLAPA
                     DGKLYALRDVTGTVQSIPLIASSVMSKKIAGGAQGIVLDVKVGSGAFMKNLEEARQLS
                     RLMVTIGTLSGRKVVALLSDMNQPLGRAVGNALELVEAIDTLKGRGTADFTEHCLVVA
                     SHMLLIGEKAQNLDAARKMAEAALADGSGFEKLKQLVKNQGGDAAYVDDCALFPPAPI
                     IRGVQSPQKGYLSRVDALEIGEASVNMGAGRNKKEDTIDHRVGIIVHHKVGDLVHTGD
                     ELFTIHARDESSWQMAANSVLSACRWQEQPCEPLPLFYEVIKQK"
     gene            complement(24917..25693)
                     /gene="jag"
                     /locus_tag="P5516_04990"
     CDS             complement(24917..25693)
                     /gene="jag"
                     /locus_tag="P5516_04990"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_process: GO:0051510 - regulation of unidimensional cell
                     growth [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HRT91828.1"
                     /translation="MSTEKTTLEIIAPTTEEAVERGLEQLGLTEDMVEIEVLDGGSRG
                     LLGLGGRQSRVRLTVKSFETLIDEHEPIFTGDADSEEITIEPSSVHAIDLADLSEEDQ
                     QNVKSVRSVVIELLERMRVKADVSARYIEPQDEKDQRVVLVDVEGKDLSILIGRRSET
                     LNALQYITGLIVSKELGRWVPLLIDIQGYRSRRERQLRQLGRRMADQAVQTGRKQTLE
                     PMPANERRIIHLELRDHPQVFTESVGEEPYRKVTIALRNP"
     gene            complement(25722..26504)
                     /locus_tag="P5516_04995"
     CDS             complement(25722..26504)
                     /locus_tag="P5516_04995"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HRT91829.1"
                     /translation="MWDTIIIEPFINLLLWISKLVGNFGLALILFTILTRIVLYPLTA
                     KQIKSSAAMQELQKDKRYIEIQTKYKGDKEKLAQEQMKLYQEMKINPMASCLPTLIQF
                     PIIIGLYQAMIQTMATGPLDLLNLTRHIYPKFLSIAEVLPLNSKFLWMDIGMPERLHI
                     PGLNFGIPVLAIVVVISTYISTKIMTPPQQPGAADQGAMMSKMMNLYMPVLMGWLAWS
                     LQSGLALYFVVTNFVQIAQYAISGKVHWDNVVFWKKKPEGKK"
     gene            complement(26517..27572)
                     /locus_tag="P5516_05000"
     CDS             complement(26517..27572)
                     /locus_tag="P5516_05000"
                     /inference="COORDINATES: protein motif:HMM:NF024753.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-like beta-propeller repeat protein"
                     /protein_id="HRT91830.1"
                     /translation="MKIRRIGLILLFVLLAVTLTACSRAGQSNSWSGVLVTEDYVYYA
                     GGSQVTALVADSGNVAWQYPEKGSPQRLFYAEPVLIGEQLIVVDYAHQLTCLNAKTGA
                     ETWKFTQAEGRYIDSPLVVNNLILAPNTDYSLYALDLSGNLVWEFKAGHALWARPATD
                     GTTVYFPSMDKYLYALDAATGALKWKVALDTTTISRPLLENGVLYLGNLDGTFFAFNA
                     EDGTRIWQQKVGGGVWSQPVLHDGKLYFGDQSGRINILNSENGKSVQQIETDAAIVGT
                     GALLDEGLVFGNEDGQVILVGFNGERLWTRSFDGSIYASPERLGNRLVISLNKSEKPL
                     IALDTNGNENWYYGKKK"
     gene            complement(27618..27824)
                     /gene="yidD"
                     /locus_tag="P5516_05005"
     CDS             complement(27618..27824)
                     /gene="yidD"
                     /locus_tag="P5516_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013932.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HRT91831.1"
                     /translation="MPLLGLIRLYQWLVSPALPANTCRFYPSCSHYGYQAIYKHGAIK
                     GTILAVWRVLRCNPFNPGGFDPVP"
     gene            complement(27889..28248)
                     /gene="rnpA"
                     /locus_tag="P5516_05010"
     CDS             complement(27889..28248)
                     /gene="rnpA"
                     /locus_tag="P5516_05010"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HRT91832.1"
                     /translation="MKRKFRLTQTNDFKRVRHTGRSYAHPLAVLVCAKGLAENSRAGI
                     IATRSVGGAVERNRVKRQLRAIIAPYLSELKDPIDFLLIARERTSQVQFQEIQAAVVE
                     LLGKAGLIGSNNYRPGR"
     gene            complement(28315..28485)
                     /gene="rpmH"
                     /locus_tag="P5516_05015"
     CDS             complement(28315..28485)
                     /gene="rpmH"
                     /locus_tag="P5516_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001686914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HRT91833.1"
                     /translation="MTKRTYQPKIRRRVRVHGFRARMATADGRATLKRRRAKGRHHLT
                     VTMNNHVKKISW"
     gene            complement(28559..29239)
                     /locus_tag="P5516_05020"
     CDS             complement(28559..29239)
                     /locus_tag="P5516_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRT91834.1"
                     /translation="MFVVIAGGGRTGAQLAKSLVSAGHRVHLIESRKEILARIHKEIP
                     TESIIAGNHLDTHTMELADLIHADVFAATTPVDAENLALCYVARERYDVNRTIARVNN
                     PRNAWLFNKMFNVDVAVNQAEILAHLIEEEMSMGDMMLLLKLRRGKYSLVEEKIPVNA
                     TAVGMMIKDLKLPANCVIAGIIRDGELVIPRGVTTLAAEDELLVLTDAEGARMVAELL
                     DAPRVKNL"
     gene            complement(29241..29900)
                     /locus_tag="P5516_05025"
     CDS             complement(29241..29900)
                     /locus_tag="P5516_05025"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HRT91835.1"
                     /translation="MKIIVIGCGRIGADLAYNLFKRGHQVSVIDENAASFKNLPSDFE
                     GKTLEGDAINQDLLTRAGIQTCDALAAVTNNDALNVVVSHVAKNEFNVKNVVARNYDT
                     HLRSIFETFNIQVISSSSWGAQRIEELLLDAEGRTVFSAGNGEVEIYEIIIPPSWNEK
                     KVSDLVTCSKCKWLAVTRAGSAFLPDQDTVIKAGDILTVAATFEGITETRENLRKRQV
                     S"
     gene            complement(29916..>31838)
                     /locus_tag="P5516_05030"
     CDS             complement(29916..>31838)
                     /locus_tag="P5516_05030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRT91836.1"
                     /translation="STVFRRATEYQPGRSWSRFLIGRPLSTADAPHQTIGKVLGLAVF
                     SSDAMSSVAYAPQEILIILAAAGVTAFHLSVPIAIGICALLAILIFSYEQTVHAYPNG
                     GGSFIVARDNIGAIPAQIAAAALLTDYILTVSVSISSGVAQLISAFPLFEPYKVLIAV
                     SFVLLIMLVNLRGVKESGITFAIPTYFFLGMMYINVIVGFIRLIAGTLGSVEAPPMDL
                     ILPESALQPVTLFLVLKAFASGTSAVTGVEAISDGITAFKEPRSKNAGTTLIIMALIL
                     GSMMFSITFLGNHVGVMPSEEETVISQMSRTIFEGRGLLYLGTIAATTIILVMAANTA
                     FADFPRLSAIAAADGYLPRQLTYRGSRLVYSRGIAALAIVASLLIIIFQASVSRLIPL
                     YAIGVFLSFTISQSSMARRWWKSGKLEPGVEVQERGSLLKYDSGWKHKMLINGFGALI
                     TFVVMIVFAVTKFVDGAYIVVFLIPALVLGFSTIHKHYYTLAKDLSLEEYAPPGRIVH
                     QRVLLLVSGVHKGTLTALRYAKTLSDDVTAVHVSIDPLETRRMLEKWEMWGEGYRMVV
                     IESPFRLFIEPLLEYIEEIDKNRPPNEIISIIVPQFIPRRGIASLLHSRTADLLRKVL
                     LSRKDIVIMEVPYLVD"
BASE COUNT         7658 a         8757 c         7355 g         8068 t
ORIGIN      
        1 gaaatttcaa acaccgcaga ttataccgtt cataaccgaa aatcgtcaac ctaatggaag
       61 atatcaaatg taacctgcat attcacacga tctactccga cgggagtggt gattacaccc
      121 aggtggcttc agaagcgctg gatgctgggg tagatgtggt gattattaca gaccacaatg
      181 ttctaataaa agggatggag aggtatgtat cgaagaacgg gaagcgagtg ttggtgctca
      241 ccggcgaaga agtccacgac cagaatcgaa ttccgcagaa aaaccacttg ctggtgctcg
      301 gctgccggcg cgagatggcg caacacgcat acgaccctca gtgcttgatt gaccaggtga
      361 agcgcgatgg tggcttgtcg ttcctggctc atccttacga atacgacctt ccattattca
      421 atgaaacgga tatctcttgg gaatcctggg aggttgaagg gtttaccggc ttagaattat
      481 ggaacggctt tagtgagctt aaaacagttg cgcgcagcct gccagatgtg atctttcacg
      541 ccttctttcc tgacctcatc ccgcaccaac ccttgccgca ggccctcgcc aaatgggatc
      601 aactcctcgc tgccggaaaa aaagtagtgg cagtatcagg atctgatgct catgcgctgc
      661 actatcgtta caaattcatc cgaaaggtga tatttcctta ccgttatcac ttttcatcga
      721 tcaacaatca cctccttctt ccttcaccgt tgagcggcga tttaaaaaag gacaaacagg
      781 ccatctacca gtcgcttaag aaaggcgcct gctacatatg tctggatcgc gccgctgaac
      841 cgcggggctt tagcttcacc atcgaaaacg gggaaaccaa ggcgcatatg ggagagtacc
      901 tcttccttga ccctggggca accatccggg tcagttcgcc gcgaaaagcc cggctgcggt
      961 tgatttgtaa tggtgaactg ctcacagaag aagataacca ggacctcctg gtaaagacaa
     1021 tcgaaaagcc cggggcttat cgtgttgaag cgtacctgga ccatttagga gaacaacgcg
     1081 gttggatttt cagcaatcca atttatgtga tgaaaaagcc aaaatccatc gcggtttaca
     1141 cttctgatgc cattgacaag ccaaacgcat cctccttcaa ccagaccgcg cagtgaggtt
     1201 ggttaaactc ggctgtcgtg gttataatca atatcaatga gtcaactgga taaaatcgag
     1261 aaacacattc gcaatatatt cgaaagcgga gctgccattc tgccctgggt gggaaccgac
     1321 gatcagctgc tgaagcacct cagtggagca attcacgagc tctttatgca cgaaccacca
     1381 cggcgttatt taaaacccgc cgtgagcatt ttgttaaatc cccgcacggc ttccttatgg
     1441 caatcacaag cagattggga gtgtaaactg gcggagctgc tcacatcatc agcgcttgag
     1501 ttcggttttc gctttcacat cgcccccaag gttacgcttg ttccagaccc cggaatcgac
     1561 attgatgaaa ccaggatcac cctgattgac cagtatcaga gtgagagagg ggaaacaggg
     1621 gtaatctcct tggaagcccc cactggtttc aatgagccgt taatgaataa ccccacgctc
     1681 attctccagg gaaacagaac catcgagtta tctcaaccgg tgatcaatat cggccggaga
     1741 agcagcaatc agatcgtgat taatgacctg cgggtttctc gtatgcacgc tcagctgcga
     1801 aaagtcaagg atgattacat gatttttgat gtcggctcaa caggcggcac attcatcaat
     1861 tcaaaccgta tcgacagtca ccttctgcgc ccaggggatg taatttcttt gtccggcttc
     1921 atcatgattt acacgcacga ccagagccca accgaagaac cccaaaagag tatcacttca
     1981 aaaattcaga acattgaaga gactgaggag gattaatgct ggcagtcacc attctcgtcc
     2041 tgcgggtttg cctcctcctc gctctgcttt ccttcctggg ctggtcggtt tacaccttat
     2101 ggtgggaatt aaaatttcag agcgagagcc tggctgcgca aaaaatcccc tggatttctg
     2161 tgtttaacga tcaggaagcg gccgaccaaa aacaggtgtt taccaaaccc gaattacaga
     2221 ttgggcgtga tgcatcttgt gatatccgta tcaatgatga gacagtttcc aatcaacacg
     2281 cacggttatc ctatcgaaat aaacaatggt ggatagagga ccttttatcc acaaacggaa
     2341 cttttttgaa tgacgaacgg gtcgaaactc cgacgatctt gattaatgaa gacgagataa
     2401 gagtcgggaa agtactgttg accattgaaa ttcaacctct cgcataaggc agaaggagcc
     2461 atgatgaatt taaaacctgt attagcagta attggaggat ctggtcttta tgaaattcca
     2521 agtttggaag aaacccaaca agtcaacccc actactccct ttggttctcc cagcgcccca
     2581 atcgtcttag gaaacctgca cggtaagcca gtggcttttc tggcgcggca cgggattggc
     2641 cacaccatct ctccttcgca ggtaaattac cgcgccaata tttatgcctt gaagatgctt
     2701 ggtgtggagc ggattatcgg tgtaagcgcc tgtggctctc ttcgcgagga tttcgctccg
     2761 gggagtatcg tcattcccca tcagttgttt gattttactc ataagcgcgc ccgctccttt
     2821 ttcgatgaag aactcgtggt gcacgtcagc agcgcggacc ctttttgccc cgaactttcc
     2881 acattgctgc atcaggcgat atctaccact gatggccagg tgtaccaagg cggggcaatg
     2941 atcacaatcg aaggaccgcg tttctcaaca aaagcggaat ccaatattta ccgctcatgg
     3001 gggttgtctc tcatcggaat gaccacctct ccagaagcgt tccttgcccg tgaagccgaa
     3061 atttgttatt cggtgatggc ccatgtaact gattatgatg tatggcacac caccgaagaa
     3121 gccgtcagtg ttgagatggt ggtcaagaca ctgcaacgca atacggaaat tgcccaacgt
     3181 agtatatcca cgcttgtgac catgctgcct gaggtcgtcg attgcagttg ttcacgggca
     3241 cttgagaacg caatcatcac caaccgttcc ttcgtttcac caaagaccgc cgaaaagttg
     3301 gatctattga ttggcaaata tatcaagtga gactatcgtt ttcaaataac cgcccatcgc
     3361 agagtcacgt tcagaccagg ctcatgttgc tggtgctggt ttttatgttt ttaaacagcc
     3421 tggtgttgac ctttgcccca gcggtaaggc tacacaccct tcaggcagag cttcgctggc
     3481 agcattgggt gggttttgct acctggctgg ctgggtacgc ggtgctttac cgccaactga
     3541 acaaattatt gcctgagcgt gacccatacc tgctgccaat cattgccctg ctaaacggct
     3601 ggggtctctt aatgatctac cgtctatcca ccaactttgg tattcggcaa acgatctgga
     3661 ctgcgcttgc gattatcggt ttcctggttg cgcttaaata caaaaacctt cttcccgttt
     3721 tgcggcgcta caagtatgtt tggctcatca gcgggctgtt gctcaccttg ctcacatttg
     3781 tgatcggtac ttaccccggt ggaagtggtc ctgggttatg gttgaacctt ggaagcgtct
     3841 acattcaacc ttcagaaatc ttgaagctcc tgttaattat ttacctggca gcataccttg
     3901 ctgatacatt gaaggcgcgc ttgcgcttgg cccaattact ggcgccctca ttgatcctca
     3961 tcgccatcgc ggtgctgatt ttagtcgccc agcgtgacct gggaaccgca acccttttta
     4021 tcattcttta cacgattgtg gtttatctcg cttctggcaa acggcgcgtc ctcctcatta
     4081 gctttatcat cgtcattctc gcgcttatag cgggatacct ggtttttaac gtgatccaat
     4141 tacgcattga agcctggctt aacccgtggc aagacgcacg caacaattca taccagatcg
     4201 tccaatctct tatcgctgtc gccaatggtg ggttacttgg ccgcggatta ggactgggca
     4261 gcccggccgt catccccgtg gcacattccg acttcatttt tcccgccatc ctggaagagt
     4321 ttggtgtagc cgggggattg gcattggtga tggttctcgc cttatttacc cttcgcggat
     4381 taaccatcgc gctgcgcgcg cccaatcagt ttcaacgctt tctggctgcc ggtctcacat
     4441 cttatatcgc cacacaatcc attcttatta tgggtggaac cattcgcctg ctcccgctta
     4501 caggcgtcac gctgccattc atctcctacg ggggaacttc actggtcgtc tccgcagcat
     4561 ctgccctttt attgatgatt attagcaacc agcccaaaga ccaggcggct ccaattgata
     4621 gaacccgtcc ttacaagctc gttggaggtg tttttctggc tggttttgca gcgatcacga
     4681 tgcttggaat ttactggggc tttttccgcg cggacgcctt gctggcgcgt ggagacaacc
     4741 ctcgtcgcgc catttcagac atgtacgtat accgcggtac cctcctggat cgtaacaatc
     4801 acccgctcac cgctaacagt gggcttgccg gtaaatataa aagagattat ctctatccac
     4861 cattgagcgc agtgatcgga tatagcgacc cgaattatgg tcaaacgggg atagagttca
     4921 ggatggatga ctacctgcgc ggtcttgctg aaaactcccg ttttcatgtg gattctgtgc
     4981 ggttactcta cgggcaagac cccgcggggt ttgacctcag gttaagcctg gatctgcctt
     5041 tacagcaaat tacagacata gcgatggctc cctataccgg agcggttgtg ctcttgaacg
     5101 ctgaaagcgg tgaaattatt gtcatgtcaa cttcgccgac ttttaatgcc aacgaactgg
     5161 aagagaaatg gcaaacctgg atggaggata aaaatgcccc cctcttcaac cgggcgaccc
     5221 aggcgcttta ccctccagga ggggtgactg gaggaatcct tttagcgcgt tttctcgccg
     5281 aatacacccc atcatccgcg gtgcctgatt acccctggaa cacaacggcg gaagatgaat
     5341 tcacttgcgc cattcaaccg ccagccaatc ccaactgggg tgatctggtc tcttccgggt
     5401 gtgtaaaagc gctcagtacc atcagccgct caaccctgct gggtgatacc ctcaagttat
     5461 acaaagacgt gggttttgat caggaattgt tactacccct cgaagtaagc gtacccatcc
     5521 tgcctgaatc agtcgccagc tattctgacc ttttttctgg aaaggcaggg attctgcttt
     5581 caccagcgca ggtagcggta gcggtagcca gtctttccaa tagcggcagg gttattgccc
     5641 ctcgtattgc cacggcgttt tctccaaacc aaatagattg gcaccttttc gaacaagggc
     5701 aagcgccgag gattttaccg aactttgagc cagaactggc ggcgaccatg cttacttcgg
     5761 gtaattatcc cggttgggag gtttcagcca gcgcagcgta ccaaggcacc tccgtgtcct
     5821 ggtatgctgc cggcacacct tctgactggt taggcgcacc tttgaccatt gtagtggcgc
     5881 ttgaaaacac gccggcggta accgcacgcg aaataggcag aacagttcta ctctcagcca
     5941 cagcctcgct ctcggaataa aaaaaaactg gcagttcgaa ccctgccagt tttttgcttt
     6001 tactaagttt aaaagacgat ccgtacgaaa tggcgcttgc ctgcctgcag cacaccctct
     6061 tttgcaggtg tggcagaagg gtcctcgatc aattcaccat cgaaacgcac ccctttttgc
     6121 tcgatcagcc gcctggcttc acttttgctt ttcgtcaggt tggtgagaag cagtatctcc
     6181 agaatggtgt tggatggaga cactttcatt acctgcagtt cttccggaat accacccttc
     6241 tgaaaagtgt ctacaaacga cttttccgct ttttgagctt cctcagcccc gtaaaaagcc
     6301 gcggtgatct ctctggccaa acgcatcttg gcatctcgtg gatgcatgct tcccgtccgc
     6361 acaccgtcct ctatatgttt tatctcaggt ggggttaatg gggtcaccaa cctgtagtac
     6421 accggcatgg cgctatccgg tatgctcatt attttcccgt acatatccgc ggcatccgtg
     6481 ttgattggca catgattgcc cagggatttg ctcatcttca ccactccatc tgttccgggt
     6541 aaaatgccca ggatgatggc gatattcggt tgagcgccca ggaattccat gattttccgc
     6601 gccgcggtca taatattgaa aagctggtct gtcccgccta tttgaacatc tgcgttgagc
     6661 atatacgcat cataaccctg catcaaagaa taaaaagttt cgtgtaagaa taccgggtcg
     6721 cccttttccc aacgcagctt gaagttctcg cgggtcaaga attgctgaag ggtgaagtta
     6781 gacccaagac ggattaaatc ctcgaagctc agccctgcaa gccattcggc gttataccgg
     6841 atgcgggtct tcccttcttc cataatgcga aacgcctgtt cagcataggt ttgggcgttg
     6901 gcagcgattt cctcaggcga gagttgaggg cgcaatttat ccttgtctga agggtcaccg
     6961 attaatgaag tgtaattgcc aataagaaat gttacctcgt gtcccagttc ctggaactgc
     7021 cttagtttcc gcatcggtac ggtatgcccg aggtgcaaat cggcttttgt tggatcaaag
     7081 ccgcaataaa cgcgcagcgc tcttccttcc ttttggcagg tatggagccg atttcgcagc
     7141 tcatccgcca ttttttgttt cagtgtttcg tcgccgtatt ctgtgccctg catcagatag
     7201 tcgacttgtt cattgagatt cattgcggcc tcctttacat gatgttagca ggcatattat
     7261 accagcagta aatatgcttc cgctgatact tatcaggttg ctagtttatc gcctgcctgt
     7321 tggcaaccta aatgactaca cgaaagcatc tcaacccatt ttcatcgtgc tataatcatt
     7381 tttattatct tatggaaaac cctgtcctgg tgttaaactc caactttcag ccggtcaacg
     7441 tgtgtgattg tcaacgcgct gtttgtctca tgcttactga taaagccacc ctcatccttg
     7501 acggtcgtgg ttggatacac accacccaaa acgcttaccc catcccctcc gttatacgtt
     7561 tatcccgcat ggtccatcct cctcgcccga aagtgactct ttcacggcga gaaatttttc
     7621 ggcgtgacca ttacacctgc cagtattgtg gtaagtccaa taccacgctc acaattgacc
     7681 atgtaattcc caagcacctc ggcggtgcgc atgattggtc caacctggtt accgcttgct
     7741 cattctgcaa ccaccagaaa ggcgggcgcg cgctctcaga aactggcatg tcactgatga
     7801 gacagcccaa agaaccaccg caatccgcgc tgtatatttt tgcgcgttac ctgcatgaaa
     7861 atgtagagtg gaatgaattt ctggagggct ggtaggtcac accagcatca tatcctttgc
     7921 cagaaccact tccccggtga attcacttct ggcatccgcc agcaaccgct cttcatcaat
     7981 tcggctgtca taatgaatca gcacgagttt tttcaccccg gcacgcgtag ccaaccagcc
     8041 tgcctgggct ggtgatgtgt gccccttgcc ctctcccgcg gcttcctgta gaagtacatc
     8101 tgcctggctg gccagtttca acacgttttc gcagggttcc gtatcacagg aatacgcaat
     8161 gaccttcttc cccattatct ctattcgcaa accgatagtc ggaacaaggt gctgcaccat
     8221 caaggtgctg atggccaggt cttcatccaa aatgaaccct tcatgacccg cgtcagggat
     8281 cacgttgaaa ttcaccggga acatcccctt ccaatcctgc cagttgaaca cttcgagcag
     8341 cgacctggct ttgcttaaag ttatctccaa tccgtgaatc gttaatggag attctctttt
     8401 ttccaaccac atgtccataa tcaacagcgg caggctgccc atatggtccg cgtggaagtg
     8461 agtcaaaatg aggtcattga cctggttaat atgagaacca gcggcagcca gcttggccac
     8521 gggattgtcc ccgcaatcga ccatcaccgt tttatgattt gcctggatga gaaggtgggt
     8581 gttatcctgc ccaattttgg gcactgcatt cgctgaacca agaattgtca ccactgacat
     8641 gcccgaattt tcccttctgt caatcatctg ttggatttgg attaactgcc ggcttttttg
     8701 gcaggtgccg taccaataaa gtgatcgcag ccgccacggc gatgaccgcc attaaccact
     8761 ggttgctgtt gatcccggcg attggcgaat gcacaatgcg aataaactcc aataagaacc
     8821 tgccaaaggg gtagatgatt aaatacacca aaaaaacatc tccaggcttt aatttacttt
     8881 cgaattttcg cgcgatccac agcagcacca gcatgttgag gatgttccat atcgattcgt
     8941 ataaaaatgt cggatggtag cgttcaatat ttgcgtaccc cggcaggcgg tgagcttcat
     9001 cgatcttaat cgcccaaggg aggttagagg gcagcccgta gacctcctga ttgaaaaagt
     9061 tgccccacct gccaatcgcc tgccccaacg ccaacccggg cgctatcaca tcaagtaatc
     9121 gagaaaaggc gagtttcttt tttctgcagt aaaaccacag cgccagcaca ccagccatca
     9181 ccgcgccggg gatgcccaac cctccattgc gaatattgag catctcaatg ggtctcataa
     9241 agtatgggtt ctttccatct atgagcatgg tggaatcagg ggttaacaca tgccagatgc
     9301 gtgccccaat gattcctgca atcagcaccc acggcagcag gtcccaaatg atatccttgt
     9361 caagccctcg ccgccggagt tcccgcgagg acacaaccgc gcccagcaaa gccccggcaa
     9421 tgataattaa cgcgtagaag taaatcttta tgccaaaaat cgtgaaaccg tttatcattt
     9481 ttcctcttct atgtaccggt attgacttgc tcttttgatt gcttgtaaac aaaccacatt
     9541 cgctgaattg cagtgatgtt cgctccgatg gagatgatcc acaaagcgat gatcggtctt
     9601 tttataagca aaaaagcgac catgattatc atgcgctcca cccgggtaag cagccccacc
     9661 ttggctgtaa aacccagtga ttcagctcgt gctttggtat aagaaaccat tacactgcca
     9721 gccgccgcga taaacgtcag caggcaggcg ctcaagctct gctgctgaac atagtaaaaa
     9781 agcaatccta acaacagcac cagttcggaa tagcggtcgg tgacagaatc aatgaaacct
     9841 ccaaagcggg tgggttcacc cctcaaacgc gccatcgacc catccaccac atccaacggc
     9901 gctcccacca gcaaaatcaa acccgcgatc agaaacctgc ctgtggcggt aaagtatgcc
     9961 gccaggattg tgaggatgaa acccatcgct gtaatggtgt taggtttcaa tcccatcttt
    10021 atgaatagac tggcgattgg atcaatgacg cctttaaaca gcttccgcag gaaaatttct
    10081 aatgaccaag tgctttttgc tgacggctca gtatgctttt cttccattaa ttctttcatc
    10141 ctaagtgaga gattgtacta tagtctgaca tccgaggaat gacgaaaagt ctcacgcttc
    10201 atcttcttct gattctggct cctcttcttc aacttcaaga acctcccgct ctttaccgct
    10261 gccaactgcc gggccgatca cacccatctc ttccagttca tcgatcaagc gggctgcccg
    10321 agggaaacca atacgcagac ggcgctgtag tagagaagtt gaagccttgc ctgtttctcg
    10381 cacgatcgtt gtcgcccgct ccagcagcgc atcgaacccc tcattttctc tgttgtccac
    10441 cagtccctcc cacggtgggg caccatcttc ttcgtgcaca gcagattgcc aatattcaat
    10501 tatcttttcg atttcaccat cgctcacaat gaccccctgc agtcgctgca acccagaacg
    10561 agccgggtca agcagaagca tgtcgccttt ccccagcaat gtttccgcgc cattcgcatc
    10621 caaaataacc cgagaatcca ctgctgaagc catggcaaag gaaattcgcg cgggaaagtt
    10681 ggctttgatc agcccggtga ccacatctgt gctcgggcgc tgcgtagcaa ccaccaggtg
    10741 cataccggtg gcgcgtgcca tctgggccaa cctgatcagg ctgtgttcgg tttgttcggg
    10801 cgcgttaatc atcaaatcag ccaattcatc aataaagatg acgatcctgg gaagaggggt
    10861 cctttttttc tgcaacattt tctcgttgaa ggtatccaga ttgcgcgcgt gtacctccgc
    10921 cattcgacgg tagcgtgaat ccatctccat ttgcgcccag cgaagcaccg ccagcatgcg
    10981 atcgatttcg gtttccactt tccctatcaa atgcggcagc ccattaaagc gcaccagttc
    11041 gaccatcttg gggtccagca tcaccaggcg caggttatct ggtgtgttat tcatcgccag
    11101 gcaggcggct aacgcggtaa cacacactga tttccctgaa ttagttgtgc cagcaatgag
    11161 caaatgaggt aatttttcca gatcaacctg tatcggctcg cccgatacat ctctgccaag
    11221 cgccaataat aacggtgatt tcttttttct aaaggtctca gattccagta tcggccgcaa
    11281 tcgtacaaac aggttatctt cattcgggac ttcaatccct acgtaagatc ttcccggcac
    11341 aggggattca attctaagtc gttgggctga aagtgccagc ttcaggtcat cttggaggct
    11401 ggtgattttt gcaaccttca ccttctgcct ggttaccaca ccatccgcgc ctttgcgttc
    11461 agtgtagcca ggctcgacgg cgtattgtgt tacagcaggc cctaccctga atccaagcac
    11521 tttaactggt acgccaaact cagcaagttt ttcctcgatc aggcgcgcat tatctttgat
    11581 gagcacctgg tccggtggga tgatttggtc attcaagagc aggctcattg gagggagggc
    11641 agcgtcacgc cagctcacca gggaacttgg ttcggttttt tcaagttttt ccccggctgc
    11701 tttccgcgct gaaccaggtt tctcgctttt gctgattttc gtggcaggcg ctctgcctga
    11761 cgcctgctga tgcgctgtct ctcctctgct ccaggcgcgt gctttaccca gcacgcgcgg
    11821 aaagaagcca gtagagagcc cagcgaatac aaaaaataaa aacaaataaa ataacagggt
    11881 taatggtttt gaaatgagag ggatcagaag ctgtgccagc ccccacccaa aagtaccccc
    11941 atccagacca tcatatgcgc gctcgatcga tacgttgccg gtgaaagcca aaattggcag
    12001 aaggaccagt agcatacctt caattgccag tactttgcca atggataatg gaaaccgttt
    12061 gccggcagcg tgtctgaaga cactgacccc aacaaaaacc agcaccaaaa ccagcaggta
    12121 gctgcccaag ccaaatgccc ggcggatgag agtaatccag gaattgatga gggttccccg
    12181 gcttaaccct aacaaaccca gggaggtcag aatggcgagc agaatcagaa atgtgccaag
    12241 caggtcccaa ccaaactggc gaaagtgagt cagaatggcg tctattcgct caaccagagg
    12301 aacgaattct ttcggagcgg gattcttttt tggagggaat ttatccttcg ggctgggtct
    12361 tggtgtcatt cttcgctgat caatcccagt ccaagcctgc gccggtctgc atccacgtgc
    12421 aaaatcttca cttgcactct ttgacccgga gtgtaatgct cggcgatatt cattttgcgc
    12481 agggtctcgg ggaacgctga aatgtggatc agcccttcaa ccccttcatc taaccttgcg
    12541 aaaattccaa aacgcatgat tgatgtcagt tcagcggaaa ccacatcgcc tggtttatat
    12601 atttttgaca accgctccca tgggttaggc gtaagacgct tgtgactcaa tgcgatgcgt
    12661 gagttggctt cattaacttg caacacgatg actttaacct gttcgcctac cttaaaaaca
    12721 tgcgatggtt tttccacccg tccccaagac aactccgaca catggatcag cccttcaacc
    12781 ccgcccagat cgacaaaaac accaaagtcc gtgatattgg tcaccacacc gcaaacaatg
    12841 gtgccctctg taagttgggt gaacaattcc ttgcgctttc cctcccctgc ctgagcggcc
    12901 ctctcagaaa gaacaacacg ctcactctcc atatcgcatt caatgatttt tagatagaga
    12961 gatgtcccca cataattcgc gagtttttgc tgacggactt catcactgac ctctgcctga
    13021 aagtcaacca ggtgagagac cggcacaaac ccctgaatgc ctgcaccctg caccagcagc
    13081 ccgccccggt tatagccttg cacttcgagc agtaccactt catcatcagc ataaaccgct
    13141 ttgatgcagt cccaatcgag ttggctggtt gttttcgatt cacagggttt gcagtttacc
    13201 tctttatttt cgcgaaaaaa cttttcctcg tccgcgagaa ctgatgccca ccagccttca
    13261 tccatctctg gaatgctcgc ttcctgtttc ctttcctgta caatttccac cataaaagcc
    13321 cctccgaaca ataaaaaacg ataaacagag ccgtgcttat gtattcctgg tgttcagctc
    13381 catttgcacc agccgatccg cgattttttc gattgctact ttctgtttcc gatagaggtc
    13441 tgcttccgaa acggctaatt ttacagcaat ctcgcgtact ttctttccct ccaggaactt
    13501 catttctaaa atattataca ataaccattc gccggtgtat tttctttctc cttctggttt
    13561 gagctgttca agcgcgcttc gaaggacggt tctcaaagca ttgacttcgt tgttgtcgtg
    13621 ctttgccatg gcttctttta cgatttccag ctttagcaaa ggactatcag ttaattttgg
    13681 tcctccccaa taatgggtta atgccccttt tacccaggtg gtcatttcct tcttttccat
    13741 ccaatcggct tccatcagca taccgcgttg gtcaaatctg ctgactgccc gaagtttttg
    13801 aatcaattcc tcctgcggct ccaatctctc cagtgtgtca aaaacctgct ccagcatgtg
    13861 ccgatcgcgc aaggcgatcg cagcacgcgc cgccagccgc ttcagcgcag cctggtcttc
    13921 cgcagccaac gcggagatgt ctttgatccc cgtaatacca atgtatccca agaggtgttc
    13981 cccagaaccg ttactgttgt tgtgcaaagg aatcaggata tctgaccggc actcaacata
    14041 ttcttcatgg atatttcctg cgctttccag ataatccagc tcttccaata gttgcgggtc
    14101 aaatgaaaaa tcaggagttt tttcaaccag agtaaaacct tcatcatttt tctccagcag
    14161 ataggctcct tgcgcctgcg ctctatcgtt cacagccgca agcaccatct caataaactg
    14221 tttcaggtct ccgcgagtga taatacgctg ttccagtgtt agcacgaaac gcaaatcctg
    14281 ttcgtctcgg tcataaatca gccatctttc aaaagcgggg aaaaacaaag taatggcgta
    14341 ctcacatatc agtacagtca ctaccatcac tataggaaca aaagcgtcat acggggtacc
    14401 cagtgcaata ttcgctcgct tcaccagggt cattaaaccc agtgtcaaaa cagcggtgat
    14461 cggtcctctt aaagtccatt tgatcaagcg gctctttacc acgcgatctg gccaaggaac
    14521 cccaaagaac gccacagcgt atgccatgat aaagatacag atatccaacc cgatcgatac
    14581 tgtcgccgct aaaagccaga agagcaaagt ccattgactg gcgaactcgc tgctgtaaag
    14641 caagaaaggg aaagccccca caactggacc aatcgcgctg attgccaggt acaacatgcg
    14701 ccgtttactg gtgcgtgtga gcgtgcgctg gtaggctcgc cgaaaattaa cccaggcgag
    14761 caccaaaata atgatgtaat aaacaatgaa cagctgggta tatattgtgg gcaagtgatg
    14821 aggagcaggc ggttgatcaa gcaccaactc acccagaaac cagggcgtaa acaggaaaaa
    14881 gatcaggatg aatgaaaaaa ggtaagttgc cctgatcgcc cacctgcggc gccagcgaga
    14941 ggggcgcccg gtagtggcca acagcgcatc agaaaaattc aggtaggcgg caggcagcag
    15001 gatgattccc acccactgcg cgcgcaacca gaagtcgatc atgtacgggt ctttgcttgt
    15061 cgccccgaat gattctgtag aaaacacgat gaccaggcag accataattg tcgcgaacgt
    15121 tctggctact ctttccagca gattgaacgt caggctgtat aacagcagcg caaaagcagt
    15181 aatggcaatg ccggcggtta agatttcact tattgtcctg gctatcgcga tgatttcatt
    15241 cacccagtta ctgaccatca tcttcctact ttaattcact caaataaaac aacaccatgt
    15301 ccccattttg gtagaaattg tccacctgac cggcgtgaga gaagcgtaat tttacggcca
    15361 tctcaaccat ggcatggttt tttgccggta tttcgagcaa catccttgtc atccccctct
    15421 tcttcaccca gtctctcgcg gaaaaaacca gcgcgctggc gataccctgg cggcggaatg
    15481 gggtattgac aaccaggtcg tgtacaaccg ccaacaattt atcgttatct tcatccaacg
    15541 tcaggtaccc tgccaggctg ccttcacaac tggcaattaa gaaaagcaaa tgccgggtcc
    15601 atgtgtttct gagcagttct gccctgcgcg gatagttcaa tcgcaatacc cggggaagtt
    15661 ttacctcatt gagttcaatt gcaatccggt cttttgtggt gctcccatcc actcgccata
    15721 catgcgtggt ttcaatcgta tggtcaaatt tctccaatgt tgaaatatcg cttgataccg
    15781 cggggcgaat ttcgacttct gccattttta ctccgatgct tttatggtca ccaggatcac
    15841 acatgctggt agcgaattat tttggatgtc gttcaccaca aggcgcagtt tgtattcacc
    15901 tggaattaat gcccgggtat cccacgttcc cccaaaaggt tcattaatga tttttgcatt
    15961 tcccgcagcg atggttgtcc atgtatcact atctgaaggc gaatactcat atttataaaa
    16021 gcccaggttt tcaatattta ctgtgccttt cagttcgacc ctccccgaga gggtatctcc
    16081 gtttttcggg tctgtccaat caatctggtc aggaatacat ccacggctaa agtaagaggt
    16141 aatggtgggg atcagccctt ccccagtaac catcaatatt ggcgctgggg tttcagttgg
    16201 cggtagcgcg gtcaggtcaa tggtgggtgt gggcaattgt tgcactcccg gtacagcagg
    16261 agtgataaaa gtattgacca ggaacaatcc ggttgccagt aatacgctga aaaccagtac
    16321 agttaaagcc gagttaaata tctgttgagc atgttctttt tccagcccga aaatactcct
    16381 ggaccactcc cgccacgcca cgtatgtttt cctcgcggac gcgaggatga tcaggccggc
    16441 aaccaggaaa atgaggatct catatttttc gaggaaacgt agaatactgc tcacgataat
    16501 ttgaccaata aagatgcatt caatgctaga acctattcgc ctgccagggt ttcaatcaca
    16561 ccgcgaatga tcgtggtcag tttgggtacg atcacttttc cggcttccag gacctcttcg
    16621 tgagtggtga ccgtgctgcc atccaggttg gctttattac tgattcccga taaacccagg
    16681 acacgcatct ttgcgtgtcg tgccacaatg ttttctggta ctgttgacat cccaaccgcg
    16741 tccgccccga tgatcttgag aaaccgcagg tccgccggcg attcaaacga agggccagcc
    16801 agacaaacat aaaccccttc atgcagctta atgtgatgtt tctgcgctac ctgcctggcg
    16861 gcagcgataa agagggagtc ataaggctgg ctcatatccg gaaaacgttc gccaaattcg
    16921 ttcaaattgg gtccgcgcag agggttttgc cccgccatac cgatgaggtt aagatggtca
    16981 gtcagcagca tcacatcgcc cggtacataa tccgggttaa tggcgccagc cgcgttggtt
    17041 agaaccaggt tgcgcacgcc catgcgctgc atcacccgta tcgggaaggt gaccctgtcc
    17101 atcgagtacc cttcataata atgcaccctg ccctgcatca ccatggccgg caccccgaaa
    17161 agccgcccga aaaccaggcg cccagaatga ccctgaatgg tggataccgg ccattcaggg
    17221 atctggttaa agggaatgat tctggcgtca ttcaccgcct ctgccaagcc ccccaacccg
    17281 gatcccagta ccagcgcaac cacaggcttt tcaacgattt gcctggttat aaaattcgca
    17341 gcactatcaa tttctcttat cgtgataaaa gaatccatgc tcacctcaat gcttgcctgc
    17401 aaagtggaat acctgttttt attatatcag aatgacctta agcgattgtc agctgtttac
    17461 gcgccagaaa gcgcgctgcg gaatgcccgg attgtttcgt caacgtcttt atctgtgatc
    17521 caataatgcg tcaccaggcg aaacctgcgc tgagcaacca cacccacttt gacgcccagc
    17581 cttgccaagc gctcggcaac ctcctgagca gaaaaaatga cctcttcctt gagcgaaaga
    17641 aaaaccatat tactttgcgg catgcctatt tcaaagtgaa tgccaggcac tgtcatcaag
    17701 ccttctgcca gcagagttgc cctttggtga tcctctctta atctggtgat catattttcg
    17761 agggcaaaaa tgccggcagc ggcgataatt cctgcctgcc gcattccccc acctaacatt
    17821 ttacgcacgc gcaacccttt cttaatgaac tccttcgtcc cacagaccac gctgcccact
    17881 ggtgcgcaca gccccttgct caggcaaaag gtcacgctat ccactggctc cgtgagagcc
    17941 ttgacatcaa cattcaacgc tgctgcggca ttgaagatgc gcgcgccatc aaggtgtaaa
    18001 tggagatgat tgcggtgcgc caattctgcc acttcacggg tatacctctc agtgagcact
    18061 gttcctccgc atcgattgtg cgtattttcc agtatcacca tgcgcgtaat ggggtcatga
    18121 atattatccg ggcgtatggc gttttcaata tcttcaagca tcaggctgcc atctgcctgg
    18181 ttgggcaggg tatgcgcgaa tatgccgcca agagcggaaa tcccgcccgc ttcataaaga
    18241 aaggtgtgcc caaggttgcc cataatgacc tcatctcccc ggttgcacac gcttaaaaca
    18301 gaaagaaggt tccccattgt tccactgggc acgaacaacc cggcttcttt gcccaataaa
    18361 gcagccgaca tttcctgtaa ccggttaacg gtgggatctt ctccatatac atcatcaccc
    18421 acttcagtag ccgccatcgc ctggcgcatt tcttcagtag gagtggtgac cgtatccgag
    18481 cgtaaatcga tcaatttcat caggctctcc gcgttagata agttgatcca ggatggtttg
    18541 caaatcagcg ggtaattccg cttgaaaggt tcgggcttcg ctttcacccg gtaaaatgat
    18601 gcttatctgg taggcgtgca agaaatgccg ctccaaatca agcgagggct tttttctgcc
    18661 gtagacaagg tcacccacaa tcgggcagcc cagaaacgcc atatgcaatc tgatttggtg
    18721 cgtacgcccc gtcagggggt gtgcttctac caaggtgtgt tggttgaatt gcttgacggt
    18781 gtgaaactcc gttatggctg ctcgtccccg accatcagaa accaccgtca tttgtttgcg
    18841 atgagccgca tcccgggcga ttgccgcttc cacccggcct gatggcgttg ggggttttcc
    18901 atccaccaat gccaggtaag tttttttgac cttgcgtaaa cggaactgtt cttggagcca
    18961 gcggtgggcc tttccattct tggctacaat gatcaacccc gaagtgtcct tatccaggcg
    19021 gtgcacgatt cccggccgta cttcgccgcc aatgccctgt aaatctccaa aatttcctaa
    19081 cgccgcgtgc accagtgtcc cgctggcatg gcctggggct ggatgaacga ccatcccggc
    19141 aggtttgtta atgatgacca gatgctcgtt ttcgaagact atatcaagcg ggatactctc
    19201 cggttcaagg ttaaccggta ccacctctgg aatgcatatg ctgactttat caccagtcag
    19261 cagcgccagc cctgattttg ttgccacggt ttcattaacc gtcacaccgc catccttaat
    19321 aatcgcctgc aaccgggaac gcgactgacc cggtaattgc cgggccagga atttatccaa
    19381 cctgttctca tctccgcttt gcacgataaa ctggcagcgc tgtgagctca agggctaacc
    19441 tcattgtccg cctgctcctg cgtggttttt gctttcccgt ttttctctcg tttttcttgc
    19501 agccacacac ccagcagcag gatgataacc cccacagtaa tgcacgcgtc agcgatgttc
    19561 cagaccggga aagttccaac tgagatgaag tcaatcacat gaccatactt gatcctatcg
    19621 acaaggttgc caaccgcgcc gccgagttcc atcatcaacg ccagtttgat catccagtct
    19681 tttttttcta tcttagggta ataatagaca ataccgaatg aaaccagaac agccagtcca
    19741 ataaacaccg ggttcatgcc ctgaagcatt ccaaaagcca caccggtatt gctccaatgc
    19801 acaatccgag catagggtgt caaccactcc catggagacc acacctcgcc ataggggagg
    19861 tgggtaatta caattttctt caatacctgg tccagcgtaa caatactggc agccacaaca
    19921 aataaccaga cggttttctt taaaaagtgc ttcaattaag gctccatttg acagtggttg
    19981 aattttaccc cagaatgcag atgaaaagcc tggggctcct ccacggagcc ccagaagttc
    20041 aggctttgat gatatgtatg gttacttttt cctgatcaaa ctcatctgtg accgtaaacg
    20101 ttccctctgg cgctcccccg ccctgaaaag cgactgcgag ggtctccatc ataatgtaat
    20161 cacgataagt ttcaaccgcc tgcgccagga gaggtgtggc gctataataa agccggatac
    20221 ggtcagcgat atccagatct gctgttttac gcaagtcctg tacccggcgc acaaattctc
    20281 gcgccaaccc ttcgctgatc aagtcggggg tcaattcagt cactaacgcc gccagataag
    20341 ccccttctga cgccactgag tatccttctt tcgcctgcag tctaacctcc acctcttcag
    20401 ggagaatatc caaaacattc ccgcctgctt ctatgcgcaa actctctcct gccagcagct
    20461 tcagtgcgct ggcttccaca tccagcgcca aaaccttctt gcggatttca ggatacaggc
    20521 tgccgtattt ttgtcctaac tgcttgggca acgggtttaa tgtataactc accgcctctc
    20581 ccgcggcatt caacgtcctc acctttttta cattcaactc atccgccaat aattcagcat
    20641 tacgctttat tacccgctcc tcttcctggc tgcccacaga aaaagccact tctgcgaggg
    20701 gctgcctcac cttacggttg gctttgttgc gcgcagcgtg ccccaaagag gtcagcttca
    20761 taaccagttc catctcgtca ttcaatttct cgtcaatcgt ctcgctgtca aattcaggcc
    20821 acggcgcaag gtgtaccgat tcccatgcct ttttatccac cgaacccacc aggttcaagt
    20881 acaactcatc cgcgataaaa ggcatggttg gcgcaagcaa cttgcttaga gcggtcaggc
    20941 attcataaag agtactgtaa gctgcgttct tatccgcatc actttcgctc ttccaaaaac
    21001 gtctgcgtga gcggcgcaga taccagttag agagttgctc cacaaatgtc tgcaccggcc
    21061 gggttgcgcc cagtacatca taattttcaa gcgctatggt gacgtctctg atcaacgcgt
    21121 gcagcctgga gcgcatccac ttatccaggg cgctgtaagc caccttttcg tctggtacgg
    21181 ggctccattt atccagattg gcataagtca cgaagaaaga ataggtgttc cacaaggtca
    21241 atgtgaaatt acgcagtact tcacctacca ggtctgcgga gaaacgccgc tcctgtcctg
    21301 gcgggctggc ggtataaagg taccagcgca tcgcgtcagc gccatgcttt tcaataaccg
    21361 accagggagc gatgacattc ctgcgggatt ttgacatttt ctgcccttcg ccgtctaaga
    21421 tcaaacccag gcagatcaca ttcttaaaac acttctcatc aaataacaaa gtgctgatgg
    21481 cgtgcagcga gtagaaccaa ccacgtgttt ggtcgaccgc ctcgcaaata aaatccgctg
    21541 ggaactggct cttaaacgct tcgacgttct cgaacgggta atgccactgg ctcacaggca
    21601 ttgagcctga atcaaaccaa acatcgatca actcggggac gcgccgcatg ttcccgccgc
    21661 atgcagaaca cgcaaattgg attttgtcta cttcgggtct atgcaggtta gcgttcgcga
    21721 ggtttctgcc ggcacgatct gatagctcag caattgagcc tatgcattcc tgttgatggc
    21781 aggtgtcgca ctgccaaacc ggcagcggtg tgccccaata gcgttcccgc cccaaagccc
    21841 aatcaacgtt gttttcgagc cagttgccaa accgtccttt acggatgtgt tcaggatacc
    21901 agttaatttc ttcgtttaac ttcaccattc gttctttata ctggctggtt cggatatacc
    21961 aggttgggcg cgcgtagtat agcagcggcg tgtcgcatcg ccagcagaag gggtaagtgt
    22021 gcaaataagt ctcttctcgg aacagcaatc ccctgttttt caaattttgg gtgatttgcg
    22081 ggtcagcgtc cttgacgaaa atgccggccc agggatttac ctgcgggatg aatgtgccgt
    22141 cttgagcgac cgtgttcaaa atgggcaggt catgctcgag cgccacctgc atatcatccg
    22201 cgccaaacgc aggggcaatg tgtaccaaac cggttccgtc ttctgttgtc acaaactcac
    22261 ccagcaccac gtaatgggcc tttttttccg cgggcaggaa tgtgaacaat ggctggtagc
    22321 gcagcccctg taaacgtttc cctttaaaac ggttcatcac ctgcactgtt tcaccagcaa
    22381 atacgcgttc caccagtgat tcagccagga tcaactcctc tttggatcca tcctccattt
    22441 gccgctcaac agtcacgtaa tcaatttcag cgcctgccgc caccgctacg ttcgccggta
    22501 aggtccaggg agtggtcgtc cacaccagca gggaggtacc cggggtgtca accagcggca
    22561 tacgcacgaa caccgatggg tcaggggcat cacgatagcc ctgcgccact tcatggtctg
    22621 aaagcggcgt accacaacgc gggcagtacg gcaccacctt atacccctga taaaggaggt
    22681 tcttctccca gaagctcttc agaatccacc acaccgattc aatgtattca ttggtgtagg
    22741 taatgtaagc gttggtgagg tccacccaat agccaatgcg gtctgtgagt ttttcccact
    22801 cctggatata agtgaaggct gactcgcggc aaaggtcatt aaacttgcca atcccgtagt
    22861 cttcaatttg ttgtttattg gtgaagccta attttttctc cacctcaatt tcgactggta
    22921 gtccgtgggt gtcccatccg ccgcgtcgtg acacgtggta tccgcgcatg gtcttgtaac
    22981 gcggaaagat atccttaaag gcccgcgcca gtacgtgatg aacccctggt ttgccatttg
    23041 cggttggggg tccttcgtaa aatacatatt caggaccacc ctcgcgggaa ctcatgcttt
    23101 tatgaaatat ctcgtgtgtt tgccaatagc gtaatacttt caattccatg tcagtgatgt
    23161 ccagctttgg cgatacaggt ttaaacattc ttcctccgat aacaaaaatc ccatccctct
    23221 ccagggacgg gaaaatgggc ccgcggtacc accctgcttc tctttcttga gcgctttgcc
    23281 aggtgcattc tgtcaataga cctgatccac tgtaacggtc ggatttccgg ctcacttact
    23341 tgcacaaggc tttcaggtcg cagctccgga aggattttca gctcattcac cagcccggct
    23401 ttcacctcac ccggtctcgc tgttggttaa cgcgagctta ctcgtttcca tcgatgcttt
    23461 tattagcgga attatctcat aaacatttca aaatatcaat cttttatttt tgcttgatca
    23521 cttcgtaaaa taatggcagc ggctcgcagg gctgctcctg ccaacggcag gcggagagca
    23581 cgctgttggc tgccatttgc catgagcttt catccctggc gtgaatggtg aacaactcgt
    23641 cacccgtatg caccaggtca cctaccttgt gatgcacaat gatgccgacc cggtggtcaa
    23701 tcgtatcttc tttcttattc cgtcctgcgc ccatgttcac agaagcctcg ccgatctcca
    23761 gcgcgtccac acggctcagg taaccctttt gtggagactg caccccgcgg atgatcggtg
    23821 cgggagggaa taaagcgcag tcatccacat atgcggcgtc gcctccctgg ttcttcacca
    23881 gttgtttcag tttttcgaac ccgctcccat ctgccagcgc cgcttctgcc attttcctcg
    23941 ccgcatccag gttttgcgct ttttcaccaa tcagcagcat gtgtgaagcc accaccaggc
    24001 aatgctcagt aaaatcggct gttccccggc ctttgagggt gtctatggct tcaactaatt
    24061 ccaaggcgtt gcctaccgcc cgacccagcg gctggttcat atccgaaagc aacgctacca
    24121 ccttgcgccc gctcaatgta ccgatggtca ccattaaacg agaaagctgc ctggcttctt
    24181 ccaggttttt cataaacgcc ccactgccca ctttcacatc cagcacaatg ccttgagcac
    24241 caccagcgat tttcttactc ataacggatg aagcgatgag aggaattgat tgcacagtgc
    24301 ctgtgacatc gcgcaatgca taaagtttgc catcggccgg cgccaggtct ccgctttgcc
    24361 cagccaatac cagcccttca tcgcgcagta agcgtttgaa ttcctcctgc gtgaggttcg
    24421 tgcgcatgcc tggaattgac tcaattttat ccagcgttcc acccgtgaat cccagccccc
    24481 ggcctgacat tttgccaaca ggcagcccgc aggcagaaac aagcggctca accaccaggg
    24541 tggtcttgtc tcccactccg ccggttgagt gtttatcaac ctccatgggt acgattcctg
    24601 aaaggtcaag cacctcacct gaatacgcca ttgccagtgt caggtccgtg gtctcctggt
    24661 cgctcatccc attaagcagt accgccatcg cccaggcaga cacctggtaa tctgggattt
    24721 cgccgttaac gattccgcgc acaaaaaact caatctcttc acgggtcatt tccttgcggt
    24781 cacgtttctt gataataata tccactgctc tcatgggcgc tccgtctcgt tgatgattgg
    24841 atgattattt tactatagcc atcatcgaaa aacgccaggt cagttgacct ggcgttcatc
    24901 aatcgatatt cacggattaa gggttcctga gcgcgatcgt caccttacgg taaggttctt
    24961 ctcctacgct ttcagtgaaa acctgggggt gatcgcgcag ttcaaggtgt atgatgcgcc
    25021 tttcatttgc aggcatcggt tcaagggtct gtttgcgccc agtctgtacc gcctggtcag
    25081 ccatgcgtct gccgagctgg cgtaattgcc gttctcttcg gctacggtac ccctggatgt
    25141 cgatcagcag cggtacccaa cgccccagtt ctttgctgac gatcagcccg gtaatatatt
    25201 gcagcgcgtt aagcgtttca gaccttctac caattaaaat actgaggtct tttccctcaa
    25261 catctaccaa caccaccctt tggtcttttt catcttgtgg ttcgatatac ctggcggaga
    25321 catccgcttt cacccgcatt cgctcaagaa gctcaatcac aactgatctc accgatttga
    25381 cattctgctg gtcttcttcg ctcaggtcag ccaggtctat ggcgtgtacc gaggagggct
    25441 ctatcgtgat ttcttcacta tcagcgtccc cggtgaaaat tggctcgtgt tcatcgatca
    25501 acgtttcaaa tgatttcaca gttaggcgca ccctggattg cctgccgcct aatcccagca
    25561 gcccgcggga accgccgtcc agaacctcga tttctaccat gtcttctgtc agacccaatt
    25621 gctcgagacc tctctccacc gcctcttcag tcgttggcgc gataatttcg agcgttgtct
    25681 tttcagtgct catctgtcac ctcagtatag ttgattcagc gttacttttt tccttctggt
    25741 ttctttttcc agaaaaccac attatcccaa tgtactttgc cagaaatcgc gtactgggcg
    25801 atttggacga agttcgtcac tacaaagtaa agcgccaggc cagattgtaa ggaccacgcc
    25861 agccagccca tcaagaccgg catgtacaga ttcatcatct tggacatcat cgccccctgg
    25921 tcagcagcgc caggctgctg gggaggggtc atgattttgg ttgagatgta ggttgaaatg
    25981 accacaacaa tcgccagcac agggatacca aagttcaatc ccggaatgtg cagacgctca
    26041 ggcattccaa tatccatcca taagaatttg ctgttgagtg gaagcacctc tgcaatgcta
    26101 aggaatttcg gataaatatg tcttgtcagg ttgagcagat ccagcggacc agtggccatg
    26161 gtttggatca tcgcctggta cagcccgatg ataatgggga attggatcaa tgtaggcagg
    26221 caagacgcca tcgggttgat cttcatttcc tggtacagtt tcatctgttc ctgagcgagc
    26281 ttttccttat cacccttgta cttggtttga atctcgatgt accgtttatc cttctgaagt
    26341 tcctgcatcg cggcggagct tttaatttgc ttggccgtca aagggtacaa cacaatcctg
    26401 gtcagaatgg tgaagaggat caaggcaagg ccaaagttac ctaccagctt ggagatccac
    26461 aacaacaagt taataaatgg ttcaataatg atggtatccc acatggttgt ccgtccttat
    26521 ttctttttgc cgtaatacca gttttcatta ccgttggtat caagagcgat caacggtttt
    26581 tcgcttttat tcaggctgat aaccaggcgg tttcccagcc gttccgggct tgcgtagatg
    26641 ctgccatcaa atgagcgagt ccataaacgt tcaccattga aacctacaag gatgacctgg
    26701 ccatcttcat tcccaaacac aaggccctca tccagcagcg ctccagtgcc cacaattgcc
    26761 gcgtcggttt caatttgctg cacgcttttc ccattctcgc tattcaaaat attaatccgc
    26821 ccggattggt ctccaaaata cagcttgccg tcatggagaa ctggctgcga ccacactccg
    26881 ccgcccactt tctgctgcca gatgcgcgta ccgtcttccg cgttgaacgc gaaaaaggtg
    26941 ccatccagat tgcccaggta gagaacgcca ttctccaaca acgggcggga gatagtggtg
    27001 gtatccaggg ctaccttcca ctttaaagcg ccggttgcgg catccaacgc gtaaaggtat
    27061 ttatccatcg agggaaaata aaccgtggtt ccatcggttg cgggtctggc ccatagagca
    27121 tgcccggcct taaactccca aacgaggttg ccagaaagat ccagcgcgta aagagaataa
    27181 tcagtattcg gcgcgagaat caagttatta acgaccagag ggctgtcaat ataacgtccc
    27241 tctgcctgtg taaatttcca ggtttctgca ccagtcttcg cgtttagaca ggtcaactgg
    27301 tgcgcataat ccaccacgat caactgctca ccaatgagca caggttcggc atagaaaagc
    27361 cgttgggggc tacccttttc tggatattgc caggcgacat ttccactatc ggcaaccaac
    27421 gcggtcacct ggcttccgcc ggcataatac acgtagtctt cggtcaccaa cacaccagac
    27481 cagctgttag actgaccggc gcgtgagcat gccgtgagtg ttactgccag cagcacaaat
    27541 agcaggatca aaccaattct tcgaattttc atgctttttt cgttccctat tattaagaag
    27601 atatccttag accgaggtca cgggaccggg tcaaatccac ctggattaaa aggattacaa
    27661 cgtaaaacac gccagacagc caggatcgta cctttaatgg cgccatgttt ataaatcgcc
    27721 tgatagccgt aatgcgaaca ggaaggataa aaacggcagg tattggcagg caacgctgga
    27781 gaaaccagcc attgatacag cctgatcaac cctaacagag gcatgcgggg tatattaacc
    27841 aatcgaaatg gaagatcgcg cagacgtggc tcagccacat gaacgtgttc atcttcctgg
    27901 gcgataattg tttgagccaa tcaatcctgc ttttcccagc agttcaacaa ctgctgcttg
    27961 aatttcctga aattgcactt gtgaggttct ttcacgcgcg atgagtagaa agtcaatagg
    28021 gtctttgagt tcagagagat atggtgcaat gatagcccgt aactgccgct tcacccggtt
    28081 gcgctcaacc gctccgccta ctgaccgtgt cgcgatgatg cctgcccgtg aattctctgc
    28141 caatcctttt gcacaaacga ggactgcaag cgggtgcgca tacgatcgac ctgtgtggcg
    28201 cacccgcttg aagtcatttg tctgggttaa tcggaacttc cgtttcaccc gatgtccagt
    28261 ccgaaatcct gaaaagcatc ttgtggatac accgttcccg gccgttcaga aaaatcacca
    28321 gctgatcttc ttgacatggt tgttcattgt aaccgtcagg tgatgacggc ccttcgcgcg
    28381 ccttcttttt aatgtcgcgc gtccatccgc tgtagccatg cgcgcgcgaa agccatgcac
    28441 acgaacacgg cgtcggattt tcggttggta agtcctcttg gtcatgccac ttcctctcaa
    28501 ttcattacag aatgcttaaa ttcgcaaact tggattatac ctcaacctcg ttattcggtc
    28561 aaaggttttt cacgcgcggc gcatcgagca gttccgccac catcctggca ccttctgcgt
    28621 cggttaagac cagcaattcg tcctcagcag ccagagtggt cactccccgt ggaatcacaa
    28681 gctcaccatc ccggataatt cccgcgatga cacagttggc gggtaatttc aagtccttga
    28741 tcatcatgcc taccgcggtt gcattgacag ggattttttc ttctaccagc gagtattttc
    28801 ctcggcgcag cttgagcaat aacatcatat cgcccattga catttcttct tcgatcaggt
    28861 gagccaaaat ctccgcctgg ttcaccgcca catccacatt gaacatcttg ttgaacaacc
    28921 atgcgttacg cgggttattc accctggcaa tggttcggtt cacgtcgtat ctttctcttg
    28981 ccacgtaaca tagagcgagg ttttcggcat ccacaggagt ggtggctgca aaaacatccg
    29041 cgtggatcag gtccgctaac tccatcgtgt gggtgtccaa atggttgcca gcgatgatgg
    29101 attcagtggg gatttcttta tgaatgcgcg caaggatctc ttttcgcgat tcgatcaaat
    29161 gcactcgatg ccccgcgctg accaacgatt tcgctaattg cgctccagtt cgtccgccgc
    29221 ctgcaatcac aacaaacata ttaactcacc tgcctttttc tgaggttctc acgcgtttct
    29281 gtaattcctt caaacgtggc tgccacggtt aatatatccc ccgccttaat gaccgtatcc
    29341 tggtctggta agaatgcgct gcccgcccta gtaaccgcca gccatttgca tttgctgcag
    29401 gttaccagat cagagacttt tttctcattc cagctcggcg gaatgataat ttcgtaaatc
    29461 tcaacctcgc cgttgcccgc cgaaaaaacc gttcgccctt ctgcgtcaag tagaagctct
    29521 tcaatacgct gggctcccca acttgaggag ctgataacct ggatattgaa tgtttcgaaa
    29581 atggaacgca aatgcgtatc ataattcctg gcaacaacgt tttttacatt aaattcgttt
    29641 ttcgccacat ggctcaccac cacgttaagc gcatcattat tcgtgacagc tgccagcgca
    29701 tcgcaggttt gaattcccgc gcgcgtcaga agatcctgat tgatcgcgtc accttcaaga
    29761 gtctttccct caaaatcaga agggaggttc ttaaaggaag ccgcgttttc atcgataact
    29821 gaaacctggt gtcccctttt aaaaagattg taagccagat cagccccaat ccttccgcac
    29881 ccaatgacaa tgattttcat aattttctaa ccctttcaat caaccaggta tggcacttcc
    29941 atgatcacaa tatccttacg tgataataac accttacgca gtaaatccgc agtgcgcgaa
    30001 tgtaaaaggc tggcaattcc acgccggggg atgaactgcg gcacgatgat gctgatgatc
    30061 tcgttcggcg gtcgattctt gtcgatttcc tcaatgtatt ccaacaatgg ttcaatgaac
    30121 aatctgaatg gcgactcaat tacaaccatg cggtagcctt ccccccacat ctcccacttt
    30181 tccagcatgc gcctggtttc cagcggatcg atggacacat gaacagcggt cacatcatca
    30241 gaaagcgtct tggcgtagcg caacgctgtg agcgtccctt tatgcactcc gctcaccagc
    30301 aacagcacgc gttggtgtac aatgcgccct ggtggggcgt attcttccag cgaaaggtct
    30361 tttgccaggg tgtaataatg cttatggatg gtggaaaaac ccaatactaa cgccgggatg
    30421 aggaaaacaa cgatataagc gccatctaca aacttggtga cagcaaaaac gatcatgacc
    30481 acgaatgtga tgagcgcgcc gaacccattg atgagcattt tgtgtttcca gccgctgtca
    30541 tacttgagca gcgaaccacg ttcctgcact tccacgccag gttcgagctt gcctgatttc
    30601 caccagcgcc gcgccatact gctttgcgat atagtaaatg agaggaacac gccgatcgcg
    30661 tagagtggaa tcagccttga aacgctcgcc tgaaatatga tgatcagcaa agaagccaca
    30721 atggccagcg cggcaattcc tcttgaatac accaggcggc tgccgcggta ggtcaattgc
    30781 ctgggtaaat atccgtctgc tgccgcaatg gcgcttaaac gcggaaaatc agcaaaggca
    30841 gtgttggctg ccatcaccaa aataatggtg gtagcagcga tagtgccaag gtagagcagc
    30901 cctctgcctt caaagatggt tcgagacatt tgtgagatga cggtttcttc ttcagaaggc
    30961 atcaccccca cgtggttccc caaaaaagtg atagaaaaca tcatcgatcc caaaatcagc
    31021 gccatgatga tcagcgtggt gcctgcgttc ttgctgcgcg gttctttaaa cgccgtgatg
    31081 ccatctgaaa tcgcctctac accagtcacc gctgaggttc cgctggcgaa agccttaagg
    31141 acaagaaaga gcgtcaccgg ctgcaaggca gattcaggca aaatcagatc cattgggggg
    31201 gcttccacgc ttcccagcgt gccggcaatc aggcggatga acccgacaat tacgttgata
    31261 tacatcatcc cgaggaaaaa gtacgtggga atcgcgaagg taattccaga ttccttcacc
    31321 ccccgcaggt tcaccagcat gatcaagagc acaaaactga ccgctatcag caccttatag
    31381 ggctcgaaca acgggaaggc agagattaac tgcgccacac cggatgagat agacacagaa
    31441 accgtgagga tataatcagt tagcagcgcc gccgccgcga tttgcgcagg tattgcgccg
    31501 atattatccc gcgccacgat gaaggagcct ccgccgtttg gataggcgtg gaccgtctgt
    31561 tcgtatgaaa agatgaggat ggccaggagc gcgcaaattc caatcgcgat cggtactgaa
    31621 aggtgaaagg cggtcacccc cgccgctgcc agaatgatca gtatttcctg cggggcgtag
    31681 gcgaccgagg acatcgcatc ggaagagaat acggccaaac ccaagacttt cccgatggtt
    31741 tggtgcgggg catcagcggt agaaagtggt ctaccgatga ggaatcggct ccagctcctg
    31801 cctggttgat attcagttgc ccggcgaaaa acggtgga
//
LOCUS       DAOTLW010000022        31605 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1699_length_31605_cov_14.662758, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000022 DAOTLW010000000
VERSION     DAOTLW010000022.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 31605)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 31605)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31605
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1699_length_31605_cov_14.662758"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..896)
                     /locus_tag="P5516_05035"
     CDS             complement(<1..896)
                     /locus_tag="P5516_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91837.1"
                     /translation="MRIKILLAVVLTLSLILSGCAEQPVSEQLVQEKLAVILTQSALQ
                     EPLEQPTQPALPEPPEQTAVTLPTHTPAPAPTSDGQQPPAPTPDASIVLTDIRETAPG
                     QAIVKWEAYGNFPSGYALVWTSEQRAPIYPKDEDAYVGDPNARSALISGEQGKVYIVS
                     VCRYVSDSCDIYSNLGFFAFKRYAPTPTPNRTATARALTPISVGGGGGGGSVGKETPS
                     SAFTITYMGGGATGKTQMTWKSDSNPSGGFRIYYSTSNQEPAFGKDSYFSISDGKVRE
                     AYVPGSSGTTYYYRLCKFNGSS"
     gene            complement(1064..1438)
                     /locus_tag="P5516_05040"
     CDS             complement(1064..1438)
                     /locus_tag="P5516_05040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91838.1"
                     /translation="MKRSAPLFKYERHNQPLLPKRQFLFRLLRHFGFGTGLILGSLAI
                     GVLGYHFTENMPWLDSVLNASMILGGMGPVNTLATAAGKVFASLYALFSGVIFLVAAG
                     VFIAPVVHRWLHKMHLDQDEEA"
     gene            complement(1535..2011)
                     /locus_tag="P5516_05045"
     CDS             complement(1535..2011)
                     /locus_tag="P5516_05045"
                     /inference="COORDINATES: protein motif:HMM:NF017262.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HRT91839.1"
                     /translation="MDGRLLVAVLVRFLHDLFTVVWMGGLIVTVITYLPLLQKTFAPG
                     AQLKQFMRAFQRQQGRWVHVCIVGLIVTGVLMSRHAAGPGQFFTFNNSYSAWLSVKHI
                     LVIVMIAIAFYRSIALGRKMESLSSAQMKLSMLLIILNAVLAAAVLFTSAMLSVLV"
     gene            2100..>2576
                     /locus_tag="P5516_05050"
     CDS             2100..>2576
                     /locus_tag="P5516_05050"
                     /inference="COORDINATES: protein motif:HMM:NF012655.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRT91840.1"
                     /translation="MQDYGFSGRIRQMNDRSQNQGKANPVRSRAQIIGASHRLFLMQG
                     FHGTTMRQIAKESGMALGSLYNHFTDKEALFSAVFEAYNPYPTLLLTLENSQGENAAA
                     LARTAARRLVNALSARPDLVKLVFIDVVEFHGEHLRQAYPAVAPGMLNFVRRLRKDP"
     assembly_gap    2577..2676
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            2820..4079
                     /locus_tag="P5516_05055"
     CDS             2820..4079
                     /locus_tag="P5516_05055"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRT91841.1"
                     /translation="MKKTELKLKNDVALIHRGKPTKRVLEVELTAPESSGARDATALN
                     LALIIDRSGSMAGGKLEQVKAAVLQILDLLRPVDSVAIVDYDHQVSITAESGSVTPSA
                     REEMKQAVSSLHPRGMTDLGGGWLCGCERLAHHQSNGRITRALLLTDGLANQGITHPL
                     ELQQHASALFERGIATSTFGVGEGFNEHLLEGMANRGGGNFYYIESREQIPELLLQEF
                     KDLVAVSLKRVNLELTFPAGVAFELFGDWRVEKSDDRHLVINLSDLSSGRAVNLFLSL
                     LTPPGDGELVIHASITGMDEADKPVHIEGALPLRYADAAEVALAEEGRDQDMLKRYSS
                     VVIGQGASQALKLEREGKFAEAGKLMDQLMLEHQDYLPTMTRERYQLIREEIRVGLDE
                     PRRKRYQNDSYILKRHRHIDQQGEPGK"
     gene            complement(4725..5123)
                     /locus_tag="P5516_05060"
     CDS             complement(4725..5123)
                     /locus_tag="P5516_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91842.1"
                     /translation="MPEDKKQRRSALDPAVADLLKGMQQKQSEAQLPRRERERISRER
                     AKIQARRDQRATYDLPPSIREGLRLLAEELRLPASQLATLALARFLQEYHHGSVDLGK
                     YKQPSRSPRYDWNLVFPDELIASARRKKRD"
     gene            complement(5116..5883)
                     /locus_tag="P5516_05065"
     CDS             complement(5116..5883)
                     /locus_tag="P5516_05065"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRT91843.1"
                     /translation="MIFAFANQKGGVGKTTTAVTLADGLARMKKRTLLVDLDPQGHLA
                     LSFGLEKSPGLYRLVCLDEPLSSVVVPVRPNLNLVAGDKQTEKVKRQITLADFRETIL
                     SDILASSAYDAVLLDLAPSLDVLHINGLIAADWVVIPTRLDALAVDGVKEILTTMAEI
                     GRNGHSYRGYSILPTFFDRTTRETLLQFEELTRVFGASVWPPVPQDTRVRETAAYGQT
                     LWEYSPRSTSIEGFKDGRQRLGGYRQVLARVLEVLDA"
     gene            6276..7181
                     /locus_tag="P5516_05070"
     CDS             6276..7181
                     /locus_tag="P5516_05070"
                     /inference="COORDINATES: protein motif:HMM:NF024242.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HRT91844.1"
                     /translation="MNLQENIEAVHRMQECIDTHLKERITLRMLAEAAGYSPWHAAKI
                     FKELTGKTPFDYIRTLRLSRAAVRLREEEIKVIDVAFDFLFDSHEGFTRAFSKHFGIT
                     PKNYSLHKPLTRFFIPEDVREKYFRLGKGEGMKKEDLIPNTIFVQVVKRQERKLILKR
                     GVKATDYYEYCEEVGCEVWDVLTGIKQASYEPMGMWLPENLRKPGTSEYCQGVEVPLT
                     YAEPLPEGFEMITLPACMMMIFQGQPYDDENFEQAISDLWAAMKHYKPEIYGFRWADE
                     DGPRFQLEPQGYRGYIEGRPVRALF"
     gene            complement(7280..8422)
                     /locus_tag="P5516_05075"
     CDS             complement(7280..8422)
                     /locus_tag="P5516_05075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91845.1"
                     /translation="MPPKKRFSAFDDLEKKGSKKPKIDPPAQKPSADTASKLVERIER
                     LKPSQMFPDRFQPRRLLPAEIRADFYSGRINCYQAALEWLSLARTDPGMELEIQKLIA
                     MGSSFDEHGQIKSITGKWALGNKGEYVFLIETGERRFWAACLQYASSGAETEPLLRVE
                     VVEKPTRQRQVLENRHAEPPSAVEQACEVAALILAEMGIQPDADLADDYDYFRIARTQ
                     RMPVGLWDKITPVMQLTRPRMVQLLNILQLPTSQLDLADRYRLSERVLREILSSPRDH
                     WERMVRLSIQNQLTSEEIAEIVTPATEPPSASRRPVAPILPEPGRQAARSLRRFVQTL
                     NELDRMGQDQALDEIANTMVVRGLGAQSLNLLEELARLIRARLDRR"
     gene            complement(8427..9284)
                     /locus_tag="P5516_05080"
     CDS             complement(8427..9284)
                     /locus_tag="P5516_05080"
                     /inference="COORDINATES: protein motif:HMM:NF013793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HRT91846.1"
                     /translation="MAITITIANEKGGVGKTTTAVNLAAGLALRLSERADGSGRVLLV
                     DMDPQVHALLAVGFDKHADTPEASLAALLTETPPPSIQPLIQRSDHHPNLFFVPSERT
                     AMVRAARELPALMANETRLQRALLAVQQEYAYIIIDTPPTTGDLLINALVAADHVIIP
                     VETSYLGVSGLIELQRTIEQVQTHFKPDLNVLGFLPTLCEEQRSETREILEEMRKRYK
                     KLLLPAIHKSSDLAYAHSSHMDVFTYRPPRNAGSQNIVSSSRPTREYAELTDLVLKRT
                     GARPGARGG"
     gene            9482..10300
                     /locus_tag="P5516_05085"
     CDS             9482..10300
                     /locus_tag="P5516_05085"
                     /inference="COORDINATES: protein motif:HMM:NF024793.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT91847.1"
                     /translation="MGDVMNDDEWRQVRLDRLGLKEDPFTLSADPRYLYLGPEHLAVY
                     RQAQGVISRRRGLALISGDMGMGKSSLARRLYDVYASEEMVDICYIHTAAFKSAMDAA
                     RQISETLGMPRLRSFQLQMEALEKFMASAYTANHNVVVLLDDAQLMEPEALEVLHRLY
                     NFDYDAKVAQVLAFGQSEMSGVFELNKAVNSRVFVRLALPPLTLSSALQMVLFRLSVA
                     GRSTPLINDDAFEVLYEKSQGVPREIIRLCALSMDHLLESDESMINLEIARKVN"
     gene            10303..10632
                     /locus_tag="P5516_05090"
     CDS             10303..10632
                     /locus_tag="P5516_05090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91848.1"
                     /translation="MAAIQNPNRNAWVQLYEARRIPSEPEGRAPGRPPGIVPRRKVGM
                     TLSQGEITELENWRQRLSGLLRRSVSAGETIGILTRVCTARYSRLQDQDDIDSLAQLV
                     EKLIGEE"
     gene            10793..11683
                     /locus_tag="P5516_05095"
     CDS             10793..11683
                     /locus_tag="P5516_05095"
                     /inference="COORDINATES: protein motif:HMM:NF023069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HRT91849.1"
                     /translation="MDKGMQGQSLGIDKPDYQQAWEMVLGELRAEMSRALYETWVQPL
                     KAVGYEDGTFTVGAYNAYGRDWVEGRLGARITRLLEGLYGGQVTLNVTVVNGFYRPDR
                     GEGGGKKVLSQNLINNRSTPEETTEAEENNTARAADLAGAGRSRKIMLQRAYGSERAK
                     LIQPERGMFVTGYLFNNWLPLLGHSAFTIIMAARAMCYWNPMTGELRNVVETEMGELA
                     ERAAVSVRTVKDVLNAELVQRYFLRYRVRRVMTPNGVRTAGISLQVRMDDPLTPLDQV
                     KTGLLEEEEWYLPEFSGEKE"
     gene            complement(11777..12403)
                     /locus_tag="P5516_05100"
     CDS             complement(11777..12403)
                     /locus_tag="P5516_05100"
                     /inference="COORDINATES: protein motif:HMM:NF014713.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HRT91850.1"
                     /translation="MVFLGGLLTGIGPCNVAMVPLMVGYIGGSRELPRRRAFFISLFF
                     TLGLAVTFMLLGVLAAWLGGLLGGGTRLWYYILAAVCILIGLQMLGVLGFSSPAWLEG
                     ARQLVNSRGLGGAFLLGLVSGLVVSQCATPVLAVILTFVMAQRSGLLYGAVLLLLYAL
                     GCGLPLVLVGTFTAALKNLRALSQWTIVINKISGVLLILVGFYFLWVA"
     gene            complement(12451..12894)
                     /locus_tag="P5516_05105"
     CDS             complement(12451..12894)
                     /locus_tag="P5516_05105"
                     /inference="COORDINATES: protein motif:HMM:NF012314.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HRT91851.1"
                     /translation="MKPEISPQTRSILITLGVILFVLLVALFKTSSAQRGWLRLRPVS
                     EQVATSKAAGQALVVYFHSPGCSSCARVQTALEQVYPEFESDIMLLDVDVTNRSERSF
                     VETSGVITTPTLLFISPDGRENLFVGEISTAQLRAELLALTGGRP"
     gene            complement(12988..14313)
                     /locus_tag="P5516_05110"
     CDS             complement(12988..14313)
                     /locus_tag="P5516_05110"
                     /inference="COORDINATES: protein motif:HMM:NF015451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacetone oxidase family FAD-binding enzyme"
                     /protein_id="HRT91852.1"
                     /translation="MPKDYLQINSSADPPPPLAQRSVRTSSELTTTRLLRYNILMTRE
                     LTIIGAGPAGMTAALFAARSGARVTLIDGNPVVGRKLLVSGAGRANLTNRGLAAARYA
                     CSDPAWLADVLSHFGYDEAIHFFESIGVLTYCTPDGWCYPLSESAQTVVSAFNAALES
                     AGVRLLLDHKVTSMRKMQQGFEIGFKTQRPLTSPALLVAAGGAAYPTLGSRGQLFPLL
                     EELGHHIIPPRPALAPLTCDMTPWRTLQGVRLDAAVRLFAGSQLLAETTGNLIFTAWG
                     LNGPAVMDLSHHVSARPGADLRLELNPLFASEGALRALLRDQRHSETPLNVLLGAALP
                     PKLPPLVLERLGFAADTRIRDVSDQALTRLFALLTALPFTVSGVRGFEYCQVSAGGVP
                     VNEVDPLTMRSRLLPGLQLAGEILDVTGPCGGYNLHFAFASGAIAGMNT"
     gene            14379..14642
                     /locus_tag="P5516_05115"
     CDS             14379..14642
                     /locus_tag="P5516_05115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91853.1"
                     /translation="MKKENLYRTQVLLEPGQYRELLNLAEREGISLSALLRRIVAQGL
                     EERRRQLLAQAAEKMMDLYYTDGDMTAYAALQGEDFSGGAGQA"
     gene            14639..14974
                     /locus_tag="P5516_05120"
     CDS             14639..14974
                     /locus_tag="P5516_05120"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014505.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HRT91854.1"
                     /translation="MNRGEVWWLRLDHGTPKTAVRERLVLILSTDALAVLPLRLVVPL
                     VAWREDFSTTPWLVRVPPVLNSGLEEVMAADALQVRSVSTARLSTRLGSLPERVTALV
                     AQAVAEITR"
     assembly_gap    14981..15080
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(15159..16658)
                     /locus_tag="P5516_05125"
     CDS             complement(15159..16658)
                     /locus_tag="P5516_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRT91855.1"
                     /translation="MADYDVVVIGAGLGGLSAGALLAKQGRRVLVLEQSQLIGGCCST
                     FERKGYRFDVGASIVEIIEPMERVFETLGTSLQKEVDLIACDPIMSFLFPDGERVTLP
                     LSAEATGEIISSLSPEDGARWPAFCAFADELMRTTLDTFFQMPASTLADMGALIKKNP
                     RFLKFLPVFLSSYQDVLHRYFKDEKVLKLMAYQSLYFGLPPNLVPGAYAMVPYTEHVG
                     IYYPRGGMIRIPEALQRVGGLHGMGVRFGARVTKVLVENGRARGVRLADGSEISADIV
                     VSDINAKTLYLNLIGEEHLPSCVARGVKSYKLSLSVPMIYLALDSSPELEAHHSIIAA
                     SPEDMNRYYYDQLIPGRLNDEPFGLICWPTHNDKSLAPRGKHVLNLIPQGFYHLENTD
                     WDSEKPRFIERTIESLSRRAIPGLKQRVVFAECATPLDFERDLLLPEGAIYCLQQDLA
                     AQAVFRPASKSKSIGGLYLVGSSTHPGGGVPSTIASGYIASNLIQEYED"
     assembly_gap    17017..17116
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <17117..17599
                     /locus_tag="P5516_05130"
     CDS             <17117..17599
                     /locus_tag="P5516_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRT91856.1"
                     /translation="SEGIKTHWQRSVLLVLAITLHNFPEGLAVGVAFGAVAAGVPSAN
                     LASALALAIGIGLQNFPEGMAVSMPLRGEGISQMKAFMLGQASGLVEPVAGVLGALAV
                     ISMQRILPYALSFAAGAMIYVVVEELIPESQRGESTDVATLGAMVGFAVMTFLDVALG
                     "
     gene            17628..17876
                     /locus_tag="P5516_05135"
     CDS             17628..17876
                     /locus_tag="P5516_05135"
                     /inference="COORDINATES: protein motif:HMM:NF012759.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRT91857.1"
                     /translation="MKEEIIAQLAAHITQDILRDPGRALSADEPLISSGIIDSFNLVD
                     LAVYIEDTFGAHLDDMELNADHFDTLNELADLIAGRMS"
     gene            17999..19105
                     /locus_tag="P5516_05140"
     CDS             17999..19105
                     /locus_tag="P5516_05140"
                     /inference="COORDINATES: protein motif:HMM:NF014164.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HRT91858.1"
                     /translation="MDIRDRKLAQLITQYSIGVKPGDRVVIRAGVQAEPLLKELYRCI
                     LEAGAHPTLWPELGWPEELLFNLGSDEQISYIHKTTALYYEEYDCLIRVLSEQNTKHL
                     NGVDPRKFARFTQSRGKLMNTYMDRAASGAFRWALTLYPTNAYAQDADMSLDDYADFV
                     YRACMPDMEDPVGYWRGVSAQQARIVDWLKGKKKVHLLGKDTDLRLSIEGRTFINCDC
                     RVNIPDGEIFTGPVEDSMEGHVTFSYPTIYNGREVTGVYLEFEKGRVVKASAQKNEEF
                     LLSTLDTDAGARGVGEFAIGTNVGIQQFTRQILFDEKIGGSYHMALGKGYPESGSVAQ
                     SAIHWDMICDLRDGGQIWVDDHLLYENGRFVIEG"
     gene            19425..22148
                     /locus_tag="P5516_05145"
     CDS             19425..22148
                     /locus_tag="P5516_05145"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRT91859.1"
                     /translation="MKNTPVKLTFLLSLLLALFIGGCTVLPETAEPEEGQLPLPKSLS
                     SAAEIMLSNQIQEEAAARDDVLAFIVYRVVVDSVDFSKDGRQALAWLALVDKETGYVQ
                     PGEPGLVIAQKTDDPQKPWHLTFQADQNFAEVLLALPDELLSMESKEHYLPGMQKEQK
                     AGQVFTGYRLPWPKGQKVRVSGSIGHVYTYKSCPSSCLYAFDFANGTMFDIVAARQGT
                     VKYAVWKYENGNPKDANYIVLEDTTTNPTTYQVYLHLAQNSIPPELRKVGAKVVQGQF
                     IGKADDTGYSTGHHLHFHVHTNPESWWGKSVDIVFEDVSVNGGRPRTCAEAAAFPQFG
                     KECTSGNFLVSNNGDSAPPTGGITSPEAGTKLTAPTLNVSGWMKDDIAVQSGQLFYKT
                     VGDWKPIGQTITQQPFSTTIDLCEARIPNGNFSLGLVVTDRAGNVSKLQGKVDLTKKY
                     SCPPLPPVCTPAADEVALHNDTEFQGKCQLLKMGDYADLSGLEQVKSDQALSIQVGAG
                     VSALAYSEPNFAGTMELFQDGDDNLANNLIGAGSISSIKVVKRIVMPTPVNITLPEVI
                     TDDTFLVLLWTVEEGTESSARLSGAKGFLRTVDWQNTGEWQVGTLPAGDYKITVEARN
                     LLGSATTTQEFSVVPAQAPPTTRMNALPQTSASTAIRLGWTVESPADEVERFELQVRE
                     GQGDWKDYPEAIGADARSLVYWGKQGQVYEFRMRALNKLGAAEAYPEQAEVYTLVLAD
                     CAGDEGEGTGDNLRENAPLVRSNATVTHNWCPAGDVDWVRFDAKEGDSFVLRAEPRAA
                     AAGAVIQLYAAGSQALLGEARPFNVDGPSTLNWVAPHTGVYVVRLSPADARIAGVEAV
                     YAFTVEKKSSVKPLWFIILSAIISLLFGGGYATAKKVQKTLKQKKNRRVGW"
     gene            22289..23077
                     /locus_tag="P5516_05150"
     CDS             22289..23077
                     /locus_tag="P5516_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014003.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HRT91860.1"
                     /translation="MITLTTDFGDKDGFVGAMKGVIWGICPEARIADISHQVSPQNVL
                     EAAIVLQRAAPYFPPGTVHIAVVDPGVGTQRRALAARIGAQYFIGPDNGLFTPLIADA
                     RDAGLETIFVSLEQPRYWLPRVSRTFHGRDIFAPSGAHLAAGVRLEELGRLITDPVLL
                     PLVQPEKITGGYRARISAIDTFGNLATNLAWSRVAGKALLISLKGRLIHGVSSAYGQH
                     PSGELIALVDSEDFIEVAVVNGSAAALLGAQVGDTLEVLFTDAE"
     gene            23198..23758
                     /locus_tag="P5516_05155"
     CDS             23198..23758
                     /locus_tag="P5516_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ECF-type riboflavin transporter
                     substrate-binding protein"
                     /protein_id="HRT91861.1"
                     /translation="MKGIFKVTTRTIVAAGLGAALFALLFMFVKIPSPVPGTYIQTAY
                     GLAGFFGVLFGPIAAGLLSFIGHAISDAVQYGSPWWSWVISSGIAGFIYGFAFKRTRV
                     EEGIFGGKDILTYNIIQVIGNAIAWILVAPVLDIVIYAEPANKVFAQGLMAFASNAVV
                     AGVVGTLLLLAYAATRTKKGSLSKKS"
     gene            23925..25634
                     /locus_tag="P5516_05160"
     CDS             23925..25634
                     /locus_tag="P5516_05160"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91862.1"
                     /translation="MKQPNPIIEFDHFSFQYYSQAEPTLRDINLKIYPGEKILIVGPS
                     GSGKSTLGHCLNGLIPFYYKGSIEGSLKINGIETRNLDIFKLSKMLGTVLQDSDGQFV
                     GLTVGEDIAFAMENDCVPYAEMHTRVDRVAEMVDIAHVKNLSPYELSGGQKQRTALAG
                     VLIDNVDILLFDEPLANLDPATGKTAIEIINDIHQRMGKTILIIEHRLEDVLHRAVDR
                     VIVINEGRIIADMNAHELVSSNVLTSNGIREPLYVTALKYAGVQVTPEMKPGYIWSLD
                     TSGVNESLRRWNRSVVTPPPAPPGPALLTIDHLSFSYDGVRQTLEEINFTITEGEMVS
                     IVGKNGAGKSTLAKLMCGFEKVDKGAILYKGENIRHKSIKERAEFIGFVMQNPNQMIS
                     KNLIFDEIALGLRLRGVAEEEIKERVEQVLEVCGLAPFIDWPISALSYGQKKRVTIAS
                     ILILRPNILILDEPTAGQDYRHYSEIMEFLRQINETGISIILITHDMHLMLEYTPRAI
                     VLSEGRLITDAPASVVLTTQQVIEQANLKETSLYRLAQLAGIEDGTQFVQHFIDHEKV
                     AAR"
     gene            25631..26464
                     /locus_tag="P5516_05165"
     CDS             25631..26464
                     /locus_tag="P5516_05165"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HRT91863.1"
                     /translation="MKTKLFSYNYENTPIHNLSGLIKFICFLLLTFATMYTYDIRVIL
                     GMLVFSVIILRISRIRFSQIKVMLIYVLIFIFINAIFTFFFSPEHGVTLYHTRHQIAP
                     LWGNKALTQEQLLYQVTLAFKYLSVVPLGIIFLLTTNPSEFASSVSGVGVPYKASFAV
                     SLTLRYFPDILRVYNDISQAQQARGLEMSVKAKMKERVKNSLMIIIPLIFSTLERIEV
                     VTNAMDLRGFGKEKRRTWYCVQKLRPADYIALAVSIVVTLIVAAVAYFINHGRYFNPF
                     I"
     gene            26674..28311
                     /locus_tag="P5516_05170"
     CDS             26674..28311
                     /locus_tag="P5516_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRT91864.1"
                     /translation="MKKTVVAAALGECVHVAGVVNFLRLAETSGWRTVFLGPAVGVKE
                     LLAAAKREQADLVGVSYRLTPETGERLLAEFAEEADELRAQGVKFYFGGTPPVAERAR
                     RLEFFTRVFDGSEEIEDVLASLKGEREDNTPKTGFPTTVLERIRWKAPYPVFRHHFGL
                     PTMEATLDGVSQIAEARVLDVISLGVDQDAQENFFHPERQNPRRTGAGGVPVRTVQDY
                     QALYQASRRGNYPLLRTYSGTDDFIRLAEVFTETIHNAWCAIPLFWFNQMDGRGPWDV
                     AGSIREHQRVMAWYGARGIPVELNEPHHWGMRDAPDVVCVVSAYLAAYNAKASGVRDY
                     IAQLMFNSPPGLSDAMDLAKMAAMLRIIAPLEDETFHIWRQTRTGLLSYPLQPEAARA
                     HLAASIYLQMSLRPHIVHIVGHTEAHHAATAQDVIEAARMARRAVENALRGAPDMLAD
                     TAVQKRVAELVNEAQVTLAAIQTLAGTDVPDPLTDPYTLAQALKRGILDAPQLRNNRF
                     ARGELRVAILNGACVAIDGNGRVIGEAARLEGLTRKE"
     gene            28316..29422
                     /locus_tag="P5516_05175"
     CDS             28316..29422
                     /locus_tag="P5516_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD/NADP octopine/nopaline dehydrogenase family
                     protein"
                     /protein_id="HRT91865.1"
                     /translation="MTKTKFTVIGAGHGGKAMAAHLGLLGFSTRLYNRSMEHIAAIKE
                     LGGIDLAGLNGERREFGRLELVTDNMEQALDGSDMIMVVVPSSAHAELARHMAPHLKD
                     GQVIVLHPGRTCGAIEVAKVLRDNHCTADVTVSEAETFIYASRSDGPAQARIFRMKEA
                     VPLAALPATRNRLVLDCIHEAYPQFIDGGNVLQTGLNNMGSIFHPALTLLNAGWIETT
                     HGDYQFYIDGVSPSVARVLEVLDRERVTVGSALGLRTRNAMEWLKLAYDTSGADLYEA
                     IHNQEGYYGIKAPPTLNHRYIFEDVPCSLVPIASLGDRFGVSVRAMDAVVTLANIVHR
                     TDYWRRGRTVDKLGFSTLSVSELTAYVNEGIREE"
     gene            29427..30107
                     /locus_tag="P5516_05180"
     CDS             29427..30107
                     /locus_tag="P5516_05180"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRT91866.1"
                     /translation="MNPFKLSPSMLTFFWEECPRCFYLQVVRGINRPPTAFPKVFGRI
                     DSLMKQLYADQPTSFISDTLPAGRVSLQGKWVSSEPIHFEGIAASCYLKGAFDSVLSF
                     DDGSYAVIDFKTSRPSPGHVQFYGRQLHAYAYALEHSAPGALRLSPVTRLGLVYMDPV
                     EIDYHPDDQRIFYGGEVTWSEIPLAMEPFEEFLAGVLRVLAAPEPPPANPDCGFCAYR
                     EEARAHGM"
     gene            30338..31480
                     /locus_tag="P5516_05185"
     CDS             30338..31480
                     /locus_tag="P5516_05185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91867.1"
                     /translation="MMFDIGHILKRAWGILWNYRVLWVFALLLALSGGARGGSGGGGG
                     GGSFNLPATGSNFFENGGEGWLQPGGETAQWFNELEQWADSHILPLFATEETAIRSVI
                     AIVAILMGVSLLIGLLLALVRYPSETAVMRLADAHEQSGTKVKFKEAWRLGWNERAWR
                     VFLVDLLIGAPAFTLVMMLVGLVVWMAYTLMKTAAIQLAAPAVIGIILIALFFVAFAI
                     FMVFVGFLRQYVARYAAIDGLGVGASFKQGWALFKAQFKHTFLLGLVLIGVGLAFGFA
                     MLLAAFLLIPAYILMALPGALVAAIPGGLVYWIASFSAPPVVAVIMAAIVAVPVFVTI
                     TFLPVSFLGGMFAVFSANAWTLAFRLLKPAHPPLPLNPGSPPQLPL"
BASE COUNT         6911 a         8519 c         9406 g         6469 t
ORIGIN      
        1 cagctggaac cgttgaattt gcacaggcgg tagtaataag tggtgccgct gctgccgggc
       61 acataggctt cgcgcacctt gccgtccgaa atggagaagt aggaatcctt gccaaaggcg
      121 ggttcctggt tactggtgga gtagtagatg cggaaaccac cggatgggtt gctgtctgac
      181 ttccaggtca tctgggtttt gccggtggcg ccgccgccca tgtaggtgat ggtgaaggcg
      241 ctcgaagggg tctccttgcc gacgctgccc cctcctccac cgccgcccac ggaaatgggc
      301 gtgagcgcgc gcgccgtggc ggtgcggtta ggcgtaggcg tgggcgcgta acgtttgaag
      361 gcgaaaaagc ccaggttgct gtagatatcg caagagtcgc tgacgtagcg gcaaacgctg
      421 acgatgtata ccttaccctg ctcgccgctg atgagggcag aacgcgcgtt cgggtcgccc
      481 acataggcgt cttcatcctt cgggtagatg ggcgcgcgct gctcggaagt ccataccagg
      541 gcgtatcccg aggggaaatt gccataagcc tcccatttga cgatggcctg cccgggggcg
      601 gtctcacgga tgtcggtaag cacgatgctg gcgtctggtg tgggcgccgg gggctgctgg
      661 ccatcgctcg ttggtgcggg agcgggcgtg tgggtgggca gggtgaccgc agtctgctca
      721 ggcggctcag gcagcgcggg ctgtgtcggc tgttcgagag gctcctgaag cgccgattgg
      781 gtcaaaatga ccgccagttt ctcctgcacc agctgctcgc tgacgggttg ctcagcgcag
      841 ccggaaagga tcaaggaaag cgtgagaaca actgccagaa ggattttgat gcgcataata
      901 cctccacccg gaattcgcgg tctttcaatt ataccgagaa tcacctcgcg cgttaccata
      961 cactcctgtt aacccgcgcg cgggcctggc gccaaagccg cggctgaaac gccctgtccg
     1021 gcagcgcggt gcgcctgatg gctcgctgca agtaagtgcg ccatcacgcc tcttcgtcct
     1081 ggtccagatg catcttgtgc agccagcggt gcacgaccgg ggcgatgaat acccccgcgg
     1141 cgaccaggaa gatgacgccg gaaaacagcg cgtaaagcga agcgaaaacc ttgccagcgg
     1201 cggtggcgag ggtgttcacc gggcccatac cgccaaggat catcgaggcg ttcaaaaccg
     1261 agtccagcca gggcatgttt tcagtgaagt ggtaacccag cacgccaatg gccagtgacc
     1321 ccaaaatgag cccggtgcca aagccaaaat gcctgagcag gcggaagagg aactgcctct
     1381 ttggcagcag cggctggttg tgtctctcgt acttgaagag cggcgcggag cgtttcacag
     1441 gttaattgta accattctgc gcgcccgcac aacccttatt tcaaccgggt tctgcgcccc
     1501 gctcaacccc tgcgcggcgc gccgcgttac cggctcacac cagcacagac agcatcgcgc
     1561 tggtgaataa aaccgccgcc gccagcaccg cgttgaggat gatcaggagc atgctcagtt
     1621 tcatctgcgc tgaagaaagg ctttccattt tccgccccag ggcaatgctg cggtaaaagg
     1681 cgatcgcgat catcacgatg accaggatgt gcttgacaga caaccaggcc gaatacgagt
     1741 tattgaacgt gaagaactgc cccggtccag cggcgtggcg gctcatcagc acaccggtca
     1801 cgatcaaccc gactatgcaa acatgcaccc accgcccctg ctgcctttga aacgcgcgca
     1861 tgaactgctt caactgcgca cccggtgcga aggtcttttg cagcagcggc aggtacgtga
     1921 tcaccgtcac aatcaaaccg cccatccaga ccacggtaaa aaggtcatgc aggaacctga
     1981 ccaacacagc aaccaagagt cttccatcca tctaacgtct ccttcttttt catgcatcct
     2041 aaaccaaggc gtgagcccat gctttatcaa aagtcaaacc tgtggtgaaa aggagcttat
     2101 tgcaggatta cggtttttcc ggtagaattc gccaaatgaa cgaccgttcg caaaaccaag
     2161 gcaaagccaa tcccgtgcgt tcgcgcgccc aaatcatcgg cgcgtcgcac cgcctcttct
     2221 tgatgcaggg tttccacggc accaccatgc gccagattgc caaagaatcc ggcatggcgc
     2281 tgggatcgct atataaccac ttcaccgaca aggaagcgct tttcagtgct gtcttcgaag
     2341 catacaatcc ctatcccacc ctgctgctca cgctggagaa ctcgcaagga gaaaacgccg
     2401 ccgccctggc gcgaaccgcc gcgcgccgcc tggtgaacgc cctctccgcc cgcccggacc
     2461 tggtgaaact ggtgttcatt gacgtggtcg agttccacgg cgagcatctg cgccaggcgt
     2521 atcccgccgt ggcgccgggc atgctcaatt tcgtgcggcg gctgcgaaaa gaccccnnnn
     2581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2641 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngaag catggcagcg catccagctg
     2701 gcgcccgggt tggagctgca cgttcgtcaa cccgtccatg cgaacctgca gcatctcatc
     2761 gaccagataa tcaaattgtt ccacccataa catccacttt caaaaggagg gtcattacca
     2821 tgaaaaaaac tgaattaaag ttaaagaacg acgttgcgct catccaccgt ggaaagccaa
     2881 ccaagcgtgt tctggaggta gagctaaccg cgcctgaatc ttctggcgct cgagacgcaa
     2941 ccgcgctcaa cctggcgctg atcatcgacc gcagcggctc gatggcgggc ggaaaactgg
     3001 aacaggtcaa ggcggcagtg ctgcagatcc ttgatctgct gcgtcccgtt gacagcgtcg
     3061 ccatcgttga ttacgaccac caggtaagca tcactgcaga gagcggttcg gtcacgccga
     3121 gcgcgcggga agaaatgaag caagccgtga gtagcctgca cccacgcggc atgacggacc
     3181 tgggcggcgg ctggctgtgc ggctgtgaac gcctggccca ccaccaatcc aatggaagga
     3241 tcacccgcgc tttgctgttg actgacgggt tggccaacca aggcatcact catccgctag
     3301 agttgcagca gcacgcctcc gcgctgtttg agcggggcat cgccacttcc accttcggcg
     3361 tgggagaggg ttttaatgaa cacctgctcg aaggcatggc caaccgcggc ggcggtaact
     3421 tttattatat tgaatctcgc gagcagatac ctgaactgtt gttgcaagaa ttcaaagacc
     3481 tggttgctgt gagcctgaaa agggtgaacc tggaactcac cttccctgcc ggggtggcgt
     3541 ttgaactatt tggtgattgg cgcgtggaaa aaagcgatga tcgtcacctg gtcatcaacc
     3601 tgtcagacct gtcgtctggg cgcgcggtca accttttctt aagcctgctc accccaccgg
     3661 gtgatggcga gctggtcatt catgcgtcta tcaccggaat ggatgaagcg gataagccag
     3721 tccatattga aggcgcctta cccctgcggt acgccgacgc tgctgaagtg gcgttggctg
     3781 aagaaggacg cgaccaggat atgctcaagc gttattcctc ggtagtgatc ggtcagggcg
     3841 ccagccaggc gcttaaattg gagcgagaag gtaaatttgc tgaagcgggc aaattgatgg
     3901 accagttaat gctagaacac caggactacc tgccgacgat gacccgtgaa cgctaccaac
     3961 tcatccgcga agaaatacgt gtagggttgg atgagccgcg gcgtaagaga taccagaacg
     4021 acagttacat cctcaagcgc catcggcaca tcgaccaaca gggtgaacct ggtaaataag
     4081 cacacaccac caacagaacc ggacaagtcg cgctcaaaca agcccggaac aaacccgggt
     4141 tgagcgaggg gaaaaccggt catctcaagg ccgttgactg gatagtcaac ggccttgaaa
     4201 attgaacgat cgttcacctt attcctgata acctgttgtt cagaggaata ccggcgttta
     4261 caacaattta tctacgcgca ttcttcgcgc aagcccttgc cggacctgcg ggtttgtaat
     4321 catcctggcg cttatcgaga gatggtgttc ctgcatggga aagccgtagt aaatcatgat
     4381 ctccaggcgc atccctctct gaggtgccag taatggcgaa cataaatact cgctggtttc
     4441 cttaataacc caagcgcctg aatgcaatga attcactctc gcgtgcaggc aatatttgct
     4501 cgttcgcgtg aaggcagttt ttacctcctc gcgtgcaggc aaattttgca atttggcctg
     4561 agtggaaatt ctacccgcag gcgtaagtga aacgattgcc tgatgggcgc cgggatgcaa
     4621 aattcacctt tctatagcgt gcagtaaatg cacccctggt gcgtgcaaaa gttgcacgtg
     4681 attctattca ctacttaatt aacgacttaa ctgactttaa gagcttaatc cctttttttg
     4741 cggcgtgctg atgcgatcag ctcatctggg aaaaccaggt tccagtcata gcgcgggctt
     4801 ctggatggtt gtttatattt ccccagatcc accgagccgt ggtgatactc ttgcaggaag
     4861 cgcgccaacg ccagggtagc aagctggctg gctggaagac gtaactcttc tgccagcaaa
     4921 cgcagccctt cgcggatgga gggcggcagg tcgtacgtgg cgcgctggtc acggcgcgcc
     4981 tggatcttcg cccgttcgcg cgagatgcgc tcgcgttcac ggcgcggcaa ctgcgcttcc
     5041 gattgttttt gctgcattcc cttcaacagg tccgccaccg cggggtccag cgccgaacgg
     5101 cgttgttttt tatcctcagg catcgagcac ctccagcaca cgggcgagca cctggcggta
     5161 accacccagg cgctgacgtc catccttgaa tccctcaatc gaagtggaac gaggggaata
     5221 ttcccatagc gtctgaccgt aagcagccgt ttcacgcacg cgcgtatcct gtggcacagg
     5281 gggccaaacg ctggcgccga acacacgggt gagttcctca aattgcaaca gcgtctcgcg
     5341 ggtggtgcgg tcaaaaaagg tgggcaaaat actgtaaccg cggtaagagt gaccgttgcg
     5401 gccaatctcc gccatggtgg tgaggatttc tttaacccca tccaccgcca gcgcgtccag
     5461 gcgggttgga atcaccaccc aatccgcggc gatcagaccg ttaatgtgca gcacatccag
     5521 cgagggcgct aaatccaaca gcaccgcgtc ataagcggaa ctggcgagaa tatcgctcag
     5581 gatggtctcg cggaaatctg ccagtgtaat ctggcgcttg accttttcgg tctgtttgtc
     5641 acccgccaca aggttcaggt tggggcgcac cggtacaacc accgaagaga gcggttcgtc
     5701 caggcagaca aggcggtaaa gacccggtga tttctcaaga ccaaaagaaa gcgccaggtg
     5761 cccctgtgga tccaggtcca ccagcagcgt acgctttttc atgcgcgcca acccgtcagc
     5821 cagagtgacc gcggtggtgg tctttcccac gccgcctttc tgattggcaa aagcaaaaat
     5881 cataggaaat atacctctcc agacgaaccg tgtttttggc taagaaaaaa ggtaaaaacc
     5941 aaaaacaagg gtcgccccat gagattatac agggtcaccc cttgaaaaac aagggtcgcc
     6001 cctggagaaa tgacaagctc aactgcaacg tcaccccttg agacaaccaa agccccttca
     6061 acaagtgaat atatggaaaa tgaccatcat ataggcaaga atgtattttt gttcaggggc
     6121 aagggcttga aaagtgtaac catggttaca cttcgtgttt aaacgggctg ctggattact
     6181 cactttattg gtaatgaaaa cgttcgccca gcacaaaaaa ggtcaagcaa attcctcatc
     6241 caaatgatac gcttacagga ataaagggtt atggcatgaa cctccaggaa aatattgaag
     6301 cggtacacag aatgcaggag tgcatagaca cgcatctgaa agagcggatc accctgcgta
     6361 tgctggcaga agcggcagga tattcgccat ggcacgctgc gaaaatattc aaggaattga
     6421 caggaaagac cccttttgat tatatccgca cactgaggtt gtctcgagct gccgtcagac
     6481 tacgggagga ggagatcaaa gtgattgatg ttgcttttga tttccttttt gattctcacg
     6541 aagggttcac gcgggcattt tcaaaacact ttggcatcac accgaagaat tacagcctgc
     6601 acaagccact gacgaggttt ttcataccag aggacgtccg tgagaaatat tttcgtcttg
     6661 gaaaaggaga aggcatgaaa aaagaagatt tgatacccaa tacaatattt gtccaggtcg
     6721 tcaaacgaca agaacgaaag ctaatcctca agcgcggcgt gaaggcgacg gattactacg
     6781 aatactgcga ggaggttggc tgcgaagtgt gggacgtgct caccggcatc aaacaagcga
     6841 gctatgaacc catgggtatg tggttgccgg agaacctgcg caagccaggc acatcggagt
     6901 attgccaggg ggtggaagtg ccgctgacct acgcagaacc gctgccggag gggtttgaaa
     6961 tgatcaccct gcccgcctgt atgatgatga tcttccaggg tcagccatac gatgacgaga
     7021 acttcgaaca agccatcagc gatctgtggg cggcgatgaa gcactataag ccggagattt
     7081 atggattccg ctgggcagat gaggacggac cacgctttca actcgaaccg caggggtacc
     7141 gggggtatat cgagggacgg ccggttagag ccctgttttg acggtaagga agacttttaa
     7201 cgctgatgtt acaggagagc gcgaagtgta accacggtta cactttaaga cgacaaattt
     7261 tggaaatgat acgaatcgat taacgtcgat ccaggcgggc gcggatcagg cgggcaagct
     7321 cttcgagcag gttcagcgat tgcgctccca gccctctgac caccatggtg ttggcaatct
     7381 catccagcgc ctggtcctgc cccatgcggt cgagttcgtt caacgtctgg acaaaacgcc
     7441 gcagcgaacg cgctgcctgg cgaccaggtt cgggcaggat gggtgcgact ggacgccggg
     7501 atgcggatgg tggttccgtg gcgggagtga caatctccgc gatttcttca gaggtgagct
     7561 ggttctggat gctgagccgc accatgcgtt cccagtggtc gcgcggggag gacaggatct
     7621 cacgcagcac ccgctctgaa aggcggtaac ggtctgccag gtctaactgg ctggtgggca
     7681 gttgcaggat gttgagcagt tgaaccatgc gcgggcgggt gagctgcatc accggcgtaa
     7741 ttttatccca cagacctaca ggcatgcgct gggtgcgggc gatgcggaag taatcgtaat
     7801 catccgccag gtcagcgtct ggttggatgc ccatttccgc caggatgagc gcggccactt
     7861 cacaagcctg ttccaccgca gatgggggtt ccgcgtggcg gttctccaat acctggcgtt
     7921 gacgggtggg cttttccacc acctcgacgc ggagcagggg ttctgtttca gcgccggaag
     7981 aggcgtattg caggcaggcg gcccaaaaac ggcgttcacc ggtctcaatc agaaacacat
     8041 attcaccctt gttgccaagc gcccactttc cggtgatgga tttgatctga ccgtgctcat
     8101 cgaacgaaga acccatggcg atgagctttt ggatttcgag ttccatgccg ggatcggtgc
     8161 gcgccagcga cagccattca agcgccgcct ggtagcagtt gatgcgaccg ctgtaaaaat
     8221 ccgcgcggat ttctgcaggc agcaggcggc gcggttgaaa gcgatcgggg aacatctggc
     8281 tgggtttaag gcgctcaata cgttccacca gcttggacgc cgtatctgcg gagggcttct
     8341 gagccggggg gtcgatcttg ggtttttttg aacctttttt ttccaggtca tcgaaagcgc
     8401 tgaatctttt tttaggcggc atgggcttat cctccccgcg cgccagggcg ggcgccggtg
     8461 cgtttgagaa cgaggtcggt caattcggca tattcgcgcg tggggcgcga actggaaacg
     8521 atattttggc tgccggcgtt acgcggcggg cggtaagtaa acacatccat gtggctggaa
     8581 tgagcgtaag ccaggtcgga ggatttatgg atggccggca gaagcaactt tttgtagcgt
     8641 ttacgcattt cttccaggat ctcacgcgtt tcactgcgct gttcttcaca cagggtaggc
     8701 aggaagccta aaacgttgag atcgggtttg aagtgggtct gcacctgctc gatggtgcgc
     8761 tgcagctcga tgagccctga aacgcccagg taagaggtct ccaccgggat gatgacatgg
     8821 tcagccgcga ccagcgcatt gatcagcagg tcgccggtgg tgggtggggt gtcgatgata
     8881 atatacgcgt actcttgctg taccgccaac agcgcgcgct gcaggcgggt ttcattggcc
     8941 atcagcgcgg gcagctcccg cgcggctcgc accatcgcgg tgcgctctga tggcacaaaa
     9001 aacaggttgg gatgatgatc actgcgctgg atgaggggtt gaatggaggg tggcggcgtt
     9061 tcagtgagca gggcggccag agaggcttcg ggggtgtcag cgtgtttgtc aaaccccacc
     9121 gccagcaaag cgtgaacctg ggggtccata tccaccagca gcacacggcc actgccatcg
     9181 gcgcgttctg aaaggcgcaa cgccagcccg gcagccaggt tgacagcggt ggtggttttc
     9241 ccgacaccgc ccttttcatt tgcaattgtg atggtaatcg ccatatctcc cgtcaatccg
     9301 tgagggcttg agcacacccc gcaatcaaat tatacacaat ttcattttca ctatgttagc
     9361 ataaaaaaaa ggaagttgca acaaattgcg ctaaatcctt tgaaaatcat gtaaaatatc
     9421 ataaatctat tgatttcagc ctgatttcgt gcgataatta gtgaggtatc ggaggttatc
     9481 catgggcgat gtgatgaacg atgatgaatg gcgacaggta agactcgacc ggctggggtt
     9541 aaaagaagac cccttcacgc tgagcgccga cccgcgctat ctgtacctgg gacctgaaca
     9601 cctggctgtg taccgacagg cgcagggggt catctcccgg cggcgcgggc tggcgctgat
     9661 cagcggcgat atggggatgg gaaaaagctc tctcgcccgg cgcctgtatg acgtgtacgc
     9721 ctctgaagag atggtggata tctgttatat ccacaccgcg gcatttaagt ccgccatgga
     9781 cgcggcgcgg cagatctcgg agacgcttgg tatgccgcgg ctacgctctt ttcagctgca
     9841 gatggaagcc ctggaaaagt tcatggcttc ggcttacacc gccaaccata atgtggtggt
     9901 gctgctggat gacgcgcagt tgatggaacc ggaggctctc gaggtgctgc accgcctcta
     9961 taactttgat tacgacgcca aggtggcgca ggtgctggct tttgggcaat cggaaatgtc
    10021 gggtgttttt gagctgaaca aggcggtcaa ctcgcgtgtt tttgtgcgcc tggcgctgcc
    10081 accgctcacg ctctcttccg cgctgcagat ggtgctcttc cgcctcagcg tggccgggcg
    10141 atccacaccc cttatcaacg acgacgcctt cgaggtgttg tatgaaaaat cacagggcgt
    10201 accgcgtgag atcatccgct tatgcgcgct ttcaatggac catttactgg aaagcgacga
    10261 gtcgatgatc aatctggaaa tcgcccgaaa ggtgaactga ttatggcagc catacagaac
    10321 cccaaccgga atgcctgggt acaactttac gaagcccggc gcattcccag cgagcccgaa
    10381 gggcgcgccc cgggcagacc accggggatt gtccctcgcc gcaaggtggg catgacgctc
    10441 tctcaagggg aaatcacgga gctggaaaac tggcgccaac gcctttcagg actgctgcgc
    10501 cgctcggttt ctgctggcga aaccattggt atcctcaccc gcgtgtgcac tgcgcgctat
    10561 tctcgcctgc aagatcaaga cgatattgat tcgctggcgc agctggtgga aaaactgata
    10621 ggtgaggaat gaataaattc ttccataaac ccccacaatt aatggtagaa aaaactatta
    10681 tcgttttcgc ctatcaaaac gagagaaaaa accatcttga taaaatagaa aaaaagttct
    10741 ataatcaaac caaacgcgga cgtactggac aacgccgctg atcggatgaa caatggataa
    10801 aggcatgcag gggcaatccc tcgggatcga caagccggat tatcaacaag cctgggaaat
    10861 ggtgttaggc gaactgcgcg cggaaatgtc gcgggcgctt tacgagacct gggttcaacc
    10921 gctcaaagca gttggttatg aagacggcac tttcaccgtg ggcgcctaca acgcctatgg
    10981 gcgcgactgg gtggaaggac gcctgggagc gcgcatcacg cggctgctgg aaggccttta
    11041 cggcgggcag gtcacactca atgtgacggt cgtcaacggg ttctaccgcc cggaccgcgg
    11101 ggagggaggt ggtaaaaagg ttctttcaca aaacctgata aacaaccgct cgacccctga
    11161 ggaaactaca gaagcggaag aaaacaacac cgcgcgcgcc gcagacctgg cgggcgctgg
    11221 gcgttcgcgc aagataatgc tgcagcgcgc ttacggcagc gaacgcgcca agctcatcca
    11281 gccggaacgc ggtatgttcg tcaccggtta tctcttcaac aactggctgc cgctattggg
    11341 acattcagcc ttcaccatca tcatggcggc gcgggcaatg tgctactgga acccgatgac
    11401 cggtgaactg cgcaatgtgg tggaaaccga aatgggcgaa ctggcagaac gtgccgccgt
    11461 ttctgtgcgc acggttaaag atgtgctcaa cgcggaactg gtgcagcgct actttttgcg
    11521 ctaccgcgtt cgccgcgtga tgactcccaa cggcgtgcgc accgccggca tttcactgca
    11581 agtgcgtatg gacgaccctc tgaccccgct tgaccaggtg aaaaccggct tgttagaaga
    11641 agaggaatgg tacctgccgg agttttcggg ggaaaaagaa taaaccgcca gcaccgggag
    11701 tgctcttcgg gcacgcctgc agcagcaacg ctgatgggga agggggctcg aaaaatagtg
    11761 cgtgtagtta aatcactcaa gcaacccaca agaaatagaa gcctaccagg atgagcagta
    11821 cgccgctgat cttattaatg acgatggtcc actggctgag ggcgcgcagg ttcttgagcg
    11881 cggcggtgaa ggtgcccacc agcaccagcg gcaggccgca gcccagggcg taaagcaaga
    11941 gcaagaccgc cccgtaaagc aatccggaac gctgtgccat gacaaaggtg aggatcaccg
    12001 ccaacaccgg ggtggcgcat tgagatacca ccagccctga taccagcccc agcagaaacg
    12061 ctccccccag accgcggctg ttcaccagct gacgcgcccc ctccagccag gcgggcgagc
    12121 tgaagcccag cacgcccagc atctgcaaac caatgaggat gcacaccgct gccaatatgt
    12181 agtaccacag gcgtgtacca ccgccgagca gaccacccaa ccacgccgcc aacacgccca
    12241 ataacataaa agtgacagcc agacccaggg taaaaaaaag cgagataaaa aaagcgcggc
    12301 gccgtggcag ttctctcgac ccgccaatgt accccaccat gagcggcacc atggccacgt
    12361 tgcacggtcc gatgccggtg agcaaaccgc ctaaaaagac cagcaggtag ccgagaagag
    12421 aagagggttg cagcagcgaa gggtcgatgt ttatggtcga cctcctgtca gcgccagcag
    12481 ttcagcgcgc agttgggcgg tgctgatctc accgacgaag aggttttcgc gaccgtcagg
    12541 gctgatgaag agcagggtag gggtggtgat cacgccgctg gtttcaacga acgatcgttc
    12601 actccggttg gtcacgtcca catccaacag catgatgtcg ctctcgaatt ccgggtacac
    12661 ctgttcgaga gcggtttgca cgcgggcgca ggaactgcaa cccggggagt ggaagtacac
    12721 caccagcgct tgtccggccg ctttgctggt ggccacctgt tcagacactg gtcgcaagcg
    12781 cagccaaccg cgctgcgccg agctggtctt gaacagcgcc accaacagta caaagagaat
    12841 cacaccaagg gtgatgagga tggagcgggt ttgagggctg atttcaggct tcatcaagga
    12901 acacctgcaa caggagtgga ggtaatttta cttgagaaat caggcggtgc cggtgataac
    12961 aaatcgggca gcaggcaagc aggtgaatca ggtgttcatt ccggcgattg ccccgctggc
    13021 gaaagcaaag tgcaggttat agccgccaca gggaccggtg acgtcgagaa tctcaccggc
    13081 cagttgcagc cccggcagca agcgtgagcg catggtcaga gggtcaacct cgttcacggg
    13141 cacgccgccg gcgctcacct ggcaatactc gaaaccgcgc acaccgctga cggtgaaggg
    13201 cagggcagtg agcaatgcga aaagacgggt cagcgcttga tcggagacat cgcgtatgcg
    13261 tgtatctgcg gcgaagccca ggcgttcgag caccaacggg ggcagtttgg gcggtaaggc
    13321 ggcacccagt agcacattga gcggagtctc tgaatggcgt tgatcgcgca gcagggcgcg
    13381 cagggcgcct tccgaagcga agagggggtt cagctccagg cgcaggtcag cgccgggacg
    13441 ggcggagacg tggtggctca ggtccatcac cgccggtccg ttgagtcccc aggcggtgaa
    13501 gatgaggttg ccagtggttt cagccagcag ctggctgccg gcgaagagcc tgacggcggc
    13561 gtccaggcgt acgccctgca acgtgcgcca gggggtcatg tcgcaggtaa gcggggcgag
    13621 ggctgggcgg ggtgggataa tgtgatgacc gagttcctcc aggagaggaa agagttggcc
    13681 gcgcgagccg agtgttggat aggcggcacc gccggcggct accagcaacg ccgggctggt
    13741 gagcggtcgc tgggtcttga acccgatctc gaagccctgt tgcatcttgc gcatagacgt
    13801 tactttatgg tccagcagca ggcgcactcc ggcgctttcc agggcggcgt taaaagcgct
    13861 caccacggtc tgcgcggatt cagaaagggg gtagcaccaa ccgtcaggcg tgcagtaagt
    13921 gagcacaccg atgctctcga agaaatgaat cgcctcgtcg tagccaaagt gcgataatac
    13981 atcagccagc caggcagggt cgctgcaggc gtaacgcgcg gctgccagcc cgcggttggt
    14041 gaggttggcg cgcccagcgc cgctgaccag cagcttgcgc cccaccacgg ggttgccgtc
    14101 gatcagggtg acccgcgcgc ctgaacgcgc ggcgaacagc gccgcggtca tgccggccgg
    14161 ccccgcgccg atgatggtga gttccctggt cataaggata ttgtatcgca agagccgggt
    14221 ggtggtgagt tcgctgctgg tgcgcacgga acgctgagcc aggggaggtg gaggatccgc
    14281 gctgctgttt atttgtagat aatctttcgg cattattaaa agatgtagaa aaaatatgat
    14341 aaatatattg taataataag atgtataata tagatgtaat gaagaaagaa aacctgtacc
    14401 gcacccaggt gctgctggaa ccggggcagt accgcgaatt gctcaacctg gcggagcgcg
    14461 aggggatcag cctttcagcg cttctgcggc gtatcgtggc gcagggtctg gaagagcggc
    14521 gccgccaact gctggcgcag gcggcagaga agatgatgga cctgtattac actgacggcg
    14581 acatgaccgc ttacgccgcg ctgcaaggag aagattttag cggcggggca gggcaggcgt
    14641 gaaccgcggt gaagtctggt ggctgaggtt ggatcacgga acccccaaga ctgccgtgcg
    14701 ggagcgcctg gtgctgatcc tttcgacaga cgcgctggcg gtgctgcctt tgcgcctggt
    14761 ggtaccgttg gtggcgtggc gcgaagattt ttctaccacg ccctggctgg tgcgcgtgcc
    14821 accggtgctc aacagcgggc tggaagaagt gatggcggct gacgcgctgc aggtgcgctc
    14881 tgtttccact gcgcggctca gcacgcgcct gggcagcctg ccggagaggg tgacagcgct
    14941 ggtagcgcag gctgtggcgg aaatcacgcg ttgatcttcc nnnnnnnnnn nnnnnnnnnn
    15001 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    15061 nnnnnnnnnn nnnnnnnnnn cggtaccacc aggtgatgcc gaataccagc agggctacaa
    15121 taaaaaaggt cagcaggggt ctgcgcattt tggctccttc agtcttcata ttcctggatg
    15181 aggtttgagg cgatataccc tgaagcgatg gtgctgggca ccccgccgcc ggggtgggtg
    15241 gaagatccca ccaggtacag cccgccgatg cttttactct tgctcgccgg gcggaagacc
    15301 gcctgcgcgg ccaggtcttg ctgcaggcag tagatggcgc cttcgggcag cagcaggtcg
    15361 cgctcaaaat ccagcggggt ggcgcactcg gcgaagacca cgcgctgctt aagtccgggg
    15421 atagcgcggc gtgagaggct ctcgatggtg cgttcgatga agcgcggttt ttcgctgtcc
    15481 caatctgtgt tctcaaggtg gtaaaaaccc tgcgggatga gattaagcac gtgctttccg
    15541 cgcggggcga gggatttatc gttatgcgtc ggccaacaaa taagcccgaa aggctcgtcg
    15601 ttgagccgcc cgggaatcag ctgatcataa taatagcggt tcatgtcttc gggagaggcg
    15661 gcgatgatgc tgtggtgcgc ctccagctca ggagaagagt ccagcgccag gtagatcatc
    15721 ggcacagaga gcgaaagctt gtagctcttc accccgcgcg ccacgcaaga gggcaggtgt
    15781 tcctcgccga tgaggttgag atacagcgtt ttggcattga tatcagacac cacgatatcc
    15841 gcgctgatct cgctgccgtc tgccaggcgc acgccccggg cgcggccgtt ctctaccaat
    15901 actttggtca cgcgcgcgcc gaagcgcacg cccatgccgt gcagccctcc gacgcgctga
    15961 agcgcttcag gaatacggat cataccgccg cgcgggtagt agatccccac atgctcggta
    16021 tagggcacca tggcgtaggc accaggcacc aggttgggcg gcagcccgaa gtataacgac
    16081 tggtaagcca tcaacttcag cactttctca tccttaaaat agcggtgcaa cacatcttga
    16141 taagaagaga ggaacacagg caggaacttg agaaagcgcg gattcttttt aatgagcgcg
    16201 cccatgtctg ccagtgtgct ggcgggcatc tggaagaaag tatccagggt ggtgcgcatc
    16261 agctcatccg caaaagcgca gaacgccggc cagcgcgccc cgtcctccgg agagaggctg
    16321 ctgatgatct cgccggtggc ttcggctgaa agcggcaggg tcacacgctc gccatcaggg
    16381 aaaaggaagg acatgatggg gtcgcaggcg atgagatcca cttctttctg caggctggtg
    16441 ccgagggtct caaacacgcg ctccatgggt tcgatgatct cgacgatgga agcgcctacg
    16501 tcaaaacggt aacctttacg ctcaaaagtt gaacagcaac caccgatcag ctggctttgt
    16561 tccaacacca gcacgcggcg accctgtttg gcgagcagcg ccccggcgga aagcccgccg
    16621 agacccgccc cgatgacaac gacatcataa tctgccatgg ttccctccgt ggaataggct
    16681 tgaagaatga atgggtgcga catgattatt atagatataa tcgcgcagat tggtacgaag
    16741 agttgcagca gggctgtctg accgcggggt tttttctctc ccgctgctgc ccgttcgctg
    16801 cagacggcta acctcagacg gcaactcccc cgggcgtgat tttgatttta ctgttcccga
    16861 ttgggggttc acttacacca gggcgattat aattaccaca aggtccggag gtccatttat
    16921 gcttgctttt cttaccgctt ttccgccagt gttacaggcg ctgttcgcca ccctgttcac
    16981 ctggtcggtc accgcgctgg gcgcggcttt cgtcttnnnn nnnnnnnnnn nnnnnnnnnn
    17041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    17101 nnnnnnnnnn nnnnnnagcg aaggcattaa gacccactgg cagcgctcgg tgctgttggt
    17161 gctggccatc actttgcata acttccccga gggcctggcg gtgggagtgg cattcggcgc
    17221 cgtggcggcg ggggtgccat cagccaacct cgcttcggca ctggcgctgg ccatcggcat
    17281 tgggctgcag aacttccccg aaggcatggc cgtctccatg ccgctgcgcg gcgaaggaat
    17341 ttcacaaatg aaggccttca tgctggggca ggccagtggg cttgtagaac ccgtcgccgg
    17401 cgtgctgggc gcactggcgg ttatcagtat gcagcgcatt ttgccttacg cgctcagttt
    17461 cgctgcaggc gccatgatct atgtggtggt cgaggagctc atccctgaaa gccagcgcgg
    17521 cgaaagcacc gatgttgcca ccctgggtgc catggtcggc ttcgcggtga tgaccttttt
    17581 ggatgtagcg ctggggtagt tgaattttcg taagcgatgg aggacgaatg aaagaagaga
    17641 tcattgccca actggcggcg cacatcaccc aggacatact gagagacccc gggagggcgc
    17701 tgtctgcgga tgaaccgctc atttccagcg gtatcattga ttctttcaac ctggtggacc
    17761 tggccgtgta cattgaagac accttcggcg cgcatctgga cgatatggag ctcaacgccg
    17821 accattttga cacgcttaac gaactggcgg acttgattgc cgggcgcatg agctgaacca
    17881 cgccccgcga aaaacccgtc atttgaccgg cttgtgttgc tggcgtacgc cgctgagggc
    17941 tcaacacctg cacctggcgt ataatgaatc cgcaggaaag ccaaacgagg agaaaaaaat
    18001 ggatattcgc gaccgtaaac tggcgcagct catcacgcag tattccatcg gggtcaaacc
    18061 tggtgacagg gtggtcatac gggcgggggt gcaggctgag ccgctgttga aagagcttta
    18121 ccgttgtatt ttagaggctg gcgcgcatcc cactttgtgg cccgagctgg gctggccgga
    18181 ggaattgctt ttcaacctgg gcagcgatga acagatcagc tacatccaca agaccacggc
    18241 gttgtattac gaggagtacg actgcctgat ccgggtgctt tctgaacaaa acaccaaaca
    18301 cctcaacggg gtggatccgc gtaagttcgc acgctttacc cagtcgcgcg gcaagctgat
    18361 gaacacctac atggatcgcg ccgccagcgg cgcgttccgc tgggcgctca ccctttaccc
    18421 cactaacgct tacgcccagg atgcggatat gtcattggat gactacgctg actttgtgta
    18481 ccgcgcctgc atgccagata tggaagaccc ggtgggctac tggcggggtg tctccgcgca
    18541 acaggcgcgc attgtggatt ggctcaaagg caagaagaag gtacacctgc tgggtaaaga
    18601 caccgacctg cgtttgagca tcgaagggcg cactttcatt aattgtgatt gccgcgtcaa
    18661 tattcctgat ggtgaaatct tcaccgggcc ggtggaagac agcatggagg gacatgtgac
    18721 cttctcttat cccaccatct acaatgggcg cgaggtcacc ggtgtgtacc tggaatttga
    18781 gaaggggcgc gtggtgaaag cgtccgcgca gaagaacgaa gaattcctgc tgagcacgct
    18841 ggacactgac gccggcgccc gcggggtagg tgagttcgcc atcggcacca acgtgggcat
    18901 ccagcagttc acccgccaga tactctttga cgagaagatt ggcggctctt accacatggc
    18961 gctgggcaaa ggctatcccg agtcgggcag cgtggcgcag tcggctatcc actgggatat
    19021 gatctgtgac ctgcgcgacg gcggtcagat ctgggtggat gaccacttgc tttacgagaa
    19081 cggtagattc gtcatcgaag gctgaggttc aaacgcgcaa gcaggcgcct ttgcggctgg
    19141 catcagctcc taccccatgt tgggtggagg gctgaaagac cccagagggg gcttaaaaac
    19201 tacgccgcga gcatgaacgc accatccaaa agccccagcg gggcttttga ttttaaatag
    19261 atgcaaacca taaagagagc gaggtgtgcg ccctcacttg aaaaccagga ttttgaggag
    19321 gggagatttc ggcggcagag cgctgacggt ggtatggatt tccttatcac ggcataatac
    19381 ttgcacccaa attcctctga acgttgcgcg tgacgaggtt attgatgaaa aacacacctg
    19441 tgaaactcac ttttttgctc tctttgctgc tggcgctgtt catcggcggc tgcacggtgc
    19501 tgcccgaaac agcggagccc gaagaggggc aactgccttt acccaagtcc ctttccagcg
    19561 ccgccgagat catgctctcc aaccagatcc aggaggaagc agcggcgcgc gatgatgtat
    19621 tggccttcat cgtttaccgc gtggtagtcg acagcgtgga cttttcaaaa gacggcaggc
    19681 aggcgctggc ctggctggcg ctggtagaca aagaaactgg ttatgtacag cctggcgagc
    19741 ccgggctggt gatcgcgcag aaaacggatg acccgcaaaa gccctggcac ctgacctttc
    19801 aggcggacca gaacttcgcc gaagtgttgc tggcgctgcc ggatgagttg ctctccatgg
    19861 aaagcaaaga acattacctc cccggcatgc agaaagaaca aaaagccggc caggtcttca
    19921 ccggttaccg cctgccctgg cccaaggggc aaaaggtgcg cgtcagcggt agcatcgggc
    19981 atgtgtacac ctacaagtct tgcccgtctt catgccttta tgcttttgat ttcgccaacg
    20041 gcaccatgtt cgatattgtg gccgcccggc agggcacggt gaagtacgcg gtgtggaagt
    20101 atgaaaacgg caaccccaaa gacgccaatt atattgtgct ggaagatacc accaccaacc
    20161 ccaccacata ccaggtgtac ctgcacctgg cgcagaacag catcccaccc gaattacgca
    20221 aggtgggggc gaaggtggta caggggcagt tcatcggcaa ggcggacgat actggttaca
    20281 gcacaggcca ccacctgcat tttcatgtgc acaccaaccc cgaaagctgg tggggcaagt
    20341 cggtggatat cgtttttgag gatgtgagtg ttaacggagg gcgcccgcgc acgtgcgccg
    20401 aagccgcggc ttttccccag tttggcaaag agtgcacatc aggaaatttc ctggtttcga
    20461 ataatggtga ttctgctccg ccaactggcg gcattaccag cccggaagcc ggaacgaaac
    20521 tgacggcgcc caccttgaat gtgagcggct ggatgaaaga tgatatcgcc gtgcagagcg
    20581 gtcagttatt ctataaaacg gtgggagatt ggaaacctat cggtcagacc atcacccagc
    20641 agccgttttc cactaccatt gatctgtgcg aggcgcgcat tcccaacggg aatttttccc
    20701 tggggctggt cgtaacggat cgggcgggta atgtttccaa actgcagggc aaagtagatt
    20761 taaccaagaa gtacagctgc ccgccgctgc cgccggtttg cacgcccgcg gcagacgaag
    20821 tggcgctgca taacgacacc gagttccagg ggaaatgcca attactgaaa atgggagatt
    20881 atgctgacct atcggggctg gaacaggtaa agtcagacca ggcgctctcc atccaggtgg
    20941 gggcgggggt ttcggcattg gcctatagcg aacccaactt tgccggtacc atggaactct
    21001 tccaggatgg tgatgacaac ctggcgaaca acctgattgg cgcgggcagc atttcttcta
    21061 tcaaggtggt caagcgcatt gttatgccca cacctgtaaa cattaccctg ccggaagtca
    21121 tcaccgacga caccttcctc gtcctcttat ggacggtgga agagggcacg gaatcaagcg
    21181 cgcggctgag cggagccaag ggctttctca gaacggtgga ctggcagaac accggcgaat
    21241 ggcaggtagg cacgctgccg gcgggagatt ataaaatcac cgtggaagcg cgcaatctgc
    21301 tgggcagcgc caccaccacc caggagttca gcgtggtacc cgcccaggcg cccccaacca
    21361 cgcgcatgaa cgccttgccg caaacttccg cttcgaccgc catcaggctt ggctggacgg
    21421 tcgagagccc cgctgacgaa gtagaacggt ttgagttgca ggtgcgcgag gggcagggcg
    21481 attggaaaga ttacccggaa gccatcggtg ccgacgcacg cagcctggtg tactggggta
    21541 aacaggggca ggtgtacgag ttccgcatgc gcgcgctgaa caaacttggc gcggctgagg
    21601 cttaccccga gcaggctgag gtgtataccc tggtgctggc tgactgcgca ggcgacgagg
    21661 gcgaaggaac cggggataac ctgcgcgaaa acgcgccact ggtgagaagt aacgcgacgg
    21721 tgacccataa ctggtgcccg gcgggagatg tggactgggt acgttttgat gccaaagagg
    21781 gcgacagctt tgtattgcgc gcagaaccgc gggccgcggc tgcgggggca gtcatccagt
    21841 tatacgccgc aggcagccag gcgctgctgg gcgaagcccg ccccttcaat gtggatggac
    21901 ccagcaccct caactgggtg gcgccgcaca ctggcgttta tgtggtgagg ttaagcccgg
    21961 ccgacgcgcg gatcgctggc gtggaagcag tatacgcctt caccgtggag aaaaagagca
    22021 gcgttaaacc cctgtggttc atcattttgt cggcgattat ttcactcttg tttggcggtg
    22081 gttacgcgac agccaaaaag gtgcagaaaa cgctcaaaca aaagaaaaac cgccgcgtgg
    22141 gttggtaatg caggctggtc aaaaccgagg ttggggcggt gttttcaaat cgccccactt
    22201 attatttaag ccttcttctt gacgtctggg aatgcggatg ctacagttaa ttaactttta
    22261 ccccatagat tgaggtgcac tcttgccagt gatcaccctg acgacagatt ttggtgataa
    22321 agatggcttt gtgggcgcga tgaagggcgt catctggggc atctgccccg aggcgcgcat
    22381 cgccgacatc agccatcagg tctcgccgca gaatgtttta gaagcggcca ttgtgctaca
    22441 gcgggcagcg ccttactttc cacctggaac cgtgcacatt gctgtggtgg atcctggcgt
    22501 gggcacacag cggcgcgcgc tggcagcgcg tatcggcgcg cagtacttta ttggtccgga
    22561 taatggtctg ttcacaccgc tcatcgccga cgcgcgagac gcggggttgg agaccatctt
    22621 tgtgtctctt gagcagccgc gttactggct gccgcgggtg agccgcacct ttcacggtcg
    22681 tgatattttc gcgccgtcgg gcgcccatct ggccgccggg gtgaggttgg aagagctggg
    22741 caggctcatc actgacccgg tgctgttgcc gctggtgcag cccgaaaaga tcactggcgg
    22801 ttaccgggcg cgaatctccg ccattgacac tttcggcaac ctcgccacca acctggcctg
    22861 gtcgcgggtg gctggcaaag cgctgctaat ttcactcaag ggcaggctca ttcatggggt
    22921 ttccagcgcg tatgggcagc acccttctgg agagctcatc gcgctggtgg atagtgaaga
    22981 tttcattgaa gtggcagtgg tgaacggcag cgcggcggcg ctgctgggtg cgcaggtggg
    23041 agatacactg gaggtgctct ttactgacgc ggaataagcc ctgaagagat gaaactatgg
    23101 aaggtttggg gtgttaaagt ccgtgagtca ttgacaaaaa gacgcttttt cagtatggtc
    23161 attatggcga tgatttaacc taatctgagg aggaaccatg aaaggcattt ttaaagtaac
    23221 aaccagaacc attgtggcag ccggtttggg ggcagcgctc ttcgcgcttc tgttcatgtt
    23281 cgtgaagatc ccatcaccag ttcccggtac atacatccag actgcttacg ggctggcagg
    23341 tttctttggc gtcttgtttg gaccgatagc ggccggtctg ctgtcattta tcggtcacgc
    23401 catcagcgac gcggtgcagt acggcagccc ctggtggagc tgggtgattt ccagtggtat
    23461 cgccggcttt atctacggtt ttgctttcaa acgcacccgc gttgaagagg gtatattcgg
    23521 cggcaaagat atcctgacct acaacatcat ccaggtcatc ggcaacgcta ttgcctggat
    23581 cctggtggcg cccgtgctgg atatcgtcat ctatgctgaa cccgccaata aagtgttcgc
    23641 gcagggttta atggcttttg cctctaacgc ggtggttgcc ggtgtagtgg gcaccctgct
    23701 gctgctggct tacgccgcca cccgaaccaa aaaaggcagc ctcagcaaga aaagctgatt
    23761 tgtaatcgca tccatcacaa cagccagggt ggcaggtctt tgaccgccat cctgatgctg
    23821 attaaagtat aataactcgg tcacacggct tgtaaaaaca atcccaataa ggagcaacgc
    23881 gccggagtag agggttcagc gccggcgcct ggtaataaaa agtggtgaaa caaccaaacc
    23941 ccatcattga gtttgatcac ttttcttttc aatactattc ccaggcggaa ccaacgctgc
    24001 gggatatcaa ccttaagatc tatcccggcg aaaaaattct cattgtgggc ccgagcggca
    24061 gcggaaaaag cacgctgggg cactgcctga acgggctgat ccctttttac tacaaaggca
    24121 gcattgaagg tagcctgaaa attaacggca ttgaaacccg caacctcgat attttcaaac
    24181 tttccaagat gttgggcacc gtcctgcaag actcggacgg tcagtttgtg ggcctgacgg
    24241 tgggagaaga tatcgctttt gccatggaaa acgactgtgt gccgtatgcg gaaatgcaca
    24301 cccgcgtgga ccgtgtagcc gagatggtgg atatcgccca tgtgaagaac ctgtcgcctt
    24361 acgagctttc gggcgggcaa aaacagcgca ccgcgttggc aggggtgctc atcgacaacg
    24421 tggacatcct gcttttcgat gagccgctgg ccaacctgga ccccgccacc ggcaagaccg
    24481 ccatcgagat catcaacgat atccaccagc gaatgggcaa aaccattctc atcatcgagc
    24541 acaggctgga ggatgtgctg catcgcgctg tggaccgcgt gattgtcatc aacgaagggc
    24601 gcatcatcgc cgatatgaac gcgcatgagc tggtgagctc caatgtgctg accagcaatg
    24661 gcatccgtga gccgttgtat gtgacggcgt tgaagtacgc cggcgtccag gttaccccgg
    24721 agatgaaacc cgggtatatc tggagcctgg ataccagcgg ggtgaatgaa tcactgcggc
    24781 gctggaaccg ctcggtggtg accccgccgc ccgctccgcc aggtccggcg ttgttgacca
    24841 ttgaccactt atccttctct tatgacggtg tgcggcagac gctggaggag attaatttta
    24901 cgattactga aggagagatg gtctccattg tgggcaagaa cggtgcgggc aaatccaccc
    24961 tggccaagtt gatgtgtggt tttgaaaaag tggacaaagg agccatcctt tataagggag
    25021 agaatatccg ccataaatca attaaagagc gcgcggagtt cattggtttc gtgatgcaga
    25081 accccaacca gatgatctca aagaacctga tattcgatga gatcgcgctc gggctgcggc
    25141 tgcgcggggt ggcggaagag gaaattaaag agcgtgtgga acaggtgctg gaagtgtgcg
    25201 ggctggcgcc tttcatcgat tggccaattt ctgcgctcag ttatgggcaa aagaagcgcg
    25261 ttaccatcgc atccatcctg atactcagac ccaacatcct cattttggat gagccaacgg
    25321 ctggacagga ctaccggcat tacagcgaga ttatggagtt tttacgccag atcaacgaaa
    25381 caggcatcag catcatcctc atcacgcacg acatgcacct gatgctggag tacacaccgc
    25441 gtgccatcgt tctttctgag ggcaggctca tcaccgatgc cccggcttcg gtggtgctga
    25501 ccacgcagca agtcatcgag caagccaacc tcaaggagac ctcgctttac cggctggcgc
    25561 agctggcggg tattgaggat ggaacgcaat tcgtgcagca tttcattgac catgaaaagg
    25621 tggctgcgcg atgaaaacga agctcttctc ttacaactac gaaaacaccc ccatccataa
    25681 cctttcgggt ctgatcaagt tcatctgttt tttattgttg acctttgcca ctatgtacac
    25741 gtacgatatc cgcgtcattc tgggaatgct ggtgttctca gtgatcattt tgcgtatttc
    25801 gcgtatccgt ttcagccaga ttaaggtcat gttgatctat gtgctgatct ttatctttat
    25861 caatgccatt tttacctttt tcttttcacc cgagcacgga gtaacgcttt accacacccg
    25921 ccatcagatc gcgccgcttt gggggaacaa ggcgctcacg caagaacagt tgctttacca
    25981 ggtgaccctg gcgttcaagt atctctcagt ggtgccgtta ggtattatct ttttgctgac
    26041 gaccaacccg agcgaattcg cctcttcagt gagcggcgta ggggtgcctt acaaggcctc
    26101 tttcgccgtc tcgctgacgc tgcggtactt tccggatatt ttgcgtgtgt acaacgatat
    26161 cagccaggcg cagcaggcgc gcggactgga gatgtcggta aaagcgaaaa tgaaagagcg
    26221 cgtcaagaac tcgctgatga tcatcatccc gctcatcttt tcgacactgg aacgtattga
    26281 agtagtgacc aacgcgatgg acctgcgcgg ttttggtaag gaaaagcgcc gtacctggta
    26341 ttgcgtgcaa aagttacgcc ccgcagatta catagcactg gcggtttcga ttgtggtcac
    26401 cctcatcgta gccgcggtgg cgtacttcat taatcacggg cgttacttta atccattcat
    26461 ttgaagaaaa aatatcattt ataatgattg aataaagtta ccggcaacag gctggttcta
    26521 cccggcgggc gtgaggtcgc ctgtcaaagg agccgggcgt ttcgcctggc ggcggtgaac
    26581 aatgagcaaa ttccagtgat cggaatttac aggtaatttt tctccagcgg atactccgag
    26641 ggattgaggg gttccaaccg gatggaggtg ttcgtgaaaa aaacagtagt ggctgccgcg
    26701 ctgggggaat gcgtgcacgt ggcgggagtg gtgaactttt tacggttggc agaaaccagc
    26761 ggctggcgca cggtttttct gggtccagcg gtgggggtga aagaactgct ggcagcggcc
    26821 aaacgcgaac aggctgacct ggtgggggtc tcttaccggc tcacaccgga gacgggagaa
    26881 cggctgctgg cggaatttgc cgaggaagcc gatgagttgc gcgcgcaggg agtgaaattc
    26941 tactttggtg gcacaccgcc ggttgctgag cgtgcgcgca ggctggagtt ttttacccgc
    27001 gtgttcgatg gcagtgaaga gatagaagat gtgctggctt cgcttaaagg cgaaagggaa
    27061 gacaataccc caaaaacagg atttcccacg actgtcctcg agcgcatccg ctggaaggcg
    27121 ccataccctg ttttccgcca tcattttggg ctgccgacca tggaagccac cctggatggc
    27181 gtgagccaga ttgccgaagc cagggtgctg gatgtgattt cgctgggggt agatcaagac
    27241 gcgcaggaga attttttcca ccccgagcgg cagaacccgc gccgcacagg ggcgggaggg
    27301 gtgccggtgc gcacggtgca ggactaccag gcgctttacc aggccagccg gcgcggcaat
    27361 taccccctgt tgcgaaccta ctccggcaca gatgacttca tccgcctggc agaggtgttc
    27421 accgaaacca ttcacaacgc ctggtgcgcc atcccgcttt tctggttcaa ccagatggat
    27481 ggccgcggtc cgtgggatgt ggctggttct atccgcgaac accagcgggt gatggcgtgg
    27541 tacggcgcaa ggggcatccc ggtggaattg aacgaaccgc accactgggg aatgcgcgac
    27601 gccccggatg tggtgtgcgt ggtttcagct tacctggcag cgtacaacgc gaaagccagc
    27661 ggggtgagag attacatcgc gcaattgatg ttcaacagcc ctcctgggtt aagcgatgcc
    27721 atggacctgg cgaagatggc cgccatgctg cgcatcatcg ccccgctgga ggatgagact
    27781 tttcacattt ggcggcaaac gcgcactggc ctgttgagct acccactgca gccggaagcg
    27841 gcacgcgccc acctggcggc gagtatttat ctgcagatgt cgttgcgccc gcacatcgtg
    27901 cacattgtgg ggcacaccga ggcgcatcac gcggccaccg cgcaggatgt gatcgaggcg
    27961 gcgcgcatgg cccggcgcgc cgtggaaaac gcgctgcgcg gcgcgccgga tatgctggct
    28021 gataccgctg tgcaaaaacg cgtggcagaa ctggttaatg aagcgcaggt gaccctggca
    28081 gccatacaaa cgctggctgg cacagacgtg ccggacccac tcaccgaccc atacaccctg
    28141 gcgcaggcgt tgaaacgcgg catattagac gcgccgcagt tgcgtaacaa ccgctttgcg
    28201 cgcggcgagc tgcgcgttgc catactgaac ggcgcctgtg tggcgatcga tggtaatggc
    28261 agagtgatcg gagaagccgc gcggctcgag ggattgacca gaaaggagtg aaccgatgac
    28321 caaaacaaaa ttcactgtaa tcggtgccgg gcatggcggt aaggccatgg cggcccacct
    28381 gggattgctg ggcttcagca cccggttgta taaccggtct atggagcaca ttgcggccat
    28441 caaggagctg ggcggcatcg acctggcggg gttgaacggc gagcggcggg aatttggccg
    28501 cctggaattg gtgaccgaca acatggagca ggctttggat ggcagcgata tgatcatggt
    28561 ggtggtgcct tcatcagcgc acgctgaact ggcccgtcac atggcgccgc acctcaagga
    28621 tggacaggtc attgtgctgc atccgggccg cacctgcggc gccatcgagg tggccaaggt
    28681 gctgagagat aaccactgca cggcggatgt gaccgtttct gaagccgaga ccttcattta
    28741 cgcttcacgt tcggatgggc cggcgcaggc gcgcatcttc cgcatgaagg aagcggtgcc
    28801 gttggcggcg ctgcctgcca cgcgcaaccg cctggtgctt gattgcattc atgaagccta
    28861 tcctcaattc attgacgggg ggaatgtgct gcaaaccggg ctgaacaata tgggctccat
    28921 ttttcacccg gcgctcaccc tgctgaatgc cggttggatc gagaccaccc acggggatta
    28981 ccagttttac attgacggcg tttcgccatc agtggcgcgg gtgctggagg tgctggaccg
    29041 tgagcgagtg acggtgggtt ccgcgctggg gctgcgcacg cgcaacgcca tggaatggct
    29101 gaaactggcc tacgatacca gcggcgctga cctctacgag gctatccaca accaggaagg
    29161 ctattacggc atcaaagcgc cgcccacgct caaccaccgc tatatttttg aagatgttcc
    29221 ttgcagcctg gtgccgattg cttcgctggg agaccgtttt ggggtgagcg tgcgcgccat
    29281 ggatgccgtt gtgacgctgg ccaatatcgt gcaccgcacg gattactggc ggcgcgggcg
    29341 cacggtggat aagctgggat tcagcacctt gagcgtgagc gaactgaccg cttatgtaaa
    29401 tgagggaatt agagaagaat agaagaatga acccattcaa acttagccct tccatgctca
    29461 ctttcttctg ggaggaatgc ccgcgctgct tttacctgca ggtggtgcgc ggcatcaacc
    29521 gcccacccac tgcctttccc aaggtttttg gccggatcga cagcctgatg aagcagctgt
    29581 acgccgacca acccacctca ttcatttcag atacgttacc cgcgggcagg gtgagcctgc
    29641 agggcaagtg ggtgagctcc gagcccatcc attttgaagg tatcgcggcc tcctgttacc
    29701 tgaagggggc gtttgactcg gtgctttcct tcgatgacgg ctcgtacgcg gtgatcgatt
    29761 ttaagacctc ccgacccagc cccggtcatg ttcagttcta cgggcggcag ctgcacgcgt
    29821 acgcgtacgc gctggagcat tccgcacccg gggcgctgcg gcttagcccg gtaacacgcc
    29881 tggggttggt gtatatggac ccggtggaaa tcgattacca ccctgacgat cagcgcattt
    29941 tctatggcgg agaggtcacc tggagcgaga tcccgctggc gatggagcca tttgaagaat
    30001 ttctcgccgg ggtgttgcgc gtgctggcgg cgccagaacc cccgccagcc aacccggatt
    30061 gcggtttctg cgcctaccgc gaagaagcgc gggctcacgg aatgtgaaca tacaacccat
    30121 tcacgcgctg aacctctggt gggcaaaaaa ctggctgcca gagaaaagag gtagcgacgc
    30181 tcaatagcgc aacccgcgtt gctgacgatt acgtttcgcg cttctctttt tcttcccttc
    30241 accagcggca ggtggcttgt ggcttgctgc cgctctgcgg tgcatacaac ctgcgcgtca
    30301 atttggcgta tatgttgtag actctctaag gaggatcatg atgtttgaca ttgggcatat
    30361 actgaaacgc gcgtggggga tcctgtggaa ttaccgggta ttgtgggtct tcgctctgtt
    30421 gctggcgctc tcaggaggag cgcgtggcgg tagcggcggc ggaggagggg gtggcagctt
    30481 caacctgccg gcaaccggca gcaacttttt tgaaaacggc ggcgagggct ggctgcaacc
    30541 aggcggcgag actgcccaat ggttcaatga attggagcag tgggcggata gccacatcct
    30601 gccgttgttt gcaacggaag agacggccat tcgttcagtt atcgccatcg tcgccattct
    30661 tatgggcgtc tcgctgctca tcggcttgct gctggcgttg gtgcgctatc cttcagagac
    30721 ggcggtgatg cgcctggcgg acgcccacga gcagagcggt acaaaggtga agttcaagga
    30781 agcctggcgc ttgggttgga atgagcgcgc ctggcgcgtc tttttggtgg acctgctcat
    30841 tggcgcgccg gcgttcaccc tggtgatgat gctggtgggg ctcgtcgtct ggatggcata
    30901 taccctgatg aaaacagccg ccattcaact ggctgcgccg gcggtgattg gcatcatcct
    30961 cattgcactc ttttttgtgg cattcgcgat tttcatggtg tttgtgggct tcctgcgaca
    31021 gtacgtggcg cgttacgcgg ctattgatgg gttaggtgta ggcgcttctt tcaaacaggg
    31081 ttgggcgctc ttcaaagcgc agttcaaaca caccttcctg ctcggattgg tgcttatcgg
    31141 tgtggggctg gctttcgggt tcgccatgct gctggctgcc tttctgctca ttccggcgta
    31201 tatactgatg gcgctgcctg gggcgttggt ggcagccatc cctggcgggc tggtctattg
    31261 gatcgccagt ttctccgcgc cgccggttgt ggcggtcatc atggctgcca tcgtggccgt
    31321 gccggttttt gtaaccatca ctttcctgcc ggttagtttt ctgggcggta tgtttgctgt
    31381 cttcagcgcc aacgcctgga cgctggcttt tcgcctgctc aagccagctc acccgccgct
    31441 gccgctgaac cctggttcac cgccgcagct gccgctgtaa atcaacgaca catgtgcgag
    31501 tatggcgggg cgtagcgcgc tacgcccctt tatgctgcct gtcaaaaact gaagtgtcac
    31561 acgggcgaaa acttttcgtg ataaacttaa aaattgccat ggaac
//
LOCUS       DAOTLW010000023        29359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1865_length_29359_cov_10.585347, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000023 DAOTLW010000000
VERSION     DAOTLW010000023.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 29359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 29359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29359
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1865_length_29359_cov_10.585347"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2127)
                     /locus_tag="P5516_05190"
     CDS             complement(<1..2127)
                     /locus_tag="P5516_05190"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRT91868.1"
                     /translation="MPVDRKRMLQILNAGLATGSYRFTRQAALAWMTTYPGDLEINLL
                     YAHALVKEMRFSHAVPVIQKILRADPEYLDAAVLGEEVFAHSDPAFLPIVSGTIKALG
                     GTPIHGEKVPEWGLSLNRIRQLIQRRQNEEARQQLMEIINITEQVELACYYHLLLTKR
                     LGDRDTLITLARLYHSRWPENIQITLLLAGALLEGGETDEAVQLLHYCAANDPSAQVI
                     TRLWGDKHSFKPLYPVNMQIDFDLPIPAEVSGKLGLNQLGAGGETVPGVSPTSLTATE
                     VTSFDGYRVLPTKDEFYPEHPKVNQEPASSKKQFIAEVEATLKKVALDISQPSLSRTD
                     ERFPAYVILTMKSGLEKQYGKQSARLVIEELKNLAKTVEKNAGWTSIVFLPDDLQSCG
                     KYNITPVDRLDPWKIKLSLVDLDKALVRSGERIGAVLIVGGDEVVPFHRLPNPTDDSD
                     ESVPSDSPYGALDTNYFVTDWPVGRLPGEYGADMGLLMEQMRNLMGYHGSPATSTSLI
                     DSILNLLFFWNKGLADRHFNLGYTASVWRRSSLVAFRPIGEGKYLYLSPNGKNASFDV
                     RKLADAQVGYFNVHGVEDAGEWYGQKDPTENDSGPDFPVALRPMDIQRITRTPRIVFC
                     EACYGGHILEKAEDESIALTMLGKGVLAMVGSTTISYGSVTTPLIGADLIGYLVLKNL
                     RDGLSIGASMLKAKAEFVREMNRRQGY"
     gene            complement(2160..7475)
                     /locus_tag="P5516_05195"
     CDS             complement(2160..7475)
                     /locus_tag="P5516_05195"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT91869.1"
                     /translation="MRYLDLITTLNHLTQKQEAVVQFKALRQDPFIWEEFQQLTEDSP
                     VITSLTRQGMPLNPGTLTVLLYDPEFDFSTLNARMTSREKLEKVMFSYEEYIQGDQPP
                     ATLAQVGGLALSLLEKRKTAASWAEIIQEIVTRMKINHGDRFLLLWGTVFVVVTNLIE
                     DKDSCFRDLTIFQQAESSIEIFTHLVLCLPWTEQEKVEVLRRYLYPLSPQIQVAALKQ
                     LKKTSDGLIYAQVASKILEKYQPPDSAKKSTGDYWKNPVSSMHYAFQCQSVADIAQLA
                     GNTAVAADLNEKALEVISALLKMSKVKKADIHFDAAAESIPLGELFSTEELADPEILA
                     ELAYTSAAGDLQLSGESATAKIMQQAKKMTKAGNVDLARAELRENLKLLSESEFERLL
                     VDGPEHIQSWEPVTLLTLLVESGAYDEAERLGKVLIARNPSSEPVIAALAAAAEGKGD
                     HAAALGYLETLNALDPGSIGVMRKLADAQIRGNAYDAAYQTYRELIDQADLPEEKDLN
                     RFGEIALMVGKADEALTASAEILGRNPDSARGLTLAGMAHHRKGQDAAAIDELRRAIA
                     LAEGEVKPWIELAEIAWTGGDHSSSLTTLKEGMAANPGNAELQRVYAQRLMDEGLVSE
                     AFPYLLELSVKGHSAEVDLQLVAAMKQLGMENIDETIAGFVNRYPGDHRFIGEFGSRL
                     VWKGEQEKGLGYLKSIADHLKEDTVWSMAYLEGVLQPDYVHLVSPARLAKSELIFTKE
                     LLDDLLIREPDNLHAKMLKAEWLLQSGEYTAARKLFDAIMDESQGGRELPSARLFAGL
                     AQSAAQNGEHEIAMAALEQAISLQPSWHGLLRLKAEFLNLSGDMNEAVKQAESALEVA
                     PDHPENHVWLIEFITALGRTGNLEKHIKSAVTQYPDHLGLRLLQAQNRIGINTDEESL
                     EIEQCLLALIDKTDDAGALIKAAMIFSNLGNPEKTISCLEKAAAQGSSQAQLSLAGLY
                     RMSGELEKALSVLESVQPTTGMTELFKRETAFTHSGEGTWLDIDLDEHLRPLDIMIDE
                     VFLPMDWKELLDSSRPEIVLKSRIAMTAGSPEELMNDVRDWINAEPENLEARLYGIET
                     ALACCSQEDYQHFLDEIRLDPKHPLVGYLNLLKSERELDDGTLVRDEPVEGDIFDSVS
                     VGEPEKISFIRLLANEGNYAAAETSLEMAINVFSEVQAFPFIVKIGIFRNLAKSAAAL
                     ERWPEALQFGARATGLVPSHQVNRLMHLKHQALAQEMANRAESLGVERHCALSEQKEP
                     TPDNAEMERITNDAVKEETRRWVLRYQLAKEPTRENIRELALLTPTPEDVAALASALR
                     RIGQSRTALQVVKKFAEHPLVMFEQCVCTAENDVPKALEILERSLRIQPQQPLSLRLR
                     ADLLERTGNLTAAAESLAQALELWPNEYRWHVKFAELWQRLGNAERPVESLRLANLYA
                     QDEPEIQEKLGKALLRNEKPDEALKYLLAAVEKQPGHPDLWLSISEAHQQSGDLDLAL
                     EAAERAVQVDPYAVKARLQAGKVRWKRGELEKALDQVNLAISLNEDDASSYVLMAQLL
                     NDQGDKSKALQMLEKATQSKNADVQVLVEHASLLKDIKGASAARDLIASFSDRYPENP
                     ELLRLLAEAEDQCGNIKKAEMVAKKALAIQPEEVDLQMLLGKIQEKVGNLDQAVSYFS
                     QAIALEPRRKDAYLLLSQVYLKQRDHLKARQALEQGITNNPGELDLYLACAALLKEAK
                     DYQAAEQMLRKASSLEPRNVNIHRQLGAVLALNLVHQSQEVSSQP"
     gene            complement(7520..7831)
                     /locus_tag="P5516_05200"
     CDS             complement(7520..7831)
                     /locus_tag="P5516_05200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91870.1"
                     /translation="MTVSDFLLTMASVLLFCGVVVLGIGVFTLIGKLNGKEIRTIADQ
                     TTKLAQKGIADEISGLVGNARSLIEALDQMVKTTAGIGIFLVILGLLLIAAAYLLVTK
                     I"
     gene            complement(7903..9072)
                     /locus_tag="P5516_05205"
     CDS             complement(7903..9072)
                     /locus_tag="P5516_05205"
                     /inference="COORDINATES: protein motif:HMM:NF012385.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HRT91871.1"
                     /translation="MSLSVLVVEDEDNARSFVSTFLKKKGYEVFEASTLDEARERVKK
                     GDGDVVLLDVQLHGEYGLNLLYETAMMPARPPIIVITGFGSIDMAVDAMKNGATDFLQ
                     KPLDMSLLEKSLQRAREIVAMRRELEHYREAQMQKGNFVIGTNEVVKNTVQQALVASE
                     KQVSVLITGETGTGKDVIAKYIHSIGPRANKPFISINCAAIQSTMLESELFGHEAGAF
                     TSADRKKPGLMEIADGGVLFLNEIASMPVDIQAKLLTAIETHTFRRVGGTALIKVDVQ
                     IIAASNRNLKEMIKKGDFREDLYYRLKVVDLDMPPLRERKEDIPELVGFFIRENNARQ
                     GMNVVEVSPKAMEVMQQYSWPGNIRELSNAIERAMLFCDGMTINLADLPKDLTST"
     gene            complement(9076..11016)
                     /locus_tag="P5516_05210"
     CDS             complement(9076..11016)
                     /locus_tag="P5516_05210"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT91872.1"
                     /translation="MRAISGRFSSVLKGKNEPGLEEITALLNFDETPAALVENETRSI
                     LFINSRLMRVVSYSHRDVFRKNVGFLFPGLDLSTSFSGEIRETQIKRRDLTPLKARVR
                     FDFIDKGGKWTVIRVLPETLDEQERKLHLEDTYRLLTRFSRLADTGDIAEKIYQGVDI
                     ICEILGCETVCLYQADSSLPQLKRVAVSGNVEDFPRKLPSTDLMKLEKPTIWVTGSRI
                     MTELHREARVKNIEYVASAPMLEGGGAFGLLVAAGKGGIPVSLSLDGMEMLASMLLAL
                     FQQQVLVNNLNERIERSMYSVNLKNQVFESMNEGVIILDNLLKIEQINPAAEFMLGYA
                     AWEVKGQSYENILIGCDRLLPALVDAQKGKETLNVGKAVLNRRNGHAFPVLMKVIPVH
                     LGESVSAVEILMMDISEHEESKAVTQQLEHRAVLGDYTAAFAHDVRNPINNISTGLQL
                     LAATLDENDPSQDVINRMQGDCMRLDHLMESFLAFSRPMELKYEPFEVEPFLKRIIDR
                     WLPRMSRVNVKPILHVEEQLPKIYGDPRSLENVFTNLISNALEAMTDNGDTLAIKAVK
                     VLEVDHRPLVEISVSDNGPGIPQDIKTRIFEPFVTTRKMGTGLGLAITKQIVNAHKGS
                     IKVESFPGGTVFTVQLPADIGE"
     gene            complement(11013..12422)
                     /locus_tag="P5516_05215"
     CDS             complement(11013..12422)
                     /locus_tag="P5516_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91873.1"
                     /translation="MIQQTRQVQKPLTTAHLAQTMSLLALTAEELRQQIDSELAGNPA
                     LELVEERRCPTCHRILSDPGKCPICSIPSNPANEEPVVFLSPREEFIPRKDYVDNEVE
                     DEPFAPAAEELPTYVLRQIAPDLKKEERGLAAFMLTHLDEDGLLTTSLIEVARYTHFP
                     LSVVEKIQRAIQMADPVGVGSVSTTEALLVQLQTLRETGEVPELAEELIKDGLDDLSH
                     HQYAELAHRHHVPQKKIIAAAKFIAENLNPFPARSHWGDARQPAAQTTHVYHQPDILI
                     GFLNEDPEKPLVVEIIMPLSGTLQVNPLFKQAIRQADEDARIDLRKDLEKASLFVKCL
                     QQRNHTMERLLTRVVSLQREYIIKGDKYLKPVTRAQLSKELEVHESTISRAVANKSVQ
                     LPNKRIVPLASFFDRSLNVRSVLKEIIEKEPKTYSDSELVEMLTERGFQVARRTVAKY
                     RAIEGILPAHLRRVTIKRK"
     gene            12545..13429
                     /locus_tag="P5516_05220"
     CDS             12545..13429
                     /locus_tag="P5516_05220"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRT91874.1"
                     /translation="MRTGLIRLILVCLLLVVVGALSTSLFYKPLGREGFNAGRAYQDV
                     LFQTALGPRVPGSQAHEKVIQYFIQELKRAGWQVTLQRGERLGHSATNILASRGTLQP
                     RVIVGAHYDSRMTADREIDQSLRGTPVPGANDGASGAAVLLELARTIPTSNSEGVWLV
                     FFDLEDQGNIESWDWILGSRIFVDHLTTAPEAVIIIDMIGDRNLLIPREMNSDPELTA
                     ELWKLAAELGYENTFLDLPGYRILDDHIPFLEQGIRAVDIIDFDYPHWHTLQDTPDKV
                     SPASLEIVGNVLLAWLKR"
     gene            13501..14685
                     /locus_tag="P5516_05225"
     CDS             13501..14685
                     /locus_tag="P5516_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRT91875.1"
                     /translation="MKNSHVFMPAAQRISSFEPYFFASLGKKINQLKQQGMDIIRIDM
                     GSPDLPPADFIIEELVTQSRKSDTHGYSPTGGTQAFKQAVADYYMDRFEVALDTSSEI
                     IGLIGSKEGLFNLSQVILNPGDIALVPDPGYPVYSASGIIAGGEVVYFPLLAENGFLP
                     DLKAIPESVLQKAKILWLNYPNNPTGAVASMAFFEEVIAFARKHHILVAHDAPYVDIC
                     FDGYTAPSLLQVPGAREVAVEFNSLSKTYNMAGWRLGMAVGNAEVIDYLFTYKSQMDT
                     STFSPIFSAGVKALTGSQDWLVERNQVYKDRRDLVVNGLKQAGFTFEAPKAAIYVWAQ
                     LPAGETDSIHFCNRLLEETGISTTPGIVYGKHGEGYLRVSLGTATNRIREAMNRLVDW
                     KR"
     gene            14688..16049
                     /gene="hflX"
                     /locus_tag="P5516_05230"
     CDS             14688..16049
                     /gene="hflX"
                     /locus_tag="P5516_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HRT91876.1"
                     /translation="MAHNTIPTSPPVERAFLVGVELRSEKPLISMEDSLGELALLART
                     VNLEIVGETSQKIDVPNPSTLVGTGKVEEIALLAEECLADVVIFDTELSPRHQRELEE
                     RFGVKLRVLDRTALILDIFAQHARTSEGILQVSLAQYEYRLPRLTRAWTHLARQAGGG
                     GGRAGSIGGVGLRGPGETQLEVDRREIHKRISMLKKELEKVRAHRQRYRAQRKRSRIP
                     LISLVGYTNAGKSTLLNHLTNAGVLVADQLFATLDPTTRRIELPGGHQALITDTVGFI
                     QKLPTQLIAAFQATLEEISEADLLIHVVDVTHPNAIEQARSVEQTLREIHAEQIPVLT
                     ALNKIDRLPEPELTNEFLSGQSRSVGISALKGLGIEELLRAIEEDLFENFSQVSAQIP
                     YTEGQLISLFHEQGQVVRIEHGRKGVFIQGYIPGRLLARFFPYQSASPQDDDETESWS
                     EPN"
     gene            complement(16091..16288)
                     /gene="rpmF"
                     /locus_tag="P5516_05235"
     CDS             complement(16091..16288)
                     /gene="rpmF"
                     /locus_tag="P5516_05235"
                     /inference="COORDINATES: protein motif:HMM:TIGR01031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HRT91877.1"
                     /translation="MVPLPKRKLSKGRRDRRRAHDGLETANLVQCSNCGEMRLPHTVC
                     PNCGQYQGREVIHIEKEAKKK"
     gene            16640..17359
                     /locus_tag="P5516_05240"
     CDS             16640..17359
                     /locus_tag="P5516_05240"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRT91878.1"
                     /translation="MQIDIAVAKINKYAVSESGDTLEIVERPNGGVSVVMADGQTSGR
                     GAKTISTMVVRKVISLLADGVRDGAAARAASDSLYTERDGKVSACLNILSVDLQTATI
                     VITRNNPTPIFLARHEQVECITSDSVPIGTNRNIRPSITEFPLESGITIVMYTDGIKF
                     AGERSRQPLDICAFLESMLEDQEPTAQEIADGILNQAIRLDNNRPNDDMTVVALRSIP
                     TEVDQIRRMTVRLPVQDIQRF"
     gene            17427..17783
                     /locus_tag="P5516_05245"
     CDS             17427..17783
                     /locus_tag="P5516_05245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91879.1"
                     /translation="MIGVVGVCASGKSTLIKGLIQQGYRCRHIAQEHSYVPDMWYRLS
                     RPDILIFLQVSYEITLKRKNLNWNKSEYETQLQRLQHARQNAHIIIDTDLLTPEELVA
                     RTISEIQSLTGNSHSS"
     gene            17872..19221
                     /gene="secD"
                     /locus_tag="P5516_05250"
     CDS             17872..19221
                     /gene="secD"
                     /locus_tag="P5516_05250"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HRT91880.1"
                     /translation="MDRRYANLILILVLVAAIIWVDLPRGEGQQGLKIGNFKRTLNPV
                     LGLDLRGGMQVLLSPQKGISATRQDLEDTSKILESRANGLGVSEVIFQVAGDKYILGQ
                     FPGLTNTEDVIAAVKQTGLLEFIDSGSTQLNPGTVVETDYAAAQSGDETPAAEASGQV
                     WHTVMTGVDIKNVNVAADQGIGGYVVNFELTDSGTQVFKDHTAANVGKYLTIVLDKQV
                     ISSPRISTAIPDGKGQISGNFTSESANNLAVTLRYGRLSVPVEVVESRVVGPTLGSDS
                     LTKSLRAGVIGFIIVALFMLIYYRVPGAIAVVAIAIYAALTFALFKLIPVTLTLPSIA
                     GFLLSTGSALDANILIFERLKEELRNGRTLKQAIDLGWKRAWPSIRDSNIATIITSVI
                     LFWFGSTFGATLVKGFALTLALGVMVSLFTAVFVSRTFLGIAVDFIKTDKIKNSFGL"
     gene            19235..20155
                     /gene="secF"
                     /locus_tag="P5516_05255"
     CDS             19235..20155
                     /gene="secF"
                     /locus_tag="P5516_05255"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HRT91881.1"
                     /translation="MNILGKRYLFFALSILIILPGLIMLAVNGMPLSIDFKGGTLLEV
                     SFENGVFPSTEAIKKIYDHNGINDLQVQSTTEGTLLLRSSELSDSVRQSVLDVLAEEA
                     GSPVIILKSDTVGPSIGQGVTQRAIQAVALAALAVVIYITFAFRGVPHAFRYGVCAII
                     AMVHDVLIVLALTGYGAKFFGWQVDSLYLTALLTVIGFSVQDKIVVFDRIRENNRILR
                     KLDFEKLANHSIVQTLQRSINTQLMTVEFLLLALALFGGVSLREFAVILLVGLLSGTY
                     SSIFIAAPLLVVWENHEWKTWFTKKHATTS"
     gene            20237..21256
                     /locus_tag="P5516_05260"
     CDS             20237..21256
                     /locus_tag="P5516_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRT91882.1"
                     /translation="MRKEVVLVTGANGEIGHGLISYLGEKQASRIIALDVQPLEDNLK
                     PYCERAIQGDILDTMLLGRLVADYDIRIIFHLASILSTKAEYNPETAHRVNVEGTLNL
                     LKLAVEQSSWQGKPVKFLYPSSIAAYGLPTLEKKNQSGKVKEYDWNSPITMYGCNKLY
                     CEHLGRYYNFYYRQLAVDEVKHTIDFRCLRFPGLISSVTVPTGGTSDYGPEMLHAAAQ
                     GVPYNCFVRPDTRLPFMVMPDAIKSLLQLAEAPQENLKQLVYNVTSFSPTAEDFFHHV
                     KKAFPDAEINFKPHIARQRIVDSWPGDVEDAAAKRDWGWKPDYDEKRAFEEYLIPCIR
                     QRYAN"
     gene            complement(21253..22635)
                     /locus_tag="P5516_05265"
     CDS             complement(21253..22635)
                     /locus_tag="P5516_05265"
                     /inference="COORDINATES: protein motif:HMM:NF025042.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4147 domain-containing protein"
                     /protein_id="HRT91883.1"
                     /translation="MNIHHKPTYEERISAALNNTATPLARKGDLIKILSAALKAVDPQ
                     SVVRNNLWQIGGELRVGPNAFRLTPKSRFVTAALGKAAPAMLTGAIHQLGDYYGEGLC
                     ICKHLDSTDNPFPGTRYILGNHPVPGAGSLRAGEALKSLVIGLGEEDIVLLLLSGGGS
                     ALATLPASGITLEEMQQLTVTLLHAGADIHEINTVRKHLDLLKGGGLARLAYPARVVT
                     LVLSDVVGNNLEVIASGPSYPDPSTFADALEIVERAAKHGVIPARIRHHLERGCRGEL
                     PETLKPIEPDAQRSFNTIIASNVNASAAAVAQAKALGFTAETVTNELTGDARAAGAQI
                     ASMVKTRGDLNKPFLLVWGGETTVVVKGRGKGGRNQELALGAVKELSGVPNILLISLA
                     TDGEDGPTDAAGALVTGDTLNRALVKGLNPESYLRENDAYAFFEGIGDILRTGHTGTN
                     VNDLIFVFGF"
     gene            22743..24854
                     /locus_tag="P5516_05270"
     CDS             22743..24854
                     /locus_tag="P5516_05270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRT91884.1"
                     /translation="MDKTGIEKMLRPKSVAVIGASATPGKIGYTVIKNLLKDGYKGKI
                     YPINQKESEILGLKCYPEIGQVPGEIDSAVMCVPAKVMAEVVEECGRKGVKGALVITS
                     GFSEIGRKDLEDEVVRIARHYGMRILGPNIVGTLSNSEKFNGSFAPCLPLPGKNSLVT
                     QSGALLIAMDMATYSRRIGYEKMISIGNMADVDFADLIEWFQDDEETSCVSLYIEGLR
                     DGRRFIDVAKKSNKPIIALKSGVSAHGAAAAASHTGSLAGGAKVYSAAFKQAGVIQAT
                     DLDNLFYRGLTLSMQPPMKGDNFLIITNGGGNGVLATDAAERFGIPLKFAPDDVQAEM
                     KKHMPEYGSAKNPVDMTGMAGNDWYYETVKFAIMNKWTDSVVVLYCETAITDPAEIAA
                     SIKRAVDDSGVKDKPITVSFVGGEKSDKAIGWMVENGIPAFFAPDTAVNAMAALREYA
                     KLQENKKSDGAKTDEKAKKLALEVIAAARADGRTSLTEIEAKKVFELYGMPTTKIGLA
                     KTEEEAVKLSKEIGYPIVMKIVSPDILHKSDAGGVKVNIKTEAEAREAYNTILKNAKA
                     YKADANIHGIAVQEMAPWGTEVILGSVSDPSFGPTVMFGLGGIFVEVLKDVTFRVTPV
                     SKAEAEEMVSEIRGAPILSGVRGEAPRDRKAIIETIIKYSSMIYDLMDEIAESDANPV
                     LVYAQGEGLRVADARIILKKK"
     gene            complement(24902..25309)
                     /locus_tag="P5516_05275"
     CDS             complement(24902..25309)
                     /locus_tag="P5516_05275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91885.1"
                     /translation="MSKIYSPDSAGKERARLSKTIVLALRELMKQSEPDNLTKDITAY
                     VILALEEIAANIDTSVEAWEKRGYWVKADRFRLEWEWAGNSASRLRIAFLSEDWGAIA
                     VTTAYIGQKLNKVVVPQRHRLGTPWVGAARNLK"
     gene            25417..25498
                     /locus_tag="P5516_05280"
     tRNA            25417..25498
                     /locus_tag="P5516_05280"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:25451..25453,aa:Leu,seq:tag)
     gene            25553..25801
                     /locus_tag="P5516_05285"
     CDS             25553..25801
                     /locus_tag="P5516_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91886.1"
                     /translation="MKRENGKIAIIFILIILVILAVLLFALAPQNVKVTDPLFIVQYW
                     QNITRALANLGGFLSRFLQPIGEGIVNFFSNIGLPKIK"
     gene            26010..27524
                     /gene="tig"
                     /locus_tag="P5516_05290"
     CDS             26010..27524
                     /gene="tig"
                     /locus_tag="P5516_05290"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HRT91887.1"
                     /translation="MKLNIETREDHQAKIIAEFEPSDLDKYKIKAARRIASKSKIPGF
                     RPGKAPYDVVSRLYGEPAIEEDAIELMMEAVYPEILVEAKIDPASSGTLEDVDKGDPI
                     KFTFVVPLQPTVTLGNYKEIRKAYAPKSVTEKQVNEFIQHLRRTYATAEPVERPAAKD
                     DLVYIKLDATLLKPDKEENPELLKDRPLQLVIGENDPEENDYPYPGFGDNLIGLNAND
                     EKKIKYTYPKDSKFEKLRGKAVEFNVLLQTVKQLTLPELNDDFAKMFGEYETFAQLKE
                     AIQEQLQNRQTSEYDEMYYEELLDEVVKTATVKYPPQVLEHEMEHTIENVIHDLSHQK
                     MELDVYLKTINKEKEVWLEEEVKPAAVKNLSKSLVLRELAKAEDIELASEEIQTEVTG
                     MLTQIQQTTNPKALSKQLKNKNYLNALTMDAASRAMSRKVFERLKDIATGKTAEVAGK
                     PVKAARSKSAKEAKDSVQSTDEQKPQAKKTKAAAAKEVTPATVKKTKKVEKTSE"
     gene            27533..28138
                     /locus_tag="P5516_05295"
     CDS             27533..28138
                     /locus_tag="P5516_05295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease proteolytic subunit"
                     /protein_id="HRT91888.1"
                     /translation="MNLPDFKNVIPMVIESSGRGERAYDIYSLLFRERIIFVGTPIDD
                     QVANAIVAQLLVLSREDPDADIQMYINSPGGQIYSGLAIYDTMKMIPNKISTVAVGVT
                     ASFGTVLLAAGTKGKRYALPHATIHMHQPLGGAEGQASDIEIQAREILRLKSRLINIL
                     AESTGQKLEVIEHDTDRNFYLDAQQAVEYGLVDQVLTPPQP"
     gene            28200..28997
                     /locus_tag="P5516_05300"
     CDS             28200..28997
                     /locus_tag="P5516_05300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IIA family hydrolase"
                     /protein_id="HRT91889.1"
                     /translation="MDFEKLKSVKALIVDMDGVLWRESAPIGNLPRIFNKINALGLNY
                     ILATNNATRTPQQYVHKLAGFGVKIPVEKIINSSMAVASLLAKKHPQGGAVYIVGENG
                     LIEALANAGFYPSESNCLAVIASMDRCVSFEKLKRATLLIRSGVPFYATNPDRTYPTP
                     AGLIPGAGALVGALEIATDVHPIIAGKPNPTLYEFALERLGVTPEVTLAVGDRLETDI
                     LGGQRLGCKTALVLSGIATREEAEKFTPPVDFIFPDLESLLDCYDGN"
     gene            28984..>29359
                     /locus_tag="P5516_05305"
     CDS             28984..>29359
                     /locus_tag="P5516_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="23S rRNA m2A2503 methyltransferase; methylates the
                     C2 position of the A2530 nucleotide in 23S rRNA; may be
                     involved in antibiotic resistance; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C2)-methyltransferase"
                     /protein_id="HRT91890.1"
                     /translation="MMETDKYLFDLNQDQLKDYFVSIGEPPYRVEQLWNASYTHFARD
                     FNQVTAFPVRLRSQLASDMCFSHLKTVERFASTDGETIKNLFTLPDGNLIESVLMHYN
                     ERQTLCISSQSGCAMGCVFCATG"
BASE COUNT         7258 a         7683 c         7031 g         7387 t
ORIGIN      
        1 ataaccttgc cgtcggttca tttcccgaac aaattcggct ttcgccttca acatggaagc
       61 accgattgac agcccatcac gcagattctt caaaaccagg taaccgatca aatctgcccc
      121 aatgaggggt gtggtcacag agccatagga aatcgtcgtt gaaccaacca tcgccaaaac
      181 gcccttgccg agcatcgtca gggcaatgga ttcatcctcc gctttttcaa ggatatgccc
      241 tccgtagcag gcttcacaaa agacgatgcg aggtgtccgc gtgatccttt ggatgtccat
      301 cggtcgcaac gccaccggaa agtcgggtcc gctgtcattt tcggtgggat ccttttgccc
      361 gtaccattct ccggcatcct ccacaccatg cacattaaag taaccaacct gggcatcggc
      421 caattttcta acatcaaagc tggcattctt tccatttgga gaaaggtaaa gatacttgcc
      481 ttcaccaatg ggtctgaacg caaccagaga agaacgcctc cacacagatg cggtgtatcc
      541 taggttgaaa tgccgatctg ctaatccttt attccagaaa aataagaggt ttagaatact
      601 atcgataaga gaagttgaag tggcggggct tccatggtaa cccatcaaat tcctcatttg
      661 ttccatcagc aagcccatat cagcgccata ttcacccgga agccttccca ctggccaatc
      721 cgtcacaaag tagttggtat ccagtgcgcc ataggggctg tcagaaggca ctgactcatc
      781 tgaatcatcg gtcgggttgg gcaggcgatg aaaaggcact acttcatcac cccctacaat
      841 taaaaccgcg ccaatccttt cgccactccg gacaagtgct ttatccaggt caaccagcga
      901 gagcttaatt ttccagggat ccaacctatc gaccggggtg atattatatt taccgcagct
      961 ttgcaaatca tcaggcagga acacaatcga cgtccagccg gcgttcttct caacggtttt
     1021 cgccagattc ttcagctctt caatgaccaa tctggcgctc tgttttccat attgcttttc
     1081 caggccgctt ttcatggtga gaatcacgta cgccgggaac cgctcatccg ttcggctgag
     1141 ggatggctga gaaatatcca gggcaacctt ttttaaggtc gcctcaactt cagcaatgaa
     1201 ttgttttttt gaactggccg gttcttggtt gactttgggg tgctccggat aaaactcgtc
     1261 tttggtgggc agaaccctat acccatcaaa tgatgtaacc tctgtagccg tcaggctggt
     1321 tggcgataca ccaggaactg tttcaccacc ggcaccgagc tgatttaacc caagtttgcc
     1381 agaaacttcc gccggtatag gcaaatcaaa atcaatctgc atattgaccg ggtacagcgg
     1441 tttgaatgaa tgcttatctc cccacaacct ggtgataacc tgcgctgaag ggtcattggc
     1501 cgcgcagtaa tgcaacaact gcactgcttc atcggtttcg ccgccttcaa gcagtgcgcc
     1561 cgccagcagc aaggttatct ggatattctc aggccagcgt gagtggtaaa gccttgcgag
     1621 ggtaatcagc gtatcccggt cgccaagcct tttcgtgagc agtaaatggt agtaacaagc
     1681 aagttccact tgctctgtta tgttgatgat ctccatcaat tgttggcgtg cttcttcgtt
     1741 ctgccttcgt tggatcagct gtctgatgcg gtttaggctc aatccccact ccggaacttt
     1801 ctctccatgt attggggtac cgccgagcgc tttgatggtg cctgaaacga taggtaaaaa
     1861 ggctgggtcg ctgtgggcaa agacttcctc acccaataca gccgcatcca ggtattcggg
     1921 atctgcacgc agtatttttt gtatcaccgg tacagcgtga gaaaaccgca tctctttcac
     1981 cagcgcatgc gcgtaaagca ggttgatttc caggtctcca gggtaagtgg tcatccaggc
     2041 gagcgcagcc tgccgggtaa agcgatatga gccggtagcc aaacccgcgt tcaatatttg
     2101 cagcattcgc tttcgatcaa caggcatcgt ttttttcgct ttcgattgca tttgagggat
     2161 catggctgtg aactcacttc ctgtgattga tgcaccaggt tcaatgcaag aaccgcgccc
     2221 aattggcgat gaatattcac attacgcggt tccagtgaag aggctttgcg cagcatctgc
     2281 tcagctgcct ggtagtcctt cgcttctttc aataaagcag cgcaagcaag gtaaaggtcc
     2341 aactcacctg gattgttcgt aattccttgc tccaacgctt ggcgcgcttt taaatgatcg
     2401 cgttgcttca ggtacacctg gctgagaagc aggtacgcat cctttctcct gggttcaagc
     2461 gcaattgcct ggctgaaata gctcaccgcc tggtcaaggt tgcccacctt ctcttgaatt
     2521 tttcccagaa gcatctgtaa atcaacttct tcaggttgga tagcgagcgc ttttttggct
     2581 accatttctg ctttcttaat attgccgcat tggtcttccg cttcagccag taagcgaagt
     2641 aattctgggt tctcagggta cctatcactg aaagacgcga tgagatcgcg cgctgctgac
     2701 gcgcctttga tatcttttaa caaactggcg tgttcaacca gaacctgcac atcggcattt
     2761 ttgctttgag tggctttctc caacatttgc agcgctttgc tcttgtcacc ctgatcgttc
     2821 agtaattgcg ccatcaatac atagctgctg gcgtcatcct cgttaagtga tatcgccagg
     2881 ttcacctggt caagcgcttt ctcgagttct ccccttttcc agcgcacctt gccggcttgt
     2941 aatctggctt taaccgcgta tggatcgacc tgcaccgctc tttctgccgc ttccagcgct
     3001 aaatccaggt ctccggactg ttgatgcgct tctgaaatag acagccagag gtccgggtga
     3061 ccgggttgtt tttctaccgc cgcgagcaga tatttcagcg cttcgtcagg tttttcattc
     3121 cgcagcaagg ctttcccaag tttttcctga atctcaggct catcttgcgc atacaggttc
     3181 gccagccgaa gagactcaac aggtctttca gcattaccga gccgctgcca taactcagcg
     3241 aatttaacat gccagcggta ttcgtttggc catagttcaa gcgcctgcgc caggctttcg
     3301 gcagccgctg ttaagttgcc agtacgttca agtaaatcgg cgcgtaatct tagtgaaaga
     3361 ggttgctgcg gttggatgcg cagtgaccgc tcgagaattt caagcgcttt gggcacatca
     3421 ttttcggcag tgcagacaca ttgttcaaac atcaccagcg gatgttctgc gaatttcttg
     3481 acaacctgaa gggcagttct cgattgcccg atacgccgta aagcggatgc taacgcggct
     3541 acatcttctg gcgttggtgt cagcagagcc agctcgcgaa tattctccct ggtcggctct
     3601 ttggccagtt gataacgcag gacccaccgg cgcgtttctt ccttcaccgc gtcattggtt
     3661 attctctcca tttcggcgtt gtcaggggtc ggctccttct gttcactcaa agcacagtgc
     3721 cgttcaacac caagtgattc cgccctatta gccatttctt gcgccagcgc ctggtgtttc
     3781 aagtgcatca ggcggttgac ctggtggctc gggacgagcc ctgtcgctcg cgcgccaaat
     3841 tgtaatgcct ccggccatcg ttctaaagcc gctgccgatt tagccaggtt tcgaaaaatc
     3901 ccgattttta caataaacgg gaaagcctga acttctgaaa acacattgat agccatttca
     3961 agggaggttt cggccgccgc atagtttccc tcatttgcca acagccgaat gaatgaaatc
     4021 ttttctggtt ctccaacgct gactgaatcg aaaatgtcac cttccaccgg ctcatctcga
     4081 accagtgtac cgtcatccag ttctcgctca ctcttcagca agttaagata accaaccagt
     4141 ggatgttttg ggtccaagcg gatctcatcc aggaaatgtt gataatcctc ctgcgaacaa
     4201 caggcgagcg ccgtttcgat cccatagagg cgagcctcga ggttttctgg ttctgcgttg
     4261 atccaatccc gaacatcgtt catcaactcc tcagggcttc ccgctgtcat ggcgatgcga
     4321 gacttcaaga caatttcggg tcttgaggaa tccaacagtt ccttccagtc catcggcagg
     4381 aagacctcat caatcatgat gtccaggggc cttaagtgtt catcgaggtc tatatccaac
     4441 cacgtcccct cgcctgaatg ggtgaaggcg gtctctctct tgaaaagttc cgtcattcca
     4501 gttgttggct gtaccgattc caatacggag agggcttttt ccagttcgcc cgacatacga
     4561 tataagccgg caagagaaag ttgcgcctgc gagctcccct gggcagcggc cttttcaagg
     4621 caagagatgg tcttctcagg gttccccagg ttggaaaaaa tcatcgcggc tttgatcaac
     4681 gcgccggcat cgtccgtttt atcaatcaac gcgagcaagc attgctcgat ttccagagat
     4741 tcttcatccg tgttgatgcc aattcgattt tgcgcctgga gtaacctcag cccaaggtgg
     4801 tctgggtatt gtgtaactgc gctttttatg tgcttttcaa gatttccagt tcgccccagc
     4861 gctgttataa actcaatcaa ccagacgtgg ttctcaggat ggtcaggcgc aacttcaagc
     4921 gccgattctg cctgctttac cgcttcgttc atatcacctg ataggtttaa gaactctgct
     4981 tttagacgaa gcaatccatg ccaggaaggc tgaaggctga tcgcctgctc taacgcagcc
     5041 atcgcgattt catgttcacc attctgcgca gcggattgtg ctaatccggc aaaaagccgg
     5101 gcggaaggca gttcccgtcc accctgactt tcatccataa tcgcatcaaa taattttctc
     5161 gctgcagtat attctcctga ctggagcagc cactctgctt tcagcatttt ggcgtgtaag
     5221 ttgtcaggtt cccggataag caaatcatcc aggagctctt tggtaaatat caactcactt
     5281 ttagcgagcc tggcagggct caccaggtga acatagtccg gttgcagaac accttccaaa
     5341 tacgccatcg accagacagt atcttccttc aggtgatcgg caattgactt caaataccca
     5401 agcccttttt cctgttcgcc cttccagaca agccttgacc cgaattcacc gataaagcga
     5461 tggtcacccg ggtaccggtt gacgaaccca gcgatggttt catcgatatt ttccatgcca
     5521 agctgcttca tggcagcgac cagctgaaga tcaacttcag cagaatgccc tttcacagag
     5581 agttccagta agtacggaaa cgcttcagac accaaccctt catccatcaa tcgctgagcg
     5641 taaacgcgct gcaattccgc attacccgga ttggcagcca ttccttcttt gagcgtcgtc
     5701 aaggaggatg aatggtcacc acccgtccag gcgatttctg ccaactcgat ccagggtttg
     5761 acctcccctt cggcaagagc gatcgctctg cgcaattcat cgatcgctgc agcatcctgt
     5821 cctttacggt gatgagccat gccagccaac gtcaatcctc gagcgctgtc cgggtttctc
     5881 ccgagtattt ctgctgaagc cgtcagcgct tcatccgctt tcccaaccat taaggcgatt
     5941 tcaccaaagc ggttcaggtc tttttcttcg ggcaggtctg cctggtcaat aagttcacga
     6001 tatgtctggt aagcggcgtc ataagcgttt cccctgattt gcgcatctgc cagtttacgc
     6061 ataacgccta tcgaacctgg gtcaagggcg ttcagtgttt caagataccc cagcgcggca
     6121 gcatggtctc cttttccttc cgccgcagcc gctaacgccg caataacagg ttcactcgag
     6181 gggttacgcg ctattaatac tttaccgagg cgctctgctt catcgtaagc gccgctttct
     6241 acaagcaaag tcagcagtgt gacgggctcc caactttgaa tgtgttccgg gccatcgaca
     6301 agcagccgtt cgaattcgct ttcagaaagc agttttaggt tttctcgcaa ttctgcccgg
     6361 gcgagatcaa cgttacccgc tttcgtcatt ttctttgctt gctgcataat cttcgcggtc
     6421 gcggattcac cgcttagctg taaatcaccg gctgcgcttg tgtacgcgag ctctgcgagt
     6481 atttctggat cagccagttc ttcggttgaa aaaagttcac caagcggaat gctctcagcg
     6541 gctgcatcga aatggatatc ggccttcttt accttgctca ttttgagcaa cgctgaaata
     6601 acctcgagcg ctttttcatt aaggtccgcg gcaacagccg tgttgccagc cagctgtgcg
     6661 atatccgcaa ccgattggca ttgaaaggcg taatgcatag acgaaaccgg gtttttccag
     6721 taatctccgg tggacttttt tgcgctgtct ggtggttggt atttttccag gattttggag
     6781 gcaacctgcg cgtaaatgag accgtcactg gttttcttca actgtttcaa tgccgctacc
     6841 tgtatctgcg ggcttagggg atagaggtag cggcgcagga cctccacttt ttcctgctcg
     6901 gtccagggca ggcacaaaac gagatgggtg aagatttcta tactgctttc cgcctgctga
     6961 aaaatcgtga ggtcgcgaaa acatgagtct ttatcttcaa tcaaattcgt caccaccacg
     7021 aagacagtcc cccataagag gagaaaccta tctccgtggt taattttcat cctggtcacg
     7081 atctcttgaa taatttctgc ccaactcgcg gccgttttcc gtttttccag caaagacaac
     7141 gccagccccc cgacctgcgc cagggtcgcc ggtggttgat caccctggat gtactcttcg
     7201 taactgaaca tgaccttttc taacttttct cgggaggtca ttctggcgtt caaagtggaa
     7261 aaatcgaact cagggtcata aaggaggact gtcaaagtac ctgggttgag cggcattccc
     7321 tgccgggtta aagaggttat aaccggagaa tcttcagtga gttgttgaaa ttcttcccag
     7381 atgaaaggat cctggcgcaa tgctttaaac tgaacgacag cctcttgttt ttgcgtgagg
     7441 tggttcagcg tggttatgag atcgagataa cgcatttcgt ctcctctcct ggctatcaga
     7501 gttggattac ccgcagactt tatattttcg ttaccagcaa ataggcggcc gctatcagca
     7561 acaaccccag aattaccagg aaaatcccga ttcccgcggt ggtctttacc atctggtcaa
     7621 gcgcttcaat cagagaacgc gcattgccca ccaggcccga aatttcatcg gcaatgccct
     7681 tttgcgcgag tttggtggtt tggtctgcaa tggtgcgaat ttccttccca ttgagcttgc
     7741 caatcaaagt aaaaacgcca atccccagta ccaccacgcc gcaaaataac aacacgctcg
     7801 ccatggtcaa caggaagtca cttacagtca ttgaaaacat aaagccctcc aaaatagttg
     7861 ctctggaggg tttgatgcaa gtattatgcc atcattattt gcttacgttg aggtcaggtc
     7921 cttaggaagg tccgccaggt tgatggtcat tccatcacag aaaagcatag ccctttcaat
     7981 cgcgttagat agctccctga tgttgcccgg ccaggaatac tgctgcatta cctccatggc
     8041 tttcggcgaa acctcaacca cattcatacc ctgcctggcg ttattttcgc ggataaagaa
     8101 tcccaccagt tcaggaatat cctctttccg ttctctgagc ggaggcatat ccaggtccac
     8161 caccttcaac cggtaataca ggtcttctcg gaagtcgcct tttttaatca tctctttcaa
     8221 gtttcgattt gacgcggcga ttatctgcac atccacctta atcaaagcag tgccgcctac
     8281 ccggcgaaag gtgtgagttt cgattgcggt cagcaatttt gcctgaatat ccaccggcat
     8341 tgaagcgatt tcgttgagga ataaaacgcc gccatctgcg atttccatca aacccggttt
     8401 ttttcggtca gcgctggtga aagcccccgc ttcgtggcca aaaagttccg attcaagcat
     8461 tgtgctttga atggctgcac aattgataga gataaaaggc ttgttcgctc gtggcccaat
     8521 agaatgaatg tacttggcga tgacatcttt tcctgttccg gtttccccgg tgatgagcac
     8581 ggaaacctgt ttttcagacg ccaccagtgc ctgttgaacg gtatttttca caacttcatt
     8641 cgtgccgatc acgaaattcc ctttttgcat ctgcgcttca cgatagtgtt ctaactcacg
     8701 gcgcatggcc actatctcac gagcgcgttg caacgacttt tccaatagcg acatatccaa
     8761 aggtttttga agaaaatcag tggcgccatt cttcatcgcg tcaaccgcca tatcgatact
     8821 gccaaagccg gtaatcacga tgattggtgg gcgcgcgggc atcatcgctg tttcatacag
     8881 caggtttaaa ccatattctc catggagctg cacatccagc agcactacat cgccatcccc
     8941 tttcttcacc ctttcacgag cctcatccag tgtactcgct tcgaaaacct catacccctt
     9001 ctttttgaga aaggtgctaa cgaagctcct cgcattatct tcatcttcca caaccaaaac
     9061 agataaactc ataggtcatt ctccgatgtc cgcaggcaat tgtacagtga atactgttcc
     9121 tccgggaaaa gattctacct tgatactgcc tttatgggcg tttacgattt gcttggtgat
     9181 cgccagcccc agccctgtac ccatcttcct ggtggtcaca aagggctcaa aaatacgcgt
     9241 tttgatatcc tgtggaatcc ccggcccatt gtctgaaaca ctgatctcta caagcgggcg
     9301 atggtctact tccagaacct ttactgcctt aatcgccagc gtgtcgccgt tgtctgtcat
     9361 cgcttcaagc gcgttggaaa tcagattagt gaaaacgttt tcaagcgagc gtggatctcc
     9421 ataaattttg ggcaattgtt cttccacgtg cagtatgggt ttcacgttca ccctcgacat
     9481 gcgtggcagc caacgatcaa tgatgcgctt cagaaacggt tcaacttcaa atggctcgta
     9541 cttcagttcc atcgggcgtg aaaacgctaa aaacgactcc atcaggtggt caagccgcat
     9601 gcaatctccc tgcatgcggt ttatcacatc ctggctgggg tcattttcat ccaatgttgc
     9661 agcaagcagc tgcagacccg tggatatgtt attgatgggg tttctcacgt catgggcaaa
     9721 agccgctgtg taatcaccca acacagcgcg atgttcgagc tgctgcgtaa ccgccttgct
     9781 ttcctcgtgt tcgctgatat ccatcatcaa tatttccaca gccgatacgc tctcacccag
     9841 gtgtactgga attactttca tcagaactgg aaatgcgtgc ccattcctgc ggttaagcac
     9901 ggccttgccc acgttgagcg tttcctttcc tttttgggca tccacgagcg cgggtaacag
     9961 gcggtcacaa ccgatcagaa tgttttcata actttgccct tttacctccc aggctgcgta
    10021 ccccaacata aactcagccg ccgggttaat ttgctcaatt ttcagcagat tatcgagtat
    10081 gatcacacct tcattcatcg actcaaaaac ctggttcttc aagttaaccg agtacatgga
    10141 acgctcaatt ctttcattga gattattcac cagcacttgc tgttgaaaaa gagcaagcag
    10201 cattgaagcg agcatttcca tcccgtccag gctcaagctt acgggaatac cacccttgcc
    10261 cgccgcgacg agcagaccaa aagccccccc accctccagc atcggcgcgg aggcaacata
    10321 ctcaatattc ttcaccctgg cttcgcggtg cagttcagtc ataattcgag aaccggtcac
    10381 ccagatggtt ggcttttcca acttcatcaa gtcggtggat ggaagcttac gaggaaaatc
    10441 ttccacattc cccgaaaccg ccactctttt caattgcggg agagaagagt ccgcttgata
    10501 aaggcatacc gtctcacaac ccaatatttc acagataata tccacgccct ggtagatttt
    10561 ttcggcgata tctccggtgt ctgctaagcg ggaaaagcgg gtgagcaacc gatacgtatc
    10621 ttcgagatgg agtttcctct cttgttcgtc cagtgtttct ggcagaaccc gaatgacggt
    10681 ccatttccca cccttgtcga tgaagtcaaa acgaacgcgc gccttcaaag gagtgaggtc
    10741 tctccgtttg atttgcgttt ccctgatctc tccagaaaaa gacgtggaca aatccaatcc
    10801 ggggaacaga aaaccaacat ttttacgaaa aacatcgcga tgagaataag aaaccaccct
    10861 catcaaccgg ctgttaatga acaggatgct tctcgtttcg ttctctacca aagcggcagg
    10921 ggtttcatca aaatttaata gtgcagtgat ctcctcgaga ccgggttcat ttttcccttt
    10981 tagcacggac gagaacctgc ctgaaatcgc cctcattttc ttttaatcgt gacccttcgt
    11041 aaatgcgccg gtaaaatgcc ctcaatcgct cgatacttgg caaccgttcg cctcgccact
    11101 tgaaaacctc tttcagtcaa catttcaacc aattcagaat cactgtacgt tttgggttct
    11161 ttctcgataa tttccttgag aacactgcgc acgttcaggc tgcggtcaaa gaaagaagcc
    11221 agcggaacga tccttttatt gggtaattgc accgacttat tcgcgacagc gcgtgagata
    11281 gtagattcgt gcacttcgag ttccttcgaa agctgcgcac gcgtcaccgg cttgagatac
    11341 ttatcccctt ttataatgta ttcgcgctgc aagcttacca cacgggtgag caaccgttcc
    11401 atggtgtggt tgcgttgttg aaggcacttc acaaataatg aagctttctc caggtccttg
    11461 cgtaaatcaa tcctggcatc ttcatctgcc tggcgtatcg cctgtttaaa caacgggtta
    11521 acttgcagcg ttccacttag gggcatgatg atttctacca ccagcggttt ttcaggatct
    11581 tcatttagga aaccaatcag gatatccggc tgatgataca catgcgtcgt ttgagcagcc
    11641 ggttggcggg catctcccca gtgcgagcgt gctggaaaag gattaaggtt ttcggcgatg
    11701 aatttcgcgg cggcaataat tttcttttgc ggtacatgat gacgatgcgc gagttcagcg
    11761 tattgatgat gactgagatc gtccagcccg tctttgatca actcttcggc cagttccgga
    11821 acctcacccg tttcacgtaa agtttgcaat tggaccagta acgcttctgt cgtggagacc
    11881 gatccaactc ctacggggtc tgccatttgg atggcacgct gaattttttc gaccacagat
    11941 aacgggaaat gagtgtaccg ggcaacctcg atcagactgg tggtcagcag cccatcttca
    12001 tcaagatggg tcagcatgaa ggcagctaat ccgcgctcct ctttctttaa atctggagcg
    12061 atttgtcgca gtacataagt cggcaactcc tccgcggctg gggcaaaagg ctcatcctcc
    12121 acttcattat ccacataatc tttcctgggg ataaactctt cgcgcggtga aagaaatacc
    12181 accggttctt cgtttgccgg gttgctcggg atgctgcaaa tagggcattt acctgggtca
    12241 gacaagatgc ggtggcaggt cgggcagcgc ctttcttcaa ctaattccag agccgggttt
    12301 cctgccagct cagaatcaat ttgctgccgt agttcctccg cggtaagcgc aagcagcgac
    12361 attgtttggg caaggtgcgc agttgtgagc ggtttttgaa cctggcgggt ttgctgtatc
    12421 atagtattca aatcctaatg aagtatagcc tttttaaagc aatcgtgcaa gttctatgcc
    12481 agcgtttttc ggttaaaatg gtcgtgaaat catcgctaaa gcgatgatta ataaaggcgc
    12541 aataatgaga actggcctaa tccgtttgat actggtttgt ttgctcctcg tggttgttgg
    12601 agctctgagc acttctcttt tctataaacc tttaggcaga gagggtttca acgccggacg
    12661 cgcgtatcaa gatgtcctct ttcaaacagc gcttggtcct cgcgtgcctg gttctcaggc
    12721 gcacgaaaaa gttatccaat atttcattca ggagttaaaa cgagccgggt ggcaggtgac
    12781 gcttcagcgt ggtgaaaggt tggggcactc agccaccaat atccttgcaa gcaggggcac
    12841 cctgcagccg cgggtgatcg ttggagcgca ttacgacagc cggatgacag ccgaccggga
    12901 gattgaccaa agcctacgag gtacgcccgt tcctggcgca aacgatggcg catcaggcgc
    12961 agcggtgctg cttgaactgg ccagaacaat cccaacttct aacagtgagg gagtctggtt
    13021 ggttttcttt gatttagaag accagggaaa tattgagagt tgggactgga ttctgggttc
    13081 gcgtattttt gttgaccatc taacaacggc tccagaagca gtgatcatca tcgatatgat
    13141 tggcgatcga aatttgctca ttcctcgaga aatgaactca gaccccgaac tgaccgcaga
    13201 gctctggaag ctggctgcgg aattaggtta tgaaaatact tttcttgact tgcccgggta
    13261 caggattctt gatgatcaca ttccttttct tgaacagggt attcgagcgg tcgacatcat
    13321 cgattttgat tatccccact ggcacaccct ccaggacact cccgataagg tctctccagc
    13381 cagccttgaa atagtgggca atgtactgct ggcctggctg aagcgttaag tgattgtcaa
    13441 ttgaccgtaa atgacttaca ctactgatac tattaaaaca accgaaggaa aacaacaact
    13501 atgaaaaata gccatgtctt catgcctgct gcccaacgga tttcatcgtt tgagccctac
    13561 ttttttgctt ctttggggaa aaagataaac caattgaaac agcagggtat ggatattatc
    13621 cgaattgaca tggggtcgcc tgacctccca cccgctgatt ttattatcga agagttggtg
    13681 acacaaagtc gaaaatctga tacgcatggt tattctccta ctggagggac acaggctttc
    13741 aaacaggcgg tggctgacta ttacatggat cgttttgagg ttgcgctgga cacttccagt
    13801 gaaattatcg gcttgatcgg ctcaaaagaa gggctattta acctcagcca ggttatcctc
    13861 aaccctggag atatcgcatt ggtacctgac cctggctacc ccgtttacag cgccagcggt
    13921 atcatcgctg gcggcgaggt ggtctatttc cccttattag ctgagaatgg cttcctgccc
    13981 gacctaaagg cgattcctga aagtgtgctg caaaaagcca aaatcctctg gctgaactac
    14041 cccaataacc ctacaggcgc tgtcgccagt atggcgtttt ttgaggaagt catcgctttc
    14101 gcccgcaagc accacatcct cgtggctcat gacgccccat acgtggatat ttgcttcgat
    14161 ggttacacag cccccagcct cttgcaggta ccaggcgcca gagaggtagc cgttgaattt
    14221 aactcacttt caaaaacata taacatggca gggtggcgtt tgggtatggc agtgggcaac
    14281 gcggaagtga tcgattatct cttcacttat aaaagccaga tggatacatc cacattctct
    14341 cccatttttt ccgcaggggt caaagcgttg accggaagcc aggattggtt ggttgagcgc
    14401 aatcaggtct acaaagaccg gcgggatctg gttgtgaacg gattgaagca ggcgggcttc
    14461 acctttgaag ctcctaaggc cgccatttac gtgtgggcgc aactccctgc cggcgagaca
    14521 gattccatcc atttttgtaa ccggttgtta gaagaaaccg gcatcagtac cacgcctggt
    14581 attgtctacg gaaaacatgg tgaaggctat ttgcgggttt ctttgggtac cgcaaccaat
    14641 cgcatccgcg aagcgatgaa ccgcctggtt gattggaaaa gataagcatg gctcacaata
    14701 ccatacccac cagccctcct gttgagcggg cttttctcgt gggagtggaa cttcgtagtg
    14761 aaaaaccgct gatctcaatg gaagattcac tgggggaact cgccttgctg gccagaacgg
    14821 tgaacctgga gatagtaggc gagacctctc aaaaaatcga tgttcccaat cccagcacgc
    14881 tggttggaac tggaaaggtc gaggaaattg ccttattggc ggaagaatgc ctggcagatg
    14941 tggtcatctt cgatactgaa ctttctccca ggcatcagcg cgagctggaa gagaggtttg
    15001 gtgttaaatt acgcgtactt gaccgcacgg cattaattct ggatattttt gcccagcacg
    15061 cgcgcacaag tgaaggtatc ttgcaggtat cgttagcgca atacgagtac cgcctgccca
    15121 ggctgacccg cgcctggacc cacctggcaa ggcaggcggg cggcggcggt gggcgcgctg
    15181 gttcgattgg cggcgtcggc ttacgtgggc ccggagaaac ccagttagag gtggaccggc
    15241 gggaaattca caaacgcatt tcaatgctca agaaggaact tgaaaaagtc cgcgctcacc
    15301 gccagcgtta ccgcgcgcag cgaaagcgtt cacgcatccc tctaatcagc ctggttggat
    15361 acaccaatgc tggtaaatcc acccttctaa accacctcac caacgctggc gtgttggtcg
    15421 cagaccaact ttttgccacc cttgacccga caacgcgaag aattgaactc cccggcgggc
    15481 accaggcgct gattactgac acggtcggct tcatccaaaa actgccgact cagttgatcg
    15541 cagccttcca ggcaaccctt gaagaaataa gcgaagcgga cctgttaatc cacgtggtgg
    15601 atgtgacgca tcccaatgcc attgaacagg ccaggtcagt ggaacagaca ctgcgcgaga
    15661 tccacgccga gcaaatccca gtgcttaccg cccttaataa gatagaccgg ctgccagagc
    15721 ctgaacttac caatgaattc ctatccggtc aatcacgatc ggtgggaatt tccgcattaa
    15781 agggattggg aattgaagaa ttactgagag cgattgagga agatttgttt gagaatttca
    15841 gccaggtttc cgctcagatt ccctatacgg aaggacaatt aatttctctg tttcacgagc
    15901 aaggtcaggt ggtgcgtatt gaacatggac gtaaaggcgt ctttattcaa ggatatatcc
    15961 ccgggcggtt gctggcgcga ttcttcccat accagtcagc gtcaccacaa gacgatgatg
    16021 aaactgaatc atggtcagaa ccgaattaaa tcaaaaacgg gcttgtgtcg cccgtcattg
    16081 atgtttttaa tcactttttc ttggcttctt tttcaatatg gatgacttct cttccttgat
    16141 actgcccaca gttggggcag accgtatgcg ggagtctcat ctctccacaa ttgctgcatt
    16201 gaacaagatt cgcggtctcg agcccatcat gcgcgcgacg gcggtctctt ctacccttag
    16261 aaagctttct ttttggaagc ggaaccattt ctcttctcct gtcaaaaatt cctcacccgt
    16321 catgttttga caggccgggt gattatacat aacttgcccg ttgccgtcaa cctcacctca
    16381 ccgtgttagt tgttatacca aagttaaaat gtcccctttt accaaagtta aaatgtcccc
    16441 gtatgtggag gctgaaagtg gacagaatct acaacatgag tgccaaagag atacgcagga
    16501 cagaaatcat gagtcagatg gcgaggaaac agattacgca aatagaggag gccaaacggc
    16561 tggggataag cgtgcagcaa ggcgcatcag gtaatcaacg gaaaaacaaa gaaacagtat
    16621 tttgatgtaa aatctatgca tgcaaatcga tatcgcggtg gcaaaaatca acaaatatgc
    16681 cgtatctgaa agcggtgata cgctggaaat tgtggaacga ccaaatgggg gcgtatcagt
    16741 ggtgatggca gatgggcaga cttccggtcg cggcgcgaaa accatctcca ccatggtcgt
    16801 gcgtaaggtg atcagcctgc tggcagacgg tgtgcgagat ggagccgctg cccgcgccgc
    16861 ttctgattcg ttgtacacag agcgggatgg gaaagtatcc gcctgtttga acattctttc
    16921 tgttgacctc caaaccgcca ccatcgtcat tacccgcaac aatcccaccc ccattttcct
    16981 ggcaagacac gagcaagtgg agtgcattac ctctgatagt gttcccattg gtaccaaccg
    17041 caacatccgc ccttccataa ccgagttccc cctggaaagc ggcattacca tcgtgatgta
    17101 tacagatgga atcaaattcg ccggggaacg ctctcggcag ccattggata tctgcgcctt
    17161 ccttgaatcc atgttggaag accaggagcc aaccgcccag gaaattgccg atggcatcct
    17221 gaaccaggcc atcaggctgg ataataatcg gccgaacgat gatatgaccg tggtcgccct
    17281 gcgctcgatc cctactgagg ttgaccagat aaggcgtatg accgtcagac tgcccgtcca
    17341 agacatccag aggttttaac cgagttttca aagttcagga gaactcctcc tcctttccaa
    17401 ccatgagctc tataagaact gaccttttga taggggttgt aggcgtttgc gcctccggta
    17461 aatccacctt gatcaaaggg ttaattcaac aagggtatcg atgcaggcac atcgctcaag
    17521 agcattctta tgtaccagac atgtggtacc gcctttccag gccggatatt ctcatctttt
    17581 tacaggtatc ctacgaaatc acacttaaac gcaagaatct taactggaac aagagcgaat
    17641 acgaaaccca gctgcaaagg cttcagcacg ccaggcagaa tgctcacatc attatcgata
    17701 ctgacctcct gacgccggaa gagcttgttg cgagaacgat aagtgagatc cagtctctga
    17761 cagggaattc tcattccagt taagtcaact tttataatgg tttgaggtat aatccctgag
    17821 ttcccgcctt ttttcggcag gattttaatc gcattggagg tagaacatca catggatcgt
    17881 cgttatgcaa atctgatcct gatcctcgtc ctcgtcgcag ccattatttg ggtggacctg
    17941 ccccgtggtg aaggccaaca gggcttaaag attggtaatt tcaagcgcac acttaaccct
    18001 gtgctcgggc tggacctgcg cggtggaatg caggtgctcc tcagccccca aaaagggatc
    18061 tctgccacac gccaggacct tgaagacaca agcaagatcc tggaaagccg cgccaacggg
    18121 cttggtgtga gcgaggtgat cttccaggtc gcaggagata agtatattct tgggcaattc
    18181 cccggattga ccaatacaga agatgtgatt gccgcggtta agcaaactgg tttactcgaa
    18241 tttatcgatt caggtagcac gcagttaaac cctggcacag tggtagaaac ggattacgct
    18301 gctgcccagt ctggcgatga aactccagca gccgaagcgt ccggacaggt ctggcatacg
    18361 gtaatgaccg gcgtggatat taaaaacgta aacgtcgctg cagaccaggg gattggcggg
    18421 tatgttgtca atttcgaact cactgatagc ggcacacagg tatttaaaga ccataccgct
    18481 gccaatgtcg gtaagtacct gaccatcgtg ctcgacaaac aggtaatctc cagcccgcga
    18541 atcagcaccg ccattccaga tggaaagggg caaatttcgg gcaatttcac aagcgaatca
    18601 gccaataacc tggcagtaac cctgcgctat gggcgccttt cagtgcctgt tgaagtagta
    18661 gaaagccgtg tcgtcggccc aacccttggg tcagactcac taacgaaaag ccttagagcg
    18721 ggcgtcatcg gctttatcat tgtagccctg ttcatgttaa tttattatcg tgttccaggg
    18781 gctatcgctg ttgtcgctat cgctatttac gcagcgctca cgttcgcctt atttaaactg
    18841 attcctgtca ccctcacgct tcccagcatc gcaggctttc tcttatccac cggcagtgcg
    18901 ctggacgcca acattctgat tttcgaaaga cttaaggaag aactccgtaa cgggcgcacc
    18961 ctgaaacagg ccattgatct gggatggaag cgcgcctggc cttccatacg agattcgaac
    19021 atcgccacga tcatcaccag cgtcatcctt ttctggttcg gatcaacatt tggcgctaca
    19081 ctggtgaaag gctttgccct cactctggcg ctgggagtga tggtttcatt gttcaccgcc
    19141 gtgtttgtca gccgaacctt cctcggaatc gcggtcgatt tcataaaaac cgataagatc
    19201 aagaattcat tcggtttgta gaaggaaaat aataatgaac atacttggta aaagatatct
    19261 cttttttgca ctctccatcc tcattattct gccagggttg atcatgctcg cggtgaatgg
    19321 aatgccttta tcgattgatt tcaaaggcgg cacattgctt gaagtgagtt ttgagaacgg
    19381 cgttttccct tccaccgaag cgataaaaaa gatctatgac cacaatggca ttaatgacct
    19441 tcaggttcaa tcgaccaccg aaggcacttt gcttttacgc tcatcagaat taagtgatag
    19501 tgtgcgccaa tccgttctgg atgtcttggc agaagaagcc ggcagcccgg tgattattct
    19561 aaaatcagac acagtcggcc cttctatcgg tcaaggggtc acacagcggg caatccaggc
    19621 ggtcgcgctt gcagctttgg cggtggtaat ttacatcacc ttcgcctttc gcggggtacc
    19681 gcacgctttt cgttatggcg tgtgcgccat tatcgccatg gtccacgatg tgctcatcgt
    19741 tctcgccttg accggttacg gagccaagtt cttcggttgg caagtcgatt ctttatacct
    19801 taccgctcta cttacagtca tcggtttttc agtccaggat aagatcgtgg tgttcgaccg
    19861 catccgcgag aacaaccgta tccttcgcaa gttagacttc gagaagctgg cgaaccactc
    19921 cattgtgcag acgctgcagc gttccattaa cacccagctc atgacggttg aattcttgtt
    19981 gctcgctttg gcgctttttg gcggcgtcag cctgcgggag tttgctgtga tcctcctggt
    20041 tggtttatta agcggaactt actcctccat cttcatcgcg gctccgctcc tggtggtttg
    20101 ggaaaaccat gagtggaaga cctggttcac aaagaaacac gcaacgactt cgtaaatcaa
    20161 aagagttctg gagcaatcca gaactttttt acactaggca agtaagcagt caacctactg
    20221 aacaggaggg atgcgcatgc gaaaagaagt ggtcttggtc actggcgcga atggagagat
    20281 aggtcatgga ttgatcagct atctgggtga aaaacaagca agccggatca tcgcccttga
    20341 tgttcagccc ttggaagaca atctgaaacc ttattgcgag cgggcgatcc agggtgacat
    20401 tcttgatacc atgcttctgg gccgactggt ggctgattat gatatccgca taatctttca
    20461 tctggcgtcc attctttcca caaaagcaga atacaaccct gaaaccgctc accgggttaa
    20521 tgttgaagga acgctgaacc tgctcaaatt ggcggttgaa caatcttcct ggcagggaaa
    20581 accggtcaag ttcctttatc ccagttcaat tgccgcatat gggttaccca cactcgaaaa
    20641 gaaaaatcag tctggcaagg ttaaagaata cgattggaac tcacccatca cgatgtacgg
    20701 ctgcaacaag ctttactgtg aacaccttgg caggtattac aacttttact atcggcaact
    20761 ggcagtggat gaagtaaaac atactattga ctttcgttgc ctgcgttttc cggggttaat
    20821 ttcatcggtc actgttccaa cagggggaac cagtgactac ggacctgaaa tgttacacgc
    20881 tgcagcccag ggcgtaccct ataactgctt cgtccgtcct gatacgcgct taccttttat
    20941 ggtaatgcct gatgccatca aatccttgct gcagcttgcc gaggcgccgc aagagaattt
    21001 aaaacagttg gtttataacg tcaccagctt cagccctacc gcagaggatt tcttccatca
    21061 cgtgaagaag gcattccctg acgctgagat aaattttaaa ccgcatattg cgcggcagcg
    21121 aatcgtggat tcctggccag gtgacgtgga agacgccgcg gccaagcgag attggggatg
    21181 gaaacctgat tacgacgaaa aacgtgcctt tgaagaatac cttattccct gcatccggca
    21241 aaggtacgcg aattaaaaac caaaaacgaa aatcaaatcg ttgacattcg tgccggtatg
    21301 cccggtgcga aggatgtctc cgattccctc aaagaatgcg taagcatcgt tttcgcgcag
    21361 gtagctttca gggttaaggc cttttaccaa tgcgcggttc agggtatctc ctgtcaccaa
    21421 cgccccggca gcgtctgtag ggccatcttc tccatctgtt gccaggctga tgagcaggat
    21481 gttcggaact ccagataatt ctttcaccgc tcccaacgcc agctcttgat tcctgccgcc
    21541 ctttccacgt cctttcacca ccacggtcgt ttctccgccc cataccagca gaaagggttt
    21601 gttcaagtcg cccctggtct tcaccatcga agcgatctgc gcccctgctg ctcgcgcatc
    21661 gcccgtcaac tcgtttgtga cagtctcagc tgtaaaaccg agcgcctttg cctgtgctac
    21721 ggcagcagcg ctggcattca cattgctggc aatgatcgta ttaaaacttc gctgggcatc
    21781 aggttcgatg ggtttcaacg tttcaggtaa ttctcccctg cagcctcttt ctaaatggtg
    21841 gcgaatacga gcgggaatca cgccgtgttt cgctgcacgt tcaacgatct ccagagcgtc
    21901 cgcaaaagta gaaggatcag ggtaggacgg tccagaggcg ataacctcca ggttgttgcc
    21961 cacaacatct gacagcacca gtgtcacaac cctggctgga tacgccagcc gcgccagtcc
    22021 gcctcctttg agaaggtcga gatgcttgcg tacggtattg atttcatgaa tatccgcgcc
    22081 cgcatgtaag agggtcactg ttaactgctg catttcctca agggttatgc cgctggctgg
    22141 caatgtagcc agggcagacc caccgccgga aagcagcagc agcacgatat cctcttcacc
    22201 cagaccaatg accaggcttt ttaatgcttc tccagcccgc aggctgcccg cgcccggtac
    22261 tggatgattt ccaaggatat aacgggtacc agggaacggg ttgtctgtag aatcaaggtg
    22321 tttacagatg cataatccct ctccataata gtcgccaagc tggtggatgg cgccggtcag
    22381 catcgccggt gccgctttcc ccagtgctgc ggtcacgaac cttgattttg gggtcagccg
    22441 aaaagcgttt ggacccacgc gtaattcacc cccgatttgc cacaaattat ttctcactac
    22501 cgactgcgga tcaactgcct tcagtgctgc cgagagaatc ttgatcaaat caccttttct
    22561 tgcgagtggc gtggcggtgt tgttcagcgc agcggagatt ctttcttcat aggttggttt
    22621 atgatgaata ttcatagatt tgaggacttt tgttttgcga aaagtgggta aaatgacagt
    22681 tgaattattc tatcatttgt tcaagacaac ctgattgatc aatccaaaag gaggaatttg
    22741 ctgtggataa aactggaatt gaaaagatgc tcagaccgaa atctgtcgcg gtgattggcg
    22801 cttcggctac accggggaag atcgggtaca ctgtcatcaa gaacctgctg aaagacggtt
    22861 acaagggcaa aatttaccct atcaaccaga aagaaagcga gattctcggt ttgaagtgct
    22921 acccggaaat tgggcaggtt ccgggcgaga tcgactctgc tgtcatgtgc gttccagcca
    22981 aagttatggc ggaagtagtt gaagaatgcg gccgtaaagg cgtaaaaggc gccctggtaa
    23041 tcacttcagg gtttagcgag attggccgaa aagacctgga agatgaagtg gtcagaatcg
    23101 ctcgccacta tggtatgcgc atcctcggcc ccaacatcgt tggcacgctc tccaattccg
    23161 aaaagtttaa tgggtcattc gcgccctgcc ttcctttgcc gggcaagaac tcactggtca
    23221 cccaatcagg cgcgttgtta attgccatgg acatggcaac ttacagccgc cggattggat
    23281 acgaaaaaat gatatctatc ggcaacatgg ccgatgttga ttttgccgac ctgatcgaat
    23341 ggttccagga tgatgaggaa acctcctgcg tttctctcta catagaaggt cttcgggacg
    23401 ggcgcaggtt tatcgatgtg gccaaaaaat ccaacaagcc catcatcgcg ctcaagtctg
    23461 gcgtttctgc tcacggcgcg gcggctgcag cctctcacac tggctcgctc gccggaggcg
    23521 caaaggtgta cagcgcagct tttaaacagg caggtgtgat tcaggcaacg gatcttgata
    23581 acctcttcta ccgcggcctt actctctcca tgcagccgcc gatgaaaggc gacaatttct
    23641 tgattatcac caatggtggc ggtaacggtg tgctcgctac tgacgccgcg gaacggttcg
    23701 gcatcccgtt gaaattcgcc ccggatgacg ttcaagccga aatgaagaag catatgcccg
    23761 agtacggttc tgccaagaac cccgtggata tgaccggcat ggcaggcaac gattggtatt
    23821 acgaaaccgt caagttcgct attatgaata agtggacgga tagtgtagtt gtgctgtact
    23881 gcgaaacagc cattactgac cctgcagaaa tcgctgcgag catcaaacgg gctgtggatg
    23941 acagtggcgt aaaagacaaa ccgatcacgg tatctttcgt cggtggtgaa aaatcagata
    24001 aagccatcgg ttggatggtg gaaaacggca tcccggcgtt cttcgcccct gatacagcgg
    24061 taaacgcgat ggcagccctg cgcgaatacg ccaaattaca agagaacaag aaatctgacg
    24121 gcgcgaaaac cgatgaaaaa gccaaaaaac tggcgcttga agtcattgcg gctgcgcgag
    24181 ctgatgggcg tacttcattg accgaaattg aagccaagaa agtctttgag ttatatggta
    24241 tgccaacaac caagattgga ctggcgaaaa ccgaagaaga agccgtcaag ttgagtaaag
    24301 aaatcggtta ccccatcgtg atgaaaattg tctcgccaga tattcttcac aaatcagatg
    24361 caggcggtgt caaagtcaat attaaaaccg aagctgaagc tcgcgaggct tataacacca
    24421 tcctcaagaa cgccaaagcg tataaagcgg atgcgaatat tcatggtatc gccgtgcagg
    24481 aaatggcgcc ctggggcacg gaggttatcc tgggttcggt aagtgaccca tccttcggcc
    24541 caaccgtgat gttcggactg ggaggcatct tcgttgaagt gctcaaagat gtcaccttcc
    24601 gtgtaacccc ggtatcaaaa gccgaagcgg aggagatggt gagtgaaatt cgcggtgccc
    24661 caatcctctc aggtgttcgc ggcgaggccc ctcgcgatcg taaagccatc atcgagacca
    24721 ttatcaaata ctcgagcatg atctatgacc tgatggatga aattgcagaa tctgatgcca
    24781 atccggtgct ggtttacgcg caaggcgaag gactgcgcgt tgctgatgcc aggatcatcc
    24841 tcaagaaaaa gtaactgatc acccgcggaa agagcttttg ccaacaagca gaagctcttt
    24901 tttattttaa atttcttgcc gcccccaccc agggtgtgcc caggcgatgg cgttgcggca
    24961 ccaccacttt gttgagtttt tgccctatat aagcagttgt aactgcaatc gcgccccagt
    25021 cttcgcttaa aaaagcaatt cgcagccttg aagcgctatt acctgcccat tcccattcca
    25081 gcctgaaacg atccgctttc acccagtatc cccgcttctc ccaggcttca accgaggtgt
    25141 caatattcgc ggcaatttct tccagggcga gtatcacata ggcagtgata tccttggtaa
    25201 ggttatccgg ctccgattgt ttcataagtt cgcgtaaagc cagcacgatt gttttcgaaa
    25261 gtctggcgcg ttcttttccg gcgctatccg gtgaataaat ttttgacaaa agaggtacgc
    25321 tccttctcgt gcatttttat acccgcatcg aacgtgcttg attatattcg attattgacg
    25381 cctgccaaac cagcgcttat aatttcagcg cgcaccgggg aaatgctgga attggcagac
    25441 aggcatgact taggatcatg tgccgcaagg cgtgcgggtt caagtcccgc tttccccact
    25501 tgcagacgat tcaagcgaag tattgttgtc caaccatgat aagctgaaca ctatgaaaag
    25561 agaaaacggc aaaatcgcaa tcatcttcat cctgatcatc ctggtcattc tggcggtgtt
    25621 gctctttgct ctggcgccgc aaaacgtcaa ggtgaccgat cctttattca ttgttcagta
    25681 ctggcaaaac atcaccaggg cgctcgcgaa cctcggcggt tttctttcac gctttctcca
    25741 acccatcgga gagggtatcg ttaacttctt ttcaaatatc gggctgccaa aaataaaata
    25801 acctgaacct tttccccaat atcccccgag cttattagaa ctttatatga aactccagcg
    25861 agagtgcgtt ttttatctta atcgcaggat tttgagtata attgggacgc gtttttactt
    25921 gccgccatca atgaccgggg tcatagacgc aacgccagcg ggagttctca ctccctctgc
    25981 cagaaaaaaa gttgaaaagg ataaggaagt tgaaactaaa tatcgaaaca cgtgaagatc
    26041 accaggctaa gatcattgct gaatttgaac cgagcgattt ggataaatat aaaatcaagg
    26101 ccgcccgccg catcgcttcc aagagcaaaa ttcccggttt ccgtccggga aaagcgcctt
    26161 acgatgtggt ctccagactc tacggagaac ccgcgatcga agaagacgcc attgagttaa
    26221 tgatggaagc cgtatacccc gagattctgg ttgaagccaa gatagacccc gcttcttctg
    26281 gcaccctcga agatgtggat aaaggtgatc caatcaaatt cacctttgtc gttcctctcc
    26341 aaccgacagt aaccctggga aattacaagg aaatccgcaa agcctatgcg cccaaatccg
    26401 ttaccgaaaa gcaggtcaat gagttcattc agcacttgcg ccgcacctac gccacagcag
    26461 agcccgttga acgtcctgct gccaaagacg acctggtcta tattaaactg gatgccaccc
    26521 tcttaaagcc tgacaaagaa gaaaacccgg aattattaaa agataggccc ttgcaactgg
    26581 tgattggcga aaatgaccct gaagaaaatg actacccgta cccgggcttc ggcgacaacc
    26641 tgattgggtt aaacgccaac gacgaaaaga aaatcaagta cacctatccc aaggattcca
    26701 agttcgaaaa attacgcggt aaagcagtgg aattcaacgt tcttctccag acagtaaaac
    26761 aactcaccct gcctgaattg aatgatgatt tcgccaagat gtttggagaa tacgaaacct
    26821 ttgcgcagct caaggaagcc atccaggagc aactgcagaa tcgtcagact agtgaatatg
    26881 acgagatgta ttatgaagaa ctgttggatg aggtggtgaa aaccgctacc gtgaaatacc
    26941 cgccgcaggt ccttgagcat gagatggaac acaccatcga aaacgtgatc catgacctct
    27001 ctcaccaaaa aatggaactg gatgtgtacc tgaaaacaat caataaagaa aaggaggttt
    27061 ggctggaaga agaagtcaag ccagcggcag tgaaaaacct ctccaagtca ctcgttttgc
    27121 gtgaactggc caaggctgaa gatatagaac ttgccagtga ggagattcaa acagaagtca
    27181 ctggaatgct tactcagatt cagcaaacca ccaacccaaa agccctcagc aagcagctaa
    27241 aaaataaaaa ctacctcaac gcgctgacaa tggatgcggc ctcgcgcgca atgagccgaa
    27301 aagtgtttga acgtttgaaa gacatcgcca ctggaaaaac agctgaagtg gctggtaaac
    27361 ccgtaaaagc cgctcgttca aaaagcgcaa aagaagcaaa ggactcagtt caatcaactg
    27421 atgaacaaaa acctcaggcg aagaaaacca aagccgccgc tgcaaaagaa gttacgcctg
    27481 ctaccgttaa aaaaacaaag aaggttgaga aaacaagcga ataggagaaa aaatgaattt
    27541 acctgatttc aaaaacgtca tccccatggt cattgaatca tccggccgtg gcgagcgtgc
    27601 gtatgatata tattcactgc tttttcgtga gcgcatcatc ttcgtcggca cccccattga
    27661 cgatcaggtg gcgaatgcca ttgtggcgca gttgctggta ttaagtcgag aagatcccga
    27721 tgcggatatc cagatgtaca tcaactcgcc aggcggtcaa atttactccg ggctggccat
    27781 ttacgacacc atgaagatga tcccaaacaa gatcagcacg gttgccgtgg gcgtgactgc
    27841 ctcttttggt acggtgttgc tcgcagccgg gaccaaagga aaacgctacg cattaccgca
    27901 tgcaactatc cacatgcacc aaccactcgg cggtgctgaa gggcaggcct ccgacattga
    27961 gattcaggcc cgcgagattc tgcgtttgaa atccaggctg atcaacatcc tcgctgaatc
    28021 gaccggtcag aaactggaag tgatcgaaca cgacactgac cgcaacttct acctcgatgc
    28081 tcagcaggcg gttgaatacg gtctggtgga tcaggtgctc acccccccac agccctgaca
    28141 aggcactcgt tcactcgagc cgggtggcag ccgctatccg gctcgtttta tgaggattta
    28201 tggattttga aaaactgaaa agtgtcaaag cattaatagt agacatggat ggtgttcttt
    28261 ggcgcgaatc agcgccaatt ggcaacttac cgcgcatctt taataaaatc aatgcactgg
    28321 ggttaaacta cattctggca accaataatg caacccgcac cccacagcaa tatgtgcaca
    28381 aactcgctgg ctttggtgta aaaattcctg tcgagaaaat tattaattca tcaatggccg
    28441 tagcatccct gcttgccaaa aagcaccccc agggcggagc ggtttatatc gttggcgaga
    28501 acggcttgat agaagccctg gccaatgctg gtttttatcc atccgaatcc aactgcctgg
    28561 ctgtcatcgc cagcatggat cgatgcgtct cattcgagaa gctcaaacgc gccaccctgc
    28621 tgatcagaag cggggtccct ttttacgcta ccaaccccga tcgaacatac cccaccccag
    28681 ccgggttgat tccaggagct ggcgcgctgg ttggagcgct tgaaattgcc actgacgtgc
    28741 atccaattat cgccggaaaa ccaaatccca ccctgtatga gttcgcgctt gaaaggttgg
    28801 gggttacacc agaagtcacc ctggccgtag gtgaccgcct tgaaactgac attttaggcg
    28861 gtcaaagatt gggttgtaaa accgcgcttg tcctttcagg catcgccaca cgggaagagg
    28921 cagaaaaatt caccccgcct gtagatttca tcttccctga cctcgaaagc ctgctagact
    28981 gttatgatgg aaactgataa gtatttgttt gatctcaacc aggatcaact gaaggactat
    29041 ttcgtatcca ttggtgaacc accttaccgg gttgagcaac tatggaacgc ttcctatacg
    29101 cacttcgcgc gagatttcaa ccaggtaacc gccttccctg tgcgcctgcg ttcgcagctt
    29161 gccagcgaca tgtgcttttc tcacctcaag acggtagaga ggttcgcttc tacagatggt
    29221 gaaaccatca aaaatctctt taccctccct gacggtaacc tcatcgaatc tgttctgatg
    29281 cactacaacg agcgccagac gttgtgcatc tcgtcgcaat ccggttgcgc tatgggctgt
    29341 gttttctgcg caaccggtc
//
LOCUS       DAOTLW010000024        28944 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1905_length_28944_cov_9.638098, whole genome shotgun sequence.
ACCESSION   DAOTLW010000024 DAOTLW010000000
VERSION     DAOTLW010000024.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 28944)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28944)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28944
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1905_length_28944_cov_9.638098"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1065)
                     /locus_tag="P5516_05310"
     CDS             complement(<1..1065)
                     /locus_tag="P5516_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91891.1"
                     /translation="MLSFLPVILLLLSAAAIQVVRRMTKRAGSTWLMAMIFGSVTWLS
                     MILVGVLLPPAVKIDNWLSLPHQFTAITFQLTRETWVLGFLLVSLLQAIILFNSRYID
                     TPNFLNKITSSMLLTAFGLMAVLARTPLAFVLTWSLMDLAEFGVLTITLDQTLQTRSS
                     FAAVLLRELGIILLVLLMAVSPEQNLHPGDTTPTLIAWLLGLIFLLRLGILPILPDQE
                     DDSRLRRGFRTLLRTLPFVSAIAFASIFPGLMLAEKGSHIALAALSLAGLFGAILWFF
                     SPDELSGRPYWFFANGLLGLMAFLVNRVEALPGIAVIAVSAGSGLFLYSPKTLKRLAF
                     LPFLLVGLLALPFTPTVGILS"
     gene            complement(1074..2495)
                     /gene="hydA"
                     /locus_tag="P5516_05315"
     CDS             complement(1074..2495)
                     /gene="hydA"
                     /locus_tag="P5516_05315"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HRT91892.1"
                     /translation="MQTLIKNGVLATATKTEEADLLIEDDKIISVGQGLTVEDAVEVI
                     DARGLYVLPGGVDVHVHLDLPMFNTVSSDDHYTGTKAAACGGTTTVIDFVSQETDDLL
                     GCVAKLRQKADARVAVDYGLHMNITHLNPRVEEQIPLLVKEGVTSLKVFTAYNGRLRL
                     QDHEIFRVLRIAGEHGLLTMLHAENGDLIELLVQEACLNDRKSPVWHARTRPAWGAVE
                     AVMRGAALAAVAQAPLYIVHINTSGEVDMLRYARDHGVQLMGETCPQYLFFTEDDLAR
                     PDGAGWVCSPPLRTAADQQRIWKGLKEKTVQVISTDHCPFFLNGTKPILYEGKEVAIP
                     GKELGRDDFTMIPNGLPGVGDRLPVLWTKMVGSGEFSINEFVALTSTNPARIFGMYPR
                     KGSLSPGADADIVLWDPRLEVVYGVAHAQQRTDYNLYEGWKLTGYPLRVLSRGRTVYC
                     DGTWSGKPGNGKFIHRAAFTGMV"
     gene            complement(2561..3142)
                     /locus_tag="P5516_05320"
     CDS             complement(2561..3142)
                     /locus_tag="P5516_05320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91893.1"
                     /translation="MQRSISPNALLLLGILPSPRDLEIARVLGWYRVPLKSSPKVIEV
                     DYLAFYQGANFGEQHRWKVEFFAEYHGHELTTRAELLRDQPDHPRANEEYFKIQLGPI
                     LPVEPPIVSAGWKRITFLFTTGELFNQAETINDLVVRTEERAVLWKKLRERNREMNRY
                     RAGASPNAEIAPEVLDLLLGLNSNLPDTDLENY"
     gene            complement(3202..4668)
                     /locus_tag="P5516_05325"
     CDS             complement(3202..4668)
                     /locus_tag="P5516_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRT91894.1"
                     /translation="MAKIDLQVFEERRQRAIERCRKQNILFPTFAQMKNPDLIPQKIK
                     EELSSIGLWDIHPRNLFRITWKNEPLEKGGGYGGANYLELPTELTGVKARIIALVGKW
                     FPTGAHKVGAAYGCLVPRLVTGQFDPTLQKAVWPSTGNYCRGGAYDSALMACQSIAIL
                     PEGMSRERFEWLNRVAGEVIATPGTESNVKEIFDKCWELRRSGEDLMIFNQFDEFGNY
                     LWHYEVTGHAMEEVLKKEMRPGDRYRGVALTTGSAGTIASGDYLKQLFPESKIIASEA
                     LQCPTLLENGFGAHRIEGIGDKHVPWIHNIKNTDLVVAIDDNAVMEIMRVFNEPEGQK
                     YLASLGLAEALVSKLHLLGFSGIANLLSAIKFAKYYELGEEDIVLTVLTDSMELYESR
                     VVEIQKEKGKLTQLQAAAAFSRYLLGERTDNLIELSYQDRRRVHNLKYYTWVEQQGKT
                     YEEILDQWYDPTYWTDVQGQAEEIDELIIDFNQRTGLL"
     gene            complement(4685..5629)
                     /locus_tag="P5516_05330"
     CDS             complement(4685..5629)
                     /locus_tag="P5516_05330"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT91895.1"
                     /translation="MKPVFSIIAPVYNEIACLDTLLDRIQKVMDQTGEPWELVLVDDG
                     STDGSTDKIRQLAENDQHVRKVILARNFGHQIAVTAGMDYSRGDAVVIIDADLQDPPE
                     VILDLIARWREGYQVVYAQRTEREGETWFKRVTASVFYRSIYRITDIKIPLDTGDFRL
                     LDRKVVNVMNSMRERHRFLRGMSAWVGFKSIGVPYKRAARFAGSTKYPFKKMLKLALT
                     AVTSFSYVPLQLAMWLGFISAGISIITIPVVIVLRLVGSQFFFGQATTLIAVLFLGGV
                     QLISLGILGEYVGRIYDEVKGRPLYTTSETPDDALKTP"
     gene            complement(5626..6888)
                     /locus_tag="P5516_05335"
     CDS             complement(5626..6888)
                     /locus_tag="P5516_05335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRT91896.1"
                     /translation="MNHYLGYRCTFCDTEISPTEILYTCPACGGNLDVVLDYRRIQQK
                     YRPEDIFSRAEGSLWRYEPLLPVTDPGGLATPLHAAGWTPVYQPERLIRGLGLRKFWL
                     KDEGKNPTASFKDRASAIVVARSREIGADVVVTASTGNAGAALAGMAAAIEQNAVILA
                     PKTAPAAKIAQLLIYGAQVVLVDGSYDDAFDLTIQASREFGWYCRNTGYNPFTAEGKK
                     TAAFEIWEQVLRHFRAGNKPLCVFVSVGDGNIISGLHKGFKDLMALGWLERMPRLFGV
                     QSELSSAVADAYLAGTEVITPVKATTLADSISVNLPRDGGRAVRAAVETEGAYIKVAD
                     ADIIRAIIDLGAAGIFAEPAGAAALAGLQKALAEGDLLEDDPVLVINSGNGLKDIRAA
                     MMAVPNPAIIEPTMSALKLHLEKKGRIE"
     gene            complement(6898..7890)
                     /locus_tag="P5516_05340"
     CDS             complement(6898..7890)
                     /locus_tag="P5516_05340"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91897.1"
                     /translation="MEMTTLTTLAGIVEFAVPVLFAAIGENITEKAGLINLSVNGTII
                     LSAMGGFVVALTTSSILLGFLVGAVIGAAVAAIVAYSSITLKQSQVAIGFVLAMLCRD
                     LAYFLGAPFTNKPGIFLSKVPIPYLVDIPIIGPIFFNHSLVVYASYLFIIIAYVYVFK
                     TRPGLMLQGIGEKPAAAFARGANVKGLRYLYTITGGALIGLAGPMYSLAVKIGWAGQL
                     SGLDGIGWIALAITIFGGWNPLRVAIGAYLFGGLQQLGITLQSATNIPIQILQAAPFP
                     LMIFTLLLVNVGRADWVDRELAAMPERPRKVLSKILHALRTSPPSAIGVPFENE"
     gene            complement(7901..8974)
                     /locus_tag="P5516_05345"
     CDS             complement(7901..8974)
                     /locus_tag="P5516_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91898.1"
                     /translation="MSVELRYSRSARSLKKSAWVNVGIRLSAIIMALLFTSVILLIAK
                     ANPLQAYKSILHGAFGSTIKLTSVIASWVPLVIATSGLLFTFTAGLWNIGMEGQIIMG
                     SVFAVGAFRFLDDTSLAPALVITLAFVAGAVGGILWALLPGLMKIFGGVNEIFGGLGL
                     NFVAKTVAIWLIFGPWKRPGVASGSGTEWIDIQYRLPYLPGTNLSLTILIIAVIIAII
                     TAVMLKGTHFGLRLKAIGKNDKAAYILGVPTSRYFLLAFGICGFFAGIAGSILVTSKQ
                     YSLIPDAGFGYGFLGLLVAMLVNYHPIWSSVVALFFAALSIGNTELQYMGLNSYFAGV
                     LQGFLVLFVVLVDGVRKQLVKTR"
     gene            complement(8997..10460)
                     /locus_tag="P5516_05350"
     CDS             complement(8997..10460)
                     /locus_tag="P5516_05350"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT91899.1"
                     /translation="MKISFSHIHKRFGNVHANNDINLVIPSGSIQGILGENGAGKSTL
                     MKILTGYYHADSGEILLDDRVVKITSPVDAVRQGIGMLHQDPLDFPPMRVIDNFLLGS
                     RGGLFPKRSDVEKEILRLSREFDFQISPDTYVETMTVGERQQLEILRLLWLGVKVLIL
                     DEPTTGISANQREKLFATLVKLAKQGLTVIFVSHKLEEVEQLCDRVAVFRQGEVVGEV
                     EPPYDTDQFVYMMFGKSVKVGDKKTFCTELPLIQLENIGLEDYRLHLGNINLEIYQGE
                     VIGLAGMEGSGQRLFLHMLTGLLRPVNGKIKLTGEDFTGKTYPYFQKAGVAYLPAARL
                     EEGLIPGMTLTEHFILSEEHSELFVDQKSALGLTEERIRDFYIKGTPVSMIEQLSGGN
                     QQRALLALLRRKLKLILMEHPTRGLDIESAIWIWSKMKERCKECSSVVFTSSDLDEII
                     QYSDRILVFFGGLVSEPIDATTTTVDELGQLIGGKGW"
     gene            complement(10526..11803)
                     /locus_tag="P5516_05355"
     CDS             complement(10526..11803)
                     /locus_tag="P5516_05355"
                     /inference="COORDINATES: protein motif:HMM:NF014647.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRT91900.1"
                     /translation="MFTKKLWLSLSVLLVAVLVLSACAAPAPTEAPAPEVPAATEAPA
                     ATEAPAATEAPAKAPVIGLLLIGPANDAGWSQAHYESMAYVKDKIPGTELLYLENVYT
                     GSTSHTQTPAELAADMVTKGANIIIFNSDDQKDNAIKFAQEHPDVFVIHPSGDAAWKE
                     GKNYANLPNMKNIMGEMEYGKMIAGCAAALTTQTGKIGFLGPLINEETRRFAASAYLG
                     AKYCWENYLQKDPADLEFKVTWIGFWFNIPGVTLDPTQVSDDFYNTGYDVVISSIDTT
                     EALVEAKRLAADGKKVWAVPYDYYGACEGAQEVCLGVEYFNWGFAYVDAIKSAMDGTW
                     KSSFELVPPDWKDINNPDTSGVGFIKGGGLSAENAAKVDQFIAELGAGLNLWKGPLNL
                     QDGTVYLADGEVATIQQIWYLPKLLEGMEGQSE"
     gene            complement(12083..13039)
                     /gene="arcC"
                     /locus_tag="P5516_05360"
     CDS             complement(12083..13039)
                     /gene="arcC"
                     /locus_tag="P5516_05360"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HRT91901.1"
                     /translation="MTKKLAVIAVGGNSLILDPKKVTIEDQYTAAAETTKHIADMIEA
                     GWDVAIGHGNGPQVGFLLRRSEIAAKYEGMHEVPLDSCGANSQGAIGYWLQQTLQNEL
                     ARRGIKKPVATVVTQVKVDKNDKAFQNPTKPIGGFMDEAEAKKREQELGWNVVEDSGR
                     GWRRVVASPLPVEVVEIDSVRALLNAGVVVITVGGGGIPVVDNGKGGCVGTAAVIDKD
                     YASSLLSTLINADLFIISTAVEKIAINFGKPDQKWLDRMTLAEAKAYLAEGVHFAKGS
                     MAPKIQAIINFLEAGGKEALVTNPENIGRALRGETGTRIVRE"
     gene            complement(13092..14072)
                     /locus_tag="P5516_05365"
     CDS             complement(13092..14072)
                     /locus_tag="P5516_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HRT91902.1"
                     /translation="MHTFLHGRDLIGDLDFSKEEVETVLDVAFDLKRKRALNELTPYL
                     RDKVLAMLFFFSSTRTRGSFEAGMAHLGGHAAFIESRTTQISHGDTPKEIGEIYGRYF
                     DGISIRHCDWGVGNGYLNEVAKASRVPVLNMQCDIYHPFQCLADLMTIMEKKGRDLRR
                     KKMVVSWAYAASYQKPMSVPQSLILQMPRFGMDVVLAHPPEFRLMPEIMEQAREQARK
                     FGVGFDVVEDMDEAFKGGDIIYAKSWGAMVHTPDPEEGAKIIKKYESWITDERRMKLA
                     KPDAIYMHPLPADRNIEVTDAVLDGPQSVVYDEAENRMHVQEAVMALTMG"
     gene            complement(14194..14559)
                     /locus_tag="P5516_05370"
     CDS             complement(14194..14559)
                     /locus_tag="P5516_05370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91903.1"
                     /translation="MSADGISSLAKGCCATPEWTGERFFVLFPGGSLTDDTKDKLRRY
                     NVNFREAKKNMKQVIASADVTLVEPMVRTDHPKHGEEEGERGKTSAVVALTDGWFINK
                     SPLRCHSASLKIAWKWNLP"
     gene            complement(14715..15473)
                     /locus_tag="P5516_05375"
     CDS             complement(14715..15473)
                     /locus_tag="P5516_05375"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRT91904.1"
                     /translation="MSGSPTSKTKRPPFSRLQNELSRLIQRTPPGEKLPSEPVLAKSL
                     GVSRATLREGMRTFEARGLIRRRQGVGTFVVSQLKVIETGLEVLESIERLANRIGLAV
                     RMGELVISETMANPEISERLQVEEGTRLTNIARVIYADDRPVAYLVDILPPNILAESE
                     LQSGFNGSVVDFLIQRGNPGLSKSFTDIQAVAAESSIARLLEIQRGDVLLLFTARLYS
                     DMGEIIDFSYSYFLPGYFRFHVVRNVDTHVAYNV"
     gene            complement(15504..17300)
                     /gene="ade"
                     /locus_tag="P5516_05380"
     CDS             complement(15504..17300)
                     /gene="ade"
                     /locus_tag="P5516_05380"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000034 - adenine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HRT91905.1"
                     /translation="MGMDSFWLQNARELVDCAMGRLPAHLIIKNGQWVCVQTGEIIPK
                     TDVAMRAKRIAYVGEDASHTIGPKTVVIDADGQYLVPGLLDGHVHIESGMVTVTEFVR
                     AVLPHGTTGLFIDPHEIANVFGLRGVRLMVDDAALQPVHVFVQMPSCVPSAPGFETPG
                     AVLGPKDVTEAMTWPGIIGLGEMMDFPGVYEGDDKVHAEMSATRAAGKVIGGHYASPD
                     LGRRFHGYVAGGPQDDHEGTRLEDAVARARQGMKVMMRYGSAWHDVAAQVKAVTELGL
                     SSRQFLLCADDILSETLVNEGHMDRIIRHAIAEGLDPMTAIQMATINTAEHFGVSREI
                     GLIAPGRFGDVVLVKDLRDFQVETVVARGKLAYQKKTILVALPAREYPEWAVRSVHFP
                     RPLSAASFRLGAGGSQEVLANVIGIIENQAPTRHLLFKMTVKDGEIYPDLARDIAKLA
                     LVERHRPTGQVRVGLVHGFGFKIPCAVATTVAHDSHQMLVAGTDETQMAIAANHLESI
                     GGGQVIVTNNTVIGEIRLPIAGLMSTGSAQQVADETASLLEGFKQCGCTLNNPNMQLS
                     LLGLPVIPELRLTDLGLVDVNRFDFIPVLQHE"
     gene            complement(17312..18694)
                     /locus_tag="P5516_05385"
     CDS             complement(17312..18694)
                     /locus_tag="P5516_05385"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HRT91906.1"
                     /translation="MSTLLVKNAHILVTMDDLRREIRGGGLFIRDGFIEQAGATDQLP
                     ATADEVLDLTDHILLPGLVNTHHHFYQTLTRAIPAAQNANLFHWLKTLYPIWAKMTPE
                     DIRLSTQTALAELALTGCTTASDHLYLFPNGSRLDDEIEAAADLGLRLHASRGSMSLG
                     ESQGGLPPDSVVDSEEKILADSERLIQKYHNPNPGSMVQIVLAPCSPFSVTGELMKQS
                     AELARRYGVHLHTHLAETQDEEAFCRQKFGYRPVGYMQSLGWVGEDVWFAHSVHVNDE
                     EIDVYARHGCGVAHCPSSNMRLASGIAPIARLVDAGVKTGLGVDGSASNDGSHLLAEA
                     RMAMLLARLNAGLQGASLSTTSAEIFSVRQALELATRGGAAVLGRKDIGSLEAGKCAD
                     FIAINLNRLDYAGALHDAVAAVLLCQPRGVDFNYVHGKGVVKKGVLSGLNLEAHIHLH
                     NRAATRLFEG"
     gene            complement(18801..19679)
                     /gene="folD"
                     /locus_tag="P5516_05390"
     CDS             complement(18801..19679)
                     /gene="folD"
                     /locus_tag="P5516_05390"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HRT91907.1"
                     /translation="MTATIIDGKAIGDELRREVAAGVQQRLDSGKSRPGLATVLVGEN
                     PASQVYVKSKQKACAEVGIESFGHELPRDASQAEVERLVTQLNADPAVNGILVQLPLP
                     SGLDEEKILSAISIEKDVDGFHPINIGRLAQKGRDPLFVPCTPAGVMYMLEKEFPSLE
                     GLNAVVLGRSNIVGMPVALLLVRANATVTICHSRTKNLPDVVRSADVLVAAVGKAEMV
                     RKDWIKPGAVIIDVGINRVEDATKPRGYRLVGDVCFDEAAEVARAITPVPGGVGPLTI
                     AMLLRNTLRAASLQDA"
     gene            19785..21098
                     /locus_tag="P5516_05395"
     CDS             19785..21098
                     /locus_tag="P5516_05395"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRT91908.1"
                     /translation="MSKICVIGTGYVGLVTGTCFADLGNEVICLDIDHERIASLKAGQ
                     MPIYEPGLEQLVKQNVAAERLFFTTDYAIALKDAEYAFIAVGTPSGNDGEADLRYVRS
                     AAEAIADHVKNAILVINKSTVPVGTGDWVADVINRRRHGKALNFSVVSNPEFLREGSA
                     INDFMNPDRVVLGSEDQSAADRVAQLYQPLRCTIMTTDLRTAEMIKYASNAFLATRIS
                     FINEIANICEELGADVREVARGMGLDKRIGPQFLDAGLGWGGSCFPKDVKALEHMAVL
                     HGTQPQLLQAVMEINRNQRRRAVMKLRKSLKTLNEKTIGVLGIAFKPNTDDIRDAAAI
                     ELIHLLQNEGAQIKAYDPQAMENASHVLKNVVLCENPYQVAEGADALVLATEWNEFKQ
                     LDFKKLKSIMKQAVILDGRNLWDASALKDLGFTYMGIGRYHLPGK"
     gene            complement(21150..24047)
                     /gene="uvrA"
                     /locus_tag="P5516_05400"
     CDS             complement(21150..24047)
                     /gene="uvrA"
                     /locus_tag="P5516_05400"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HRT91909.1"
                     /translation="MTQNCIRVVGARAHNLKNITVEIPRDKLVVITGLSGSGKSSLAF
                     DTIFAEGQRRYVESLSAYARQFLGQMEKPDVDYIEGLSPAVSIDQKATSHNPRSTVGT
                     VTEVYDYLRLLFARIGIPHCPVCGRVVAKQSAQEIVDQVEALPEGSRLLILAPVVRAR
                     KGTYQAAFEEIRKAGFVRVRVDGQVYNLDQDIVLDRYKSHNIDAVVDRIVITRSEDEE
                     EQRSFRSRLTDSIETGLKVGEGNLIVQEVSGDTPLDLFYSEHLACPEHNISIPEIEPR
                     TFSFNTPHGACPECQGLGSKLEIDPDLIIPDPERSMREGAIAALEWNGPRDQGGYYWQ
                     MIEAVAKRYSIDLDAPVGSIPQEKLDKILFGTGSESIPVVMEGRNERKTTMQLSFEGI
                     IPNLERRFRETHSEYIRNRISEFMSDRPCPTCKGERLRPEALAVTVLDHNIVEVTNWP
                     VNRTLEFIASLSTSGVLSNRDFTIAERIIKEITSRLGFLVNVGLDYLTLSRPAGSLSG
                     GEAQRIRLATQIGSRLMGVLYVLDEPSIGLHARDNEKLLETLKQLRDQGNTVLVVEHD
                     EETIRAADWILDLGPGAGEHGGEVVAQGTVDAIINHPHSLTGAYLSGRKKVPLPKKLH
                     PRNGKTLKIVGARANNLKNLEVEIPLGQFVCITGVSGSGKSTLMVDLLYNVLAHELNR
                     ARTQPGEYDRIEGMQYLDKIINIDQSPIGRSPRSNPATYTGMFDEIRSLFASLPESKM
                     RGYKAGRFSFNVHGGRCEACQGQGQLRIEMQFLPDIYVPCDVCHGTRFNRETLQVKYK
                     GLSIADVLDLTVEEGIKLFGNIPAIANKLHTLEKVGLGYIRIGQPGTTLSGGEAQRVK
                     LSKELSRRATGRTMYVLDEPSVGLHAADVHMLIDVLQHLVDSGNSVLIIEHNLDIIKV
                     ADYLIDLGPEGGDRGGELIATGTPREVCDVPTSYTGQVLKAYLQLHQQDPQ"
     gene            24389..25726
                     /locus_tag="P5516_05405"
     CDS             24389..25726
                     /locus_tag="P5516_05405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT91910.1"
                     /translation="MAAMKEKATESERLHKQEIHAWTMYDWANSSFATTMMAAVFPIY
                     FATLGAAGETGAIMTSRWGFTTAFAALLAAVISPLLGAMADFRGSKKKFLTVFMLLGV
                     IATALLFFPNQPGSWLIAAILFIFANIGFAGSLVYYDALLPHVARADEIDQVSSRGYM
                     MGYFGGGILLAVNLVMIMVLPEVFPQLGKGLMTRLSFVTVALWWFGFTLPLLFKVKEP
                     LRRIEEGEEDLHPIRASLIRLKKTFLDLKKYRDLSLGLLAFWIYANGIGTIITMATIY
                     GKELNFSDTTLIGTLLMVQILAAPFAMLFGWLAKKIGTKKAIYLSLVIYSLIAVAGYF
                     LYHEWQFWVLGAAVATVQGGSQALSRSMIGKMMPKSKSAEFYGFFSVFEKFASILGPF
                     IFGVVSHLMNESRLSIASLILFFGLGLFVLTKVNLERGVKVASDEEAKLITVE"
     gene            complement(25787..26194)
                     /gene="mce"
                     /locus_tag="P5516_05410"
     CDS             complement(25787..26194)
                     /gene="mce"
                     /locus_tag="P5516_05410"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HRT91911.1"
                     /translation="MAKIKKINHVAVVVGDVDEALKFWQDAMGIQLHHLEDVPSQKAQ
                     VAFLPVGESEVELVKPTSDDSGVAKFLAERGGGMHHLCFEVDDIAGMMAQLKEKGVRL
                     LSEEPQVLPGRKMVFIHPKSTGGVLVELYEVTK"
     gene            complement(26213..27862)
                     /locus_tag="P5516_05415"
     CDS             complement(26213..27862)
                     /locus_tag="P5516_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HRT91912.1"
                     /translation="MNLESEKKRWDEEVVKPVVEKFKERKDEFTTPSGIPIPRAAIPG
                     SADYMEQLGFPGEYPFTRGVQPTMYRSRFWTMRQYAGFASAEESNRRYRYLLEQGQTG
                     LSVAFDLPTQIGYDADDSMAMGEVGKVGVSISSLRDMETLFAGIPLEKVSTSMTINSP
                     AAILLAMYIAMGKKQGVSPRLLRGTIQNDILKEYVARGTYIFPPKPSLRLITDIFKYC
                     ESEVPNWNTISISGYHIREAGSTAVQEVAFTLANAIAYVQAAIDTGQQVDQFASQLSF
                     FFAAHNDFLEEIAKFRAARRLWARIMRERFGAADPKSWMLRFHTQTGGSTLTAQQPEN
                     NIVRVAIQALAAVLGGTQSLHTNSKDEALWLPTEKSVRTALRTQQIIAYESGVADTID
                     PMGGSYVIEHLTDEIERRVMDYIKKIDDLGGALAAIEKGFMQNEIQDSAYRFQKDVEN
                     KAQLVVGVNAFQSNDTSDLEQLRVDPAIEQQQRVRLAELRGNRDQTKVNELLGHLEST
                     AKASKNLMPLFVTCVENEITLGEICGVLRTVWGEYQPPVWG"
     gene            27977..>28944
                     /locus_tag="P5516_05420"
     CDS             27977..>28944
                     /locus_tag="P5516_05420"
                     /inference="COORDINATES: protein motif:HMM:NF013188.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HRT91913.1"
                     /translation="MSNFLQLIAEIAIILLAAKAAGYLSVRLKQPSVFGELLVGVLLG
                     PSLLDFTNLGFVTSTHLSETIHELGEIGVLLLMFLAGLELNIKDLTRNTRVALLAGML
                     GVVFPVGFGIVFGELLDIPFRQALFLGLTLGATSVSISAQVLIELQALRTRVGLALLG
                     AAVFDDILVILILSSVLAFLSGSGGFREIMWVFTKMVIFLMLAAAVGLWLLPRVVRLT
                     RSLKISQGLTTLALVVLLGYGLAAELMGGMAAITGTFLAGLMFARTPEKGTIETNMHP
                     IAYAFFVPIFFVSIGLNVNLRELNADSIWLFLGICGIAIAGKVIGSG"
BASE COUNT         6804 a         8114 c         7131 g         6895 t
ORIGIN      
        1 agagaggatg ccaaccgtcg gagtgaaagg taaggcgagc aagccaacca gtaagaaagg
       61 aagaaaggct aatctcttca acgttttagg ggaatacaaa aagaggccac tgccggctga
      121 aacggcaatt accgcgatcc ccggcagcgc ctccaccctg ttgacgagaa acgccatcag
      181 cccaagtaac ccattggcga agaaccaata agggcgaccg ctgagctcgt ccggtgaaaa
      241 aaaccacaaa atggctccaa atagcccggc caatgagagg gcagccaggg cgatatgtga
      301 acctttttca gcgagcatca ggcccgggaa aatgctggca aacgcaattg ccgatacaaa
      361 aggaagagtt cgtaacaagg ttcggaatcc cctgcgcagc cgagaatcat cttcctggtc
      421 tggcaggata gggaggatac ccagtcttaa caggaaaatc aatcccagca gccaggcgat
      481 aagagtcggg gtcgtatctc cggggtgcag gttctgctca ggtgaaaccg ccattaacag
      541 caccagcaag atgatcccca gttcgcgtaa caaaacagcc gcaaacgatg aacgagtttg
      601 caaggtctgg tcaagggtga tcgttaagac gccgaattca gccaggtcca tcaaagacca
      661 ggtcaacaca aatgcgagcg gcgtgcgcgc caataccgcc attaacccga aagccgtcag
      721 aagcatagaa ctagtgatct tgttgaggaa attaggcgta tctatatacc ttgaattgaa
      781 caagataatt gcctgcaata aagaaacaag cagaaaaccc aagacccagg tttctctggt
      841 caactgaaat gttatcgcgg tgaactggtg aggtaaagag agccagttgt caatcttgac
      901 cgccggaggg aggagcacac ccacaaggat catgctcaac caggtgacag agccaaatat
      961 catggccatt aaccaggtgc tgcctgcacg tttggtcatc cttctgacaa cctggatcgc
     1021 agcagctgaa agcaacagga gaataaccgg taagaaagac aacattgcgc ttcctaaacc
     1081 atccctgtga atgcagcgcg gtgaataaac ttaccattac ccggcttacc tgaccaggtt
     1141 ccatcgcagt acacagtccg gccgcgagaa agcacccgca gcgggtaacc ggttaatttc
     1201 caaccttcat acaggttgta gtctgtgcgc tgttgcgcgt gagccacccc atacaccact
     1261 tccaaacgcg gatcccataa cacgatgtca gcgtccgcgc cgggtgataa cgaacccttg
     1321 cggggataca taccgaagat cctggcaggg ttcgtactgg taagtgcgac aaactcgttg
     1381 atcgaaaatt caccagaacc gaccattttc gtccacaaaa ccggtaaacg gtcgccaaca
     1441 cctggtaagc cattgggaat catggtgaaa tcgtctcgtc ccaactcctt tccggggatg
     1501 gctacctctt ttccttcgta aagaatcggt ttcgtgccat tcaaaaagaa ggggcaatgg
     1561 tcggttgaaa tgacctgaac cgttttttct ttcagacctt tccaaatgcg ctgctggtct
     1621 gcggcagtcc tcaatggcgg agagcaaacc caccctgcgc catctgggcg ggcaaggtca
     1681 tcttcggtaa agaacaggta ttgcgggcag gtttcaccca tcagctgcac accatgatcg
     1741 cgggcatacc gaagcatatc cacttctccg gaggtattga tatgcacgat atacaagggc
     1801 gcctgggcaa cagcggctaa cgcagccccg cgcatcaccg cctctactgc gccccaagcc
     1861 ggtctggtcc gcgcatgcca aacagggctt tttcggtcgt tcaggcaggc ttcctgaacc
     1921 agcagctcga taagatcacc gttttccgca tggagcatcg tcaaaagccc atgttcaccg
     1981 gctatcctca gcacacggaa gatttcatgg tcctgcaggc gcagccgtcc attgtaggcg
     2041 gtaaaaactt tcaggctggt cacgccttct ttgaccaaga gaggaatttg ctcctcgacc
     2101 cgcgggttga gatgggtaat attcatatgc agcccataat caaccgccac cctggcatct
     2161 gccttctgcc gcaacttggc cacgcatccc agcaggtcat ctgtttcttg tgaaacgaaa
     2221 tcgatcaccg tggtggttcc tccgcatgca gcggcctttg ttccggtata atgatcatct
     2281 gatgacacgg tattgaacat cggcaggtca agatgcacgt gaacatccac gcctcccgga
     2341 agcacgtata atcctcgggc atcgatgact tcaaccgcat cttccaccgt caagccctgc
     2401 ccgacgctga tgatcttgtc atcctcgatc agcagatctg cttcttcggt tttcgtagcg
     2461 gttgccaata caccgttttt tatcaatgtt tgcatcttgt tttcctgatg actcgcctag
     2521 gtatttctct ttttttattc taccagcatt agccgggcgg ttaataattt tccaggtcgg
     2581 tgtcaggaag attagaattc aagcccaaca gcaggtccaa tacctcaggc gcaatctccg
     2641 cattcggaga agcccctgcg cgatagcgat tcatctcccg gtttcgctcg cgtaattttt
     2701 tccacaagac ggcgcgctct tctgtgcgca cgaccaggtc attgatcgtt tcagcctggt
     2761 tgaataattc acccgtggta aaaagaaatg tgatacgttt ccagccagcg gagacgattg
     2821 gaggttcaac tggcagaatt ggccccagtt ggatcttgaa gtattcttcg ttcgccctgg
     2881 gatggtctgg ctggtcgcgc aacaattccg ctcgtgtggt cagttcatgt ccgtggtact
     2941 cagcgaaaaa ttcaaccttc caacgatgtt gctcaccaaa gttcgcaccc tgataaaacg
     3001 ccaggtagtc cacttcaatg accttgggag aggactttag cggaacacga taccatccca
     3061 gcacccgggc aatttcaaga tccctgggcg agggtagaat tccgagcagt agtagcgcgt
     3121 taggagagat gcttctttgc atttgaattc agtttaatta atctcgccct gaaagcaaag
     3181 attctcagga taagcgaaat actaaagcag tccagtgcgc tgattgaaat caattatcaa
     3241 ttcatcaatc tcttccgcct gaccttgaac gtccgtccag taggtagggt cataccattg
     3301 atccagaatt tcttcgtatg ttttcccctg ctgctcaacc caggtgtaat atttcagatt
     3361 gtgcacccgt cttctgtcct ggtaggataa ttcgatcagg ttgtcagtcc tctcgccaag
     3421 caggtagcga gaaaaagcag cagcagcctg gagttgagtg agcttacctt tttctttttg
     3481 tatttcaaca acacggcttt catacagttc catagagtcc gtgaggacgg tgaggacgat
     3541 atcctcttct cccagctcat agtatttcgc aaatttaatc gcagaaagca ggttggcgat
     3601 ccctgaaaaa cccaacaagt gtaatttaga taccagggct tctgcaaggc ccaggctggc
     3661 cagatatttt tgaccctcag gctcattaaa aacacgcatg atttccatga ccgcattgtc
     3721 gtcgatcgcc acaaccaggt ccgtattctt tatgttatgg atccagggca cgtgtttatc
     3781 accgataccc tcgatgcgat gggccccaaa gccattttcg agcaaagtag ggcattgcag
     3841 cgcttcgctg gcgatgatct tgctttccgg aaacaactgc tttaagtaat cgccgctggc
     3901 gatggtgccg gcagacccgg tggtcagcgc aacccccctg tacctgtcgc ctgggcgcat
     3961 ttccttttta agcacctcct ccatagcatg cccggtgacc tcataatgcc acagataatt
     4021 accaaactcg tcaaactggt taaagatcat caaatcttca ccagaccggc gcagctccca
     4081 gcatttatca aaaatttcct taacattaga ctctgttcca ggcgtcgcga tcacctctcc
     4141 ggccacacgg ttgagccatt caaagcgctc acggctcatc ccctctggca gaatggcaat
     4201 tgactggcaa gccattaagg cggagtcata ggcgccgccg cggcagtaat tacccgtgga
     4261 aggccacacc gccttttgaa gcgtcgggtc aaactgcccc gtaaccagcc ggggcaccag
     4321 gcagccgtag gctgcgccaa ccttgtgcgc gccggttgga aaccatttgc ccaccagggc
     4381 gatgatccgc gccttaaccc cggttaattc agttggaagc tctaaataat tcgctccacc
     4441 gtatccgccg cctttttcca aaggttcgtt tttccaggta atgcgaaata ggttgcgggg
     4501 atgaatatcc cacaaaccaa ttgaagagag ttcctctttg attttttgcg ggatgagatc
     4561 agggttcttc atttgagcga aggttggaaa caggatattc tgcttccggc aacgttcaat
     4621 ggcgcgctgc cggcgttctt caaaaacttg taggtcgatc ttagccatga ggttgtctct
     4681 cctgtcaggg tgttttcagt gcatcatctg gggtttcgct ggttgtgtac aatgggcgac
     4741 ctttcacttc atcataaatt cggccgacat actcccccaa aataccaagc gagattaact
     4801 gcacgccgcc aaggaaaagc acagcgatga gcgttgtcgc ctgtccaaaa aagaattgcg
     4861 atccaaccag cctgagtaca atgaccacag gaatggtgat gatactgatc ccagctgaaa
     4921 tgaaccccag ccacatagcc agctgcaatg ggacataaga aaaactggtc accgccgtga
     4981 gggcaagctt gagcattttc ttgaacgggt atttggtgga gccggcaaaa cgagccgcgc
     5041 gtttgtacgg caccccgatc gatttaaacc ccacccaggc tgacatgccg cgtaaaaacc
     5101 ggtgccgctc gcgcatgctg ttcatcacat tgaccacttt acggtcgagt agtctgaaat
     5161 ctcccgtatc gagtggtatc ttaatatccg tgatgcggta aatagagcgg taaaaaacag
     5221 atgcagttac gcgtttgaac caggtctccc cttcgcgctc agtccgctgg gcatatacca
     5281 cctggtatcc ctcgcgccac ctggcaatta aatccaatat tacctccggc ggatcctgta
     5341 aatccgcgtc gataatcacc acagcatcac cccgggagta gtccatcccg gctgtcacag
     5401 cgatctggtg accaaagttg cgcgcgagaa tcaccttacg cacatgctgg tcattttcag
     5461 cgagctggcg gattttatcc gtagagccgt cggtcgagcc gtcatccacc aggacaagct
     5521 cccatggctc tcctgtttgg tccatcacct tctggatcct gtccagcagg gtatccaggc
     5581 aggcgatttc gttatagacc ggggcaataa tcgaaaaaac tggtttcatt caatacgacc
     5641 ttttttctca aggtgaagtt ttaaggctga catggtcggt tcaattatag ccgggtttgg
     5701 aaccgccatc atggccgcgc gaatgtcttt aagaccgttt ccgctgttga tcaccagcac
     5761 cgggtcatcc tccaggagat ctccttcagc aagcgctttt tgcagccccg ccaaggcagc
     5821 cgccccggct ggctcggcaa agatgcctgc tgcccccagg tcgatgatcg cacggatgat
     5881 atcggcgtca gcgaccttaa tatacgctcc ttctgtttcc acagccgcac gcactgctct
     5941 gccgccatcg cgaggcaggt tcacactgat ggagtctgcg agcgtggtcg ccttgactgg
     6001 tgtgatcacc tcagtacccg ccaggtaagc gtccgccacc gctgaagaca gctccgactg
     6061 cacgccaaac agcctgggca ttcgctccag ccaccctaga gccatcaggt ctttgaaccc
     6121 tttatgcaac ccggaaatga tgttgccatc tccaacagag acaaaaacgc ataatggttt
     6181 attgcctgcg cgaaagtgac gcagtacctg ttcccatatt tcaaaggcag cggttttttt
     6241 gccttcggct gtaaaagggt tatacccggt attacggcaa taccagccga attctcgcga
     6301 agcctggatg gtcaggtcga aagcgtcatc ataactgcca tccaccaaaa caacctgtgc
     6361 cccgtagata aggagctgcg caatctttgc tgctggtgcg gttttgggcg cgagaattac
     6421 ggcgttctgt tcgattgccg ctgccatacc cgccagcgcc gcgccggcgt tgccagtgga
     6481 agcggttacc accacatcag cgcctatttc tctcgacctg gctaccacaa tagcactggc
     6541 gcggtctttg aaagaggcgg tagggttttt cccttcgtct ttcagccaaa atttccgcaa
     6601 acccaggccg cgtattaacc tctctggctg gtaaacaggg gtccaccctg ccgcgtgaag
     6661 aggggtggct aagccaccgg gatcggttac cggcagtaac ggctcgtatc gccataaaga
     6721 gccttcagcc ctggaaaaga tatcctccgg gcgatacttc tgctggatgc gcctgtaatc
     6781 cagtaccacg tccaggttcc cgccgcacgc cgggcaggta tagagaattt ccgtggggga
     6841 aatttcagta tcacaaaacg tgcacctgta gcccaggtag tgattcatgc cgcctgctta
     6901 ttcgttctca aaaggcactc cgatggctga agggggcgag gtgcgcagcg cgtggagtat
     6961 ttttgaaaga accttgcgcg gtctttcagg catggcggca agctcccggt ctacccaatc
     7021 cgcccggcct acattgacca acaagagtgt gaaaatcatc aacgggaaag gagcggcctg
     7081 taaaatctga attggaatat ttgtcgcgga ctgcagggtg atacccaatt gttgcaagcc
     7141 gccaaacagg taagcaccaa ttgccaccct caacgggttc cacccgccaa aaatggttat
     7201 cgccagggcg atccacccga tcccatccag ccctgaaagc tgacccgccc aacctatctt
     7261 cacagccagg gaatacatcg gcccagccaa gcctatcaac gcaccgccag tgatggtgta
     7321 caaatagcgc agccctttaa cattcgcccc gcgggcgaaa gccgccgctg gtttttcgcc
     7381 aattccctgc agcattaaac ccggacgcgt tttgaacaca taaacatacg cgatgatgat
     7441 aaacaggtat gaagcgtaaa caaccagtga atgattgaag aaaatcgggc caatgatggg
     7501 aatatcaaca agatagggaa tgggtacttt ggaaagaaaa ataccaggtt tgttggtgaa
     7561 gggcgcgccg agaaaatacg ccaggtcacg gcaaagcatt gccagcacaa atccaatcgc
     7621 tacctgagat tgtttgagcg tgatgctgct gtaagccact atggcagcca ccgcggctcc
     7681 gatgaccgct cccacaagga aacccagcaa gatgctactg gtcgtgagcg ccactacgaa
     7741 tccgcccatt gcgctcagga ttatcgtgcc attcactgaa aggtttatca aaccggcttt
     7801 ttcggtgatg ttctcgccaa tcgcagcgaa aagcaccgga accgcgaatt caacgattcc
     7861 cgccagtgtt gtgagagttg tcatttccat caagccgctc ctaacgcgtt tttaccagtt
     7921 gtttgcgaac gccatctacc agcaccacaa ataaaaccag gaaaccctgc agcacaccgg
     7981 cgaaatacga gttcagaccc atgtattgca gttctgtatt gccgatgctg agcgcggcga
     8041 aaaagagcgc aaccactgac gaccaaatcg ggtggtagtt gacaagcatt gccacgagca
     8101 ggcccaggaa accatatcca aatccggcgt ccgggataag gctgtattgc ttggaagtca
     8161 ccaggatgga tcctgcgatg cccgcgaaga agccgcaaat cccgaaagcc aacagaaagt
     8221 aacgggaagt aggaacaccg aggatgtaag cggccttgtc atttttgcca atggctttca
     8281 ggcgcagccc aaaatgggtg cccttaagca tgaccgcagt aataatggcg ataatgacag
     8341 cgataatgag aatcgtcagg ctaagattgg ttcctggcag gtaaggcagg cggtattgaa
     8401 tatcaatcca ttccgtcccg ctccccgatg ccacaccggg gcgcttccac gggccaaaga
     8461 tgagccagat agccactgtt ttcgccacga agtttaaccc aagcccgcca aatatctcgt
     8521 taacaccgcc aaatattttc attaaaccgg gtaacaaagc ccagagaatt ccgccaaccg
     8581 cgccggcgac gaatgccagg gtgataacga gagcgggcgc cagcgaggtg tcatctaaaa
     8641 aacgaaatgc gccaaccgca aagaccgacc ccataatgat ctgaccttcc atgccgatat
     8701 tccacaatcc agcggtaaag gtaaacagca atcccgaagt ggcgatcacc agcggcaccc
     8761 acgaggcaat caccgaggtc aatttaatgg tagaaccaaa agcgccatga agaatacttt
     8821 tataagcctg caatggatta gccttggcaa tgagcaatat caccgacgtg aacaataagg
     8881 ccataataat ggcagaaagg cgaatcccca catttaccca ggcacttttt ttcaatgacc
     8941 tggctgatcg gctgtatctc agttctacag acatccagat tctccgtcac acataattac
     9001 catcccttac cgcctatgag ttgacccaac tcatccacag ttgtggtagt cgcgtcgatt
     9061 ggttctgata ccagcccgcc aaaaaacacc agaatgcgat cgctgtactg aatgatttca
     9121 tccaggtcag aagacgtgaa aaccacactg gagcactctt tacagcgctc tttcattttt
     9181 gaccaaatcc agattgccga ttcaatatcc aatccccggg tcggatgttc catcaagatc
     9241 agtttcaatt ttctgcgcag caacgccagc aatgcacgct gctggttgcc gcctgaaagc
     9301 tgctcgatca tactcaccgg ggttcctttg atgtagaagt ccctgatcct ttcttctgtc
     9361 aagcccaaag cgcttttctg atctacaaat aattcactat gctcttctga aaggatgaaa
     9421 tgttcggtca acgtcattcc cgggatcaga ccttcctcca gcctggcagc cggcagatag
     9481 gccaccccgg ccttttggaa atagggatag gttttaccag taaaatcttc accggtcaat
     9541 ttgattttac cgttcacagg gcgcagcaag ccggtcagca tatgcaagaa cagcctctga
     9601 ccagagcctt ccatacccgc caggccgatg acctctccct ggtaaatttc aaggttgatg
     9661 ttaccgaggt gcaggcggta atcttccaac ccgatatttt ctaattgaat gagcggcagc
     9721 tcagtgcaaa atgttttttt atcccccact ttcactgact tcccaaacat catatacaca
     9781 aattgatccg tgtcataggg tggttcgact tctccaacga cttccccctg ccggaaaacc
     9841 gccacccggt cgcacaactg ctcgacttcc tccagcttgt gcgaaacaaa aatcacagtc
     9901 aaaccttgtt tagccagttt aaccagggtg gcaaataact tttctctctg attggctgaa
     9961 atgccagtag tgggttcatc aaggatcagc accttcacgc ccagccacag cagtcgcaga
    10021 atctcaagct gctgccgctc tcccaccgtc atcgtttcaa cataagtatc cgggctgatc
    10081 tgaaaatcga attctcgtga taaccgaagg atctcttttt ccacatcact tcgttttgga
    10141 aataaaccgc ctctggagcc aagcaggaag ttgtcgatca ccctcatagg tggaaaatcc
    10201 aaagggtcct ggtgcagcat gccaatgccc tgtcttaccg cgtccacagg tgaggtgatc
    10261 ttcaccaccc ggtcatccag cagaatttcg cctgaatccg cgtggtagta cccggtaagg
    10321 attttcatca gggtgctttt tcctgcgcca ttttcaccca ggatgccttg aatgcttcct
    10381 gatggaatta ccaggttgat atcgttattg gcgtgcacat ttccaaaacg tttatgaata
    10441 tgtgaaaatg aaattttcaa gcgtgaacct cgtatttata aaaacgtggg ggaagggaaa
    10501 cccttccccc acgcggcgtt ctatactact cgctctgacc ttccattcct tcaagcaatt
    10561 tcggcaggta ccaaatttgc tgaattgttg ccacttcacc atccgccagg taaaccgtgc
    10621 catcttgtag gttcaatgga cctttccaca ggttcaagcc agctcccagt tcagcgatga
    10681 actggtcaac cttggcagca ttttccgcgg aaagaccgcc acctttgatg aagccaacac
    10741 ccgaagtatc ggggttgttg atatccttcc agtccggtgg caccagctca aagctggatt
    10801 tccaggttcc gtccatggca gatttgatgg catccacata agcgaagccc cagttgaagt
    10861 attccacgcc caggcagact tcttgcgcgc cttcgcaagc gccgtaatag tcataaggaa
    10921 ccgcccaaac cttcttaccg tcagcagcga gcctcttggc ttccaccagc gcttcagtgg
    10981 tatcgatcga gctgatcacc acatcatatc cggtattgta aaagtcatcc gagacctggg
    11041 tcggatccag agtcacgccg gggatattga accagaaacc gatccaggtg accttgaatt
    11101 ccaaatcagc aggatctttt tgcaggtagt tttcccagca gtacttggcg ccgaggtacg
    11161 ctgaagccgc gaagcggcgt gtttcctcat tgatcaatgg accaaggaag ccaatttttc
    11221 ctgtctgcgt tgtgagcgcc gcagcgcagc cggcgatcat ttttccgtat tccatctcgc
    11281 ccatgatgtt cttcatgttc ggcaagttag cgtaattctt gccttctttc caggcggcat
    11341 ctcccgaagg atgaatgaca aacacatccg ggtgttcctg agcgaactta attgcgttat
    11401 ctttttgatc atctgagtta aagatgatga tattggcgcc tttggtcacc atatccgcgg
    11461 ccagttcggc gggagtctgt gtatgcgagg tggacccggt atacacattc tccaggtaaa
    11521 gcagttcggt gccgggaatt ttgtctttga cgtaagccat gctttcataa tgcgcctggc
    11581 tccagccggc atcattggca gggccgatca gtaacagccc aatcaccggg gccttcgcag
    11641 gggcttctgt cgctgcgggt gcttcggtgg cagcgggtgc ctcagtggct gctggaacct
    11701 caggtgctgg cgcttccgtc ggggctggcg cagcgcaggc tgaaagaacc agcaccgcca
    11761 ccaataacac gctcaggctt aaccacaact tttttgtaaa cattgttcac tcctcctgtt
    11821 tctagttagc aaattcaaac atcaacttac cgtgaaagca aggaaccagt tattggtttt
    11881 tgcacctcct ttcgactaga aaacgtaaat tcttgatagg atgaccctcc atcccaccaa
    11941 gcgagtgata ggaaacaaca taattattgt acacgatgcg ctcatgaatg ccatagcaaa
    12001 acaagtaaaa aaaccttaaa aaatcaagaa gggcctgcgg ttttccccgt caaagccctt
    12061 cttgcgtgtt tctcattcgc gactattcac gaacgatacg agttccagtt tctccacgca
    12121 gcgcgcgccc gatgttttct ggatttgtca ccagggcttc cttaccacct gcttcaagga
    12181 agttgatgat agcctgtatc ttgggcgcca tagagccttt ggcaaaatgc acgccttctg
    12241 ccaggtaggc tttcgcctcc gccagggtca tgcggtccaa ccatttctgg tcgggttttc
    12301 caaaattaat ggcgatcttt tcgaccgcgg tggagatgat gaaaaggtcc gcattgatga
    12361 gagttgagag caggctgctg gcgtaatctt tatcgatgac cgccgcggta cccacacaac
    12421 cgcctttgcc gttgtcaacc acaggaatac caccgccgcc aaccgtgatg accacaacac
    12481 cagcattaag gagcgcgcgc acagagtcaa tctccaccac ttctactggg agaggagagg
    12541 cgaccacgcg gcgccaaccg cgcccagagt cttctaccac gttccagcca agttcttgct
    12601 cgcgcttttt tgcttctgct tcatccataa agccgccgat tggtttggtg gggttctgga
    12661 aggctttgtc attcttgtcg actttcacct gggtgaccac cgtggcaacc ggttttttga
    12721 tacctcttct ggctaattcg ttctgcaggg tttgctgcaa ccagtagcca atggcgccct
    12781 ggctgttcgc gccacaggaa tccagcggaa cctcgtgcat gccttcatat ttagccgcaa
    12841 tttcggagcg gcgcagaagg aatccaacct gcgggccatt tccatgacca atggccacat
    12901 cccagccggc ttcgatcatg tccgcaatgt gcttcgtggt ttctgcggcg gctgtatatt
    12961 gatcctcgat ggtaactttt ttgggatcga gaatgagaga attaccacca actgcgataa
    13021 ctgccaactt tttcgtcatt ttttacaaat tccttttcta ttaaaattaa atttgatttc
    13081 tgcgcatgtt cttaacccat ggttaaggcc atcacagctt cttgaacgtg catgcggttc
    13141 tccgcttcgt cgtataccac tgattgcgga ccatccaata ccgcgtcagt gacctcgata
    13201 ttacggtcag ccggcaacgg gtgcatataa atcgcatcag gttttgccaa cttcatcctg
    13261 cgctcatctg tgatccagct ctcatacttc ttaataatct tggctccttc ttcagggtca
    13321 ggcgtatgca ccatggcgcc ccaggatttg gcatagatga tatctccgcc tttgaaagcc
    13381 tcgtccatgt cttcgaccac atcaaaaccc actccgaact tgcgggcttg ttcgcgcgcc
    13441 tgctccatga tctccggcat taacctgaac tcaggaggat gagccagcac cacgtccatg
    13501 ccaaagcgcg gcatctgtaa gatcaaagat tgtggaaccg acatgggttt ttgataggag
    13561 gccgcgtacg cccaggagac aaccatcttt ttgcgccgta aatcgcggcc cttcttctcc
    13621 ataatggtca tcaaatcagc caggcattgg aaggggtgat agatatcgca ctgcatattc
    13681 agtacaggca cgcgcgaagc cttggcgact tcgttcaggt atccgttgcc taccccccaa
    13741 tcacagtggc ggatagagat gccgtcaaag tagcgcccgt aaatctcgcc aatctccttt
    13801 ggcgtatcac cgtgagaaat ctgcgtcgtg cgggactcaa tgaatgccgc gtgtccgcca
    13861 agatgggcca tgccggcttc gaacgagccg cgcgtgcgcg tactgctgaa gaagaacagc
    13921 atagcgagca ctttatccct caaataaggg gtcaattcgt tcaacgcgcg tttccgcttt
    13981 aggtcaaacg ccacatctaa aacggtctca acttcctcct ttgagaaatc cagatcccct
    14041 atcaaatcac gtccatgtag aaaagtatgc atccttcctc ctgatcagtt gattattcca
    14101 tctttccaag aacaagcgcc agaaacgcta tgcgcattac cacatcattg aacgcttatt
    14161 taccgctaac gcgattgccc aacggctcac acttcagggc aggttccact tccatgctat
    14221 ttttaatgaa gcagagtggc atcgtaacgg gcttttatta ataaaccatc cgtctgtgag
    14281 tgcaacgacc gccgatgtct ttccacgctc tccttcttct tcaccatgct tggggtgatc
    14341 tgtccgcacc ataggttcaa ccagggtcac gtccgcggat gcaatcacct gtttcatatt
    14401 tttttttgct tccctgaagt tcacattata ccgccgaagt ttatctttag tgtcatcggt
    14461 caatgaacca ccagggaata aaacaaagaa gcgctctcct gtccattcag gcgtcgcaca
    14521 gcagcctttc gccaaagacg aaatcccatc cgcgctcatt catacagaaa agaattccac
    14581 tcgtcagaat cctttttgtg tgctccctcc ttttcgctga atcattcctt tcctttgctc
    14641 gttattccaa atggattttc gatcggtttg ttttcgtcgt tcaccaatat cctggcaggc
    14701 gtctttttca ccttttaaac gttgtacgcc acgtgggtat ccacatttcg aactacgtga
    14761 aacctgaagt accctggcag gaaatagctg tatgagaaat cgatgatttc gcccatgtca
    14821 ctatacaagc gcgcggtaaa taacaacaac acgtcgccac gctgtatctc cagcaacctg
    14881 gctatactgg attcagccgc aacagcctgg atatcggtaa aagatttaga taaacccggg
    14941 ttaccccgtt gaatgaggaa gtcaaccact gatccgttga aaccggattg cagctctgat
    15001 tcagccaaaa tattcggggg cagaatatct accaggtaag ccactggacg gtcatccgcg
    15061 tagatcaccc gtgcgatatt agtcagtctg gtgccttctt cgacctgcag tctctctgag
    15121 atttcaggat ttgccatcgt ttcggatatg accagttctc ccatccttac cgccagccct
    15181 atgcggttgg ccagtctctc gatgctctcc agcacctcca ggccggtttc gatgaccttc
    15241 agctgagaga caacaaaagt tccaacgccc tgtctgcggc ggatcaagcc cctggcctca
    15301 aaggtgcgca tgccttcgcg caacgttgcc ctggaaacac caaggctctt tgccaaaacg
    15361 ggttcagagg gtaatttttc gcctggcggg gttctttgaa ttaatcgcga cagctcgttc
    15421 tgcaagcgag aaaaaggagg ccgctttgtc tttgaagttg gactgccact catcgtcaat
    15481 cgttccttgc ttgttttggg tttttactcg tgctgcaata ccgggataaa atcgaaccgg
    15541 ttaacatcca ccaaccccag gtctgtcagg cgcagttccg ggatgaccgg cagccccagc
    15601 aaactgagct gcatgttggg gttgttcaag gtacagccac attgtttaaa accctcaagt
    15661 aacgacgccg tttcatcagc cacctgctgt gcgctgccag tagacattaa ccctgcgatt
    15721 ggcagtcgaa tttcaccaat cacggtgtta tttgtgacaa tcacctgacc tccgcctatc
    15781 gactccaggt ggttggccgc aattgccatt tgcgtttcat ctgtacctgc cacgagcatc
    15841 tgatggctgt catgtgctac agtagtggct acagcgcagg gaatcttgaa cccgaagccg
    15901 tgcaccagcc ctacccgcac ctgcccggtc ggccggtggc gttccaccag cgccagcttg
    15961 gcgatatctc tggcgagatc cgggtaaatt tccccgtcct taaccgtcat tttgaataac
    16021 aggtggcgag tgggggcctg gttttcaatg atcccaatca cattcgctaa aacttcctgg
    16081 ctgccacccg cgccaagcct gaaactcgcg gcgctcaatg gcctgggaaa atgcaccgac
    16141 ctgaccgccc attcgggata ctcgcgcgca gggagagcca ccaggatggt ctttttctga
    16201 taggccagtt tccccctggc aaccactgtt tcgacctgaa aatcgcgcag gtctttcacc
    16261 aacaccacat cgccaaacct gcccggggca atgagcccga tttcacgcga aacgccgaaa
    16321 tgttcggcgg tatttatcgt tgccatttgt attgcggtca tcgggtccaa accttcagca
    16381 atggcatggc ggataatcct atccatatga ccttcattaa ccagtgtctc tgataggata
    16441 tcgtctgcgc ataaaaggaa ttgccggctg gaaagtccca actccgtaac cgctttaacc
    16501 tgcgcggcca catcgtgcca ggctgaccca taccgcatca tcaccttcat accctggcgg
    16561 gcgcgagcga ccgcatcttc caacctggtc ccctcgtggt catcctgagg gcctccggca
    16621 acatacccat gaaagcgcct tcccaggtca ggcgaggcgt aatgtccgcc aattactttg
    16681 cccgccgccc gtgtggcact catttcagcg tggaccttgt catccccttc atacacaccc
    16741 gggaaatcca tcatctcccc cagcccaatg atccccggcc aggtcatggc ttcggtcaca
    16801 tccttaggtc ccagcacagc gccgggggtt tcaaaaccgg gggctgacgg gacacaggaa
    16861 ggcatttgca caaaaacatg cacaggctgc aacgccgcat catccaccat caggcgcaca
    16921 ccacgcaaac caaaaacatt ggcgatttcg tgcggatcaa tgaatagccc agtcgtgccg
    16981 tgagggagca ccgcgcgcac aaactcggtg acggtgacca taccggactc aatatgaacg
    17041 tgaccgtcca gcaagcccgg caccaggtat tgaccatccg cgtcaatgac cacggttttt
    17101 ggcccaatgg tatggctggc gtcttcgccc acataggcaa tgcgtttggc gcgcatggcc
    17161 acatccgttt tggggataat ctcgcccgtt tgcacgcata cccattgacc atttttgatt
    17221 atcaagtgcg cgggaagacg acccatcgcg caatcgacga gttctcgtgc gttctgcagc
    17281 cagaaagaat ccatgcccat gcgaaacccc tttatccttc gaacaacctg gtggcggcgc
    17341 ggttatgcag gtgaatgtgt gcttctaaat tcagcccgga tagcacgcct ttttttacca
    17401 cgcctttgcc atgcacataa ttgaagtcca cgcccctggg ctgacaaagc agtacagcag
    17461 ccacggcatc gtgcagcgcg ccggcgtaat ccagccggtt aaggttgatg gcgataaaat
    17521 cagcgcattt gccggcttcg agagaaccaa tatccttcct tcctaacacc gcggcgccgc
    17581 ccctggtagc cagctccagc gcctgacgta cagagaaaat ttccgcgctg gtcgttgata
    17641 aggaggcccc ttgcagaccg gcgttcaaac gcgccagcag catggccatg cgggcttcgg
    17701 caagcaaatg tgagccgtcg ttgctggctg agccatccac ccccaggccc gtttttaccc
    17761 cggcatctac cagccgggca atcggagcga tgccggaagc caggcgcata ttagaagaag
    17821 ggcagtgcgc caccccgcaa ccgtgccggg cataaacgtc aatttcctcg tcgttgacgt
    17881 gaacagaatg cgcgaaccac acatcctcgc ccacccaacc cagggattgc atgtatccga
    17941 ctggtcgata accaaatttt tgccggcaaa acgcttcctc atcctgggtt tcagccaggt
    18001 gtgtatggag atgaacgcca taacgcctgg caagttccgc gctctgtttc attaattcac
    18061 cggtcacgct aaacggggaa cagggggcga gcacgatctg caccatggaa ccaggattgg
    18121 gattatggta tttctgaatc agtcgctcgc tatccgccag tatcttctct tctgaatcca
    18181 ccacgctatc aggcggtaac ccgccctggc tttcgccgag actcatcgat cctcgcgatg
    18241 cgtgcaggcg caaacctagg tcagcagctg cttcaatctc atcatccagc ctggacccgt
    18301 tcggaaagag gtacagatga tcagaagcgg tggtgcagcc tgtcagggcc agctcagcca
    18361 gggctgtttg cgtggaaagg cggatatctt ccggggtcat tttcgcccat atcgggtaca
    18421 gcgtcttcaa ccaatgaaac aggttggcgt tttgggcggc gggaattgcc cgtgtgagcg
    18481 tctggtagaa atgatgatgg gtattgacca atcctggcag tagaatgtga tccgtgagat
    18541 caagtacttc atcagcagtc gcggggagct ggtcggtggc acctgcctgt tcgatgaaac
    18601 cgtcgcgaat aaagagacca cctcctcgga tctccctgcg gaggtcatcc attgttacca
    18661 ggatatgcgc atttttgacc agaagtgtgc tcataagtca cctcctcaac atgaccaaag
    18721 aaacgaggtg caacccatcg aacgaatttg agtgtaattc gatttaataa ttaagtcaat
    18781 tgtcagacaa ctgagagagc tcaggcgtcc tgaaggctcg ccgctcttaa ggtgttcctg
    18841 agcagcatgg caatggttag aggtcctacc ccgccgggga caggcgtgat cgccctggcg
    18901 acctccgctg cttcatcaaa acacacatct cccaccagac ggtagccgcg gggcttggtg
    18961 gcgtcttcca ctcggttgat gcccacatca atgattaccg ctccgggttt tatccagtct
    19021 tttcttacca tctccgcctt gccaaccgcc gccaccaaaa catccgccga acgcacgaca
    19081 tctggaaggt tcttcgtgcg cgagtggcaa atagtgacag tggcgtttgc tctcaccagc
    19141 aagagggcaa ccggcatacc gacgatattg gacctgccca gcacaaccgc gttaagccct
    19201 tccagcgagg ggaattcctt ttccagcatg tacatcaccc ctgcaggtgt gcagggaacg
    19261 aataacggat cgcgtccttt ttgcgccagc cggccaatat tgatagggtg gaaaccatcc
    19321 acatcctttt cgatggaaat ggcgctgagt atcttttctt catcaagccc tgacggcaga
    19381 ggcagctgta ccagaatgcc attgaccgcc gggtctgcgt tgagctgagt gaccaatctt
    19441 tccacttccg cctggctggc atcccgcggc aactcgtgcc cgaatgactc gatacccact
    19501 tcagcgcaag ccttctgctt ggattttaca tatacctggg atgcggggtt ttcgcctacc
    19561 aacacagttg ccaggccagg ccttgatttg cctgaatcga ggcgctgctg cacccctgct
    19621 gccacctctc ttcgtaattc atcgccgatc gcttttccat ctatgatcgt ggctgtcatt
    19681 ttttcgctcc ttatgtttga tgaattaatt atacctattt taagtttccg agggcgtgat
    19741 ggtaaaatgg agctggtcgt acaacgcaac aatgggagag acacatgagt aaaatttgcg
    19801 tcatcgggac gggttatgtt ggcttggtaa ctggaacctg ttttgctgac cttggaaacg
    19861 aggtcatctg cctggacatc gatcatgaac gcatcgccag ccttaaggcc gggcaaatgc
    19921 ctatttatga acctggtctt gagcagcttg tcaaacaaaa cgtcgccgct gaacgcctgt
    19981 tcttcaccac agattacgct atagcgctga aggacgctga atacgccttt atcgccgtgg
    20041 gaacgccttc tgggaatgac ggcgaagcag atttgcgata cgtgcgctcg gcagccgaag
    20101 ccattgcaga ccatgtaaag aacgccatcc tggtcatcaa taaatccacg gtaccggttg
    20161 gtaccggcga ttgggtggca gacgtgatca atcggcgcag acacggtaaa gcattaaatt
    20221 tcagcgtggt ctcgaaccct gagtttttac gcgaaggctc tgccattaat gactttatga
    20281 atcccgaccg ggtggtgttg ggctcggaag accagtcggc ggcagacagg gtggcccagc
    20341 tctaccagcc attgcgctgt accatcatga ccactgacct gcgaaccgcg gaaatgatca
    20401 aatacgcttc gaacgcgttt cttgcaacgc gcatttcatt tatcaatgaa atcgccaata
    20461 tctgcgaaga attgggcgcg gatgtgcgcg aagttgcccg tggaatgggt ttggataagc
    20521 gcatcggtcc ccagtttctc gatgccgggc tgggctgggg cggctcctgc ttccccaaag
    20581 acgtgaaagc cctggaacac atggccgtgc ttcatggcac tcaaccgcaa ctgctgcagg
    20641 cggtcatgga aatcaaccgt aaccagcggc gccgcgcggt gatgaaatta cgcaagtctc
    20701 ttaaaacgct caacgagaaa acgattggcg tgctcggcat cgcctttaaa cccaacacgg
    20761 acgacatccg cgacgcggcg gcgatcgagc tcattcacct gttgcaaaat gaaggcgctc
    20821 aaatcaaggc ttacgatccg caggcaatgg aaaatgccag tcatgtgctt aaaaatgtgg
    20881 tgctttgtga aaacccttac caggtggcag aaggcgctga tgccctggtg ctggcaaccg
    20941 agtggaacga atttaaacaa ctggatttta agaaactcaa atccattatg aaacaggcag
    21001 tgatcctgga tgggcgcaac ctttgggacg ccagcgcgct gaaagacctg ggattcacct
    21061 acatggggat tgggcgctat catctgcccg gcaaataaaa agtcaaacaa gaggtggctc
    21121 acttgagcca cctcttgttt attacactgt cactggggat cttgctggtg aagctgcaaa
    21181 taggctttta gaacctgccc ggtgtaagaa gtgggcacat cgcacacttc tcgtggcgtg
    21241 ccggtggcga taagttcacc accccgatcg ccaccttcgg gtccgagatc gatgagataa
    21301 tctgcgactt tgatgatatc caggttgtgt tcaatgatga gcaccgaatt accgctatcc
    21361 accaggtgct gcagcacatc aatgagcata tgcacatctg ctgcatgcag ccccactgag
    21421 ggttcatcaa ggacgtacat ggtgcgtccg gttgcccggc gtgaaagttc cttggagagt
    21481 ttcacgcgct gcgcctcccc gccggagagc gtggttcccg gctgcccaat gcggatatac
    21541 cccaggccaa ccttctccaa tgtatggagt ttattggcaa tcgcggggat attcccaaat
    21601 aacttgatac cctcttcaac ggtcagatca agcacatccg caatcgaaag ccccttatac
    21661 ttcacctgca gggtttcccg attgaagcgc gtcccatggc aaacatcaca gggcacgtag
    21721 atatccggta aaaactgcat ttctatgcgc aattgaccct gcccctggca ggcttcgcag
    21781 cgcccgccat gcacattaaa tgagaagcgt ccggctttgt agccgcgcat tttactctca
    21841 ggtaaactgg caaacaggct gcgaatctca tcaaacatgc cggtgtaggt cgcggggttt
    21901 gagcgaggac tgcgcccgat gggtgattgg tcaatattga tgatcttatc cagatattgc
    21961 atgccttcaa tgcggtcata ctctccgggt tgggtgcgcg cgcggttcaa ttcatgcgcc
    22021 aatacgttat acaacaggtc caccatcagg gtcgacttgc ccgaaccgga aacgccggta
    22081 atgcaaacga attgccccag gggtatttcc acctcgaggt tcttgagatt gtttgccctg
    22141 gcgccaacga tcttaagtgt tttgccattg cgcgggtgca atttcttggg cagcggtacc
    22201 ttttttctgc ctgaaagata ggccccggtg aggctgtgcg gatggttgat aatcgcatcc
    22261 actgttccct gcgccaccac ctcgccgcca tgttcccctg cccccggccc caggtccaag
    22321 atccaatcag cggcgcgaat cgtctcttca tcgtgttcga cgaccagcac ggtattcccc
    22381 tgatcgcgca gctgtttgag cgtctcgagt aatttttcgt tatcacgcgc gtgcagccca
    22441 atggaaggtt catccagtac ataaagcacg cccatcaacc ttgaaccaat ctgcgtggcc
    22501 aggcggatgc gctgtgcctc tcctccggaa agcgagccgg caggtcggct gagcgtcagg
    22561 taatccagcc caacatttac cagaaagccc aggcgagaag tgatttcttt gatgatccgt
    22621 tcagcaatag tgaaatcccg gttggagagc accccggagg ttgaaagtga cgcaatgaat
    22681 tcaagtgtgc gattgaccgg ccagttggtg acctccacga tgttgtggtc aagcacggtc
    22741 acagccagcg cttcagggcg aaggcgctcg cctttacagg ttgggcatgg gcggtcactc
    22801 ataaactcgg agatacggtt acggatatat tcagagtgtg tttccctgaa gcgccgttca
    22861 aggttgggaa taatgccttc aaagctcaac tgcatggtgg ttttgcgttc attgcgtcct
    22921 tccatcacca ccggtatgga ttcactgccc gtgccaaaca ggatcttgtc caacttttcc
    22981 tgaggaatcg aacccacagg cgcgtcaagg tcaatagaat accttttggc taccgcttca
    23041 atcatctgcc agtagtaccc cccctggtct cgcggaccgt tccattccaa cgcagcgatg
    23101 gcaccttccc tcatcgatcg ctcggggtca gggatgatca ggtctggatc gatttccagc
    23161 ttgctcccca gcccctggca ttcggggcag gcgccatgag gtgtgttaaa ggaaaacgtg
    23221 cgcggctcaa tttcaggaat gctgatatta tgttccgggc aggcaaggtg ctctgaatag
    23281 aacaggtcaa gcggggtatc accgctcact tcttgcacaa tgaggttacc ctcgcccacc
    23341 ttcagcccgg tttcgattga atcagtcagc ctggaacgaa aagaacgctg ctcttcttca
    23401 tcctcagagc gggtgatcac aatgcggtca accaccgcgt caatattgtg acttttgtaa
    23461 cggtcaagca cgatgtcctg atcaaggttg tacacctgcc catccacgcg tacgcgcaca
    23521 aaaccagcct tacggatctc ttcaaaagct gcctggtaag tgcctttgcg ggcgcgaacc
    23581 accggcgcca ggatgagcag cctggagccc tcgggcagcg cttccacctg atcaactatt
    23641 tcctgggcag attgcttcgc taccactcgc ccgcaaaccg ggcaatgtgg aatgccgata
    23701 cgtgcgaaga gcaggcgcag gtaatcgtac acctcggtca ccgtcccgac tgtggagcga
    23761 ggattgtgcg aggtagcctt ctgatcgatg gaaaccgccg gcgataggcc ttcaatataa
    23821 tccacgtccg gcttttccat ttgcccgagg aattgccggg cataagctga aagcgattcc
    23881 acgtagcgtc gttgcccttc agcaaaaatg gtatcaaatg ccagtgaact tttgccagaa
    23941 cccgagagac cggtaatcac caccaattta tcccgtggta tttccactgt gatattttta
    24001 aggttatgcg cccgcgcgcc aaccactcgt atacaatttt gcgtcattgt ttatccaatc
    24061 gaacatatat tctaattaca ctattttatc acaaaccaac ctttaattat acccttgctg
    24121 tctgcccctt ccgcgcttta atttccaccc tcgtgaatca cagcagaccg tctgcctgct
    24181 tcttgttcaa gagcctcttt ggctcacccg ccgctgctca tcttcagcga aagacagcgc
    24241 tgccagaact gaaaagaaag gtacagcctg gtgtacgcgg ctgcccgtca taaacccagg
    24301 cagaacctca cctgaccagt tgaaccacgc tgcgacccct ctactgtata ataaaacaat
    24361 caatcagtat tgacaggaga gaggcgccat ggcagcaatg aaagaaaaag caaccgaaag
    24421 tgaacggctt cacaagcaag aaatccacgc ctggacgatg tatgactggg cgaactcctc
    24481 ttttgccacc accatgatgg cagcggtctt tccgatctac ttcgccaccc tgggtgcggc
    24541 tggtgaaacc ggtgccatca tgacctcgcg ctggggcttt accaccgcgt ttgctgcgct
    24601 gttggccgct gtaattagtc cgctgctcgg cgcgatggcg gattttcgcg gctccaagaa
    24661 aaagttctta accgtcttca tgctgctggg ggttatcgcc actgccctgc ttttcttccc
    24721 taatcagcct ggttcctggc tgatcgcagc cattttgttc atctttgcca acatcggttt
    24781 tgccggcagc ctggtctact atgacgcgct gctgccacac gtggcccgcg cggatgaaat
    24841 cgaccaggtt tcttctcgcg gttacatgat gggctacttc ggcgggggaa ttttactggc
    24901 tgtcaacctg gtcatgatca tggtcctgcc ggaggttttt ccgcaactgg gtaaagggtt
    24961 gatgacccgc ctgtcatttg tgaccgtggc tttgtggtgg ttcggtttta cattacctct
    25021 gctcttcaaa gtaaaagaac cattacgccg tatcgaagaa ggggaagagg atttgcaccc
    25081 cattcgggcc agcttaatac gcctgaaaaa aacttttttg gatctaaaaa aataccgcga
    25141 cctttcactg ggattgctgg ctttctggat ctacgccaat ggtattggca ccatcatcac
    25201 catggccacc atatacggaa aggaattgaa cttcagcgat accaccttga tcggcacact
    25261 gctgatggtg cagatcctgg cagcgccatt cgccatgctg tttggctggt tggcaaagaa
    25321 gatcggcacc aagaaagcca tctacctcag cctggtcatt tattctctca tcgcggtcgc
    25381 cgggtatttc ctctaccatg aatggcaatt ctgggtgctc ggagccgcag tcgccaccgt
    25441 gcagggcggc agtcaggcgc tttccaggtc aatgatcggt aagatgatgc ccaagagtaa
    25501 atctgctgaa ttctatggct ttttcagcgt gtttgaaaaa ttcgcttcta tcttgggacc
    25561 gttcattttc ggcgtcgtat cacacctgat gaatgaatcc aggctaagta tcgcttcttt
    25621 gatcctgttc ttcggtctcg ggttgttcgt gctgaccaag gtgaacctgg agcgaggggt
    25681 gaaagtggcg tcagatgaag aagccaaatt aataacagtc gaataaatgt ttgaacaaaa
    25741 agaccggaag gaatttgacc accttccggt ctttgattgt gctcgtctac ttcgtcactt
    25801 cgtatagttc caccagcacg ccgccggtgc tctttgggtg aatgaacacc attttccgcc
    25861 cgggcagcac ctgcggttct tcacttaaca gcctgacccc tttttctttt aactgcgcca
    25921 tcataccggc aatatcgtcc acttcgaaac acaggtgatg cataccgccg ccgcgctccg
    25981 ccaggaattt ggcaacgccg ctgtcatcgc tggtgggttt caccagctcc acttcgctct
    26041 cgccgacagg caagaacgcc acctgggctt tctggctggg cacatcttcc aggtggtgaa
    26101 gctgaatgcc catggcatcc tgccagaact tcaatgcctc gtccacatct cccactacaa
    26161 ccgcaacgtg attaattttt ttgattttcg ccatttcgct ctcctgtgat tgttatcccc
    26221 aaacaggggg ttgatactcg ccccaaaccg tgcgcagcac tccgcaaatc tcacccaggg
    26281 tgatctcgtt ctccacgcag gtgacgaaga gcggcattaa attcttgctc gccttcgctg
    26341 ttgattcaag gtgcccaagc aactcgttca ctttcgtctg gtcgcgattg ccgcgcaatt
    26401 cagccagccg aacgcgttgc tgttgctcaa tcgccgggtc aacccgcagc tgctccaggt
    26461 cggaagtatc gttggactgg aaagcattga cgcccaccac cagctgcgct ttgttctcca
    26521 catctttctg gaagcggtaa gcagaatcct gaatctcatt ttgcatgaac cctttttcga
    26581 tcgctgccag cgcgccgccc agatcatcga tctttttaat gtaatccatc acgcgccgct
    26641 caatctcatc tgtgaggtgc tcgatgacat acgagccgcc catcgggtca atggtatcgg
    26701 ccacgccgga ttcgtaagcg atgatctgtt gggtgcgcag cgcggtgcgc acagattttt
    26761 cagtgggcag ccaaagcgct tcatctttgg aattggtgtg cagcgactgc gtgccgccca
    26821 atactgccgc cagcgcctgg attgccacac gcacaatgtt attttcaggt tgctgcgcgg
    26881 tgagcgttga accgcctgtc tgcgtgtgaa agcgcagcat ccaggattta ggatctgccg
    26941 cgccgaaacg ttcgcgcatg atacgcgccc ataagcgcct cgccgccctg aatttggcaa
    27001 tctcctccag aaagtcattg tgcgcggcaa agaaaaagga aagctgcgaa gcgaactggt
    27061 ccacttgctg gcccgtgtct atggctgcct gcacgtaagc gatggcgttt gccagggtga
    27121 aagccacctc ttgcacggcg gttgagccag cctcgcggat atgatagccg gagatgctga
    27181 tagtgttcca attgggcact tcagattcac agtacttgaa aatatcggta attaagcgca
    27241 gcgagggctt gggcggaaag atatacgtgc cgcgcgccac atattccttg agaatgtcat
    27301 tttggatggt gccgcgaagc aggcggggag aaacaccctg ctttttaccc atggcgatgt
    27361 acatcgccag caaaatggct gctggtgaat taatggtcat gctggttgaa accttttcca
    27421 gcgggatgcc ggcgaacagg gtctccatat cgcgcagcga agaaatgctg acccccacct
    27481 tgcccacctc gcccatcgcc atcgaatcat ccgcgtcgta accaatctgc gtgggcagat
    27541 cgaaagccac ggaaaggccg gtctgcccct gctccagcag gtaacggtag cgcctgttgg
    27601 attcttccgc cgaggcaaac ccggcgtact gccgcatggt ccagaagcgg ctgcggtaca
    27661 tggtgggctg gacgccgcgg gtgaaagggt attcacccgg gaaacccagc tgttccatgt
    27721 aatcagcgct accgggtatg gctgcgcgcg ggatgggtat tccggatggc gtggtgaatt
    27781 catcttttct ctctttaaat ttttctacca caggctttac cacttcttca tcccaacgtt
    27841 ttttttcgct ttcaaggttc atgttttcgc ctctgtaaat gttgatgagc catctgaatt
    27901 gagtatacac gaatccaaaa tggatgtgtg agaaattatg ataaactgaa ataaaaatcc
    27961 attagcgcgt caatccatgt cgaatttcct tcaactcatc gccgagatcg ccatcatcct
    28021 gctcgccgcc aaagccgcgg gttatttaag cgtacgccta aagcaacctt cggtatttgg
    28081 cgaactgctc gtgggcgtgc tgcttgggcc ttcgttgttg gatttcacca acctgggctt
    28141 tgtcacctct acccacctga gcgaaaccat tcacgaactg ggcgagatcg gcgtgctgct
    28201 gctgatgttc ctggcagggc tggagctgaa catcaaagac ctcacccgca acacacgcgt
    28261 ggcattgctg gcaggcatgc ttggagtggt ctttccggtg gggtttggca tcgttttcgg
    28321 cgaattattg gatattccct tccgtcaggc gctgttctta gggctcacgc ttggcgccac
    28381 atccgtttcc atttccgccc aggtgctgat cgaactgcag gcgctgcgca cgcgggtggg
    28441 gctcgccctt ttaggcgccg cggtttttga cgacatcctg gtcatcctga ttctctcttc
    28501 tgttttagcc ttcctgtctg gcagtggcgg gttcagggaa attatgtggg tttttacgaa
    28561 gatggtcatc tttttgatgc tggctgccgc ggtggggttg tggctgctgc cacgcgtggt
    28621 gcgccttaca cgatctctta aaatcagcca gggactcacc accctggccc tggtggtgct
    28681 gttaggttat gggctggcag cagagctgat gggcggcatg gccgcaatca ccggcacatt
    28741 cctcgcgggg ctgatgttcg cccgaacacc tgaaaaaggc accatcgaaa cgaacatgca
    28801 tcccatcgct tacgctttct ttgtgcccat ctttttcgtc agcatcggtt tgaatgtgaa
    28861 cctgcgtgaa ctgaacgctg attccatctg gttattcctg ggcatttgcg gtatcgccat
    28921 cgcgggtaag gtcatcggct ccgg
//
LOCUS       DAOTLW010000025        28434 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_1955_length_28434_cov_10.922971, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000025 DAOTLW010000000
VERSION     DAOTLW010000025.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 28434)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 28434)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28434
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_1955_length_28434_cov_10.922971"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..666
                     /locus_tag="P5516_05425"
     CDS             <1..666
                     /locus_tag="P5516_05425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91914.1"
                     /translation="WFKQRAWQYLALLFSIPLLMLPSILALAFPIENPSLSRAGGSLI
                     PILIVAAIALEGLLRGLWHTSRGWFGRALVVALGVGLLLVSAQQNYDLVFRQYRLQYQ
                     GATWNSGQMGAIARAYIHSIGSTDSVYVVAVAHWVDTRLVAMNAGVFDRDYAIWADDL
                     ELTLGQAGPKLFFVKADDTAGMSRLTALYPEGFGNYHSGMADGRDFYTYLVSPALSGG
                     FQQ"
     gene            complement(644..1405)
                     /locus_tag="P5516_05430"
     CDS             complement(644..1405)
                     /locus_tag="P5516_05430"
                     /inference="COORDINATES: protein motif:HMM:NF019322.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HRT91915.1"
                     /translation="MSRTIDPFTAVPKYFQLASILRQNIEDGEWAPRSPIPSERQLEV
                     LYNISRTTIREAINSLVQQGYLYREHGRGTFVSPQKLQKGWMELTSFSEDLVKRGLVP
                     GQIIRSLQLMQPPQRVLQRLELPAGSLVHRIERVRLADGTPIGLQTSYLSLAPGQEIT
                     MQEMEKAGSLYRILSEKFHIIPTEADETLEVTLATPEEAGLLQVATGAPLLLNERVLF
                     SQERTPVEYVKILYRGDRYQYFVHLKRQAIAENPR"
     assembly_gap    1459..1558
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1559..2008
                     /gene="rpiB"
                     /locus_tag="P5516_05435"
     CDS             <1559..2008
                     /gene="rpiB"
                     /locus_tag="P5516_05435"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF004051.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRT91916.1"
                     /translation="IGTDHGGVELKKVLIQEISHMGFKIMDCGTNTSDPVDYPDIALA
                     VAQQVAQGAAWRGVVIDGAGIGSCMAANKVPGVRAALCYDYATAVNSREHNDANVLTL
                     GAGLIGVNLAKQVLKTWLTTEFGGDRHARRVDKIMEIEKKYLSGKTA"
     gene            2005..2931
                     /gene="deoC"
                     /locus_tag="P5516_05440"
     CDS             2005..2931
                     /gene="deoC"
                     /locus_tag="P5516_05440"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HRT91917.1"
                     /translation="MTPDIKTVETLVEIITREVLLAMIEQEERQKNPPGATCVVEVAN
                     GVKVKTCFDNAGHVISAGAERLTSTVGVIPGDSALAGMIDHTLLKPDATSDKIAQLCF
                     EAKKHHFASVCVNPTHVKLCADLLKDSDVKVCTVIGFPLGATSPEVKAFETRNALENG
                     ATEIDMVLNIGALKAGDTNLAARDIRGVVEVAHAANALVKVIIETALLTDEEKVIACL
                     LAKEAGADYVKTSTGFSGGGATVHDVALMRRTVGPSLGVKASGGIHTHEEAEALVAAG
                     ATRIGASAGVKIIRADQSETKPQIAAPEGKAY"
     gene            2941..3231
                     /locus_tag="P5516_05445"
     CDS             2941..3231
                     /locus_tag="P5516_05445"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRT91918.1"
                     /translation="MLIARVIGTTVSTVKDEKLTGRKLLIVRQTDEHGEAVGKPFVAV
                     DTVDAGVGDLVLTAAGSSARQTAITKDTPVDTVIMAMIDTLEVDGVVTFRKS"
     gene            3239..>4464
                     /locus_tag="P5516_05450"
     CDS             3239..>4464
                     /locus_tag="P5516_05450"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRT91919.1"
                     /translation="MPDLDQDLLSIQEARNLATRAFEAWKTWAKASQQDVDRVCAAMA
                     AAAFNAADRLGQMAHEETGYGIPAHKRLKNEFAARNVWESIKDIKTAGVIHHDPEKRI
                     YQIAWPMGVIAGLTPSTNPTSTVIYKILIAVKARNAIVIAPHPSAARCSYEAAALMVK
                     AAEENGAPPGLIACMQYVSLAGTQELMGHKYTALILATGGTPMVRAAHSTGKPAYGVG
                     PGNVPAYVDRSADLEKAARYITASKAFDCSTICATEQAVVADRPIARSLQRLMEQEGA
                     YFVDKAQTDLLRRLLFNSEGVMVPATVGKPAPYLAALAGFNVPAGTRVLVTRLNRVGK
                     EEALSREKLTTVLGWYEADGWEAGCERCIELIQFGGRGHSLVIHATDEKVIMAFGLEK
                     PVFRIAVNTMSTLGAIG"
     assembly_gap    4465..4564
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4565..5299
                     /gene="eutJ"
                     /locus_tag="P5516_05455"
     CDS             <4565..5299
                     /gene="eutJ"
                     /locus_tag="P5516_05455"
                     /inference="COORDINATES: protein motif:HMM:NF011660.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ethanolamine utilization protein EutJ"
                     /protein_id="HRT91920.1"
                     /translation="AYTVLVVLDEAGNPLAGEYQFAQVVRDGLVVDFFGAVNLLKTLK
                     DKAERRIGRLLTRAASGYPPGVPRAEVRATTHVVEAAGMVCERLVAEPEAANQVLGIH
                     NGVVVDVGGGTTGIAVLENGKVVYTADEATGGTHFSLVIAGATGLSFDQAEALKKTTS
                     EQAGLFPLVRPVMEKVGTIIMRHLRGSAPESITLVGGTALFPGMAEVVQQVTGIPTRV
                     PAHPMFITPLGIALSDNDTDPRRQDG"
     gene            5292..5918
                     /locus_tag="P5516_05460"
     CDS             5292..5918
                     /locus_tag="P5516_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91921.1"
                     /translation="MAEDFSLRCYCFIDRMQAQYAAFVGTITQGDLPVEGMAALYVEM
                     APGNEVFRVVDIAVKATEAKPGAQIVEREFGMFEVHSLNQAAVIEAGQVVLDRLGLNK
                     EDRIKPEVVSTQIITRIDPYQSQLINRFRRGSMLVPGETMLVLECAPAAYINYAVNEA
                     EKKANIKIIHVSSVGRFGRMWLSGSEAEIINARNAATSALESLEGIKG"
     gene            5943..6296
                     /locus_tag="P5516_05465"
     CDS             5943..6296
                     /locus_tag="P5516_05465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91922.1"
                     /translation="MPKKFFTERDIEEMAARGEMFLEVSDEVVLTELAYEKAQCLGVR
                     LHQTNSLPPAAPIRPYLAQAVNSCPECSTSQKPAEAELRKRIRDAVAARLGTQVDPAL
                     LETIITRVLNNVGMK"
     gene            6300..6620
                     /locus_tag="P5516_05470"
     CDS             6300..6620
                     /locus_tag="P5516_05470"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRT91923.1"
                     /translation="MLFGRVHGTAVCTIRVPGTEGLKFLVVQPLNRKLEPLGPLQVAV
                     DVVQAGVGDLCVMVRSREASLVMPEVKFVPVDLALVGIVDELSVKPDGVFDLVMPKGT
                     CEYT"
     gene            6628..6897
                     /locus_tag="P5516_05475"
     CDS             6628..6897
                     /locus_tag="P5516_05475"
                     /inference="COORDINATES: protein motif:HMM:NF015289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EutN/CcmL family microcompartment protein"
                     /protein_id="HRT91924.1"
                     /translation="MILAKVVGTVVTTISHPHYKNRRLLVVQPLVTEGESSEEDFIAI
                     DNTHAGIGDTVLVNREGNGARQVLNNPDACIISVIVGIVDSVTIN"
     gene            complement(7160..7570)
                     /locus_tag="P5516_05480"
     CDS             complement(7160..7570)
                     /locus_tag="P5516_05480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HRT91925.1"
                     /translation="MKVRVVSFILMVLLLTSLNACAKAAVHTPTAAVEEPVEVETSTP
                     TEVAVDVTSATAQGQTDAPGPKVLPPGAPVIDGKELLDSRCASCHTLAKVTDTTATAE
                     EWAAIVTRMSAKGANLTEEERSILTLYLAENYKK"
     gene            complement(7575..7982)
                     /locus_tag="P5516_05485"
     CDS             complement(7575..7982)
                     /locus_tag="P5516_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91926.1"
                     /translation="MNSKISTELIVVIVAIVIFYLRVAMLRGQKKRYERDLALKRRKV
                     KGRSKGSPLPQQPKGTPPFTVRSWVLVGISMLLMLAGVVAYNKFYFLGMQLVPDPAFV
                     EAYAKFWYILVSAGVILLAFTVTIRKPIEDSVE"
     gene            complement(7979..8470)
                     /locus_tag="P5516_05490"
     CDS             complement(7979..8470)
                     /locus_tag="P5516_05490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91927.1"
                     /translation="MDKKKSHQWNFAFDAMPALFHSQTDGFVKYLDKDGVKFLKFYWD
                     LLGDKLPEEKRISAAGLTFTKEVIDSKNHLIIITLPSPKEDGDPYFIGLVPKPERRFA
                     IVRFYNSTMFVLLRKDDTDHANRTSFGEVTPKGNYHERGIGLKPTQQDFKRVIKNRLE
                     RKK"
     gene            complement(8474..9382)
                     /locus_tag="P5516_05495"
     CDS             complement(8474..9382)
                     /locus_tag="P5516_05495"
                     /inference="COORDINATES: protein motif:HMM:NF017206.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HRT91928.1"
                     /translation="MILLTGAAGKTGKTILNRLIPHGVHVRSLVRNEQQAQEIRQLGS
                     TDVAIADLRDQQALSQALDGIHKIYFICPNMTPDEVEIGRSLITLAKQKGIRRFVYHS
                     VLHPQVEEMPHHWQKMRMEECLFKSGLDFTILQPCAYMQNILANWTSIIDEGIYPVPY
                     DTSARISVVDLEDVAAAAEVVLTQMNHSHAIYELAGPQALSQDEMAEVLTQEIGRPVK
                     AVAIDRSDWAVNAQRSGLDENSIDTLLKMFEYYEHYGFQGNPNVLNSLLKKPATKFVD
                     FVKRTHESRLRYGSNGQNGQNHHHRK"
     gene            complement(9494..10912)
                     /locus_tag="P5516_05500"
     CDS             complement(9494..10912)
                     /locus_tag="P5516_05500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="solute carrier family 23 protein"
                     /protein_id="HRT91929.1"
                     /translation="MATTSKVLGYLPQDTPPFGRMLLLGFQHVLTMFPATVLCALLMH
                     FDVSTVLTITGLGTVVALLGSKFAINTYIPLYYGSSFSYIAAVASIIGVLEPDLAFGA
                     PAKPSTIAVATAGFIVTGILNILVGLLIRATGGKKGLDKVLPAEITGSVAVTIGIGLA
                     FTALTMASGTCCGVEPNMPPTLKWWLAAALTFLSAVIFSVYLQGKGFIGMLPILLAMI
                     FGYIVAIPIGLVDFSTIGQSGLLTVPKIVFPVFNNALTATAMIGVGVMAIATIPESTA
                     HLYQISLYVDHLAEEMGREKPYFAKHIGLNLMLDGLNDLVNGLFGSTAGTNYGENNSL
                     MVITRNYSGPALITAGTIAVVLGFIGPLRDAIYSIPTAVTGGLAIYLYGVIGVQGIAL
                     MMAEKVDLYDPGKLAIVALILVVGIGGNIGYGGNLPIPLLRGVFPFGWPAIAAGAVFG
                     MIVNLLFLVWKPPKVRSTDVLE"
     gene            complement(11163..11315)
                     /locus_tag="P5516_05505"
     CDS             complement(11163..11315)
                     /locus_tag="P5516_05505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91930.1"
                     /translation="MAEELKEAATSAEKPAEPKKKFMEVAEYRMILYVVPVALVLLVL
                     AIILGK"
     gene            complement(11300..11755)
                     /locus_tag="P5516_05510"
     CDS             complement(11300..11755)
                     /locus_tag="P5516_05510"
                     /inference="COORDINATES: protein motif:HMM:NF017999.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon monoxide dehydrogenase subunit G"
                     /protein_id="HRT91931.1"
                     /translation="MKVEGEHLFKAPREVVYEMFNDPDALGAAVPGMQKMIKLDESHY
                     EAVLGIRVGPVSANFSGNLSLTDEVPPESVTLVIEGKGGAGFVKGVGYVKFEDLGDET
                     TLLKYTGEANIGGTLASVGQRMIDSVAKSMFKTVFEKLDKILEERWQKN"
     gene            complement(11818..14172)
                     /locus_tag="P5516_05515"
     CDS             complement(11818..14172)
                     /locus_tag="P5516_05515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRT91932.1"
                     /translation="MTGKYFGNPIKRLEDKQLLTGNAKYIDDIEVPGQLHAAFLRSDF
                     AHARILKIDVSEALKRPGVVAVYTAEDFGDFWKPGPLQVPPPTAIPGSKFNARTLVPI
                     AKDKIRYSGEVLAVVIAESRYIAEDAFDDIIVDVESLPAVVDLEKAWDDSSVLVHDDL
                     PSNMAALVTQEVGNYEEAKKKADVVVARRMLIEHVAGAAMENRGFLASWDDQNQVMTI
                     WANTQSPLTVRGSVSRALGLAESQVHVITPAIGGGFGPKVMTSLTDDVLLPWISKQLK
                     RPIKWVEDRRENFLATTSERDQVHHCEIALKKDGTILGFKDVFYHNTGAYDSYGMTVP
                     LNTQTHIISNYRVPNFYTQIRMVFTNQMVVTPVRGAGRSEGVFAMERMLDFAAKEMGL
                     SPIEIRRKNLLRADELPLATGITGQDFVKNTLDSGDYLGNFEKCLQAIEYDKFIKETQ
                     PKAQKEGKRLGIGVVAFTEGTAVGPYEGCKVTVSTSGKVTMTTGYSSQGQAHYTVFAQ
                     IIADQLNVKVEDVKVITGDTNHFAWGAGTFASRGATLVGTAALLASQKVRAKIFSTAS
                     KALGVPEDQVELGEGSVSIKGKPDSAIPLGTLAAMANPMRGVIEPGVEPGLEATAYYG
                     PPHGSTGCGAMGVILSIDEETCKPVLEKMVIVDDCGTMINPLIVDGQLQGGISMGIGN
                     TLYERLVYDENGQLLTASFMDYLMPLATDMPKKLEIIHNHAESSLKLNPLGIKGVGEA
                     GAIPPGPAIVQAIEDAFADKGVEILETNLSPSSIYGYLKKVEHK"
     gene            complement(14187..14669)
                     /locus_tag="P5516_05520"
     CDS             complement(14187..14669)
                     /locus_tag="P5516_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRT91933.1"
                     /translation="MKKLFPITVTVNGKVYERQVEARLLLSDFLRHDLGLLGTHVGCE
                     HGICGACTILFDGEAVRSCLIFAVQANGHEIRTVESLGTPENLHPLQQSFTEKHALQC
                     GFCTPGFLMTLVPFLEENPTPTEDEIREAIDGNICRCTGYEKIVEAVHDAAEKMSQKK
                     "
     gene            complement(14669..15553)
                     /locus_tag="P5516_05525"
     CDS             complement(14669..15553)
                     /locus_tag="P5516_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRT91934.1"
                     /translation="MKPVNFDYYAPSSVDEALDTLAELGYDGKVLAGGQSLIAAMNFR
                     MARPSALVDLNNVAELSYIKPTADGGLAIGTMTRVSTVEHNQDVIQRFPLLVEVAKFI
                     AHPQIRRRGTFGGAIAHADPAGQLPSISMVMNMQALIKGKGTERWVAAPDLIIGPFMT
                     VIEPNEMLAELVIKPHPPRTGAKYVQVSRQSGGYAQSAVASIVTLDENEICKDVHMVL
                     MSVAEVPILSEKAREILIGNKPSKDAIEAVAEAASTSEIDPASDLHASADYRRSITRV
                     LIVRSLTEAFERAKRGGK"
     gene            complement(15691..17358)
                     /locus_tag="P5516_05530"
     CDS             complement(15691..17358)
                     /locus_tag="P5516_05530"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRT91935.1"
                     /translation="MNREDFIKMLTDAELYDLTQDCSIFTPPHPHEKGLEVHFFKRVT
                     GAYGGGQGANGQILNWSNTVGTHLVGERAFNSGGRAIADIPLTDLCGPGVVADISDLV
                     SDYSIYTPEMITSKVEVKKGDILIVNTGYHKYSWDQPAVRNKTAQGGVESMEFGFLVR
                     HPGPSMDFYKWAMDMELKVIGVDCGSAEHPMNTPIRRWHEDHFKLAEAKLLKETGKTW
                     DETFPQGPYYQLTHIDMPKKHLLLAEAIVGDIDKLKNKRAWISIMPIPYMEVETAWSR
                     VFAWTAPKHMSEEEFFAAMKKSEMIDMTVPFSVQTPQWLNYVPLTVTYTKRVGGQYFG
                     MGRNGSICNASIHLGTHMDGEKHFFPNGRTIGQVPLKDWVGPGVIADISKLVSDSSVY
                     TPQMIMDQVEVREGDILVIKTGWNKYGWNSKDSDEFRYMVKHPGPSPDFSEWAIKMKI
                     KWIGVDAVSADHPMNTIMRIWHPKTFAEANAKLIKDFGKDWDELYPLKDFYQDMHLNL
                     FPHTIVHAENLAGQLATAKSGRYYIGCYLQKAMEAESMWGRFVAFKE"
     gene            complement(17387..18634)
                     /locus_tag="P5516_05535"
     CDS             complement(17387..18634)
                     /locus_tag="P5516_05535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1116 domain-containing protein"
                     /protein_id="HRT91936.1"
                     /translation="MVDIEKANALAVEKFMEARPVAITLAKALEIVPGMHENMLLHAG
                     PPVTFERMSGPTKGAMIGALLFEGKAKNPEEAEKLLKSGKIEFSPCHEHMCAAPMAGV
                     ISPSQLVYVIENQTHGIKCYSNLNEGRGKVLRMGAYSEDVLEKLRWMEAVLGPTIKTA
                     LEAMGGIDVRAILAKALHSGDDGHNRLDTASLLWTTQLAPYIAKTAKNTETAFEVTKF
                     LGENALSILNPVMAGCKTMTAAGHNIEGSTIVTIMSRNGTDWGIKVSGLGDKWFTAPS
                     PVVKALYFPGFTEKDACPDIGDSVITETAGIGGFAMATAPALVTFVGGEPRDAINTTL
                     DMYEITEGEHKQFTIPFLDFRGTPTGVDIRKVVEKQITPRVNTGVAHKDPGVGQVGAG
                     VVSAPMKCFEDALIAFADKYGYN"
     gene            complement(18670..20406)
                     /gene="fdrA"
                     /locus_tag="P5516_05540"
     CDS             complement(18670..20406)
                     /gene="fdrA"
                     /locus_tag="P5516_05540"
                     /inference="COORDINATES: protein motif:HMM:NF004760.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase FdrA"
                     /protein_id="HRT91937.1"
                     /translation="MATFKTEVRSGVYYDSAKLMSLQRSLAGLPGVLDAGVVMGTDSN
                     KELLAHIGLDTPEVKATKPDDLAIVIKAENEKAALDALAQVDGLLAARKGGSEQEYQP
                     RSIESATDMLPDAEWVIVSVAGRYATAVTREALRLRKHVHLFSDNVSVENEIELKKEA
                     RDLGLLVMGPDCGTAMVDGYGLAFANKIRKGPIGIVAAAGTGLQQVATRIHQLNSGIT
                     FGIGTGGRDLKAEVGAITFLMALDVLARDDDTKVIVLTSKPPSPKVAEEVLKVARKAG
                     KPVVVNFIARPITTTRDGNLFFATGLDDAARLAVELAHNPPKLVSDADLPIKKFTAKQ
                     KYLRGLFSGGTLAYEAQHILYGYVPRVLANSPINKEFQLPDAFVSQENCIVDLGEDEF
                     TQGRLHPMMDNELRIRRLYEEAKDPEVAVILLDVVIGYGSHPDPASEIAPAIAKCIAD
                     AKKDGRHLEVVCVVCGTDEDPQNLNNQMAQLKAAGALVDTSNEVICRYVGRILRALAE
                     RDEPKAHALGKPVDLEKIKKPLAAINVGLESFAANLKDQGAPYVQVDWKPPAGGNEKL
                     MNILQKMKSPRK"
     gene            complement(20709..21434)
                     /locus_tag="P5516_05545"
     CDS             complement(20709..21434)
                     /locus_tag="P5516_05545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91938.1"
                     /translation="MKIEFPTPVSTEEMKIDLREIEGILQGAPMDMPPIPEATVTLKD
                     GRIVYIRGIKDSDIDPMLGYLEKVMKVDKDFYDIVGVRVYGEVLALRRKRLKDPFTMV
                     GLVDGEWLGFANGRAWDKDIAISLHTLTFSRKARLGWPMYYAKTYYAMELLKVKEWWS
                     TFESYNGWRMAGLSMAQPTKPWPEYQHELGGAKVFYLNQDMWHSRVKDYVREIVGQDM
                     DFNVTDEVRKANENFRVPETVDL"
     gene            21670..21987
                     /locus_tag="P5516_05550"
     CDS             21670..21987
                     /locus_tag="P5516_05550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91939.1"
                     /translation="MDKETQMLTIFFRHDQSKSLDEIQAHLRANGFWRDFPPEGMEIV
                     SWVVMMGIGHVVTLRLPQSRLRELNMIVEKKAWKAFTTEFYPTYDFLPHYQQFHAEAA
                     ARD"
     gene            complement(22032..23417)
                     /locus_tag="P5516_05555"
     CDS             complement(22032..23417)
                     /locus_tag="P5516_05555"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRT91940.1"
                     /translation="MSELIDHLLAKAREAQKVIEFWPQEKVDEMILAVGWEAYKRETA
                     ETVARLAVEETGLGVYEDKVLKHQKKTIGVLRDLQGEKTVGVIERVPEKGLVKIAKPM
                     GVIGAITPMTNSSSTMPCNGLMILKARNAVIFAPHPRAKKTCAVTCEEMRKGLAKVGA
                     PLDLVQYIEEPSIELTQELMSKVDLVLATGGAGVVKPAYSSGKPAYGVGAGNATVVVD
                     ETADLPVTAAKICKGKIFDNATSCSAENNIVIQESIFDDLLEELKKTGGHLVTGEDRA
                     KLKAAMWPDGVHLSGKIVCKSVKFIAEEAGIHVPDGTKFIMVLGEHIGPEDMFSAEKL
                     SPVLTLWKYKTFDQAVQMVIDITGFSGYGHSCGIHSVNEEHILELATKAKVSRMMVRQ
                     AQSVGNSGDWENGMPFSMSLGCGTWGGNITSENITVKHFLNVTWVSYPIPAEIPDPEV
                     IFAPHFAKYGK"
     gene            complement(23454..24725)
                     /locus_tag="P5516_05560"
     CDS             complement(23454..24725)
                     /locus_tag="P5516_05560"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRT91941.1"
                     /translation="MVNYQENLKHLSPVWTHLTDIVVDHAEGCYLYATDGRKMLDFTC
                     GIGVTNTGHCHPKVVEAIRQQAGLLIHGQVNLVVHKPLLELVDELRTVLPSTLDGFFF
                     SNSGAEAVEGALKLARHATGRPNVIVVQGSFHGRTNATMAMTTSKNSYKIGYQPLMPG
                     VYVTPFPYALRYGWDEEKASQWCLDELELLLMTQLAPQETAAIFIEPIIGEGGYLVPP
                     KTFMKGLRELTQKHGILLVADEVQSGFGRTGKWFGYQHFEIEPDIMTVAKGIASGMPI
                     SGVISRLELMSKWIPGSHGGTYGGNAVAAAAAVATIRTIKEEKIIENVVTRGAQLKAG
                     LAHLQEKYPQIADVRGLGLMVGTEFRDAHGKPDKKTTKAVQAECLSRQMMLLTAGPWD
                     NTIRWIPPLVVTESQMNEALNIFESALAAVI"
     gene            complement(24944..26149)
                     /locus_tag="P5516_05565"
     CDS             complement(24944..26149)
                     /locus_tag="P5516_05565"
                     /inference="COORDINATES: protein motif:HMM:NF027964.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HRT91942.1"
                     /translation="MKVLLVGVGGVGEAIAIMAAKRPWLEKMVLADFNKERALEVQAK
                     IKDNERFPVEFVDARNRGMIEELAKKHKVDLIMNACDPSFNETIFDAAYNVGTNYMDM
                     AMTLSHPHPETPYTKTGIKLGDYQFARADKWKEKGILALLGLGVEPGMADVFAKYAAT
                     HLFDEIDEVNVRDGANLIVKGYDFAPNFSIWTTIEECLNPPVIWEKGKGWFTTEPFSE
                     KEVFDFPEGIGPNECVNVEHEEVLLVPRYIDCKRVTFKYGLGDEFIGILQTLHLLGLD
                     NKEPIKVGKVEVAPRDVVAACLPDPAHLGPLMEGQTCAGTWVKGKKDGKERNVYIYQV
                     ADNKESMDKFDCQVVVTQTAFNAVIGMELLAEGIWKATGVEGPEFFDPLPFMDRMAGM
                     GFPWGMKEW"
     gene            complement(26175..27776)
                     /locus_tag="P5516_05570"
     CDS             complement(26175..27776)
                     /locus_tag="P5516_05570"
                     /inference="COORDINATES: protein motif:HMM:NF012544.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HRT91943.1"
                     /translation="MAPEIDKATLVRSEKQKLKRELTILPLFALIYFTVCGGAFGAEP
                     MVGLSGPGLALLLMVITPLVFSIPNMLMVREMQSMMPVEGGYYHWVKQAFGPFIGFMS
                     GWMNWVVSWVDVSIYPVWTAWYLSYFIPALSDGATIGGIHFSSDLLSWLVSAALIITI
                     SLLNVRGAKLSGLTASWLGVAMIIPIIIMSGFGIYAWIVNGTPSNIPFLPGGAEVSGR
                     NLLGALSVGIYVAMWNFMGWELPTAAGDEIKNPKKTYPRAMVLVLIATILSYSVPTVA
                     GLYGGAGEDGQYLAWGLEESAPGTGIGPILNDYGIADEKMLTWGIDPGSDAGWEFPTI
                     AHRIGDKVAGKDSPLSYFLGAIVGISAIFSMVGLFIGNGLGGTRVPYAMAEDGMMPTW
                     LNKVHPKYGTPWVSILICGAFYLIFSTSAFQNLVVIDVFLNMLVLMAEIFALVVLRYK
                     KPDIPRKRVPGGIWGLIYIVVAPLTIIIIAIVSQIIDYGWQGAVGLALIAMAIGAVLY
                     FPIRKWVKKDIPDVDPYVLEAAEEA"
     gene            complement(27878..>28434)
                     /locus_tag="P5516_05575"
     CDS             complement(27878..>28434)
                     /locus_tag="P5516_05575"
                     /inference="COORDINATES: protein motif:HMM:NF013224.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HRT91944.1"
                     /translation="VFQKHLYFRKVYLYLLYISAIFRSKLIKIDAIDKKISDLLLQDG
                     RMSCAQIAKRIGGEVSERAVRYRINRLVENGIISVRGNVNAENIGLVVCADVFIEVEP
                     SQIKTIAMKLAEYESISYIAYATGDTDISIQLFTPDNSTMFDFVTEVIGKIPGVRKTH
                     ISFVPIKIKDDHVWPIPSFIVTQD"
BASE COUNT         6305 a         7313 c         7836 g         6780 t
ORIGIN      
        1 tggtttaagc aacgcgcctg gcagtacctg gcgctgctgt tctctatccc gctgctgatg
       61 ttgccgtcga tactggcgct ggctttcccg atcgaaaacc catccttaag ccgggcggga
      121 ggctcgctta tccccatttt gatcgtggct gccatagccc tggaaggctt gttgaggggt
      181 ctatggcata catccagggg ctggttcggc agggcgttgg tggtggcact gggggtgggt
      241 ctattgctgg tttcagccca gcagaactat gaccttgttt ttcgccagta tcgcttgcag
      301 tatcaaggcg ccacctggaa ttcagggcag atgggcgcca ttgcgcgcgc gtatatccat
      361 tccattggca gcaccgatag tgtgtacgtg gtggcggttg cccattgggt ggatacgcgc
      421 ctggtggcga tgaacgcggg agtttttgac cgtgattacg ccatctgggc ggatgatctt
      481 gaactcaccc tggggcaggc gggccctaaa ctcttctttg tgaaggcgga tgacaccgcc
      541 gggatgagcc ggctaaccgc cctctacccg gaggggtttg ggaattatca ttctggaatg
      601 gcggatggcc gggactttta cacttatctg gtatcgccgg cgcttagcgg gggttttcag
      661 caatagcctg gcgctttaag tgcacaaagt attgataccg gtcgccacgg tacaggatct
      721 tgacgtattc gactggcgtg cgctcctgcg agaacagcac gcgttcgttg agcagcagcg
      781 gcgcgccggt ggccacctgc agcagcccgg cttcttcagg ggtggcgagg gtaacttcca
      841 gtgtttcatc tgcttccgtg gggatgatgt gaaatttctc gctcaggatg cggtagagtg
      901 agcccgcctt ttccatctcc tgcatggtga tctcctgccc gggagccagc gacagatagg
      961 atgtttgcag gccgatgggg gtgccgtcag ccaggcgaac gcgttcaata cggtggacca
     1021 ggctgccggc tggaagttcc aggcgctgca gcacgcgttg tggtggctgc ataagctgaa
     1081 ggctgcgaat tatctgcccg ggtaccaggc cgcgcttgac caggtcttct gaaaagctgg
     1141 tcagctccat ccagcccttc tgcaacttct gtggtgagac gaaagtgcca cgcccgtgtt
     1201 cgcggtaaag atacccctgc tgaaccaggg agttgatggc ttcgcggatg gtggtgcggc
     1261 tgatattgta aagcacttcc agctggcgct ctgaagggat gggggagcgc ggtgcccatt
     1321 cgccatcttc aatattttga cgtaaaatag aagcgagttg aaaatacttg ggcacagcgg
     1381 taaatggatc gatcgttctt gacatttgtt cttaaagtgg tatgatggaa ataactggta
     1441 atgtggtcac taccagtann nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnat
     1561 tggaaccgat catggcggcg tggagttaaa aaaggttctc atccaggaaa tttcacacat
     1621 gggttttaaa attatggatt gcggcacgaa cacgagcgac cctgtggatt acccggatat
     1681 tgccctggca gtggcgcagc aggttgcgca aggcgcagcc tggcgtggcg tggtgattga
     1741 tggcgcgggc attggttctt gcatggcggc caataaagta ccgggggtga gagcggcttt
     1801 gtgctatgat tacgctaccg cggtgaacag ccgcgagcat aatgacgcca acgtgctcac
     1861 gcttggcgcc gggttgatcg gtgtcaacct tgccaaacag gtgctcaaaa cctggctgac
     1921 cactgagttc ggcggcgaca ggcacgccag acgtgtggat aagatcatgg agatcgagaa
     1981 gaaatatctt agcgggaaaa cagcatgaca cctgatatca agaccgttga aaccctggtt
     2041 gagatcatca cccgcgaagt gctgctggcg atgatcgaac aggaggagag acaaaaaaat
     2101 cctcctggcg ccacctgcgt ggtggaggtc gccaacgggg taaaagtgaa aacctgtttt
     2161 gataatgccg ggcatgtgat cagcgctggc gccgaacgcc ttacctcgac ggtcggtgtc
     2221 atccctgggg atagcgcgct ggcgggaatg atcgatcata ccctgcttaa accggatgcc
     2281 accagcgaca agatcgcgca gttgtgtttt gaagccaaaa agcatcattt cgcctccgtg
     2341 tgtgtcaacc ccacgcatgt aaaactatgc gctgacctgc taaaagatag cgatgtgaag
     2401 gtgtgcacag tcatcggttt tccgctgggc gcgacctcac ccgaggtcaa agcatttgaa
     2461 acgcgtaacg cgctggaaaa tggcgcgacc gagatcgata tggtgttgaa cataggcgcg
     2521 ttaaaagcag gcgataccaa cctggctgcc agagatatac gcggtgtggt ggaggtggcg
     2581 cacgcggcca acgcgctggt gaaggtgatc atcgaaaccg cgctgctcac cgatgaagaa
     2641 aaggtgattg cctgcctgtt ggccaaggaa gccggggcag attatgtaaa aacctcaacc
     2701 gggttctcgg gcgggggcgc taccgtgcat gatgtcgcgc tgatgcgccg cacggttgga
     2761 ccatcgctgg gggtcaaggc ttcagggggc attcacaccc acgaggaagc tgaagccctg
     2821 gtggcggctg gagccacacg catcggcgcc agcgccgggg tgaagattat ccgggcggac
     2881 caaagcgaga ccaaaccgca gattgccgca cccgagggga aggcctatta ggaggaactc
     2941 atgcttattg ccagagtgat cggcacaacc gtctcaacgg tgaaggatga aaaactgacc
     3001 gggcgcaagc tgctcattgt ccgccagaca gacgagcatg gcgaagcggt tggtaagcca
     3061 tttgtggcgg tggatactgt tgacgctggg gtgggagacc tggtgctcac cgcggcgggc
     3121 tcgagcgccc gccagactgc gatcaccaaa gataccccgg tagatacggt gatcatggcg
     3181 atgatcgaca cgctggaggt cgacggggtg gttactttcc gcaagagctg agggaacaat
     3241 gccggacctt gaccaggacc ttctctcgat tcaagaagcg cgtaacctcg ccacccgggc
     3301 gttcgaagcc tggaagacct gggcgaaagc ttcacaacag gatgtggaca gggtttgtgc
     3361 tgccatggcc gccgctgctt ttaacgccgc tgaccgtctg ggtcaaatgg cacacgaaga
     3421 gaccggttac ggcattccgg cgcataagcg ccttaagaat gaattcgctg ccaggaatgt
     3481 atgggaaagt attaaggata tcaagacagc cggcgtcatt caccatgacc cggaaaaacg
     3541 tatttaccag attgcctggc cgatgggcgt gattgctggt ttgaccccca gtaccaatcc
     3601 gacttctacg gtcatctata aaatactcat cgcggttaaa gcgcgcaatg ccattgtcat
     3661 tgcaccgcac ccctccgcgg cgcggtgcag ttatgaggcc gccgcgttaa tggtaaaagc
     3721 ggctgaagag aacggcgcgc cgccgggttt gatcgcctgt atgcagtacg tcagtctggc
     3781 aggaacgcag gaactcatgg gtcacaagta tacagcgctt atcctggcta ccggcggcac
     3841 gccaatggtg cgcgcggcgc attcaaccgg caaacctgca tatggcgtag gacccggcaa
     3901 tgtgccggct tatgtggacc gctccgctga cctggagaaa gccgcccgct atattaccgc
     3961 cagtaaagct ttcgattgct ccaccatttg tgccactgaa caggctgtgg tggcagaccg
     4021 gccgatcgcg cgttctttgc aacggttgat ggagcaggaa ggcgcgtatt ttgttgataa
     4081 ggcgcagact gacctgctgc gaaggctgct cttcaattca gaaggcgtca tggtccctgc
     4141 tacggtggga aaaccagcgc cttacctggc agcgctggca ggctttaacg taccagcagg
     4201 aacgcgggtg ctggtgacca ggctgaaccg cgtgggcaaa gaagaagcgc tttctcgtga
     4261 aaagctcacc accgtccttg gctggtatga agcggatggc tgggaagccg gctgtgaacg
     4321 ctgcatcgaa ttgatccagt ttggcggcag ggggcacagc cttgtgattc acgccactga
     4381 tgaaaaggtc attatggcgt ttgggctcga aaagccggtt ttccgcatcg cggtgaacac
     4441 catgtcgacg ttgggcgcca ttggnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnngcgtat accgtgctgg tggtactgga cgaagcggga aatccgcttg ccggtgaata
     4621 ccagttcgcc caggtagtgc gcgatggatt ggtggtggat ttttttggcg cggtgaatct
     4681 gctgaaaaca ctgaaagaca aagccgaacg aaggattggc cggctgctca cccgggccgc
     4741 cagcggctac ccgccggggg tgccgcgcgc cgaggtgcgc gcaaccaccc atgtggtgga
     4801 ggctgccggt atggtctgtg agcgcctggt agcggaacct gaggcagcca accaggtttt
     4861 gggaatccac aatggcgtgg tggtggatgt cggcggcggt acaacgggta tcgcggtgct
     4921 ggaaaatggt aaggtggtgt ataccgcgga tgaggctacc ggcggaacac atttttcatt
     4981 ggtcattgcc ggggccaccg gtttgagctt tgaccaggca gaggctctaa aaaagacaac
     5041 ctctgaacag gccggccttt tccccctggt gcggccagta atggaaaaag tgggcaccat
     5101 tattatgcgc catctccgtg gcagcgcgcc tgagtccatc accctggtag gtggcacggc
     5161 gctcttcccg ggcatggctg aggtagttca gcaagttaca ggcattccta cccgggtgcc
     5221 agcgcacccc atgttcatta ccccgctggg aatcgcgctt tcggataacg atactgatcc
     5281 aaggaggcag gatggctgag gatttttcat tacgatgtta ttgtttcatt gaccgcatgc
     5341 aggcacaata cgccgctttt gtgggcacca tcacccaagg agacctgccg gtggagggga
     5401 tggctgcgct ttatgttgag atggcgcctg gcaacgaagt attccgcgtt gtggatatcg
     5461 cggtgaaggc cacggaagcc aaaccaggcg cgcaaattgt ggaacgcgaa tttggcatgt
     5521 tcgaagtgca ttctctcaac caggcggcgg tgattgaagc cgggcaggtg gtgctggaca
     5581 ggctgggatt gaacaaagaa gaccgcatta agcctgaagt ggtttcgacg caaatcatca
     5641 cgcgcattga tccctaccag tcgcagctta tcaaccgctt tcggcgcggc agtatgctgg
     5701 tgccgggaga aacgatgctt gtgctggaat gtgctccggc tgcctacatt aattacgcag
     5761 ttaatgaggc tgaaaaaaag gcgaatatta agatcatcca tgtctcttcg gttggccgat
     5821 ttggccggat gtggctgagc ggcagtgagg cggagatcat taacgcccgg aatgccgcca
     5881 ccagcgcgct cgaaagcctt gaagggatca agggttagcg tagaaaaaag caggagcgag
     5941 tgatgccgaa aaaatttttt acggaacgtg atattgaaga aatggctgcc cgcggcgaaa
     6001 tgttcctgga ggtcagcgac gaagtggtgt taaccgaact ggcgtatgaa aaagctcagt
     6061 gcctgggcgt gcgcctgcac cagacaaatt cgctccctcc cgccgcgccg atacgtcctt
     6121 acctggcgca ggcagtaaat tcctgccctg aatgcagcac atcgcagaaa cccgccgaag
     6181 ctgaactgag gaagcgcatc cgcgatgcgg tggccgcccg gctgggcaca caggtagacc
     6241 cggcgctgct ggagacgatt attacccgtg tgctgaacaa tgtggggatg aagtaaccca
     6301 tgctttttgg tcgcgtccac ggtacggctg tttgcacgat ccgagttccg ggcacggagg
     6361 gcttgaagtt cctggtggtc caacccttaa accgtaagct tgaaccgctg gggcccttac
     6421 aggtggcggt cgacgtggtg caggcgggag tgggagacct gtgtgtgatg gtgcgctcgc
     6481 gtgaagcctc tctggtgatg ccggaggtga agtttgtgcc ggttgacctg gcactggtgg
     6541 gaattgtgga tgagttgagc gtaaaacctg atggcgtatt tgacctggtg atgccgaaag
     6601 gcacttgcga atacacatag gcggattatg attctggcaa aggtcgtcgg aactgtggtt
     6661 accaccatca gccacccaca ctacaagaat cgcaggctgt tggtggttca gccattggtc
     6721 acggagggcg agagcagcga ggaagatttt atcgccattg acaacacgca cgctggaatt
     6781 ggtgacacag tgctcgtcaa ccgtgaagga aacggcgcgc gccaggtgct gaacaaccct
     6841 gatgcctgca tcatctcggt gatcgtgggg attgtggatt cagtgaccat caactgaccc
     6901 caaggtttac aacaacagaa gagcgggtat gcgcgagtcg attggtcgat ggcgctgaca
     6961 tgccaggcga ccagacggct cgagattggc ttgcccgcgg aagctgcgta tacctgccgg
     7021 gtttccaaga ccctgcgatc tcacaaacca acctacgcta cgcgccgggc gaactgaacg
     7081 aggggtattt gccttcgaag cgggctgtat aaaaaacagg attccataag gaatcctgct
     7141 cttgtaaaac gtcattgtgt tattttttgt aattctcagc caggtaaagc gtgagaatgc
     7201 ttctctcttc ttctgtcagg ttggcccctt tggcgctcat ccgggtaacg atggctgccc
     7261 attcttccgc ggtggcggtg gtgtcggtaa ctttcgctag tgtgtggcag gaagcgcagc
     7321 gcgaatcaag cagttccttg ccatcgatca ctggcgcccc aggcggtagt accttaggac
     7381 cgggtgcgtc tgtttgcccc tgcgcggtgg cactggttac atcaactgca acttcggtag
     7441 gagttgaggt ttccacctcg acaggctctt caactgcagc cgtgggcgtg tgaacggctg
     7501 cctttgcgca ggcgttcaaa gaggtgagca ggagaaccat caggatgaaa gagacaacac
     7561 gaaccttcat cgtatcactc taccgaatct tcgatgggtt tacggatggt gacggtaaat
     7621 gccagcagaa taacgccggc agatacgaga atgtaccaga atttcgcata ggcttctacg
     7681 aacgcggggt ctggcaccag ctgcataccc aggaaataaa atttattata ggcgacgacc
     7741 ccagcgagca tcagcagcat ggagatcccc accaacaccc aactgcgcac ggtaaacggc
     7801 ggggtgcctt tgggctgctg cggcagcgga gaaccttttg atctgccttt cacttttcgg
     7861 cgcttgagcg ccaaatccct ttcatagcgc tttttttgac ccctgagcat ggccacgcgc
     7921 aggtaaaaaa tgacaatggc gacgataacg acgatcagtt cagtcgaaat tttactattc
     7981 attttttacg ctccaatcta tttttgatga cccgcttaaa gtcttgctgg gtgggtttca
     8041 acccaatccc gcgttcatgg taattccctt ttggcgttac ctcgccgaaa ctggtgcgat
     8101 tggcatggtc tgtatcatcc ttacgcagca gcacgaacat ggtggagtta taaaacctca
     8161 caatagcgaa ccgccgctca ggtttgggga caagcccgat aaaataaggg tcgccatcct
     8221 ctttgggaga aggcaaggtg atgatgatca ggtggttctt ggagtcgatc acttcctttg
     8281 taaaggtgag cccggccgct gagatgcgtt tctcttccgg taatttatcc cccaggaggt
     8341 cccagtagaa cttcaggaat ttcaccccgt ctttgtcaag gtattttaca aacccgtctg
     8401 tctgactgtg aaacagggcg ggcatagcgt cgaaggcaaa attccattga tggcttttct
     8461 ttttatccat ttcctacttc ctgtgatgat gattttgacc gttctgaccg ttagaaccat
     8521 agcgtaatcg tgactcgtgg gtgcgtttaa caaaatcaac aaattttgtc gcgggtttct
     8581 taagcaggga gttgagcacg tttgggttgc cctgaaaccc gtaatgctcg taatactcga
     8641 acattttcag caaggtgtcg atcgagtttt catcaagacc tgaacgttgc gcgttgaccg
     8701 cccagtctga tcgatcaatg gcgaccgcct tcaccggtct accgatctcc tgagtcagca
     8761 cttccgccat ctcgtcctgg ctgagcgctt gcggtcctgc cagctcatag atggcgtggg
     8821 aatggttcat ctgggtgagc actacttcgg ctgcggcggc tacatcctcc aggtcaacaa
     8881 cgctgatgcg ggcagaggtg tcgtaaggta ccgggtagat cccttcatca atgatagatg
     8941 tccagtttgc caggatgttc tgcatatagg cgcagggttg caggatagtg aaatccaggc
     9001 cagatttgaa gagacactcc tccatgcgca ttttttgcca atgatggggc atctcttcca
     9061 cctgcggatg cagcacagaa tgataaacaa agcgcctgat gcccttttgc ttggccaggg
     9121 taatgagcga acgaccgatt tccacctcgt caggggtcat atttgggcag ataaagtaga
     9181 tcttgtgaat gccatcgagc gcctgtgaaa gcgcctgttg gtcgcgaagg tcagcaatgg
     9241 caacatcggt gctgcccagt tggcgaattt cctgagcttg ttgctcatta cgaaccaggc
     9301 tgcgcacgtg cacaccgtga ggaatgagcc tgtttagaat ggttttaccg gttttcccgg
     9361 ctgcgccagt gagtaagatc aatggtttct cttttcgcca atgaaggatg atttcctgat
     9421 tataatcgat gaaatagaaa aactccccgg tatttttccc cggggagttt tttatcgtgc
     9481 tgttttcgga ggattactcc agaacgtcag ttgagcgcac cttcggcggt ttccaaacca
     9541 ggaagagcag gttgacgatc atgccgaaga cggcgccagc cgcgatcgcg ggccagccga
     9601 aggggaagac acccctgagc agcgggatgg gcaggttgcc gccgtaaccg atgttgccgc
     9661 cgataccgac gacgaggatg agcgcgacaa tcgccagttt acccggatca tacaggtcaa
     9721 ctttttctgc catcatcagg gcgatgccct gcacgccaat gacaccatac aggtagatgg
     9781 cgaggccgcc ggtgaccgca gtcgggatac tgtaaatggc atcgcgcagc gggccgatga
     9841 agccgaggac aacggcgatg gtgccagcgg tgataagcgc cgggccggag tagttgcggg
     9901 tgatcaccat cagcgagttg ttctcaccgt agttggtgcc agcggtggag ccaaagagac
     9961 cgttcaccag gtcgttcagc ccgtcgagca tcaggttgag gccaatatgc ttggcgaaat
    10021 acggtttttc gcgtcccatt tcctccgcca ggtggtcaac atacaggctg atctgataga
    10081 ggtgggcggt ggattctgga atggtggcga tggccatcac acctaccccg atcatcgctg
    10141 ttgccgtaag cgcgttatta aagaccggga acacgatctt gggtacagtc agcagaccgc
    10201 tttgtccaat ggtcgagaaa tcgaccaggc caatgggaat ggcgacaatg tagccgaaga
    10261 tcatggccag gaggatgggg agcatgccga tgaaaccctt accctgcagg tagacggaga
    10321 agatgaccgc ggacaggaag gtgagggcgg ctgctagcca ccacttgagc gttgggggca
    10381 tattgggttc aacaccgcag caggtgccgg aggccatggt cagggcggta aaggcgaggc
    10441 cgataccgat ggtgacggcg accgaaccgg tgatttcagc gggcaaaacc ttatcgaggc
    10501 ctttcttccc gccggttgcg cggatgagca gaccgaccag gatgttgaga ataccagtga
    10561 cgatgaagcc ggcggtagcg acggcgatcg ttgaaggttt ggcgggggcg ccaaaagcca
    10621 ggtcaggttc caagacgccg ataatggagg ccacggctgc gatatagctg aagcttgagc
    10681 cgtagtacag gggaatatag gtattaatcg cgaattttga tccaagtaat gcgacgacag
    10741 tgccaagacc ggtgatggtt aaaacggttg acacatcaaa gtgcatgagc agggcgcaca
    10801 gcacggtggc cgggaacatg gtcagcacgt gctgaaaacc gagcaataac atcctgccaa
    10861 acggcggggt atcctgtggt agataaccca gcactttaga agtagttgcc ataggtttta
    10921 tccttctttc ctttgattaa ttggttttct tcgatcgatt actacttacc tggattgagc
    10981 tcaaaacgaa aaaagataaa tgtcttttcg ctcgccccgt aaacgcaaaa tacgctctcc
    11041 gatacgcacc tccttattaa gttatctatt ccatcttgaa gaaaactaca tatgttaata
    11101 atacatcatt ttgtcaagat ggtgaaaggg ggagattgct ctcccccact gggctggttt
    11161 tcttatttgc ctaaaattat cgccagcaca aggagcacca gcgcaactgg cacaacgtac
    11221 aagatcatgc ggtattcagc gacttccatg aactttttct ttggttccgc tggtttttct
    11281 gcgcttgttg ctgcttcttt cagttcttct gccatctttc ctccaaaatt ttgtcgagtt
    11341 tctcaaacac agttttgaac atggacttcg cgacgctgtc gatcatccgc tgtcccacac
    11401 tggcaagagt accaccaata ttggcctcac ctgtgtattt caacaaggtg gtttcgtcgc
    11461 cgaggtcttc aaacttcaca taaccgaccc ctttgacaaa acccgctccg cctttgccct
    11521 cgatgacgag ggtgacactc tcagggggaa cctcatcggt gaggctgaga ttaccggaga
    11581 agttggcaga aaccggacca acccgaatgc ccagcacggc ttcgtaatgg gattcgtcaa
    11641 gcttgatcat tttttgcatg ccaggaaccg cggctcccag cgcgtcagga tcattgaaca
    11701 tctcgtacac aacttcacgg ggcgctttaa acagatgttc accttcaact ttcataacac
    11761 acttcctcct atttggtttc gcttcactta caggtaatac ttaattgtac acctgaatta
    11821 cttgtgctca acttttttaa gatagccgta gatgctgctt ggactcaggt ttgtttcgag
    11881 aatttcaaca cctttgtcgg cgaacgcgtc ttcaatcgcc tggacgatcg ccggacccgg
    11941 cggaattgca cccgcctctc caacaccctt gatgcccagg gggtttaatt tcagcgaact
    12001 ttcagcatga ttgtggatga tctccagttt cttgggcatg tctgttgcca ggggcatcag
    12061 gtagtccatg aaggatgcgg tcagcaattg accattctcg tcatagacca gcctttcgta
    12121 cagagtgttg ccgataccca tggagatgcc gccctgaagc tgaccatcca cgatcaaggg
    12181 gttgatcatg gtcccgcagt catccacgat aaccatcttc tcaaggactg gtttgcaggt
    12241 ctcttcatcg atgctcagga tcactcccat agcgccgcaa ccagtggagc catgcggagg
    12301 accatagtac gcggtggctt caagccctgg ttcaacgcca ggctcaatga caccgcgcat
    12361 ggggttggcc atggctgcca gggtgcccag gggaattgcg gagtcgggtt tccccttgat
    12421 gctgacgctg ccttcaccca gctcgacctg gtcttcaggt acccctaggg ctttactggc
    12481 ggtggagaag atctttgcgc gtactttctg gctggccagc agggcggccg tgcccaccag
    12541 ggtggcgcca cggctggcga acgtacccgc gccccaggcg aagtggtttg tatcgccggt
    12601 gatgaccttc acatcttcaa ccttcacatt taactggtcc gcgatgattt gagcaaacac
    12661 agtgtagtgc gcctgacctt gagagctgta accggtggtc atggtgacct ttccactggt
    12721 gctcaccgtt accttgcagc cttcgtaagg accaaccgcg gttccttcgg taaaagccac
    12781 aacaccgatg cccagtctct ttccctcttt ttgggctttg ggctgggttt ctttaatgaa
    12841 tttatcgtac tcaatcgcct gcaggcattt ttcaaagttg ccgaggtaat caccgctatc
    12901 cagcgtgttc ttcacaaaat cctgtccggt gataccggtt gccaggggaa gttcgtccgc
    12961 ccgtaaaagg tttttccggc gaatttcgat tggcgaaagg cccatttcct tcgcggcgaa
    13021 atccagcata cgctccatgg cgaacacgcc ttctgaacgt ccggcgccgc gcacgggggt
    13081 gactaccatc tggttggtaa agaccatccg gatttgagtg taaaaattag gcacccggta
    13141 gttgctgata atgtgcgttt gggtgttcag cggcacagtc ataccgtagg agtcataagc
    13201 cccggtattg tggtagaaga catctttgaa ccccaggatg gtgccgtctt tcttgagggc
    13261 gatttcgcag tgatgcacct ggtcgcgttc agaagtggtg gcgaggaagt tctcgcggcg
    13321 atcttcgacc catttaattg ggcgtttcag ttgtttggat atccagggca gcagcacatc
    13381 atcagtcagc gatgtcatca ccttagggcc aaaaccgccg ccaattgccg gggtgatcac
    13441 gtgcacctgg ctctcagcca gacccagggc gcgggataca gagccgcgca cggtcagcgg
    13501 agactgcgta ttcgcccaaa tggtcatcac ctggttctgg tcatcccagc tggcgaggaa
    13561 accgcggttt tccatggcag cgccggcaac gtgttcgatc agcattcgcc tggcaaccac
    13621 cacatcggcc tttttcttcg cctcttcgta attgcccact tcttgggtta ccagggcagc
    13681 catgttggag ggcaggtcat catgaaccag tacagagctg tcatcccagg ctttttccag
    13741 atcgaccacg gcaggcagag attcaacatc cacgatgata tcgtcgaagg cgtcttcggc
    13801 gatatagcgc gattctgcga tcaccaccgc cagtacttca cctgaatagc ggatcttgtc
    13861 tttggcgatg ggcaccagcg tgcgcgcgtt aaacttggaa ccagggatgg cggtaggcgg
    13921 gggcacctgt aaggggccag gcttccagaa atcaccaaaa tcctcggcgg tgtaaacagc
    13981 cacaacccct ggccgcttca atgcttcact cacgtcaatt tttagaattc tggcgtgggc
    14041 gaaatcactg cgcaagaaag ccgcgtgaag ctgaccaggg acttcaatat cgtctatgta
    14101 cttggcgttg cctgtcagca gttgcttatc ttccaagcgc ttgattggat taccgaaata
    14161 ttttccagtc acagtatcac ctcgcttcac tttttctgag acatcttctc ggccgcgtca
    14221 tgcacggctt cgacgatttt ctcatatcct gtgcaccggc agatgttgcc atcaatggcc
    14281 tcgcggatct cgtcttctgt gggtgtgggg ttttcttcga ggaaggggac gagggtcatc
    14341 aagaaaccag gggtgcaaaa accgcactgc agcgcgtgtt tttcggtgaa ggactgctgc
    14401 agcgggtgca ggttttcagg agttccaagc gattccacgg tgcgaatttc gtgaccgtta
    14461 gcctgaaccg cgaaaataag gcaggagcgg acggcttcgc catcgaagag gatggtgcag
    14521 gcaccgcaaa tgccatgttc gcagcccaca tgagttccca gcagacccag gtcatggcgc
    14581 aagaaatcac ttaaaagcag gcgggcttca acctggcgtt catagacttt tccgttaaca
    14641 gttacggtaa tgggaaagag tttcttcatt atttacctcc tcttttcgcg cgttcgaagg
    14701 cttcagtaag gctgcgtacg atcaatacac gggtgatgct gcgacgataa tcagcgctgg
    14761 cgtgcaggtc tgaggccggg tcgatttcgc tggttgaggc ggcttcagcc actgcttcaa
    14821 tcgcatcttt gctgggttta ttgccgatga ggatttcgcg cgctttttct gataggatgg
    14881 ggacctcagc cacactcatc aacaccatgt gcacgtcctt gcagatttcg ttttcatcca
    14941 gggtgacgat agatgcgacc gcagattgcg cgtaaccgcc gctctgtcgg gatacctgga
    15001 cgtacttggc gccggtgcgc ggaggatgag gtttgatgac cagttctgcc agcatctcgt
    15061 tgggctcaat gacggtcatg aacggtccga tgatcaggtc tggcgctgcg acccagcgtt
    15121 cagtaccctt gcccttaatg agcgcctgca tgttcatgac cattgaaatg ctgggcagct
    15181 gccctgcggg gtcagcgtgg gcgattgccc cgccgaaagt accgcggcgg cgaatctgag
    15241 gatgggcgat gaacttggcg acttcgacca gcagcgggaa acgctggatg acatcctggt
    15301 tgtgttccac agtgctgacg cgggtcatag tgccaatcgc caaaccgcca tccgcagttg
    15361 gtttaatgta ggaaagttcc gctacattat tcaggtccac cagcgcgctt ggtctcgcca
    15421 tacggaagtt catcgccgcg atcaaacttt gaccgccggc gaggactttt ccatcgtaac
    15481 caagttccgc cagtgtatcc aacgcttcat caacgctgga gggtgcataa taatcaaaat
    15541 taactggctt cattaattac aacctcctgt taaattgagg attcaaaata ttggggtttc
    15601 aaagaggtat tttgacggta aaaagtgtat cacatgaaaa gttggaacct tcaggggagt
    15661 tactcccctg aaggttcgat cgttaaacgg ctattcctta aaggctacaa agcggcccca
    15721 catagattct gcttccatgg ctttctgcag gtagcaaccg atgtagtagc gcccgctctt
    15781 ggcggtggcg agctgaccag ccaggttctc cgcgtgcaca atggtgtgcg ggaagaggtt
    15841 gaggtgcata tcttggtaga agtctttaag cgggtagagc tcatcccaat ccttgccgaa
    15901 gtccttaatc agcttggcat tggcttcagc aaaggtcttg gggtgccaga tgcgcatgat
    15961 ggtgttcatg gggtggtccg ccgatactgc gtccacgccg atccacttga ttttcatctt
    16021 gatcgcccat tcggagaaat caggtgaagg acccgggtgc ttgaccatgt agcggaactc
    16081 gtcagaatcc ttgctgttcc agccatattt gttccaccca gtcttgatga caaggatgtc
    16141 gccttcacga acttccacct ggtccatgat catctgcggg gtgtaaacac ttgaatcgga
    16201 cacaagcttt gaaatgtcgg cgatcacgcc ggggccgacc caatccttaa ggggaacctg
    16261 gccaatggtg cgaccgttgg ggaagaagtg tttttcacca tccatatgcg tgccgaggtg
    16321 gatgctggcg ttgcagatgg agccgttgcg gcccatgcca aagtattgcc cgccgacgcg
    16381 cttggtatag gtaactgtga gaggcacgta gttgagccac tgcggggttt gcacgctgaa
    16441 gggcacggtc atgtcgatca tttccgattt cttcatcgcg gcaaagaatt cttcctcgct
    16501 catgtgcttg ggggcggtcc aggcgaaaac acggctccag gcggtctcga cttccatgta
    16561 agggatcggc atgatgctga tccaggcgcg tttattcttg agtttgtcga tatcgccgac
    16621 aatagcttct gccagcaaaa ggtgcttttt cggcatgtct atgtgggtca gctggtaata
    16681 aggtccttgt gggaatgtct cgtcccaggt tttgccggtt tctttgagta atttggcttc
    16741 agccagtttg aaatgatctt catgccagcg gcggatgggt gtgttcatgg ggtgctcggc
    16801 tgaaccgcag tccacgccga tgaccttgag ttccatatcc atggcccact tgtaaaagtc
    16861 cattgaagga cccgggtggc ggacgaggaa accgaactcc atgctttcaa caccgccctg
    16921 ggcggtttta ttgcgcaccg ccggctggtc ccaggaatac ttgtggtaac cggtgttgac
    16981 gatgaggatg tcaccctttt tgacctcgac cttggaagtg atcatttcag gggtgtaaat
    17041 actgtaatca gaaaccaggt cagagatatc tgccactacg ccgggcccgc acaggtcggt
    17101 gagcgggata tccgcaatgg cgcgaccgcc ggaattgaac gcccgttcgc ccaccaggtg
    17161 ggtgccaacg gtattgctcc agttgaggat ttgaccattg gcgccctgac cgccaccata
    17221 ggcgccggta acacgcttga agaaatgcac ctcaagccct ttttcatgcg ggtgaggggg
    17281 ggtgaaaatg ctgcagtcct gcgtgaggtc ataaagctcg gcatcggtga gcattttgat
    17341 aaaatcttct cgattcattt ttcctccatt ttttaatatg tttggattag ttatacccgt
    17401 atttgtctgc gaaggcaatt agcgcgtctt cgaaacattt catcggggct gaaacgacgc
    17461 cagcgccaac ctgcccaaca cctgggtctt tatgcgccac accggtgttg actcgcgggg
    17521 tgatttgttt ctcgaccact ttgcggatgt ccacgccagt gggggtgccg cggaaatcaa
    17581 ggaagggaat ggtgaactgc ttgtgttctc cttcagtgat ttcgtacata tccagcgtgg
    17641 tgttgatcgc atcgcgcggt tcaccgccaa caaaggtgac aagggcgggc gcggttgcca
    17701 tggcaaagcc accaatgccg gctgtttcgg tgatcacgct gtcgccgata tccgggcagg
    17761 catctttttc agtgaagcca gggaagtaaa gggctttgac cacaggcgag ggcgcggtga
    17821 accatttgtc tcccaggccg ctcactttga tgccccaatc ggtgccgttg cgcgacatga
    17881 tggtgacgat ggtgctgcct tcaatgttgt gaccggcagc ggtcatggtt ttacatccag
    17941 ccatcaccgg gttgagaatg ctcagcgcgt tctcgcccag gaattttgtg acctcgaagg
    18001 cggtttctgt attcttggct gttttggcga tgtaaggcgc cagttgggtt gtccataaaa
    18061 gtgaagcggt atccaggcgg ttgtgcccat catcaccgct gtgcagcgct ttggcaagga
    18121 tggcgcgcac atcgataccg cccatcgctt cgagcgcggt tttaatggtt gggcccagaa
    18181 cagcctccat ccagcgaagt ttttccagca catcctcgct gtacgcgccc atgcgcagca
    18241 cttttccacg gccttcgttg aggttggagt agcatttaat gccgtgggtc tggttttcga
    18301 tcacatacac cagctgtgaa ggtgaaatga cgcctgccat tggtgcggcg cacatatgct
    18361 catggcaggg ggagaattcg atcttgccgg attttaataa tttttcggct tcttcggggt
    18421 ttttggcttt gccttcaaag agcaaggcgc caatcatggc tcccttggtc gggccggaca
    18481 ttctttcaaa ggtcacgggc gggccggcgt gcaagagcat gttttcgtgc attcccggta
    18541 cgatttccag cgccttcgcc agcgtgatgg cgacggggcg ggcttccatg aatttttcta
    18601 ctgctagtgc gtttgctttt tcgatatcaa ccatttgctt cctccagggt cattatggat
    18661 tatcaggaac tactttctgg gtgatttcat cttttgcaga atattcatca atttttcgtt
    18721 accaccggcg ggcggcttcc aatccacctg cacataaggc gcgccttgat ctttcaggtt
    18781 ggcagcgaag gattcaagac ccacattaat ggcagctaac ggttttttga ttttttcgag
    18841 gtcaaccggt ttcccaagcg cgtgggcttt gggctcatcg cgctcagcca acgcgcgcaa
    18901 gatgcgaccg acgtagcggc agatgacctc gttgctggta tctaccaggg cgcccgcggc
    18961 ttttagctgg gccatctggt tgttcaggtt ctgggggtct tcatctgtgc cgcagaccac
    19021 gcaaaccact tccaggtgcc gtccatcttt cttggcatca gcgatgcatt tggcgatggc
    19081 tggagcgatc tcgctggcgg ggtcgggatg tgagccgtaa ccaatcacca catccaacaa
    19141 aataaccgcc acttcggggt ctttggcttc ttcatacaac cggcgaatgc gcagttcgtt
    19201 atccatcatc gggtgcaggc ggccctgtgt gaattcgtct tcgccgaggt cgacgatgca
    19261 gttctcctgg ctcacgaacg cgtcgggcag ctggaattct ttattaatgg gtgagtttgc
    19321 cagcaccctg gggacgtagc cgtaaaggat gtgctgggct tcgtaagcca gtgtgccgcc
    19381 agagaataaa ccgcgcaggt atttctgttt ggcggtgaac tttttaatcg gcaggtcagc
    19441 atcactgacg agcttgggcg ggttgtgtgc cagctccacc gccagtcggg cggcgtcatc
    19501 caagccggtg gcgaagaaca ggttgccgtc acgcgtggtg gtgattggcc gcgcgatgaa
    19561 gttcaccacg actggtttgc cggctttgcg ggcgaccttc agcacttctt cagccacttt
    19621 gggtgaaggt ggtttggagg taagaacgat caccttggta tcgtcgtctc tcgccagcac
    19681 gtccagtgcc attaaaaagg tgatcgcgcc cacttctgct ttaaggtcgc gaccgccggt
    19741 gccgatgccg aaagtgatac cgctgttcaa ctggtgaata cgggtggcta cctgttgtaa
    19801 accggtgcca gcagcggcaa caatgccaat gggtcctttg cgaatcttgt tggcaaaagc
    19861 cagcccataa ccatcgacca tggcggtgcc gcagtcaggt cccatcacta atagacccag
    19921 gtcgcgggcc tctttcttta attcgatctc attttcaacg ctgacattgt cactgaagag
    19981 gtgcacatgc ttgcgcagcc gcagcgcttc gcgggtgacg gcggtcgcat agcgaccagc
    20041 gactgaaacg atgacccatt ctgcgtcggg cagcatatca gtggcgcttt caatgctccg
    20101 cggctgatat tcctgttctg aaccgccttt acgggcagcc agcaatccat ccacctgggc
    20161 gagcgcgtcc aaagcggctt tttcattctc agctttgatc acaatggcca ggtcgtcagg
    20221 tttggtggcc tttacttcag gggtgtctaa accgatatgg gccagtaatt ctttgtttga
    20281 atctgttccc atcaccacgc cggcatctaa aacgccaggc aacccggcaa gcgaacgttg
    20341 caaagacatg agcttggccg agtcataata aacaccacta cggacttctg ttttaaatgt
    20401 tgccataatc gctccttcat agaatgggta ttagggggtg acacacgcag atgcgtattc
    20461 agaaaaccgc gatcgatttg tgcaccgttc ccttggatga taaagaactt gctttgaacg
    20521 gcgagtggat ggggaaaaac aatctgaccc catggttcac cttcttcaat atatcacgaa
    20581 aaaatcaagc cagtcaactt accttaatcg taaaataaat gacaaataac attatttccc
    20641 aaataaaaga acctcctcat ttgatacggt gcacagagga ggttcttcaa attcataaga
    20701 ctcctaactt acaggtcgac agtctccggt acgcggaagt tttcgttggc tttgcgaact
    20761 tcatcagtta cgttgaagtc catatcctga ccgacgattt cgcggacata gtcttttacg
    20821 cggctatgcc acatatcctg gttaaggtag aagaccttgg cgccgcccag ctcgtgctgg
    20881 tactctggcc agggtttggt aggctgagcc attgaaaggc ctgccatacg ccagccgttg
    20941 tagctttcga aagttgacca ccattccttg accttgagca attccatggc gtaataggtc
    21001 ttggcgtagt acatcggcca gcccaggcga gctttacgag aaaaggtgag tgtgtgcagg
    21061 ctgatggcga tgtctttatc ccaagcgcga ccattggcga aaccgagcca ttcgccatcg
    21121 accaggccga ccatggtgaa aggatctttg aggcgtttgc ggcgcagggc gagaacctca
    21181 ccataaacac gtacacccac aatatcatag aagtccttat caactttcat gaccttttcg
    21241 aggtagccaa gcatgggatc gatgtcgcta tccttgatac cgcggatgta aacgatacga
    21301 ccgtctttta aggtaactgt ggcttccggg atggggggca tatccattgg ggcgccctga
    21361 agaatgcctt cgatctcgcg cagatcgatt ttcatttctt cagttgaaac aggagtcggg
    21421 aattctattt tcattttgca tccttttctt ataatatttt gttgcttaga gtatagcatt
    21481 ccggacaatt gaagtcaaca attgccggat gattaagaag aaaacaggat taatgtaaca
    21541 tcgttcgggt gaaaattatg accttttccg tacggcgaag cagaaatggc tgagtcattc
    21601 aaaaaaccgt gatacagtaa cggtatgaaa acccctctca tactacaaga ccagaaaaaa
    21661 ggagacataa tggataaaga gacacagatg ctcaccatct ttttcaggca tgaccaatcc
    21721 aagagcctgg atgagataca ggctcacctg agggcaaatg gcttctggag ggatttcccg
    21781 ccggagggaa tggaaattgt ctcctgggtg gtgatgatgg gtatcgggca cgtggtcacc
    21841 ctgaggcttc cacaatctcg cttacgcgaa ttaaatatga tcgtcgagaa gaaagcctgg
    21901 aaagccttta cgactgagtt ctaccccacg tatgactttc tcccccatta tcagcagttc
    21961 cacgccgagg cagcagccag ggattaaaaa aagcagccgc aaaggctgct tttttttacg
    22021 agggtaaagt tctattttcc gtactttgcg aaatgcgggg cgaaaatgac ctcggggtca
    22081 ggtatttcag ccgggatagg gtaagaaacc caggtcacat taaggaagtg tttgaccgtg
    22141 atgttctcgc tggtaatgtt gccgccccag gtgccgcaac cgagcgacat gctgaagggc
    22201 atgccgttct cccagtcacc gctgttaccc acgctctgag cctgtctcac catcatgcgg
    22261 ctgactttgg ctttggtcgc cagctccagg atgtgttctt cattgactga atgaataccg
    22321 caagaatggc cgtaaccgct gaagccggtg atatcgatga ccatttgcac tgcctgatcg
    22381 aaggtcttgt acttccacag ggtcagcaca ggtgaaagtt tttcggcaga gaacatatcc
    22441 tcggggccga tgtgttcccc gagcaccatg atgaacttgg tgccatctgg tacgtggatg
    22501 ccggcttctt cagcgatgaa cttgacgctt ttacacacga tcttgccgct tagatgcaca
    22561 ccatctggcc acatggccgc tttcaatttg gcgcggtctt ctcctgttac aaggtgaccg
    22621 ccggttttct ttaattcttc cagcaggtca tcgaagatgc tttcctggat gacgatattg
    22681 ttttcggctg agcaagatgt cgcgttgtca aaaatcttgc ctttgcagat tttcgcggcg
    22741 gtgacaggaa ggtcagcggt ctcatccaca accacagtgg cgttgccagc gcccacgccg
    22801 taggcgggtt ttccgctgga gtaggcgggt ttcactaccc cggcgccccc tgtagccagc
    22861 accaggtcaa ccttgctcat gagttcttgt gtcagttcga tgctgggttc ttcaatgtac
    22921 tgaaccaggt cgagcggcgc gccgacttta gccagacctt tgcgcatttc ttcacaggtc
    22981 accgcgcagg ttttttttgc tctggggtgc ggggcgaaaa tgaccgcgtt tctggctttg
    23041 aggatcatca acccgttaca gggcatggtg gaggaagaat tggtcatggg ggtgatggcg
    23101 ccgataacgc ccatgggttt ggcgattttc accaatcctt tttcgggtac gcgttcgatc
    23161 acgcctaccg ttttttctcc ttgtaaatca cgcagcacgc cgatggtttt cttctggtgt
    23221 ttgaggactt tatcttcata aacccccaat ccggtttcct ctaccgcaag gcgggcgacg
    23281 gtttcagcgg tctcgcgttt gtaggcttcc catcccactg ccagaatcat ctcatctact
    23341 ttttcttgtg gccaaaactc gatcactttt tgcgcttcac gcgctttcgc caacaaatgg
    23401 tcgattaatt cactcattga acctctccta ttgttaatgt atacgtactg ttattatatg
    23461 acagccgcca gtgctgattc aaagatgttt aaggcttcat tcatctggga ttctgtgacc
    23521 accagtggag gtatccagcg aatggtgttg tcccaggggc cggcggtgag cagcatcatc
    23581 tgccggctga ggcattcagc ctgcacggct ttggtggttt ttttatcagg cttgccgtgc
    23641 gcatcacgga attcagtccc caccatcaac cccaacccgc gcacatcagc gatttggggg
    23701 tatttctcct gcaagtgagc caaacccgct tttaactggg cgccgcgggt aacgacgttc
    23761 tcaattattt tctcttcttt tatcgtgcgg atggttgcca ccgcagcggc agccgcgacg
    23821 gcgtttcctc cataggtgcc cccgtgtgat ccagggatcc atttggacat caactcaagc
    23881 cggctaatga cgccagaaat aggcatgcct gaggcgatgc ctttggcaac ggtcatgata
    23941 tccggttcga tttcaaagtg ctgatagccg aaccacttgc ctgtacgacc aaaaccagat
    24001 tgaacttcat cagccaccaa caggatgccg tgtttttggg tcagttcgcg cagacctttc
    24061 ataaaagttt ttggcggaac caggtagccg ccttcaccga taataggctc gatgaagatc
    24121 gcggctgtct cctgcggagc gagctgggtc attaacaaga gctccagttc gtccaggcac
    24181 cactgactgg ctttttcttc gtcccaacca tagcgcagcg cgtatgggaa gggagtgacg
    24241 taaacccccg gcatcagcgg ttgatagcca attttgtaac tattcttgga tgtggtcatg
    24301 gccatggtgg cgttggtacg cccatggaag ctgccctgaa cgacgatgac gttcgggcgc
    24361 ccggtggcat gacgggcaag tttgagcgcg ccttcaaccg cttcagcccc tgagttgctg
    24421 aagaagaagc cgtccagcgt ggagggaaga actgtacgca actcgtctac cagttcaagc
    24481 agcggcttat gcaccaccag gttaacctgc ccatgaatga gcaacccggc ctgctgccgg
    24541 atggcttcta ccactttggg atggcagtga cccgtgttgg tcacgccaat cccgcaggtg
    24601 aaatccaaca tcttgcgccc atccgtggcg tacaggtaac agccttcggc gtggtcaaca
    24661 acgatgtcag taagatgcgt ccaaacgggt gataggtgtt tgaggttttc ttgataattg
    24721 accataagac aatcctttgt gtgagtggaa ttgataagat ggttgagttt gaccacaggc
    24781 gttgagcctg atttctgaag atggaatcat gctcatcccg ctcctgcgat ttgaaacgga
    24841 tgccgggcaa aaaaagaatt ccccaaccaa accacttttt tattatgact caaaagttac
    24901 ggctggggaa ttcatagaaa tcatcactat tctatgaaat tactcaccac tccttcatgc
    24961 cccacgggaa gcccattcca gccatgcgat ccataaaggg cagcgggtca aagaattcag
    25021 gaccttctac accggtggct ttccagatgc cttctgccag cagttccatg ccgatgacgg
    25081 cgttgaaggc tgtttgtgtc accacaacct ggcaatcgaa tttgtccatg ctttctttgt
    25141 tatcggcgac ctggtaaata tacacattcc tttctttacc gtctttcttt cccttgaccc
    25201 aggttccagc gcaggtctgt ccttccatca acgggcccag gtgggcgggg tcgggcaggc
    25261 aggcagccac cacatcgcgc ggggcaacct ctactttccc aaccttgatg ggttctttgt
    25321 tgtccagccc cagcaggtgt aaggtctgca gaatgccaat gaattcatca cctaacccgt
    25381 atttgaaggt tacccgtttg caatcgatat agcgcggtac gagcagcact tcctcgtgct
    25441 ccacgttgac acactcgttg gggccaatgc cttctgggaa gtcgaagacc tctttttcgg
    25501 aaaatggctc ggtagtgaac cagcccttgc ctttttccca gataacaggc ggattcaggc
    25561 attcttcgat ggtcgtccaa atggagaagt tgggggcgaa gtcatagcct ttcacaatga
    25621 ggttggcgcc atcgcgcaca ttcacttcat cgatttcatc aaagaggtgg gtagccgcgt
    25681 acttggcaaa gacgtccgcc atgccgggtt ctacacccaa ccccaacagg gcgaggatgc
    25741 ctttttcttt ccatttatcc gcgcgcgcga actggtaatc tcccagtttg atgcctgtct
    25801 tcgtgtaagg ggtttcaggg tggggatgag agagcgtcat cgccatatcc atatagttgg
    25861 tgcccacatt atacgcggcg tcaaagatgg tttcattaaa gctcgggtcg caggcgttca
    25921 tgatcaggtc tactttgtgc tttttcgcca gctcctcgat catcccgcgg ttgcgcgcgt
    25981 ctacgaattc cacggggaag cgttcattgt ccttgatttt ggcctgtact tccagcgcgc
    26041 gttctttgtt gaagtcagcc agcaccattt tttccaacca gggtcttttg gccgccatta
    26101 tcgcgatggc ttcgccaaca ccacccactc caaccagcaa tactttcata ttatcctcct
    26161 gtgtactagt gattttatgc ttcttctgct gcttctaaca catacggatc cacatcaggg
    26221 atgtctttct ttacccattt gcggattggg aaatacaata ccgcaccaat cgccatggcg
    26281 attaaagcca gcccaacagc cccttgccag ccgtaatcga taatctggct gacgatggca
    26341 atgatgatga tggttaatgg cgccacaaca atgtagatca acccccatat acccccagga
    26401 acccgtttgc gcggaatatc tggttttttg tacctcaaaa cgaccagcgc gaaaatctca
    26461 gccatcagca ccagcatgtt gagaaacaca tcgatgacga ccagattctg gaatgcggag
    26521 gtggagaata tcaggtaaaa tgcgccgcag atgagaatag aaacccacgg cgtgccgtac
    26581 tttggatgaa ctttattcag ccaggttggc atcataccgt cctctgccat cgcgtaaggc
    26641 acccgcgtac ctcctaagcc gttgccaatg aacaagccaa ccatggagaa gatggcagaa
    26701 attcctacaa tcgccccgag gaaataggaa agcgggctgt ctttgcctgc gaccttgtcg
    26761 ccaattcggt gggcaatcgt tggaaactcc cagcccgcat ctgacccggg gtcgataccc
    26821 caggtgagca ttttttcatc cgcgatacca tagtcattaa gaatgggacc aatgcctgtg
    26881 cccggggcgg attcttcgag cccccaggcc agatattgcc cgtcttctcc agctccgcca
    26941 tacaggccgg ctaccgttgg cacggagtaa gaaaggatgg tggcgataag caccagcacc
    27001 atggcacgcg ggtaggtctt cttggggttt ttaatttcat cgcccgcggc agtgggaagc
    27061 tcccaaccca tgaagttcca catggccaca taaataccca cgctcaatgc ccccagcagg
    27121 ttgcgcccac ttacctcagc gccacccggg aggaatggga tgttgctggg ggtgccattg
    27181 acgatccaag cgtaaatgcc aaaaccagac atgataatga tcgggatgat catcgccacg
    27241 cccagccaac tggcggtgag accagagagc ttggcgccgc gaacgttcaa aagagaaatg
    27301 gtgatgatga gcgcggctga taccagccag gagagaaggt cggatgaaaa atgaatcccg
    27361 ccgatggtgg cgccgtcgct gagcgcggga ataaagtagc tcaggtacca ggcggtccac
    27421 accgggtaga tggatacatc cacccacgag accacccagt tcatccatcc tgacataaaa
    27481 ccgatgaatg gtccgaaagc ctgcttgacc cagtgataat aacctccctc tactggcatc
    27541 atggattgca tttcacgcac catcaacatg ttggggatgc taaagacgag cggggtgatg
    27601 accatcaaca gcaaggccaa cccagggcca gagagaccta ccatgggttc tgcgccaaac
    27661 gcgccgccgc aaacggtgaa gtaaatgagc gcgaataacg ggagaatggt cagctccctt
    27721 tttaattttt gcttctctga tctgacgagc gttgccttat cgatctctgg tgccatgtat
    27781 cctcctgtga agatcctgaa gaataaaatc aatcaaaacg cagggagcct gcgttagata
    27841 gtcctggcaa atacataccc ataatggatg ttaaggctca gtcttgcgtc acaataaagg
    27901 aagggattgg ccagacgtgg tcgtctttta tcttaatcgg cacaaaggaa atgtgggttt
    27961 tacgtacccc ggggattttc ccgatgacct cagtgacaaa atcgaacatc gtgctgttat
    28021 caggggtaaa aagctgtatg ctgatatcag tatcaccagt ggcgtaggcg atgtaactta
    28081 ttgactcata ctctgccagt ttcatggcga tagttttgat ttgcgacggt tcgacttcaa
    28141 tgaacacatc cgcgcagacc accagcccaa tattttcagc gttgacattg ccgcgcacgc
    28201 tgatgatgcc gttttctacc aggcggttaa tgcggtagcg taccgcgcgt tcagaaactt
    28261 cgccaccgat gcgtttggcg atttgcgcgc aactcatcct gccgtcttgc agtaagaggt
    28321 cagatatctt cttatcaatc gcgtcgattt ttatcaattt cgacctgaat attgctgaaa
    28381 tatacaataa gtatagataa actttccgaa aatacaagtg tttttgaaaa accg
//
LOCUS       DAOTLW010000026        27934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_2012_length_27934_cov_11.603035, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000026 DAOTLW010000000
VERSION     DAOTLW010000026.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27934
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2012_length_27934_cov_11.603035"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="P5516_05580"
     CDS             <1..445
                     /locus_tag="P5516_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003004369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide ABC transporter ATP-binding protein"
                     /protein_id="HRT91945.1"
                     /translation="TTALDVTIQAQIVDLVKHLREELGMAIIWITHDLGVVASLAHRV
                     MVMYGGYIIEEANVNDLFADPRHPYTVGLLGSLPKIHEDVHKKLFSIEGMPPVLYKKP
                     NACPFAPRCNWVIERCWQENPTLKTVMDGHSVACWVDIRNGSKKR"
     gene            442..1422
                     /locus_tag="P5516_05585"
     CDS             442..1422
                     /locus_tag="P5516_05585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT91946.1"
                     /translation="MTTNNNVLIKVENLVMHFPIYRGVIRRQVGVVHAVDGISFTIQR
                     GETLGLVGESGCGKSTTGRAILQLYKPTAGNVYYKDTNLVKLRPEPMRLMRREMQMIF
                     QDPYASLNPRMTVRDIIGEPLMAFGENNSKQIDERVASLLDMVQLNPDFSSRYPHEFS
                     GGQRQRIGVARALALNPSFIVCDEPISALDVSIQAQVVNLLEELQQELNLTYLFIAHD
                     LSMVRHISNRVAVMYLGTFVELADRTTLYRKPLHPYTQALLSAIPVADPTYNEQRRRI
                     ILEGDVPSPVNPPSGCRFRTRCPLADTICAEQSPEWREVEPGHFAACHKI"
     gene            1532..3556
                     /locus_tag="P5516_05590"
     CDS             1532..3556
                     /locus_tag="P5516_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT91947.1"
                     /translation="MRKVYVLLALLMVVSLILTACAQPTKPPVTEPPAGEEPPVAEPG
                     EVVEVVFNPYLGSNKLDGNGIPPTFFDDVHVRKAMAYCFDYQTVIDDVYMGEAVQSLT
                     LSLPGMPGYSNDTEHYTYDLAKCEEEFKLADVDKDGIPAGEDPDDIWEMGFRFQMLYN
                     TGNTTREIYTEIVNSNLSKVNKKFVVETLGLPWPSYLAAQRAHKVPMMTGGWLEDIHD
                     PHNWYQPYTTGTYGGRQNLPEDLRNQFKALLDKGVGLVKFEDRAEVYKEFNKLYYDQA
                     VGIPLVLSTSHAFEQKWVQGRVMNPIFSSIYYYPISKAAGAKNPDVFTVMTTGSPDTF
                     DPALSYDTASGEIVQNVYETLVFYDGVSTSEYVPQLASEYSVSDDGTVWTFKIREGVK
                     FHEGGDLTASDVAYSFQRGLLQGGYSSPQWMLAEPFLGVGNDDITMIVDEGASADDRE
                     ALMANDPAVLKAACETVMSKIVADDATGTVTMTLAQPWGPFLGTIANGWGSIMDKEWV
                     IEKGGWDGSCDTWQNYYGMVSADDPFTTIANGTGPFKLDYWTQGEEVSLLRNDNYWGT
                     PAALKQVLIKDVPEFGTRFAALKAGDADVIAVPRENATQVDPMVSVAKFYDAETNKYL
                     DDQNICTIDTTILGKGRFEVCAEANDRPLRLYYGLPALSQDVLLFTFLVE"
     gene            3690..4754
                     /locus_tag="P5516_05595"
     CDS             3690..4754
                     /locus_tag="P5516_05595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91948.1"
                     /translation="MTNYIIRRLLLVPVLLFGITLIIFGMLQFLGPVERSALYVKDFP
                     KNDKQIQSVIRHYGLDKPFYQQYWIWLNGVIDPVTGERSGGILHGDFGYSRSQSQPVA
                     NLITNRFPNTLDLALWTVFPMLSVGIWLGVQAAVHQNGVIDQAARIFSIVGTSFPVFV
                     FGLLILMFFYAELGWFPPGKNSNWVTQEIMQGNFHQYTKLLTIDALLNGRLDILWDAL
                     RHMVLPILTLSYITWATFVRVTRSSMLETLRMDYVVTARAKGLKEKDVINKHARPNAM
                     LTIATMAGMSVVGLLGGVVITETVFNYPGIGSAAAEAAQQLDVVTTLGFAIFNGLILI
                     VANLIVDVLYGVIDPRVRLD"
     gene            4765..5805
                     /locus_tag="P5516_05600"
     CDS             4765..5805
                     /locus_tag="P5516_05600"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT91949.1"
                     /translation="MTETPRPTGDNTLLKEVVPDRKISRAERLLGPENYRILKGLLKT
                     PASIIGFILIGIFIFVAVFAPLIAPPVNDDPFKIPRDGFSGQPRPPGTEWKQNVPEVP
                     FWYKPLTGKDEWVHLMGTAQGQYDIFYSVIWGSRTAFRTGLIVVLATVMIGIILGSVS
                     AYYGKWVDQLIMRIVDVFMTVPYIMMALIMAAVLTPVIGRSLVPALLAMITFGWMGYA
                     RIIRSDILSVKQRDFILAARVCGVKDGRILFKHILPNAIFPTLVLASLGIGDVVLSFA
                     ALSFLGIGTPMGYADWGQVLSFARNWITNLADYWYIVVYPGLTLVFFVMGWNLVGDAL
                     RDVLDPKMRGRS"
     gene            5896..7863
                     /locus_tag="P5516_05605"
     CDS             5896..7863
                     /locus_tag="P5516_05605"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT91950.1"
                     /translation="MRMRTRLTSIIALLSVMLAALFLLSACHKEQIAGNPPANTPTVA
                     PPTPTSPPPKTLVVCLANEPGDLYIYGDTSRTKWSVLEALYDGPVDTLNYEPSAVILA
                     TLPSQENGAVSLRSVAVSEGDPVANVNGDIVALKKGTRVFPAGCTSPGCAVEWDGTTA
                     LELTQMVVNFTLLDGVTWSDGEALKAQDSVFSFSVSADEATRVLKTNIERTFSYTALD
                     DHTVEWIGQPGYLTLNPAAFFWSPLPEHALTGLTPAQMNENPLTTRQPLGWGAYKMDE
                     WVSGDHIRLVKNSHYFRAAEGLPYFDVLVYRFIPSTPEVDLSMMLTGECDIIDTSAGL
                     ESQILRVRELEGQGKLKAYFGMGPEWEGLYFGIKPATYDDVYNPYEDRQDFFGDVRVR
                     QAIAYCVDREYIQTYITLNQSVIPSTFLPPNHPFAAAETRSYTHNPQLGIQLLEEAGW
                     QDSDGNHSTPRVAADIKNIFYNTELSLNYYVTESELHAAVTEAVVKSLGECGIKVTPT
                     FLSVGEMFAPGPEGRIFGRNFDLAELAWSTGRQPPCFLFSSSEIPSAKNKWLGTRYGG
                     VNLSGYTSSEYDAACEQALSAGLDRDLLTAQSAHAQQILMEDLPALPLFFHVKAMVSR
                     PDLCGLQLDVSARSPLKNIEALTLGETCPGK"
     gene            7934..8004
                     /locus_tag="P5516_05610"
     tRNA            7934..8004
                     /locus_tag="P5516_05610"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:7966..7968,aa:Gln,seq:ttg)
     gene            8156..9499
                     /gene="glmU"
                     /locus_tag="P5516_05615"
     CDS             8156..9499
                     /gene="glmU"
                     /locus_tag="P5516_05615"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HRT91951.1"
                     /translation="MNLSTIILAAGQGTRMNSTLPKVLHPLNGKLLVKYSIDLADALS
                     SSRTVVVIGNGAEQVRSALGESVAYALQPQRLGTANAVQAAEGLLADASGLVLVISAD
                     MPLLTADTLQRLVTTQSQNTGPMALLTVRSANPRGFGRILRGVDGGVTGIVEEAQATA
                     EQLKIDELNVGAYCFNSSWLWQALRKIQLSPKGEYYLTDIVAVAVAEGSPVSSLTVTD
                     EEETLGINNRVHLAEAERVIRRRINQAHMLAGVSMTDPELVYIEESVKIGRDTILLPN
                     TWLSGSTIIGEGCEIGPNTFINDSTIGNHCHLLASVVEGAVVEDEVGMGPFCHLRKGA
                     HLGRGVHMGNFGEVKDSYLAAGVKMGHFSYIGNATIGENVNIGAGTITCNFDGVNKNP
                     TEIGENTFIGSDTMLVAPVVIGKNAKTAAGAVVTHDVADNEVVAGVPARPMKKKE"
     gene            9501..10763
                     /locus_tag="P5516_05620"
     CDS             9501..10763
                     /locus_tag="P5516_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013741.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HRT91952.1"
                     /translation="MTLIAWLLLGVFVALDIFICIIRAALFNARLPQLIDLGTEDREE
                     LERTIKVLETTRLRATMRLLVAVLHALVLASLWLLLKTTYTQLSIGGLLGIFALAIFT
                     LLCFEFLAERFPLKDPETWAMRLAPLANFLDRLFSPFTSLLVGLQGSGDTVQRNLGAV
                     SEDELKTWVEVGEPEGGLEPDERKMIYSIFQFGDTLCREIMVPRIEVLALDVDTPLND
                     AINQFITTGHSRAPVYEEVIDNVIGLLYAKDLLKLLTSPAKNQTIRKFLRPAYFVPES
                     KKADELLAEMQARGIHLAVVVDEYGGMAGLVTLEDIVEEIVGEIRDEYDQSEERLVEK
                     ISDNEFVFLGRVSLDDFNEALDTELESDQTDTLGGYFYSQEGRVPVEGDELVFDGWRL
                     TVEGVHHRRVGRIRAVKMEKQDEDNDTN"
     gene            10741..11157
                     /gene="cdd"
                     /locus_tag="P5516_05625"
     CDS             10741..11157
                     /gene="cdd"
                     /locus_tag="P5516_05625"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HRT91953.1"
                     /translation="MKTTTQIDGEMLEKLIETARNARNWAYAPYSHYAVGAALLTASG
                     KIYDGVNIENAAFPAGICAERVAMFKAVSEGERDFTFLVVMTDNGGSPCGSCRQVMAE
                     FGLSTRVLIVDTNGKTHIDTTVEGLLPWAFRPEDLL"
     gene            11164..11928
                     /gene="recO"
                     /locus_tag="P5516_05630"
     CDS             11164..11928
                     /gene="recO"
                     /locus_tag="P5516_05630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HRT91954.1"
                     /translation="MPRSERNIRVEAVVLRHSDWGEADRLLVIYSREQGKLRAIAKGL
                     RKIQSRKAGHLEPFTRAAIMLAKGHDLWIVTQAEAIDLFQPLRENLQKMGQAAYVVEL
                     LDRFTYEEGQNWQLYQLLTETLTRLSTTEEDFVPVKYYEMRLLDLLGYRPLLFECAAC
                     AEAITARDQYFSADMGGVLCPKCATRGQSSRPVSMEALRFLRHFQRSNYKEAMRAAPP
                     PAIRQEIDALQHYYLTYLLERSLNSPEFLKQIRKQM"
     gene            12008..15004
                     /gene="glyS"
                     /locus_tag="P5516_05635"
     CDS             12008..15004
                     /gene="glyS"
                     /locus_tag="P5516_05635"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HRT91955.1"
                     /translation="MSKAFAFQSVIMSLQKFWADQGCLIWQPYYSQVGAGTMNPATFL
                     RVLGPEPWKVAYVEPSIRPDDGRYGENPNRFQQHYQFQVILKPDPGNPQEIYLESLKA
                     IGIDPREHDIRFVEDNWQQPALGAWGLGWEVWLDGQEITQFTYFQQAGGLMLDPVSVE
                     ITYGLERITMPLQRVHHFREMRWNDTLTDGDVNLQAEVEHSKYYFEVADVERMRQLYQ
                     LYQAEAAEALGKGLVLPAYDYLLKCSHTFNILDTRGAMGVTERQAMFGKMREMARAIS
                     EAYVAQRQELGFPWMRGEKKTSAVEVKPIETHVFPDAPTDLLLEIGTEELPVADLQSA
                     IEQLIERVPLMLDELRLTHGQVSVAGTPRRLVVIVEDLAARQEAKETLVKGPPASRGY
                     DANGLPTKAAEGFAQSKGVRVQDLRAREIDGGTYLVALVKEDTRPTHQVLGEALPGLL
                     ESIKFEKTMRWNHTNVPFSRPVRWLTALLGGSTIPFEFAGLRATNTTHGLRFNEPTEI
                     TLTSLADYQAFLSSQGIILDPLRRKKTIQQQVNERCEQVGGRPLLEEELLEEVSRLVE
                     APTALLGRFDPAHLELPPEVLISVMKKHQRYFPVCNDAGKLLPFFVVVRNGDGHGADV
                     VTDGNEQVIKARFADAQFFIKEDMKHKLEDMLVRLGSLTFQHKLGSMLDKSRRVTLLA
                     DPLSGALGLSAAEKLTALRAAELCKADLVTHMVVEMTSLQGIMGQYYALHSGETQAVA
                     DAIREHYLPRYTGDSLPGSKAGLVVGLADRLDSLVGLFGAGLAPTGTRDPFAQRRTAI
                     GLVQLLTAFDIEFDLRQWLELAAQNLPLTISVENMQTCADFITARLRNILIEQGARYD
                     VVDAVLAEQANNPASAQRGVKELGNWVEREDWNSILPAYARCVRITRDQKQIYPVNPA
                     AFADPTEEALHEALVKAEQTPRRAGSVRDSLNVFLPMIPAVNAFFDSVLVMAEGPGLR
                     TNRLGLLQRVSAIMRGVADLSKLEGF"
     gene            15053..16948
                     /gene="dnaG"
                     /locus_tag="P5516_05640"
     CDS             15053..16948
                     /gene="dnaG"
                     /locus_tag="P5516_05640"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HRT91956.1"
                     /translation="MTAIDEIKARLDIVDIVSETVKLRRSGRSYSGFCPFHPNSKTPA
                     FAVFPDNGTWHCFGACNEGGDIFKFVMKREGWDFAETLKYLAERAGVTLTPMTPERKE
                     KEDQYEVMRQLLEESVKYYQFQLTKTPAGRAAYDYLIQRGLTPASIEAFELGYAPQSY
                     EALLGHLSGKGHALEQMLEVGLLVEKDSGGYYDKFRNRIMFPIRDGAGKMAGFGARVL
                     NPEDVPKFLNSPQTPLFNKGRLLYGLNKAKRAIRDADQVVLVEGYLDVIALHQNGFTN
                     SVSPMGTALNEDQLRYLKKYTRRIVLALDADAAGEKATLRGVEIARQALDREAELTYD
                     PRGLLKHEARLQADVRVTTLPPGLDPDDVVLKDRQAWQQVLESAKPIVVHVMETLAAG
                     QNLEDAKVKSAIAAQVTPLIEDVPSAVERDTYRQQLARLLKVDERSLMGLQKVRGRTR
                     RASAEAAQGTQMARSTPGARTVDSRTLQMEKHILSLLLNDTDRYYELERRIKSAGVEP
                     LSVADFDDAQNHELIKLLMKAMRQDAQEALDYLRENTTGDFTERLDELLSETNIKNTQ
                     TETILSDLYRTLISLRLIRVNQVINQLRFLQEEESADRDDQALQSMILSHTIARGKLD
                     LALAKTQ"
     gene            17244..17408
                     /locus_tag="P5516_05645"
                     /pseudo
     CDS             17244..17408
                     /locus_tag="P5516_05645"
                     /inference="COORDINATES: protein motif:HMM:NF012367.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 factor domain-containing protein"
     gene            17880..18689
                     /locus_tag="P5516_05650"
                     /pseudo
     CDS             17880..18689
                     /locus_tag="P5516_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
     gene            complement(18708..19550)
                     /locus_tag="P5516_05655"
     CDS             complement(18708..19550)
                     /locus_tag="P5516_05655"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRT91957.1"
                     /translation="MRSFFNVGLLVGLCFAILLSSCAPRLAAAPLIVDPPSTPIPTLT
                     STPPATATVEIPAPVEPTFTPTSAACQINRVKAIPVFDQNGLLKSFDTPDKFIVLTID
                     DGYNNEVLNQMLDILEEHGARATFFLVGTSFGKNIQRDTFQRLVDNGHDIGYHSLTHP
                     DVNIIDGMSQEEWLQDYQDWSDALRAVIGDDLYSRGVTPYVRAPWGRWTAEFMQAAHK
                     QGFTPVYWSADEHTFEPNRMPLKPGGILILHIIPENLDELQQLMKTDWQIISLREALS
                     ETCN"
     gene            19796..21067
                     /gene="rho"
                     /locus_tag="P5516_05660"
     CDS             19796..21067
                     /gene="rho"
                     /locus_tag="P5516_05660"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HRT91958.1"
                     /translation="MKIIEMEKASLSSLRAIAEELKVPNAKRLKKENLIINIRRAEAE
                     QEGLEVRGGVLEITPEGVGFLRTNYSIGAEDVYVSQAQLRRHELRMGDLVIGVVRPPR
                     DAERHYGLLKVESVNGLAPDEMRNRKTFESLTPIFPDKRFDLETDKSILSSRLINLIA
                     PIGRGQRGMIVSPPKAGKTTILKQLANSITANNPEIHLIIALIGERPEEVTDMDRSVD
                     AEVVASTFDEPVSAHVRTAEITLERAKRLVEIGRDVVILMDSLTRLARAYNLVVNPSG
                     RTLSGGMDPSALYPPKKFFGAARNIEEGGSLTIIATALVDTGSRLDDVVYEEFKGTGN
                     MELHLSRRLQERRVFPAIDIERSSTRREDLLLGPDILPRVWLMRRMYLQMIATPPQGA
                     GMDPAVATEAILQRMARTKDNMEFLENLTED"
     gene            21068..22180
                     /locus_tag="P5516_05665"
     CDS             21068..22180
                     /locus_tag="P5516_05665"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRT91959.1"
                     /translation="MTTKKTAPKVAVEPEEQTTPVKPAQPAPSWTVILSWVGTLFLVA
                     ILLAVLFRFNPADREKKSHEPLNVASDAQVELPALTSAQADYSLVRAASLDTTIPEGV
                     RHFAIKHTVEEGDSIFAIAKKYNLKPESILWANYDLLHDDPTYLNPGWQLTIPPTDGI
                     YYKLKEGDTLEKLAEKYYANVDDIISWPSNHMDITTPSVAGLEYVMIPGGYREIQSWI
                     VPLAYTSGSGATREIAGPGGCGSTNYVLIGSTAFIWPAVQYDLSGFDFSSYHLAIDIA
                     AGVGDAVYASDSGTVVYAGWNDSGYGNLVVIDHGNGYETLYAHLSQVYVSCGQGVYRG
                     GVIGAAGNTGRSFGSHLHFEVRLNGGFVNPWSVLGY"
     gene            complement(22232..24727)
                     /gene="priA"
                     /locus_tag="P5516_05670"
     CDS             complement(22232..24727)
                     /gene="priA"
                     /locus_tag="P5516_05670"
                     /inference="COORDINATES: protein motif:HMM:TIGR00595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005658 - alpha DNA polymerase:primase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HRT91960.1"
                     /translation="MSNYARVSVNIAPLTGLFDYQVPDEYAPHIQPGSLVTVPFGRQT
                     AQGVVISLLETPAVDQTRPIATVVDDTPVLTSWQMRLAEQMARDNLATLPQCLDLMVP
                     PGLSQHADTLVTRSKQARTTALSSVQQRLLDLLEKRGPLRGRQVDTALPRVNWRESLS
                     ALVKAQLVVTQSVLPSPSVRPKVVRTALFSAPRGWRDDPGILVGRASSQAAQRRVAAL
                     EFLERQAIPVNISWVYAESGASSADLNRLAEMGLIILGETEIWRDPLEKLQPVLIHPP
                     VLTNHQQKVWEEIHHQLVNPSTPVPNLLLGVTGSGKTELYLRAAAAVVEKGRGVFILV
                     PEISLTPQTVKRFFARFPGKVGLIHSKLSPGERYDTWRRIRAGLLPIVIGPRSALFSP
                     LSDPGLIVIDEAHDPSYRQEDTQPHYHTVQCALSLSQINGACLLLGSATPDVETLYRF
                     TENHWPIHHLPQRILAHTSPDGQQPPSTQPNFTDLPQVEVVDMRKELSAGNRSAISQS
                     LHTALEEVLQRNEQAILFLNRRGSASYVFCRDCGFVLRCPRCETQLTWHADENQLICH
                     TCNYRRALPSQCPSCGGMNIRQFGMGTESLEKYLQGQFPSARLLRWDADTARYRGAHD
                     LILDHFIQHRADVLIGTQMLAKGLDLPLVTLVGVVLADISLNLPDFRAAERTYQLLTQ
                     VAGRAGRSARGGKVIFQTFQPGHYAIQAAAAYDAEGFYRTELAYRERTGYPPFSRLLK
                     IEFRHTDAAKAQHSAQTAGGQLKHWIDTENYRNTSLIGPVPCFYQKRAGYYRWQVIIR
                     GPDPRVVLEQHTFPDWKDPGLLVDLNLDPLNLL"
     gene            complement(24736..25578)
                     /gene="amrB"
                     /locus_tag="P5516_05675"
     CDS             complement(24736..25578)
                     /gene="amrB"
                     /locus_tag="P5516_05675"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HRT91961.1"
                     /translation="MNPHPPIRRSPIAGAWYSDNPHILRNEIKAYIDAATPPALPGEV
                     TGLIAPHAGYFYSGATAGHAYRCVKGRAFDVCVVFSPLHDYLPSQLLTSSHLAYATPL
                     GEVPINQHLVEQCSANIAKATGEILQPIANDREHSLEIQLPFLQVALSAPFSLVPIMM
                     RSQDPALLTAAAAAIAPALKEQNALLVASTDLSHFYTQSEAIQLDTSMLQQISAFSPE
                     GVLRAERCGEGFACGAGAVALMLWTEKELGATRVTLLHHSTSADSSGDASRVVGYGAL
                     AVTR"
     gene            complement(25664..27181)
                     /gene="lysS"
                     /locus_tag="P5516_05680"
     CDS             complement(25664..27181)
                     /gene="lysS"
                     /locus_tag="P5516_05680"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HRT91962.1"
                     /translation="MELNELERVRCEKIQQMRDSGFEPYPTRTEVTSTTADAIKAFEN
                     AEKAGKPETLTTVLGGRLRAVRLMGKLAFAHIEDGSGRIQLFFRVNELGEAEMQRFKD
                     HFDLGDFIQAGGDLVRTRTGEVSLLVRNFKMLAKAITPLPAAKDEVVDGQVVRHATLS
                     DPETRYRQRYADLAVNQEVRQIFRTRAAVVRALRQFLDSRGFLEVETPILQPIYGGAA
                     AKPFVTHHNQLKQDLYLRISFELYLKRLTVGGFDRVYEIGRDFRNEGVSYKHNPEFTQ
                     LEFYWAYADYLKVMEMTEEMIAYVCDSVFGTRKLTYDGVELDFNPPWQRVNLRQSLIE
                     KANIDISLYPTAEALEEMMVSLDMKVKPGEARGKLIEQLLEEFVEPTLVQPTFMIDYP
                     RDISPLAKNLPGDPSTVERFEGFVCGMELCNAFTELNDPLEQEKRFLEMGRDYKADDE
                     EQHPMDEDYLKAMSYGMPPQGGFGMGIDRLVMLLTDQKNIREVLLFPPLRERDDQ"
     gene            complement(27290..27760)
                     /gene="greA"
                     /locus_tag="P5516_05685"
     CDS             complement(27290..27760)
                     /gene="greA"
                     /locus_tag="P5516_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HRT91963.1"
                     /translation="MPTPVLTTEGYQKLQDELEYLRTTRREQIAQRLHEAIEGGELLD
                     NAELEAAKNEQAFVEGSIKELEILLATARVVNEAEIDKETVQVGNRVTIQEIGATQKE
                     EYVIVGAAEADPTLGKISNESPMGKALLGKRVGDKVQIEAPAGAYTVKVLKVDL"
BASE COUNT         6453 a         7309 c         7802 g         6370 t
ORIGIN      
        1 aaccaccgcg ctggatgtga ccatccaggc gcaaatcgtt gacctggtca aacacttgcg
       61 cgaagaactg ggaatggcta tcatctggat cacccatgac cttggggtgg tggcgagcct
      121 ggctcaccgg gtgatggtga tgtacggcgg atacataatc gaagaagcca atgtgaatga
      181 tctttttgct gacccgagac acccgtatac ggtgggtctg ctgggcagcc tgccgaaaat
      241 ccacgaagat gtgcacaaaa aactattttc aattgagggg atgccccctg ttttgtacaa
      301 gaaaccaaac gcatgcccgt tcgcaccacg ctgtaactgg gtgatcgaac gctgctggca
      361 agaaaacccc accctgaaaa cagtaatgga tggacactct gtagcctgct gggtcgacat
      421 caggaacggg agtaagaaac gatgaccacc aacaacaacg tattgattaa ggttgaaaac
      481 ctggtaatgc acttcccaat ctatcgtggt gtcatccgcc gccaggtagg tgtggttcac
      541 gctgtagatg gcatttcttt cacaattcaa cgcggagaaa cgcttggcct ggtcggcgaa
      601 tcaggctgcg gtaagtccac cacggggcgc gcgatcttac aactttataa gccaaccgct
      661 ggcaatgtct actataaaga taccaacctg gttaagttac gccctgaacc catgcgcttg
      721 atgcgccgag aaatgcagat gatctttcaa gacccttacg ccagccttaa cccgcgcatg
      781 accgtgcgcg acattatcgg tgaaccattg atggcgtttg gagaaaataa cagcaagcaa
      841 attgatgaaa gagttgcttc tctgctggac atggtacaac tcaaccccga tttttccagc
      901 cgttatcctc acgagttttc aggtggtcag cgccagcgca ttggggtggc ccgggcactg
      961 gccttaaacc cttctttcat cgtctgcgac gaacccattt cggcgctgga tgtttccatc
     1021 caggcgcagg tggtgaacct gttggaagaa ctccagcaag aattgaacct gacctatctc
     1081 ttcatcgcgc acgatctttc aatggtgcgc cacatcagca accgtgtagc ggtgatgtac
     1141 ctgggtacgt ttgtggagct ggcggatcgc accactttat atcgcaaacc cctccaccct
     1201 tatacccagg ccttactttc tgcgataccg gtggccgacc ctacctacaa cgagcaacgc
     1261 cgccgtatca tactggaagg cgatgtacct tcccctgtca accccccgtc aggatgccgc
     1321 ttccgcaccc gctgccccct tgcggacacg atttgcgcgg agcagtcacc tgaatggcgg
     1381 gaagtcgagc cgggacattt cgcggcgtgt cataaaattt aaccgaaggg taaacacgag
     1441 gaggtgatga caattctggt gaagatgctg cccataccgt atttgttgtc tgtaaccgaa
     1501 ttttcaaaag ttctcacaag gaggagaaaa aatgcgtaag gtatatgtac tgcttgcttt
     1561 gttgatggtc gtaagcttga ttcttacagc ctgcgcccag ccaaccaagc cccctgtgac
     1621 cgagccccct gcgggtgaag aaccccctgt ggcagaacct ggtgaggtcg tcgaggttgt
     1681 ttttaaccct tacctgggtt ccaataagtt ggatggaaat ggcattccgc ccacattctt
     1741 cgatgatgtc catgtccgca aggcgatggc ttactgcttc gattatcaga cagtcatcga
     1801 cgatgtctac atgggcgaag ccgttcaatc cttaaccctc tctctgcctg gcatgccggg
     1861 gtattcaaac gataccgagc attacacgta tgacctggca aaatgcgagg aagagttcaa
     1921 actggctgat gtggacaaag acggcatccc ggctggtgaa gatcccgatg atatctggga
     1981 aatgggtttt cgcttccaga tgctttacaa caccggtaac accacccgcg agatttacac
     2041 cgagatcgtg aactcaaacc tcagcaaagt gaacaaaaag ttcgtggttg agaccctcgg
     2101 tttaccgtgg ccttcctatc tggctgccca acgcgcccac aaggtgccca tgatgactgg
     2161 cggctggctt gaagatatcc acgacccgca caactggtac cagccttaca ccaccggcac
     2221 ttatggcggt cggcagaatt tacctgaaga cctgaggaat cagttcaaag cgctgttgga
     2281 taagggtgtg ggcctggtga aattcgagga tcgcgccgag gtttacaaag aatttaacaa
     2341 gctgtattat gatcaggcag ttggtattcc cctggtactc tccacttccc acgctttcga
     2401 gcagaagtgg gtgcagggtc gcgtgatgaa cccgatcttc tccagcattt actactaccc
     2461 gatctcaaaa gcggcaggcg ccaagaaccc cgatgtcttc actgtgatga ccaccggttc
     2521 acccgatacc ttcgacccgg cgctgtctta tgacaccgcc agcggagaga tcgtccagaa
     2581 cgtgtacgaa accctggtct tctatgatgg cgtttccacc agcgaatatg ttccccagct
     2641 ggcatctgaa tactcagtat cagatgatgg aactgtctgg accttcaaaa tccgtgaagg
     2701 cgtcaaattc cacgagggcg gcgacctgac cgcttctgat gtggcatatt ccttccagcg
     2761 tggacttcta cagggaggat attcctcacc gcagtggatg ttggcggagc ctttcctcgg
     2821 tgtcggcaac gacgatatca ccatgatcgt cgatgaaggc gcttctgctg atgatcgcga
     2881 agcactgatg gccaatgatc cggctgttct gaaagccgcc tgcgagactg taatgtctaa
     2941 aatcgtggct gatgatgcca ccggtacggt aacgatgacg cttgcacagc cctggggtcc
     3001 cttccttggc accatcgcca atggctgggg ttccattatg gataaagaat gggtcatcga
     3061 aaaaggtggt tgggatggct cctgcgatac ctggcagaac tactatggta tggtgtctgc
     3121 tgacgatccg tttaccacga tcgccaatgg taccggcccg ttcaaattgg attactggac
     3181 acagggcgaa gaagtatccc tgctccgcaa tgacaattac tggggtaccc ccgctgcgtt
     3241 aaagcaggtg cttatcaaag acgttcccga attcggtacc cgcttcgccg cgcttaaggc
     3301 tggtgacgct gatgtcatcg ctgtgccgag agaaaatgca acccaggtgg atccaatggt
     3361 gagcgttgcc aagttctacg acgcagagac caacaaatac cttgatgatc agaacatctg
     3421 caccatcgat accactatcc tgggcaaagg acgctttgag gtttgcgctg aagccaatga
     3481 ccgcccgctg cgcttgtact acggtctgcc ggcgctgtca caggatgtac tgctgttcac
     3541 gttcttagtc gagtaagcga gaaccgctct gtaagttatt tgcgggagcc tttaaaaggc
     3601 tcccgcaaaa accttgattt ttctttgcgc aatgcattga attgcattca atccattccg
     3661 cacagaaaaa cctgatttga ggtgtttcga tgaccaacta cataatccgc cgtttattac
     3721 tggtcccggt gctgctattt ggcattacac tgatcatctt tggtatgctt cagttcctgg
     3781 gcccggtgga gcggtcagcc ttatatgtaa aagacttccc caagaatgat aaacagattc
     3841 aaagtgttat ccggcattat ggtttagaca aacccttcta ccaacaatat tggatctggt
     3901 tgaacggcgt gattgacccg gttacaggag aacgtagtgg tggtattttg cacggtgatt
     3961 ttggctattc acgttctcaa tctcaaccgg tggctaacct tattaccaac aggtttccta
     4021 acacgcttga cctggcgctg tggacggtct tccctatgct ttcagtaggg atttggttgg
     4081 gggtgcaggc agccgttcac cagaatggcg tcatcgacca ggccgccagg atattcagta
     4141 ttgtgggcac ttctttcccg gtctttgtgt ttgggctgct gatcttgatg tttttctacg
     4201 cggaactggg ttggtttccg ccggggaaga attcaaactg ggtgacgcag gaaattatgc
     4261 aggggaattt tcaccaatac accaaattgc tcaccatcga cgcgctgctg aatggacggc
     4321 tggatattct ttgggacgcg ctgcgccata tggtgctgcc aatcctgacc ctttcgtaca
     4381 tcacctgggc aacttttgtg cgcgttaccc gttcttcgat gcttgaaacc ctgcgtatgg
     4441 attacgtggt caccgcacgc gctaaagggc ttaaagaaaa agatgtgatc aataaacacg
     4501 ctcgcccaaa cgccatgctt accattgcta cgatggctgg tatgagcgtg gtgggtttgc
     4561 tgggcggcgt ggtgattaca gaaaccgtat ttaattaccc cggcatcggg tccgcggcag
     4621 ccgaagcggc tcaacaactg gatgtggtca ccacacttgg gttcgcgatc tttaacggtc
     4681 tgatcttaat tgtggctaac ctgatcgtcg atgtattgta cggcgtcata gacccgcgcg
     4741 tgcgcctgga ctgaggagaa caacatgact gaaacaccaa gaccgacagg tgacaacacc
     4801 ctgctaaaag aagtggtacc agaccgcaaa atcagccggg cggaaagact ccttgggcct
     4861 gaaaactatc gcatccttaa gggtttgtta aaaacccccg cgtcgattat cggcttcatc
     4921 ctgattggaa tatttatttt cgtcgcggtt tttgcgccgc tgatcgctcc accggtgaat
     4981 gatgaccctt tcaaaatccc gcgagatggc ttcagcggtc aaccccgccc accgggcact
     5041 gagtggaagc aaaatgtacc agaggtgcct ttttggtaca aaccactgac cggtaaggat
     5101 gaatgggtgc acctgatggg aaccgcccag gggcagtatg atatattcta cagcgtcatc
     5161 tggggttccc ggactgcttt ccgcactgga ttgatcgttg tattggctac agtgatgatt
     5221 ggtatcatcc ttggttcagt ctctgcgtat tacggcaaat gggttgacca gctgatcatg
     5281 cgcattgtgg atgtttttat gactgtaccc tatattatga tggccttgat catggcggcg
     5341 gtactgaccc cggtcattgg caggagcctg gttcctgcct tactggcgat gatcaccttt
     5401 ggctggatgg gttacgcgcg tattattcgc agcgatatcc tctcggtcaa acagcgcgat
     5461 tttattctgg cggcgcgtgt gtgcggggtg aaagacggtc gtatcttgtt taagcatatc
     5521 ctgccaaacg ccatcttccc caccctggta ctggcttcgc ttggtattgg cgatgtggtg
     5581 ctttccttcg cggcgctgtc ctttctcggt atcggcactc ccatgggtta cgcggattgg
     5641 gggcaggtgc tcagttttgc gcgcaactgg atcaccaacc tggctgacta ctggtatatc
     5701 gtcgtttacc ctggtttgac gctggtgttt ttcgtgatgg gctggaacct ggtgggggat
     5761 gccctgcgcg atgtgttaga ccccaaaatg cgcggacggt cataaagcgc gcactcgaac
     5821 ctcaacggcg gcagctcaca gccgccgttt tttgttcccg gtctatgctt tataattgac
     5881 acgaggataa tcgatatgag aatgagaaca aggcttacat ctattatagc cctgctgtcg
     5941 gtgatgctgg cagcgctatt tttactgagc gcctgccaca aagaacaaat tgctggcaac
     6001 ccgcctgcca atacgcccac cgtggcaccg cccactccca cgtcgccgcc ccccaagacg
     6061 ctggtcgtgt gcctggccaa tgaacccggt gacctgtaca tctatggcga tacttcgcgt
     6121 accaagtgga gcgtgctaga ggcgctttat gatggaccgg tcgacacgct caattatgaa
     6181 ccatccgcgg taatcctggc tactctccct tcgcaggaaa atggcgcggt gagcctgcgc
     6241 tctgtggcgg tcagtgaggg tgacccggtc gcaaatgtga acggagacat cgtcgcgctt
     6301 aagaagggca cgcgcgtttt cccggcgggc tgcacctctc ctggctgcgc ggtagagtgg
     6361 gacggcacga ccgcgctcga attgactcag atggtggtga acttcaccct gctggacggc
     6421 gtcacctggt cagatggcga agcgcttaaa gcgcaagatt cggttttctc cttcagcgtc
     6481 agcgccgacg aggcaaccag ggtccttaaa accaacatcg agcgtacttt ctcatatact
     6541 gcgctggatg atcacacggt ggaatggatc ggtcagccag gctatcttac gcttaacccc
     6601 gcggcttttt tctggtcgcc gctgccagaa cacgcgctca ccgggctcac tcctgcccag
     6661 atgaacgaaa accccctgac cacgcgccag ccccttggtt ggggcgcgta taagatggac
     6721 gaatgggtga gcggcgacca catccgcctg gtgaagaact ctcattactt cagagcggct
     6781 gaaggtttgc cctatttcga tgtgcttgtg taccgcttca ttccctccac gcctgaggtt
     6841 gacctgagca tgatgttgac cggtgaatgc gacatcatag atacctccgc cgggttggag
     6901 tcacagatcc tgcgcgtgcg tgaactggaa ggacagggca agctcaaagc ttactttggc
     6961 atgggtccag aatgggaggg gctgtacttc ggtattaaac cggcaactta cgatgatgtt
     7021 tacaacccgt atgaagaccg tcaggatttc ttcggagatg tgagagtgcg ccaggcgatc
     7081 gcctactgtg tggaccgcga gtacatccaa acgtatatca ccctgaacca atcggtcatc
     7141 cccagcactt tcctgccgcc caatcatcct ttcgcggcgg cagagacgcg gagctacacg
     7201 cacaaccccc agttgggcat ccagctgctc gaagaagcag gctggcagga tagcgacggc
     7261 aaccactcaa cccccagggt ggcggcagat attaagaata tcttctacaa caccgaacta
     7321 tctctgaatt actacgttac cgagagtgaa ctgcacgcgg ctgtgaccga agcggtggtg
     7381 aaatcgctgg gtgagtgcgg catcaaggtc acgcccacat tcctttctgt gggcgaaatg
     7441 ttcgcgcctg gccccgaagg gcggatcttt ggccgcaatt ttgacctggc ggagctggcg
     7501 tggtcaactg gtaggcagcc gccctgtttc ctgttctctt cttcagagat cccctcagca
     7561 aagaacaagt ggttgggcac gcgctatgga ggggtcaacc tcagcggata caccagcagc
     7621 gaatacgacg ctgcctgtga acaggcgctt tcagccgggt tggaccgcga tttgctcacc
     7681 gcccaatccg ctcacgcgca acagatcctg atggaagacc tgcccgcgct gcccctgttc
     7741 tttcacgtca aagccatggt ttcacgacct gacctgtgcg ggttgcaact ggatgtgagc
     7801 gcccgcagcc ctttgaagaa cattgaagct ctcacgctcg gagagacctg cccgggcaaa
     7861 tgagagggca ggcgggagac gccattccaa caggttttgt gttagaatct accccactgc
     7921 ggatggagtg tattggggcg tggtgcaatg gcagcacacc agactttgga tctgtggatc
     7981 ttggttcgaa tccaggcgcc ccagcctgtg cagtaagtga atgaaaaaga aatcagaacc
     8041 tcccagatct caatccgtgt ttttcggctc agctgccaca agttggatgg cgcgcggctg
     8101 tgtttaatcc ggtgtagaat aaaatgactc gctggtatct ttcgtggaga gaaatatgaa
     8161 tctttctacc atcatcctcg ctgccgggca gggaacgcgt atgaactcaa ccctgcctaa
     8221 agtgctgcat cctttaaatg ggaaactgct tgtaaaatat tccatcgatc tggcggacgc
     8281 gctctcttcc agtagaacag tggtggtgat tggaaacggc gctgagcagg tccgcagtgc
     8341 tctgggagaa tctgtagcct acgcgctgca gccgcagcgc ctgggtaccg ccaacgcggt
     8401 gcaggcggct gaagggttgt tggctgacgc cagtgggttg gtgcttgtca tttcagcgga
     8461 catgccgctg ctcaccgcgg ataccctaca gaggctggtc accacacaat cacaaaacac
     8521 cggcccaatg gccctgctca ccgtgcgctc agccaatcca cgcggctttg ggcgcatcct
     8581 ccgcggcgtg gatggaggag tgacaggcat tgttgaagaa gcccaggcca cggctgagca
     8641 attgaaaatt gatgaattaa atgtcggcgc ttattgtttc aattcttcct ggttgtggca
     8701 ggcgctgcgg aagatccagc tttcaccgaa gggtgaatat tacctcacgg acatcgtagc
     8761 cgtggctgtg gcagaaggtt cgcctgtttc cagccttaca gtgacagacg aggaagaaac
     8821 ccttggcatc aacaaccgcg tgcacctggc ggaagccgag cgcgtcatcc gacgacgcat
     8881 caatcaagcg catatgctcg ccggcgtaag catgactgac cctgaacttg tttatataga
     8941 agaaagcgtg aaaattggtc gagacaccat tctgctgccc aacacctggc ttagcggcag
     9001 caccatcatc ggcgaaggct gcgaaattgg accgaacacc ttcatcaatg actccacaat
     9061 tggcaatcac tgccacctgc tggcgtcggt ggttgaaggc gcggtagtag aagacgaggt
     9121 tgggatgggt ccgttctgcc acctgcgcaa aggggcgcac ctggggcgtg gtgtgcacat
     9181 gggtaatttc ggtgaggtga aagactctta cctggcggca ggggtcaaga tgggtcattt
     9241 ttcgtacatt ggcaacgcca cgatcggcga aaacgtcaat atcggcgcgg gcaccatcac
     9301 ctgtaatttt gacggcgtga acaagaaccc caccgaaatt ggtgaaaaca ccttcattgg
     9361 ctccgatacc atgctggtag cccccgtggt gattgggaaa aacgccaaaa ccgccgccgg
     9421 cgcggtggtg acgcatgatg tcgccgacaa tgaagttgtt gcgggtgttc ctgcccgccc
     9481 aatgaagaaa aaggaatgaa gtgaccttga tcgcctggtt gttacttggc gtgtttgtcg
     9541 cgctggatat ttttatctgc atcatacggg cggcgctttt taatgcgcgc ctgccgcagc
     9601 tgatcgacct gggcacagaa gaccgggaag aactggagcg caccatcaag gtgctggaaa
     9661 ccacccggct gcgcgcaaca atgcgcttat tggtagccgt gctgcacgcc ctggtactcg
     9721 cctccctctg gttattactg aaaaccacat atacccagct ttcgatcggc ggcttgttgg
     9781 gcatcttcgc cttagccata ttcacgctgc tctgcttcga attcctggca gagcgttttc
     9841 cactgaaaga ccccgaaacc tgggcgatgc gcctggcgcc gctggcgaat tttcttgacc
     9901 gacttttcag cccgttcacc tcgctgctgg tgggtctgca gggctccggt gatacggtac
     9961 agcgcaacct gggggctgtt tcagaagatg agctgaagac ctgggtggag gttggcgaac
    10021 cagaaggcgg gctggaacct gatgaacgca agatgattta ctccatcttc cagtttggtg
    10081 atacgctttg ccgtgaaata atggtgccgc gcattgaagt actggcgttg gatgtggaca
    10141 cgccattgaa cgacgccatt aatcaattca tcaccacagg gcattctcgc gcgccagtgt
    10201 acgaagaggt gattgataat gtgatcgggt tgctctacgc caaagacctg ttgaaactgc
    10261 ttacttcacc agccaagaat cagaccatcc gcaaattcct gcgcccggcc tattttgtgc
    10321 ctgaatcaaa aaaagcggat gagctgctgg cggaaatgca ggccaggggg attcacctgg
    10381 cggtggtggt ggatgaatac ggcggcatgg cgggattggt aacccttgaa gatatcgtcg
    10441 aagaaattgt gggcgagatc cgtgacgagt acgatcaaag cgaagaacgg ctggtagaaa
    10501 agatatcgga taacgagttt gtcttcctgg gcagggtttc gttagatgac tttaatgaag
    10561 cgctggatac tgaactggag tctgaccaaa cggatacgct gggagggtac ttctacagcc
    10621 aggaaggcag ggtacccgtt gaaggggacg agctggtctt tgatggctgg cgtttaacgg
    10681 tggaaggagt gcatcaccgc cgggttggcc ggatacgcgc ggtgaagatg gagaaacagg
    10741 atgaagacaa cgacacaaat tgatggggag atgctcgaaa agttaatcga aaccgcccga
    10801 aacgcgcgca attgggctta cgcgccttat tctcactatg cggttggcgc agcgctgctc
    10861 accgccagcg gtaaaatcta cgatggcgtc aacatagaaa atgctgcttt cccggcaggt
    10921 atctgcgccg agcgagtggc gatgttcaaa gccgtttcag agggagagcg tgatttcact
    10981 tttctcgtgg tgatgaccga taacggcggt tcaccctgtg gttcctgccg ccaggtgatg
    11041 gctgagtttg gtctttctac ccgtgtgctg attgtggata ccaacggcaa aacgcatata
    11101 gataccactg ttgaggggtt attaccctgg gcgttcaggc ctgaagacct gctttgaggt
    11161 tctatgccca ggagcgagcg caatatccgg gtggaggcgg tggtgctgcg ccattcagat
    11221 tggggcgaag ctgaccggct gctggtaatc tattcgcgtg aacagggcaa actacgcgcc
    11281 atcgccaaag ggctgcgcaa aatccaatcg cgtaaagccg gtcacctgga accatttacc
    11341 cgcgctgcca tcatgctggc aaagggacac gacctgtgga ttgtgaccca ggcagaagcc
    11401 atcgaccttt ttcaaccctt gcgtgaaaac ctgcaaaaga tggggcaagc ggcttatgtg
    11461 gtggaacttc ttgatcgttt cacatatgaa gagggacaaa actggcaact gtaccagctc
    11521 ctgaccgaaa cacttaccag actgtccacc acagaagaag actttgtgcc ggtgaaatat
    11581 tatgaaatgc gcctgctcga cctgttaggt taccgaccat tgctgtttga gtgtgccgcc
    11641 tgcgccgaag cgatcaccgc ccgcgaccag tacttttcag cggatatggg cggtgtgctc
    11701 tgccccaaat gcgccacgcg cggtcagagc agccgtccgg tttcgatgga agcgctgcgt
    11761 tttttgcgtc attttcaacg cagcaattac aaagaagcca tgcgcgccgc tccgccgcct
    11821 gccattcgcc aggaaatcga tgccctgcag cattactatc tcacctattt actcgaacgc
    11881 agcttgaact cgccagaatt ccttaaacaa attcgcaagc aaatgtgacc ccgcccgcca
    11941 ccagcggcgc cccttgctct cggggtataa ttggaacact tcaaagtcac accttcccgg
    12001 agaattaatg agcaaagcat ttgcgtttca atccgtgatc atgtcgttgc agaaattctg
    12061 ggcagaccag gggtgcctga tttggcagcc atattacagc caggtgggcg ccggtacgat
    12121 gaacccggcg accttcttgc gcgtgctggg cccggagccc tggaaagtgg cgtatgtgga
    12181 accatccatc aggccggatg acggacgtta tggggagaac ccgaaccgct ttcagcagca
    12241 ttaccagttc caggtgattc tcaaacctga tccgggtaac ccgcaagaaa tctacctgga
    12301 atcattaaag gctatcggga ttgacccgag agaacacgat atccgctttg tggaagacaa
    12361 ctggcagcaa ccggcgctgg gcgcctgggg gctgggctgg gaagtttggc tggatggtca
    12421 ggaaattacc cagttcacct attttcagca agcgggtggg ttgatgctcg acccggtctc
    12481 cgtggagatc acctacgggc tcgagcgcat taccatgccc ttacagcgtg tgcaccattt
    12541 ccgcgagatg cgctggaacg acaccctcac ggatggtgat gtgaacctgc aggctgaagt
    12601 ggagcatagt aaatattact ttgaagtggc cgatgtggag cgcatgcgcc agctctacca
    12661 gctgtatcag gcagaagccg cagaagcgct ggggaaggga ttggtgttgc ctgcttacga
    12721 ttacctgctg aaatgctcac acaccttcaa tatattggat acccgcggcg cgatgggtgt
    12781 gaccgagcgc caggcgatgt ttggcaagat gcgcgagatg gcacgtgcga tctcggaggc
    12841 ttatgttgcc cagcgtcagg aattaggctt tccctggatg agaggtgaaa aaaagacatc
    12901 ggctgtagag gtgaagccaa tagaaaccca tgttttcccc gatgcaccca ccgatctatt
    12961 attggaaatt ggcaccgaag aactgccggt agctgacctg cagagcgcga ttgagcagtt
    13021 gattgaacgc gtaccgttga tgctggatga attgaggttg acccatgggc aggtgtctgt
    13081 ggcggggacg cctcgccggc tggtggtaat agtggaggac ctggcggcgc gccaggaagc
    13141 caaagaaacc ctggtcaaag gtccaccagc cagccgtggt tatgatgcca atggattacc
    13201 cacaaaggca gctgaaggct ttgcccaaag caagggcgtg agggtgcaag acctgcgcgc
    13261 gcgcgagatt gacgggggta cctacctggt agccctggta aaggaagata cccgcccaac
    13321 tcaccaggta ctgggcgaag cgcttccagg gttgctggaa agtattaagt tcgaaaagac
    13381 catgcgctgg aatcatacca atgtgccatt ttcgcgcccg gtgcgctggt tgaccgcgct
    13441 cttaggcggt tctaccattc cttttgaatt cgctggtttg cgggccacga ataccacgca
    13501 tggcttgcgg tttaatgaac ctactgaaat aactctcacc tctctggcgg attatcaagc
    13561 ctttctttcg agccagggaa tcattctcga cccactacgg cggaaaaaga cgatccagca
    13621 gcaggtgaat gagcgctgtg aacaggtggg cggcaggcca ttactggaag aagaactgtt
    13681 ggaggaagtg agccggctgg tagaagcccc cacagcgcta ttgggccggt ttgaccccgc
    13741 gcatctcgag ttgccaccgg aagtcctcat ttcagtgatg aagaaacatc agcgatactt
    13801 cccggtgtgc aatgacgcgg gcaaattact gccgttcttt gttgttgtgc gtaatgggga
    13861 tggtcacggc gcggacgtgg tcacagatgg gaatgagcag gtcatcaagg cgcgttttgc
    13921 ggacgcgcag ttcttcatta aggaagatat gaaacacaaa cttgaagaca tgctggttcg
    13981 cctgggctcg ctcacgtttc agcataagct gggctcaatg ctggacaaat ctcgccgggt
    14041 tacccttctg gcagaccccc tgtcaggcgc gttgggtctc tccgcggcgg aaaagttgac
    14101 cgctttgcgc gccgctgaat tatgcaaggc cgacctggtc acgcacatgg tggtggaaat
    14161 gacctcgctg cagggcatca tggggcagta ttatgcgctg cattctggag aaacgcaggc
    14221 tgtcgctgat gccatccgag agcactacct tccacgctac accggagaca gtcttcccgg
    14281 ctcaaaggca ggactggtcg ttggattggc tgaccggttg gattcgctgg tgggtctctt
    14341 tggcgctggt ttggcaccta ccggcacccg tgatcccttc gcgcagcgcc gcaccgccat
    14401 tgggcttgtt cagttactga cggcgtttga tattgaattt gacctgcgcc aatggttgga
    14461 actggctgcc caaaacctgc cgctcacgat tagcgtggaa aacatgcaga cctgcgctga
    14521 tttcatcacc gctcgcctgc gcaatatctt aattgagcag ggcgcgcgtt atgatgtggt
    14581 ggatgcggtt ctggcggaac aagccaataa ccccgccagc gcgcagcgcg gggtgaagga
    14641 actggggaat tgggttgaac gggaggattg gaacagcatc ctgccggcat atgcccgctg
    14701 tgttcgcata acccgggatc agaagcagat ctatccggtt aatccggcgg cgttcgcaga
    14761 ccccacagag gaagctctgc acgaagcgtt ggtgaaggct gaacaaacac cacgccgcgc
    14821 gggttctgtc agggattcgc tgaatgtttt cctacctatg atcccggccg tcaacgcctt
    14881 ttttgattct gtactggtaa tggctgaagg ccctggtttg cgcaccaacc gcttagggct
    14941 gttgcagcgt gtttccgcaa taatgcgcgg agttgctgac ctgtcgaagt tggagggctt
    15001 ttagtcattc gctcaacaga gccgaaccgt tttctgtact acaattgatc ctatgaccgc
    15061 gattgatgag ataaaagcca ggctggacat cgtggacatc gtatccgaaa cggtgaaatt
    15121 gcgccgttcc ggcaggagtt attctgggtt ttgccctttc cacccaaatt ctaaaacccc
    15181 ggctttcgcg gttttccctg ataatggcac ctggcattgc tttggcgcct gcaatgaagg
    15241 cggcgatata ttcaaattcg tgatgaagcg tgaaggctgg gattttgccg aaaccctcaa
    15301 atatttggcg gaaagggcgg gcgtgaccct gacgcccatg acgcctgaac gcaaggaaaa
    15361 agaagaccag tacgaggtta tgcgccagct cctcgaagaa agcgttaaat actaccaatt
    15421 tcaattaacg aagacacctg cggggcgagc ggcgtatgat tacttgatac aacgcggtct
    15481 tacacccgcg tccatagaag cctttgaact tgggtatgcc ccgcaatctt atgaagcgct
    15541 gctgggtcat ctgagcggca aggggcacgc gcttgagcaa atgctggaag tggggctgct
    15601 ggtggagaag gactctggcg gttattatga taaattccgc aatcgtatca tgttccccat
    15661 tcgcgatggc gccggaaaga tggccggctt tggcgcgcgg gtgcttaacc ccgaggatgt
    15721 tcccaaattt ctaaactcgc cgcagacccc tttgttcaac aaagggcggc tgctctatgg
    15781 tttgaacaag gcgaaaaggg ctattcgtga tgccgaccag gtggtgctgg ttgagggcta
    15841 cctggatgtg atcgccctgc accaaaacgg ttttaccaac agtgtttctc cgatgggcac
    15901 agcgctcaac gaggatcaat tgcgctacct gaagaaatat acccggcgca tcgttcttgc
    15961 gctggatgcc gatgcggcgg gcgagaaagc cacactgcgc ggtgttgaaa ttgcccgcca
    16021 ggcgcttgac cgtgaagccg aattgactta tgaccctcgc ggtttgttga agcatgaagc
    16081 ccggctgcag gctgatgtgc gtgtgaccac cctgcccccg gggttggatc cagatgatgt
    16141 ggtgcttaag gacaggcagg cctggcagca ggtgctggaa agcgccaagc ctatcgtggt
    16201 tcacgtgatg gaaacgctgg cggcgggtca aaaccttgaa gatgccaaag tgaaatctgc
    16261 catcgcggcg caggtgaccc ctttgatcga agatgtccct tccgccgtcg aaagggatac
    16321 ttaccggcaa cagctggcgc gccttttgaa agttgatgaa cgttcattaa tgggcctgca
    16381 aaaggttcgt gggcgaactc gccgcgccag cgcagaagcg gcgcaaggca ctcagatggc
    16441 aagatcaacg cctggcgcga gaactgtgga tagccgaacc ctgcaaatgg agaaacacat
    16501 tttatcgctc ctgctgaacg atactgaccg ttattacgag ttggagaggc gtataaagtc
    16561 ggcgggggtt gaacctcttt cggttgcaga ttttgatgac gcgcaaaatc atgaattgat
    16621 caagctgctg atgaaagcca tgcggcagga tgctcaagag gccctggatt acctgagaga
    16681 gaacaccacg ggtgatttta ctgaacgact ggatgaactg ctctcagaaa cgaacattaa
    16741 aaacacgcaa accgaaacga tcctgtctga cctgtaccgc acgctcatct cgttacgcct
    16801 gatccgcgta aaccaggtca tcaaccagct gcgcttcctc caggaagagg agagcgcaga
    16861 ccgcgacgac caggcattac agtccatgat tttgagtcac acgattgctc ggggaaaatt
    16921 ggaccttgcc ctggctaaaa cgcaataaaa aagagacaaa acacttatgg tatagttaac
    16981 ccatgtcctc cggtcaaaaa aaatccgcac aaagcccttc gtttcagcag gatgctcctg
    17041 ctgaagtaaa cgcggtggtg aagacggctg tcaaacctca catgaaagca aaaaaaccac
    17101 aacgcccatc agcgcgcgcg aaaattgaac acaaaaccat cggcgcagcg ggcaaaccag
    17161 gttctcaacc gggcagccaa aaagttgtga atgctacggt gaaaacccct gaaattcaag
    17221 atacagaaga acccggaacc gcgatgggag cggctgaatg gaacgagatt gagattgaag
    17281 acccggctga gatccttgaa gacccggcta tcgcgctgga gctgaccgaa gatcctgtcc
    17341 gcctgtatct gaaagaaatc ggtcaaatca acctgctgga cgcggatgct gaattcaggc
    17401 tggcagccag gatcgaagcc tgtgtgagag taaattctgt gcgtcaacac gtccgaccct
    17461 ccccgggaaa agaaaaacac taccgcgccc tgtttaccgc cctgctttca gaattgacga
    17521 cttcgtggag gcgtttgcag gaggacacag aacgtatcgg gttgatcgaa tgtcctgatt
    17581 tcgcgctgac cctcgctgag gcgcagctcc tcaggcactc gtggcaatcg gacgaaccct
    17641 cctacctgcg caaccacctg gaccacgcgc tttggggcaa agaccccgtt tgggaaggac
    17701 tggtcaggca ggcttttacg gtttttatat gcatgtatct gttgccagaa cgagtatcga
    17761 acgctctatt tgaatatgtc atccagcacg acaccattcc tgatatggcc tggctgagaa
    17821 gacgattacc ggatgaaaaa gcgctggaaa ccgaggttga aggggtcttc aaccgggctg
    17881 aagaagccaa tcagactttg attcgagcca atttacggct ggtggtgagc atcgccaagc
    17941 gctaccttgg gcggggtatt tcgtttctcg atttgattca agaaggcaac ctgggtttac
    18001 tgcgcgcggt gacgaaattt gattctaccc gtggatttaa attcagcacc tacgcgacct
    18061 ggtggatcag gcagtccatc agccgttata tcgctgagca cgcccgcacg atccgtatac
    18121 cggtgcacct cttcgaagcc attacgcgta ttttacgggt gcagcgcagc ctggtgcagg
    18181 agcttggcag ggaaccaacg cttgaagagc tggctcttga atctgacatt atctcaccgg
    18241 aagacgcagc ggcaatcaaa atggcacggt ttaccaatca gccgcttgag ggagaaatgc
    18301 agcggcgctg gtcctgggcg actttgaagg tgcagcgcat tcttcaatcc gccgaggaac
    18361 cgatctcttt ggaacggcca gtgggtgatg aagaaagcag ccaactgggg gatttcattg
    18421 aagatgatga ggcgttggaa cccatggatg cggcagcccg cgagatgttg cgcgaacagg
    18481 tgcaaaacgc gctggctgcc ttatcagagc gtgaacgtca ggtgctggaa ctgcggtttg
    18541 ggttgatcga tgggaaagac cacacgctgg aagaagtgag ccgttatttc aacgtgacaa
    18601 gagaacgcat acgccagata gaagccaaag ccttgcgtaa actgcgccat cccacacgca
    18661 gccgttttct aaaagaatac ctgggataac aaacaccctg gcagcagtta attgcaagtt
    18721 tcgctcagtg cttcgcgcag gctgatgatc tgccaatccg ttttcataag ttgctgcaac
    18781 tcatccaggt tttccgggat gatgtgaagg atcagaatgc cacccggttt gagcggcatc
    18841 cggtttggct caaaagtgtg ctcgtcagcg ctccagtaca ctggggtgaa gccctgcttg
    18901 tgcgctgcct gcatgaactc tgccgtccac cttccccagg gagcgcgtac ataaggggtt
    18961 acaccgcgag agtagaggtc atccccgatg accgccctta gcgcatccga ccaatcctgg
    19021 taatcttgca gccattcttc ctgcgacatg ccgtcaatga tattgacgtc aggatgggtg
    19081 agactgtggt aaccaatatc gtgaccgtta tccaccaggc gttggaaagt atcccgctgg
    19141 atattcttgc caaaagaggt gcccacaagg aaaaatgtcg ctcgcgcacc gtgctcttcc
    19201 aggatgtcca gcatctggtt gagcacttcg ttattgtagc catcgtcgat ggttaagacg
    19261 ataaatttat caggggtatc aaacgatttc aacaggccgt tttgatcaaa taccgggatg
    19321 gctttcacgc ggtttatctg gcaggccgcg ctggttggtg tgaaggttgg ttcaaccggc
    19381 gcgggtatct ccacagtggc cgtggctggc ggcgtggagg tgagcgtggg tataggtgtt
    19441 gaaggcgggt ctacaatcag tggggcagcg gccaaccgtg gggcgcaaga cgaaagcaga
    19501 atggcaaaac acaaacctac caataaacca acgttaaaga atgaacgcat tgacactcgc
    19561 tttgaatgaa tttgacaaca gggtaatttt actcaatacc cttgacttgc gtcagttttt
    19621 tttccatgat atactcattt tacttgagta atcacaataa tgaaaaggtt atttccggct
    19681 tcacgagtga atccggaaga caaaaaatcc cgtcactaaa aaacctatca gctttcccga
    19741 gcctgacccc aggtcggggt gccaatctcc aacctatact gatggacatg aacctatgaa
    19801 aataattgaa atggaaaaag cgtccctatc ctcgctgcgc gcgattgcgg aagagctcaa
    19861 agtccccaac gcaaaaagac tgaaaaaaga aaatctgatc atcaatattc gcagggcgga
    19921 agccgagcaa gaagggcttg aagtgcgcgg cggcgtgctt gagatcaccc cggaaggcgt
    19981 gggtttctta cgcaccaatt attcaatcgg tgcggaagat gtttacgtct cacaggcgca
    20041 gctgcggcgg catgaactgc gcatgggaga cctggtcatt ggcgtggtga gaccgccgcg
    20101 agacgccgag cggcattacg gcttgcttaa ggtggagagt gttaacggtc tcgccccgga
    20161 tgaaatgcgc aaccggaaga cctttgagag ccttactccc atctttccag acaagcgctt
    20221 tgacctggaa accgataaga gcattctttc ttcgcgactc atcaacttga tcgctcctat
    20281 tggacgtggt cagcgtggaa tgattgtatc gcccccaaag gcgggaaaaa cgaccatcct
    20341 taaacagctg gccaactcta tcaccgctaa caaccccgag atacacctga tcatcgccct
    20401 tatcggtgaa cgtcctgaag aggtaactga tatggaccgc tcggtggacg cagaagtagt
    20461 ggcttcaact tttgatgaac cggtcagcgc tcatgtgcgc acagctgaaa ttacgctgga
    20521 acgcgccaaa cgcctggtgg aaattggccg cgacgtggtc atcttgatgg attctctcac
    20581 ccgcctggcg cgcgcttata acctggtcgt caatccgtcg ggtcgtacac tctcaggtgg
    20641 aatggacccc tcggcgcttt acccaccaaa aaaattcttc ggtgctgcgc gcaatatcga
    20701 agaaggaggt tccctcacca tcatcgctac cgcgctggta gataccggct cgcgattgga
    20761 tgatgtggtt tacgaagagt tcaaaggcac cggcaacatg gagctgcatt tatcgcgcag
    20821 gctgcaggaa cggcgcgtct tcccggccat tgatattgaa cgctcatcca cccgccgtga
    20881 agacctgctg ttgggaccag atattctgcc gcgcgtgtgg ttgatgcggc gtatgtacct
    20941 gcagatgatc gccaccccgc cgcagggcgc gggtatggac ccggcagtgg caaccgaagc
    21001 gatcttacag cgtatggcgc gcaccaaaga caatatggaa ttcctggaga atttaacgga
    21061 ggattaattg acaacgaaaa aaacagcccc caaggtggct gtagagccgg aagaacagac
    21121 gacgcctgta aaacctgctc aaccagcgcc ttcctggact gtcattctct catgggtggg
    21181 aactcttttc ctggtggcaa tcttgctggc agtgctgttt aggtttaacc ctgctgatag
    21241 agagaaaaag agccatgaac ctttgaacgt agcctctgac gcgcaggtgg aactaccggc
    21301 tttaacttcg gcccaggcag actattcttt ggtgagagcc gccagcctgg acacgaccat
    21361 acctgaaggg gtgcgccatt tcgccatcaa gcacaccgtt gaagagggtg attcaatttt
    21421 cgccattgcc aaaaaataca acctaaaacc ggaatcaatt ttgtgggcga attatgacct
    21481 gctgcatgac gaccctacgt atctaaatcc cggctggcag ttgaccatcc cacccacgga
    21541 tggcatctac tataagttga aggagggaga caccctagag aaattggcag agaagtacta
    21601 cgcgaatgtt gatgacatta tcagctggcc atctaaccat atggatatta ccaccccatc
    21661 tgtcgcgggg ttggaatatg tgatgattcc cggcggatat cgtgaaatcc aatcctggat
    21721 cgtgcccctg gcatacacct ctggttcagg cgcaacgcgc gagatcgccg ggccgggcgg
    21781 gtgcggttca accaattatg tgcttatcgg ttcaactgcg ttcatatggc cggctgtgca
    21841 atatgacctt tcagggtttg atttctccag ttaccacctc gcgattgata ttgccgctgg
    21901 tgtaggagat gcggtctacg cgtctgatag tggcacagtg gtgtacgctg gttggaatga
    21961 ctcaggttat gggaacctgg tggtgatcga tcacggtaac ggctacgaaa cgctttacgc
    22021 ccacctttcg caggtatatg tctcttgcgg tcagggagtt tacagagggg gtgttatcgg
    22081 cgccgcggga aataccggtc gctccttcgg ttcacacctt cattttgaag tgcgcttgaa
    22141 tggcggtttt gtcaatccct ggtcggtgtt gggttattag attataaaaa aaaacaagtg
    22201 aggcgggagg gatttccctc ccgctgggct tttaaagaag gttcaatggg tcgagattca
    22261 ggtctaccag caacccgggg tccttccaat caggaaaagt gtgttgctca agcaccacgc
    22321 gcgggtcagg gccgcggatg atcacctgcc agcggtaata accagcgcgt ttctgataaa
    22381 agcagggcac tggcccgatg agcgaggtgt ttcggtagtt ctctgtgtca atccagtgtt
    22441 tgagctgtcc gccggcggtt tgcgcgctgt gttgtgcttt ggccgcgtct gtgtgcctga
    22501 attcaatttt taaaaggcgg gaaaatggcg gatacccggt gcgctctcgg taggccaact
    22561 cggttctgta gaacccctcc gcgtcataag ccgcggcagc ctggatggcg taatgcccgg
    22621 gttggaaggt ttgaaaaatg actttgccgc cgcgagcgct gcgccccgcc ctgcctgcta
    22681 cctgggtgag caactgatag gtgcgttctg ccgcgcgaaa atcaggaaga ttgaggctga
    22741 tatctgccag aaccacccct accagggtca ccagcggcag gtcaagcccc ttggcgagca
    22801 tttgtgtacc gataagcacg tctgcccggt gctggataaa atggtccaga atgagatcgt
    22861 gggctccccg gtaacgggca gtgtcggcgt cccagcgcag caggcgcgcg gatgggaatt
    22921 ggccctgtaa atacttctcc aggctctctg ttcccatccc aaattggcgg atgttcatcc
    22981 cgccacagga agggcattgc gaaggcagcg cgcggcggta attgcaggta tggcaaatca
    23041 actggttttc atcagcgtgc caggtaagct gagtttcaca acgcggacag cgtaatacaa
    23101 aaccgcaatc acggcaaaaa acatacgagg cgctgccgcg gcggttgagg aaaaggatcg
    23161 cctgttcatt acgctgcagc acttcctcca gtgcggtgtg taacgattgg ctgatggctg
    23221 agcgattgcc agcgcttaat tccttccgca tatccaccac ttcaacctgc ggcagatcgg
    23281 taaagttcgg ctgggtggat ggtggttgtt gaccgtccgg cgaggtatgt gccaaaattc
    23341 gctggggaag gtggtgtatg ggccagtggt tctctgtaaa ccggtaaagc gtttccacat
    23401 ctggcgtggc gctgcccagt aacagacagg cgccgttgat ttgactgagc gaaagcgcgc
    23461 attgcacggt gtgataatgc ggttgcgtgt cttcctggcg gtaagaggga tcgtgcgctt
    23521 catcgatgac gatcaacccg gggtcggaga gcggggaaaa gagcgcgctg cgcggaccga
    23581 tgacaatggg cagcaagcca gcgcggatac gacgccaggt atcgtagcgc tcgccaggcg
    23641 aaagcttgga atggatcaga ccaaccttcc cagggaagcg cgcgaaaaag cgcttcaccg
    23701 tttgcggggt gagcgaaatc tccgggacga ggatgaaaac ccctcgccct ttttccacca
    23761 cagcggcagc agcccgcagg taaagctcgg tctttcctga tccggtgacg ccaagcagca
    23821 ggttgggtac gggcgtggat gggttcacca gctgatgatg aatctcctcc caaacctttt
    23881 gttgatgatt ggtgagcaca ggtggatgga tgagcacggg ttgtagtttt tccagcgggt
    23941 cgcgccagat ttccgtctca ccgagaatga taagccccat ctcggcaagg cggttaaggt
    24001 ccgcggagga agctccgctc tccgcataaa cccacgaaat atttaccggg attgcctgcc
    24061 gctcgaggaa ctcaagcgcg gcaacacggc gctgggctgc ttgtgagctt gcccgaccga
    24121 caagaatacc aggatcatcg cgccatcctc gcggggcgct gaacagcgcg gtgcgcacga
    24181 cctttgggcg gacagacggc gagggcagaa cggattgagt gactaccagc tgcgccttaa
    24241 ccaatgctga caggctctca cgccaattca cccggggcag cgccgtgtct acctggcgcc
    24301 cgcgcaaggg gccgcgcttt tccagcagat caagcaggcg ctgctgcaca gatgagagtg
    24361 cggttgttcg cgcttgtttg ctgcgcgtta ccagagtgtc agcgtgctgg ctcagcccgg
    24421 gcggcaccat caggtcaagg cattgcggca gggtggccag gttatcacgc gccatctgtt
    24481 cagccaggcg catttgccag gaagtcagta ctggtgtatc gtcaacaaca gtcgcgatgg
    24541 gacgggtttg atcaacagcc ggggtttcaa gcagagagat cacaaccccc tgcgcggttt
    24601 gcctgccaaa tggcacggtg accaagctgc ccggttgaat atggggcgcg tactcatcag
    24661 gcacctggta atcaaacaga ccggttaatg gagcgatgtt gacagaaaca cgggcgtaat
    24721 tggacattga aaaacttagc gggtgaccgc gagtgcgcca taaccgacca cgcggctggc
    24781 atcgcctgag gaatcagcag aggtactgtg gtgcagcagc gtaaccctgg tagcgccgag
    24841 ctctttttcc gtccacaaca tcaaagccac cgcacctgcg ccgcaggcaa acccttcgcc
    24901 gcagcgctcc gctctcagca caccctcagg tgaaaaagcg ctgatttgtt gcagcatgct
    24961 ggtgtccagt tgaattgctt cactctgagt atagaaatgt gaaaggtcgg tactggctac
    25021 cagcagcgca ttctgttctt tgagtgcagg agcgattgcc gccgccgcag ccgttaaaag
    25081 agccgggtcc tgactgcgca tcatgattgg taccaggcta aagggtgcgc ttaacgccac
    25141 ctgcaggaag ggaagctgga tttcaagcga atgctcacgg tcattggcga tgggctgcag
    25201 gatttcgccc gttgctttgg cgatgttcgc agaacactgt tctaccagat gctggttaat
    25261 gggcacctcg ccaagggggg tggcataggc aaggtgagag ctggtgagca gctggctggg
    25321 caggtaatca tgcaggggtg agaaaacaac acaaacatca aacgcgcgcc ctttgacaca
    25381 acggtaggca tgccctgcgg ttgcgccgga ataaaaatac ccggcgtgag gagcgataag
    25441 acctgtgact tcacccggca gcgctggcgg tgttgctgcg tcaatgtacg ctttgatctc
    25501 attacgcagt atatgcgggt tgtctgagta ccaggcaccg gcgattggcg aacggcggat
    25561 ggggggatgg gggttcatac acacctcgct gtctatatta tacaatcgcg cgccataaaa
    25621 aaaccggtca cgtattggtg accgggaagg gaggtgagta ccattattgg tcatcgcgtt
    25681 cgcgcaaagg tgggaagagc agtacctcgc ggatattttt ctgatcggtc aacagcatca
    25741 ccaggcggtc gatacccatg ccgaacccgc cctggggcgg catgccataa gacatggcct
    25801 taaggtaatc ttcatccatg gggtgctgtt cttcatcgtc cgctttgtaa tcacggccca
    25861 tttcgagaaa acgcttttct tgctccagcg ggtcgtttag ttctgtaaat gcgttgcaca
    25921 gttccattcc gcacacaaaa ccctcaaagc gttccacggt cgatgggtca cccggcaggt
    25981 tcttggccaa tggggaaata tctcgtgggt aatcaatcat aaaggtcggc tgcaccaggg
    26041 tgggctctac aaattcttcc agcagctgtt cgataagctt gccccgagct tccccgggtt
    26101 tgaccttcat gtcaagtgaa accatcattt cttcaagtgc ttcggcggtg gggtaaagtg
    26161 agatgtcaat attggctttc tctatcaaac tttggcgcag gttcacccgt tgccagggcg
    26221 ggttgaaatc cagttctacg ccgtcgtaag tcaatttgcg ggtgccaaaa accgaatcgc
    26281 aaacataggc gatcatctcc tcagtcattt ccattacctt gaggtagtct gcgtaggccc
    26341 aataaaactc cagttgggta aactctgggt tatgcttata agacactcct tcattgcgga
    26401 agtcgcggcc gatttcatac acccggtcaa accctcccac ggtaaggcgc ttgaggtaaa
    26461 gctcaaaaga gatgcgcagg taaagatcct gctttaactg gttatgatgc gtaacgaagg
    26521 gcttggctgc tgctccgcca tagataggct gtaagatggg ggtttcaacc tccagaaaac
    26581 cacggctgtc gaggaactgg cgcagcgcgc gcaccacggc tgcgcgcgtg cggaagatct
    26641 ggcgaacctc ctggttcaca gccaggtccg catagcgctg acggtaacgg gtttcgggat
    26701 ctgaaagggt ggcatggcgc accacctgcc catccaccac ttcatcttta gccgcgggca
    26761 ggggggtgat ggctttcgcc agcattttaa agttgcgcac cagcaggctt acctctcctg
    26821 tacgcgtacg caccaggtct ccgccggctt gaatgaaatc acccaggtca aagtggtcct
    26881 taaaccgctg catttcggct tcccccagtt cgtttacccg gaagaaaagt tgaatgcgac
    26941 cgctgccatc ttcaatgtgg gcgaatgcca gcttacccat caggcgcacc gcgcgcagcc
    27001 tgccgcccag cacggtggtc agcgtttctg gttttccagc cttctcggca ttttcaaagg
    27061 ctttgatcgc gtctgctgtt gtgctggtta cctcggtgcg cgttggatag ggttcgaaac
    27121 cgctatcgcg catttgctgg attttttcac accgtactct ttcaagctcg ttcagttcca
    27181 tggtttgttc cttttcaaat caagaagccc gcccttgttt gcggggcagg cctcggtttc
    27241 ttcagagaac gaatcgctct ggtgttgctg cggcgtagca cccggttgat tataggtcca
    27301 ccttgagcac tttgaccgtg taagcacctg cgggggcctc aatttggacc ttgtcaccca
    27361 cgcgtttacc caacagcgct ttccccattg gagattcgtt tgaaattttt ccgagggttg
    27421 ggtcagcttc tgctgcgccc acgataacgt actcttcttt ttgtgtcgcg cctatctcct
    27481 ggatagttac acggttaccg acctgaacgg tttctttatc aatctccgcc tcattcacaa
    27541 cacgagcggt ggcgagaaga atttctaatt ccttgatcga accctcaaca aaagcctgtt
    27601 cgtttttggc cgcttcaagc tcggcgttat ccagtaactc tccgccttca atggcttcgt
    27661 gcaatctttg ggcaatctgt tcgcgcctgg tagtgcgcag gtactcaagt tcatcctgta
    27721 gtttttgata tccttcggtc gtcaggactg gtgttggcat atccatttcc ttcccatatt
    27781 ggttcgtgat aggggaattc cctaagaaga tgaaaaataa tttagataga aaaaaaccac
    27841 tccggtggga gcgatttaat attgagagaa aattgtctct ttcctctctg aagtagccat
    27901 actataacat aaaaaaagaa cctttgcaag catt
//
LOCUS       DAOTLW010000027        27322 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_2078_length_27322_cov_9.886493, whole genome shotgun sequence.
ACCESSION   DAOTLW010000027 DAOTLW010000000
VERSION     DAOTLW010000027.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 27322)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 27322)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..27322
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2078_length_27322_cov_9.886493"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            39..1184
                     /locus_tag="P5516_05690"
     CDS             39..1184
                     /locus_tag="P5516_05690"
                     /inference="COORDINATES: protein motif:HMM:TIGR00200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family nicotinamide mononucleotide
                     deamidase-related protein"
                     /protein_id="HRT91964.1"
                     /translation="MTQAEIITIGTELLLGEILDTNTRYLAQQLRHLGIDLFRATSIG
                     DNPLRIAEMIRESLSRAEIVITTGGLGPTIDDPTRNAAAIAFNVEMDFHADLWKEIYR
                     KFQVRGAIPSDNNQKQAFLPANAVAIHNPVGTAPAFYIAEQKKLLVCLPGVPAEMKTI
                     FQHEVIPLLRGHFELHQLIKTHVIRTSGIGESVVDAQIADLETSSNPTVGLSAHPGCV
                     DIRITAKANSSQEADRMIDVVEQKVISLLQPHIYGFDQDTLLEKVIELAANRGVTVTL
                     FVFCAINELILFSNHTNNNVFEVKKLDRPYTPQQDLNLERRALLFAVNIYCQDDIATL
                     DMFFTSPQKHAEKKKLFNNALPQLQKWMENTCLDFIRRQLSEVENGE"
     gene            1186..3156
                     /locus_tag="P5516_05695"
     CDS             1186..3156
                     /locus_tag="P5516_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRT91965.1"
                     /translation="MKTVDILLRNAEVLTMDHEFRRYECGALAINGKIIEAVGDEKEI
                     LAQYTAPQIVDCAGKILMPGLVNAHTHVPMTLLRGLADDLRLDVWLMGYMMPVEREFV
                     SPDFVRLGTQLACVEMTRSGITTFADMYYFEEEVAKATAEFGLRALCAQTVLKFPTPD
                     AQFYEESLELSRDFIQRWKSHDLIIPSVAPHAPYTCTAEILKATAALAAEFDVPLHTH
                     LSETAVEVENMRRENRMPVIPYVKKQGLFEAKVLAAHCVHVDDGEMHTLLHVNAGVAH
                     NPSSNMKLSSGVAPITRMLEIGLNVGIGTDGPASNNDLDMFEEMRLASFLQKGFSGDP
                     TTLPARTTLLMATRLGAQAMHIGHLTGSLEQGKRADLILIDIEPLHNSPRFQHDPNGT
                     YAQIVYAAKATDVKDVMVNGKWLMRDQQIIGLDEEDLLKHSRDYSRKIDQFLIQRESS
                     VLSKLVAIGGTSEEESFEIQLKIRVDDRNKLIEKLLHSDMEILRKRHYREFDIYFFFA
                     DINQGILRYREDEFLNEMGKIEQVRSRLTLIGPTSEDKFEQEVLLSRSRYLAPATQSP
                     RFYREYFKPHLAKEIEKDRIRYLVKFKDVEFFINFDEITKPPIGQFVEVKSRTWSRQD
                     AEMKSHLITELISFLGANLDEVTSDDYIEMVR"
     gene            3192..4661
                     /locus_tag="P5516_05700"
     CDS             3192..4661
                     /locus_tag="P5516_05700"
                     /EC_number="2.4.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch synthase"
                     /protein_id="HRT91966.1"
                     /translation="MIRVLFIASEAAPLVKAGGLGDVAGALPAALNRLGAVVGGASAE
                     VRLVIPFYNSIKTSNISATFIRKFKVSTLNGAVEAGLFETSLSGVVTYLVSGAPVSES
                     ASIYGKDFKEDAEKFVFFSLACLKIPSLIGWKCDILHANDWHTAVAIHHLATAKKKNA
                     SLQTTRSVLTIHNLPFMGSGVDEALVKYKISPSNDDNLPNWGKTQPLPMGLAAADRIV
                     AVSPTYAKEILTPEYGCDLQDFLRKRSGHLTGILNGIDVESWDPATDPALKFNFAVSN
                     FTTRSQNKRALLAEFGFPVESDVPLLALVSRFDWQKGIDIIIAGLRAMKNFAWRAILL
                     GTGERNLEQACRDLEDEMPGKVRAAILFDVQLSRRIYAGADLLLMPSRYEPCGLSQMY
                     AMRYGCLPIARATGGLADTVSDPLSSTAPTGFLFPNADASSFITALKRALRLYKKRAF
                     WQAMQFNAMSRDFSWSVSARNYLDLYTQLSENSSIMSEP"
     gene            4658..6145
                     /gene="malQ"
                     /locus_tag="P5516_05705"
     CDS             4658..6145
                     /gene="malQ"
                     /locus_tag="P5516_05705"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HRT91967.1"
                     /translation="MTLSRKSGILLHPTSLPGPDGIGDLGPECYRWMDFLHGCGCSLW
                     QILPLGPTGFGDSPYQCFSAFAGNPYLISPTLLLEERLLNRDDFLDRPNFPVEQVDFG
                     AAITWKTTILDRAFSRFKKSRSTKLHQQYQDFCRQEAGWLDDFGLFMAIKEVHGGGSW
                     DGWPVELRLRLPEALSKFSAENTPVVEKHKFRQFIFFSQWQRIFEYAHARGIQIIGDI
                     PIFIAYDSADAWANPELFFLGKDSRPTVVAGVPPDYFSPTGQLWGNPLYRWSAHKRQG
                     YAWWIKRFMSVLRTVDFARLDHFRGFAGFWEIPAGMPTAEIGRWVKGPGKNFLSAINR
                     TLKGLPIIAEDLGLITPDVIDLRDSFHLPGMKILQFAFGSTPEDPFLPHNYPFNCVAY
                     TGTHDNDTTRGWYESAPEIEKDLCRRYLASSGEHIHIDMIRELWKSPAAFAIGPLQDF
                     LGLGSESRMNYPGRPAGNWSWRYLPCQLSPELQRWVKELNFLYSR"
     gene            complement(6155..7129)
                     /locus_tag="P5516_05710"
     CDS             complement(6155..7129)
                     /locus_tag="P5516_05710"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRT91968.1"
                     /translation="MREVIPDKQVDNRAKYLAGLFAGILAVSTASIFIRFAQQEVPSL
                     VIAAGRMTFATLLMLPFGLAELKRNPTAFNKKQIFHLGFAGAALGMHFAAWITSLEYT
                     SIASSVVLVTTAPLWVALVSPALLKERLTKPVVIGLLIALVGGIIVGGSGSCSTVQGK
                     FTCSGIGDLFAGSHSLGNLLAFAGALLSGAYLIAGRKVRNTISLPLYTLIVYGIAAFL
                     LSMMVVLKGDKVTGYATISYVWVLALALIPQAVGHTAFNWALKYLPAAFVSIALLGEP
                     VGTIILAALLLHEAPTFLEMMGGLLVLTGIAVATRKAAPKKIEYEKSQ"
     gene            7264..7875
                     /locus_tag="P5516_05715"
     CDS             7264..7875
                     /locus_tag="P5516_05715"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HRT91969.1"
                     /translation="MPFELPALPYAYDALEPVIDARTVEIHYSKHHATYLKNLNAALE
                     KYPQFYEWPLKKILGSLDQIPEEIRTAVRNNGGGVYNHNVYWFCLSPGGSSAPAGKLK
                     EALEKKFGDFATFKSEFEKAGLTRFGSGYAWLSAKPGGELVLHSTANQDSPLQEGLEP
                     ILVVDVWEHAYYLKYQNRRADYLAGFWDIINWNEVENRFSAIK"
     gene            7906..8310
                     /locus_tag="P5516_05720"
     CDS             7906..8310
                     /locus_tag="P5516_05720"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HRT91970.1"
                     /translation="MTHVITSLCLRDGGCATVCPVECIIPGKPEDKWPWFYIDADTCI
                     DCGACVAECPFEAIFPESEVPAAYKAKGGEKLSMPVGTAGFAEVYDGKNHNGEPVHLE
                     GTRTLAAGETVDLTFCIETNAEFFKSGPGYNA"
     gene            complement(8358..9485)
                     /gene="mltG"
                     /locus_tag="P5516_05725"
     CDS             complement(8358..9485)
                     /gene="mltG"
                     /locus_tag="P5516_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HRT91971.1"
                     /translation="MKPKRHPVFPGCVLLVLLFGIFLSILWVGIPIIARWQFGAPAEQ
                     LTGFSLRNFGLQTLLHRSDLTQASSKAGGEIDFTIESSESINSISHRLAAAGLIKDAG
                     GFRAYLVYKGLDSRLKAGAYKLDPAMTSIEIAERLQAIYSPIVPFFIYPGWRAEEIAA
                     ALPSSGIEVSPEEFLAIVHNPSSLAELTPLSEQSSLNGFLFPGAYQIERKISPDALVL
                     TFVNRFFESVNPGIVSAIHQQGISLYEGITLASIIQRETFEDEERALMASVFYNRLRD
                     GIKLETDPTVQYALGYSAKWGGWWKTPLQTKDLEVDSPYNTYVIQGLPPTPISNPGLS
                     SIMAVAYPEDSPFYFFRAKCDNSGYHDFSITFAEHLSKECQ"
     gene            complement(9475..9909)
                     /gene="ruvX"
                     /locus_tag="P5516_05730"
     CDS             complement(9475..9909)
                     /gene="ruvX"
                     /locus_tag="P5516_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015607.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HRT91972.1"
                     /translation="MGRVLAVDPGEKRLGIAISDPYGVLATPLTVIRHESMTVDCLKI
                     IELCAQNDVSLIVIGQALSASGEESPQVRHTHKLAQTIRSLSPLPVLFWDESESTNLA
                     KKAAIEMGVKKKKRAGHLDEHAAAVILQSYLEAQKGRQANEA"
     gene            complement(9902..11410)
                     /locus_tag="P5516_05735"
     CDS             complement(9902..11410)
                     /locus_tag="P5516_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HRT91973.1"
                     /translation="MKTVILQIEAYDTVASIRDKIKGCRSKRILLVHPRQKRAFPGRL
                     ELDLIARYARENGAAFALVSRNDITREHAESLGISVFGSIPEAQKGEWHEADNPLGQT
                     TPFRGHDELIRMRNNLPKTAATREIRPLVRYLLVGISFLALAALMVFSLPSATLVVSP
                     VPEVQTMTIDIRASTEIAQANLTGLLPAKEESLSVSGQKTIPATGSITVGVEKAHGEV
                     LVRNLFTEAQEIPEGAIFETGGEPNIRFRALDEVNLPADGKGVLVKVEAVLPGAEGNV
                     EPGMVRYLAGGNGLQVAVMNNEPITGGKSINVNSPTEADYQNARLQLLRELEEKALKE
                     IKATVSQDITAIPNSLKFTRVETEERENPIGEPSDMLTINMTATFKMLVYDTVALFSL
                     GEEVMDLSLPEGYHAAKDPLKMIQQGEAVLDQSGEASWQVTFSRQIYRNLQWQQLIPS
                     FRGREKRDVVKVLDEEIPHVKGAEVLTFPEWWPWLPLISDRIYLVERASGNG"
     gene            11516..>12285
                     /locus_tag="P5516_05740"
     CDS             11516..>12285
                     /locus_tag="P5516_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91974.1"
                     /translation="MKKLAIVLGFMGIVLTACNFPLGKLPNDQSQIATRVAATLSAAQ
                     TAAIPTPSSLETIAIVLPTEGPTLTPSPTDTLTPTVTITAPANDPALTLGTPGFFDTF
                     NNGVSFGLATPYEDEGMIIKVESGSMVFKSLALKAGKRWRLTSRNPKNLYLEGTFRTS
                     SCSGTDQYGLVFRAPTYGDGIGYYFGVSCNGQYYFLRWDNSGPTTLVNWTPESKINAG
                     SNQTNRLGVMANDDTFRFYINGSLVKEISDQGITEKGY"
     assembly_gap    12286..12385
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12386..12829
                     /locus_tag="P5516_05745"
     CDS             <12386..12829
                     /locus_tag="P5516_05745"
                     /inference="COORDINATES: protein motif:HMM:NF013122.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRT91975.1"
                     /translation="IDHDGAQLFSKLVKPTRPIPPGAERIHGISNRQVADAQPWPILW
                     PELRSILYGRTIAAYNSSFDARLMEQTHSRYRLPWRERLNFLDVMQLFSDFRAVWDPF
                     RKTYRYFKLAEAGAFFQIDLLNAHRSSADALLTRAVLHSIAGLPY"
     gene            12826..14973
                     /locus_tag="P5516_05750"
     CDS             12826..14973
                     /locus_tag="P5516_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91976.1"
                     /translation="MNPTHLVLVVNASLTEKQSAVITAPARGSLLVTGKAGTGKSTAA
                     VLRMRQMVENGIDGDSILVLVPQRRLAFEYYKAIHSPDFPAGSQPQLLTFNGLAQRML
                     SLFWPLIATDAGFRAAEPPRFLNMENAQYYLAGIVEQLLLQGYFESLTIDPNRLYSQI
                     LDNLNKSAVVGFPPGEIAERLTSAWSGKTSKSNIYQQAQECALKFRQFCLENNFLDFS
                     LQLSVFREKLWPSLLCRQHLNQTFRHLIYDNIEEDFPVAHDFVEEILPNLDSALLIQD
                     EGGGYRSFLGADATSAQRLSGKCSSQIRLTSSLVQTNALANLETALRESILEHRLLKY
                     DSSDARSSYTIHSFRFYPEALDWVTGEIVKLLKDSTTQPGDIAILTPYLSDALRFSFA
                     NRLSSANIPFTTYRPSRSLYAEPAVQAVLTLAQMAHPSWQSKPSPQQARSAFAMVIAA
                     CDFVRADLISRTLFKNRDNLFPLLSFQGLNLEMQSRISFQVGEYYERLRAWLNENLEQ
                     GAHELDHWISRLFGECLSQPGFGFHRDYDAASSINRLIESCREFRKIYQPSAKTAASG
                     AGEEFTRVLQNGLLSSQSNPRSLFETNTDVVFLSPAYSFLMRNQPVKYQFWVDIGSGG
                     WWSRLDQPLTQPYVLNRNWSINQKWTDEHEYLNNQLTLARVVTGLVRRCSAHIYMCSV
                     TYNEHGFEERGQLLLALQTVLRANASIKGSADV"
     gene            14966..17203
                     /locus_tag="P5516_05755"
     CDS             14966..17203
                     /locus_tag="P5516_05755"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HRT91977.1"
                     /translation="MFKPRENQVRILQYQRGKMGISAVPGSGKTHTLSCLASLLILKG
                     ELQNDQEILIVTLVNSAVDNFLNRIGGFIREAQLLENIGYRVRTLHGLAHDIIQERPD
                     LAGVANQFSIIDEIESERMIAQLSAAYIGLHPEIVSHLQNPEADLSSAGRTRNDFSSL
                     VSKLNQDFISQAKDLQAEPETILNRVQRYNCSDPLIKMGIEVYQQYQRGLRYRNALDF
                     SDLIRLAYTALLNDDGYLQRLRQRWPYILEDEAQDSSNLQEKMLRLLVGPQGNWVRVG
                     DPNQAIYETFTTANPKFLKAFLKEQGVQEMTLPHSGRSNLSIITLANSLVQWTRESHP
                     LPSLRSALDYPLIEPTPPGDPQPNPPDHPSAVYISKMKATADEEIKKVVENILNWLPG
                     NQDKTVAVLTPIGWYGEKMAEALHAAGVPMVELLKSSQSTRRVAGLIEKILLALDDPN
                     SSKKFTEAIQSVWTWDTVEGDKLNEIKSLLPLLKKCSRPEDFFFPQPGKTWREAIEPF
                     EVSEERLQEFEDLRLKFTRWQKASFLPIDQLVLTIANDLFSVPSDLALAHKFALLLEF
                     STHLHPAYELKDYAQELAVISGNFRKFSGFSDEELLFDPDAHKGKVFISTYHKAKGLE
                     WDRVYLMSVNNYDFPSAQPYDTYIGEKWFIRDNFNPQAEMLSRLTGLLDGDEALIFAP
                     DGQATAAARVDYAAERLRLLFVGITRARQSLVVTWNSGKNKDKTMALPLEALHAIWRG
                     TDEAA"
     gene            17190..17996
                     /locus_tag="P5516_05760"
     CDS             17190..17996
                     /locus_tag="P5516_05760"
                     /inference="COORDINATES: protein motif:HMM:NF024117.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HRT91978.1"
                     /translation="MKLPDHFSFSQYNLQDYVDCKFRFLLKHIHQLAWPAIESEPLLL
                     QEARMELGQQFHRLVQQYFIGVEPGVLSNSIQTSELADWWQAFLSLKINEQPGTKQAE
                     KMLSVSLNGHRLVAKFDLLLQRSSSSYTIYDWKTSTQAPSIRHLQGRLQSHVYPLVLW
                     LSVTNSVTPPQNLPEIEMVYWFPAFPEVSLRFEYTHSNYEQDLDYLTHLIDEIQEMKE
                     EDFTRTTDVKRCAYCQYRSLCDRGISAGALTPGSEVDSPDSDFYLDFDSL"
     gene            18205..>19163
                     /locus_tag="P5516_05765"
     CDS             18205..>19163
                     /locus_tag="P5516_05765"
                     /inference="COORDINATES: protein motif:HMM:NF013528.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HRT91979.1"
                     /translation="MDRAVSRILHAVDSAQRIGVWGDFDVDGQTSTAILISALRTLGT
                     DPVFHIPVREIETHGIGMAPLQTFLDQGPELILTCDTGVSSFDAVKYAQSRGVDVIIT
                     DHHELPETLPPAYALVNPHRLPEGHRLSSLPGAGTAFKLAEALLTERQMAEKARHLFD
                     LAALGCVADLASLTGETRYLVQSGLNQLRTNPRPALAALLQSSSVEIMRTSEEHISFA
                     LAPRLNAVGRLSDANPIVNFLLSENPVEIAVMVNQIEGLNTQRKYLSDQVFQGALTQL
                     EQERSLLDQPILILHHQEWPAGVVGIVASRLVEIFHRPVILLVA"
     assembly_gap    19164..19263
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <19264..19971
                     /locus_tag="P5516_05770"
     CDS             <19264..19971
                     /locus_tag="P5516_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91980.1"
                     /translation="LAYTFRENNYQGIEQVQFEWVDFRQTRQNGTIEIQQDNHSILHF
                     DYRDSKTPLEDLAQCAATPGLSIWKEGSYSQKVAGVDRLNIQQGETLAVWSIPPDIHV
                     LRHLFLSAHPRQVCWFLVAPAEHQVPGFLHTLGKWVKEGLRLKQQHYTLSDMAHKSAT
                     TVDMVALGLKWLASSGHISILEESPSSYHLVSGGVSDARLKEHLQKELKHSFAELHSF
                     SNYLLHVDLQKLVNELI"
     gene            20003..21058
                     /locus_tag="P5516_05775"
     CDS             20003..21058
                     /locus_tag="P5516_05775"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HRT91981.1"
                     /translation="MKSSRVSRRDFLKLAALGLTSVAFRPDYEHGELADAESLARVAI
                     DADESVSVHSQPDDKSTIKFQRYRDEILNVYYEVISDKSPKYNPLWYRVWGGYIHCAH
                     LQRVKVQINPIPEKLNEGIHLVEITVPYAQSYLQRKPGEWTPIYRLYYGSMHWVSEIV
                     EGFDGLPWYRIKDELIGYENLNYYIPARQARLILPEETSPISPDVNPDKKFIEVSLEK
                     QELTAYEEGRVVLKTKISSGLDYSPPGEIPWKTPTGLFHVQTKMPSKHMGGAELTDDL
                     SAYVLPGVPWTTFFEPENGIAFHGTYWHQNFGMPMSHGCINMKTEEARWLYRWVTPEG
                     DMSKMTTIGFGTQVHVY"
     gene            complement(21052..22260)
                     /gene="hemW"
                     /locus_tag="P5516_05780"
     CDS             complement(21052..22260)
                     /gene="hemW"
                     /locus_tag="P5516_05780"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HRT91982.1"
                     /translation="MEISIYFHIPFCNRRCDYCDFNTYAGLERQIPEYVKALNRELEM
                     VLKNAPTDFKVTTIYFGGGTPSLLNSTDYATLFTTIGNSAVVSPDAEISLEANPDSLS
                     DEKITAYRQVGFNRISIGMQSASRFDLKALGRAHTNQSLLASVDGCRRAGFQHINLDL
                     IFGIPGQTLESWQRTLRLALELGADHLSLYSLVIEEGTRLRRLFDKGLLVEVNDDLAA
                     DMYETAMEELAQAGYSQYEISNWAKNSDARCRHNLQYWRYAPYLGFGAGAHGFWGSTR
                     TENLYTIQEYMQAVNLKSPAAFPAGCACQSTITMNRWEMMQENLMVSLRLTEEGVSLS
                     RFAERYGVELMEVFPHQLEKTLREGLIEYDEENDRLRLTYRGRLFGNRVFSQFVSNKV
                     PKGYEHLLAQ"
     gene            22356..23507
                     /locus_tag="P5516_05785"
     CDS             22356..23507
                     /locus_tag="P5516_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT91983.1"
                     /translation="MTPENVSSTSKTCPTCGSRLSENATRCLVCGRTFTPAATKKTET
                     SPVQNPKLPELKVSLPAAIALIVLILGLGAALIFVILQTTGRVTTPTATPTVTNTPTL
                     TLTPTATLEPTLGPTPTPVPPREYVVKEGDLCSSIALSFGVSIQSIASLNGLPPDCGI
                     LYPNTTLKIPWPTPTPTALPTATLSAAEATQQACGTVPYQVTENDTLSSISANYNISI
                     ESIKTFNGLTSDTVWVGMNLSLPLCERMPTQGPTPTATLLPPYPAASLLLPEDGAVFT
                     NVNESITLQWSSVGTLLEGEAYAVTIEDVTTGGANRLTSYTRETKLTIPSTLRPLDNT
                     FHIYRWSVVAVRQTGTTADGQAEYTPHGTLSQFRVFGWSGAGGAVIPTP"
     gene            complement(23548..24489)
                     /gene="trxB"
                     /locus_tag="P5516_05790"
     CDS             complement(23548..24489)
                     /gene="trxB"
                     /locus_tag="P5516_05790"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HRT91984.1"
                     /translation="MEKMKEERKIESVVILGAGPAGLTAALYAARAELTPLVITGMQL
                     GGQASLTHQIENYPGFPEGIGGADLGDLFKQQAERFGARLAFDVVTEVDLSQLPFRIK
                     TYGQEIFSKTVIVATGADPVHLSVPGEKELTGKGVSYCATCDGYFFKNKHVVVVGGGD
                     SALEEAIFLTKFADKVSLIHRRDTFRAGAILQSRVRENHKIECIMDSVVKEIKGIDKV
                     NAVIIQHVKTAALTELPADGVFIFIGHAPNNGIYQGQLALDEKGYIRVDEKMKTSVDG
                     VFACGEAADANFKQVVVSAGMGAIAAISAGRYLESLE"
     gene            complement(24489..25838)
                     /locus_tag="P5516_05795"
     CDS             complement(24489..25838)
                     /locus_tag="P5516_05795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRT91985.1"
                     /translation="MIEMADADILPYSLEHNFWTWSAQAKVKPIPIKSAKGVYFWDVN
                     GKKYLDLNSMVMCANIGHGDERVIQAMIDQARELPFAGPQFATKPRAMLGKKLAEILP
                     EGLTKYLYTLGGADANENAVKLARAYTGRHKILTRYRSYHGATFGAAALTGDPRRTAW
                     EPAVMPGVVHFLDPFRYRSTFHQRYPDVDEDEFSRDYLGHLEEIIQYEGPQTIAAILI
                     ESVTGTNGIIVPPEGYIQGVRDICNRHHILMIADEVMSGFGRTGEWFAVNHWGVVPDI
                     MTMAKGLTSGYAPLGVVAMKPEIAAAFNEKVYEGGLTYNGHPISLAAAIAVIEVMQQD
                     RLVEKAKISGEILKNMLQNLKARHPSVGDVRSIGLFAAIEVVKNQQTKEPAAPFGGSS
                     PEMSALRQYLLDHGVYLYTHWNTILIIPPLIITQSELEQGLSVIDQSLYEMDKVVNH"
     gene            25932..26318
                     /locus_tag="P5516_05800"
     CDS             25932..26318
                     /locus_tag="P5516_05800"
                     /inference="COORDINATES: protein motif:HMM:NF015690.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HRT91986.1"
                     /translation="MPPGDLTFPREVDLGINLINQGDYFQAHEVLELAWRNEQKKQRQ
                     LYQGLVQVSVLLYHLARGNTKGALKLLKKALLNLTPFVGAATTLDIPRLVSDLNDLRA
                     RIESGQHESGSSLTDRFTLRYRQPED"
     gene            complement(26329..26964)
                     /locus_tag="P5516_05805"
     CDS             complement(26329..26964)
                     /locus_tag="P5516_05805"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF016115.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRT91987.1"
                     /translation="MKINDVTLTIREGMVVWPGEPEVDLHRVKKLEEGANANVTYMGF
                     AVHTGTHVDAPRHFIADGNSVDLLDLNMLIGEVTVVEIPQDVDHISAAVIARSGLAVG
                     TERVLFKTRNSNYWVENRGDFQPGFVGITEDGAQALVELGVKLVGIDYLSIAPYKMSR
                     PTHLVLLKEKMVVIEGLDLSKVKPGKYTLYCLPLKLANADGAPARVILIED"
     gene            complement(26951..>27322)
                     /locus_tag="P5516_05810"
     CDS             complement(26951..>27322)
                     /locus_tag="P5516_05810"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRT91988.1"
                     /translation="YQHEEEENLMYFEKISKDFQLAAEAGADIVQGSQAHSPMGFEFV
                     GDHLIHYGLGNFLFDQMVEWNRREFIDRHILYNGKYINTELLTATLVDWSRPTPMTEE
                     ERTQLLKEIFTVSLKRKNENK"
BASE COUNT         6753 a         7194 c         6306 g         6869 t
ORIGIN      
        1 gttaaaacct gataaaatga tttattcaac ggagtgagat gacacaggct gaaatcatca
       61 cgattggaac cgaactcctg ctgggcgaga tcctggacac caatacacgg tacctggcac
      121 agcagttgcg ccatctcggg attgatttat ttcgcgcgac atcgattggc gataacccgc
      181 tgcgcatcgc tgaaatgata cgagaaagcc tctcgcgggc ggaaatcgtc atcaccaccg
      241 gcggtctcgg cccgaccatt gacgatccta cccgtaatgc cgcggcgatt gcttttaacg
      301 tggagatgga ttttcacgca gacttatgga aagaaatcta ccggaaattc caggttagag
      361 gtgccattcc ctctgacaat aaccagaaac aggcgttcct cccggcgaac gcagtcgcca
      421 ttcacaaccc ggttggaacc gcacctgcgt tttatatcgc ggagcaaaag aaactgctgg
      481 tttgcctgcc gggagtgccc gcagaaatga aaacgatttt tcagcatgaa gtgataccgc
      541 tcctgcgcgg gcacttcgaa cttcaccaac tcatcaagac ccacgtcatc cgcaccagcg
      601 ggattggtga atcggtggtt gacgctcaga ttgccgatct ggaaacatcc agtaacccaa
      661 cagtgggcct ttccgctcat cctggatgtg tggatattcg catcactgcc aaagcaaatt
      721 catcccaaga agcggatagg atgattgatg tcgtcgagca gaaggtcatc tctctgcttc
      781 aaccgcacat ttacggtttt gatcaggata cacttttgga gaaagtcatt gaactcgcag
      841 ccaaccgtgg ggtaacggtt actttattcg ttttttgcgc gatcaacgaa ttgattttat
      901 tttcaaacca caccaacaat aacgtttttg aagttaagaa acttgaccgc ccttataccc
      961 ctcaacaaga cctcaacttg gaaaggcgcg cgttattatt tgccgtaaac atctattgcc
     1021 aggatgatat agcaaccctg gacatgtttt ttaccagccc tcaaaaacat gcggaaaaaa
     1081 agaaactgtt caacaacgct ttaccgcaac tgcaaaaatg gatggaaaac acctgcctgg
     1141 atttcatcag aaggcaactt tctgaggtcg agaatggaga ataacatgaa gacagtcgat
     1201 atcctgcttc gcaatgccga agtgctcacc atggaccatg aatttcgccg ttatgaatgc
     1261 ggagcgctcg ccatcaacgg aaagattatc gaagctgttg gcgacgaaaa agaaattctc
     1321 gcccagtata cagccccgca aatagtagat tgcgcgggta aaattttaat gcccggtctg
     1381 gttaacgcgc acacgcatgt acctatgacc ctgctgcgtg ggcttgcgga tgacctgcgt
     1441 ttggatgttt ggctcatggg gtacatgatg cctgttgagc gagaatttgt ttctcctgac
     1501 ttcgtgcgtt taggtacaca gctcgcttgc gtcgaaatga cccgttcagg aatcacgaca
     1561 ttcgccgata tgtattactt tgaagaagaa gtagccaaag cgaccgccga gtttggcttg
     1621 cgcgcgcttt gcgctcaaac cgtgcttaaa tttccaaccc ctgatgctca gttttacgaa
     1681 gaatctcttg aattgtcacg cgattttatc caacgttgga aaagccatga tctgatcatt
     1741 ccttccgtgg cacctcacgc tccgtacacc tgtacggccg aaatccttaa agccaccgcc
     1801 gccctggctg ccgaatttga tgtccctttg catacgcacc tgtctgaaac ggctgttgaa
     1861 gttgaaaaca tgcgccgaga gaaccgaatg ccggttattc cttatgtaaa aaaacaaggt
     1921 cttttcgaag cgaaggtcct tgctgcccat tgtgtgcatg tggacgacgg tgaaatgcac
     1981 accctgctgc atgtgaacgc gggtgtggcg cacaaccctt cctctaacat gaagttgtct
     2041 tcaggcgttg ccccaatcac ccgcatgctg gagattggcc ttaatgtggg tattggcacc
     2101 gatggtccag cttccaacaa tgacctggac atgtttgaag aaatgcgcct ggcttctttt
     2161 ctgcaaaaag ggtttagtgg agacccaaca accttgcccg ccaggaccac gctgctgatg
     2221 gcaacacgcc ttggagccca ggccatgcac atcggtcatc tgacgggttc tctggagcaa
     2281 ggaaaacgag cagaccttat cctgatcgat attgagccgc tgcataacag ccccagattt
     2341 caacatgacc cgaacggcac ctacgcgcag atcgtctacg cggctaaagc cacagatgtg
     2401 aaagatgtga tggttaacgg caaatggctg atgcgcgatc aacagatcat cggtctggat
     2461 gaagaagatt tattgaagca cagccgcgat tattcgcgca agatcgacca atttctcatc
     2521 caacgagagt cttctgtcct ctccaagctg gttgccatcg gtggtacatc tgaagaagaa
     2581 agctttgaaa ttcagttaaa aattcgcgtc gacgaccgca acaaattgat tgaaaaatta
     2641 ctgcattcag acatggagat tcttcgaaaa cgccattacc gtgaatttga tatttacttc
     2701 ttcttcgcgg atatcaacca gggaatactg cgctaccgtg aagacgagtt ccttaacgaa
     2761 atgggaaaga tcgagcaggt tcgatccagg cttaccttga tcggtccaac cagtgaagat
     2821 aagttcgaac aggaagtttt actgtcacgc agccgctacc tggctccagc cacacaaagt
     2881 ccgcgctttt accgggagta tttcaaaccg cacctggcca aagagattga aaaagaccga
     2941 atccgctatc tggtgaaatt caaggatgta gaattcttta tcaattttga tgaaatcacc
     3001 aaaccaccca ttggtcaatt tgttgaggtg aaaagtcgca cctggagccg tcaggacgct
     3061 gagatgaagt ctcacctcat tactgaactg atcagctttt taggcgccaa cctggacgaa
     3121 gtcacttctg acgattatat tgagatggtc aggtaaacag ataaatgggc ggtgagagtc
     3181 gatcgaaaaa agtgatccga gtgcttttca tcgcttctga ggctgctcca cttgttaaag
     3241 ccggggggct aggagatgtg gctggcgctt taccagccgc gctcaatcgt ctgggtgcag
     3301 ttgtgggggg agcatcagca gaagtgcgcc tggtgatccc tttctacaac tcgatcaaga
     3361 ccagtaatat ttcagcaacc ttcattcgaa aattcaaagt cagcaccttg aatggcgcag
     3421 tggaagccgg attatttgaa acctcccttt caggagtggt cacctatctg gtttccggcg
     3481 caccggtgag cgaatccgct tcgatctatg gaaaggactt taaagaggac gctgaaaagt
     3541 tcgtcttttt ctctctcgcc tgcttaaaaa tcccttcctt gatcggttgg aagtgtgaca
     3601 tactgcacgc caatgattgg catacagcag tggcaataca tcacctagcc acagcgaaga
     3661 agaaaaacgc ctccctgcaa acaacgcgct cggtgctgac aatacacaac ctgcctttta
     3721 tgggaagcgg ggtggatgaa gccctggtga aatacaaaat ttccccatcc aatgatgaca
     3781 acctgccaaa ttgggggaaa acccaacctc ttccaatggg tttggccgcc gctgaccgaa
     3841 tcgtggcggt ctccccgacc tacgcaaaag aaatcctcac gccggagtat ggttgtgatt
     3901 tacaggactt tcttagaaaa agatccggcc atctcactgg tattcttaac ggcattgatg
     3961 tcgaatcctg ggatccggcg acagaccccg cacttaaatt caactttgct gtcagtaatt
     4021 tcaccacacg cagccaaaat aaacgggcct tgctggctga attcggtttt cctgttgaaa
     4081 gcgatgtccc cctgctggct ctcgtcagca gatttgactg gcaaaaaggg atagatatca
     4141 ttatcgccgg tcttcgcgcc atgaaaaatt tcgcctggcg cgccatttta cttggaaccg
     4201 gggagcgcaa ccttgaacag gcctgccgag accttgaaga tgaaatgccg gggaaagttc
     4261 gcgctgccat tctttttgat gtccagctct ccaggcgtat ttatgcgggc gctgatcttt
     4321 tactcatgcc ctcccgttat gaaccctgcg gcctttcaca aatgtatgcc atgcgctatg
     4381 gctgcctgcc aattgccaga gccaccggcg ggctggctga cacggtttct gacccgcttt
     4441 catctacagc accaacaggt tttctcttcc ccaacgctga cgccagttct ttcatcacgg
     4501 ccttaaaacg cgccttgcgc ctgtataaga aacgcgcttt ctggcaggca atgcagttca
     4561 acgcgatgtc gcgtgacttc tcctggtctg tctctgcgcg taactatctt gatctctaca
     4621 cgcaattgtc cgaaaattca tctattatga gtgagccatg accctttcgc gaaaatctgg
     4681 tatcttgctg cacccaacca gtctgccagg ccctgacggt attggtgatc tggggccgga
     4741 gtgttatcgc tggatggatt tcctgcacgg ctgtggttgt tccctatggc agattctccc
     4801 ccttggccct acaggttttg gcgattcacc ttaccagtgc ttcagcgcct tcgccggtaa
     4861 cccttattta atttcaccaa ctttattgct tgaagagcgc cttcttaacc gggatgattt
     4921 tctcgacaga cccaatttcc cagttgagca ggtagatttc ggcgctgcca tcacctggaa
     4981 aaccaccatc cttgaccggg cttttagcag atttaagaaa tcacgttcaa cgaagctgca
     5041 ccagcaatat caagatttct gcaggcaaga agcgggctgg cttgacgact ttggtctttt
     5101 catggcgatc aaggaagttc atggcggagg ctcgtgggat ggctggcccg tagaactccg
     5161 cttgcgcctt cccgaagccc tttcaaaatt ctctgccgaa aatacgcctg ttgtggaaaa
     5221 gcacaagttc aggcaattca ttttcttttc tcaatggcag cgaatttttg aatacgcgca
     5281 cgcgcgcgga attcaaatca tcggtgatat ccccattttt attgcctacg acagcgcgga
     5341 tgcctgggca aaccccgaac ttttctttct gggcaaagat agccgcccga ctgtcgtcgc
     5401 aggtgtcccg cctgattatt tttctcctac cggtcagctg tggggcaacc cactatacag
     5461 atggtctgct cacaaacgcc agggctacgc ctggtggatc aaacgcttta tgtcggtgct
     5521 gagaacagtg gatttcgccc gcctggatca ttttcgcggg ttcgcgggtt tttgggaaat
     5581 acctgccggc atgccaaccg ctgaaattgg gcgttgggta aaaggccctg ggaaaaactt
     5641 cctatcagcc attaacagga ctttaaaagg cttacctatc atcgccgaag acctggggct
     5701 gatcacgcct gacgtgatcg acctgcgtga ctccttccat cttccaggga tgaaaattct
     5761 ccagtttgct tttggtagca cgcctgaaga cccctttttg ccacacaact acccgttcaa
     5821 ctgcgtggct tacaccggaa cccatgacaa cgataccaca agaggttggt atgaaagcgc
     5881 cccagaaatt gaaaaagacc tgtgccgcag atacctggcc agctctggag aacatattca
     5941 tatagacatg atacgcgagc tatggaaatc accagcggca ttcgctatcg gtcctcttca
     6001 ggattttcta gggttgggca gcgaatcccg catgaactat cctggccgcc ccgcaggtaa
     6061 ctggagctgg cgttacctgc cttgccaact gagccccgag ttgcaacgct gggtaaaaga
     6121 attaaacttc ctttacagcc gttaacagct caatctactg gcttttctca tattcaattt
     6181 tcttgggtgc tgctttccgg gttgccacgg caattccagt tagcaccagt agcccaccca
     6241 tcatttccaa aaaggtcggc gcttcgtgca gcagcagggc tgccaggatt atcgttccca
     6301 ccggttctcc aagcagcgca atagaaacaa atgcggcagg cagatacttg agcgcccagt
     6361 tgaaggccgt gtgcccaacc gcctgtggaa taagcgccaa tgccagaacc catacataac
     6421 taatagtcgc ataacctgtc accttgtctc ctttgagcac caccatcatg cttaaaagga
     6481 acgcggcgat accatagaca atcaaagtat aaagaggcag cgaaattgta tttctaacct
     6541 ttctgcccgc aatcaggtaa gcgcctgaaa gcagcgctcc ggcaaaagcc aatagattgc
     6601 caagtgaatg cgaaccagca aaaaggtcac cgatgccgct gcaggtgaat tttccttgaa
     6661 ccgttgaaca gctgccactg ccgcccacaa tgatcccacc gacaagagcg atcaataacc
     6721 caataaccac tggtttggtt aatctctctt ttaggagcgc tggtgaaacc agcgctaccc
     6781 ataacggcgc agttgtgacc aacacaactg aactggcgat tgacgtatat tcaagcgacg
     6841 tgatccaggc ggcaaagtgc attcccagcg cggctccggc aaaaccaagg tggaaaattt
     6901 gctttttatt gaaggctgtc gggtttcgtt ttaattctgc cagtccgaag ggcagcatca
     6961 acagcgtagc gaaggtcatc cttcctgccg cgatgacaag agagggtact tcctgctgtg
     7021 cgaaacgaat aaagatagaa gcagttgaaa ctgccaaaat gccagcgaat aaacccgcca
     7081 ggtatttggc gcggttgtca acctgtttat caggaatcac ttctctcata ccctgtcaag
     7141 aaatacgttt tccagttaca gtgtgttttc ttcacttctg cttgacaatc cttcatctga
     7201 tggctagaat tttaactgaa atgctcgcaa aatacatacg aaaccaaccg atgggaggga
     7261 aaaatgccgt tcgaactgcc cgcacttccg tatgcgtacg acgcgcttga acccgtaatt
     7321 gatgccagaa ccgtagaaat acattacagt aaacaccatg cgacctactt gaaaaacctc
     7381 aacgccgcgc tggagaaata tcctcaattt tatgaatggc cgcttaaaaa gattcttggc
     7441 agccttgacc agattcctga agagattcgt accgccgtca gaaacaacgg tggtggagta
     7501 tataatcata atgtctactg gttctgccta tcacctggcg gctccagcgc gcctgctggc
     7561 aaattaaagg aagcattgga aaaaaagttc ggtgattttg ccactttcaa gtctgagttt
     7621 gaaaaagccg ggctaacccg ttttggcagc gggtatgcct ggttgagcgc caaaccaggt
     7681 ggtgaacttg tcttacattc tacagccaac caggattccc ctcttcaaga agggctcgaa
     7741 cctattttgg ttgtggatgt ttgggaacat gcttattacc ttaagtatca aaatcgccgc
     7801 gcggattatt tggctggttt ttgggatatc attaactgga atgaggtaga aaatcgcttt
     7861 tctgcgataa aataaccata acccattttc aaacggagga atcagatgac tcatgtaatt
     7921 accagtttat gtttgcgtga tggtggttgt gcaacagttt gccctgtgga atgtatcatt
     7981 cctggaaagc cagaagataa atggccgtgg ttctacattg atgcagatac ctgcatcgac
     8041 tgcggcgcct gtgttgcaga gtgtcctttt gaggcgatct tccccgaaag cgaagttccc
     8101 gcagcctata aggcgaaggg cggtgaaaaa ctttctatgc ccgtgggaac cgcaggattc
     8161 gctgaagttt acgatggcaa gaaccataac ggcgagccag ttcatcttga aggaacccgc
     8221 acactcgccg ctggtgaaac agttgacctg accttctgca tcgaaaccaa tgccgagttt
     8281 ttcaaatctg gccctggtta caacgcctga cgttttgtat caactgccaa taaaaaggag
     8341 acccgcgggt ctccttttta ttggcactct tttgagagat gctctgcgaa agtaatagaa
     8401 aaatcatgat accctgaatt gtcgcacttt gccctgaaga aatagaatgg gctgtcttca
     8461 gggtaagcga cagccatgat agaggacagt ccagggtttg aaatcggcgt cggcggcaac
     8521 ccctgtatga cataggtgtt ataaggcgaa tcaacctcca ggtctttcgt ttgcaaaggt
     8581 gttttccacc acccgcccca tttggctgaa tatcccaacg cgtactgcac ggtaggatca
     8641 gtctcgagtt taatcccgtc gcgcaaccga ttataaaaaa cggatgccat cagtgcgcgt
     8701 tcctcatctt caaaggtctc ccgttggatg atggacgcca gtgtgattcc ttcataaaga
     8761 cttattccct gttggtgaat agcgctcaca ataccagggt tcacgctttc aaaaaaccga
     8821 ttgacaaatg ttagtacaag cgcatccggg ctgatctttc gctcaatctg atacgctcca
     8881 ggaaagagaa agccattcaa agaactctgt tcgctcagcg gggttaattc tgcaagagaa
     8941 gatgggttgt gaacaatggc aagaaattcc tccggcgaca cttcaatacc cgaagatggc
     9001 aacgctgctg cgatttcttc agcccgccag ccagggtaaa taaagaaggg gacgattggc
     9061 gaataaatag cctgtaacct ctcggcaatt tcgatactgg tcatcgctgg atccaattta
     9121 taagcgccgg ctttaaggcg tgaatccaac cctttgtaaa ccaggtaagc tctaaacccg
     9181 cctgcgtctt taatcagccc cgctgcggcc aaccggtggc tgatgctatt aatggattct
     9241 gagctttcaa tcgtaaagtc gatctctccg cctgcctttg aggaggcttg agtcagatca
     9301 cttcgatgaa gcagggtttg cagcccgaag ttcctgagcg aaaaaccggt cagttgttct
     9361 gccggcgccc cgaattgcca cctggctatt atcgggatac ccacccataa aatggacaaa
     9421 aatataccga agagcaatac cagtaatacg caacctggaa aaaccggatg tcgcttaggc
     9481 ttcattcgcc tgcctgcctt tctgcgcctc gaggtacgat tgtaaaatta ccgcggcggc
     9541 gtgctcatcc aggtgaccgg cgcgcttttt cttcttcact cccatttcga tagctgcttt
     9601 tttagccagg ttggtgcttt cgctttcatc ccaaaagaga accggtaaag gagacaaaga
     9661 gcggatcgtt tgcgctaatt tgtgtgtgtg cctgacttgc gggctctctt caccgcttgc
     9721 tgaaagcgcc tgcccgatta cgatcaggct gacatcattt tgcgcgcata gctcgatgat
     9781 cttaaggcaa tcaacggtca tgctttcatg tctgatcacg gtcaggggcg ttgccagaac
     9841 gccataaggg tcgctgatgg caattcccaa gcgcttttca ccagggtcta ctgccaaaac
     9901 cctacccatt gccgcttgcc ctttcaacca ggtatattcg atctgaaatc aaaggcagcc
     9961 agggccacca ttcagggaaa gtcagaactt ccgctccttt tacatgaggt atttcttcat
    10021 cgagtacctt tacgacgtct ctcttctctc gccctctaaa agagggaata agttgttgcc
    10081 attgaaggtt tctatagatc tgtcgtgaaa aagtgacttg ccaggaagct tctcccgact
    10141 ggtcgagaac agcttcgcct tgctgaatca ttttaagtgg gtctttcgca gcatggtaac
    10201 cctcaggcag tgaaagatcc attacttctt cacccagtga aaagagtgca acagtgtcgt
    10261 aaacaagcat tttaaaagtc gccgtcatgt ttatcgtgag catgtctgaa ggttcaccaa
    10321 tcgggttttc tcgctcttca gtttccacgc gggtgaattt caggctgttt ggtattgcgg
    10381 tgatgtcttg agaaacggtg gccttgatct ccttcaatgc cttttcttcc agttcacgca
    10441 gcaattgaag tcgcgcgttt tgatagtccg cttcggttgg cgaattaacg ttgattgatt
    10501 tccctcctgt tatcggttca ttgttcatta cagccacctg caagccatta ccacctgcca
    10561 gatatctgac cataccgggt tctacgtttc cctctgcccc aggcaatacc gcttctactt
    10621 tcacgagaac ccccttacca tctgcgggca ggttaacttc atcaagtgcg cgaaatcgta
    10681 tattcggttc tccgccagtt tcaaagatcg cgccttcagg tatttcttgc gcttctgtga
    10741 acaaattgcg caccaaaact tctccgtggg ctttttcaac gcccaccgtg atgctgcctg
    10801 tagcaggaat cgttttctgc cccgatacag acaaactctc ttcttttgca ggtaaaagac
    10861 cggtaaggtt tgcttgggcg atttctgtag atgcacgaat atcaattgtc attgtttgca
    10921 cttctggtac aggggatact accaaagtag cagacggaag cgaaaagacc atcaatgccg
    10981 ccaaagcaag gaaagaaatc ccaaccagca ggtagcgaac aagagggcga atttcgcgtg
    11041 ttgcagcggt ttttggcaga ttattcctca ttctaattag ttcatcatgc ccacggaatg
    11101 gagttgtctg acccagcggg ttgtcggctt cgtgccattc tcccttctgc gcctctggga
    11161 tagaaccaaa aacagaaatg cctaaactct cggcgtgttc acgcgtaata tcgttacggc
    11221 tgacaagcgc aaaggcggcg ccgttttctc gcgcatagcg agcaatcagg tccagttcga
    11281 gcctgcctgg gaatgcccgt ttttgacgag gatgaaccag taagattctt tttgaccggc
    11341 agcctttgat tttatcgcgg atcgaagcga cagtatcgta ggcttcaatt tgcaaaatca
    11401 ccgttttcat agggtttaca gaattatact gtagggctgg ttgaaattaa tccgctcctc
    11461 actgttctat tcacattgac agagtttata atagaaaaag aaaagtgagg taaagatgaa
    11521 aaaacttgcc atcgtacttg gtttcatggg tatcgtgctt accgcctgta atttccctct
    11581 gggtaaactt cccaacgacc aaagtcagat cgccacgaga gtcgcagcga cattgtccgc
    11641 ggcgcaaacc gccgctattc ccaccccttc ctcgctcgag accatcgcaa tcgtgctacc
    11701 gacagaaggg cctaccctga ccccatcgcc caccgatacg ttaacgccca cagtcactat
    11761 caccgccccc gcaaatgatc cagcccttac ccttggcact cctggctttt ttgacacctt
    11821 taataacgga gtttcttttg gcctggcgac cccctacgag gacgagggca tgataataaa
    11881 agtagaaagc ggctccatgg tctttaaaag ccttgccctc aaagcgggaa aaaggtggcg
    11941 gttgacgtcc agaaatccca aaaacctgta cctggaaggc accttccgca cttcctcctg
    12001 tagtggaacc gaccaatacg ggctggtgtt tcgtgcgccc acttacggtg atggtatcgg
    12061 ttattatttt ggcgtgtctt gcaacgggca gtattatttc ttgcgctggg ataattcagg
    12121 ccccactact cttgtgaact ggacaccgga atccaagatc aacgcgggca gcaaccaaac
    12181 gaaccgttta ggagtgatgg cgaatgatga tacattccgc ttttacatca atggttcgct
    12241 ggtgaaagaa atttctgatc agggaatcac tgaaaaaggc tatatnnnnn nnnnnnnnnn
    12301 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12361 nnnnnnnnnn nnnnnnnnnn nnnnnatcga ccatgacggc gctcaacttt tctcaaaact
    12421 ggtgaaacct accaggccca ttccgccagg agcggaaaga atacatggga tttccaatcg
    12481 tcaagtggct gacgcccaac cctggcctat cctttggccg gagctcaggt cgattctata
    12541 tggaagaacc atcgcggctt acaattcgtc ctttgatgcc aggctaatgg aacaaactca
    12601 ctcgcgttac cgcctgccct ggcgcgagag gctgaatttc ctggatgtga tgcaattgtt
    12661 ctctgatttc cgcgctgtgt gggacccttt ccgaaaaacc taccgttact ttaaacttgc
    12721 cgaagcaggc gcattttttc agattgactt actcaacgcc caccgctctt ccgctgacgc
    12781 cctgctcacc agggctgtac ttcactcgat tgccgggttg ccgtattgaa tccaacacat
    12841 ctcgtacttg ttgtaaatgc ttcactcaca gaaaaacaat ccgcggtgat tactgcccca
    12901 gcacgtggca gcctccttgt gacggggaaa gccggtacag gcaaatctac cgcggcagtg
    12961 ctgcgcatgc gccagatggt ggaaaacggt atcgatggcg acagcatcct tgtccttgtg
    13021 ccgcagcgcc gtctggcatt tgagtattac aaggcaatcc attcaccaga tttcccagcc
    13081 ggcagtcagc cacagttact tactttcaat gggctggctc aacgcatgct ttccttattc
    13141 tggccgctta tcgccacaga tgctggtttt cgcgcggcag aacctccacg gtttctcaac
    13201 atggaaaatg cccagtatta cctggcaggt attgttgagc agttgttgct gcagggatac
    13261 tttgaaagcc tgacaattga cccgaaccga ctttacagcc agatacttga caacctgaac
    13321 aaatctgccg tagtaggatt ccctcctggt gaaatcgctg agcggcttac atctgcctgg
    13381 tctggtaaaa cctccaaatc caatatctat caacaggctc aggaatgtgc cttaaagttc
    13441 cggcagtttt gcctggagaa caacttcctc gacttttcac tgcaattatc cgttttccgc
    13501 gaaaaactgt ggccctcgct gctctgcagg caacacctga accagacctt ccgtcacctt
    13561 atctacgata acatagaaga agatttccct gttgcccacg attttgtaga ggagatcctt
    13621 cccaaccttg attccgcgct gttgatacaa gatgaaggcg ggggttatcg ctcatttctt
    13681 ggcgcggatg ccaccagcgc ccagcgccta tcgggcaaat gttccagtca gatccgcctg
    13741 acctcatcgc tggtgcaaac caatgcgttg gcaaaccttg aaacggcgtt acgtgaaagc
    13801 atccttgaac atcgattgtt gaagtacgac tcctcggacg cgcgtagcag ctataccatc
    13861 cattcctttc gtttttaccc cgaggcgctc gattgggtca caggtgaaat tgttaaactg
    13921 ctaaaagaca gtaccacaca gcctggcgac atcgcgatcc tcactccgta cctcagtgat
    13981 gccttgcgtt tttcattcgc caatcgtttg tcgagcgcga acatcccttt cacgacctac
    14041 cgtccttcgc gcagtttata tgcagagccc gcggtgcagg cggtccttac cctcgcccaa
    14101 atggcgcatc cttcatggca gagcaaaccc tccccacagc aggccagatc agcgtttgcc
    14161 atggtcattg ccgcgtgtga ttttgtacgg gcagatctca tttcccgtac gctttttaaa
    14221 aacagggaca acctctttcc tcttctttct ttccaggggc tcaacctcga gatgcaatct
    14281 cgaatttcct tccaggtggg cgagtattac gaacgcctgc gcgcttggct gaacgagaat
    14341 ctggaacagg gcgcacatga gctcgaccat tggatcagcc ggctttttgg cgaatgtctt
    14401 tcccagcccg gcttcggctt tcatcgagat tatgatgccg cgtcgtcgat taaccgtctg
    14461 atcgaatcct gtcgggaatt tcgtaaaatt tatcagccga gcgcgaagac tgcggcttca
    14521 ggcgctggtg aagaattcac ccgtgtgttg cagaatggct tgctctcctc tcagtccaac
    14581 ccgcgttctc tttttgaaac caatacggac gtagtttttc tttctcccgc ttactctttt
    14641 ttaatgcgca atcagccggt taaatatcag ttctgggtcg atattggttc aggcggttgg
    14701 tggtccaggt tagaccagcc cttaacccaa ccctatgtgc tcaatcgtaa ctggtctatc
    14761 aatcaaaaat ggactgatga gcacgagtat ctcaataatc aattgacgct tgctcgggtt
    14821 gtcaccggtt tggtcaggcg ctgtagtgct cacatctata tgtgcagcgt cacctataat
    14881 gagcatggct tcgaagaacg cggtcaattg ctgttagcac tacaaactgt tctgcgtgca
    14941 aacgcgagta taaaaggtag tgcagatgtt taaaccacgc gagaaccagg ttcgtatttt
    15001 gcagtaccaa cgcggaaaaa tggggatttc tgctgttccc ggcagcggca agacgcacac
    15061 cctttcctgc ctggcttccc tgctgattct caagggggag ctgcaaaacg atcaggaaat
    15121 cctcatcgtg acgctggtca attccgcagt tgataatttc ctcaaccgca ttggtggttt
    15181 tattcgagaa gcgcagttat tggaaaacat cggttaccgt gtgaggacac tgcacggttt
    15241 ggctcacgat atcatccagg aaagacctga ccttgctggt gtggccaacc agttttcaat
    15301 catcgatgag attgaatcag aacgtatgat tgcgcagtta tcagctgcgt acattggttt
    15361 acatcctgaa atcgtttctc atctgcaaaa cccagaggcc gacctttctt cagccgggcg
    15421 tacgcggaac gatttttcct cactggtttc aaaactgaac caggatttca tcagccaggc
    15481 gaaagacctg caggccgaac ctgagaccat tctcaaccgc gtgcaacgct acaattgttc
    15541 cgacccgctg attaagatgg ggatcgaggt gtaccaacaa tatcaacgcg ggttgcgtta
    15601 ccgcaacgca ctcgacttct cagacctcat ccgcctggcg tatactgccc tactcaatga
    15661 cgatggatat cttcagcgcc tgcgtcaacg ctggccatat attctggaag atgaagcgca
    15721 ggattcttct aacttgcagg aaaaaatgtt gcgcctgctc gttggtcctc aggggaactg
    15781 ggtgcgtgtg ggcgacccca accaggctat ttacgaaacg tttactaccg ccaatccgaa
    15841 atttcttaaa gcctttctca aagagcaagg cgtgcaagaa atgaccctgc ctcactcggg
    15901 cagatcaaac ctcagcatta ttacgctggc caactcactc gtccagtgga ccagggaatc
    15961 tcacccgctt ccctcgctgc gcagcgcgct cgattacccg ctcatcgaac ccactcctcc
    16021 cggagaccct caaccaaatc ctcccgatca tccaagcgcg gtttacatca gtaaaatgaa
    16081 agccactgcg gatgaagaaa ttaaaaaagt agtcgaaaac atcctgaatt ggcttcccgg
    16141 caaccaggat aagacagtgg ccgtgctcac acctatcggc tggtatggtg aaaaaatggc
    16201 agaagcgctg cacgctgccg gtgtgccgat ggtggaattg ctgaaaagca gccagtcaac
    16261 acgccgtgtc gccgggttga ttgaaaaaat tctgcttgcg ctggatgacc ccaacagcag
    16321 caagaaattc actgaggcaa tccagtctgt atggacctgg gatactgtag agggcgataa
    16381 acttaatgaa atcaagtcgc tactaccatt gctcaagaaa tgctcccgac cggaggattt
    16441 cttcttccct caacctggta aaacctggag agaagccatt gagccttttg aggtttcgga
    16501 agagcggctg caagaatttg aggatttgcg cttgaaattc acccgttggc aaaaggcatc
    16561 tttccttcca attgaccaac tggtgcttac tatcgctaac gatcttttct ctgtgccttc
    16621 tgatttggcg ctggcgcaca agtttgcttt gctattggag ttcagcactc accttcaccc
    16681 agcctacgaa ctaaaagatt atgcccaaga gctcgcggtg atttctggta attttcgaaa
    16741 gttcagcggt ttcagtgatg aagaactgct ttttgacccc gacgctcaca agggcaaagt
    16801 gtttatttca acttatcaca aagctaaagg gctcgagtgg gaccgggttt acctgatgtc
    16861 ggtcaataac tatgattttc cttctgctca accatacgat acttatattg gtgaaaaatg
    16921 gttcattcgc gacaatttca acccacaggc ggagatgttg tctcgcctta caggactact
    16981 ggatggggat gaagccctga tttttgcccc cgatggtcaa gccactgcag ccgcccgagt
    17041 ggattacgct gctgaacggc tgcgcctgct ctttgttggc atcactcgag cccgtcaatc
    17101 gctggtggtc acctggaact ccggcaaaaa taaagacaag acgatggcgc ttcccctaga
    17161 agcccttcac gccatctgga gaggcactga tgaagctgcc tgaccatttt tcattttctc
    17221 aatataacct gcaggattat gtggactgca aattccgctt cttgttaaag cacatccacc
    17281 aattggcatg gccggcgatt gaaagcgaac ctttattatt gcaggaagcg cgtatggagc
    17341 ttgggcagca atttcaccgc ttagtacaac agtacttcat cggggttgag ccaggggtcc
    17401 tgtccaattc cattcaaacc tcagaactgg cagattggtg gcaggctttt ctttctctca
    17461 aaatcaatga acagccaggc acgaagcagg ctgagaaaat gttatccgtt tccttgaatg
    17521 ggcatcgcct ggtagcaaaa ttcgacctgc tgctgcagag atcttcatcg tcttatacga
    17581 tttacgactg gaaaaccagc actcaagcgc cctccatacg acatctccaa ggacggttgc
    17641 agagccatgt ttaccctctg gtgctgtggc tttctgttac aaacagcgtg accccacccc
    17701 agaatttacc cgagatcgaa atggtctact ggttcccagc cttcccggag gtatcgctcc
    17761 ggtttgagta cacgcattct aactatgaac aggatttgga ttacctgaca catttaatcg
    17821 acgagataca agaaatgaag gaggaagact tcacccgcac aacggacgta aagcgttgcg
    17881 cgtattgcca atatcgttct ttgtgtgacc gggggatttc cgccggcgcg ctcacacccg
    17941 gttcagaggt agattcacct gattcagatt tctacctgga tttcgactcc ctataaaacc
    18001 agtatgtacc gaccactcca cattgaccgc agttgggtat ttcaagagcc agtaccggtt
    18061 cccagggcct tgacttcgct gggggattac cctgatattg tttttcaaac gttgacacgc
    18121 aggggtttta atgacattca tctcgcccgg caatttatag accccgccgc ctacgagcca
    18181 agtccgcctg ttgaattacc ggatatggat agagccgtct cgcgtatctt gcacgctgtc
    18241 gattctgctc aaagaattgg tgtttgggga gacttcgatg tggacgggca aacttcaacc
    18301 gccattctga tttcagcatt gagaacgctt ggcactgatc ctgtcttcca cattccggtg
    18361 cgagaaattg aaacacacgg aattggaatg gctccactgc aaaccttctt agaccaaggc
    18421 ccagaattga tcctcacctg cgacacaggt gtttcatctt ttgacgccgt caaatacgcc
    18481 caatcacgcg gcgtcgatgt catcatcacc gaccatcacg aactgcctga aacgctccca
    18541 ccggcttacg ccttggtgaa tccgcaccgc ctcccagagg ggcaccgatt aagcagtttg
    18601 ccaggcgccg ggaccgcttt caaactggct gaagccttgt tgaccgaacg gcaaatggca
    18661 gaaaaagccc gtcatttgtt cgaccttgcc gcgcttggtt gtgtggcaga cctggcttcc
    18721 ctcactggtg aaacccgtta cctggtgcaa tcgggtttga atcaattgcg taccaacccg
    18781 cgcccagcgc tggcagcgct gctccagtct tcttctgtcg aaataatgag aacctcagaa
    18841 gaacacatct cctttgctct ggctccacgc ctgaacgcag taggaaggct cagcgatgcc
    18901 aatcccatcg ttaatttctt gctttcagag aacccggtag aaatcgcggt gatggttaac
    18961 caaatcgagg gactgaatac acagcgaaaa tatctaagcg accaggtttt ccagggtgct
    19021 ttgacccaat tggaacaaga gcgctctttg ctcgatcaac caatactcat tctccaccac
    19081 caggaatggc ctgccggggt tgtgggtatt gttgccagcc ggttggtgga aattttccac
    19141 cgccctgtta tcctgctggt tgcnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19201 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    19261 nnnctcgcgt atacctttcg cgagaataat tatcaaggta ttgaacaggt gcagttcgag
    19321 tgggtcgatt tcaggcagac gcgccagaac ggcacgattg aaattcaaca agacaaccac
    19381 tcaatcctcc atttcgatta ccgagattca aaaacgccgc tcgaagacct ggctcaatgc
    19441 gctgcaaccc caggtctctc aatttggaaa gaaggctctt acagccagaa agtggctggg
    19501 gttgatcggc tgaacatcca acagggcgaa acgctggcag tttggtccat accgcctgat
    19561 attcatgtgt tacgccatct attcctctct gctcaccctc gccaggtatg ttggttcctg
    19621 gtcgcccctg ctgagcatca ggttcctggc tttctgcaca ccctgggcaa atgggtaaaa
    19681 gaggggttgc gcttgaagca gcagcattat accctttccg atatggctca caaaagcgcc
    19741 actaccgtgg atatggttgc cctcggtcta aaatggttgg ccagctccgg acatatttct
    19801 atcctcgaag aatcgccctc aagttaccac cttgtttcag gtggcgtgtc agatgccagg
    19861 ctgaaggagc atctacagaa agagttgaag cattctttcg ccgaattgca ttcattttcc
    19921 aattacctgt tgcacgttga cctgcaaaaa cttgtgaacg agttgattta acgctgggtt
    19981 ttcattgtta taatgcctat ttatgaagag tagtagagtc tctcgcagag attttctcaa
    20041 actggccgcg ttggggttaa ccagcgtggc attcagaccc gattatgagc atggcgagtt
    20101 ggcagatgct gaaagccttg ccagagttgc catagacgcg gatgagtctg tcagtgtcca
    20161 cagccagccc gatgacaaaa gcacaatcaa attccaacgt tatcgcgatg aaatattgaa
    20221 cgtttactac gaggttatct ccgataaaag cccaaaatac aacccgttat ggtaccgggt
    20281 gtggggtggt tacattcact gcgcacacct gcagcgtgta aaagtccaaa tcaatcccat
    20341 tcctgaaaag ctgaatgaag gcattcatct cgtcgaaata accgtgccct acgcgcaaag
    20401 ttatttgcag cgaaaacccg gggaatggac gcctatctac cgcttgtatt atggcagcat
    20461 gcactgggtt tcagaaattg tagaaggatt cgacgggctg ccttggtatc ggataaaaga
    20521 tgaactcatt ggctatgaga atctgaatta ttatattccc gcccggcagg cgcgattgat
    20581 cttaccagaa gaaacttcac ctatttctcc tgatgtaaac ccggataaaa aattcatcga
    20641 ggtatcactg gagaaacagg aattgaccgc ttatgaagaa ggacgggttg tacttaaaac
    20701 gaagatttca tcagggcttg attacagccc tcctggtgaa atcccctgga agacgcccac
    20761 tggtctcttt cacgtgcaga ccaaaatgcc ctccaaacac atgggcggcg ctgaacttac
    20821 tgatgacctg tcagcctatg tcctgcccgg ggtcccttgg acaacgtttt tcgaaccaga
    20881 aaacggaatc gcttttcacg gcacttactg gcaccagaac ttcggtatgc cgatgagtca
    20941 tggctgtatc aacatgaaaa ccgaagaagc gcggtggttg taccgctggg tcacacctga
    21001 aggcgatatg agtaaaatga ccaccatcgg cttcggcacc caggtgcatg tctactgagc
    21061 caacaggtgt tcgtatcctt tcggaacctt attcgaaaca aattgagaga aaacgcgatt
    21121 gccgaatagc ctccctctgt aggttaacct caagcggtca ttttcttcgt cgtattcgat
    21181 taacccctcg cgtaaggttt tttccagttg atggggaaaa acttccatta attccacacc
    21241 ataacgctca gcaaacctgg ataaagatac cccttcttcc gtaaggcgta atgaaaccat
    21301 caagttttct tgcatcattt cccaacggtt catggtgatc gtggactggc aagcacatcc
    21361 cgcagggaaa gctgccgggg atttcaaatt cacagcctgc atatattcct gaatcgtgta
    21421 caaattctcg gtcctggtag aaccccagaa gccatgtgct ccagcgccaa acccgagata
    21481 aggagcgtaa cgccagtact gcaggttatg ccggcagcgt gcatcgctat ttttggccca
    21541 gttggaaatt tcatactggc tgtaaccagc ctgtgcgagc tcctccatgg ctgtttcata
    21601 catatccgcg gcgaggtcgt cattcacttc aaccagcaat cctttatcaa ataaacgcct
    21661 tagccttgta ccctcctcaa tgaccaatga atacaacgac agatggtcag cgcctaattc
    21721 aagcgccaac cgcagggttc gctgccagct ctcaagcgtt tgccccggaa tgccgaagat
    21781 cagatccagg ttaatatgtt gaaagcctgc tcgtctgcat ccatccacac tcgcgagcag
    21841 agattgattc gtatgcgccc tgccaagcgc cttcaaatcg aaccttgacg ccgattgcat
    21901 gccgatactg atacggttaa aacccacctg tcgataagcg gtgatttttt catccgataa
    21961 tgaatccggg ttggcttcga gagaaatttc cgcatctggc gaaaccactg cgctattccc
    22021 tattgtggta aataaggtcg cgtaatccgt actgttcagt agtgacggcg taccgccgcc
    22081 aaagtaaatg gtggtcacct taaaatccgt tggggcattt ttaagaacca tttccagctc
    22141 gcgattgagc gccttgacat attcagggat ttgtctttcc aggccagcat atgtgttaaa
    22201 atcacaataa tcgcatcggc gattacaaaa agggatgtga aaataaatac tgatttccat
    22261 aattttcagg ttagaataac acaatcttga attgatgcaa aggaagcggc ccgtgatcgg
    22321 tttttccgat tacgctccat gaagagataa ttcatatgac accagaaaat gtatcttcca
    22381 cttcaaaaac ctgtccaacc tgtggctcaa ggttgagtga aaatgctacc cgctgcctgg
    22441 tttgcgggcg caccttcacc cctgccgcaa cgaagaagac cgaaaccagc ccggtgcaaa
    22501 accctaaact acccgagtta aaggtcagcc ttccggcagc catcgccctc attgtattga
    22561 ttcttgggct tggagcggcg ctaattttcg tgatccttca gacaaccggt cgcgttacca
    22621 cacctaccgc cacccccacc gtgacgaata cccctaccct cacgcttacc cccacagcca
    22681 ccctggagcc aaccctaggg ccaaccccga caccggttcc tccgcgtgag tatgtggtaa
    22741 aagagggtga cctgtgcagt tcgatcgctt tgtcattcgg cgtatctatt caaagtatcg
    22801 caagcctgaa tggtttaccg cctgactgcg gcatccttta ccccaacacg acattgaaaa
    22861 tcccctggcc aacaccaacc cctaccgcgt taccgaccgc cactctcagc gctgctgaag
    22921 ccactcagca agcctgcggc accgttcctt accaggtaac ggaaaatgac accctcagct
    22981 ctatttcggc taattacaac atcagcattg aatcgatcaa gacctttaat ggtctcacct
    23041 ctgacaccgt ttgggttggt atgaacctga gcctaccgct ttgcgagcgc atgcccactc
    23101 agggccccac acccaccgcc accctgctgc ccccttaccc agcggccagc ctgttgctgc
    23161 ctgaagatgg cgcggttttt accaacgtca atgagtccat caccctgcaa tggtcttccg
    23221 ttggcacttt gttggaagga gaagcctacg ccgttacaat tgaagatgtg actacaggtg
    23281 gcgcgaatag gctgacttct tatacgcggg aaaccaaact gaccatcccc agcacgctgc
    23341 gccccttgga caacacgttc catatttacc gctggtcagt agtggcagta cggcaaaccg
    23401 gcaccactgc tgacggtcag gcagaataca cccctcatgg taccttgagc caattccggg
    23461 tgttcggttg gtctggagca ggtggcgcgg tcatacctac accataaaaa gagctcccct
    23521 ctgcttctct tcagagggga gtatttttta ttccaggctt tcaagatacc tgccggcaga
    23581 aattgcagcg atggctccca ttcctgctga aacaaccacc tgtttgaaat ttgcatctgc
    23641 tgcctcgccg caggcgaaca ctccatccac actggttttc attttttcat caacccggat
    23701 atatcctttt tcatccagcg ccaattgacc ttgataaatg ccattattgg gcgcgtgacc
    23761 aataaatata aaaacaccat cggctggtaa ctctgttagc gcagcggttt tcacatgctg
    23821 aatgatcacc gcgttgactt tgtcaatccc tttaatttcc ttaacgaccg aatccataat
    23881 gcattcgatt ttgtgatttt cacgtacgcg tgattgcaaa atcgcacccg cgcgaaaagt
    23941 gtccctgcga tgaatcaaac tcactttatc ggcaaatttg gtgaggaaga tcgcctcttc
    24001 cagcgcgctg tcccctcctc ctaccactac tacatgctta tttttaaaga aatatccatc
    24061 acaggtcgcg caataagaaa cgcccttccc ggtcagttct ttttcgccag gtacgctcaa
    24121 atgtacgggg tcagcgcctg tcgcgacaat caccgttttt gaaaaaatct cttgaccgta
    24181 cgtcttaatg cggaaaggga gctggcttaa atcaacctca gtcaccacat caaacgccaa
    24241 cctggcgcca aaccgttcgg cttgttgttt aaaaaggtca cccaaatctg ctccgccaat
    24301 tccttccggg aacccgggat aattctctat ttggtgtgtt aaagaagcct gccctcccaa
    24361 ttgcataccg gtaataacca gcggcgtcag ttcggcccgg gctgcataga gcgccgctgt
    24421 taaaccagcc ggccctgccc ccaggataac cacagattca attttccgct cttctttcat
    24481 cttttccatc agtgattgac caccttatcc atttcgtata aagactgatc gataacgctc
    24541 agcccttgtt caagttccga ctgggtgata atcaaaggag gaatgatcag gatggtgttc
    24601 caatgcgtgt aaaggtaaac gccatgatcc agcaaatact ggcgtaacgc gctcatttca
    24661 gggctggaac ctccaaacgg cgcagccggt tctttggttt gctggttttt taccacttca
    24721 attgccgcaa acaagcctat tgaccttacg tcaccgacag atgggtgcct ggctttcaaa
    24781 ttttgaagca tatttttcaa tatctcgcca cttattttgg ctttttcaac cagtcgatct
    24841 tgctgcatca cctcgattac tgcaatggca gccgccagtg agatagggtg tccattatac
    24901 gttagtccgc cttcatacac tttctcgtta aacgccgccg cgatttcagg tttcatagcc
    24961 accacgccca acggggcata acccgaggtc aaccccttgg ccatggtcat gatatccggc
    25021 acaacgcccc aatggttcac agcaaaccat tcacccgtcc gtccaaatcc actcatcacc
    25081 tcgtcagcga tcataaggat gtgatggcga ttgcaaatat cacgaacccc ctggatgtaa
    25141 ccttctggcg gaacaataat accgttcgta cctgtcacgc tttcgatcaa aatggcagcg
    25201 atggtctgcg gtccctcgta ttggatgatt tcttccaggt gccccaggta atcacgcgag
    25261 aactcgtctt catcgacatc tgggtagcgt tgatggaaag tggaacgata tcgaaacggg
    25321 tcaagaaaat gcaccactcc cggcatcaca gccggctccc aggccgtccg tcgggggtcg
    25381 ccggtcaaag cagccgcccc aaatgttgcg ccatggtatg agcgatagcg ggtgagtatt
    25441 ttatgccttc cggtgtaggc gcgtgccagt ttcaccgcgt tttcattggc gtcggctccg
    25501 cccaatgtgt agaggtattt ggtcaacccc tctggcaata tctcagccag tttttttccc
    25561 aacatcgccc gcggtttggt ggcaaattgc ggacccgcga aaggcagttc tcttgcctgg
    25621 tcaatcatcg cctggatgac ccgttcgtcg ccatggccaa tgttggcgca catcaccatt
    25681 gagttcagat caaggtattt tttgccattc acgtcccaaa aataaacccc tttagcggat
    25741 tttattggga tgggcttgac ttttgcttgc gcggaccagg tccagaagtt atgctcaagc
    25801 gaatacggca gtatatctgc atcagccatt tctatcattt ttccctctgt tgccaaaaga
    25861 gatgagacaa tccactatta tcctatcata aatcgtgttt cctggaatct atttcgtggt
    25921 agagtaatcc tatgccacca ggtgacctga cttttccaag agaagttgat ttagggatca
    25981 acttaatcaa ccagggagat tatttccagg cgcatgaggt tcttgagctt gcctggagga
    26041 acgagcaaaa aaaacaacga cagttgtacc aggggctggt tcaggtcagc gtgttgttat
    26101 atcatcttgc aagaggaaat acgaaaggcg cattgaagct attaaaaaaa gccctgctca
    26161 atctcacgcc ctttgtgggc gcagctacta cactggatat tccaagactg gtgtctgacc
    26221 tcaatgattt gcgggcgcga atagaatccg ggcagcacga atctggttca tctttgactg
    26281 atagattcac gctgcgctat cgtcaacctg aggattgagc cgctcagctc aatcttcgat
    26341 cagaatcacc cgtgccggtg caccgtcagc attcgccagt ttcagcggca ggcaataaag
    26401 cgtgtacttc cctggtttta cctttgaaag gtccaaccct tcgataacca ccattttttc
    26461 tttcaacaag actaggtggg ttggacggct cattttgtat ggcgcgatgg agagatagtc
    26521 aatcccaacc agctttacac ccaattccac cagcgcctgc gctccatctt cagtgattcc
    26581 cacaaagcca ggctgaaaat ctcccctgtt ttcaacccag taattggagt tacgagtttt
    26641 gaataacact ctctcagtac cgaccgctaa acctgatcgg gcaattacag cagccgaaat
    26701 atgatcaacg tcttgaggga tttcaaccac agttacctcg ccgatcaaca tgttcagatc
    26761 caacaggtca actgaattgc cgtcagcgat aaaatggcgg ggagcgtcta catgggtgcc
    26821 agtatgcacg gcgaaaccca tataagtaac gttggcgttg gcgccttctt cgagtttctt
    26881 gacccggtgt aaatcaactt cgggttctcc tggccaaacg accatgcctt ctcgtattgt
    26941 gagtgttaca tcatttattt tcatttttcc tcttcaaact tacggtgaat atttccttca
    27001 ggagttgggt tctctcctct tccgtcatcg gtgtcggacg cgaccagtcc accagagtgg
    27061 cggtcaataa ctcagtgttg atgtattttc cattgtatag gatatggcgg tcaatgaatt
    27121 cgcgccgatt ccattctacc atctggtcaa acaggaaatt tcctaaaccg taatggatta
    27181 ggtgatctcc tacaaattca aaacccatcg ggctatgcgc ctgcgagccc tgtacgatat
    27241 cagcgcctgc ttcggctgcg agttgaaaat ccttgctgat tttttcaaaa tacatcaagt
    27301 tttcttcttc ttcgtgctgg ta
//
LOCUS       DAOTLW010000028        25971 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_2222_length_25971_cov_10.699838, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000028 DAOTLW010000000
VERSION     DAOTLW010000028.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 25971)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25971)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25971
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2222_length_25971_cov_10.699838"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            23..874
                     /gene="cbiQ"
                     /locus_tag="P5516_05815"
     CDS             23..874
                     /gene="cbiQ"
                     /locus_tag="P5516_05815"
                     /inference="COORDINATES: protein motif:HMM:NF014420.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HRT91989.1"
                     /translation="MASPALKSAHPLKTTPKGRRLRELYTPNHSAVHRIDARVKVIFT
                     LAFIVFLNLTPSGAWPAYILFFTWMFSLSLSSRLGIAFVLKRSLLALPFVLAALPLVF
                     TGPAPLGQIPVLNTPSLTYSVVGFYRFASIAIKAWVSVMAAVLLAATTRFPDLLTAFS
                     QLKVPTLFITIIGLMWRYLFVISDEVTRMLRARTSRSATAPRSNRSGGSLFWRARVTG
                     GMAGNLFLRSIERSDRVYAAMLSRGYTGQPAAQKTTRLQKKDLLVLAAGVILLVILWL
                     LGVITGG"
     gene            878..1582
                     /locus_tag="P5516_05820"
     CDS             878..1582
                     /locus_tag="P5516_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT91990.1"
                     /translation="MEPVIEIRNLSFSYPDGKQALAGINLLVQPGEKVALVGANGAGK
                     STLLYHLNGIFTGSGMIRVNQLELNKNNLPRVRAMVGLVFQNPDDQLFSPTVYEDVAY
                     GPTYQGFDPHIVREKVALALKAVHMEDYTLRNPYHLSSGEKKRIAIATVLSMQPQILA
                     FDEPTAGLDPRARRELIDLLLELQQTMIIATHDMNMVEQLTPRTVLLNHGKIAADGPS
                     LDILRNDRLLMTNGLI"
     gene            1658..2437
                     /gene="larB"
                     /locus_tag="P5516_05825"
     CDS             1658..2437
                     /gene="larB"
                     /locus_tag="P5516_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HRT91991.1"
                     /translation="MDQETLRKLLENLIKGELTVPQVIDQLKTLPYEGLNDYAKLDHH
                     RPLRTGLPEVIFAQGKTPQQVLDIFTRLQKSHHQVLATRVSPGLYAEIQDHLSGVTYD
                     STARLLFTSPDTRQVKRPGIVVLTAGTSDIPVAEEAAITAELMGNKVERIYDVGVAGL
                     HRLISHLPVLQKARVVITVAGMEGALPSVVAGLVSAPLIAVPTSVGYGASFQGLAALL
                     AMLNSCASGVAVVNIDNGFGAGCMAAKINRLSSNESGNEPS"
     gene            2424..3278
                     /gene="larE"
                     /locus_tag="P5516_05830"
     CDS             2424..3278
                     /gene="larE"
                     /locus_tag="P5516_05830"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HRT91992.1"
                     /translation="MNPLEQANQRLENAGITHPLKAKWQSLITELSSYPSAVIAYSGG
                     VDSSFLAYAAAQAMGGPMIAVTVFSPLEPAGLQQDVFRFVEQYGIKHDFLTHDPLQNA
                     KFQNNPPERCYLCKLGILNILWEYARDNHYAVLLEGQNADDQHEYRPGRQAVKETGTF
                     SPLAQNALTKAEIRWLARALGLPIWNQPSSPCLATRIPYGMTITEERLDQIASAENYL
                     HQRGFPVVRVRHLQHYASIEVEPDHLAKLLEMREDVVSYFKEIGFHHVALDLQGYRSG
                     SMDEGLSL"
     gene            3275..4477
                     /gene="larC"
                     /locus_tag="P5516_05835"
     CDS             3275..4477
                     /gene="larC"
                     /locus_tag="P5516_05835"
                     /inference="COORDINATES: protein motif:HMM:NF014072.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HRT91993.1"
                     /translation="MTCLYCDCFSGISGDMFLGALLDAGLPVETLSTQLKKLNLPEFQ
                     EAGKTKVFKGSLQATLLKLSIHQHAEDDQHHPHTHHRHFRDICQLIESSGLSEQVQQT
                     SKKIFGKLAEAEARVHGSTIEEVHFHEVGAVDSILDIVGAAIGLEYFGVQQVFSSALP
                     LGSGQVNTQHGLLPLPAPATMELIRMANAPVTGSKATSELVTPTGAAILSALAVFRQP
                     EMKIQRVGIGAGQRDLEWPNILRVFIGEAGDDFCTHLEIETNIDDMNPQVYGHVITKL
                     FQAGALDVYFTPILMKKNRPATKISVIARKEDEQNISDLILRETSTLGVRVKPIWRHE
                     SEREFRNIETRYGIVPVKLKVSGDDILQAAPEFDRCVQLAEQNQVPVLQVLKEVEAQV
                     TAHFKGQC"
     gene            4671..5432
                     /locus_tag="P5516_05840"
     CDS             4671..5432
                     /locus_tag="P5516_05840"
                     /inference="COORDINATES: protein motif:HMM:NF015669.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HRT91994.1"
                     /translation="MKNRILFSLLVLALVLSACGGNGSAVTPSLTGTHWVLEQINGKP
                     VIENVIPTLSFRGDQEVGGNASCNNFNGTYTGKDGALTIGPLATTMMACLEPAGLMEQ
                     EAAYLKALESAVEYRIEKGKLLIMDSAKAVVLTFAAQDMSLEGKTWNLTSFNDGQSLV
                     SLVPDTQITAEFKDGALSGSSGCNSYHGLFHQQEQKLSFDTFAVTMMACPEEGVMEQE
                     AAYLSSLAKVTHFSVEGNRLFLFDANDLIMAQFSQ"
     gene            5586..6917
                     /locus_tag="P5516_05845"
     CDS             5586..6917
                     /locus_tag="P5516_05845"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRT91995.1"
                     /translation="MSEHWNADVVIVGGGPTGSYLGWKLAQAGSDVLILETLPLEKVG
                     AHIEVIHLDQVRFDEFEIPHPLPPERMHLVPANKVWSVDKSGYFEVRYPFYVVNMPAF
                     VQRLHGYARESGGRVVGGAQVKEVIMEDGYCRGVRGTFNQQPFEARARIVVDASGLIS
                     AVRTKLPDDFGVENCPVPAEQTFYCTLELRDEIPAGYPVDNNSYLGSPGYWNKSYGDG
                     AVLGIIAPASVKNAWTALTQWRQATFGEPGKLVAKRAGCSPYRRSPNSYVGNGFVGVG
                     DCVYQNKAFSGEGITSGFTACKIASEVMVKALKNGDVSREGLWEYNVRYFHGQGAKFA
                     GVMMFLFPVNGLPNHEIDYLNRHNIIFCQDDYEYMNLNYELNLPAERWAAICEHLSDG
                     MKKGLFSENSLRLMNALYQKAGQVSAHYRAYPVNPSGIEAWSKVTREYWGY"
     gene            6926..8626
                     /locus_tag="P5516_05850"
     CDS             6926..8626
                     /locus_tag="P5516_05850"
                     /inference="COORDINATES: protein motif:HMM:NF027541.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GxGYxYP family putative glycoside hydrolase"
                     /protein_id="HRT91996.1"
                     /translation="MTETIKIFVLDVSHMSYAEQVTAACLQGLANRDQPRLFLDYGIY
                     DDPESRKTNQDALGEDLWVSKFRDAMGRQDEHNLNAYQKLYPIHVTKLNDLSSAVHQF
                     QDLCRGFVVWDPQQEDTVNLAVMFCSVENWLPVAPDLVEWSKQFGLKIEKDLRGCWQD
                     HVALHAWAHEQLFARCRPDRVANIEPHWRRPELLDYVVKEKIFTCNLSSKGSGRAFNF
                     GWKLLTLCLGGPTWLRNALYNVGLFRWVKNFALQKMSSDPETGLLISLLRKVARHPEG
                     IIFGWHTNRDDEFAFLALLSAIGLRLVPSHLAANFSFHSALPDPGPLKQFHVSEADVA
                     LEPEKDYLTFTYSDGDQLLLMSTAQLGGWRRKERGLVPFNWEMQPLLAELAPALLGLF
                     YSTLTPNDCLIAGPSGAGYIIPPLHDNLPEYLRRSAEVCAKADINVITSYYPDPPSKV
                     IQQHLQAPDNLIGYLSGYFYLKHRPVKFGHNKVFLCNLWPHLSQIKDSSDEVMAGVKK
                     ILDTPSSTPRFIGVHLFAYCTTVSDVYDFVRTLDPSKVKVVRADEFLLAAKKYSLQSK
                     "
     gene            8664..10040
                     /locus_tag="P5516_05855"
     CDS             8664..10040
                     /locus_tag="P5516_05855"
                     /inference="COORDINATES: protein motif:HMM:TIGR00792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015486 -
                     glycoside-pentoside-hexuronide:cation symporter activity
                     [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside-pentoside-hexuronide (GPH):cation
                     symporter"
                     /protein_id="HRT91997.1"
                     /translation="MTEKVTTHTKLLYGIADSGIAMLTSIVQFFLLFYYTDVALLDPA
                     MVGTALMVGKLTWDAINDVLFGYISDRTRSRWGRRRPYLLLLAIPLALSTWLMFSIPA
                     GLTGIPAFLIVLFTFLLFDSVHTFIAVAYTAITPELTSDYDERTSVTTVREVFTVVGY
                     ILGAALTTVVAGLYQKNLGFSLQGSYSAMGLTFGIYAAVVVLITAFGVKGKPASEIQP
                     AKMPAVKAFLVTFKNKPFVHLVTAFLIANVSFTLLTTLLPYYLKYQVQMEDQMPFVMI
                     SLLVTIGVFLYPMKMVVDRIGKGKGYALGLGIASLAILSIFFFPKGPTPLVYVASIVA
                     GMGLSAQWVCPWSMVPDVIEIDEIATGERREGIYYGMWNFITKLANAFAIAAAGWMLA
                     SFKYIPDAPQSDFTLLGIRILFSLVPVLMFAITIPLLIKYPITRESHARLVAELRGKI
                     SAGKETTT"
     gene            10579..10995
                     /locus_tag="P5516_05860"
     CDS             10579..10995
                     /locus_tag="P5516_05860"
                     /inference="COORDINATES: protein motif:HMM:NF012344.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT91998.1"
                     /translation="MEKAEKRWLYVLIIVFVIVNVVTLSSLIPWQSWQLWQNPEPAQV
                     VKVAYNDYTITMDTPVQIKAGEFVQFSATSADVTYGFGVFREDNSMVFQMQVLPGREN
                     QIIWKFDETGTFDVRSTEYSGPKHSDMVVRDAIVVK"
     gene            11008..12651
                     /locus_tag="P5516_05865"
     CDS             11008..12651
                     /locus_tag="P5516_05865"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRT91999.1"
                     /translation="MTTLGNMEKWTLRFAVVALLFLALSGFEGMLMRSQQYNIDSLHG
                     MEALLNNLRPGDVEPTTAELYYAMLSAHPIVGIYGFVYMAVFGAFYFLVPYLIQKPVK
                     HPKLVVANFWLQIIGVMTCWAAGFFGLFNAMYTLYWPLPVAFDRVPVVGSIFFMLGAA
                     AIMVNILLFSYNIFKTVLSKNGEGSYTMGQFLRAAFGIPRLLKLFGKEDKTAVNLDYN
                     GLPPFIVAVARGSIDTVINAIVLLSAGVLILVYGLFALFGHPLNPLAVDALIYKNIYW
                     WGLDMVADGNALIYTAGVWYLLVPLLTGRKLFGAGVVKTVIMADLLVSMGVWSHHLLG
                     DTSQPMWMKILSGQFITWGEYFTMGLTIFVSLMTIWKARPVKMSPAMKFTLGSMFGFV
                     MGGIAGLIQANVGLNMVFHNTQWVVALHAHTFLLTGVGTMLFSVIYTLVPLLTKLEFK
                     HPKLVDWHLWLWLIGSVSMAYAMGMAGSNGMIRRTLYTGGEFSTNTLAAFIGGSVVSI
                     GFVLFLINLVSTIGLKNVLSLVLPDRKTRQPDPLQAPAQ"
     gene            12911..14452
                     /locus_tag="P5516_05870"
     CDS             12911..14452
                     /locus_tag="P5516_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012398.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HRT92000.1"
                     /translation="MDEKTLNGHINHLRETFKSGITQPLAWRRRQLCMLNKMLKERSQ
                     EFETALSTDLRKSPYETYLTEIGLLQMEIDEALRELSNWSKPKSVPTPVFLHPARSYT
                     TFEPLGVMLIIGAWNYPLLLTIGPLVYAIAAGNAAVIKPPRTASAAFMAMANLLPLYL
                     DADAFFVIGDDTPNELILEQRFDKIFFTGSSATARIVMTAAAKHLTPVTLELGGKSPA
                     LVDRTADLKVAARRIAQGKFFNAGQTCVAPDYVLADEGILDAFVAELRSALYDFFGPS
                     PRESADFSRIINQKQFDQLLQYLSDGEVVVGGEWDREARYIAPTILKNVQLDAPVMQA
                     EIFGPILPVLPVKDMDAAIHFVQERDKPLALYIFSKDQATIDHVLSRTTSGGACVNEA
                     VNHVVVTGLPFGGVGNSGMGKYHGEWGFRELSNARAVLDHGTGFDPLLRYPPYNTAKM
                     AKLKTLMEMRTPAFLDGAMRLLIGKFGNLILRLVRPKAVEHSPLSSASAKKPQPAVQP
                     DCVET"
     gene            14676..15632
                     /locus_tag="P5516_05875"
     CDS             14676..15632
                     /locus_tag="P5516_05875"
                     /inference="COORDINATES: protein motif:HMM:NF019604.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HRT92001.1"
                     /translation="MVNEGQQYEVIIIGGGPAGATAAIYTARAGLKTLVLDKGLTAGA
                     LGITSKIANYPGLNAEVSGAQLLENMRAQARSFGAEFITDKVIGVNLLAEQKSVYGNN
                     GTYTGKAVIIATGSMGRGNRIKGEDELLGRGVSYCATCDAAFFRGQDVIVAGNSDEAI
                     EEALFLTRFVNRVHFLCPTPELKAPQPLIDEISANPKVQLYKGAALKEVIGTGKVQAV
                     RFTQRGLPEQELAVSGAFIYLQGGKPITDFLVGQLKLNDGGCLLVDEEYQTSIPGVFA
                     VGDILCNHIKQAVVAAADGAIAGMAAEKTLRGRKQMTVDWSK"
     gene            15666..16019
                     /gene="trxA"
                     /locus_tag="P5516_05880"
     CDS             15666..16019
                     /gene="trxA"
                     /locus_tag="P5516_05880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HRT92002.1"
                     /translation="MNEPVHVTDAAFEKAVLQSTLPVLVDFWAPWCGPCRMVAPILDK
                     IAKDYEGKVVVAKVNTDENNQWAMKYGVQGIPTMLFVANGKVVHQQVGALPEEMLRGT
                     LDQFLEVVAEDKKAG"
     gene            16230..17873
                     /locus_tag="P5516_05885"
     CDS             16230..17873
                     /locus_tag="P5516_05885"
                     /inference="COORDINATES: protein motif:HMM:NF013086.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HRT92003.1"
                     /translation="MQSSDSKVIVVGSGSAGFTAALAAKEAGLDPIIIESTGKLGGSS
                     AMSGGGAWIPNNPLMRRAGVFDSYEEARKYMDLTIGDVGPASSPERREAYLREGPQMV
                     EWLQSLGMRLHYGKGYADYYPELPGGSPLGRLVEPEPFDLKKLGQWKDKLNISISIPV
                     YTLDGVKIALSMRSLSTFLHTAYVIGIRTMGSMLLGKKLAGIGGALIGQLTFLALQRN
                     IPIWLNTPLVELISEGGEIKGVIVEKEGKRTELRARAVILAAGGFPHNNEMRQKYHPH
                     PITTDWTVAHKGNIGTAIQAGMAVGAATALMDDAWWGPCFIDSKGVSQFMIWERSFPY
                     SIIVDKSGKRFMNESASYVDCGHWQYERNKEVPAIPAYLIFDQHHHRYYPLGVGLPGA
                     EPKEYFESGMLTKANTLAELAQKIGVDPQGLEETVTRFNQFALNGKDEDFKRGDSAYD
                     RIYSDPKVKPNPNLAPLTQPPFYSVKVWPGDLGTKGGLLTNEHAQVLREDGSIIKGLY
                     AAGNTSASVMGHTYPGPGSTIGPAMVFGMLAGRHAATLE"
     gene            17994..19439
                     /locus_tag="P5516_05890"
     CDS             17994..19439
                     /locus_tag="P5516_05890"
                     /inference="COORDINATES: protein motif:HMM:NF014296.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HRT92004.1"
                     /translation="MPILTPGRRWQPIFYPFKKDKLGNRLLIRLEMLYKGPLWGCRQC
                     GNCVLQETAFICPMECPKGLRNGPCGGSTPEYCYVDETRPCIWYKIYERAFQSGREEL
                     LMEVLPPLDWDLVGGEQLGRLAGQVKKNGVLRVFTGLVSLDKAKRQRTWDGIFRPVRQ
                     PAWWRGDSVYHAPKYEGDASELERRLKAGEFVITAEVQPPQTASTNRLKQNIEMVSKH
                     ASAVNFTDGASATPRMSSWACSAIASQMGVEAVLQIAARDITRVSLQGTALGAAALGI
                     KNILCVTGDSNRLSPSPRGRMDIYDLDAIQMLWILREMRDEGRYLDERNIKYPPKYFL
                     GAVASPSASEVKFQALREHKKVNAGAQFFQTNILFDLGRFEEWLNALAARNILDKVYI
                     LAGISPLRSLRSAQFLNKEVPGVRVPDHILRRMEKADAAGNAQEEGIQVALELIEKLR
                     QYKEVNGLHLMTVGWEEIIPRLVHDAGLSQS"
     gene            complement(19615..21306)
                     /locus_tag="P5516_05895"
     CDS             complement(19615..21306)
                     /locus_tag="P5516_05895"
                     /inference="COORDINATES: protein motif:HMM:NF013584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HRT92005.1"
                     /translation="MTMYDLKSLNLPKLYGGVLKFFSAVTSNKLTRSLLVGSLLENGG
                     IPKLREMKFIEEPTNFPLVIPSERAWEPLELPQHIPSLSRPSRERLPFNMIRDFTAAY
                     LEGVVTPAQVAEEVIASIAASQQTTPPLNAFIASSAEDIRQQARDSTERLKNGQARSL
                     LEGVPLAIKDELDQVPYPTTVGTQFLGARPAAKDATVTARLRAAGAVLVGKTNMHEIG
                     IATNGENVHHGRIANPYDLQHDPGGSSSGSGAAVAAGILPAALGADGGGSIRVPASLN
                     GVVGLKPTFGRVSESGAAPLCWSVAHIGPLGASVEDVALIYQVIAGPDPAEPNTLQQP
                     PPTLRGWHTPDLKGVRIGIYADWFNHATQEVVALNYAVVSAFEKAGAQLIEVTIAELD
                     AMRVAHAITILSEMAACMRPYPQHWKDFAASTKLNLVLGQELTAYDYIQAQRMRTRAL
                     NIFAEIYREVDVILTPGTAMTAPIVPPQALSAGWSDLGTDTEMMRFVYPGNLTGLPAI
                     TFPVGYDANGMPVSMQAMGRHWEEHLLLRIAYNAELYTTRKLPQTYYGSRILGIS"
     gene            complement(21645..22907)
                     /locus_tag="P5516_05900"
     CDS             complement(21645..22907)
                     /locus_tag="P5516_05900"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92006.1"
                     /translation="MPPATLFYQRVLQGLRAYAGNVRAFSRNATLYLLSTILSSSAFG
                     VFRLLFNFYVLSLGYDQALVGNLVAASSMTALVAAIPMGYLADLLGRKLSLIGGGLVM
                     VVAICGMLLFPSPTIFILMNILMGLGQSISGVTMGPFLMENSGEKERTYLFSFSSGIS
                     MAASSVGNWIGGLLPTWLAVYAGGDATSASAYRLALTIVAVAASTAVVPLFLMTIQRG
                     KVVERSLFAPFTYFKSHPALLGKLVLPMLVTSIGAGLVMPFMNVFFRQVHGQSDSAIG
                     TLFAFGSLAMGVGLLIAPPLAEKYGKIQFVVITQALSIPFLALLGFSPLYWAAAAAYY
                     IRVALMNMSGPVYQTFVLEKVEPSARATVASLVSMASNFGWAFSPTISGYIQVKHGFA
                     PAFMLTMILYMLSISLYWGFFWTRARKT"
     gene            23087..24541
                     /locus_tag="P5516_05905"
     CDS             23087..24541
                     /locus_tag="P5516_05905"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TldD/PmbA family protein"
                     /protein_id="HRT92007.1"
                     /translation="MLDQIKQQISRVKALGASYVDTRWYPVEETNSLLMWNGNLKESS
                     ATFESGLGVRVLYHGAWGFSASSDTSDLAALYDRALDNARIASERLSLPVRLAEKDAI
                     QAKFVSPNKINPFEVPLSEKVAFLSEMDKKLDQPGVTQRASSLTFIRKQIIFVDSEGS
                     EIEKVITEVFAGLEVDGVDSEGGMYERKFSPFRRGDSRGWETIDLQHFSENAERIVRE
                     LNDTLNADPCPVDDRSVILLPGIMYLQTHETIGHALELDRILGYELAFAGGSFVTLDD
                     FGKLRYGSEKLTARADATLPNTPGSFGFDDDGIPAQDNLLIDRGLLVGAISGRQMVEE
                     ANARARRQIFHGSSGANRATSFYRVPIERMTNINIDPGNDGTLEEIIRNTEKGIVLDG
                     DRSWSIGSNREQFHFGTEAGWLVEDGRVTRAVKNATYKGATLPFWNSLTAVGDASTWE
                     VYYVPNCGKGQPNQIMQLGHGVPVCRFDNVRIGE"
     gene            24544..25929
                     /locus_tag="P5516_05910"
     CDS             24544..25929
                     /locus_tag="P5516_05910"
                     /inference="COORDINATES: protein motif:HMM:NF039907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallopeptidase TldD-related protein"
                     /protein_id="HRT92008.1"
                     /translation="MKDRILNTLDQLRRYALSKGYSVSIYFQEEDSSLMRFANSAISL
                     NTNEHLIRLNITACDGRKRASYGMITDLSRVEDMQRGVETAAEMVKHSMPLTYESTIP
                     VFREAFIDESGYDPSLAEMSNAEKLAFFNRVVADMESDEIKLSGVFSNGANTTAMVST
                     ATPYPLYIKTSDAQVSIVLAHQTLKWEIQSEQSAQKKDELSADAMRQELLCLMSHYMH
                     DTPQQLPLGVYDVVFGRAAIAEMLQFMSYIGIHGGLMKRGYSCLSKEDIGKKIFSEQF
                     TLIDDPTRLETFPFRRDLSGMTRQPFTLVENGIFKGFIWMQDDADEFGEQPTGHTIPH
                     LSLSMAGGSQAVGSLEDLFNQPRDHDLLYIPFLHYMNIVNPSKGILTSSSRFGAVLLK
                     KDGQTIIPYNVRLTQSLLDIFGDKVDWLSAETSAYNLSTSYDARNPTAFIVPRFARVN
                     GLEISHSNSSY"
BASE COUNT         6114 a         6962 c         6999 g         5896 t
ORIGIN      
        1 gtgatcgtct ttggtgttgc ttatggcatc gcccgcgctg aaaagcgccc atccgctcaa
       61 gacgacgccc aaaggtagac ggttgcgaga actttacaca ccaaaccaca gtgctgtaca
      121 tcgaatcgat gcaagggtga aggtcatctt cacccttgcg tttatcgttt ttttaaatct
      181 gaccccttcc ggcgcgtggc cagcgtatat cctctttttc acctggatgt tttctctatc
      241 tctgtcttcc cgcctgggga ttgccttcgt gctgaaaagg tctttgctcg cgttaccttt
      301 tgtgctcgcg gccttaccgc tggtctttac tggtcctgcc ccgctgggtc aaattcctgt
      361 actgaacacc ccttccttaa cttatagcgt ggtagggttt taccgcttcg ccagtattgc
      421 catcaaagcg tgggtttcgg taatggccgc ggtactgctc gccgcaacca cacgctttcc
      481 tgacctactt acagcgttct cccaactcaa ggttcccacg cttttcataa ctattattgg
      541 gttgatgtgg cgctatctat ttgtcatcag tgacgaggtc acccgcatgc tgcgagcgcg
      601 aaccagccgc agcgcaacag caccaaggtc aaaccgctca ggcgggtctc tgttttggcg
      661 ggcgcgcgtg accgggggca tggctgggaa tttgttcttg cgctcgattg aacgcagtga
      721 ccgggtatac gccgcgatgt tatccagagg gtatacaggt caacctgctg ctcagaaaac
      781 aacgcggtta cagaaaaaag acctgcttgt actcgctgca ggggttattc ttctggtcat
      841 attatggttg ttgggtgtaa tcaccggagg ttgaggattg gaaccagtca tcgaaatcag
      901 aaatctctcc ttttcttatc ctgatggaaa acaggccctc gcggggatca atttgttggt
      961 gcagccgggt gagaaggtcg cccttgtagg agccaatggg gctggtaaat caaccctctt
     1021 gtatcacctg aatggcattt ttacgggcag cgggatgatt cgcgtaaacc aacttgagtt
     1081 gaataaaaac aacctgccca gggtcagggc aatggttggt ctggtttttc aaaacccgga
     1141 tgatcagctt ttctctccaa ccgtttacga agacgtcgct tacgggccaa cctaccaggg
     1201 ctttgacccg cacatcgttc gggaaaaagt cgcgctggcg ttgaaagcgg ttcacatgga
     1261 agactatacg ctgcgtaacc cctatcacct gagcagcggt gagaagaaga gaatcgcaat
     1321 cgccaccgtt ctatcgatgc agccccagat cctggcgttc gacgaaccca ccgccgggtt
     1381 ggacccgcgc gccaggcgcg aattgattga cctgctgttg gaacttcagc aaaccatgat
     1441 catcgccaca catgatatga acatggtcga gcaactcacc cccagaaccg ttctcctcaa
     1501 tcatggaaaa atcgccgctg acgggccaag cctggacatc ctcagaaatg accggttgct
     1561 gatgaccaat gggttgatat aatcatttca atcggtcgaa gatctaccat cctatgcgaa
     1621 tatctcaggc aagaagaacg accggataag aggttgaatg gatcaagaaa cgctgcgcaa
     1681 actcctggag aatctgatca aaggagagct cactgtgccc caggtaattg atcagttgaa
     1741 aacgcttccc tacgaagggc tgaacgatta tgcaaaactg gaccatcacc gccccttgcg
     1801 aacggggtta cctgaggtga tcttcgccca gggtaaaacg ccgcaacagg tgttggatat
     1861 atttacacgg ttacaaaaga gtcaccacca ggtgctggcg acacgagtaa gccctggttt
     1921 atatgctgaa attcaagatc atctgagcgg tgtaacctat gattcaacgg cgcgcctatt
     1981 gttcaccagc cctgatacca ggcaggtgaa gcgccccggc attgtggtgc ttactgccgg
     2041 cacatcagat atccccgttg ccgaagaagc cgccatcacc gcagagctga tgggaaataa
     2101 ggtcgaacgc atttatgatg tcggcgtggc agggcttcac cgtttgatca gtcatttacc
     2161 tgtcttgcag aaagcccggg tggtgatcac cgtcgccggg atggaaggcg cgcttcccag
     2221 cgtggtcgcc gggttggttt ccgctccgct gatcgcggtt cccaccagcg ttggttatgg
     2281 cgcaagcttt caaggattag ccgcgttgct ggcaatgttg aacagctgtg ccagcggtgt
     2341 ggcggtggtt aatatcgata acggttttgg cgccggctgc atggctgcca aaatcaaccg
     2401 tttatccagc aacgagagcg gaaatgaacc ctcttgaaca agccaaccag cgcctggaaa
     2461 acgccggaat tacacacccc ctcaaagcca aatggcagtc tctgataact gaattgagct
     2521 cttatccgtc agcggttatc gcttattcag gcggcgtgga tagcagtttc ctggcgtatg
     2581 cagccgccca agccatgggc ggtccgatga tcgcggttac cgttttttca ccgctcgaac
     2641 ccgctggtct tcaacaagat gtgttccggt ttgtagaaca gtatgggatc aaacatgatt
     2701 tcctcacgca tgacccactg caaaatgcaa aattccaaaa caaccctcct gaacgctgtt
     2761 acctttgtaa actcggcatc ctcaatattt tgtgggagta cgcccgggat aaccattacg
     2821 ccgtgctact tgaaggtcaa aacgccgacg accagcatga atacagaccg ggacgccagg
     2881 ccgtcaaaga aacaggcaca ttcagccccc tggcgcagaa cgcgctcacc aaagcggaaa
     2941 tccgctggct tgccagggcg ttagggctac ccatatggaa ccagccaagt tctccctgcc
     3001 tggctacccg aatcccctac ggcatgacca tcacggagga gaggcttgat caaattgcca
     3061 gcgctgaaaa ttacttgcat caacgcggtt ttcccgttgt tcgggtccgc catcttcagc
     3121 attacgcgag tattgaagta gaacctgacc acctggcgaa gctgttggag atgcgagagg
     3181 atgtggtaag ctattttaaa gaaatcggtt tccatcatgt tgctttagat ctgcagggat
     3241 accgatctgg cagcatggac gaaggattgt ctctatgacc tgtttatatt gcgattgttt
     3301 ttctggaatc agcggggata tgttcctggg cgcgttgctc gacgcgggtt taccggttga
     3361 gactctatct acgcagttga aaaagcttaa cctccctgag tttcaagaag cgggtaaaac
     3421 gaaagtgttc aagggttctt tgcaggcaac gcttttaaaa ttgagtatcc atcaacacgc
     3481 tgaagacgac caacaccatc ctcacacgca ccatcggcat tttcgcgaca tctgtcaatt
     3541 gatcgaatcc agcgggttgt cagaacaggt gcagcaaacc agtaaaaaaa tctttggcaa
     3601 gctggctgaa gccgaagcca gggttcatgg ctcgaccatc gaagaggttc atttccatga
     3661 agtcggcgcg gttgattcca ttttggatat cgtcggcgcg gcaatagggc tggaatattt
     3721 cggtgttcaa caggtttttt cttccgcact accgctggga agcgggcagg tcaataccca
     3781 gcatgggctg ctcccgctgc ccgcgccagc cacaatggag ttgattcgta tggctaacgc
     3841 gccggtcacg ggttctaaag ccacaagcga actggtcact cctacaggcg cggctatcct
     3901 ttcggctctg gctgttttcc gtcagcctga aatgaaaatt caacgtgtgg gaattggcgc
     3961 agggcaaaga gatttggagt ggccaaacat cttacgagtt ttcatcggcg aagcaggaga
     4021 tgatttctgc acccacctgg aaattgaaac gaatatcgac gacatgaacc cgcaggttta
     4081 cgggcacgtg atcacgaaac tgttccaggc tggggcgctg gacgtttatt tcacgcccat
     4141 cttgatgaaa aaaaaccgcc cggcgacaaa aatctcagtc atcgcgcgca aagaagatga
     4201 gcagaacatc agcgacctta tcctccgcga aaccagcact ttaggcgtca gggttaagcc
     4261 catctggagg cacgaatcag agcgcgagtt tcgcaatatt gaaacccgtt acgggatagt
     4321 gccggtgaag ctcaaggtta gtggggatga tatcctgcag gctgctccgg agtttgatag
     4381 atgtgttcaa ctggctgaac aaaaccaggt gccggtccta caggtgctga aagaagtaga
     4441 agcacaggtt accgctcact tcaaaggtca atgttgatgt tttctgtgtt tgcgcgatgg
     4501 acttatccgc aatttagatt gagttttttt aaaaggagga atttcttctc atccatttct
     4561 tgaaccagcc tgcagaggaa aaacaatggg gtcactaagg cgatcggccg ggaactaatt
     4621 ccttaccaaa atcgtcttat tcacaacaaa acaacataag gagtagttag atgaaaaata
     4681 gaatcttgtt ttctttactg gtgcttgcgt tggttttatc cgcctgcggt ggaaatggga
     4741 gcgctgtcac gccttctttg accggtactc actgggtttt agaacagatc aacggcaagc
     4801 ccgtgattga gaatgtaatc ccgaccctgt cttttcgtgg tgaccaagaa gtgggcggta
     4861 atgcctcatg caacaatttc aacggcacgt atacaggtaa agatggagcc ctgaccattg
     4921 gcccgctggc cactaccatg atggcctgcc ttgagcccgc cgggttgatg gagcaggaag
     4981 ccgcttacct caaagccctg gaatccgccg tggaataccg gattgagaaa ggcaaattac
     5041 tgatcatgga tagcgcgaaa gcggttgtgc tgacctttgc tgcgcaagac atgagcctgg
     5101 aagggaagac ctggaacctg acctctttca acgacggaca gagcctggtg agtttggtgc
     5161 cagatacaca aataacagcc gagttcaaag atggtgcgct ctcaggttca agcggctgca
     5221 atagttacca tgggttgttc catcagcagg agcagaaact gtccttcgac acgtttgcgg
     5281 taacaatgat ggcttgtcct gaggaaggtg tgatggaaca agaagctgcg tacctgagca
     5341 gcctggccaa agtgacgcat ttcagcgtcg aaggcaaccg cttgttcctg ttcgatgcca
     5401 atgacctgat catggctcaa ttcagccagt agggtcgtgc ctggaatcac aaaaacctgc
     5461 ccctcgcggc aggttttttt atttccatcg tttgatggtt gtgctatttc ccggtttctc
     5521 ttatactcat ctccagatat aacacctcaa ttgatcaact ctatatcaac gcatggagat
     5581 tcacaatgtc tgagcactgg aacgcggatg tggtaattgt ggggggagga cctacagggt
     5641 cttacctggg gtggaaactg gcgcaggcag gaagcgatgt tctcattcta gaaacgctgc
     5701 ctctcgaaaa ggttggcgcg catatcgagg tcattcatct cgaccaggtg cgtttcgatg
     5761 agttcgaaat ccctcacccg ctgcctcctg aaagaatgca cctggtacca gccaacaaag
     5821 tttggtcggt ggataagagc gggtattttg aagtgcgcta cccgttctac gtggtcaata
     5881 tgcctgcttt tgtgcagcgg ctgcatggct acgcgcgcga atctggcgga cgggtcgtag
     5941 gcggagccca ggtaaaagag gtgatcatgg aagacgggta ttgccgcggt gtgcggggga
     6001 ctttcaatca acagccgttt gaagcccgcg ccagaatcgt tgtcgatgct tcaggtctca
     6061 tcagcgccgt gcgcaccaag ctcccagatg atttcggggt ggagaattgc ccggtgcccg
     6121 cggagcagac tttttattgc acattggaac tgcgtgacga aatacccgcc ggttaccctg
     6181 tagacaataa ttcctacctt ggctccccag gctactggaa caagtcttat ggtgatggtg
     6241 cagtgttggg aattatcgcc cccgccagcg tgaaaaacgc gtggacggcc ttaacccaat
     6301 ggcggcaggc gacttttggc gaaccaggca agctggtagc caaacgcgct ggctgctctc
     6361 cgtaccgccg ttcccccaat tcttatgtag ggaatggctt tgttggcgtc ggcgactgtg
     6421 tttaccagaa caaggcgttc tccggagagg gtatcaccag cgggttcacc gcgtgtaaaa
     6481 tcgccagcga agtgatggtg aaagcgttaa aaaatggtga cgttagccgt gaagggcttt
     6541 gggaatacaa tgttcgctac ttccacggtc agggcgcaaa attcgccggc gtgatgatgt
     6601 tcctgttccc ggtgaacggg ctgcccaacc acgagatcga ttatcttaat cggcataaca
     6661 tcatcttttg ccaggatgac tacgagtaca tgaatctcaa ttatgaactg aacctcccgg
     6721 cagaaagatg ggccgccatt tgtgagcatt tatcagatgg catgaagaaa gggttatttt
     6781 cagaaaacag tctgcggtta atgaacgccc tgtatcagaa agctggccag gttagcgcgc
     6841 attaccgggc atatcccgtc aacccctccg gtattgaagc gtggagcaaa gtcacgcggg
     6901 aatactgggg atactgacca ctgccatgac cgagacaatc aagatattcg ttttagatgt
     6961 cagccacatg agctacgccg agcaggtgac tgccgcctgc ctgcaaggat tagccaaccg
     7021 tgaccagccg cgcctgttcc ttgattatgg tatctacgat gaccccgaat ccagaaagac
     7081 caaccaggat gcactgggcg aagacctgtg ggtaagcaaa tttcgggatg cgatgggcag
     7141 gcaggatgag cacaacctga acgcttacca gaaactttac cccattcatg ttacgaaact
     7201 gaatgatctc tccagcgccg ttcatcagtt ccaggacctc tgccgtgggt ttgttgtttg
     7261 ggacccccag caggaggata ctgtgaacct ggcggtgatg ttctgcagtg tggagaactg
     7321 gctccccgtc gctcctgacc ttgtggaatg gtcgaagcag tttgggctca aaattgaaaa
     7381 agacctgcgc ggctgttggc aagaccacgt cgcgctccac gcctgggcgc atgagcagct
     7441 ctttgcccgc tgccgacccg atcgggtggc caacattgaa ccacactggc gccgcccgga
     7501 gctattggat tatgtggtga aagagaaaat cttcacctgt aacctatcca gcaaaggcag
     7561 tggccgagct ttcaacttcg gctggaagct gctcaccctt tgcctgggag gtccaacctg
     7621 gctgcggaac gcgttgtata acgttggcct ttttcgctgg gtgaagaatt tcgccttaca
     7681 gaaaatgtct tccgaccccg aaacgggtct gctgatctcg ctattacgaa aagttgcccg
     7741 gcacccggag ggcatcattt ttggctggca caccaatcgt gatgacgaat tcgcttttct
     7801 ggcgttgctg agcgccatcg gtctgcggct cgttccctcg cacctggcgg cgaatttctc
     7861 tttccatagc gcgttgcccg acccggggcc attaaagcaa ttccatgttt ctgaagccga
     7921 tgtggcgctc gagcctgaga aagattacct gaccttcacc tactcggatg gtgaccaatt
     7981 gttattaatg agcaccgccc aactgggcgg gtggcgcaga aaggagcgcg gtctcgttcc
     8041 tttcaattgg gaaatgcagc ctctcctggc ggagctggcc ccggctttgt tggggctgtt
     8101 ttacagcaca ctgacaccca acgattgcct gattgccggt ccgtctggag cagggtacat
     8161 catccctccc ctgcatgaca acctgccgga gtatttacgc cgctcagccg aagtttgcgc
     8221 gaaggcggat atcaatgtca tcacgtcata ttatcctgac ccgcctagca aagtcattca
     8281 gcagcacctg caagcgccag acaacctgat agggtatctc tcagggtatt tttatctcaa
     8341 gcatcggccg gtgaagttcg gtcataacaa ggtctttctc tgcaaccttt ggccgcatct
     8401 atcgcagatc aaggattcaa gcgatgaggt gatggcaggc gttaagaaaa tcctggatac
     8461 gccatccagt actccgagat tcatcggggt gcaccttttt gcctactgca ccacagtaag
     8521 cgatgtttac gattttgtgc gaaccctgga cccaagcaaa gttaaagtcg tccgggctga
     8581 cgagttctta ctggcagcga aaaaatattc ccttcaatcg aaataaacca tcttaattac
     8641 aaatgaacca aaaggaagcc aacatgacag aaaaagttac tacgcacacc aagcttttat
     8701 acggtatcgc ggattcaggt atcgcgatgc tgacgtcgat cgttcaattc tttctccttt
     8761 tttattacac ggatgtcgcc ctgctggacc cggccatggt agggacagcg ctgatggtgg
     8821 gtaaactcac ctgggacgcg atcaatgatg tcctctttgg ctatatttcc gatcgcacca
     8881 ggtctcgttg ggggcggcgc agaccctacc ttctcttact ggcgattccc ctggcgctat
     8941 ccacctggct gatgttctca attccagccg gactcaccgg aataccggct tttctaatcg
     9001 tattgttcac cttcttattg ttcgattcag ttcatacctt tatcgcggtg gcgtacaccg
     9061 ccatcacccc cgagttgacc tctgattatg atgaacgcac atccgtcacc acggtccgcg
     9121 aagttttcac cgttgtcggg tatatccttg gcgcggctct gaccacggtg gtggcaggcc
     9181 tgtaccaaaa gaacctgggg ttttctcttc agggcagtta tagcgcaatg gggttgacct
     9241 ttggcattta cgctgctgtg gtggttctaa ttactgcttt cggcgtcaaa ggaaaaccag
     9301 cgtcagaaat acagcccgcg aaaatgcctg ccgtaaaagc cttcctggtc acatttaaaa
     9361 ataaaccttt tgtgcacctg gtgaccgcct ttttgattgc caatgtgagt ttcacactct
     9421 tgaccaccct gctgccatac tacctgaaat accaggttca aatggaagat caaatgcctt
     9481 tcgtgatgat ttcactcctg gttaccatcg gggtgttcct ctacccgatg aaaatggtcg
     9541 tggaccggat tggaaagggc aaagggtatg ccttggggct gggtatcgct tccctggcaa
     9601 ttctcagtat cttctttttc ccgaaaggtc caacccccct ggtgtatgtt gcgtcgatcg
     9661 tcgcgggaat ggggctctcc gcgcagtggg tctgcccgtg gagcatggtg cctgatgtga
     9721 tcgagattga cgagatcgcc accggcgagc gaagagaagg aatttactac gggatgtgga
     9781 acttcatcac caagttagcc aacgcgttcg ccatcgccgc ggctggttgg atgctggcgt
     9841 cgttcaaata tattcctgat gcccctcaat ccgatttcac cctgctgggt atccgcatcc
     9901 tgttcagcct ggtgcccgtt ctcatgttcg ccatcaccat ccccctgtta atcaagtacc
     9961 ccatcacgcg cgaaagtcac gcccggctgg tcgcggagct gcggggcaag atcagcgcgg
    10021 ggaaagaaac caccacctga aacaccgttc tcatgcggcg gcggcaagcc aaagccggcc
    10081 gggttatcca acctcaagcc gacggtttac aagcaggttc caaacagcca gtggacgtga
    10141 ctggttgttt tcctgtctaa tcttttaata agagcagtgt tcggattgtt aaatacctta
    10201 aaataacctt tcttttcttg ggtaacatcc ctacatagaa taaaactact tcttttggct
    10261 gaatatacca atcaacagga gcgctttttt tactaccggg taatcccagg cttgatcatt
    10321 gaccgttcag gcagaacaca ttcccgaatt ttcggatggg tgaaatcgaa cgttcgtatg
    10381 catgaaaggt ttcccaaaat ccatatcttt tttgtttcat ttttgttata ttatttacac
    10441 gaaatccgct gattttcaat aaggaggata agatgcatct ttctcaagtg gtgtggtgga
    10501 tctatactgc actgatcgcg ttggccgctt tgttttttct tatctttgcc cacctggtga
    10561 aggagaaagg caaataaaat ggaaaaggcc gaaaaaagat ggttgtacgt tttgatcatt
    10621 gtgtttgtta tcgtcaatgt cgtcaccctt tcttctctta tcccgtggca gtcgtggcag
    10681 ctctggcaaa accctgaacc cgcgcaggtg gtgaaggttg cctataacga ctacaccatc
    10741 accatggata caccggtaca gatcaaggct ggcgagttcg tgcaattctc cgcgacctca
    10801 gcggatgtga cctacggctt cggcgtgttc cgcgaagaca acagtatggt ctttcaaatg
    10861 caggtgctgc ccggcaggga gaaccagatc atttggaagt tcgatgaaac gggcaccttt
    10921 gacgtccgtt ccacagaata ctccgggccc aaacattccg atatggtcgt gcgcgacgcg
    10981 attgtggtga aataaggagc aagaaaaatg accactcttg gcaacatgga aaaatggacg
    11041 ctgcgtttcg cagtggtggc tctcctcttc ctggcgcttt caggtttcga gggcatgctg
    11101 atgcgttctc agcaatacaa tattgattcg ctgcacggta tggaggcgct gctcaacaac
    11161 ctcagacccg gcgatgtcga acctaccacc gccgaactgt attacgccat gctctccgct
    11221 catccgatcg tgggtattta tggcttcgtg tatatggcag tcttcggggc gttttatttt
    11281 ctcgttccgt atctcatcca aaagccggtc aaacatccta agctggtggt tgccaatttc
    11341 tggctgcaga tcatcggtgt gatgacctgc tgggcggcgg gattcttcgg cttgtttaac
    11401 gccatgtaca cactttactg gccactgcca gtggcgtttg accgcgtacc ggtcgtcggc
    11461 agcatcttct tcatgttggg cgcggcagcc attatggtga acatcctgct cttttcatat
    11521 aacatattta agacggtgct gagcaagaac ggtgagggct cttacaccat ggggcagttc
    11581 ctgcgcgccg ctttcggtat accgcgttta ctgaaactgt tcggcaaaga ggataaaacc
    11641 gccgtcaacc tggattacaa cgggctgccc ccctttatcg tggcggtggc gcgtggctcc
    11701 attgatacgg tcatcaacgc catcgtgctg ctctcagccg gggtactcat cctggtttat
    11761 ggtctgttcg cgctcttcgg tcatcccctc aacccgctgg cagtggacgc gctcatttac
    11821 aagaacattt attggtgggg gttggatatg gtcgccgacg gcaacgcgct catctacacc
    11881 gccggggtgt ggtacctgct ggtgccgctg ctcaccgggc gcaaactttt tggcgccggt
    11941 gtggtcaaaa cggtcatcat ggcagacctg ctggtctcaa tgggggtatg gagccaccat
    12001 ttgctgggag atacctcgca gccgatgtgg atgaaaatcc tctccggtca atttatcacc
    12061 tggggcgagt acttcaccat ggggctgacc atcttcgtgt cgctcatgac catctggaaa
    12121 gcgcgcccgg tgaagatgag cccagcgatg aaattcaccc tgggttccat gttcggtttt
    12181 gtgatgggcg gaatcgccgg gctgatacag gcgaacgtcg gtttgaacat ggtctttcat
    12241 aacacgcagt gggtggtggc gctgcacgcg cacaccttcc tgttgactgg cgtgggaacg
    12301 atgctgttct cagtgatcta cacgctggta cccctgctca ccaaactgga gtttaaacac
    12361 cccaaactgg ttgactggca cctgtggtta tggctcatcg gctcggtttc aatggcctac
    12421 gccatgggca tggcgggatc aaacggtatg atccgccgca ccctctatac cggaggggag
    12481 ttcagcacca atacgctggc ggcgttcatt ggcggttcgg tggtcagcat cgggtttgtg
    12541 ctcttcttga tcaacctggt gagcaccatc ggcttaaaga atgtgctttc cctggtcctg
    12601 ccggatagaa aaaccaggca gcctgacccg cttcaggcac cggcccaata atttgcaccc
    12661 aattaaagga taagaaaccg actgccgcgc aaacctgcgg cggtcggtcc atatgggtga
    12721 gttatcgcgc cagggatcag ggcagccaat cacgactgaa gcgtgagccg cagccaacga
    12781 acgggtataa ttccctctgg cttagcatcg ctgcaagggg tgatatctca actgccattt
    12841 cctcgctgca gcaatcattc attaaggagt atgggcgctc cgcctgatgc agtagcgcct
    12901 ggcaaccatc atggacgaaa aaacactgaa cgggcatatc aaccatctgc gcgaaacttt
    12961 taaaagcggg ataacccagc cgctcgcgtg gcgccgccgg caattgtgca tgctcaacaa
    13021 aatgctcaaa gaacgcagcc aggaatttga aaccgcgctt tccacagacc tccgtaaaag
    13081 cccttatgaa acttacctca cagaaatcgg gctgctgcaa atggaaattg atgaagcgct
    13141 gcgcgaactg agcaactggt ccaagccaaa aagcgtcccc acgccggtgt tcctccatcc
    13201 agcgcgcagc tacaccacct ttgaaccgct gggagtgatg ttgatcatag gagcgtggaa
    13261 ttaccccctg ctgctgacca ttggcccgtt ggtctacgcg atcgccgccg gtaacgccgc
    13321 ggtcattaaa cctcctcgta ccgccagcgc cgccttcatg gccatggcca atctcctgcc
    13381 actctacctg gacgccgacg ctttttttgt gattggtgac gacaccccca atgagctgat
    13441 tctggaacag cggtttgaca agatcttttt caccggcagc tcagccactg ccaggattgt
    13501 gatgacagcg gcggcgaagc atctcacccc ggtcaccctg gaactcggcg gtaaaagccc
    13561 cgccctggtt gaccgcacag ccgatctcaa agtcgccgcg cggcgtatcg cccagggaaa
    13621 attcttcaac gctggccaaa cctgcgtggc gcctgactat gttcttgccg atgaaggaat
    13681 cctggacgcg ttcgtggctg agttgcgcag cgccctgtat gactttttcg gaccttcgcc
    13741 ccgcgagagc gccgattttt cccgcattat caatcagaaa caattcgacc agctgcttca
    13801 atacctgagc gatggcgaag tggttgtggg tggtgaatgg gaccgagaag cgcgctatat
    13861 cgctcccact attttgaaga acgttcagct tgatgccccg gtgatgcagg cggagatttt
    13921 tggtccaatt ctgcctgtgc taccagtgaa ggatatggac gcggcgattc acttcgtgca
    13981 agaacgtgat aaacccctgg cgttatatat cttctcaaaa gaccaggcaa ccattgacca
    14041 tgtcctgtcg cgcaccacct caggcggcgc ctgtgtgaat gaagcggtca atcacgtagt
    14101 ggtcaccggt ttgccatttg gtggggttgg caacagcggt atggggaaat accacggtga
    14161 atggggtttc agagaattat ccaacgcgcg tgcagtgctc gatcatggaa ccggctttga
    14221 ccctttgctg cgctatccgc cgtacaacac agcaaaaatg gccaagttga aaaccctgat
    14281 ggaaatgaga acgcccgcct ttctggatgg ggcgatgcgt ttgctgatcg ggaagtttgg
    14341 caacctcatt ttgcgactgg tacggccaaa ggcggtagaa cactcgccgc tttcttcggc
    14401 ttccgcaaag aaacctcaac ctgctgtaca gcctgattgt gttgaaacgt aattctctca
    14461 cccgttctca ttggtctacc agtcaggctg catccagaac gagcgggcca acagggctgc
    14521 cggtctggtg accttcaacc gcttcttcct gaccttgtac tgctgggttt tttttgcatt
    14581 tgagatttta ttcacattca catgtcactt tttcgcgtct tccgcatctg aaggatggat
    14641 gcaaataaca acacttttta attgtggaac tatttatggt aaatgaaggt caacaatacg
    14701 aagtaatcat catcggcggc gggcccgccg gggcaaccgc ggcaatttac acagcccgcg
    14761 ccgggttaaa gacgctggtg ctggataaag gtttgaccgc cggtgcgttg gggatcacca
    14821 gcaaaatcgc taattacccg ggattgaacg cggaggtgag cggagcgcag ttgcttgaaa
    14881 acatgcgcgc ccaggcgcgc tcgttcggtg cagagttcat cacagataaa gtgatcggcg
    14941 tcaacctgct cgcagagcag aaaagtgttt acggcaacaa cggcacctac accggtaaag
    15001 cagtgatcat cgccaccggc tcaatgggac gcggcaaccg catcaaaggc gaagatgaac
    15061 tgctggggcg cggtgtttct tactgcgcca cctgtgacgc ggccttcttc cgtggtcagg
    15121 atgtgatagt ggcgggcaac agcgatgaag ccatcgaaga ggcgcttttc ctcacccggt
    15181 ttgtcaaccg cgtgcacttc ctttgcccca cgccagagct taaggcgccg caacccctca
    15241 tcgatgaaat ctccgccaac ccgaaggttc agctatacaa gggggcagcc ctaaaagaag
    15301 tgattggtac gggtaaagtg caggctgtga gattcaccca aagagggctg cctgaacaag
    15361 aactcgccgt gagcggcgcc ttcatttacc tgcaaggcgg aaaaccgatc accgatttcc
    15421 tggttgggca gttaaagctc aacgacgggg ggtgcctgct ggtagatgag gaatatcaaa
    15481 ccagtattcc tggtgtgttc gctgtgggag acatcctgtg caatcacatc aaacaggcgg
    15541 tggtcgcggc tgctgatggg gcgatcgccg ggatggcggc agagaaaacc ctgcgcggac
    15601 gcaaacagat gactgtcgat tggtcaaaat agattaatat tacctatcct ggaggagaaa
    15661 aaaaaatgaa cgaacctgta catgttaccg atgctgcatt tgagaaagcc gttttacaat
    15721 ctactctgcc ggtactggtg gatttctggg cgccctggtg tggtccctgc cgcatggtcg
    15781 cccccatcct ggacaaaatt gccaaggatt acgaaggcaa ggtcgtggtc gccaaggtta
    15841 acacagatga gaacaaccag tgggcgatga aatacggcgt gcagggtatc cctaccatgc
    15901 tttttgtcgc caatggtaaa gtggtgcatc agcaggtcgg cgctttgccc gaagagatgc
    15961 tacggggcac gctggaccag ttccttgaag tcgtcgccga ggataaaaaa gccgggtaag
    16021 caggcaatcc atcaccccgc aatgcacttt aaaaaggcca cctttcggtg gtttttttat
    16081 tgtttgtttc ttcgctggtg agggcacagg ggcgaatacc gcatgtcctt aagggttgat
    16141 ggtaaaatat tcagacaaat attatgtgat ttgacatata taagcgtgcg ttgtggattt
    16201 tgtagatcga ccctgaggag gagagaaaaa tgcagagcag tgattcaaaa gtcatcgtgg
    16261 ttggttcggg ctcagccggg tttacggcag cgctggctgc caaagaagcc ggtcttgatc
    16321 ccatcatcat cgagagcacc ggcaaactgg gaggttcatc cgccatgtct ggcggagggg
    16381 cgtggatccc caacaacccg ctgatgcgca gagccggagt ctttgactcg tacgaagagg
    16441 cccgcaaata tatggacctg accatcggcg atgtagggcc agccagctcg cctgaaaggc
    16501 gtgaggcgta tctgcgcgaa ggaccgcaaa tggtggagtg gctccagagc ctgggtatgc
    16561 gcctgcacta cggcaagggc tacgcagatt actaccccga gctgcccggc ggttcgcccc
    16621 ttggtcggct ggtggaacca gaaccttttg acctgaagaa actgggccag tggaaagaca
    16681 agctcaatat ctccatatcg atcccagttt acacgctgga tggcgtcaag atcgcgcttt
    16741 ccatgcgttc gctctccact tttttgcaca ccgcttatgt gattggcatc cgcaccatgg
    16801 gcagcatgct tctgggcaag aagctcgcgg gtatcggcgg cgcgttgatt gggcagctca
    16861 cattcctggc cctgcagcgc aacattccca tctggttgaa tacccccctg gtcgaactca
    16921 tcagtgaagg cggcgagatc aaaggcgtga ttgtcgaaaa agagggcaag cgcactgaat
    16981 tgcgcgccag agcggtcatc ctggcggcag gtggattccc acacaataac gaaatgcgcc
    17041 agaagtacca cccacacccc atcaccactg actggacagt cgcgcacaaa ggcaatatcg
    17101 gtaccgccat ccaggctggc atggccgtgg gcgcagcgac cgcgttgatg gatgatgcct
    17161 ggtggggacc ctgctttatc gacagcaagg gcgtatcgca gttcatgatc tgggagcgtt
    17221 ccttccccta ttccatcatt gtagataaat ccggcaagcg ctttatgaac gagtctgctt
    17281 cttatgtcga ttgtggtcac tggcagtacg agcgcaataa ggaagttcct gccatccccg
    17341 cgtacctcat cttcgaccag catcatcacc gctactaccc gctgggggtg gggctgccag
    17401 gcgcggaacc caaagagtac tttgagtctg ggatgctgac caaagcaaac acgctggccg
    17461 agctggcgca gaagattggc gtcgacccgc aggggcttga agaaacggtg actcgcttca
    17521 atcagttcgc gctgaatgga aaggatgagg atttcaaacg cggggacagc gcttacgatc
    17581 gtatttacag cgatcccaag gtcaaaccca accccaacct ggcgccgctg acgcaaccac
    17641 cattctattc agtcaaagtg tggcccggag acctgggcac caagggcggc ctgcttacca
    17701 atgaacacgc gcaggtactg agagaggatg gcagcatcat caagggcctg tacgcggcag
    17761 gcaacacttc cgcttcggtc atggggcaca cctatccagg acccggttcc accatcggcc
    17821 cggccatggt tttcggcatg ctcgccggca ggcacgccgc cacattagaa taaacgccag
    17881 cgccaataag ggtgatgtgc gcacattgga gcatgcgtgg catgctcctt cttttcttct
    17941 tgacgtgact ggaaacccca atataatcag gagaacggta aaggaaatcc actatgccca
    18001 ttttgacccc tggacgccgc tggcagccga ttttttaccc gtttaaaaaa gataaactgg
    18061 gcaaccggct gctcatccgc ctggagatgc tgtacaaagg ccccttatgg ggctgccggc
    18121 agtgcggtaa ttgcgtgctt caggaaacag ccttcatatg cccgatggaa tgcccgaagg
    18181 ggctgcgtaa tggcccatgc ggcggttcca cacccgagta ctgctacgtg gatgaaaccc
    18241 gcccatgcat ctggtataaa atctacgaac gcgctttcca aagcggtcgt gaagagctgc
    18301 tgatggaggt gctgccgccg ctggattggg acctggtggg cggagaacag ttggggcgcc
    18361 ttgccggcca ggtaaaaaag aacggagtgc tgcgggtgtt caccggcctg gtttcactcg
    18421 ataaagccaa acggcagcgc acctgggatg gtattttccg tccggtgcgt cagcccgcct
    18481 ggtggcgcgg cgattctgtg taccatgcgc caaaatacga gggggatgct tcagagctgg
    18541 aacgccggtt gaaagcaggc gaatttgtga tcaccgccga ggttcagccg ccgcaaacgg
    18601 cttctaccaa ccggcttaaa cagaacatcg aaatggtcag caaacacgcc agcgcggtga
    18661 atttcaccga tggcgcctca gccacgccgc gcatgtcttc gtgggcatgc tcagccatcg
    18721 cgtcgcagat gggcgtggaa gcggtgttgc aaattgcggc gcgcgatata acccgtgtga
    18781 gcctgcaggg cacagcactg ggggcagcgg ccctgggcat taagaacatc ctttgtgtca
    18841 ccggagatag caaccgcctg tcaccctctc cgcgcggcag aatggatata tacgacctgg
    18901 acgcgatcca gatgttgtgg atattgcgcg aaatgcggga cgaaggcagg tacctggatg
    18961 aacgcaatat caagtatccg ccgaaatatt tcctcggtgc tgtggcttca ccctcggcat
    19021 ctgaggtcaa atttcaagcg ctgcgggaac ataagaaggt caacgctggc gcgcagttct
    19081 ttcagaccaa tattctattt gacctgggtc gttttgaaga atggttaaac gccttggcag
    19141 cgcgaaatat tcttgacaag gtgtatatcc tggcgggaat ttctccctta cgctcgctgc
    19201 gcagcgccca attcttgaac aaagaagtcc ctggtgtgcg tgtgcctgac catatcctcc
    19261 ggcgcatgga aaaagcagac gcggctggca acgcccagga agagggcatt caggttgcgc
    19321 ttgaattgat tgaaaagctc cgtcagtaca aagaggtgaa cggcttgcac ctcatgaccg
    19381 tgggctggga ggaaatcata cccaggctgg ttcatgacgc gggcctctca caatcgtaac
    19441 gatcggagag atcatctcag gcgtgcttga agacagaagg atatcgtgac cagatcagag
    19501 cagcgctgtt cgcccgcaca ttcttaaaag aatctaacac ttcgcggtcg accgcaaccg
    19561 ggaatcaagc atcccgcgag tcaatcgcgg gatcttcatt atttgccctg gaattcagga
    19621 aatacccagt atgcgcgagc cgtaataggt ttgcgggagt ttgcgcgtgg tgtacagctc
    19681 cgcgttatag gctatacgca gcaacaggtg ctcttcccag tgccgaccca tcgcctgcat
    19741 gctcaccggc atcccgttgg catcataacc caccgggaaa gtgatcgccg gcaaaccagt
    19801 gaggttgcca gggtaaacaa agcgcatcat ttcagtgtcc gtgcccagat ccgaccagcc
    19861 cgcgctgagc gcctgaggag gaacgattgg cgcggtcatc gctgtgccag gagtgaggat
    19921 gacatccacc tcacggtata tctcggcgaa gatgttcaag gcgcgggtgc gcatacgctg
    19981 cgcctggata taatcgtaag cggtcaactc ctgccccaac accagattca gcttcgtact
    20041 ggcggcgaaa tctttccagt gctgcgggta aggccgcatg caggcggcca tctcacttaa
    20101 tatggtaatg gcgtgagcta cacgcatcgc gtcaagctcc gcgatggtca cttctatcaa
    20161 ctgcgcgccg gctttctcaa acgctgatac cacggcgtaa ttcaacgcca caacctcctg
    20221 ggtggcgtgg ttgaaccagt cagcgtatat gccgatgcgc acgcctttca ggtcgggtgt
    20281 atgccagccg cgcagggtgg gcggaggttg ttgcagcgtg ttgggctccg ccgggtctgg
    20341 gccagcgatg acctggtaga ttagggccac atcctccaca gacgcgccca gcgggccgat
    20401 atgtgcgacg ctccagcata gcggcgcggc gccactctcg ctcaccctgc caaaagtggg
    20461 tttcagtccc accacaccgt taaggctggc cggcacacgt atcgaaccgc cgccgtcagc
    20521 gcccagcgcg gctggcaaaa ttcccgctgc aaccgccgcg cccgaaccgc tggaggaacc
    20581 tcccggatca tgctgcaggt catacgggtt ggcgatgcgc ccgtggtgca cgttttctcc
    20641 gtttgtggca atgccaattt catgcatatt ggttttaccc accaatacag caccggccgc
    20701 gcgcaggcgc gcggtcacag ttgcgtcttt cgcggcaggc cgtgcgccga ggaattgggt
    20761 gcccacagtg gtgggataag gcacctggtc cagttcgtct ttgatcgcca gcggcacgcc
    20821 ctccagcagg ctgcgcgcct gaccgttctt aagcctttcg gttgaatccc gcgcctgctg
    20881 gcggatatct tcagctgaac tggcaatgaa agcattcagc ggcggggtgg tctgctggct
    20941 ggcagcaata gacgcaatca cctcctccgc cacctgcgca ggggttacca cgccctccag
    21001 gtaggccgct gtgaaatcgc gaatcatatt gaacggcaac cgttcacgag aggggcggct
    21061 cagcgagggg atgtgctgag gcagttccaa gggttcccaa gcgcgctccg agggaataac
    21121 cagcgggaaa ttggttggct cttcgatgaa tttcatctcg cgtaatttgg ggattccacc
    21181 attctcaagc aggctgccaa ccaacagcga gcgggtcaat ttgttgctgg tgacggcgga
    21241 aaaaaacttc aacacgccgc catatagttt gggcaggttc agtgacttaa ggtcgtacat
    21301 ggtcattttt ctcccttttt ggcttctttt ttaagaaaac aggcaactgc gcgttggata
    21361 cctcgtttat gataaaccat tatatacgca aagcaggtga actgcccaaa gcactggact
    21421 acctcacctc acctagccgc cccgcccgcc tcgtaacctc aatcatcccc tcgggtggga
    21481 gtggtttttt cgtgagaggt ccttgcgctt ataccgaaaa aacctcgtta aacgggcaaa
    21541 agagtctcag agaaacgccg cctgaaagcc tgcttacggc ataacccggg tttgacctcg
    21601 ttaaaaaggg tttttctcag gttatttgac agccgcgcgc agggttatgt tttcctggcg
    21661 cgagtccaga aaaatcccca ataaagcgaa atggaaagca tatacaggat catcgtgagc
    21721 ataaatgccg gcgcaaagcc gtgcttaacc tggatataac cgctgatagt ggggctaaaa
    21781 gcccaaccaa aattgctggc catgctcacc aggctggcga ccgtggcgcg cgccgagggc
    21841 tcgacttttt ccaggacaaa ggtctggtac accggtccgc tcatattcat taatgccaca
    21901 cgaatataat aggcggcagc agcggcccaa tacagcgggg aaaaacccag caaagccagg
    21961 aacgggattg agagcgcttg agtaatgacc acaaactgga tctttccata tttctccgcc
    22021 agggggggcg cgatgagcag ccccacgccc atggcgagcg acccgaaagc aaacaacgta
    22081 ccgatggcgc tgtccgattg accgtgcacc tggcggaaga aaacgttcat aaatggcatg
    22141 accagaccag cgccaatgga tgtcaccagc atcggcagaa ccagtttgcc caacaacgcc
    22201 ggatggctct taaagtaagt aaagggggca aacagcgaac gttccaccac tttgccgcgc
    22261 tggatggtca tcaaaaaaag cggcaccacc gcggtgctgg cagccacagc cacaatggta
    22321 agtgcaagcc ggtaagcgct ggcgctggtg gcgtctcctc cggcgtatac tgccagccag
    22381 gtcggcagta acccaccaat ccagttgccc actgaagagg cggccattga aataccggaa
    22441 ctgaagctaa aaaggtaggt gcgctctttt tcaccgctgt tttccatcag gaatgggccc
    22501 atcgtcacac cggagatgct ctggcccagc cccatcaaaa tattcattaa tatgaagata
    22561 gtgggtgacg ggaataaaag cataccgcag atggcaacca ccattaccaa cccgccacca
    22621 atcaacgaaa gcttgcgccc cagcaggtca gccaggtaac ccatggggat ggccgcgacg
    22681 agggcggtca tactgctggc ggccaccagg ttgccgacca gcgcctggtc ataacccagg
    22741 ctgagaacgt aaaaattgaa caacaaacgg aacacaccga acgccgatga gctgaggatg
    22801 gtgcttaaca ggtagagtgt tgcattgcgc gagaatgcgc gcacattgcc agcgtaagct
    22861 cgcaaacctt gaagcacacg ttgatagaac agagtcgctg gtggcataat tttctgcggt
    22921 gatcgatgaa gctgttcttt aaggaaaaag ccggtttgaa gtttaccata attgttcatt
    22981 accgcttaca cccgctgccg ctggtgattt gcagtggaca tcagtctctt aaaagccaaa
    23041 gtcggattac aataagctac cgaaaacgct cacaggagaa aagacgatgc ttgatcaaat
    23101 aaaacagcag atcagccggg tcaaggcgtt gggagcgagt tacgtggaca cacgctggta
    23161 cccggtggaa gaaaccaatt cattgttaat gtggaacggc aatttgaaag aatcgagcgc
    23221 tacatttgaa agcgggctgg gcgtgcgcgt gctgtatcac ggcgcctggg gtttttcagc
    23281 ctcctcagat accagtgacc tcgcagcctt gtatgacagg gctcttgata acgcgcggat
    23341 tgcctccgag agactttctc tgccagtgag attggcggag aaagacgcca tccaggcgaa
    23401 atttgtgagc cccaacaaga taaacccgtt tgaagtccca ttatctgaaa aggtcgcctt
    23461 cttgagcgaa atggacaaaa aactggatca acccggtgta acccaacggg cgagcagcct
    23521 gacctttata cgcaaacaaa tcatcttcgt cgactctgaa ggcagcgaaa tcgagaaggt
    23581 cattaccgaa gtcttcgccg ggctcgaggt ggatggtgtt gatagcgagg gcgggatgta
    23641 tgaacgcaag ttcagcccct tccggcgcgg agacagccgg ggctgggaga ccattgacct
    23701 gcagcacttc agcgaaaacg cggagcgcat tgtacgcgaa ctcaacgaca cgctcaacgc
    23761 cgatccgtgc ccggttgacg accgctcggt cattctcctg cccggtatca tgtacctgca
    23821 aacccacgaa accatcggtc acgcgctgga actggatcgt attttgggat atgagctggc
    23881 tttcgccggc gggtcgttcg tcaccctgga tgattttggt aaattgcggt acggctctga
    23941 aaaattgacc gccagggcgg acgccacgct ccccaacacg ccaggaagtt ttggttttga
    24001 cgacgacggc attcccgcgc aagacaatct gctcattgac cggggtttgc tggttggcgc
    24061 gatcagcggg cgccagatgg tagaagaagc caacgcccgg gcacgccggc aaatttttca
    24121 tggcagcagc ggggcaaacc gcgccacctc cttttaccgg gtgccgatcg aacgcatgac
    24181 caatatcaat atcgaccctg gcaacgacgg cacgctggaa gaaatcatcc gcaatacaga
    24241 aaagggaatc gtgctggatg gagaccgcag ctggtccatt ggttccaacc gagagcaatt
    24301 ccactttgga acggaagcag gatggctggt agaggatggt cgggttaccc gggcggtcaa
    24361 gaacgcgacc tacaaaggcg ccaccctccc cttctggaat tcactcaccg cagtgggtga
    24421 cgcctccacc tgggaagttt attacgtgcc taactgcggt aaaggacaac ccaaccagat
    24481 catgcagttg ggtcatggcg tgccagtgtg ccgttttgac aacgtcagga ttggagaata
    24541 acaatgaagg ataggattct caatacatta gaccagttgc gccggtacgc gctttcaaag
    24601 ggctacagcg tctccatcta ttttcaagaa gaagacagct cgctgatgcg cttcgccaac
    24661 tcggccattt cgctgaatac caatgaacac ctgatccgcc tgaatatcac cgcttgtgat
    24721 ggtcgaaaac gcgccagtta cgggatgatc accgaccttt ccagggtcga agatatgcaa
    24781 agaggagtag agaccgcggc agaaatggtg aaacacagca tgcctctcac ctatgaatca
    24841 acgatccccg tcttccgaga agcgtttatc gacgagagcg gttacgaccc ctccttggct
    24901 gagatgagca acgctgagaa actggctttc ttcaaccgcg tcgtcgcgga catggaaagt
    24961 gacgaaatca aactttccgg agtcttttct aacggcgcga acactaccgc catggtcagc
    25021 accgccactc cgtaccccct gtatatcaaa acctccgacg cgcaggtgag cattgtattg
    25081 gctcaccaga ccctcaaatg ggaaattcaa tcggaacaat ccgcgcagaa gaaggatgag
    25141 ctctctgcgg atgccatgcg ccaggagctg ttgtgcctga tgtcacacta catgcacgac
    25201 actccccagc agctcccatt gggggtttac gatgttgttt tcgggcgcgc cgccatcgcg
    25261 gagatgctgc aattcatgag ctacatcggc atccacggcg ggttaatgaa gcgcggctat
    25321 tcctgcctga gcaaagaaga tatcgggaaa aagattttct ctgaacaatt cacccttatc
    25381 gatgacccca cccgcctgga aaccttcccg tttcgccgcg acctgagtgg aatgacacgc
    25441 cagcccttca ccctggtgga aaacggtata ttcaaaggct tcatctggat gcaggatgac
    25501 gcggatgaat ttggcgagca acccaccggg cacacgatcc cccatttgag cctgagcatg
    25561 gcaggcggct ctcaagcggt gggctctttg gaagaccttt ttaaccagcc gcgcgatcac
    25621 gacctgcttt acatcccctt cctgcattac atgaatatcg tcaacccgtc caaaggcatc
    25681 ctcaccagca gttcacgctt tggcgcggtc ttgttgaaaa aagatgggca gaccattatt
    25741 ccatacaatg tccgtctcac tcagagcctg ttggacattt ttggagacaa agtggattgg
    25801 ctatcagccg aaacgagcgc gtacaacctc tcaacgagct atgatgcgcg caaccccacc
    25861 gctttcatcg ttccgcggtt cgccagggtc aacgggctgg aaatttcaca ctccaactca
    25921 tcatactaaa tttacacgca gagcagcgag agcgcacaac cctctcgctg c
//
LOCUS       DAOTLW010000029        25736 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_2246_length_25736_cov_10.069273, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000029 DAOTLW010000000
VERSION     DAOTLW010000029.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 25736)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 25736)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25736
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2246_length_25736_cov_10.069273"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(84..1259)
                     /locus_tag="P5516_05915"
     CDS             complement(84..1259)
                     /locus_tag="P5516_05915"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HRT92009.1"
                     /translation="MFEKKTIRDIDIKGKKVLVRVDFNVPLNETGVGDDTRIRAALPT
                     IQYLLDGGAAVILCSHLGRPKGGPDPKYSLRPVAEHLSSLIGKPVKFSSDCIGPEAKE
                     AAEELKPGEVLMLENTRFHAEEEKNDAEMARQLASLADLYVNDAFGTAHRAHASTEGV
                     THFLPAAAGFLLEKEIKYLGQVIANPQRPFVAIMGGAKISDKIGVIKNLLTRADAILI
                     GGGMANTFMKAQGLAVADSLVEDDSLGIARALFEEAGGKIHLPVDMVVANQYAADAES
                     KTLKVQDVPDGWRILDIGPETVKAFRAIIATARTVVWNGPMGVFEFPKFAEGTFGVAK
                     AVADSAAVSVIGGGESVAAIQQSGLADKITHISTGGGASLEMLEGLELPGVAALLDK"
     gene            complement(1362..2414)
                     /gene="gap"
                     /locus_tag="P5516_05920"
     CDS             complement(1362..2414)
                     /gene="gap"
                     /locus_tag="P5516_05920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HRT92010.1"
                     /translation="MTTKVGINGFGRIGRQVLKAINEYHPGEVEVVAVNDLFDTETNA
                     HLFKYDSNYGILPVHIEAKENELIVDGKRIQVFAEKDPGMLPWKDLGVDIVVESTGVF
                     TDAKGDPAKDKPGAVVHIEKGGAKKVIITAPAKNEDLTLVLGVNDGWYDPAKHHVISN
                     ASCTTNCLAPAAKVVHEKFGILYGMMTTIHSYTNDQVILDQGHKKELRRSRAAGLNII
                     PTTTGAAKALALVIPELKGKFDGFSLRVPTPTVSIVDFVATIEKTITKDDLNAAFIEA
                     ANGSLKGILGYTTGEYGDPLVSMDFKGDPRSSIVDLPTNMVMGGNLVKVVAWYDNEWG
                     YSCRTADLAAMLGKKL"
     gene            complement(2476..3828)
                     /locus_tag="P5516_05925"
     CDS             complement(2476..3828)
                     /locus_tag="P5516_05925"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HRT92011.1"
                     /translation="MRRRRSKKFGRFIQSVLMQLRWLVPGIGIKRWVLVILAGTTLLG
                     LGLAVYLLDVYRTAPEGWWLPFIKIVALQFMPNRILRAFIFGGLGLVLIGIGITGLNR
                     ALLRPFLRPGKDVIDTVAEFRRKEKGPRIVVIGGGTGLTSILRGLKSYSSNITAIVTV
                     ADDGGSSGEIRKSIGILPPGDLRNCLAALSNDEEMMTQLFQYRFASGAGLNGHSLGNL
                     LITALTEITGSFEAAISESARVLAVQGSVLPATLKNINLVGEVELAENKTIIQVRGES
                     EISQAGGRVRRVWLDPGNPTSYPPTIQALLNADLILVGPGSLYTSIIANLLVPDLLEA
                     FKASKAYKFYICNIATQRGETDGYTCEDHVRAIEKHAGHRLFDVVLANNRYDHHMPEG
                     TEWVKVCEDDNRYPYYRADFVNVEKPWRHDPSKVAKTIIDLYMERTGPLVSKEEKSSI
                     "
     gene            4055..5365
                     /locus_tag="P5516_05930"
     CDS             4055..5365
                     /locus_tag="P5516_05930"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HRT92012.1"
                     /translation="MNPTRLPGQKALEIIQRDQQVISPSYPRGYPFVMDHGKGVYVWD
                     VDGNRFLDFAAGIAVNSTGHSHPDVVKAIQDQAEKFIHISSDFYHAKWVELAEYLNRI
                     APFKEDAVIFQTNSGTESVESALKLARYHTGRSQFIAFMGGFHGRTYGAVTLTASKAK
                     YHHGFFPLMNGVTHVPFPDPYRPMLHMLPGEDIGDATIRFIEKVFIHNLPPQDVAGII
                     IEPIQGEGGYVVPPDTFISQLRELCSRHGILLIADEVQSGVGRTGRWWAIENFGVEPD
                     IVTTAKGIASGMPLGAMIARKSITTWPKGAHGNTYGGNPLACVASLETLQLIENGYMQ
                     NAKHVGSYCLDRLAEMQVRHPSIGAVRGMGLMIGIEFVMDRESREPAEKMRDAVVDKA
                     FELGLLTLGCGTSTIRISPPLCITREEIDEGLTILDEAITFAEK"
     gene            5410..6969
                     /locus_tag="P5516_05935"
     CDS             5410..6969
                     /locus_tag="P5516_05935"
                     /inference="COORDINATES: protein motif:HMM:NF013736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT92013.1"
                     /translation="MLPDFRVRQRDYLLEISRALTQELDLVKLLDRILHISLEMLAGQ
                     AGLIALRVPSEGWQIQVSTGLPPAFLRFLEPQLAQISEEHDSSDLELKTINHILGNFT
                     QSISLGRLSSVGLPLITGQKVLGAIFVFREFAAFSANDRALLASFADQAAIAVQNAQL
                     YTQVENDRRRVTGLLDSAADGILILTRALRVERVNPSLAHLVGIQPQDLTGKEHDEVI
                     RWVKPPHGMTLTQAIADGWPLTPHAALYVEGDLERTGGTRPVPVGITYAPLLSEEGKL
                     VNIITTVRDITRFREADELKATFISVVSHELKTPVALIKGYVSTLRRDDAHWEKAVIN
                     DSLQIIEEEADRLSEYIENLLDVTRLQANGFNLKRNDTQLAEIIKRVAERLQTQTIKH
                     TIIVEIPQPLPIIVADDSRINQVISNLVANAIKYAPGGEIRIKAVQRESNIIICVSDE
                     GPGINPNDVPFIFDRFYRGSDASRLTKGAGLGLFLTKSIVEAHGGRIWIDSKIGQGTR
                     ICFCLPIASTG"
     gene            7057..8409
                     /locus_tag="P5516_05940"
     CDS             7057..8409
                     /locus_tag="P5516_05940"
                     /inference="COORDINATES: protein motif:HMM:NF025711.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HRT92014.1"
                     /translation="MPKIQTACPRCKQPITAEVQMLFDMNTDPTAKQKLLGRANNFAH
                     CQACGYEGLISTPIVYHDPNKELLLTYFPGELGFPLNEQEKQIGPLVNQVVNALPAEK
                     RKGYLFQPNSMFTYQSLIDRILAADGITKEMIEAQQKRVNLIERLLSLPNEEDRVTVV
                     RQEANLVDGETFTLLSTLIQSASAQGDEASAKLLSQIQAVLLNETDFGKKLLADSQET
                     QHALKELDKAGKEGLTREKLLEVLSSLKSETSLTTLVSLTRSGLDYQFFQILSEKIEQ
                     STDENKQALISLRDKLLDLTRKIDNEVKKRLENAASLLENILKEADVQAAIEKHAEEI
                     DEFFTQAVQVEFEKAHSENNLERMEKIQKVVSILDKMTAPPPEVVLIQKLLDASDDET
                     RKKILQENETLVNDQLLQTINAIITESSTKSQAPELIDTLKSIYKMALRAHMEKNLKS
                     "
     gene            complement(8502..9179)
                     /locus_tag="P5516_05945"
     CDS             complement(8502..9179)
                     /locus_tag="P5516_05945"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HRT92015.1"
                     /translation="MRNSSFGNSITHEFPVLVALQGPLEGRRWIIREAIDIGRDEDCA
                     IRIEERQVSRHHARLRNENGICYIEDLGSKNGTFLSGAPLIESTRLGDGDTFQIALIQ
                     KFAFFSSDATMPIEDLDPTFLKGSGKLVLEERSRRVWIGREELLPPLSAAQFRLLATL
                     YNQAGEVVTREALIRKTWEEDEATGVSDQALDALIRRLRERLAELDQKHQYIVTVRGH
                     GLRLENP"
     gene            9275..10258
                     /locus_tag="P5516_05950"
     CDS             9275..10258
                     /locus_tag="P5516_05950"
                     /EC_number="2.7.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine-protein kinase"
                     /protein_id="HRT92016.1"
                     /translation="MISPLKKGNILRERYTIRRVIGQGGMGCIYLADDLRLEGRQCAV
                     KEVEHDRTLPKNLLKEARDQFQREATVLARLDHPNLPKVSDYFSIENSDYLVMDFVPG
                     EDLRTLMLKQREKNQFIPEKDVLNWAFQLADALEYMHNQNPPILHRDIKPSNLKVTPA
                     GLLKLVDFGLVKVLAPGEVTITILQGQGTALYTPLEQYGGDIGHTDARSDIYAFAATL
                     YHLLTAKPPVDARQRFISPNALQPIRETNQAVTQKTENAILWGMALHPDDRPSTIGQF
                     REALIGSRPVAIPSGNRLSVKIPTFYNRKIGKQWYILMGLSLLTLLVTLLR"
     gene            complement(10262..13003)
                     /locus_tag="P5516_05955"
     CDS             complement(10262..13003)
                     /locus_tag="P5516_05955"
                     /inference="COORDINATES: protein motif:HMM:NF013126.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HRT92017.1"
                     /translation="MQKIFLLFLALSLAAPTATAQAGDRAQTNARLAAAQVRLAQMTT
                     EEKVGQVFLISFQGSSAQEGSRIVRLIQEKHIGGVVLRKDNDNFIGPENTIGVLKELV
                     TDLQAAAQMVENQESYAPLLVAITQNGYLDVDGQILHGVTNLPNLMTIGASWKTEQAS
                     AVGSVLGSELEALGINMLIGPSLDVLDIIHTEGRDDLGVKTFGGNPYWVGKLGKAYIE
                     GVQAGSANRVAVIATHFPGSGSSDRQPDEEIATVRKSLDQLMRVELAPFFNVTKIDQG
                     HIGVVDGLLLSHIRYQGFQGNIRSSTKPISFDPVAVEQIMNLPELSPWRKMGGLIVSD
                     DLGSESIRKFVDPLNTGFDARQVARSALMAGNDLLYMGNILATGDEDNYSTVVRTIDY
                     FVQKYEEDSAFHNRVDEAVLKILQLKMKLYPSFDPADVMTPRNQLETVGKAQATVMEI
                     AQSAVTLISPEMEDLNTVLPDPPGANDRIVFISNVQTNTQCSSCPVETDFPAARLMNS
                     IIRLYGSGAGDPIPASHLTAYSYENLRMVLEQADRTEDILVSLNAATWIIFSFAEFGA
                     ERVEATTYKRLFDERPDLVRNKKLIGLAFNAPYFLDSTDISKLTAYYALYSNTPEFYE
                     IASRVVMQELTPSGKLPVSVPGIGYDLVYVLSPDPTQMIPLVIETPVEIDEPQAPPLA
                     GYSQPLLYKAGDTIPIKAGVIVDHNGNPVPDGTLVRFIIDTRSTSGSVEQLEARTIDG
                     FARVMYVIPSIGSLQLSVTADPAFISQILRLDITDTGGVVTSFEPTPIPVSSDIATPT
                     EASPSPTPESKVIQLHKQGKVAFWDWLLANILIVGFCLGLSYFTHNHLSAKWNFLTIL
                     FSGAGGYAGYLWAALGLPGSKSPLNEVASGQILILVGIGLVCGFGSGFLFYWWKTRK"
     gene            complement(13031..13975)
                     /locus_tag="P5516_05960"
     CDS             complement(13031..13975)
                     /locus_tag="P5516_05960"
                     /inference="COORDINATES: protein motif:HMM:NF012512.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRT92018.1"
                     /translation="MITAEWVIDAAKRIQPIIEQTPVTKVPQLDILCKWENYQKTGSF
                     KLRGASNKVLSLEKSALKGGLVACSAGNHGQGVAFAAASRGSACMVFVPENTSASKID
                     AMRQFGADVRLVRGGYVEAEKQALAHAQTSGKTFISPYNDAQVIAGQGTIGPELVEQL
                     GGLDGIRSLVVPVGGGGLISGIGVYLSNMKERPQLIGVQSETSPYAHQVYYSGTQTGV
                     TETESIAEGLAGEIAHDSITIPLMRRFVDDIVLVSEEEIRLAIRYCWKKYGVRIEGSA
                     AVGLAARLAGKIQLFPAALILTGGNIQSELFNEIIHSK"
     gene            complement(13972..14718)
                     /locus_tag="P5516_05965"
     CDS             complement(13972..14718)
                     /locus_tag="P5516_05965"
                     /inference="COORDINATES: protein motif:HMM:NF015000.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HRT92019.1"
                     /translation="MLDDLPKPVVFAHRGASQYAPENTLAAFELAVSQGADAIELDVK
                     LSADGKAVVIHDQTVDRTTDGSGRVNQMTLASLKGLNAQYRFDAYAGEQIPTLDEVFE
                     SVGKRIFINVELTNYASRHDNLVPIVAEIVKRHQLEKEVLFSSFIPGNLKKMKILLPD
                     VPVALLASQGLLGVASRSALFIQLSPKIIHPYLDDVTPQLMNTERRRGRRVHVWTVNE
                     DNDLRRMIDYGVDGFFTDDPAKALRILERK"
     gene            complement(14719..15897)
                     /locus_tag="P5516_05970"
     CDS             complement(14719..15897)
                     /locus_tag="P5516_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT92020.1"
                     /translation="MTGYKVAEALCLPFTGMVDQLLDALKRDKLVEVKSSQMGLGEGA
                     YLYAITGAGITHAREALDRCQYAGPAPVPLEAYNESVLRQSRGRMFVTSRNMRQIMGD
                     LVFGDEVFHRLGPAVNSGTSIFIYGAPGNGKTSVARAIGNQVLTQTMYIPYAIFVDGQ
                     VITMFDSMNHQLAAEDETGPQNRGMKGRKDQRWVKIQRPFIMVGGELTLTGLDLVFNE
                     TLKFYEAPFQVKANGGILLIDDFGRQQVRPRDLLNRWIVPLENRIDYLSLHTGRKIEV
                     PFDVLVMFSTNLPPKDLVDEAFLRRLRHKIEITDPDAVHYREIFMRVAAQKGVVFSDE
                     GLNYLLKEWYAKHNRKLRASHPRDLCDQIIDISRYFSIEPVMSREMIDQAASSYFVEL
                     "
     gene            complement(16129..17046)
                     /locus_tag="P5516_05975"
     CDS             complement(16129..17046)
                     /locus_tag="P5516_05975"
                     /inference="COORDINATES: protein motif:HMM:NF013088.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HRT92021.1"
                     /translation="MEKTPSRIKAILLNFLVVIIWASSWVLIKIGLKDIPALTFAGLR
                     YFLAFLFLLPFIFQKKVRQQIRDFNKSDWRLIILMGVFSYFVAQGTQFLSLDYLPATT
                     LSLLLNLTSILVTISAIFLIKELPTKVQVLGVLINLAGMFVFFYPDGFSGNSTLGFVF
                     GVICLLANAIGTVVTRKINSGGRVNPIAVTLTAMGIGSIPMLVAGILWQGLPHLGLQS
                     WVIILVLALVNTAFTFVVWNYTLQTLNAMESAIINGTMLVFVAIFAWIFLKETQDLAG
                     IIGLALAFLGAVIVNIRFERKSTSQPKVN"
     gene            complement(17048..18760)
                     /gene="recJ"
                     /locus_tag="P5516_05980"
     CDS             complement(17048..18760)
                     /gene="recJ"
                     /locus_tag="P5516_05980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008297 - single-stranded DNA
                     exodeoxyribonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HRT92022.1"
                     /translation="MAERNLTKRWEIAPKIDGMADEALADYPPFFRQLLYNRGIRTAQ
                     GAQQYLSNDGELADPFLLLGMESAVDRLVAAIDNGEKIVVYGDYDVDGVTATVMMVEV
                     LQRLGGNVEPYIPNRFEEGYGLNDEAIQTLASEHSAKVILTVDCGIRSPREAITASNL
                     GIDLIISDHHFPKGDLPAGYAVLCAKQERDPYPYKDLAGVGVAYKIAQALFIKKGMDV
                     GKADEWLDLVALGTVADMLPLTGENRIMVARGLNHLRNGSRVGLNALIGVSGRNVQQV
                     TATDIGFILGPRLNAAGRMESALQAYELLVSKSAVDAVKAAQVLDNQNSERQKATLAA
                     QEKAQGAVTHLESTYLITAFDSEFSSGIVGLVASKLVEKFHRPAIVGERLGDTIRASC
                     RSINGFHITKALDECADLLVRHGGHELAAGFTVKMENEDQLIARLQEIAARELSAQDL
                     RPTLRADMEIELSRFKPEYYRMIDQLQPTGMGNPAPLFISRGVVVDRMQVMGKESTHL
                     SFSVRGSQINRAVAFNQAAWYQTWYEEKPRFDLAYTVEVNHYNGMDTLQLNIRDMKIS
                     AVSF"
     gene            18919..20715
                     /locus_tag="P5516_05985"
     CDS             18919..20715
                     /locus_tag="P5516_05985"
                     /inference="COORDINATES: protein motif:HMM:NF013132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HRT92023.1"
                     /translation="MTQDQTFTLIILLAAVILFASNRVRADMIALIVMVSLGLTGVTS
                     ADEAFSGFSSSAVMTILGISIISIALQITGATNSLGNILHTAGKGNEKLVILLVTLFG
                     ALVSLFMNNIAAVGVLLPAVMTLSRRSKISPSRLLLPLAYGTILGGMATLLTTSNIIV
                     SGALKDAGLPYFGLLDYFPIGGPVMLMGILYLSFIGFRLLPAKSPTNKIEDRREFTEK
                     LSDFYQVGKNLVKIELLPQSEMAGKTIANGHWYNRVHVNILAVLREKENIFSPDPCTM
                     LKPGDILIARGKVTEQSAKDLNLRVSAINNQEIKITDEAHPLAEVIISPHSRFIGKSL
                     QQMDFRERYQLNVVGVWRGGKPVEGEYADLSLHFGDALLVQGPSIQIRNLKKSEDLVV
                     LEEDPDAVLLPRKGLLTIFITLLTLTIAGLGFLPVALVVMGGAVLLILTGCVGLNDAF
                     RSIEWKAILLIAGLWPMSIALQSTGLAEMAVNSLLRLSGGLPPLQLAALFLFVAALLT
                     QLMSGQVTAIVMIPLALVSAQRMGIDPRPFAMAVAMGCSLAFISPLGHPVNIIVMNPG
                     GYTLKDYVKVGLPLTIAAFLCVIFAIKLYWGL"
     gene            20725..21846
                     /gene="tgt"
                     /locus_tag="P5516_05990"
     CDS             20725..21846
                     /gene="tgt"
                     /locus_tag="P5516_05990"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - queuine tRNA-ribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HRT92024.1"
                     /translation="MEQIDFSYTLLKEQGSARAGIFFTPHGPIETPVFAPVGTQATVK
                     AVTPAQLEQTGATLVLANTYHLYLRPGAELVAAHGGLHSFMQWQKPLLTDSGGFQVFS
                     LSGSRKVDEDGVTFKSHIDGSMHRFTPESSIQIQELLGADIIMAFDECAVPDDLLYSR
                     VALERTHAWLERCIRQKKRPDQALFGIVQGGVFAELRRESARFVSSFDLPGVAIGGLS
                     VGETKAQMHAALEEVTPLLPRTKPRYLMGVGSPEDLVNGILRGVDIFDCVLPTRLARH
                     QAAFTPNGRVNLMNRMFAHDFNPIDESCHCYTCQHFSRAYLRHLIVAKEMLSATLISI
                     HNIQFLVDLVKRARKAILEGSFPLFASSFLEAYQIKEPS"
     gene            21843..23423
                     /gene="uppP"
                     /locus_tag="P5516_05995"
     CDS             21843..23423
                     /gene="uppP"
                     /locus_tag="P5516_05995"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HRT92025.1"
                     /translation="MSLIQSLILGIIQGFTEFLPISSSAHLVLVPFLFNWAIPENQVF
                     PFDVLVQLGTLAAVILYFWKDLWKIIKAFVTGLIRRQPFAEEDARMGWYLLLATLPAG
                     FAGIFLKERVEAAFNNPRLTAIFLFVTAVFLVGAEFFGRRSRQLDKMTWKDALWVGVF
                     QAFSIFPGVSRSGSTIAGGMTRNFKRPAAARFAFLMSIPIMLAAGIFSLPDLFAVPDL
                     PAFLPIVLVGLIAAGVVGYLSIRWLLSFLVKRSLIIFAVYCILLGSAVLIISGIRQTS
                     PNVAGTAPGAPAAESAEYPPQTPISIQETLPLEISPALDWLRPRISGCADAIPGLALV
                     TEGTVPANGEPMQLSWGEPPNLKRKAVIIGEDDLVFIVNPRNPLRELPLALLQPLLSG
                     ELNSWGEVQVACPECFSAGQLDELIDQPVEVLLYPANVDARRVMDEALSSAPVRPNAS
                     SVLVPSAEMMKQEVSSALNAFGTLPSRAVDETVKVIPIQNGAETLKITRPLLATSAAE
                     AQPLTLQFLTCLQAAITP"
     assembly_gap    23535..23634
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(23657..24994)
                     /gene="glnA"
                     /locus_tag="P5516_06000"
     CDS             complement(23657..24994)
                     /gene="glnA"
                     /locus_tag="P5516_06000"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamate-ammonia ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HRT92026.1"
                     /translation="MDKKELLERVAEDRVRFISFQFSDVSGTVKSVDAPISRLEGAVN
                     QGIWFDGSSVEGFARIQESDMRLKPDIDTYAVLPWSPENMRRARLFCNIYNPDGTPFA
                     GDPRGNLSRQLAALEERGMRLMVGSEPEFFLFRRNGSSDIHPVPHDVGGYFDFSPNDE
                     AVRVRTELMQALDSMGLEVEVGHHECALGQHEIDFHFSNALRSADNVLTLKYTVKAIA
                     AQNGLIASFMPKPIFGINGSGMHCHQSLFDLRGNNLFYDAADEYHLSPLAYGFIAGQL
                     AHARAFSAVTAPTVNSYKRLVPGYEAPVYVGWAQTNRSALIRIPRPLEGMTTSVRAEL
                     RCPDPSCNPYLAYSSMLAAALDGMKQQLTPPEPLNDVNVYELSREERQNLNIVELPGS
                     LREALVELEKDSVIQQALGETLYNAFRRAKWAEWERYRLDVTDWELARYLETA"
BASE COUNT         6144 a         7090 c         6205 g         6197 t
ORIGIN      
        1 atcatttgaa ccaacgatgc accttcggta acattttctt ttggattgac agggataatg
       61 gagcgggttg agagttgaga gatttactta tccaacaggg cagccacacc cggcagttcc
      121 agcccttcca gcatctccag cgatgctcca cctccggtgg agatgtgcgt gatcttgtct
      181 gccaacccag attgttggat ggcagccact gattctcccc cgcctatgac gctcaccgca
      241 gcgctatccg caactgcctt cgccactcca aaggtacctt ccgcgaactt cggaaattca
      301 aaaacgccca tcgggccgtt ccaaacgaca gttcgcgctg tggcaatgat agcccgaaaa
      361 gccttaaccg tctcggggcc aatatccagt atgcgccagc catccggaac atcctggact
      421 ttcaacgttt tgctttcagc atccgcggca tattgattcg ccaccaccat gtctaccggt
      481 aggtgaattt ttccgccggc ctcttcgaat aaagcacggg caattcctaa cgagtcatct
      541 tccaccagcg aatcggccac agccaacccc tgcgctttca taaaagtatt cgccattcct
      601 ccaccaataa gaatggcgtc tgccctggtc agcagattct tgatgacgcc gatcttatcg
      661 ctgatcttcg cgccgcccat gatcgccaca aagggccgct gcgggttggc aatcacctga
      721 ccgagatatt taatttcctt ctccagtaag aatccagccg ccgctggtaa gaaatgcgtg
      781 actccctcag tagaagcatg cgcacggtgc gctgtgccga atgcatcgtt cacgtaaagg
      841 tccgccaggc tggccaattg cctggccatt tcagcatcat tcttctcttc ttcagcgtgg
      901 aagcgtgtgt tctccagcat caacacctcc ccgggtttca gttcttcggc agcctccttg
      961 gcctccgggc caatgcaatc tgaggaaaac tttactggtt tccctattaa cgaagaaaga
     1021 tgctctgcca cgggtcttaa tgagtacttc gggtccggcc ctcctttggg tcgtcccagg
     1081 tgcgagcaca aaatcacagc cgctccccca tctaaaaggt actggatagt gggcagcgcg
     1141 gctctgatgc gggtatcatc accaacacca gtctcattaa gaggaacatt aaaatccact
     1201 cttaccagaa ctttcttccc ttttatgtca atatcccgaa ttgtcttttt ttcaaacatc
     1261 gttcattctc ctggttaagg taagatgcgc aatccgttgg tttttcggtt gcgcatcgta
     1321 ttcatcaagc ttacccggga aacatcaccg gatgacttct tctataactt ttttcccaac
     1381 atcgccgcca ggtcagcggt acggcaggag tagccccatt cgttatcgta ccaggcgacc
     1441 accttgacca aattaccgcc catgaccatg ttcgtaggca ggtccacgat cgaagagcgc
     1501 ggatcccctt tgaaatccat cgacacgaga gggtctccat actcgccagt ggtgtacccg
     1561 aggattcctt tcaaagaacc gttggctgct tcaatgaaag cagcgttcag atcatctttc
     1621 gtgatcgttt tttcaatggt cgcaacaaaa tcaacaatgg aaacggtcgg cgtcggaacg
     1681 cgcagcgaga agccatcaaa ttttcctttg agctccggaa tcaccagtgc caaagccttg
     1741 gcagcgcctg tggtggtggg aatgatattc aagccagccg cgcgagagcg ccgcagttct
     1801 tttttatgtc cttgatcaag aatgacctgg tcattggtgt aagaatggat tgtagtcatc
     1861 atgccataaa ggatgccgaa cttttcgtga accaccttcg cggctggcgc caggcagttg
     1921 gtggtgcaag acgcattgga gatcacgtgg tgcttcgcgg ggtcatacca gccatcattc
     1981 acaccaagta ccaatgtgag gtcttcattc ttggcgggtg cggtgataat caccttcttc
     2041 gcaccgcctt tctcgatgtg gaccactgcg ccaggtttgt ccttggctgg gtcgcccttg
     2101 gcgtcggtaa acacgcccgt agattccacg acaatatcca ctcccaggtc tttccaaggc
     2161 aacattccag ggtctttttc cgcaaaaacc tgaatcctct ttccatccac gattaattcg
     2221 ttttctttcg cctcgatatg cacaggcagg ataccgtaat ttgaatcgta tttgaataaa
     2281 tgggcgtttg tttctgtatc aaacaaatca ttgacggcca ccacttccac ttccccgggg
     2341 tggtactcat tgatcgcttt gagaacctga cggccgatac ggccaaaacc attaatacca
     2401 actttggttg tcatctactt cctccaaaat ttaaaatatc gatgaatttt accatgtcca
     2461 gacaagatta aataattaaa tggatgattt ttcttcttta gagacaagcg gcccggtacg
     2521 ttccatatag agatcaatga tggttttcgc cactttcgaa gggtcgtgcc gccacggttt
     2581 ttcgacattc acaaagtcag ctcggtagta aggatagcgg ttgtcatcct cacaaacctt
     2641 aacccactcc gtcccttctg gcatatgatg gtcataccgg ttgttagcaa gcactacgtc
     2701 aaatagcctg tgacccgcgt gtttttcaat cgctcgcacg tgatcctcgc aggtataccc
     2761 gtctgtttca cctcgctgtg tggcgatatt gcaaatatag aacttgtagg ctttgctcgc
     2821 tttgaatgct tccagaaggt caggaaccaa cagattggcg ataatactgg tgtaaaggct
     2881 cccggggcca accaggatga ggtcagcgtt tagcaacgcc tgaattgtcg gcgggtagga
     2941 tgtcgggttg cctggatcca gccaaacccg gcgcacccgc ccgccagcct ggctgatctc
     3001 gctttctccc ctcacctgga taatggtttt attttccgcc agttcaactt caccaacaag
     3061 attgatattt ttaagagttg ccggcaagac acttccctgc actgccagca cccgggcgga
     3121 ttccgagatg gccgcttcaa aacttccagt gatttctgtt aatgccgtaa tcagcagatt
     3181 gcccaatgaa tgaccgttta agcccgctcc ggaggcaaat cgatattgaa aaagctgggt
     3241 catcatctct tcgtcgttag ataaggccgc aaggcaatta cgcaagtctc cgggaggtaa
     3301 aataccgatg gatttacgaa tttctcctga cgaaccgcca tcatccgcca cggtaacaat
     3361 cgcggtgatg ttgctggagt agcttttcaa tcccctcaaa atggacgtca accccgtgcc
     3421 gccgccaatg accacaattc tcgggccttt ttctttgcgt ctgaactcag ctacagtgtc
     3481 gatgacatcc ttgccggggc gtaagaaggg ccgcaacagg gcgcggttta atcccgttat
     3541 accaatgccg ataagcacca gaccaagccc gccaaaaatg aacgcgcgca ggatacggtt
     3601 gggcatgaat tgtagtgcaa caatcttgat gaatggcagc caccaaccct caggcgctgt
     3661 gcggtaaaca tccagcaggt acaccgccaa gcccaacccc agcaatgtcg tgccggcgag
     3721 aatcaccagt acccagcgct tgatgccaat acctgggacc agccaacgca actgcattag
     3781 cacagattga ataaatcgac caaatttctt cgagcgtcgt ctacgcatta tgctagataa
     3841 tagcagggtt taagaggtgc gtcaataaat ttcttggcag cggttgtgag agacaaaagg
     3901 ctgtttttcc acttaccatg gttgccacca tgaacgataa cctagataaa aactgcaatt
     3961 tgagcgctca tgttataatc tctttaaggc cgaatgcaag gcgcaataaa tccgcagtag
     4021 tacgaataaa attcaataac cccaggaggg cggtgtgaac cccacaagat tacccggcca
     4081 gaaagcactc gagattattc agcgcgacca gcaggtgata tctccttctt acccgcgtgg
     4141 ttacccgttc gtgatggacc acgggaaagg cgtttatgtc tgggatgtgg atggcaatcg
     4201 gtttctggat tttgcagccg gtattgcggt caattctact ggccacagcc acccggacgt
     4261 ggtaaaagcc atccaggacc aggctgaaaa attcatccac atttcttcgg acttttatca
     4321 cgctaagtgg gtggaactgg cagagtatct caatcgtatt gcccctttta aagaagacgc
     4381 cgttattttc cagaccaatt caggcacaga aagcgtcgag tcagccttaa aactggcgcg
     4441 ttatcacacg ggacgcagcc aattcatcgc cttcatggga gggtttcatg gacgcacata
     4501 tggcgcggtc accctgacag ccagtaaagc caaataccac cacggctttt tcccgctcat
     4561 gaatggcgtc acgcatgtgc cattccctga tccctaccga ccgatgcttc atatgctgcc
     4621 gggtgaagat atcggcgatg cgaccatccg ttttattgag aaggttttca ttcataacct
     4681 ccctcctcag gacgtggctg ggataatcat tgaacccata cagggcgaag gtggttacgt
     4741 tgttccgcca gacactttca tctctcagtt acgtgaactg tgcagccggc atggcatcct
     4801 cctcattgcg gatgaagttc aatcaggtgt tgggcgtacc ggtcgatggt gggcgataga
     4861 aaactttggt gttgaaccag acattgtcac cactgccaaa ggaatcgctt ctggcatgcc
     4921 gctgggcgcg atgatcgcgc gcaagagcat tacaacctgg ccaaaaggcg cgcatgggaa
     4981 tacctacggc ggaaatcccc tggcttgtgt cgccagcctt gaaacactcc aactgatcga
     5041 aaacggctac atgcaaaacg ccaaacacgt gggcagctac tgccttgatc gacttgctga
     5101 aatgcaggtc aggcacccat ccataggggc tgtgcgcggc atgggcctca tgatcggcat
     5161 tgaatttgtg atggatcgtg aatctcgtga accggccgag aaaatgcgcg acgccgtggt
     5221 ggacaaagcc tttgaactcg gtctgctcac gttgggctgt ggcaccagca ccatccgcat
     5281 ctctcccccg ctgtgcatca cgcgtgaaga aatcgatgaa ggacttacta tccttgacga
     5341 agcgatcacc ttcgctgaaa agtaacacca aaaccaatcg gcttcattcg aaagcctgca
     5401 gaaaccgcca tgttacctga ttttcgagtc cgccagcggg attatctgtt agagatttca
     5461 agagctctca cccaggagct ggatctggtc aagctcctgg atcgcatatt gcatatttca
     5521 ttggagatgc tcgccggtca ggcggggctc atcgcgctgc gtgttccttc agaaggatgg
     5581 cagatacagg tctccaccgg ccttccgccc gcatttttaa ggtttcttga acctcaactg
     5641 gctcaaattt ccgaggaaca tgattcatcc gatttggaat taaagaccat caaccacatc
     5701 cttggcaact tcacccagtc catcagcctg ggtcgattga gcagcgtggg tctgccactg
     5761 atcacgggtc aaaaagtgct gggggcgatc tttgtttttc gtgaatttgc ggcgttttca
     5821 gccaatgacc gggcgctgct ggccagcttt gcagatcagg cagccattgc cgtgcagaat
     5881 gcgcagttat acacccaggt tgagaatgac cgtcgccgtg ttaccgggtt gttggactcg
     5941 gccgctgatg gtatcctgat tctcaccaga gccctgcggg tggaaagggt aaacccttct
     6001 ctggcgcacc tggttggaat acagccccag gatttgaccg gtaaagagca tgatgaagtg
     6061 attcgttggg tcaaaccgcc gcatggaatg accctgactc aagccatcgc ggatggttgg
     6121 ccgctcaccc ctcatgccgc gctctatgtg gaaggcgatt tagaaagaac aggcggtacg
     6181 cgccctgttc cagtgggcat tacctacgcc cctctgcttt ctgaagaggg aaagctcgtg
     6241 aatatcatca ccaccgtccg ggatatcacc cgtttccgtg aagcagatga acttaaagca
     6301 actttcattt cagtggtaag ccatgagctt aaaacgcctg tcgcgcttat taaaggatat
     6361 gtcagcacgc tcaggcgcga tgatgcccac tgggaaaaag cggtgattaa tgacagcctg
     6421 cagattattg aagaagaagc agatcgtttg agtgaataca ttgaaaacct gttggatgtg
     6481 acccgcttgc aggcaaacgg attcaacctt aagcgcaacg acacccaact ggctgaaatt
     6541 atcaagcgcg tggcagagcg cctgcaaacc caaaccatca agcacaccat tattgtggag
     6601 attcctcagc ccctgcccat catcgttgca gatgattcac gcatcaacca ggtcatctcc
     6661 aacctggtgg caaacgcaat caaatatgcc cctgggggtg agattcgcat taaagcagtc
     6721 cagcgcgaat ccaatatcat catctgcgtc agcgatgaag ggccgggcat caaccccaat
     6781 gatgtgccgt ttatttttga ccgcttttac cgtggttcag atgccagccg tctaacgaaa
     6841 ggcgcagggt taggtttgtt tttaaccaaa tcgatagtcg aagcgcacgg gggaagaatt
     6901 tggatcgatt caaaaattgg tcagggtacg cgcatctgtt tttgcctgcc tattgccagc
     6961 accgggtaac cagcacaaaa caaccgtttt tcatcaaaaa ccgcagtggt ataatcaccc
     7021 tgttaaattg atctttttga atgaaagagt ggaactatgc caaaaattca aaccgcatgc
     7081 cctcgctgta aacagccaat caccgctgag gttcaaatgc tcttcgatat gaacacagac
     7141 cctaccgcga agcaaaaact acttggcaga gccaataact tcgctcactg ccaggcttgt
     7201 ggttacgaag gtctgatcag cacgccaatc gtctatcatg atcccaataa agagttgctg
     7261 ctcacttatt ttcctggtga gctcgggttt cccttgaatg aacaggagaa gcagattgga
     7321 cctttggtca accaggtagt aaatgcgctg ccagccgaaa aacgcaaagg ctatttattt
     7381 caacctaatt cgatgtttac ataccagtcg ctgattgatc gcattctggc ggcagacggc
     7441 attaccaaag aaatgatcga agcccagcag aaacgtgtta acctgattga acggttgttg
     7501 agtttgccca atgaagaaga ccgcgttacc gtggtcagac aggaagccaa cctcgtggac
     7561 ggcgagactt tcacactgct ttcaacgctg atccaatctg cctctgcgca gggcgatgaa
     7621 gccagcgcca aacttctcag ccagattcag gcagtgctgc tgaacgaaac cgatttcgga
     7681 aagaaactgc tcgctgacag ccaggaaact caacacgccc tcaaggaact ggataaagcg
     7741 ggcaaagaag ggctcacgcg cgaaaaactt ttagaggtgt tgtcgtcctt aaaatctgaa
     7801 accagcctaa caacattggt gagccttacc cgctccggct tggattacca gttcttccaa
     7861 atcctgagtg aaaaaatcga acagtcaaca gatgaaaata agcaggcgct catctcactc
     7921 agagacaagc ttctcgacct taccaggaag atcgacaatg aagttaaaaa gaggcttgaa
     7981 aatgccgctt ccttgcttga aaacatcctg aaagaagcag atgtccaggc agccattgaa
     8041 aaacacgcgg aagaaattga cgagttcttt acgcaggctg tccaggttga gtttgaaaaa
     8101 gcgcacagcg aaaacaacct cgaaagaatg gaaaaaatcc agaaagtcgt ctcgatattg
     8161 gataagatga ccgccccgcc gccagaagta gtgctcattc agaaactgtt agatgccagt
     8221 gatgacgaaa cccgcaaaaa aatcttgcaa gaaaatgaaa ctttggtgaa tgaccagctt
     8281 cttcaaacca tcaacgccat catcaccgaa agctcaacca aaagccaagc gccggaactt
     8341 atcgatacat taaaaagcat ctataagatg gctttacgtg cccatatgga aaagaacctg
     8401 aaatcctgat ttttccaacc ttaacaaaca actccccgag gctaccccgt ggctgccttg
     8461 gggagttgtt ttaccagtgc tgaatggtca agcaggagtc ttcagggatt ctccagcctg
     8521 aggccatgac cgcgtaccgt gacgatatat tgatgttttt ggtcgagttc tgccagccgc
     8581 tcgcgtaagc gccggatgag cgcatcaagc gcctggtcgg acacaccggt cgcttcatcc
     8641 tcttcccagg tttttcgaat caacgcttct cgtgtaacca cctcgcccgc ctggttatac
     8701 aacgtcgcga gaagacggaa ttgggcagcg gataaaggag gcagcaattc ttctctgcca
     8761 atccaaacgc gccttgagcg ttcctcaagc acgagtttcc cgctaccctt tagaaaggtg
     8821 gggtccaaat cctcaatggg cattgtggcg tctgaactga agaaggcgaa tttctggatc
     8881 aaggcgattt gaaaggtatc tccgtcaccc aggcgtgttg attcaatgag gggtgcacct
     8941 gaaagaaaag tgccattttt acttccgagg tcctcaatat aacagatacc gttttcgttt
     9001 cgcaaccggg cgtgatggcg ggaaacctgt cgctcttcaa tacggatggc gcaatcttca
     9061 tcacgaccta tatctatcgc ctcacggatg atccaccgcc tgccctcaag tggcccctgc
     9121 agtgctacta aaacgggaaa ttcatgagtg atagagttgc cgaatgaaga atttctcatg
     9181 cattcctcaa acatgattat acaagattgt cctcaggtat aatagcaata tgggtaacca
     9241 atccttctct ttcattaatt cggtaagttc ataaatgata tcacccctaa aaaaaggcaa
     9301 catcctgcgt gaaaggtata ctatccgccg cgttatcggt cagggtggca tgggttgtat
     9361 ctatctggcg gatgacctca ggctggaggg ccggcagtgc gcggttaagg aagtcgaaca
     9421 cgatcgaaca cttcccaaaa acctgttaaa agaagctcgc gaccaatttc aacgagaagc
     9481 cacagtattg gcgcgtcttg accatcccaa cctgccaaaa gtttccgatt acttctcaat
     9541 tgagaacagt gattatctcg tgatggattt tgtacccgga gaggatctac gcacgctgat
     9601 gctaaaacag cgtgaaaaaa atcagttcat acctgaaaaa gacgtgctca actgggcatt
     9661 tcagctggca gacgcgctcg aatacatgca caatcaaaac ccgccaatcc tgcaccgtga
     9721 tataaaaccc agcaacctta aagtgactcc agcagggctt ctcaaactgg ttgatttcgg
     9781 cctggtgaaa gtgctggccc ccggcgaagt caccattacc atccttcaag gtcaaggaac
     9841 ggcactttat accccgttgg agcagtacgg cggtgatatt ggtcataccg acgcgcgcag
     9901 tgatatctac gcttttgctg caacgttgta ccatttgctc actgccaaac ctccagtaga
     9961 tgcccgccag agattcatca gcccaaacgc tttacagcca atccgtgaaa ccaatcaggc
    10021 ggtcacccag aaaactgaaa atgctatttt atggggaatg gccttgcatc cggatgatcg
    10081 cccatccacc atcggtcaat ttcgcgaagc cctgattggc agccgccctg tcgcgattcc
    10141 gtcaggaaac aggttgtcag tgaaaattcc caccttctac aaccgtaaga ttgggaaaca
    10201 atggtacatt ctgatgggct tgagcctatt gaccctgctg gtgacactac tgcgctgagt
    10261 gctatttgcg cgttttccac cagtaaaaca aaaaaccact cccaaatccg cagaccaacc
    10321 cgatacctac gagaattaat atttgcccag atgcaacctc gttgagcggt gattttgagc
    10381 caggtaagcc caatgcagcc caaaggtacc ccgcataccc tcccgcacca gaaaacaaga
    10441 tggtgagaaa attccatttt gcgctgaggt gattgtgggt aaaataactc aagcctaggc
    10501 aaaaaccaac gatcaagata ttcgccagta accaatccca aaaagccact tttccctgtt
    10561 tgtgcaattg tataaccttg ctctctggtg taggtgaggg cgaagcttcc gtcggggtgg
    10621 ctatatctga acttactgga attggagttg gctcaaaaga agtaacgact cctccggtgt
    10681 ctgtaatatc aagcctcagg atctgagaga tgaatgcagg gtctgcagtc acgcttaatt
    10741 gcaggctgcc aatactggga atcacataca tcacacgagc aaagccatcg atcgtcctgg
    10801 cttcaagctg ttcaacagag ccactggttg accgggtatc aataatgaag cgcaccaggg
    10861 tcccatctgg cacaggattg ccattgtgat caacaatcac gccagccttg atgggtatcg
    10921 tatctcccgc cttatagagt aacggttggc tgtaacccgc caaaggtggt gcctgcggtt
    10981 catctatttc gacgggcgtc tcgatcacca aagggatcat ttgggttggg tctggtgaga
    11041 gcacgtaaac caggtcatat ccgattccgg gcacagacac gggcagcttg cctgagggcg
    11101 tcagctcctg catcaccaca cgcgaagcga tctcataaaa ctccggcgta ttgctataaa
    11161 gagcgtaata agccgttaat tttgaaatgt cagtggagtc aaggaagtag ggcgcgttaa
    11221 aggccagccc aatcagcttt ttatttctta ccagatcagg tctctcgtcg aacaagcgtt
    11281 tataggtggt tgcttctact ctttcagcgc caaattcagc gaatgaaaag atgatccatg
    11341 ttgcggcgtt taaagagacc agtatatctt ctgtcctatc tgcctgttcc agtaccatcc
    11401 tgaggttctc atagctgtag gcggtgaggt gactcgccgg aatgggatca cctgcgcccg
    11461 acccgtaaag ccggatgatt gaattcatca atcgggcagc aggaaaatca gtttcaaccg
    11521 ggcaactgct gcattgcgtg ttggtctgca catttgagat aaacacaatg cggtcatttg
    11581 ctcctggggg gtccggaaga acagtattaa gatcctccat ctcagggctg atcagcgtaa
    11641 cggcagattg cgcgatttcc atcacggttg cttgggcttt tcctacagtc tccagctggt
    11701 tgcggggtgt catcacatca gccggatcaa atgaagggta caatttcatc ttcaattgca
    11761 agatttttag aactgcctca tcaacacgat tgtgaaacgc gctgtcttct tcatactttt
    11821 gaacaaaata gtcaatggtt cttaccaccg ttgagtaatt atcttcatct cctgttgcca
    11881 ggatgtttcc catatagagc aggtcgtttc cagccatgag cgcgctgcga gcaacctgtc
    11941 ttgcatcaaa cccggtgttc aaagggtcta caaatttacg aatagactcg ctccccaaat
    12001 cgtcactgac gatcaacccg cccattttcc tccaggggct tagttctggc aggttcatga
    12061 tttgttccac agctactggg tcaaatgaga tgggtttggt tgaagagcga atattgccct
    12121 ggaacccctg gtaacgaatg tgagaaagca gcaacccatc caccaccccg atgtgacctt
    12181 gatcgatttt tgttacattg aaaaaaggcg ctaattctac acgcatcaat tggtcaagtg
    12241 attttctgac cgtggcgatt tcttcatccg gttgcctgtc tgagctgccg ctgcccggaa
    12301 agtgtgtggc gatcacagcc acacggttcg cgctgccagc ttgaaccccc tcaatgtagg
    12361 cttttcccag cttacccacc cagtaagggt ttccgccaaa tgtttttacg cccaggtcgt
    12421 ctctgccctc ggtatggatg atatccagca cgtccaatga aggcccaatc aacatattga
    12481 tccccaacgc ttcgagttct gagcccaata ccgacccaac tgcgctggcc tgttcagttt
    12541 tccaactggc accaattgtc atcaagttag gtagattggt aacgccatgc aggatttgtc
    12601 catctacatc caggtatcca ttttgcgtga tcgcaaccaa cagcggcgcg taagactcct
    12661 ggttttccac catctgagcg gccgcttgca ggtctgtaac cagttctttt aaaaccccaa
    12721 ttgtgttttc cggaccgata aaattatcat tatcctttct cagtaccact ccgccaatat
    12781 gcttttcttg aataagtctg actatccggc tgccctcctg cgctgaggac ccctggaaag
    12841 agatgagaaa aacctgccct actttttcct cagtggtcat ttgcgctagc cgaacctgcg
    12901 cagcggccag gcgcgcattc gtttgcgcgc ggtctcccgc ctgggcggtt gcggtaggcg
    12961 ctgccagact tagggcaaga aacagcagga aaattttttg caaggttttc ttcaagcgat
    13021 ttagccagcc ttactttgaa tgaataattt cattaaataa ttcggactga atattgccgc
    13081 cggtgaggat cagcgctgca ggaaacaact gaattttacc agcaagtcgc gcggccaatc
    13141 caactgctgc tgaaccttct attcttactc catatttctt ccagcaatac cggatggcta
    13201 aacggatttc ttcctcgctt accagtacga tatcatcaac aaatcgacgc atcaatggga
    13261 tggtgattga atcgtgggct atctcccctg ccagcccttc tgcgatactt tctgtttcgg
    13321 ttacaccagt ttgcgtgcct gaataataaa cctggtgagc ataaggagag gtttctgact
    13381 gcacgccaat taattggggt cgctccttca tattgcttaa atacacacca atgcctgaga
    13441 tcagtccacc ccctccaacc ggaacaacca gtgagcggat gccatccaat ccccccaatt
    13501 gttctaccag ttctggccct attgtcccct gccccgcaat cacctgcgcg tcgttataag
    13561 gagaaatgaa tgttttcccg ctagtttgag catgagccaa ggcctgtttt tcagcctcaa
    13621 catacccacc tctcaccagg cgaacatcag cgccaaactg gcgcatcgcg tcaatcttac
    13681 tcgcgctggt gttttcaggc acaaatacca tgcaggcgct cccccggctg gcagcagcaa
    13741 aggcaactcc ttgcccatgg ttccctgccg aacaggcgac caacccccct ttcaacgcac
    13801 tcttttccaa actcaatact ttattcgagg ctcctctcaa tttaaaagag cccgtctttt
    13861 gataattctc ccatttacat agaatgtcta attgtgggac tttggtaaca ggggtctgct
    13921 cgatgattgg ctggatgcgc ttcgctgcgt cgatcaccca ttcagccgtt atcactttcg
    13981 ctccaaaatc ctcaacgcct ttgcagggtc atcagtaaag aaaccatcca ccccatagtc
    14041 gatcatcctt cgcaaatcat tatcctcgtt gaccgtccaa acgtgcaccc ttcttcctcg
    14101 tcttctttct gtattcatca actgcggagt aacatcatcc aggtaggggt ggatgatttt
    14161 tggagacaac tggatgaata aggcggaacg agaagcaacg ccaagtagac cctgcgaagc
    14221 cagcagcgca accggaacat ctgggagcag tatcttcatc ttcttcaggt ttccaggaat
    14281 gaaggaggag aataatactt ctttctctaa ctggtgtcgc ttaacgatct cagccacgat
    14341 cggcaccagg ttatcatgac gggaagcgta attcgtcaat tccacgttga taaaaatgcg
    14401 tttaccgaca ctctcaaata cttcatccag tgtgggtatt tgttcccctg cgtaagcatc
    14461 aaagcggtat tgcgcgttca atcctttcag agaggctaag gtcatctggt ttacccgtcc
    14521 agaaccatct gtggtacggt caacagtttg atcgtggatg accaccgcct tcccatcagc
    14581 ggaaagcttt acgtccaact cgatcgcatc cgccccctgg cttaccgcca gttcaaaggc
    14641 tgcgagggta ttttccgggg cgtattgact ggcaccgcgg tgagcaaaaa caacaggctt
    14701 tggcaaatcg tcgagcattt acaactccac aaaataggat gaggctgctt gatcgatcat
    14761 ttcacgcgac atgaccggtt caatagagaa atagcgtgaa atatcaatga tctgatcgca
    14821 aagatctcga gggtgcgagg ctcgcagttt gcggttgtgt ttggcatacc attccttcaa
    14881 gaggtagttc aatccctcgt cagaaaacac aacccctttc tgcgcggcca cccgcatgaa
    14941 tatctcgcgg taatgcactg catcggggtc tgtgatctca attttgtgcc gcagtcgccg
    15001 caagaacgct tcatccacta aatcctttgg cggcaggttg gtggaaaaca tcaccagcac
    15061 atcaaaaggc acttcgatct ttcgccccgt gtgtaatgaa aggtagtcaa tgcgattttc
    15121 aagaggaaca atccagcggt tcagcaagtc gcgcgggcgc acctgctgcc ttccaaagtc
    15181 atcaattaag aggattccgc cgttcgcttt tacctgaaaa ggagcttcgt aaaacttcag
    15241 ggtttcgtta aaaaccaggt ccagtccggt cagcgtcaat tctccgccca ccataataaa
    15301 gggtctttga atcttcaccc agcgctgatc tttcctgcct ttcatccccc tgttttgcgg
    15361 gcctgtttcg tcttcggcag ccagctggtg gttcatagaa tcgaacatgg taatgacctg
    15421 gccgtcgaca aatatagcgt agggaatata cattgtttgc gtgagcacct ggttgccaat
    15481 cgcgcgggct acactggttt tcccgttccc tggtgcgccg taaatgaaaa tggaagtgcc
    15541 agaattaact gccgggccta atcgatggaa tacctcgtcg ccaaagacca ggtcgcccat
    15601 aatttgacgc atattacggc tggtcacaaa catacggccg cggctttgcc ggagcacgga
    15661 ttcattatag gcctcaagcg gcacaggcgc tggtccggcg tattggcagc gatcgagcgc
    15721 ttcgcgcgcg tgggtaatac ccgcgccggt gatcgcatac aaataagcgc cttcccctaa
    15781 ccccatctga gaagatttga cctctacaag cttatcccgt ttgagagcat ccagcagttg
    15841 gtccaccatc cctgtaaaag gcaggcataa agcttctgcc accttgtagc ctgtcatgaa
    15901 tccctggaaa tacaaaattt tcaaagccag gtcctgaagg aacagcgcgc tgagaccagt
    15961 atcttctatt gtattaatgg ggtttgggga gaaatggctg ctactactca cctcaggatt
    16021 aattctggga aatgatgagg actgagtttg atccgtcaca aagaccctcc gctgataatt
    16081 gaaaaggatg aatgcagtaa aaatgattat agcatgccag caagaggatt aattcacttt
    16141 cggttgactg gtactcttcc tttcaaatct gatatttaca atgaccgccc ctaagaaggc
    16201 tagagccagg cctataatgc ccgccaagtc ctgggtttcc ttcaggaaga tccaggcgaa
    16261 gatggcgacg aaaaccagca tggtgccgtt gatgatggca gattccatgg cattcaacgt
    16321 ctgtaatgtg taattccaca caacaaaagt gaaggcggtg ttaaccagcg ccagcaccag
    16381 aatgatcacc caactttgaa gcccgagatg aggtaaaccc tgccataaga tgcctgccac
    16441 caacatcggg atcgagccga ttcccatggc tgtaagggtt actgcgatgg ggtttaccct
    16501 cccgcctgaa ttgatcttgc gcgtcacaac cgttccaatg gcgttcgcca gcaggcagat
    16561 caccccaaaa acaaacccga gggtggaatt gcctgagaag ccatcagggt aaaagaaaac
    16621 gaacatgcca gccaggttga tcagcacacc cagcacctga accttcgttg gaagctcctt
    16681 aatgagaaaa atcgctgaaa tcgtgaccaa tatcgaggtc aggttgagta acaggctgag
    16741 tgtggtcgcc ggcaggtaat caaggctcaa gaattgagtt ccctgagcaa caaaatacga
    16801 aaagacaccc atcaggatga tcagccgcca gtcactcttg ttgaaatcac ggatctgttg
    16861 tctcaccttt ttctggaaga tgaaaggcag taaaaataga aaagccaaaa aataccttag
    16921 cccggcaaaa gtgagcgcag ggatgtcttt aaggccaatt ttgatcagca cccaggaaga
    16981 agcccagatg atcaccacca gaaaattcaa cagaatggct ttgattctgc tcggtgtttt
    17041 ttccatatca gaaactgacc gctgaaatct tcatatctcg gatgttaagc tgcaaagtgt
    17101 ccataccgtt gtagtggtta acctcaacag tataggcaag atcaaagcgc ggtttttctt
    17161 cataccaggt ctggtaccag gcggcctggt taaacgccac cgcccgattg atctggcttc
    17221 ctcgaaccga aaagctgagg tgcgtgcttt cctttcccat cacctgcatg cggtccacca
    17281 ctaccccgcg ggagataaac aatggcgcgg ggtttcccat tccggttggt tgcaactggt
    17341 cgatcatcct gtagtattca ggtttgaacc ggcttaactc gatttccata tcagccctga
    17401 gtgttgggcg taaatcttgc gcgctcaatt cgcgggccgc gatctcttgt aagcgcgcga
    17461 tcagctggtc ctcgttctcc atcttcacgg tgaaacccgc tgctagctca tgtccgccgt
    17521 gccgcacgag caagtcagcg cattcatcca atgctttggt gatatgaaag ccattaatac
    17581 ttcgacagga agcacggatg gtatccccta accgttctcc cacaatggcg gggcggtgaa
    17641 atttttcaac cagcttcgat gccaccaagc caacgatccc tgagctgaac tcggaatcaa
    17701 acgcggtgat aaggtaagtc gattccaggt gggtcacagc gccctgcgct ttttcttgtg
    17761 ctgccagcgt ggctttttgt ctttctgaat tctgattatc caatacctga gccgctttga
    17821 ccgcgtctac ggctgatttc gaaaccaaca actcataagc ctgcagggca gattccatcc
    17881 tgccagcagc gttcaatctt ggtcccagga tgaagccaat atccgtggcg gtgacctgct
    17941 gtacatttcg tcccgatacc ccgatcaagg cgtttaaacc cactcggctg ccattcctga
    18001 ggtgattaag tcccctcgcg accataatac gattttcacc ggttaacggg agcatatcag
    18061 caaccgttcc cagcgccacc aggtctaacc actcatctgc cttgccaacg tccatccctt
    18121 ttttgataaa taaagcctgg gcgattttgt aggccactcc caccccagcc aggtctttgt
    18181 acgggtacgg atcacgttcc tgtttggcac aaagaaccgc gtaccccgcg ggcagatctc
    18241 cttttggaaa gtggtggtcg ctgatgatca aatcgatacc caaattgctg gcggtaatgg
    18301 cttctcgcgg tgagcggata ccacaatcaa cggttaaaat aactttcgcg ctatgctcac
    18361 tggccagcgt ttgaatggcc tcgtcattga gcccgtaacc ttcttcaaac cgattaggga
    18421 tgtagggttc cacgtttccg cccaaccgct gcagcacctc taccatcatc acggtcgcag
    18481 tgacaccatc cacatcgtaa tccccataga caacaatttt ttcaccgttg tctattgccg
    18541 cgaccagtcg atcaactgct gactccattc cgagcaataa aaaaggatct gccaattcgc
    18601 catcattgga aagatactgc tgggcgccct gagcggtacg aatgccgcgg ttgtaaagca
    18661 gttgccgaaa aaatggcggg taatctgcga gcgcttcatc cgccattcca tcgatttttg
    18721 gagcaatttc ccaccttttc gttagatttc tttcagccat cgaaaagtcc ttttttatct
    18781 agcgatctct tccatcaata aatgatcaat taggccaggt aaattccgct caagtataaa
    18841 cgagcagcca atcacccgca aggcggttat cgtcttcagc cccatcaagt aaaatagaag
    18901 gatggatagg atgaaaaaat gacccaggac caaaccttca cgcttattat cctgctcgcc
    18961 gcggtcattc tgttcgcgtc gaaccgtgtt cgcgcggaca tgatcgctct catcgtgatg
    19021 gtatcccttg gtctaacagg agtgaccagc gctgatgagg ctttttctgg cttcagcagt
    19081 tccgctgtga tgaccatcct tggcatctcg atcatctcca tcgccctaca aatcaccggg
    19141 gcaaccaatt cacttggcaa tattctccac acggctggta aaggcaatga gaaattggtc
    19201 attctcctcg ttaccttgtt tggcgccctg gtatctcttt ttatgaacaa catcgccgct
    19261 gtcggagtgc tgctcccggc agtgatgacc ctttctcgcc gcagtaagat ctcgccctca
    19321 cgcctgctgc tgccgctggc atatggcacc attttggggg gtatggcgac gctcttaact
    19381 acttcgaata ttatcgtcag cggcgccctc aaagacgcgg ggctgccgta tttcgggctg
    19441 ctggattatt ttccgattgg cgggcctgta atgctgatgg gaattcttta cctgtccttc
    19501 atcggcttcc gtttgctccc ggcgaaatct ccaaccaata aaattgagga tagacgtgag
    19561 ttcacagaga aattaagcga tttttaccag gtggggaaga acctggtgaa aattgaactc
    19621 ctgccccagt ctgaaatggc cggcaagacc atcgccaacg ggcactggta taaccgggtg
    19681 catgtgaata ttctcgcagt actgagagaa aaagaaaata tcttttcccc cgacccctgc
    19741 acgatgctta aaccaggcga tattttaatt gcccgcggaa aagtcacaga gcaatccgca
    19801 aaggacctga acctgcgggt ttccgcaatc aataaccagg aaatcaagat cacagatgaa
    19861 gcccacccac tggcagaggt cattatttct cctcattcgc gctttattgg caaatcgctc
    19921 cagcagatgg actttcgcga gcgttatcag ttaaatgtgg tgggagtctg gcgcggcggc
    19981 aaaccggtag aaggagagta tgctgacctg agcctccact ttggtgatgc gcttctcgtt
    20041 caaggccctt ccattcaaat ccgcaacctc aagaaaagcg aagaccttgt tgttctcgag
    20101 gaagatccgg acgcggtgct tcttccccgt aaaggcctgt tgaccatctt catcactctc
    20161 ctgaccctca ccatcgctgg gctgggtttt ctgccggtgg cgctggttgt aatgggaggg
    20221 gctgtgctgc tcatcctcac tggctgcgtc gggttgaacg atgcgttccg cagtattgaa
    20281 tggaaagcca ttctgcttat cgccggtttg tggccaatga gcatcgcgct tcagtccacc
    20341 gggttggctg aaatggccgt aaacagcctt ctacgcctaa gcggcggctt acctcccttg
    20401 cagctggccg cactgttcct gttcgtggct gcattgctca cacaactgat gagcggacag
    20461 gtcaccgcca tcgtcatgat cccgctggcg ctcgtttcag ctcagcgcat ggggatcgac
    20521 ccgcgacctt tcgccatggc ggttgctatg ggctgttcgc tggcttttat ttctcctctg
    20581 ggacatcccg tcaacattat cgtgatgaat ccaggcgggt acacgcttaa agactatgtg
    20641 aaggtgggtc ttccgctcac aatcgcggct tttttgtgcg ttattttcgc gatcaagctc
    20701 tattgggggc tataatacaa ctccatggaa caaatcgact tctcttatac cctgctcaag
    20761 gagcagggat cggccagggc tgggattttc ttcaccccgc acgggccgat tgagacacct
    20821 gttttcgccc ccgtaggcac gcaggctaca gtcaaagccg ttacccccgc gcagctggag
    20881 caaaccggcg ccacactggt actggccaat acctaccacc tttatctgcg ccccggcgcg
    20941 gagttggtgg cagcccacgg cggtttgcac tcgttcatgc aatggcaaaa acccctgctc
    21001 acagactccg gcgggttcca ggtattttca ctctccggct cgcggaaggt ggatgaggac
    21061 ggcgtgacct ttaaaagcca catcgacggt tccatgcacc ggtttacgcc tgaaagctcc
    21121 atacagattc aagaactgct cggcgcggat atcatcatgg ctttcgacga gtgcgccgtg
    21181 cctgacgacc tgctttacag ccgcgtcgcg ctcgaacgaa cccacgcctg gctggagcgc
    21241 tgcatccgtc agaaaaaacg ccccgaccag gcgctgttcg gcatcgtcca gggtggtgtt
    21301 ttcgcggaac tgcgccgcga atcagcccgc ttcgtctctt cgtttgacct acccggcgtc
    21361 gccatcggcg ggctttccgt cggcgaaacc aaggcgcaga tgcacgcggc gctcgaagag
    21421 gtcacacctc tcttgccgcg aaccaaaccc cgctacctga tgggcgtcgg ctctcccgaa
    21481 gacctggtga acggcatcct gcgcggcgtg gatatcttcg actgcgtctt gcccacccgg
    21541 ctggcccgcc accaggcggc gttcacgccg aacggacgcg tcaacctgat gaacaggatg
    21601 ttcgcgcatg atttcaaccc catcgatgag agctgccatt gttacacctg ccagcacttt
    21661 tcacgcgcct acctgcgtca tctgatcgta gccaaagaaa tgctctcagc caccctcatt
    21721 tcaattcaca acattcagtt cctcgtagac ctggtgaagc gtgcccgcaa ggcgatcctg
    21781 gagggcagtt tcccgctttt cgcctcctcc ttcctggaag cctatcaaat caaggagcct
    21841 tcatgagcct tatccaatct ctcatcctgg gaatcatcca gggtttcact gagtttttac
    21901 ccatctcttc atccgcgcac ctggtgcttg tgcccttcct cttcaactgg gccatccctg
    21961 agaaccaggt tttccccttt gacgtgttgg ttcagctggg taccctggca gcggtgattc
    22021 tctatttctg gaaagacctg tggaagatca tcaaagcctt tgtgaccggg ctcatccggc
    22081 gccagccttt cgcggaggaa gacgcgcgca tgggctggta cctgttgctc gccacgctcc
    22141 cggctggctt cgcgggtatt ttcctcaaag agagggttga agccgccttc aacaatccca
    22201 ggctgaccgc tattttcctg ttcgtcactg ccgtcttcct ggtcggagct gagttcttcg
    22261 ggcgccgcag ccgccagctg gacaaaatga cctggaaaga cgcgttatgg gtgggcgtct
    22321 ttcaggcgtt ctccattttc cccggcgttt cacgctccgg ttcaaccatt gccggcggaa
    22381 tgacgcgcaa tttcaaacgc cctgcggcgg cgcgtttcgc cttcctcatg tccattccaa
    22441 tcatgctcgc tgcgggcatc ttcagtctgc cagatctctt tgccgttcct gatttgcccg
    22501 cctttctgcc catcgtgctg gttgggttaa tcgccgccgg tgtggttggg tacctttcaa
    22561 tccgctggct gctgtccttc ctggtcaaac gctcgctgat tattttcgct gtgtattgca
    22621 ttttgttggg ttccgccgta ctcattatca gcggtattcg tcaaaccagc cccaatgtgg
    22681 ctggcactgc gccaggcgcg cctgccgcgg aaagcgcgga gtatcctccg cagacgccaa
    22741 tttccatcca agaaacactg ccgctggaaa tctcacccgc gttggactgg ctgcgaccgc
    22801 gcatcagcgg ctgcgctgac gccatccccg gcctggcgct cgtgaccgaa ggcacggttc
    22861 cagccaacgg cgaacccatg cagctgagct ggggtgagcc gccaaacctg aaaaggaaag
    22921 cagtgatcat cggagaagac gacctggttt tcatcgtcaa cccgcgcaac ccgctgagag
    22981 agctgcccct cgcgctgctg cagcccctgc tttctggtga actcaattcc tggggagaag
    23041 ttcaggttgc ctgccctgag tgtttttccg ccggtcaact ggatgagctc atcgaccagc
    23101 ctgtggaagt gctgctctac cctgccaatg tggatgcgcg ccgcgtgatg gacgaagccc
    23161 tttcatccgc gcctgtgcgc ccaaatgcct cttccgtcct cgtgccttca gcggagatga
    23221 tgaagcaaga agtctcctcc gccctcaacg cctttggcac cctaccctcg cgggcggttg
    23281 acgaaacggt caaggtcatc cccatccaaa acggcgccga aacgctcaaa attacccgcc
    23341 cgctgctcgc caccagcgcc gccgaagcac agcccctgac ccttcagttt ctcacctgcc
    23401 tgcaggcggc tattacgccc tgagctcgcg ccaccttcat cgctcgttcc tctgcatcct
    23461 caggagataa cctggtcgcc tggtaaatgg atctgtcgcg cttacgcggt ttccaggtaa
    23521 cgcgccagtt cccannnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    23581 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnctggta
    23641 aatggatctg tcgcgcttac gcggtttcca ggtaacgcgc cagttcccaa tcggtcacat
    23701 ccaggcggta gcgctcccat tctgcccatt tcgcgcgtct gaaagcgttg tagagggtct
    23761 cacccaaagc ctgctggatg acggaatcct tttcaagttc caccaacgcc tcgcgcagcg
    23821 accccggcag ttccacaatg ttcaggttct ggcgttcttc gcgtgatagt tcgtacacat
    23881 tcacatcgtt cagcggttca ggaggggtaa gttgctgctt catcccgtcc agggctgccg
    23941 ccagcatgct ggaatacgcc aggtaagggt tgcaggatgg atcggggcag cgtagctccg
    24001 cacgcaccga tgtggtcatc ccctccagcg gacgggggat gcggatgagc gcggaacggt
    24061 tggtttgcgc ccagcccacg tacaccggcg cttcataccc tggcaccagg cgcttgtacg
    24121 agttgaccgt gggcgcagtc accgccgaaa aagctcgcgc gtgcgccagc tgcccggcga
    24181 tgaacccata agccagcggc gagaggtgat actcgtccgc tgcgtcgtag aacaggttgt
    24241 tgccccgcag gtcaaagagc gactggtggc agtgcatccc cgaaccgtta atgccaaaaa
    24301 tcggcttggg catgaaggaa gcgatcaagc cgttctgcgc ggcgatggct ttgaccgtgt
    24361 atttgagcgt gagcacgttg tccgcggagc gcagggcgtt gctgaagtga aaatcaattt
    24421 cgtgctgccc cagggcgcac tcgtgatgtc ccacttccac ctccaacccc atggagtcca
    24481 acgcctgcat caattcggta cgcacgcgca ccgcctcatc gttgggcgaa aagtcgaagt
    24541 aaccgcccac atcatgcggc acagggtgga tatcgctgct gccgttgcgg cggaagagga
    24601 agaactcggg ttcagaaccg accattaaac gcatgccgcg ctcttcaagc gccgccagct
    24661 gccggctcag gttaccgcga gggtcgccag cgaacggcgt gccatccggg ttgtagatgt
    24721 tgcagaaaag gcgcgcgcgg cgcatgttct ctggcgacca gggcagcacc gcgtaggtgt
    24781 caatatctgg tttcaggcgc atatcacttt cctggatgcg cgcgaatccc tcgaccgaag
    24841 agccgtcaaa ccagatgccc tgattgaccg cgccctccag ccgtgagatc ggcgcgtcta
    24901 cgctttttac cgtaccactg acatcggaaa actgaaagga gatgaaccgc acccggtctt
    24961 ccgccacccg ctctaaaagt tctttcttgt ccattgttgc gctcctattt aatgaattca
    25021 ggcatgcgtg ggggcatgcc ttttttcaga cgcgcttaca gcagcccttc cgcttaaaac
    25081 cacaggcacg ccatcctgcc tggccggtat cagctatctg tgccgcgcgt cgccgcgtgg
    25141 ttttgttcgc tgacttgcat gggaagggag atggaagccc ctggcagggc tccggaggca
    25201 tccgctgatc gttcgatttt cccctatgcg atccccccag ggttaacatc cttctcgcga
    25261 aggagaacct cctcctcgtc atctcggcga accgagatgc agccgctgtt tctctcttta
    25321 ttcaattgtg aatggcgcct atcatacaga ggtcgcgagg aaatgtcaat acccgggttt
    25381 gctgcttaag caaagagcaa ggtcaacctc cagcggcggg tatggcgtgg tgcgcggagc
    25441 ctcctcatcc gcgatattca tcgcgcaccc cgccgcttgc ctttcttgaa caaatggtct
    25501 ataatcagcg tggcgcatcc atactataat caaaagaggg catttccccg cagcttgctg
    25561 cgaaaaatag taagattaaa ggatgagtga gacttggagg aagagtcaca acatatcgat
    25621 tttgatttac cactttgtat gtccagcaaa gtatcggaaa gtagtgttca acgacgaagt
    25681 aggattacgc aacctttttt ctgtaaattc gagtcagaaa tacctatcgt tcttta
//
LOCUS       DAOTLW010000030        24853 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_2336_length_24853_cov_11.359102, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000030 DAOTLW010000000
VERSION     DAOTLW010000030.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24853)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24853)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24853
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2336_length_24853_cov_11.359102"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="P5516_06005"
     CDS             complement(<1..248)
                     /locus_tag="P5516_06005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92027.1"
                     /translation="MTLQRHTPVEASQAGKDHLWLQISSLPYFRALLRAVEARFYENI
                     DLPAPALDLGCGDGHFASLAFNRALEVGLDPWEKPLREA"
     gene            complement(406..1236)
                     /locus_tag="P5516_06010"
     CDS             complement(406..1236)
                     /locus_tag="P5516_06010"
                     /inference="COORDINATES: protein motif:HMM:NF014754.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HRT92028.1"
                     /translation="MTNQSIDLAQLFGSVVSTLDQHKAALNQADTYNHNHGDNMVEIF
                     EVITQAMKAKSNATPAEQLAYASQLLQAKSQSSSAKLYAQGLQDASAQLGGKKTLTTN
                     NALTLLQALMGTQKKVTPQTLQTQARKTFEQQPSGQSAGGDLLGSLLGGLAGAQQTPS
                     GAGAGGDLLGSLLGGLAGAQQAPAGAGTGDDLLSSLLGGLTGAAAPSGSKPVKIDTAQ
                     LLKAGMAYMQAKQSGSSDINALLSALMAGSRMGTQNYRTQSGTLVANSLLKALAQMSQ
                     "
     gene            1348..1917
                     /locus_tag="P5516_06015"
     CDS             1348..1917
                     /locus_tag="P5516_06015"
                     /inference="COORDINATES: protein motif:HMM:NF015513.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HRT92029.1"
                     /translation="MNDKKLPLNASEYALLGFLKAGPCHGYELHRKLTEPDGAGMIWG
                     IKISNMYAQLDKLERRGLISGRLLENEQRPARTQYSLTAAGKQLFEEWLHQPVQHPRD
                     FRHFFFLQLFFLSQYEPDALESLVERQLELCQAWQQNLLAAQERSSTSVDFADLTNRF
                     RHSQIQSMIDWLFWIKSEKIKIKPKRGNQ"
     gene            1914..3077
                     /locus_tag="P5516_06020"
     CDS             1914..3077
                     /locus_tag="P5516_06020"
                     /inference="COORDINATES: protein motif:HMM:NF013648.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT92030.1"
                     /translation="MKRPLQFLVVLVLFSSLLAACAAPTPAAAPSLTEPPATEAPAAV
                     STPAPTEPPEMEKITVVDALGREVVFDQPPSRIMIVGKAAFMLLDAAFLFPEAPEKII
                     GYELRSQTGLDFIHTAFPQSHNMIVLEMDAGPEQIAPANPDLVLMKSYLKEQLGDQVE
                     LLGIKVVYLDLETPETINQDIRTLGKLFGNTERAEQLVTLYDQARAKIADITNVIPEE
                     EKPSTLLLQYSDKGGEVAFSVPTGGFLQTQLVEMAGGAPVWKEVTGKGWSVITLEQIA
                     AWNPEIIIVVDYRGKAMEAVDRLKADENWQQLDAVKNEKLYAFPLDFQSWDQPDSRWT
                     LGLTWLATIMQPERFASVKMHEEIPAFYTDFYGLSESVIEQSILPLVKGSFLE"
     gene            3116..4108
                     /locus_tag="P5516_06025"
     CDS             3116..4108
                     /locus_tag="P5516_06025"
                     /inference="COORDINATES: protein motif:HMM:NF013219.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRT92031.1"
                     /translation="MKNNKNLTAFVLVVTPLIVLGISLFIGRYPKPLLMPLSVLLHDQ
                     LGQNLVYNLRLPRLITSALLGMSLATAGGVMQMLFRNPLVEPGFLGVTQGAGFGAALS
                     ILFLTTAPLVVELSAAGFAIIGLLLSYFIARRIRFGGWVLRLVLSGIAVSAIFSAGLG
                     ILKYMADPTSQLPEIVFWLLGGLWSVTWTDLWYILPVVVIGLVIIYLMRWRLNLLSLS
                     DETAFSLGVAVGRERTLLLAASVAITAVVVSVAGIVGWIGLIIPHIARRIAGANAASF
                     IPVSMSLGAVFGIICDDLARVLTTGEVPLGIITSLIGAVIFLIMMTSRSMRIAR"
     gene            4105..4929
                     /locus_tag="P5516_06030"
     CDS             4105..4929
                     /locus_tag="P5516_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92032.1"
                     /translation="MNPAVIQVNDLSFSYNPAGNAAVHQVSFEILPQTVTAILGPNGA
                     GKTTLLHLLLRLKTPLTGSIHLQGRPLNHYSRHELSQNIGLVPQTEYIPFEYTALEYV
                     TLGRAPYLGPLEIPAEKDTEIARQSLKEVGVSHLENRPIPALSGGELQLVLLARSLCQ
                     QPRVLLLDEPTSHLDLANRNITLQIMDRLRRAGTTLVFSTHDPEAAALIADKLVLMRA
                     GQVLETGSLEEVFTTETLSRTYGTRVEVLQVDGLRVVKSIERLGGEQNGQNNPAER"
     gene            4901..5428
                     /locus_tag="P5516_06035"
     CDS             4901..5428
                     /locus_tag="P5516_06035"
                     /inference="COORDINATES: protein motif:HMM:NF015239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017111 - ribonucleoside triphosphate
                     phosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-triphosphatase"
                     /protein_id="HRT92033.1"
                     /translation="MAKIILLSAEKGSGKTTALLKLREKLTGLTLNSRGVLSPPVMVN
                     GEKIAIELMDARSAERKRLAVPAALDSPEASGLRWHFFEDTLDWGNHLLSEAVPCDIL
                     FVDEMGPLEFDRNEGLTKGFFAIDSRRFLVALVTIRPALLERALARWSDAQVCKVTIK
                     DQPHLVNNLFRLITG"
     gene            5491..6312
                     /locus_tag="P5516_06040"
     CDS             5491..6312
                     /locus_tag="P5516_06040"
                     /inference="COORDINATES: protein motif:HMM:NF013862.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HRT92034.1"
                     /translation="MNPISELLSKVKTLFPKKVIGLALSGGATFGAAHVGVLQVLEEN
                     GIRPHLVAGTSAGALIGAAYCAGIPLSEIETLFRTMNWPTLLRLSIRNSLSIFDTQPM
                     EEFLRKKIGDIEFQELRIPFAAVACDIHTGARVILDRGALAPAIRASAAIPGLFSPVE
                     VNGRLLVDGGIVDNLPVEQVRDMGARYVISSDVSHRGPNSKKPENLFEIMLSMIYIMQ
                     SRAALPLEDASDCYIRPQVSQYSSWGFKDVPQVMEAGRKAARLVIPQLRRQLRIR"
     gene            complement(6419..7570)
                     /locus_tag="P5516_06045"
     CDS             complement(6419..7570)
                     /locus_tag="P5516_06045"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRT92035.1"
                     /translation="MAQTIDAFARYDRRRERGVPTYLQEKWNGLSELNRFLAGSENLE
                     ELAVSASRRIVEILEVDFCQILLMDLDGQCNCRARYSAREMLIKKKASLDPIEIENIC
                     HRFLDAENNTRLFLGMEHLSERERKSLGISNQDISWIIPMRVETNPIGVLILGQSASQ
                     QEQILTPDSYYLVDLIADQLASAVFRAQMNERIESVSIETVIVLSKALETRDLHSASH
                     SKRMAALSEQLAKEFGFSARETRELCWAALLHDIGKIGVKDTILKKKGPLTEEEWAII
                     KTHPEIGAQIVRGVTGLEKIAPLILAHHERMDGKGYPNGLRGDEIPLGARILAVTDAY
                     TTMTDGRPYQKKRKHQEALQELLEQAGSAYDREVVQLFIQLFNKKDSLE"
     gene            complement(7647..7931)
                     /locus_tag="P5516_06050"
     CDS             complement(7647..7931)
                     /locus_tag="P5516_06050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92036.1"
                     /translation="MSNIFHDYHHHDETVVQKEREYSRILTAAVVNEKFRRLLLTNPN
                     MAIKKGFAGEAFHLGAEETRRISAIRVSTLAEFARQMNAEMTRPSLNLAE"
     gene            complement(7941..8516)
                     /locus_tag="P5516_06055"
     CDS             complement(7941..8516)
                     /locus_tag="P5516_06055"
                     /inference="COORDINATES: protein motif:HMM:NF036410.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5317 domain-containing protein"
                     /protein_id="HRT92037.1"
                     /translation="MILVVAVLVGLAAGLCRAKYGKREYRGYDLKAAGLVLLAFIPQG
                     FIFFLPSTRTRIPDETASFLFIISLVVLIIFSLFNIRKPSFWPITGGFLLNALVILLN
                     GGWMPISPEMVQRLLPDSTAGDWMVGERLGYSKDIVLLPENTRLWFLSDRFSLPDWIN
                     YQVAFSPGDIILFVGIVWLLWSLGGPKSVNV"
     assembly_gap    8647..8746
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <8747..9002
                     /locus_tag="P5516_06060"
     CDS             <8747..9002
                     /locus_tag="P5516_06060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92038.1"
                     /translation="HEEDEPCFVGNGFHERLLFYSYHFVRTALKAHRLAAARTIEMDL
                     SANSAGGERSARCQGEQYQHEHEEDEPCFVGNGFHERLLK"
     gene            complement(9178..12414)
                     /locus_tag="P5516_06065"
     CDS             complement(9178..12414)
                     /locus_tag="P5516_06065"
                     /inference="COORDINATES: protein motif:HMM:NF037660.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BTAD domain-containing putative transcriptional
                     regulator"
                     /protein_id="HRT92039.1"
                     /translation="MPPDLPVIRTKTLIPRRRSEILSRPRLLAALDNLLDLKLLIVAA
                     PAGYGKTSLLVDFSHHTQIPVCWYALDSLDSDPKRFIAHFIAAIQGKFPAFGQMASAA
                     LAELSQDNLNLDLVISAIVNDAYENISEHFIFVLDDYHLVRDVKPIEVFINRIIQESA
                     ENCHYVIASRTLLTLPDLSLLVARSEVDGLSFEELAFLPEEIKQLMAVNYHQSISDQK
                     AEEMITRTEGWITGLLLTAQVSPKGPYERSRIEKVSGVGIYEYLTQQVFDRQPLAMQS
                     FLLRTSLLEEFDANLCEKIVGKALGLEGQNWDEVIEKIQRDNLFVLPVGDETLFLRYH
                     HLFRDFLQNRMRVEKPEETLRIETELVAYYESVQEWERAIAILLRNGTPEQRVEMVRK
                     AAPAMILSGRLVTLTDWLNALPFDLVENKPELVSIRGTIAMLRGDYEVSLELLSRAIK
                     ELRAVGFAEELAAALIRRSAVNRHTGHYENALKDAQDAIELTRPPFGAPQKYAEALRA
                     EGLVYLQTGELNDAQWKLRESYRRFQDLGLESDAAKVLMDLGVIYSARADFEQSEKCY
                     STSLEYWQAVQNPLWLANVLNNIGMIQYLRNQFEQAVITLERSLSYSRLASPRIEGYV
                     LTSLGDLYRDVRALEEARQAYRQAEEVLQKTSDLSLDVYLTLALATLDRITGYLSSAQ
                     EQISAALEKAQKSGSKYEHHLCLLEQAILDFKNQKPADLAHTFEQLEDYFTAAGYQME
                     RFKAAVYRNLASLVETARTHTKLVIDPEMQKSEGSMRQVLIQILMEFGSYLELINPED
                     EQYPVVTQLKSEVAAFEKHLAQIHRVVRRHSSAVDFSNPKLSISGLGRMQVRLGNRVV
                     TNKDWKTHTVRDLFFFLLSHPEGATKEEVGAAFWPDADRDTVKLRFKNSIYRLRRAAG
                     SDCIAFDDDIYRFNRSMDYEYDLDTFMNELENAKKAQSTEEKIEHLRAAVSCYHGPFL
                     PKLDQEWVLIAREKFHTTFLNAAQALINLYMKVGKYSQAATLANRALEEDNYNESAYR
                     SAMLAYSAMNDRSAVARQFERCKLVLKKELDIEPSPQTVKLFHSLMQQ"
     gene            complement(12572..12949)
                     /locus_tag="P5516_06070"
     CDS             complement(12572..12949)
                     /locus_tag="P5516_06070"
                     /inference="COORDINATES: protein motif:HMM:NF039825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5684 domain-containing protein"
                     /protein_id="HRT92040.1"
                     /translation="MDYNYDGGLGFGYYLFLLLVSVFSIVCLWKVFVKAGKPGWAAII
                     PIYNIITELEIIGRPWWWLLLLFIPLVNIVIGIIVIFDLAKVFGKSTGFGFGLLFLGV
                     IFMAILAFGDAKYLGPIAGQPAV"
     gene            13042..13836
                     /locus_tag="P5516_06075"
     CDS             13042..13836
                     /locus_tag="P5516_06075"
                     /inference="COORDINATES: protein motif:HMM:NF014724.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HRT92041.1"
                     /translation="MSIFLSKFLPLFLYPAGLVTLLILLTLVFWKKRRAAFTFLVLAF
                     FILLLAGNKYVATAFARTLEWRYPPLVENTTADVIVVLGGGTEPAIEPRRMVELNAAA
                     DRVLYGIKLYQEKAAPMLLLSGGDIDFIGDSPSTPADDMATLMEMLGIPREKMIIQNA
                     SLNTQQDAEFSCELIKKNGFKKVILVTSAFHMPRSVALFEKQGCPVVPAPVDFSITQL
                     SWDNLTHPTTAEFILDLLPSYSHISTVTKTLKEYFGIWYYKLSGVL"
     gene            13866..14591
                     /locus_tag="P5516_06080"
     CDS             13866..14591
                     /locus_tag="P5516_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92042.1"
                     /translation="MKPKFIALALISLLLVACSLPKSLTSIIQRFRSQPAAVAESNSL
                     LNLVENGKVSVVGIHGNVGLGTFTGRTIDLQLQNNTDSKLDVLIPCGLVFIADDEKSS
                     RMMVVQPTVTSLNKGKTAVVKPFVLSIDALKGLPSPEKTYSVAELEEGKNKDFATCLC
                     EQDLPAETETKDLLDLQLAAWMVSGEGLLSNIPVDLNDLFKDFSGLPITIPGLDEVMK
                     DVAGNVAPNAQAWLDKCGISFGK"
     gene            complement(14651..15796)
                     /gene="phnE"
                     /locus_tag="P5516_06085"
     CDS             complement(14651..15796)
                     /gene="phnE"
                     /locus_tag="P5516_06085"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRT92043.1"
                     /translation="METLKSLLHSLKRVLIIIAVIALLVLSIRVTKPDLVKLVSSFPK
                     AKSIMGQLLRPEILGRESEKITVAQLVPVPCGSVANPEVPASGPRITVDPPCGNPKDK
                     VQVRGYELPKNAELSIRWILPDGGLLSIKNAVTNANGELTETMELRPITAAVDGKPAQ
                     LAFEITLPEGKVVASQTLKDVLDAMFVTIFMALIATTIGTIFAVPLSFLAASNITRKG
                     WLGSAVYYIVRAFLNIIRSYEPLVLATIFAMIVGFGSPFAGVIALSITTAASLGKMFS
                     ESVEGIDSGPIEAVTATGANKLQVVVYAVVPQIIPDFLSFTIYHWDINVRISTIIGFV
                     GGGGIGYFLSQRINTLQYSQSGTALLAIILVVWALDFLSSEIRKQLV"
     gene            complement(15798..17159)
                     /gene="phnE"
                     /locus_tag="P5516_06090"
     CDS             complement(15798..17159)
                     /gene="phnE"
                     /locus_tag="P5516_06090"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter, permease protein
                     PhnE"
                     /protein_id="HRT92044.1"
                     /translation="MKEKTSRSRLVSIFLSALVPGLGQLIQKKYRAAIAIFLAVATSI
                     AVVAWYGKPAWYILPALIWLWNIWDASSLPGGKNAVLPILLWLLMAYGIGGQVTEFNL
                     RALVENSKRSKDIVSAMFQLDFTQPKTEKKEGFTDIQSPCSATPPVPDRTVNGVRVYV
                     VEPCATVNQTITVKGEGFWPNTPTLIYWQNPIGGKAMDAQVKSDAQGNVSLEWVVSPY
                     VHSTAPDPTVPQIHRIKVSQSRPIGGIELSDVGGYVVQGIYETLALAFLSTIVGALIA
                     IPFGFLAARNLMTGNPVTTAIFLVTRTILNIVRSIESLIIAIIFVIIVGLGPFPGMIA
                     ITIHTVAALGKLYSEVIEGIDSGPIDAVKATGASWPQVVRYAVIPQVVPTFTALTIYR
                     WDINIRSSTIIGFVGGGGIGFFLWQWIVLQDYRAVGTAFVAIAVVVIILDFFSARIRE
                     RLV"
     gene            complement(17165..17932)
                     /gene="phnC"
                     /locus_tag="P5516_06095"
     CDS             complement(17165..17932)
                     /gene="phnC"
                     /locus_tag="P5516_06095"
                     /EC_number="7.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015416 - ABC-type phosphonate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015716 - organic phosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonate ABC transporter ATP-binding protein"
                     /protein_id="HRT92045.1"
                     /translation="MLKVEHLTKVYPNGTVALKDLSFEVPDGEFLVIIGLSGSGKSTL
                     LRCINRLIDPTEGKITMDGIDITAAKGAELRHIRRQIGMIFQHFNLVKRSSVMTNVLS
                     GRLGYLNSWSSTLGYFPHEEKERALAAMTRVGIADKVHSRADALSGGQQQRVGIARTL
                     MQEPKLILADEPVASLDPVLAHSIMQYIEKLNEEDNVSVICSLHYLDLIQRYAERVIG
                     LKDGVKVFEGLPSEINRAKFKEIYGEEAQDMRATALV"
     gene            complement(18005..19057)
                     /gene="phnD"
                     /locus_tag="P5516_06100"
     CDS             complement(18005..19057)
                     /gene="phnD"
                     /locus_tag="P5516_06100"
                     /inference="COORDINATES: protein motif:HMM:TIGR01098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0043168 - anion binding [Evidence IEA];
                     GO_function: GO:0043225 - ATPase-coupled inorganic anion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate/phosphite/phosphonate ABC transporter
                     substrate-binding protein"
                     /protein_id="HRT92046.1"
                     /translation="MKKAMFVLISVLVLSTLVLSACAQPTAAPTKAPVATEAPAATEP
                     PAPQPTEAPKELGTAENPIIIALAPSATAEQLTVGGEAIAAKMKELTGLTYSVIIPNS
                     YTALIEAMASGNAHIGFMPTLAYLLGKQAGAMDVKLVVVRNGSDFYGAQFIAHKDMGF
                     TPYFDPNTNTDTADAATALAQFEGKRPCFTDPLSVSGYVIPFGVLKEQGVNFKTPAAV
                     QSHPAVVQALYAGVTVSGDKKVGAICDFGATYIDARTSDKIHTDVNEKIVVIWRTDNI
                     IPNDNISFSSAMPEEIGAKVYDGFVAMAADEAGKEIFKNAGYEIEDLKPAEDSFYDAF
                     RSALQASGVDITTMVK"
     gene            19603..20496
                     /locus_tag="P5516_06105"
     CDS             19603..20496
                     /locus_tag="P5516_06105"
                     /inference="COORDINATES: protein motif:HMM:NF013134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HRT92047.1"
                     /translation="MINPHPGLPELEYVKPGTLAEACKFLEQHEGKARPFSGGTDCFV
                     RLRDGVLKLEYLVDIKGLDGTKELSFDPKKGLTIGAAITMNQVIAHKDVVKHYPNLVE
                     AAKSVASYQLRNRATIIGNICNASPAGDTIGTCLVLDGVLNVHGTGGKRTIPLNTFFL
                     APGRTVLKPGEVVTSITLPPPPAGMAGVYKKLGRNKMSDLSIVGVTVMGYPNSQSPSG
                     FSFRIALASVAPVPMEALKAEAILAEKKITAESIALAAEAAMEAVTPIDDVRGSARYR
                     KYMVRNLTREAITEVWNTLTK"
     gene            20529..20993
                     /locus_tag="P5516_06110"
     CDS             20529..20993
                     /locus_tag="P5516_06110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HRT92048.1"
                     /translation="MSYSKITLTVNDVVETVDVPHHMTLLQMLREKLSLTGTKNGCSA
                     GECGACTVLMNDEPVNSCMVLAAECDGARIVTVEGLAKSGKLSKLQEAIIKTGGVQCG
                     FCTPGMLISATALLKRNPNPTEKEIREALVGNLCRCTGYFRIVEAVKEAAHS"
     gene            21051..23408
                     /locus_tag="P5516_06115"
     CDS             21051..23408
                     /locus_tag="P5516_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HRT92049.1"
                     /translation="MANAKSTEKTTAVVGTSVIRNDVLEKVTGAATYVDDIQFGNKLL
                     YARAVRSPHPHALIKHIDTSKAEKLPGVKAVVTGKDFPERIGLYLKDKTIFATDRVRF
                     LGEPVAAVAAVSEEIAEKACSLVEVKYEPLEGVFDAEYGASEKAPVIHPDLGKYDCVN
                     FIFPKPGTNISNHFKIRKGDVEAAWKQCAHIVEKTFKVPHIQHVPIETHGAVAMMDEK
                     GKVTVWAASQSPFAQRNLIAKALHISHSDLRVIAPLVGGGFGSKAGLTMEAIPVALAT
                     KVKGYPVKFILTREEEFYTNFVRQGLTIHVKVGCDKDGNILGLKNVMYWDGGASTEYG
                     TNITRAGGYSSSGPYDIPNVWTDSICVYTNHPIGGAYRGFGMSELHTGIDQAIDELAE
                     VAGFDRVEFLKKNAISEGDIVVTGMTMHATGIQQCIDNVAKAIEFSKKEEPSSPDRLR
                     GKGIAIMWKAPAMPPNAGSSAWVELSEDGKATVGLGGQEIGQGTFTVMAQIAAETLGV
                     KYEDVRIAGPVDTQYSPYEWQTVASRLTWSMGNAVRLAALDARKQILDVVAEAWNETP
                     EDLDIVNGMVVSYKSEQETPLANLVIYGLPKPNDEGWMGGPIVGRGSFMPKYVTNLDA
                     ETGQGARAVVHYTVGCEGLDIEIEKNTGRIHVIKAAASFDVGKAINPALVKAQVEGGF
                     VQGLSSAMFEEIRLRNGIMTNPSFVDYRIATAADADFKLDTQYVEVPQDDGPWGARGV
                     GEHPMVPTIAALGNAIYDATGVRLQGPPYSAESIYLGMLNAGVVK"
     gene            23470..24711
                     /locus_tag="P5516_06120"
     CDS             23470..24711
                     /locus_tag="P5516_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HRT92050.1"
                     /translation="MTDHYDLIIRNATLRNEPKSTQDIAIQDGKVALIANKIEAKAAS
                     ELDARGNLVTESYVNTHLHMCKVYTLMMMDEAALKDYHGADMGKAMTAIELAARVKEK
                     YDEKWIIKNVRKAAALAARYGCTHIRAFADVDSKGKLEGIKALIKARDEFKGIVDIQT
                     VAFAQDGLVREPGADKLMRQAMDLGADVVGGIPWIEYTDADIAEHVRICYQLAKEYDK
                     PVSMLVDDAGDNGLRSLEVMALETIRTGWQGRALAHHARAMALYPKPYLQKLAAILKK
                     ADMGVVSDPHTGPLHARVRELLEEGVLVCLGQDDISDAYYPYGRNNMLEVAFLNSHLL
                     WFTTREDMEVLYDMITTKPARAIGLKDFELKKGAPANLVVLDQPNVLEALRYHEKPVY
                     VISHGKLIDQAKMDKIIQENS"
BASE COUNT         6030 a         6585 c         6317 g         5821 t
ORIGIN      
        1 gcttcacgta aaggcttctc ccaggggtcc aggccaacct caagcgcacg gttaaaagcc
       61 agagaggcga agtgcccatc accgcagccc aggtccagcg ctggggcagg caggtcaatg
      121 ttctcataga atctcgcttc caccgccctt aaaagagcgc gaaagtacgg caacgagctg
      181 atttgcagcc acaggtggtc tttaccggcc tggctggctt caacgggtgt gtgtcgctgc
      241 aaggtcacgc ttgctcctga aaaataacct catcattctt gaaacacggc gcttatgtat
      301 taccgcgcct attataaacg ttttgaaatg gaacgaaagt tcccgtcaac tcaatgagct
      361 ggcgggaacc agtatttggg cgcaaagata gaacaggtct gtaaattatt ggctcatttg
      421 ggcgagggct tttaagagag agttcgccac cagagtgcca gattgggtgc gataattttg
      481 cgtgcccatg cggcttccag ccattaaggc gctcaaaaga gcgttgatgt cgctgctgcc
      541 ggattgtttg gcctgcatgt aagccatgcc ggctttcagc agctgcgcgg tgtcgatctt
      601 aaccggtttt gaaccgcttg gtgcagcagc gcccgttaat ccgccgagga ggctgcttaa
      661 caggtcatca cctgtcccgg cgcctgcggg cgcctgttgc gcgccggcaa gtccgccgag
      721 cagcgagcct aagaggtcgc cgcctgcgcc agcgcctgaa ggagtctgct gcgcgccggc
      781 aagcccgccg agcagcgaac ccaacaggtc gccgcctgct gattgcccgg agggttgctg
      841 ttcaaaggtt tttctggctt gcgtttgcag tgtttgcggg gtgacctttt tctgggttcc
      901 cattaacgcc tgaagcaggg tcagcgcgtt gttggtggtg agagtcttct ttcctcccag
      961 ctgagcagag gcgtcttgca acccctgagc ataaagtttt gctgaactgc tctgactctt
     1021 ggcctgcagc agctggctgg cgtaagccag ttgttcagcc ggtgtggcgt tgctcttcgc
     1081 tttcatcgcc tgggtgatta cctcgaagat ctccaccatg ttatcgccgt gattgtggtt
     1141 gtaggtatcc gcctggttta aagccgcctt atgctggtcc aatgtggaaa ccacgctgcc
     1201 aaaaagttgc gccagatcaa tactctggtt tgtcatgaga atttcctttc cctgtttgtt
     1261 tttgtctgca cattatatcc tggttttgtt gacaaacgaa attcagtatg ttatgattta
     1321 cctattcaaa tattgaatat tcatcctatg aacgataaaa aactcccact caacgcctct
     1381 gaatacgccc tgcttggttt cctgaaggct ggaccgtgcc acgggtacga gctgcaccga
     1441 aagctcacag aaccagacgg cgcggggatg atctggggaa tcaagatttc aaatatgtac
     1501 gcccaactgg ataaattgga gcggcgcggg ttgatcagcg gtcgtttgtt ggaaaacgaa
     1561 cagcgcccag cgagaacgca gtactcgctc accgctgccg gaaaacaact gttcgaagag
     1621 tggctgcatc aaccagtgca gcacccgcgt gatttccgcc actttttttt tctgcaatta
     1681 ttctttctct ctcagtacga gcctgacgcg cttgaatctc tggtggagcg ccagctggag
     1741 ctctgccagg cgtggcagca aaatttgttg gcagcgcagg agaggtcttc cacctccgtt
     1801 gatttcgctg acctgaccaa tcgttttcgc cactcacaga tccaatccat gatcgattgg
     1861 ttgttctgga tcaagagtga aaaaatcaaa atcaaaccaa agcgaggtaa tcaatgaaac
     1921 gtccccttca attcttagtg gtacttgttc tgttcagcag tttactggca gcatgcgcgg
     1981 cgcccacgcc cgccgccgca ccttctctca ctgaaccccc agccacagaa gctccggcag
     2041 ccgtgagcac gccagcccca accgagccac cggagatgga aaaaatcacg gtcgtagacg
     2101 cgcttggacg ggaagttgta tttgaccaac ccccctcgcg catcatgatc gtgggcaagg
     2161 cagcgttcat gttgcttgac gcagctttcc tcttccctga ggctcctgaa aaaatcatcg
     2221 ggtatgagct gcgttctcaa accggactgg atttcatcca cacggctttt cctcaaagcc
     2281 ataacatgat cgtgcttgag atggacgctg gaccggaaca gatcgccccg gcaaaccccg
     2341 acctggtatt gatgaagtca taccttaaag aacaactggg agaccaggtt gagctgcttg
     2401 gcatcaaggt ggtgtacctt gatctggaaa cgcccgaaac gatcaaccag gatatccgca
     2461 cccttggaaa actcttcggt aacactgagc gcgctgagca attggtgacc ctctatgacc
     2521 aggcgcgcgc gaaaatcgcc gatatcacca acgtcatccc tgaagaagaa aaaccttcca
     2581 ccctgcttct ccaatattcc gataaaggtg gcgaagtcgc ttttagtgtt cctacaggcg
     2641 gcttcttgca aacacaattg gtcgaaatgg ccggcggggc gcctgtctgg aaggaagtga
     2701 cgggaaaggg ctggtctgtc attacactgg agcaaatcgc cgcctggaac ccggagatca
     2761 tcatcgtcgt tgattaccgc ggaaaagcca tggaggccgt tgaccggctg aaagcagatg
     2821 aaaactggca gcaactggac gcggtaaaga atgagaaact ctacgccttt ccattggact
     2881 ttcagagctg ggatcaacca gattcacgct ggaccttggg cctgacctgg ctcgcaacca
     2941 ttatgcagcc agagcgtttt gcatccgtga aaatgcacga agaaattccg gccttctaca
     3001 cggatttcta cgggttaagc gaatctgtga tcgagcagag cattctgccg ttggtgaagg
     3061 gcagtttttt agagtaagcg ggtaataaaa aatcacccgg tttgtgcagg cagcattgaa
     3121 aaacaacaaa aacctcaccg ccttcgtgct cgtggttacg ccgctgattg ttcttggtat
     3181 ctctttattc atcggcagat acccaaaacc gctcttgatg cccctgtctg tgttgctgca
     3241 cgaccagctg gggcaaaacc tggtttacaa tctgaggtta ccacgcctga tcacctccgc
     3301 cctgctgggc atgtcactgg ccaccgccgg aggtgtgatg caaatgcttt tccgtaaccc
     3361 gttggtagaa ccggggttcc tgggggttac tcagggggca gggtttggcg ccgccttgag
     3421 catcctcttc cttacaaccg cgccgctggt ggtagaactg agcgccgctg gtttcgccat
     3481 catcggcttg ctgctctctt atttcatcgc ccggcgaatc cgttttggcg ggtgggtgct
     3541 gcggcttgtg ctctcaggta tcgcggtttc agccatcttt tcagcggggc ttggtatcct
     3601 taaatatatg gcagacccca cctctcagct gccagagatc gtgttctggc tgctgggagg
     3661 tttgtggtct gtcacctgga cggatctatg gtatatcctg cctgtggtgg ttatcggtct
     3721 ggtcattatc tacctcatgc gctggcgctt gaacctgctt tcgttgagcg acgagaccgc
     3781 tttttcgtta ggagtggcgg tagggcgcga acgcaccttg ctgctggcag ccagcgtggc
     3841 gattaccgct gtggtggttt ccgtagcggg catcgtgggt tggatcggtt tgatcatccc
     3901 ccacattgcc aggcgcatcg ccggtgccaa cgcggcctcc ttcataccgg tgagcatgtc
     3961 attaggcgca gtctttggca tcatctgcga tgacctggcc agggtgctga ccaccggcga
     4021 agtgccgctg ggaattatca cctcgctcat cggcgctgtc atcttcttga tcatgatgac
     4081 ttctcgttcc atgaggatcg caagatgaac ccagcggtga tccaggtcaa cgatctgtcc
     4141 ttttcgtaca acccggcagg caacgcggct gttcaccagg tttcgtttga aatcctcccc
     4201 caaacggtta ccgctatcct ggggcccaat ggcgcaggaa aaaccacttt attgcacctg
     4261 ctcttgcggc ttaaaactcc gctcacaggc tccatccacc tgcaaggcag accactcaat
     4321 cattattccc gtcatgaact cagccagaat atcggcttgg tgccgcaaac agagtacatc
     4381 ccatttgaat ataccgccct ggagtatgta acgctcggca gggctcctta cctgggcccg
     4441 ctagaaattc ccgctgaaaa agatactgaa atcgcccgtc aatccttgaa agaggtaggg
     4501 gtgtcgcatc ttgaaaaccg ccccatcccc gccctgagcg gtggtgaact gcaactggtg
     4561 ttgctcgccc ggtctctatg ccagcagccc cgcgtgctat tgctggatga acccacctct
     4621 cacctggatc tcgccaatcg aaatatcacc ctgcaaataa tggaccgctt gcgcagggca
     4681 ggcacgaccc tggtcttcag cacgcatgac cccgaagccg ctgccttgat tgccgacaag
     4741 ctggttttaa tgcgcgccgg gcaagtcctg gaaaccgggt cgttggaaga ggtgttcacc
     4801 acagaaacgc tgagccgcac atacggtacg cgggtggagg tgctccaggt ggatgggttg
     4861 cgtgttgtaa aatccattga acgtcttggt ggagaacaga atggccaaaa taatcctgct
     4921 gagcgctgaa aaaggcagcg gtaaaaccac cgcgctcctt aaactgaggg agaaacttac
     4981 cggcctgacc ttaaactcac gcggggtgct gagccccccg gtgatggtta acggggagaa
     5041 gatcgccatc gaattaatgg atgcccggag cgctgaacgc aagcgactgg cagtgccagc
     5101 cgcgcttgat tctccagaag catctggact aagatggcat tttttcgaag atacgctgga
     5161 ttggggaaat caccttcttt cagaggcggt tccctgcgac atcctttttg ttgacgaaat
     5221 gggcccgctc gagttcgacc gaaacgaggg gctgaccaag gggttcttcg ccatcgactc
     5281 gcggcgtttt ctggtggcgc tggtgaccat tcgccccgcg ctgctggaaa gagcccttgc
     5341 gcgctggtca gatgcccagg tctgtaaggt gactattaag gaccaacctc acctggtcaa
     5401 caacctgttc cgcctgatca ctggttaacc gtattttgcc ccagccgcag tttgaaattt
     5461 ttacgccaat cccacgtata caatattgat atgaacccaa tcagcgagtt gttgagtaag
     5521 gtaaaaacct tgtttccgaa aaaggtcatc ggcctggcct tgagcggcgg cgccaccttt
     5581 ggcgctgcgc acgtgggcgt gctgcaagta cttgaagaaa acggaattcg cccgcacctg
     5641 gtcgcaggta ccagtgccgg ggcgctgatc ggcgcggctt actgtgcggg tatcccgttg
     5701 agcgagattg aaaccctctt tcgcacgatg aactggccga cattgctccg cctttccatc
     5761 cggaattcac tctctatttt cgatacacag cccatggaag aatttctgcg caagaagatt
     5821 ggtgatatcg aatttcaaga actcaggatc ccgttcgccg cggtcgcctg tgacattcac
     5881 accggcgcaa gggtcatctt agaccgtggc gcgctcgcgc ccgccatccg tgccagcgct
     5941 gccattccag gcttgttttc accagttgag gtcaatggcc gtctgctggt agatggggga
     6001 atcgtggata atttaccagt tgaacaggtg agagacatgg gcgcgagata cgtgatttca
     6061 agcgatgtat ctcaccgtgg accaaactcc aagaaaccgg aaaatctctt cgaaatcatg
     6121 ctgtcgatga tctacattat gcaatctcgc gccgccctcc cgcttgaaga cgcatcggat
     6181 tgttacattc gtccccaggt gtctcaatac tcatcctggg gcttcaagga tgtcccgcaa
     6241 gttatggagg ctggaagaaa agccgccagg ctggttatcc ctcaactgcg ccggcagttg
     6301 cgtatccgct aacacgcgcg ccggataaag aaaatggatg gcccaagcct acggccatcc
     6361 atttcagatt tcccccttta gcgaagaaca ggtatgcctt gcaaaggcaa taaagaaatt
     6421 actccagact gtctttctta ttaaataatt gaatgaataa ttgcaccact tcacggtcat
     6481 aggcgcttcc tgcctgttca agcagttcct gcagtgcctc ttgatgcttg cgcttctttt
     6541 gatatggccg tccatccgtc atggttgtat aagcgtcagt cacagccagt atacgcgccc
     6601 ccaaagggat ctcgtcacca cgcagaccat tggggtaacc ctttccatcc atgcgctcgt
     6661 ggtgagcgag gatgagcggc gctatctttt caagccctgt caccccgcgg acaatttgcg
     6721 cgccaatttc aggatgggtc ttaatgatcg cccattcttc ttcggtgagg ggtcctttct
     6781 ttttcaaaat tgtgtctttc acgccaattt ttccaatatc gtgcagcaac gctgcccagc
     6841 acaattctct tgtctcacgg gcagaaaaac caaactcttt ggccagctgc tcagaaagag
     6901 ctgccatgcg cttgctatga gacgcgctat gcagatctcg ggtttcaagc gcctttgata
     6961 aaacgatgac ggtctcaata gatacagact cgatgcgctc attcatttgg gcgcgaaaga
     7021 ccgcgctcgc cagctggtcg gcaatcagat ccaccaggta ataagaatca ggcgtcagta
     7081 tctgttcttg ctggctggca gactgcccta aaatgagcac accgattggg ttcgtttcta
     7141 cccgcatggg aataatccag gatatatcct ggttggatat tcccaggctc tttcgttctc
     7201 tttccgataa gtgttccatc cccaggaaca gcctggtatt gttttcagca tccaaaaacc
     7261 tatggcagat gttttctatc tcaatcgggt ccagagaggc tttctttttg atcaacattt
     7321 cacgtgcgga ataacgcgcg cggcaattac attgcccgtc aaggtccatt aaaagaatct
     7381 ggcaaaaatc cacttccagg atctcaacga tacgcctgga agcgcttact gccagttctt
     7441 ccagattctc tgaacccgcc aggaagcggt tcagttctga aagaccattc catttttctt
     7501 gtagataagt tgggaccccc ctctccctgc gtctgtcata ccgcgcaaat gcatcaattg
     7561 tctgtgccat gtctcttacc ctgttgatgg ggagtcaata atgacgaacg atcgtttcgt
     7621 tcgtagaaaa ttagttggtt gtctatctac tccgctaaat tgagcgaggg ccgggtcatt
     7681 tctgcgttca tctggcgggc gaactcagcc agggtggaaa cgcggatggc agaaatcctg
     7741 cgggtttctt cggcgccaag gtgaaacgcc tcaccggcaa aacccttctt aatcgccatg
     7801 ttggggtttg ttaatagcaa tctgcggaat ttttcattca caactgctgc agtgagaatg
     7861 cggctgtatt cgcgttcttt ttgcacaacg gtttcatcgt gatgatggta atcgtgaaat
     7921 atgttactca ttttattctc ctatacattt acactctttg gtccacccag cgaccagagc
     7981 agccagacga ttcccacaaa gaggatgata tcgcctgggc tgaacgccac ctggtagttg
     8041 atccaatcgg gtaaagagaa gcggtcgctc agaaaccaca agcgggtgtt ctcaggcagc
     8101 aagacgatat ctttgctgta gccgaggcgc tcgcccacca tccagtcgcc tgctgttgaa
     8161 tctggcagca gccgttgcac catctctggg cttatcggca tccagccgcc attgagcaag
     8221 atgaccagcg cgttcagcaa aaaaccgccg gtgattggcc aaaaactggg tttacggata
     8281 ttaaagagcg aaaatatgat cagtacaacc agtgaaatga tgaaaaggaa ggaagccgtt
     8341 tcatcgggga tgcgggttcg ggtagatggc aaaaagaaaa tgaagccctg gggaatgaaa
     8401 gccagtaaga ccaatccagc cgctttaagg tcatagcccc tgtattccct tttaccatat
     8461 ttcgccctgc acagcccggc ggctaaacca accagtactg cgacgaccag gatcatttat
     8521 tgtcggctat ccgccaacag tgccggtggc gaaaggagcg cccgctgcca gggtgaacag
     8581 taccagcacg agcacgaaga agacgaacct tgctttgttg gaaatggttt tcatgagaga
     8641 ctccttnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     8701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnngcac gaagaagacg
     8761 aaccttgctt tgttggaaat ggttttcatg agagactcct tttttactct tatcattttg
     8821 tcaggacggc gctaaaagca cacaggcttg cagcagccag gacgattgag atggatctat
     8881 ccgccaacag tgccggtggc gaaaggagcg cccgctgcca gggtgaacag taccagcacg
     8941 agcacgaaga agacgaacct tgctttgttg gaaatggttt tcatgagaga ctccttaaat
     9001 gattagtcgt tttttttact ttacctgatg attatgcctg caggcaggat gcaaaacagg
     9061 atgcataatc gactcttaac ttcagggagc tatcctgact tgttaaacac caaaattcag
     9121 agatggtctt atattcacct ctttctcatt tgatcttcaa tttggctgga taaatgctta
     9181 ctgttgcatt aatgaatgga ataacttgac ggtctgtggc gagggttcaa tgtcaagttc
     9241 ttttttcaat acaagcttgc acctttcaaa ttgcctggcc accgcagaac gatcattcat
     9301 ggcagagtag gccaacatcg cagaacggta cgcggattcg ttgtaattat cttcctcaag
     9361 cgccctgttg gcaagggtgg ctgcctgtga atacttgcca actttcatat agagattgat
     9421 gagcgcctgc gctgcgttca agaaggttgt gtgaaatttt tcgcgcgcga tgagcaccca
     9481 ttcctggtct aatttgggca agaaaggacc gtgatagcaa gagaccgccg cgcgcaaatg
     9541 ttcgattttt tcttccgtgc tttgcgcctt tttggcgttt tccagctcat tcataaacgt
     9601 atccaaatca tattcataat ccattgagcg attgaaccgg tagatatcat catcaaaggc
     9661 aatgcaatcg ctgcccgctg cccggcgcaa ccggtagatc gagttcttaa accgcagttt
     9721 caccgtatca cggtctgcgt ctggccaaaa tgcggcccca acctcttctt tggtcgctcc
     9781 ctcagggtga gaaaggagga agaaaaacaa atcgcgtact gtatgtgttt tccaatcctt
     9841 gttggtaaca acccggttcc ccagccgcac ctgcatgcgt cccaaaccac tgatgcttaa
     9901 cttggggttg gagaaatcca ccgctgagga atgccggcgc accacacgat gtatttgcgc
     9961 taaatgtttt tcaaaagccg ccacttctga ctttaattgc gttaccacag ggtactgttc
    10021 atcctccggg ttgatgagct ccagatagct gccaaattcc atcagtattt ggattaaaac
    10081 ctggcgcatg ctcccttctg atttttgcat ttcagggtct ataacaagct ttgtgtgtgt
    10141 tctggctgtt tcaaccaaag acgcgaggtt acggtacacc gccgccttaa atctctccat
    10201 ttggtagcct gccgcggtga agtaatcttc cagttgttcg aaagtatgcg ccaaatccgc
    10261 tggtttttgg ttcttaaaat ccagtatcgc ctgttccagc aggcagagat ggtgttcata
    10321 cttcgagccg cttttttgcg ctttttctaa tgccgctgat atttgttcct gggcggaaga
    10381 aagatagccg gttatgcggt caagcgtcgc cagcgccagt gtgaggtaaa catccaaaga
    10441 aaggtcgctg gttttctgca acacctcttc tgcctggcga taggcctgcc tggcttcttc
    10501 aagcgcgcgc acatcacgat acagatcacc caggctggtc agtacatacc cttcgatcct
    10561 ggggctggcc aacctggaat aagatagcga gcgttccagg gtaatgacgg cctgttcaaa
    10621 ctggttgcgc aggtactgga tcatgccgat gttgtttaag acattggcca gccatagagg
    10681 gttttgcacc gcctgccagt attccagtga agtggagtaa catttttcgc tttgctcaaa
    10741 atccgctcgg gcggaataaa tcaccccaag atccatcaat actttcgcgg cgtccgattc
    10801 cagtcccagg tcttgaaaac ggcggtaaga ctcgcgtaat ttccactgcg catcattcaa
    10861 ttcaccggtt tgcaaataca ccaacccttc cgctctcaat gcctcggcgt atttttgagg
    10921 agcgccaaag ggtggtctgg tcaattcaat cgcgtcctgg gcgtctttaa gcgcgttttc
    10981 ataatgcccg gtatggcggt tgacagcgct gcgccggatc agcgcggcag ctaattcttc
    11041 tgcgaagccc actgcgcgca actccttgat cgcccggctc agcaattcca ggctgacctc
    11101 ataatcacct cgcagcatgg caatcgttcc cctgatagac accaactccg gcttattttc
    11161 caccaggtca aagggaagcg cgttcaacca atccgtcagc gttaccaacc gcccgctgag
    11221 gatcatcgct ggagcagctt tgcgcaccat ttcaacccgt tgttccgggg ttccattcct
    11281 cagcaaaatg gcaatcgctc tttcccattc ctgcacactt tcgtaataag ccaccagttc
    11341 agtttcgatg cggagtgttt cctccggctt ctctacgcgc atgcggttct gcaggaagtc
    11401 gcggaagagg tgatggtaac gcaggaaaag cgtttcatcc cctaccggca gcacaaacag
    11461 gttgtcgcgc tggattttct caatgacctc gtcccagttt tgcccctcta accccagcgc
    11521 tttacccacg attttttcac aaaggttggc atcaaattct tccagcaggg atgtgcgcaa
    11581 tagaaagctt tgcatggcca gcggctgccg gtcaaagacc tgctgggtaa gatactcata
    11641 gatacccacg cccgacacct tctctatgcg gctgcgttcg taaggtcctt taggcgaaac
    11701 ctgcgcggtc aacagcaggc cggtgatcca gccttcagta cgcgtgatca tttcctcggc
    11761 tttttggtcc gaaatggact gatgataatt caccgccatc aactgcttga tctcttcagg
    11821 cagaaaagcc agttcttcaa agctcaagcc atccacctca gagcgcgcca ccagcaggct
    11881 caagtctggc agagtgagca gcgtgcgaga ggcgatgaca taatggcaat tctccgctga
    11941 ctcttgaatg atgcggttga taaacacttc gataggttta acatcacgaa ccaggtggta
    12001 atcgtccaga acgaagataa agtgctcgct gatgttttca taggcgtcat tgacgatcgc
    12061 tgaaatcacc aggtccaggt tcaaattatc ctggctcaat tcagcgagcg ctgctgaagc
    12121 catctgcccg aaggcgggga acttcccctg gatggcagcg ataaaatgcg cgatgaagcg
    12181 cttggggtcg ctgtccagtg aatcgagcgc gtaccagcag actggtattt gagtgtgatg
    12241 tgaaaaatca accagcagcg atgttttacc ataaccagcc ggagcggcga caattaagag
    12301 cttaaggtcc agcaaattat caagcgcggc gagcagcctg gggcgcgaga ggatttcgct
    12361 gcggcgtcga ggtataagcg tttttgtgcg gatgaccgga agatctggag gcatatgtat
    12421 ctactataat ccagtctttg tcatgctggg cttaacgaat atgtctactt gaattatata
    12481 caaaataaga gtcctcttcg gactcttatt ttgtacgatt cgagaaatat taatttggct
    12541 acattggtat tggtgggttt atcggagggg tttacacggc cggttggccg gcgattgggc
    12601 ccaggtactt ggcatcacca aaggctaaaa tcgccatgaa aatgacaccc aagaagagca
    12661 agccaaaccc aaaacccgtg ctttttccaa atactttagc caggtcgaaa atcacgataa
    12721 tcccaatcac aatattcacc aggggtatga ataagagcaa aagccaccac cagggacggc
    12781 cgatgatttc caattcagtt attatgttat agattggaat gatcgcagcc caacctggtt
    12841 ttccagcttt tacaaatact ttccacaaac acacaatgga aaaaacgctg acaagcaaaa
    12901 gaaagagata gtacccaaag cctaaaccac catcatagtt gtaatccata cgtcctcctt
    12961 tttaggttat gattacgttg aattaatcat aacataattg tcaatatatt ggatatatat
    13021 tatccatttt gaaagggcaa aatgagcatc ttcttatcca agttccttcc cctcttcctg
    13081 tacccagcgg ggctggtcac gctgcttata ctcctgaccc ttgttttctg gaagaaacgc
    13141 cgggcggcct tcacatttct tgtcctggct ttcttcattc tgctgctcgc cggcaacaaa
    13201 tacgtcgcca cagccttcgc gcggacttta gaatggcgtt acccgccgct ggtggaaaac
    13261 accactgccg atgtgatcgt tgttctgggc ggcggcactg aaccagcaat agaaccgcgc
    13321 cgcatggtgg agttaaacgc cgccgccgac cgggtgcttt acggtatcaa gctgtaccag
    13381 gaaaaagcag caccaatgct cttgctcagc ggtggagaca ttgactttat cggcgattct
    13441 ccctccactc ccgctgatga catggcaacg ttgatggaga tgctcggcat cccgcgagag
    13501 aaaatgatta tccagaacgc ctcgctcaat acacagcaag acgctgagtt cagctgcgag
    13561 cttattaaaa aaaatggctt taaaaaagtc atcctggtga cctcggcgtt tcatatgccg
    13621 cgctcggtcg ccctttttga aaaacaaggc tgtccggtcg tcccagcacc ggtagatttc
    13681 agcatcaccc agctaagttg ggataacctc acccacccaa ccactgctga attcatcctc
    13741 gacttgctgc cttcgtactc gcatatttcc acggtgacca agactttaaa ggaatacttt
    13801 ggcatatggt actataagct ttccggggta ttgtaaacag cccgggttga aagaaggaga
    13861 agataatgaa acctaagttc atcgcgcttg cgcttatcag tctactcctg gtggcttgtt
    13921 cgctgccaaa atcattaact tccattattc agcgatttag atctcaacca gcagcggtgg
    13981 cagaatccaa ttccttgctt aacctggttg aaaacgggaa agtgagtgtg gtcggcatcc
    14041 acggtaacgt cggactgggc accttcactg gcaggacgat cgacttacag ctccaaaaca
    14101 acaccgacag caaactcgat gtgctcatcc cttgtgggct ggtattcatc gctgatgacg
    14161 agaaaagcag ccgcatgatg gttgttcaac ccaccgtgac cagtctgaac aaagggaaga
    14221 cagcggttgt aaagccgttc gtcctcagca ttgacgccct aaaaggttta ccctcgcccg
    14281 aaaagacata cagtgtcgct gaactggaag aagggaaaaa caaagatttc gccacctgtt
    14341 tatgcgaaca ggacctgccc gcggaaaccg agaccaaaga ccttctcgac cttcagctcg
    14401 ccgcctggat ggtcagcggt gaagggcttc tgtccaatat cccggtggac ctcaacgacc
    14461 tgttcaaaga cttctccggg ctgcccatca ccatccctgg gctggatgaa gtgatgaagg
    14521 atgtggccgg gaatgtcgcg cccaatgcgc aagcctggct ggataaatgc ggaatatcct
    14581 ttggcaagtg attcacaaga ggctggtcgg taaccctgac cagcctcttt gatattcatt
    14641 taaagaaggt ctataccagc tgtttgcgta tctcagagct cagaaagtcc agcgcccaca
    14701 ccaccaagat gatcgccaac agggctgtgc cagattggga atactgcagc gtgttgatgc
    14761 gctgcgagag gaaatatccg attccacccc ccccgacaaa accgatgatg gtagagatgc
    14821 gcacattgat atcccaatgg taaatggtaa aagaaagaaa gtccgggatg atctgcggca
    14881 ccacggcata taccaccacc tgcagtttgt ttgcgccggt cgcggtgacc gcttcgatag
    14941 ggccagagtc gatgccttcc actgattctg aaaacatctt tcccagagaa gcggctgtcg
    15001 taatggaaag cgcgatgaca ccagcgaacg ggctgccaaa ccccacgatc atggcaaaga
    15061 tggtggccaa caccaacggc tcgtatgaac gtatgatatt caagaaagcc ctgacaatat
    15121 agtaaacagc cgagccaagc catcccttgc gggtgatatt ggaggctgcc aagaaactga
    15181 gcggcacggc aaagatggtg ccgatggtag tggcgatcaa ggccataaag atggtcacaa
    15241 acatcgcgtc cagtacatcc tttaaggtct gagatgccac aaccttgcct tccgggaggg
    15301 taatttcaaa cgccagttgg gcgggcttcc cgtcaaccgc cgctgtgatc ggtcgcaatt
    15361 ccattgtttc tgtcaattca ccattggcat tcgtcaccgc gttcttaata cttaacagcc
    15421 cgccatccgg caatatccag cgaatggaca gttcagcgtt cttaggcagt tcatatcccc
    15481 ttacctgaac tttgtccttg gggttaccgc agggcggatc gacggtgatt cgcggcccgc
    15541 tggccggcac ttcagggttc gcgacgctgc cgcagggtac cggcaccagc tgtgccacgg
    15601 tgattttttc cgattcgcgc cccaggatct caggtcttaa caactgaccc ataatcgatt
    15661 tcgccttggg gaaagaagaa accagcttaa ccaggtcggg tttggtcaca cggatactga
    15721 gcaccagcag cgcgatcacg gcgatgatga tgagcacccg tttgagtgaa tgcagtaacg
    15781 atttcagcgt ctccatgtca caccagccgt tccctgatac gggcgctaaa gaagtccagg
    15841 atgatcacaa ccaccgcgat ggccacaaac gcggtgccca cagcgcgata atcctgcagc
    15901 acgatccact gccacaggaa gaacccaata ccgccgccac cgacaaaacc gatgatcgtg
    15961 gaagaacgga tattgatatc ccatcggtaa atggtcaacg cggtaaatgt cggcaccacc
    16021 tgaggaataa ccgcgtagcg taccacctgc ggccagctgg ctccggttgc cttcaccgca
    16081 tcgatcggtc cgctgtcaat cccttcaatt acctctgagt agagcttgcc cagcgccgcg
    16141 acggtgtgaa tggtgatggc aatcatgccc ggaaaaggtc caagacccac gataatgaca
    16201 aagatgatgg cgataatcag agactcgatc gaacgcacga tattgaggat tgtcctggtg
    16261 accaggaaga tcgccgtggt cactgggttg ccggtcatca agttgcgggc tgccaggaag
    16321 ccaaacggaa tggcaatgag cgcgccgaca atggtggaca gaaaagccag cgccagcgtc
    16381 tcgtaaatac cctgcacgac atagccgccc acgtctgaaa gctcgatacc gccaatgggg
    16441 cgagactgtg agactttgat gcgatgaatc tgcggcactg tggggtccgg cgcggtgctg
    16501 tgaacatagg gagagaccac ccattccagg ctcacattcc cctgcgcatc tgatttaacc
    16561 tgcgcgtcca tcgctttacc gccaataggg ttctgccagt aaataagagt gggcgtgttc
    16621 ggccagaaac cttcgccttt gaccgtgatc gtttggttca cagtggcgca aggttcaacc
    16681 acgtacaccc gcaccccgtt caccgtcctg tctggaacag gaggcgtggc tgaacagggt
    16741 gactgaatat cggtaaaccc ttcctttttt tcggttttgg gctgggtaaa atccagctgg
    16801 aacatagcgc tgacgatatc cttcgaacgt tttgagtttt cgaccagcgc gcgtaaatta
    16861 aactcggtta cctgcccgcc aattccgtac gccatgagta gccacaggag tatcgggaga
    16921 acagcgttct ttcccccggg cagcgaagat gcgtcccaaa tgttccacaa ccatataagc
    16981 gcgggcagaa tgtaccaggc gggtttacca taccacgcca caacagcaat cgaagtggca
    17041 accgccagga aaatggcgat cgcagcacga tattttttct ggatgagttg tcccagtcca
    17101 ggtaccaggg cagaaagaaa aatcgagacc aatctggagc gagatgtttt ttctttcatg
    17161 agtgtcaaac aagagcggtg gcgcgcatat cctgcgcttc ttcgccataa atctctttga
    17221 acttagcgcg gttgatttca gatggcaatc cctcaaaaac cttgacccca tctttcaagc
    17281 cgatcacccg ttccgcgtag cgctgaatga gatccagata atggaggctg caaataacac
    17341 ttacattgtc ttcttcattt aacttttcga tatattgcat gatcgaatgc gccagcaccg
    17401 ggtcaaggct ggcaaccggt tcatccgcca ggatcagctt gggttcctgc atgagcgtac
    17461 gggcaatgcc aacgcgctgc tgctgccctc ctgaaagcgc gtcagcccgg ctgtgaactt
    17521 tatccgcaat accgacccgc gtcatcgcgg caagcgcgcg ctccttctct tcatggggga
    17581 aatatcccag tgtggagctc caggagttta gataacccaa cctgccgctt aacacgttgg
    17641 tcataaccga agagcgtttc accaggttga aatgttgaaa gatcatacca atttgccgtc
    17701 ggatatgacg caactcagcc cctttggcgg cggtaatatc aatcccgtcc atggtgatct
    17761 tcccttctgt ggggtcaatc aaccggttga tacagcgcag cagggtggat ttacctgaac
    17821 cggatagtcc aatgatgacc agaaattcgc catccggcac ttcaaaactg aggtctttga
    17881 gtgccaccgt accattggga taaaccttcg tcaaatgttc gactttaagc acggttaatt
    17941 cctttttaac tatttagggg aaaaactgga gggggtaaac ccccctccag cgaaacagaa
    18001 gaacttattt caccatggtg gtgatatcca cacctgaagc ctgcagagcg gagcggaaag
    18061 cgtcatagaa cgaatcttca gcgggtttga gatcctcgat ttcatagcca gcatttttga
    18121 agatctcttt accagcctca tcagcggcca tggcaacaaa gccatcatag actttggcgc
    18181 cgatctcttc tggcatggca gaggagaagg agatattgtc attgggaatg atgttgtcag
    18241 ttctccagat gaccacgatc ttctcgttga catccgtatg aatcttatca ctggtgcggg
    18301 cgtcaatata ggtggcaccg aagtcgcaga tagcgccaac ttttttatca ccagaaacgg
    18361 tcacgccagc gtaaagggcc tgcaccaccg cggggtgaga ttgcacggct gcgggggtct
    18421 taaaattaac cccttgttct ttgagaacac caaagggaat cacataaccg gatactgaca
    18481 gaggatcggt aaaacatggg cgtttgcctt cgaactgggc gagcgcggtg gcggcgtcag
    18541 cggtatcggt attggtgttg gggtcaaagt acggggtgaa gcccatatct ttgtgggcga
    18601 tgaattgggc accgtagaaa tcagagccat tgcgaacaac gaccagcttg acgtccattg
    18661 cgcccgcttg cttgcccagc aggtatgcca aggtgggcat aaagccaata tgggcgttgc
    18721 cggaagccat ggcttcaatg agggcggtgt agctgttggg aataatcacc gaataggtca
    18781 gtccggtgag ttctttcatc ttcgctgcga tcgcctcacc gccaacggtc aactgttcag
    18841 cggtggcaga aggcgccagg gcgatgatga tggggttttc cgcagttccc agttcctttg
    18901 gcgcttcagt cggttggggg gccgggggtt cggtcgctgc tggtgcttcg gtcgccacgg
    18961 gtgcttttgt gggagccgcg gtcggctggg cgcaggcact cagaacaagg gtgctcaaca
    19021 ccagcactga aatgagaaca aacattgctt ttttcacttc tctcctcctt ggattaaacg
    19081 tacaggtttg gcgtctttga taaaaacgca atatcaatca tataccacat atttttaaaa
    19141 ggcaagcaac tagccttggg ttcaacctta atattttccc gttcataagc catcgcaata
    19201 gaaccccgca caataccaag agagaaattc ggactgtagt cagttttttt ggtgcaattt
    19261 tccaggtcct gcagcgcaaa cctatattac atttatcctc atttgtccga caaggacaac
    19321 tttttccttg tattcatttt tttttcatgc taatatcaat atggcaaaga tcctcaattg
    19381 tttggtagta aaaaataaac tgcgctcttt tatgcgtcag gtttttgctg ttcaaaatta
    19441 cggttgccaa gaaagcgagg tgaaaaaagc ccgataagaa gaattcagaa atcgattcct
    19501 tcaaatgtag ttctattctc aacagcggtc aaaaaaccgt gcaacacagg gtgagaaaaa
    19561 gccctcaggc ttgaatctaa aaacaaaaag ataggacata ctatgatcaa tcctcatcca
    19621 ggacttccag aactagagta tgtaaaaccc ggtaccctcg ccgaggcgtg taagttctta
    19681 gagcaacacg aagggaaagc ccgtcctttc tccggcggaa cagattgttt cgtccgcctg
    19741 cgcgatggtg tactgaaact agagtatctc gttgacataa aaggtctcga cggtacaaaa
    19801 gagctttcgt ttgaccccaa aaaagggtta acgatcgggg ccgctattac aatgaaccag
    19861 gttatcgccc acaaagacgt ggtgaagcat tatcctaacc tggttgaggc ggcaaaatcg
    19921 gtggcaagtt accagttgcg caaccgcgcc accatcatcg gtaacatctg caacgcctcg
    19981 cctgccggcg ataccatcgg cacctgcctg gtgttggatg gcgtcctcaa cgtgcacggc
    20041 acaggcggaa aacgcactat tccattaaac actttcttcc tggctccagg acgcaccgtt
    20101 ttgaaaccgg gcgaagtggt cacctccatt acccttcctc ctccaccagc cggcatggcg
    20161 ggtgtttaca agaagttggg caggaataaa atgagcgatc tctccatcgt cggtgttacc
    20221 gtcatggggt atcccaacag tcaatcacca tcaggcttct ctttccgtat cgctctggca
    20281 tcggtcgccc cggttcctat ggaagcattg aaagcggaag ccattctcgc tgagaaaaag
    20341 atcacagccg aatcgatcgc gctagctgct gaagcggcga tggaagccgt caccccaatt
    20401 gatgatgtgc gcggctcagc ccgttatcgc aagtacatgg tacgcaacct cacacgcgaa
    20461 gcgatcacgg aagtgtggaa cacattaacc aaatagctcc ggctgtttga cctttacagg
    20521 aggaatgaat gtcttattca aaaatcacac tcaccgttaa tgatgtcgtc gaaacggttg
    20581 atgtccccca tcacatgacc ctgctgcaaa tgctgcgcga aaaactctcc ctcaccggta
    20641 ccaagaacgg ttgttcggct ggtgagtgcg gagcctgcac tgttctgatg aatgatgaac
    20701 ctgtcaatag ttgtatggtt ttggctgcag aatgcgatgg tgcgcgcatc gtcactgttg
    20761 aagggctggc aaaaagtggt aaattaagca agttacagga agccatcatt aagaccggcg
    20821 gcgtgcagtg cggtttttgc acccccggca tgcttatttc agccactgct ttgctcaaac
    20881 gcaacccaaa cccaaccgaa aaagaaatca gggaagcgct ggttggcaac ctgtgccgat
    20941 gcacgggtta tttcagaatt gtcgaagcgg tcaaagaagc tgcgcattca tagaccttaa
    21001 gacgcaataa ccaactttag acaggagaaa tcctttaaag gagccgaaat atggcaaacg
    21061 cgaaatcaac tgaaaaaacc acggcggtcg tcggtacgag tgttatccgt aatgatgtgt
    21121 tggaaaaagt gaccggcgcg gccacttacg tcgacgacat ccagtttggt aataaattgc
    21181 tatacgcccg cgctgtacgc agcccacatc cgcacgcgct gatcaagcat attgatacca
    21241 gcaaagcgga aaaactccct ggtgtcaaag ccgttgtgac tggtaaagac ttccctgaac
    21301 gcatcggtct ttaccttaaa gataagacca tttttgccac cgaccgtgtg cgcttcttgg
    21361 gagaaccggt agcggctgtg gctgccgtta gcgaagaaat cgccgaaaaa gcctgcagcc
    21421 tggtggaagt aaaatacgaa ccgctcgagg gtgtttttga cgctgaatat ggcgcttcag
    21481 aaaaagcccc cgtcattcac cctgaccttg gcaaatatga ttgtgtcaat ttcatcttcc
    21541 ccaagcccgg gaccaacatt tccaaccact ttaaaattcg caagggcgat gtggaagccg
    21601 cctggaagca gtgcgcgcac atagtggaaa agaccttcaa agtcccacat attcaacacg
    21661 tgcctattga aacgcatggc gcggtagcca tgatggatga aaaagggaaa gtcactgtgt
    21721 gggcggcgtc acaatcaccg ttcgcccaac gcaatctcat cgccaaagcc ctgcacattt
    21781 cacacagcga cctgcgcgtt atcgcgccgc ttgtaggcgg agggtttggc tccaaagccg
    21841 gtctcaccat ggaggccatc cccgtagccc ttgccaccaa ggtcaaaggc taccccgtga
    21901 aatttatact cacccgcgaa gaggaattct atactaactt cgtgcgccag ggtctcacca
    21961 tccacgtgaa agtcggctgc gataaagatg gcaatatcct cggtctcaag aatgtgatgt
    22021 actgggatgg cggcgcctcg actgagtacg gcacgaacat tacccgcgct ggtggttatt
    22081 ccagttccgg tccttatgat atccccaatg tgtggacaga cagtatctgc gtttacacca
    22141 accaccccat cggcggcgcc taccgtggtt ttggtatgtc tgagctgcac accgggatcg
    22201 accaggcgat tgacgaactg gcagaagtag ccggttttga ccgtgtcgag ttcctcaaaa
    22261 agaacgccat ctcagaaggc gacattgtgg tcactggtat gaccatgcat gccactggca
    22321 tccagcaatg catcgataat gtagccaaag ccatcgaatt ttcaaagaaa gaagaacctt
    22381 cttctcctga ccgtttgcgt ggtaagggca tcgccatcat gtggaaagcc cccgccatgc
    22441 cccccaacgc cggctcgagc gcctgggtgg aactctctga agacggtaaa gcgaccgtcg
    22501 gacttggcgg gcaagaaatt gggcagggta ctttcaccgt gatggcccag attgctgctg
    22561 aaaccctggg cgtcaaatat gaagacgtgc gcatcgccgg accggtagac acgcaataca
    22621 gcccttacga atggcaaacc gtcgccagcc gcctcacctg gagcatgggg aacgcagtac
    22681 gcctggcggc gctcgacgcc cgtaaacaga ttttggacgt ggtggctgaa gcctggaatg
    22741 aaacaccaga agacctggat atcgtcaacg gcatggtcgt ctcttacaag agcgaacagg
    22801 aaaccccgtt ggccaacctg gtcatctatg gtcttcccaa gcccaatgat gaaggctgga
    22861 tgggcggccc gattgttggt cgcggttcct tcatgcccaa gtacgtcacc aacctggacg
    22921 cggagaccgg tcagggcgcg cgcgctgtgg tacattacac cgtgggctgt gaagggctcg
    22981 acatcgaaat tgaaaagaac accggccgca tccacgtgat caaagctgcc gcttcctttg
    23041 acgtgggcaa ggccatcaac ccggcgctgg tcaaagcaca ggttgaaggc ggctttgtgc
    23101 aggggctcag ctccgccatg ttcgaagaaa tccgcctgcg caacggcatc atgaccaacc
    23161 ccagctttgt ggattaccgt atcgccacgg cggccgacgc cgacttcaaa ctggatactc
    23221 agtatgtcga ggtgccgcag gatgacggac cctggggcgc gcgcggcgtg ggagagcacc
    23281 ccatggtgcc caccatcgca gcactgggta acgccatcta tgacgccact ggcgtccgcc
    23341 tgcaaggccc gccgtactca gccgaaagca tctacctggg tatgctcaac gccggcgttg
    23401 tcaagtagaa taacctttga atgccggatg ttctcatccg gcatttcact gaaaaccaca
    23461 aggagaatta tgactgacca ttacgacctg atcatccgta acgcgacact gcgcaacgag
    23521 ccaaaaagca cccaagatat cgccattcaa gatgggaagg ttgcgctcat cgccaacaag
    23581 atagaagcca aagccgccag cgaactggat gcccgcggta acctggtgac agaatcctat
    23641 gtcaataccc atctgcacat gtgcaaggtg tatacgctga tgatgatgga cgaagccgcc
    23701 ttgaaagact atcacggcgc cgacatgggc aaagccatga ccgccatcga actggctgcg
    23761 cgtgtgaaag aaaaatatga cgaaaaatgg attatcaaga acgtcagaaa ggccgctgcc
    23821 ctggctgccc gttacggctg cacccacatc cgcgctttcg ccgatgtaga ttcaaagggt
    23881 aagctcgaag gaataaaagc gcttatcaaa gcgcgcgacg aattcaaggg catcgtggat
    23941 attcagactg tcgcttttgc ccaggatgga ttggttcgcg agcccggtgc tgacaaactg
    24001 atgcgccagg caatggatct tggcgctgat gtggttggcg gcattccctg gatcgaatac
    24061 accgatgctg atatcgccga gcatgtgcgc atctgctatc aactggcaaa agagtatgat
    24121 aaacccgtct ccatgctggt ggatgatgct ggtgataacg ggttgcgctc gcttgaggtg
    24181 atggcgctgg aaaccatccg tactggctgg cagggacgcg cgctggcaca ccacgcccgc
    24241 gcgatggcgc tctaccccaa gccgtacctg caaaaactcg ccgcaatcct caagaaagcg
    24301 gatatgggcg tggtgagcga tccgcatacc gggccgctac acgcgcgtgt acgtgaactg
    24361 ctggaagaag gtgtgctcgt ttgccttggg caagatgata tctccgacgc ctactaccct
    24421 tatgggcgca ataatatgct tgaggtcgcc ttcctcaatt ctcacttgct ctggttcacc
    24481 acccgcgaag acatggaagt gctctatgat atgatcacca ccaaaccggc tcgcgcgatt
    24541 ggtctcaaag atttcgagtt aaagaagggt gcccccgcca acctggtggt ccttgaccag
    24601 cccaacgtgc ttgaagcgct gcgctaccat gaaaaaccgg tgtatgtgat cagccatggc
    24661 aagcttattg accaggcgaa gatggacaag atcattcagg aaaattcgta gcccgctatc
    24721 agttgctatg aagcacgcga ttatgctatc ctaataaaag tgagaaagga ggaagagccc
    24781 tggttttacc aatacgaata gttgaaaacg caagtgtatt taaaactagc aaagaaggag
    24841 aaaaaaatgt tta
//
LOCUS       DAOTLW010000031        24732 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_2351_length_24732_cov_9.307574, whole genome shotgun sequence.
ACCESSION   DAOTLW010000031 DAOTLW010000000
VERSION     DAOTLW010000031.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 24732)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 24732)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24732
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2351_length_24732_cov_9.307574"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(155..1183)
                     /locus_tag="P5516_06125"
     CDS             complement(155..1183)
                     /locus_tag="P5516_06125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92051.1"
                     /translation="MNWLKKGAQMNRSEKLRQKRQPAAQTRSAVPAAAASRLAADRVV
                     ARRASTSAENRHSVGTQPRRKVVYSVGANGVETRLPALPILRFSWQWVSGILTILLLM
                     FIIVLVNTPIFKVNSFEVHGLSTYSSADFNPLLNGRRDSIFLVNTNEISDSILVAFPE
                     LVGSQVEIGLPNRIVITAAERKPVVLWHSSTGDYWIDAEGVILAPRGEGSGLLTIESA
                     SLPPLLVERHDPRNFTEYVTMVFERKERPSNPSERAAHIRPEVLEAALQMNSLLPQGA
                     TLLYDSVSGMGWRDPGGWEVYFGNDLSNIQMKLVMYQVILGRLQELGISPEMISVEHV
                     DAPYYRME"
     gene            complement(1180..2286)
                     /locus_tag="P5516_06130"
     CDS             complement(1180..2286)
                     /locus_tag="P5516_06130"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HRT92052.1"
                     /translation="MTGKIRLAVIFGGRSGEHEVSLMSARSLLAVLDADRYAITQIGV
                     TPAGKWLHGDDVIGRFEKHDYTTLSPVALLPEPLNGALFAVKNGQMERLPDIDVVFPL
                     IHGTFGEDGTLQGLLELADLAYVGGGVLASAVGMDKSLCKDVLRAHQIPVVDSLLLNR
                     SSIRTDINASIEQIEADFAYPVFVKPVNLGSSVGITRCSNRSDLMKGLLEAARFDRRI
                     LVERAINARELEVSVLGNENPIASVPGEIQPQEDFYTYSAKYLTNQSRLIIPADLDED
                     TSRLVSDTAVSAFRAIDGAGLARVDFLLDRKTGEVFVSEINTIPGFTAISMYPKLWQA
                     SGISYTELINRLVELALERKHDRDITVRTYGRDG"
     gene            complement(2283..3203)
                     /gene="murB"
                     /locus_tag="P5516_06135"
     CDS             complement(2283..3203)
                     /gene="murB"
                     /locus_tag="P5516_06135"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HRT92053.1"
                     /translation="MDEKVLLKRFAQRLKKDVPLANLTTAKVGGAARYFLNAVNAAQL
                     AEDVAFLWAEEIPFFILGSGSNILVSDAGVDCVVIHNKANHIHLVEKGDSSYVQAESG
                     AILITAARQAALRGYTGLEWATTIPGTVGGAVYGNAGAYGASMQQHLLLVDILHRGKG
                     CLSLNTEQMGYSYRSSALKREPGMAVILSARLAIKKGDKERIKELINEFSARRKQTQP
                     PGPCIGSTFKNPAGDSAGRIIDSLGLKGTRVGGVQVSPVHANFFVNEGSASARDYYDL
                     IHLVQRIVEEKTGIKLEPEFELIGDWKEQA"
     gene            complement(3225..3416)
                     /locus_tag="P5516_06140"
     CDS             complement(3225..3416)
                     /locus_tag="P5516_06140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92054.1"
                     /translation="MNPPRKNSYENVNHDFDTPRYFSEFPRIQVKRQINQPVADFRAL
                     SLQDRVELMEKLIERVREL"
     gene            complement(3416..4795)
                     /gene="murC"
                     /locus_tag="P5516_06145"
     CDS             complement(3416..4795)
                     /gene="murC"
                     /locus_tag="P5516_06145"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HRT92055.1"
                     /translation="MMKRIHLIGIGGSGMSSIARVLLEQGLTVSGSDRTLSPLALNLR
                     AAGAIVYEGHDPQHIEGVDMVVRSSAIADDNPEVAAALALGIPVLKRQDFMRDLTRER
                     LCIAIAGSHGKTTTSTMMAWSLVQLGLDPGYILGGVSRNLANNAHAGSGELFVVEADE
                     YDYMFLGLNPAVILLTNVEYDHPDCFPTAEKYQAAFSQFVHQLEPGGLLVTNADDPGA
                     AAKRHDIPESARVVTYGLNGPADYSAIDWRVNAAGGYDFKISALQIHAPVAQVSLAVP
                     GMHNVRNALGVLSALHALGFPVQQAADALSFYSGSSRRFEVIGEVAGITIIDDYAHHP
                     SKISATLSAARVRYPRRRLVVVWQPHTYSRTQALESDFIRAFDDADLLVVTAIYAARE
                     QARAYSAEQMVEKMKAPSVSYIPQIPDVVDFLLKTLQKGDVLIVLSAGDADQVCREVR
                     TRLQERKGE"
     gene            complement(4792..5334)
                     /locus_tag="P5516_06150"
     CDS             complement(4792..5334)
                     /locus_tag="P5516_06150"
                     /inference="COORDINATES: protein motif:HMM:NF014704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009403 - toxin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HRT92056.1"
                     /translation="MISLDVLLWVFIVLFALVGAMRGWAKELLVTFSIILAMFSLTVL
                     EAFLPFFKETIQQSQPSTVFWLKSGILMALVFFGYQTPKIPRLAESERFIRHVLQDTM
                     LGAVLGGINGYLIFGTVWYYLHSTGYPYPFVLPPDALTQSGQTALRWIENLPPAWLMT
                     TPTIYIAVAVCFVFVLVVFI"
     gene            complement(5336..6442)
                     /locus_tag="P5516_06155"
     CDS             complement(5336..6442)
                     /locus_tag="P5516_06155"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HRT92057.1"
                     /translation="MRLLICAGGTGGGVYPALAVLQTVADRVEQTLWVGGQGGMEAAL
                     VARTGVPFAEISAAGVHGVGLAALPGNLIKLGRGYSQARRILRDFQPHVLFFTGGYVA
                     VPMALAGAGVRKLLYVPDIEPGLALKALAKFANIIALTDESSTRYFSKKKQVVVTGYP
                     LRGELQKVPREQALQTFKFTAELPVLLVIGGSKGAHSINQAVFDNLPTLLHKTQVLHL
                     TGQQDWEEANSIRLALEVHLGARYQPYPYLHEEMSAAFSCANLVLSRAGASTLGELPF
                     YGLPAILVPYPYAWRYQKVNANHLTQSGAALTVENNELSDRLGALVDELLSDPQRLTD
                     MGKKMGVMAAPDAARKIADLLVLLAEEQGKERRA"
     gene            complement(6366..7610)
                     /locus_tag="P5516_06160"
     CDS             complement(6366..7610)
                     /locus_tag="P5516_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HRT92058.1"
                     /translation="MTTTGLNPRPRRTHFRRLSLRESFDLPLAVVVLTLFAVGLLMIY
                     SASWQYASVQGFSEYQMVMRQMLIGLAGLGGIVIITLIDYHQYQRFVVPGMLLIVSLC
                     LLVLLFGSETQFGARRGLFSGSIQPSEFAKLGVIIYLSFWLYNKRGRLHMWSYGLIPL
                     LAILGVTAGLILMQPDISAAATIVLLGGVLFFIAGGKMSQILVVVVATALLGFLLVKV
                     SSTASTRLSDYWNGLQDPKNASDHVKWSLEAIINGGLFGVGIGRSTTKFIGLPVATTD
                     SIFAVITEETGLLGAFAVIILYVLLLWRGLTIADRAPDTLGKYLASGITLWIFIEAII
                     NIGVLVNLLPFAGNALPFISSGGSSLITTLAAVGILMGISRQSKLAKLSAERSPRNAV
                     VDLRRRNRRGSVSRAGSAADGR"
     gene            complement(7607..8986)
                     /gene="murD"
                     /locus_tag="P5516_06165"
     CDS             complement(7607..8986)
                     /gene="murD"
                     /locus_tag="P5516_06165"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HRT92059.1"
                     /translation="MNRFNGKRVLIIGAARQGLALARYLINQQALVTLNDQQTPEQLQ
                     QVMQPFAGSGVEWALGGHPLHLLDGKDLVCVSGGVPLTLPLVQAALERGIPLTNDSQI
                     FMETVKAPVVGITGSAGKTTTTSIFGRMAAAALKSPCKAWVGGNIGNPLIEFSEEIDA
                     DDLVIQEFSSFQLELMTTSPHIAAVLNITPNHLDRHADMDEYTRAKSRILFHQSTADI
                     AVLNREDMGAWNLRPLVRGALLSFGMERSPSVGNQVYLKDPNIHAEVEGKATELFPVS
                     LISLRGKHNLMNVLAACALALACDFPVEAMVRGIEGFNGVKHRLQFVREVNGVRWYND
                     SIATAPERSLAALQSFQEPIILLLGGRDKNLPWQPLAQEVKKRARHAVLFGEAGSKIA
                     AALGTPTEVDYLQSISQVGTLQEAVLQAARLASQGDVVLLSPGCTSYDAFKDFEERGE
                     AFIEWVNHL"
     gene            complement(8994..9986)
                     /gene="mraY"
                     /locus_tag="P5516_06170"
     CDS             complement(8994..9986)
                     /gene="mraY"
                     /locus_tag="P5516_06170"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HRT92060.1"
                     /translation="MKETALALSLSGLGFILSVIWGGPLLRVLKHFKMGKIIRVEGPE
                     SHITKMGTPTMGGLVIVGTVVLFTVLLNAASILGLHLSGRSVLLPVVVMVSFSLLGAI
                     DDWEGIRGPRRGLGMRARTKFLLQVILALIAAFILKNVLDVPDMYWPGLKINIELGVW
                     YIPLATFIIVAMSNAVNFTDGLDGLAGLICATAFAAYGGIALMQGNIFVARFCFTVVG
                     ALFGFLWFNVHPAMMIMGDTGSLSIGAVLGVVALMTGQWVLLPIIAIIPVSEAASDVL
                     QILYFKATKGKRLFKMAPLHHHFELLGWSETQIVQRFWLIGLLAAMIGMALALG"
     gene            complement(9983..11401)
                     /gene="murF"
                     /locus_tag="P5516_06175"
     CDS             complement(9983..11401)
                     /gene="murF"
                     /locus_tag="P5516_06175"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HRT92061.1"
                     /translation="MLTLADVIEALTGVRPIGATPGISEGVVDSRLAIPASLFVAIKG
                     NRTDGHQYVSHAFANGARFALVQHEIDPSIPVVDLGKPISADHAFPPLPFALCVKDTT
                     KALQTVAAFWRRKFTTRVIGITGSVGKSTTKELTAEILSQRYRTLKNQGNFNNEVGLP
                     LTLLRLGPGYDRVVLEMGFYEPGEIASLCEIAKPQVGILTNIGTVHASRAGSQETIAH
                     GKSELVRALPPAPTGSAILNIDDPFIREMANLTQAKVIYYGLDSKADLWADEIESRGL
                     DGIHFKIHHGADAFNLQAPLIGRHSIHTILAAAAAALNEGLTWQEIILGLQHATSQLR
                     LVAVKTRQGALILDDTYNASPESMLAALNLLNELPGRKIAVLGGMNELGQYEQLGHNK
                     VGIRAAEVVDKLIAFQEKAQLIATAAQKAGLPGEFIEIIDDENEVIQALNLTLRPGDV
                     VLVKGAHSLRMDRIVQAMEVEG"
     gene            complement(11410..13182)
                     /locus_tag="P5516_06180"
     CDS             complement(11410..13182)
                     /locus_tag="P5516_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013100.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HRT92062.1"
                     /translation="MRKRFMRYHFLGISLSVFAVVIAVQMVRIQSSTHADYLNDWAKD
                     YGYELRTIESERGYIYDRYGHLLAGNKEVYEVGIELQFVRNPSAIASTLANVLGLDYQ
                     EVFNAANTKYVPGQQVYVTLADFVESYKIKEINDLKTQFENNNPYGLDTALPSLRGLT
                     WTAHLRRIYPEGNLAANILGFFTYYDREGGAGYFGVEEKYNTLLAGIKRNVVIPLDPY
                     ELQEIPTAPAGASLVLTIDREMQRTVEGILDKAVKTNGAAGGTIIVMDPNNGEILAMA
                     SNPRLDPNAYWENTKVFPPGVPYNKAVNQVYEPGSVFKILTMAAALDAGVVTSDTPFT
                     DTGQIEVGGWMIYNWDRGAWGNQNMIGCMQHSLNVCLSWIATQLGPTRFYDYLERFGI
                     GHRTNIDLAGEEVYPLSVPGDPMWYTVNLATNSFGQGVAVTPVQLLMAASAIANEGQM
                     VAPHVLKSYIQDGQQFDTAPQVVGRPITAETAKTLTDMLAISLEEEASTALVDGYRVA
                     GKTGTAEIAVNGQYSSNKTNASFVGWGPVDDPQFIVFVWLEKPSTSIWGSIVAAPVFS
                     NVVQEIVVLMNLPPDEIRMSLKEE"
     gene            complement(13182..13649)
                     /locus_tag="P5516_06185"
     CDS             complement(13182..13649)
                     /locus_tag="P5516_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92063.1"
                     /translation="MRLIQALKQLPWKIHARTVGFFLLSLVVVLVVASVYLSISGLAA
                     NAGLDAYRLDLKRQDLEREIAHRKATLALITAAPAMEERALSMGFKKIAPEDALYVIV
                     PGYSGRRTMVAAPPPGINETSRQLIKSVYRESLWDWLFTGVNSLGTGFLGDGS"
     gene            complement(13715..14659)
                     /gene="rsmH"
                     /locus_tag="P5516_06190"
     CDS             complement(13715..14659)
                     /gene="rsmH"
                     /locus_tag="P5516_06190"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HRT92064.1"
                     /translation="MKNAHDSAAKPPHVSVLYHQIITALRPVSPGLYVDATIGAAGHA
                     WGILEKSSPDGQLLGLDTDPQALALSHQRLSIFHQRFHLLHSSYTELRNALLTLGWDH
                     VDGIVADLGVSSMQIDSPERGFSFLNDGPLDMRFDPSQPVSAETLVNHSTESELADLI
                     WRYGEERRSRHIARVIVQNRPFTSTLQLAETIKKASYGIRSEIHPATRTFQALRIAVN
                     HELDSLQIFLPQAVESLAPGGRLAVISFHSLEDRIVKQFFRRESRDCICPADQPVCIC
                     GHKASLIEVTRHPITPDGIEVSSNVRARSAKLRVAEKR"
     gene            complement(14659..15102)
                     /locus_tag="P5516_06195"
     CDS             complement(14659..15102)
                     /locus_tag="P5516_06195"
                     /inference="COORDINATES: protein motif:HMM:NF014439.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HRT92065.1"
                     /translation="MFKGFTGCYLHNIDEKGRLTFPVSFRDLLGENPFILKGFDQNLL
                     VMNANRFELLYERINNMNMANAQTRQLRRYIFSTAAQIEFDKAGRFIIPQPLRRIAGL
                     DGTALIVGIGKDIEIWNPDTYKIQEDQGEGPSSAAELVSSFDLTI"
     gene            15293..16240
                     /locus_tag="P5516_06200"
     CDS             15293..16240
                     /locus_tag="P5516_06200"
                     /inference="COORDINATES: protein motif:HMM:NF022114.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HRT92066.1"
                     /translation="MRSFFARFSILVVVLLGFTMLNGCITINLGGATPDSGQTLEPAP
                     TTPPPAAVQTPRQVLNEERLLNADYLSPLFQVPITLVGGVFSGVVDGSELKVTLQPGI
                     QYGDLNDDGVEDAAFILAESTGGTGVFSSLVVVFSEAGRFVQAPGVMIDDRPVIESLT
                     IENGVVKLQALVHSFNDTMVNPSTRIRAEYSLLDGKLVQTRLSSAFSGSEEHVITIDS
                     PRDGDTISGSVRLIGSMPIGPFENTLLLRITDLSGGELLHEGFMVQTEDLGAPATFDN
                     TVLIPGIAPGTRILLTLSELSMADGSPIALDSVLLVVGE"
     gene            complement(16352..16891)
                     /locus_tag="P5516_06205"
     CDS             complement(16352..16891)
                     /locus_tag="P5516_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92067.1"
                     /translation="MRFLIFSISIMLLLVSCNPTLSDTPDFISPLSTATARPVINDEN
                     PSPPAPTIAKPDQPVDSLTPLKTPNSFEPGPGDELKQRSAAFVDNAQLMILESDPIQV
                     NLQIKGSLPTPCHILRVAISPPDKENHIAISVYSLVDPAVVCIQVLQEFETSVPLGAY
                     PYGHYLVYVNDMLVGEFDG"
     gene            complement(16985..17368)
                     /locus_tag="P5516_06210"
     CDS             complement(16985..17368)
                     /locus_tag="P5516_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HRT92068.1"
                     /translation="MRKVKLAVNGTLMRGLELNHNLLSVGAVFLCQTVTAPTYRLWSI
                     GDLYPAMLRDDHLGAAIHVEIWSLSASALVAVLQQEPPGLCLGRVELQGGELVFGVLA
                     EPYLISGQQEITRWGGWREYRQSQP"
     gene            complement(17379..18902)
                     /locus_tag="P5516_06215"
     CDS             complement(17379..18902)
                     /locus_tag="P5516_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HRT92069.1"
                     /translation="MLAKVNACAVIGLDGVVVDVEVDLTPGIKPPIIIVGLPDVSVQE
                     SRERVQSAIRNTGLEYPRKRVRVNLAPATVRKEGSAYDLPIALGILIANRQLPPQCLQ
                     DALVMGELSLDGSVRHIRGVLPMAAVARAQGYKRVYVPAEDAPEAAIFPDLEVIPVSS
                     LADLFQHLVGQVIIDPQPALAVEQVSALVQTDFREIKGQEHVKRALEVAAAGAHNLLM
                     IGPPGAGKTLMARALPSILPSMTLEEALDVTRIYSVADALPEGIPLLKSRPFRAPHHT
                     ISHAGLVGGGNWPHPGEISLAHRGVLFLDEFPEFGSRTLEVLRQPLEDKIITISRAQG
                     TLTFPANFQLVAAMNPCPCGYYGDPTRACTCAPGIVTKYQRRISGPLMDRIDIHVEVP
                     RVDYEKLSDARLGEPSAVIRTRVESARQIQRERFADTSLTSNADMHPAQVRQYCSLDE
                     SCRGLMRTAMNQLQLSARAYHRILKLARTIADLAGSPHISTIHLAEALQYRPRVSDV"
     gene            complement(19233..20162)
                     /locus_tag="P5516_06220"
     CDS             complement(19233..20162)
                     /locus_tag="P5516_06220"
                     /inference="COORDINATES: protein motif:HMM:NF018024.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1028 domain-containing protein"
                     /protein_id="HRT92070.1"
                     /translation="MPERRFPFAHTFSIVARDPKNGELGVAVQSHWFSTGSIVAWAEP
                     GVGAIATQSMAEVSYGPLGLSAMREGKPASVVLANLLAKDANREVRQVAMVDGKGTVA
                     VHTGQRCIAEAGHQTGYQYSVQANMMLRNTVWPAMAAAYEAAKGSLADRLLAALDAAQ
                     AQGGDIRGQQSAALLVVSGESHNEPWKGVLVDLRVEDHSEPLVELRRLLTVQHAYEHM
                     NRGDELLSQGLIDQAFSAYEQAAALAPHLHELPFWQAVTLLDSGREQEALPILKRVFQ
                     ANPNWLLLLERLPAAGLLKDDRRMLRRVRKLLG"
     gene            complement(20191..21099)
                     /locus_tag="P5516_06225"
     CDS             complement(20191..21099)
                     /locus_tag="P5516_06225"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT92071.1"
                     /translation="MTIPDLSLLAATSWQDYELLDSGAGMKLERFGAYRLIRPEAEAV
                     WQRALPENEWRAADALYKPAPEENGGHWEFRKKMPDQWKLSYGQLAFWVKTSSSRHLG
                     VFPEQSCQWDWAEKVIHLSGGKPRVLVLFGYTGIASLSAAAAGAQVTHLDASRKVITW
                     ARENQTLSRLDSLPIRWILDDALKFVQREQRRGSCYDALILDPPKFGRGPKGEIWDFY
                     KLLPDLLAACRAILSPDPLFVQLTAYAVKASAATMYNAILEMTARFKGITQAGEIGLL
                     ERSAKRFLSTAIFARWCRADIHQEMG"
     gene            21207..22343
                     /locus_tag="P5516_06230"
     CDS             21207..22343
                     /locus_tag="P5516_06230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HRT92072.1"
                     /translation="MFQAPERRNTDSVKWGLFTEDVLPLWVADMDFTSPPVVIEALQQ
                     RVSHGVFGYALESQELKDLIVQRMSRLHQWRISPEDILLVPGVVSGFNLVCQAVVHPG
                     ESILLQPPVYPPFFEAPVKAQARTVYNNLQPGADGQYRVDLDAFAKSIEKDTKAFLLC
                     NPHNPLGRVFTREELLGMAGECLRHEMIIIADEIHCDLVYKGYQHIPIASLDDEIAAS
                     TVTLIAPSKTFNIAGLDCSILICQNEELRKRILHSKRGLMGGVNLLGMTAAAAAYREG
                     ASWLSEVLAFLESNREHLMAYIEHNLPRIHMVKPEATYLAWLDCRELELAPSPREYFF
                     QRARVALNDGRDFGEPGTGFVRLNFGCSRETLDNALERMCHSLS"
     gene            complement(22397..22609)
                     /locus_tag="P5516_06235"
     CDS             complement(22397..22609)
                     /locus_tag="P5516_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HRT92073.1"
                     /translation="MAERFVGTVKWFNPAKGYGFIGRENAEDVFVHFSAIEMEGYKVL
                     KEGQQVEFSIEKGPKGLQAAEVKPVE"
     gene            22760..24172
                     /locus_tag="P5516_06240"
     CDS             22760..24172
                     /locus_tag="P5516_06240"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT92074.1"
                     /translation="MRNERKLWLLFFLLLPLGFMAFPKAGAAPENLTLSLQNDRAALV
                     NGDAQMELTAIQQALEFQTWRGDLWQRAGRLYMDQGDYQNALHSFEKAQELGQLEPLG
                     QVWLSDMLIANGYSDVAKYVLQSIHTDDVFILLQAAALMAPLNDHEGLRQLLQKAYAS
                     EPTNSEVNYQMGIYLMTEDPGRAVAYFELAKRDQSRKAAAAYLVENIQQYGALEDHGE
                     WYLYAGQALARINEWNAAFQAFSKAVQSLPDNAIALALLGESQFQLGLDGRQNLEKAW
                     ELDPEGEITNGVLGIYYQRQGNLEKALEHLRIAQKSNPSAAIWILESGRTLAAMGDLE
                     KALTEFSLAVTVDEKNADLWRVLAEFCVLHRYQLEDVGLPAARQALTLEPDNPVLMDL
                     LGTVYLQLADLDSAERFFLRALERDPEQAAILIHLGQLNLIREEKEIAFGYLRRAEAS
                     ARDWRLRDLANRLLRENGAQ"
     gene            24212..>24732
                     /locus_tag="P5516_06245"
     CDS             24212..>24732
                     /locus_tag="P5516_06245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92075.1"
                     /translation="MNKPTHLFFRFLLFCCALTLTGCEKIGIHFPQSGAVPPGGLLFE
                     DDFSHPPNGWGSMETRGGEIEFIFDGMMISIFLPNFMYWSVNGKEFSDVKVKVDAVLA
                     DGPQDDTFGVICRFQDDRNFYGFALSHDGYYGVFKYVDGKMNVLSNEGNLGFSEAIRT
                     GGVVNHLEATCER"
BASE COUNT         5809 a         6452 c         6916 g         5555 t
ORIGIN      
        1 atatccacca cgttgccttt tttaatgccc agggccggct cgatgcccac acccagaatg
       61 cgcatatctg tgtcgccttc caggcgggcg accagggtgc agatcttggt ggtgccgata
      121 tcaatcccga cgacaatagg ttcttccata gtgcttactc catccggtaa tatggcgcgt
      181 caacgtgttc cacgctgatc atctcgggag aaatacccag ctcctgaagg cgcccaagaa
      241 tcacctgata catcaccagt ttcatctgga tattgctcag gtcgttgccg aaataaactt
      301 cccaaccacc gggatccctc cagcccatac ctgaaactga atcataaaga agcgtcgctc
      361 cttgaggaag gagagaattc atttgcagcg ccgcttcaag tacttccggg cgaatatggg
      421 cagcgcgctc ggaagggttc gatggtcttt ccttgcgttc aaaaaccatg gtgacgtact
      481 cggtgaagtt tcgcgggtca tgccgctcaa cgagcagcgg aggtaaggac gcgctctcaa
      541 tcgtcagcaa accgctgccc tctccacgcg gagcgaggat gacaccctcg gcgtcaatcc
      601 agtaatctcc ggtgctggaa tgccataaaa cgaccggttt tcgttcggcg gcggtgatga
      661 caatgcggtt gggcaatccg atctcaacct ggctgccgac cagttcagga aaagcgacca
      721 gaatggaatc agatatctca ttggtgttga cgaggaagat cgaatcacgc ctgccgttga
      781 gcagcggatt gaaatcagcg ctggagtagg tactcaaacc atgtacctca aagctattga
      841 ccttgaagat gggtgtgttg actaagacga tgatgaacat caataacagg atggtgagga
      901 tgccagacac ccattgccag ctgaatcgca gaatgggcag cgcgggcagg cgggtttcga
      961 cgccattggc gccgacgctg tacaccacct tgcggcgggg ctgcgtcccg actgaatggc
     1021 ggttttccgc tgaggtgctg gcgcggcgag cgacaacccg atctgctgcc agccgggaag
     1081 cggctgcagc cggcaccgca gaacgcgttt gcgctgccgg ctggcgcttc tgccgcagct
     1141 tttcagaacg gttcatctgg gcgcctttct ttaaccagtt catccatctc tcccgtaggt
     1201 acgcacggtg atatcgcggt cgtgttttct ctcaagcgcc aattcgacca ggcgattgat
     1261 aagttcggta tatgaaatgc cacttgcctg ccaaagtttt ggatacatgc tgatggcggt
     1321 gaaaccagga atggtattga tttcgctgac gaaaacctct ccagtcttac ggtcgagcaa
     1381 gaagtccacg cgcgccagac cagcgccatc aatcgcgcga aaggcgctga ccgcggtgtc
     1441 gctgaccagc ctactggtgt cctcgtccag gtcagccggg attatcaggc gggactgatt
     1501 ggtgaggtat ttggctgaat atgtataaaa atcttcctgc ggttggattt ctccgggaac
     1561 ggaggcgatg gggttctcgt tgcccagcac gctcacttcg agttcacggg cgttaatggc
     1621 acgctccacc agaatccggc ggtcaaaacg agccgcctcc aataatcctt tcattaaatc
     1681 agagcgattg gagcagcggg taattcccac cgaggagccc aaattgaccg gtttcacaaa
     1741 gacagggtaa gcaaagtcgg cttcgatttg ttctatgctg gcgttgatat ccgtgcgaat
     1801 ggaagagcgg ttgaggagca acgaatcgac cacgggaatt tgatgagcgc gcagcacatc
     1861 tttgcaaagg cttttatcca taccgaccgc agacgccagc acgccgccgc caacataagc
     1921 caggtcagcc agttccagta agccttgcag cgtgccgtct tcaccgaaag taccatgaat
     1981 gagcgggaag actacatcga tatcaggcag gcgctccatc tgcccgtttt ttaccgcgaa
     2041 caacgcgcca ttgagcggct caggcagaag ggcgaccggt gagagcgtgg tgtaatcgtg
     2101 tttttcgaag cggccaatca catcatcacc atgtagccat tttccagcag gagtgacgcc
     2161 aatttgggta attgcgtaac ggtcagcatc aaggacggca agaagcgagc gggcgctcat
     2221 cagggagact tcatgctcac ctgatctacc gccgaagatc actgccaagc gaattttccc
     2281 agtcatgcct gttccttcca gtcaccaatc agttcaaatt cgggttccag tttgataccg
     2341 gttttttctt ccacgattcg ctgcacgaga tgaatcagat cgtagtaatc acgggcgctg
     2401 gcgctgcctt cgttgacgaa aaagttcgcg tgcacggggc ttacctgcac gccacccaca
     2461 cgggtgcctt tgaggccgag cgaatcaatg atgcgcccgg cggaatcacc cgcggggttc
     2521 ttgaaagtag agcctatgca tgggccggga ggctgggttt gcttcctcct ggctgaaaat
     2581 tcgtttataa gttcttttat cctttctttg tcgccttttt tgatggcaag ccgggcggag
     2641 aggataacag ccatgcccgg ttcgcgtttg agcgcgctgc tacgatagga ataacccatt
     2701 tgctcagtgt tgagcgacag gcagccctta ccacggtgca agatatctac caggagaaga
     2761 tgttgctgca ttgaagcgcc atacgccccg gcgttgccgt aaaccgctcc gccgaccgtg
     2821 ccggggatag tggttgccca ctccagcccg gtgtacccgc gtaacgccgc ctgacgggca
     2881 gcggtgatga ggatggcgcc ggattcagcc tgtacatacg aagaatcgcc tttttcaacc
     2941 agatgaatgt ggtttgcttt attgtggatg accacgcaat cgaccccggc atcacttacc
     3001 aggatgttgg aaccgcttcc cagaatgaag aaggggattt cttcagccca caggaaggcc
     3061 acatcttccg cgagttgagc tgcatttacc gcgttcagga aatagcgcgc cgcaccaccc
     3121 actttggcgg tggtcaggtt cgccagagga acatccttct tcaggcgttg ggcaaaacgt
     3181 ttgagaagta ccttctcatc catgggcgcg gtcatgctcc tgtcttagag ttctctcact
     3241 ctttcaatca gcttctccat caactccacg cggtcttgca ggctcaaagc gcgaaaatca
     3301 gccacaggtt ggttgatctg gcgtttcacc tgaatacggg ggaactcaga gaagtagcgg
     3361 ggcgtatcaa aatcgtgatt gacattctca taactatttt ttctgggcgg gttcattatt
     3421 cacctttcct ttcctgcaga cgggttctca cttcacgaca aacctggtca gcatcaccgg
     3481 ctgacagaac aatcaacaca tctccctttt gcagcgtctt taataaaaaa tccaccacat
     3541 cgggaatttg agggatataa ctcacagagg gagctttcat tttttcaacc atttgctcgg
     3601 cgctgtacgc acgcgcctgt tcgcgcgctg catagatggc ggtgaccacc agcaggtcag
     3661 catcatcaaa ggcgcgaata aaatcacttt ccagcgcctg cgtgcgagag taggtgtgcg
     3721 gttgccaaac gacaacaagg cgacggcggg ggtagcgtac acgcgcagcg ctgagtgtgg
     3781 cgcttatctt gctggggtga tgcgcatagt catcaataat tgtgatgcct gctacttcac
     3841 cgataacctc gaagcgccgg ctggagccgc tataaaagga gagggcgtcc gcggcctgct
     3901 gcactgggaa accgagggcg tgaagtgcag aaagcacacc gagagcgttg cgaacgttgt
     3961 gcattccggg aacagccaaa ctgacctggg ctacgggggc gtgaatctga agcgcactga
     4021 ttttaaaatc atacccaccg gcggcgttga cgcgccaatc aatggcggaa taatcggcgg
     4081 gcccgttcag cccgtaggtc actacgcggg cagattcggg aatgtcgtga cgtttcgcgg
     4141 ctgcaccagg gtcatcggcg ttggtcacga gcagtccacc cggttcaagc tggtgaacaa
     4201 actgcgaaaa cgccgcctgg tatttttctg ctgtcgggaa gcaatcggga tggtcatatt
     4261 cgacgttggt aaggaggatg accgccgggt tgagaccgag gaacatatag tcgtattcat
     4321 ccgcttcaac gacgaataac tccccgctgc ccgcgtgcgc gttatttgcg aggttcctgg
     4381 agacgccccc gaggatgtac ccgggatcca gcccgagttg taccaggctc caggccatca
     4441 ttgtggaggt ggttgttttt ccgtgagaac cggcaatcgc aatacacagg cgctcccggg
     4501 tcaggtctcg catgaaatcc tggcgcttga gcacagggat acccaatgcg agggctgcgg
     4561 caacttcagg gttatcatca gcgatggcgg aagaacgcac caccatatcc acaccctcga
     4621 tatgttgtgg atcgtgcccc tcatatacga tcgcgccggc agcgcgcagg ttaagcgcaa
     4681 gcggagaaag ggtgcggtca gagccgctca cagtaagacc ctgctccaac aaaacacgcg
     4741 caatggagga catgccgcta ccgccaatac cgatcaggtg tatgcgcttc atcatatgaa
     4801 caccaccagt acgaagacaa agcaaaccgc gaccgcgata taaatggttg gagtggtcat
     4861 cagccaggcg ggcggaaggt tttcgatcca gcgcagggca gtttgtccag attgcgtgag
     4921 cgcgtcgggc ggcagcacaa atgggtaagg ataaccagtg gaatgcaggt agtaccaaac
     4981 tgttccaaaa atcaggtatc catttatacc gccaagaaca gcacccagca tggtatcttg
     5041 aagcacatga cggataaagc gctcgctttc agccaacctg ggaatcttgg gcgtctggta
     5101 gccaaagaag accagcgcca tcaggatgcc gcttttgagc cagaagacgg ttgatggttg
     5161 ggattgttga atggtctctt taaaaaaggg caggaatgct tcaagcacgg taagcgaaaa
     5221 catggccagg atgatgctaa aagttaccag cagctcttta gcccagccgc gcatggcgcc
     5281 aacaagcgcg aaaagaacga taaacaccca gagcagtacg tccagactga tcatatcagg
     5341 cgcgcctttc tttgccctgt tcctctgcga gcaaaaccag gaggtcagca attttccgtg
     5401 ctgcatccgg ggcagccatc acgcccatct ttttgcccat atcggtaagg cgctgtgggt
     5461 cagaaagcaa ttcatccacc agcgcgccca acctgtctga aagctcatta ttttcaacgg
     5521 tcagcgcggc accggactgt gtgaggtggt tggcgttcac cttttgatag cgccaggcgt
     5581 aagggtaggg taccaggatg gcgggaagcc cataaaaagg cagctcaccg agtgtggagg
     5641 ctccagcccg tgaaagcaca aggttggcgc aagaaaaggc agcgctcatt tcttcgtgta
     5701 gatatgggta aggttgataa cgcgctccaa gatgcacttc aagcgcgagc cttatggagt
     5761 ttgcttcttc ccaatcctgc tgacccgtga gatgcagcac ctgggttttg tgtaacaagg
     5821 tggggaggtt atcaaacaca gcctggttga tggaatgggc gcctttgctt ccgccgatga
     5881 ccagcagcac tggtagttct gcggtgaatt taaatgtttg caacgcctgc tctcgcggta
     5941 ccttttgcag ctctccacgt aatgggtagc ctgtaacgac tacctgtttt ttcttgctga
     6001 aatacctggt agaactttca tcggtcaggg cgatgatatt ggcaaacttg gccaaggctt
     6061 taagcgccaa accgggttca atatcaggaa catagagcag tttacgcaca ccagctccag
     6121 ccagcgccat aggcaccgcc acgtaaccac ccgtgaagaa caacacgtgc ggttgaaaat
     6181 cgcgcagaat gcgcctggct tgagaataac cgcgcccgag tttgatcagg ttgcctggca
     6241 aggcagccaa cccaacgccg tgaacaccag cggcgctgat ttcagcaaac ggaacaccgg
     6301 tgcgcgcgac tagagcggct tccataccgc cctgaccgcc cacccacagc gtctgctcaa
     6361 cacgatcagc gaccgtctgc agcactgcca gcgcgggata cactcccccg ccggttccgc
     6421 cggcgcaaat caacaaccgc attcctcggg ctcctttctg cgcttaattt cgcaagctta
     6481 gattgacgtg aaattcccat taaaataccc actgctgcca aagtagtgat caggctggat
     6541 ccgcctgaac tgataaacgg tagcgcgttg ccggcaaaag gcaataggtt taccaaaaca
     6601 cctatgttga tgatggcttc aataaagatc cacagggtaa tacccgatgc cagatatttt
     6661 ccaagcgtat caggtgcgcg gtcagcgatg gtgagaccac gccacagaag gagcacatat
     6721 aaaataatga ccgcaaaggc tccgagcagc ccggtttctt cggtgatgac cgcgaaaatg
     6781 gaatcagtgg tagcaacggg caggccgatg aacttggtag ttgaccgacc gatacctacc
     6841 ccgaacaagc cgccgttgat aattgcttcg agagaccatt tcacatgatc tgatgcgttc
     6901 ttggggtctt gcagaccatt ccagtaatcc gaaagacggg tggaggcggt tgaacttacc
     6961 tttactaata agaaccccaa taacgctgtg gcgacaacca ctaccagaat ttggctcatc
     7021 ttaccgcctg cgataaagaa aagcacccca cccagcagga caatggtggc ggcggcggaa
     7081 atatctggct gcatcaaaat caagcctgcg gtaacaccta ggatggcaag cagcgggatt
     7141 aacccatagc tccacatgtg gagccgaccc cgtttgttgt acaaccagaa actgaggtag
     7201 ataatgacgc ccaatttggc aaattcagaa ggctgaatgg aaccggagaa gagaccgcgg
     7261 cgggcgccaa actgagtttc agaaccaaaa agcaacacca gaaggcataa gctgacaatc
     7321 agcagcattc cgggcacaac aaacctctgg tactggtggt aatcgatcaa agtgataatg
     7381 acgataccgc ccagcccggc taacccgatg agcatttggc gcatcaccat ttggtattct
     7441 gagaacccct gcacgctggc atattgccaa ctggcggaat agatcatcag cagacccacc
     7501 gcaaaaaggg tgagaacgac cactgccaag ggtaaatcaa aactttcacg cagactcagt
     7561 cggcggaagt gggtgcggcg tggtcgggga ttcaatcctg ttgtcgtcat aagtggttca
     7621 cccattcaat aaaagcctca ccgcgttctt caaaatcctt gaatgcatcg tagcttgtgc
     7681 agcccgggga gagaagcacc acatcgcctt gtgaagccag gcgcgcagct tgtaagacgg
     7741 cttcctgcag tgtacctacc tgactgatgc tctgcaggta atccacttcg gtcggggtcc
     7801 ccagcgcagc ggcgattttt gaaccggctt ctccaaacag caccgcatgg cgtgcccgtt
     7861 tctttacttc ttgggcgagc ggctgccagg gcaggttttt gtctctgccg ccgagaagga
     7921 gaataatggg ttcctggaaa gactgaagcg ccgcgagtga cctttcaggg gcagtggcga
     7981 tagagtcgtt gtaccagcgc acgccgttca cttcgcgaac gaactgcaga cggtgcttta
     8041 ccccgttaaa cccttcaatt cccctgacca ttgcctctac cgggaagtcg caggcgagcg
     8101 cgagagcgca ggcggcgagg acgttcatca ggttatgctt cccgcgtagc gaaatcaggc
     8161 tgaccgggaa tagttcagtg gcttttcctt ccacttcggc gtgaatgttg gggtctttaa
     8221 ggtaaacctg gttacccacg cttggagacc tctccatgcc gaaactcagc agcgcgccgc
     8281 gaaccaatgg acgaaggttc cacgccccca tatcttcacg gttcagtaca gcgatgtcag
     8341 cggtagactg atggaagaga atgcgggatt tggcacgggt gtattcatcc atatccgcat
     8401 gtcggtcaag atggttgggg gtaatattca gcacagcagc gatatgcggg ctggtggtca
     8461 tcaattccag ctggaaactg gagaactcct gaatcaccag gtcatcggca tcgatttctt
     8521 ctgaaaactc aatgagcgga ttcccgatgt tgccgccgac ccaggcttta cacggggatt
     8581 tcagcgcagc ggcagccatg cgcccaaaaa ttgaagtggt ggtggttttc ccagccgaac
     8641 cggtaattcc caccactggg gctttgactg tttccatgaa aatttgcgaa tcattcgtga
     8701 gcgggatgcc gcgctcgagc gccgcctgta ccagcggcag tgttaatgga acaccgcctg
     8761 aaacacaaac caggtctttt ccatcgagca gatgaagagg atgtccgccg agcgcccatt
     8821 caacgccgct gccagcaaac ggttgcatca cttgctggag ctgctcaggg gtttgttggt
     8881 cgttgagggt gacaagcgcc tgctggttga tcaaatatcg ggcaagcgcc agaccctgcc
     8941 tggcggcgcc gatgatcagc acgcgtttcc cattaaagcg gttcatcgtc tccttatccc
     9001 agtgccagcg ccattccgat catggcggcc agcaagccaa tcagccaaaa acgttgcaca
     9061 atttgcgttt cgctccagcc caggagttca aagtggtggt gcagtggagc catcttgaac
     9121 aggcgtttgc ccttggttgc cttgaagtac aaaatctgca gcacatcgct ggcggcttca
     9181 ctcaccggaa tgatggcgat gattggcagc aggacccact gaccggtcat cagggctacc
     9241 actcccaata ccgcgccaat tgacagagag cctgtgtctc ccataatcat catcgccggg
     9301 tgtacattga accaaagaaa accaaaaagc gcgccaacaa cggtgaagca gaagcgggcg
     9361 acaaatatgt tgccctgcat cagcgctata ccaccgtagg cagcgaaggc agtcgcgcaa
     9421 atcaacccgg ccagaccgtc cagaccgtct gtgaagttca ctgcgttaga catggcgacg
     9481 atgataaagg tcgccagggg gatataccat actcccagct cgatattaat ttttaatccc
     9541 ggccagtaca tatcaggtac gtcaaggaca ttcttgagga tgaaagccgc gatgagggcg
     9601 aggatcacct gtagtaaaaa cttggtgcgc gcgcgcatgc ccagcccgcg ccgggggccg
     9661 cgaattcctt cccaatcatc gatggcgccg aggaggctga atgaaaccat caccaccacg
     9721 ggcagcaaga cagaacgacc gctgaggtgc agacccagta tcgaagccgc attcaataaa
     9781 acggtaaaaa gcaccactgt tcccacgatg acaagcccgc ccatggtggg tgtgcccatt
     9841 ttcgtaatgt ggctttcggg cccttcgacg cggatgatct tacccatctt gaagtgcttc
     9901 agcacgcgca ataatggacc gccccagata actgacagaa taaatcccaa tccgctcagg
     9961 gataaggcta aggcggtctc tttcatcctt ccacctccat cgcttgcaca atgcggtcca
    10021 tacgcaggct gtgagctcct ttgaccagaa ccacatcacc cgggcgcagg gtcaaattca
    10081 gagcttggat gacctcgttt tcatcatcaa taatttcgat aaactctcca gggaggcctg
    10141 ccttttgtgc ggcggtggcg atcagctgcg ctttttcctg aaaggcgatc aacttatcca
    10201 ccacctcagc ggcgcggatg ccaactttgt tgtgaccaag ttgttcgtat tgacccagtt
    10261 cgttcatccc tcccaacaca gcgatcttcc tgccgggaag ttcgttcagc aggtttaacg
    10321 cggccagcat agactctggt gaagcgttgt acgtgtcatc taatatcagg gcaccctggc
    10381 gagttttcac agctacaagg cgcaactggc tggtggcatg ttgcaggcct aaaatgatct
    10441 cctgccaggt caacccttcg ttaagcgcag cagcagctgc agccaggatg gtatggatgg
    10501 aatgccgacc gattaacggc gcctgaaggt taaacgcgtc cgcgccgtgg tggatcttga
    10561 agtgaatacc atcaagccca cgagactcta tctcatccgc ccacaagtct gcctttgagt
    10621 ccaacccata ataaataact tttgcctggg tcaggttcgc catctctctg atgaaggggt
    10681 catcgatatt gaggatggcg ctgcccgtag gggcgggcgg cagggcgcgt acaagttcgc
    10741 tttttccatg ggcgatggtt tcctgcgaac cggcgcgaga tgcgtgtaca gtgccaatat
    10801 tcgtaaggat gccgacctgc ggtttggcaa tttcacacag gctggcaatc tcgccgggtt
    10861 catagaaccc catctccaat accacacggt catagcccgg cccgaggcgc agcagcgtga
    10921 gaggaagacc aacttcgtta ttaaaattgc cctggttttt aagggtgcgg tagcgctgcg
    10981 agagaatttc agcggttagt tctttggtgg tggattttcc gacactgccg gtaatgccaa
    11041 tgacgcgggt ggtgaacttg cgccgccaga atgccgccac ggtttgcagc gctttggtgg
    11101 tgtccttaac gcacaaagca aatggcagcg gcggaaacgc atggtcagct gaaataggct
    11161 ttccaaggtc aacaacaggg atggaagggt cgatctcatg ctgcaccagg gcaaagcgcg
    11221 cgccattggc aaaggcgtgc gatacatatt gatgcccatc agtacggttt cctttaatgg
    11281 cgacaaacag ggaggcagga atggcaagcc gcgaatctac aactccttcg ctgatacctg
    11341 gagttgctcc aatgggcctt acgcctgtca acgcttcaat cacatccgcc agtgtcagca
    11401 taatttcctt cactcttcct tcaggctcat cctgatttca tcaggcggta aattcatcag
    11461 cacgacaatc tcctgcacga cattgctgaa taccggtgct gcgacgatcg aaccccaaat
    11521 ggaggtgctg ggtttttcca accagacgaa aacgataaat tgcggatcat ccaccggtcc
    11581 ccaaccgacg aaggaggcgt tggttttatt gctggaatat tgcccattga cagcaatttc
    11641 tgcggtccct gtctttccgg caacgcgata tccatccacc agtgcggtgg aggcttcttc
    11701 ttcaagggag atagccagca tatcggtgag ggtttttgct gtttctgccg tgatgggtcg
    11761 acctacgacc tggggcgcgg tatcaaattg ctgtccatcc tggatataag atttgagcac
    11821 atgaggtgcc accatttgcc cttcgttggc gattgcggac gccgccatga gcaactgcac
    11881 tggggtaacg gcaacgccct gaccgaatga gttagtcgca aggttgactg tgtaccacat
    11941 tgggtcaccg ggaacactga ggggatagac ttcttcccct gcaaggtcga tgttggtgcg
    12001 atgaccaatc ccgaaacgtt cgagataatc ataaaacctg gttgggccaa gctgcgtggc
    12061 gatccaggaa aggcatacgt tgagcgagtg ctgcatgcag ccgatcatat tctgatttcc
    12121 ccaggcaccg cggtcccagt tgtagatcat ccagccgccc acttcgatct gacccgtatc
    12181 ggtaaagggg gtgtcagatg tgaccacacc agcatcgagg gcggctgcca tggtcaaaat
    12241 tttaaagact gaacctggtt cgtagacctg gttgacagct ttgttgtagg gtacccctgg
    12301 cgggaaaacc ttggtgtttt cccagtaggc gttggggtca agacggggat tgctggccat
    12361 ggccaatatc tcgccgttat tcgggtccat cacgatgatc gtcccgccgg ctgccccatt
    12421 ggtttttacc gccttatcca aaattccttc gaccgtgcgc tgcatctcac ggtctattgt
    12481 gagcaccagg ctggcgccag caggagcggt aggtatctct tgcaactcgt aggggtccag
    12541 gggaatcaca acgttgcgct ttatgcctgc cagcagcgtg ttgtatttct cttcaacccc
    12601 aaaataacca gccccgcctt ctcggtcata ataggtgaaa aacccgagta tgttggctgc
    12661 gaggttgcct tcagggtaga tgcggcgaag gtgcgctgtc caggttaacc cgcgcagcga
    12721 aggcaatgcg gtatccaaac cataagggtt gttattttca aattgtgttt ttaagtcgtt
    12781 aatctctttg atcttgtagg attcgacaaa atccgcgagg gtgacataaa cctgttgacc
    12841 aggaacatac ttcgtattcg ccgcgttgaa aacttcttga taatccaatc ccaatacgtt
    12901 ggcgagcgtc gaagcgatag ccgaggggtt tctaacgaac tgcaactcga taccaacttc
    12961 ataaacctct ttattgcctg ccagcaggtg accgtaacgg tcataaatgt aaccgcgttc
    13021 ggattcgatg gtgcgaagtt cgtaaccgta gtcttttgcc caatcattca ggtagtcggc
    13081 gtgagtgctg gattggatac gaaccatctg caccgcaatg acaactgcaa acacggagag
    13141 ggagatcccg agaaagtggt agcgcatgaa cctttttctc atcagcttcc gtctcccaag
    13201 aaaccagtac ccagagaatt gacaccagtg aagagccagt cccaaagaga ttcgcgatac
    13261 acggatttaa ttaattgccg ggacgtttca ttgatgcctg gaggaggggc ggcgaccatc
    13321 gtcctgcgtc ctgagtatcc tggcacgatg acatagagag catcttcagg ggcaatcttt
    13381 ttaaaaccca tgctcagcgc tctctcttcc atcgcgggtg cggcggtgat gagcgccagg
    13441 gtcgctttac gatgcgcgat ttcgcgttca aggtcctgcc gcttgagatc aagtcggtag
    13501 gcatcgagcc cggcgttggc agccaggccg ctgatgctga gatagacact ggcaactacg
    13561 agaacaacca caaggctcaa cagaaaaaag ccaacagtgc gcgcgtgaat tttccagggc
    13621 aactgcttaa gcgcctggat taaccgcatc ctcttcgcgg ttcttgcttt ccgcctgttg
    13681 gctctatctc gaggttccac catgctcggt atgcctatct cttttctgcc acgcgcaatt
    13741 tcgcgcttct ggcccgaacg ttgctgctta cttctatccc gtcaggggtg atggggtgac
    13801 gggtgacttc aatgagcgaa gctttgtgcc cgcagataca aacaggttgg tctgcggggc
    13861 agatacaatc tctgctttca cgcctgaaaa actgcttgac aatgcggtct tccagcgagt
    13921 gaaaagaaat gaccgcgagt cgaccgccgg gtgccagcga ctctaccgcc tggggaagga
    13981 aaatctgcag cgagtccagc tcatggttta cggcgatgcg cagcgcctgg aaagtccgag
    14041 tggcgggatg aatttcgctt cgtatgccgt agctggcttt ttttatggtt tctgccagct
    14101 gcaaggtgct ggtgaagggt cggttttgaa cgatgacacg cgcgatgtgg cgtgaacgcc
    14161 gttcttcacc gtaacgccag atcaggtctg ccagttcgga ttcagtggag tggttgacca
    14221 gtgtctccgc gctgacgggc tgggacggat cgaagcgcat atccaatggg ccatcgttga
    14281 gaaaagaaaa accgcgttcg ggagaatcga tttgcattga agacacgccc agatcggcga
    14341 cgatgccatc tacgtggtcc cagcctaacg tcagtagcgc gttgcggagc tcggtgtagg
    14401 aggagtgtag caaatgaaag cgctggtgaa aaatggagag gcgctggtga gaaagcgcca
    14461 gcgcctgagg gtcagtgtct aagcctagca actgtccgtc tggcgagctc ttctccagga
    14521 ttccccaggc atgtccagcg gcgccaatgg tcgcatccac gtataacccc gggcttacag
    14581 gacgtaaagc agttataatt tgatggtaaa gaactgagac atgcggtggc ttggccgcgc
    14641 tgtcgtgtgc gtttttcatc agatggtcag gtcaaaactc gagacgagtt ctgctgcaga
    14701 agaaggtcct tcaccctggt cctcctgaat cttgtaggtg tcggggttcc agatttcaat
    14761 gtctttacca ataccgacga tcaaagccgt accatccaac ccagcaattc ggcgtaaggg
    14821 ttgaggaatg ataaaacggc cggccttatc gaactcgatt tgagcggcgg tagagaagat
    14881 atatcggcgt aattggcggg tttgggcgtt tgccatattc atattgttaa tgcgttcata
    14941 gaggagttca aaacggttgg cattcatcac gagcaggttc tggtcaaatc ccttgagaat
    15001 gaacgggttt tctcccagga ggtcgcgaaa agagacaggg aacgttaagc gccctttttc
    15061 gtctatatta tgaagataac aaccggtgaa acccttgaac ataatttcta ccttttttta
    15121 ttatggcgaa cgccggaaag aaatccggcg ctccacttat tcccacttcc taccactttc
    15181 ctccacatta tacagattat tttgtacaaa tgcaagtgtt ttttacgttt ttgatatgca
    15241 attaagataa attggtgcag gttttttgcc aaaaatagac aaggagcaga caatgagatc
    15301 attttttgca cgattttcaa tcctggtggt cgttttactg gggtttacta tgctgaacgg
    15361 gtgtattacg attaatctgg gtggagcaac gcccgactcc ggacaaacac tcgagccagc
    15421 ccccacaaca cctccgccgg ctgctgtgca aactccccgg caagttttaa atgaagagcg
    15481 gctgctgaac gcagattatc tttccccgtt gttccaggta cctattaccc tggttggcgg
    15541 ggtgttttcg ggagtggtgg acggctccga gctgaaagtg actctgcaac cgggtattca
    15601 gtatggagac ctgaatgatg atggggtcga agacgcggcc ttcatcctgg ctgaaagcac
    15661 tggcggcact ggggttttct cctctttggt ggtggtcttc agcgaggcgg ggcgatttgt
    15721 gcaggcgcca ggcgtgatga tcgatgaccg cccggtgatc gagtcgctga ccattgaaaa
    15781 cggggtggtc aagttgcagg cgttggttca ttcatttaat gacacaatgg tcaatcccag
    15841 cacgcgcatc cgcgcagagt actcactgtt ggacggcaag ctggtgcaaa cccggctctc
    15901 ttctgctttc tctggcagcg aggagcacgt gattaccatt gattcaccgc gtgatggtga
    15961 tactataagt ggcagtgtgc gcctgatcgg ttcgatgccg attggaccgt tcgagaacac
    16021 tctcttgttg aggatcactg acctatcagg tggggagctg ctgcatgaag gattcatggt
    16081 gcaaacggaa gacctgggcg ctccggcgac ttttgataac accgtgctca tcccggggat
    16141 tgcccccgga acgcggattt tgttgacgtt gagcgagttg agcatggcgg atggctcgcc
    16201 gattgcactg gattcggtat tgctggtggt gggagaataa ctcgcgactg gcagcatatt
    16261 cgctgataat cccctgtaag atactggagg tcagacgaaa aaaaacaggg cgcgatatgc
    16321 cgcgccctgt tgaagactgt tggcataagt gtcaaccatc aaattcacca accagcatgt
    16381 catttacgta gacaaggtaa tgtccataag gataagcccc cagcggtacg ctggtttcaa
    16441 attcttgaag cacctggata cacacgaccg ctggatcaac aagcgaatac acactgatgg
    16501 cgatatggtt ttccttgtca ggcggggaga tggcgacgcg caatatgtga cagggcgtag
    16561 gcaggctgcc ttttatttga aggttgacct ggatggggtc gctttcaaga atcatgagct
    16621 gggcgttgtc gacgaacgct gcggaacgct gcttcaactc atcaccagga ccaggttcaa
    16681 aagagttggg cgttttcaat ggtgtcaaag aatcaacggg ttggtctggc ttggcgatcg
    16741 taggggccgg aggtgagggg ttttcatcat tgattactgg gcgagccgtt gcggtagaca
    16801 gtggtgaaat aaagtcaggg gtgtcagaca gcgtcggatt gcaggataca agaagcagca
    16861 ttattgagat ggaaaaaatc aaaaagcgca tggtccctcc tggtcaatca agttatagag
    16921 ttgacgcttt ctcagttgcg attgttccgc gttgaggttg tgaggttttc aacaagcgcc
    16981 aggcttatgg ctgtgattga cggtattccc gccagccccc ccaacgggtg atttcctgct
    17041 ggccagaaat aagataaggc tctgcgagca caccaaagac caattcacca ccctgcagct
    17101 cgacgcgccc caggcataac ccgggcggtt cctgttgcag cacggcaacg agcgcgcttg
    17161 ctgagagcga ccatatttcc acgtggatgg cagcgcccaa atgatcatca cgcagcatgg
    17221 cagggtagag atcgccaatc gaccatagac ggtaagtggg tgcggtgaca gtttggcaca
    17281 ggaagaccgc tccgacggag agcaggttgt ggttcagttc caacccgcgc atcagggtgc
    17341 cgttgactgc cagttttact tttctcatta gcgaaatcct aaacatcgct cacacgcggc
    17401 cggtattgca gcgcttcagc caggtggatg gtagagatgt gcgggctgcc agccaggtcg
    17461 gcgatagttc gagccagctt gaggatgcgg tgataggcgc gggcggaaag ctgcagctgg
    17521 ttcatagcgg tgcgcatcag tccgcggcag gattcatcga gagagcagta ctgccgcacc
    17581 tgcgccggat gcatatccgc gttagaggtc aatgacgtat cagcgaaacg ctcgcgttgg
    17641 atttgacgcg cgctctccac ccgggtgcga atgacagcgg agggttcacc caggcgtgcg
    17701 tcgctgagct tctcgtaatc cacgcgcggt acttccacgt gaatatcgat gcggtccatc
    17761 agcgggccgg agatgcgccg ctgatacttg gtgacgatgc ccggcgcgca ggtgcaggcc
    17821 cgggtagggt ctccgtaata accgcacggg caggggttca tggcggcgac gagctgaaaa
    17881 ttggccggga aggtgagcgt gccctgcgcg cggctgatgg taattatttt gtcttccagc
    17941 ggctggcgca gcacttcaag ggtgcggctg ccaaattcgg gaaattcatc gaggaagagc
    18001 actccacggt gggccaatga aatctcgccg gggtgcggcc agttgccacc gcccaccaga
    18061 cctgcgtggc tgatggtatg gtgcggcgcg cggaaggggc ggctcttgag cagtgggata
    18121 ccctcaggca gtgcatctgc cactgaatag atgcgggtaa catcgagggc ttcttcgagc
    18181 gtcatggagg gcaggataga tggcagggca cgggccatca gcgttttgcc tgcgcctggc
    18241 ggaccaatca tcagcaggtt gtgcgcacca gcagcggcga cttcgagggc gcgtttgacg
    18301 tgttcctggc ccttgatctc gcggaaatca gtctgtacca gcgcgctgac ctgttccact
    18361 gccaaggcag gctgtgggtc gatgatcacc tgaccaacaa gatgctggaa aaggtcagcc
    18421 agagaactga ccgggatgac ttccaggtcg gggaagatag cggcttccgg cgcgtcctcc
    18481 gcggggacat aaacgcgctt ataaccctgg gcgcgggcga cggcggccat gggcagcact
    18541 ccgcggatgt ggcgcacgct gccatccagc gagagttcgc ccatcaccag ggcgtcttgc
    18601 aggcactgcg gaggtagttg gcggttggcg atgaggatgc ccagcgcgat gggcagatcg
    18661 taggctgagc cttctttgcg cacggtggct ggagccaggt tgacgcgtac gcgtttgcgc
    18721 ggatactcca gcccagtgtt gcgaatggcg gattggacgc gctcgcggct ttcctgcacc
    18781 gagacatccg gtaagccaac gatgatgatg ggcggtttga tgccgggtgt gagatcgacc
    18841 tcaacatcca ccacgacccc atccaaaccg atgacagcgc aggcgttcac tttggcaagc
    18901 atggattaag tgtagaagtg gagagggaaa aagtcaatca tctttttact ggttaatccc
    18961 ttttcatcag cccttgtgaa aggaggattg ccctggcagg cggtcttggc aatatctcga
    19021 caagatgcga accacaggaa aaatcgcgta gcctgatatg gtgaaaagta tgtgagtgag
    19081 gggaaaccag gacacccttg cctgagttgg aaaggctagc gggctggctg actgaccagc
    19141 tggtggtcgt tttatgtagt atttattatg cagctgctgc cttaaaagat atctgcacag
    19201 ttggactggc agcaagggta gccgccactc ggtcaaccga gcagtttcct cacccgccgc
    19261 agcatgcgcc ggtcatcttt cagtagaccg gcggcgggca ggcgttcgag cagcagcagc
    19321 cagttgggat tggcttgaaa cacacgtttg agaatgggca gggcttcctg ctccctgccg
    19381 ctatccagca aggtgaccgc ctgccagaag ggaagttcat gcaaatgcgg agcgagcgcg
    19441 gcagcctgct cgtaggcgct aaaagcctga tcgataagcc cctggctgag cagctcatca
    19501 ccgcggttca tatgttcata agcatgttga acggtgagca ggcggcgtaa ttcaaccagg
    19561 ggttcggaat ggtcttcaac acgcaggtct accagtaccc ctttccatgg ttcgttatgg
    19621 ctctctcctg ataccaccag taaagcggcg gattgttgac cgcgaatatc tcctccctga
    19681 gcctgggcag catccagcgc ggcgagcagg cggtctgcaa gcgaaccctt cgccgcttca
    19741 tatgcggcgg ccatcgccgg ccagaccgta ttacgcagca tcatattcgc ttgcacagaa
    19801 tactggtaac cagtttggtg accagcttcg gcgatgcaac gttgccctgt atgcaccgct
    19861 actgtgcctt ttccatctac catggccacc tggcgcactt cacggttggc gtcttttgcc
    19921 agcaggtttg caagaacaac ggaggctggt ttgccttcgc gcatcgcgct caatcccagc
    19981 gggccatagc tgacttcagc catggattgt gtagcgatgg cgcctacacc cggctctgcc
    20041 caggcaacga tggagcccgt tgaaaaccaa tgcgattgca cagcaacgcc caactcaccg
    20101 ttcttggggt ctcgggcgac aatggaaaag gtgtgcgcga aaggaaaacg tctctctggc
    20161 atggaaactc cgattgcggc tggttctgat tcagcccatt tcctggtgaa tatccgcgcg
    20221 gcaccatcgc gcgaatatcg cggtggagag gaagcgtttc gctgaccgct ccagcaagcc
    20281 aatctctcca gcctgggtaa tgcctttaaa cctggcggtc atttcgagaa tggcgttgta
    20341 cattgttgcg gcagaagcct taacggcgta cgcggtgagc tgaacaaaaa gcgggtcagg
    20401 ggagaggatg gcgcggcagg cggcgagcag gtctggcagc agcttgtaaa aatcccatat
    20461 ttctcctttg ggtccacgcc cgaacttggg aggatcaaga atgagagcat cgtagcaact
    20521 gccgcggcgt tgttcgcgct gaacaaactt aagcgcatca tccaagatcc aacgaatcgg
    20581 gagtgaatca agcctgctga gggtttggtt ttcacgtgcc catgtgataa ccttgcgcga
    20641 ggcgtccaga tgggtcacct gtgcgccagc cgccgccgct gagagagaag cgatgcccgt
    20701 gtagccgaag aggaccagca cgcgcggctt cccgcctgac aggtggatga ctttttctgc
    20761 ccagtcccat tggcaggatt gttcggggaa gacacccagg tggcgggagg aagaggtttt
    20821 aacccaaaaa gcgagttgcc catagctcag cttccattga tcgggcattt tcttacggaa
    20881 ttcccaatga ccaccatttt cttcgggggc gggtttataa agcgcatcgg ctgcccgcca
    20941 ctcattttct ggtaaggctc gttgccaaac tgcttctgct tcgggacgga tcaagcgata
    21001 cgctccgaag cgttccagct tcatcccggc gccgctatcc aacagttcat aatcctgcca
    21061 gctggtcgcc gctaacaagc tcaggtcagg aatcgtcatc gggtgttcca tataaaaaag
    21121 tatatcagac ttgaccattt gagcgcgtat caatgcgggg tggactataa tccatcattg
    21181 accaagaaag gagcagcgaa acgatcatgt tccaagcacc tgaacgcagg aataccgata
    21241 gtgttaaatg gggattgttc accgaagacg tattgccctt atgggtggcg gacatggatt
    21301 ttacctcgcc cccggtcgtt attgaagcgt tgcagcaacg ggtaagccat ggcgttttcg
    21361 gctacgcact ggaatcacaa gagcttaagg atcttattgt gcagagaatg agccgattgc
    21421 accagtggag aatttcaccc gaagatatcc tgttggtgcc gggagtggta tccgggttta
    21481 acctggtttg tcaggcggta gtgcacccgg gagaatcgat actgctgcaa ccgccggttt
    21541 acccgccatt tttcgaagcg ccggtcaaag cccaagcgcg cacggtatat aacaatttgc
    21601 agcctggcgc agatggacag taccgggttg acctggatgc gttcgccaag tcaattgaaa
    21661 aggatacgaa ggctttcttg ctgtgcaatc cgcataatcc attgggcaga gtattcaccc
    21721 gcgaagaact gcttgggatg gctggcgagt gtttgcgtca cgagatgatt atcattgctg
    21781 atgaaatcca ctgcgacctg gtgtacaaag ggtaccagca tattccgatc gcttcgctgg
    21841 atgatgagat tgctgccagc acggtgacac tcatcgcacc aagcaagact ttcaatatcg
    21901 ccgggttgga ttgttcgatc ctaatttgcc agaacgaaga gttacgcaag cgtattctcc
    21961 attccaaacg cggactgatg ggcggggtga acctgctggg aatgactgcg gctgcagcag
    22021 cctatcgtga aggggcttcc tggctgagcg aggtgcttgc cttcctcgag agcaaccgag
    22081 agcacttgat ggcttacatt gagcacaacc tgccacgcat acatatggtc aaacctgaag
    22141 cgacgtacct tgcctggttg gattgccggg agctggaact cgcaccaagc ccgcgcgaat
    22201 attttttcca acgtgcccgg gtagccctca atgacggtcg agattttgga gaacccggta
    22261 ccggctttgt gcgcttgaac ttcggttgtt cgcgtgagac actggataac gcgctcgaac
    22321 gaatgtgtca ctcactctct taaaagaaaa aagcagagtc gcaaggctct gcttttaagt
    22381 ggtattcgat ttttgattat tctacaggtt taacttcggc agcctgcaaa cctttaggac
    22441 ctttctcgat ggagaattcc acctgttgcc cttccttaag aactttgtat ccctccattt
    22501 ctattgcgga aaaatgcacg aagacatctt cggcgttttc tcgcccgatg aaaccgtacc
    22561 cctttgcggg gttaaaccac ttgacagttc caacaaaacg ttcagccatc ctaatactct
    22621 ccatcttaaa tagtgttagc ggaagggcga actcccctcg gcgcctttga tatggtgaat
    22681 ttaccatacg cataaactgg tgtcaagcaa atgaggagca gcaaaatttt gtgcgaagcg
    22741 ggtggcatat aataaagcta tgaggaatga acgtaaatta tggttgctgt tctttttgct
    22801 cctgccattg ggatttatgg ctttcccaaa ggcaggagca gcgcctgaaa accttaccct
    22861 gtctttgcaa aacgaccggg cagcgcttgt caatggagat gcgcagatgg aactcacggc
    22921 gattcaacag gcgctggaat tccagacctg gcggggagac ttgtggcagc gcgcaggacg
    22981 attatacatg gatcaagggg attaccagaa cgcgctgcat tcgtttgaga aggctcaaga
    23041 acttggtcag cttgaaccgt tagggcaggt ttggctatct gacatgttga tcgccaacgg
    23101 ttatagcgac gtagcaaaat atgtattgca atccatccat acagacgatg tttttatcct
    23161 gcttcaagcc gctgctctga tggcgccgtt aaacgatcac gagggattgc gccaactgct
    23221 gcaaaaggcg tacgcgtctg aaccaaccaa cagtgaagtg aattaccaga tgggtatcta
    23281 tttaatgaca gaagacccag gccgtgcggt tgcgtacttt gaactggcta aaagagacca
    23341 gtcccgtaag gcggctgcgg cttacctggt agagaacatt caacaatatg gcgctcttga
    23401 ggaccacgga gaatggtatc tctacgctgg gcaagcgctg gcgcggatta atgaatggaa
    23461 cgcggctttt caagccttct ccaaagcggt gcagtcgctg ccagataatg ccatcgcact
    23521 ggcgttgtta ggagagtccc agttccaact tggattggat ggacggcaaa acctcgagaa
    23581 agcctgggag ctggaccctg aaggagaaat aaccaacgga gtgttaggga tctattacca
    23641 gcgccagggc aacctggaaa aagcgctgga gcacctgcga attgcacaaa aatcaaaccc
    23701 gtcggctgct atatggattc tcgaaagcgg cagaaccctg gcggcgatgg gggacctgga
    23761 aaaagcgctg acagaatttt ctcttgctgt taccgtggat gagaagaacg cggatttatg
    23821 gcgggtgttg gcggagttct gcgttttgca tcgttatcag ttagaagacg ttgggttgcc
    23881 ggctgctcgg caagcattga cgctggagcc agataacccg gtattgatgg acttgctggg
    23941 aacagtctac ctgcaattgg ctgacctgga tagcgcggaa cggtttttcc tgcgagccct
    24001 ggagcgagac cctgaacaag cagccatcct gatacacctg ggacagctca acttgataag
    24061 agaagagaaa gagatagcct ttggctatct tcgccgggct gaagcatctg cccgcgattg
    24121 gcgtctgcgc gacctggcaa accgcctgtt aagagaaaat ggcgcgcagt aaatggggaa
    24181 ggtataatat tgcagtcagg aacaggcaaa agtgaacaaa ccgactcacc ttttttttcg
    24241 ttttttactc ttttgctgcg cattgaccct gacaggctgc gagaagatcg gcattcactt
    24301 ccctcaatcc ggggctgtac ccccgggcgg actgttattc gaagatgatt tttcccaccc
    24361 gcccaatggc tggggatcga tggaaacgag aggaggggag attgaattta tctttgatgg
    24421 gatgatgatt tccatcttcc tcccgaattt tatgtactgg tctgtgaatg gaaaggaatt
    24481 cagcgatgtg aaagttaagg tggatgctgt gctggcggat ggtccacagg atgatacctt
    24541 tggcgtcatc tgccgctttc aggacgaccg taacttttac ggatttgcct tgagccatga
    24601 cggctattac ggggttttta aatatgtgga tgggaaaatg aacgtgctat caaatgaagg
    24661 gaatctggga ttcagcgaag ccatccgcac gggtggcgtg gtcaatcacc tggaggcgac
    24721 ctgcgagcga aa
//
LOCUS       DAOTLW010000032        20955 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_2914_length_20955_cov_10.346842, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000032 DAOTLW010000000
VERSION     DAOTLW010000032.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 20955)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 20955)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20955
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_2914_length_20955_cov_10.346842"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..408)
                     /locus_tag="P5516_06250"
     CDS             complement(<1..408)
                     /locus_tag="P5516_06250"
                     /inference="COORDINATES: protein motif:HMM:NF012893.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-transporting P-type ATPase"
                     /protein_id="HRT92076.1"
                     /translation="MSDIKTPQQKTVDNEDISISWHSLSADEALQRLESFRAEGLTSA
                     EAEHRRKEYGLNQLEEKPRRTFIQMVIEQLRSFVILVLMGAAILSAVLGEYIDAGAIV
                     AIVLLNTILGVVQESRAEEALAALRRMAAPESQV"
     gene            complement(430..1686)
                     /gene="glgC"
                     /locus_tag="P5516_06255"
     CDS             complement(430..1686)
                     /gene="glgC"
                     /locus_tag="P5516_06255"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HRT92077.1"
                     /translation="MGATDDILAVILGGGRGARLYPLTKERSKPAVPMAGKYRLIDIP
                     ISNCINSGIYHIAVLTQFNSVSLHRHISRTYNFDAFHSGWVQIWAAEQTLENTNWYQG
                     TADAVRKQLLEISSARTSHVLILAGDHLYRMDYAKMAEFHLEKDADITVAVQPVSTVD
                     CQRFGILKRTPNCQITDFHEKPTDPEVLKDIISRDDPARPYLGSMGIYLFKTKVLQEL
                     LENERHDDFGSHVIPTAIRSHRVYGYDFHGFWEDIGTIRSFYQANLALTDLNPPFNFY
                     DQNFPIYSHPRFLPGSNIEDTIMKNVLIAEGCRIEKATLIHSIIGLRSQIRGGTTIID
                     SILMGNDYYEKPGSPEAFAGALEIGFDCYIEGAIVDKNARINKGTIIKPFPVGTDIDG
                     DGWLVRDGIVIIPKSAVIPPGTEITP"
     gene            complement(1824..2483)
                     /locus_tag="P5516_06260"
     CDS             complement(1824..2483)
                     /locus_tag="P5516_06260"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HRT92078.1"
                     /translation="MIPIGDDNSQRRLVPVVTWILVILNVAVFLLELMGGDVFIMMWS
                     FIPARFMQDPAGNMITIFTSMFMHAGWFHLIGNMLYLIIFGDNVEDRFGHVKFLLFYV
                     LCGFIAMLAQLIAAPYVTIPNLGASGAIAGVLGAYLVLFPRRRVRVLLGWWIVHLPAL
                     IVIGGWILIQVLSSLGSLAGSASAAGGVAYMAHIGGFLSGLVLSFFFRNRVRALPPSY
                     M"
     gene            complement(2700..5225)
                     /locus_tag="P5516_06265"
     CDS             complement(2700..5225)
                     /locus_tag="P5516_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HRT92079.1"
                     /translation="MERFTQRARRVLSLAHQEAERMRHTAINTEHILLGLIEEEGGIA
                     GHALRELGLETARVQEMVERLAPIGLQESSALELSVGAQQALEFSIEEARKLGHQYIG
                     TEHLLLGLTRVTDGLALEVLKKLGVSAEQVRRQTRRVMEESASGPASAGGAPLARREE
                     KKEAKTPLVDQLAVDLTTLAEEGKLDPVIGRQQEIERVIQILARRNKNNPALIGEPGV
                     GKTAIVEGLAQRIIEGDVPAPLIGKRVLQLDVGSLVAGTMYRGQFEERLKRVIDELKA
                     SRSILFIDELHMLVGAGSAGSAVDAANILKPALSRGELQVVGATTLDEYRKHIETDAA
                     LERRFQPILVVEPTVEETIQILKGIRKAYEEHHRLVISDEALEAAAHLSSRYVTERFL
                     PDKAIDLIDEASSRVRMYKSPAAQTFKELMLELKDVKAHHSEAVQSGRSDDAMELSNK
                     EADLRGRIEKLRTGWDRTTSPVVNAEDIAELVSMWTGVPVVQLATEESERLLRMEEEL
                     QKTIIGQEEAIKTISKAVRRGRAGLKDPRRPIGSFMFLGPTGVGKTALTRALALFLFG
                     SEDALIQIDMSEFMERHNVSRLVGAPPGYVGYDEAGQLTESIRRHPYSIVVFDEMEKA
                     HPETHNMLLQIMEEGQLSDARGRKVDFRNAIIVMTSNIGSDLIRRQGSLGFTLQRDEK
                     ENEAKNYEDMRKKLLDSLRKVFRPEFINRLDGVIVFHPLSREHIRSIVHLELDKVAER
                     MKEHNITLRASAAALEHLSAEGYNPEMGARPLRRVIQDSVEDRLSDAVLSHEFNAGDA
                     VIVDVTKEGEIILKRNRKRAVALKPGKSAEGETQAEEPERVQV"
     gene            complement(5494..6378)
                     /locus_tag="P5516_06270"
     CDS             complement(5494..6378)
                     /locus_tag="P5516_06270"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HRT92080.1"
                     /translation="MVATLEGQRQNAPRALAAHVDTLGAMVKNIKPSGRLSLSKIGGF
                     AWNTVEGEGCTVFTRSGRRVRGSVLVTAASSHVYGAKTAELKRDDDTMEVRLDEKAAS
                     VEEVKSLGIDVGDYIAFDPRVELTNGFVRSRHLDDKAAVACLVEALRALRSEGLKPAQ
                     TTYFFISNFEEVGHGAANGLPAEAGELVVVDMAAVGDGQNSDEFHATLCVKDSSGPYH
                     HGLSNHLRKLADNHGIPYKVDIYPYYGSDGSAFWRAGGEAAVALIGPGVDASHNYERT
                     HTDALTATANWILAYLLD"
     gene            complement(6552..6974)
                     /locus_tag="P5516_06275"
     CDS             complement(6552..6974)
                     /locus_tag="P5516_06275"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF024675.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HRT92081.1"
                     /translation="MNPFKYHYPIDVRFGDLDAFWHVNNARFLVYLEDARSRYLQEMG
                     LLDGKALWQLPIIVGDIHIRYRQPIELGHKVVVSMGATRISAKTVTFEYQITGEGGTP
                     LHATAESIMVCYDYETKKTVPLSDELRRKFSEREGREL"
     gene            7129..7530
                     /locus_tag="P5516_06280"
     CDS             7129..7530
                     /locus_tag="P5516_06280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HRT92082.1"
                     /translation="MKITRQADYALRAMVYLSQKSDSPTSTKEIAKAQGIPPSFLAKI
                     ISQLSLKKLIIASRGANGGVALARPASNISLLEVIEAIDGEIALNQCTVSIGNCDRAE
                     DCPLHIIWQETQDELVTKLRNTTFDRFSKKN"
     gene            7568..7825
                     /locus_tag="P5516_06285"
     CDS             7568..7825
                     /locus_tag="P5516_06285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92083.1"
                     /translation="MATGTPINRQMVIVLKNGIIAIDWGDGLYQDIRTGDFIPVLETD
                     YSHHILNEELDWLIKIGRVISYDKNTVQSQSLPERPQRTID"
     gene            7893..9059
                     /locus_tag="P5516_06290"
     CDS             7893..9059
                     /locus_tag="P5516_06290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92084.1"
                     /translation="MLTHKTLLQRIAFFTGLRLTLNTSIRMIYPYLAVFAVALQRDIG
                     AISLVLAASMFTSAVGPFIAPIADRRGRKVGMLIGMGIFLAGTFSASLFPSYFTFFLA
                     IMVGNLGNNIFLPSMQAYLGDLVPYNKRGLYLAITELSWALSFILLVPLAGLIITNTY
                     WYGPFIALSVLGSVMTILLWVLIPADHPANPEPLAIFSDIKKVILYPPALLSILMGTL
                     FVAGNELVNVVFGVWIKDSFGLQIAALGAASIVIGLSELGGEGVSALLTDRLGKERTI
                     ALSLILNSLWVITLPWLGKTTSGAMVWLFFFYLTFEVAIVSTLPLMTEVTPATRATLL
                     SLFVAAISLGRSLADVLAPLLFRGGFLVNVLVCVGFNLLSILALSRIRLPRNAT"
     assembly_gap    9178..9277
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <9278..10132
                     /locus_tag="P5516_06295"
     CDS             <9278..10132
                     /locus_tag="P5516_06295"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92085.1"
                     /translation="LTFLGMFVYLSSTQAYISDHVRAGWRGTALGLVETGWGVSFILG
                     MPILGWLIDRYGWKAPFPFTAVLGGIAFLLVWILVPSHKPAQAPSRSVLQSIWEVLKV
                     PAARWGLLMSLMLISSNEVINLVFGVWLEVSFGLKLAALGAASAVIGISEISGESIGG
                     VFSDRLGRARSIMLGIGLMLVVAAALPFIGGSQAGALIGLFFFYLSYEFTFVSTLPFM
                     TELVPQFRGTLLGANVASLSLGRMIGNLVAPFVFTIGFWANALAAVFFILVAFWALLQ
                     AKKPRPAE"
     gene            10184..11437
                     /gene="hutI"
                     /locus_tag="P5516_06300"
     CDS             10184..11437
                     /gene="hutI"
                     /locus_tag="P5516_06300"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HRT92086.1"
                     /translation="MLLHSASQLLTLAGGPRRGPNPVDLGLILDGAVLVRDGLIADVG
                     STQELLARYPNEQRLDARGKAVLPGFIDPHTHLVWAGDRAAEFEMRLQGKTYIEIMAA
                     GGGINATVRATRGATAEELYRQTRERADAMFRNGTTTAEAKTGYGLELQAELAQLETL
                     VRLDEEGPLEIVPTFMPAHAVPPEFKGREDDYTRLVCERMLPMSAAWWKKRCPGKRLP
                     FVDVFCETGAFNLAQTRRILTSARDLGFPFRAHVDEFENLGGAALAVELGAVSIEHAV
                     KTSAIEIQALGKSETVVVSLPCTPFGLAEPHYTPARQMLDAGAILALATDLNPGTAWC
                     ENMQFVIALACRYLKLTPAEALAAATINAARAIRCEERLGSVEVGKQADLLILSVADF
                     RQLAYRFGGSLVEAVIKRGHLTRVK"
     gene            11467..12102
                     /locus_tag="P5516_06305"
     CDS             11467..12102
                     /locus_tag="P5516_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014069.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HRT92087.1"
                     /translation="MLTLEKAREWYIHTDPVHDFSHIERVYRMAQRLAKLEGADPEIV
                     NAAALLHDAEGTTPGSDARREHHLRSADFAATILKQEGWNEERIRAVQHCIRAHRYRD
                     DREPPQTIEAKVIFDADKLDVLGAVGAVRAVVYAALAGTPFYAAPSSQFLETGVELPG
                     ELHSAYHEYLFKLRHVEKRLYTQTAREIARQRRKYLDKFFIQLIAEYKGER"
     gene            12102..12893
                     /locus_tag="P5516_06310"
     CDS             12102..12893
                     /locus_tag="P5516_06310"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012673.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HRT92088.1"
                     /translation="MTSPQQPQLEQVIQWAKEAGDIAHAGLSQKFDVALKGRSDLVTE
                     IDYRCEQHLINAIRSAYPAHAILSEEAGEVNGETGDRWFIDPLDGTVNYAHHIPFYAV
                     CLAWQSGARLQLGVVYDPAHDECFYAERGRGAWLNGERLAVNTTSDLERSLHATAFAR
                     QDEQLFQRNLQYFAHLSRHSFGVRRIGCAALELCYVACGRTDAYWEQGINAWDIAAAA
                     LIVEEAGAVVTGLDGDLDFFKPPYALLASTPLLHPQLVKLFAQMQ"
     gene            12966..14735
                     /locus_tag="P5516_06315"
     CDS             12966..14735
                     /locus_tag="P5516_06315"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HRT92089.1"
                     /translation="MTKTYTQKGWSLASLYPSHDGPEIQSAFKDLETQTAEFEKKRPL
                     LNPSISQKELLKVIQALESLYRIAYRLSGYAELWFSADTQNQSALGMVMRTEQLVSEI
                     ANRTLFFSLWWKDLPDETASPLMEGIGDYRYWLEEMRHFKPHTLSEPEEKIINTKNVT
                     GVSAFSTLYDALTNRYVFKVEVDGEVKELTRGELMSLVRSPDPDLRARAYQELYRVYG
                     DDAPILGLMYQTIVRDWRNEEVTLRKHKTPISARNLANDLPDEVIETLLETCRRNTGV
                     FGRFFKLKARLLGMDKLRRYDIYAPVAKAEKPYAYEKAVAKVLESFSQFDPRFAQMAE
                     RVFADDHLDSEVRKGKRGGAFCSTINPGITPWVLLNYQGKADDVATMAHELGHAIHSM
                     LAEHHSFFTQHANLPLAETASTFGEMMLVDQLLAEESDPSVRRDLLFRQVDDAYATIQ
                     RQAFFAMFEKQAHQMVVDGASVDEISEAYLENLRTQFGDAVDVAEEFKYEWVSIPHIY
                     QVPFYVYAYAFGQLLVLALYKRFKAEGETFKPRYIRLLSAGGSEAPMKLLDEAGVDVR
                     QAAFWQGGFDVLSEMVDKLEKLA"
     gene            15027..15662
                     /locus_tag="P5516_06320"
     CDS             15027..15662
                     /locus_tag="P5516_06320"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013843.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRT92090.1"
                     /translation="MYFAIEGVIGVGKTTLARLLQPQFEADLVLEVFEENPFLSNFYT
                     DRARYAFQTQIFFLLSRYHQQRRNIGERLGQAKNLISDYTFEKDSLFARINLTGDELD
                     MYFKVQEALAEKIIPADLVVYLRASTDTLMKRIALRDRPYERQMEREYIDLLNRVYDD
                     FYISSPQVSHVLTIETDELNIIERPDDLAFIRNRIREELRLAPYQPVLPIE"
     gene            15674..16666
                     /locus_tag="P5516_06325"
     CDS             15674..16666
                     /locus_tag="P5516_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92091.1"
                     /translation="MRITRELLLKHARENSAALTAKDRSIICIYISGSLLQEDPFIGG
                     ITDIDLICVHNQPVARRREVLRLSHEVNLDLAHYEQEEFEPARQLRHNAWIGGWFENV
                     PIVLYDALRWYDFTRASATAQFWRPEYVASRARSFLNPARKTWNDLEEGNIPQGIKRV
                     TAYLGALRDTANAVAVFSGAPLAERRLLMELPARAAAAGLSGLFADFVSLFTSSEVSD
                     ENFTAWVSSYVGIFEALKQSTTAPVSLSPYRRGYYEKALNALYPHSPAAAVWLMVYTW
                     TKAAAALPRTEQPYKDWQELCRQLDLESRDLPTRLELFDAVLDTVEEAVDHLSA"
     gene            17167..17433
                     /locus_tag="P5516_06330"
     CDS             17167..17433
                     /locus_tag="P5516_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage V sporulation protein S"
                     /protein_id="HRT92092.1"
                     /translation="MADIIKVKANSRTAAVAGAIAGVMREHKHVDIQAIGAGAVNQAL
                     KALVLAKGYLAEEGIEIICSPEFVDVDIDGKVRTAIKIVVDPKN"
     gene            complement(17476..18135)
                     /locus_tag="P5516_06335"
     CDS             complement(17476..18135)
                     /locus_tag="P5516_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRT92093.1"
                     /translation="MKKFIAVAGNIGVGKSTLVEMISERLECMPFYEPVAENPYLSDF
                     YKDMPAWSFHSQIFFLTHRLRIHQELVRTPGSVIQDRSIYEDAEVFAQNLFLQGHISE
                     RDYATYRSLYRTLAEYLPPPDLVIYLRASVPTLLKRIALRNRDYERSIEPAYLDHLNS
                     LYENWITHFTLCPVLTVPADELDYVAHTQHLELIISKVKEKLTGKEEVIFTREEANTP
                     D"
     gene            18497..19027
                     /locus_tag="P5516_06340"
     CDS             18497..19027
                     /locus_tag="P5516_06340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92094.1"
                     /translation="MKKIWVFLVIGGVLALALGAASVVNAQAPVNTPQVEQGGGQGGF
                     GARAAGCGERGENEEVQKLMLNAWSDALGISVEELSNRKEAGENLAQIALSTGRTFEE
                     FRALRVSVHTSVAEQALKDGIIDQAQYQCHLKAVERQMHGRGNGNRPADGHGFTTRGR
                     GDRARGHHCGGRSANP"
     gene            18984..19838
                     /locus_tag="P5516_06345"
     CDS             18984..19838
                     /locus_tag="P5516_06345"
                     /inference="COORDINATES: protein motif:HMM:NF015549.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HRT92095.1"
                     /translation="MPGDIIAGVAPPTPKRCSTKSQTVQGCQYGSPAVLEGIIRRNSP
                     GSTMPQDKTLLPSLSKPLARLAPALPVVLGYLPIGFAFGVLAAAAGMGAFNSLLMSLL
                     VFAGSAQLVAVELVAQGLTPWSIVATTFIINLRHLLMSASLSPYMQEWRPAQVAGFTF
                     ELTDETFGVHSLRFERGETRAILALLINLVCHLAWVAGTLLGMLAGGLIRDVRPLGLD
                     YALPAMFIALLVMQIRGRVYLVVALMAAGLSLLVWSAGFTQWNVIIAAVLAAAAGAGA
                     ERWQKQTS"
     gene            19817..20143
                     /locus_tag="P5516_06350"
     CDS             19817..20143
                     /locus_tag="P5516_06350"
                     /inference="COORDINATES: protein motif:HMM:NF017273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HRT92096.1"
                     /translation="MAETDLLITVLGMALVTYLPRLLPAWLLRGRALPPFITAWLRYI
                     PAAVLAALLLPSLLVEGGRLNLTWDNLYLWAALPAALAAWKWKSLFGTVLVGMALVAL
                     ARLLGW"
     gene            20196..20268
                     /locus_tag="P5516_06355"
     tRNA            20196..20268
                     /locus_tag="P5516_06355"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:20229..20231,aa:Asn,seq:gtt)
BASE COUNT         4561 a         5860 c         5552 g         4882 t
ORIGIN      
        1 cacctggctt tcaggcgctg ccatgcgtcg aagcgcggcc agggcttcct ctgcccgtga
       61 ctcctggaca acacccagga tggtattaag cagcacgatg gcaacaatcg ccccggcgtc
      121 aatatattca cccagcacag cagaaaggat ggccgcgccc atgagtacga gaataacgaa
      181 actcctcaac tgctcgatca ccatttgtat aaaagtgcgc cggggttttt cttccaactg
      241 gttcaagcca tactctttac gcctgtgttc tgcttcggcg ctggtaagcc cctccgccct
      301 gaacgattcc aacctctgca gggcttcatc cgcactcaaa gagtgccaac tgatggaaat
      361 atcttcattg tcgacggttt tctgttgtgg ggttttaata tcgctcattg gttcttcttt
      421 agcggaaaat tacggtgtga tttccgttcc gggcggtata acagccgatt tggggatgat
      481 cacaatccca tccctcacca gccagccgtc accatcgata tctgtgccga cagggaaggg
      541 tttgatgatc gtgcctttgt tgatgcgcgc gttcttatca acgatggcgc cttcaatgta
      601 acaatcaaac ccaatttcca gggcgcccgc gaatgcctcg ggtgaacccg gtttttcgta
      661 gtagtcattg cccatcaaga tgctatctat tatagttgtt ccgccgcgaa tctggctgcg
      721 taaaccgatg atggaatgga taagtgtggc cttttcgatc cggcaacctt cggcaatcag
      781 cacgttcttc ataatggtgt cttcgatatt cgacccgggc aaaaaacgcg ggtggctgta
      841 tatgggaaaa ttctggtcgt aaaaattgaa tggcgggttg agatcagtaa gcgccaggtt
      901 ggcctggtag aaagaacgaa tcgttccaat atcttcccag aaaccatgaa aatcataccc
      961 gtacacacgg tgagacctga tggctgttgg aataacgtga gaaccaaagt cgtcatgacg
     1021 ctcattttcc agcagttctt gcagaacttt cgtcttgaac aaatagatac ccatggagcc
     1081 caggtatggg cgcgctgggt catcgcggct gatgatgtct ttcaaaacct ctgggtcggt
     1141 tggtttttcg tgaaaatcag tgatctggca gttgggcgta cgtttgagaa tgccaaaccg
     1201 ctgacagtcg accgtggaga ctggttgcac cgcaacggtg atatcggcat ctttctcaag
     1261 atggaactcg gccattttgg catagtccat gcggtacagg tggtctcccg cgaggatgag
     1321 cacgtgcgag gtgcgcgcgg aactgatttc gagcagctgc ttgcgcaccg cgtctgccgt
     1381 gccctggtac cagttggtat tttccagggt ctgttccgcc gcccatattt gcacccagcc
     1441 agagtggaag gcatcgaagt tataagtgcg agaaatatgg cggtgcagcg agaccgagtt
     1501 gaactgggtg aggacggcga tgtggtatat gcccgaattg atgcagttac tgatagggat
     1561 atcaatgaga cgatacttac ccgccatcgg caccgcgggc tttgaacgtt ctttggtcaa
     1621 gggatacagg cgggcacccc gtcccccgcc caggatcacc gcgagtatgt catccgtcgc
     1681 tcccattaga aaccctcctg gttaaaaaga attacctgaa agacacgtgg gtaaaacaag
     1741 catactggag aaaattctga ccgtcaatca tcatgccggg cttattgacc atcctccgca
     1801 ttcgtcatat gagtggacag gttttacata tatgagggcg gtaaagcgcg cacacggttg
     1861 cgaaagaaga aggacaatac cagcccgcta agaaaaccgc cgatgtgcgc catgtaagcc
     1921 accccaccgg ctgcgctggc agaacctgcc aggctgccta agctgctcag cacctggatc
     1981 agtatccagc cgccgatgac gatgagcgcc ggcagatgca cgatccacca acccaagagc
     2041 acacgcaccc gccgcctggg aaagagcacc agatacgcgc ctaatacccc ggcaatggcc
     2101 cctgaggctc ccaggtttgg gatggtcaca taaggcgccg cgatgagctg ggcgagcatg
     2161 gcaataaagc cgcataatac gtaaaacaac agaaacttga catgaccgaa gcgatcctca
     2221 acattatctc caaaaatgat gaggtaaagc atgttaccga ttaaatggaa ccagccggca
     2281 tgcataaaca tcgaagtaaa gatggtgatc atattccccg cggggtcttg cataaaacgc
     2341 gcggggatga acgaccacat catgatgaac acatctccac ccatcaactc gagcaggaag
     2401 acagccacat ttagaatcac cagtatccag gtgaccaccg ggacaagcct gcgctgagaa
     2461 ttatcgtcgc caattggaat catatctgcc ctcgaggtgc tcttttttta gaaagtatag
     2521 atgttttccc cccgccgcgc aatggtaaga aacctcgcct atcctgattg ttgcgcatcg
     2581 agcggtgggt ggcaagccca aaacgttatg cgagagccgg atgaaaaaca ccagcccaaa
     2641 aacagctggt gtttgattaa ggaagcactt gaagaatgcc tggggcgatc gtctctcgtt
     2701 taaacctgca cacgctccgg ttcttccgct tgtgtttcac cttctgcgct tttaccaggt
     2761 tttagtgcaa ctgcgcgctt gcggttgcgt ttgaggatga tttctccctc cttggttaca
     2821 tcaacgatca ccgcgtcacc agcgttgaac tcgtgcgaaa gcaccgcgtc ggataatcta
     2881 tcttccacgc tgtcctggat aacccggcgc agcggccgcg cacccatctc ggggttataa
     2941 ccctccgcag agagatgctc cagggctgcg gcagaagccc tgagggtgat attgtgttct
     3001 ttcatacgtt cagccacttt atcaagttcc aggtgaacaa tgctgcggat gtgctcacgg
     3061 ctgagcgggt ggaaaacgat aacgccatcc aggcgattaa taaactcagg tctgaagacc
     3121 ttgcgcagcg aatccagcaa ttttttacgc atatcttcgt aatttttggc ttcgttttct
     3181 ttttcatccc gctgcagggt gaagcccagc gagccctggc ggcgaatgag gtcagagccg
     3241 atattggagg tcatcacgat gatggcgttg cggaagtcta ccttgcgtcc gcgcgcgtca
     3301 cttaactgac cctcttccat gatttgcagc agcatattgt gcgtttcagg gtgcgctttt
     3361 tccatctcat caaaaaccac gatggaatag gggtggcggc ggatagattc agttaactgc
     3421 ccggcttcgt cgtagcccac gtatcctggc ggcgcgccaa ccaaccggct gacgttgtga
     3481 cgctccataa attcagacat gtcaatctga atcagcgcgt cttcactgcc aaagaggaac
     3541 aatgccaggg cgcgggtgag cgcggtcttg cccacgcctg tgggacccag gaacataaaa
     3601 gatccaatgg ggcggcgcgg gtctttaagc ccggcgcgcc cgcggcgtac tgccttggag
     3661 atggttttaa tggcttcttc ctgaccgatg atggttttct gtaattcttc ttccatgcgc
     3721 agcaggcgtt cagattcttc ggtggccagt tgcaccaccg gcacgcctgt ccacatcgac
     3781 accagttcag cgatatcttc cgcgttcacc acggggctgg tagtgcggtc ccagccggtg
     3841 cgcaattttt caatcctgcc cctgaggtca gcctccttgt tagaaagttc catcgcgtca
     3901 tcactgcgtc cgctctgcac cgcctcgctg tgatgcgctt tcacatcttt gagttccagc
     3961 atcaactctt tgaaggtttg cgccgccgga ctcttgtaca tacgcacacg cgatgaagcc
     4021 tcgtcaatca ggtcaatggc tttatccggc aggaatcgtt cagtgacata acgcgaagag
     4081 agatgcgccg ccgcctcaag cgcttcgtcc gagatgacca ggcggtgatg ctcttcgtaa
     4141 gctttccgaa ttccttttag gatctggatg gtttcttcca cggtgggttc aaccaccaga
     4201 atgggctgaa aacgccgttc gagcgcagcg tccgtttcaa tgtgtttacg gtattcatcc
     4261 agcgtggtgg cgcccaccac ctgcagctct ccgcgtgata gcgccggttt gagaatgttg
     4321 gcagcatcca ccgcggaccc cgcagagccc gcgccaacca gcatgtgcag ttcatcgata
     4381 aacaggatcg atcgggaagc cttaagttca tcgatgacgc gcttgaggcg ttcttcaaac
     4441 tgaccacgat acatggtgcc agccaccagc gaacccacat ccagctgcag caccctttta
     4501 ccgatgagcg gtgcaggcac atccccttcg atgatgcgct gcgccaggcc ctccacaatg
     4561 gcggtcttgc ccactccggg ttcaccaata agagcggggt tgttcttgtt acgccttgcc
     4621 agaatctgga tgacgcgttc aatctcttgt tggcgcccga taaccgggtc tagcttgcct
     4681 tcttctgcca gcgttgtcag gtccaccgcc aactggtcca ccagcggcgt tttggcttct
     4741 tttttctctt cacgccgcgc caacggcgct cctccagctg aggccggccc actggcgctt
     4801 tcttccatca cgcgcctggt ctgccggcgc acctgttccg ctgatacgcc cagtttttta
     4861 agcacttcca gcgccagacc atccgtcaca cgtgtgagcc cgagcagcaa atgctcagtg
     4921 ccgatatact gatgtcctaa cttccgcgct tcctcaattg aaaattccag cgcctgctgc
     4981 gctccgaccg aaagctcgag cgctgaagac tcttgcagcc caatgggagc aaggcgctcg
     5041 accatttcct gcacgcgcgc ggtctcgagc cccagctcgc gtagcgcgtg cccggcaata
     5101 ccgccttcct cttcgataag gcccaataat atgtgttccg tgttgatcgc ggtgtggcgc
     5161 atgcgctctg cttcctggtg ggccagggag agcacccgcc tcgcgcgttg tgtgaaccgc
     5221 tccatacctg ccaaaacaaa tcctcctgat aactctcagc cattaatcaa ctggaaaact
     5281 gatcaatacg gcggatgtta ttcgccctca ttgtaccgtt attccttgtc attgtaatca
     5341 acaaatataa atatctgata agaattttgg ttttttacgc acaggtattt atatgctgcg
     5401 gaaagaggtt acagcacagg cagaaatgag cgagtgtaag tatttaccgg tcagacctgg
     5461 tcgggaatcg atctttatgg ctgtttcacc cgttcaatct aacaggtatg ccagtatcca
     5521 gttggctgtc gccgtcagcg catctgtgtg ggtacgctca taattatgtg aggcatccac
     5581 tcccgggcca atcagggcaa ccgccgcctc tcctcccgcg cgccagaagg cgctgccatc
     5641 agaaccataa taggggtaaa tatccacttt gtagggaata ccgtgattat ctgccagttt
     5701 gcgcaggtgg ttgctcaacc cgtgatgata aggaccgctt gaatctttta cacacagggt
     5761 cgcgtgaaac tcatctgaat tctgaccatc gccaacagcc gccatatcca ccaccaccag
     5821 ttcacctgct tcagccggaa gcccgtttgc cgcgccgtga cccacctcct caaaattgct
     5881 gatgaagaaa tatgtggttt gcgcaggctt taacccctcc gaacgcagcg ctcgcagggc
     5941 ttccaccagg caggcgaccg cagccttgtc atccagatga cgcgagcgca caaacccatt
     6001 ggtcagttcc acacgtgggt caaaggcgat gtagtcgccc acgtcaatcc ccagtgactt
     6061 cacttcctca acagaagccg ctttctcatc caggcgcact tccatggtat cgtcgtcgcg
     6121 cttcagttca gcggttttgg cgccatacac atggctcgaa gccgcggtca ccagcaccga
     6181 accacgtacg cgtctgccgc tgcgggtgaa gactgtgcag ccttcacctt ccactgtatt
     6241 ccaggcaaag ccaccaattt tactgagcga aagcctgccg ctgggcttga tgttttttac
     6301 catcgcgccc aacgtatcca catgggctgc cagggcccgc ggcgcgtttt gtctctggcc
     6361 ttctaacgtg gctaccagcg cgcctttacg tgtgcggctg gtttttactc ctggaatggc
     6421 tttgaacgcc cgttcaacga gcgccgtagc ctcttcagtg taaccggttg ggctgggtgt
     6481 cttcagcagg tcaaccagga acttaagcag taatgcttcg tcaatcaggg gtaaatcgtt
     6541 ttctgctttc tttataattc cctgccttct ctctctgaga acttgcgccg cagttcatcg
     6601 cttaacggca cggtcttttt cgtctcataa tcatagcaga ccataatgga ctcggccgtg
     6661 gcgtgcagcg gcgttccgcc ttcgccggtg atttgatact caaaggtcac ggtcttggcg
     6721 ctgatgcgag tggcgcccat ggagaccacc actttgtggc ccagttcaat gggctgccga
     6781 tagcgaatgt gaatgtcgcc aacgataatc ggcaattgcc ataacgcctt cccatccaga
     6841 agacccattt cctgtaagta gcggctgcgc gcgtcctcca ggtagaccag aaaacgcgcg
     6901 ttattgacat gccagaaggc atccaggtca ccaaacctga catcaattgg gtaatggtat
     6961 ttgaatggat tcatgagaat attatacttg aggttgttta cccaaagctc gagcgccggg
     7021 aggtcagcgc gcctcgatga tggttggcat cttttccttc gtcgattatg tagtaaaata
     7081 ttgaaagagg acaattttga ccctttttac ttatagaggt gtttttttat gaaaattacc
     7141 cggcaagccg attatgcgct cagagcgatg gtatacctct cgcagaaatc cgattctccg
     7201 acatccacca aagaaatcgc gaaagcgcag ggcatccctc cttcattttt ggcaaaaatt
     7261 atctcccaac tttcgctcaa gaagttaatc atcgcatcgc gcggcgcaaa tggtggcgtt
     7321 gcccttgcgc gcccggcttc caacatttcg cttcttgaag tgatcgaagc cattgatgga
     7381 gagatcgcgc ttaaccaatg cactgtttca attggtaatt gcgatagagc cgaagattgc
     7441 cccctgcaca tcatctggca ggaaacccaa gatgaactgg tgacaaaact acgcaataca
     7501 acctttgatc gcttcagtaa aaaaaactaa cagcagtcta acggtaatct gcgaaaggaa
     7561 ggggcaggtg gcgacaggaa caccgatcaa tcgacagatg gtcatcgtac tgaagaacgg
     7621 catcattgct attgattggg gtgatggtct ctaccaggac atccgtactg gtgacttcat
     7681 ccccgtgctt gaaaccgatt acagccatca tatacttaac gaagagctgg attggctcat
     7741 caagattggt agagtcattt catacgataa aaatactgtg caatctcaaa gcctgccgga
     7801 aaggccgcag cgaaccatag attaagctgc tttgttgcgc ttttttctcc cggccagacc
     7861 gggagttttt atttttggtg gataattaga ccatgttaac gcataaaacc ctgttacagc
     7921 gcattgcctt ctttactggt ctgcgcctca cactgaacac atctatccgc atgatttacc
     7981 cttacctggc ggtgtttgcg gttgccctgc aaagggatat tggcgccatc tctcttgtac
     8041 tggcggcttc catgttcacc agcgcggtgg gcccttttat agcccccatc gccgaccggc
     8101 gcggtcgaaa agtgggcatg ctcatcggca tgggcatctt ccttgcaggt acgttctcag
     8161 ccagcctctt tccctcctac ttcaccttct ttctggccat catggtaggc aatttaggca
     8221 acaatatctt cctgccttct atgcaggctt acctgggcga tcttgtaccc tacaacaaac
     8281 gcggtctgta cctcgccatt actgaactct cctgggcgct gtcttttatc ctgctggtgc
     8341 cgctcgcggg gttgatcatc acgaacacct actggtatgg acccttcatt gcgctcagcg
     8401 tgctggggtc ggtgatgacc atcctcttat gggtactcat cccggctgac catcccgcca
     8461 atcccgaacc cctggccatt ttcagtgata tcaaaaaagt gatcctttat ccacctgccc
     8521 tgttaagcat cctgatggga accctctttg tcgccggcaa cgagctggtg aatgttgtct
     8581 tcggggtttg gataaaagac tcattcggtc tgcagatagc cgctttgggc gccgcctcga
     8641 tcgtcattgg tctttcagaa ttgggcggcg agggagtatc cgccttgctg acagatcgcc
     8701 tgggtaaaga gcgcacaatc gccttaagcc tgatattgaa ttcgctatgg gtcattacac
     8761 tgccctggct ggggaaaacg accagcggcg ccatggtatg gttattcttc ttctacctca
     8821 cgtttgaagt ggcgattgtg agcacgctgc cgctgatgac cgaagtgacc cctgcgaccc
     8881 gcgccacgct cttgtctctc ttcgttgccg cgatctccct gggcaggtct ctggccgatg
     8941 tcttggcgcc cctgcttttc cgcggcggtt ttttggtcaa cgtgttggtg tgcgtggggt
     9001 tcaacctgct ctccattctg gcgctttcac gcattcgact tcctcgaaac gcaacctgat
     9061 gagcgttcaa gacgaccaaa ctccgccaga accgacccaa aaacccatct ccatgggcgg
     9121 gcaggtgttt cttttcgccc tggtgcgcat gatcatcaat atcaatacgc gcatggtnnn
     9181 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     9241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnctc acttttttgg gcatgttcgt
     9301 ttacctctct tcgactcagg cttatatcag cgaccatgtg cgggcgggat ggcgcggcac
     9361 cgcgctcggc ctggttgaga ccggctgggg ggtaagcttc atcctcggca tgcctatcct
     9421 gggctggctc attgaccgtt atggctggaa ggctcccttc cctttcacag cggtgttggg
     9481 cggcatcgcc ttcctgttgg tgtggatatt ggtacccagc cacaaacccg cgcaggcgcc
     9541 ttccaggagc gtgttgcaaa gcatatggga ggtgctgaag gtcccggcag cgcgctgggg
     9601 gttgctgatg agcctgatgc tcatctcttc caacgaggtc attaacctgg tattcggtgt
     9661 gtggctggag gtctcttttg gattaaagct ggcggcctta ggcgctgctt cagcggtcat
     9721 cggcatttcc gaaatcagcg gggaatcgat tggcggcgtc ttttcagata ggctggggcg
     9781 cgcgcgtagt attatgttgg gcattgggct gatgctggtg gtagcggctg cgctgccatt
     9841 catcggcggc agccaggctg gcgcgctcat cgggctgttc ttcttttatc tttcgtacga
     9901 attcactttt gtttccaccc ttcctttcat gaccgagctg gtgccgcagt tccgcggcac
     9961 cctgctgggc gccaacgttg cctccctctc cctgggacgc atgatcggca acctggtggc
    10021 gccgttcgtg ttcaccatcg gtttttgggc aaatgcactc gccgcggtct ttttcatcct
    10081 ggtcgctttc tgggcattgc tgcaggcaaa aaagccaaga ccagcagaat gacccccttt
    10141 tgacctcgcg gaaacaggta tactaacaga cgggagaaaa ttcatgctac ttcattccgc
    10201 ctcacaattg ctcacgcttg cgggcggtcc gcgccgcgga cctaacccgg tagacctggg
    10261 gctgatcctc gatggcgcag tgctggtgcg tgatggtctt atcgctgacg tgggctccac
    10321 gcaagaatta ctggcacgct accctaacga gcaacgcctg gacgcccgcg gaaaagccgt
    10381 gcttccaggc ttcatcgatc cgcataccca cctggtatgg gcaggcgacc gcgccgctga
    10441 gttcgaaatg cgactgcagg gcaaaacgta tatagaaatc atggcagccg gcggaggaat
    10501 caacgccacc gtacgggcca cccgcggggc aaccgctgag gagctttacc gccaaacccg
    10561 cgaacgcgct gacgccatgt tccgcaacgg caccaccacc gctgaagcca agaccggcta
    10621 tgggctggag ctgcaggctg agttggcgca gctggaaact ttggtgcgcc tggacgagga
    10681 agggccgctt gaaattgtcc ccaccttcat gccagcgcac gcggtgcctc ctgaattcaa
    10741 agggcgtgaa gatgattaca cccgcctggt ctgcgagcgc atgctgccaa tgtctgccgc
    10801 ctggtggaaa aagcgctgtc cgggcaagcg gctgcccttt gtggatgtgt tttgtgaaac
    10861 cggcgcgttt aacctggcgc agactcgccg cattctcacc agtgcccgcg acctgggttt
    10921 cccgttcagg gcgcatgtgg atgaatttga gaacctcggc ggcgccgccc tggcggtgga
    10981 actcggcgcg gtctccatcg aacacgcggt caaaacctca gcgatcgaaa tacaggcact
    11041 gggcaaatca gagacagtgg ttgtctcgct gccctgtaca ccctttgggc tggctgaacc
    11101 ccactacact ccagcgcgcc agatgctcga cgccggcgca atactggcgc tcgccacaga
    11161 cctcaacccc ggcaccgcct ggtgtgaaaa catgcagttc gtcatcgccc tggcctgccg
    11221 ctacctcaaa ctcacccctg ctgaagcgct ggctgctgcc accatcaatg ccgctcgcgc
    11281 catccgctgc gaggagcgtt taggctctgt agaggtgggc aagcaggcag acctgctcat
    11341 cctttctgtg gcggatttcc gccagctggc ttaccgtttt ggcggcagcc tggtggaggc
    11401 ggtgatcaag cgcggccacc tgaccagggt gaagtaatta attctagaaa taattaacgg
    11461 gctcggatgt tgactcttga aaaagcgcgt gaatggtata tccacactga ccctgtgcac
    11521 gatttttcgc acatcgagcg cgtgtaccgc atggcccagc gcctggccaa gttagaaggc
    11581 gccgacccgg agattgttaa cgccgcggct ttgctgcacg acgctgaagg caccacccct
    11641 ggcagcgacg cccgccgcga gcatcacctg cgctcggctg acttcgccgc caccatcctc
    11701 aaacaggaag gctggaatga ggaacgcatc agggccgtcc agcactgcat ccgcgcccat
    11761 cgttaccgcg acgaccgcga gcctcctcaa accatcgaag caaaggtgat tttcgacgcc
    11821 gacaaactgg atgtactggg ggctgtaggc gcggtgcgcg ccgtggtata cgccgcgctg
    11881 gcgggaaccc ctttttacgc cgccccatcc tcgcagtttc ttgaaactgg cgtggagctt
    11941 cccggcgaat tgcacagcgc ttaccatgag tacctcttta agttgcgcca cgtggagaaa
    12001 cgcctttata cgcaaaccgc cagggaaatc gcccgccagc gcagaaaata cctggataag
    12061 ttcttcatcc agctcatcgc cgaatacaaa ggagaaagat aatgacatca ccccaacaac
    12121 ctcagcttga acaggtcatc caatgggcca aagaggccgg cgacatcgcc cacgcaggcc
    12181 tctcgcaaaa gttcgatgtg gcgctcaaag gccgctcaga cctggtcacc gagatcgatt
    12241 accgttgtga acaacacctc atcaacgcca tccggtccgc ctacccggca cacgccattc
    12301 tttcagaaga ggcgggtgaa gttaacggag agactggcga ccgctggttc atcgacccgc
    12361 tggatggcac ggtcaattac gcccaccaca ttcccttcta cgcggtgtgc ctggcctggc
    12421 agagcggggc gcgcctgcaa ctgggcgtgg tctacgaccc tgcgcacgat gaatgctttt
    12481 acgctgaacg cggtcgcggc gcctggttga atggcgaacg gctcgcggtt aacaccactt
    12541 ctgacctcga gcgaagcctg cacgccaccg cctttgcccg ccaagacgaa cagctctttc
    12601 agcgcaacct gcagtatttc gcccatctgt cgcgccattc ctttggcgtc aggcgcatag
    12661 gctgcgcagc cctcgagctg tgctacgtgg cttgcgggcg tactgacgcc tactgggagc
    12721 agggtatcaa cgcctgggat attgccgcgg cggcccttat tgtggaagaa gccggcgccg
    12781 tggtaacagg gttggacgga gaccttgatt tcttcaaacc gccttacgcg cttctcgcgt
    12841 cgaccccctt gctgcacccg cagctggtaa aactctttgc tcaaatgcag taacaacccc
    12901 tcaagtataa ttacccctgc aggaaaacct gaatgaattc atcctgttga tcagaaggga
    12961 gtgaaatgac aaaaacatac acacaaaaag gctggagcct ggcttcgctt tacccctctc
    13021 atgacgggcc cgagatccaa tctgccttca aagaccttga aacacagacc gccgaatttg
    13081 aaaagaagcg cccgctgttg aaccccagta tcagccagaa ggagctgctt aaagtcatcc
    13141 aggcgcttga aagcctctat cgcatcgcct accgcctgag cggttacgcc gagctgtggt
    13201 tctctgctga tacacagaac cagagcgcgc tgggaatggt gatgcgtacc gaacagctgg
    13261 tttcggagat tgctaaccgc accctgttct tcagcctgtg gtggaaagac ctgcctgatg
    13321 aaaccgccag ccctttaatg gaaggaattg gcgactaccg ttactggctg gaagaaatgc
    13381 gccacttcaa gccgcatact ttaagcgaac ccgaggagaa gatcatcaac accaagaacg
    13441 tcaccggtgt gagcgccttc agcaccctgt acgacgctct caccaaccgc tacgtcttca
    13501 aagttgaagt ggatggcgag gtgaaggaat tgacccgtgg tgaattgatg agcctggtgc
    13561 gctcccctga ccctgacctg cgcgcccgcg cctaccagga actctaccgt gtctacgggg
    13621 acgacgcgcc catccttggg ttgatgtacc agaccatagt tcgcgattgg cgcaacgaag
    13681 aggtgacctt gcgcaagcac aagaccccga tttcagcgcg caacctcgcg aatgaccttc
    13741 cggacgaagt gattgagaca ctgctcgaaa cttgccgccg caacacaggc gtgtttgggc
    13801 gattcttcaa actcaaagcc aggctcctcg gcatggataa actgcgtcgc tatgatatct
    13861 acgcgccggt ggcgaaagcc gaaaaacctt acgcgtacga aaaagccgtt gccaaggttc
    13921 tggaatcttt cagccagttt gacccgcgct ttgcccaaat ggcagaacgc gtttttgctg
    13981 atgatcatct cgacagcgaa gtgcgtaaag gcaaacgcgg cggggcgttt tgttctacca
    14041 tcaacccggg aatcaccccc tgggtgctgc tcaactacca gggcaaggcg gatgatgttg
    14101 ccaccatggc gcacgagctg ggacacgcca tccattccat gctggccgag catcactctt
    14161 tcttcacgca gcacgctaac ctgccgctgg cggagaccgc ctccaccttc ggcgagatga
    14221 tgctggtgga ccagttgctg gccgaagaaa gcgacccgtc tgtgcgccgc gacctgctct
    14281 tcaggcaggt ggacgatgcg tacgccacca ttcagcgcca ggctttcttc gccatgttcg
    14341 aaaagcaggc tcaccaaatg gtggtcgatg gcgcgtcagt ggacgagatt tctgaagcct
    14401 acctggaaaa tcttaggacc cagttcggcg atgctgtcga tgtcgcagaa gagttcaaat
    14461 acgagtgggt ttccatcccg cacatctacc aggtgccttt ctacgtgtac gcctacgcct
    14521 tcggtcagct gctggttctg gcgctctaca agcgtttcaa agcagaggga gaaaccttca
    14581 aacccaggta tatccgcctg ctctcagccg gcggctcaga agcgcctatg aaactgctgg
    14641 atgaagcggg cgtagacgtg cgccaggccg ccttctggca gggcgggttt gatgtgctca
    14701 gcgagatggt ggataaactg gaaaaacttg cctgaggttc aaccatcaaa aaaggcagag
    14761 aagggctctc tgcctttttt gatggatact tcacagccac ccgatcgttg cgtcagcaca
    14821 gggggtgttt tttacgcgga gaaagccttc tttttctacc ccttcgcgaa ttagaaattg
    14881 cccgcgccag gattatggga cggtaaactc cacaaggcgc tcttttccgc ggttattctg
    14941 tctgcaccgg gatattctat ggtatatttt taattcaaca acatcccttc ccgtcgcgaa
    15001 gccgggagtg ttcacggagc cggtgtatgt attttgccat cgaaggagtc atcggggttg
    15061 gaaaaaccac cctcgccaga ctgctgcagc cgcagtttga agcagacctg gtgctcgagg
    15121 tcttcgaaga aaaccctttt ttgagcaatt tttataccga ccgcgcgcgc tacgcctttc
    15181 aaacccaaat cttctttctg ctcagccgct accatcagca gcgccgcaat atcggcgagc
    15241 ggctggggca ggcgaaaaac ctcatcagtg attacacctt tgagaaggat tcgctctttg
    15301 cgcgcattaa cctcacgggt gacgaattgg acatgtactt caaagtgcag gaagccctgg
    15361 cggagaagat catcccggct gacctggtgg tgtacctgcg cgccagcacc gacacgctga
    15421 tgaagcgtat tgccctgcgc gaccgcccct acgaaaggca aatggaacgg gaatacattg
    15481 acctgttgaa ccgcgtgtat gatgatttct acatctcctc tccccaggtt tctcatgtcc
    15541 ttacgatcga aaccgatgaa ctgaacatca ttgaaagacc tgatgacctg gctttcatcc
    15601 gcaaccgtat cagggaagaa ttgcggctgg cgccttacca gcccgttcta cccatcgagt
    15661 aaaagagggt tgaatgcgca tcacccgtga actcctgctc aaacacgccc gcgagaacag
    15721 cgccgcgctc acggcgaagg accgcagcat tatttgcatc tacatcagcg gctctttgct
    15781 gcaggaagac ccttttatcg gcggtatcac cgacattgat cttatctgcg ttcacaacca
    15841 gccggttgcc cggcgccgcg aagtgctgcg gttgagccac gaagttaacc tcgacctggc
    15901 gcattatgag caggaagaat ttgaacctgc gcgccaactc cgccacaacg cctggatcgg
    15961 tggctggttc gagaacgtgc ctatcgtgct ttatgatgcg ctgcgttggt atgacttcac
    16021 ccgcgcctcc gccaccgctc aattctggcg cccggaatat gttgcctcac gcgcgcggag
    16081 tttcttgaac cccgccagga agacctggaa tgacctcgag gagggcaaca ttccccaggg
    16141 catcaaacgg gtgaccgcct accttggagc gctgcgcgat accgccaacg cagtggctgt
    16201 tttctccggc gcgcccctgg cagagcgccg cctgctgatg gaactgcccg ccagggccgc
    16261 tgccgccggg ctcagcgggc tctttgctga ttttgtgagt cttttcacca gcagcgaagt
    16321 gagcgatgaa aacttcaccg catgggtgtc ttcttatgtg ggtattttcg aagccctcaa
    16381 acaatccacc accgctccag tgagcctttc cccctatcgg cgggggtatt atgaaaaggc
    16441 gctcaacgcg ctttatccgc acagccccgc agccgccgtc tggttgatgg tttatacctg
    16501 gaccaaagcg gctgccgcgc tgccacgcac cgaacagcca tataaagact ggcaggagct
    16561 ttgccgacag ctcgacctgg aaagccgcga cctgcccacc cgccttgaac tcttcgatgc
    16621 tgtgttggat accgtggaag aagcggttga ccatctttcc gcttaacctt tctcccgttc
    16681 tgtttgtaca caactcctct aaaacctgtg gataacttca aaaaaactgt ggataaatgc
    16741 atcgtttttc cacttttttt cgaaaaatcc gcccgaaacc ctgtggataa aaaggctgtt
    16801 ttctgccctt tatccacagc gtctcttttt tcagtcgctt tggcccaaaa aacggcttta
    16861 tccacatttc atgcacagtt acggctggaa agacggtcga ccacctaata gcatatacag
    16921 gggaaaacgt tacccctcaa tgcagggttg gcggataaaa gccttccggc tgtgtttctc
    16981 cacaaatcca cacccactac gacgactact acagattaat cttccatgag agataagtta
    17041 acaggatgcg caaacaaggc tgcgatgatg attgtatttg agagttgaat tgtgtacaat
    17101 aaaaatacaa aaaaaccaac cggctttggc tcgttgttcc ccggtttaat ccgggaggga
    17161 ggtcttatgg cagatatcat caaggtcaaa gcaaattctc gcactgcagc agtagccggc
    17221 gcaatagcag gtgttatgcg cgaacataag cacgtggata tccaggcgat tggcgccgga
    17281 gcggtcaacc aggcgctcaa ggcgctggtg ctggccaagg ggtatctggc agaagagggg
    17341 atcgaaatca tctgttcacc cgaatttgtg gatgtcgata ttgacggcaa ggtgcgcaca
    17401 gccatcaaga ttgtggtcga tccaaaaaac taagtctgcc aggcgggagt actaatttta
    17461 tctttcgtgc cctggtcaat ccggggtgtt agcttcttca cgggtaaaga tcacttcttc
    17521 tttgcccgtc agtttttctt tcaccttgct gatgatcaac tccaggtgtt gggtatgagc
    17581 cacataatcc agctcatccg cagggacagt gagcacaggg cagagcgtga agtgcgtgat
    17641 ccagttctcg tacaggctgt tgagatggtc aagataagct ggttcgatgc tgcgctcgta
    17701 atcccggttg cgtagcgcga tgcgcttgag cagcgtgggc accgacgcgc gcaggtagat
    17761 caccaggtca ggtgggggca ggtattcagc cagagtgcgg taaagcgagc ggtaggtggc
    17821 gtaatctcgt tcgctgatgt gcccctgcag aaagaggttc tgggcgaaaa cctcggcgtc
    17881 ttcatagatg ctgcggtcct gtattactga acccggcgtg cgtaccagtt cctggtgaat
    17941 gcgcaggcgg tgggtgagga aaaatatctg cgagtgaaag ctccacgcgg gcatatcctt
    18001 atagaaatcg gacaggtaag ggttctctgc taccggctcg tagaaaggca tgcattccag
    18061 ccgctcactg atcatttcca ccagggtgga cttgcctaca ccgatgttgc ccgcgaccgc
    18121 aatgaatttt ttcataacat cctcagcaag gtgaaaggtg tgttgagaga aagaaccctc
    18181 aatcctgcga accgtcaaga ttggaggtgg attttttcgc ttcagcagcc agagctctct
    18241 ttgaagaggc tttctaccgg gctgcggcag ccagtgagtt cataccctgt tttgattata
    18301 acattgcgcc ttgtctcaca atcgagggag gtcgtggatc ggagcaaccg cgaggtcaag
    18361 tgctctcatt ggttttaacc cagaaggcat tcaatcgttc acaaaatcct catcataaat
    18421 tcacattttc ttcataactg gcggctatca tcgagttaat caagcagaga gaagctctgc
    18481 aaaaaaggag ttgcaaatga aaaaaatatg ggtatttctt gtcattggcg gcgtgctggc
    18541 tttggcgcta ggagctgcct cggtggtgaa cgcgcaggca ccggtgaaca ccccccaggt
    18601 ggagcaggga ggaggacaag gcggttttgg cgcgcgtgct gctggttgcg gcgaacgcgg
    18661 tgaaaacgaa gaagtgcaga agttgatgtt gaacgcctgg tctgacgcgc tcggcatcag
    18721 cgtggaagag ttgagtaatc gcaaagaggc tggcgagaac ctggcgcaga tcgccctttc
    18781 caccgggcgt acttttgaag agttccgcgc gctcagggtt tcggtgcaca cttctgtggc
    18841 tgaacaggca ctcaaggacg gtatcatcga ccaggcgcag taccagtgcc atctcaaggc
    18901 ggttgagcgc cagatgcacg gtcgcggcaa cggcaaccgc cctgccgacg ggcacgggtt
    18961 caccacgcgc ggcagagggg atcgtgccag gggacatcat tgcgggggtc gctccgccaa
    19021 cccctaaacg ctgctccaca aaatcgcaaa ctgttcaggg ctgccaatac ggcagccctg
    19081 ctgttttaga gggtataatc cggcgtaatt cacccggttc taccatgcca caggataaga
    19141 cccttcttcc ttctctctcc aaaccgcttg ccaggctcgc gcccgccctg ccagtggtgc
    19201 tgggatacct gcccatcggt ttcgccttcg gcgtgctggc cgccgcggcc ggcatggggg
    19261 ctttcaacag cctgttgatg tctctcctcg ttttcgctgg ctcggcgcag ctggtggcgg
    19321 ttgaactggt ggcgcagggg ctcacgccct ggtcgattgt tgccaccacc ttcattatca
    19381 acctgcgtca cctgttgatg tcggcgtcgc tctccccgta catgcaggag tggcgcccgg
    19441 cgcaggtggc tggctttact tttgaactca ccgatgagac cttcggcgtg cacagtctgc
    19501 gctttgaacg cggagaaacc cgcgcgatac tggcgctgct catcaacctg gtttgccacc
    19561 tggcttgggt agccggtacg ctgttgggga tgctggctgg cgggctcatc cgcgatgtgc
    19621 gcccgctggg gctggattac gccctgccgg ccatgttcat cgccttattg gtgatgcaga
    19681 tacgcggcag ggtctacctg gtggtggcgc tgatggcggc ggggctttca ttgttagtgt
    19741 ggagcgccgg gttcacgcag tggaatgtga tcatcgcggc ggtgctggcc gccgccgccg
    19801 gagcaggagc agaacgatgg cagaaacaga cctcctgatc accgttctgg gcatggcgct
    19861 ggtcacttat ctgccgcgcc tgcttcccgc ctggctgctg cgcgggcgcg cgctgccgcc
    19921 tttcatcact gcctggctgc gttacattcc agccgcggtg ctggcggcgc tgctgctgcc
    19981 ttctctgttg gtggagggcg ggcgcctgaa cctgacctgg gataacctgt acctgtgggc
    20041 ggcgctgccc gctgccctgg cagcgtggaa atggaagagc ctgttcggca cagttctggt
    20101 gggcatggcg ctggtggcgc tggcgcgcct gctgggatgg tgagggaaaa cttcgcttga
    20161 caggtttggg gcatgaatta taataatggt cgccttcctc gttagctcaa tcggcagagc
    20221 gggcggctgt taaccgctag gttcgaggtt cgagtcctcg acgaggagcc ttagccttag
    20281 aaagacaccc aaaatcgccc agggtgtctt ttttattaca agaaacccat tgatactata
    20341 atttgttaat aagtagagat tggagattga tatgaaaatt aacacaagaa ttttttcata
    20401 cagatttgct gaacagtaca aaggtagaaa ataaatgagt tttctcaaga gactactaat
    20461 aaatttagcc ttcttaattg gtctcggaat tttgctttta gttttttttc cagacatgat
    20521 gaaacaggta tatcaaatgc taggtgagtt atttggtccg ttagtgattc ttatggttat
    20581 agttgctgcg cttccaagaa aacggagatc aagaaattaa gagaacatag agtcagttct
    20641 gtttttcttc atatcgtcaa gttatgaatc cacccattta ttatctttct gtattttgca
    20701 cccccgtact gtaatacggg ggatttccgg tttcccggtt tatcttcttc tattaccccg
    20761 ggcgcctcga cagccaagag agtcgtcacc tcgctcttga ccgtctcgtt atgcgcctgg
    20821 ggctggagta tcgcgtgaaa cctgaagggt ttcacgcggt gaataataat gtatatattc
    20881 aagcaatgct tgaaactcag aataagtaat ttgtatgatt atagaaaaaa agtaattttg
    20941 ctcattacaa ctagt
//
LOCUS       DAOTLW010000033        18899 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_3316_length_18899_cov_10.839312, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000033 DAOTLW010000000
VERSION     DAOTLW010000033.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18899)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18899)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18899
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3316_length_18899_cov_10.839312"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            111..2513
                     /locus_tag="P5516_06360"
     CDS             111..2513
                     /locus_tag="P5516_06360"
                     /inference="COORDINATES: protein motif:HMM:NF021492.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HRT92097.1"
                     /translation="MIKRALTSISILVFVLTSCTLPFNRTGENRGIPSGTNSAPQLSP
                     TPTITPTPQPQARITLGQADILNGDYEKALAEFWSAREQSTDPEVIQAAQLGVGRVLL
                     LQNDINGAVSQLNWLLTNFAEGESRDTAYFFLGQAYERLQQYLLAADAYQNYVDASPG
                     PLDSEILEMKGDALVRAGEFGAAKLAFLAALKTAQPIREDTLQLKIAQAAAESGGEQD
                     AISIYWALLENSSADYIKAQSNLLLGRLYLKLGMPEQAYARFQDSVTRFPTYYDTYSG
                     LVTLVEDGQPVNDLMRGIVDYYAGQYSLAVIALERYMLENPDHDGTPLYYKAYGYYRL
                     GDIDQEIAAWNELIEEHPFDQYLATAYLEKASSMWRYQQDYEGATNTLLHYAARYPDS
                     ARTPEFLFLAGKILEIGGYLTRAAETWQRVFNEYPGAEQAYPALFKAGIVYYRLQRYE
                     DARLVFQRMLVLSTSPEDQAAARFWIAKCLEAEDKDEQALTYYQQAADADPTGYYGIR
                     GGQKSRGEPPFPRQNNNDIAMDTDKEKKDAEKWLRATFSLDQGVDLHSYSDLSNNPLF
                     QRGEEYWKLGLRDNARNEFEQLRKELEGDAVNLYRLMNHMLDLGFYQTASLSSRQILD
                     LAGLSQDETLTKAPIFFNHIRFGVFYRDLVVPAAIEHGLDPLLIFSLIRQESLFDASI
                     TSSAGAHGLMQITGDTGETIVNNYGWPENYTVESLDRPFINVRLGTHFLKIWIDKYQG
                     EITPALSSYNAGDGNTIIWKGLAGDDVDLLVELIRFDETRDYIKYITENYEIYKTLYS
                     HP"
     gene            complement(2588..5368)
                     /gene="ppdK"
                     /locus_tag="P5516_06365"
     CDS             complement(2588..5368)
                     /gene="ppdK"
                     /locus_tag="P5516_06365"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HRT92098.1"
                     /translation="MTDKKWVYLFNQIKEVEQYVNKDWDAVRGLLGGKGANLAEMVRI
                     GLPVPPGFTVTTEACNAYQETKQFPEGMWEQMLDCLQLVEKSTKKKFGSTRNPLLVSC
                     RSGAKFSMPGMMDTVLNIGLNDETAKGMIKLTQNERFVYDSYRRLIQMFGSVVLGIPD
                     EAFEKHLDGLKQVKGYKLDTEMTASDLTGLIEVYKKVIFEEMGFEFPQDPLEQLKLAT
                     EAVFKSWDGKRAVDYRRATHIPDDLGTAVNIQTMVFGNMGEDSGTGVAFTRDPSTGEN
                     KLYGDYLLNAQGEDVVAGIRNTEKIAHLGRDLPEANKEFLQIAKKLELHYKDMQDVEF
                     TIEHGKLWMLQCRNGKRTALATVKIAIDLANEGIITREDAVMRVSPTDVDTLLHPQFS
                     SETKKLAVQEDRLFAHGVNASPGAAVGQVYFDADTAERRAKEYGESTIMVRPFTKPDD
                     VHGMLAAKGILTSEGGATSHAAVVARQFGVPCIVGAAAVKIDLDKREMNANGVLIKEG
                     DWISIDGSTGEAYIGKLNLTTPKFEEQIDLLNLLSWADQISSTKGIRHAPDGWPTTGL
                     QVWANADNPRDAERARTYGAVGIGLCRTEHMFFETERLPIVQRMILAKTSEERTAALD
                     ELLPYQRRDFEGLFEAMNGYPVIIRLIDPPLHEFLPSAEELQSKIIALRLSGGGLSNF
                     VLGRGELKETADLDTMLDAVNAMHESNPMMGMRGVRLSIAMPEIVEMQVRAIFEAAAN
                     IANKGMTPKPEVMIPLTGHVNELTWIQPRLERIAKAVMEEKKVKFDYKFGTMIEIPRA
                     AVTAGEIARVAEFFSFGTNDLTQMTFGYSRDDAERNFLTTYIEQEILPKNPFQTLDQE
                     GVGKLMQLAVQDGRLARVNLEVGICGEHGGDPASIDFCHRIGNNYVSCSPFRVPIARL
                     AAAQSAIKHRSK"
     gene            5581..5994
                     /locus_tag="P5516_06370"
     CDS             5581..5994
                     /locus_tag="P5516_06370"
                     /inference="COORDINATES: protein motif:HMM:NF012301.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT92099.1"
                     /translation="MKILIIDDDPSVTDLLRLILAPTLAEIQHACDGEQGSGLVREFK
                     PDIVLLDYMLPGIDGLETTRQIRKITKAPILILSVLDDPLTLAKALDAGADEYLIKPV
                     SRGVLIAQINNLVRRYSEKSVDHSPLKVSWQTALQ"
     gene            complement(6006..7328)
                     /locus_tag="P5516_06375"
     CDS             complement(6006..7328)
                     /locus_tag="P5516_06375"
                     /inference="COORDINATES: protein motif:HMM:NF025112.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92100.1"
                     /translation="MTEPSKPLYLVGGAVRDALLGKPCSDFDFVCAQDPRPMARAFAN
                     RNHGNYFILDEGRLICRVLIDSAEVPDLIVDFAIMRGENILEDLAHRDFTINAMAIDL
                     NQPEHIIDPFKGGRDLLEKRLRVVSRDSFIEDPLRVIRAVRYTVDLGLTLAPEIIDLV
                     RAAVNGLQRVSLERKRDELFKIFSGHKVHTALQLCMLFGIIPYLPLSIPLNFPPLVTR
                     IRSLEDILDWLCGVKTHARQTAFYQSSLLVELGRFREDLRRFYLAKNPSKRDRRSLLF
                     LSVILGEQAEPGDHVRALALSVEEMKQVRDFKAAIPLLQGFIDRGSIPEPLEIYRYFK
                     ATAAGGVDAAVAALAEQVSHVGCEFVQEQWLYQLKLAARLMEAWFKSPQVLNPDPLLN
                     GDDLMRRFSLSPGPFIGELLELLKEQQIKGSVTSASEAWRWIEGELKK"
     gene            complement(7351..7935)
                     /gene="hpt"
                     /locus_tag="P5516_06380"
     CDS             complement(7351..7935)
                     /gene="hpt"
                     /locus_tag="P5516_06380"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HRT92101.1"
                     /translation="MQDYHYYLSEVLITEDALQRRIAELGGQISRDYAGKGELVLVCI
                     LRGGVLFLTDLMRKVTIPHAIEFMAVSSYGVGGRESNGQVRITLDLNLDIFNKHVLLV
                     EDIIDSGHTISAVIDFLATRRPASISVCVLLDKEERREVEVPIRYRGFTIPNKFVFGY
                     GLDLDDYYRNLPFIGVVDLQKYQGSEQGSEGAIS"
     gene            complement(7952..8524)
                     /locus_tag="P5516_06385"
     CDS             complement(7952..8524)
                     /locus_tag="P5516_06385"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HRT92102.1"
                     /translation="MDRKSGHIEVICGSMFCGKTDELIRRLRRATIAKQKVQVFKPAI
                     DGRYGLQKVTSHAGSEFGATPIRSSSEIIPLLKPETTVVAVDEAQFFDDELIEVVEEM
                     VTRGLRVVIAGLDMNFKGEPFGCMPALMARAEQVDKLQAICMVCGEPASRTQRLVDGK
                     PARYDDPVVIIGASEMYEARCRKHHEVPKD"
     gene            complement(8643..8927)
                     /locus_tag="P5516_06390"
     CDS             complement(8643..8927)
                     /locus_tag="P5516_06390"
                     /inference="COORDINATES: protein motif:HMM:NF015102.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HRT92103.1"
                     /translation="MQARQDYVEEKKSKLAAKTSPERSVKDIGITVKAAEALEKAGLM
                     NVGQLLEKLAEGEATILAIEGFGRKSLIDMKKNLRQMGYKLPAAAEEIVI"
     gene            complement(8916..9119)
                     /gene="rpmE"
                     /locus_tag="P5516_06395"
                     /pseudo
     CDS             complement(8916..9119)
                     /gene="rpmE"
                     /locus_tag="P5516_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003151132.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            complement(9188..9451)
                     /gene="rpmA"
                     /locus_tag="P5516_06400"
     CDS             complement(9188..9451)
                     /gene="rpmA"
                     /locus_tag="P5516_06400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002513859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HRT92104.1"
                     /translation="MAHKKGGGSSRNGRDSNSQRLGVKKFAGEQVLSGNILVRQRGTR
                     IKPGLNVKVGTDDTLFAVAHGIVEYETLPSGQKQVHVRPVETK"
     gene            complement(9457..9774)
                     /gene="rplU"
                     /locus_tag="P5516_06405"
     CDS             complement(9457..9774)
                     /gene="rplU"
                     /locus_tag="P5516_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HRT92105.1"
                     /translation="MKYAIVESGGKQYRAVEGTTIDVDLLDVEVGHEVKLAEVLLVVE
                     DEKVQVGTPSVKGAVVTTSVVDHIKGPKVDTFKYSPKKRIRVKSGHRQRYTRLKIESI
                     NLE"
     gene            10208..13240
                     /locus_tag="P5516_06410"
     CDS             10208..13240
                     /locus_tag="P5516_06410"
                     /inference="COORDINATES: protein motif:HMM:NF024589.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT92106.1"
                     /translation="MALLLSYLALHADAALTRRQVAYLFWPETGDEQARTNLRNLIHY
                     FKKALPEADALLELDGQTIRWRDTSSLSVDALQFRAALAATRAVQDDETRIRHLQEAV
                     NRYRGELLPGHYQDWVLLIREEFQQAFLDALAQLTKRLEEVRRYEEAVEAANRLIRAD
                     ALNESAYLQSMRLRALMDDRAGALQVYYACATTIRRELGVEPSQEIQSFYQRLVGAEQ
                     NAQAQFREEKAPEQMRLVGRKQEWSRLKEAWGSVQKGKAALVLILGEAGIGKTRLASE
                     LMQWARRQGIRAARAECFAAEGNLPFAPIVAWLRTPEIREERAGLEALWKHELARLLP
                     EDAAGGTPSSGRKWERRRLFEAVARGLLGSGAPRLLMLDDAQWSDSETLEIVHYLMRY
                     DANAALMILMTARVEELDEEHPLTQLRLSLQERKQLHELELMPLDKPELGLLARDVLG
                     RDLEKAEEDELFRQTEGNPLFIVETLRSGKINETGMPQTLRSVLTRRLGGLSAAAREL
                     VGVAGVIGREFRYPLLARASQMNESVLVQALDELWLRRVIQIQRADAYNFTHGKLQEV
                     AYEALSAPRQQLLHRKIAESLEVEREDPAVIARHWDAGGQSAQAVEAYRRAAEAARRV
                     FANSDALAHLERALELLKEKIGAEMEEQKKSAGEICEALGDIYEMGNNQKSLERYREA
                     LTLVESSDGLSLARVLGKLARAEANHGNLQEGLNLFAQAEAALGEPSEAEDWQRAWLK
                     VHFDRVWVEYDANRVEALEAALAPIRPVVERLGELEKLGEYYFLFPTAYFRRDGYQIN
                     DEIMRYSKLALETSAKTDNLELQTRTNFGYGFCHFLRGDFDEAIHYLGEGLRLAEQIG
                     YVELQLMSVTYLAAAYRGAGRVEECQTCAERALTLSEREGFLAYVAAARANLGWAAWR
                     RSNLRQAKALSLSALEMWGAGYPFRWLALWTLIDLSLHSLRTDEALEYARKLKEPHQQ
                     IFEKEGDDLLIQASSADVGNAAPLLAQALAWAKKRGYL"
     gene            13465..13827
                     /locus_tag="P5516_06415"
     CDS             13465..13827
                     /locus_tag="P5516_06415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92107.1"
                     /translation="MKALRITLLVFTIYTAIIGILFLFAPRIAETAFQTRLPDAALTM
                     LYGQVVLVLALAAWLIRSDAAALRKMVWALVFAEVGHVVVFLWQLMNGISTFAQVGPP
                     MIIAAVFAVLFVVFNRKG"
     gene            13833..14042
                     /locus_tag="P5516_06420"
     CDS             13833..14042
                     /locus_tag="P5516_06420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92108.1"
                     /translation="MRVEGNPAGARTHLFTLRIWAVNEGTAAPKWRSRLQNIRSGEVF
                     FCENLSDLLSHIEETLCEQDQDEVP"
     gene            complement(14467..16350)
                     /gene="selB"
                     /locus_tag="P5516_06425"
     CDS             complement(14467..16350)
                     /gene="selB"
                     /locus_tag="P5516_06425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HRT92109.1"
                     /translation="MRVIGTAGHVDHGKSTLVKALTGIDPDRLKEEKKRQMTIELGFA
                     WFESQSEGEIGIVDVPGHRDFIDTMLAGVGGIDAVLLVIAADEGIMPQTKEHLAIIEL
                     LRVNTGLVVLTKVDLVDDPAWLELVEMDIRERLSSTCLENAPLLRVSAVTGDGIAQLR
                     AAIEEVLAPLPPRLDLGRPRLPIDRIFSLTGFGTVVTGTLLNGSLCVDNEVEILPSRR
                     SARIRGLQTHKRSEETALPGSRTAVNLSGVDVADIKRGEVITLPRLLEPTQRIDAQIE
                     LLQDAPVSLKHNDLVKVFALTSECAGRVRVLQGQELKPGENGWVQIEFEKEMVLEKED
                     RFIIRRMSPAHTLGGGVVVNPHPGRRYRLQDSSVIERLSLNSGTALENRILALLMEKP
                     FITVDEIQNRLETDSERVREILAELTGRGQVSVLAGLTDQTTGYLPRAKWAELTAKSV
                     QLLQHYHQQNPLKSGINVDELARRLRISQDELNLCLREWLKEKVVKLQDGAIALPGFE
                     VRYSSNQSRRLEQFTAILDQQPFLPPGVKESKDLLTDDVFQSLVERGALVQLSTEVVV
                     RKAEYGLMLGYVIERCSRGELLTLAQFRDHFNTNRKVSQAFLEFLDRKGVTQRVGEGR
                     VLK"
     gene            complement(16354..17532)
                     /locus_tag="P5516_06430"
     CDS             complement(16354..17532)
                     /locus_tag="P5516_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92110.1"
                     /translation="MENEEIVKRIEWLDGERRKDRQTIIELQAQVSKLQEVIALQKRE
                     LSNINKDNKKSFSSVVRLDEYEETKAKLKAELAKSQRLIEKRLDTMEQTTAAQRSDDR
                     DTYNKRMLELQGEIKALNELKKSVQSKGEDDFRLNQKLEDLARQLADLRSSGDEQNRL
                     AKILDDNYRVESKRLSDLQIELNTLRKRIDEDRNLSDAQKEYLKKLEGQIHDLVNREK
                     TRNQEQIEFIEKQSLLSVDRENLWKDWQEEIQQMKALSENFHGQVRELETLNREIKQG
                     QSDFEVINQRLERRINEITEMNRLTEERFRQEWIAFKADDQKRWTNYSLSQEETNREG
                     NREINKIDERITSLEDAVQNLLDTVQVIHEETEKRIKGLLMLANEYITSYERTALKKG
                     "
     gene            complement(17562..>18899)
                     /locus_tag="P5516_06435"
     CDS             complement(17562..>18899)
                     /locus_tag="P5516_06435"
                     /inference="COORDINATES: protein motif:HMM:NF012581.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proton-conducting transporter membrane subunit"
                     /protein_id="HRT92111.1"
                     /translation="FLLKYPTILKLTGIVVSLLLALIAFFQPIGNVLRLGGMVLDIRP
                     DLQFLGRALHLDNGDRFILAGLFGILFIYTFLWNTHTVPAKFAPLSLAISAILVAALA
                     VQPFLYAAVLVELAVLLMIVLVKDNQMNQEKGVIRFLVYLTLAMPFILFSGWILGGTQ
                     ASPSEQGNLLGAAITLMIGFSLWLAVFPFHSWVPQFSRAVQPYLSGFIISLFPIVVLF
                     VAVNYISSLVWLRSASYLAPVLSSTGIIMIVTTGLWASVERDVKRVLGYCVLLETGFS
                     LIMISFKSEQGLILMYHSLFPRVLSLALMVYSLSILHQAGIALTHDGFQGVIRQYPFA
                     ASGFLISLFTISGFPLFAAFPIRLELLYQLGHISVPSVIWTVFGLSGLIFAVIRLFRV
                     MTLPAPNNWRIHESVTQVIIICTVILVLILAGLFPQLVEGIIKPFTADLPLLK"
BASE COUNT         4476 a         5130 c         4800 g         4493 t
ORIGIN      
        1 atattttgcc gcgggattgg aacctgatta aaccaaatca cgtcgaaaag tcgtatacaa
       61 tacggtatcc gctggttgac gcccaatcaa acctccggta taagaggatt atgataaaac
      121 gagccctgac ctccataagc atcctggtgt tcgtgttgac ctcctgtacg ctgcctttca
      181 atcgcacagg cgaaaatcgc ggcatccctt ctggcacaaa ctccgccccg caactttcgc
      241 cgaccccgac catcacaccc acccctcagc cccaagcgcg catcaccctt gggcaggcgg
      301 acatattaaa tggcgattat gagaaagcct tagccgaatt ttggagcgcc cgtgagcaat
      361 ccaccgaccc ggaagtgatt caggcggcgc agctgggtgt gggcagggtg ttgcttttac
      421 aaaacgatat caacggcgcg gtcagccagc tcaattggct gctcacgaat ttcgccgagg
      481 gagagtcgcg cgatacagct tacttcttcc tggggcaggc ttacgaaaga ctacaacagt
      541 acctcctcgc ggcagacgcg taccagaact atgtggatgc cagcccaggt ccattggata
      601 gcgagatcct cgaaatgaag ggagacgcgc tggtgcgagc cggcgaattt ggcgctgcca
      661 aactggcttt tctcgccgcg ttgaaaaccg cgcagcccat tcgcgaagat accctgcaac
      721 tgaaaattgc tcaggcggct gctgaatctg gcggagagca ggatgccatc agcatctact
      781 gggcgctgct ggaaaacagt tctgcggact atatcaaagc ccagtctaac cttttactcg
      841 ggcgccttta cctgaagctg ggaatgccgg aacaagccta cgcgcggttc caggattctg
      901 ttacccgctt tccaacctac tatgatactt attccgggct ggtaacgttg gtggaagatg
      961 gacaaccggt caacgacctc atgcgcggca ttgttgatta ctacgcgggg caatacagcc
     1021 ttgccgtcat cgcgcttgaa agatacatgc tggaaaaccc cgatcacgat ggcaccccgc
     1081 tttactacaa ggcttacggt tattatcgcc tgggagatat tgaccaggaa attgccgcct
     1141 ggaatgaatt gattgaagag cacccattcg accaatacct ggctaccgcc taccttgaaa
     1201 aggcttcctc gatgtggcgt tatcagcagg attacgaagg cgccaccaat accctgctgc
     1261 attatgccgc acgttacccc gattctgcgc ggacgccaga attcctcttt ctcgcaggca
     1321 aaatattgga aattggcggc tacctgacca gggcagctga aacctggcag cgggtattca
     1381 atgaataccc tggagctgaa caggcatacc ctgctctgtt caaagccggt atcgtctact
     1441 accgcttaca gcgctatgaa gatgcgcgcc tggtctttca gcgtatgctg gtgctgtcaa
     1501 cctcccctga agatcaggct gcggcgaggt tctggatcgc caaatgcctt gaagccgagg
     1561 acaaagacga acaggcgctc acctattacc agcaggcagc ggacgctgac cccaccgggt
     1621 actatggtat tcgcggtggg caaaagagtc ggggcgaacc ccccttccct cgccagaaca
     1681 acaatgacat cgcgatggat accgataaag aaaagaaaga cgcagaaaaa tggctgcggg
     1741 caactttttc gcttgatcaa ggggttgacc tgcattcgta cagcgaccta tcgaacaatc
     1801 cgctgttcca gcgcggcgaa gagtactgga agctcggctt gcgcgataac gccaggaacg
     1861 aatttgagca actgcgaaag gaactggaag gggacgcggt caacttatac cgtttgatga
     1921 accacatgct tgaccttggc ttttatcaaa ccgcctcgct ctccagccgg cagatacttg
     1981 atctggcggg cttgagccaa gacgaaaccc tcaccaaggc accgatcttt ttcaaccaca
     2041 tccgctttgg ggtattttat cgggacctcg ttgtaccggc ggcgattgaa cacgggctgg
     2101 acccgttgtt gattttcagc cttatccgcc aggaaagcct cttcgacgcc agcatcactt
     2161 cgagcgctgg agcccacggg ctgatgcaaa tcaccggcga taccggcgaa acgattgtga
     2221 acaactatgg ttggccagaa aattacaccg tcgaatctct cgataggcca ttcattaacg
     2281 taaggttggg tacccatttt ctgaaaatat ggatcgacaa gtatcagggt gagattaccc
     2341 cggcgctctc gtcgtataac gctggggatg gaaacacaat tatctggaag ggtttggctg
     2401 gagatgatgt tgacttgctg gtggaattga tccgctttga cgaaaccagg gattatatca
     2461 agtacatcac cgagaactac gagatttaca agacactcta ttcccaccca tgaacaggca
     2521 ggaagatgga taagaaaaac ctccctggct gtcagcaggg aggttttgta tcaacccgtt
     2581 tttacggtta cttgctgcga tgtttaatcg cagattgggc ggccgccagg cgcgcgatgg
     2641 gcaccctgaa gggtgagcac gacacatagt tatttccgat gcgatgacag aaatcaatgg
     2701 aagcagggtc tccgccatgc tcgccgcaaa tacccacctc gaggttcact ctcgctaaac
     2761 ggccgtcctg cacggcgagc tgcattaact tgcccacgcc ttcctggtca agcgtttgga
     2821 aggggttctt ggggagaatc tcctgttcga tataagtggt gaggaagttg cgttccgcgt
     2881 catcacggct gtaaccaaaa gtcatttggg tgaggtcgtt ggtgccaaac gagaagaact
     2941 cagccactct ggcgatctcg ccggctgtca ccgcggctcg cgggatctcg atcattgtgc
     3001 cgaatttata gtcgaactta acttttttct cctccatcac cgctttggcg atacgttcca
     3061 ggcggggttg tatccaggtc aattcattga cgtgacctgt gagcggtatc atgacctcag
     3121 gtttgggtgt catcccttta ttggcgatat ttgccgcggc ctcgaagatg gcgcgcacct
     3181 gcatttctac aatttctggc atggcaatgc tcaggcgcac cccgcgcata cccatcatgg
     3241 ggttgctttc atgcatggca ttgacggcat cgagcatcgt gtcaaggtca gcggtttctt
     3301 ttagctcacc gcggccaagc acaaagttcg aaagccctcc accgctcaag cgtaaagcga
     3361 taatcttgga ttgcagttct tcagccgaag gcagaaattc atgaagcggc gggtcaatca
     3421 ggcgaatgat gaccgggtaa ccattcatgg cttcaaacag gccttcgaaa tctctgcgct
     3481 ggtagggaag cagctcatcc agtgcggcgg tgcgttcttc gctggttttc gccaggatca
     3541 tccgctggac gatcggtaac cgttccgttt caaagaacat atgctcagtt cgacagagac
     3601 cgatccccac agcgccgtat gtgcgcgccc gttccgcatc cctgggatta tccgcattgg
     3661 cccacacctg caaaccagta gttggccagc catctggcgc atggcgaata cccttggtgg
     3721 aggagatctg gtcggcccaa ctgagcagat taagcaaatc aatttgctct tcaaattttg
     3781 gcgtggtgag gtttaactta ccgatatagg cttcaccagt ggaaccatca atcgaaatcc
     3841 aatcaccttc tttaatcaaa acgccattcg cgttcatttc acgtttatcc aaatcgatct
     3901 tcaccgcagc cgcgccaaca atacacggta cgccaaattg cctggcaacc accgctgcgt
     3961 gcgaggtcgc cccgccttcg ctggttaaaa tacccttggc cgccaacatt ccatgtacat
     4021 catccggttt ggtgaagggg cgcaccatga tcgtggattc gccgtactct ttggcgcgcc
     4081 gttcagcagt gtctgcgtca aaatacacct gacccactgc cgcgccagga gaagcgttca
     4141 cgccatgagc aaacaggcgg tcttcttgaa cagccagctt cttcgtctcg ctggaaaatt
     4201 gcgggtgcaa gagggtatcc acatctgtgg ggcttacccg catcacggcg tcttcacggg
     4261 tgatgatgcc ttcattcgcc aggtcgatgg cgatcttcac cgtcgccagc gcagtacgtt
     4321 tgccattacg gcactgcagc atccacagct tcccatgctc aatggtaaat tcaacatcct
     4381 gcatgtcctt gtaatgcaac tccagctttt tggcaatctg caagaattcc ttgttggctt
     4441 cgggcaggtc tctgccgaga tgggcgatct tttccgtgtt gcggatgccg gcaacgacat
     4501 cctcaccttg cgcgttcagc aggtaatcgc cgtacagttt gttctcgcca gtggatggat
     4561 cgcgggtgaa tgccacaccg gtgccggagt cttcacccat attgccaaac accatggttt
     4621 gaatgttaac ggcggtcccc aaatcatctg ggatatgggt ggcgcggcga taatccaccg
     4681 ctcttttccc atcccacgac ttgaacaccg cttccgtagc cagttttaat tgctctaaag
     4741 gatcctgggg gaactcgaag cccatttctt caaaaatgac ctttttatag acctcaatca
     4801 atccagtcag gtctgatgcg gtcatctcgg tatccaattt atagcctttg acctgtttca
     4861 aaccatccag atgcttctcg aatgcctcgt ctggaatccc cagtactaca gacccaaaca
     4921 tctggatcag gcggcggtac gaatcatata caaagcgctc gttctgcgtg agtttgatca
     4981 tccccttggc ggtttcatca tttaaaccga tgtttagaac cgtgtccatc atccccggca
     5041 tggagaattt tgcgccagaa cggcaagaga ccagcagcgg gtttctggtg gagccaaact
     5101 tctttttagt tgatttttcc actaattgca ggcagtcaag catttgctcc cacatcccct
     5161 ctgggaactg cttggtttcc tggtacgcat tacaggcttc tgtggtaacg gtgaaccccg
     5221 gtggcactgg taatccaatc cgcaccatct cagcaaggtt ggcgcctttc ccgcccagta
     5281 accctcttac tgcgtcccaa tctttattca cgtactgctc aacctctttg atctggttga
     5341 acaaataaac ccatttctta tctgtcatct tttcctccga gccaggctca aaataacccg
     5401 ttaaaacggg attctgtctc caaagtgcaa ttttaccata cgcactttct ttatttgact
     5461 tattcggtca aatttgattt tcatttgctg aagaactgaa aatcacctac tgtaaatata
     5521 ttgaaaggta aaaaacgtca atttgacttg ataattatgg tacttgataa gcggtatctt
     5581 atgaaaatat taatcatcga cgacgaccct tctgtaaccg acctgctcag attgatactc
     5641 gctcccaccc tggcagaaat ccagcacgcc tgtgacggag aacaaggatc tggtctggtc
     5701 agggagttca agccagatat tgttttgcta gactatatgc tgccgggaat agacgggctg
     5761 gaaacaacgc gtcaaatcag gaagatcacc aaagccccca tcctcatcct ttcggtattg
     5821 gatgacccgc tgaccctggc caaagcgctg gatgcgggag cggatgagta tttgatcaaa
     5881 cctgtttcac gcggtgtgtt gatcgcccag atcaacaacc tggtcaggcg ttatagcgag
     5941 aagagcgtgg atcattcgcc gctgaaggtt tcctggcaaa ctgcattaca ataacgcccc
     6001 cagctctatt ttttcaactc tccttcaatc catcgccagg cttcagatgc actggtgaca
     6061 gaacctttaa tctgttgctc cttgagtaat tccagcagtt caccgataaa tggacctggg
     6121 cttagcgaga agcggcgcat tagatcatca ccattcaaca aggggtcggg gttcagcacc
     6181 tgcggcgatt taaaccaggc ctccatcagt ctagcggcca actttaactg gtacaaccac
     6241 tgctcctgca caaattcgca gccaacgtga gaaacttgct ccgccagtgc agcgaccgcg
     6301 gcatcaacac cgccagccgc ggtggctttg aaataccgat agatctcaag tggttcgggg
     6361 atagagccgc ggtcgataaa tccttgcaga agcgggatgg cagccttaaa atcgcgcacc
     6421 tgcttcattt cctcaaccga aagcgccagc gctcgcacgt gatctcccgg ttccgcctgt
     6481 tcgccaagaa tgacactcaa aaaaagcagc gaccttctat cacgcttcga tgggtttttt
     6541 gctaggtaaa aacgccgcaa atcttcccga aaacgaccca gctcaacaag caacgaagat
     6601 tggtaaaagg cagtctgccg cgcatgcgtt ttcacaccac ataaccaatc cagaatatct
     6661 tccagtgacc ggatgcgcgt aaccaggggc ggaaaattca gcggaattga aagcggaaga
     6721 taaggaataa ttccaaacag catgcacaac tgcagcgccg tatgcacctt gtgcccggag
     6781 aagattttaa acagctcgtc tcgttttcgt tcaagtgata cgcgctgaag cccattcaca
     6841 gcagcacgga ccaggtcaat tatttctggc gcaagggtta accccaaatc gaccgtgtaa
     6901 cgcacagccc ggatgacgcg caaagggtct tctataaaac tgtcccggga aaccacgcgc
     6961 aggcgttttt ccagcaagtc tcgcccgccc ttgaacggat caatgatgtg ctccggctga
     7021 ttgaggtcaa tcgccatggc gttgatggta aagtcacggt gcgcgaggtc ttctagaata
     7081 ttctcccctc gcatgattgc gaaatcgact atcaagtcag gcacctctgc ggagtcgatc
     7141 aagacccggc aaataaggcg cccttcatcc aggataaaat aattgccgtg gttcctattg
     7201 gcaaaagccc gagccattgg ccgggggtct tgtgcgcaga caaaatcaaa atcactgcaa
     7261 ggtttcccca gtaatgcatc acgaaccgcc ccacccacaa ggtagagcgg tttggatggt
     7321 tcggtcaagc tggcgagctg tttaataaaa tcaggaaatt gcaccctcag acccctgttc
     7381 agagccctga tatttttgaa ggtctactac cccgatgaaa ggcaaattac gatagtaatc
     7441 atccaggtcc aatccgtacc caaaaacgaa cttatttgga atggtgaagc cgcgatagcg
     7501 aatgggtact tccacttcgc gccgctcttc tttatcgaga aggacacaca cgctgatgct
     7561 ggccggccgc ctggtggcca aaaaatcgat gaccgctgaa atagtgtgac cgctgtcgat
     7621 gatatcttca actaaaagga cgtgcttatt aaaaatatcc aggttcaggt caagcgtgat
     7681 gcgcacctga ccattgctct ctctaccgcc cacgccatac gaagagacgg ccatgaactc
     7741 aatggcgtgc ggaatggtga ccttacgcat caaatccgtg agaaacagca ccccaccgcg
     7801 caagatacac acaagcacca gttcgccttt tcccgcgtaa tcccgactga tctgcccccc
     7861 gagttcggca atcctgcgct gtagtgcgtc ttccgttatc agtacttcgc tcaggtaata
     7921 gtgataatcc tgcatgtgac ctccagcgtg atcagtcttt tggcacttcg tggtgcttac
     7981 ggcagcgggc ttcatacatt tcagacgcgc cgataatgac aaccgggtca tcatatctcg
     8041 cgggttttcc atcaaccagg cgctgggtac ggctggcggg ttctccgcag accatgcaga
     8101 tcgcctgcaa cttatccacc tgctcggcac gcgccatcaa tgcgggcata cagccgaaag
     8161 gctctccttt aaaattcata tccaatccgg cgattaccac cctgagtcca cgtgtgacca
     8221 tttcttccac aacctcaatg agctcgtcat caaagaattg agcctcgtcc accgccacca
     8281 ccgtagtttc tggtttgagc agcggaataa tctcgctgga agaacggatt ggtgtggcgc
     8341 cgaattcaga gccagcatgt gaagtaacct tttgtaaacc atagcgccca tcaatggctg
     8401 gcttgaaaac ctgaaccttt tgtttggcta tcgttgcgcg acgaaggcgg cgtatcagct
     8461 catcggtctt cccacagaac atcgagccgc atatcacttc aatatggccg gattttctat
     8521 ccaagtttca cctcctaagg gttaaaaacg attctacctt aattctggca ttttgatttg
     8581 aagataggcg gttaataaca aactcccctg gtttttgcca ggggagttga tttgttgacc
     8641 acctagatca caatttcttc tgcggcagcg ggcaatttat accccatctg gcgcagattc
     8701 ttcttcatat caataaggga cttgcgccca aagccttcaa tcgccagaat ggtggcttca
     8761 ccttcagcca acttttcgag caactggccg acattcatca acccggcttt ttccagcgct
     8821 tctgcagctt ttaccgtaat gccaatatcc ttcacggagc gttcaggcga agtcttggcg
     8881 gcaagctttg atttcttctc ttcgacatag tcctgacggg cctgcagttt cttgtaaaag
     8941 cggtccacct gaccttcaat atcgataatg cgttgctgtt ctccggtgaa gaatgggtgg
     9001 catttggagc agacttctgt acgaatttct tttcgggtag aaccggttat ccaggtattg
     9061 ccgcatgagc aggtgacctt cgcttccggg tagtatgttg gatggatacc ttttttcatt
     9121 atcactgacc ttcctgattc tggtttttta caattgtcaa tttctgacgg atttgtaaaa
     9181 aatactttta cttggtttca actggtctga catggacctg tttttgaccg ctcggcaggg
     9241 tttcgtattc cacaatcccg tgcgctacgg caaagagcgt gtcatcggtg cccactttaa
     9301 cgttcagccc tggtttaatc ctggtgccgc gctggcggac gagaatattt ccggagagca
     9361 cctgctcacc ggcgaatttt tttacgccca accgctggga gttgctatcg cggccgtttc
     9421 gacttgaacc accacctttt ttatgtgcca tttcacttac tccaaattga tcgattcgat
     9481 ttttaaacgg gtgtaacgct ggcggtgacc actcttaacc ctgattctct tcttgggaga
     9541 gtatttgaaa gtatccactt tcgggccctt gatgtggtcg acaacggacg tggtcactac
     9601 tgcacctttt accgatgggg tgccaacctg aaccttttcg tcttcgacaa ccagtaacac
     9661 ttcagcgagt ttgacctcgt gacctacctc gacatccaac aaatcgacat cgatcgtagt
     9721 tccttcgacg gcgcggtatt gcttgccgcc actttccact attgcgtact tcatttttaa
     9781 agattctcca atcttcactt caccgttacc tcgcgttctt cggtcgccag ctcgcgggga
     9841 agttggaata gtaacaacct cacagatctc ctgctccggt tgaaaagcag acggtattat
     9901 acaggtagtg cgcgaacctg tcaatgaaga ttatccagca ccatgcgggg aggccagcac
     9961 atcgtataca gttaataaaa aaagactcac ccaaccattt cggcaacaga tttatttagt
    10021 ttgacagtca tcgaaacctc cccgtccgca aacggcgcgg gttgtggagt tttgggttat
    10081 attaaacatc ataaacgaac gtgcttatcg aaaaaacatg cgctcttcaa ctttggaagt
    10141 tcaacttctc ggcgggttta gcgtccgata cgacgggcgg gaggtgcaag cgctgcgctc
    10201 ggagcgtatg gcacttttgc tgtcgtattt ggcgctccac gcggacgcgg cgctcacgcg
    10261 caggcaggtc gcgtatcttt tctggcccga aacgggggat gaacaagcgc gcacgaatct
    10321 ccgcaatctc attcactatt tcaaaaaagc cctgcccgaa gcggacgcgc tgttggagtt
    10381 ggacgggcag acgatccgct ggcgtgacac ttcaagcctg agcgtggacg cgctccagtt
    10441 tcgagccgcg ctggccgcca cccgcgccgt gcaggacgac gagacacgca tccgccattt
    10501 gcaggaggca gttaatcgtt atcgcggcga gttgctgccc gggcattatc aggattgggt
    10561 gctgttgata cgcgaggagt ttcaacaggc gtttttagat gcgctggcgc agttgacgaa
    10621 gagactcgaa gaggtgcgcc gctacgagga agctgtcgaa gccgcgaacc gtctcatccg
    10681 cgccgacgcg ttgaatgaga gcgcctatct ccagtccatg cgtttgcgcg cgctgatgga
    10741 cgaccgcgcg ggcgctctgc aggtctatta cgcttgcgcg accactataa ggcgcgaact
    10801 cggcgtcgag ccgtcgcagg aaattcagtc gttttaccag cggctggtcg gtgcggagca
    10861 aaacgcacag gcgcagtttc gggaggaaaa agcaccggag cagatgcggc tggtcggaag
    10921 gaagcaggaa tggagtcgtt tgaaggaagc gtggggttct gtgcaaaaag ggaaagctgc
    10981 gctggtcctc atactgggcg aagcgggcat cggcaagacg cgcctggctt cggaattgat
    11041 gcaatgggcg cggcgtcagg gaatccgcgc ggcgcgcgcc gagtgtttcg ctgcggaggg
    11101 gaacctgccc ttcgcgccca tcgttgcctg gctgcggacg cccgaaatca gggaggagcg
    11161 ggcagggctg gaagcgttgt ggaagcacga actggcgcgc ctgctacccg aggacgcggc
    11221 gggcggaacg ccttcgagcg ggcgaaagtg ggaacggcgc aggctgttcg aggcggtggc
    11281 gcggggcttg ctgggatcgg gtgcgccgcg cctgctgatg ctggacgacg cgcaatggag
    11341 cgacagcgag acgctcgaaa tcgtccatta cctgatgcgc tacgacgcca acgcggcgct
    11401 aatgattttg atgacggcac gggttgagga actggacgag gagcatccgc taacccagct
    11461 gcggctgtct ctacaagaac gcaagcaact gcacgaactg gaattgatgc cgctggacaa
    11521 gcccgaattg ggtctgctgg cgcgggatgt gttgggcagg gatttggaga aggcggagga
    11581 ggacgaactc ttccgtcaga ccgagggcaa tccgctcttt atcgtggaga cgctgcgcag
    11641 cgggaaaatc aacgagacgg ggatgccgca aacgctgcgg tcagtgttga cccggcggct
    11701 gggcggactc tcggcggcag cgcgcgaact ggtgggcgtg gcgggcgtca tcgggcgcga
    11761 gttccgctat ccgcttttag cgcgcgccag ccagatgaac gagtccgtgc tggtacaggc
    11821 gctagacgaa ttgtggctgc ggcgcgtcat tcaaatccag cgggcggatg cctacaactt
    11881 tacacacgga aaattgcagg aggtggcata cgaggcgttg agcgcgccgc ggcagcagtt
    11941 gcttcaccgc aagattgccg agtcgttgga ggtggagcgg gaagatcccg ccgttattgc
    12001 gcgtcactgg gatgcgggcg ggcagtctgc gcaagcggtg gaggcttatc ggcgcgcagc
    12061 ggaggcagcg cggcgggtct ttgccaactc cgatgcgctg gcgcatttgg agcgcgcgct
    12121 ggaactgctc aaggaaaaaa tcggcgccga aatggaggag caaaagaaat cggctggcga
    12181 aatatgcgaa gcgctcggcg atatttacga gatgggcaac aaccaaaaat cgctggaacg
    12241 atacagggaa gcgctgacgc tcgttgagtc atcggacggg ctgtcgctgg cgcgcgtctt
    12301 gggcaaactc gcccgtgccg aggcgaatca cggcaacctg caagaggggc tgaatctttt
    12361 cgcgcaagcc gaggcggcgc tcggcgaacc gtcggaggcg gaagactggc agcgcgcctg
    12421 gctgaaggtc cattttgacc gcgtgtgggt tgagtacgac gccaaccggg tggaggcgct
    12481 cgaggccgcg ctggcgccca tccgccctgt ggtggagcgt ctcggtgaat tggaaaagct
    12541 gggcgagtat tatttcctgt tccccaccgc ctacttccgc cgcgacggct atcaaatcaa
    12601 cgacgaaatc atgcgttact ccaaactggc gttggagacg agcgcaaaga ccgacaacct
    12661 ggaattgcaa acgcgcacga acttcggcta cggcttttgc catttcctgc gcggggattt
    12721 cgacgaggcg attcattatc tcggcgaggg gctcaggctg gcggagcaga tcggttacgt
    12781 ggaactgcag ctgatgagcg tcacctatct ggcggcggcg tatcgtggcg cggggcgggt
    12841 ggaagaatgc cagacctgcg ctgaacgcgc actgacgttg agcgagcgcg aaggcttcct
    12901 ggcgtatgtc gccgcggcgc gcgccaacct gggctgggcg gcgtggaggc ggagtaatct
    12961 gcgtcaagca aaggcgctca gcctatccgc gctggagatg tggggcgcgg ggtatccctt
    13021 ccgctggctc gccttgtgga cgctgattga cctgagcttg cactctctgc gtactgatga
    13081 agcgctggag tatgcgcgca agttgaagga gccgcatcag caaatctttg agaaagaagg
    13141 agacgatctg ctcatacaag cctcatctgc agacgtgggg aacgccgcgc ccttgctggc
    13201 acaggcgtta gcgtgggcaa agaagcgggg atacctgtag gtcgaggtgg ctgcgacctc
    13261 ccatcgcatc cacgaattga cacaatcaac ttcgattccc aattcccgaa cagcaaatat
    13321 tcaaaaacaa aacatcggag atcgaaaacg agatatcaaa tcccattttc acccctaagg
    13381 ctgctattta aaacgaaacg cttttgaaac gctccatccg tacactatgg atagtcgtca
    13441 aaccatagtt ttacggaggt tttcatgaaa gcgttacgca ttaccctgct cgtcttcacc
    13501 atctacaccg ccatcatcgg cattctgttt ctcttcgctc cgcgcatcgc cgaaaccgcc
    13561 tttcagacgc gcctgcccga cgccgcgctg accatgctct acggccaggt agtgctggtc
    13621 cttgccttgg ccgcctggct gatccggtcg gacgccgccg cgctccgcaa aatggtctgg
    13681 gcgctggtct ttgcggaggt ggggcatgtc gtcgtcttcc tttggcagtt gatgaacggc
    13741 atctcgacct tcgcgcaagt cggtccgccg atgatcatcg ctgcggtctt cgcggttctg
    13801 ttcgtggtct tcaaccgcaa gggataacgc tcatgcgcgt ggaagggaac ccagccgggg
    13861 cgcgaacgca tctcttcacg ctgcggatct gggcggtgaa cgaagggacg gctgcgccga
    13921 agtggcgcag ccgtttgcag aatattcgca gcggagaagt gttcttttgc gagaatttat
    13981 ctgacctgtt gagtcacatc gaagagacat tatgcgaaca ggatcaggac gaggttccct
    14041 gaccgcaaaa tcgagcgccc tgccgcaaga catactctgc caacagtatg gctcacctgc
    14101 cacccgtcaa agcgagccag ctaactctga aaactacttt tctaccaaat agccgtcgcg
    14161 ggcaaacgcc gaagccggtg cgctgcagac gatgttgggc agagatgctg tatattcgat
    14221 gtgtctcatt gtgcactgat tttttcttgc aatcgaaacg acttgggcgt ttacacagaa
    14281 tagataaaaa ttcattgagc aaattttacc cactctttct ctcaaggtga atgtctttac
    14341 tgcttttata aaggaaaata atcgttcagt aacactatgc tttggtttga cagttgcaga
    14401 tgaaccagca cctgaggggg tcagcaccta ttattcagca ccgagtgaag tgtttttggg
    14461 ggatttctat ttcaacacgc gcccttctcc aacccgttgc gtcacccctt tacggtccag
    14521 gaactcgagg aaagcctgag atacttttcg atttgtattg aagtgatcgc ggaactgagc
    14581 cagcgtcaat aactccccac gggaacaacg ctcgatgacg taacccagca tcagaccata
    14641 ttcagccttc cgcaccacca cttcggttga taactgaaca agcgcccctc gctccacgag
    14701 tgattgaaag acatcatctg ttaacaggtc cttgctctct ttcactcctg ggggtaaaaa
    14761 aggctgctga tcgagtatag ctgtgaattg ctccaacctc cgcgattgat tgctggaata
    14821 cctgacttca aagccgggca gggcgatggc tccatcctgc agcttcacca ccttctcttt
    14881 aagccattct cgcaggcata aattcaactc gtcctgtgag attctcaacc gccgggcaag
    14941 ctcatccacg ttaatgccgc ttttcagcgg attctgttga tggtagtgct gaagcaattg
    15001 tacagatttc gcggtcaact ctgcccactt cgcccgcggc aaatacccgg ttgtttgatc
    15061 cgtaagccct gctaacacgc tcacctgccc tcgcccggtc aactcagcca gtatctcacg
    15121 cacgcgctct gaatcggtct cgagccggtt ttgaatctca tcaacagtaa tgaacggttt
    15181 ttccatgagg agcgccagta tgcggttttc aagcgcagta ccggagttca gagatagccg
    15241 ttcgatcaca cttgaatcct gcaacctgta ccgtctgcct ggatgagggt tgacgaccac
    15301 tcccccgccg agcgtgtgcg cgggagacat gcgccggatg atgaatcgat cttctttctc
    15361 cagcaccatt tctttttcaa actcaatttg cacccaaccg ttctcacctg gcttcaattc
    15421 ctggccctgc aaaaccctaa ccctgccagc gcattctgat gtcaacgcaa acaccttcac
    15481 caagtcatta tgcttgagtg aaactggcgc gtcttgtaat aattcaattt gcgcatcaat
    15541 gcgttgagtg ggttccaaca atcgaggtaa ggtgatgacc tctcctcgct tgatgtcagc
    15601 cacatcaacg ccgctgaggt tgaccgcagt acggctgcca ggcagcgcgg tttcctcgct
    15661 gcgcttgtga gtttgcagcc cgcggatcct tgccgaacgc cgggaaggca aaatttcaac
    15721 ctcattgtca acgcacaaag agccattgag cagagtacca gtcacaaccg tgccaaaccc
    15781 ggttaacgaa aaaatacggt ctatgggcaa cctcggtcga cccagatcaa ggcgcggcgg
    15841 aagaggtgcc aatacttctt cgatcgccgc cctcaactgt gcaatcccgt ctcctgtcac
    15901 ggctgatacg cgcaacagcg gcgcgttctc caggcaggtt gacgatagtc tctcgcggat
    15961 atccatttcc accagttcca accacgccgg atcatccacc aggtcaacct tggtcagcac
    16021 gaccaaaccg gtattaacgc gcaggagttc aatgatggcc aggtgttctt tggtttgcgg
    16081 catgatacct tcgtccgcag caattaccag caataccgcg tcaatgccac ccacgcccgc
    16141 cagcatcgta tcaataaaat ctcggtgacc cgggacgtcc acgatgccaa tttcgccctc
    16201 tgattgggat tcaaaccagg cgaatcccag ttcaatcgtc atctggcgtt ttttttcttc
    16261 cttcagacgg tctgggtcaa tcccggttaa ggccttgacg agggttgatt ttccatggtc
    16321 aacgtgcccg gcagttccaa tgacccgcat cactcaacct tttttcaaag ctgtgcgctc
    16381 gtaagaagtg atgtattcat tggcaagcat caacagacct tttattctct tttcagtttc
    16441 ctcgtgaatc acttggacgg tatccaacag gttttgtacc gcatcttcaa gcgaagttat
    16501 ccgttcatct atcttgttga tctcgcggtt cccttcacgg ttggtttctt cctggcttaa
    16561 ggaataattg gtccagcgtt tctggtcgtc cgctttaaat gcaatccatt cctgcctgaa
    16621 tctttcttcc gtcaggcggt tcatttcagt gatttcgttg attctccgtt ccagtctctg
    16681 gttgattacc tcaaaatcgc tttgcccctg tttaatttct cgattaagcg tttccagttc
    16741 gcgtacctga ccatggaagt tttctgacaa tgctttcatt tgctgtattt cttcctgcca
    16801 gtccttccaa aggttttctc tatctactga taagagagac tgtttttcga taaactcaat
    16861 ttgctcctgg ttcctggttt tttctcgatt caccaggtcg tggatctgac cttccagctt
    16921 tttgagatac tctttttggg catcagatag atttcgatcc tcatcgatgc gtttgcgcaa
    16981 cgtgttcaac tcaatctgta aatccgatag tctttttgac tctacacgat agttatcatc
    17041 caggatcttc gccaacctgt tctgttcatc acccgaagaa cgcaggtcag caagctgcct
    17101 agcaagatct tccagctttt gattaagccg gaagtcatct tctcccttgg actgcacgct
    17161 ttttttcaat tcgttaagag ccttaatttc gccctgcagt tcgagcattc ttttattgta
    17221 ggtatcgcgg tcatcgcttc tttgcgctgc agtggtttgc tccattgtgt cgagccgttt
    17281 ttcaatcaat cgctggcttt tagccaattc cgcttttagc ttcgctttcg tctcttcgta
    17341 ttcatctaaa cgcaccacac tggaaaaaga cttcttgtta tccttattga tgttggacaa
    17401 ctctcgtttt tgcaatgcga tcacttcttg cagcttggaa acctgcgcct gcagctcaat
    17461 aatcgtttgc ctgtctttac ggcgctctcc atccagccat tcaattcgtt ttacgatttc
    17521 ttcattttcc atttttcaac ctttcttcac tgcgtgggca tttattttaa taagggtaga
    17581 tctgccgtaa atggtttaat gatgccctcc accaattgcg gaaataaacc tgcaaggatg
    17641 agcacaagaa taaccgtaca gataatgata acctgagtaa cgctttcatg gatacgccaa
    17701 ttatttggcg ctggtaaagt catgactctg aacaaccgga ttacagcaaa gattaaccct
    17761 gaaaggccaa atactgtcca aatcaccgac ggcaccgaaa tgtgacccaa ctggtaaagg
    17821 agttccagcc gaattgggaa agcggcaaaa agaggaaaac ctgaaatagt aaacaaagag
    17881 atcaaaaaac cgctggcagc aaatgggtat tgccttataa ccccttggaa accgtcgtga
    17941 gtaagggcaa ttcccgcctg gtgaagaatg gataaagagt acaccatcag tgccaggctt
    18001 aatacgcggg gaaaaagtga atgatacatc aggatcaaac cctgctctga tttaaaactg
    18061 atcatgatta atgaaaaacc tgtttccagc agtacacagt aacccaacac acgtttcaca
    18121 tctctttcta cgctggccca caacccggtg gtaacaatca tgatgatacc cgtgctgctg
    18181 agaactggag caagataaga ggctgaccgc aaccaaacca ggctggagat atagttgact
    18241 gcgacaaaga gaaccacgat cgggaaaaga ctgataatga aaccagacag atacggctga
    18301 acggcgcgcg aaaattgtgg gacccaggag tgaaaaggaa aaactgccag ccacaaagaa
    18361 aatccaatca tcaacgtgat ggccgctccc agcaaattcc cctgttcaga agggctggcc
    18421 tgggttcctc ccagtatcca ccccgaaaaa aggataaatg gcatcgcgag tgtcagataa
    18481 accagaaaac ggataacacc tttctcctga ttcatctgat tgtctttgac cagaacgatc
    18541 atcagcagca cagccagttc aaccagcacc gcggcgtaaa ggaagggttg aaccgccaga
    18601 gccgccacca ggatggcgct aatcgcgaga gaaagtggcg caaacttggc tggaacagta
    18661 tgggtattcc acaaaaaagt atagatgaac aaaatcccaa acaacccggc caggataaaa
    18721 cggtcgccgt tatcaagatg gagtgccctg cctaaaaatt gaagatccgg gcggatatcc
    18781 aggaccatgc cgccaagacg caatacattg ccgatcggtt gaaaaaacgc gatgagggcc
    18841 aatagaaggc taaccacgat gcctgttaat ttcaatatcg ttggatattt caacaagaa
//
LOCUS       DAOTLW010000034        18332 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_3451_length_18332_cov_15.432511, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000034 DAOTLW010000000
VERSION     DAOTLW010000034.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18332)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18332)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18332
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3451_length_18332_cov_15.432511"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..222)
                     /locus_tag="P5516_06440"
     CDS             complement(<1..222)
                     /locus_tag="P5516_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92112.1"
                     /translation="MKKIAIPFKNLTYEAKLNLFFAGLVTFYALLVGQELMYKNICGG
                     LSYDFCAYWSGGRIMNEQGYRYIYDADILL"
     gene            588..1340
                     /locus_tag="P5516_06445"
     CDS             588..1340
                     /locus_tag="P5516_06445"
                     /inference="COORDINATES: protein motif:HMM:NF020123.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:720..722,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HRT92113.1"
                     /translation="MALKTGSPAPDFTLPSQLGTNITLSDYRGKNVVLAFFPLAFTPI
                     UTEQIPLYEAEMASFAGLDTQVLGISVDSVPCLTAWAKDLGGITYPLLSDFWPHGAVA
                     QQFGVLRQEGYTERAIFILDKQGVIRYVDVHDIAAQPDNDELRRAIREIDPAVRHRPE
                     PPAPEPAALPHGGIVMYCTKWCPDCKDARAWLARHQLPYTEVDITTTPGAAQQVQRWA
                     GGNRTTPTFDIDGTIVVDFDLPRLRQVLKISD"
     gene            1411..1845
                     /locus_tag="P5516_06450"
     CDS             1411..1845
                     /locus_tag="P5516_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcgN family cysteine cluster protein"
                     /protein_id="HRT92114.1"
                     /translation="MPPPFWKTKRLDELTREEWESLCDGCARCCLIKFQDEDSGHLYL
                     TNVVCEYLEIYHCRCTRYAERSTLVPTCLTLTPALVGLLSWMPETCAYRLLAEGKDLP
                     AWHYLVCGDRKAVHSAGASVRGKVVSARDVAEEDLPDYVVEE"
     gene            2033..2989
                     /locus_tag="P5516_06455"
     CDS             2033..2989
                     /locus_tag="P5516_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HRT92115.1"
                     /translation="MIVTTKELFKAAYGKYAIGAYNINNLEQTMGLFQGNLDSAAPFI
                     IQISKGARAYSNKLMLEGLIRSADQVFPRAIFAVHLDHGDEETCYDCINSGFYSSVMI
                     DASAEPFEKNIEITRRVVEAAHAKGIVVEAELGKLGGVEEHVQVEEANAMLTDPAQAR
                     EFVERSGCDSLAVAIGTSHGAFKFSGTQSLHFDVLEQIQKQLPGFPLVMHGSSSVPQD
                     EVERINKAGGNLKGAKGVDADQFKRAAELGVTKINIDTDGRLVWTRVHREYFIEHPEN
                     FDLRPVGKIFMSEYAKFIAHKNVKLGSAGQLENVRALVGGSL"
     gene            3218..7708
                     /locus_tag="P5516_06460"
     CDS             3218..7708
                     /locus_tag="P5516_06460"
                     /inference="COORDINATES: protein motif:HMM:NF028237.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HRT92116.1"
                     /translation="MPVIEASVYTEFILTVAAVILTLVVVVYAGFHRKINGALHLAIL
                     SSLGTIWGVIYLIELLTKDIERKALLDNLQYIPVGFLPPVFFVVIMVFTGRAAVLHKP
                     QLALMIFVIPVMNIFIIATSKHHQLFQLTAGGHDGSLIPPEHLHGPWFWVLVAHGFIM
                     MFAAVALLLFSHLRAPHWARARLGVLLLASLVITAAAILSLPMWMENFTDLITLTALL
                     IAIIVITYGLLGNRLLHAIPLAASTLLSQLSDAVIILNAHGEILDLNPAAANIPSLLL
                     KEKIGQSFPEVIKTGAGIDLGADWISTYPHEIALGQSSDLHAYDMRISPLIEAGDTAA
                     GSMVVLRDITLRKREEVEKAQFENRYRAIFENTSYGILLLDSQGRIIDSNEQFVLLSG
                     YTPDLLRAYRLKEIISLAPDLAEISVDGARDDPFAYQEAALLLANGDYLPVEMNITPI
                     SAAGGTAYFVTLQDIRDRKRAETATRSALENAQLRADELATLRVMGEALNQATSLRHA
                     LLPVFATVRDVTKSSSIWLYLLGSGPSGHQRYEYHPREAINQLSSEYMHSRVPKCLSR
                     LLEDSLIGPREITDCRCSTLTSEGKHYAFPLYISNRPLGILNFIEEPGSPVNENKLKL
                     LQTICDSLAVAVERVRLFKSEHDQRKLAETMRDIGTALTTSLDINEILDLLLDQLSRL
                     IPYDGGNVMLVEDGEVVIARSRGYELSQKKNAAHLKGLRFKIQTTPNLERLFATQMPV
                     IIPNTAQEKTFVPTSVSPDYHAWLGAPVITDGQVNAVFSLDKVEAGFYTDEHARLLGA
                     YAAQASLAIKNARLYSAEKRRIKELDGLRATLTDITQQLDVNTLLKEIVKRAMRLLSG
                     EVGELGLYDPETRLVRIVVSENFKPDTMGLVVRRGEGLMGRVVKTKKAVAVPDYSLWG
                     GRLLEYEDQRLYSALAVPMLSAEGEVLGVLAVGFTSHEHRFSENDTRLINLFAQQATV
                     ALRNARLYEEARRRAEEAETIREAGAAVVSTLNQEKAVELILQQLVQVVPYDSASVLL
                     YNKGKLKIVGGHGFKDIRAVLGMEFALDRNNPGAVVFLEKKPLILTNIPEQVPHFNHL
                     TGHNHIICSWLCVPLVIQNQAIGVLALDGHSVNQFNKEHERLVIAFADQVAIALENAR
                     LYENALQSASRFEILYKVSQLISANIRSEAIYPAIHEATSELMETEFFSISLVKEKEG
                     LIEDVYMVDSGKPGELSSRPLDQGLFGKVLKTGQSIHFNTFEESMLEETGAILFGDSE
                     EEQISQSILVVPLKIGVRLVGVISTQTYKPYAYSETDLELLELLGANAAIAIENARLF
                     DAVQELAVTDPLTGLFNRRKLIELGESEFTRSIRYERELSAVMLDCDYFKRVNDTYGH
                     VVGDQVLRRLAELCTSCLRSSDILARYGGDEFMILMPETAATSAFKAAERLRRIVSST
                     SFETNAGPLTFSVSVGVATLNKSCKSLEQLLERADFASYVSKDAGGNRVTRWTASISR
                     KG"
     gene            8063..9289
                     /locus_tag="P5516_06465"
     CDS             8063..9289
                     /locus_tag="P5516_06465"
                     /inference="COORDINATES: protein motif:HMM:NF024850.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HRT92117.1"
                     /translation="MKALKLFTLFVLFSLVISACQTAGTAETPAAEAAAPAECAADAF
                     GCAVFKPGEVVKIGMGAPMTGGDASYGIDISSGGFIAIDDFGDLNGFKFELVAEDDGG
                     TPEGGAAVANKFASDPAVVAVAGHIYSGATSAAMPIYAKAGIPLLSPSASLPELTTMG
                     SPVFNRIVFTDARQGPPAAEYLYNKLNVRKLAVLHDGGDYGKGLAEAVAAHFAELGGE
                     VVATEALTKGESDYTAVLSAVAAKNPDALYFGGYTAEGAIVVNQMKQTGLEKAIFFGD
                     DGTYGTDFLARTGANGEGAFATISPIPPESPEKEAFDAAYLAKFGKEAGSLSPYTWNG
                     YDATAALIHAVKSVAITGSDGNLYVPRGALVTAVRTLTDYKGVGGTYTCDAVGECNTL
                     PPMFVVVKDGEWVPAP"
     gene            9364..10461
                     /locus_tag="P5516_06470"
     CDS             9364..10461
                     /locus_tag="P5516_06470"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HRT92118.1"
                     /translation="MNEPSLNKINLKSNRFLKVLRPVLGAVIIIFILYRLNQVLLVQR
                     EYGPETWMRLLIAGLVIGGVYALIAIGYTLVYGILFMINFAHGEVMMLGAYSCFFVFE
                     AFKAIHVPSGESFANAYPAVAILIGFIVGMGVSATMGFLLEKIAYRPLRKAPRLVPLI
                     GAIGASIFLQNAAQLLFSPQLRDVANPEVMRRGTGWTVLINGSNVIIPYTGVFTFVLS
                     FLLLAGLYLVVRKTSLGRAMRAVALDKKTAALMGIDVDKTISRTFLISGALAGAAGVM
                     WGLHNGLVYYFVGFIPGIKAFTAAVLGGIGNLPGAMLGGFFLGIIESLGPAALGIDFQ
                     LKDVIAFALLIIILIFRPSGLLGEVLAEEKV"
     gene            10458..12017
                     /locus_tag="P5516_06475"
     CDS             10458..12017
                     /locus_tag="P5516_06475"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92119.1"
                     /translation="MKKPFLQSLRVGLIFGIVHIFLVLIGFNSLVGTLLARISGAALV
                     HSLPPARFMVVYALLLALWAGFGAVRQARAKTLGGSLAHGAAAGLASAALAFVFSLGL
                     SWLLIHRVDFRQYLAVLSFDFIRVTLFNLAPLNGALAVAGVFLAGATLGGLLTGVFNL
                     ALVRSGLQQVKGFLAALQQRFSSLTHGKYRLVFLILGLVLLAAAVILLPLKWGSYWNY
                     VTGTVGLYVVLGLGLNIIVGLSGQLVLGYIAFFAIGAYTMALLNSPFPHNLMWGFWPA
                     LVLGVALAALAGVLLGLPLMRLRGDYLAIVTLGFGEIIRILLKSDILTNFTGGPRGIQ
                     NISGPTLFGRVFNSDVHFTYLITAAVLLAAFVYHRLQYSRTGRAWLSIREDETVARAT
                     GVNTTRYKLLALALGAAFAGLAGAIFASRNQFTGPEEHSLMASINVLSLIIVGGMGNI
                     PGTILGAFALKGLPEILREFANYRLLTFGALLVVMMLSRPNGMIPSKRPKYQVPAESA
                     EDRPQEARHDG"
     gene            12007..12816
                     /locus_tag="P5516_06480"
     CDS             12007..12816
                     /locus_tag="P5516_06480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92120.1"
                     /translation="MTDNVISVRDVTKLFGGLTAVNRVSLEVPEGAIFSIIGPNGAGK
                     TTLFNCISGFYTPEHGEVLFLGDSICGRGTDAIASVGISRTYQNIRLFSNLTALENVM
                     VGLHPRLRETWLDAVLHSPRFKREEDASICEGLKWLAFISLQDHANDLAGSLPYGAQR
                     RLEIARALAGKPRVLLLDEPTAGMNPHETEEMIALIRRMRAEFGITIILIEHDMHVVM
                     GISDLIAVLDFGEKIAEGAPAEIQKNERVIEAYLGPGGAALAKKYRGRKSA"
     gene            12813..13532
                     /locus_tag="P5516_06485"
     CDS             12813..13532
                     /locus_tag="P5516_06485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92121.1"
                     /translation="MILNVAHLNVHYGPIHALKDVSFTLQQGEIVALIGANGAGKSTS
                     LNAISGILRPTSGSITYRGEEISAITPQEIVRRGLIHVPEGRRIFASMSVWENLEMGA
                     YGQPDRAAVKEHMEAMFTRFPRLRERRNQAGGTLSGGEQQMLAIARGLMANPQVLLLD
                     EPSMGLSPILVEQIFDIIREINESGTSIILVEQNALMALSIADRGYVLDTGRVALEGS
                     ADDLLHDPMVISAYLGGNNGQ"
     gene            13797..14546
                     /locus_tag="P5516_06490"
     CDS             13797..14546
                     /locus_tag="P5516_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92122.1"
                     /translation="MFYLIGIDGTDSPSVNGGEDTASLGIRLGLLLQERRFGRMLAVT
                     RHQLLRRDALPFTAENQACCLLMEADADARRDIELTSREFILRQSAPASDPGFALAAW
                     SNLSAAVDTWGQQAKYLRLQRDAAMQLARAHDISVAGFQGNGNGVIGALAAVGLRSSG
                     NDGQFAWLPGLESLKGTLTLPALLNYCAIDRVENLRGRTPFDRDLIHLGEAPVVLLRG
                     GQSLLLLHSCAGEDVNQWRVYSPVELAEVAP"
     gene            complement(14555..15376)
                     /locus_tag="P5516_06495"
     CDS             complement(14555..15376)
                     /locus_tag="P5516_06495"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT92123.1"
                     /translation="MVSKIDRERMQVNRRYWDEVVPVHAASEMYDVQGFKAGRLTLDD
                     LERGEVGEVQGKTLLHLQCHFGLDTLSWARLGARVTGVDYSPQAIDMARALAAECAID
                     ARFICCNLYDLPRQLEGHFDIVFTSYGVLCWLPDLREWARIAAAYVQPGGFFYIAEFH
                     PFAYVFDDEASSLQYRYPYFMRAATRFEFDGTYAEKTARLQNREDYEWTYRMGEVVTA
                     LIEAGLRVEFLREHPFTVYEQLPFLEKEGKARWRFAGGAEPIPLMFSLKAVKALD"
     gene            complement(15430..15951)
                     /locus_tag="P5516_06500"
     CDS             complement(15430..15951)
                     /locus_tag="P5516_06500"
                     /inference="COORDINATES: protein motif:HMM:NF012241.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HRT92124.1"
                     /translation="MSNSEFPVHRPFRGNRLALRPLFNREGETPMLAIQNEMNRMFDE
                     FFSDAFSFPGQFLTRRSAEFFPRMDISETETEFKVSAELPGMDEKDIHIKLEKDVLKL
                     CGEKKSAVEEKENTYHRVERSYGSFERVIPFNTQLDEDKVSAVFKNGVLTVTLPKAKE
                     VIKTSRTITINPS"
     gene            complement(16154..18253)
                     /locus_tag="P5516_06505"
     CDS             complement(16154..18253)
                     /locus_tag="P5516_06505"
                     /inference="COORDINATES: protein motif:HMM:NF024938.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HRT92125.1"
                     /translation="MTSSLKPFFEPAGVAVIGASANPRKLSFGIMRNLVSSTYAGGIY
                     PVNPGAEKILDKPGFSDISAVPDPLELAVIALPAPVIAETLEACGRRGVKAVIVISGG
                     FKEVGEGGQALEARCLEIIHRYGMRMIGPNCVGLMNLHNGLNTTFIHGVPDAGGIGFM
                     AQSGAVLGGVVDQVLGKKIGFSYFISMGNEADVTETDLMEYLGEDPRTRVIATYVEQI
                     KDGRRFLETARRVSRVKPIVLLKGGRSSAGARAVSSHTGSLAGSYAAYQAAFRQAGVV
                     EVESIGALFETALAFDFQPLPAGRRAVIVTNAGGPAALASDALSANGLVLDELGEKTR
                     AVLRAALNPAAQVSNPVDILGGAEPPDYALCTSTLMKDEQVDIVLPLLVPQALVSPAG
                     VAQAVADASAGREKTVLACVMGDISVDEARLVLHEHRIPMYAQPESAGAVLKAMLDYK
                     DWLANPVVPPPALAGIERKAGREVLKAAGGKASLGEVETRPLLAAYGIPLVEGGFAAN
                     SAEAAAVAARIGYPVVMKIVSPQLLHKSDAGGIKLNLADEAALRQAYSAMMTEINAAR
                     PDALLEGVMVERMAPKGQEVIIGMRRDPGFGPLMMFGLGGIYVELFKDVSFRVAPLSA
                     ADARQMITETRAGRLLTGFRGQPRADLDAVVDVLLRLSRLALDHPEIQEAEVNPLLVF
                     PEGQGALALDGRVILGN"
BASE COUNT         3704 a         5667 c         5027 g         3934 t
ORIGIN      
        1 gagcaggata tcagcgtcat aaatgtaccg atagccctgt tcattcatga ttcttccgcc
       61 gctccaatac gcacaaaaat cataagaaag tccaccacaa atatttttat acattagctc
      121 ctgtccaacg agaagagcat aaaaagtgac cagccccgcg aagaataaat tcagtttcgc
      181 ttcataggtt agatttttga agggtatagc gatttttttc ataatgacct ttcggctgtg
      241 aacctgttgg atcgcaatat taataattgt cgaaaatctt cttacatatt gcgcgtcttc
      301 taagttcctc gattgccatg gacacggctc tacattacta tccccatttt ataccttttt
      361 ttcccagcgt acccaatttt ctggcttgca atcaattgtc atcgttcaat caatgatgaa
      421 ggttttctta ccgcttttct ccagttcccc tccttctccc tttcgcgcct gcccctcccc
      481 gttcaatcgt aattcaccgc gggtctggtt cgcccccgcc ctccgcgcgc cgtacatcag
      541 gtcgcccgcc aatcaatggt aaaatcagcc cattggaggt aaaccagatg gcacttaaaa
      601 ccggctcccc cgcacctgat ttcaccctgc cctcgcaact gggaacaaat atcacactca
      661 gcgattaccg cggcaagaat gtggtgctcg cttttttccc gctggctttc acgcccatat
      721 gaacggagca aatcccgctt tacgaagccg aaatggccag tttcgcggga ttggacaccc
      781 aggttctggg tatcagcgtg gattccgtcc cctgcctcac cgcctgggcc aaagaccttg
      841 gcggcatcac ctacccgctg ctttctgatt tttggccgca cggcgcggtg gcgcaacaat
      901 tcggcgtgtt gcggcaagaa ggctacaccg aacgcgccat cttcatcctg gataaacaag
      961 gcgtcatccg ttacgtcgat gtgcacgaca tcgccgcgca gccagacaac gatgaactgc
     1021 gccgcgccat ccgcgagata gaccccgccg tgcgccaccg ccctgagccg cccgcacccg
     1081 agccggcagc gctgccccac ggcggcatcg tgatgtactg caccaaatgg tgccccgatt
     1141 gtaaagacgc gcgcgcctgg ctggcgcggc atcaactgcc ttataccgaa gtggatatca
     1201 ccaccacccc tggcgccgcg cagcaggtac agcgctgggc gggcggtaac cgcaccactc
     1261 ccaccttcga tatcgacggc accatcgtgg tcgattttga cctgccgcgc ctcaggcagg
     1321 tgctcaaaat ttctgattga gcgccaatca catcccgtta tggcaggggc gcggccgcgc
     1381 ccctgcgttg tattccggta taatcacccc atgcccccgc ccttctggaa aaccaaacgc
     1441 ttggatgaac tgacccgcga agaatgggaa tctctctgcg atggctgcgc gcgctgctgc
     1501 ctgatcaagt tccaggacga agacagcggg catctctacc tcaccaacgt ggtctgtgaa
     1561 tacctcgaga tctaccactg ccgctgcacg cgctacgcag agcgttccac gctcgtgccc
     1621 acctgcctca ccctcacgcc agcgctggtc ggcctgctct cctggatgcc cgaaacctgc
     1681 gcctaccgcc tgctggcaga aggcaaagac ctgcccgcct ggcactacct ggtttgcggc
     1741 gaccgcaagg cggtgcacag cgccggcgct tccgtgcgcg gcaaggtcgt ttcggcccgc
     1801 gacgtggcgg aagaagacct gcccgactac gtggtggaag agtgaagttg ccatctcttt
     1861 tttgcccgtt ggtttgaggg ccagagcaac ccgcggcgag ggtcaggctg aaggctcatg
     1921 ccccgcgccc acccgcccct tcatcagcgc aacgccgctt cgatagtgag cccgtcggtg
     1981 ctataattaa gccagagata accttccttc cggaagtacc aaggagagcc ccatgatcgt
     2041 caccaccaaa gaacttttta aagccgccta cggaaagtac gccatcggcg cctacaacat
     2101 caacaacctg gaacagacca tgggtctctt ccagggcaac ctcgacagcg ccgccccatt
     2161 catcatccag atcagcaaag gcgcccgcgc ctactcaaac aagctcatgc tcgaaggtct
     2221 catccggtct gccgaccagg tcttccccag ggccatcttc gccgtgcacc ttgatcatgg
     2281 agatgaagag acctgctacg actgcatcaa tagcgggttc tattcctcgg tgatgatcga
     2341 cgcctccgct gaaccttttg aaaagaacat cgagatcacc cgccgcgtgg ttgaagccgc
     2401 gcacgccaaa ggcattgttg tcgaggctga actgggcaaa ctgggcggtg ttgaagagca
     2461 cgtgcaggtg gaagaagcca acgccatgct caccgacccg gcccaggcgc gcgagttcgt
     2521 agagcgcagc ggctgcgatt cgctggcagt tgccatcggc accagccacg gcgccttcaa
     2581 gttcagcggc acccagagcc tgcatttcga cgtgctggag cagatccaaa agcagctgcc
     2641 gggcttcccg ctggtgatgc acggttccag ctctgtgccg caggatgaag tggagcgcat
     2701 caacaaggcc ggcggcaacc tgaagggcgc caaaggggtt gacgccgacc agtttaaacg
     2761 cgccgccgaa ctcggcgtta ccaagatcaa catcgatacc gatggccgcc tggtgtggac
     2821 ccgcgttcac cgcgaatact tcatcgaaca ccctgagaac ttcgacctgc gccctgtggg
     2881 gaagatcttt atgtcggagt acgccaaatt catcgctcac aagaacgtca aactgggcag
     2941 cgccggtcaa ctggaaaacg ttcgcgcgct tgtgggcggc tccttgtaac ctgctcgcga
     3001 gggggctgtt cacctggtaa atgatcacct ggcgcgcttg aagcgcgtgc gcgccaggat
     3061 tcaccacctg aaggggggta atgaatgagt ttagaatatc ttttcagtta cctcagaaac
     3121 cctgttcgtc gaagttcacc ctatataatc agggtggaag aacagggaac gtaggaatga
     3181 caatcatttc aacctggtaa attttgggct gacagcaatg ccagtaatcg aagcgtctgt
     3241 atatactgaa ttcatcctca ccgtcgccgc ggtcatatta accctggtgg tggtggtgta
     3301 cgccgggttt catcgcaaga tcaacggcgc gcttcacctg gccatccttt cctcgctggg
     3361 gacgatctgg ggcgtcattt acctcattga actattgacc aaagacattg agcgcaaagc
     3421 gctgctggac aacctgcaat atataccggt cggttttcta ccaccggttt tctttgtggt
     3481 gatcatggtc tttactggcc gcgctgctgt acttcacaaa ccgcagttgg cactgatgat
     3541 atttgtgatt ccagtgatga atattttcat catcgccacc agcaaacacc accagctctt
     3601 tcagttgacc gccggcggtc atgatggcag cttgatacct cctgaacacc ttcacggtcc
     3661 ctggttttgg gtattggttg cccacggctt tatcatgatg tttgctgccg tggctttatt
     3721 gttgttttcc caccttcgcg cgccacactg ggcgcgcgcg cggttggggg tgttattgct
     3781 ggcctctttg gtcatcaccg cggctgccat cctttcacta cccatgtgga tggaaaactt
     3841 caccgatctt atcaccctta ccgcgctgct tatcgccatc atcgttatta cctacggttt
     3901 gctgggcaac cgcctgcttc acgccattcc gctggcggcc agcaccctgc tcagccagtt
     3961 aagcgatgcg gtcatcatcc tcaacgccca tggcgaaatc cttgatctga atccagccgc
     4021 ggcgaatatt ccctcgctgc tgctcaaaga aaaaattggg cagtctttcc ctgaggttat
     4081 caaaacaggc gcgggtattg acctcggcgc agattggata agcacctacc cgcacgaaat
     4141 cgctctcggt cagagcagcg accttcacgc ttacgatatg cgcatctctc cgctgatcga
     4201 agccggcgat actgccgctg gttcgatggt ggtgctgcgc gatatcaccc tgcgcaagcg
     4261 tgaagaggtt gaaaaagcgc agtttgagaa tcgctaccgc gccatatttg aaaacaccag
     4321 ctacggcatc ctgctgctgg acagtcaggg cagaatcatt gacagtaatg aacagttcgt
     4381 cctgctttcg ggatacaccc ctgacctgct gcgcgcatat cggctgaaag agatcatttc
     4441 cctcgccccc gacctggctg aaatctctgt cgacggcgcg cgcgatgacc cctttgccta
     4501 ccaggaggcg gcgctgctgc tggcgaacgg agattacctg cctgttgaga tgaacatcac
     4561 ccctatttca gccgcggggg gcaccgccta cttcgtcacg ctgcaggata tccgcgaccg
     4621 caagcgcgct gaaaccgcta cgcgctcggc gttggaaaac gctcaattgc gcgccgatga
     4681 actggccacc ctgcgcgtga tgggtgaagc gctgaaccag gccacttcgc tgcgccacgc
     4741 cctgctgcca gttttcgcca cggtcagaga cgttacgaaa agttccagca tctggctata
     4801 tttgctcggt tctggccctt cagggcacca acgctatgaa tatcacccgc gtgaagccat
     4861 caaccagctc agctccgagt acatgcacag cagagtaccc aagtgcctct cgcggcttct
     4921 ggaggacagc ctgatcggcc cccgggaaat caccgactgc cgttgcagca ccctcaccag
     4981 cgaggggaag cactatgcct ttcccctgta catcagcaat cgtccgctcg gcattcttaa
     5041 tttcatcgaa gagcctggct caccggtgaa cgaaaataag ctcaagttgc tgcaaacgat
     5101 ttgtgattcg ctggcggtgg cggtggagcg cgtgcgcctg ttcaagagcg agcacgatca
     5161 acgcaaactg gctgaaacca tgcgcgacat tggcacggcg ctcaccacct cgctggacat
     5221 caacgaaatc cttgacctgc tcctggatca gctttcgcgc ttgatccctt acgacggcgg
     5281 caacgtcatg ctggtggaag atggagaggt agtgattgcc cgttcgcgcg gatatgaact
     5341 atcgcaaaag aagaacgccg cgcatctcaa agggctgcgg tttaagattc aaactactcc
     5401 aaacctcgag cggttattcg ccacccaaat gccggtcatc attccgaata ccgcccagga
     5461 aaaaaccttt gtacccacat ccgtatcgcc tgactaccac gcctggctgg gagcccccgt
     5521 gatcacggat ggccaggtga atgccgtttt ctccctcgac aaagtggaag cgggttttta
     5581 cactgatgaa cacgccaggc ttttaggcgc ttacgccgcc caggcatcgc tggcgattaa
     5641 aaacgcgcgc ctttacagcg ctgaaaaaag gcgcatcaaa gaactggacg ggctgcgcgc
     5701 cacgctcact gatatcactc agcagcttga tgtcaacacc ctcctcaaag aaatcgtcaa
     5761 acgcgccatg cgcttgctct ccggtgaggt cggcgaactg gggttgtatg atccagaaac
     5821 ccggctggtg cgaattgtag tatcggagaa cttcaaacca gacaccatgg gattggtcgt
     5881 caggcgcggt gaaggattaa tggggcgcgt ggtgaagacc aaaaaagccg tcgctgtgcc
     5941 agattactcg ctctggggcg gcagactgct ggaatacgaa gaccagcgcc tgtacagcgc
     6001 gttggcggta cccatgctct cggcggaggg tgaagtgctg ggcgtgctgg cagtgggttt
     6061 taccagccat gagcaccggt ttagcgaaaa tgatacccgc ctgatcaatc ttttcgccca
     6121 gcaggctacg gtggcgctgc gcaacgccag gctgtatgaa gaagccaggc gccgcgcgga
     6181 agaagccgaa accatccgcg aagccggcgc ggcggtggta tccaccctca accaggaaaa
     6241 agccgtcgag ttgatccttc aacaacttgt ccaggtggta ccctatgaca gcgcctcggt
     6301 gctgctctac aacaaaggca aattaaagat cgtcggcggg cacggtttca aggatattcg
     6361 cgcggttctg gggatggagt tcgccctcga ccgtaacaac ccgggagcgg tggttttcct
     6421 ggagaaaaag ccgctcatcc tcaccaacat ccccgagcag gtacctcact ttaaccatct
     6481 tacaggtcac aaccacatca tttgctcctg gctgtgcgtg ccgctggtca tccaaaacca
     6541 agcgatcggc gtgctggcgc tggatgggca ttcggttaac cagttcaata aagaacacga
     6601 acgcctggtc atcgccttcg ccgaccaggt ggccatcgcc ctggaaaacg cccgcctgta
     6661 cgagaacgcg ttacaatccg ccagccgctt tgaaatcctt tacaaggtaa gccagttgat
     6721 cagcgccaat atacgctccg aagccatcta cccggccatt cacgaagcca cctccgaatt
     6781 gatggagacg gaatttttta gtatttcgtt ggtgaaagaa aaagaaggat tgatcgaaga
     6841 cgtgtacatg gtggatagcg gtaaacccgg cgagctttcc agccgtccgc tcgatcaggg
     6901 gttgtttgga aaggtgctta aaacgggtca atccatccac ttcaacacct tcgaagaaag
     6961 catgcttgag gaaacaggcg ccatcctctt tggggatagc gaagaggagc aaatttctca
     7021 atccatcctg gtggtgccgc tgaagattgg tgtgcgcctg gtgggcgtta tttccacaca
     7081 gacctacaag ccgtacgctt attcggagac cgacctggag ttgctggaac tgctgggcgc
     7141 caacgcggcc atcgccattg aaaacgcgcg cctttttgac gcggtgcagg aactggcggt
     7201 cactgatccg ctcaccgggc tgttcaaccg gcgtaaactg atcgagctcg gggagtccga
     7261 atttacccgc tccatccgtt atgaacgcga gctttcagcc gtgatgctgg attgcgacta
     7321 cttcaaacgt gtgaacgaca cctacgggca cgtggttgga gatcaggtgc tgcggcgcct
     7381 ggccgaatta tgcacctcgt gcctgcgcag ttctgatatc ctcgcgcgtt acggcggaga
     7441 tgagttcatg attctcatgc ctgaaacggc tgccacctcg gcttttaagg cggctgagcg
     7501 cctgcgtcga atagtgagca gcacttcgtt tgaaaccaac gccggacccc tgaccttctc
     7561 cgtcagcgtg ggcgtggcca ccctcaataa aagctgcaaa agcctggaac aactgctcga
     7621 acgcgccgat ttcgccagtt acgtctccaa agacgcgggc ggtaaccgcg tcacccgctg
     7681 gactgcctcc atttcccgta agggttaata acgctcgcgc tcgtctctcg acttaacgat
     7741 tttgcaaggt cgcttgacta tttccataca aagtcgtaac atgttcaatt atcagcgttc
     7801 actatgatta acacattcgc cgtctccatg atgatttcct ttatgatgct cgccgttatg
     7861 cttatttacg cgagcgtttt acgggttggg atggctgaaa tcctcaggta ataaagaaca
     7921 aaagaagaaa accccaaagc cacctgaccc aaaccaggtg gttttttctt aatccgcagt
     7981 gaggaggtgt gatgaaagtc aataaaataa cagcgttctt attcaagccc attcacaatt
     8041 gatagaaact aggagaagaa tcatgaaagc ccttaaacta tttaccctgt tcgtcttgtt
     8101 ttccctggtg atcagcgcct gccaaacagc cggcacggct gaaacaccgg ctgctgaagc
     8161 cgctgcgcct gccgaatgcg ctgccgacgc tttcggttgc gcggtgttca aacccggcga
     8221 ggtagtgaag atcggcatgg gcgcgcccat gaccggcggc gacgcctctt acgggatcga
     8281 tatctccagc ggcgggttca tcgccattga tgatttcggt gacctgaacg gcttcaagtt
     8341 tgaactcgtc gcggaagatg acggcggtac ccccgaaggc ggcgcggcgg tggccaataa
     8401 attcgcttcc gacccggccg tggtcgcggt cgccggtcat atctattccg gtgccacttc
     8461 ggctgccatg cccatttacg ccaaggctgg catccccctg ctctcgccct ccgccagcct
     8521 gccagagctg accacaatgg gttccccggt gttcaaccgc atcgtcttca ccgatgcccg
     8581 ccagggtccc ccagccgcag aatatctata caacaagctg aatgtcagaa aactcgccgt
     8641 gctgcatgat ggcggtgatt acggcaaagg cctggccgaa gccgtggctg cccactttgc
     8701 tgaacttggt ggtgaagtgg tagccaccga agccctcacc aaaggcgagt cagattacac
     8761 cgctgtgctc tccgctgtag ccgccaagaa ccccgatgcg ctttacttcg gcggttacac
     8821 cgcggaaggc gccatcgtgg tgaatcagat gaaacagacc gggcttgaga aagcaatctt
     8881 cttcggtgac gatgggacat acggcactga tttcctcgcc cgcaccggtg ccaatgggga
     8941 gggcgcgttc gctaccatct cccccattcc gcctgaatca cctgaaaaag aagcgtttga
     9001 tgccgcttac ctggccaaat tcggtaaaga ggccgggtcg ctttctcctt acacatggaa
     9061 tggttatgac gccactgctg cgctcatcca cgcggtcaaa tcggtcgcca tcaccggcag
     9121 cgacggcaac ctttacgtgc cgcgcggagc cctggtaacg gctgtgcgca cgcttaccga
     9181 ttacaaaggc gttggcggca catacacctg tgacgctgtt ggcgagtgca acacgctgcc
     9241 gccgatgttc gtggttgtca aggatggcga gtgggttcct gctccatagt catctttctc
     9301 tcctctggtc ggggagggtt tcctccccga cccaccatcc tgaaatgaaa gttgcccggg
     9361 tcaatgaatg agccttcact taataaaata aacctcaaat ccaaccgttt tctcaaggtg
     9421 ctgcgtccag ttttaggcgc ggtcatcatc atttttatcc tttaccgttt gaaccaggta
     9481 ctcctggtac agcgggagta cgggcccgaa acctggatgc gcctgctgat tgccgggctg
     9541 gttattggcg gcgtgtatgc gcttatcgcc attggttaca cgctggtcta cggtatcctt
     9601 tttatgatca acttcgccca cggcgaagtg atgatgttag gggcttacag ctgttttttt
     9661 gtgttcgagg ctttcaaggc catccatgtc ccctcgggcg aaagtttcgc caatgcctac
     9721 ccggcagtcg ccatcctcat cggtttcatc gtgggcatgg gtgtgtcagc cacgatgggc
     9781 ttcctgcttg agaagatcgc ctaccgcccg ctgcgcaagg cgccccgcct ggtgccgctc
     9841 atcggcgcta ttggcgcttc catcttttta caaaacgccg cgcaattgct cttcagcccc
     9901 caattgagag acgtcgccaa ccccgaagtg atgcgccgcg gcaccggttg gacggtgctg
     9961 atcaacggca gtaacgtcat catcccttac accggggtgt ttacctttgt gctctccttt
    10021 ttactgctcg ccggtctcta cctggtggtt cgcaagacca gcctcgggcg cgccatgcgc
    10081 gcggtggcgc tggataaaaa gactgccgct ttgatgggca tcgatgtgga caagaccatc
    10141 agccgcacct ttctcatcag cggcgcgctg gcgggtgccg ccggcgtgat gtggggtctt
    10201 cacaacgggc tggtgtacta tttcgtcggc tttattcccg gcatcaaggc cttcaccgcc
    10261 gccgtgctgg gaggcatcgg taaccttccc ggcgccatgt tgggcggttt cttcctgggc
    10321 atcattgagt cgctcggccc tgccgccctg gggattgact ttcaattaaa agatgtcatc
    10381 gccttcgcgt tgctcatcat cattctcatc ttccgccctt ctggtttatt gggcgaggtg
    10441 cttgcggagg aaaaagtatg aaaaaacctt ttcttcaaag cctccgcgtg ggccttattt
    10501 ttggcattgt tcatattttc ctggtgttga tcggtttcaa ttccctggtg ggaacgctgc
    10561 tggcgcgcat cagcggcgcc gcgcttgttc attcgctgcc tcctgcccgc tttatggtgg
    10621 tttacgccct gctcctggcg ctgtgggccg ggttcggcgc cgtccgccag gctcgtgcca
    10681 aaacgctcgg cggctccctg gcgcacggag cggccgcggg actcgccagc gccgccctcg
    10741 cctttgtctt cagcctgggt ctttcctggc ttctcatcca ccgggttgat tttcgccagt
    10801 acctggcggt gctttctttt gatttcattc gcgtaacgct ctttaacctc gcccctctca
    10861 acggggcgct ggctgtggct ggggtgtttc tggctggtgc cacgctgggc gggctgctca
    10921 ctggcgtgtt caacctcgcc cttgtgcgct ccggtctgca gcaggtcaaa ggctttttgg
    10981 cggcgctgca gcagcggttc agctctctga cgcatggaaa gtaccggctg gttttcctca
    11041 tccttgggtt ggtgctgctc gctgccgcgg tcattttgct gccgctcaaa tgggggtctt
    11101 actggaatta cgtgaccggc acagtgggtt tatacgtggt gctcgggttg ggactgaaca
    11161 tcatcgtcgg tctatcgggt cagctggtgc tggggtacat cgccttcttc gccatcggcg
    11221 cttacaccat ggcgctgctg aactcgccct tcccgcataa cctgatgtgg ggtttctggc
    11281 cggcgctggt attgggtgtg gcgctggcag ccctggcggg cgtgctgtta ggtttgcccc
    11341 tcatgcgcct gcgcggtgat tacctggcca tcgtcaccct gggctttggc gaaattatcc
    11401 gcatcctcct gaagagcgac atcctcacca acttcaccgg gggtccgcgc ggcatccaga
    11461 acatcagcgg tcccaccctg ttcggtcggg tcttcaactc cgatgtgcat ttcacttacc
    11521 tgatcaccgc cgcggtgctg ctggccgcct ttgtgtatca ccggctgcaa tactcgcgca
    11581 ccgggcgcgc ctggctctcc atccgggaag atgaaacggt ggcgcgcgcc accggggtga
    11641 acaccactcg ctacaaactg ctggcgctgg cgctgggcgc ggctttcgcc gggctggcgg
    11701 gcgccatttt cgccagtcgc aatcagttca ccgggcctga agagcactcg ctcatggcct
    11761 ccatcaacgt gctcagtctc atcatcgtgg gcggcatggg caatatcccc ggcaccatcc
    11821 tgggtgcttt cgcactaaaa ggactgcctg aaattctgcg cgagttcgcc aattaccggc
    11881 tgctgacctt tggcgcgctg ctggtggtga tgatgctctc gcgtccaaat ggcatgatcc
    11941 cctcgaaacg cccaaagtat caggtccctg cggaaagcgc ggaggatcgc cctcaggagg
    12001 cacgccatga cggataacgt gatcagcgtg cgcgatgtga ccaagctctt cggcggtctc
    12061 acagccgtca accgcgttag cctggaggtg cccgaggggg ccatcttttc catcatcgga
    12121 cccaacggcg ccggtaaaac cacgctgttt aactgcatct cgggctttta cacccccgaa
    12181 cacggagaag tgctcttcct cggtgattcc atctgcgggc gaggtaccga cgccatcgcc
    12241 tcggtgggta tttcgcgcac ttaccagaac atcaggctct tttccaacct caccgcgctg
    12301 gagaacgtga tggtcgggct gcacccgcgc ctgcgcgaaa cctggctgga cgccgtgctg
    12361 cattccccgc gctttaaacg ggaagaagac gcttccatat gtgaagggtt gaaatggctg
    12421 gcgttcatca gcctgcaaga ccacgccaac gacctggccg gcagcctgcc ctatggcgcc
    12481 cagcggcgcc tggagatcgc ccgcgccctg gctggaaagc cgcgtgtgct gttactggat
    12541 gagcccaccg ccggtatgaa cccgcacgaa accgaagaga tgatcgccct catccgccgc
    12601 atgcgcgcgg aatttggcat caccatcatt ctcattgagc acgatatgca cgtggtcatg
    12661 ggtatttccg acctcatcgc ggtgctggat ttcggcgaga agatcgccga aggcgccccc
    12721 gccgaaattc aaaagaacga gcgcgtcatc gaggcgtacc tcggccccgg cggcgccgcg
    12781 ctggccaaaa aataccgcgg gaggaagtcc gcatgattct caacgtcgcc cacctcaacg
    12841 tgcattacgg ccccattcac gcgctcaaag acgtgagctt tacgctccag cagggtgaga
    12901 tcgtggcgct catcggcgcc aacggcgcgg gtaaatccac cagcctcaac gcgatttccg
    12961 gcatcctgcg ccctaccagc ggcagcatca cctaccgcgg tgaagagatc agcgccatca
    13021 ccccgcaaga gatcgtgcgc cgcgggttga tccacgtgcc cgaaggacgg cgcatcttcg
    13081 cttcgatgag cgtgtgggaa aacctggaaa tgggcgccta cgggcagccc gaccgcgccg
    13141 cggtgaaaga acacatggaa gccatgttca cccgctttcc gcgcttgcgc gaacgccgca
    13201 accaggcggg gggcaccctt tcgggtggag agcagcaaat gctggccatc gcccgcgggt
    13261 tgatggccaa cccgcaggtg ctgctgctgg atgaaccctc catgggtctc tcccccatcc
    13321 tggtggagca gatcttcgat atcatccgcg agatcaatga aagcggtacc agcatcatcc
    13381 tggtggagca aaacgccttg atggcgctct ccattgccga ccgcggctac gtgctggata
    13441 ccgggcgtgt ggcgctggaa ggatccgccg acgacctgct gcacgacccc atggtcatca
    13501 gcgcttacct gggagggaac aacgggcaat agcctatcgg ggggcgcctg aaaccgacct
    13561 tcagcctctg tctcccccaa cagggaagcc cgctttgctt ccccccggaa aggttcaaca
    13621 cgcgcgctgg acggctcttg ctcaaccgaa cgagcgaggg gatttgacat tccctccacc
    13681 atccgctatc attacctcat tccaggagga agctacatgc accacctgac caggggagcc
    13741 gaagggatac tgacatcggg gtccgcttca gcgcgccgtg aacacgtcgg ggtcgtatgt
    13801 tctacctgat cggtatcgac ggcaccgaca gccccagcgt caacggcggt gaagataccg
    13861 cctccctggg catccgtctg gggctgctcc tccaggagcg gcgctttggg cgtatgctcg
    13921 ccgtcacccg ccaccagttg ttgcgccgcg acgccctccc cttcacagca gagaatcagg
    13981 cctgttgcct gctgatggag gctgacgcgg acgcccggcg tgatattgaa ttaacttccc
    14041 gcgagttcat tttgcgccag agcgctcccg cttctgaccc tggttttgcc ctggcagcgt
    14101 ggagcaatct cagcgccgcc gtcgacacct gggggcagca ggcgaagtac ttacgcttgc
    14161 agcgcgatgc ggcaatgcag ctggcgcgcg cgcatgatat ttccgtggcg ggttttcaag
    14221 gtaatggaaa cggcgtgata ggcgccctgg cggcggtggg gctgcgctct tctggcaacg
    14281 atgggcagtt cgcctggctg cccgggctgg aaagcctgaa aggcacgctc accctgccgg
    14341 ccctgctgaa ctactgcgcc atagaccgcg ttgaaaacct gcgcgggcgc actcccttcg
    14401 accgcgacct catccacctg ggtgaagccc ccgttgtgct cctgcgggga ggtcaatcgc
    14461 tgctcttgct gcattcctgc gccggcgagg atgtcaatca gtggcgcgta tattcccccg
    14521 tagaactggc ggaagttgct ccctgaaggc gcgcttaatc cagcgcttta accgctttta
    14581 atgaaaacat caaggggatc ggctctgcgc cgcccgcgaa gcgccagcgc gctttgccct
    14641 ctttctcgag gaacggcagc tgctcgtaaa ccgtgaacgg atgttcacgc agaaactcca
    14701 cgcgcagccc ggcttcgatc agcgcggtta caacctctcc catgcgatag gtccattcgt
    14761 aatcctcgcg gttctgcagc ctggcggttt tctccgcgta tgtaccgtca aactcaaacc
    14821 gcgttgctgc gcgcataaag taaggatagc ggtactgcaa gctggaggct tcatcatcaa
    14881 aaacgtaggc gaacgggtga aactcagcga tgtagaagaa ccctccgggt tgaacgtaag
    14941 cagcggcaat gcgcgcccat tcgcgcaggt cagggagcca gcacaacaca ccgtacgaag
    15001 tgaagacaat atcaaaatgc ccctccaact ggcgtggaag gtcatacagg ttacagcaga
    15061 taaaacgcgc gtctatggcg cactccgccg ccagcgcgcg tgccatgtca atcgcctggg
    15121 gcgaataatc cacgccggtt acccgcgcgc ccaggcgcgc ccatgagagc gtgtccagtc
    15181 caaagtggca ttgtaaatgc agcagggtct tgccctgcac ctcccccact tcgccgcgtt
    15241 ccagatcatc cagcgtcaac ctgcccgcct tgaagccctg cacatcatac atctccgaag
    15301 cggcgtgcac cggtaccacc tcatcccagt agcgccggtt gacctgcatg cgttcacggt
    15361 cgattttact caccatacgg catcctcccg ggttagcccc gggaggattg taccattggc
    15421 tggtgatggt taggatggat tgatggtgat ggtgcggctg gtcttgatca cttccttcgc
    15481 cttgggcagc gtcacggtca gcacaccatt tttaaagacc gcgctgactt tgtcctcgtc
    15541 cagctgcgtg ttgaacggaa tgacgcgctc aaaagagcca taactgcgct ccacgcggtg
    15601 ataggtgttt tccttttcct ccaccgcgct ctttttctcg ccgcacagtt tcaacacatc
    15661 tttctccagt ttgatgtgga tgtccttctc atccatgccc ggcagctcag cgctcacttt
    15721 gaattcggtc tcggtttcgc tgatatccat tcgcgggaag aattcagcag agcggcgggt
    15781 caaaaactgg ccagggaagg aaaacgcgtc tgagaagaac tcatcgaaca tgcggttcat
    15841 ctcgttctgg atggccagca tgggggtctc tccctcacgg ttaaacagcg gccggagggc
    15901 gagccgattg cccctgaagg ggcgatggac aggaaattct gaattgctca tactttgcct
    15961 cctttttgag tagagaatgg ttatattctc attatgcccc gcgagggtta gaaaaaggta
    16021 tgagaaatat tagaattcaa taagcataat aaaaatgacc tccaactggc tgaggggggc
    16081 aaccagctga aggtcgttta ccagtatagc agatttttta cgtgtttttc ttaataaatt
    16141 tgttcaactg agatcaattt cccaagatca cccggccatc cagcgccagc gcgccctgcc
    16201 cctcggggaa caccagcagc gggttgacct cggcttcctg gatctcaggg tgatcgagcg
    16261 ccaggcggct gaggcgcagc agcacgtcca ccaccgcgtc caggtcggcg cgcggctgcc
    16321 ccctgaagcc ggtgagcagc ctgccggcgc gcgtttcagt gatcatttgc cgcgcgtctg
    16381 cggcgctgag cggcgccacc ctgaaggaca catccttgaa aagctccaca taaataccgc
    16441 ccagcccgaa catcatcagc ggtccaaagc ccgggtcgcg ccgcatgccg atgatgacct
    16501 cctgcccttt gggcgccatg cgttccacca tcacgccttc cagcagcgcg tcagggcgcg
    16561 ccgcgttaat ctccgtcatc atcgcactgt acgcctggcg cagcgctgct tcatccgcca
    16621 ggttgagctt gatgccgccc gcgtccgatt tgtgcagcag ctgcggcgag acgatcttca
    16681 tcaccaccgg gtagccgatg cgcgcagcga ccgcggcggc ttcggctgaa ttcgccgcga
    16741 aaccgccttc caccagcgga atgccgtacg ccgcaagcag tgggcgcgtt tcaacctccc
    16801 ccaggctggc tttgccgccc gccgctttca acacctcccg cccggctttg cgctcaatgc
    16861 cggcgagcgc cggcggcggc acaacagggt tcgcaagcca atccttgtaa tccagcatcg
    16921 ccttcagcac cgcgcccgcg gattctggct gggcgtacat gggaatgcgg tgctcgtgca
    16981 gcaccaggcg cgcttcgtct acagaaatat cgcccatcac gcacgccagc acggtcttct
    17041 cgcgccccgc cgaagcatcc gccaccgcct gcgccacccc cgcggggctc accagcgcct
    17101 gcggtaccag cagcggcagc acaatatcca cctgctcatc cttcattaaa gtgcttgtac
    17161 atagcgcgta atcaggcggt tccgctccgc ccagtatgtc caccgggttg ctcacctgcg
    17221 ccgcggggtt gagcgccgcg cgcaataccg cgcgtgtttt ctctccaagt tcgtcgagca
    17281 ccagcccgtt ggctgagagc gcgtcggatg ccagcgccgc cggaccgccg gcgttggtga
    17341 caattaccgc gcgcctgccc gcgggcagcg gctgaaaatc aaacgccagc gcggtttcaa
    17401 acagcgcgcc gatgctctca acctccacca cgcccgcctg cctgaacgcc gcctggtatg
    17461 ccgcgtaaga ccccgccagc gagccggtgt gcgagctcac cgcccgcgcc ccggcgctgg
    17521 agcgccctcc ttttagcagc acgatcggct tcacgcgcga aacacgccgc gccgtttcta
    17581 aaaagcggcg cccgtctttg atctgttcca cgtaggtggc gatgacccgg gtgcgagggt
    17641 cttcgcccag gtattccatc aggtcggtct cggtcacgtc cgcttcgttg cccatgctga
    17701 tgaaatagga aaaaccgatc ttcttgccca gcacctggtc gaccacgcca cccagtaccg
    17761 cgcccgattg cgccataaac ccaatgccgc cggcatccgg cacgccatga atgaaagtgg
    17821 tgttgagccc gttgtgcagg ttcatcagtc ccacgcagtt ggggccgatc atgcgcatgc
    17881 cataacgatg aatgatctcc aggcagcgcg cttctaacgc ctgaccgccc tcgcccacct
    17941 ctttgaagcc gccagaaatg acaatgaccg ccttcacccc gcgcctgccg caggcttcca
    18001 gcgtctccgc gatgaccggc gccggcagcg caattaccgc cagttccagc gggtctggca
    18061 cggcgctgat atcgctgaag ccgggcttat ccaatatctt ttccgcgccg gggttcaccg
    18121 ggtagatgcc gcccgcgtaa gtggaactca ccaggttgcg catgatgccg aaactgagct
    18181 tgcgcgggtt ggctgaagcg ccgatcaccg ccacaccggc gggttcaaaa aaaggtttaa
    18241 gagaagaggt catggcgtgt ccttatgagg tgttgaggtg ggcggcgggc gcgccgcata
    18301 aagggtgatg ccttaccgac ctgtctggtt aa
//
LOCUS       DAOTLW010000035        18282 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_3458_length_18282_cov_10.269819, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000035 DAOTLW010000000
VERSION     DAOTLW010000035.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 18282)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 18282)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18282
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3458_length_18282_cov_10.269819"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="P5516_06510"
     CDS             <1..410
                     /locus_tag="P5516_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92126.1"
                     /translation="EGEMVESIPVKKNKTWLYILIAVVIIGLLVTGIVLLATAGNTTT
                     TMVRDIFIILMALESIVIGVALVILVIQLSSLINLLQNEIKPILQNTSDTVNTLKGTT
                     TFLSDNLATPVIKLNSSVAGLRKLLNLIGLFRK"
     gene            438..764
                     /locus_tag="P5516_06515"
     CDS             438..764
                     /locus_tag="P5516_06515"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRT92127.1"
                     /translation="MEERDNFGAFLVGFLIGGITGAVVSLLYAPKSGDETRTVIKEKA
                     IELKDKTAVTAEEAYRKAEQAANEAAAKAQELLKTAQSKAAEVLKKAPVVLEEEKVVV
                     KAKKAA"
     gene            complement(811..2406)
                     /locus_tag="P5516_06520"
     CDS             complement(811..2406)
                     /locus_tag="P5516_06520"
                     /inference="COORDINATES: protein motif:HMM:TIGR00196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052855 - ADP-dependent NAD(P)H-hydrate
                     dehydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HRT92128.1"
                     /translation="MKLISVAAMRDLEQRANAAGFTFMAMMQTAGERIALYVDQKHRR
                     GVQPYALGLIGGGNNGGDTLIALSLLQSRGWNTRAILARDRKPHDVLLQEYCRSGGAV
                     VDAAKMNELQHLEGIVLDGIYGTGFRSPLPEPIQNLLSDVSESLPGFAWIAVDCPSGM
                     DCESGEVSRGTVKAAETVCLEAVKRGMLTYSAFPYLGELITVDLGIARSLGRAGEPGD
                     MVITDELVHALLPKREAFSHKGSYGKALVIGGSANYPGAPVLAGRGAYAVGTGLVQVA
                     VPASVYPTGVTSCPELTWVLLEDAGGCISEIAAETVILYWMGCDSAVLGPGLGRHDST
                     RRFVFNLLLRENLAQGEVAGFHGVARRQKTKYRDSGRPPLVIDADGLTLLGEDSAWFE
                     KSNLTAVLTPHPGEMAHLTGLSVEEVQSDRMEVARRFALQWGQTVVLKGPLTVVAGTE
                     GHIAVLPVATSSLAKAGTGDVLAGMIAGFLAQGLSTWEAALVGAWMHAQAGLATRKIV
                     GCDESVLAGDVIRAIPEVYKLIE"
     gene            2475..4031
                     /locus_tag="P5516_06525"
     CDS             2475..4031
                     /locus_tag="P5516_06525"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HRT92129.1"
                     /translation="MRRYVYVVVFISGLASLAVEMSASRLLGNYFGTSNLVWASIIGL
                     ILIYLTAGYFIGGAWADRSPRYLTFFGVLAWSSLAIALIPLASRPILRLASTAFDNLQ
                     IGVLAGSFTAVMILFIIPVSLLGMASPFAIRLSIQDKENAGKISGRIYAISTLGSFIG
                     TFLPVLVLIPLIGTYRTFLVISTVLMTFSLIGIYLDGRWKWLVRYAWMPLVLIALFLW
                     GTPGSDKSTQGLIFEGESAYNYIQVLDEDDFRLLRLNEGQGVHSIYHPTILNFNGPWE
                     QVMSAPFFNPAPVEPASIKSAAIIGLAAGTTSRELFAVFPHVKIDGVEIDPLIVDVGK
                     RYFHMENPNLNVIVQDGRWALERSAEKYQVVSIDAYRPPYIPWHMTTVEFFQSVRAHL
                     AEDGVMIINIGRGPTDRRLVDALAATIRQVFPSIYISDLSGSFNTLLFATVQETSLEN
                     YLANYVHLMENPNTPPLLLEVLAATYEGLQPLPEDEGLVFTDDHAPVEQITNSIVLNF
                     LFSGGTKNLE"
     gene            4028..4693
                     /locus_tag="P5516_06530"
     CDS             4028..4693
                     /locus_tag="P5516_06530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HRT92130.1"
                     /translation="MKVLIRFISILISVVLPFFLLMCSIRMLLNPFFLDYEYNLPNFP
                     ADDFGFTTPDRLKWGKISLLYLTNNESDDYLGTLKLDDGSPLFNQRELSHMLDVRVLV
                     KAALKAWYGVGLFLFLSLALFWRTNNIKRYWQAVSTGGWLTLGLIVLMIAGVFIDFYQ
                     LFAGFHAIFFEGDTWLFFTNDTLIRLFPEKLWSDAFIYLGVFTLISALVCACAGRRLA
                     QRK"
     gene            complement(4722..5468)
                     /gene="gpmA"
                     /locus_tag="P5516_06535"
     CDS             complement(4722..5468)
                     /gene="gpmA"
                     /locus_tag="P5516_06535"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HRT92131.1"
                     /translation="MYKIVLLRHGESTWNKENRFTGWTDVDLTEKGEEEARTAGVLMK
                     AAGLEFDLAFTSVLTRAVRTLWIALQTMDMVWIPILNSWRLNERHYGALQGLNKAETA
                     EKYGDEQVLIWRRSYDTQPPKLTKDDERYPGNDRRYANLTPEELPLSECLKDTVARFL
                     PYWHGEIVPVIKSGKRVLIVAHGNSLRALVKYLDHVSDEEIVGFNIPTGVPLVYELDD
                     ELKPIRSYYLGDPEEVKRKAEAVANQAKKK"
     gene            complement(5547..6662)
                     /gene="ugpC"
                     /locus_tag="P5516_06540"
     CDS             complement(5547..6662)
                     /gene="ugpC"
                     /locus_tag="P5516_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HRT92132.1"
                     /translation="MASVTYENVVKKFGDFTAIENMNLEIHDEEFLVLVGPSGCGKTT
                     ALRCLAGLEEVTSGKIYIGDRLVNDVPPKDRDIAMVFQSYALYPHMSVFDNMAFGLKL
                     RKLPKQEIKQRVEKSAKILGIENLLKRKPRELSGGQRQRVALGRAIVREPKVFLLDEP
                     LSNLDAKLRVQTRAEISKIHNKLRTTFVYVTHDQTEAMTMASRIAVMNKGRLQQIDTP
                     QTLYDQPANMFVAGFIGTPAMNFFTMRLIKDGDSLLVDNGEFKIKIPEDRNKLLMHLV
                     DKEVVFGIRPDDIHNPDYSPLNIHGERVKAKVDVVELMGNEILVYLVSDKGNNFVGRV
                     DPRSKYVIGETAEVVFNMDNSHIFDPSQDPDNPVAVR"
     gene            6814..8628
                     /locus_tag="P5516_06545"
     CDS             6814..8628
                     /locus_tag="P5516_06545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92133.1"
                     /translation="MPPSSHMLDLDLKSTISHHRLAGIWKMMKGFRLRYIGAVTSLGL
                     SALAKTFTFLLLRYFVDTYLIQQDNRIGLPYIALGFVGLAILEGGFTFLSGVLSSQTA
                     EGITRRLRNYLYDHIQHLSFTYHSKTKTGELIERSTSDVDALRRFFSDQATNIGRILM
                     LFIINFVTLLNLNVKLAWVSIIVIPFILAVSIFLFKKVSSAYESYQQQEAILSTTLQE
                     NLSGVRVVKAFSRQRYEMDKFEKDNWEKYTRGKKLLIMHSLFWPVSDILCGAQLIAGY
                     LIGAMMAINGEISVGTYLAYSGLVIYIIYPLRNLGRIIVQTSTGLVSYSRVMDIIKEK
                     REPLDQGDYTPQSNLRGDFAFLNVGFEYEPGDQTLADINFSVKPGQVIALLGSTGSGK
                     STLVHLLPRFYEYTSGKILLDGVELNRYPRKFLRQQIGIVEQEPFLFSRTIRENITYG
                     VGREVSDEEVEQAARAAAIHDVILSFQEGYQTIVGERGVTLSGGQKQRVTIARTLLKN
                     PRILILDDSTSSVDTETEAEIREALWNLMRNRTTFIIAHRIQSVADADLILVMDKGRI
                     IQHGRHADLINQTGVYREIFNIQARIEDELEKELASAG"
     gene            8618..10441
                     /locus_tag="P5516_06550"
     CDS             8618..10441
                     /locus_tag="P5516_06550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92134.1"
                     /translation="MPDNYFDEEEFETRFNGKTLSRILKLTVPHIKWVIGFLLTIAIV
                     AGLDSIGTYLVKMVIDNGILAGDRTAVIKVLIMYGVLIFVQSGSTFLFIYLAGILGER
                     IRYDLRKKMFNHLQALSLNYYSKTPVGWIMARVTSDSERVADLVTWGLVDSTWGLMSI
                     VTSMVFMVAINWKLALIVLALLPVLFIIATYFQRWILASYRAVRKTNSKIVGAFNENI
                     TGVKVIKALGREPENLDEFKAITEKMYHSSFRAAWLSALFLPTVQIISALAVAAVVWY
                     GGGQAKIGALTVGGIQAFVSYITFMMWPIQDLARVFAEMQQAIASAERMFSLIDSEPQ
                     IQDRPKAIDPGTIKGDIIFDHVTFRYEKDEKPVLNDFSLHVRRGETIALVGPTGGGKS
                     TIINLLCRFYEPESGRIYIGGRDYTTLSLQSIQSRIGVVLQTPHLFSGSITENLRYGR
                     LDASMEEIISATKVIGAYDFIMHFEKGFDTEVGEGGGLLSVGQKQLLSLARAVLANPE
                     VFVMDEATSSVDTLTEALIQKGMQALLDTTTSFIIAHRLSTIKKADRILVIEDGRIAE
                     MGTHTELLRIHGKYHQLYTQQFRSQLETAYNPFQQAATASD"
     gene            10465..11337
                     /locus_tag="P5516_06555"
     CDS             10465..11337
                     /locus_tag="P5516_06555"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HRT92135.1"
                     /translation="MPKRKTKTRTSVKALKGVLTLVFRTLKGAFTTFILAKGAEAAAS
                     ISYYTLFSIFPLLLSILAIGSFIIDQATVERELLNFLPNLLPVSQDFIMTNVQQVFKL
                     RGAISSVAVVGLIWSATAVFTTLIRNINAAWPAAAPHSFIRMRLWSLAIIAAMVLLLI
                     LSSFSLTLRHLLNNLGIPINYSLLSEFLSSFFYKKVLPVLVEVLVFFSLYYWVPQIKV
                     KKMGALTGAVVATFSWQLITAIFSAYLASGLARYEIVYGSLGKMIALLAWIYFSSWII
                     LFCAHLTSSIDRHS"
     gene            complement(11340..12011)
                     /locus_tag="P5516_06560"
     CDS             complement(11340..12011)
                     /locus_tag="P5516_06560"
                     /inference="COORDINATES: protein motif:HMM:NF012257.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HRT92136.1"
                     /translation="MKNLTGYLNDPIFRVLTEKEREQVVASAVRRPVKKGEYLVLQGD
                     IWEYLFFIEQGSVVAEKGSFDGRTFVAATFKPGDLFWGLGFFLDNAVMPANLRVFEDG
                     VIIQWAKRDLKPLLESNGRFSWELCKLVMSKIQYVSEVLETMTFQPIAVRLARLLMSF
                     SDKEERVPIERSLTLDDMAARIGSTREMVCRLLYKFSDEGLIRITRTEFTITDPENLE
                     KRAQK"
     gene            12145..12537
                     /locus_tag="P5516_06565"
     CDS             12145..12537
                     /locus_tag="P5516_06565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92137.1"
                     /translation="MKLKNLWLLILLVLLLSGCASKNPCPEGSVTYLESADEFPPDTS
                     SSLPPTKTDIEIRGKTITVDRVIEGPLCNDTWEGTVYVACDLTVQKWDIKPFFLSNGC
                     NLEIKPGTVVYVAAHNNAAYYKGCTTCH"
     gene            12603..13388
                     /locus_tag="P5516_06570"
     CDS             12603..13388
                     /locus_tag="P5516_06570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92138.1"
                     /translation="MKTKFKLGGILAIIAAIIGIIGHYALFFQFYQAGMHAEAAEPGC
                     EILLEWVHPLLANMGLLAAALFIVSAYGFFTQRRWAFSLSVFGLILTLLGSFFVNIPF
                     MAAGLPPVYFALFFPYTIIYFLMMVLVQRLSWSRTLLALLTGICYILCWMNGIASTSR
                     IITIGADLFTLVQKLHFVAMVGMAVVTAGILLKPREWQRVLGLSAGVTELIVGIPLGI
                     VTTQELGRFSLFSLAPILSLVLVIIFVWPGVWKKWTGGEEKAE"
     gene            13572..15116
                     /locus_tag="P5516_06575"
     CDS             13572..15116
                     /locus_tag="P5516_06575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92139.1"
                     /translation="MKTLYTTLALLIVAVFVLSACAPAAPTAAPVVPVETQAPEAAAA
                     TEAVVATEAVTPEQEEVSLWDQPYISQVPPILMIEPYYTIFGQSQVAVPYYYEEAVKL
                     AGHSCGATAGAWIITKKALEALYPEGETPVRGQIAVEAPGAENEFPVGVLGEIITFIT
                     GAAPKTGFIGQEFGEANNLFKRQNKMVYTDEPSGTPPPKMEWIFTRLDTGKKVGVNFN
                     LAIITPIATEERQAMGAKLAKGGATAEEAADYAVYWNDRAKFVFENADMDGFFNVTVY
                     EEGATETPPSIVSDVKTVAAEDFSWDQAYITEVPPIMMMEPYFQIFGQTQEPVPYYYE
                     EAVKLAGHSCGATTGAWTITRKALEALYPDGQIPVRGQIAVQAPGAEDEFPVGVFGDI
                     ITYVTGAAGKTGFIGQEFGKANDLFVRQNKMVYMDKPSGTLPPKMEWIFTRLDTGAKV
                     GVRFNLVVITPLPTPNRIAMGKKLALGEATPEQAADYYQYWNDRATYVLKNAEMDGFF
                     TVTVYE"
     gene            complement(15262..15924)
                     /gene="phoU"
                     /locus_tag="P5516_06580"
     CDS             complement(15262..15924)
                     /gene="phoU"
                     /locus_tag="P5516_06580"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HRT92140.1"
                     /translation="MRATFEAELKYLKDELLLLGNLVEQQIQGAVESFKQRDPQLSRT
                     IVEKDDEVNEKRYMIEEQVLITIATQQPMARDLRLLASILEIAGELERIGDYAKGIAT
                     INIRMGEEALLTPLVKIPMMAEKCTSMLHRAIQAFIQEDIEEANQIPREDDEVDRLYE
                     EVYSSMMEKIVHDENAIQRANWILWVAHNLERVADRVTNICERTIFTVTGDMKEIRSS
                     DQ"
     gene            complement(15955..16725)
                     /gene="pstB"
                     /locus_tag="P5516_06585"
     CDS             complement(15955..16725)
                     /gene="pstB"
                     /locus_tag="P5516_06585"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003101281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HRT92141.1"
                     /translation="MNTAKIETHNLEVFYGSFKAITDVNVKIPEKRITAFIGPSGCGK
                     STLLRSFNRMNELVPNTRISGEVIFDGRNLYHPDVDPVEVRYRIGMVFQRPNPFPKSI
                     FENVAWAARLHGVKGSLDELVESALRRAALWNEVKDILKQSALSLSGGQQQRLCIARA
                     LAVQPEVILMDEPASALDPIATLKIEELMQSLKESYTIVIVTHNMQQAARASDYTAMM
                     MLKEGTRSGTVIEFNETNHIFTRPADKRTEDYVTGRFG"
     gene            16972..18087
                     /locus_tag="P5516_06590"
     CDS             16972..18087
                     /locus_tag="P5516_06590"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF002872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent 6-phosphofructokinase"
                     /protein_id="HRT92142.1"
                     /translation="MEIARTPIRKVAISTGGGDAPGLNAVIRAAVLAGLERGWEMYGI
                     RDGYNGLLMPEKYLEGGVVNLTRDRVRGITHLGGTIIGTTNRGNPFSYPVKNLDGTYT
                     EVDRSDELVRAFNMHEFDALISVGGDGSMSIANSLSQKGLRVVGVPKTIDNDLDETVV
                     TFGFDTAVSFAVECLDRLHSTAASHRRILVVEVMGRYAGWIALESGIAGSADVVLIPE
                     IPYDINLVVKKIIDREKAGRNFSMVVVAEGAIPKGGTVTVQSKEVGRAERLGGVGEKV
                     TEALQELTGKEARLVVLGHLLRGGSPTANDRLIALRFGAAAVRALDEGQEGVMVALDP
                     PTVKYVPLSDATRRMKSVPLDCDTMLTARDLGMCFGD"
BASE COUNT         4305 a         4779 c         4436 g         4762 t
ORIGIN      
        1 atgaagggga aatggttgaa agcatcccgg tcaagaaaaa taagacctgg ctttatattc
       61 tcatcgcggt ggtcatcata gggcttctcg ttaccggaat cgttctgctg gctacagctg
      121 gtaataccac tactaccatg gtaagggata ttttcatcat cctcatggcg cttgaatcca
      181 tcgtgattgg tgtggctttg gtcatcctgg tgattcaact ttcctcgctc atcaatctgc
      241 tccaaaatga gattaaaccg atcttacaga acaccagtga tacagtgaat actttgaaag
      301 gaaccaccac ttttttaagt gacaacctgg ccacaccggt aattaagctc aatagttctg
      361 tcgcaggatt gagaaaatta ctcaatctca tcggtctatt tagaaaataa cgattcttaa
      421 taaaaaggag atatgacatg gaagaaagag ataattttgg cgcgttcctg gttggttttc
      481 tgattggtgg aatcacgggt gccgtggtga gcctgcttta tgccccaaaa tctggtgatg
      541 agacgcgcac cgtgatcaaa gaaaaagcga tcgaattaaa agataagacc gccgtgaccg
      601 cagaagaagc ttatcgcaaa gccgagcagg ctgccaatga agccgccgcg aaagcgcagg
      661 aactgttgaa gaccgcccag tcaaaagcag cggaagtgct caagaaagcc ccggtggttt
      721 tggaagaaga aaaagtcgta gtaaaagcca aaaaagcagc ctgaagctga atatgttatc
      781 aaaacaacct ccctcacagg aggttgtttt ctattctata agtttataca cttctgggat
      841 tgctcttatc acatcacccg ccaataccga ttcatcacaa cccacaattt tcctggtagc
      901 cagtccggcc tgcgcgtgca tccaggctcc aaccaaagct gcctcccacg tagaaagacc
      961 ctgagcgaga aaaccagcga tcattcccgc caacacatct cctgtgcccg ccttggcaag
     1021 gctggaagtc gccacaggca ataccgcaat gtgaccttct gttccggcaa ccacagtcaa
     1081 agggccttta agcacgactg tctgccccca ttgcagggca aatcgccggg ctacttccat
     1141 tcggtcgctt tggacttcct caaccgaaag ccctgtgagg tgcgccattt ctccggggtg
     1201 tggggtgagc accgctgtga gattgctttt ttcaaaccac gcggagtctt ctcccagcag
     1261 ggtaagcccg tcagcgtcta ttaccaaagg aggtcgccca gaatctctgt atttcgtttt
     1321 ttgtcggcgt gcaactccat gaaacccggc aacctcacct tgagccagat tttcacgcag
     1381 gagcaggtta aacacaaaac gcctggtaga atcatgcctg cctaaccctg gaccaaggac
     1441 cgcactatcg cagcccatcc aataaaggat gactgtttca gccgcgattt ccgaaatgca
     1501 acctccagcg tcctcaagaa gcacccaggt cagttccggg caagaggtca ctcccgttgg
     1561 ataaaccgaa gctggtacgg cgacctgcac cagaccggtt cccacagcat acgctccgcg
     1621 ccctgcaagt actggtgcgc caggataatt cgcagatccg ccgatcacaa gcgccttacc
     1681 gtaactcccc ttatgagaaa atgcttctct ttttggcagg agggcgtgaa caagctcatc
     1741 ggtaatgacc atatctccag gttcacctgc cctgccaaga gaacgagcaa tacctaggtc
     1801 aaccgtgata agctctccaa gataagggaa ggctgaataa gtgagcatcc ctcgcttaac
     1861 cgcttcaagg cacacggttt cggctgcctt taccgtgccg cggctcacct cgccgctctc
     1921 acaatccatc cctgatgggc aatccacagc gatccaggca aaccccggca aggattcaga
     1981 aacatcactt aagagatttt ggattggctc aggtaatggg cttctgaagc ctgtgccata
     2041 aatcccatcc agcactatgc cttcaaggtg ctgcaattca ttcattttcg ccgcatcaac
     2101 tactgcccca ccgctccgac aatattcctg taatagaaca tcatgtggct ttcgatctcg
     2161 ggcgagaatc gcgcgcgtat tccagcctct tgattgcaaa agcgaaagcg caattagtgt
     2221 atctccgcca ttatttcctc cccctattaa ccccaatgcg taaggttgaa cgcctcggcg
     2281 atgtttttgg tcaacataaa gtgcgattct ctcacccgct gtttgcatca tcgccataaa
     2341 tgtgaatcca gccgcgtttg cacgctgttc caaatcacgc atagccgcga cactgatcaa
     2401 tttcatctca gctccctgaa cacaattatt atacaacgag ggttgcctac ctcatctcaa
     2461 gtataatgat gcgcatgcgg cgttatgttt atgtggtggt ttttatttct ggcctggctt
     2521 cgttggctgt ggaaatgtcc gcgtcaagac tgctcgggaa ttatttcggc accagcaacc
     2581 ttgtctgggc aagcatcatc gggctgatcc tcatttacct cacagcgggt tatttcattg
     2641 gcggcgcctg ggcggatcgc tctcctcgtt acctgacctt ttttggcgtc ctcgcctggt
     2701 cttcattagc aatcgcgctt atcccccttg cctctcgccc aattctccgc ctggcttcca
     2761 ctgcatttga caacctgcaa attggcgtgc tggctggatc ttttaccgcg gtgatgattt
     2821 tattcattat ccccgtcagc ctgctgggta tggcatcgcc ctttgccatc cgtctttcca
     2881 tccaagacaa agagaacgcc ggcaagattt ccggccgcat ttacgcgatc tcaacattag
     2941 gctcgtttat cggaacattt ctacccgtgt tggttctgat ccctcttatc ggcacttaca
     3001 gaactttcct ggttatctca acggtcttaa tgactttttc tctcatcggt atctacctgg
     3061 atggcaggtg gaaatggctg gtgcgttacg cctggatgcc cctggtgctc atcgcgttat
     3121 tcctttgggg tacgcctggg tcagacaaat caacccaggg cctcattttc gaaggagaat
     3181 ctgcttacaa ttacatccag gtgctcgatg aagacgattt ccgtttgctg cgtctgaatg
     3241 aaggacaggg cgttcattct atttaccatc caacaattct caattttaac ggcccctggg
     3301 aacaggtaat gagcgctcct tttttcaatc ccgcgccggt tgagcccgca tctattaaaa
     3361 gcgccgccat cattgggttg gctgccggta ccaccagccg tgaattgttc gcggtttttc
     3421 cacacgtgaa aattgacggc gttgagatag accctctcat tgtcgatgtg ggaaagcgct
     3481 actttcatat ggagaacccc aacctgaatg tgattgtcca agacggtcgt tgggcgctgg
     3541 aaaggagcgc cgaaaaatac caggtggtca gtatcgatgc ctaccgccct ccctacatcc
     3601 cctggcacat gaccactgtg gagttttttc aatccgttcg cgcacacctc gctgaggatg
     3661 gcgtgatgat catcaatatc ggtcgcggac caacggacag gcggttggta gacgctctgg
     3721 ctgcaaccat tcgccaggta ttcccctcca tctatatttc cgatctctca ggttctttca
     3781 acaccctcct ttttgccaca gtgcaggaga ccagcctgga aaactacctg gcaaattatg
     3841 ttcacttaat ggagaatcca aacactccac cgcttttatt ggaagtgttg gcagcaacat
     3901 acgaaggctt gcagcccttg ccagaagacg agggtttggt cttcacagat gaccacgctc
     3961 ctgttgagca gattaccaat tctatcgtgc ttaacttttt gttttcagga gggacaaaaa
     4021 acctcgaatg aaagtgctga tcagatttat ctctatatta atttctgttg tcctgccatt
     4081 tttcttattg atgtgctcca tccggatgct gctcaaccct tttttccttg attatgagta
     4141 taacctgcca aattttcccg ccgacgattt tggattcacc acacctgacc gcctgaagtg
     4201 ggggaaaatt tccctcttgt acctcacaaa taatgaaagc gatgattatc ttggtacgtt
     4261 aaaactggat gatggttcgc ccttgttcaa tcagcgcgag ttgagccaca tgttagatgt
     4321 ccgtgttttg gtgaaagcag ctttgaaggc ctggtatggc gtggggctat tcctcttcct
     4381 gtcgttggca cttttttggc gaacaaacaa tattaagcgg tactggcaag cggtatccac
     4441 cggtggctgg ttgactctcg ggcttatcgt gttaatgatc gccggggtgt tcatcgattt
     4501 ctatcaactt ttcgccggtt tccacgccat cttcttcgag ggcgacacct ggttgttctt
     4561 caccaacgac acgctgatcc gtcttttccc tgaaaagctt tggtccgacg cattcatcta
     4621 tttgggggtg ttcactctca tcagcgcgct ggtgtgcgct tgcgcaggcc gaaggctggc
     4681 tcaaagaaaa taataaccgg cagttccctg ccggtttgtg ttcatttttt ctttgcctgg
     4741 ttggccacgg cttcagcttt ccgcttgacc tcctcggggt cgcccaggta ataactcctg
     4801 attggtttca gctcatcatc cagctcataa accaacggta caccggtggg tatgttaaac
     4861 ccaacgatct cttcatccga aacatgatcg aggtatttca ccagcgctct caggctgttg
     4921 ccatgcgcta cgatgagcac gcgttttccc gattttatga caggtacaat ctcaccatgc
     4981 cagtatggaa gaaaacgggc aacagtatct ttcaggcatt cgctcaacgg gagttcctca
     5041 ggcgtcaggt tcgcgtagcg tcgatcgttt ccagggtagc gctcatcgtc tttcgtgagt
     5101 tttggcggct gggtgtcata acttctgcgc cagatcagca cctgttcatc gccatacttc
     5161 tccgccgtct cggctttatt cagaccttga agggcgccat aatgccgctc attgagacgc
     5221 catgagttaa ggatgggaat ccacaccata tccatggtct gcagcgcgat ccacagtgtg
     5281 cgtaccgccc gtgtcaacac agaagtgaaa gccaggtcga attctaatcc tgccgccttc
     5341 atcaaaacgc ccgccgtgcg cgcttcttcc tcgccttttt cagtcaggtc aacatccgtc
     5401 cagccagtga agcgattttc cttattccag gtactttctc catgccgaag caaaacaatc
     5461 ttgtacatcg cttatcctct ttcttgttta tttaaaaagt agcgcaaggt tggtacttgc
     5521 gctacatcta tttactcaaa atcacattat cgtacagcca cgggattatc tgggtcctgt
     5581 gaaggatcaa agatgtggct gttatccatg ttgaagacca cttcggctgt ttcaccaatg
     5641 acatacttgg agcgagggtc aactctgcca acaaaattat ttcccttgtc agataccagg
     5701 tagaccagga tctcgtttcc catcaattcc accacgtcaa ccttggcttt aacacgctca
     5761 ccatgaatgt tcaagggtga ataatcgggg ttatgaatat cgtctggccg gataccgaat
     5821 accacttcct tatcaaccag gtgcatcaag agcttattcc gatcctcagg gattttaatc
     5881 ttgaattctc cgttatccac gagtagagaa tcaccatcct taatcaggcg catcgtaaag
     5941 aagttcatcg ctggggtgcc aatgaacccg gcaacaaaca tattcgcggg ttgatcataa
     6001 agcgtttgcg gtgtgtcgat ttgctgcagc ctgcccttgt tcatcaccgc gatgcggctc
     6061 gccatcgtca tggcttcggt ctgatcatgg gttacgtaca caaaagtggt gcgcagtttg
     6121 ttgtgaatct tgctgatttc cgcgcgggtt tgtaccctca gtttggcgtc caagttagaa
     6181 agcggttcat ccaggaggaa aaccttcggt tcgcgcacaa ttgccctgcc gagcgccaca
     6241 cgctgtcgtt gcccgccaga aagttcacgc ggtttgcgtt ttaataggtt ttcaattccg
     6301 agtattttgg cggatttttc cacgcgttgc ttgatttcct gtttgggcag tttgcgtaat
     6361 ttaagcccaa acgccatatt gtcgaacaca gacatgtgag ggtaaagcgc gtaggactga
     6421 aataccatgg cgatgtctcg gtcttttggc ggcacatcat taaccagccg gtcaccgata
     6481 tagatcttcc cagaagtcac ctcttccagc ccagccaggc aacgcagcgc ggtggttttt
     6541 ccacagccgg aaggaccgac cagaaccaga aactcttcat catgaatttc caggttcatg
     6601 ttttcgatgg cggtaaaatc gccgaacttt ttcacaacat tttcatacgt aacgcttgcc
     6661 atgctttcct ccttcttgaa cgagtatagt gactgtatta tataacaaaa tgaaagctga
     6721 ctcttttctt attctcgctt tcatatatcc ttattgatta ggtcagttga atgtgcttta
     6781 taattcaagt tcaccatcgc tctgagggac atcttgccac catcttcgca catgcttgac
     6841 ctggacttaa aatccaccat ttctcatcat cgcctggcgg gtatctggaa aatgatgaaa
     6901 gggttccgac tgcgctatat tggcgcagtt accagcctgg ggctatccgc cctggcgaag
     6961 acctttacat tcctgctgct gcgctatttc gttgatacct atttgatcca gcaagacaac
     7021 cgcattggct tgccgtatat cgccctgggg tttgttgggc tggcgatact cgaaggcgga
     7081 tttacgtttc tcagcggggt cctgtcttct cagaccgcgg aaggtattac ccggcgcctt
     7141 agaaactacc tctatgatca catccagcac ctctctttta cctatcattc caaaaccaaa
     7201 accggtgaat tgattgagcg ctccacttca gatgtagacg ccttacgccg tttcttttcg
     7261 gaccaggcaa ctaatattgg tcgtattttg atgctcttca tcatcaactt tgtcaccctg
     7321 ttgaatctca atgtgaaact cgcctgggtc tcgatcattg tcattccatt tattctggcg
     7381 gtgtccatct ttcttttcaa aaaggtgtcc agcgcttatg aatcttacca acagcaggaa
     7441 gccatccttt ccaccacact ccaggagaat ctctcaggtg ttcgggtggt gaaagccttt
     7501 tcgcgccagc gttatgaaat ggataagttt gaaaaagaca attgggagaa atacacccgc
     7561 gggaaaaaat tactgatcat gcattcgctc ttctggccgg tatctgacat tctgtgcggc
     7621 gcgcagctga tcgcgggcta tctcatcggc gcgatgatgg ccatcaacgg tgagatctct
     7681 gtgggaacct atctggccta cagcggtctg gttatctaca ttatctaccc gctgcgcaat
     7741 cttggcagga ttattgtgca aacctccacg gggttggtgt cttatagccg ggtcatggac
     7801 attatcaagg aaaagagaga gcctctcgac cagggagatt acaccccaca atccaacctg
     7861 cggggagatt ttgcgtttct caatgttggg tttgagtacg aacctggaga ccagacgctc
     7921 gcagatatca atttcagtgt taaacccggg caggtgatcg cgttgcttgg ttcaactggc
     7981 tcaggtaaat ccacgctggt acatttgctg cccaggttct atgaatacac ctcagggaaa
     8041 atcttgctgg atggcgtcga attgaaccgc tatccacgta aattcttacg ccaacagatt
     8101 ggcattgtgg agcaggaacc gttcctgttt tctcgcacca tccgtgagaa tatcacctat
     8161 ggtgtagggc gtgaagtcag cgacgaagaa gttgaacagg cggcgcgcgc agccgcaatc
     8221 catgatgtta ttctatcttt ccaggaaggt taccagacca ttgtcgggga gcgcggcgtg
     8281 actctttcag gggggcagaa acagcgtgtt accattgcgc gcacgctgct taaaaacccg
     8341 cgtattttaa ttcttgatga ttccacctct tcggtagaca ctgaaaccga agcggagata
     8401 cgagaagccc tgtggaacct gatgcgaaac cgaaccactt tcatcatcgc ccatcgcatt
     8461 caaagcgtag ccgacgctga cctcattctg gtgatggaca aagggcggat cattcaacac
     8521 ggcaggcacg ctgacctgat caatcaaaca ggggtgtacc gcgaaatatt caacattcag
     8581 gcccgcattg aggatgaact ggagaaggag ctggccagtg ccggataatt atttcgacga
     8641 agaggagttc gagaccaggt tcaacggcaa aacgttaagc cgtatcctca aactcactgt
     8701 tccccatatc aagtgggtga ttggcttttt actgaccatc gccatcgtgg ccggtctgga
     8761 ttctatcggg acctacctgg tcaaaatggt gatcgataat ggcattctgg caggcgaccg
     8821 tacagctgtg ataaaagtgc ttatcatgta cggggtatta attttcgttc aatctggcag
     8881 cacgttcctt ttcatttacc tggcagggat cctcggcgaa cgcatccgtt atgacctgcg
     8941 caagaagatg ttcaatcacc tgcaggctct gtcgctgaat tactacagta aaacaccggt
     9001 gggctggata atggctcgtg tgacatcaga ttctgagcgc gtggcggacc tggtcacctg
     9061 gggactggtc gattctacct gggggctgat gagcatcgtc acatcgatgg tcttcatggt
     9121 tgctatcaac tggaagctgg ctctgatcgt attggcgctg ttaccagtgc tttttatcat
     9181 cgccacctat ttccaacgct ggatcctcgc ttcataccgc gcggtgagaa aaaccaactc
     9241 caagatcgta ggcgccttca atgaaaatat caccggcgtc aaggttatca aagccctggg
     9301 gcgcgaacca gaaaatctcg atgaatttaa agcaatcaca gaaaaaatgt accattcttc
     9361 attccgggca gcgtggctca gcgcgctttt cttgcccact gttcaaatta tttccgcgct
     9421 ggcggtggca gccgtagttt ggtatggcgg cggacaggcg aagattggcg cgctgacggt
     9481 tggcggaatc caggctttcg tttcatacat cacgtttatg atgtggccaa tccaggacct
     9541 ggcgcgtgtg tttgctgaaa tgcagcaggc tatcgcttcg gcagagcgga tgttttcatt
     9601 gatcgattca gaaccgcaaa ttcaagaccg gcctaaggcc atcgacccag gcaccatcaa
     9661 aggcgatatc atcttcgacc atgtgacctt ccgttatgag aaagacgaaa aaccggtatt
     9721 gaacgatttc agcctgcacg tacggcgggg agagacgata gccctggtgg gtcctacagg
     9781 cggtggaaaa tcgaccatca tcaaccttct ttgccgtttc tatgagcccg aatccgggcg
     9841 catctacatt ggaggccgtg attacactac cctttcgctt cagtcaattc aatcccgcat
     9901 cggcgtggtg ctgcaaactc cgcatctttt ttcaggcagt atcactgaaa acctgcgtta
     9961 cggtcgcctg gatgccagca tggaggagat tatctccgcc acaaaagtga tcggcgctta
    10021 tgatttcatc atgcactttg aaaaagggtt tgacactgaa gttggcgagg gtggcggtct
    10081 gctctcagtg gggcagaaac aattgttaag cctggccagg gctgtattgg ccaaccctga
    10141 ggttttcgta atggatgaag cgaccagctc ggtcgacacg cttactgaag cgctgataca
    10201 gaaagggatg caagccctgc tggataccac gaccagcttt atcatcgctc accgtctatc
    10261 taccattaag aaagcagaca ggatcctggt gatcgaagat gggcgcatcg cggaaatggg
    10321 aacccacacg gaacttctca ggattcatgg aaaatatcat caactttata ctcagcagtt
    10381 ccgctcgcag ctcgaaacgg cgtacaatcc cttccagcaa gccgcgacag cgtcagacta
    10441 gcatgattta agggatgaat acttatgcca aagaggaaaa cgaaaacaag aacaagcgta
    10501 aaagcgttga aaggcgtgtt aaccctggta tttagaacct taaaaggggc atttaccacg
    10561 tttatcctgg ctaaaggagc ggaagccgcc gccagcatct cttattacac tttgttttcc
    10621 atctttcctt tgctattgag catcctcgcg ataggctcct ttattattga ccaggcaaca
    10681 gtggaacgtg aactgctgaa ctttttgccc aacctgctgc ctgtatcaca agacttcatt
    10741 atgaccaacg tgcagcaagt attcaaattg cgtggagcga tctcatccgt cgctgtggtt
    10801 gggctgatct ggtccgccac agctgtgttc acgaccctca tccgtaatat caacgccgcc
    10861 tggccggcag ctgcgccgca cagttttatc cgtatgcgcc tgtggtccct ggctatcatc
    10921 gctgcgatgg ttttactgct gattttgtct tctttctcgc tgaccctgcg tcatttgctc
    10981 aataacctgg gaatccctat caactattcg ctattgagtg aattcctttc ctctttcttt
    11041 tacaagaagg tcttgcccgt gctggtagag gtactggtgt ttttcagcct gtattactgg
    11101 gtgccacaga ttaaagttaa gaaaatgggc gcgctgaccg gagcggtggt agccactttt
    11161 tcatggcagc tcatcactgc catattcagc gcgtatctcg ccagtggatt ggcaagatat
    11221 gagattgtat atgggtcact ggggaaaatg atcgccctgc tggcatggat ctattttagc
    11281 agttggatca tcctgttctg cgcccacctg acctcatcca tcgaccggca tagttgaagt
    11341 tacttttggg cgcgtttttc caggttctcg gggtcagtga tggtaaattc tgtccgggtg
    11401 atgcgaatta acccttcatc agaaaatttg tataacaggc ggcagaccat ttctcgggtg
    11461 ctgccaatgc gcgcagccat atcatccagg gtcaggctgc gctcgattgg cacccgctct
    11521 tctttgtctg agaagctcat caacagccgc gccagcctga cggcaatagg ttgaaaggtc
    11581 atcgtttcca atacctcgct cacatactgg atcttgctca tcaccagttt gcataattcc
    11641 caagaaaaac gcccgttact ttcaagcaga ggttttaagt ctcgcttcgc ccactgaata
    11701 atgaccccat cttcgaaaac acgcaggttc gccggcataa ccgcgttatc caggaagaag
    11761 cctaaccccc aaaaaaggtc gccaggttta aaggtagccg cgacaaatgt gcgtccatca
    11821 aatgagcctt tttcagcaac cacggaaccc tgctcaatga aaaacaaata ttcccaaata
    11881 tctccctgca acaccaggta ctcgcctttt ttcaccggtc ttctaaccgc tgaagcgaca
    11941 acctgctcgc gttctttttc ggtcaacacg cggaagattg gatcattcaa ataacctgtc
    12001 aagtttttca taagcaacct ccccggtttt catatcccaa ctaattctat accagtcaca
    12061 acagaaatgc ccgcaattta actaaaagca aagtcatgca acgctgtttt tgtcataatt
    12121 gaagaaacct gactggagaa aaatatgaaa ttaaagaacc tttggctgct catcctattg
    12181 gttttacttt tatccggttg tgcatcaaaa aacccgtgcc ctgaaggtag cgtgacctac
    12241 ctggaaagcg cggacgaatt ccccccggac acttcatctt ccttaccccc taccaaaaca
    12301 gatattgaaa tccgagggaa aaccatcacc gttgatcgcg ttattgaggg tcctttgtgc
    12361 aacgatacct gggaaggaac cgtttatgtt gcctgcgacc tgacagttca aaaatgggat
    12421 attaaacctt tctttctctc taatggctgt aacctagaga taaaaccagg caccgttgtc
    12481 tacgtagccg cgcacaacaa cgctgcttac tacaaaggtt gtacgacctg ccattgattt
    12541 ttttcataga ggttaacaac tcagaacacc aggctgttca tcataatttt taaggagaaa
    12601 acatgaaaac aaaattcaaa ctcgggggaa tcctggccat cattgctgcc atcattggca
    12661 tcatcggcca ctatgcactc tttttccaat tctaccaggc aggcatgcac gcagaagccg
    12721 ctgaaccggg ctgcgagatc ctcctggagt gggttcaccc attactggcg aatatgggat
    12781 tgttagcggc agcgttgttc atcgtcagcg cttacggctt ctttacacag cgccgatggg
    12841 ctttttcgct atcagtgttc gggctcatcc taacgctgct tggcagcttt ttcgtcaata
    12901 tcccattcat ggctgcagga cttccgccag tctattttgc cctctttttc ccatatacga
    12961 tcatctactt tctgatgatg gtactggtgc agcgtttatc gtggagccgc actttgctgg
    13021 ctctgctgac cggcatttgc tacatcttgt gttggatgaa cgggattgcc agcacaagcc
    13081 gcatcatcac aattggtgct gacctgttta cactggttca aaagcttcac tttgtcgcga
    13141 tggttggcat ggctgtggtc acagcaggaa ttttactaaa accgcgtgag tggcagagag
    13201 ttctcgggtt aagcgccggg gtaacagaat tgatcgtggg aatcccgttg ggtatcgtca
    13261 ccacccagga attaggtcgt ttctcgttat tctcgctcgc ccccatcctt tcactggtat
    13321 tggtgatcat ttttgtttgg ccaggcgttt ggaaaaaatg gaccggagga gaagaaaaag
    13381 cggagtagcc gaccctgtcc aaaacatgga gtacatctac caccccattt tcatcctcaa
    13441 aacgactaaa agcgcattga agtcctatct atggtgaagg gtccgcgtga tccttcacca
    13501 tttgtgcgga aagagaatga tgcacacaaa taactttttg ttcgttcaac tcacacacaa
    13561 ggagaagaga aatgaagacg ttgtacacca cattggcatt actgatcgtt gctgtttttg
    13621 tcctttcggc ttgtgcccct gctgccccaa ccgcggctcc ggtggtgccg gtcgaaactc
    13681 aggcgcctga agcagcggct gctacagaag cagttgttgc aaccgaagcg gttacgcctg
    13741 agcaagaaga ggtcagcctt tgggaccagc catacatttc acaggttccg cctatcttga
    13801 tgattgaacc gtattacacc atttttgggc aatcgcaggt ggcggttcct tactactacg
    13861 aagaagcggt caagctggct ggccattctt gcggagccac ggctggcgcg tggatcatta
    13921 cgaagaaagc gcttgaagca ctctatccag aaggagagac tcctgtacgt ggacagattg
    13981 ccgtggaggc cccaggcgct gaaaatgaat tcccggttgg cgtgcttgga gagattatca
    14041 cctttatcac tggagccgcg cccaaaacag ggtttatcgg ccaggaattt ggtgaggcca
    14101 acaacctctt caaacggcag aataagatgg tttacacgga tgagccttcg ggaacccctc
    14161 cgccaaaaat ggaatggatc ttcaccaggc tggacaccgg caaaaaagta ggcgtgaatt
    14221 ttaacctggc gatcatcacc cctatcgcca cagaagagcg ccaggcaatg ggcgcgaagc
    14281 tggccaaagg tggcgctact gcagaagaag ctgcggatta tgctgtgtac tggaatgacc
    14341 gagccaaatt cgttttcgaa aatgcggata tggatggttt cttcaatgtg actgtgtatg
    14401 aagaaggggc cactgaaacc ccgccttcaa ttgtctcaga tgtaaaaaca gtcgctgctg
    14461 aagatttctc ctgggaccag gcatacatca ccgaggttcc tcccattatg atgatggagc
    14521 cctatttcca gatattcggt caaacccagg aacctgtgcc ttattattac gaagaagcag
    14581 taaaattggc cgggcattcc tgcggagcga ccacaggcgc gtggacgatt acacgtaaag
    14641 cccttgaagc gctttatcca gatggccaga tcccggtgcg cgggcagatt gccgtacagg
    14701 ctcctggagc tgaagatgaa ttccccgttg gggttttcgg tgacataatc acctacgtca
    14761 ccggggctgc cggcaaaaca ggatttatcg gccaggaatt cggtaaagcc aacgacctct
    14821 ttgttcgcca gaacaaaatg gtgtatatgg ataaaccctc tggcacgctt ccgccaaaaa
    14881 tggaatggat ctttaccagg ctggataccg gcgcaaaggt gggtgtgagg ttcaatctgg
    14941 tggtgatcac cccactgcca acgcccaatc gcatcgccat gggtaagaag ctggcgcttg
    15001 gtgaagccac cccggaacag gcggctgatt actaccaata ctggaacgac agagccactt
    15061 atgtattgaa aaatgcggag atggatggct tcttcaccgt gacagtctac gaataaccct
    15121 gttagtgcaa tagagagcaa aatagcggct ggtcagccgc tattttgttt ttcctgtttc
    15181 aagtaaaaaa gatgtaaggt taaactcagc cctcgtcttt ggggcgttca tttttcaggc
    15241 catgcgggcc actcaggtct tttattggtc tgaagacctg atctctttca tatcgccggt
    15301 aacggtaaaa atggtgcgtt cgcaaatatt cgtcacccgg tcagcgaccc tctccaggtt
    15361 gtgcgccacc cataagatcc agttggcgcg ctggatggcg ttttcatcat ggacgatctt
    15421 ctccatcatc gagctgtata cctcttcgta taagcggtcg acttcatcgt cttccctggg
    15481 aatttgattc gcctcttcga tatcttcttg gataaacgcc tggattgcgc ggtgcagcat
    15541 actggtgcat ttctcagcca tcatcgggat ttttaccagt ggggtgagca gtgcctcctc
    15601 gcccatgcgg atgttgatgg tcgcgatgcc tttggcgtaa tcaccaatgc gctcgagttc
    15661 cccagcaatt tcaaggatcg acgccagcaa cctcaggtcg cgcgccatgg gctgttgggt
    15721 ggcgatagtg atgagcacct gttcctcgat catataccgt ttttcattaa cctcgtcatc
    15781 cttttcaaca attgttcgcg aaagctgtgg gtccctttgc ttaaacgact cgaccgctcc
    15841 ttggatctgt tgttccacca gattgcccag gagcagaagt tcatccttta aatatttcaa
    15901 ttctgcttcg aatgtcgcgc gcatatttcc ctcgctttat tttcattgta acatttatcc
    15961 gaatcttccg gtcacgtaat cttccgtgcg tttatccgca gggcgcgtga agatatggtt
    16021 ggtctcgtta aattcaatca ctgtgccaga acgcgtgcct tctttcaaca tcatcatcgc
    16081 cgtgtaatct gaagcccgcg ccgcctgctg catattgtga gtgacgataa caatggtgta
    16141 actctcctta agtgactgca tcaattcttc aatcttcaaa gtcgcgattg ggtcgagcgc
    16201 ggacgccggt tcatccatca agatcacttc gggctgcaca gccagcgcgc gcgcgatgca
    16261 caggcgttgc tgctgcccgc cagatagcga aagcgcggat tgtttaagga tgtctttcac
    16321 ttcattccac aacgccgccc gcctgagtgc agactccacc agttcatcca ggctgccttt
    16381 gacgccgtgc aggcgcgccg cccaggctac attttcgaag atggatttcg gaaaggggtt
    16441 cgggcgttga aataccatac caatacggta acgcacttcc accgggtcca catccgggtg
    16501 atatagatta cgcccatcaa agattacctc accactgatc cgagtgttgg gaacaagctc
    16561 gttcatgcgg ttgaacgacc ggagcaatgt ggattttccg cagccggaag gtccgataaa
    16621 agcggtgatg cgtttttcgg gtatcttgac gttcacgtct gtgatggcct taaaacttcc
    16681 gtaaaagacc tcgaggttat gcgtttcgat cttggcggta ttcatcctga tggttttctc
    16741 caattaatcg cccacacctc tctgtacgac cctttacacc ggttcttcac ggcttaaaat
    16801 aagattgtat cacaacggta tcccggaggt ttctttctta cgaaacaccc acagaagtgg
    16861 gtcctccctg tcgccgcggt aaaaaaggat tttcgtttca tctctgcttt ccactgacca
    16921 gatttctttt ataattcaag tattcttata actccaaaat ggaaggttgc catggaaatc
    16981 gcccgtacac ccatacgtaa agttgccata tccaccggcg gcggagacgc cccagggttg
    17041 aacgcggtca tccgcgctgc tgtgctggcg gggttggagc gcggctggga gatgtacggc
    17101 atccgcgatg gttataatgg cttattgatg cccgagaaat accttgaggg cggcgtggtc
    17161 aacctcacgc gagaccgcgt gcgcggtatc acccaccttg gtggaaccat catcggcacc
    17221 accaaccgcg gcaacccgtt cagctacccg gtcaagaacc tggacggcac ttacactgag
    17281 gttgaccgtt cggatgagct ggtacgcgct tttaatatgc acgagtttga tgcgctcatc
    17341 tccgttggcg gtgacggctc catgtctatc gccaattcat tgtcgcaaaa ggggctgcgg
    17401 gtagtgggtg tgcccaagac gattgacaac gatctggacg aaacggtggt cacgtttggt
    17461 tttgataccg cggtcagttt cgctgtagag tgcctggacc gcctgcacag cacagcagcc
    17521 agccatcgtc gtatactggt ggtggaggtg atgggccgct acgcaggctg gatcgcgctg
    17581 gaatccggta ttgctggctc agccgacgtg gtgctcatcc ctgagattcc ctatgatatc
    17641 aaccttgtgg tgaaaaaaat aatcgaccgc gagaaagccg ggcggaattt ctcgatggtg
    17701 gtagtcgccg aaggcgccat tcccaaaggc ggaacggtca ctgtgcaatc aaaggaggtt
    17761 gggcgcgcgg agcgcctggg cggcgtggga gagaaggtca ctgaagcgct gcaggagctc
    17821 accggtaagg aagcccgcct ggtggtgttg ggtcacctgc tgcgcggcgg ttcgcccact
    17881 gccaatgacc ggcttattgc gctgcgtttc ggcgcggcag cggtgcgcgc gctggatgag
    17941 gggcaggaag gtgtgatggt ggcgctcgac ccgcccacgg tgaagtacgt gcccctgtca
    18001 gacgcgacca ggcgcatgaa gagtgtaccg ctggactgcg acaccatgct caccgcacgc
    18061 gaccttggta tgtgttttgg ggattgattt tctttttagg tcggggtggg cagcagaaaa
    18121 tccatcgcgg ttgctctttt tacccctttt tcatcgccgt acgtacgtgg agatggtttc
    18181 agtttcccag gctctccgtt aaattagcgc ctgatgaatt ttacgcgcgc atccatgcgg
    18241 gagggtgaag acgtgtaccc tgacggatat ttggatacac ac
//
LOCUS       DAOTLW010000036        17526 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_3636_length_17526_cov_11.042528, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000036 DAOTLW010000000
VERSION     DAOTLW010000036.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 17526)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17526)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17526
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3636_length_17526_cov_11.042528"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..816)
                     /locus_tag="P5516_06595"
     rRNA            complement(<1..816)
                     /locus_tag="P5516_06595"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
     gene            complement(1155..1227)
                     /locus_tag="P5516_06600"
     tRNA            complement(1155..1227)
                     /locus_tag="P5516_06600"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1192..1194),aa:Ala,seq:tgc)
     gene            complement(1247..1320)
                     /locus_tag="P5516_06605"
     tRNA            complement(1247..1320)
                     /locus_tag="P5516_06605"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1284..1286),aa:Ile,seq:gat)
     gene            complement(1451..2951)
                     /locus_tag="P5516_06610"
     rRNA            complement(1451..2951)
                     /locus_tag="P5516_06610"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            3516..5390
                     /locus_tag="P5516_06615"
     CDS             3516..5390
                     /locus_tag="P5516_06615"
                     /inference="COORDINATES: protein motif:HMM:NF016202.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HRT92143.1"
                     /translation="MTRTRAHLSPSLWLQLGTSLWIGLAATLALLLISHILPLLVFGN
                     RIFPGVSLSGLNLSGLNANEAAALISRSAYYPASGRITLEYGSQQWALSPSQLGVMLD
                     PQATAITAYKIGRSGPLEKVLLDRFNLLRGSAEVQPTFIFNEKATVDFLNAIAAEINQ
                     PLQEASLEIQGVDVFVRNGQPGRVLDVAASLARIAAQVEQVRDGVVPLAVIETQPLIS
                     DVSEQAELAREILSQPLRFTLPAEVGEIEGNWMLEPTALAKMLTFERVQDGGTDSLAV
                     VVNPTLAGLHLDSLRESLDAEPQNARFIFNDETGLLDLKDSAVIGRKLNIQSSLENIN
                     SSLKAGSHEAQLVFDITPPAVTDSMTGAELGITELVYEYTSYFYGSSPDRVQNIKTAS
                     SSFHGLLVPPGGVLSMADELGDISLDNGYAEALIILGDETIKGVGGGVCQVSTTLFRT
                     AYFAGYPILERHAHAYRVGYYEQTPSGGHNANLAGLDATVFVPLVDFKFKNDSDHWLL
                     METYVYNNTALTWKFYSTKDGRQVEHSTTGPTQKVKAPDDLYRENPELKKGEVKQVDW
                     AAEGARVVVTRRVTRGGELLYNDTFTTVYEPWRNIFEYGPGTEDMPPDKKSGSAEDEE
                     "
     gene            5441..5665
                     /locus_tag="P5516_06620"
     CDS             5441..5665
                     /locus_tag="P5516_06620"
                     /inference="COORDINATES: protein motif:HMM:NF015899.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HRT92144.1"
                     /translation="MKVNQELLEILRCPACVKEKPGLLEQVREVWLVCTDCGRKYPIV
                     EEIPVLLISEGERWRALPVEDLPVPPPRPE"
     gene            5763..7205
                     /locus_tag="P5516_06625"
     CDS             5763..7205
                     /locus_tag="P5516_06625"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRT92145.1"
                     /translation="MTKKPMAHSIRRNLKMDTTTRVLLISFAVVGLLLAFLAGKFIYN
                     TVKSWSITSLPGAPVSSPGSSATTGQIPDLNIQSNTGPEAKSWDGKSRVNILFLGLDA
                     TDQRELMEPGPRFSDTMILVTIDPLSQTLGALSIRRDLWVNVPGYDYHKINKAHFIGE
                     AYHLPGGGAGLAVATVEEFLGIPIHYYAKVDFNTFVRVIDEIKGVKIEVKEPILADWG
                     ANGNNFWLEPGVYTLPGTYALAYARYRGSDAGDIDRGERQMQVMMAIRDRILDFKMLP
                     QLIQSAPALYQDVSDGVQTNMSFDQAVQLMVLISKIPRENFKTYNITYDDAAPEMITT
                     LDEGTQYILRPFPDKIRELRDRVFASEGVAAAPIVMQSGGDPLALAQAEGARISIFNG
                     TSTNGLAEATRDYLTSQGLHVIEASSASEAYSYTTLVVHSATPYTLAYLSSLMNNIPS
                     TRIFNQYDPQSTTDITVFLGTDWATANPMP"
     gene            complement(7259..8095)
                     /locus_tag="P5516_06630"
     CDS             complement(7259..8095)
                     /locus_tag="P5516_06630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92146.1"
                     /translation="MKEKRGSLYLLTGLVIGAVLGLLTSIFIVPARYVDVAPSALKAV
                     DREQYRALVARAYLVEADNGRAVARLALLADPRPADALVAQAEKEKRPGGDEHTSRAL
                     ALLAAAVSQKQLLITPLPRIAAEAGGQATEPAPGEQETPTLPPPTRTPGATITPRPSP
                     TPLPTQGAPYALIQQKEICEVEGRGSLIQVFVFNAADEGVPAVRVEVSITDGGVESFF
                     TGFYPEISPGYADYAMVEGMTYNLRVGSTGQLVQNLSVPQCKNKDGEAFPGSIQLVFK
                     QP"
     gene            complement(8092..8445)
                     /locus_tag="P5516_06635"
     CDS             complement(8092..8445)
                     /locus_tag="P5516_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92147.1"
                     /translation="MRKGRLIAFILAIMLGATAGLMYGWLYYPAKISAAAMKSLRADY
                     KADYVLMVAELFATDGDLEKATDRLEKIKPADAIGAVRRGLLTAQEMGYADWEMRLIA
                     DLEIALQQTRQETRP"
     gene            complement(8446..10107)
                     /gene="hutU"
                     /locus_tag="P5516_06640"
     CDS             complement(8446..10107)
                     /gene="hutU"
                     /locus_tag="P5516_06640"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF003820.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HRT92148.1"
                     /translation="MVETRVIRAPRGSTLTCKNWQIEAAYRMIQNNLDPEVAERPQDL
                     VVYGGRGQAARNWESFDAILRSLRELEPDETLLVQSGKPVVVFKSHADAPRVLIANSN
                     LVPHWATQQHFDQLAAKGLIMFGQMTAGSWIYIGTQGILQGTYETLGSLARQKGWQSL
                     RGRFVLTAGLGGMGGAQPLAITMNEGVGLCVEVDPQRAQRRLETGYVDVVVDSLEEAM
                     TLVEEFCQREEPRSIALIGNAAEIYPELVLRGVVPDLVTDQTPAHDVMSYIPAGLSLE
                     EAERMRSDDPRQYEHRSLDSMSAHIRAMLHFQRKGAEVFDYGNNLRQRAFDNGVKDAF
                     QFPGFVPAYIRPLFCEGKGPFRWVALSGDPEDIYRTDEAISKLFPRDEHLKRWLTMAR
                     ERVHFQGLPSRICWLGYGERARAGLLFNEMVARGQLKAPIVIGRDHLDAGSVASPNRE
                     TEGMQDGSDAISDWPILNALVNAAGGATWVSFHHGGGVGIGFSQHAGMVIVADGTPQA
                     AKRLERVLTTDPGMGVVRHADAGYPEAIMAARRLGIHMPMLPKDQ"
     gene            complement(10215..10487)
                     /locus_tag="P5516_06645"
     CDS             complement(10215..10487)
                     /locus_tag="P5516_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92149.1"
                     /translation="MDRRGVLGRRCGWPWTDWYRNAQSQPRVEIQVGEHCTRATFKAL
                     EEPGRVAEFLAYRLKKRPLLTRLVLRQGGLRGQISPPVLLAYARQI"
     gene            complement(10640..11968)
                     /locus_tag="P5516_06650"
     CDS             complement(10640..11968)
                     /locus_tag="P5516_06650"
                     /inference="COORDINATES: protein motif:HMM:NF024116.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92150.1"
                     /translation="MRFIDLVSLILYNLGRRKGRVLLTAVGVVIGTAAVVVLVSLAVG
                     LQKSATQQLWGISDLTSIEVYPGYQESRVMGGGGGGGVMYAGGGSDMKLLTPAAIKEI
                     SAIPGVRKTIIHDYLQSGSEVHFGKLMSWGGIIGVNIDDLADMGLKAARGTTELSRGT
                     MVIGSWVPQNFYNPNPRPGEEMTGTPELLDQVIRLVLVKYTQDGEEVRRTFNVRVVGI
                     LEETRGMADGNMYMRLDEVTAFSEWGRGTRINRDKEGYGMLIVKAEDPDVVLDIADQI
                     NALGYMANTPQSYLEGINSFFLVLQIIFGGVGAIALLVAAIGIANTMAMAILERTREI
                     GLMKAVGATNRDVLSIFLGEAAGIGFVGGLGGVALGWGASAILNVVALSYFASQAANG
                     GGSLPTAATSTPFWLPVFALVFATVIGLLSGLYPALRAASLVPVNALKYE"
     gene            complement(11978..13741)
                     /locus_tag="P5516_06655"
     CDS             complement(11978..13741)
                     /locus_tag="P5516_06655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92151.1"
                     /translation="MIKKNRNVLGLLLTLTFLFGMTTLNTYAAPLNDEVPVDSKSKSG
                     LPGSVITYKLDIPGEAGQIMNVSANSTSGWNSPTVVPNTITFGANETKTVSVNVPIPV
                     GATEGQSDITTVDVTVVAGNPVEFDPLQLTTTVVVPKPAIGRPMVTITSYSTGNKTLF
                     AGNEMNLQVVLGNNGKVSAENIIVTFDGTDFYPRESGGVRTTGTLPPGNTNTITQKFL
                     IGDALAWANVASMKATVNYTDPLGTAYSEVFTLSFVIAEPSSSGFAATATPNIPQRPQ
                     LVVTGYSTDIDPLQPGSIFELKLDVKNLGMADAKNVSAVIGGGGMPSNEEGKPQPGGV
                     SGGSSDLTTFAPLGSSNVIFMGDVSKDQTAVVTQKLVVNVTAQPGAYPLKLSFVYIDA
                     KGNRLVDDQVITLLVYSLPQVEVSFYRDAGIITAGMENILPLQVTNLGKKTYVLGNMK
                     VSAENADLLNNILLVGALDPGGYYTLDASLTPYQEGPLDLKVVINYTDDFNQPRFIEQ
                     VIPVEVQPAMEVPPDMGDGNTPNGEGPVVPAPETFWQKVVRFFKGLFGLDSGLQQPVE
                     TYPVQETPSGEVIPVPAIPKG"
     gene            complement(13738..14481)
                     /locus_tag="P5516_06660"
     CDS             complement(13738..14481)
                     /locus_tag="P5516_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92152.1"
                     /translation="MLDKALVRTRDLTKRYTMGENTVVALDGVSLEIDPGSFTVVMGP
                     SGSGKSTLLYLLGGLDWPTSGSITIGEDEIEKMDENLLAEYRRNRVGFIFQSFNLIPS
                     MPAEENVSFPLRFARIPRAQRHARAQEILQKVGLSDRATHKPTELSGGQQQRVAVARA
                     LINDPPLILADEPTGNLDSQSGFTIMKLLSRLHQAGKTVIVVTHDPRMRQFATNMLYL
                     LDGKVVSEEAYRKATENMTQPIVEQEEIL"
     gene            complement(14499..15278)
                     /locus_tag="P5516_06665"
     CDS             complement(14499..15278)
                     /locus_tag="P5516_06665"
                     /inference="COORDINATES: protein motif:HMM:NF016764.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HRT92153.1"
                     /translation="MALNAKGQQTNKHSWNTKWIPSLFTRPARTTSEIVTQEKAAWLT
                     PLLILSVLVILAVAIGAPIKRNVIQMGSELPPDFQYYTPEMQEQFFAAQAKQTSPLFL
                     YVFPLISGLLKVWMPWFLLSILMYLSLTLAGSRAGSIKSYNLVAWSMLPMAVRLLIQI
                     LAMIVSKTVVSAPGLSGFIAAEATGVGAYLRSMLGFIDIYFLFQIALLLLGSVPLSGL
                     SRSKAWTATAISVVIVLLLMAIPGLLASALGGLSLRGGFYF"
     gene            15393..16100
                     /locus_tag="P5516_06670"
     CDS             15393..16100
                     /locus_tag="P5516_06670"
                     /inference="COORDINATES: protein motif:HMM:NF024273.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HRT92154.1"
                     /translation="MRINIGVENNYEGRSLAWMLDYPGCFAYGAEGSEAILRAPQALL
                     RYKEWVDSHLKDSWMKSLGDFDIHLEESYDVFSINDDFDLADEGIEVNAWFRHDWKPL
                     TAEDIRRGTLVLTWSREDLLELVSGLTPAQLDCRFSGERWSIAGVLEHIANAEHWYLH
                     RLGLAVGTRQELPADVFERLKVVRRRLVEVLPSLQGSRQVLGVDGEFWSPRKVLRRSA
                     WHEVDHIEHIFKLISRL"
     gene            complement(16427..17521)
                     /gene="amrS"
                     /locus_tag="P5516_06675"
     CDS             complement(16427..17521)
                     /gene="amrS"
                     /locus_tag="P5516_06675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HRT92155.1"
                     /translation="MERQMNPHELLDVLTQPAELVETLEQGSLRCLACAHRCLLKPGR
                     RGICGVRFNANGVLMAPKGYVAGVQIDPLEKKPFNHFLPGSEVLTFGMLGCNFHCGFC
                     QNWLSSQALRDPEAGRSAYAIRRVSAEKLVTYAVENGARAIASSYNEPLITTEWAVEV
                     FKLAKDKGLKTAYVSNGYATAEVLDYLVPWLDGYKVDLKSMRDSEYRKMGGRLQPVLD
                     TIRRIKELGLWLEVVTLVIPGMNDSSDELWEAGRFLASISPDIPWHVTAYHPDYKENA
                     PPTPAETLQRAAEIGQEAGLRFVYAGNLPGRVGSLENTICPQCGRAVIERRGYLLHGY
                     HINSAGACAFCGQPIAGVWSGRPQAGHANG"
BASE COUNT         3825 a         5180 c         4523 g         3998 t
ORIGIN      
        1 ttcgggcttc cacgagcgat tagactcgct tcaccctgtc catgggtagc tcactcggtt
       61 tcgggtctaa tccttgcgac tcagcgccct attcagactc gctttcgcta cggcttcgag
      121 tgtcactctc ttaacctcgc cacaaagatt aactcgcagg ctcattctcc aagaggcacg
      181 ctgtcaggcg tagcggcgcg cacccggctt tcaccggagc accgacccgc tattagccct
      241 ccaactgctt gtaagtttac ggtttcaggt tctatttcac tcccctcaac ggggtacttt
      301 tcacctttcc ctcacggtac ttgttcacta tcggtcgcca agtgtattta gccttggagg
      361 gtggaccccc cagattccta ccgtattagc gtgcacggta gtactcaggt acctgacgga
      421 aggtgttggg ttttcgttta cgggactgtc accctctctg gttggctttc ccacaccatt
      481 ctactaacca aacactttgt aacttcacaa tgtcaggccc tacaacccca ctacggcaag
      541 ccgcagtggt ttgggctttt cccctttcgc tcgccactac tcagggaata ctctcttttc
      601 ctccgggtac ttagatgttt cagttccccg ggtgcccttc gatccgccta tatattcagc
      661 ggatgatgcc agcggttcgc gctggcgggt ttccccattc ggaaatctcc ggatatatcg
      721 cctgtttacg gctccccgaa gcttatcgca gtaacctacg tccttcatcg gcacttggcg
      781 ccaaggcatc caccataaac tcttagtaac ttctcacaca tgatgcggag aactcgatac
      841 tctttatcgt atcatttatt acagattaca ttcgatcaaa actttatgtt gttttgactt
      901 ggcctacata ctattctata aggttggtaa ggtgctgctg actcatcagg gtggtcagtc
      961 gccggttgaa gggcgatcag tgtcactcac gtacacgtca ggggcactga acccacttca
     1021 acctattcat ttttcaaaga gacaagtcga cccggctcac cgccgggtct tatcaaccgt
     1081 gcggatctgc cattttttat atttcggtcg cgcttcttgc cactcactgc tccaattatt
     1141 tttttgcagc tcagtggaga tgaggggatt cgaacccctg acctccgccg tgcaaaggcg
     1201 gcgctctccc aactaagcta catccccgat gtttccgggt ttcaggtggg cctttcagga
     1261 ctcgaacctg agacctcgcc cttatcagag gcgcgctcta accaactgag ctaaaagccc
     1321 aagatcagtc ccttagaaac tgagaagtga caggaaaatc ttgccttgcg tcttcttttg
     1381 cgtctcgaca ccgcgccgag acaattgaga gctagaacat cccccgaagg ttcagttcaa
     1441 gactccatag aaaggaggtg atccaggcgc accttccggt acacctacct tgttacgact
     1501 tcgtcccagt catcaacccc accctcgacg gctccctcct tgcggttagg ataccggctt
     1561 caggtgttgc caactcccat gacgtgacgg gcggtgtgta caagccccgg gaacgtattc
     1621 accgcagtat agctgacctg cggttactag caactccatc ttcatgcagg cgggtttcag
     1681 cctgcaatct gaactgagac cagctttatg gattggctcc acctcgcggt ttggcaacct
     1741 attgtgctgg ccattgtagc gtgtgtgtag cccaggatat aaaggccatg ctgacttgac
     1801 gtcatcccca ccttcctccg gcttaagacc ggcagtcccg tgtgacacat ataacacaca
     1861 gcgagggttg cgctcgttag cggacttaac cgaacatctc acgacacgag ctgacgacag
     1921 ccatgcagca cctgtgcaag ctccccgaaa ggtcggtcac ttttcagttt cctaccactt
     1981 gcatgtcaaa ccctggtaag gttcttcgtg tagcatcgaa ttaaaccaca cgctccgctg
     2041 cttgtgcggg gccccgtcaa ttcctttgag ttttaacctt gcggccgtag tccccaggcg
     2101 gtgaacttat cgcgttagct tcagtaccga tggatttaac cccaccgaca ctaagttcac
     2161 atcgtttaca gctaagacta ccggggtctc taatcccgtt tgctacctta gctttcgtgt
     2221 ctgagcgtca gaaatgggcc aggagaccgc tttcgccact ggtgttcctc cgcatatcta
     2281 cgcatttcac cactacacgc ggaattccat ctccctctac cactctctag tctgatagtt
     2341 ttgaacgacc tctcccggtt aagccaggag ctttcacatc caacttatca aaccgcctgc
     2401 acacgcttta cgcccagtaa atccggataa cgctcgcctc ctacgtttta ccgcggctgc
     2461 tggcacgtag ttagccgagg cttattctaa aggtactgtc cttcctcatc cctttaaaaa
     2521 gcgctttaca acccgaaggc cttcatcgcg cacgcggcgt tgctggttcc cggtttccca
     2581 gattaccaat attccctact gctgccaccc gtaggtgtat ggaccgtgtt tcagttccat
     2641 tgtggggggc caccctctca ggtcccctac ccgtcatcgc cttggtaggc tattacccta
     2701 ccaactagct gatgggacgc aggcccctcc cgaagcgaat aaatccgttt agtcgtaagg
     2761 catctaatcc aaacgaccac atggggtatt agcaatcctt tcggactgtt atccccctct
     2821 tcggggcagg tcaccaacgc gttactcacc cgttcgccac taagacagta ttgctacagt
     2881 ctccgttcga cttgcatgta ttaagcacgc cgccagcgtt catcctgagc caggatcaaa
     2941 ctctccgcca aataacacct ttcggtgtaa gttacggatc tggtttgtta ctcttttgaa
     3001 aacgacaggg catcgttttc ctatcactct tcagttgtta aggtactgat ttccaagggc
     3061 cagatttttc tctcgcgacc gccgctcgtt aagaccaggt gacaaaatta ccgatgctcc
     3121 tttacaggac gtcatcggcc tgcctttacg atttgcttgt ggacaagtcc tcaagcttgc
     3181 tgcgtttgtt gggttgttac ggatctgctt attccagcga aatcttcaac gcacttggga
     3241 cagatattgt aaccgcctgt cttttccctg tcaagggtgt gttcaacgag tgggtcactt
     3301 ctcgagtctg gttattgggt tttcaatgat ctgctcttta ggcgagaccg tttgccgtgt
     3361 tatgggcaac gagggatgat tatacctgct gatgtgtttg tgtcaagggt taatcagcac
     3421 caatttcacc tctttttcaa cttccaaggg gaacttttct ttccttttgt gcgtttgaag
     3481 aatagggcga aacaggagtg ttataatctg aattcatgac caggacacgc gcgcaccttt
     3541 caccttccct gtggctgcag ctcggcacct ccttatggat tggtcttgcc gcgaccctgg
     3601 cgttgctgct catttcccac attctcccct tactggtctt tggcaaccgt attttccctg
     3661 gcgtttcact ttccggtttg aacctttcag ggctgaacgc caacgaagcc gccgcactca
     3721 tcagccgttc agcatactac cctgcatcag ggcgcatcac gctggaatac ggttcgcaac
     3781 aatgggcgct tagcccttcc caactggggg tgatgctcga cccccaggct acagccatca
     3841 ccgcttataa aatcgggcgc agcggccctc tggaaaaagt gctgctggac cgttttaacc
     3901 tccttcgtgg cagcgcggag gtacagccaa ccttcatctt caacgaaaaa gccaccgttg
     3961 acttccttaa cgctatcgcc gcggaaatca accagccact gcaggaagcc agccttgaaa
     4021 ttcaaggggt tgacgtcttt gtgcgcaacg gtcaacccgg gcgcgtactc gatgttgccg
     4081 ccagcctggc gcgcatcgcc gcccaggtcg aacaggtgcg cgatggcgtt gtacctctgg
     4141 cggtgatcga gacccagccg cttatctcag atgtcagcga gcaggctgaa ctcgcccgcg
     4201 aaatcctcag ccagccattg cgctttaccc tgccggcgga ggtcggtgag atagaaggaa
     4261 actggatgct tgagccgacc gcgctggcaa agatgctcac ctttgagcgc gtacaggatg
     4321 gcggtactga ttcactcgcc gtggtcgtca accctaccct ggcagggttg cacctggatt
     4381 ccctgcgcga gtcgcttgac gctgaaccgc aaaacgcgcg ctttatcttc aacgatgaaa
     4441 ccggtctgtt agacctgaag gattcggcgg taatcgggcg caagctcaac attcagtcat
     4501 cgctggagaa tatcaatagc agcctcaaag ccgggagcca tgaagcccag ctcgtctttg
     4561 atatcacccc gcccgctgtc accgattcaa tgaccggagc ggagctgggg atcaccgagc
     4621 tggtttacga atatacgtct tacttctatg gttcctctcc agaccgcgtg caaaatatca
     4681 aaaccgcctc ttcctcattc catggtctgc tggtgccgcc cggaggtgtc ctttccatgg
     4741 ctgatgaatt gggtgatatc agcctggata acggttacgc tgaagccctc atcatcctgg
     4801 gtgatgaaac catcaaaggc gtaggcggcg gtgtttgcca ggtgagcacc accctgttcc
     4861 gcacagctta cttcgccggg taccctatcc tagaacgcca cgcgcacgcc taccgcgtgg
     4921 ggtattacga acagaccccc agtggtgggc ataacgccaa cctcgccggt cttgatgcca
     4981 cggtttttgt gccgctggta gattttaaat ttaagaacga cagcgaccac tggctgttga
     5041 tggaaactta cgtgtacaac aacaccgcgc tcacctggaa gttctactcg accaaagacg
     5101 gccgccaggt ggagcacagc actaccggac ctacccaaaa agtgaaagcc cccgatgacc
     5161 tttatcgtga aaaccctgaa ctaaaaaaag gcgaggtcaa acaggtggat tgggccgcgg
     5221 aaggcgccag ggtggtggta acccgccggg tcacacgcgg cggcgagctg ctgtataacg
     5281 acacattcac cacggtgtat gagccctggc gcaacatctt tgaatacggc ccgggcactg
     5341 aggacatgcc ccccgacaag aaaagcggca gcgcggaaga cgaagaataa ccccccagta
     5401 taatggtaca attgccccgt aatgaaaaag cggaggatga atgaaggtca accaagaact
     5461 gcttgagatc ctgcgctgcc cggcctgcgt taaagaaaaa cccggtttgc tcgaacaggt
     5521 acgcgaggtg tggctggtat gcacagactg cggccgtaaa tacccgatag tagaagagat
     5581 accggtgctg ttgatttcgg aaggcgagcg ctggcgcgcc ctgccagtgg aggacctgcc
     5641 cgttcccccg ccaaggcctg aatgattttt tctcccagct ccaattatct ggtattgttc
     5701 ttgcaacaaa gcaccggtaa cacactaacc tcattaatga tggtgaactc ttaatggctc
     5761 aaatgacaaa aaaacccatg gcgcactcaa ttcgccgcaa cctcaaaatg gataccacca
     5821 cccgggtgtt attgatctca ttcgccgtgg tcggcctgct gctggcattt ttagcgggta
     5881 aattcattta taacacggta aaaagctggt cgatcacctc tctgcctggc gcgccggtta
     5941 gttctcctgg cagcagcgca accacagggc agattcctga tttgaatatc caatccaaca
     6001 ccgggccaga agccaaatca tgggacggta aaagccgcgt gaatattctg ttcctcgggc
     6061 tggacgccac cgaccagcgc gagctgatgg agccgggacc tcgtttttca gacaccatga
     6121 tcctggtaac catcgacccg ctctcgcaaa ccctcggcgc gctctccatc cgccgtgacc
     6181 tgtgggtgaa tgtcccgggt tacgactatc acaagatcaa caaggcgcac tttatcggtg
     6241 aagcctatca cctccccggc ggcggcgccg gtctggctgt tgccacagtc gaggaatttc
     6301 tcggtatccc catccattat tacgccaaag tggatttcaa caccttcgtc agggtgatcg
     6361 atgaaattaa gggggtaaag atagaggtca aggaacccat cctggcagat tggggcgcga
     6421 acggcaataa cttctggttg gaacccggtg tgtacaccct gcccggcacc tacgcgctgg
     6481 cctacgcgcg ctaccgcggc agtgatgccg gcgatattga ccgcggtgaa cgccagatgc
     6541 aggtgatgat ggccattcgc gaccgtatcc tcgatttcaa gatgcttccg cagctcatcc
     6601 aaagcgcgcc cgccctatac caggatgttt ctgatggcgt gcaaaccaac atgtccttcg
     6661 atcaggcagt gcagctgatg gtattgataa gtaaaatccc gcgcgagaat tttaagacct
     6721 acaacatcac ttacgatgac gccgcgccag agatgatcac caccctggac gaaggcaccc
     6781 agtacatcct gcgtcccttc cctgataaga tacgcgagct gcgagaccgg gttttcgcct
     6841 cagaaggcgt ggcagccgct cctatcgtca tgcagagtgg tggagaccca ctggcgttgg
     6901 cccaggctga aggcgcccgt atcagcatct tcaacggtac cagcaccaac ggcttggcag
     6961 aagccactag ggattatctt acttcacagg gcttgcacgt tattgaagcg tcctctgcca
     7021 gtgaggctta ttcttatacc acgctggtgg tacacagcgc cacaccatac acactggctt
     7081 acctgtcttc gctgatgaac aatattccca gcacgcgcat ttttaaccag tacgaccctc
     7141 aaagcacgac ggatatcacc gttttcctgg ggaccgattg ggctaccgcc aacccgatgc
     7201 cgtaaacgca gcggttgttg cgcaatctca tcaggccggg caaccggcct gatttatttt
     7261 atggttgctt aaaaacaagt tgaatggaac ccgggaacgc ttctccatcc ttgttcttgc
     7321 attgtggaac agaaaggttt tgcaccaact gccccgtgct gccaacccgc aggttatagg
     7381 tcatcccctc caccatcgca taatcagcat agcccgggct gatttcaggg tagaacccgg
     7441 tgaaaaaact ttcaacgccg ccatcggtga tggaaacctc cacgcgcacc gccggtaccc
     7501 cttcatctgc tgcgttaaac acaaaaacct ggatcaacga cccgcgtccc tccacttcgc
     7561 aaatctcctt ttgttgtatc aacgcgtaag gagcgccctg tgtgggcaag ggggtggggc
     7621 tggggcgtgg ggtgatcgtg gcgccgggtg tgcgggttgg agggggaagc gtgggcgtct
     7681 cctgctcacc aggggctggt tcggttgcct gcccgccagc ttctgcggca atgcgcggca
     7741 atggcgtgat cagcagctgt ttttggctta ccgccgcggc cagcagcgcc aacgcccggc
     7801 tggtatgctc atctccgccg ggtctcttct ctttttcagc ctgcgccacc agggcatccg
     7861 ccggcctggg gtccgccagc agcgccagcc gtgcaacggc gcgaccatta tctgcttcca
     7921 ccaggtacgc ccgggcgacg agcgcgcggt actgttcacg atcgaccgcc ttcaacgccg
     7981 atggggctac atccacatat cgagcaggaa caataaaaat agatgtgagt aaacccagga
     8041 cggcgccaat gacaagcccg gtgagcaggt acaaggaccc gcgcttttcc ttcatggcct
     8101 ggtctcctgc ctggtttgtt gcagggcgat ttccaggtcg gcaatcaggc gcatttccca
     8161 gtcggcgtag cccatttcct gggcggtcag caatccgcgc cgaaccgctc caatcgcgtc
     8221 agccggttta atcttctcca gacggtcggt agccttctcc aggtctccat cggtggcgaa
     8281 caattcggct accatcagca cgtaatcagc tttatagtct gcccgtaaac ttttcatcgc
     8341 cgcagccgat atttttgccg ggtaatacaa ccaaccatac atcagcccgg cggttgcgcc
     8401 aagcataatg gcgagaatga aggcgatcaa cctgcctttg cgcatctact gatccttcgg
     8461 cagcatgggc atgtgaatgc ccaaccgcct ggcggccata atggcctcgg ggtatccggc
     8521 gtcagcgtgc ctgaccaccc ccatgcccgg gtcggtagtc aacacgcgct ccaggcgttt
     8581 ggcagcctgc ggggtgccat ccgccacaat gaccatgcca gcgtgctggc tgaaaccgat
     8641 tcctaccccg ccgccatgat ggaatgaaac ccaggtcgcc ccgcccgcgg cgttgaccag
     8701 cgcgttgagg atcggccagt cactgatggc atccgagccg tcttgcatcc cctctgtttc
     8761 gcggttaggg gaggcgaccg agccagcatc caaatggtcg cgcccaatga caatgggcgc
     8821 ttttaattga cctctggcaa ccatttcatt aaataacagt cctgctcgcg ctcgctcacc
     8881 ataccccagc cagcagatgc gcgagggcag cccctggaaa tgcacccgct ctcgcgccat
     8941 ggttaaccag cgcttgagat gctcgtcacg cggaaacaac ttactgatgg cttcgtccgt
     9001 gcggtaaata tcttcggggt cacctgaaag cgccacccac ctgaagggac cttttccctc
     9061 gcaaaacaac gggcggatat acgccggcac aaaaccgggg aattgaaaag cgtcttttac
     9121 accgttatca aaggcgcgct ggcgcaggtt gttgccataa tcgaacacct cagcgccctt
     9181 gcgttggaaa tgcagcatgg cgcggatgtg cgccgacatc gaatccagtg agcgatgctc
     9241 gtattgccgc gggtcatcac tgcgcatcct ctctgcctct tctagtgaaa gccccgccgg
     9301 gatgtaactc attacatcat gcgccggcgt ctggtcggtc accaaatctg gcaccacgcc
     9361 gcgcagcacc agttcggggt aaatttcagc ggcattaccg atgagcgcga tggaacgggg
     9421 ttcttctcgc tggcaaaatt cctccaccag ggtcatggcc tcttccagcg aatccaccac
     9481 cacatccaca taacctgttt ccaggcggcg ttgcgctcgc tgggggtcaa cctcaacgca
     9541 cagtcccaca ccttcgttca tggtgatagc cagtggctgc gcgccgccca tgcctcccag
     9601 accggcggtc agcacaaacc gaccgcgtaa tgattgccag cccttttgcc tggccagcga
     9661 tcccagggtt tcgtaagtcc cctgcaaaat cccctgggtg ccaatataaa tccacgagcc
     9721 cgctgtcatc tgcccgaaca tgatgagccc ttttgccgcc agttggtcaa aatgctgctg
     9781 cgtggcccaa tgcggaacca ggttggaatt ggcgataagc actcgcggcg cgtcagcgtg
     9841 gcttttaaaa acaaccaccg gcttgcccga ttgcaccagc agtgtttcat ccggttcgag
     9901 ttcacgcaaa gatcgcagga tggcatcaaa ggattcccag ttgcgcgccg cctgcccgcg
     9961 cccaccatac accaccaggt cttgcgggcg ttccgccacc tcggggtcaa ggttattttg
    10021 gatcatgcgg tatgcggctt cgatctgcca gttcttacag gtgagcgtac tcccgcgggg
    10081 agccctgatc acgcgtgttt ccaccatgcg tcctccgttc ttttccggcc tgttttttcg
    10141 attattatac gctgaaatac aaacccctta cgccgcacgc gttacgggct gctatcaggg
    10201 atgataaccg cacctcaaat ctgcctggcg taagcgagca gcaccggcgg tgatatttgc
    10261 ccgcgcaacc cgccctggcg cagaaccagc cgcgtaagaa gcgggcgctt ttttaagcga
    10321 tacgccagga attccgccac gcggccaggt tcttccagcg ctttaaaagt ggcacgggtg
    10381 caatgctcac ccacctgtat ctccaccctt ggctgggatt gcgcgttgcg gtaccaatca
    10441 gtccacggcc atccgcagcg ccttcccagt acgccccgtc gatccattca tactgcagtc
    10501 caactgtgtg aggtttgccg ctccgcctgc cagtggtggt aagcagcaag accatctcgc
    10561 cgacgagtcg atacaaaaaa atgcgataaa gccgggtgaa tttatggttg tctggcctga
    10621 accttatgta acgggccgtt cactcgtact tgagcgcgtt aaccggcacc aaagatgcag
    10681 cgcgcagcgc cgggtacaac cctgaaagca gcccgatgac ggtagcaaaa accagcgcaa
    10741 aaacaggcag ccagaagggc gttgatgtgg cggctgtagg cagagagccg cccccgttgg
    10801 cagcctggct ggcaaaatac gaaagcgcca ccacattgag gatagcgctg gctccccagc
    10861 ccagggccac gccacccagg ccgcccacaa aaccaatgcc ggcggcttcg cccaggaaaa
    10921 tgctcagcac gtcgcgattg gtggctccca ccgctttcat cagtccaatc tcgcgcgtgc
    10981 gctcgaggat ggccatggcc atggtgttgg caataccaat cgccgccacc agcagcgcga
    11041 tggcgccgac gcctccgaag atgatttgca gcaccaggaa gaaagaattg ataccctcca
    11101 ggtaagattg cggcgtattc gccatgtaac ccagggcatt gatctggtca gcgatatcca
    11161 atactacatc ggggtcttcc gccttgacga tgagcatgcc atagccttct ttatcgcggt
    11221 tgatgcgtgt ccctcgtccc cattcgctaa aagccgtcac ttcatccagg cgcatgtaca
    11281 tattcccgtc cgccatgccg cgcgtttctt caaggatacc cacaacgcgc acattgaacg
    11341 tacgtcgaac ttcttcccca tcctgtgtgt atttcaccag caccaatcga atcacctggt
    11401 cgagcagttc tggggtcccg gtcatctctt cgcccggccg cgggttagga ttgtaaaaat
    11461 tctgcggtac ccaggaccca atgaccatgg tgcccctgga caattcagtg gtgccgcgcg
    11521 ccgctttcag gcccatatcc gccaggtcat cgatgttgac gcctatgata cctccccagg
    11581 acatcagctt gccaaaatgg acctccgagc ctgattgcaa ataatcatgg atgatggtct
    11641 tgcgcacacc agggatggcg gatatctcct taatagccgc gggtgtgagc agtttcatgt
    11701 ctgatccgcc gcctgcatac ataacgccgc cacctccacc ccctcccatc acccttgatt
    11761 cctggtatcc cgggtatact tcaatgctgg ttaggtcgct gatgccccat aattgctggg
    11821 tggcgctctt ctgcaacccc acagccagcg aaaccagcac caccaccgcg gcggtgccaa
    11881 tcaccacccc caccgcggtg agcagcaccc gccccttgcg cctgccaagg ttatagagaa
    11941 tcaggctgac gagatcgata aaacgcatcg cccgacctta ccctttaggg attgcgggta
    12001 ccgggataac ttctccactg ggtgtttctt gcactggata ggtctctacc ggctgctgga
    12061 gtccactatc cagcccaaag agccctttaa agaacctcac caccttttgc cagaaagtct
    12121 caggcgcggg tactacaggc ccctctccgt tcggcgtgtt gccatccccc atatctggcg
    12181 gtacttccat ggcaggctgg acctcaacgg gaataacctg ttcaatgaaa cgcggttggt
    12241 tgaaatcatc cgtgtaattg atgacaacct tcaggtccag cggcccctcc tggtaaggcg
    12301 tcaggctggc atccagggtg taataaccgc ccgggtccag cgcgccgacc agcaggatat
    12361 tattaagcag gtcggcgttc tccgcgctca ccttcatatt accgagcacg taggttttct
    12421 tgcccagatt ggtcacctgc agcggcagaa tgttttccat gccagcggta attatgcccg
    12481 cgtcacggta aaaactcacc tctacctggg gtaatgaata caccagcagg gtgatgacct
    12541 ggtcatccac caatcggtta cctttggcat cgatatagac aaaagacaac ttcagcgggt
    12601 aagctccggg ctgagcagta acattcacca ccagcttctg ggtcaccacc gcagtctggt
    12661 ctttgctgac gtcgcccata aagataacgt tggaactgcc cagcggcgcg aaagtggtca
    12721 ggtcactgct acctcccgat acgcctcccg gttgcggttt gccctcctca ttgctgggca
    12781 tacctccccc gccgatgaca gcggagacgt ttttcgcgtc cgccatgccc aggttcttga
    12841 catccagttt caattcaaaa atcgagccgg gctgcagagg atcaatgtcc gtgctgtagc
    12901 cagtcaccac cagttgtggt cgttgcggga tgttgggggt ggccgtagcg gcaaacccag
    12961 aagaagaggg ctcagcgatg acaaaagaga gggtgaagac ttcgctgtaa gcggtgccaa
    13021 gcgggtcagt gtagttcacc gtggctttca tactggccac gttggcccag gccagggcat
    13081 cgccaatgag aaatttttgg gtgatggtat tggtgttgcc cggggggagc gtgccggtgg
    13141 tgcgcacccc gccgctctca cgcggataaa agtcagtccc gtcgaaagtg acgatgatat
    13201 tttcagcgct taccttgccg ttattcccca gcaccacctg gaggttcatt tcattcccag
    13261 cgaagagcgt tttattgccc gtcgaataag aggtgatggt gaccatcggt ctcccgatag
    13321 cgggtttggg taccaccact gtggtcgtaa gctgcagggg gtcgaattct acaggattgc
    13381 ccgccactac tgttacgtcc accgttgtaa tgtcgctctg accctcagta gcgccaacag
    13441 ggatgggaac gttcacgctg accgtcttcg tctcattggc accaaaggta attgtgtttg
    13501 gaaccaccgt gggtgagttc caaccagaag tgctgttcgc agacacgttc attatttgcc
    13561 cagcctcacc aggaatgtcc aacttgtaag taataacaga acccggcaag ccgctcttgg
    13621 atttgctatc aactggcact tcatcgttca acggtgccgc gtatgtgttg agtgtggtca
    13681 ttccgaatag aaaggtcaag gtgagtaaaa ggcctaaaac attgcggttt tttttgatca
    13741 taggatctct tcctgttcta caatgggttg ggtcatattt tcggtggctt tgcgataagc
    13801 ctcttcagaa accaccttgc catccagcag gtaaagcatg ttggtagcaa actggcgcat
    13861 gcgcgggtcg tgcgtcacca cgatcaccgt tttgcctgcc tggtgcaggc gtgaaagcag
    13921 cttcatgatg gtgaacccgc tctggctgtc gaggttgccg gtcggttcat ccgccagtat
    13981 cagcggcggg tcgttgatta acgcgcgggc aacggctacg cgttgctgct gcccgcctga
    14041 aagctcggtg ggtttatggg tggcgcggtc gctcaagcca accttttgta aaatttcctg
    14101 ggcgcgcgcg tgcctttgcg ccctggggat gcgagcaaag cgcagcggga agctcacatt
    14161 ctcttccgcg ggcatggaag gaatcaggtt gaaggattgg aaaatgaacc ccacacggtt
    14221 gcgcctgtac tcagccagca agttttcgtc catcttttcg atctcgtcct cgccgattgt
    14281 gatactgcct gaagtcggcc aatccaaccc gcctaagagg tagagcaagg tgctcttgcc
    14341 tgaaccggaa ggtcccatca ccaccgtgaa cgaccctggg tcaatttcca ggctgacccc
    14401 atccagcgcg accacggtgt tttcacccat ggtatagcgt ttggtcaggt ctcgtgtgcg
    14461 taccagagcc ttatcgagca tccttatcct cactcctgtt agaagtagaa accgcctctc
    14521 aatgagagcc ctcccagggc tgaagcgagc aacccgggaa tggccatcag cagcagcacg
    14581 atcaccacag aaattgccgt ggcagtccag gctttggaac gggataaacc ggaaagcgga
    14641 acggagccga gcagcagcaa ggcaatttga aagaggaagt agatgtcgat aaaaccgagc
    14701 atgctgcgca agtaagcccc aaccccggtg gcttcagcag cgataaaacc agaaagacct
    14761 ggggcgctca ccactgtttt gctcactatc atcgccaaaa tctgtatcag caaacggacg
    14821 gccatgggca gcatggacca tgccaccagg ttatacgatt tgatactgcc agcgcggctg
    14881 cccgccagcg taaggctgag gtacatcagg atgctgagca gaaaccaggg catccagacc
    14941 ttcagcagcc cggaaattaa cgggaacacg tacaggaaca acggagaagt ctgctttgct
    15001 tgggcggcaa aaaactgttc ctgcatctcg ggggtgtagt attggaagtc aggcggaagt
    15061 tcactgccca tctggatgac attccgcttg atgggagcac cgatggccac cgccagaatc
    15121 accagcacag agagaataag cagaggggtc agccatgccg ctttttcctg cgtcacgatt
    15181 tcagaagtgg tgcgcgccgg gcgcgtgaat aaggagggaa tccattttgt gttccaggag
    15241 tgtttattgg tttgttgacc tttcgcattt aatgccaaag cgcacctctt tttagggttc
    15301 tttcagcata agacgcattt atcatactac aagttcccgc ggcagccgtt atcaaaaaaa
    15361 tcgttatgct ataatcgcca aaggagggca ccatgcgcat caatattggt gtggagaaca
    15421 attacgaagg cagatcgctc gcctggatgt tggattaccc gggttgtttt gcctacggcg
    15481 ctgagggtag cgaggccatc ctccgcgcgc cgcaggcgct gctgcgttat aaagaatggg
    15541 ttgacagtca cctgaaagac tcctggatga aaagcctggg tgatttcgac atccacctgg
    15601 aagaaagtta tgacgttttc agcatcaatg atgatttcga cctcgcagac gaagggattg
    15661 aggtgaacgc ctggttccgt cacgactgga aacctctcac tgcagaagat atccgccggg
    15721 ggacgctggt actaacctgg agccgggaag acctgctcga gctggtttcg gggttaactc
    15781 ccgcccaatt ggactgccgt ttttcaggcg agcgttggag cattgccggt gtgctggagc
    15841 atatcgccaa cgctgaacac tggtaccttc accgccttgg cctggcggtt ggaacccgcc
    15901 aggaactgcc cgcggatgtt tttgagcgcc tgaaggtggt acgccgccgc ctggtagagg
    15961 tgctgccatc cttgcaaggc agccgccagg tgcttggggt ggatggagaa ttctggtcgc
    16021 ctcgtaaagt actgcgtcgg tctgcctggc acgaagtcga tcacattgaa catatcttca
    16081 agttaatctc caggctataa aaaacgcctc cgcgcggagg catttttttg cgaacatcag
    16141 gagggaggtt gcgggtcgtt ttacttgagc gaagtatcaa gcccaagcag gctgaacgcc
    16201 agaaacatca aaccaaatac ggctatcacc agatacacaa tcatcgccat cgcacacctc
    16261 caatttaacc tttctctcgc gctcctttta accagtcaat ttctcattaa ttgatcttat
    16321 tcgccttctt aaatacgtat aaccccgata aaagttcccg ggtgctggtt ggatctcgga
    16381 aatagacgcg cttaataaat ttgtaaactc taacgggaaa ccgccatcat ccgttggcgt
    16441 ggcctgcttg cggtctgccc gaccaaaccc cggcaatggg ctgaccgcaa aaggcgcagg
    16501 cgccggcgct gttgatatga tacccatgca gcaggtaacc gcgcctttcg atcaccgccc
    16561 tgccgcactg cgggcaaatg gtgttttcca gcgagcccac gcgccccgga aggtttccag
    16621 cgtaaacaaa acgcagcccg gcttcctgac cgatctcggc ggcgcgctgc agcgtttccg
    16681 ccggggtggg gggcgcgttt tctttgtaat ccgggtgata tgccgtcaca tgccagggaa
    16741 tatccggcga aatggaagcc aggaaacgac ctgcctccca aagctcatct gaagaatcgt
    16801 tcatcccggg tattaccagc gttaccacct ccaaccacag ccccaactct ttgatgcgcc
    16861 ggatggtatc cagcactggc tgcagtcggc cgcccatctt cctgtactcg ctgtcgcgca
    16921 tgcttttcag gtctacttta tagccgtcca gccagggcac caggtagtcc agcacttctg
    16981 cggtggcgta accgttagaa acatacgcgg ttttcagccc tttgtctttg gcgagtttaa
    17041 acacctcaac tgcccattca gtggtaatga gcggctcatt ataggaggag gcgatggccc
    17101 gcgcgccatt ttctacagcg taggtcacca gcttttcggc ggaaacccgc ctgatagcat
    17161 aggccgacct gccggcttca gggtcacgca gcgcctgcga agagagccag ttctgacaga
    17221 aaccacaatg aaagttgcag cccaacatac caaaggtcaa cacttcgctg ccgggtaaaa
    17281 aatggttgaa gggctttttt tctagcgggt cgatctgcac gccggccaca tatcctttcg
    17341 gggccattaa aacgccgttg gcgttgaacc gcacgccgca aataccgcgg cgcccgggtt
    17401 tcagcaagca gcggtgcgcg caagccagac agcgcaggct gccctgttcc agtgtttcca
    17461 ccagttctgc gggctgggtg agcacatcga gtaattcatg cgggttcatc tgtcgctcca
    17521 tatcca
//
LOCUS       DAOTLW010000037        17094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_3748_length_17094_cov_10.481777, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000037 DAOTLW010000000
VERSION     DAOTLW010000037.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 17094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 17094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17094
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3748_length_17094_cov_10.481777"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(419..491)
                     /locus_tag="P5516_06680"
     tRNA            complement(419..491)
                     /locus_tag="P5516_06680"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(456..458),aa:Lys,seq:ctt)
     gene            complement(550..1260)
                     /locus_tag="P5516_06685"
     CDS             complement(550..1260)
                     /locus_tag="P5516_06685"
                     /inference="COORDINATES: protein motif:HMM:NF013918.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferric reductase-like transmembrane
                     domain-containing protein"
                     /protein_id="HRT92156.1"
                     /translation="MRIEPETSISHDHTSTEGGVQPRRKRIKNRGRLVVNLFAAVVFL
                     ILLAGYLAGWLGEYPARNAQLITGRIAFLFLLASLSITPLRTLTGNIHIGPLRQAFGL
                     NAFYFAMVHVLILIFLNYRMDFYALVKGFSNHPYFWPGAVAFLILSVLAVTSLNKMKR
                     ALRGSWKKIHTMVYPAGVLVLVHYSWIANGKFIPAAEVKTLPLLTAAYLAALFTLRLR
                     PVKKAVLRWRKKAKPRTG"
     gene            complement(1351..2577)
                     /locus_tag="P5516_06690"
     CDS             complement(1351..2577)
                     /locus_tag="P5516_06690"
                     /inference="COORDINATES: protein motif:HMM:NF017760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92157.1"
                     /translation="MLEHKLSQTFSSLQHPNFRLWFGGQIVSLIGTWMQATAQGYLVY
                     ELTASPMMLGIVGFANGIPVWLFSLFAGTVADHISRRKMLLITQGSMMVLAFILAVLT
                     FSGTVRAWHIILLALLLGTANAFDAPGRQSFVVDLVPKQELTNAIALNSSVFNLGTII
                     GPAAAGLVYAWLGPGWCFTINGLSFIAVLLALALMKIAAKPPLRPAQGSLAHTLTEGV
                     RYSFKDANIRVLLVMLTASAVFGFGLLALMPAWATGVLGGDVRTNGLLLSARGLGSLS
                     AALMIAYLGARIIRGRLWSIGSLVMPVSLLLFALFRLLPVSLLMLVLMGWSLMTVVNL
                     TNALIQTHVPDELRGRVMSVYILLFQGGFPIGSLVAGWLAGLTSEALAVFVFASVLLA
                     VSIAIQLRQPSIRQYN"
     gene            3167..3769
                     /locus_tag="P5516_06695"
     CDS             3167..3769
                     /locus_tag="P5516_06695"
                     /inference="COORDINATES: protein motif:HMM:NF013077.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HRT92158.1"
                     /translation="MEDTLTVIMKRRSIRKFTDQPVEKEKIIQLLEAAMAAPSAMNAQ
                     PWEFVVITEKSVMDKFRRALMFAKMNAPAAICVLGSSRMQKGKVGDKFWVQDCSAATQ
                     NILLAATALGLGSIWVGVHPVTIFSRQVSHILNLPKGVTPLNLIYIGYPAETKEPRTQ
                     YEEARVQWGPFPPAEKKHAKTFPQDQSILLDEQSNDHTDQ"
     gene            3750..4193
                     /locus_tag="P5516_06700"
     CDS             3750..4193
                     /locus_tag="P5516_06700"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT92159.1"
                     /translation="MTIQINEMSVLHIQAALALWKQIEGMGLSSADEPGALARFIQQN
                     AGLSYIAEEDGQLVGTCLCGCDGRRGYLYHLAVLPQYRRRGLGSALVRTVFTALHKRD
                     IHKCHIMVYTTNHSGLKFWQADGWVRRPEIVLMSKEIEVRDGPAA"
     gene            4205..4711
                     /locus_tag="P5516_06705"
     CDS             4205..4711
                     /locus_tag="P5516_06705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92160.1"
                     /translation="MKASKVAIILSLCLWVLTACNGGEQAGLNQETGVFPKTDWSTIP
                     EQLPASLKGYELYSWQQGKTRVYTLTTGTNRTKSFAEITAAENIWEGDYLKISVASLE
                     ELKKLLSRLPAETQVFWGGIDLSGQVEEGTLYFSYPADEELKEILQYSQELGLKLHTI
                     HEQQEDEP"
     gene            4708..5712
                     /gene="add"
                     /locus_tag="P5516_06710"
     CDS             4708..5712
                     /gene="add"
                     /locus_tag="P5516_06710"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009168 - purine ribonucleoside
                     monophosphate biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HRT92161.1"
                     /translation="MISQDIPLVELHRHLDGSIRLETILELGLKHNLPLPGKTLDTLR
                     PFVQVTTPQPGVMAFIEKFKWMTGVMVDYDSCRRIAYENVEDACREGIDYIELRYSPW
                     FMAEAHGLQPEGVVEAVTEGIRAAEQEHPIRVNQLGILSRQYGPQIAMKELEAELRYA
                     HEFAGLDLAGDEEGFPGELFRDHFRKARDAGWHITVHAGESAGPESIWQAVRELGAER
                     IGHAVRAPEDPALLDFLREKGIGVECNLTSNVQTSVVSDYAAHPMRLFMEKKILASIN
                     SDDPGISAINLRYEYEVAAPKAGLSPAMIRQAQENALLTAFLTEEEKTALRRKKSTGA
                     "
     gene            5760..6698
                     /locus_tag="P5516_06715"
     CDS             5760..6698
                     /locus_tag="P5516_06715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HRT92162.1"
                     /translation="MRYLITGAAGFLGSALANRLTAAGDVVIGVDDLSAGDQKNLSPT
                     VQFVRGDVNDRPTLWSLLQEVDCVYHLAAKVSVQESVLYPREYNNVNVGGTVTLMEAM
                     RDVGVRRVVFISSGTVYGNQPVQPVKETVIVNPRVPYAVSKLAAEYYVKTIGSLWGIE
                     TVCLRVFNAYGPGQRIPPVHTPVIPGFLHQAWENGTIVIHGDGNQTRDYVYVDDVVNA
                     MLAAANAPDVNKLTINVGSGTETSVRDLARMAIEVTGGNPEVVYNPRNEGGISRLRAD
                     ISLARELLAYEPEVELLEGLKRTLAHDTATDKTQYE"
     gene            6774..7379
                     /locus_tag="P5516_06720"
     CDS             6774..7379
                     /locus_tag="P5516_06720"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRT92163.1"
                     /translation="MGIILDMNCLPQSFYLGEVTGVARALLGKRLVRQVDGRRVSGII
                     CETEAYRGEDDLACHAHSGKTRRNEVMYGLPGRAYVYFTYGMHWCLNTVCGEEGYPAA
                     VLIRAILPEEGLEWIAARRAGVEPANWCNGPAKLTKALGVDGGLNGTDLTDANSPLFI
                     EEGYIIEQAQIRTSPRVGIQSAPEPWRSMPWRFTCSCRAGT"
     gene            7379..8275
                     /locus_tag="P5516_06725"
     CDS             7379..8275
                     /locus_tag="P5516_06725"
                     /inference="COORDINATES: protein motif:HMM:NF012880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HRT92164.1"
                     /translation="MDEITLYRTMYRIRRFEETVLEEFKRGVFAGTTHTSIGQEANAA
                     GVIAALDPQDIIVTNHRCHGHFIAYGGDPRALFAEMMGRQTGVCGGRGGSQHIHWRNL
                     YSNGVQGGIMPIAAGMALAEKEKKSGAIVTVFIGDGTFGEGVLYEACNMASLWKVPLL
                     IVVENNHIAQTTPTTLAMAGNIAGRLQAFDIPVTELDSCDVMEIHLNAMQMTRLVRRE
                     GRPYALVLNTARFGPHSKGDDTRAEAEIQALRSSRDPLLISGSRLAKDELERFQGETD
                     EEVNGAFTRALADPPARLEEGG"
     gene            8272..9321
                     /locus_tag="P5516_06730"
     CDS             8272..9321
                     /locus_tag="P5516_06730"
                     /inference="COORDINATES: protein motif:HMM:NF014798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HRT92165.1"
                     /translation="MNTTVLSSLNAGLLRALQKDEHVYLLGEDILDPYGGAFKVTKGC
                     SSAFPERVLAAPISEAGLAGVSAGMALRGLRPVLEIMFGDFITLTADQLINHISKFNW
                     MYDHSVCLPMVIRAPMGGGRGYGPTHSQTLEKLFIGIPGLTLIAPLNLTGKGGTQQPG
                     ELLEEVILHTEEPVLFIENKLQYLLPVYQPQDLPELHWLVEQPVKNTLMKTPTYLVKV
                     SGAPAARASLAAYGYMAELARQAMLKLAYEDEIFVELVIPTRLAPFDFTALADSIKRT
                     GRLVTVEEGNLTGGWGAEVMARALEEVGGRLRAAVRVAARDLPVAAAPSLENEILPDV
                     EDIVQAVRKVVGRNE"
     gene            9314..10402
                     /locus_tag="P5516_06735"
     CDS             9314..10402
                     /locus_tag="P5516_06735"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NeuD/PglB/VioB family sugar acetyltransferase"
                     /protein_id="HRT92166.1"
                     /translation="MSEPLQILVPLINPNEPEALLAELRVREGDKVTAGQVLASFETT
                     KSTYELTAERDGFILNLRFKAGDTLRSGERLCYLADSIGQPLPHETAKPAADRLPRQE
                     ELPQGLRITQPALALAKTNQVDLEDLPRGVLVTEEMVRKMRPIPVEDVDPSALIIYGG
                     GGHAKSLIDLIRLLGQYRIHGILDDGIAAGTRILGIEVLGGAELLLGLRRQGLGKAVN
                     AVGGIGSIVPRLAVYEKLAAAGFECLTVVHPRAFVEPNARIAEGCQVFFNAYVGSEVE
                     VGFGSIINTAAVISHDCRLGKTVNVSPGAMLAGAVTLGDRALIGMGATINLGVTIGAG
                     ARVGNSAVVKADVPENGIVRAGATWPAD"
     gene            10524..11009
                     /locus_tag="P5516_06740"
     CDS             10524..11009
                     /locus_tag="P5516_06740"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRT92167.1"
                     /translation="MFKKPTPATQEQTAPIERVNSVLGPGINWAGNLGGRGGIRIEGT
                     FEGEIRIQGLVVVGETGRVTCQNLRANSVIVAGALRGNVTAEKLEIRSTGRVWGDVVT
                     IAFATEEGAFLRGQVRMEEKVDLHLEEPSEAVKDLDEPADEKRMDTPKRPARGRQVAG
                     D"
     gene            11015..11764
                     /locus_tag="P5516_06745"
     CDS             11015..11764
                     /locus_tag="P5516_06745"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HRT92168.1"
                     /translation="MDVKFWLGYLSEWLGVIAVVMIAGTSPLLRKIRRIEFRFSRREA
                     AFALTLFALIFFFAFQVYDNNLLGFLKTAAGAFVGGDVALRMLIAVIALLPAIILLLA
                     RGQPLKSTGWSKENTRASLMLGLLLAVLTIFLRGKFTPLLKGFTEEKGSLLLVLLILS
                     LAEESIFRGYIQLRLMSYLGTTWGWLATAFLYLLWQLPGRAWLSHFPTAWPEVLITLA
                     QALILGWIMRKSYHVAATALYRAVAAWLMVI"
     gene            11761..13530
                     /locus_tag="P5516_06750"
     CDS             11761..13530
                     /locus_tag="P5516_06750"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF006625.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HRT92169.1"
                     /translation="MSLFNYLEQRMRLSNLFSQTLRENPADAEFASHQLLVRAGFIRQ
                     LGSGIFTYMPLAYRVLERVKRILREEMEAIGGQELLMPVVQPADIWKESRRYYQIGSE
                     MGRFTDRSGHEMVLAMTHEEAVSDLVRGVVHSYRQLPAIIYHLQTKWRDDPRPRAGLI
                     RVREFLMLDSYSLDADWQGLDTRYKQHYEAYFRIFRRCGLPVVAVRSDSGMMGGEEAH
                     EYMYLTPGGEDSLLFCDACGYSANRQAATTRKMEPAKEAFLPLEKVATPRVSSIDDLT
                     AFLNIPPARTAKAVFYMGLVEEDGGEQKWAFIFTVVRGDMEVNETKVANLVKASDLRP
                     ATEDEIRSIGAVPGYASPLGLKGVTVFVDELAAKSANLVAGANEEGYHLRNVNHGRDY
                     QASMIADISAAREGDACPECGAALRLSRGVEVGNIFKLGARYSDALGCTYLDKNGQSQ
                     PVIMGSYGIGLGRLIACVAEEHHDERGLAWPVSLAPFPVHVVVLKGKEMDSEALAARV
                     ENALRDAGLEPLVDDREESAGVKFNDADLIGLPLRITVSERSFRVGGLEIKPRRGGET
                     VVVPMENLVVEVQRLLNALEEPL"
     gene            13571..14758
                     /locus_tag="P5516_06755"
     CDS             13571..14758
                     /locus_tag="P5516_06755"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRT92170.1"
                     /translation="MKNVEPVVPMSLPDLSDAEREAVMEVLRTPHLSMGPRIDDFEAA
                     LAEFVGAKEAIGVSSGTAGLHLCVRAAGIQPGDLVLTTPFSFVASTNAILFEGGIPVY
                     VDVDPKTGNMDPLLAAQALEDLRKGGAAARRWLPRKGAGDTARLKALLPVDVFGQPAD
                     YDLLQPLAEEFHLKHIEDSCESLGAEYKSRRLGLMGDYGVFAFYPNKQMTTGEGGIIV
                     TDDADAARYMRALRNQGRAPGDTWLQHTYLGYNYRMDEMSAALGLAQLRRIEELLNKR
                     QQVADWYASRLANIDGVQIPLIAPETTRVSWFVYVIRVAPGLDRDALAEKLQLRGIPV
                     RPYFLPIHLQPYMVDKFAYQLGDYPVTEDLGRRGLALPFSGKMTEEQVDLVCRVLAQC
                     VTK"
     gene            14772..15683
                     /locus_tag="P5516_06760"
     CDS             14772..15683
                     /locus_tag="P5516_06760"
                     /inference="COORDINATES: protein motif:HMM:NF022338.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PH domain-containing protein"
                     /protein_id="HRT92171.1"
                     /translation="MSQRQAFYPPKKAGILLHAFLSLLLLGGIAFLLVLAFSQPPSWR
                     LVFYLLGVILLGMLLPLAAYRGYALLKADYSLERDGLQVRWGLRSEDLPVTDVLWVRP
                     ASDLVKPLTLPRFALPGAILGTSHHEELGEVEFIASSARHLVIVAALEKTLILSPADP
                     EDFSRRFQRVIEMGSLSPLPAHTTIPAAYMNQVFKDPLAKVLMVLSAVLTLLLLLATG
                     LLIPSREVVSIGYDFNTLPMPPVPSNRLLMLPVIALFFVLLDFIAGLFLYRRAETRAI
                     SYLIWAAGILSPLLLLIAVLLTTMVRV"
     gene            15685..16521
                     /locus_tag="P5516_06765"
     CDS             15685..16521
                     /locus_tag="P5516_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF92 domain-containing protein"
                     /protein_id="HRT92172.1"
                     /translation="MGVPLSPIQLIFGVLLAALVVYAAYRARSLDRSGALAAFLLGVV
                     VFGIGGFCWALVLLTFFITSSGLSHLFRVKKQKVEEQAVKGGRRDAWQVGANGGVAGL
                     AALLSLVMPGSVLPWLAFAAAFAAANADTWATELGVLNRSWPRRLTDWKRVPPGTSGA
                     VSLAGTLAATGGALLVALMAAACWPVVNTSFREKLVWGLVILLAGVFGSLVDSLLGAT
                     LQVIYWCPACEKETEKHPLHSCGKPTRRVRGWGWLNNDVVNLACTLSAALLACLLGLL
                     LR"
     gene            16637..>17094
                     /locus_tag="P5516_06770"
     CDS             16637..>17094
                     /locus_tag="P5516_06770"
                     /inference="COORDINATES: protein motif:HMM:NF019428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HRT92173.1"
                     /translation="MNFMLLWLRSKDRRIAKPAQGLVEFALIIPLFLMLLYGLFEVGR
                     AVFMLSAVRNASRDAVRYAGASGTNPSGVERYRDCAGIRDRAKRVSAFVDLSAVNAVE
                     ISYDKGPSTPPISCEALVSSGKKLALGDRVVVTVRATFVPFVGPLLNFNT"
BASE COUNT         3840 a         4458 c         5156 g         3640 t
ORIGIN      
        1 tcaccttaag gatacttctg actcaccgct tttgttaagg ttcaacaatt tacaggaaat
       61 atgttacacc aactactttc cttataagat tcagattatt cgcggcaaga atttatattt
      121 atagtgatta atttaatcca cagcctcggt tattcaccac aattttccaa gagagactgg
      181 gaaatgactc atcaaaatga gtgcaacctg tctgcaggga cgattgaaga gatattgcgc
      241 aacagattag aggctgttcg ggagatggtg tgggtgctgt tcaacagtgt cacacaggcg
      301 gaaagaccaa gtacctgcaa gccggagaat acgaacgcag cgcatacccg aaaggtcata
      361 ccaacgggta taaaccgata ttttggtggt tgttcaactc aattaccccc acatttagtg
      421 atccaccggg gacttgaacc ccgaacccac tgattaagag tcagttgctc tgccaattga
      481 gctagtggac cgttcttcgg gggttattat atcctaaaaa gaaacactgt caaacaacac
      541 cagccacgct caacctgtgc ggggttttgc ttttttccgc cagcgcaaca cagccttttt
      601 cactggcctt aaccttaagg tgaacagcgc cgccaggtag gcagccgtta gcagcggtaa
      661 ggttttcacc tctgcagcgg gaatgaattt cccgttggct atccatgagt aatgcaccag
      721 caccagcacc cctgccgggt acaccatcgt gtgaattttc ttccatgaac cacggagggc
      781 tctcttcatc ttgttgagcg aggtcacagc caatacgctg aggattagaa aagcgaccgc
      841 tcctggccaa aagtatggat gattggaaaa acctttgacc aaagcataaa aatccattcg
      901 gtaattcagg aaaatcagga ttaatacgtg aaccatcgcg aaatagaagg cgttcaagcc
      961 aaaagcctgt cgcagcggcc caatatggat gttcccggta agcgtgcgca ggggagtgat
     1021 actcagcgaa gccagcagga aaaggaaggc aattctgccg gtgatcagct gagcgtttcg
     1081 cgccgggtat tctcccaacc aacctgccag gtaacccgcc agcaggatca ggaaaacgac
     1141 cgcggcgaaa agattgacga caagcctccc cctgttcttg attcttttcc gcctgggctg
     1201 aacacctcct tccgtggagg tatggtcgtg gctgatggaa gtttcaggtt caatgcgcat
     1261 ctcgaggttt tattaacttt cttgaaaaac aggatgaaac caaccagtga acattataag
     1321 cgatgtgcac gaacgccggt catcaatgca tcagttgtac tgccgtatgg agggttggcg
     1381 cagttggatg gcgatggaca cagccagtaa tacgctggcg aacacaaaaa cggcgagggc
     1441 ttcgctggtc agccctgcca gccaaccagc gaccaatgaa ccgatgggga agccaccctg
     1501 aaacagcaga atgtacacac tcatcaccct gccgcgcagc tcgtcgggga cgtgggtttg
     1561 gatgagcgcg ttcgtgaggt tgaccacggt catcaggctc caacccatca ataccagcat
     1621 cagcaaggag accggcagca gacgaaagag ggcgaacagc aatagcgaaa caggcatcac
     1681 caggctgccg atgctccata acctgccacg gatgatgcgc gcgcccagat aggcgatcat
     1741 cagcgctgcg ctcagcgaac ccaaaccgcg cgctgaaagc agcagaccat tggtgcgcac
     1801 atctccgccc agcacccctg tcgcccaggc gggcatgagt gccagcaatc caaagccaaa
     1861 gaccgcgcta gcggtgagca taaccagcag aacgcggatg ttcgcgtctt taaacgagta
     1921 gcgcactcct tcggtgagtg tatgagccag tgatccctgc gcagggcgta aagggggttt
     1981 ggcagcgatt ttcatcaacg ccaacgccaa cagcaccgcg ataaatgaaa gcccattgat
     2041 ggtgaagcac catccaggac ccagccaggc gtaaaccaaa ccggcagccg ccggaccgat
     2101 gatggtgccc agattaaaca ccgagctatt gagagcgatg gcgttggtga gttcctgttt
     2161 gggcaccagg tccaccacaa atgattggcg accgggagcg tcgaaggcat tggccgtccc
     2221 caacaggagc gcgaggagga tgatgtgcca ggcgcgcacg gtgccgctga aggtgagcac
     2281 cgccaggatg aaagccagaa ccatcattga accctgggtg atgagcagca tttttcgccg
     2341 tgaaatatgg tcagcgacgg tgccggcgaa aagcgagaac aaccacaccg gtatgccgtt
     2401 ggcgaagccg acaataccca acatcatggg agacgcggtc agttcgtata ccaggtatcc
     2461 ctgggcggtg gcttgcatcc atgtaccgat gagcgaaacg atctgaccac cgaaccataa
     2521 acggaagttg gggtgctgca aagaagaaaa ggtctgtgat agcttatgct ctagcatctg
     2581 cagattattt taagagtatc ctgatattgc gcaattccta actgactgcc cttgcgcaaa
     2641 atccagcgcg ctaaaaaaac cgctaatgat tggctacccg taaaagaaag gggggggcaa
     2701 aatgatctcc cggctgccaa caaaaaactc ttataataag attggctctg agctggtccc
     2761 aatggaactg gagggcgatg agtgagatga tgcgagaact tgcttatagc tgcattttca
     2821 tgaaaaggat tggatggggt tatacacaca ccctcaccct gatgcccggc ggaaggcttg
     2881 gagggatgga acactggatg gcggtcaggc gcgcaaaacc cgactaccta tgcacaccct
     2941 cctcgtgccc gaaaatcgga caatgatgga ttatcctttc ccgtggattc cccgatgttt
     3001 tacagcgaat ttatgcgctt atcccagcaa acacttcggg ctgaaggata ggaaaaaagt
     3061 cggttccgcc tgatcattaa tgacggcgaa tcccaatcga tcctgcagct gcatgttcac
     3121 ttggccgctg gcgagccaaa ccgcgaggta aaaaaggagt aatactttgg aagatacact
     3181 taccgtgatc atgaaaagac gcagcattcg aaaattcacc gaccagccgg tggaaaagga
     3241 aaagatcatc cagctgctgg aagcggctat ggctgcccca agcgcaatga acgcgcagcc
     3301 ctgggagttt gtggtgatta cagagaagtc agttatggat aaattccgtc gggcgttaat
     3361 gttcgcgaaa atgaacgccc ccgcagccat ctgcgtgctg ggcagcagcc gcatgcaaaa
     3421 agggaaagtg ggcgacaagt tttgggtgca agattgcagc gccgccaccc aaaacatcct
     3481 gctggcagcc accgcgcttg ggttggggtc tatctgggtg ggcgtgcacc ccgtgaccat
     3541 ttttagccga caggtgagcc atatcctcaa cctgccgaaa ggcgtgaccc ccttgaacct
     3601 catttacatt ggctacccgg cagaaacaaa ggagccgcga acgcagtacg aggaagcacg
     3661 cgtgcagtgg ggtccgttcc cgcccgcaga aaagaagcac gccaaaactt tcccgcaaga
     3721 ccagtctatt cttttagacg agcaatcaaa tgaccataca gatcaatgaa atgtccgttc
     3781 tccatattca ggcggcgctt gccttatgga agcaaataga aggcatgggg ttgagcagcg
     3841 cagatgaacc aggcgcgctc gcccgtttta tccagcaaaa cgccgggttg tcgtatatcg
     3901 cagaagagga tggtcaactg gtgggtacct gcctgtgtgg ctgcgatggc aggcgaggtt
     3961 acctctatca cctggcggtt ctacctcagt accgtcggag ggggctgggc agcgctttgg
     4021 tgcgcaccgt cttcacggct ttacacaaga gagatattca taagtgccac attatggtct
     4081 atactactaa tcatagcggt ttaaaattct ggcaggcaga tggttgggta cgcagaccgg
     4141 agatcgtgct gatgtcgaaa gagatcgagg tgcgcgatgg tccggcagct tgatgaggga
     4201 agcgatgaaa gcgagtaaag tggcgataat cctcagcctg tgcttgtggg tgctcacagc
     4261 ctgcaacggc ggagaacaag cagggctgaa ccaagagact ggggtattcc ccaaaacgga
     4321 ttggtccacg atcccagagc agttacccgc gtcactgaaa gggtacgaac tgtattcttg
     4381 gcaacagggc aaaacacgcg tatacacgct taccaccggc accaatcgca ccaagtcttt
     4441 cgctgagatt acggcggctg agaatatttg ggagggcgac tatctgaaaa tttccgttgc
     4501 cagcctggaa gagctgaaaa agctgctcag ccgcctgcct gctgaaacgc aggttttctg
     4561 gggcggcatt gacctctcag gacaggtgga ggagggaacc ctctactttt catacccggc
     4621 agatgaggaa ttgaaagaga ttcttcaata ttcgcaggag cttggcttaa aactacacac
     4681 tattcacgaa caacaggagg atgaaccgtg atttcacaag atattccttt ggtcgaactg
     4741 caccgccacc tggatggcag catccgtttg gaaaccatcc ttgagctggg gttaaaacac
     4801 aacctgccat tacctggcaa aacgcttgat actctgcgcc cgtttgtaca ggttaccaca
     4861 ccgcaaccag gggtgatggc gtttattgaa aagttcaaat ggatgaccgg cgtgatggtg
     4921 gattacgact cctgccggcg catcgcctat gagaacgtgg aagacgcctg cagagaaggg
     4981 attgattaca tcgaactgcg ttacagcccc tggttcatgg cagaagccca cggcttgcag
     5041 cctgagggcg tggtggaagc cgtcacggag ggtatacgcg cggcagaaca ggaacacccc
     5101 attcgtgtca accagttggg aatcctttca cggcaatacg gaccgcagat tgccatgaaa
     5161 gaattggagg cggaactcag gtatgctcat gagtttgccg ggttggacct ggctggtgat
     5221 gaagagggct ttcccggcga attgttccgc gaccatttcc gtaaggcacg agacgctggc
     5281 tggcatatca ctgtgcacgc cggagaatcc gccgggccgg aatcgatctg gcaggcggtg
     5341 cgcgagctcg gggcggagcg tattggtcac gcggtacgcg cgccagaaga ccccgcgttg
     5401 ctcgattttc tgcgtgagaa aggcatcggc gtcgaatgca acctcaccag caatgtgcaa
     5461 accagtgtgg tctcggatta tgcagcgcat cccatgcggc tttttatgga aaagaaaatt
     5521 ctcgcctcta tcaactcaga tgaccccggc atcagcgcca tcaacctgcg ctatgaatat
     5581 gaagtggcgg cgccgaaggc aggattaagc ccggcgatga ttcgccaggc acaggaaaac
     5641 gctcttctca ccgcttttct gacggaggaa gagaaaacag ccttacgtcg aaaaaaatcc
     5701 actggggcat aaatccgtct atgctataat gcaggcactt aaataaggaa aaaggctgga
     5761 tgcgatatct cataaccggc gcggccgggt tcctgggttc agcgcttgcc aaccgcctca
     5821 cggctgcagg cgatgtggtc atcggggtgg atgacctttc cgcgggtgat caaaaaaatc
     5881 tttcaccaac ggtgcagttt gtacgcggtg atgtcaatga ccgaccaact ttgtggtcgc
     5941 tcctgcaaga agtggattgt gtctatcacc tggcagccaa ggtgagcgtg caggagtcgg
     6001 tgctgtatcc acgtgaatat aacaacgtta atgtaggcgg tacggtgact ttgatggaag
     6061 ccatgcgtga tgtcggcgtc aggcgcgtgg tgttcatttc ttccggcact gtttatggca
     6121 accaaccggt gcagccggta aaagagaccg tcattgtcaa cccgcgcgtg ccatacgcgg
     6181 tttcaaagct ggcagcggaa tactatgtaa aaacgatcgg ttccctgtgg gggattgaaa
     6241 cggtatgctt gcgggttttc aatgcatatg ggcctggtca gcgcattccg ccggtgcaca
     6301 ccccggtgat acccggcttc ttacaccagg cgtgggagaa cggcaccatt gtgatccatg
     6361 gtgacggcaa ccagacgaga gattacgttt acgtggatga cgtggtaaac gccatgctgg
     6421 ccgcggcgaa cgcccccgat gtgaacaagc tcacgatcaa tgttggcagt ggtactgaaa
     6481 cttctgtaag agacctggcc agaatggcga ttgaagtaac cggcgggaac cctgaagtgg
     6541 tatataaccc gcgcaacgag ggcggcataa gccgtttacg cgctgatatt agcctggcgc
     6601 gtgaattact ggcgtacgag cctgaggtgg agctgctgga aggattaaaa cgcacgctgg
     6661 cgcatgacac cgccacagat aaaacacaat acgaatgatc aatgcaagca gacaagtgat
     6721 ttttcaaaca ccacccgggc gggtggcgtt tgattaaagc gggaaggtgc tgtttgggta
     6781 taatacttga tatgaattgc ttgccgcaat ccttttacct gggtgaagtg actggcgtgg
     6841 cgcgcgcgct gctgggaaag cgcctggtcc gccaggtgga cgggcggcgc gtctcgggga
     6901 tcatctgcga aacagaagcc taccgcggag aggatgacct tgcctgccac gcgcactcag
     6961 gtaaaacccg gcgcaatgag gtaatgtatg ggctgccggg tagagcgtat gtctacttta
     7021 cctatggtat gcactggtgt ctgaataccg tgtgcggtga agaagggtat cctgcggcgg
     7081 ttttaattcg cgccattttg ccggaagagg gattggaatg gattgccgcg agaagggcgg
     7141 gggttgaacc cgccaactgg tgcaatggcc cggcgaaact aaccaaagcg ctgggggttg
     7201 acggtggctt gaatgggacc gacctgacgg atgcaaacag tccgcttttc attgaagaag
     7261 gatatatcat agagcaggca caaattcgca cttcgccgcg ggtgggcatt caatcagcgc
     7321 ctgaaccctg gcgttcaatg ccctggcgct tcacctgttc ctgccgagcg gggacttgat
     7381 ggacgaaatc acgctatacc gcaccatgta caggattcgc cgttttgagg aaacggtact
     7441 cgaggaattt aagcgcggtg tgttcgctgg caccacgcac acctccatcg gacaggaagc
     7501 caacgctgcg ggagtgattg ccgcgctgga cccgcaggat atcatcgtca caaatcaccg
     7561 ctgtcacggg cactttatcg cctatggcgg agacccacgg gcgttatttg cggaaatgat
     7621 gggacgtcag acgggcgttt gcggcggcag gggagggtcg caacacattc actggcgcaa
     7681 tctctattcc aatggcgtgc aggggggcat catgcccatt gcagccggca tggcgttggc
     7741 cgaaaaagaa aagaaaagcg gggcaatcgt gaccgtgttc attggtgatg gcactttcgg
     7801 agagggcgtt ctctacgagg cgtgcaatat ggcctcttta tggaaagtgc cgttattgat
     7861 cgtggtggaa aataaccaca tcgcgcaaac cacgcccact acgctggcga tggcgggaaa
     7921 tatcgccggg cggctacagg ctttcgacat accggtaacc gagttagaca gttgcgatgt
     7981 gatggaaatc cacctgaatg cgatgcagat gaccaggttg gtcaggcgtg aagggcgacc
     8041 atacgccctg gtgctgaata ccgctcgttt tggcccgcat tccaaaggag atgacacccg
     8101 cgcagaagca gaaatacagg cgctgcgctc gagccgcgac ccgctgctca tcagcgggtc
     8161 aagattggca aaagatgagc tggagcggtt tcagggggaa accgatgaag aggtgaacgg
     8221 ggcttttacg cgggcgctgg ctgacccgcc ggcgcggctc gaggagggcg gatgaacact
     8281 accgtgcttt cctcgttgaa cgccgggttg ctgcgcgccc tgcaaaaaga tgaacatgtg
     8341 tacctgctgg gtgaagatat cctcgaccca tacggcgggg cgtttaaagt gacgaagggc
     8401 tgctccagcg ctttccctga gcgagtgctt gcggcaccca tctccgaggc tgggctggcg
     8461 ggcgtgagcg cgggtatggc gctgcgcggg ttgcgcccgg tgctggagat catgttcgga
     8521 gatttcatca cgctcaccgc ggaccaactt atcaaccaca tcagcaaatt taactggatg
     8581 tatgatcatt cagtctgttt accgatggtc atccgcgccc ctatgggggg ggggcgcggc
     8641 tacgggccga cgcattcaca aaccctggaa aaacttttca tcggcatacc gggattaacg
     8701 ctgattgcgc cgctgaacct gaccggtaaa ggcggtactc agcaaccggg tgaactgctg
     8761 gaggaagtta tcttgcatac cgaggaacct gtgctgttta tcgaaaacaa gctgcagtac
     8821 ctgctgcctg tctaccagcc gcaagacctc cctgaactgc actggttggt tgaacaaccc
     8881 gtgaagaaca cgttaatgaa aacccccacc tacctggtga aggtgagcgg cgcgcccgcg
     8941 gcgcgggcga gcctggcggc ttatgggtac atggcagagc tggcccgcca ggcgatgctg
     9001 aaactggctt acgaggatga gatctttgtg gaactggtca ttcctacccg cctggctcct
     9061 tttgatttca ccgcgctggc ggattcgata aaacgcaccg gacgcctggt gaccgtggaa
     9121 gagggaaacc tgaccggcgg ttggggggca gaggttatgg cgcgggcact ggaagaggtc
     9181 ggcggaagat tgcgagcggc agtgcgcgtt gccgcgcgcg acctgccagt ggccgccgcg
     9241 ccttccttag aaaacgagat cctgcctgat gtagaagata ttgttcaggc tgtaagaaag
     9301 gtggtgggtc gaaatgagtg aacccctgca aatactggtg cctttgatca accccaatga
     9361 gcctgaagcg ctgctggcag aactgcgggt gcgcgagggc gacaaggtga ccgcgggtca
     9421 ggtgctggcc agctttgaaa cgaccaaatc aacatacgag ctgaccgcgg aacgggatgg
     9481 ttttatcctg aacctgcgct tcaaggctgg agatacactg cgttcaggtg aacggttatg
     9541 ttacctggct gattcaatcg ggcagccgct ccctcatgaa accgccaaac ctgccgcaga
     9601 caggctgccg cgacaggagg agttacctca gggattgcgc atcacccaac ctgccctcgc
     9661 cctggcgaaa accaaccagg ttgacctgga agatttaccc cggggcgtgc tggttactga
     9721 agaaatggtc agaaaaatgc ggcccatccc ggtcgaggat gtggacccgt ccgcgttgat
     9781 catttatggc ggcggtgggc acgcgaaatc gctcatcgat ctcatccgct tgttggggca
     9841 gtaccgcatc cacggcattc tggatgacgg catcgccgcg ggtacacgca tcctgggcat
     9901 tgaggtattg ggaggagcag aactgcttct tgggctgcga cgccaggggc tggggaaggc
     9961 ggtgaacgcg gtgggtggga ttggcagcat tgtgccgcgg ctggcggtgt atgaaaaact
    10021 ggcggcggct ggtttcgagt gccttaccgt agtgcatccg cgcgcatttg ttgaacccaa
    10081 cgcccgcatc gctgaaggct gccaggtgtt tttcaacgct tatgtgggca gcgaggtgga
    10141 ggttgggttt ggcagcatca ttaatacagc cgcggtcatc tcgcatgatt gccgcttggg
    10201 taaaaccgtc aatgtttccc cgggggctat gctggcgggg gcggtgacgc tgggagaccg
    10261 cgcgcttatc ggcatgggcg ccaccatcaa cctgggggtc accattggcg cgggcgcgcg
    10321 cgtgggcaac agtgcggtgg tgaaagcaga tgtgccggag aacggcatcg tgcgcgccgg
    10381 cgcgacctgg ccggcggatt gaacagggca tgcgctttct tgattacggc ttgagaatgg
    10441 taaaatgaga gcgctcaatg tagttttccg tgaacgactc tttcccctgt tataacaggt
    10501 gaatccgcct gtggaggaac tggatgttta aaaaaccaac tcctgctacg caggaacaaa
    10561 cggcgccgat cgaacgggtt aattcagtgc tcggaccggg catcaattgg gctggcaacc
    10621 ttggcggcag ggggggtatc cgcattgagg gaacttttga gggcgagatc cgcattcagg
    10681 gtctggtggt ggtgggcgag accggtcgcg tgacctgcca gaacctgcgc gcgaacagcg
    10741 taattgtggc tggcgcgctg cgcggcaatg tcaccgctga gaagctcgag atccgctcca
    10801 ctggcagggt atggggagac gtggtgacca tcgctttcgc aactgaagaa ggcgcttttc
    10861 tgcgcggtca ggtgcgcatg gaagagaaag tggatctgca cctggaggaa ccctcagaag
    10921 cggtgaagga tctggacgag ccagcggatg agaagagaat ggatactccg aaaagacctg
    10981 ccaggggcag acaggtggcg ggagattgaa ctcaatggac gtgaagttct ggctgggtta
    11041 cctgagcgag tggctgggcg tgatcgcggt agtgatgatc gccgggacca gcccgctttt
    11101 gcgaaaaatc aggcgcatcg agttccgctt ttccaggagg gaagcggcct ttgcgctgac
    11161 tcttttcgcg cttatcttct tctttgcctt tcaagtttat gataataatc tgcttggctt
    11221 ccttaaaaca gccgcgggag ccttcgtggg cggtgatgtc gcgttacgta tgctcatagc
    11281 tgtcatcgcg ttgctgccgg ctatcattct cctccttgcg cgcggtcaac ctctcaagag
    11341 caccgggtgg agcaaagaaa acacccgcgc cagcctgatg ctggggttgc tcctggcggt
    11401 gctgaccatc tttctgcgtg gcaagttcac ccctttgctc aaggggttca ccgaagaaaa
    11461 gggcagcctt ttgctggtgc tgctcatact gagcctggcg gaagagagca tctttcgtgg
    11521 gtacatccag ttgcgcctga tgtcttacct cgggaccacc tggggctggc tggcgactgc
    11581 ctttctctac ctcctgtggc agcttcccgg cagggcctgg ctcagccatt tccccactgc
    11641 ctggcctgag gtgctcatca cgctggcgca ggcgctgata ttgggttgga tcatgcgcaa
    11701 gagctaccat gtggccgcca cggccttgta ccgcgccgtg gctgcctggt taatggtcat
    11761 ttgagccttt ttaattacct ggaacaacgt atgcgcctct ccaacctttt cagccagacc
    11821 ctgagggaaa accccgcaga cgccgagttc gccagccatc aactgctggt gagagccggt
    11881 tttatccgtc agctggggag tggcattttc acctatatgc ctctggcgta tcgggtgctc
    11941 gaaagagtca agcgcatact gcgcgaggaa atggaggcta ttggcgggca ggaactgtta
    12001 atgcccgtcg tgcagccggc tgatatctgg aaagaatcca ggcgctatta tcaaattggc
    12061 agcgagatgg ggcgctttac agaccgctct ggtcacgaaa tggtgctggc gatgacccat
    12121 gaagaggcag tatccgacct ggtacggggg gttgtccact cttaccgcca gctccccgcg
    12181 atcatttatc accttcaaac caagtggcga gatgacccgc gtccgcgcgc cgggcttatc
    12241 cgcgtgcgcg aatttctcat gctggattca tacagcctcg acgcggattg gcaggggctg
    12301 gatacccgct acaagcaaca ttacgaagcg tattttcgca tcttcaggcg ctgcggcttg
    12361 ccggtggtgg cggtgcgctc tgatagcggc atgatgggcg gggaggaggc tcacgagtac
    12421 atgtacctca cgcctggtgg tgaagacagc ctgctgtttt gcgatgcttg cggttactcg
    12481 gcgaaccgcc aggcagccac cacgcggaaa atggagcccg ccaaagaagc ctttctgccc
    12541 ctggaaaagg ttgccacgcc gcgcgtttcc tcaatagatg atttgacggc gtttttgaat
    12601 atcccacccg cgcgcaccgc caaggccgtg ttctacatgg gactggtgga agaggacggc
    12661 ggagaacaga aatgggcgtt tatcttcacc gtggtgcgcg gcgacatgga agtaaatgag
    12721 accaaagtgg ccaacctggt gaaagccagc gatttacgcc cggcgactga agacgaaatc
    12781 cgttcgattg gcgcggttcc aggttacgcc tctccacttg ggctgaaggg cgtgacggtt
    12841 tttgtggatg agctggcggc gaaatcagcc aacctggtgg ccggagccaa tgaagaggga
    12901 taccacctgc gcaacgtcaa ccatgggcgc gactaccagg cgagtatgat cgcggatatc
    12961 agcgccgcgc gcgagggcga tgcctgcccc gaatgcggag cggcgctgcg gctctcgcgc
    13021 ggggtggagg tggggaacat cttcaagtta ggcgcgcgtt attcagacgc gctgggatgc
    13081 acttatttag ataaaaatgg acagagccag ccggtgatca tgggttcgta cggcattggc
    13141 ctgggcaggc tcatcgcctg tgttgccgag gaacatcacg acgagcgcgg gctggcctgg
    13201 ccggtcagcc tggcgccttt cccggtgcac gtggtggtgc tcaagggaaa agaaatggac
    13261 agcgaagcgt tggcagcgcg tgttgagaac gcgctgcgcg acgcggggct cgagccgctg
    13321 gtggacgacc gcgaggaatc agcgggagtg aaattcaacg acgcggacct gatcgggctg
    13381 ccgctgcgga tcacagtaag tgaacgttcg ttcagagtcg gtggactgga aataaagccc
    13441 cgccgcggcg gtgaaacggt tgtcgtgcca atggagaacc ttgtcgttga agtgcaacga
    13501 ctcctgaacg cgctggagga accactttga acccgcacaa acgcgccgcg aagagaggat
    13561 cattcacacc atgaaaaatg ttgaacctgt tgtacccatg tccttacccg acctcagcga
    13621 tgcggaacgg gaggcggtta tggaagtgtt gcgcacgccc catctgagca tgggtccgcg
    13681 catcgacgat tttgaagccg ccctggctga atttgtcggt gcgaaggagg cgattggcgt
    13741 cagttcgggc accgccgggt tgcacctgtg cgtgcgcgcc gcaggtattc agcccggaga
    13801 cctggtgctc accacacctt tttcattcgt tgcttcgacc aacgccatcc tctttgaagg
    13861 cggcattcct gtttatgtgg atgtggaccc caagaccggc aacatggacc cgctgctggc
    13921 tgcgcaggcg ctggaagacc tgcgaaaggg cggtgccgcg gcgcggcgct ggttgccgcg
    13981 caaaggagca ggcgacactg ccaggcttaa agcgctgctg ccggtggatg tgtttggaca
    14041 acccgcggat tatgacctgc ttcagccgct ggcggaagaa tttcatctga agcacatcga
    14101 ggattcctgt gaatcactgg gcgcggagta taaatccagg cgcttgggat tgatgggaga
    14161 ttacggtgtt ttcgcttttt acccgaacaa acaaatgacc accggggagg gcggtatcat
    14221 cgttacggat gatgccgacg cggccaggta catgcgcgcg ctgcgcaacc aggggcgcgc
    14281 gcccggtgat acctggctgc agcataccta cctgggttac aactaccgca tggatgagat
    14341 gagcgccgcg ctggggctgg cgcagctgcg ccgcattgaa gaattgctga acaaacgcca
    14401 gcaggtggcc gattggtatg ccagccgcct ggcgaacatc gatggcgtgc agataccctt
    14461 aatcgcgcct gagaccacac gcgtatcctg gttcgtctac gtcatccgcg tggcccccgg
    14521 gcttgaccgt gatgcccttg ccgagaaact gcagctcagg ggtataccgg tccgccctta
    14581 ctttctcccc atccacctgc agccttacat ggtggataag ttcgcctacc agttgggaga
    14641 ttaccccgtg acggaagacc ttggaaggcg cgggctggcg ctgcctttct caggtaagat
    14701 gacagaagaa caggttgacc tggtttgccg ggtattggcg cagtgcgtta caaaataagg
    14761 ttggaggtgg aatgagccag cgccaggcgt tttacccgcc caagaaagcc ggtattttgc
    14821 tgcacgcgtt cctatcgctg cttctgctgg ggggcatagc gtttctcctc gtgctggcat
    14881 tttcacaacc gccgagctgg aggctggtct tttacctgct gggagttatt ttactgggta
    14941 tgctgctgcc gctggcggct taccgcggtt acgccctgtt aaaagcggat tacagccttg
    15001 agcgcgatgg gttacaggtg cgctgggggc tgcgctcaga agacctgccg gtaaccgatg
    15061 tgttatgggt gcgcccggca tctgacctgg taaaaccgct gactctgcca aggttcgcgc
    15121 tgcccggcgc catactgggc acatctcacc acgaagagct gggggaggtg gaatttatcg
    15181 ccagcagcgc gaggcatttg gtgatcgttg cggcgctgga aaagacattg attctttctc
    15241 ccgctgaccc tgaggatttc tcccgccgct ttcagcgggt gattgaaatg ggaagtcttt
    15301 cgcccctgcc agcgcacacc accattccag ccgcgtacat gaaccaggtc ttcaaagatc
    15361 cgctggcaaa agtgctgatg gtgctgagcg ctgtcctcac gctgttgctg ctgttggcaa
    15421 ccggtctcct catcccgtcc cgcgaggtgg tatccatcgg ctatgacttc aacaccctgc
    15481 cgatgccccc ggttccatcc aaccgcctgt tgatgctgcc ggtgatcgcc ctgttctttg
    15541 ttctgttgga ctttatagcc ggtctttttt tgtaccgccg cgcagaaacc agagccatca
    15601 gttacctcat ctgggcggct ggcatcctct ccccgctgct gctgttgatc gcggtgttgc
    15661 tgaccaccat ggtacgggtg tagcatgggg gttcctttat ccccgattca attgattttt
    15721 ggtgtgctgc tggctgcgct ggtggtgtac gcggcttacc gcgcgcgctc gctggaccgc
    15781 agcggggcgc tggcggcttt tttactgggc gtggtcgtgt tcggcattgg cgggttctgc
    15841 tgggcgctgg tgctgctcac tttcttcatc acctccagcg ggctgtctca cctgttcagg
    15901 gtgaagaaac agaaggtgga ggaacaggcg gtcaagggcg gacgccgcga cgcctggcag
    15961 gtaggggcaa acggcggggt tgcgggactg gcggcgctgc tctccctggt catgcctgga
    16021 tcggttttac catggctggc gttcgctgcg gcgttcgcgg cagccaatgc ggatacctgg
    16081 gcgactgaac tgggggtgct caatcgctcc tggccgcgca ggctgaccga ctggaagcgg
    16141 gtacctccgg gcacgtcagg cgccgtgagc ctggcgggca cgctggctgc cacaggcggc
    16201 gcgctgctcg ttgcgttgat ggcggcggcg tgttggcctg tggtcaacac aagttttcgg
    16261 gaaaaactgg tgtgggggct ggtcatcttg ctcgctggcg tgtttggcag cctggtggac
    16321 agcctgttag gcgcgaccct tcaggtgatt tactggtgcc cggcgtgcga aaaagagacc
    16381 gaaaaacacc cgctgcacag ttgtgggaaa ccaacccgcc gcgtcagggg gtggggctgg
    16441 ctgaataatg atgtggtaaa cctggcctgt accctcagcg ctgcgctgct ggcgtgcctt
    16501 ttggggctgc tcctccgtta acgccctact ttacaaatat gcaataaaat attcctttct
    16561 tatgcatggt tgtataataa tcctgcggat atgatttaaa aggaatattt gtgattcaga
    16621 aatggcgatc atctgaatga attttatgct tctatggcta cgtagcaagg atagaagaat
    16681 agcgaaacca gctcaggggc ttgttgaatt tgccctgatt atcccccttt ttttaatgct
    16741 gttatatggc ttgttcgaag tcggtcgggc ggtttttatg ttaagcgccg tacgcaatgc
    16801 cagccgtgat gccgttcgct atgcgggggc aagcggtacg aacccgagtg gtgttgaacg
    16861 ataccgggat tgcgcaggaa ttcgcgatag agcaaaacgg gtgagtgcct ttgtggattt
    16921 gagcgcggtg aatgccgtag aaatcagtta cgacaaagga ccatccacac cccccatctc
    16981 ttgtgaagcc ttggtatcca gcggtaaaaa actggcgttg ggcgatcggg ttgttgttac
    17041 cgtcagggct acctttgtac cttttgttgg tcctctttta aattttaata caat
//
LOCUS       DAOTLW010000038        16661 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_3869_length_16661_cov_9.974286, whole genome shotgun sequence.
ACCESSION   DAOTLW010000038 DAOTLW010000000
VERSION     DAOTLW010000038.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16661)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16661
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3869_length_16661_cov_9.974286"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="P5516_06775"
     CDS             complement(<1..641)
                     /locus_tag="P5516_06775"
                     /inference="COORDINATES: protein motif:HMM:NF025104.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92174.1"
                     /translation="MRNRYMVMIDMALTIIAVLGAYALRLELGALFFFYLPSAYWMIA
                     VSLLIKIPIYYLFSLYRRLWGYASTRELRLIFLAVTSASVLVSAVMILLLTFKVFNGF
                     PRSVLIIDWLLSIFLVGGTRFAIRLIADNVSTAANSMKKRPAKRALIIGAGDAGALVV
                     RELQKNPDLNLNPVGFLDDNPAKLKQQIHGIPVVGSLADIGKIIEHRKIEEVI"
     gene            complement(659..2080)
                     /locus_tag="P5516_06780"
     CDS             complement(659..2080)
                     /locus_tag="P5516_06780"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HRT92175.1"
                     /translation="MTVEKPQIKKRWRIRNGERQAILFLGDMLIAYLAVFVGLYFWGQ
                     KDWLDFSWAFLGQRAPFWFYLLPFLWVIFLIEVYDIRKANHRKDVIRGVATAAGISFI
                     IYLVIFFISDPNSLPRRGVFIFIIAATILTFAWRSLYIRIFTAPVFMRRALMIGAGKA
                     GSTLASMLREIHPGPFILVGYIDDDPEKQGEEISGYPVLGTAERMTAVIGEQDITDLI
                     FAISGELKPELFRAILNAAESDIEVTSLPKLYEEVFGRVPIFLLQTDWILRSFIDQTH
                     TSGVYESVKRLIDIAGSIVGLLLLVLLYPLIALSIIIDSGFPILYSQMRVGKNGHPYK
                     IFKFRTMYQDAEKDGVARTASANDARITRFGKILRRSHVDELPQCWNILRGENSIIGP
                     RAEQIELVNKFQQELPFYRARLFVRPGLTGWAQINQRYATTVEDTAVKLEYDLYYIKN
                     RNLLLDLTIILRTVGMVLGFKGL"
     gene            complement(2070..3086)
                     /locus_tag="P5516_06785"
     CDS             complement(2070..3086)
                     /locus_tag="P5516_06785"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT92176.1"
                     /translation="MEPVKVSVIIPCYNEEATIEELLSALLAQTYPIDLMEVIIADGM
                     STDRTREKITAFKAGHPQLTIRVVDNHQHTIPAALNQAIQASTGAVLSRMDAHAIPAP
                     DYIEKCLRALNAGLGENVGGVINIRAGSDTWVGRAIAVATAHPLGVGDAHYRTATKAG
                     VTDTVAFGTYRRDVVKSIGGYDESLRINEDYEFNTRVRRSGGKIWVDPAIRVDYYSRP
                     DLRSLARQYFSYGYWKYKMLRRHPDSLRWRQALPPVFVSGILMLSLLSTFWIPARILL
                     AGVASLYLAILVAGTIQPAIQQKRSSLIPGVPLAIMTMHFSWGSGFLWSMLKSLVKGS
                     EHDR"
     gene            complement(3117..3527)
                     /locus_tag="P5516_06790"
     CDS             complement(3117..3527)
                     /locus_tag="P5516_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HRT92177.1"
                     /translation="MACTDKKVYQSSEAPAAIGPYSVAAGGGPFVFSAGQIGIDPETG
                     NLVPGGIEEQTHQVFSNLSAVLRSASSCLEHVVKTTVFLRDMKDFPAMNKVYGGYFPS
                     NPPARTTIAVAGLPKDALIEIEVVALLCKDGDCA"
     assembly_gap    3618..3717
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3741..4814
                     /gene="prfA"
                     /locus_tag="P5516_06795"
     CDS             3741..4814
                     /gene="prfA"
                     /locus_tag="P5516_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HRT92178.1"
                     /translation="MLEKITAIENRYNELTRLMDENIQDYQKVVELAKERSELEPVIQ
                     LSARYRSLLNQLEQAKELESSGDDEMRALANLEIEALPPQIEELEKQLKRLLIPRDPR
                     DDRNVFIEIRAGAGGDEAGLFAAELMRMYMHYAEARGWNVEMISSNETGIGGFKEVIF
                     MVKGKGAYSRFKFESGVHRVQRIPATESSGRIHTSTVTVAVMAEVEEVDVQIPESDIT
                     IDVFRSSGAGGQNVQKNATAVRITHIPTGIVVACQDERSQLQNKLRAMGILRARLFEI
                     EEEKRRQERDENRRSQVGTGDRSEKIRTYNYPQSRVTDHRINLSSYNLPAVMTGEIDL
                     FIDELALRDETDRLSSSGDYDES"
     gene            4801..5646
                     /gene="prmC"
                     /locus_tag="P5516_06800"
     CDS             4801..5646
                     /gene="prmC"
                     /locus_tag="P5516_06800"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HRT92179.1"
                     /translation="MTRVNLGRWIAQKRAQLQGKTDFPSIEINAIASHLLQQPTAWVI
                     SHPETVLTDAQYQLMEDAVNRLLLGEPLAYITGHKSFFGLDFIVDNRVLIPRPETEIL
                     VEHALAWLKAYPGKRKVIDIGTGSGAISITLVKNLAGLQVTAVDISAAALAVATANVH
                     YHKVANQIRLIESDLFEQVDQKFHLVLANLPYIPSIDLNSLSVVKYEPREALDGGSDG
                     TELITRLIDTMPHHLLPGGCAILEIQYNQDNLVSSIAARRFPDASISFIKDLASINRF
                     LKIQL"
     gene            5660..6283
                     /locus_tag="P5516_06805"
     CDS             5660..6283
                     /locus_tag="P5516_06805"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HRT92180.1"
                     /translation="MQTITLPADNPASIETALDLLTEGEIVAFPTDTVYGLGANAFYS
                     PGIIKLFEAKGRDANKAIAVLIGDNGHLDLLTDFLSANARKLIKHFWPGGLTIVVPKK
                     KDLPELLSAGSSIGIRMPNHNIALELLNKFGPLATTSANLSGKNNPHNAGDVLNQLNG
                     RVPLILDGGRCPGGVPSTVVDCSTDEVRILRPGAISHEAIDAVISRL"
     gene            complement(6308..8044)
                     /gene="cydC"
                     /locus_tag="P5516_06810"
     CDS             complement(6308..8044)
                     /gene="cydC"
                     /locus_tag="P5516_06810"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydC"
                     /protein_id="HRT92181.1"
                     /translation="MKGLWRVCIILKEFSGQVFLSLILGVAAVAAGIGLLGTSAFLIA
                     SAAFQPSIAELQVAIVGVRFFGISRGVFRYLERLVSHSVNLHVLTRLREDFYKRVEPG
                     APANLFSQQGGDMLQRVMGDLETLENFYIRTIAPVVIALVILAGVSLFIGGYALQLGL
                     IMAAGMGISGFIHPALAVFISRSPIIQLSRSKAAASARLVETLQGLEDIQVYNAQAPY
                     LEALLGEFRWSAVLQKRLVNLNAVSSGLALLFTHLTVLLLLWVGIPLVSAGQFSGISL
                     AVITLVALASFEAVSAMPAAALHLNASAAAANRQLTIGEEMSVYPRLTKTPRNIQRPE
                     LAFEAVRFTYPNAQRPTLEDIHFKITSGCKTALVGASGAGKTSLVNLLLNFEQPQEGS
                     IQLNGVKLAEMDAEESRSWFAVLPQSVYLFNDSLRGNLALAKADASDEELISALRAAC
                     LSAWYESLPEGLDTWVGEHGVKMSGGERQRLAMARVLLQDRAFIILDEPTANLDPATA
                     RKMMDQLFTRSPNKGLLLITYDLGLLREMDEILVLSEGTITQRGRMDSLLKAGGEFKH
                     IFEMENNKIRDS"
     gene            complement(8041..9783)
                     /gene="cydD"
                     /locus_tag="P5516_06815"
     CDS             complement(8041..9783)
                     /gene="cydD"
                     /locus_tag="P5516_06815"
                     /inference="COORDINATES: protein motif:HMM:TIGR02857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HRT92182.1"
                     /translation="MRIHPRLFKEARKNGLLFLLTALASFLAGGIAVIQSYQLSRVIS
                     GVLIDQRTLKEVSSILLFILLLIFSRVVLTIISEALAGRMTIAIKQRLRAELLDKVNR
                     LGPEFLKNEKTGELTTTALQGVDALDAYFSQYLPQVLISVALPLTILAVVFPMDLLTG
                     IIFVFTLPLIPVFMVLIGWASEALTKRQWKALTYLGNYFLDTLQGIATLKMLGRSKER
                     GNEVRDASERYRLATLAVLRVTFISALALELIATISTAVVAVEIGLRLLYGRIAFQQA
                     FFILLIAPEYYLPLRNLSARYHAGMTGVTAAQKIFEILDVPEPARQPVLSIENLEQVF
                     RTDYRLRVSNLYYSYPGMGSHSIQDISFCMQKGLHYALAGRSGSGKSTLARILLGLIE
                     PQEGQIWVNGSQVQAYSLEDWRRFVGWLPQTPFIFNDTLFNNVTLRDTRFSTSDVERA
                     LRLAGLAQLLATLPLGLHTPLLEGGARFSGGQLQRVALARIFLRQPSLLVLDEPTSHL
                     DPGLARALDDTFKTLLQDRTTLTIAHRLSTIAQADEVIFLKDGQIGAIGKHATLLETC
                     PDYGAFIHGSGVLA"
     gene            complement(9793..10482)
                     /locus_tag="P5516_06820"
     CDS             complement(9793..10482)
                     /locus_tag="P5516_06820"
                     /inference="COORDINATES: protein motif:HMM:NF014670.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HRT92183.1"
                     /translation="MLLGEMTWMEVEQYLKRDDRIMLVTGATEQHGYICLETDIKIPL
                     TLASHASERTGVLVAPPLNFGCSSYFVDFPGTISFRVQTLINAVEDMVRSVHHQGFKR
                     ILFVNGHGGNDPAKLALGELVNELPDLQVSWYDWWKSSSVAAVAEAHGLGMYHASWME
                     AFPFLRRSGMPDGIKEAFKPPQILPSKKVRAALVDGVYGGAFQASDEIMNEVFNAALQ
                     DVLGLLEFKMS"
     gene            complement(10604..>11678)
                     /locus_tag="P5516_06825"
     CDS             complement(10604..>11678)
                     /locus_tag="P5516_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018287220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRT92184.1"
                     /translation="LPDDVEWKPTGESPLKLHPTWRFTTCPQCGKPAERETDTMDTFV
                     CSSWYHLRYLSPDYDKGPFDPQEYDYWMPVDIYTGGIEHANMHLIYTRFFHKALRDMG
                     LTKGNEPMLQLRNQGMVLGEDSEKMSKSRGNVVSPDDLVHTYGADATRAYLMFFARWD
                     MGGPWSSGGIDGVMRWLRRAWSLVLEPGQTGAVNEESVKTLRRKVHQTLRSVSRDFEQ
                     FEFNTIISSLMELLNEMAHLKDSVYGSDAWKEAIEIYLQMMAPVVPHIAEELWQRIHK
                     PYSVHTSRWPSVDEKAAADEEITLVVQLNGKVRDRITVPVDISEEEAKTRALKSDIIQ
                     KQMEGKEARQIIYVKGRLINIVV"
     assembly_gap    11679..11778
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<11779..12803)
                     /locus_tag="P5516_06830"
     CDS             complement(<11779..12803)
                     /locus_tag="P5516_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRT92185.1"
                     /translation="MARQVPEYIPAEIEPRWQRQWEAAGLYHADIDKKRPKFYALTML
                     PYPSGDLHIGHWYAISPSDARARYKRMNGFNVMFPMGFDAFGLPAENAAIKHNIHPRK
                     WTYANIQKMREQFRSMGTMIDWRREIVSSDEKYYRWTQWFFIQLVKHNLAYQKLSPVD
                     WCPHCNTTLAREQVWGDDRHCERCGTPVIKKDLKQWFFKTTRYADELVNYDGLDWPER
                     VRTLQTNWIGRSEGALVTFKTEAGDALEVFTTRPDTLWGVTFMVLAPEHPLVEKLTTI
                     EQRAEVDQYVLDASRQSDIQRESTEKEKSGIFTGGYAINPVNGEKIPVWISDYVLMTY
                     GTGAIMAV"
     gene            complement(13078..13150)
                     /locus_tag="P5516_06835"
     tRNA            complement(13078..13150)
                     /locus_tag="P5516_06835"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13115..13117),aa:Ala,seq:ggc)
     gene            complement(13189..13261)
                     /locus_tag="P5516_06840"
     tRNA            complement(13189..13261)
                     /locus_tag="P5516_06840"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(13226..13228),aa:Ala,seq:cgc)
     assembly_gap    13530..13629
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <13630..14296
                     /locus_tag="P5516_06845"
     CDS             <13630..14296
                     /locus_tag="P5516_06845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRT92186.1"
                     /translation="SEMRRAVQIAQRSLLDILPLVKPGVNEKTIAAELTIQLLRNGSD
                     SELPFQPIVAGGPNSANPHLVPTERPLQSGDLLVIDWGARSNGYCADLTRTFAIGNID
                     PELKAIYEAVKQANSTGRAASKPGATAGEVDLAARAVIENAGYGAFFTHRTGHGLGLE
                     DHEPPYIFNGNSLALRPGMTYTVEPGIYLPGRGGVRIEDNLVITHTGCETFSDLSREL
                     MIL"
     gene            14317..15081
                     /locus_tag="P5516_06850"
     CDS             14317..15081
                     /locus_tag="P5516_06850"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HRT92187.1"
                     /translation="MAKTLTMATAEPFFIPAGKTGCLLIHGFTGTPKEMRMLADALAL
                     EGYTVLAPRLFGHATNPRDMSRARADDWIASVEDGLNLLKGCTDTQVVMGLSMGGVLA
                     LIAAARFDCRAAVTFSAPCSLPHDPRAWLLPLIGRLNLPIKKGKPDWRDLAAAQEHKD
                     YPYYPSRAIIELDRLIKLMQGELENIRVPVMLVQSRADRGIPPESLETIYNRLPTAEK
                     TSFWVEDSGHVIICEPERERIFSQVGAFLKQVLKDA"
     gene            15078..16226
                     /locus_tag="P5516_06855"
     CDS             15078..16226
                     /locus_tag="P5516_06855"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92188.1"
                     /translation="MNRNLLLVTISLFAWGMGEGLFIYFQPLYLQEFGADPVMIGSIF
                     GVMGVAMAVFQIPSGYLSDRFGSRSIMWASWFLGTSAAWTMALANTLPTFVVGLILYG
                     LTSFVVAPMNSYITKVRGDWSVGRALTFPSGFYHLGAVLGPISGGLIADRLGLKTVYM
                     VAAIIFIISTIIVLFTSQAPETHHADQVDATTKGTLKNPRFFAFLVITLLSIFAIYLP
                     QPFTPSFLQNQQAFSRSMIGILGAVGSLGNAVATLALGSLPSVIGFIIGQVWVLVFSL
                     TFLFARVPFWFGFGYFFIGGYRLCRSMVLAISRTLVNPRQTGLAYGMVETMNAIAVII
                     APVIAGVLYKNNPLSIFSVSLVIISITIILNLTVLVRQKRGRLHEPEN"
     gene            16210..>16661
                     /locus_tag="P5516_06860"
     CDS             16210..>16661
                     /locus_tag="P5516_06860"
                     /inference="COORDINATES: protein motif:HMM:NF012955.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT92189.1"
                     /translation="MSLKIDTFTSSMMSNNVVILHDDETRHAIIMDPSFEPEQVLTFI
                     NNNSLSVEMILCTHGHFDHFAGLAYLLTKLHPKPKVGLHESDLALWRAGGGAVHFRIP
                     IAVPEDPDIFLTENQTIHLGDGQIQIRHTPGHSPGSVVFYMESLHTAVV"
BASE COUNT         3937 a         4494 c         4062 g         3868 t
ORIGIN      
        1 atgatgactt cctcgatctt acgatgttca attatcttcc cgatatcggc gagtgaccca
       61 acaaccggta tgccatggat ttgctgcttt agtttcgcag ggttgtcatc taaaaaccct
      121 accgggttga gattgagatc agggtttttc tgcagttcac gcaccaccag cgcgccggcg
      181 tctcccgcgc caatgatcaa cgcccgcttg gcaggccttt ttttcataga attggctgcc
      241 gtgctgacgt tatccgcgat aagacggatg gcaaagcgtg taccgccgac aagaaatatc
      301 gaaagcagcc agtcgatgat cagtaccgaa cgtggaaacc cgttaaaaac tttgaaggtc
      361 aacagcaaaa tcatcactgc tgaaaccagc acagaggcgc tggttacagc aagaaagatc
      421 aagcgtaatt ccctggtgct ggcgtatccc cacaaacgcc ggtataggct gaacaaataa
      481 tagatcggta tcttaatcaa caaagaaacc gcgatcatcc agtaagcaga aggcaggtag
      541 aaaaagaaaa gcgctccaag ttccagccgc aacgcataag cgcctaacac cgcgataatg
      601 gtaagcgcca tatcgatcat gaccatatat ctgttgcgca tgttgaattt tattctcatt
      661 aaagtccttt gaatcccaga accattccaa ccgttctgag gatgatggtc aaatcaagta
      721 ataggtttcg gtttttgata tagtatagat cgtattccag tttgaccgcg gtatcttcga
      781 ccgttgtggc ataacgctgg ttgatttgcg cccagccggt gagcccgggt cggacaaata
      841 agcgcgcgcg gtagaagggg agttcctgtt gaaatttgtt gaccagttcg atctgctctg
      901 cgcgcggacc aataatactg ttctcacccc ggaggatatt ccagcattga ggtaactcat
      961 ccacgtggct gcggcgcagg atctttccaa agcgggtaat acgggcatca ttggctgaag
     1021 ctgttcgcgc cacgccatcc ttttctgcgt cctggtacat ggtacgaaac ttaaatatct
     1081 tatagggatg cccatttttc ccgacgcgca tctgggagta gagtatggga aacccggaat
     1141 caatgatgat agaaagcgca atcagggggt acagcaaaac cagcaggagc aaaccaacaa
     1201 tactgcctgc gatgtcgatc aggcgcttga cgctttcata tacgccgctg gtgtgcgttt
     1261 ggtcaataaa agaacgtaat atccaatcgg tttgcagtaa aaatatcggc accctgccaa
     1321 aaacttcctc gtaaagcttc ggcagcgaag taacctcaat atcgctctca gccgcgttca
     1381 aaatagcgcg aaacaattca ggtttcaact cccccgagat ggcaaatatc aggtcggtga
     1441 tatcctgttc accgatcaca gccgtcatcc tttccgctgt ccccagcacg gggtaaccgc
     1501 tgatttcttc cccctgtttt tcaggatcat catcgatata accaacgagg atgaacggcc
     1561 ccgggtggat ttcacgcagc atggaagcca gtgttgaacc agccttgcca gcgccaatca
     1621 tcaaggcgcg gcgcatgaaa accggggcag tgaaaattcg aatgtaaagc gaacgccagg
     1681 cgaaagtgag aatggttgcg gcgataataa agataaagac gccccgccgg ggtagcgagt
     1741 ttggatcaga aatgaaaaag atgaccaggt aaatgataaa actgatcccg gctgcggtgg
     1801 ctacccctcg aatcacatcc ttccggtggt tggctttgcg gatatcgtaa acttcgatca
     1861 agaaaatgac ccagagaaac ggcagcaggt agaaccagaa cggagcgcgt tgccccagga
     1921 aggcccatga gaagtcgagc caatcttttt gcccccagaa gtacaaccct acaaaaaccg
     1981 cgaggtaagc tatcagcata tcgccaagaa acaggatcgc ctgtctctcg ccattgcgga
     2041 tgcgccagcg tttttttatt tgaggctttt caacggtcat gctcagagcc tttaaccaat
     2101 gacttcaaca tactccacaa aaaaccgctt ccccaggaaa aatgcatggt cataatggca
     2161 agcggtaccc ctggaatcag gctggatctt ttttgctgga tggcaggctg gatcgtgcca
     2221 gcaacaagaa tcgccaggta gaggcttgcc actcctgcca ataaaatccg cgcgggtatc
     2281 cagaatgtgg ataataatga taacatcaaa atacctgaaa caaaaaccgg gggcagcgcc
     2341 tgccgccagc gtagcgaatc gggatgacgc ctcaacattt tatacttcca gtaaccatag
     2401 gagaagtatt gcctggccag cgagcgcagg tccggcctgg aatagtagtc taccctaatc
     2461 gccggatcaa cccatatttt cccgcccgag cgcctgacgc gcgtgttaaa ttcataatct
     2521 tcattgatac gcagggattc atcgtagccc ccaatgcttt tcaccacgtc tcgcctgtat
     2581 gtgccaaatg caaccgtgtc ggttacaccc gctttcgtcg ccgtgcggta atgagcatca
     2641 cctaccccca gtgggtgcgc ggtcgccaca gcgatggcgc gcccaaccca ggtgtcgctt
     2701 ccagcgcgaa tattaatcac ccctcccaca ttctccccca ggccagcgtt taaagccctg
     2761 aggcattttt cgatgtaatc cggcgctggt atggcgtgcg cgtccatacg gcttaacacc
     2821 gcccccgtag aagcctgtat tgcctggtta agtgccgccg ggatggtgtg ctggtggtta
     2881 tccaccactc ttatggtcag ttgcggatgc ccggctttga aggcggttat cttctcgcgg
     2941 gtgcggtctg ttgacattcc atccgcgatg atgacctcca taaggtcaat tgggtaagtt
     3001 tgagcgagga gcgcgctgag cagttcctcg atcgtcgctt cctcgttata gcaagggatg
     3061 atgaccgata ctttaaccgg ttccatcggt gggtcaaaaa tcctattctc agcctgttat
     3121 gcgcaatcgc cgtctttaca caacagcgcc accacctcaa tttcgataag cgcatctttg
     3181 ggtaaacccg cgaccgctat cgtggtgcgc gccggcgggt tggaagggaa ataaccgccg
     3241 tacactttgt tcatagcggg aaaatctttc atgtcgcgca ggaaaacagt ggtcttcaca
     3301 acatgttcca ggcaagaaga cgctgacctt aacacagcgg ataggttgga aaagacctga
     3361 tgcgtttgtt cttcgatacc gcctgggaca agattgcccg tttcagggtc gatgccgatt
     3421 tggcctgcgc tgaacacaaa tgggccgcca ccggccgcaa cggaatatgg gccgatagcc
     3481 gccggcgctt cgctgctctg gtataccttc ttatcagtac atgccatcat tacctcctgt
     3541 gatttttcta cctcctcatt ttaagcgaac tgtaagaaaa ggtaaatgca agatctcaaa
     3601 ggcgttttgc gcggttgnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3661 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngat
     3721 tctaattatt caggaatgcc atgctcgaaa aaatcacggc tatcgaaaac cgctataacg
     3781 aactgacccg cttgatggat gagaatatcc aggattacca aaaagtggtg gaactcgcca
     3841 aagaacgttc agaactggag ccggtcatcc agctttcagc ccgctaccgc tctctgctca
     3901 atcaattgga acaggccaaa gaattggaat caagcggcga tgatgaaatg cgcgcgctcg
     3961 ccaatctcga aattgaagcg cttcctcctc agattgaaga gctggaaaaa cagctgaaac
     4021 gcctgctcat tcctcgcgac ccgcgcgatg atcgcaacgt gttcatcgaa atccgcgccg
     4081 gcgccggcgg tgacgaagcg gggttgtttg ccgcagagtt gatgcgcatg tacatgcatt
     4141 acgccgaagc ccgcggctgg aacgtggaaa tgatttcttc caacgaaacg ggcattggcg
     4201 gattcaaaga agtgattttt atggtcaaag gcaaaggggc ttattcgcgt tttaaatttg
     4261 aatccggcgt gcaccgcgtt caacgcattc ccgccaccga atccagcggt cgtatccaca
     4321 cttcaacggt aacggtcgcg gtcatggcag aagtagaaga agtagatgtt cagatccctg
     4381 aatccgacat caccattgat gtatttcggt cttccggcgc gggggggcaa aacgtgcaaa
     4441 aaaacgccac cgcggtgcgt atcacgcata tccctactgg tatcgttgtc gcctgccagg
     4501 atgaaaggtc tcagttgcag aacaagctca gggcgatggg cattcttcgc gccaggctgt
     4561 ttgaaattga agaggaaaaa cgccgccaag agcgcgatga aaaccgccgc tcccaggtag
     4621 gtaccggaga tcgttcagag aaaattcgaa cttacaatta cccgcaaagc cgggtgaccg
     4681 atcaccgcat caacctttct tcttataatt tacccgccgt gatgaccggc gaaattgacc
     4741 tcttcattga cgaactcgct ctccgcgatg agacagaccg cctctcttca agcggcgatt
     4801 atgacgagag ttaatctggg ccgctggatc gcccaaaaac gagcgcagtt gcagggaaaa
     4861 acggatttcc cttccatcga aatcaacgcc attgcttctc atctcttaca acaacctact
     4921 gcatgggtta tttctcatcc tgaaacggtg ctcactgacg cccaatatca attaatggaa
     4981 gacgcggtca accgtctgct gttgggagaa ccccttgcgt acatcaccgg gcacaagtcc
     5041 ttttttgggc tggatttcat cgtcgacaac cgggtgctca ttccccggcc agaaacagaa
     5101 atactcgtgg agcacgccct tgcctggctg aaggcttatc ccgggaagag aaaagtgatc
     5161 gatatcggca ctggctcagg cgctatttcc atcaccctgg taaaaaatct ggctggtttg
     5221 caggttaccg cggtggatat ttccgctgct gcactggcgg ttgccaccgc caacgtccat
     5281 taccataaag tggcaaatca gatccgtttg attgaatcag acctgtttga acaggttgat
     5341 caaaaattcc acctggtgct ggccaacctt ccctatatcc catccattga tctcaattcg
     5401 ttaagcgtgg ttaagtacga gccgcgggaa gccctggatg gcggttctga tggaacggaa
     5461 ctcattacca gactgatcga tacaatgcca caccatctgt tgccaggtgg atgtgcaatt
     5521 ctggaaatac aatataatca agataacctg gtcagcagca tcgccgctcg tcgcttccct
     5581 gacgccagta tttccttcat caaggacttg gcttctatta atcgttttct taaaatccag
     5641 ctgtgaaaca ggttattcaa tgcagaccat aaccttaccc gcagataacc ccgcttccat
     5701 cgaaaccgct ctcgacctgt taacagaggg agaaatcgtg gctttcccta ccgatactgt
     5761 ttacggtctg ggagccaacg ccttctattc tccaggtatt atcaagttgt ttgaagccaa
     5821 ggggcgcgac gccaataaag ccattgctgt gcttatcgga gataacgggc atttggattt
     5881 gctcacagat ttcctttctg ccaacgcgcg taaattaatt aagcactttt ggccaggcgg
     5941 gctaaccatc gtggtaccaa aaaagaaaga cctcccagag ttactctctg cgggaagctc
     6001 aatcggtatc cggatgccca atcacaacat cgcgcttgag ctgctgaata aatttggtcc
     6061 tctcgctacc acttcagcca acctgagtgg gaagaataac ccgcataatg ccggagatgt
     6121 cttgaaccag ttgaacgggc gggtcccgct cattttggat ggcgggcgct gccccggggg
     6181 cgttccctcc accgttgtgg attgctctac agatgaggtc agaatccttc gacccggcgc
     6241 gatttcacac gaagccatcg acgcggtaat atcccgatta taaacctgca cagtttgcgg
     6301 ttggcactca cgaatcgcga attttgttgt tttccatttc aaagatatgt ttaaattccc
     6361 cgcctgcttt cagtaatgaa tccatcctgc cgcgttgtgt gatggtaccc tcgctaagca
     6421 ccagtatttc atccatctcg cgcagcaatc ccaaatcata agtgatcagc agcagccctt
     6481 tgttgggcga cctggtaaaa agctgatcca tcatcttccg cgcagtagca gggtcgaggt
     6541 tggcggtggg ttcatcgagg atgatgaacg ctcgatcctg cagcagcacg cgagccatcg
     6601 ccagccgctg ccgttctcca ccgctcattt tcaccccatg ttctccaacc caggtgtcca
     6661 gcccttccgg cagcgactcg taccatgcag aaaggcatgc cgcccgcagc gcgctgatca
     6721 gctcttcatc gctcgcgtcc gcttttgcca gcgccaggtt gccgcgcagg gaatcattaa
     6781 atagatacac tgattgcggt aacaccgcga accaggacct gctctcttct gcatccattt
     6841 cagcaagctt gaccccattc agttggatgc tcccctcttg cggctgctca aagttaagca
     6901 ataaattcac caggctggtt ttccctgcac cactggcgcc aaccagggct gtcttgcagc
     6961 cgctcgttat cttaaaatga atgtcctcca aggtaggtcg ttgagcattc gggtaggtga
     7021 aacgcactgc ttcaaaagcc agttctggtc gctgtatgtt tcgcggtgtt ttagtgagcc
     7081 gcgggtagac gctcatttct tcgccaattg tcaattgccg gttggcggct gccgcgctgg
     7141 cattgagatg aagtgcagcc gcgggcatcg cggataccgc ttcaaagctg gctaacgcta
     7201 ccagtgtgat cactgcaagc gaaatgccag aaaattgccc ggcgctgacc aggggaatac
     7261 ccacccataa caataacaac acagtcagat gggtgaacag taacgccaac ccgctgctga
     7321 cagcattgag attcaccagg cgcttttgca gcaccgcaga ccatcgaaat tcacccagca
     7381 gcgcttcaag gtatggcgcc tgggcgttat aaacctggat gtcttccaat ccttgcaacg
     7441 tttcaaccaa cctggctgat gccgcggctt tcgacctgct cagctggatg atggggcttc
     7501 gtgaaataaa aaccgctaac gcggggtgaa taaacccgct gatacccatc ccagcagcca
     7561 tgatcaaacc cagctgtaaa gcataaccac cgatgaaaag gctcacacca gcgaggatca
     7621 ccagagcgat cacaacaggg gcgatagtgc gaatataaaa attctccagc gtttccaggt
     7681 ctcccatcac ccgctgcagc atatccccgc cctgttgaga aaataaattt gccggcgcgc
     7741 cgggttcaac acgcttgtag aaatcctcgc gcaacctggt caacacatgc aggttgacag
     7801 aatgcgaaac caaccgttcc aggtagcgaa aaacgccgcg gctgataccg aagaagcgca
     7861 ctcccacgat tgccacctgt aattccgcga tcgaaggttg aaaggctgcg ctggcaatga
     7921 gaaaagcaga ggtacccaac aacccgatcc ccgcggcaac ggcagcaaca ccaagtatca
     7981 gcgagagaaa gacctgccct gagaattcct taaggatgat gcaaactcgc caaagccctt
     8041 tcacgccagt acccccgacc cgtgaataaa cgcgccataa tccgggcagg tttccagtaa
     8101 ggtggcgtgc tttccaatcg ctccaatctg cccgtcttta agaaagatca cttcatctgc
     8161 ttgcgcaatg gttgaaagcc tgtgcgcaat ggtcaatgtc gttcggtctt gcagcagagt
     8221 cttgaacgta tcatccagtg cacgcgccaa tccagggtca aggtgcgagg tgggctcatc
     8281 caacacaagc aatgagggtt ggcgtaagaa gatgcgcgcc aatgccacgc gctgaagctg
     8341 acctccgctg aagcgggccc ctccttccag caacggagta tgcaacccca gcggcagcgt
     8401 ggcaagcagt tgagctaagc cggccaatcg cagggctcgc tccacatcac tggtagaaaa
     8461 ccgggtgtct cttaaagtca cgttgttgaa cagcgtgtca ttgaagatga agggggtttg
     8521 aggtagccag ccaacgaacc tcctccagtc ttccagtgaa tacgcctgca cttgtgaccc
     8581 atttacccaa atttggcctt cctgaggctc aattaatccc aacaggatgc gcgctaaagt
     8641 gcttttccca gaaccgcttc gacccgccag ggcataatgc agcccttttt gcatacagaa
     8701 agaaatatcc tgaatggagt gagaacccat tcccgggtaa gaatagtaca ggttactgac
     8761 ccttagcctg taatctgtac gaaagacttg ctcaaggttc tctattgaaa gaaccggctg
     8821 gcgagcaggc tcaggaacat ccagaatttc aaatatcttt tgcgccgcag tcaccccggt
     8881 cattccagcg tggtatcggg cgctgaggtt gcgcagggga aggtagtact caggggcgat
     8941 caacaaaata aaaaaagcct gttggaatgc gatacgcccg taaagcagcc gtaacccgat
     9001 ctccactgcc accacggcgg tggagatcgt ggcgatcagt tccagcgcca gcgccgaaat
     9061 gaatgtcacc cgcagcaccg ccagcgtggc caggcgataa cgttcgctgg catcgcgtac
     9121 ttcgtttccc cgctccttgc tgcgtccgag catttttaat gttgcgatcc cctgcaatgt
     9181 atccagaaaa taattcccca ggtatgtgag cgctttccat tgtcgctttg ttagcgcttc
     9241 tgatgcccag ccgatcagga ccatgaatac tggaatcaaa ggcagggtga aaacgaagat
     9301 aatccccgtt aaaagatcca ttgggaacac caccgccagg atggtcagcg gcaaggccac
     9361 ggagattaat acttgcggga gatattgcga aaaataggcg tctaacgcgt caaccccctg
     9421 aagcgcggtg gtggtcagtt ctcctgtttt ttcattttta agaaactcag gccccagccg
     9481 attgacctta tccaggagtt cagcccgtaa tctttgcttg atcgcgatcg tcatcctgcc
     9541 ggccagcgcc tctgaaatga tggtcaagac cacacgagag aagatcagca agagaatgaa
     9601 cagcaggatc gaactgactt ccttaagcgt gcgttgatcg ataagcacgc cggaaatgac
     9661 gcggctcagc tggtaggatt ggattaccgc gatccctccc gccaggaaag aagcaagggc
     9721 tgtcaggaga aataacagcc cgtttttcct cgcttccttg aaaagacggg gatggattct
     9781 catgcggatg cgttagctca tcttaaactc gagtaacccc agcacgtcct gcagcgcggc
     9841 gttgaaaacc tcattcatga tttcatcact tgcctggaat gcccccccat aaaccccgtc
     9901 gaccagggca gcgcgaactt tctttgaagg caggatttga ggcggtttga aggcttcttt
     9961 gatgccatcc ggcataccgc ttctccttaa aaatggaaaa gcttccatcc aggacgcatg
    10021 atacatcccc aatccgtgtg cttctgccac ggccgccact gaggaggatt tccaccaatc
    10081 gtaccagctc acctgcaggt caggtaattc attcacaagc tctcccagcg ccagtttggc
    10141 cgggtcattc ccgccatgac cgttcacaaa caggatgcgt ttgaacccct gatgatgcac
    10201 agacctgacc atatcttcca ctgcgttgat cagtgtctgc actctgaagc tgatggtgcc
    10261 gggaaagtcg acgaaatacg aggaacagcc aaaattcaac ggcggtgcca ccaacacgcc
    10321 tgttctttct gaggcatggc tggccagggt cagcgggatc ttgatgtcag tttccaggca
    10381 aatatagcca tgctgttcgg tcgcgccggt caccagcatg atccggtcat ctctcttaag
    10441 gtattgctcc acttccatcc aggtcatttc accaagcagc atattcacct cgctttttct
    10501 tcttgatagt caagttcatt ataatggaac acgcgctgga agttaaaaaa aaactggctg
    10561 cgctaaaagc cggcagccag catacttctt ctcttcgtgc ggctcacacc acgatattga
    10621 tcagccgccc tttgacataa atgatctgac gggcttcttt accctccatt tgcttctgaa
    10681 taatgtcgct tttaagcgcg cgggttttag cttcctcttc gctgatatct accggtacgg
    10741 tgatacggtc ccgcacctta ccgttcaact gcaccaccag ggtgatttct tcgtccgcgg
    10801 cagccttctc atccacgctt ggccagcggc tggtatgaac ggaatatggc ttgtggattc
    10861 gctgccacag ttcttctgca atgtgcggca ccaccggcgc catcatctga agatagatct
    10921 caatggcttc tttccatgca tcgctaccat aaacgctgtc cttaagatgc gccatctcgt
    10981 tcaaaagttc catcaggctg gagatgatgg tattgaattc aaactgctca aagtccctgg
    11041 aaaccgagcg cagggtctgg tgtacttttc ggcgcagtgt ttttaccgac tcctcgttga
    11101 ccgcgccagt ctgcccgggt tccagaacaa ggctccacgc gcgcctcagc cagcgcatca
    11161 cgccatcaat ccccccgctg ctccacggtc ctcccatgtc ccagcgggcg aagaacatca
    11221 ggtaagcgcg cgttgcatcc gccccatagg tgtgaaccag gtcatccgga gagaccacgt
    11281 ttccgcggct ctttgacatc ttttctgaat cttctcccag caccatcccc tggttgcgca
    11341 gttgcagcat gggctcgttg ccttttgtga ggcccatatc gcgcaacgct ttatggaaaa
    11401 aacgggtgta gataaggtgc atgttggcat gctcaatgcc gccggtgtaa atatccactg
    11461 gcatccaata atcgtattcc tgcgggtcga aaggaccttt atcgtaatcc gggctgaggt
    11521 agcgcagatg ataccaggat gagcacacga atgtatccat cgtgtcggtt tcgcgttctg
    11581 cgggcttacc gcattgcggg caggtggtga atcgccaggt cgggtgcagt tttaaggggc
    11641 tttccccggt tggcttccac tccacatcat caggtaatnn nnnnnnnnnn nnnnnnnnnn
    11701 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    11761 nnnnnnnnnn nnnnnnnnac cgccatgatg gcgcccgtgc cataggtcat cagcacatag
    11821 tcagagatcc aaaccgggat cttttcccca ttaacggggt taatggcgta tccaccggta
    11881 aaaatgccgc tcttttcttt ttctgtcgat tcgcgttgaa tatctgattg gcgagaagca
    11941 tccaaaacgt attgatccac ttctgcccgt tgttcaatgg tggtcagctt ctccaccagt
    12001 ggatgttcag gcgccaatac catgaacgtg accccccaca gggtatccgg gcgggtggta
    12061 aaaacctcca gcgcatctcc tgcttcggtt ttaaaggtca ccagcgctcc ttctgacctg
    12121 ccaatccagt tcgtctgcag cgtgcgcact ctctcaggcc aatcaagccc atcgtaattg
    12181 accagttcat cggcgtaacg ggttgtcttg aaaaaccatt gtttaaggtc ttttttgatt
    12241 accggtgtac cgcaacgctc gcagtggcgg tcatctcccc acacctgttc ccgcgccagg
    12301 gtggtattac agtgggggca ccagtccacc ggagatagtt tctggtaagc gagattatgt
    12361 ttgaccagct ggatgaaaaa ccactgcgtc cagcggtagt atttctcatc agaagacacg
    12421 atctccctgc gccaatcgat catcgttccc atactgcgga attgttcacg cattttttgg
    12481 atgttggcgt aagtccactt acgcgggtgt atgttgtgtt tgatggcagc gttctcagcc
    12541 ggcaagccga acgcatcaaa acccatcggg aacataacat taaagccgtt catgcgtttg
    12601 taacgcgcgc gcgcgtcaga gggcgaaatt gcataccaat gaccaatgtg aaggtctcct
    12661 gaaggataag gcagcatggt aagagcgtaa aacttggggc gcttcttatc aatatccgca
    12721 tgataaagac cggctgcctc ccattggcgc tgccacctgg gctcaatttc tgcggggata
    12781 tattctggaa cttggcgggc cattcttctc tccaatcaaa gcattaaaat aaaaaacctt
    12841 cttccctaca gggacgaagg aatcctccgc ggtaccaccc tgcttgcctg cagttgacgc
    12901 agaccgctta ttagcgctaa cgggcttacc cgttaccggt tactgcactt caccggaaaa
    12961 gctatcctct cggatttcag gcgagttcgg tccatgtgtt ccgcaacaca caccgggctc
    13021 ccacctcgct ctcccggctc gctgtcggat gacctactac tcctgcgttc gctctagtgg
    13081 acccaggggg attcgaaccc ccggccttct caatgccatt gagacgcgct cccaactgcg
    13141 ctatgggccc ggatattacc cgtttgttgc tcgagcctgc cgtgctagtg gacctggggg
    13201 gattcgaacc cccggcctct tcagtgcgat tgaagcgcgc tcccaactgc gctacaggcc
    13261 cgttcacaaa ggtagccaga ttttaatcga gacatggcgc agtgtcaaga aagaaattgg
    13321 ctaaagaaat ttaggatgta tgcttttctc caatcctgct tcaggctata atcctcttat
    13381 tcaggagttc atcgatgaca gaaaaaagat tcgcccactt acacgaatta atgaagcaac
    13441 accagcttaa aaccatcatc ttgaacccgg gacccacgct tgcttacctc accgggttgg
    13501 attttcacct gatggaacga ccaaccatgn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13621 nnnnnnnnnc agcgaaatgc gcagagcagt gcaaatcgcc caacgttctc tgctggatat
    13681 tctcccgctg gtcaaaccag gtgtgaacga aaaaacgatt gcagctgaac tgaccattca
    13741 actgctgcgg aatggttctg attctgagct gccgtttcag cccatcgtag cgggggggcc
    13801 gaacagcgcc aacccgcacc tggtgcccac agagcgccct ctccaatcag gagatttgct
    13861 ggtcatcgat tggggcgcgc gctcgaacgg ttattgcgca gacctgacgc gcactttcgc
    13921 aatcggtaat atagaccccg agttaaaggc catctacgaa gcggtgaagc aggctaactc
    13981 aaccgggcgt gctgccagta aacctggagc gactgctgga gaggtggatt tggccgcccg
    14041 agcagtgatt gaaaacgcgg gatacggcgc ctttttcact caccgtactg gtcatgggct
    14101 ggggttggaa gaccatgaac cgccttacat cttcaacggg aattcactag cattacgccc
    14161 ggggatgacc tacacggttg agccaggcat ctacttgccc gggcgagggg gtgtgcgtat
    14221 tgaagacaac ctggtaatta cgcataccgg ctgcgaaaca ttcagcgacc tttcacgcga
    14281 actgatgatt ctctaagaga acaactggag ctccaaatgg ctaaaacctt aaccatggcg
    14341 actgccgaac cctttttcat ccccgcagga aaaacaggat gcctgctcat tcatggtttt
    14401 accggcacgc caaaagaaat gcgcatgctg gcagatgcgc tggctcttga aggttatact
    14461 gttcttgctc cgcgactgtt tggtcatgct acaaatccgc gggacatgag ccgtgcgcgt
    14521 gctgacgatt ggatcgccag cgttgaagat ggtttgaacc tgttaaaagg gtgtacagac
    14581 acgcaggtcg taatgggctt atctatgggc ggtgtgcttg ccctcatcgc cgcggctcgc
    14641 tttgattgcc gcgcagcggt taccttttcc gctccctgct cactccctca tgatccgcgt
    14701 gcctggttgt taccgcttat cggcaggttg aatctaccca tcaagaaagg gaaacctgac
    14761 tggcgcgacc tcgcggcagc acaggagcat aaggattacc cctattaccc aagccgggcg
    14821 atcatcgagc tggacagatt gatcaagctc atgcagggtg aacttgagaa catccgcgta
    14881 cctgttatgc tggtgcaatc aagagcagac cgggggattc cgccagaaag ccttgaaacg
    14941 atttacaatc gcctgccaac agccgaaaaa accagcttct gggttgaaga cagcggacat
    15001 gtgatcattt gtgagccaga aagagaaagg atcttctctc aagttggagc attcttgaag
    15061 caggtcttaa aagacgcatg aatcgcaacc tgctgctggt gacaatctcc ctgttcgcct
    15121 ggggaatggg tgaagggctg tttatttact ttcaaccgtt gtacctgcag gaatttggcg
    15181 cggacccggt aatgattggc agtatttttg gcgtgatggg tgtcgcgatg gcggtcttcc
    15241 aaataccctc agggtatctt tcagatcgtt tcggttccag gtcgatcatg tgggcttcgt
    15301 ggtttttagg aacctctgct gcctggacaa tggcgctggc caacaccctg cccacgttcg
    15361 tggtcggcct gattctttac ggtctgacca gttttgtggt cgcccctatg aacagctata
    15421 tcactaaagt gcgcggcgat tggagcgtgg gacgggcgct taccttccct tcaggttttt
    15481 accaccttgg ggcggtgctg ggtcccatca gcgggggatt gatcgccgac cgcctggggt
    15541 tgaagaccgt ttatatggta gccgccatta tttttataat ctccaccatt atcgtccttt
    15601 tcacctctca agcgccagaa acccatcacg cggaccaggt tgatgccacc acaaagggga
    15661 cattgaaaaa tccgcgtttc tttgctttcc tggtgatcac tctgctgagt atcttcgcca
    15721 tctatttgcc gcaacccttt acccccagtt tcctgcaaaa tcaacaggcg ttttcgcgct
    15781 ccatgatcgg gatccttggc gctgtcggca gcctggggaa cgccgttgcc acccttgccc
    15841 tgggaagttt gccctcggtc atcggcttta tcatcggtca ggtctgggtg ctggtctttt
    15901 cacttacatt tcttttcgcc cgcgtgcctt tctggttcgg cttcgggtat tttttcatcg
    15961 gcggttacag gctgtgccgg tccatggtgc tcgccatctc ccgaaccctg gttaacccac
    16021 gccagaccgg cctggcttac ggtatggtag agaccatgaa cgccatcgca gttataattg
    16081 cgcctgtaat agccggggtg ctttacaaga ataacccatt gtcgattttc tctgtttccc
    16141 tggttattat ctcaatcacg attatcttga acctgaccgt ccttgtgaga caaaaaagag
    16201 gtcgcttgca tgagcctgaa aattgatacc tttacgtcca gtatgatgag taacaatgtg
    16261 gtcatcctgc atgatgatga aacccgccat gctatcatta tggatccttc ttttgagcca
    16321 gagcaagtgc tcacatttat caacaataac tcgctgtcag tggaaatgat actttgtacc
    16381 cacgggcatt ttgatcactt cgctggtctc gcttacctgc tcaccaagct gcaccccaaa
    16441 ccaaaggtcg gtctgcacga gagcgatctg gctttgtggc gagccggagg aggggcggtt
    16501 cattttcgca taccaatagc cgtccccgaa gaccctgaca tcttcctcac agagaatcaa
    16561 accatccatt tgggtgacgg gcaaattcaa atcaggcaca cgcctggcca ctcacctggt
    16621 tctgttgttt tttacatgga aagtctccat actgcggtag t
//
LOCUS       DAOTLW010000039        16601 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_3881_length_16601_cov_9.842802, whole genome shotgun sequence.
ACCESSION   DAOTLW010000039 DAOTLW010000000
VERSION     DAOTLW010000039.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 16601)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 16601)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16601
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_3881_length_16601_cov_9.842802"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            83..346
                     /gene="rpsT"
                     /locus_tag="P5516_06865"
     CDS             83..346
                     /gene="rpsT"
                     /locus_tag="P5516_06865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HRT92190.1"
                     /translation="MANIKSAIKRNKQNEKRRLRNRVYRGSARTFVARARVALARQDL
                     ETAESATKAAVSALDKAAQKGVIHKNNAARRKSRLMKRLAALK"
     gene            463..2226
                     /gene="argS"
                     /locus_tag="P5516_06870"
     CDS             463..2226
                     /gene="argS"
                     /locus_tag="P5516_06870"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HRT92191.1"
                     /translation="MFELEQKQVEEKIHAYCRQQGLPVIEIKWTWIPFNGQWGISTSF
                     FQLAAASFGEKKGNVAARASEIAENLAVSLAKLPAFEKIEAVKGYLNLYFSTSAYFQQ
                     VLNSVLEEQDKFGHGERLHQRVMVEFSHPNTHKAFHVGHLRSAFLGDVLARILDAAGY
                     EVIRANYPGDIGLHVIKWLWNYLKFHKGEKPDADITRWMGSIYAEAVRRLEQDPALED
                     EIREMYNRWDRRDPEVVACWKETRQWSLDGFNAMYNLLGIHFDRYYFNSMFEVPGKEM
                     VEELLQKGIADDERPENGAVVVKIDEKLGLKEEKYRVMVVLRSDGTALYATEDLALAR
                     QKFLDYPDLQKSFYVVDVRQSLHFQQVFKTLELAGYEWATRCQHLPYELVNLPGNVVM
                     ASREGTVVLLEDLIREATDRALTVVQEKNRELSAEKQLEIARAVGIGAIRFPMLARES
                     NKVVTFDWESALDFNGQAAPYIQYAYVRANSIMRKIGDRIPPDLAPTYELTQPEITLV
                     DLISRMPDEIQKAARDLRPLIITNQAYELARAFNDFYTQCPVLSAEEPARSVRLRLVA
                     ASRQAIANSLAILGITAPQAM"
     gene            2259..>3193
                     /locus_tag="P5516_06875"
     CDS             2259..>3193
                     /locus_tag="P5516_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HRT92192.1"
                     /translation="MTPIKTLIMGAAGRDFHNFNVFYRDNKDYEVVAFTATQIPNIEG
                     RKYPAELAGSLYPAGIPIYPESELVDLIKKFKVDQVVFAYSDVPHEVVMHKASTVLAA
                     GADFRLMGVNSTQVKSVKPVVSVCAVRTGSGKSQTTRRVSTILRELGYKVAAIRHPMP
                     YGDLVKQKVQRFATYADLDKHECTIEEREEYEPHIDNGVIVYAGVDYEAILREAEKEV
                     DIILWDGGNNDFSFYQSDLSIVVADPHRAGHEYKYHPGETNVRLADVFVINKVDTAEP
                     GDVIAVRNTLHELNPGAIIIEGASPLFVDDPDAIRG"
     assembly_gap    3194..3293
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            3466..4725
                     /gene="obgE"
                     /locus_tag="P5516_06880"
     CDS             3466..4725
                     /gene="obgE"
                     /locus_tag="P5516_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HRT92193.1"
                     /translation="MFIDEAKILCRSGRGGAGVVHFRREKYVPRGGPDGGDGGRGGDV
                     IFRVDEKLNTLLRFRYQRKFVAEDGGNGRGSNMSGKGAPNLVIPVPPGTIIYDHATNA
                     LLGDLVEPGQELTVCKGGRGGRGNQHFATSRNQAPRTAEKGEPPEEREIRLELKLLAD
                     VGIVGVPNAGKSSFLAAVTNAKPKIADYPFTTLEPNLGVASLDSETDLVLADIPGLIE
                     GAHQGVGLGDAFLKHIQRTKVLIHILDGSSTDPLADFSQINQELALFDAELAEKPQVV
                     VVNKADLPGVKESAVKVIKQLEKMGYPTFLISALTRENLTPALWKAHEVLLKVPQKEI
                     KPELPVYKPGGDPRDFSITRENTGWRVSGAAIERAAEMTYWEHFGSVRRFQRLMVALG
                     VDRALREQGIKNGDTVYILDYELEWQD"
     gene            4727..5335
                     /gene="nadD"
                     /locus_tag="P5516_06885"
     CDS             4727..5335
                     /gene="nadD"
                     /locus_tag="P5516_06885"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HRT92194.1"
                     /translation="MTAYQRIGIFGGTFDPPHVGHLILAEECRTQLSLDLLLWVVTDN
                     PPHKRYTNVSPIQDRFMLVEKAIQGNPSFRTSRVDIDRPGPHFAIDTVNILKEEYPGA
                     EMYYLMGGDSLHDLPTWNKPQQFIDLCDGIGVMRRHADQVDLSALEKVLHGISEKVKI
                     VEAPILEISSKQIRQRIAEGMGYRYYLRDAVYQAVCELGLYR"
     gene            complement(5401..6252)
                     /locus_tag="P5516_06890"
     CDS             complement(5401..6252)
                     /locus_tag="P5516_06890"
                     /inference="COORDINATES: protein motif:HMM:NF028425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HRT92195.1"
                     /translation="MKTTKLLILLALALSLMACTFSVNVPEVKTGQTRTFEVSEASPP
                     ADEVSSIKIEMGAGSLNLSGGADKLVEGTISYNIDSWKPGVTRQGNAVTIKQSTSTSV
                     AIPADTYKNSWDLKLGSTPIELTLATGAYEGDLELGGLAITALSVTDGASKSIIRFNE
                     PNAANLEQFSYKTGASDVRLEGLGNARASHIEFNGGVGRYTLDFSGINDNDIAVKMDC
                     GMSDITLVIPENVRSIITINGEMNNTNFTGTWTVKDNVYQSGTTGVLIEIEIDMSVGN
                     LNLIRHE"
     gene            complement(6290..7318)
                     /locus_tag="P5516_06895"
     CDS             complement(6290..7318)
                     /locus_tag="P5516_06895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92196.1"
                     /translation="MKKTCAFATVILLFLLAFVVSPVHAQEPQPGNTISGDQVIIGNN
                     FTLEKNETLEGSLLIIGGTASTAADSKINGDLVLIGGTLTIDGAVEGDIVSIGGVVNL
                     QDSAVVNGSLTMIGATLSRSPTAQISGSISEETPRFLNFDFLRDNGLKPPLHLKTNPL
                     NRILTATFQALVMAILAVLVGLLLPRNLKNTAIALVREPVVTGGVGLLAMVVAPIILI
                     LLSITIILIPVTLLAAIAFALAVVFGNIVIGYEIGNRLSAVFKDTWHPSIAAGIGTLL
                     LSLVTGFAGVIPCVGWVLGFLASIIGLGAVVISRFGSTKYATKVISAVIQPAEEDLPT
                     EPPAAPEE"
     gene            complement(7315..7857)
                     /locus_tag="P5516_06900"
     CDS             complement(7315..7857)
                     /locus_tag="P5516_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HRT92197.1"
                     /translation="MMKHYPYEGWILDDIDLDETRRADLEAHLALCPQCAQLSRSLRI
                     ALQAIRNAPEQTAPAGFVHKFVSSFEARRRLQERKQARTLAIVFASTALIVVLVSLYV
                     FLPELSLISLAAGAISAVVGLLEGFQQVVSFISNAYKNLSPFTLIISGLVFAGWVLLA
                     SLTLGLSVWKLAFRKSEVKQ"
     gene            complement(7835..8428)
                     /locus_tag="P5516_06905"
     CDS             complement(7835..8428)
                     /locus_tag="P5516_06905"
                     /inference="COORDINATES: protein motif:HMM:NF019265.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRT92198.1"
                     /translation="MKQIDPIWIERARHGDEEAFTMLVEAYQTPVFNLCYRMLGESTA
                     AEDAAQETFWRVWQKIKSYDPQRSFSTWVLSIAAHYCIDQQRKQKITIFELDEYPDFD
                     LGDPHMPNPEITVITNEEEETLHRMINQLNPPDRAAVILKYWYGCSEEEISQMLSLTV
                     SAVKSRLHRARRQLAEVVTKNQSTTLQRGSYDEALSL"
     gene            complement(8530..9165)
                     /locus_tag="P5516_06910"
     CDS             complement(8530..9165)
                     /locus_tag="P5516_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HRT92199.1"
                     /translation="MVKRLILVAGNIGSGKTSLTERIGERLGWHTAYESVVDNPYLPD
                     FYANMREWAFHLQVYFLGHRAQQHLDMYHDPRSAIIDRSIYEDAFIFARALYSMGNIN
                     ERDYGTYRKVFDLITATLPHPSLLIYLKCPVDTLIKRIQSRGRDIESSISRDYLALLE
                     SYYDDWTSNFDLCPILTIRTDDLDFVHEPRHLNTVVKKIKEKLAGREEIVF"
     gene            complement(9359..10216)
                     /locus_tag="P5516_06915"
     CDS             complement(9359..10216)
                     /locus_tag="P5516_06915"
                     /inference="COORDINATES: protein motif:HMM:NF014679.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRT92200.1"
                     /translation="MHKVKVITDTDSSLPADVAARYRIDQVPITIQFGDDTYEAGVNI
                     NDKELFEKIDALGSLPTTAAPAPAACRSAFAKAFAEGADSIICITVGSKISRTYDSAV
                     TTAKDDFPDRLITVVDSTCLSLQQGFMALSAAEALEAGYTHQQAVDAALQLLPRSHLY
                     GSLTTLKYLAMGGRIGQFPAKMANLLDIRPIMTMIDGKLGLLEKVRTHRAAMNRLIEL
                     FEKSTSGKPIVKAGIVHVNNLTDAALLESRLRESLPMPDEILVAEFTPGLSVHGGTGL
                     IAALLVTED"
     gene            complement(10530..13124)
                     /locus_tag="P5516_06920"
     CDS             complement(10530..13124)
                     /locus_tag="P5516_06920"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004748 - ribonucleoside-diphosphate
                     reductase activity, thioredoxin disulfide as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobalamin-dependent
                     ribonucleoside-diphosphate reductase"
                     /protein_id="HRT92201.1"
                     /translation="MDTPPRTAQKSLLDLGGIPNNLPTVTLSDNARQVLLKRYVRRNP
                     DGSPAETVEEMFWRVASTIANIETRWSADVDALAQRYYILLSSKAFFPNSPTFTGAGT
                     PLGQLAACFVLPINDDMGRDSAGIFQTLRDAALIQQTGGGNGFSFSRLRPKGSHVKTS
                     AGQATGPVGFLRVYDHAFGEVAQGGTRRGANMAVLRVDHPDIEEFIACKTNENQITNF
                     NISVGITDAFMRAVEDDVEWNLRFPDLSDPRAKKFIGTFEQAEDAGIPFRIYKTIRAR
                     DLFQRIVKQAHHNGEPGLLFLDAANRANPVPHLYQLEATNPCGEQWLGPYENCCLGSI
                     NLAQHLGPDHSVDWIKLRENTVLATRFLDDVVDANAYVPAVPQLKEAAMRNRRIGLGI
                     MGLADLMYHVGVRYGSPAAEDFASQVMEFVRYHAMKTSIELARERGPFPSIKGSIYDP
                     ENLVWQPPVSLIETQQYWGRPTIDWSEISEGIRRYGIRNAAQTTVAPTGTIATVAGCE
                     GYGCEPVFALAYIRHVNDNGKDLKLTYTSPAFEQALLEAGVSEMDRQDIYDQVMKDGS
                     CQHIKSVPEMVKNTFVVSADISAEEHVRMQAALQAFVDNSLSKTINFPENATEEDVAT
                     AYLRAWELNCKGITVYVTGSREEVVLETNATASKKAPAADDAMPDQIKLWQESKKPRP
                     RSLIGRTFSIETPLGKAFITINENDAAQPFEVFINTAKAGSDTAAVSEAIGRLISYVL
                     RLASPIEPSQRLTDLTRQLLGIGGGRSLGFGPNRVRSLPDGVAQVLEEYLDERAIRLG
                     KADGKPGVVQQYLINDITDLTPPVEEPPASPPVVQRIGDLCPECGVAAVINEEGCRKC
                     YACGYSEC"
     gene            complement(13133..13582)
                     /gene="nrdR"
                     /locus_tag="P5516_06925"
     CDS             complement(13133..13582)
                     /gene="nrdR"
                     /locus_tag="P5516_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HRT92202.1"
                     /translation="MLCPYCQKDQSRVIDTTHDSRGGIRRRRECEHCQQRFSTYERPI
                     LATPLIIKRSGTREQFNREKLLEGIRISCAKRPVSADDINHLVNEIETALQKMGREEV
                     SSRVVGDMVIDGLKRLDQIAYIRYAIVYLQLTDLQSIRKEIDRLLTS"
     gene            complement(14142..15338)
                     /gene="ftsZ"
                     /locus_tag="P5516_06930"
     CDS             complement(14142..15338)
                     /gene="ftsZ"
                     /locus_tag="P5516_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRT92203.1"
                     /translation="METNRTPQYASPRSSFEAESKSPIETFARIKVIGVGGGGCNAVN
                     RMIEQGAQGIEFITVNTDAQALLMSKAPTKIRIGDKTTNGLGAGGNPEVGRKSAEESY
                     EELTQVLKGSDMVFITAGMGGGTGTGAAPIIAKIAKDSGALTIGVVTRPFTFEGAKRL
                     QSAEQGIARLKEQAHTLIVIPNDRLLQMVDRKSTLQESFRLADEVLQQGIQGISEVIT
                     IPGLINLDFADVKNVMSEGGAALMAVGRASGEDRAIKAAEMAIFNRLLDITIDGARGI
                     LFNVTGGPNLTLHEVNQAAAIIKETAHPEVNLIFGAVINPDMGDDVRITVIATGFDRA
                     NGSPIVRKPVTKVNDESRKPAFQTNTEIKEVTPVNTPVNEFVPVKMDTRNLDLPTFLR
                     IHLDEK"
     gene            complement(15392..>16601)
                     /gene="ftsA"
                     /locus_tag="P5516_06935"
     CDS             complement(15392..>16601)
                     /gene="ftsA"
                     /locus_tag="P5516_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRT92204.1"
                     /translation="TKICTLVARLEGDTDMRILGVGIEPALGIKKGNVVDISTATQSI
                     SRSIEKAQRTSGLEIESALVSLAGSHVMAMNSRGVVGISGGVISQEDVDRALEAAMAV
                     AMPHNREIIHVIQRGFTVDGQEGIQSPVNFSGFRLEVEAHIITAAAATVENLRQCVNG
                     CNVDVLQFVLNPLASGEVVLNKPERDMGVCICDIGGGTSDLAIYINGDVWHTDVLPVA
                     GNQITNDIAIGLHISPEQAEQIKKQYGHAITGEVGEGEFFTLRTFGDDQPKQISRREL
                     AYIIEARVEEIFQLLHQEVRRSGYENLLPAGMILTGGTSQLPGIRQLAADVLNMPVRV
                     ARPENLVGLVDQLQSPAYSTSVGLLKWSELMTQPSYAVTHPRKPKTSGFKPSVLNWDK
                     LKVLVERLLP"
BASE COUNT         3933 a         4066 c         4724 g         3778 t
ORIGIN      
        1 ggtgatgcgg tagaattctt gcgccacctg aacagtgctt accctcgacc ctcgcactga
       61 cggaattttt tggaggaaca agttggcgaa tattaaatct gcaataaaac gcaacaagca
      121 aaatgaaaaa cgaagactgc gcaaccgggt ttaccggggt tcagcgcgca cgtttgtcgc
      181 ccgcgcccgg gtggcattgg cccgccagga ccttgaaacc gcggaaagcg cgacaaaggc
      241 tgctgtgagc gccctcgaca aggctgcaca gaaaggcgtt attcataaga acaacgcagc
      301 gcggcgcaag agcaggctga tgaagcgcct ggcagcatta aaataagatc gttttttcaa
      361 aaccgagcgg gaggagaaac ctcccgctct tgctgttaaa aaccagagcc gtttttttcc
      421 ctatgatata atccatctgc ctatcgagga aaacgaaaaa acatgtttga attggaacaa
      481 aagcaggtcg aagagaagat ccatgcttac tgccggcagc aggggctgcc agtaatcgaa
      541 ataaaatgga catggatccc tttcaacggc cagtggggaa tcagcacctc tttcttccag
      601 ctggctgccg cttcttttgg tgaaaagaag ggcaacgtgg cggcacgcgc cagcgaaatc
      661 gctgaaaacc tggctgtgtc tctcgcaaaa ttgcccgcgt tcgaaaaaat cgaagcggtt
      721 aaagggtatc ttaatctcta tttttcaaca tctgcttact ttcaacaagt gcttaattcg
      781 gttttggaag aacaggataa gtttggtcat ggagaacgct tacaccagcg ggttatggtt
      841 gagttctctc acccaaacac acacaaagcc ttccacgtgg ggcacctgcg cagcgctttt
      901 ttaggcgatg tactggcgcg tattttggat gctgctggtt atgaagttat cagggcgaat
      961 tatccgggcg atattgggtt gcatgtgatc aaatggttgt ggaattacct gaagttccat
     1021 aaaggggaaa aaccagacgc tgatattaca cgctggatgg gatcgatcta cgcggaggcg
     1081 gtcaggcgcc tggagcagga ccctgcgctt gaagacgaaa taagggagat gtacaaccgc
     1141 tgggacagac gtgaccctga agtggtggcg tgctggaaag agaccaggca gtggtcgctg
     1201 gatggcttca atgccatgta caacctgttg ggcattcact tcgatcgtta ttacttcaat
     1261 tcgatgtttg aagtgccggg caaggagatg gttgaagagt tgctgcaaaa agggatcgcg
     1321 gacgatgagc gcccggaaaa tggcgctgtg gtcgttaaaa tagatgaaaa actcggattg
     1381 aaagaagaaa aataccgggt gatggtcgtt ctgcgatcag atggaacggc gctgtacgcc
     1441 acagaggatc tggcgctggc gagacagaaa ttcttggatt atcctgacct gcagaagtca
     1501 ttttatgtag tggatgtacg gcagtcattg cactttcaac aggtgttcaa gacattggaa
     1561 cttgccgggt atgaatgggc aactcgctgt caacatttac cttatgaact ggtgaattta
     1621 cccgggaatg tggtgatggc ttctcgagaa ggcacggtag ttcttctaga agaccttatc
     1681 cgggaggcaa ctgaccgcgc cttgaccgta gtgcaggaga agaaccgtga gctctctgct
     1741 gaaaaacaac tggagatcgc gcgggcagtg ggaattggtg caatcaggtt cccaatgctt
     1801 gcgcgcgaga gcaataaagt ggttaccttt gactgggagt cagcactgga ctttaacggg
     1861 caggcagccc cttatattca atacgcgtat gtacgcgcaa atagcattat gcgtaagata
     1921 ggagatcgca ttccgcctga cctggctcca acgtatgaac ttactcagcc tgagatcacc
     1981 ctcgttgacc tgatcagccg catgccggat gagatacaaa aagcagcccg ggatttgaga
     2041 cccttgatca ttacaaacca ggcgtatgaa ctggcgcgcg cgtttaacga tttttacacg
     2101 caatgcccgg tgctgagtgc cgaagaaccg gcccgctccg tacgattgcg acttgtagca
     2161 gcttctcgac aggcaattgc aaacagtctg gcaatactgg gcatcacagc cccacaagcg
     2221 atgtgaaaat aatctaacaa aaaaaggaga ccattcaaat gaccccaatt aaaaccctca
     2281 tcatgggtgc tgccggtcgc gattttcata atttcaatgt tttctatcgc gacaacaagg
     2341 attatgaagt cgtcgctttc actgccacgc aaattcccaa tatcgaaggc cgcaaatacc
     2401 cggctgagct ggcgggttct ctctacccgg cggggatccc catctacccg gaaagtgaac
     2461 tggtggacct gattaagaaa ttcaaagtag accaggtggt tttcgcgtac agtgacgtgc
     2521 cgcatgaagt ggtgatgcac aaagcgtcta ctgttttggc ggctggggcg gacttccgat
     2581 taatgggggt aaacagcacc caggtcaaat cggtaaaacc tgtggtatct gtttgcgcgg
     2641 tgcgtaccgg gtcgggtaaa agccaaacca cacgccgtgt ttctacaatt ctgcgcgaac
     2701 tgggttacaa ggtggctgcc atccgccacc caatgcctta tggcgacctg gtcaagcaaa
     2761 aggtgcagcg atttgccacc tacgctgacc tggataagca tgaatgcacc attgaagagc
     2821 gcgaggaata cgaacctcat attgacaatg gcgtcatagt ctacgctggc gtggattacg
     2881 aagcgattct gcgcgaagcg gaaaaagaag tggatattat tttgtgggac ggtggaaaca
     2941 acgacttctc attctatcag tctgatttga gcattgtcgt ggctgacccg caccgagccg
     3001 gccatgaata taaatatcac ccgggcgaaa cgaatgttcg gctggcagac gtgtttgtga
     3061 tcaacaaagt cgataccgct gaacccggcg atgttatcgc cgtgcgcaac acactgcatg
     3121 aattaaaccc aggcgcgatc atcatcgaag gcgcttcacc gttatttgtg gatgaccccg
     3181 atgccatccg tggnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnctcaatc
     3301 gcattatcaa ggtgaataaa ccgatgcaac gcgtgcgtta cgaactgcag gaaattggta
     3361 aaccagacct ggaagacatt ttaaaaggga aattctccaa gaagtaacag aaaagcaaaa
     3421 caggctcggc gcgaaaaagc cgagcctgtt gcgggatgaa acaggatgtt catcgacgaa
     3481 gccaaaatac tctgcaggtc aggccgcggc ggggcgggag tggtgcattt ccgccgcgaa
     3541 aagtacgttc ctcgtggagg gcctgatggt ggagacggcg gcaggggcgg agatgtaatc
     3601 tttcgcgtag atgaaaagct gaacacattg ctcaggttcc gctatcaacg caagtttgtg
     3661 gcagaagatg gcggaaacgg tcgcggcagc aacatgagcg gtaaaggggc accaaacctg
     3721 gtgattcctg ttcccccggg gacgataatt tatgaccacg cgacaaacgc gcttctcggt
     3781 gacctggttg agccagggca ggaactgacg gtgtgcaagg gcggaagagg cggcagggga
     3841 aaccagcatt ttgccacctc gcgcaatcag gcgccacgca ccgctgaaaa aggagaacct
     3901 cctgaagaac gggaaatcag gctggagctg aaactgctcg ctgacgtggg aatagtgggc
     3961 gtcccgaacg ctggtaaatc cagtttttta gcagcggtta ccaatgccaa acccaaaatt
     4021 gcggattatc cttttaccac ccttgagccc aacctgggcg tagccagcct ggacagcgaa
     4081 actgacctcg tcctggcgga tatccctggg ctcatcgaag gtgcgcacca gggagtaggg
     4141 ctgggagacg ccttcctcaa gcacattcaa cgtaccaagg ttctcattca tatactggat
     4201 ggcagctcca ctgacccact ggcagatttc agccagatca accaggaact ggcgctgttt
     4261 gatgctgaat tagcagagaa accgcaggta gtggtggtga acaaagccga cctgccgggg
     4321 gtgaaagaaa gtgcggttaa agtaatcaaa caactggaga agatgggata tcccactttt
     4381 ctcatctcag cgctgacgcg agagaactta acccctgcac tctggaaagc gcacgaagtc
     4441 cttttgaaag tacctcaaaa agagatcaag cctgaactgc cagtgtataa accaggtgga
     4501 gacccgcgtg atttttccat cacacgcgaa aatactggtt ggcgggtgag cggtgcggcg
     4561 atcgaacgcg cggcggaaat gacctactgg gagcatttcg gttctgtgcg gcgcttccaa
     4621 cgcctgatgg ttgcgttagg tgtcgataga gcgctgcgcg aacagggaat taagaacggc
     4681 gataccgtgt atatcctcga ttacgaactg gaatggcagg attgatatga cagcctatca
     4741 acgtattgga atttttggcg gaacgttcga ccctccacat gtagggcacc tgatcctggc
     4801 ggaggaatgc cgcacgcagc tttctttaga cctgctcttg tgggtggtaa cagacaaccc
     4861 ccctcataaa cgttatacca atgtcagccc cattcaagat cgttttatgt tggtagaaaa
     4921 agccattcaa gggaacccat ccttcagaac ttcgcgggta gatattgacc gcccgggccc
     4981 ccattttgcc attgatacgg tcaacatcct taaagaagag tacccaggag cggaaatgta
     5041 ttatctgatg ggtggtgact cgcttcacga tttgccaacc tggaataaac cgcagcaatt
     5101 tatcgattta tgtgatggta taggtgtgat gcgccgccac gccgaccagg tggacctatc
     5161 tgcactcgag aaggtattgc acgggatatc agagaaggtg aaaatcgttg aagcaccgat
     5221 tctcgagatt tcttctaaac agattcgaca gcgaatcgct gaaggcatgg gctaccgcta
     5281 ttacctgcgt gacgcggtgt accaggcggt atgcgagctg ggtttgtacc gttaggcaaa
     5341 aatgatttga tgataaaaaa attccggcgt caacccgccg gaattttaag gttcatccgc
     5401 ctactcgtgg cggatcaggt taaggttccc gactgacata tcgatctcta tttcaatgag
     5461 cactcctgtc gtacctgatt ggtagacatt gtccttaacc gtccaggtgc cagtgaaatt
     5521 ggtgttattc atttcgccat tgatggtgat gatcgagcgc acattctcag gtattacgag
     5581 cgtgatatcg ctcataccgc agtccatttt cacggcaata tcgttgtcgt ttatccccga
     5641 gaaatccagg gtgtatctcc ccacccctcc attgaactca atatggctgg cgcgggcgtt
     5701 acccaaaccc tccaggcgaa catcagacgc cccggtttta taggagaact gctcgaggtt
     5761 tgcggcgttg ggctcattaa acctgatgat ggacttgctg gcgccgtcag ttaccgaaag
     5821 agcagtgatg gcaaggccac caagctccag atcgccttcg taggcgccag tggcgagagt
     5881 gagctcaata ggcgtggaac caagtttgag atcccaggag ttcttgtatg tatcagcggg
     5941 gattgcaaca ctggtagagg tagactgttt gatcgtaaca gcattaccct ggcgggtgac
     6001 gcccggcttc caggagtcaa tattgtaaga aatggtgcct tcgactaact tatcggcacc
     6061 cccactgagg tttaacgacc ctgctcccat ttcgatttta atactggaca cctcatcagc
     6121 aggtggggag gcttcactca cttcaaatgt tcttgtctgt ccggttttca cctcaggtac
     6181 gtttaccgaa aaggtgcagg ccatcaaaga gagggcgagc gcgagtaaga tgagcaattt
     6241 ggttgttttc atttttcaat caccttcatt tcgatagaat aaaacgtttt cattcctcgg
     6301 gggcagcagg cggttctgtg gggagatctt cttccgctgg ctggatcacg gctgagatta
     6361 cttttgtggc gtatttggtc gaaccgaaac ggctgatgac caccgcgccg agacctatga
     6421 tggaagcgag gaaccccagc acccagccca cgcagggaat taccccggcg aacccagtga
     6481 ccaggctgag cagcagcgtg ccaataccgg ctgcaataga aggatgccag gtgtctttaa
     6541 acaccgcaga aaggcggttg ccaatctcgt atccgatgac gatattgcca aagaccactg
     6601 ccagtgcgaa ggcaatggct gccaataacg tcacaggaat aaggataata gtgatgctga
     6661 gcaatattaa aatgatagga gccaccacca tagcgagcag gccaacacct ccagttacaa
     6721 ccggctcccg taccaacgcg atggctgtgt tcttgagatt gcgcggcagc agcaggccta
     6781 ccagtaccgc cagtattgcc atcaccaacg cctgaaaggt agcggtgagt atcctgttca
     6841 atgggtttgt tttaagatgg agcggtggtt tcaaaccgtt atccctcagg aaatcaaaat
     6901 ttaaaaatcg cggtgtttct tctgagatac tgccgcttat ttgcgcggtt ggggaacgag
     6961 agagggtagc gccgatcatt gtgaggctgc cattgaccac cgcgctgtct tgcaggttta
     7021 ccacaccgcc tattgaaaca atatcccctt ctactgcgcc atcgatggtc aacgtcccac
     7081 cgataagaac gaggtcaccg ttgattttgg agtcagcggc ggtggaagcc gtgccgccga
     7141 tgatgagcag actgccctcg agcgtttcat tcttctcgag tgtgaagtta ttgccaataa
     7201 tcacctggtc gcctgagata gtgttacccg gttgaggttc ctgggcgtga acaggcgaaa
     7261 cgacgaaggc gagaaggaag agcaggatga ccgttgcgaa ggcgcaggtt tttttcattg
     7321 ttttacctct gattttctaa acgcgagctt ccacacagaa agcccaagcg tgagactggc
     7381 caggagaacc cagccggcga aaaccaggcc gctgataata agcgtgaaag ggctgagatt
     7441 cttgtaagcg tttgaaatga aggaaaccac ctgttgaaaa ccttcaagca ggccaacgac
     7501 tgcggaaatg gctccggcag ccagtgaaat aagcgaaagc tcggggagga acacataaag
     7561 agaaactagc acgactatga gagcggtaga ggcaaaaaca atggcgaggg tgcgcgcctg
     7621 cttgcgctcc tgcaggcggc gccgggcttc gaacgaagaa acgaacttgt gaacgaaccc
     7681 ggcaggggcc gtctgctcag gtgcgttgcg gatggcctgc agcgcgatgc gcagtgagcg
     7741 tgaaagttgg gcgcactgcg ggcaaagagc caggtgcgct tccaggtcgg cgcgccgtgt
     7801 ttcatcaagg tctatgtcat ccagtatcca accttcatag ggataatgct tcatcatatg
     7861 atcctctttg cagcgttgtt gattgatttt tggtcacgac ctcagcgagc tgtcgcctgg
     7921 cgcgatgcag gcggctctta accgcgctga ctgtcaatga aagcatttga ctgatttctt
     7981 cttccgagca tccataccaa tattttaaaa tcaccgcggc gcgatcaggc gggtttagct
     8041 ggttgatcat acggtgcagt gtttcctctt cttcattggt gatgaccgtg atctcggggt
     8101 tgggcatatg cgggtcgcca aggtcaaaat cgggatactc atccagttca aaaatcgtga
     8161 tcttctgctt gcgctgctgg tcaatgcagt aatgcgccgc gatggataac acccaggtgc
     8221 tgaaagagcg ctgcgggtcg tagcttttta ttttctgcca aacgcgccag aacgtttcct
     8281 gggcagcgtc ttcagcagct gtgctctcgc cgagcatgcg atagcacagg ttaaaaacag
     8341 gggtttggta ggcttcaacc aacatggtga acgcttcttc atctccatgg cgggctctct
     8401 cgatccaaat tgggtcaatc tgcttcattg ccatcccgct ttattttatt gtctagcaaa
     8461 tatacgcaga ggggtgaaat aggttgcagc cacattttta ctactttact gctgattgag
     8521 caggaagtat taaaagacga tttcttcccg gccagcaagc ttttctttga tctttttcac
     8581 cacagtattt aggtggcgcg gctcgtgaac gaaatccagg tcgtctgtgc ggatggtgag
     8641 gatcgggcag aggtcgaaat tgctcgtcca gtcatcgtaa taagattcaa gaagcgccag
     8701 gtaatcacgg gaaatggaag attcaatatc gcgcccgcgc gattggatgc gttttatcag
     8761 ggtatccaca gggcatttga ggtatatcaa cagcgaggga tgaggtaagg tggcggtgat
     8821 gaggtcaaag accttgcggt aggtgccgta atcgcgttcg ttgatattcc ccatggaata
     8881 gagcgcgcgg gcgaagatga aagcatcttc atagatgctg cggtcaatga tggcggaacg
     8941 cgggtcatga tacatatcca gatgttgttg agcgcggtgc cctagaaaat agacctgcaa
     9001 gtgaaaagcc cattccctca tattggcgta gaaatccggc aggtatgggt tgtcgacgac
     9061 agattcgtaa gcagtgtgcc agccgaggcg ttcaccgatg cgctcggtga gcgaggtctt
     9121 accggaaccg atgttacccg cgacgaggat gagtctcttg accatgcgcg tgctcctgta
     9181 accaagaata catctattat acggcaatgc gaggaaggca ccgttacagg cagtatttca
     9241 gccggaagga gagggtgaaa aacagccgcg ttgggatttt attcgcctga taaggtgatg
     9301 gatggcatca caaaccgccg ggcttgccgg cggtaatttt ctgtgaaagg ggaaacgctc
     9361 aatcttcggt aacaagaagg gcggcgataa gaccagtacc gccgtgaact gaaagcccgg
     9421 gggtgaattc ggcgacgaga atctcgtcag gcataggcag gctttctcgc agtcgcgatt
     9481 cgaggagggc ggcatccgta aggttgttga cgtgcacgat accggcttta acgatgggtt
     9541 tgccagaggt ggacttttcg aagagttcaa tcaggcggtt catagcggcg cggtgggtgc
     9601 gcactttttc gagcaggcca agcttgccgt cgatcatggt catgatgggg cgaatatcga
     9661 gcaaattggc cattttcgcc ggaaactgac cgatgcgacc gcccattgcc aaatacttta
     9721 atgttgtcag cgagccgtaa aggtgggaac gcggtaacag ctgaagggcg gcatcaaccg
     9781 cctgctggtg cgtgtagccg gcttccaggg cttcagcggc ggagagcgcc atgaaaccct
     9841 gttgtaatga aaggcaggtg gaatcaacca cggtgataag tctatcgggg aagtcgtctt
     9901 tggcagtggt gacggcggaa tcgtaggtgc ggctgatctt gctgcccacg gtgatgcaga
     9961 taatggaatc ggcgccctcc gcaaaggctt tggcgaaggc tgatctacag gcagcgggag
    10021 caggggcggc ggtagtgggc aggctgccaa gcgcgtcaat tttttcaaaa agctctttgt
    10081 cgttgatgtt gacaccggct tcataggtgt catcgccaaa ctgaatggtg atgggcacct
    10141 ggtcaatacg atagcgcgca gcgacgtcgg ccggcaggct ggaatcggta tcggtgatga
    10201 ctttgacttt atgcatgcat actcctaaac cacgaccaaa ttttcttcag cagaatgagc
    10261 attcaccttt atgcattaac catctatttg cgttttcgat tcgctgaaaa ccactcaacc
    10321 agaactgcat ggtcaaccct taaaaacgca ggcggttgga cgatcaaacc cttgctgaat
    10381 aaatcgcata gaccataagc cgcatcatcc aatttcccaa cgagcgataa gggagtgatt
    10441 tttctttgcc ggtcatttga tccttttaaa tccatcgggg cgtttgaaaa aatgccccga
    10501 tcctttgttt caccgtttgc tgcaggttct cagcactcgc tatagccgca ggcgtagcac
    10561 tttcggcagc cttcttcgtt gatgacggcg gcgacgccgc attcggggca aaggtcgcca
    10621 atgcgctgca caacgggcgg gctggcgggc ggctcttcaa cgggtggggt gaggtcagtg
    10681 atatcgttaa tgaggtattg ctgcacaacg ccgggctttc catcggcttt acccaggcgg
    10741 atagcgcgct catcaaggta ttcctcgagc acctgggcga cgccgtcggg cagggagcgg
    10801 acgcggttcg ggccaaaacc aagcgaacgc ccgccgccga tgcccagcag ctggcgggtg
    10861 aggtcggtga ggcgctgaga gggctcaatc ggcgaagcca ggcgcagcac gtaagagatg
    10921 aggcggccga tggcctcgct gacggcggcg gtatcagagc cggctttggc ggtgttgatg
    10981 aagacctcga aaggctgggc ggcgtcgttc tcgttaatgg taatgaaagc cttgcccagc
    11041 ggggtttcaa tggagaaggt gcgtccgatc agagaacgcg gtctgggctt tttggattct
    11101 tgccacagtt taatttggtc cggcatggcg tcatcagcgg caggggcttt tttactggcg
    11161 gtggcgttgg tttcgagcac cacctcttcg cgtgaaccag tgacgtaaac ggtgatgccc
    11221 ttgcagttaa gttcccaggc gcgcaggtag gcggtggcga cgtcttcctc ggtggcgttc
    11281 tcagggaagt tgatggtttt ggagagcgaa ttgtccacaa aggcttgcag cgctgcctgc
    11341 atgcgcacat gttcctcggc gctgatatca gcgctgacaa caaaggtgtt cttgaccatc
    11401 tcggggactg acttgatatg ctggcaggag ccatctttca tcacctggtc gtagatatcc
    11461 tgacggtcca tctcgctgac acccgcttca agcaaggcct gttcgaatgc aggcgaggtg
    11521 taagtgagtt taaggtcttt gccgttgtca ttaacgtggc ggatgtacgc cagggcgaaa
    11581 accggctcgc agccgtatcc ttcacaaccg gcgacagtgg cgatggtgcc ggtgggggcc
    11641 acggtggttt gcgcggcatt gcggattccg taacgccgaa taccctcgct gatctcgctc
    11701 caatcaatgg tggggcgccc ccagtactgc tgggtttcga tgagtgacac gggcggctgc
    11761 cagacaaggt tttcggggtc gtagatgctg cccttgatgg aggggaaggg gccgcgctca
    11821 cgcgccagtt cgatgctggt tttcatggcg tggtagcgca caaattccat cacctgagag
    11881 gcgaaatcct cggcggcggg cgaaccgtaa cgcactccca cgtgatacat caggtcagcc
    11941 aggcccatga tgcccagccc gatacggcgg ttgcgcatgg cggcctcttt gagctgtggt
    12001 acagccggca cgtaggcgtt ggcatcaacg acgtcatcta agaagcgagt ggccagcaca
    12061 gtattctcgc gtagcttgat ccaatctacg ctgtggtcgg gcccaaggtg ctgggcgaga
    12121 ttgatggagc ccaggcagca gttttcgtat ggaccgagcc attgctctcc gcaggggtta
    12181 gtggcttcca gttggtaaag gtgggggacg gggttggcgc ggttggcggc gtctaaaaag
    12241 agcagcccgg gctcgccgtt atgatgtgcc tgcttgacaa tgcgctgaaa gaggtcgcgc
    12301 gcgcgaatgg tcttataaat ccggaagggg atgcccgcat cttccgcctg ttcaaaggtg
    12361 ccgatgaact ttttcgcgcg cgggtctgaa aggtcaggaa agcgcaggtt ccattccaca
    12421 tcgtcttcca cggcgcgcat gaaggcgtcg gtaatgccaa cggaaatatt aaagttggta
    12481 atctggttct cgttggtctt acaagcaata aactcttcga tatccggatg gtccacgcgc
    12541 aacacggcca tgttggcgcc gcggcgggtg ccgccctggg caacttcgcc aaaggcgtgg
    12601 tcataaacgc gtaaaaaacc caccgggccg gtagcctgac cggctgaagt tttcacatga
    12661 gagcccttag ggcgcaggcg cgaaaaagaa aagccattgc cgccgccggt ttgctggatg
    12721 agggcggcat cgcgcagggt ctggaagata ccggcggagt cgcgacccat atcatcattg
    12781 atgggaagga cgaagcaggc ggcaagctgc cccagggggg tgccagcacc ggtaaaagtg
    12841 ggagaattcg ggaagaaagc cttggaactg agcaggatgt agtatcgctg cgcgagggcg
    12901 tcaacatcgg cggaccagcg ggtttcaatg ttcgcgatgg tggaggctac acgccagaac
    12961 atctcttcga cggtttcagc tggggagcca tcgggattgc ggcgcacgta acgtttgagc
    13021 agcacctggc gggcgttgtc tgagagtgtt acggtgggca ggttatttgg aatcccgccc
    13081 aggtcgagga gtgatttttg ggcagtgcgc ggtggtgtgt ccatgtagtc tcctaggagg
    13141 tcagtaatcg gtcaatttct ttacggatag actgcaggtc agtaagctgc agatacacga
    13201 tggcgtatcg tatataagcg atctggtcca accgcttgag cccgtcgata accatatctc
    13261 caaccacgcg tgaggagacc tcttcacgtc ccattttttg cagggcggtt tcaatctcgt
    13321 tcaccaggtg gttgatatca tccgcagata cggggcgctt ggcgcaggag atacggatgc
    13381 cttcaaggag tttttcacgg ttgaactgct cacgggtgcc agaccgcttg atgatgagcg
    13441 gggtggcgag gatggggcgt tcataagtgg agaagcgctg ctgacaatgt tcacattcac
    13501 ggcgcctgcg tattccgccg cggctgtcgt gtgtggtgtc aattaccctt gattggtctt
    13561 tctggcaata tggacaaagc atcctaccct ccaaattgac cccatgaaca ttgtgccggt
    13621 acctctgacc ggtggctggg ttgtgatggt cgcctggaaa tgacatcccc catatatagg
    13681 gggagctaca taatcagacc gtacatctag gaagtattct aacatacagc gtatttcgat
    13741 tcaagccaaa aaagggtcaa ttttcttaaa actttcagaa atgataataa aactgattct
    13801 gagaagtgtc atggttgatg aagaaaaaac cgagggaaat acggtaacaa acccaatttg
    13861 atccaatagt tatgttaaaa actggcaaaa atcttcaaat tactaaactc ctccttaaag
    13921 tgagataacc ttaaaaaaag caggtagata aaaagagaga ggagcagcca ggcgcgggca
    13981 atttgttccg attaaatgat tcaccggtgc agcgattaaa tcgcgcagct ggcggggacc
    14041 agccgccagc cgcgaatgaa tgatatgacc gggcagagcg ctgtgcgcgc ccggcatatc
    14101 ttatggagga catggttcaa gcatgcgaag caagcgtgag cctacttctc atccagatga
    14161 atgcgcaaga aagtgggtaa atccaaattc ctggtatcca tcttgacagg aacgaactcg
    14221 ttcaccggcg tattgactgg cgtgacctct ttgatttctg tgttggtctg gaaggcgggc
    14281 ttcctgcttt catcattgac ctttgtgacc ggtttcctca ctatgggcga accatttgcg
    14341 cggtcaaacc cggtggcgat cacggtgatg cgcacatcgt cgcccatatc cgggttgata
    14401 accgcgccga agatgaggtt aacctcggga tgggcggttt ctttaataat tgctgccgcc
    14461 tggttgacct catggagagt gaggttggga ccgccggtga cattaaagag gataccgcgg
    14521 gcgccatcaa tggtgatgtc gagcagacgg ttgaaaatgg ccatttcagc tgcttttatc
    14581 gcgcggtctt caccgctggc gcgccccacc gccatcaagg cagcgccgcc ttctgacatc
    14641 acgttcttaa cgtcggcgaa gtcaagatta ataagaccgg ggatcgtaat cacttcggag
    14701 atgccctgta tgccctgttg caatacttcg tcagccagcc tgaaggactc ctgcagagtg
    14761 gatttgcggt ccaccatttg cagcaggcga tcgttaggaa tgacgatgag ggtgtgtgcc
    14821 tgttctttta ggcgggcaat gccctgctca gccgattgca agcgcttggc gccttcgaag
    14881 gtgaaggggc gggtgaccac gccgatggtg agcgcgccac tatctttggc tatctttgca
    14941 ataatgggcg ctgcgccggt gccggtaccg ccgcccatgc cagcggtgat aaagaccata
    15001 tcgctgcctt tgagcacctg ggtcagctcc tcgtaagatt cctcggcgga tttgcgtcca
    15061 acctcgggat tcccgccggc gcccagacca ttggtggtct tatcaccgat gcgaattttg
    15121 gtgggtgcct ttgacatcaa cagcgcctgg gcatcggtgt tcactgtgat gaactcgatt
    15181 ccctgggctc cctgttctat catgcggttg acggcattgc atcctcctcc gccgacgcca
    15241 atgactttga tgcgggcgaa ggtttcgatg ggagatttac tttctgcttc aaaactgctt
    15301 cttggtgatg cgtattgtgg tgttctgttg gtctccattg tttcatcctc cattttgatt
    15361 ttattctgta gcggtcaaca atgaccgtaa ctcacggaag caatctttcc accagcactt
    15421 tcaatttatc ccaattcagg acagatggct tgaagccgct ggttttaggc ttacgcgggt
    15481 gggtaaccgc gtaagagggc tgggtcatca gctccgacca tttcagcaga ccaacgctgg
    15541 tggaataagc tggtgattgc agctggtcaa ccaacccgac gaggttttcg gggcgggcga
    15601 cgcgcaccgg catgttcagc acgtcggcag caagctggcg gataccagga agctgactgg
    15661 ttccaccggt gaggatcatg cccgctggaa ggagattttc gtacccggaa cggcgcactt
    15721 cctgatgcag cagttgaaag atctcttcga cgcgggcttc gatgatgtaa gccagttcgc
    15781 ggcggctgat ctgcttgggc tggtcatcgc caaaggttct aagggtaaag aactcgcctt
    15841 cgccgacttc gccagtgatg gcgtgcccat actgcttttt gatctgttct gcctgctccg
    15901 ggctgatgtg caaaccaatg gcaatatcat ttgtgatttg gttgcctgcc accggcagca
    15961 cgtcggtgtg ccacacatct ccattgatat aaatggcaag gtcgctggtg ccgccaccga
    16021 tatcgcaaat acaaacgccc atgtcgcgct cgggtttatt gagcaccacc tcgcccgaag
    16081 ccagggggtt gagcacgaat tgcaacacat ccacattgca cccgttgacg cactgccgca
    16141 agttctcaac agtggctgct gcggcggtga tgatgtgagc ctcgacctcc agccggaagc
    16201 cagagaagtt gactggtgat tgaatgcctt cctgtccatc cacggtgaaa ccgcgttgga
    16261 tcacgtgaat gatctcacgg ttatggggca tggcgaccgc catggcagcc tcaagggctc
    16321 ggtccacatc ttcctgagag ataaccccgc cgctgatgcc caccacaccg cgtgagttca
    16381 tggccataac gtgcgaacca gccaggctga ccagggcaga ttcgatttca agcccagatg
    16441 tgcgttgggc tttttcgata gagcgagaga tggattgggt agctgtgctg atatccacca
    16501 cgttgccttt tttgatgccc agggccggct cgatgcctac acccaatatg cgcatatctg
    16561 tgtcgccttc caggcgggcg accagggtgc agatcttggt g
//
LOCUS       DAOTLW010000040        15389 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_4283_length_15389_cov_7.853463, whole genome shotgun sequence.
ACCESSION   DAOTLW010000040 DAOTLW010000000
VERSION     DAOTLW010000040.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15389)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15389)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15389
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4283_length_15389_cov_7.853463"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..5225)
                     /locus_tag="P5516_06940"
     CDS             complement(<1..5225)
                     /locus_tag="P5516_06940"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRT92205.1"
                     /translation="MIKNTKWILTLVTLLFVIGLMLPAKASGEGVRTAEASRLLDEES
                     TGVRFTVTVPVEELELEEIEENGKTYTRVGLPGWSQTAQAGAPSLPLVVEQVGAPLGA
                     EVTLYVVPGQARVEKLSAPVLPVVTQRAEMKPPVEGEPLLPEMVYVVEEDASVYGGGE
                     YPGSLAIVMNDGMVRQQRVLGVVVYPVQYNAQRQELTVYETLEVEVRFGGGGFEGGEA
                     EDSAAYESVLEGELLNYESAQSWRASPLEAEGRMSPLGLTGAGVPWAPPEPGWRVKVR
                     NDGVYRLSYAELVAAGLPVSSLDPRTIKMYNLGKELAIRVVGEGDGEFNSTDYIVFYG
                     EGLDSKYTWDNIYWLTYGGDNGRRMGTRNGTPGAGSTPGWHVSNLHIEENTYYFSNAP
                     GSDDLERYFGAFLYPPSIPSWTKTFTLDAPANAAGQMTISMIGYLANAINPDHHVRVT
                     LNGEQIGDVYWDGVTWSNLSMPLRAGLLKAGENTLVVTAVNDTGVGSDMLYIDAVDVE
                     YANTFTAVGDELWFKNGAAGVYRYRLNGFTTGQVEVYDVTDAENVEQISNLAVSGSNP
                     YMVEFSDVAGGAGQYLAKAAGSYKAVQGIETVDTASNLRGVGNGADHIIITPRAFWEQ
                     AETLRAHRAGQGLRAVKVDLQDVYDEFNYGIASAEAIRDFLEYTYNAWVKPSPSYVVL
                     MGDGHFDAKNYQNLGKVNYIPPYLAFVDPWLGETAADNRYVTFKDRMGNRDAMPDMML
                     GRMAVNSAAEAAAMVNKTIAYEANPAEPWQQQVLAVADNRDSGGLFDLLSDSLLAGHL
                     PAPYTAEKVYLNVTHMTAADAKTAILAGINSGKLLVNYIGHASRTDWATEKLFGVADV
                     KNLANGSKQPIILGMTCYDGYYQMPYLSDYFATAEVVTRGAKFDGAEDKGAVASWSPS
                     GLGVASGHDRLNRGFFDALLTDGVETLGQATYAGKAELWASGTNYDLLETFLLFGDPA
                     LRVNRVPALKVSGNITTPSGGPFSGVEVSTTLANGQIRKATSNVDGKFLFNGLPRGEY
                     VFTPLMTGYNFNPAASAPQVLDDSDLSGVNFTSSLAPIFSISGTVKDYQGQVLDDVTV
                     SIDNGQAPVVTQSDGKYAFGELTLGTYQVSVSRAGYLFPVPSQSVTISGVSRTNINFS
                     GLPLHSISGRITYNGAPLPGVTVDDALGHTALSGVDGTYLFEDLTPEVYTLTPSLEGY
                     AFVPGSRQVTLSAVDVPGMNFSADKLGVNDPPLVLDIPDQQIVEGETFTTINLDDYVV
                     DVDNPVEEIEWTFTGNTDLNVNITNRVATITLPHADWFGSEVITFRATDSDGLYGEDA
                     VTFTVTPVNDAPVMVENIPDQWVIHGAAFAPIELDRYISDVDNSADEINWSVAGDTNL
                     DVTIIDRVATITQREANWSGSETVTFRGTDPEGLFAEQPVNFDVVAVKPTVGQKLTAK
                     RVTFEWSGITNANQYNVQLSLSDTFDKILLNATTSNLRFAYGSDLANGKTYYWRIRPR
                     FGTTWGDWSTVKTSDQKPLEFLSMNPPLAPTLKAPAAGAFLRVKDVDFDWTDVVNADH
                     YQLQISKTTAFKDPNTYYPQPGDQPFTVHDLQDGLYYWRIRAIDEVDVAGGWSATRSF
                     TVDTIAPAAPALIAPLAGAYTYDTTPTLAVKAVPGATWYRYRVVTKAEGEPATCEQNV
                     VLTSDKLNKTSWSTMTPELPYGDTYYWCAQAVDAAENESPWSESEARQITITLQNLPK
                     PGTETMVRTPAFSWFA"
     gene            complement(5702..8956)
                     /locus_tag="P5516_06945"
     CDS             complement(5702..8956)
                     /locus_tag="P5516_06945"
                     /inference="COORDINATES: protein motif:HMM:NF013524.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HRT92206.1"
                     /translation="MFNKGRSILIIWTAVLFVIGLMLPAKASGEGVRTAEASRLLDEE
                     STGVRFTVTVPVEELELEEIEENGKTYTRVGLPGWSQTAQAGAPSLPLVVEQVGAPLG
                     AEVTLYVVPGQARVEKLSAPVLPVVTQRAEMKPPVEGEPLLPEMVYVVEEDASVYGGG
                     EYPGSLAIVMNDGMVRQQRVLGVVVYPVQYNAQRQELTVYETLEVEVRFGGGGFEGGE
                     AEDSAAYESVLEGELLNYESAQSWRASPLEAEGRMSPLGLTGAGVPWAPPEPGWRVKV
                     RNDGVYRLSYAELVAAGLPVSSLDPRTIKMYNLGKELAIRVVGEGDGEFNSTDYIVFY
                     GEGLDSKYTWDNIYWLTYGGDNGRRMGTRNGTPGAGSTPGWHVSNLHIEENTYYFSNA
                     PGSDDLERYFGAFLYPPSIPSWTKTFTLDAPANAAGQMTISMIGYLANAINPDHHVRV
                     TLNGEQIGDVYWDGVTWSNLSMPLRAGLLKAGENTLVVTAVNDTGVGSDMLYIDAVDV
                     EYANTFTAVGDELWFKNGAAGVYRYRLNGFTTGQVEVYDVTDAENVEQISNLAVSGSN
                     PYMVEFSDVAGGAGQYLAKAAGSYKAVQGIETVDTASNLRGVGNGADHIIITPRAFWE
                     QAETLRAHRAGQGLRAVKVDLQDVYDEFNYGIASAEAIRDFLEYTYNAWVKPSPSYVV
                     LMGDGHFDAKNYQNLGKVNYIPPYLAFVDPWLGETAADNRYVTFKDRMGNRDAMPDMM
                     LGRMAVNSAAEAAAMVNNTIAYEANPVGSWQQQVLLVADDPDAGGNFPALSNNLMNCC
                     MARQLSADLVYYGVTHTTQTAAKAAILAGINSGKLLVNYIGHAYKDKWADEDLFGVAD
                     IKNLANGSKQPIILAMACNDGYYHMPYPNKYFATAEVVTRAQGKGAVASWSSTGMGMS
                     NAQEYLNRGFLYAYSTLNTPTVGQATLTGKAFLWASGGNLESLDTFLLFGDPALQFPS
                     APTAVDLSAFNVTSAGKTVTVAWQTENEVDNLGFNVYRASDLKGARVKINSLMIPTGT
                     HPGSMVGSSYSFTDKGADLKGGLKPKQTYYYWLEDLNITGGSELHGPLAVEVLP"
     gene            complement(9315..10223)
                     /locus_tag="P5516_06950"
     CDS             complement(9315..10223)
                     /locus_tag="P5516_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92207.1"
                     /translation="MGCEVCGFKVAELNICIDYGKLPRRFAFPACYREFLLPGENHLK
                     HPAGQKASTARAGETLWLKVVEQGTSVEPGFSKALFNGANWQLWLDARERYIFIAPQE
                     TPSYRIAIERDFSCGEIYGDFSSAEGQKLFPLQNLGIRIFINWFANFGDMVVHASGAV
                     INGKGYFFAGQSGAGKSTLISALVGKPGVTALGEDQVIIRFLRGQFWLFGTPWHTNPT
                     LCSPLGAPLHSGFFLDRDKEEGITALTPVEGVVRLFQTAFIPFYRPAAVERILENCSL
                     LAKQIPFYSLSYRLGTSVLDRILHPQ"
     gene            complement(10521..11609)
                     /locus_tag="P5516_06955"
     CDS             complement(10521..11609)
                     /locus_tag="P5516_06955"
                     /inference="COORDINATES: protein motif:HMM:NF015983.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HRT92208.1"
                     /translation="MECSSLPVIDFSEWTGSLATTMQRERYPLSCTFELIDRCNLRCV
                     HCYINQPAGSVESRSSEMDTAQVMQLLDRIAEAGCLFITLTGGEVLLRPDFAQIYRYA
                     RQRGFLVSVFTNGTLLTPEIADLLAEIPPVAVEITLYGATQETYERVTGVAGSYERCL
                     RGIRLLMERGVKLRLKTFVITINRHELPQMQKLAEQLGVEYRYDGIVWPRLDGNTSPM
                     QYQLSLEQVVDIELSEPEKIREWVALMNNNGKKNRAEKVFICGAGFQSFHIDSAGRMS
                     GCISLRDPSFNLFEMDFNEAWERLGKERELKRQLHTPCVDCTLGNLCDQCPGWSKSVH
                     HDYESQVEFICKLAHLRLERIQQSAIIN"
     gene            complement(11609..11902)
                     /locus_tag="P5516_06960"
     CDS             complement(11609..11902)
                     /locus_tag="P5516_06960"
                     /inference="COORDINATES: protein motif:HMM:NF017239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HRT92209.1"
                     /translation="MAGNAATIERNNDFIFRRIADEGVLVPLYQQENEENCIYTLNGI
                     GAFIWEKLEQPSSVAELRSAIFAEYEADPDTIDADLDRFLVEMKTIGAVKEMP"
     gene            complement(12210..>13488)
                     /locus_tag="P5516_06965"
     CDS             complement(12210..>13488)
                     /locus_tag="P5516_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92210.1"
                     /translation="PLGILNAGDIVYYNITVKNTGSTMVNNVYVWDDIPLHTAYVPGS
                     TEYKLDAGGWAQIPDDLVGDGFPLDADGGVLLGDLAVGSTYYVRFKIEILPGDYEVIT
                     NCEQTFTSGGDLEVCVVNPVATRDWGDLPDSYGTTAATDGPRHSYSGLMLGVYWDLEA
                     QGQPGLGLANGDDLNGFPDDEDGVAVVGNASTWEAGSGQFLVTVTGGPGCLDAWMDFT
                     NDAGSGVGYDGLNNYVDGNFTKAGGGYDTYNGKSEHVIRNLLLTNATNIVNVDYPPMV
                     FNEKSYYFRFRITPPVAGGCEPNILPTGYQAGGEVEDYLFTWGTPTAVDLMDFSASSE
                     DGTVIVTWETASELDNLGFNILRSTSVAGVKVRLNNLMLPTNLPPGSMVGSVYEFTDL
                     DNLERGKVYYYWLEDVDIYGIAHINDPVAVKVK"
     assembly_gap    13489..13588
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<13589..>15389)
                     /locus_tag="P5516_06970"
     CDS             complement(<13589..>15389)
                     /locus_tag="P5516_06970"
                     /inference="COORDINATES: protein motif:HMM:NF036933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92211.1"
                     /translation="AGDVIIPNNAIPLPPGPPTYYVLDRFNTVAYNNQDGNTNWTSNW
                     VESGDNENPASGQILITGNQLRFENTASGNTIDRGLDLSAAGECSTLTFSLGKSSGLN
                     SNDRLQVRMSSGGSYGTIATFDQNEPAGTKTINIAATYRTANARLQFRSQDQLEGGDY
                     WTIDDVQVSYGCAPERDRNAIYFDGRDKVGATHAIAMTRAVWASGSGTLNAFGHEMYA
                     TAEWGKYYESPVGTNTANAGQMFEYSGLSIMASQNGTTVNIDKDANGTFETTITLNEG
                     GSYLVEGISQGARVSADKPLQVVLVTGDIGSTYASRDMNLLPVEQWGNSYWSPVGVVY
                     GSNYTRLFIYNLSSNGSTYITCEKYGATKVTSGLVAPGGVWTTDLVNGQGANCYASTA
                     GGTKTDGKIQMIGTIDTTGTAYDWSFTAYPDNFLTTEGLVGLGLGRDPLSSTNQTENG
                     NPIWVTTTCEGGTYVYVDWDNDGIADPIDTNGDGTAEPGSANGILLNRLQSLRLFEPG
                     KDVEPYDQSGARVWSRTTSGVGHGGTPGCLLALAWGEDPANATAGAPGLDVGTSIPPL
                     RLIEGTKALEVVTDTIPLGILNAGDIVYYNITVK"
BASE COUNT         3148 a         4797 c         3846 g         3498 t
ORIGIN      
        1 gcgaaccagc tgaaggcggg tgttctcacc atcgtttccg taccgggttt cggcagattc
       61 tgaagagtga tggtgatctg acgcgcctct gattctgacc agggcgattc gttctccgcc
      121 gcatcgactg cctgcgcgca ccagtagtag gtgtcgccat agggcagttc cggggtcatc
      181 gtgctccagc tggtcttgtt gagtttatcg ctggtaagca cgacgttctg ttcacaggtc
      241 gctggttcgc cttctgcttt ggtcaccacg cggtagcggt accaggtggc gccaggcacg
      301 gctttcaccg ccagcgtggg agtggtatcg taagtgtagg cgccggctaa cggggcaatc
      361 agcgctggcg cggcaggcgc gatcgtgtct acagtgaaac tacgcgtcgc agaccagccg
      421 cccgccacgt ccacttcatc aatcgctctg atgcgccaat aatatagccc gtcttgcagg
      481 tcatgcaccg taaacggctg gtcacctggt tgcgggtaat aggtgttcgg gtccttaaaa
      541 gcggttgttt tactgatctg cagctggtag tggtcggcat tgaccacatc cgtccagtca
      601 aagtccacgt ctttcacccg caagaaagcc ccagcagccg gcgctttcag cgtgggcgcc
      661 agcggcgggt tcatggaaag gaattccaac ggtttctggt cgctggtttt caccgtagac
      721 caatctcccc aggtggtgcc gaagcgcggg cggattcgcc agtaataggt ttttccattg
      781 gcaaggtcgc tgccgtaggc aaagcgcagg ttcgatgtgg tagcgttcag cagaattttg
      841 tcaaaagtgt ctgacagaga taactggacg ttgtactggt tggcgttcgt gatacccgac
      901 cattcgaagg tcactctttt agcagtcagc ttctgcccca cggtaggttt aaccgcgacg
      961 acgtcaaagt tcaccggttg ctcggcaaac agcccttcag ggtctgtgcc cctgaaagtg
     1021 accgtctcac tgcctgacca gttcgcctcg cgctgggtga tggtggccac gcggtcgatg
     1081 atcgtgacgt cgaggttggt gtcgcctgca acagaccagt ttatctcatc cgcgctatta
     1141 tccacatccg agatataacg atccagttcg atgggcgcaa aagcagcgcc gtgtatcacc
     1201 cattggtccg ggatattctc aaccatcacc ggcgcgtcat tgaccggggt cacggtaaag
     1261 gtcaccgcgt cttcgccata cagcccgtca gaatcagtgg ctctgaaggt gatcacctcc
     1321 gacccgaacc agtcagcgtg cgggagggtg atggtggcga cgcggtttgt gatgttcacg
     1381 ttgaggtcgg tgttccccgt gaacgtccat tcgatttcct caaccgggtt gtcgacatcc
     1441 acgacatagt catccaggtt gatcgtcgtg aaggtttcgc cctcgacgat ctgctggtcg
     1501 gggatatcca gcaccagggg cgggtcattc acccccagct tgtcggcgga aaaattcatc
     1561 ccgggcacat ccaccgcgga aagggtcacc tgccgtgagc ccggtacgaa agcgtatcct
     1621 tccagcgagg gggtgagggt gtagacctca ggggtgaggt cctcgaacag gtatgtgcca
     1681 tccacgccgc tcagcgccgt gtgcccgagc gcgtcatcca cggtcacacc tggcaaaggc
     1741 gcgccgttgt aggtaatacg tccggagatc gaatgcagcg gcagaccgga aaagttgata
     1801 ttggttcggc tcacgccgct gatggtgacc gattggctgg gcacggggaa gagataaccc
     1861 gcccgcgaaa cgctcacctg gtaggtgccc agggtcagtt cgccaaaagc gtacttacca
     1921 tcgctttgcg tgaccacggg tgcctgcccg ttgtcaatgg agacggtaac gtcatcgagc
     1981 acctgcccct ggtagtcctt caccgtgcct gagatggaaa aaatcggcgc caggctggag
     2041 gtgaaattca cgccgctcag gtcgctgtcg tccaacacct gcggagcgga agcggcaggg
     2101 ttaaagttgt agccggtcat aagaggagtg aacacgtact caccgcgcgg cagcccgttg
     2161 aacaggaatt tcccgtcaac gttgctcgtc gccttgcgga tctggccgtt tgccagcgtt
     2221 gtggatacct ccaccccgga gaacggtccg cctgaggggg tggtgatgtt acctgagact
     2281 ttgagcgcgg gaacgcggtt gacgcgcagc gcagggtcgc caaacagcaa gaaggtctcc
     2341 aacaggtcgt aattcgtccc tgaagcccaa agttcagcct tgccggcgta agtcgcctgg
     2401 cccagggttt ccacaccgtc ggtcaacagg gcgtcaaaga agccgcggtt aagccggtca
     2461 tgcccgctgg ccacgcccaa cccgctgggc gaccagctgg ccaccgcgcc tttgtcctcc
     2521 gcgccatcga acttcgcgcc gcgggtgacc acctcggcag tggcgaagta atcgcttagg
     2581 tatggcattt gataataacc atcgtagcag gtcatcccca ggatgatggg ctgcttgctg
     2641 ccatttgcca ggtttttgac atccgccacg ccgaatagtt tttcggtcgc ccaatcggtt
     2701 ctagaagcgt gaccaatata attgaccagc agtttgcccg agttgatgcc ggcgaggatc
     2761 gccgttttag cgtcggctgc cgtcatgtgg gtcacattga ggtacacctt ctctgcggtg
     2821 tacggcgcgg gcaggtgccc ggcgagcagg ctgtcagaaa gcaggtcaaa gagaccgccg
     2881 ctgtcacggt tatcggccac cgccagcacc tgctgctgcc agggctccgc agggttggct
     2941 tcgtaggcga tggtcttgtt caccatcgcc gccgcttccg ccgcgctgtt caccgccatc
     3001 ctgcccagca tcatatccgg catggcgtcg cggttgccca tcctatcctt gaaggtcaca
     3061 tatcggttat ccgccgctgt ctctcccagc cacggatcca caaacgccag gtagggcgga
     3121 atgtagttca ccttcccaag gttttgataa ttcttcgcgt caaaatgccc atcccccatc
     3181 aacaccacgt acgacggcga cggcttcacc cacgcgttgt aggtgtattc caaaaagtcg
     3241 cgaatcgcct ccgccgaagc aatcccataa ttgaattcat cgtacacgtc ctgcaggtcc
     3301 accttcaccg cccgcagccc ctgcccagcg cggtgcgccc gcagcgtctc cgcctgctcc
     3361 cagaacgccc gcggcgtgat gatgatgtga tccgccccgt tccctacccc cctcaggttc
     3421 gaggccgtgt ccaccgtctc tatcccctgc accgccttgt aactgccggc cgccttcgcc
     3481 aggtactgcc cggcaccgcc cgccacgtca ctgaactcca ccatgtaagg gttgctgccg
     3541 ctcaccgcca ggttgctgat ctgctccacg ttctccgcgt ccgtcacatc gtacacttcc
     3601 acctggcccg tggtgaaccc gttcaaccgg tagcggtata ccccagccgc cccattcttg
     3661 aaccacagtt catcgcccac cgcggtgaag gtgttcgcgt actccacatc cactgcatcg
     3721 atatacagca tgtctgagcc cacacccgtg tcattgaccg ccgtcaccac cagcgtgttt
     3781 tcgcccgcct tcagcagccc cgcgcgcagc ggcatgctca ggttgctcca ggtgacgccg
     3841 tcccagtaca catccccgat ctgctccccg ttcagcgtca cccgcacatg atggtcagga
     3901 ttgatggcgt tcgccaggta accaatcatg gaaatggtca tctgccccgc ggcgttggcg
     3961 ggcgcgtcca gcgtgaaggt cttcgtccag ctcggtattg acggcgggta aagaaatgcg
     4021 ccaaagtacc gctccaggtc atcgctgcca ggcgcgttgc tgaaatagta cgtgttttct
     4081 tctatgtgca ggttcgatac gtgccagccc ggcgtgctcc ccgctccagg ggtgccatta
     4141 cgcgtcccca tcctccgccc attatccccc ccataggtca gccagtagat gttgtcccag
     4201 gtgtatttcg aatcaagccc ttctccataa aatacaatgt agtctgtgct gttgaactcc
     4261 ccatcacctt ctccgactac ccgtattgcc agttctttgc ccaggttgta catttttatc
     4321 gtgcggggat ccagtgaact caccggtaac ccggccgcga ccagttcagc gtatgaaagc
     4381 cggtaaaccc cgtcgttcct caccttcacc cgccagccag gttccggcgg cgcccagggt
     4441 acgcccgcgc ccgtcaaccc cagcggcgac atcctcccct ccgcttccag cggcgacgcc
     4501 cgccagctct gcgcgctctc gtagttcagc agttcccctt ccagcacgct ctcgtacgcc
     4561 gcggagtctt ccgcttctcc cccttcaaac cctcccccgc cgaaccttac ctccacttcc
     4621 agcgtctcgt ataccgtcag ttcctgcctc tgcgcgttgt actgcaccgg gtacaccacc
     4681 acccccagta cccgctgctg cctcaccatg ccatcattca tcactatcgc cagcgacccc
     4741 gggtactctc ccccaccgta cacgctcgcg tcctcttcca ccacatacac catctccggc
     4801 agcagcggct ccccttctac tggcggcttc atttccgccc tctgcgtcac caccggcagc
     4861 accggcgcgc tcaacttctc cacccgcgcc tgaccaggta ccacgtataa cgtcacctcc
     4921 gcccccaaag gcgctcccac ctgctccacc accagcggca gcgacggcgc tcccgcctgc
     4981 gcggtctgcg accacccagg cagccctaca cgcgtatagg tcttcccgtt ctcctctatt
     5041 tcttccagct ccagctcttc caccggcacc gtcaccgtga acctcacccc cgtgctctct
     5101 tcatccagca gccggctcgc ttccgctgtc ctcacccctt ctccgcttgc cttcgccggc
     5161 agcattaggc caatcacgaa aagcaaggtg accaaagtga gaatccattt tgtgttcttt
     5221 atcattagag aaatcctccg tcaaacaatt aacaccgata aattcctgaa gcaatcaatg
     5281 cttactgttc tttttgatta ccgattagag cgatcattcc ctccatcatt caaggatgac
     5341 gccctggcaa ttcctccggc cacacccctg cggttcgaat caaaatccag tggctgccag
     5401 cggaaatccc tcaaggctcc ttgaccatct gtcgttctgg tcaagagctt caaaggattc
     5461 aacgccagcg aataccctgg tttaccagcc ttgacctgca aaccgcgagc attccaccat
     5521 taatgattat acaacagttt accgttcaac ctgccacgca ccagcctgtt ttacccagcg
     5581 caacgcaggt tcaggcaggg gctccttcgt cgcaactggc gtcgttgtaa aagcctgatt
     5641 cggcttactc cctgcctgag agggcatctc cgctgttgca caataaccgc gccaagttca
     5701 actacggtaa aacctccacg gcaagcgggc cgtgcagttc actgccgcca gtgatgttca
     5761 ggtcctccag ccaatagtag taggtttgtt tgggctttag tccgccttta agatcagccc
     5821 ccttatccgt aaatgaataa ctggacccca ccatactgcc agggtgggtg cctgtgggga
     5881 tcatcagcga attgattttc acccgcgcgc ctttgagatc gctggcgcgg taaacattaa
     5941 agcccaggtt atccacctca ttttcagtct gccaggcgac ggtgacggtt tttccggctg
     6001 aagtcacatt gaaagcggaa aggtcaacag cagttggggc gctcgggaat tgcagcgcgg
     6061 ggtcgccaaa gagcaaaaaa gtatccagcg actcaaggtt tccaccactg gcccacagga
     6121 aggctttgcc agtaagcgtt gcctgcccca ctgttggtgt atttaaggtg gaataggcgt
     6181 ataagaatcc tctattcaaa tactcttgcg cattggacat ccccattcca gtggatgacc
     6241 agctggccac agcgcctttg ccctgcgcgc gggtgaccac ctcggcagtg gcgaagtatt
     6301 tgtttggata cggcatgtga taatagccat cattgcaggc catcgccagg atgatgggct
     6361 gcttgctgcc gtttgccagg tttttgatat ccgccacgcc gaataagtct tcatcagccc
     6421 atttatcctt ataagcgtgt ccgatgtagt tgaccagcag tttgcccgag ttgatgccgg
     6481 cgaggatcgc cgccttggcg gcggtttgcg tcgtgtgtgt cacgccataa tagaccaggt
     6541 cagcactcaa ctggcgcgcc atgcagcagt tcatcaagtt gttggataat gcggggaaat
     6601 tacctccggc atccgggtca tccgcaacca acagcacctg ctgctgccag gaccccacag
     6661 ggttggcttc gtaggcgatg gtgttgttca ccatcgccgc cgcttccgcc gcgctgttca
     6721 ccgccatcct gcccagcatc atatccggca tggcgtcgcg gttgcccatc ctatccttga
     6781 aggtcacata tcggttatcc gccgctgtct ctcccagcca cggatccaca aacgccaggt
     6841 agggcggaat gtagttcacc ttcccaaggt tttgataatt cttcgcgtca aaatgcccat
     6901 cccccatcaa caccacgtac gacggcgacg gcttcaccca cgcgttgtag gtgtattcca
     6961 aaaagtcgcg aatcgcctcc gccgaagcaa tcccataatt gaattcatcg tacacgtcct
     7021 gcaggtccac cttcaccgcc cgcagcccct gcccagcgcg gtgcgcccgc agcgtctccg
     7081 cctgctccca gaacgcccgc ggcgtgatga tgatgtgatc cgccccgttc cctacccccc
     7141 tcaggttcga ggccgtgtcc accgtctcta tcccctgcac cgccttgtaa ctgccggccg
     7201 ccttcgccag gtactgcccg gcaccgcccg ccacgtcact gaactccacc atgtaagggt
     7261 tgctgccgct caccgccagg ttgctgatct gctccacgtt ctccgcgtcc gtcacatcgt
     7321 acacttccac ctggcccgtg gtgaacccgt tcaaccggta gcggtatacc ccagccgccc
     7381 cattcttgaa ccacagttca tcgcccaccg cggtgaaggt gttcgcgtac tccacatcca
     7441 ctgcatcgat atacagcatg tctgagccca cacccgtgtc attgaccgcc gtcaccacca
     7501 gcgtgttttc gcccgccttc agcagccccg cgcgcagcgg catgctcagg ttgctccagg
     7561 tgacgccgtc ccagtacaca tccccgatct gctccccgtt cagcgtcacc cgcacatgat
     7621 ggtcaggatt gatggcgttc gccaggtaac caatcatgga aatggtcatc tgccccgcgg
     7681 cgttggcggg cgcgtccagc gtgaaggtct tcgtccagct cggtattgac ggcgggtaaa
     7741 gaaatgcgcc aaagtaccgc tccaggtcat cgctgccagg cgcgttgctg aaatagtacg
     7801 tgttttcttc tatgtgcagg ttcgatacgt gccagcccgg cgtgctcccc gctccagggg
     7861 tgccattacg cgtccccatc ctccgcccat tatccccccc ataggtcagc cagtagatgt
     7921 tgtcccaggt gtatttcgaa tcaagccctt ctccataaaa tacaatgtag tctgtgctgt
     7981 tgaactcccc atcaccttct ccgactaccc gtattgccag ttctttgccc aggttgtaca
     8041 tttttatcgt gcggggatcc agtgaactca ccggtaaccc ggccgcgacc agttcagcgt
     8101 atgaaagccg gtaaaccccg tcgttcctca ccttcacccg ccagccaggt tccggcggcg
     8161 cccagggtac gcccgcgccc gtcaacccca gcggcgacat cctcccctcc gcttccagcg
     8221 gcgacgcccg ccagctctgc gcgctctcgt agttcagcag ttccccttcc agcacgctct
     8281 cgtacgccgc ggagtcttcc gcttctcccc cttcaaaccc tcccccgccg aaccttacct
     8341 ccacttccag cgtctcgtat accgtcagtt cctgcctctg cgcgttgtac tgcaccgggt
     8401 acaccaccac ccccagtacc cgctgctgcc tcaccatgcc atcattcatc actatcgcca
     8461 gcgaccccgg gtactctccc ccaccgtaca cgctcgcgtc ctcttccacc acatacacca
     8521 tctccggcag cagcggctcc ccttctactg gcggcttcat ttccgccctc tgcgtcacca
     8581 ccggcagcac cggcgcgctc aacttctcca cccgcgcctg accaggtacc acgtataacg
     8641 tcacctccgc ccccaaaggc gctcccacct gctccaccac cagcggcagc gacggcgctc
     8701 ccgcctgcgc ggtctgcgac cacccaggca gccctacacg cgtataggtc ttcccgttct
     8761 cctctatttc ttccagctcc agctcttcca ccggcaccgt caccgtgaac ctcacccccg
     8821 tgctctcttc atccagcagc cggctcgctt ccgctgtcct caccccttct ccgcttgcct
     8881 tcgccggcag cattaggcca atcacgaaaa gcaccgccgt ccaaataatc aggattgatc
     8941 tacctttatt gaacatgaac ttacctccaa aaaatgaagc catttgctgc gaaaatgatg
     9001 cattcacctg gtgatttaaa aaaacggaat gcgaatgagc tttgtgaacc gcgttctcat
     9061 tctaagagaa atcaatcgcc ctattctgcg tcttgcacat ttgaaaagtt accccccgtc
     9121 gcaaggcaag ttcaaattcc cggtttttca attttttttg cagatttgaa aggtaatcag
     9181 gaagaccacg cagaccagtg ggcgctttcg tacgagccgc tcatcttctt cccgtggctg
     9241 cttgatttcg cgattatcga ttcggttgta tgaaaaacag gtggaatgtt tagatcaggt
     9301 atctaaacca ggtcttattg ggggtgcaga atacggtcta aaacgctggt acccagccgg
     9361 taactcagcg aataaaaagg gatctgtttt gccagcagcg agcaattttc caggatgcgc
     9421 tccactgcag cggggcgata gaagggaatg aaagcggtct ggaagagccg gacaactccc
     9481 tcaaccgggg tcagagccgt gattccctct tctttatcgc ggtccaggaa gaaaccgctg
     9541 tgcaaagggg cgcccagcgg tgaacataaa gttgggttgg tatgccaggg tgtgccaaac
     9601 aaccagaatt gaccacgaag aaagcgaatg atcacctggt cttcccccag cgctgtcacg
     9661 ccaggttttc ccacaagcgc gctgatcagg gtggacttgc ccgcgccaga ttgcccggcg
     9721 aaaaaatacc cctttccatt aatcaccgcg ccggaagcat gcacgaccat atcgccaaaa
     9781 ttggcgaacc agttgatgaa gattctgatg cccaggttct gcagcggaaa taacttctgc
     9841 ccttccgcgc tggaaaaatc gccgtaaatt tcaccgcacg aaaaatccct ctcaatcgca
     9901 atgcgatagg aaggtgtctc ctgcggcgcg atgaaaatgt agcgctcgcg ggcatccaac
     9961 cataactgcc aattggcgcc gttgaacagg gctttgctaa acccgggctc aacggacgta
    10021 ccctgctcga ccactttcaa ccacagtgtc tcccctgctc tcgctgtgga tgccttttgc
    10081 ccggcagggt gcttcaagtg gttctcgcct ggcagtaaaa actcacgata gcacgccggg
    10141 aaggcgaagc gccgtggcaa ttttccataa tctatgcata tgtttagctc agcaaccttg
    10201 aaaccacaca cttcacagcc cattctaata aacaccatcc ttattcttaa aacaagaccc
    10261 cctaaataca ttagagggtc accttgcgcc gttatcaggc gcagttgttg agcaggctac
    10321 ttttttccag gcgaatcgaa catcagagca agattacgta aggcaaaggc tacccggggc
    10381 tttacagttt aagggaaact ccagcctgtc cccgctggtg ctcgtgcggc aagtgcctaa
    10441 aaccgaagcg ctgatattca gacgaacctt cttgactgcc ggtgattgat aagttttttt
    10501 attcataatg cctttgctcc ttagttgatt atagcagatt gttgaatacg ttcaagtctc
    10561 aaatgcgcca acttgcagat aaattccacc tggctttcat aatcatggtg aaccgatttt
    10621 gaccaaccgg gacactggtc gcacaaattg ccaagcgtgc aatccacaca gggggtatga
    10681 agctgtcgtt tcaactctct ttcttttccc aaccgttccc aggcttcatt aaagtccatc
    10741 tcaaacaggt taaaagatgg gtcacgcaat gaaatgcaac cgctcatcct gcccgcgctg
    10801 tcaatatgga aggattgaaa acctgcgcca caaatgaata ccttctctgc gcggttcttt
    10861 ttaccgttgt tattcatcaa tgcgacccat tcgcgtatct tttcaggttc agataattcg
    10921 atatccacaa cctgctcgag cgaaagctgg tattgcattg ggctggtatt tccgtccagg
    10981 cgcggccaga cgataccatc ataacggtat tcaaccccca gttgttctgc cagcttctgc
    11041 atctgcggca gttcgtgccg gttgatggta atgacaaagg tctttaagcg tagcttcacc
    11101 ccccgttcca ttaatagcct gatgccgcgg aggcagcgct cataagaacc cgcaaccccg
    11161 gttacccgtt cataggtctc ctgggtggcg ccgtataaag taatttccac agcgacaggc
    11221 ggtatctcag ccagcaggtc ggcaatttca ggcgtcagaa gggtgccatt agtaaacacc
    11281 gaaacgagaa atccgcgctg cctggcgtaa cggtaaattt gcgcgaaatc aggtcggagt
    11341 aatacctctc cgccggtgag tgtgatgaat aaacagccag cttcggcaat cctatccaac
    11401 aactgcatca cttgcgcggt atccatttcc gacgaccgtg attccacgct gcccgccggc
    11461 tggttgatat aacaatgcac acacctgagg ttgcatctgt cgatcaattc gaaggtgcag
    11521 ctgagcggat accgttctcg ctgcatggtt gttgccagcg agcctgtcca ttcgctaaaa
    11581 tctattactg gcagcgatga gcattccatt atggcatctc ttttacggcg ccgatggttt
    11641 tcatttcgac caaaaaacgg tcaagatcag cgtcaatggt gtcagggtca gcctcatact
    11701 ccgcgaaaat cgcggagcgg agttccgcga ccgaactcgg ttgctccagc ttttcccaga
    11761 tgaaagcacc tattccattc aatgtataaa tacaattctc ctcgttttct tgctggtaca
    11821 gaggaaccag aactccttca tccgcgatcc gtcgaaaaat aaaatcgtta ttccgttcaa
    11881 tggtcgctgc gttgccagcc attatgaaag ccctcctcat caataaatta ccagccattc
    11941 gtgataaaac ccctgacgta acataaggct atattatcat attaaaattg agaaaccgct
    12001 aatcgcgtaa tctcgcgtgt tgactggtct gagtgcttga taagcctttt tctttaaccc
    12061 catcggccct ttttcttctc ctgtaagttc agcctgtctc ttctgttcct gctcctggca
    12121 gggtattcgc cctgccagga gccaggaatc attttccttg agcctttcag gccataacag
    12181 gcggtcagca aaaaccgctg tccgttcggt tatttcactt tcaccgccac cgggtcgttg
    12241 atgtgagcga taccgtagat atccacatcc tcaagccagt agtagtagac cttgccccgt
    12301 tcgaggttat ccaggtcagt aaattcgtaa actgaaccga ccatactgcc gggaggcagg
    12361 ttggtgggaa gcatcaggtt attcagcctg accttcactc cagccacaga ggtggagcgc
    12421 aggatattga acccgagatt gtccaactcg ctggcggttt cccaggtcac tatcacagtt
    12481 ccatcctcgc ttgaagcgct gaagtccatc aaatctaccg cggtcggcgt tccccaggtg
    12541 aagaggtaat cttccacctc tccgcccgcc tggtaaccag tgggtagaat attcggttcg
    12601 caaccgccgg ccaccggtgg agtgatgcgg aagcggaagt agtagctctt ctcattaaag
    12661 accatgggcg ggtaatcgac attcacgata ttggtggcgt tagtgagcaa cagattgcgg
    12721 atcacatgct ctgattttcc gttataggta tcgtatccgc cgccggcttt tgtgaagttg
    12781 ccatcaacgt aattgttcag cccatcgtag cccacgccgg agcccgcgtc gttggtaaag
    12841 tccatccagg cgtccaagca acccggcccg cctgtgacgg taacaaggaa ttgaccgctg
    12901 ccggcttccc aggttgaggc gttgcccaca acagccacgc cgtcctcgtc atccgggaaa
    12961 ccattgaggt catcgccatt ggccaatcca agccctggct gcccctgcgc ttccaggtcc
    13021 cagtacactc ccagcatcaa cccgctgtaa gaatggcgcg ggccgtcagt ggcggcggtg
    13081 gtgccgtaag aatcaggcag gtcaccccaa tcgcgtgtgg ctaccgggtt caccacgcaa
    13141 acttccaggt ctccaccgct ggtgaaggtt tgttcacagt tagtgatcac ttcgtaatcg
    13201 cctggcaata tttcgatctt gaagcgcaca taatatgtgc tgcctacagc caggtcgccc
    13261 agcagaaccc ctccatccgc atccaacggg aagccgtcgc ccaccaggtc atccggtatc
    13321 tgagcccagc ctccagcgtc cagtttatat tccgtcgaac ccggtacata cgcggtgtgc
    13381 aaggggatat catcccacac atacacattg tttaccatgg tggaaccggt gttcttgacg
    13441 gtgatgttgt aatacacgat atctccggcg ttgagaatgc ccaacggann nnnnnnnnnn
    13501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    13561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnct tgacggtgat gttgtaatac acgatatctc
    13621 cggcgttgag aatgcccaac ggaatggtgt ccgtcacgac ttccagggcc ttggtgcctt
    13681 cgatcaggcg cagcggtggg atggaagtac cgacatccag ccccggtgct cctgcagtgg
    13741 cgttcgcggg gtcttctccc caagccagcg ccagcaggca gcccggcgtg ccgccatgac
    13801 caacgccgct ggttgtacgg ctccaaaccc tggcgccgct ttggtcatat ggttcaacat
    13861 ctttgcctgg ttcgaataac cgcaaggact gcaggcggtt gagcaggatg ccgtttgcag
    13921 agcctggttc cgctgttccg tcgccgttcg tgtcgatcgg gtccgcgatg ccgtcattgt
    13981 cccaatccac gtacacataa gtcccgcctt cgcaggtggt tgtgacccag atggggttgc
    14041 cgttctccgt ctggttggtt gaggataagg gatcccgtcc caggcccaga cccaccagtc
    14101 cttcggtggt caggaagtta tcagggtagg ctgtgaagct ccaatcataa gccgtgcccg
    14161 tcgtatcaat ggtgccgatc atctggatct tcccatcggt ttttgtccca cccgctgtgg
    14221 aggcgtagca gttagcgccc tgaccgttaa ccagatcggt ggtccaaaca cctcccggtg
    14281 cgaccagccc tgatgtgacc ttggtagcgc catatttctc acaggtgatg taggtgctgc
    14341 cgttgctgct cagattataa atgaacaggc gggtataatt actgccgtac acaacgccta
    14401 ccgggctcca ataggaattg ccccactgtt ccactggcag caggttcata tcacgcgaag
    14461 cgtaagtgga accaatatcg ccagtgacca gaaccacctg caagggttta tcagcgctca
    14521 ctctcgcccc ctgggatatg ccctccacca ggtaactgcc gccttcatta agggttatgg
    14581 tcgtttcgaa ggtgccgttc gcgtctttat cgatgttcac cgtggtgcca ttttgcgaag
    14641 ccatgatgga taaaccggaa tattcaaaca tctgaccggc gttagcggta ttggtgccca
    14701 cgggagactc ataatatttc ccccattccg cggtggcgta catttcatgc ccgaaagcgt
    14761 tgagcgtgcc cgatccagaa gcccagacag cgcgggtcat ggcgatggcg tgggtcgcgc
    14821 cgaccttatc acgcccgtca aagtaaatag cgttgcgatc ccgttcaggc gcgcagccgt
    14881 aagagacctg cacgtcatcg atcgtccagt aatcgcctcc ctcaagctgg tcttgcgatc
    14941 tgaactgcag cctggcgttc gccgttcgat aagtagcggc tatattaatg gttttcgtac
    15001 ccgcgggttc gttttggtca aaagtggcaa tcgtgccgta gcttccaccc gacgacatcc
    15061 gcacctgcaa tcgatcatta ctgtttaaac ctgatgattt ccccaacgag aatgtcaagg
    15121 tagagcattc cccggctgca gaaaggtcca acccgcggtc aatagtgttg cccgaagcag
    15181 tattttcaaa acgcagctgg ttgccagtga tcagaatttg ccctgaagcg ggattctcgt
    15241 tatcgccgct ctctacccag ttgctggtcc agttagtgtt gccgtcttga ttgttgtagg
    15301 caaccgtgtt gaaccggtca agcacgtaat aggtcggtgg acctggcggg agcgggatgg
    15361 cgttgttcgg gatgatcaca tctcccgcg
//
LOCUS       DAOTLW010000041        15141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_4379_length_15141_cov_10.632109, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000041 DAOTLW010000000
VERSION     DAOTLW010000041.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 15141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 15141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15141
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4379_length_15141_cov_10.632109"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..545)
                     /locus_tag="P5516_06975"
     CDS             complement(<1..545)
                     /locus_tag="P5516_06975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92212.1"
                     /translation="MLWRDGNRRVHDLVVYARQRFYSVGMTTNGTQPLDVPTDVLWVS
                     LDGLGETHNQLRGAPVFDSIMANVRASCHPRLFAHVTVNAINADEVPRLLVFLNESFH
                     AITLQFYYPYGQKDELFLDFDRREKLLDEVIRLKEKGIRVLNSVAALKALKRNTWTCR
                     DRLIDNANPDGTVQQGCYLRG"
     gene            complement(951..1799)
                     /locus_tag="P5516_06980"
     CDS             complement(951..1799)
                     /locus_tag="P5516_06980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRT92213.1"
                     /translation="MIAVITDSTCDIPDPLLRDLGIEVVPQYVIWGDKQYRDRLDIEP
                     AAFYQRLSTEVEKPRSSQPGVMDFQQAYERAANNGADELVVVTISSTLSGTYENARRA
                     AQDFSLPVTVVDSTGTSMGMGWQVLAAARAARSGMDTRGILEKIDHVRRRITQIAGLD
                     TLEYLSKGGRIGAAVKWAGTMLKVKPLVAFNVLTNLVEPVGLVRTYTSMVETLYRKFF
                     ERVKGIANLRVAVLHGGARAQAEALAERVRAEYHPLELFVNSTGPVVGLHTGPGALGL
                     CGYMDE"
     gene            complement(1839..3098)
                     /locus_tag="P5516_06985"
     CDS             complement(1839..3098)
                     /locus_tag="P5516_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92214.1"
                     /translation="MAVVEKSSANKPSVKNLPRNIWAVGFTSMFMDISSEMVMNILPL
                     FLSNVLGVQTSIIGLIDGIAEATASILKLFSGWLSDRLGGRKWLAVAGYGLSALVKPF
                     FYIANSWGLVAGVRWADRVGKGIRTAPRDALVADSVTPDVRGLAFGFHRAMDTAGAFI
                     GILIAALVVWLTQKSDVLLARSTFQIIVLASLLPALLAVLSLVVGAQDVAVQGQRAAP
                     KFSLRNMGKPFTIFMVIVSLFTLGNSADAFLVLRAQNLGVSVLGILIMLAAFNLIYTL
                     VSTPAGSLSDRIGRRRLIIGGWLVYAVIYFGFALARSSWQVWVLYALYGVYYGLAYGS
                     AHALVADLVPENLRGTAYGTYNAVIGLLAFPASFIAGVLWQGIGAWTGLGPSAPFFFG
                     GLLALLAALLMLFWMPAIHTVEEKVLH"
     gene            complement(3134..4087)
                     /locus_tag="P5516_06990"
     CDS             complement(3134..4087)
                     /locus_tag="P5516_06990"
                     /inference="COORDINATES: protein motif:HMM:NF015951.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HRT92215.1"
                     /translation="MGDRYKNTSLHWAKRLIIAFHANQLLQEMIFMSEVTPTTRKTAS
                     IGDWLLRLIKGVIIGIGFITPGLSGGVLAVVFGLYEPLMRFIANLKEKFIKNLLFFIP
                     VGIGGIIGVVAFSAAVDWAFTHYAAPFIWLFIGFIAGTFPSLFKTAGKQGRKAWHWLL
                     LAVIAAGMFLMMKWMESIKNVTLAPSFLNWLMSGALIGLGVVVPGMSPSNFLIYLGLY
                     QPMANGIKNLDLGVILPLALGLILCVLVFAKLVSWLFNKAYTVMYHLILGVVVGSTLA
                     IIPAGVSGWTIALCAVLFLLGFAASFALAKVDEKHPHEQII"
     gene            complement(4359..4970)
                     /locus_tag="P5516_06995"
     CDS             complement(4359..4970)
                     /locus_tag="P5516_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT92216.1"
                     /translation="MTQHQAGEPNAENRPEPGHFRLLAPFYEHFIQPKAPDRILAHLG
                     LPQPGGVVLDAGGGTGRVAQFFAGKAAAVVVADQTFAMLREARKKEGLTGICALTEEM
                     PFRNEGFCCIIMVDALHHVADQEATIRELWRLLKPGGRIIIEEPDIRRFEVKLLALIE
                     KLVLMRSRFLSPGQVTALFNFPGASSRVEVAGWTAWIIIEKTA"
     gene            complement(4967..6751)
                     /gene="thrS"
                     /locus_tag="P5516_07000"
     CDS             complement(4967..6751)
                     /gene="thrS"
                     /locus_tag="P5516_07000"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HRT92217.1"
                     /translation="MARQKEHYEDSQLYRIRHSTAHIMAQAVLEKFPEGKVAIGPAIE
                     DGFYYDFDLPRPLTPEDLEGIEKRMRGIIQSGVNFERKVVSADEAKVLFRNQPFKMEL
                     IEGLESGEMDDDGNPIDEKPEISIYSQGGFTDLCRGPHVTNSREINPAAIKLLNVAGA
                     YWRGDEKRPMLQRIYGTAWSKPEELKDYLWRLEEAKKRDHRKIGQELDLYSVNDEIGA
                     GLILWHPKGGRIRKLIENFWSEEHEANGYDFVYTPHIGKAQLWETSGHLGFYKDNMYS
                     PVDIEGQQYFLKPMNCPFHLHIFKNSLRSYRDLPLRYAEEGTVYRYERSGVLHGLMRV
                     RGFTQDDAHHFCRPDQMPAEIDFVLNFSLNILRSFGFSDIHAYLSTRPEKSVGDPAQW
                     EAAEAALEDSLNRSGLDYDVDRGGGAFYGPKIDLKVEDAIGREWQLSTIQFDFNEPER
                     FDLYYIGEDGQPHRPYMIHRALLGSLERFFGVLIEHYAGAFPVWLSPTQAAIIPIADR
                     HLDYARKVEAELRKAGLRVMVDDRPERMNAKIRDAQNQKIPYMLVVGDKEEESGQVAL
                     RLRNGENPGPLLLADFLTLAKSDIAAKK"
     misc_binding    complement(6793..7027)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(7206..7913)
                     /gene="prxU"
                     /locus_tag="P5516_07005"
     CDS             complement(7206..7913)
                     /gene="prxU"
                     /locus_tag="P5516_07005"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558502.1"
                     /note="Most members of this family contain a
                     selenocysteine; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(7665..7667),aa:Sec)
                     /transl_table=11
                     /product="thioredoxin-dependent peroxiredoxin"
                     /protein_id="HRT92218.1"
                     /translation="MAENKDGCVKPVKGPISAASETPSAAPVFQKEVSMVQAKVGKPA
                     PDFELTAFHQGGFKNITLSDYKGKWIVICFYPGDFTFVUPTELTAVAVKYKELQALGV
                     EVLAISTDSRFSHKIWQEEELSKMVEGGVPFPLLSDAGGRVGTIYGVYDETAGVDIRG
                     RFLIDPDFNIRAMEVLTPEVGRNVSELIRQVQAFQHVVATGEVTPSGWVPGKPTLTPG
                     PDLAGKVWKVWKPDQAF"
     gene            complement(8091..10997)
                     /locus_tag="P5516_07010"
     CDS             complement(8091..10997)
                     /locus_tag="P5516_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HRT92219.1"
                     /translation="MNPAQKSVQLTINGEKKALLVTPEETLLDALRAASYFSVKFGCG
                     SGECGSCTVIMNGKAVRSCTIKAMEAEGSQIITLEGLSKNGELDPIQQAFIETGAIQC
                     GFCSPALMLSAKALLDHQPEPSEKEIRNALNPVLCRCTGYVRGVNAVQRAAAIKRGEK
                     VAPFNHISLPLPDDLAQIEIPAEFFRADGNRDPLPPLVYTPASMPAARVVGKSEIKVD
                     ARKLALGKPVFTDDIHLPGMLYAAMLTSPHAHARIKHIDASRARALPGVHAVLTHHDV
                     PRVKYASGGQSYPQPLPYDQVSLDDKVRFVGDRVAVVAAESLEIANEALKLIDVEYEV
                     LPAVVDMEAAMQPGAPVIHDEPDTEGIYNRERNIVYHIEAEVGDVDKAFAESDHVFEG
                     EYHTQKQQHVHLEPHVAISYFDEDERMVIRTSTQVPFHTRRIIAPLIGLPLKKIRIIK
                     PRIGGGFGNKQEVLIEDLVAHLTMITKRPVRMEYTREQEFTSSRSRHATTIRYKIGVK
                     GDKVTAASLYLIGDTGAYATHGLTVTMVGGFKGLTLYNSPNSRFICDVVYTNTVVAGA
                     YRGYGSMQCQYGIEVLMSEIADRLGLDVVDFKKKNWIKVGEEMYLSQQLGEGRTGVRQ
                     ELVTSAMEQCVEIGRRVTDFDAKRAAYRDQTGRYRRGIGMAVVMHGSSVANLDMAAAT
                     LKMNDDGSFNLLMGATDLGTGSDTILAQIAAEVLNTDVDDIIVYSSDTDFTPFDKGAY
                     ASSTAYLSGEAVRKTAVNIKNQLLEHAGKMMGVKDLNTLRLEYKKVVAADGRQVSFEE
                     IGLSSLHQMAQHQIMATESHVSPVSPPPTAAQFVEIVVDTETGKVDTERLVMVVDCGR
                     VINPVTASGQVEGGMAQALGFALTEDMLYSAEGQPLNPNLVTYRVPRAVEMPVMDVIF
                     VQTDEPTGPYGVKSVAEISIDGVAPAMASAIHNAIGVWLRDLPYTKEKVKKAIG"
     gene            complement(11340..12068)
                     /locus_tag="P5516_07015"
     CDS             complement(11340..12068)
                     /locus_tag="P5516_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HRT92220.1"
                     /translation="MSIYTDLLALLPKEPVPLRSVLVGVHWTLVCSKHSGLGSTLVNY
                     GPHGHAPMRDVGCLLEKSAQELAGWIESDNLLEASLGIAAYNSLIDLDDSKLKEGNAS
                     EVLRCECPGKNLAVIGHFPFITKLRDLAANLWVIEKDPHGDDFPEEAAQEFLPSADVV
                     AITGTAFINHTIERLLSLCRPGVPVMILGPSTPLVPLLFDYGVTYLSGSRIIDEAAAR
                     LTISQGAGFPQVRGVQVVNMVKDA"
     gene            complement(12089..12934)
                     /locus_tag="P5516_07020"
     CDS             complement(12089..12934)
                     /locus_tag="P5516_07020"
                     /inference="COORDINATES: protein motif:HMM:NF014582.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HRT92221.1"
                     /translation="MIFGQSPLLNILLLSLMASLGTGLGGLIAIIRRPGRRSYGLLMG
                     ITAGVMISLAFLELVAEAWKQAGYITATIGFATGAIFMFLLDHFAPHIRFGEKEVRGE
                     PPCAEPRQRMGPRPGGRGFGRLYRQGRMYDYKLINTGLLLAVGITLHNLPEGISIGAG
                     YLHNPKFGLFIAVAILLHNIPEGIATALPLCKGGVCRWDAFRVAFLSGLAEPVGALLA
                     SLFLVNFQGLVPAALAFAGGVMVFITLDEMIPTAREYGHEHYTAIGIILGSIFVFLLS
                     GIFGV"
     gene            complement(13030..13473)
                     /locus_tag="P5516_07025"
     CDS             complement(13030..13473)
                     /locus_tag="P5516_07025"
                     /inference="COORDINATES: protein motif:HMM:NF024986.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HRT92222.1"
                     /translation="MKIIYEDLQRIIQAHARFLAGECDGTRANLEGIDLYGKDLANVN
                     LAGANLKSTNLKRSILWDANLEEADLEQAAFKHAILQGANLRKANLRGAGLMGVNLIG
                     ANLSGADLTGADLDCAVLQGALYDKTTQWPEGFDPLSAGAICQDE"
     gene            complement(13546..14586)
                     /locus_tag="P5516_07030"
     CDS             complement(13546..14586)
                     /locus_tag="P5516_07030"
                     /inference="COORDINATES: protein motif:HMM:NF014620.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HRT92223.1"
                     /translation="MTLPTDPDQGTEHTTTEMMVGQRLRKLRVSKGLSLRSLADLSGL
                     NINTLSLVENAKTSTSVGTLQQLAAALSVPLSAFFVEETIEKQVVFTPAHPQPTTAFN
                     HTQLVYLAQNLSRKTIQPYLVTLKAGMGSGNNMIVHTGHEFVYCIEGQAQFTIDNEVY
                     PLKTGDSLIFEAHLPHAWENNQPALARLLMILAPADAAEEAGSRHVSLEKLQKEKTMK
                     IAVITEDGKKISQHFGRAPYFLVLTIEDGKVTHREMREKPGHGHFGGRAEETHGHRHG
                     FDEGSHLKHKSMAETISDCKVVLCGGMGMGAYDSMRRLEITPIVTDLKDIDEAVKLYL
                     EGKLVDHPELLH"
     gene            complement(14591..15025)
                     /locus_tag="P5516_07035"
     CDS             complement(14591..15025)
                     /locus_tag="P5516_07035"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014608.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRT92224.1"
                     /translation="MPNKNVSVEAVLLEGYKIEVKSRQHVSIVDQPPAGGGGDAGPTP
                     LEYLFVSLAGCVATIGQIIAKQQQLPLCGISVKIDGDLDTDVLMGKRSDVRAGFEGIR
                     VKVAIDGDMTQQEKEQFVRDIDARCPISDNILNQTPIKFAVE"
BASE COUNT         3553 a         4275 c         3857 g         3456 t
ORIGIN      
        1 ctgccgcgca ggtaacaacc ctgctgtaca gtgccatctg ggttggcatt gtcgatgagc
       61 ctatcacggc aggtccaggt attgcgcttg agtgccttga gcgcggctac tgaattgagc
      121 accctgatcc ctttctcttt cagccggatg acttcatcca ggagtttttc acgcctgtca
      181 aaatcaagga acagctcatc tttttgccca tatgggtaat agaattgcag ggtgatggcg
      241 tggaaagact catttagaaa caccagcagc cgcggaacct catccgcatt gatggcgtta
      301 accgtaacat gggcaaaaag gcgcgggtgg cagctggcgc gcacgttcgc catgatggag
      361 tcaaatactg gcgcgccccg caattgattg tgtgtctcac ccagcccatc caagctcacc
      421 caaagcacgt ccgtgggcac atccagcggt tgggtgccat tggtggtcat gcccacgctg
      481 taaaaacgct gccgcgcgta aaccaccagg tcgtgcacgc gccggtttcc atcgcgccac
      541 aacattggtt ctcctccttc gaaaattaaa acacggttac cgcgcgcgta aagccgatcg
      601 atggtcgcgc aggcttggtc ataatttggc tcagaggttt ttaggaggtg ataagggcat
      661 tggcggcagg tgagattaca acgataagtc aatttatagc tcgccagaaa gggtcgcttt
      721 cgacccagcc agtagtgcaa aaaaatgtag atagcgcgcc tggtcaaccc ttttagatca
      781 gtatgaaaat gaggcatata ggtattgtat caagcccgag cgcctggcgt catgccaaag
      841 gaaagtgtgg gtcagcagta agctgctggc ggatgaactg atccacgcag gcgtcaacct
      901 tggaacccca catggcaatt aacccgtcat gtacctgcgc tttcgttcac tcattcgtcc
      961 atataaccac aaagtcccag cgcgccgggg ccagtatgca gccccaccac cgggcccgtg
     1021 gagttgacga aaagctccaa tgggtgatat tctgcgcgca cccgttcagc cagcgcttca
     1081 gcctgcgccc gcgctccgcc gtgtaacacc gccacgcgca ggttcgcaat ccctttaacc
     1141 cgttcaaaaa attttcggta cagggtttca accatggagg tatacgttcg caccagccca
     1201 acaggctcta ccaggttggt aagcacattg aacgcgacca gcggcttgac cttcaacatg
     1261 gttcccgccc atttaaccgc ggctccgatg cggccgccct tgctcaggta ttcaagcgtg
     1321 tccagtccgg cgatctgggt gattctgcgg cgcacatggt cgatcttctc cagaatgccc
     1381 ctggtgtcca tgcccgatcg cgcagcgcgg gcagccgcca gcacctgcca tcccatgccc
     1441 atcgaagtgc ctgtggaatc caccacggta accggcaaag agaagtcctg cgccgcccgc
     1501 ctggcgttct cgtacgttcc actgagcgtg ctgctgatcg tgaccactac caattcatca
     1561 gcgccattat tagcggctcg ttcataagcc tgctgaaaat ccatcacacc cggttgcgaa
     1621 gagcggggct tttcaacctc tgttgataaa cgctggtaaa acgcagcggg ttcaatatcc
     1681 agccggtcgc ggtattgttt atctccccaa atgacgtatt gcggcaccac ttcaatcccc
     1741 aaatcccgta aaagagggtc aggaatgtca caggtgctgt cggtgatgac cgcgatcatg
     1801 ggactatttt acaccggtaa tgtgataaag attatttatc aatggagtac tttctcttcc
     1861 accgtatgaa ttgcgggcat ccaaaagagc atcaacaggg ctgccaacaa ggccagcaag
     1921 ccaccgaaaa agaacggcgc tgacgggcca agccccgtcc aggcgccgat gccctgccaa
     1981 aggacgccgg caatgaaaga tgcggggaac gccaacagac cgatgaccgc gttgtacgtg
     2041 ccgtaggctg ttccgcgtaa gttctcagga acaaggtcag ccaccaaggc atgagccgaa
     2101 ccgtatgcca gcccataata aaccccgtac agcgcataga gcacccacac ctgccagctg
     2161 gaacgcgcca gcgcaaagcc aaaataaatg accgcgtaca ccagccagcc gccaatgatg
     2221 agccgtctcc tgccaatgcg gtcagatagc gaacccgcag gagtggatac caacgtgtaa
     2281 ataaggttaa acgctgccag catgatgaga attcccagca ctgaaacgcc caggttctgc
     2341 gcgcgcagca ccaaaaaagc atctgcagag tttcccaggg tgaacaggct gacaatgacc
     2401 atgaaaatgg taaagggttt gcccatattg cgcagcgaga atttcggcgc ggcgcgctgc
     2461 ccctgaaccg ccacgtcttg ggcgccgacc accagcgaaa gcaccgccag gagcgccggc
     2521 agcaggctgg ccagaacgat aatctgaaaa gtcgaacgtg ccaaaagcac gtcgcttttt
     2581 tgcgtcagcc acaccaccag tgcggcgatg aggataccaa taaaggcgcc ggcagtatcc
     2641 atggcgcggt gaaaaccaaa cgccaggccg cgaacatcag gggtgacaga atctgccacc
     2701 agcgcgtcac gcggggcagt acggataccc ttgcccacgc ggtcagccca gcgcacccct
     2761 gccaccagcc cccagctgtt ggcaatgtag aagaatggct tcaccagggc agaaagacca
     2821 taccccgcca ccgccagcca tttgcgccca cccaggcggt cagaaagcca accagagaac
     2881 aacttgagga tactggcagt ggcttcagca atgccatcaa tgagcccgat gatgctggtc
     2941 tgcactccca gcacgttgga aagaaaaagc ggcaggatat tcatgaccat ttcgctggaa
     3001 atatccatga acatgctggt aaaacccact gcccatatgt tacgcggtag gtttttaact
     3061 gaaggcttgt tagcagaact cttttcaaca actgccatcc cgggtctcac tcttcctgaa
     3121 aaggggaaaa taattagatg atctgttcgt gcggatgttt ttcatccacc ttggccaacg
     3181 cgaaggatgc agcaaaaccg agcaggaaca acaccgcgca cagcgcgatg gtccatccgc
     3241 tcacccctgc cgggatgatc gccagcgttg aacccaccac cacacccaga atcaggtgat
     3301 acatcacggt ataggctttg ttgaacaacc acgagaccag tttagcaaaa accaacacgc
     3361 acaggatcag cccaagcgcg agagggagaa tgacccccag gtccaggttt ttaatgccgt
     3421 tggccatcgg ttgatataaa cccaggtaga tgaggaagtt agaaggactc attccaggca
     3481 ccaccacacc cagcccgata agcgcgccgc tcatcagcca gttgaggaag ctcggcgcca
     3541 gggtgacgtt cttgatgctt tccatccatt tcatcatcaa gaacataccg gcggctatca
     3601 ccgccagcag gagccagtgc caggctttgc gcccttgctt gccggctgtt ttaaagagcg
     3661 aagggaaagt accggcgatg aaaccgataa aaagccagat gaacggcgcg gcgtaatgcg
     3721 tgaaagccca gtccaccgcc gccgagaacg ctaccacacc gatgatcccg ccaataccca
     3781 ccgggatgaa gaaaagcagg ttcttgatga atttttcctt caggttggcg atgaaacgca
     3841 tcaacggttc ataaagacca aataccaccg ccagcacacc gcctgaaaga cccggggtga
     3901 tgaagccgat gccgatgatg acgcctttta tcaggcgcag cagccagtct ccaatcgaag
     3961 cggttttcct ggtcgtgggt gtaacctctg acataaaaat catctcctgt aaaagttgat
     4021 tcgcatgaaa tgctattata aggcgctttg cccaatgtag tgaggtgttt ttataccgat
     4081 ctcccaatgc cagtgccctt cgcgctgtgt ggtcaatgaa caggaaggtt cactccctcg
     4141 cccagccctc tgctgccagt acaggcaggg cggcagaaca ggcaaaaaaa aaaacagcag
     4201 gataatcgaa gcgcacaccc cagagttcag agcccgctga aatgtgcgca agccacgcca
     4261 tgcagccatc catagaggtg gataaagccc ttgtacgcgt cagcctaaaa gtccttccct
     4321 cgtcaggatg gactttttaa gcgccattca tgcatgggtt atgcggtctt ttcgatgatg
     4381 atccaggcag tccagcccgc gacttcaaca cgtgaggaag ccccagggaa attaaagagc
     4441 gcagtcacct gacctggtga gagaaatcgg ctgcgcatca gcaccagctt ttctatcagc
     4501 gccagcagtt tcacctcaaa acggcggatg tcaggttctt caataataat gcgtccgccc
     4561 ggtttaagaa ggcgccataa ctcacggatg gtagcctcct ggtctgccac gtgatgcagc
     4621 gcatcgacca tgatgatgca gcagaaacct tcatttctaa agggcatttc ttcggtcagc
     4681 gcacatatcc cggtcaaccc ctcttttttt cgcgcttccc gcagcatcgc gaaggtctgg
     4741 tctgccacca ccacagccgc cgcttttccc gcgaagaact gggcaacacg cccggtgccg
     4801 ccgccggcat ccagaactac accgcctggc tgcggcaacc ccaggtgggc aaggatcctg
     4861 tcaggcgcct tcggttggat aaaatgctcg tagaaaggag ccagcagcct gaaatgaccg
     4921 ggttccggcc tgttttccgc gtttggctcc cctgcctggt gttgagtcat tttttggcgg
     4981 cgatatctga tttcgccagc gtaagaaaat ctgccagcag aagtggacca gggttctcgc
     5041 cattgcgcaa gcgcagcgcc acctgcccgc tctcctcttc tttgtcgccg acgaccagca
     5101 tgtaagggat cttctggttc tgggcatcgc gaattttggc gttcatgcgc tcgggtcggt
     5161 cgtccaccat caccctcaac ccggccttgc gcagctcagc ttcaaccttg cgggcgtaat
     5221 ccaggtggcg gtcggcaatg ggaatgatgg ctgcctgggt aggtgaaagc caaacaggaa
     5281 aagcgccagc ataatgttcg atgagcacgc caaagaagcg ctccaggctg cccaacagcg
     5341 cgcggtggat catataaggg cggtgtggtt gaccatcttc gccgatatag tacaggtcaa
     5401 agcgctcggg ctcgttgaag tcgaactgga tggtcgagag ctgccactcg cgaccgatgg
     5461 cgtcttccac tttgagatcg atcttaggtc cgtagaaagc cccgccgccg cggtcaacgt
     5521 cgtagtccaa tcccgagcgg ttgagcgaat cttccagcgc tgcctcggca gcttcccatt
     5581 gcgccgggtc acctactgat ttttcggggc gagtgctgag ataggcatgg atatcggaaa
     5641 agccaaaact gcggaggata ttcaggctga agttaagcac gaagtcgatc tccgcgggca
     5701 tttggtctgg tcggcaaaag tggtgtgcat catcctgggt gaaaccacgc acgcgcatca
     5761 acccatgcag cacgccgctg cgttcatagc ggtacacggt gccttcctct gcgtaacgca
     5821 ggggcaggtc gcggtaagaa cgcagcgaat ttttgaaaat gtgcaggtgg aaggggcaat
     5881 tcatcggctt caagaaatac tgctgccctt caatatcgac cggtgagtac atattgtctt
     5941 tataaaagcc caggtgaccg ctggtttccc acagctgcgc cttgccgata tgcggggtgt
     6001 aaacgaaatc atacccattg gcttcatgct cttccgacca aaagttctca atcagttttc
     6061 gaatgcgccc gccctttggg tgccagagga tcaaccccgc gccaatctca tcgttcacac
     6121 tgtaaaggtc aagctcctgt ccaatcttgc ggtggtcacg cttcttggcc tcttccagcc
     6181 gccacaggta atccttcaat tcttctggct tgctccaggc ggtaccgtag atgcgctgca
     6241 gcataggacg tttctcatcg ccgcgccagt aagctcctgc aacattgagc agctttatcg
     6301 cggcggggtt gatctcgcgt gaattggtca cgtgcggtcc gcggcacaga tcggtaaagc
     6361 cgccctgcga gtagatggaa atctcgggtt tttcatctat cggattaccg tcatcatcca
     6421 tctcacccga ctcgaggcct tcaatcagtt ccatcttgaa aggctgatta cggaatagca
     6481 ctttggcctc atccgcgctc accactttgc gttcaaagtt gacccccgat tggatgatgc
     6541 cgcgcatacg cttttcgata ccctccaaat cttcgggggt aagcggtcgg ggcaggtcaa
     6601 aatcataata aaaaccatcc tcaatagcgg gaccgatggc caccttccct tcagggaatt
     6661 tttccagtac cgcctgcgcc atgatgtgcg cggttgagtg gcgaattctg tacagttgcg
     6721 agtcttcgta gtgttccttt tgtcttgcca ttctttcctc cattccaaga ttaaacaaaa
     6781 acgcccgtcc catactgggg cgagagttgc ctgcttcgcg gttccaccca gattaatgcg
     6841 gagaaaaacc gcatcatctc ttgtgaccat taacggagtc atccgtttcc cttaataggc
     6901 gagtgccgtt caggttcaag ctcgcgggtg gttttcgctg ttggtgttcc tgaaagaact
     6961 ttcagccagt ggttccttct ctctgagaga acggtcaaca ggtactcgtc ccggtcagcg
     7021 ccttgttgaa tgaaggtaat tatactccaa tttttttcaa acctaaggag aatttcgggc
     7081 ggaatagtcg ctgaaccttc caaccttgtt ttgctgccgg gtttcccaac ccacgcataa
     7141 aaagcaggag gccgcggggt tctcgccagc ctcctgctga aatacgccgg atgacacgaa
     7201 taaggttaaa acgcctggtc aggcttccaa accttccaaa ccttaccagc cagatctggg
     7261 cccggtgtta aggttggctt gccaggtacc caaccggatg gcgtgacttc tccggtcgct
     7321 accacatgct ggaaagcctg cacctggcgg atcaattcag acacattacg acccacttcg
     7381 ggggtcaaaa cttccattgc gcgaatattg aaatcaggat cgatcaagaa tctcccccgg
     7441 atgtccactc ctgcggtttc atcgtagacg ccgtaaatag tgcctacgcg cccgccagca
     7501 tctgagagca atgggaatgg cacgccgccc tccaccatct tggagagctc ctcttcctgc
     7561 caaattttat gcgaaaagcg gctgtcagtg ctgattgcca ggacttccac ccctaatgcc
     7621 tgtaattctt tgtatttgac ggcaactgcc gttaattctg tcggtcagac aaaggtgaaa
     7681 tcaccggggt agaaacaaat tacaatccat ttacccttgt aatcagaaag ggtaatattc
     7741 ttgaacccgc cctggtgaaa agcagttaac tcgaaatcag gcgcgggttt gccaactttt
     7801 gcttgaacca ttgacacctc cttttgaaat actggcgcag ctgagggggt ttctgatgca
     7861 gcggaaattg gacctttcac tggtttaaca caaccatctt tattttctgc cattcaacct
     7921 cctttttttt gccggcgtaa ttcttgcgtg taatttaatc atactaagat ctctcaaaaa
     7981 atcaatcgcc atccttcttc gacccaacgt gggggttaga atatcgagtt ccaatggaaa
     8041 acccagctta ccgaaccctc gccagcgggt ttggcagcca gttccacggg ttagccgatg
     8101 gcctttttga ccttttcctt ggtgtacggc aaatcacgca gccatacgcc aatggcgttg
     8161 tggatggcgc tggccatggc tggcgcaact ccatcaatgg aaatttccgc gactgatttc
     8221 acaccgtaag gaccggtggg ctcgtcagtt tgcacaaaga tcacatccat caccggcatc
     8281 tcaacagcac gcgggacgcg gtaggtcacg aggttgggat tgagcggttg accttcagca
     8341 gaatacagca tatcctcggt aagggcaaaa cccagcgcct gggccatgcc gccctctacc
     8401 tgcccggagg ctgttacagg gttgataaca cgaccgcaat ccaccaccat caccagccgt
     8461 tctgtatcca ctttgccggt ttcggtatcc accacgatct cgacaaattg cgcggcggtg
     8521 ggcggcgggc tcaccgggct aacgtgcgat tcggtggcca ttatttgatg ctgcgccatc
     8581 tgatgcaggc tgctgagacc aatctcctcg aagctcacct gtcgaccatc tgctgcaacc
     8641 acttttttat actccaggcg cagagtgttg aggtctttca cgcccatcat ttttcctgcg
     8701 tgttccagca gttgattctt aatattgacg gcagtcttgc gcaccgcttc tccggaaagg
     8761 taagcggtgc tgctggcata cgctccttta tcaaatgggg tgaagtcagt gtcagatgag
     8821 tagacgatga tgtcatccac atcggtgttg agcacttcag ccgcgatctg cgcgagtatg
     8881 gtatccgagc cagtgcccag gtcagtcgct cccataagga ggttaaacga accatcatcg
     8941 ttcatcttga gcgtggcagc ggccatatcc aggttggcca cgctgctgcc gtgcatcacc
     9001 accgccatgc cgatgccccg tcggtagcga cccgtttgat cgcggtaagc agcgcgtttg
     9061 gcatcaaaat cagtgaccct gcgcccaatt tccacgcatt gttccatggc gctggtaacc
     9121 agctcctggc gcacgcctgt gcgaccctcg ccaagttgct gcgaaaggta catctcttcg
     9181 cctactttga tccagttctt tttcttaaag tccaccacat ccagccccag cctatcggca
     9241 atttcgctca tgagcacttc tatgccatac tggcactgca tgctgccgta accgcggtaa
     9301 gccccagcca caacggtatt ggtataaaca acatcacaaa taaatcggga gttgggtgaa
     9361 ttgtacaacg tcagcccttt gaacccgccc accatggtga cggtgagccc atgagtggca
     9421 tacgcgccgg tatccccgat caggtacaga ctggctgcag tgactttatc ccctttcacg
     9481 cctatcttat agcggatggt ggtggcgtgg cgtgagcgcg agctggtgaa ctcctgctcg
     9541 cgggtgtact ccatgcgcac cggtcgtttg gtgatcatgg tcaaatgagc gaccaggtct
     9601 tcaatgagca cttcctgctt gttgccgaat ccgccgccga tgcgcggctt gatgatcctg
     9661 atcttcttca gcggcagacc gatgagcggc gcgataatgc ggcgcgtgtg gaaaggaact
     9721 tgcgtagagg tacggataac catgcgttcg tcttcatcaa aataactgat ggcaacatgc
     9781 ggttcaaggt gaacatgctg ctgtttctgg gtgtggtatt cgccttcgaa cacgtggtca
     9841 gattcagcaa aagctttatc tacatcacct acttcagctt cgatatgata aacaatgttg
     9901 cgctctcggt tataaatgcc ctccgtgtca ggttcgtcat ggattaccgg cgcacctggc
     9961 tgcatggcgg cttccatatc tacgaccgct ggcagtactt cgtactcgac atcaatcagt
    10021 ttgagagcct cgttcgcaat ttccaaagac tccgccgcca ccacagccac ccggtcgccg
    10081 acgaagcgca ccttgtcatc caggctgacc tggtcataag gtagcggttg tgggtagctc
    10141 tgcccgccgc tggcgtattt cacacgcggt acatcatggt gggtgagcac ggcgtgcaca
    10201 cccggcaagg cgcgcgcgcg gctggcatca atatgtttga tgcgcgcatg ggcgtgcgga
    10261 ctggtcagca tggcggcgta aagcataccc ggtaaatgaa tatcatcagt gaaaactggc
    10321 ttgccaagcg ccagcttgcg cgcatccact tttatctctg atttgcccac caccctggcg
    10381 gcgggcattg aagcgggtgt gtaaaccagt ggcgggaggg gatctcgatt gccatcagcg
    10441 cgaaagaact cagccggaat ttcaatctgc gccaggtcat ctggaagagg aaggctaata
    10501 tgattgaaag gcgccacttt ttcaccacgt ttgatggctg ccgcccgctg aaccgcgttg
    10561 acgccgcgaa catagcccgt gcagcggcag agcaccgggt ttagggcatt gcgaatctcc
    10621 ttctcggatg gttcaggctg atgatccagc aacgcttttg ccgaaagcat taatgccggt
    10681 gagcaaaagc cgcactggat ggcgccggtt tcgataaaag cctgctgaat gggatcgagc
    10741 tcgccattct tactcaagcc ttccagggtg atgatctggc tgccttcggc ttccatggct
    10801 ttaatggtgc aactgcgcac cgccttgccg ttcataatca cggtacaaga accgcattcc
    10861 ccgctgccac agccaaactt gacgctgaaa tacgaggcgg cgcgtagggc gtccagcaag
    10921 gtttcttccg gtgtcaccag taatgccttc ttttcaccat tgatggtcag ttgtacgctt
    10981 ttttgagcag gattcatggt tcctccaaac gcgggttaac gccgatcagg atttttattg
    11041 acaactctga atgcgttact attttattat agaatccaaa aaatgacctg ccgcggttgg
    11101 cgcggtttca gtgcttttcc attctggcaa tcaggattag ggaaaattcc accttggaaa
    11161 ctcaaaaaca agcagcacca aacccccatg tggttttgtc cctgcttcac tgggcgtata
    11221 aaccccgcga ctaccccccg ttcccgcggt ttccacaatg gaagttcgcg cctgtttcag
    11281 gtcagagcgg atggataaac tgaagtttgc gcatttaagt tggtggaata cggggtaact
    11341 caggcgtctt ttaccatgtt caccacctgc acgccccgca cttgcgggaa accagcgccc
    11401 tgactgatgg tgagcctggc agccgcttca tcgataatgc gcgaacccga gaggtaagtg
    11461 accccgtagt cgaacaacaa tggtaccagc ggggtggagg gaccgagaat catcaccggc
    11521 acgcctggac ggcacaagga gagcaacctc tctatggtgt ggttgatgaa agccgtgccg
    11581 gtaatggcaa caacatccgc gctcggcaga aattcctgcg cggcttcttc tgggaagtcg
    11641 tctccgtggg ggtccttctc aatgacccac aggttcgcgg cgaggtcacg cagtttcgta
    11701 ataaagggga aatgcccgat gacagccagg ttcttaccgg ggcattcgca tcttaacact
    11761 tctgaagcgt tcccttcttt cagcttggaa tcgtcaaggt caattaagga attgtaagcg
    11821 gcgatgccca ggctggcttc aagcaggttg tcagattcga tccagccggc caattcctga
    11881 gcgctctttt ctaacaggca gccaacgtcg cgcatgggcg cgtgcccatg cggtccgtag
    11941 ttgaccaggg ttgaccccag tccactgtgt ttactgcaaa ccaaagtcca gtgaacccct
    12001 accagaacac ttcgcagtgg taccggctct ttgggcagca acgccaataa atcagtatag
    12061 atgctcatct caactattca ggcgcaactc acacgccaaa aattcctgac agcaagaaaa
    12121 caaagatcga accgagaata atgccaatcg cggtgtagtg ttcatgccca tattcacgcg
    12181 cggtcgggat catttcatcc agggtgatga acaccatcac ccccccagcg aaagccagtg
    12241 ctgccggcac caatccctga aaattcacca aaaacaggct tgccagcagc gcgcctaccg
    12301 gttccgccag accagaaagg aaagccaccc ggaatgcgtc ccagcggcaa acgccgccct
    12361 tgcacaacgg gagcgcggtg gcaatgcctt cagggatgtt atgcaataaa atagccacgg
    12421 cgatgaataa accaaatttg gggttgtgaa ggtaaccagc gccaatcgag ataccttcag
    12481 gcaggttgtg aagcgtgatg cccaccgcca gcagcagacc agtgttgatg agtttatagt
    12541 cgtacattct gccctggcgg taaaggcggc caaacccgcg ccctccaggg cgcggaccca
    12601 tcctttggcg tggttcagcg caaggaggtt cacctcttac ttccttctca ccaaaacgta
    12661 tatgaggggc gaagtgatcc agcaggaaca tgaaaatcgc tcctgtggca aaaccaatgg
    12721 tggcagtgat gtaccccgcc tgcttccagg cctcggcaac gagctccaga aaagccaggg
    12781 aaatcatcac acccgcggtg atgcccatca gcagaccata agaccttctg cctggtcgtc
    12841 ggataatcgc gatcaacccg ccgagcccgg ttcccaaact ggccatcagg ctgaggagta
    12901 agatgttaag caagggggat tgtccgaaaa tcataaattc cttaataagg accattcatc
    12961 accatggatt aatgaagtgc cattgtaacc gaaatgcaca ttcgttccaa ggcgatccgt
    13021 tgatcatggt cactcatcct ggcaaatggc gcctgccgaa agcggatcaa agccctcagg
    13081 ccactgggtg gttttatcat acaacgctcc ctgcaaaaca gcgcaatcca ggtccgcgcc
    13141 ggtaaggtct gcgccgctca ggtttgcgcc gatgaggttc acgcccatca aacccgcgcc
    13201 acgcaggttg gccttgcgca gattcgcccc ctgtaaaatc gcgtgtttaa aggcagcttg
    13261 ctcgaggtca gcctcttcca ggttggcatc ccacaaaatg gaacgcttca ggttggtgct
    13321 cttgagatta gctcccgcaa gattcacatt tgccaggtct ttgccataca ggtcaatccc
    13381 ctccaggttt gccctggtgc catcgcactc gccggcgaga aacctggcgt gcgcctggat
    13441 gattctttgc aggtcttcgt aaatgatttt catcgagtgg gtcactccgt ttgcttatgc
    13501 ggcgtctctt atcctacagt aacttagtca taaacgtgaa tacatttaat gaagcagttc
    13561 aggatgatcc accaatttgc cttcgaggta taacttcacg gcttcgtcaa tatccttcaa
    13621 atctgtcacg atgggtgtga tttccaggcg gcgcatacta tcataagcgc ccatgcccat
    13681 acctccgcag agaacaactt tgcaatcaga aatcgtttct gccatgcttt tatgttttaa
    13741 gtgcgaacct tcatcaaagc cgtgccgatg accatgggtt tcttccgcgc gaccaccaaa
    13801 atgtccgtgc ccgggttttt cacgcatttc tctatgagtc accttgccat cttcaatcgt
    13861 caacaccagg aagtaaggag cccgcccaaa gtgctggctg atctttttac catcttccgt
    13921 aataaccgca attttcatgg ttttctcctt ttgtaatttt tcaagcgaaa catgccggct
    13981 gcctgcctct tcggctgcat ctgccggcgc cagaatcatc agcaaacggg cgagcgcggg
    14041 ttgattattt tcccaggcgt gggggagatg cgcctcaaaa attaggctat ctccggtttt
    14101 taatggataa acctcattgt caattgtgaa ctgcgcctgc ccctcgatac aatacacaaa
    14161 ttcatgcccg gtatgtacga tcatattatt tccgctgccc atgcctgcct ttaaggtcac
    14221 aaggtatggt tgtatggttt ttctggaaag gttttgcgcc aagtacacca gctgggtatg
    14281 gttgaatgct gtcgttggct gagggtgcgc gggtgtaaaa accacctgtt tttcgatggt
    14341 ttcctcaacg aaaaacgcag acaaagggac agatagggca gccgccagct gctgcagcgt
    14401 gcctacagag gtagaggttt ttgcgttttc cacaagtgag agcgtgttga tgttcaaccc
    14461 gctcagatcc gccagggaac gtaacgaaag ccctttgctc acacggagct tcctgagccg
    14521 ttgtcccacc atcatctcag tggtggtgtg ttcggttccc tggtcaggat ctgtcggaag
    14581 agtcaagttt ttactccacg gcgaacttaa tgggtgtttg attgaggatg ttatctgaga
    14641 tcgggcagcg ggcgtcaata tcgcggacaa attgctcttt ttcctgctgg gtcatgtctc
    14701 cgtcgatcgc aaccttcact ctgattcctt caaacccggc gcgcacgtcg ctgcgtttgc
    14761 ccatcagcac atctgtatcc aaatcgccat cgatttttac agagatcccg cataaaggaa
    14821 gttgctgctg tttggcaatg atctggccaa ttgtggcaac acaccctgcc aatgaaacga
    14881 acaggtactc caacggggtg gggccggcat ctcccccgcc accagcaggg ggttggtcta
    14941 caatcgaaac atgctgtctt gatttgactt caatcttgta tccttccaac aacactgctt
    15001 caacgcttac atttttattt ggcattctaa cctcacgtat aaaaagatgt atgaaaatta
    15061 tagtatacaa gccaggtttt catggttttt tttcccaaca taattataat tctcagggtt
    15121 ttttatgact tttatcatcc g
//
LOCUS       DAOTLW010000042        13946 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_4822_length_13946_cov_13.379238, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000042 DAOTLW010000000
VERSION     DAOTLW010000042.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13946)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13946)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13946
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4822_length_13946_cov_13.379238"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(132..1052)
                     /locus_tag="P5516_07040"
     CDS             complement(132..1052)
                     /locus_tag="P5516_07040"
                     /inference="COORDINATES: protein motif:HMM:NF012958.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HRT92225.1"
                     /translation="MWKNALRIDIQLKHIIAGVCALLLSACAALPPQETLPAAERSAP
                     VVSPVPTVTPTAALPTPARCDRGQGGEVERLTLHSELQGLEYAVSVYTPPCYGEEEGV
                     TYPVLYLLHGQNMDDTFWLSLGAAEIADELIAAGGRPFLMVMPQEVKNFDPVEESHFD
                     ASILNELIPWVEGHYRVCAERACRAIGGISRGGGWAMRLAARNFDTFGAVGAHSMGLM
                     QGDAWQIQKHLETRSLEEYPRIYLDRGEDDFLYEGIDYFEEILREKHIPHEFHINPGK
                     HDQAYWQAHVGEYLRWYAAGWEGRSSTVDK"
     gene            1217..2539
                     /locus_tag="P5516_07045"
     CDS             1217..2539
                     /locus_tag="P5516_07045"
                     /inference="COORDINATES: protein motif:HMM:NF024742.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92226.1"
                     /translation="MSEAIMDPRTDRKARRAWYLYDFGNSAYAAVVLLAVYSVYFSSV
                     VADHVRGPSLWNLAVGIAAIIVAVFSPVLGTIADFSRSKKKFLFLFTALSVVFTALLF
                     FVQKGDVFIGILFFILAEIGYRAAQVFYDALLVDVSTPETIGNVSGKGWAMGMLGGIA
                     CLVLVLVPIQLFPGNTLMVRVSFLITAVFYAVSSIPVLLRVRQVNEPETLPAGESTIQ
                     HAFKKLGSTFRDIKRYKEFVKYTVSFLIYNDGIMMLMDNAAIIAGILYGFKTSELIIL
                     IIILQLTGAGGAYLFGRVSNRQSSKQAILYSLAMLILVVALLFFTRNKVFFYIIGALA
                     GFALSGVQAVSRTMVSQLSPPTKTAEFYGFLSVAGRTSTFIGPWVFGSVYAAFTNHYL
                     NRGLSEQAAKSGGILWSIGSIVIFLVVGLLFLLIVRKVTPQDPLRFDE"
     gene            2577..3305
                     /locus_tag="P5516_07050"
     CDS             2577..3305
                     /locus_tag="P5516_07050"
                     /inference="COORDINATES: protein motif:HMM:NF013702.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT92227.1"
                     /translation="MKNHIPYPRRQFIRRILKSGIALAFGVLADVRVNGRENLPAGGP
                     LIVVGNHFHFLDPLMFIHLLPYPMEFVGGARMPNAPTVTHFLQRMFGVIPTARGSVSR
                     DTLYGAESILQQQGVLGIFPEGGSWATVLRPPRPGAAFLAWHTGARLLPIGIDGAVGF
                     FKHFGKRRAPVTLNIGKPFGPLTNGGSGRPSRAELDEIGHSIMRRIAELIPPERRGFY
                     SDDPAIREAARGTEIYPFASAAEI"
     gene            3422..4336
                     /locus_tag="P5516_07055"
     CDS             3422..4336
                     /locus_tag="P5516_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92228.1"
                     /translation="MKKLLLLSVPLVLLAGCNLPIGPGAKTPPPEPPLVIITEAPIPP
                     GFTQPAATEPPAEAPTPPPGTELNLGGIYMLIPPCLPVSASDLTVPAQVYDPEGGPQE
                     VYPQHRRLSFSGYPLSGTFFEPFMRVFPVEGFTSAYAAAPENYAAPIIAQMQTLLATR
                     PPDGDASLPFLVMAGAGQVFHAQAKYLDFANGSGVRYLTSYGQYYVPYNNHDLFYTFQ
                     GLTTDGKYWVSVIFPVNHPLLPPTYDSSLVPPGGIAIPSFTSPSYDEDMSAYYAAMKA
                     LMNSQPDNTFTPALDCLDAYITSLHVGD"
     gene            4593..6047
                     /locus_tag="P5516_07060"
     CDS             4593..6047
                     /locus_tag="P5516_07060"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRT92229.1"
                     /translation="MQLAYLNPLILFPVIGLIAFIYYILPRSTRWIFLLVASYAFYYS
                     VGSWTIIVLVAVTALNYFFGLGLGSGQFSKKRALLVCGIILNILFLFLFKYLSSLLAP
                     GSMLMTILASKTGRLLIPAGLSFYTLQNISYLVDVSKGIIKPERNPGIFALYLAFFPK
                     ILSGPIERGKKLLPQFHHPSALNWDDITAGMRLMLFGLFKKVVIADRLALFVNEVFNN
                     PGTYHSWTVMIALVFLSFQIYLDFSGYTDLALGIARVFGINLTQNFKRPYLSENIVEF
                     WNRWHLSFSTWLRDYIFYPLRRFFLKSGLKSTGFVSLGIPPVVTMALSGIWHGVGLTF
                     AVWGLYHAFFYTLVVWWRSTHTVNHQKPLVLKIFLVLVNFAVLTFSWLIFRSDNLQHA
                     FTLFNSLIVPGQMQYDLHEILLHTYVFDFQVSRVAILVVIAVEVFLEVKKDSFKFENA
                     PKWLRWSVYLLLILSLTTIGLYQGGDNPFVYFKF"
     gene            6103..7026
                     /locus_tag="P5516_07065"
     CDS             6103..7026
                     /locus_tag="P5516_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92230.1"
                     /translation="MNAVLLMNYRQNNLDSYVAAVFDKQALLSSTPSPKIILVGGSNV
                     AFGFDSRRLSEGTGLPVVNAGLQGGLGLHFMLNSIKPSINRGDIVLLSPEYHILFNRL
                     SWGEVLAQMLVIDPGSWKNISTFNEVWQLLKTFPTVHTEAIRNRFELEMNPKCQFCKS
                     DMSVYSREAFDPETGDITTNQLEGKPEEDFSMKLGYALPNFWIKKSIRSLNRFNEYVV
                     AKGATLFFVYPNTVTPTDAYTLDLLNRLDAYLQAQLDFPVLDTPSQAEFAKEYMFDTP
                     YHLNSKGRLLRSENIVIDLCKMDLPALHCQE"
     gene            complement(7340..10141)
                     /locus_tag="P5516_07070"
     CDS             complement(7340..10141)
                     /locus_tag="P5516_07070"
                     /inference="COORDINATES: protein motif:HMM:NF014442.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-6 DNA methylase"
                     /protein_id="HRT92231.1"
                     /translation="MPAPAIIHQLVARFTENREAYRSGKYNEARLRQEFLDPFFVALG
                     WDVYNQKGVAPQYADVEIEASLDIEGTSKSPDYTFKVGSERKFFVEAKKPAVNIRYDI
                     HPAFQLRRYAWNAHLPISILTDFEEFAVYNCMAKPGPKETAATARDLFYLYTDYIEKW
                     DEIAAIFSRDAVWKGALDRFAASSKGKKGTTEVDDEFLKDMNNWRVLLARNIALRNPR
                     VEDEQQLNYAVQITLDRIIFLRICEDRGIEPEEQLKTLSTQPGVYAGLLELFRHADLK
                     YNSGLFNFTHDKDDNTPPDTFTPSLTIDDRALKEIIQGLYYPCPYIFKEIPVEILGQV
                     YEQFLGQVIRLTAGHQAKVEEKPEVRKAGGVYYTPKYIVDYIVENTVGKLLEGKTPTE
                     AAALKIVDPACGSGSFLLGAFQYLIDWHERWYLANEPQKWTRGKAPALNEVTGGYRLT
                     TLKKKEILLNNIHGVDIDPQAVEVTKLSLLLKVLEEETGQLSLGLERALPDLGRNIQC
                     GNSLIGEDYFAGQLTVDPAERRRVNPFEWERAFPGVFAQGGFDAVIGNPPYGAFLATD
                     EEKYLVNHFETISSFRDVYICFIEKAVNLLKQSGTFSYIIPSGWTGGPGYIKLRNFLL
                     TKKIRKILLLPFDIFSEAYIDTLVLVASKAIPNEDHVVETYVYPKKIQISEIRGLAYQ
                     SIAQNTWLQTEDKKLILKNDSILLLNHLSQVGYSKIKNICDMKRGVLFDKKLLSPTPL
                     TNQYHPYFEGDVYRYSINIRLVNWVEFSDKLRERPKEFKWFQGDRILLRRLVNRRQRL
                     MGCFTDKTFITNKNLYSIIPYDIDTHFLLGVINSQLISYLYINQVTQAVKDDFPQVTI
                     TDFLNLPFPSVIDSQISNKIKALSRHLQNLNNMAPATPQEQEQLRREIEATDCQIDQL
                     VYQLYGLTDEEIKIVEEST"
     gene            10737..11159
                     /locus_tag="P5516_07075"
     CDS             10737..11159
                     /locus_tag="P5516_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92232.1"
                     /translation="MEEQRKRGAQPGNTNALKYGFYSRRLPSRELDGLEDTAVSSLKD
                     EIDVMRVFSRKVAELGADVDDLDEATTLLRVLSLATSSINRLVRTHTRIPDPDLDPAL
                     LLRTALLELEEEWPELKAFGDKYRNKPRVEPLPPEETL"
     gene            11345..>12024
                     /locus_tag="P5516_07080"
     CDS             11345..>12024
                     /locus_tag="P5516_07080"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HRT92233.1"
                     /translation="MTRPLRAALALTLALTLAFAVPLTGAPASSARAQYNGAYYTVQE
                     GDTLTEVAFYFRVSLNELIALNPMPDPNNLPPGKVLLIPGFEDLQGELKRVSVPAGEN
                     ARTLQRLYRLPPALFQRLNFVTAPDALITGHPFFYLEAAAREQTRLQLVSGLTPLELA
                     AYGAASSWLAGLYNDLPAPWRLLANDTLFLPGAQRGSLNALLPLVTDLALDQLAQGKT
                     SVFRASPSP"
     assembly_gap    12025..12124
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <12125..12912
                     /locus_tag="P5516_07085"
     CDS             <12125..12912
                     /locus_tag="P5516_07085"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRT92234.1"
                     /translation="LDQLAQGKTSVFRASPSPQTALSGELLGFPLRFFANASGGGFTA
                     LQGVPRLTEPGVTRFTIRAAAPDGRVFTLQQQAYLQPKNYGYDAPLEVMDSVVDPAIT
                     EPEFAFVTELTSPAPPEKLWQGRFAFPSSTPDFITSWYGRLRSYNGSDFTYFHSGMDF
                     GGGESSPILAPASGVVVYTGALDVRGNATIISHGWGVYTGYWHQSRIEVKVGDVVQTG
                     QTIGLMGATGRVTGPHLHFSVFVGSVEVDPEDWLNGQYAWVGG"
     gene            12914..13588
                     /locus_tag="P5516_07090"
     CDS             12914..13588
                     /locus_tag="P5516_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92235.1"
                     /translation="MLNPDCAYCGRHNPEGIERCQACGAPLAFAAAPETRVTIIEDAP
                     PAAPVQSAFPEALPSSGELQQGLKALGAVAGSLGVGSILLRLVAQGFAIAVSAFLVGL
                     SAGSSAAGLTRHALHLGCALAGGALLGIVISLVRKRSIWTLLAAPAGSFIAFLLNGLL
                     RLPSRQLPFAALLTLGGGLLFAVMGGRRARAKPLPCLKVLLPFIGAIGGVLFALLGFY
                     VMYRVY"
     gene            13609..>13946
                     /locus_tag="P5516_07095"
     CDS             13609..>13946
                     /locus_tag="P5516_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92236.1"
                     /translation="MSPTPYFKLEVYAPEENVDELLAVLAAAHAGEIGRYDHCASLTP
                     VQGTWRPLEGARPALGQPGELYSGSEVKIEVLCREEYLLEAVQALREVHPYQEPVINI
                     IPLANSRYGGT"
BASE COUNT         2653 a         4364 c         3588 g         3241 t
ORIGIN      
        1 gtgggcggcg ggcgcgccgc ataaagggtg atgccttacc gacctgtctg gttaattata
       61 tcaacgggct cccctcggcg cccacaaccc tgccccggtt ttagccggcg cgggcggctg
      121 cgggcggtct ttcacttgtc aaccgtggaa cttctcccct cccatcccgc ggcgtaccag
      181 cgcaggtact ctcccacgtg cgcctgccag taagcctggt cgtgcttgcc ggggttgata
      241 tggaactcgt gcggaatgtg tttctcacgc aggatctctt cgaagtaatc gatgccctca
      301 tagaggaaat catcctcgcc gcgatccagg tagatgcgcg gatactcctc cagcgaacgc
      361 gtctccaggt gtttttggat ctgccaggcg tcaccctgca tcagccccat ggaatgggcg
      421 ccgaccgcgc cgaaggtatc aaaattgcgc gccgccaggc gcatggccca cccgcccccg
      481 cggctgatgc cgccgatggc gcggcatgcc cgttctgcgc acaccctgta atgcccctcc
      541 acccagggga tgagctcgtt gaggatggag gcgtcaaaat ggctttcttc aaccgggtca
      601 aaattcttca cttcctgcgg catcaccatc aggaaaggtc tgccgccagc ggcgatcagc
      661 tcgtcggcga tctccgccgc gcccaggctc agccagaagg tatcgtccat gttctgcccg
      721 tgcagcagat agagcaccgg gtaggtaacc ccctcttctt cgccgtaaca gggcggtgtg
      781 tacacgctga cggcgtactc cagcccctgc agttctgaat ggagggtgag gcgctccacc
      841 tccccaccct gcccgcggtc gcagcgggcg ggcgtgggca gcgcggcagt gggggtaacc
      901 gtgggcacgg gcgaaaccac cggcgcgctc cgctcagccg ctggaagggt ctcctgcggc
      961 ggcagcgccg cgcaggcgct caacagcagg gcgcacaccc cggcgatgat gtgtttgagt
     1021 tgtatatcta tgcgcaacgc gttcttccac atggatttca ttctccctgg ctcaatttta
     1081 ctcaaccgcc ccccgctgcg gaatacccat tataatagcc gaatcacgca accaatgaaa
     1141 tcgctttggg ttataaatga tgttaacgat cgtttgtacc tgtacctttc tcaatctttc
     1201 tctgtgggag taaatcatgt ctgaagccat tatggatcca aggaccgacc gaaaggcgcg
     1261 ccgcgcctgg tacctgtatg atttcggtaa ttccgcttac gccgccgtgg tcttgctggc
     1321 ggtctattcc gtgtacttct ccagcgtggt cgctgaccac gtgcgcggcc cctcgctgtg
     1381 gaacctggcg gtgggcatcg ccgccatcat cgtggcggtc ttctcgccgg tgctcggcac
     1441 gatcgccgat ttctcgcgct cgaaaaagaa attcctcttc ctctttaccg ccctctcggt
     1501 ggtgttcact gcgctgcttt tcttcgtgca aaaaggcgat gtgttcatcg gtatcctctt
     1561 ttttatcctg gctgagatcg gttaccgcgc cgcgcaggtc ttctacgacg cgctgctggt
     1621 ggatgtttcc acgcctgaaa ccatcggcaa cgtttcgggc aaaggctggg cgatgggcat
     1681 gttgggcggt attgcctgcc tggtgctggt gctcgttccc atccagctct tccccggcaa
     1741 caccctgatg gtgcgcgtct ccttcctcat caccgccgtt ttctacgccg tctcctccat
     1801 ccctgtgctg ctgcgcgtca gacaggtcaa cgaacctgaa accctccccg cgggtgaatc
     1861 caccatccag cacgccttca aaaagctcgg ctccaccttc cgcgatatca aacgctataa
     1921 agagttcgtc aagtacaccg tctccttcct catttacaac gacggtatca tgatgttgat
     1981 ggacaacgcc gccatcatcg ccggcatcct ctacgggttc aagacctccg agctgatcat
     2041 cctcatcatc atcctgcagt tgacgggcgc gggcggagcc tacctctttg ggcgcgtttc
     2101 caaccgccag agcagcaagc aggccatcct ctattcgctg gcaatgctca tcctggtggt
     2161 ggcgctgctc ttcttcacgc gtaacaaagt gttcttctat atcatcggcg cgctggccgg
     2221 ctttgccctt tccggcgtgc aggcggtgag ccgcaccatg gtcagccagc tttctccccc
     2281 caccaagacg gccgagttct acggcttcct ttcggtcgcc gggcgcacct ccaccttcat
     2341 tggtccctgg gtgttcggtt ctgtctacgc cgctttcacc aaccattacc ttaaccgcgg
     2401 cctcagcgag caggcagcca agagcggcgg catcctctgg tccatcggtt ccatcgtgat
     2461 cttcctcgtt gtggggctgc tcttcttgtt gatcgtgcgc aaggtcaccc cccaggaccc
     2521 gctgcgtttt gatgagtagc gccaatgctt tcatttgaga ggacaagcat ccacgcatga
     2581 aaaatcacat tccctatccg cgccggcagt tcatccggcg catccttaaa tcgggcatcg
     2641 cgctggcctt cggcgtgctg gcggacgtgc gggtgaacgg tcgcgagaac ctgcccgccg
     2701 gcggaccgtt gatcgtggtg ggcaaccact ttcatttcct tgacccgctc atgttcatcc
     2761 acctgctgcc ctaccccatg gagtttgtcg gcggcgcgcg catgcccaac gcgccgacgg
     2821 tcacgcattt tctccagcgc atgttcgggg tcatccccac ggcgcgcggc agcgtctcgc
     2881 gcgatacgct gtacggggct gaatccatcc tccagcagca gggcgtgctc ggcatcttcc
     2941 ccgaaggcgg cagttgggcc accgtgctgc gcccgcctcg cccgggcgcg gccttcctcg
     3001 cctggcacac cggcgcgcgg ctcctgccca tcggtattga cggcgcggtg ggttttttca
     3061 agcacttcgg caaacgccgc gcccccgtga ccctcaatat tggcaagcct ttcggaccgc
     3121 tcaccaacgg cggcagcggg cgcccctcgc gcgctgaact ggacgagata gggcactcca
     3181 tcatgcgccg catcgctgag ctcatccccc ccgagcgccg cgggttctac tcagacgacc
     3241 ccgccatccg cgaagccgcg cgcggtaccg aaatttaccc tttcgccagc gccgccgaaa
     3301 tctgagccac ccctccccgc cgcgaagatc gttcaacccg gttttcacca ccgcgtttga
     3361 gcgaaatttg cgctatgatt aggttgtcaa cctgttgcgc ctatcatgaa ggagggaacc
     3421 catgaaaaaa ttacttctcc tgtctgtgcc attggtttta ttggcaggtt gcaacctgcc
     3481 catcgggccg ggcgcgaaaa cgccgccccc tgaaccgccg ctggtcatca tcaccgaagc
     3541 ccccattccg ccgggcttca cccagccagc cgccaccgaa ccgccggccg aagcgcccac
     3601 cccgcccccc ggcaccgagc tcaacctcgg cggcatctac atgctcatcc cgccctgcct
     3661 gcctgtctcc gccagcgacc tcaccgtgcc cgcccaggta tacgaccccg aaggtgggcc
     3721 gcaggaggtg tacccgcagc accgccgttt gagcttcagc ggctacccgc tcagcggcac
     3781 cttctttgag ccgttcatgc gcgtcttccc ggtggagggg tttacctccg cctacgccgc
     3841 cgcgcctgaa aactacgccg cccccatcat cgcccaaatg caaaccctgc tcgccacccg
     3901 cccgccggat ggcgacgcct ccctgccctt cctggtaatg gccggcgccg gtcaggtctt
     3961 ccacgcccag gcgaagtacc tcgattttgc caacggctcg ggggtgcgct acctcacctc
     4021 ttacgggcag tattacgtgc cctacaacaa ccacgacctc ttctatacct tccaggggct
     4081 caccaccgac ggcaagtact gggtgtcggt catcttcccg gtcaaccacc ccctgctgcc
     4141 gcccacgtat gattccagcc tggtgccccc cggcggcatc gccatcccct cgttcacctc
     4201 gccttcgtac gacgaggaca tgagcgccta ctacgccgcc atgaaggcgc tgatgaacag
     4261 ccagcccgat aacaccttca cccccgcgct ggactgcctg gacgcctata tcacctccct
     4321 ccacgtcggg gattgagcgc ggcgcgtatt tttgaggaat gcgcccctgg cgtgtacttg
     4381 gtacgcccag catgaaaact gctgggcgtc tttacctgcg ccagggtgcg ccagcctgcg
     4441 cggatgaggc gcttcccccg cgttccaggt tgctcgtgca gcatccatcg tcatctcctt
     4501 aaataaaacc actggtgctt cataatagga tggttaattc gctggaatac aggtaaaatg
     4561 gttcattgtt aaattcaatg taaactgtat atatgcagct ggcttatctc aatcccctta
     4621 ttctttttcc tgtaatcggc ctgatagcct tcatatacta tatccttcca cgcagtacgc
     4681 gctggatatt tctgctcgtc gccagttacg ctttctatta cagcgtggga tcgtggacta
     4741 tcatcgtctt ggtcgctgtc accgcgctca attacttttt tggtcttggc ctgggatctg
     4801 ggcaattttc aaagaaacgg gccttgctgg tatgtggaat tatcctcaat attctgttcc
     4861 tgtttctgtt caagtatcta tccagcctcc tggctccagg ttcaatgctg atgactatcc
     4921 tggcttcaaa gaccgggagg cttctgatac cagccggctt atctttttac acgctgcaaa
     4981 acatcagtta cctggtggat gtatctaaag gaataatcaa accagagcgc aacccaggga
     5041 tttttgccct ctatcttgct ttttttccga aaattctgtc ggggccaatc gagcgcggaa
     5101 agaaattact ccctcaattt caccatccat ccgctttgaa ttgggatgat attaccgccg
     5161 gtatgcgctt gatgttgttt ggcttgttta aaaaagtggt cattgccgac aggctggccc
     5221 tttttgtgaa cgaagtcttt aacaaccccg ggacttatca cagctggacc gttatgattg
     5281 cgctggtttt tctgagtttt cagatttacc tggatttctc cggttatacc gatctcgcgc
     5341 tgggtatcgc cagggtattt ggcatcaacc tgacccaaaa tttcaaacgc ccttatctct
     5401 ctgaaaacat cgtggagttt tggaatcgct ggcacctctc gttttctaca tggctgagag
     5461 attatatctt ttaccccctg cgaagatttt tcttaaaatc gggtctcaag agtaccgggt
     5521 ttgtgtcctt gggtattcct ccggtggtca ccatggcttt gagcgggata tggcacggcg
     5581 ttggattaac ctttgccgtc tggggtttgt accacgcctt cttctatacg ttggtcgtgt
     5641 ggtggcgaag cacccatacg gtaaatcacc aaaagcctct ggttttaaag atatttctcg
     5701 tattggtgaa ctttgccgtg ctgactttta gttggctgat ctttcgttcg gacaacttgc
     5761 agcacgcgtt tacgcttttc aatagcctaa tcgtacccgg gcagatgcaa tatgacctgc
     5821 atgaaatttt acttcacacc tatgtgttcg attttcaggt ttcccgtgtt gcgatcctgg
     5881 tggtcattgc agtagaagta tttctcgagg tcaaaaagga ttcgttcaag tttgaaaacg
     5941 ctccaaaatg gcttcgctgg agtgtttatc ttcttttgat tctttcgtta accacaatcg
     6001 gtttgtacca gggaggagac aacccctttg tctacttcaa attctaggcg tctcctggtt
     6061 ttcaccatta accttctcag cattttcctg ataacgctgg tagtgaacgc tgtccttttg
     6121 atgaattacc gccagaacaa cctcgatagt tacgtggcag ccgtttttga taaacaagcc
     6181 ttgctttctt ccaccccatc gcccaaaatc atcctggtgg gaggttctaa cgtagccttc
     6241 ggcttcgaca gccggcgcct gagtgaaggc acaggtctgc cggtggtaaa tgccggctta
     6301 cagggcgggc tcgggttgca ttttatgctc aacagtataa aaccctctat taatcgcggg
     6361 gatattgtgc tcttatcccc cgagtaccac atccttttta accgccttag ttggggtgag
     6421 gtgctggcgc aaatgctggt gatcgacccg ggaagctgga aaaatatttc aacattcaat
     6481 gaagtatggc aattgcttaa aacatttcca accgttcata cagaagccat ccgcaatagg
     6541 tttgaacttg aaatgaaccc caaatgtcaa ttctgtaaaa gtgatatgag tgtctattca
     6601 cgagaagcct tcgaccctga aacaggtgat atcaccacca accagttgga aggcaaacct
     6661 gaagaggatt tctccatgaa actgggttat gctttgccca atttctggat taagaaatcc
     6721 attcgctcac tgaacaggtt caatgaatat gtggtcgcga aaggcgcgac gctcttcttt
     6781 gtctacccca atacggtcac accaaccgat gcatacactc ttgacctctt aaaccgcttg
     6841 gatgcatatc ttcaagcgca acttgatttc cccgtccttg ataccccctc ccaggctgag
     6901 ttcgcgaaag aatacatgtt cgatacgccg taccatctga attcaaaagg tcgtttgctc
     6961 aggtcagaga atatcgttat tgatttgtgc aaaatggacc ttccagcctt gcactgtcag
     7021 gaataacgtt tcgcttctcc actgaaggac aatatccaaa gaatggccct gatagggcca
     7081 tatccaatgc gcaccttttt atgagtgctc gtctctgcgg cgttcgctga atacccgcgg
     7141 cgcgctcagg ggcgtggggg ttgggcggcg tgaaacgccg cgccgggctg cctggacgcc
     7201 tatatcacct ccctccacgt cggggattga gatacgttat gttggggcgc gccccaatat
     7261 tttcccccgg ttgttcatcc tccacagccc aataaagggg attggtttgg atgtagtaga
     7321 ggcgtatggc ctaacgctcc tacgtggatt cttccacaat tttgatttcc tcgtcggtga
     7381 ggccgtagag ctggtagacc agctgatcga tctggcagtc ggtggcttct atctcgcggc
     7441 gtagctgctc ctgctcctgg ggggtggcgg gagccatatt atttagattt tgtaaatgtc
     7501 ttgatagtgc cttaatttta tttgagattt gactatctat gactgaaggg aaaggtaagt
     7561 taagaaaatc cgtaatggta acttgaggaa aatcatcttt cactgcttgt gtaacttgat
     7621 tgatatacaa ataggatatt aattgactgt tgattacgcc taatagaaaa tgagtatcaa
     7681 tatcatatgg aataattgaa tatagattct tgtttgttat gaaggtttta tctgtaaaac
     7741 aacccattaa tctttggcgc ctattaacta accttcttaa taatattcgg tcaccttgaa
     7801 accatttaaa ctcttttggt ctctccctta acttatcact aaattcaacc cagttaacta
     7861 gccgaatgtt tatcgagtat cggtaaacgt caccttcgaa atatgggtgg tattggttcg
     7921 tcaagggtgt tggacttaat aatttcttgt caaatagcac tcctcttttc atgtcacaga
     7981 tatttttaat tttcgaataa cctacttgtg atagatgatt aagcaacaaa atagaatcat
     8041 ttttgagtat caactttttg tcttctgtct gaagccacgt attttgagca atagattgat
     8101 aagcgagccc tcgaatttct gaaatttgta ttttttttgg atacacatat gtctcgacaa
     8161 cgtggtcctc gttaggaatt gctttagaag caactagaac caaagtatca atatatgctt
     8221 ctgaaaatat atcaaatggt aatagaagta tcttgcgtat tttttttgtt aataaaaaat
     8281 tacgcaattt aatgtatccg ggtccaccag tccaacctga aggaataatg taagaaaatg
     8341 ttcctgattg ttttaataga tttacagctt tttcaataaa acagatataa acatccctaa
     8401 aactactaat cgtttcaaaa tgatttacca gatatttttc ttcatcagtg gctaaaaatg
     8461 ctccatatgg cggattgccg atgaccgcgt caaaccctcc ctgcgcgaac acccccggga
     8521 aagcgcgctc ccactcgaag ggattaaccc gccggcgctc ggcagggtct accgtcaact
     8581 gcccggcgaa gtagtcctcc cctatcagcg agttgccgca ctggatgttc ctgcccaaat
     8641 ccggtaaggc gcgctccagc cccagcgaga gctgcccggt ctcctcctcc agcaccttga
     8701 gcagcaggga aagcttggtc acctccaccg cctgcgggtc gatatccaca ccgtgaatat
     8761 tgttgagcag tatctccttc ttcttcaacg tggtcaggcg gtagccaccg gtcacctcgt
     8821 tgagcgctgg ggctttgccc ctcgtccact tctgcggttc gttggccagg taccaccgct
     8881 cgtgccagtc gatgaggtac tggaaggccc ccagcaggaa ggagccggag ccgcaggccg
     8941 ggtcaacaat tttgagggcg gcagcttcgg tgggtgtttt gccttccagc agtttgccca
     9001 ccgtgttttc tacgatgtaa tccacgatgt acttgggcgt gtaatacact ccgcccgctt
     9061 tgcgcacctc gggcttttct tccaccttcg cctggtgccc cgcggtcaaa cggatgacct
     9121 gccccaaaaa ctgctcgtat acctgcccta gtatctccac ggggatctct ttgaagatgt
     9181 acgggcaggg ataatacagc ccttgaatga tctccttgag cgcacggtca tcgatggtga
     9241 gcgagggggt gaaggtatcg ggcggggtgt tgtcgtcctt atcgtgggtg aagttgaaca
     9301 gccccgaatt gtacttaagg tcagcgtgcc tgaatagttc cagcaagccc gcgtacacgc
     9361 ctggctgggt ggagagggtt ttgagctgct cttcaggctc gataccgcgg tcttcgcaga
     9421 tgcgcaggaa gataatacgg tcaagggtta tctgcacagc gtagttgagc tgctgctcat
     9481 cctctacgcg cgggttgcgc agggcgatat tgcgcgccag caggacgcgc cagttattca
     9541 tatccttgag aaattcatca tcgacttcgg tggtgccctt cttgcctttc gaggaggcgg
     9601 cgaagcggtc aagcgcgcct ttccagaccg cgtcgcgcga aaagatcgcg gcgatttcgt
     9661 cccatttctc gatgtaatcg gtataaagat aaaagagatc gcgcgcggtg gcggctgttt
     9721 ccttggggcc aggttttgcc atgcagttat agaccgcgaa ctcctcgaaa tcggtgagga
     9781 tggaaatggg caggtgggca ttccaggcgt aacggcgtaa ttggaaggcg gggtgaatgt
     9841 cataccggat gttgacagcg ggtttcttgg cctctacaaa gaacttgcgc tccgatccta
     9901 ctttaaaggt gtaatcaggg gatttggatg tgccttctat atcgagagaa gcttcaattt
     9961 ccacatcggc atattgcggc gcgacgcctt tttggttgta cacgtcccaa ccgagggcga
    10021 cgaagaaagg gtctaggaat tcctggcgca ggcgcgcttc gttgtacttg ccggaacggt
    10081 aagcttcgcg gttctcagta aatctggcaa cgagctggtg gatgatcgct ggcgcgggca
    10141 tgggcgctta cctcctgagt gaatggtggc aaattgattg tatatcagaa tagggtttta
    10201 gcaccccccg ggctccgggt gtatcagcga gcaaaaacgc ctgaaagggg cgtcttcagg
    10261 ctgtgccctc tcgtttcctt tccccttctc ttccattgac caccaaaaac acttgacagc
    10321 catgttataa tgattaatag aactgttgtt ctattccacc cccacccctt ttacccagga
    10381 gaaaaatgcc ccgcccgtcc accccaccac cccctccgcc gcccccgccc ggcggctcca
    10441 ctctcgtctt cgatttccgc gacctgcccc ccagccccga ggagctagcc gcccttcaag
    10501 cgcagaaaaa gcaccgccgc ctcgcctccc tcaaaacccg ccgccggctc agcgccaagg
    10561 ccctccccgc ttctccgctc ccgggcgcgc cgctcgaccc cgccccggct gatgccatac
    10621 cccaaaccgc tcccccagcc ccattcgccc ccgcggccgc cccgctgccg gaatgggccg
    10681 aagacctgcg cccgcccgct cgtgttcagc caggcccgtt ccccgctcca gccgcgatgg
    10741 aggagcagcg gaaacggggc gcgcagcccg gcaataccaa cgccctcaag tacggcttct
    10801 attcccgccg cctcccttcg cgtgaactgg acggtctcga agacaccgct gtttcttccc
    10861 tcaaagacga gatcgatgtg atgcgcgtct tttcccgtaa ggtcgccgag ctcggcgcgg
    10921 atgtggacga cctcgacgaa gccaccactc tcctccgcgt gctctccctc gccacctcgt
    10981 ccatcaaccg cctcgtccgc acccacacgc gcatccccga ccccgatctc gaccccgccc
    11041 tgctcctgcg caccgccctc ctcgaactcg aagaagaatg gcccgagctc aaggcttttg
    11101 gcgataaata ccgcaacaaa ccccgggtcg agcccctccc gccggaagag accttgtaaa
    11161 ccggcagaac agggttcgct tgcttctcag cccgccccgg ccgatgccat ccgcgccccc
    11221 cgcctgaagc gaaggcgcct cgccactggg aggattcgtt ttgattcgtt tttttaaaac
    11281 gaatgcccgc ggggattctt ttgaatttgg aaaaaccggc ttttctccat tacaatagac
    11341 cctgatgacc cgacccctgc gcgccgccct ggcgctgacc ctcgccctca ccctggcttt
    11401 cgccgtgccg ctcactggcg cgcccgcctc ctcggcgcgc gcccagtaca acggcgccta
    11461 ttacaccgtc caggagggcg acaccctcac cgaggtggcc ttctacttcc gcgtttcgct
    11521 caacgagctc atcgccctca accccatgcc agaccccaac aacctgccgc ccggcaaggt
    11581 gctgctcatc cccggctttg aagacctgca gggtgagctc aagcgcgtca gcgtgcccgc
    11641 cggcgagaac gcgcgcacgc tccagcgcct ttaccgcctg ccgcccgcgc tctttcagcg
    11701 gctcaacttc gtcaccgcgc ccgatgcgct gatcaccggg cacccgttct tctacctcga
    11761 agcagcggct cgcgagcaga cccgcctgca gctggtgagc gggctcaccc cgctggaact
    11821 ggcggcttac ggtgctgcca gctcctggct ggccgggctg tacaatgacc tgcccgcccc
    11881 ctggcgcctg ctcgccaacg acaccctctt tttgcccggc gcgcagcgcg gcagcctcaa
    11941 tgccctgctg ccgctggtca ccgatctcgc cctcgaccag ctcgcccagg gcaaaacctc
    12001 ggtcttccgc gccagcccct cgccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12061 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    12121 nnnnccctcg accagctcgc ccagggcaaa acctcggtct tccgcgccag cccctcgccg
    12181 cagacggcgc tttcaggcga actgctgggc ttcccgctgc gcttcttcgc caacgcctcg
    12241 ggcggcggct tcaccgcgct gcagggcgtg ccgcgcctca cagagccggg ggtcacccgt
    12301 ttcaccattc gcgccgcggc ccctgatggg cgcgtattca ccctgcagca gcaggcttac
    12361 ctgcagccca aaaactacgg ctacgacgcc ccgctggagg tgatggacag cgtggtcgac
    12421 cccgccatca ccgagcccga gttcgccttc gtcaccgagc tcacctctcc cgccccgccc
    12481 gaaaagctgt ggcaggggcg cttcgccttc ccctcgagca cacccgactt catcacctcc
    12541 tggtatggcc gactgcgctc ctacaacggc agcgatttca cctatttcca ctccggcatg
    12601 gatttcggcg ggggagaatc ctcgcccatc ctggcccccg cgtcgggcgt ggtggtgtac
    12661 accggcgcgc tggatgtgcg cgggaacgcc accatcatct cgcatggctg gggcgtgtac
    12721 acgggctact ggcaccagtc gcgcatcgaa gtaaaggtcg gcgacgtagt gcaaaccggg
    12781 caaaccatcg gcctgatggg cgcgaccggg cgcgtcaccg gcccgcacct gcacttctct
    12841 gtcttcgtcg gcagcgtgga ggtggacccc gaagattggc tcaatgggca atacgcctgg
    12901 gtgggagggt agcatgctca accccgattg cgcgtattgc gggcggcaca accccgaagg
    12961 catcgagcgc tgccaggctt gcggcgcgcc gctggctttc gcggcggccc ccgaaacgcg
    13021 cgtcaccatc atcgaagacg cgccccccgc cgctcccgtg cagtccgcct tccccgaggc
    13081 attgcccagc agcggtgaac tgcagcaggg gctcaaggcg ctgggcgcgg tggccggctc
    13141 gctgggcgtg ggcagcatcc tcctgcgcct ggtcgcgcag gggttcgcca tcgccgtctc
    13201 cgcctttctc gtcggtctca gcgcgggctc cagcgccgcg gggttaaccc gccacgcgct
    13261 tcacctggga tgcgcgctgg caggcggcgc gctgctgggt atcgtcatca gcctggtgcg
    13321 caaacgctcc atctggacgc tcctcgccgc gcccgcgggc agtttcatcg ccttcctgct
    13381 caacggcctg ctgcgcctgc cttcccgcca gctgcccttc gccgcccttc tcaccctggg
    13441 cgggggtctg ctcttcgccg tcatgggcgg caggcgcgcg cgcgccaagc cgctgccctg
    13501 tctcaaggtg ctgctgcctt tcatcggcgc catcggcggt gtgctcttcg ccctgctggg
    13561 cttttacgtg atgtaccgcg tttattaagg ttgtgcaagg agccgctctt gtcccccacc
    13621 ccctatttca aactcgaagt ttacgccccc gaagaaaacg tggatgagct tctcgccgtc
    13681 ctcgccgccg cgcacgccgg cgaaattggc cgctacgacc actgcgcctc cctcaccccg
    13741 gtgcagggca cctggcgacc gctggaaggc gcccggcccg cgcttggtca acccggcgag
    13801 ctctacagcg gcagcgaggt caagatcgag gtgctctgcc gcgaagagta cctgctggaa
    13861 gcggtgcagg cgctgcgcga ggtgcaccca taccaggagc cggtcatcaa catcatcccg
    13921 ctggccaaca gccgctacgg cggcac
//
LOCUS       DAOTLW010000043        13915 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_4833_length_13915_cov_14.279293, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000043 DAOTLW010000000
VERSION     DAOTLW010000043.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13915)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13915)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13915
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_4833_length_13915_cov_14.279293"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1129
                     /locus_tag="P5516_07100"
     CDS             <1..1129
                     /locus_tag="P5516_07100"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HRT92237.1"
                     /translation="RIMNEQGYRYIYDADILLSIQRGIYPQAIKAPDIFEEVPLPYLP
                     FFLVTFQLFSLIDLPFSYLTWSVLNIIVFSLYLKFFYKEIHAETIKSQLLLIMMLSLP
                     VFLNLYYGQLNIWLGICTGEFIRAIFYKKPVKAGIWLGGLLLKPQLLILLLPFLLLKR
                     ELKVIAGFLISSIGIGLISWILIGREGFLDLFGLIVSSSQGGGASNPQAMMNFRMVGT
                     FVEYFVNETFGKILVAVCSAITALVFFLIFSRQQKLDKSSIGFTSLGVMAATCAVTWH
                     AHIHMSIVLIPPLLFLIHDHAVERTVKYWFILFPTILLIVYLVAGLVGAGLVPRTVIP
                     FFDLFLGMVGLVFNLIFLRLASVGAGRINQNILDNSQHLSA"
     gene            complement(1140..1361)
                     /locus_tag="P5516_07105"
     CDS             complement(1140..1361)
                     /locus_tag="P5516_07105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92238.1"
                     /translation="MKPILLLDETLKVEVFYACEDHDLEDNICLKLTEDCAESERLLR
                     NEESHLYLTAPQARAFAEALLAAVEKSGG"
     gene            1503..1709
                     /locus_tag="P5516_07110"
     CDS             1503..1709
                     /locus_tag="P5516_07110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92239.1"
                     /translation="MPVIFFKLNYLDPGSGSLLVQLLAAGLIGGIGILLKMFWGRIKA
                     FFKGEKYAPPQPAAEEEPRETEGE"
     gene            1706..3094
                     /locus_tag="P5516_07115"
     CDS             1706..3094
                     /locus_tag="P5516_07115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92240.1"
                     /translation="MKAQAERSGASFRDPSGFLFTRDGVLYRQVNKAYQQAYEKLMSG
                     GVYHSLTLQGMLIRHEEVDAPAADPALAYRVIRPEEVPFISYPYEWSFSQLKDAALLT
                     LACARAALENGMVLKDASAYNIQFVNGKPLLIDTLSFDLYQEGAPWDAYRQFCQHFLA
                     PLALASMVDIRLTQLLRVYLDGIPLDLASVLLPGKTRLGFSGLAVHLHLHARAQRQYS
                     DRQAAQAAAVKLSKTALLHILNGLEKTILPLTWQPRGTEWGAYYSLTNYTDESLQAKG
                     ALVGSFAEQVKPRSAWDLGANNGLFSRELSRRGIETVASDIDPAAVEQNYLAVKAGNE
                     PHLLPLLIDLTNPSPALGWAHRERSSMLARGPVDLVMALALVHHLAISNNVPLPLVAE
                     FMANAGRWAIVEFVPKSDSQVKRLLSTRKDIFDQYNQEGFEEAFALYFNTERKAPIPG
                     SQRTLYLFKRKN"
     gene            complement(3197..4279)
                     /locus_tag="P5516_07120"
     CDS             complement(3197..4279)
                     /locus_tag="P5516_07120"
                     /inference="COORDINATES: protein motif:HMM:NF017864.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HRT92241.1"
                     /translation="MPNPHYVPLVETTRGGIVESIHYGSIAVCDTQGRLLARAGDAEA
                     LVFLRSSSKPFQALPLVEGGGMERFGLNDRELALMCSSHSGTDEHVAVLTALQRKLGV
                     SAAELLCGTHTPSDPLTAERMFLRGEENSPLRHNCSGKHTGMLAQALLYGDPRQDYID
                     PGHPVQQRILRVFSEMTGVPVPEIILGTDGCSAPVFAVPLRAAAYAFARLADPSALPE
                     PRRSALRRIFSAMTSHPDMVAGPDTFDTRLMTAGRGRVLTKGGAEGYQALAVLPAGSS
                     GAMGITLKISDGDLAQADRGQRANAIAPRGGGRARSTAALEALRQLGVLDSAQQEELS
                     DFAPRALTNWRNIPIGEIRPVFNLKN"
     assembly_gap    4519..4618
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4619..5189
                     /locus_tag="P5516_07125"
     CDS             <4619..5189
                     /locus_tag="P5516_07125"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HRT92242.1"
                     /translation="VNMIEERPLHVLGISGSLRVDSYNTALLRAAAELLPPGMTLEIT
                     DLAGIPLFNQDEENPFPEEVARLRARIAAADALLFATPEYNSSISGVLKNALDWASRA
                     PDMPLAGKPAALMGASTGVFGTARAQLHLRQVLAHLGALVLTKPEVMVMRAADAFDEQ
                     GRLVNEVTRGFLGQLLVELAQWTRLVSAR"
     gene            complement(5295..5540)
                     /locus_tag="P5516_07130"
     CDS             complement(5295..5540)
                     /locus_tag="P5516_07130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92243.1"
                     /translation="MNVHVQLYSPLKEFCGESRLELTLPEGAAATLMLDTLAARYPVI
                     RPFLRNAQVIIQNEMVHPLHLLHEGDEVQLRLPMFGG"
     gene            complement(5689..5892)
                     /locus_tag="P5516_07135"
     CDS             complement(5689..5892)
                     /locus_tag="P5516_07135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92244.1"
                     /translation="MDKEPSLFSRMSPADWLLAALFTAVVTALVHLTFPPHGWLIGML
                     IALGLLYLAKRRRDKLQPPRGGD"
     gene            complement(5938..>6954)
                     /locus_tag="P5516_07140"
     CDS             complement(5938..>6954)
                     /locus_tag="P5516_07140"
                     /inference="COORDINATES: protein motif:HMM:NF012798.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HRT92245.1"
                     /translation="QPLLPGELAQLIGLNANRAAAQARFHDYRSRRAEETLRRQDADL
                     ALFHEFLAFANLRTGDLANDPGAWRPVTWGLVEAFVKWQLKEGYAINSINVRLSTVKT
                     YARMAMQCGTLTPQEYALIRAVQGYSFREQVRIDQKRPVKRIGFKKEEPVRLTPEQAQ
                     ALKEQPPTPQGRRDRLLMCLLLDHGLRVGEAAGLAAPDLDADEGVLRFFRPKVNKEQA
                     HRLTRETLQAVRDCLANHELLPGELLLRRSKKNEELGAPGMSQRAITARVRLLGERLG
                     ILGLSAHDCRHYWATSAARHGTDPFVLQEAGGWSSLAMPRRYVEANDIANQGVRLEKG
                     EDEE"
     assembly_gap    6955..7054
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            7316..8353
                     /locus_tag="P5516_07145"
     CDS             7316..8353
                     /locus_tag="P5516_07145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92246.1"
                     /translation="MSVTPATQTRDTILEVKNLKTYFFTEDGVVKAVDGVDFSVKAGE
                     VLGLVGESGCGKSVSSFSILRLVGVPGKIVEGEIFFEGQDILKLSKDEMVKMRGDRIS
                     MIFQQPQSSLNPVFRVGEQVAEVLELHKGIKKKEAWARAVELLTLVGIPDPKKKAYAY
                     PHELSGGQAQRVMIAMALALSPKLLIADEPTTALDVTIQAQILDLINELREKTDTAVI
                     LITHDLGIIAETADRVAVMYAGEIVEFANVEPIFEKPLHPYTQGLIGSIPVLGNVVDR
                     LEVIPGLVPNLIDLPPGCRFEPRCLLRQKYGLEVCARREPELVEVTKNHKVRCWLYTD
                     SDEHSAPLKVE"
     gene            8355..9353
                     /locus_tag="P5516_07150"
     CDS             8355..9353
                     /locus_tag="P5516_07150"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HRT92247.1"
                     /translation="MSDQPGTNKINKQADLLVVNHLKKYFPVKTGVLKRVSGHLLAVD
                     DVSFSVKSGETLGLVGESGCGKTTIGRTILRLTEPTAGSVNFDGKEVFDLKGDALKAL
                     RRDMQIIFQDPYASLDPRLRIGNSVMEGLEIHKIGNKQDRYQMAVDMLKKVGLEDYHS
                     RRYPHEFSGGQRQRIGIARALTLKPRFIVCDEPVSALDVSIQSQVLNILKDLQDEFGL
                     TYLFIAHNLGVVEHISNRVAVMYLGKMVELADRDELFRSPLHPYTQALMSAIPIPNPK
                     LKRERIILKGDVPSPLNPPTGCRFHPRCPIAVAECALKEPTFVEKKPGHSVACWLA"
     gene            9493..11217
                     /locus_tag="P5516_07155"
     CDS             9493..11217
                     /locus_tag="P5516_07155"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT92248.1"
                     /translation="MKRRVFVLFSFLIVASLLLAACAAPTKAPVATEAPAATEAPVAP
                     PAAAAPLKIELAFRVVDRAYLPSPAKVAQEIQAELAEVGIQVTLKEMESAAFIDSTAA
                     GNEGFYLLGWNADYPDATNFYDYHFATAENKQFGALYDDMVAEIREAAKLSDPAARQV
                     HYDTINQMLKDYLMMIPVANGGSATVFAKDVEGAHSSPLGNEVFSVMSNGTDTLVWMQ
                     NGEPNALWCSDETDGETLRACEQIYEPLLAFAVGTADVTPALAVSYAANDELTEYTFT
                     LRDGVTFHNGATLDANDVVATYASLWDAASPNRTGRTGAFEYFGAFFGSTINSEAVEA
                     ELVDTDGMLSLTAPSCDYGGEFASIAAVDAKTVKFSLCYPDPAFPSKVAFSVFSILDK
                     EHLDATKGDSVAISDNPNGTGPYKLKKWNRGDSVVMEAYDGYWGEKAKTPNLIFRWSE
                     QSAQRLLELQAGTVDGIDNPGPDDIASIEVDANLALYPRNGFNIFYIGFNHDIAPFDN
                     LLVRQAIAYAIDRERIVEQYYPVGSSVSNSFVPAFVHPGASPDVLWWEYDPAKAKELL
                     AEAGYAVP"
     gene            11291..12367
                     /locus_tag="P5516_07160"
     CDS             11291..12367
                     /locus_tag="P5516_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92249.1"
                     /translation="MFQFIARRLMLLLPVMLGVVLVTFMIVRLIPGDPCVTMLGERAT
                     PAKCEAFLDRYGLNDNIGVQFVRYLQNLIKGDFGDSIRFTRPVTTLIAERLPLTMELT
                     LLAMIFSTTVGVLLGVISALKRNTFIDTITMVIANIGVSMPVFWLGLLLAYLFALTLK
                     GTPLSLPPSGRFSAGLSPIDLAKFWGLENLSGLKFFLVELMSNTVLVNVIITHDWKLF
                     WDALRHLILPAVAVGTIPMAIIARMTRSSLLEVVGLDYIRAARAKGMVERRVIGKHAM
                     RNAMIPIVTIIGIETGGLLSGAVLTETVFNLPGFGTGLVAAILARDYPVVQGFTLVIA
                     VIFVLVNLVVDISYAYLDPRIRLE"
     gene            12379..13305
                     /locus_tag="P5516_07165"
     CDS             12379..13305
                     /locus_tag="P5516_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92250.1"
                     /translation="MENITELNQIATSKPKSLFRESLRHLWHHSSGKAGIFILGMLIL
                     IAIFAPLIAPYNPTQILRESKRRTPPCIHLLGCPADQPQHLFGVDGNTRDLFSRVIYG
                     SRLSLQVGVVTMAFAILIGTLIGAVSAYAGGWLDNLLMRIMDVLMAFPSLLLAIAIVS
                     VLGTGLINALLAIGIVSIPRFARLVRSTVLTVRDLEYVQASRAMGASGLHILFKRILP
                     NALTPLIVQGTLGIATAILDAAALSFLGLGAEPPRPEWGLMLGEEKNSIFSAPHLVIF
                     PGIAIMFTVLGFNLLGDGLRDTLDPRLTHTAK"
     gene            13417..13884
                     /gene="bfr"
                     /locus_tag="P5516_07170"
     CDS             13417..13884
                     /gene="bfr"
                     /locus_tag="P5516_07170"
                     /EC_number="1.16.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004322 - ferroxidase activity [Evidence
                     IEA];
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006880 - intracellular sequestering of
                     iron ion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bacterioferritin"
                     /protein_id="HRT92251.1"
                     /translation="MKGKPKVIEMLNYLLADELTAISQYMVHAEMCDNWGYEKLAQTT
                     EKRAIEEMKHAEKHIARILFLEGKPIVSNLNKITIGPDVQTMLKNDHAAEEGAIKAYN
                     EAIKLCSSEGDNGTREMLEDILEDEERHIDWIEAQQDQIGQMGIQNYLVEQLG"
BASE COUNT         2801 a         3883 c         4061 g         2970 t
ORIGIN      
        1 aagaatcatg aatgaacagg gctatcggta catttatgac gctgatatcc tgctcagtat
       61 tcagagaggt atttacccgc aagccataaa ggcgcctgac atttttgagg aggtacctct
      121 tccatacctg ccatttttct tggttacttt tcaattattt tcgttaatcg atctaccttt
      181 tagctacctg acatggtctg ttctaaatat tattgtgttc tctttatatc tgaaattctt
      241 ttataaagaa attcatgctg aaacaataaa gtcgcaactt cttctgataa tgatgttatc
      301 tctccctgta tttctgaatt tgtattatgg acagttgaat atttggttag gaatatgtac
      361 tggtgaattc ataagggcga ttttctacaa gaaacctgtc aaagctggaa tttggttagg
      421 agggctgctc ttaaaacctc aattattgat tttattacta ccatttctat tgctcaaaag
      481 ggagttgaag gtcattgcgg gttttctgat ttcgtccatc ggaataggtt tgatttcttg
      541 gatattgatt gggagagaag ggtttttaga tttatttggc ttaatcgtta gttcttctca
      601 aggaggagga gccagtaacc cacaagccat gatgaatttc agaatggtag ggacttttgt
      661 ggagtatttc gtcaatgaga cgttcggaaa aatacttgtc gcggtttgtt cggcgataac
      721 cgctttggtg ttctttttga ttttttcgcg acaacaaaaa ttggataaat catcgattgg
      781 tttcacgagt ttgggtgtga tggcggctac ctgtgcagtg acctggcacg cgcatattca
      841 tatgtcaatt gtattgatac cccctttatt attcttgatt cacgatcacg cagttgaaag
      901 aacggtgaaa tattggttta tcctctttcc cactattttg cttatagtct atcttgttgc
      961 gggattggtt ggggcgggct tggttccacg aactgttatc ccgttttttg acctgtttct
     1021 cgggatggtg gggctggtat ttaatttgat tttcttgagg ttggcatctg ttggagcagg
     1081 caggatcaac cagaatattc tggataactc gcaacactta tccgcttgag atttaaaaat
     1141 caccccccgc tcttttccac cgcggccaac agggcctcgg cgaaggcgcg cgcctggggg
     1201 gcggtgagat acaggtggct ttcttcgttg cgcagcagcc gttcgctttc agcgcaatct
     1261 tcggtcagct ttaagcagat attatcctcc aggtcgtggt cttcgcaggc gtaaaagacc
     1321 tctactttca gcgtctcatc caatagaagg atcggtttca tcctcggcct ccttgtgttc
     1381 tcgttgttaa tagtatatgc aaaaccgccg cggcgttcaa ccggcaaccc ggaggggaga
     1441 tcggctattg tcgaaacttg ctttttgtga tgtaatttca tcaattctcc atgggagttg
     1501 ccatgcctgt cattttcttt aaactgaatt atcttgaccc cggttcgggc agcctgctcg
     1561 ttcaactcct cgccgccggc cttatcggcg gtatcggcat tttgcttaag atgttctggg
     1621 ggcggatcaa agccttcttc aaaggggaga agtacgcgcc gccgcaaccc gccgctgaag
     1681 aagagccccg ggaaacagaa ggggaatgaa ggctcaagct gagcgcagcg gggcgagctt
     1741 tcgcgacccg agcgggttcc tctttacgcg tgacggtgtt ttataccgcc aggtgaataa
     1801 agcataccag caggcgtatg aaaaactgat gagcggaggt gtttatcact cgctcaccct
     1861 gcagggtatg ctgatccggc acgaagaggt agacgcgccc gctgctgacc ccgcgctggc
     1921 ttacagggtc atccgcccgg aagaggtgcc cttcatttct tacccttatg agtggagttt
     1981 ctcgcagctc aaagacgccg cgctgctgac gctggcctgc gcgcgcgccg cgctggaaaa
     2041 cggtatggtg ctcaaagacg ccagcgcgta caatatccag ttcgtcaatg gaaaaccgct
     2101 gctcatcgat acgctctcct tcgacctcta ccaggagggc gccccctggg acgcctaccg
     2161 tcagttctgc cagcacttcc tggcgcccct ggcgctggct tctatggtgg atatccgcct
     2221 gacccagctc ttgcgcgttt acctggacgg cattccgctg gatctggcca gcgtgctgct
     2281 gccgggcaag accaggctgg ggttcagcgg gctggcggtg cacctgcacc tgcacgcccg
     2341 ggcgcagcgg cagtacagcg acaggcaggc cgcccaggcc gcggcggtga aattgagcaa
     2401 gacggcgctg ctgcacatcc tcaacgggct ggaaaaaacc atcctgccgc tcacctggca
     2461 gccgcgcgga accgaatggg gcgcgtatta cagcctcact aattacacag atgaatctct
     2521 ccaggccaag ggcgcgctgg tcggcagctt cgcggagcag gttaaaccgc gcagcgcctg
     2581 ggacctgggc gccaacaacg ggttgttcag ccgcgagctt tcgcgccgcg gcatcgaaac
     2641 cgtcgccagc gatatcgacc cggccgcggt ggaacagaat tacctggcgg tgaaggccgg
     2701 caatgaacct cacctgctgc cgctgctcat tgacctgacc aaccccagcc cggcgctggg
     2761 ctgggcgcac cgcgagcgca gctccatgct ggcgcgtgga ccggtggacc tggtgatggc
     2821 gctggcgctc gttcaccacc tggcgatttc aaataacgtg ccgctgcccc tggtggcgga
     2881 gtttatggcc aacgccggcc gctgggcgat cgtcgagttc gtgcccaaga gcgacagcca
     2941 ggtgaaacgc ctgctgagca cgcgcaagga tatatttgac cagtacaacc aggaaggttt
     3001 tgaagaggct tttgcccttt actttaacac ggagcgcaag gcgcccatcc cgggcagcca
     3061 gcgcacgctc tacctgttca aacgcaagaa ttaattcacg ctgaacggct ttcaggcgct
     3121 ggtgagccag cccagcgcgg gcatggcagc caggttggat ggctgttcat tcacgcggtt
     3181 tgaacagccc ctctgttcag tttttgaggt tgaaaaccgg gcggatctcg ccaatcggga
     3241 tgtttcgcca gttggtgagg gcgcgcgggg cgaagtcgct caattcttcc tgttgcgcgc
     3301 tgtcgagcac gcccagttgg cgcagggctt caagcgcggc agtagagcgc gcgcgcccgc
     3361 cgccgcgggg ggcgatggcg ttggcgcgct gaccgcggtc agcctgcgcc aggtcgccgt
     3421 cgctgatctt gagggtgatg cccatggcgc cgctggagcc agccggcagg accgccaggg
     3481 cctggtagcc ctcggcgccg cccttggtga gcacccgccc gcgcccggcg gtcatcaggc
     3541 gggtgtcgaa ggtatccggt cccgcgacca tatcggggtg ggaggtcatg gcgctgaaga
     3601 tgcggcgcag cgcggagcgg cgcggttcgg gcagcgcgga cgggtcagcc aggcgggcga
     3661 aagcgtaagc agcggcgcgc aggggcaccg cgaacaccgg cgcggaacag ccgtcagtgc
     3721 ccaggatgat ctccggtacg ggcacgccgg tcatttcact gaacacacgc agaatgcgct
     3781 gctgcacggg gtgacctggg tcgatgtaat cttgccgggg atcgccgtaa agcagcgcct
     3841 gtgccagcat gccggtgtgt ttgcccgagc agttgtggcg cagggggctg ttttcttcgc
     3901 cgcgcaagaa catgcgttca gcggtgagcg ggtcggaggg ggtgtgcgtg ccgcacaaca
     3961 gctcagccgc gctgaccccc agcttgcgtt gcagcgccgt gagcaccgcc acatgctcat
     4021 ccgtgccgct gtgcgaggag cacatcagcg ccagctcgcg gtcgttcagg ccgaagcgct
     4081 ccatcccgcc gccttccacc agcggcagcg cctgaaaagg cttggaagac gagcgcagga
     4141 agaccagcgc ttcagcgtcg cccgcgcgcg ccagcaaccg gccctgtgtg tcgcacacgg
     4201 cgatggagcc gtaatggatg gattcgacaa taccgccgcg cgtggtttct accaggggga
     4261 cataatgagg gttgggcata aggtttcctc aggttggttg gtatagatca attttacacc
     4321 cgaggggtgc ccgcaaggga cagagaagcg gcccgcgcgc ggagagttga agggcgcgcc
     4381 gcaaggctgc ggtgcgtaaa gcgggagagg atgcgcatgg gtcgagcgct tttatcaggt
     4441 atacttgcac acaaaaaaaa gagggtaaat atgatcgaag aaagaccgtt acacgttctg
     4501 ggcatcagcg gcagcctgnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4561 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnngg
     4621 taaatatgat cgaagaaaga ccgttacacg ttctgggcat cagcggcagc ctgcgcgttg
     4681 actcttacaa caccgcgctg ctgcgcgccg ccgcggaact actgccgccc ggtatgacac
     4741 tggaaatcac cgacctggcc ggcatcccgc tttttaacca ggatgaagaa aatcctttcc
     4801 ccgaagaggt tgcccgtctg cgggcgcgca tcgcggcggc agacgcgcta ctgttcgcca
     4861 cgccggagta taacagctcc atcagcggtg tgcttaagaa cgcgttggat tgggcttcgc
     4921 gcgcgccgga tatgccgctg gccggcaagc ccgcggcgct gatgggcgcc agcacgggcg
     4981 tattcggcac agcccgcgcg caattacacc tgcgccaggt gctcgctcac ctgggcgcgc
     5041 tggtgctgac gaaacctgag gtgatggtca tgcgggcagc ggacgccttt gatgaacagg
     5101 gcaggttggt gaacgaagtg acacgcggtt ttttgggtca attgctcgtt gaactggcgc
     5161 aatggacgcg cctggtgagc gctcgttgaa ccgaagagaa gagtagaaca gcatgaaaac
     5221 agactgccgc cggaggagcg ctcctccggc ggcagcgatg aatcacgctt ttcaccgccc
     5281 gtggggaatg gttttcaccc gccgaacatg ggcaggcgca gctgcacttc gtcgccctcg
     5341 tgcaacaggt gcagggggtg caccatctcg ttttggatga tgacctgcgc gttgcgcaaa
     5401 aaggggcgta tcacggggta ccgcgccgcc agcgtgtcga gcatcagcgt agcggcggcg
     5461 ccttcgggca gcgtcagctc caggcgcgat tccccgcaaa actctttgag cggcgaatac
     5521 aactgcacat gaacattcat caccgttcac ccatttaatt gattatatgc gatttgccgt
     5581 gagagctgcg tggagaggat ggaaccaggg cgcggtgcac ggtcacgcgc tcgtgagcga
     5641 tcgtgcggcg ggcgaaagca gggttgaacc gtgtaaaaag cggcggcgct aatcaccccc
     5701 tcgcggcggc tgcagcttat cccggcggcg cttggccagg tagagcagcc ccagcgcgat
     5761 gagcatgccg atgagccagc cgtgcggcgg gaaggtgagg tgcaccagcg ctgtcaccac
     5821 tgcggtaaag agggcagcca gcagccaatc tgccgggctc atgcgcgaaa agagagaggg
     5881 ttctttatcc attccagcct tggtctcctg aagcgcgcgt aaagcgcgcg cgatttttta
     5941 ttcttcgtct tcgcctttct ctaatctcac cccctggttg gcgatgtcgt tggcttccac
     6001 gtaacggcgc ggcatggcga gcgagctcca gccgccggcc tcctgcagaa cgaaggggtc
     6061 ggtgccgtgg cgcgcggcgg aggtggccca gtagtggcgg cagtcgtgcg ccgaaagccc
     6121 caggatgccc aaccgctcac ccagcaggcg cacgcgcgcg gtgatggcgc gctggctcat
     6181 gccgggggcg cccagctctt cgttcttttt gctgcgccgc aacaacaact ctccgggcaa
     6241 tagctcgtgg tttgccaagc agtctctcac cgcctgcagc gtctcgcggg tcaggcggtg
     6301 cgcctgttcc ttgttgacct tggggcggaa aaagcgcagc acgccctcat ccgcgtcaag
     6361 gtcgggcgct gccagcccgg ccgcttcgcc cacgcgcagc ccgtggtcga gcagcaggca
     6421 catcaacagg cggtcgcgcc ggccctgcgg ggtagggggt tgctctttga gcgcctgcgc
     6481 ctgttcgggg gtaagcctca ccggctcttc ttttttgaaa ccaatgcgtt tgaccgggcg
     6541 cttctggtcg atacgcacct gctcgcggaa tgagtagccc tgcacggcgc gtatgagcgc
     6601 gtactcttgc ggcgtcaagg ttccgcactg catggccatg cgcgcgtaag tcttcaccgt
     6661 ggaaaggcgc acattgatac tgttgatcgc gtaaccttct ttcaactgcc atttcacaaa
     6721 ggcttccacc agcccccagg ttaccgggcg ccaggcgcca gggtcgttcg ccaggtcacc
     6781 ggtgcgcagg ttggcgaagg cgaggaattc atgaaagagc gccaggtcgg cgtcctggcg
     6841 gcgcagcgtt tcttcagcgc ggcgcgagcg gtaatcgtga aagcgcgcct gcgcggcggc
     6901 gcggttggcg ttcagaccaa tgagctgagc cagttcgccg ggcagcaggg gttgnnnnnn
     6961 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntaactg tattttgccc gatcaggcga
     7081 gtacggctga tcaccagagg ctaatcagtt ttgtttagct ttactcaaac gtaaccccaa
     7141 ccctgagagg tccaggttcc cgcgcctccg ctgtgaagga gcgggaactg cgcaaaggct
     7201 tgaaagccag tataatggta tatcgatttc ccagcacgcc attatcggta gcctacaggt
     7261 aaactcacag tcattgcagc gtgagcatcc attttttgga tatggagagc attgtatgag
     7321 tgtaacgccg gccacacaaa caagggatac cattttggag gtgaagaacc tgaagaccta
     7381 cttcttcacg gaagacggtg tggtcaaggc ggtcgatggc gtggatttct cggtgaaagc
     7441 cggagaggtg ctggggctgg taggagaatc agggtgcggt aagagcgtca gttcattctc
     7501 cattctgcgg ctggtgggcg ttcccggtaa gatcgtggag ggcgaaatct tcttcgaagg
     7561 gcaggatatc ttaaaactct ccaaagacga gatggttaaa atgcgcggcg accgtatctc
     7621 gatgatcttc cagcaacctc aatccagcct gaacccggtg ttcagggtgg gggagcaggt
     7681 ggcggaggtg ctggaactgc acaagggcat caagaaaaag gaggcctggg cgcgcgccgt
     7741 tgagctgctc accctggtgg gcattccaga cccgaaaaag aaagcctacg cttatccaca
     7801 tgagctttca ggcggacagg cgcagcgcgt gatgattgcc atggcgctgg cgctttcgcc
     7861 caaactgctc atcgccgatg aacccactac cgcgctggat gtgaccattc aggcgcagat
     7921 cttagacctg atcaacgaat tgcgcgaaaa gacggatacc gccgtcattc tgatcaccca
     7981 cgacctgggc atcatcgcgg aaaccgccga ccgcgtggcg gtgatgtacg ccggcgaaat
     8041 tgtggagttc gccaacgtgg aacccatttt tgaaaagccg ctgcaccctt acacccaggg
     8101 gcttatcggc tccatcccag ttctcggcaa cgttgtcgac aggttagagg tcatccccgg
     8161 gctggtgccc aacctcatcg acctgccacc gggctgtcga tttgagccgc gctgcctgct
     8221 gcgccagaag tacgggctgg aggtttgtgc caggcgcgaa cctgaattgg tggaggtgac
     8281 caagaaccac aaggtgcgtt gctggctgta cactgactct gatgaacata gcgctccttt
     8341 aaaggtcgaa tgatatgagc gatcaaccag gcacgaacaa aataaacaag caggccgacc
     8401 tgttggtggt caaccacctg aaaaagtact tcccggtgaa aacaggcgtg ctcaagcgcg
     8461 tctccgggca cctgctggcg gtggatgatg tgagttttag cgtgaagagc ggcgaaacgc
     8521 tggggctggt gggcgaatca ggctgtggca agaccaccat tggccgcacc attctgcgcc
     8581 ttacggaacc tacggctggt tcggtgaact ttgacggcaa ggaagtgttt gaccttaaag
     8641 gagacgcgct caaggcgttg cgtcgtgata tgcaaatcat cttccaggac ccctacgctt
     8701 cgcttgaccc gcgtctgcgc atcggtaatt cggtgatgga ggggctggag atccacaaga
     8761 ttggcaataa gcaagatcgc taccagatgg cggtggatat gctgaagaag gtggggctgg
     8821 aagattatca cagccgccgc tacccgcacg agttttcagg cggacagcgc cagcgcattg
     8881 gcatcgcccg cgcgctgacg ttgaaaccgc gctttatcgt ttgtgatgag ccggtctcag
     8941 cgctggatgt ttccatccag tcgcaggtgc tcaatatcct caaagacctg caggatgagt
     9001 ttgggctgac ttacctgttc atcgctcaca acctgggcgt ggtggagcat atctccaaca
     9061 gggtggcggt gatgtacctg ggcaaaatgg tggaactggc tgaccgtgat gaactgttcc
     9121 gcagcccgct gcacccgtac acccaggcgt tgatgtccgc cattcccatt cccaacccca
     9181 agttgaagcg cgagcgcatc atccttaaag gagatgtgcc cagcccgctc aacccgccca
     9241 cgggctgccg ctttcacccg cgctgcccca tcgctgtggc ggaatgcgcg ctcaaagaac
     9301 cgacgtttgt ggagaaaaaa cccggccaca gtgtggcctg ctggctcgcc tgaaggcgcg
     9361 ccgctatcgg ataagatgtg aggagcaagt ctaaaaggag gtggttttat tcagccataa
     9421 ccagcgccag tgaccaaggg ttttcttgtc gagaacccgt aaacgttcaa tcaatcaaca
     9481 aggagaaaag aaatgaaacg tcgtgtgttt gtattgttca gctttttgat cgtcgccagc
     9541 ctgctgctcg cggcctgcgc ggcgccgaca aaagcgcctg ttgccacgga agccccggcc
     9601 gccactgaag caccggttgc gcccccggct gccgccgctc ccctgaagat cgaactggcc
     9661 ttccgcgtgg tcgaccgcgc ctacctgcct tcacccgcca aggtagcgca ggagatccag
     9721 gcagagttgg ctgaggttgg tattcaggtg accctgaaag aaatggaatc cgccgcgttt
     9781 atcgattcca ccgccgctgg taacgaaggc ttctacctgt tgggctggaa cgccgactac
     9841 ccggacgcca ccaacttcta tgattatcat ttcgccaccg ctgagaacaa gcagtttgga
     9901 gcgctttatg atgacatggt cgcagagatt cgcgaagccg ccaagctttc agaccccgca
     9961 gcacgccagg tgcactatga caccatcaac cagatgctca aagactacct gatgatgatc
    10021 ccggttgcca acggtggctc ggccactgtc ttcgctaagg atgtcgaagg ggcgcactcc
    10081 agcccgctgg ggaacgaagt cttcagcgtg atgagcaacg gcaccgatac gctcgtgtgg
    10141 atgcagaacg gcgaacccaa cgcgctgtgg tgctcggatg aaaccgacgg cgaaaccctg
    10201 cgcgcctgcg aacagattta cgaacccctg ctcgccttcg cggttggcac ggctgatgtg
    10261 actcctgcgc tggctgtgag ctacgcggcg aacgatgaac tgacggagta taccttcacc
    10321 ctgcgcgatg gcgtcacctt ccataacggc gccaccctgg acgccaacga tgttgtggcg
    10381 acctatgctt ccttatggga tgccgccagc cccaaccgca ccggccgcac tggcgctttt
    10441 gagtattttg gcgctttctt cggcagcacc atcaattccg aagcagtgga agccgaactg
    10501 gtggataccg acggtatgct ttcgctgacc gccccgagct gcgattacgg tggtgagttc
    10561 gcctccatcg cggctgtcga cgccaagacg gtcaagttca gcctgtgcta ccctgacccc
    10621 gccttcccgt cgaaggtcgc cttctcggtc ttcagcatcc tggataagga gcatctggac
    10681 gcgaccaagg gcgattccgt ggccatcagc gacaacccca acggcaccgg tccttacaaa
    10741 ttgaagaagt ggaaccgtgg cgacagcgtg gtgatggaag cttacgatgg ttactggggc
    10801 gagaaagcca aaacccccaa cctgatcttc cgctggtctg agcagtccgc gcagcgcctg
    10861 ttggaactcc aggctggcac ggtggacggc atcgacaacc ccggtccgga tgatatcgcc
    10921 tccatcgagg tggatgccaa cctcgcgctg tacccccgca acgggttcaa catcttctac
    10981 atcgggttca atcacgatat cgcccccttt gacaacctgc tggtccgcca ggcaatcgct
    11041 tacgcgattg accgtgagcg cattgtagag cagtactacc cggtgggctc cagtgtttcc
    11101 aacagtttcg tgcccgcctt cgtgcaccct ggcgccagcc cggatgtgct gtggtgggaa
    11161 tacgatcccg cgaaagccaa agaacttctg gctgaagccg gatacgctgt accctaagct
    11221 ttaacctgtt cgtgtggatg gggctggtta aacagcccca tccattatcc ttcacttaga
    11281 gagacgcgtt atgttccaat ttatcgcccg taggctgatg ttgctgctgc cagtgatgct
    11341 gggggttgtg ctggtcacct ttatgatcgt gcgcctgatc ccgggtgacc cttgcgtcac
    11401 catgctgggg gagcgcgcca cccctgccaa gtgcgaagca tttcttgacc gctacgggct
    11461 gaacgacaat atcggcgtgc aattcgtgcg ctacctgcag aatctcatta agggtgattt
    11521 cggtgattcc atccgtttca cccgaccggt caccacgctc atcgctgagc gcctgccgct
    11581 gacgatggag ctgaccctgc tggccatgat tttttccacc acggtggggg tgttgctggg
    11641 ggtcatctcg gcgttaaaac gcaatacctt cattgacacc atcaccatgg tcatcgccaa
    11701 tatcggcgtc tccatgccgg tcttctggct ggggctgctg ctggcctatt tgttcgcgct
    11761 tactttaaaa ggcacacctc tctcgctgcc tccctccggc aggttctcgg cgggcctctc
    11821 tcctattgac ttggcaaaat tctggggtct tgaaaacctc agtggcttga agttcttttt
    11881 agtggaatta atgtcgaata ccgtgctggt gaatgtgatc atcacccacg actggaagct
    11941 tttctgggac gcgttacggc atctcatcct gccggcagtg gcggtgggca ccatcccgat
    12001 ggcgatcatc gcgcgcatga cgcgctccag cctgctcgaa gtggtgggtc tggattacat
    12061 ccgcgccgcg cgcgccaaag gcatggtgga acgccgcgtc atcggtaagc acgccatgcg
    12121 taacgccatg atccctattg tcaccatcat cggtattgaa accggcggcc tgctttcagg
    12181 cgcggtgctc accgaaacgg tgtttaatct tcccgggttc ggcaccggcc tggtggcggc
    12241 tattttagcg cgtgattatc cggtggtgca gggctttaca ctggttatcg cggtcatttt
    12301 tgtactggtg aacctggtcg ttgatatctc gtacgcgtat cttgatccgc gcattcgatt
    12361 ggagtaggtg gagccgatat ggaaaacatc accgaactga atcaaattgc cacctcgaaa
    12421 cccaaatcac ttttccgtga gtctctgcgc catttatggc accatagttc aggcaaagcc
    12481 ggcatcttca tcctgggcat gctgattctt atcgccattt tcgcgccgct catcgcccct
    12541 tataacccca cccaaatcct gcgagaatca aagcggcgca ccccgccatg cattcatctg
    12601 ctcggctgcc cggcagacca gccgcaacac ctgttcggcg tggacggcaa tacgcgggac
    12661 ctgttttcgc gggttattta cggttcgcgc ttatcgttgc aggtcggtgt ggtcaccatg
    12721 gcttttgcca tcctgatcgg cacgctcatc ggcgcggtct cggcgtacgc cgggggctgg
    12781 ctggataacc tgttgatgcg cattatggat gtgctgatgg cctttcccag cctgctgctg
    12841 gcgatcgcca tcgtttctgt tttaggcaca ggtctcatta acgccttact ggcgattggt
    12901 atcgtctcca tcccgcgctt cgcgcgcctg gtgcgttcta ccgtgctcac cgtccgcgac
    12961 ctggagtatg tgcaggcgtc gcgcgccatg ggggcttcgg gtttgcatat tttattcaag
    13021 cgcattctgc ctaacgcgct cacgccgctc atcgtgcagg gcacgctggg cattgccacc
    13081 gccatccttg atgccgccgc gctctcattc ctcggtttgg gggcagaacc gccgcgcccc
    13141 gagtggggtc tgatgctggg tgaagagaag aacagcatct tcagcgcgcc gcacctggtg
    13201 atcttccccg gcatcgccat catgttcacc gtgctgggtt tcaacctgtt gggcgacggc
    13261 ctgcgcgata cgcttgaccc gcgcctgacg cacacagcca aatgaacgcg gaaaatccgg
    13321 agaaaccctc cggatttttt gatgcaaacg ctttaccgct gacggaagtt ctgcgcgtgg
    13381 gcgggattga tttataatta aatcacggag gcgaaaatga aagggaaacc gaaagtgatt
    13441 gagatgttga actacctgtt ggccgatgag ctgaccgcca tcagccaata catggtgcac
    13501 gcggagatgt gcgacaactg ggggtacgaa aaactggcac agaccaccga aaagcgcgcc
    13561 attgaagaga tgaagcacgc cgaaaagcac atcgcgcgca tcctgttttt agaaggcaag
    13621 ccgatcgtca gcaacctgaa caagatcacc attggccctg atgtgcagac catgctcaag
    13681 aatgaccacg cggcagaaga aggcgccatc aaggcctata acgaagccat caagctgtgc
    13741 agcagcgagg gggataacgg cacgcgtgaa atgctggaag atattttaga ggacgaagaa
    13801 cgccacatcg actggatcga agcgcagcag gaccagatcg ggcagatggg cattcagaac
    13861 tacctggtgg agcagttagg ctgaccaggc gttctccagt gtagacaacc aaagg
//
LOCUS       DAOTLW010000044        13488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_5010_length_13488_cov_11.040200, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000044 DAOTLW010000000
VERSION     DAOTLW010000044.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 13488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13488
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5010_length_13488_cov_11.040200"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(266..943)
                     /gene="nth"
                     /locus_tag="P5516_07175"
     CDS             complement(266..943)
                     /gene="nth"
                     /locus_tag="P5516_07175"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140078 - class I DNA-(apurinic or
                     apyrimidinic site) endonuclease activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HRT92252.1"
                     /translation="MPPTSPAEQIRSVLQVLKRHYGAPVWRDPLPAIDELVSTILSQN
                     TNDSNRDRAFNTLRQTFADWDAVRTAPDEEIIAAVRVAGLANQKGPRIKAVLNQIHEE
                     VGSLDLSFLAEMPLEEARAWLLRFKGVGRKTAAIVLQFALGHPAFPVDTHIYRVTGRL
                     GVRPATMSVEKTHLLMEELIDPADFYAGHLNLIRLGRETCHPRKPDCPRCPVRELCLY
                     PDKTVEP"
     gene            complement(967..1827)
                     /locus_tag="P5516_07180"
     CDS             complement(967..1827)
                     /locus_tag="P5516_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92253.1"
                     /translation="MQEIAPQIYIEREYPGVTLGLINWKRSLILIDAPFRPEDIRLWR
                     VSMHELAPNTDRLLISLDDHYDRTLGSRQIEGPVIGHEKLTQLFKDRPVNIKPQGIET
                     GAAWEQHNTLGNIRWSVPEITFTEHLEIHSDAHTLLLDSHPGHSPAAIWVTLPEEKVC
                     FIGDTVTPHSVPFLANADLAQWRDTLSLLLKPVYRSYILVSGRGGLISTNDIKEQLKI
                     LEKINKQLEKLGGKPLRNEDIQKTAANILKQCNVPKEEEQQHLQRARWGLVQYLKHST
                     GSHPEQPAVL"
     gene            complement(1831..3417)
                     /locus_tag="P5516_07185"
     CDS             complement(1831..3417)
                     /locus_tag="P5516_07185"
                     /inference="COORDINATES: protein motif:HMM:NF024883.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT92254.1"
                     /translation="MSITEDHAQLEILYNVSRQLAASLDLHEVLARVLLLSTSNLGAE
                     RASLIVLDNAHKPCDAVILYKGELSSASHETINAIVRYGLAGWVIHNRQPVILENTRA
                     DARWLERPYEKETAGSPKSALCLPVHAREKIVGVLTIVHPLPGNFNEAHLKLQQAIAD
                     LAGVAIHNARLYEDAKHSRNLYRGLFEGSPDPIFITTLDGQIIDCNRQGIFVSGFDRR
                     FLLRSSVIHLDPLASDVLTRAGAGRKRSSAGDYESSLSIKGQQRLPVEVSAVRVPYRD
                     LDCLQWIYRDTSARHELDALRDNLSAMIYHDLRSPLANIISGLELLAETIPPEAGPDA
                     QQLLQIAGRSSAHMQRLVSSLLDINRLESGQELTRLRRSKLKKLLDESIEVISPKVRS
                     KKLEVVKQVPRKVPVVNADEDMIRRVIINLLENAARYSPPGSKITTRIQAQAHQVVVF
                     IEDEGTGIPEEAKERVFEKFARIDDESAVRGIGLGLAFCRLAVQAHGGKIWVENLHAG
                     GARFAFSLPLSQRQELTPEG"
     gene            complement(3404..4591)
                     /locus_tag="P5516_07190"
     CDS             complement(3404..4591)
                     /locus_tag="P5516_07190"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HRT92255.1"
                     /translation="MNPSPSQRFTLSCPECGRAFDPLLPQTYCHDCNSPLLSHYPLER
                     LKAELRREEISARPRGMWRWHELLPVEDPHNRVSLGEGDTPLLPLPTIAAALGASNLL
                     LKDESLNPTGTFKSRGMAAAVSKALELGIREFVIPTAGNAGGALGLYAGRAGCAAHVF
                     MPKDTPAPIQYEIRMSGADVHLVDGLINDAARLAAAESRAHGWFDVSTFKEPYRVEGK
                     KIMGYELAEALGWRLPDVIIYPTGGGTGLVGMWKAFDEMQSLGWIDSARPRMVSVQAA
                     GCAPVVRAFEEGAQRIQPWQNAATIAAGLRVPAVFADRLVLRCLYQSGGCAVSVSDEE
                     LTLAQHELARAEGILAAPEGAATLAALKILLKKGWCEYGESIVLFNTGTGLKYFQQDH
                     VDH"
     gene            complement(4688..5392)
                     /locus_tag="P5516_07195"
     CDS             complement(4688..5392)
                     /locus_tag="P5516_07195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HRT92256.1"
                     /translation="MKRFFFLLGSGVCIAVIVAVYFLWVNTSLFNPRSRDLMKWLRDP
                     AAHPDWRIEAGQRCDGALFSLPTSGYIGYLWNDSFRPGHHHQGIDIFGGGEPGSVPVY
                     AAYDGYLTRLADWKSTVIIRIPADPLQPQRQIWTYYTHMAYPSGESLIAAQFPPGSSE
                     VFVKAGTLLGYQGNYSGTRDKPTGVHLHFSIVKDDGNGRFLNELEIDNTLDPSPYLGM
                     ALNNKAGATTPAVCTP"
     gene            complement(5397..6710)
                     /gene="miaB"
                     /locus_tag="P5516_07200"
     CDS             complement(5397..6710)
                     /gene="miaB"
                     /locus_tag="P5516_07200"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HRT92257.1"
                     /translation="MKYHVWTEGCQMNVADSERVSSALEHLGYTHTSNAEDADVIVLN
                     TCVVRQSAEDKAYGRLTSLLPIKRDRPEVVINLMGCLVGIKDHQPLRQRFPYVDVFSP
                     PSDPAPLVEYLIQRDARSAEEAETARRFALMDGDLVLPRSSGGQVAGWIPVVYGCSHA
                     CAYCIIPYRRGVEVSRPPEEIYREARSLAAQGVKEITLLGQIVDRYGKDQPAYPTLAG
                     LLRELSGIEGIERLRFLTSHPNWMTDELLECVAELPKVMPHIEVPNQSGDDDVLQAMK
                     RGYTAEDYRRLVQKIRDRIPGVSIATDIIVGFPGETEAQFQNTYDLLAELKLDVVHLA
                     RYSPRAGTLSARSLEDNVPETEKWRRFRALERLQEAIVREIHATYLHTTVEVLFEDKV
                     KKRWRGRTPTNKLVFVETETNLRGMVLPVKIIRTGPWSMLGELVS"
     gene            6855..8264
                     /locus_tag="P5516_07205"
     CDS             6855..8264
                     /locus_tag="P5516_07205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92258.1"
                     /translation="MNKKIGVTLLVLIISSCVLLSAGVIIGAYLLLKAQKNYTDPLLT
                     LRNTPAVATVTAAQQTQAAAPSDGLPPKIAKQMDEIQQQVIAIRGLHMTTDLKRDLLT
                     PEELQDKVINDFFADYSDEDALKDSKVLSTLGLLDEGFDLKQFYLDLYSEQIAGYYDS
                     ETKDMYVIAGEGFGGMERMTYAHEFNHVLQDQNYDLENGMKLNDDYCKVDTEYCAAVS
                     ALIEGDSVSTENEWFLKHSTRQDQKDLFSFQEEYTSPVYDSAPAYMKEDFLFPYTFGL
                     DFVQQLINQGGWAAVDAAYANPPVSTEQILHPEKYPDDRPVPVEMPDLAGMLGDDWSE
                     LDRNVMGEWYTYLVFAKGRSSQFSMDDEKSKTAAAGWGGDTYVYYATADLKEHLFAWR
                     STWDTSSDAGEYFDMSRDYGLARWGIPVQNSNGVISWNSTSDGIITMRRSGPDVLWVI
                     GSSKEVVDDALTEIKDFGK"
     gene            8267..8956
                     /locus_tag="P5516_07210"
     CDS             8267..8956
                     /locus_tag="P5516_07210"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024811.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HRT92259.1"
                     /translation="MPLDLQRIKGLCFDVDGTICDTDDAWVEKITDFFQPVAKIVKCQ
                     KPRQFARWLVMAAESPMNLVMYWLDTLSLDDNFARLFTRMVKRREYKNTAFRLMRGAN
                     ELLTMAEKKYPLAVVSARDEKTTMMFLEQFSLSKHFVEVVTSQTCERTKPYPMPVQHA
                     ANAMGLQARDCVMIGDTTVDILAGRRAGAQTVGLLCGFGTEKELKKAGADVVLNDLEE
                     LQALLFPVGGN"
     gene            complement(8953..>10470)
                     /locus_tag="P5516_07215"
     CDS             complement(8953..>10470)
                     /locus_tag="P5516_07215"
                     /inference="COORDINATES: protein motif:HMM:NF037015.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRT92260.1"
                     /translation="HGAVNMRSALANDYLTPLAKIVNETGAANTWRSAAYIGLDSWEN
                     PPDNASPLYGGSPVSLLEVGSAYSTLANAGVRSGRLDKATNRIKPVTVLRVNTSRHQT
                     IWQAPLAENAVIISESVSYLVNHVLSDESARWPSLGHPNELEIGMEAAAKMGRADNGH
                     QVWTVGYTPQRLVLVWMGSRSTEKDTPGLDKRMAAGIWHAVIKYASRGLEHKGWQRPA
                     GITQLQVCSPSGMLPTMDCPNLVYDYFLYGNEPTLPDTLFTRFKVNRETGQLATVFTP
                     PELIDEKVFMNVPAEARLWAQSAGIELIPQGYDAIQTIRQDELVNITQPLMFSAVAGK
                     VVIRGTAAAAHFASYSIQVGEGINPESWLRVKESTRSLSEDGVLAEWDTSGLNGLYAI
                     RLTVIDADNAVRSAVTQVTVDNTPPSARITYPLPDQHITPVRGEVTITAQVEDLVGVA
                     RVEWWVDGKRVSEQTAAPYLYQLSESAGKHSVYLKAWDVAGNSIQSEKISFSVAP"
     assembly_gap    10471..10570
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<10571..11524)
                     /locus_tag="P5516_07220"
     CDS             complement(<10571..11524)
                     /locus_tag="P5516_07220"
                     /inference="COORDINATES: protein motif:HMM:NF013106.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HRT92261.1"
                     /translation="MRKTSLYLLLKHRSHSRQRHTARASRAFTWLARALAGVVSLVIV
                     AALLIAGYFYALVARDLPSIEILPSLMDRENGELLQPTRLYDRSGVVTLFAYSNEGIE
                     RKFLPVNPDNPEFISPQLVRSAITAIDPTFWSNPGYLLKEWHTVEPLTIAERLVSELI
                     LWDEPPSITRAVRMRILAGQVVSTYGRTRALEWYLNSAWFGRNAYGVESASRLYLGKS
                     AQQVTLAESALLISLLKSPALTPFDAPEAAFNSQKELLRSMRAVNVISPEEYEQALNE
                     VIHLRAAKKPVEVAENGFVKIVEKELESILPAQRLRRGGLEV"
     gene            complement(11531..13357)
                     /locus_tag="P5516_07225"
     CDS             complement(11531..13357)
                     /locus_tag="P5516_07225"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT92262.1"
                     /translation="MAKDAIEQGENAAAREHLVRLLQFDRSNPQYWLWMSAVVETLKE
                     REFCLREVLKLDRKNPTAIHGLRILGYAIEPPEMPPGFDPLKRDWKTSLEIEKEVEQK
                     TLKRKPRTSSWVMLGALVFSISALVFFLVESNRYRPDTSPILKFSLTPPYSATLTSTP
                     TPFTTGAPPLWTLLEATYTPTPIYAATPHKLSEAYMAAMRAYTKGDWVNALVFFQQVV
                     DAEPDSADIWYHIGEVYRFQGMLKAADTAYDNSLKSNVAFAPAYLGKARVSMSSASPD
                     HKHAAQYLQKALELDPGLSEALSALAELQLSEGDAVAALNSLSQYEERFSLSAQVELL
                     RARAYLISGNAKEALSAAQHANRLDITLLEAYKLIAAAMQNNEQVAGSIEPLETYLTY
                     ETNDAEALSMMSLALVEAGNLQEAFLYAERALDIDQRSVPALIARGRVLFEQGAYLEA
                     AETLDSAIEIQETSFEANILKSQVQLTRKLNDSAIEFSRRAYELAKNDRQKATALYWR
                     ARAYAAKKQNDSARADLKALLELPVENLPADLREEARKLLHQLSAATSTPTTSQTRTT
                     TRTPSASATPAPTQSATFSVTRTPTPSPAGSSAQTSSPTPRR"
BASE COUNT         2924 a         3568 c         3864 g         3032 t
ORIGIN      
        1 gcgcaggttc caatccgttt gcccgtgcct gaccagccac atttccgtca taagattgga
       61 ttataactga tgaaaaatcg cgggttggat cgtttttcgc cagttcattt ggacgtgcgc
      121 cagaacaaaa taacctggct ttggtatcct ctggtgaagg aagaagaacg gcaggagcga
      181 aggtgtattt cgataagaat gtgttgtgaa ccgtattttt acaaaggagg aaaggacaaa
      241 gtgaggtaaa aaccgcgccc ggggtttacg gctctacggt cttatcgggg taaaggcata
      301 attctctcac cgggcagcgc ggacaatcgg gtttgcgggg gtggcaggtt tcgcgaccca
      361 ggcggatcag gttcaggtgc ccggcgtaaa aatcggcagg gtcgatcaat tcttccatca
      421 acaggtgtgt tttttccacg ctcatggtgg cgggtctcac ccccaggcgg ccggtgacgc
      481 ggtaaatgtg cgtatctacg gggaaggcgg gatgccccag cgcgaactgc agcacaatgg
      541 cagctgtttt gcgccccacc cctttaaaac gcagcagcca ggcgcgggct tcttccagcg
      601 gcatctccgc caggaaggaa aggtcgagtg accctacctc ctcatgtatc tggttcagaa
      661 ccgccttgat gcgcgggccc ttctggttgg ccaaaccggc aacgcgtacc gcggcaatga
      721 tctcctcgtc tggggcggta cgcaccgcgt cccaatcggc aaaggtctga cgcagggtat
      781 tgaacgcccg atcgcgatta ctatcattcg tgttctgcga aagaatcgtg gagacaagct
      841 catcgatcgc tggcaaagga tcacgccaaa ccggcgcgcc gtaatggcgt ttgagcacct
      901 gaagcacaga gcggatttgt tctgctggcg atgtaggcgg catgtgattg atgttttgcc
      961 tttaccttat aacacggcag gttgttctgg atggctgccc gtactgtgct tgagatactg
     1021 caccaggccc cagcgcgcgc gttgcaggtg ctgctgctct tcttcctttg gcacattgca
     1081 ctgtttgaga atgttggcgg cggttttctg gatgtcttca ttgcgcaggg gttttccgcc
     1141 cagcttctcg agctgcttgt tgatcttttc cagtattttt aactgctctt ttatatcatt
     1201 ggtgctgatc agcccgccgc gacccgagac aaggatataa gagcggtaaa ccggtttcag
     1261 cagcaaagag agtgtatccc tccattgcgc caggtcggcg ttggccagga agggcacgga
     1321 gtggggcgta acagtatcac cgataaaaca caccttttcc tctggcaggg ttacccaaat
     1381 tgccgccggc gaatgaccgg ggtgactgtc aagaagcaag gtatgcgcat cagagtgtat
     1441 ttcgagatgt tcggtgaagg taatttcagg cacgctccaa cggatattgc ccagcgtgtt
     1501 gtgctgctcc caggcggcgc ctgtttcaat cccctgtggt ttgatattga ccggccggtc
     1561 tttgaatagc tgggtgagtt tttcatggcc gatgacagga ccttcgatct gccttgagcc
     1621 taatgtgcgg tcgtagtggt catccaggct gatgagcaga cggtctgtgt taggcgccaa
     1681 ttcatgcatc gatacccgcc ataaccggat atcctctggt ctgaagggcg cgtcaataag
     1741 gatcaggctg cgcttccaat tgatcaatcc cagcgtaacc cctgggtact ccctttcaat
     1801 gtaaatttga ggagcgattt cctgcatctt ttatccttcc ggagtaagct cctgccgttg
     1861 actaagcggc aggctgaacg cgaacctggc ccccccggcg tggaggttct ccacccagat
     1921 tttaccaccg tgcgcctgta cagcgaggcg gcagaaagcc aaccccaaac cgatccccct
     1981 gactgcgctt tcatcatcaa tcctggcgaa tttttcaaac acccgttctt tggcttcttc
     2041 aggaatacca gtaccctcgt cctcgataaa aaccacgacc tggtgcgcct gcgcttgaat
     2101 cctggttgtg attttactgc cgggcggtga ataacgcgcc gcgttttcca acagattaat
     2161 gataacccgc cggatcatgt cctcatccgc gttcaccact ggaaccttgc gcggcacctg
     2221 cttgaccact tcaagctttt tgctgcgcac cttgggtgag ataacctcga tgctttcatc
     2281 cagtaatttt ttcagctttg aacggcgaag ccgggtgagc tcttgacctg attcaagccg
     2341 gttaatatcc agcagcgaac tgaccaggcg ctgcatatgc gcgctggagc gcccggcaat
     2401 ctgcagcaat tgttgcgcgt ctggaccggc ttcagggggg atggtttctg ccagcaattc
     2461 cagccctgaa atgatgttgg cgagcgggga acgtaaatcg tgatagatca tcgcgctcaa
     2521 attgtcacgc agggcgtcaa gctcatgccg cgcactggtg tcgcggtata tccactgcag
     2581 acagtcaagg tcgcggtaag gcacacgcac ggcgctaacc tctaccggta aacgctgctg
     2641 gcctttgatt gacaggcttg attcataatc cccagcgctg gaacgcttgc ggcctgcccc
     2701 agcgcgggtc aacacgtcac ttgcaagagg gtccaggtgg atgaccgagc ttcttaataa
     2761 aaagcgccgg tcaaagccgc taacgaaaat gccctggcgg ttacagtcaa tgatctgacc
     2821 gtccagggtg gtgataaaaa ttggatccgg gctgccttcg aacaaaccac gatataaatt
     2881 gcgactgtgt ttggcgtctt cgtataaccg cgcgttgtga atggcgacgc ccgccaagtc
     2941 ggcgatggcc tgttgcagct tcagatgggc ttcattaaag ttccccggca aagggtggac
     3001 gatggtgagc acacccacaa ttttctcacg ggcgtgcacc ggcaggcata aagcggattt
     3061 tggcgaacct gcggtttctt tttcataggg tctttcgagc cagcgcgcgt cagcacgggt
     3121 gttttcaagg atcacgggct ggcggttgtg aatgacccaa ccagccaaac catagcgcac
     3181 gatcgcgttg atggtttcgt gtgaagcgga tgaaagctcg cctttataaa ggatgaccgc
     3241 gtcacacggt ttgtgggcat tgtcaagcac gatcaggctg gctctttccg cgccgaggtt
     3301 gctggtggaa aggagcagca cgcgcgccag cacttcgtgc aggtcaagac tggctgccag
     3361 ttgacggctg acgttgtaaa gtatttccag ttgtgcgtga tcttcagtga tcgacatggt
     3421 cttgctggaa gtatttaagt ccggttcctg tgttaaataa tacgatgctt tccccatatt
     3481 cgcaccagcc ttttttaagt aaaattttta aagccgccag cgttgccgcg ccttcaggtg
     3541 cagccaggat gccctcagca cgcgccagct cgtgctgggc gagggtgagc tcttcatcgc
     3601 tcacgctgac agcgcagcct ccgctctggt aaaggcagcg cagcaccagg cggtcggcga
     3661 agaccgcagg tacgcgcaac ccagccgcga tagtagcagc gttctgccag ggttggatgc
     3721 gctgcgcgcc ttcttcaaac gcccgaacca ccggagcgca acccgctgcc tgcaccgaga
     3781 ccattcgcgg tctggcgctg tcaatccagc cgagagattg catttcatcg aatgctttcc
     3841 acatcccaac caggccggta ccgccgccag tgggatagat aatgacgtct ggcaacctcc
     3901 agcccagcgc ttcggcgagc tcgtagccca tgatcttctt gccttcaacg cggtacggct
     3961 ctttgaaagt ggaaacgtca aaccagccgt gagcgcgtga ctcggcggca gccaggcgcg
     4021 cggcatcatt gatgagcccg tccaccaggt gcacatccgc gcctgacatg cggatttcgt
     4081 actggatggg tgccggggtg tcttttggca tgaacacatg cgcggcgcag ccggcgcgcc
     4141 cggcataaag ccccagcgcc cctcccgcgt tgccggcagt tgggataacg aattcacgga
     4201 tacccagctc cagcgctttc gaaaccgccg ccgccatgcc gcgcgacttg aaggtaccgg
     4261 tggggttgag cgactcgtcc ttcagcagca ggttggaggc acccaaagcc gcggcaatgg
     4321 tgggcagagg caataaaggc gtatcgcctt cgccaagtga aacgcggttg tgcgggtctt
     4381 ccactggcag caactcgtgc cagcgccaca tgccgcgcgg tcgggcgctg atctcctcac
     4441 ggcgcagttc cgctttcagc cgctcgagcg ggtaatgcga aagcaagggc gaattgcaat
     4501 cgtggcagta agtttgcggc aagagggggt caaacgcgcg tccgcattcc gggcaagaca
     4561 aagtgaagcg ttgtgaggga gatggattca taaaatgagt atagcataag gggcgtttcg
     4621 gatgaaaggg tagcaccaaa acgcttcaga aggctctctg aatgccggga ggtaaatcct
     4681 tttttgctca gggggtgcac accgccgggg tggtcgcacc ggctttattg ttcaatgcca
     4741 ttcccagata gggcgaaggg tcaagagtgt tgtcaatttc cagttcattc agaaagcggc
     4801 cgttaccgtc gtctttcacg attgaaaaat gcaggtgcac gccagtgggc ttgtcgcgtg
     4861 tgcccgagta gttcccctgg tagcccagca gggttccggc cttcacaaag acttcgctgc
     4921 tgccgggcgg aaattgcgcg gcaatcaggc tttcacctga tgggtaagcc atatgagtgt
     4981 aatacgtcca gatctgccgc tgtggttgca gcgggtcagc ggggatgcgg atgatcacag
     5041 tcgatttcca gtcggctaac cgggtgaggt aaccatcgta agcggcgtaa acaggcacgc
     5101 tgccgggctc accaccgccg aaaatatcaa tgccctggtg atggtgcccc gggcgaaagg
     5161 agtcattcca taaataaccg atataaccgc tggtcggcag gctgaagagg gcgccgtcgc
     5221 agcgttgacc ggcttctata cgccaatcgg gatgagcggc ggggtcgcgc agccacttca
     5281 tcaggtcacg cgagcgcggg ttaaaaaggg aggtgttgac ccataaaaaa tacactgcca
     5341 caatgacagc gatgcacaca cccgaaccca aaaggaaaaa gaagcgtttc atacgctcag
     5401 ctgacgagtt ctcccagcat agaccagggc ccggtgcgga tgatcttcac cggcagtacc
     5461 atgccgcgca ggttcgtttc agtttctacg aagaccagct tattggtagg tgtgcgccca
     5521 cgccagcgtt tcttcacctt gtcttcgaag agcacctcta cggtggtgtg aaggtaggtc
     5581 gcgtggatct cgcgcacgat ggcttcctgc agccgctcca acgcccggaa gcggcgccac
     5641 ttttcagtct cgggcacgtt atcttccagc gagcgcgccg ataaagtgcc agcgcgcggt
     5701 gagtaacggg ccaggtgaac gacatccagt ttcaattctg ccagcaagtc ataggtgttt
     5761 tggaactgtg cttcggtttc accggggaaa cccacaatga tgtcagtggc aatggaaacc
     5821 ccgggaatgc ggtcgcgtat tttctgcacc aggcggcggt aatcttccgc ggtgtagccg
     5881 cgcttcatcg cttgcagaac gtcatcatcc cctgactggt tgggaacttc aatgtgcggc
     5941 ataactttgg gtagttctgc cacgcactcc agcagctcat ccgtcatcca gttggggtga
     6001 gaggtaagaa aacgcagcct ttcgatgcct tcaatgccgc tcagctcgcg cagcagcccg
     6061 gcgagcgtgg ggtaggccgg ctggtctttt ccgtaacggt ccacgatctg ccccagcaag
     6121 gtgatttcct taacgccctg agcggcaagc gagcgggctt cacggtagat ctcttccggc
     6181 ggacggctca cctccacacc ccggcggtac gggatgatac agtaggcaca ggcgtgcgaa
     6241 cagccgtaaa caaccgggat ccaaccggct acctgcccgc cgctgctacg cggcagcacc
     6301 aggtctccgt ccatcaaggc gaagcgcctg gcggtctcgg cttcttctgc actgcgggcg
     6361 tcgcgttgga taaggtattc gaccaatggg gcagggtctg atgggggaga aaaaacgtcg
     6421 acatagggga agcgctggcg cagtggttgg tggtctttta tcccgaccag gcagcccatc
     6481 aagttaatta ccacttccgg gcggtcgcgt ttgatcggca gcagagaggt gagcctgccg
     6541 tacgccttat cttcagccga ctggcgcacc acgcaggtat tgagcacaat cacatccgca
     6601 tcttcagcgt tggaagtgtg tgtgtagccc agatgttcca gcgctgaaga aacacgctcc
     6661 gagtcagcca cgttcatctg gcagccttca gtccaaacat gatatttcat aggcgccatt
     6721 ataacaaaag aagaggttcg ctcgccgggt caccttttta cgaactgtga cgaggttctt
     6781 tagggtaaaa tgggtgaaat atctcacagc cctctcttgt ggatgggaca gatgaaaggc
     6841 gatggctatc aatcatgaat aaaaaaatcg gagtgacttt actggtgctg attatttcat
     6901 cctgcgtgct cttgagcgcc ggagtgatca ttggcgcgta tcttttgtta aaagcgcaga
     6961 aaaactatac cgacccccta cttactttga gaaacacgcc agcagtggcc acggtcactg
     7021 cagcgcagca aacacaggcg gcagctccgt ctgatggact tccgcccaaa atcgccaaac
     7081 aaatggatga gattcagcag caggtgatcg ccatccgcgg gctccacatg accaccgact
     7141 tgaagcgaga cctgctgacc ccggaagaat tacaggataa agtgatcaac gatttcttcg
     7201 ccgattatag cgatgaagac gcgttgaaag actccaaagt actctccaca ctggggttgc
     7261 tcgatgaagg gtttgacctg aaacagtttt acctggacct gtattcagaa cagatcgctg
     7321 gttattacga cagcgaaacc aaagacatgt acgtcattgc tggagaaggt tttggcggta
     7381 tggagcgcat gacttatgcg cacgagttca accacgtgct tcaggatcag aactatgacc
     7441 tggagaacgg tatgaaactg aatgatgact actgcaaagt agataccgaa tactgcgccg
     7501 cggtgagcgc gctgattgaa ggtgactcgg tttccactga gaatgaatgg tttctgaagc
     7561 acagcacccg gcaggatcaa aaggacctct tctctttcca ggaagaatac accagcccag
     7621 tctacgactc agcgccggcg tacatgaaag aagatttctt gttcccgtac acctttgggc
     7681 ttgactttgt gcagcagttg atcaaccagg gtgggtgggc ggccgttgat gcggcttatg
     7741 caaaccctcc tgtttctact gaacagatcc tgcatcctga aaaatacccg gatgatcgac
     7801 ctgttccggt ggaaatgcct gaccttgccg gtatgctggg tgatgactgg agcgaacttg
     7861 accgcaacgt gatgggagaa tggtacacct acctcgtatt tgccaaaggg aggtcctccc
     7921 agttctccat ggatgatgaa aagagcaaaa cagccgcagc aggttggggt ggagacacct
     7981 atgtttatta cgccacagca gacctcaagg aacacctctt tgcctggcgc agtacctggg
     8041 acacctccag tgatgccggc gaatattttg atatgtcacg cgactatggg ttggcgcgtt
     8101 ggggcatccc ggtacagaac agcaacggtg ttatctcctg gaactcgacc tctgacggca
     8161 taatcaccat gcgccgcagc ggtccggatg tattgtgggt gataggctcc tcaaaagaag
     8221 tggtcgatga tgcactgacg gaaattaaag attttggaaa ataagcatgc cgcttgacct
     8281 tcagcgcatt aaagggctgt gctttgatgt ggatggcacg atctgtgata ccgatgatgc
     8341 ctgggtcgag aaaataaccg atttctttca accggttgcg aaaatcgtta aatgccagaa
     8401 accgcggcag tttgcacgct ggctggtgat ggcggctgaa tcaccgatga acctggtgat
     8461 gtactggctg gataccctga gcctggatga taacttcgcg cgcttgttta cgcggatggt
     8521 gaaaaggcgc gaatataaaa acacggcatt cagactgatg aggggagcga atgaactact
     8581 gacaatggcg gagaaaaaat acccgcttgc tgttgtcagc gctcgtgatg aaaaaacgac
     8641 catgatgttc ctcgagcaat tctcgttgag caaacacttt gtagaagtgg tcacctctca
     8701 aacatgtgaa cgcaccaagc catacccgat gccggtgcag cacgcagcca atgccatggg
     8761 gttgcaagcg cgtgactgcg tgatgattgg cgataccacc gtagatatcc ttgcgggcag
     8821 gcgcgccggc gcgcaaacgg taggcctgtt atgcggtttt ggcacagaaa aagaattaaa
     8881 aaaagctgga gcggatgttg tgctgaacga ccttgaagaa cttcaagcct tattgttccc
     8941 ggttggtgga aattaaggag caactgaaaa agagatcttt tcgctctgga tggagttccc
     9001 ggcgacatcc caggctttca gatacacact gtgtttcccc gcagattcgc tcaactggta
     9061 aaggtagggc gctgctgttt gttctgacac tcgtttgcca tccacccacc attccactct
     9121 ggctactcca accaggtctt caacctgcgc ggtgatggtc acctcaccgc gtacgggcgt
     9181 gatgtgctgg tcaggcaggg gataggtgat acgcgcagaa gggggggtgt tgtctactgt
     9241 cacctgggtg accgcggagc gaaccgcgtt gtcggcatca atgaccgtca ggcggatggc
     9301 gtacagcccg ttcaacccgc tggtatccca ttcggcgagc acgccgtctt cgctcaggct
     9361 gcgggttgat tccttaactc gcagccagct ttcaggatta atcccttcgc caacctgaat
     9421 ggaataagaa gcgaaatggg ctgccgctgc tgttccacgg atgaccactt tccctgctac
     9481 cgctgaaaac atcagcggtt gagtgatgtt gaccagctca tcttgcctga tggtttgaat
     9541 agcatcatac ccctgtggga ttagttcaat cccggcgctt tgcgcccaga ggcgcgcttc
     9601 tgctgggaca ttcataaaca ctttttcatc gatcagttcg ggaggtgtaa acacggttgc
     9661 cagctgaccg gtttcgcggt taaccttgaa ccgggtgaag agcgtgtctg gcagagtggg
     9721 ttcgttcccg taaaggaaat agtcatagac gaggttgggg cagtccatgg tcggcagcat
     9781 gccagatggg ctgcatacct gaagctgcgt gatgccagcc gggcgctgcc agcctttatg
     9841 ctccagcccg cgagaagcat acttaatcac cgcgtgccag ataccggctg ccatccgctt
     9901 atctaatcca ggggtatcct tttcagttga acgggaaccc atccatacca gtaccaggcg
     9961 ctgcggggta tagcctaccg tccaaacctg gtgcccgtta tccgcgcgtc ccattttggc
    10021 cgcggcttcc ataccaattt caagttcgtt ggggtgcccc aaacttggcc agcgcgcgct
    10081 ttcatcgctg agtacatggt tgaccagata agagacgctt tcactgataa tgacggcgtt
    10141 ctcagccaat ggcgcctgcc agatggtttg atggcggctg gtattgaccc ttaacaccgt
    10201 caccggtttt atgcggttgg tcgccttatc cagccggccg ctgcgcacgc ccgcgtttgc
    10261 cagcgtgctg taagcggagc ccacttcgag caggctcaca gggctgccgc cgtacaacgg
    10321 ggaagcgtta tcaggcgggt tttcccacga atccagacca atgtaggcag cagagcgcca
    10381 ggtgttggcc gcccctgttt cattgacaat tttcgcgagg ggggtcaggt aatcgttggc
    10441 cagggctgaa cgcatgttca ccgcgccatg nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10501 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
    10561 nnnnnnnnnn cacctccagg ccgccgcgcc tgagccgctg ggcggggagg atggattcca
    10621 gctccttctc cacgattttt acgaaaccgt tttcggccac ttcaacaggc ttttttgctg
    10681 cgcgcaggtg aattacctcg ttcagcgcct gttcatactc ttccgggctg atgacgttaa
    10741 cggcgcgcat actgcgcaat agctcttttt gtgaattgaa ggcggcttca ggcgcatcaa
    10801 acggggttaa cgccgggctt ttcaataacg aaattaacag cgccgattca gccagggtga
    10861 cctgttgagc ggacttaccc aagtacaggc gcgacgcact ctccacgccg tatgcgttac
    10921 gaccgaacca ggcgctgttc aggtaccatt caagagccct ggttctacca taggtggaga
    10981 cgacctgccc ggcgaggata cgcattcgaa cagcgcgggt gatagagggc ggttcatccc
    11041 aaagaatgag ctcgctcacc aacctctctg cgatggtcag gggctcaaca gtgtgccact
    11101 ctttcaagag atatcccggg ttgctccaga aggttggatc tatcgcagtg atggcgctgc
    11161 gcaccaattg agggctgatg aattctggat tatcagggtt gaccggtaaa aactttcttt
    11221 caatgccttc atttgaatag gcaaaaaggg taaccacgcc ggaacggtca tacaacctgg
    11281 tgggctggag aagctcgcca ttctcgcggt ccatcaagct gggcaggatt tcgatcgagg
    11341 gcaagtcgcg cgcgacgagc gcgtaaaaat accccgctat cagtagtgct gcaacgatca
    11401 ccagtgaaac cacacccgcc agggcgcgtg ccaaccaggt aaacgcgcgg ctcgcccggg
    11461 ccgtgtggcg ttgccgtgaa tgcgaacggt gtttgaggag caggtaaagc gacgtcttgc
    11521 gcacgggttt ttaccttcgc ggggtgggag aacttgtttg cgcgctgctg cctgctgggc
    11581 tgggggttgg tgtacgggtg acggagaaag tggcgctctg cgttggtgca ggtgtggcgc
    11641 tggctgaggg agtgcgcgtt gtggtgcggg tttgactggt tgtgggtgtg gatgtagcgg
    11701 cagataattg atgaagcagt ttccgggctt cttcacgcag gtctgccgga aggttttcca
    11761 cgggcaattc cagcaaggct ttcaagtcag ccctggccga atcgttttgt tttttcgccg
    11821 cgtaggcgcg tgcccgccaa tagagcgcgg tggctttttg ccgatcattc ttggccagct
    11881 cgtaggctcg gcgggaaaat tcaatggcgc tatcatttaa tttccgggtc aactgcacct
    11941 gcgatttgag gatattggct tcgaatgaag tctcttgaat ctcgatggcg ctgtccagcg
    12001 tttcagcggc ttcaagataa gcgccttgct caaacaagac gcgtccacgg gcaatgagcg
    12061 ccggcacgga gcgttgatcg atgtcgaggg cgcgctctgc atagagaaaa gcctcctgca
    12121 ggttgcccgc ttcgacgagc gccagcgaca tcatcgacag ggcttcggcg tcattggttt
    12181 cgtaggtcag gtaggtttcc agcggttcaa tcgagcctgc gacctgctcg ttgttttgca
    12241 tggcggcggc gatgagctta tacgcctcga gcagcgtgat gtccaaacgg ttggcgtgct
    12301 gcgcggcgga gagcgcttct ttagcgttgc cgctgatcag gtaggcgcgg gcgcgcagca
    12361 attccacctg ggcgctgaga gagaatcgtt cttcatactg gcttaagctg ttgagcgcgg
    12421 caacagcatc accctctgat aactgcagtt cagccagcgc ggacagggct tcagagagcc
    12481 ccgggtcaag ttcgagcgct ttttgtaaat attgggcagc gtgcttatga tcaggtgatg
    12541 cggaagacat agaaacacgc gctttcccca ggtaggcggg cgcgaaagcc acgttactct
    12601 tgaggctgtt atcatatgcg gtgtccgccg cttttaacat cccttgaaag cggtaaacct
    12661 cgccgatgtg ataccagata tctgctgaat cgggttcagc gtccaccacc tgctggaaaa
    12721 acaccagggc gttgacccaa tctccttttg tgtaggcgcg catggctgcc atatacgctt
    12781 cagagagttt gtgcggagtg gccgcgtaga taggcgtggg cgtgtaggtg gcctccaaca
    12841 gcgtccataa agggggagcg ccggttgtaa acggggttgg cgtgctcgtt agcgtggcag
    12901 aataaggcgg cgttaaggaa aacttcagaa tgggcgaggt atcgggccgg taacggttgg
    12961 attctaccag gaagaagacc aaagcggaga tgctgaaaac cagcgcgccc agcatcaccc
    13021 aggaggaggt tcgcggcttg cgtttcaacg ttttttgctc gacctctttt tcaatttcca
    13081 agctggtttt ccaatcgcgc ttcagcggat cgaacccagg gggcatctcg gggggctcaa
    13141 tggcatatcc cagtatacgc agcccgtgaa tggcggtggg gtttttacgg tcaagcttga
    13201 gcacctctcg caggcaaaat tcccgctctt tgagggtttc taccacggcg ctcatccaca
    13261 gccagtattg ggggttagag cggtcgaatt gcagcaaccg caccaggtgt tcgcgcgcgg
    13321 cagcgttctc cccctgttca atggcatctt tcgccatttt gaacaactgc tcttctgatt
    13381 tcatggcatg agtgtagcat atggctaatt gcagagcaag cgtacaactg cgttataatc
    13441 aaaagagggc atttccccgc agcttgctgc gaaaaatagt aagattaa
//
LOCUS       DAOTLW010000045        12642 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_5410_length_12642_cov_9.652101, whole genome shotgun sequence.
ACCESSION   DAOTLW010000045 DAOTLW010000000
VERSION     DAOTLW010000045.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12642)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12642
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5410_length_12642_cov_9.652101"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..935
                     /locus_tag="P5516_07230"
     CDS             <1..935
                     /locus_tag="P5516_07230"
                     /inference="COORDINATES: protein motif:HMM:NF019552.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HRT92263.1"
                     /translation="GTLLLPFGCDEFRDYRPTAQAAAIAFLGQPCLPAGHWDELANWL
                     GLTVKPTQPSLDFHSSPAVRRVGKGTGWGTLRGVRFRSRPAHADQLHVDLWWDGHNIA
                     RDAGTYSYNLPPPWQNALDTTSVHNTITVNHQDQMQRMSRFLWLEQAQARWLEHNQEG
                     YITANHNGYRRFGITHQRTLQSIHGSGFLVTDRLLKKGENVQLVEYCLHWLLPDWNWQ
                     LKDQTLVLHGQPFTLRLIINARQMAPIEALEPKDVSLVRAGHTLVGTRADIRLGWESD
                     TYSEKHPALSFSLVYNSTRSIELITRWEISDAER"
     gene            922..2178
                     /locus_tag="P5516_07235"
     CDS             922..2178
                     /locus_tag="P5516_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT92264.1"
                     /translation="MQNDKQEKISSMHILLIHQAFAALDEPGGTRHYEMAKFFAEKGH
                     RVTIITSPVSYLTGKASQARQRWVTRENPEPGIEILRAYTYPALHRSFFHRLISFVSF
                     MFSSFCIGLGVKNVDVVWGTSPPIFQGWTAWLLARLKGRKFLFEVRDLWPAFAVAMKV
                     IRNPLIIRLSNWLERFLYHHADTLVVNSPGFIPHITSRGGSNPVLIPNGVDPAMFDPE
                     DNGSEFRQQLKLEHAFIVLYSGAHGLSNDLGTVLKVAQLTAQDSRIVYVLVGDGKEKA
                     ALQQLAHDLGLQNVIFVPSVAKTEMKKVLAAADACLAILKPIELYKTTYPNKVFDYLA
                     AGRPVLCAIDGVIREVIEEAHAGIFVPPGDPAALADAVIRLSSAPDKGKKMGLNGRAY
                     VVEHFNRRDIAARMEKVLFECANNPR"
     gene            2286..3335
                     /locus_tag="P5516_07240"
     CDS             2286..3335
                     /locus_tag="P5516_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92265.1"
                     /translation="MASTQLTPAEQLNRDIASTRSEITRLVDEVKLTSVRNTYSTLDS
                     AIANLPLRVQKVRARKYAFNKILETQAEEFQKQWALKRSHVLNQITTESNALQSQLRV
                     LEGRAAGIANSAAVKMLQSDITNFKARVSASKRAVTDLFDKLNNEVNKVQSQMSQIEK
                     TLDLAESACFGFLPTESVVRAVKALWTRDEQEDKTDPKGILFLTDQRLLFEQREEIAT
                     KKVLFVTTERKKVQELLFEVPVFSITSVQATKQGMLKNEDWLTLEFESGAFARGAKIH
                     LDGQNCDEWQKLITQVKSRELDSDRAIEIDSQAVEKAKAAPVICPFCGGSITKPVLRG
                     MDSIKCDFCGNVIKL"
     gene            complement(3631..4509)
                     /locus_tag="P5516_07245"
     CDS             complement(3631..4509)
                     /locus_tag="P5516_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRT92266.1"
                     /translation="MTEKKVLLAVLAHPDDETFGTGGSLAHYARAGVEVHLVCATRGE
                     VGEVDPLLMGEFKSIGELRENELRCAAGYLGLAGVHFLNYRDSGMAGSPENHHPQSLV
                     AADQQKVIGEIVHYIRLLKPQVVITFDPIGGYMHPDHIAIHQAADKAFYAAADPALFP
                     SNLPAYQPQKLYYHTFPRGFLRTTVWLMKIAGKDPSRFGKNKDIDLLKISQARFPIHA
                     VVDYRDVIDQKHQAVACHASQGGMRITGGVLDKLRKVFGVSDQYMRAYPPPTRRRERD
                     LFDGVILTDAASENRL"
     gene            complement(4539..5315)
                     /gene="moeB"
                     /locus_tag="P5516_07250"
     CDS             complement(4539..5315)
                     /gene="moeB"
                     /locus_tag="P5516_07250"
                     /inference="COORDINATES: protein motif:HMM:NF004281.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HRT92267.1"
                     /translation="MELQLCSMELSDLERQRYKKHLQLESIGVEGQLKLKHGRVLVIG
                     AGGLGSPVLIYLAAAGVGNLSILDDDLVTLSNLPRQVIHITNKIGTPKVNSAWERLEQ
                     MNPEISFRPIQERLQPANAESIISGHQVVVDCTDNLDARLLINETCVKASIPFVYGAV
                     FEYEGQVSVFDAKRGPCLRCMLPEQLSAGTIPNPENHGLLSTVPGVIGLLQAAEVIKL
                     LTGIGDTLVGVLALYDALAVNLTKIRIAKRENCPVCGTAA"
     gene            5377..8022
                     /locus_tag="P5516_07255"
     CDS             5377..8022
                     /locus_tag="P5516_07255"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRT92268.1"
                     /translation="MNVVLTHEQADFDALAALLCAHLLNEHAIPVLPNRLNRNVRHFL
                     NLYGDKFPFVEARDLGADSIQQVTLVDTQSLVTLKGINTKTAVNIVDHHDPRTDLPAQ
                     WHTRFEKVGATTTMLVDLLREHARPISIIEATLLLLGIYEDTGSLTYASTTVRDVNAA
                     AYLMEKGASLKIAAEFLNPALSEGQKKLADRLLENAQTINIHGKTIILSCANAVDFNE
                     EIASIAHKLRDLLDPDALFLFVRTVEGIRLVARSTSDQVDVARIAQHFSGGGHERAAA
                     ALIQAAEDAEEVALDVFCHEVIEELERSIEPPLTLNKIMSKKPHLISPSTSLNEAHLL
                     MQRYGYEGYPVVDNGKVVGLLTRRAVDRALSHKLNLNAGSLMEAGEHFLKNTDTIDKL
                     QQLMAATGWGQVPVVDAENGKIIGIVTRTDLLKTLPGHNGIIPGQRNHASMLEKALPA
                     SRLALLKLVAGASEKGQHAIYIVGGFVRDLLLERPSIDFDIVVEGDALQIAQALSQQY
                     GGRVIPHKRFSTAKWFIKPIRANLLKRMGIENGRATDLPEALDFISARTEFYDHPTAL
                     PTVERSSIKLDLHRRDFTINTLALRLDGRHYGELYDYWGGLQDLEKKQVRVLHSLSFV
                     DDPTRLLRAVRFEQRFHFQIEKRTLQLMTEALELLHQLSGERIHHELDLIFNDHDPVS
                     IMRRLEELGLLRHIHPGLRWQGQNDAFFAQAINYAQFSDWNLPDLIGNTTTRHALIYL
                     TWLASLDGNTGLEVANRLRLPRQLQDTLAHIISARQQLDQLAAGLPSQLTLELEKIPL
                     YGVFALYIVCEHSSLKDSINLYATKWRFIFPGVDGNTLLARGIPPGPKYTSILTAIRD
                     AWLDGRVTNEKEEALLLERLLKEIQ"
     gene            8019..8558
                     /locus_tag="P5516_07260"
     CDS             8019..8558
                     /locus_tag="P5516_07260"
                     /inference="COORDINATES: protein motif:HMM:NF024898.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT92269.1"
                     /translation="MTDLSTDHPSRAPLDEILVRELKKKDLPALEWDGEYTHFRNVYR
                     GVYQRTRLGTAAAWVAESPVDGIVGQVFLQLDCDRPELANGWNRAYLYSFRIKPPYRG
                     LGLGTRMVKTLEAYLVARHYSRLTLNVARDNLEAIRLYHRLNFQIVGEEPGIWSYIDH
                     LGKWHTVEEPSWRMEKHLL"
     gene            complement(8562..9569)
                     /locus_tag="P5516_07265"
     CDS             complement(8562..9569)
                     /locus_tag="P5516_07265"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HRT92270.1"
                     /translation="MMKLRSSVQGALILALLVLTGCHRASDSSSLFLGGDVLLYRAGK
                     PLFPVEDEAISPWGDFIKVKQEEHADLFAVNLESPFGLRVVEHGLENPSMNLCADDDA
                     LQHLQQAGVNLITNHNNHVNDCGFSGQQYTDELLDRAGILHQRNHSSVQFVPAGGAVI
                     AVLSINDYERDYEIVTVVNELKKARGASDLVLVSVHWGQEYQAGPSQHQQELAQLLVD
                     AGADVIWGHHPHVLQRMEWLRSSADGHTGLVMYSLGNALSDQWMLPDAQRTALIRLTF
                     SHHQVKDILVVPLKMDMSSRTLLLEKDPQLFNWYVERLSLESLEKKGTTIRLFEFAQT
                     P"
     gene            complement(9566..11803)
                     /locus_tag="P5516_07270"
     CDS             complement(9566..11803)
                     /locus_tag="P5516_07270"
                     /inference="COORDINATES: protein motif:HMM:NF022812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3160 domain-containing protein"
                     /protein_id="HRT92271.1"
                     /translation="MNRLFKVLSVLLIVAFLLSACQLPFNLVERIKNALIEKGQSGEV
                     TEPAAQGTETTTTQPPVVYPAEGMAPVFAAYQQRPYTMPEVYQGGYTLPLKASELVNL
                     DLFDLTDSQVEALLNNGFVVQPPNNDPSRMYNEFYQAYESIRYEETPVFVTTDAIFHV
                     YHLIFDKMLRDLERTFFIDTLKELTTAMVTESVAQYEKLQGSPLEEAAIRNVAFFTVP
                     AMILDLPVSVPAEAKKLAEEELALINEGGGISASPIWKTEGQATDNVLLEDYSQYIPR
                     GHYTRDEALKTYFKAMMWFGRMTFRLMDPIETQRALLVVQAMRNASTTSGKPALELWQ
                     NIYDPTVFIVGKADDLSIYEYGKLSDEVFGVHPELTSFADPLLAEKFTQAVKQLPPPQ
                     INSMWVYIWQERDAVTQGFRFMGQRFTLDQYVFGKLIWRKVGTTENPRDLPKSLDFLA
                     AQGSEEAYNLLAEMGETKFENYDSQLAKVKEEVAGFGIDSWTQNLYWSWLYALQPIFA
                     PKGEQYPPFMRTQAWLRKDMQTALSSWTELKHDTILYAKQVMAEMGGGALDKPPRGYV
                     EPNLEAYARLLALTEMTRSGLENREILDDVTRGNLDNLIEELVFLLDVSQAELNGVKL
                     TEEQYWRIQYFGGWLESMTIAASDPADVALGGGNLSDQKSALVADVATGMGRVLEEGV
                     GYPTKIYVVHPEAPYEIAVGAVYTYYEFDVPLAERLTDEAWQKMLETGKAPAAPEWTG
                     MFIVK"
     gene            complement(11823..>12642)
                     /locus_tag="P5516_07275"
     CDS             complement(11823..>12642)
                     /locus_tag="P5516_07275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92272.1"
                     /translation="DNHRNGYTRYTELSKPAAQSIGVGSTIITGTLFMLKTSFHKWRL
                     LLVSLLALANSSPMFYSLWSYSGGEIHRLTGTETKLKVTSGGMQATSTNSGELCVLFQ
                     EDHVKVDGCDKDSPITPWQSPPEWEVREVIVSDLNWDNQEEIAMLVWRPFKPWPVDRF
                     MPSAGRINTFHDKNDMSCHLILIGLRNGNIKELWAGSAMADPLREIHAADLDGDGRQE
                     LVALEHAYNGNPRASSITVWAWHGFGFSLQARQNGRFSSLLVLPAEQGKVILTQ"
BASE COUNT         3026 a         3483 c         3132 g         3001 t
ORIGIN      
        1 acggtacgtt gctgttgcct ttcggttgcg atgaattccg ggattaccgc cccactgccc
       61 aggccgctgc catagcattt ctcggtcaac cctgccttcc agcgggtcac tgggatgaac
      121 tggccaactg gctgggtcta acagtaaaac ccacccaacc ttccctggat tttcactctt
      181 cgccggcagt gcgccgggtc ggaaaaggca caggctgggg gactttgcgc ggcgtgcgtt
      241 tccgcagccg tcccgcccac gccgaccagt tgcacgtcga cctctggtgg gatgggcaca
      301 acatcgcgcg cgatgccggc acctattcct ataacctgcc gccgccgtgg caaaatgcgc
      361 tggataccac cagcgtgcac aacacgataa cggtcaacca ccaagaccag atgcaaagaa
      421 tgagccgttt tttatggttg gaacaggcgc aagccagatg gttggaacac aaccaggagg
      481 gctacatcac tgcaaaccat aatggctacc gccggtttgg catcacccat cagagaaccc
      541 tgcaatccat tcacgggtca ggctttctcg tgaccgatcg tctgctaaaa aagggcgaaa
      601 atgtccaact cgtggagtac tgcctgcact ggctgttgcc ggattggaac tggcaattaa
      661 aagaccagac gcttgtctta catgggcagc cgttcacgct caggttgatc atcaacgcgc
      721 gacagatggc gcccattgaa gcgctcgagc caaaggatgt ctcgcttgtc cgcgcgggtc
      781 atacgctcgt tggcacgcgt gcggacatcc gtttgggatg ggaatcggac acttattctg
      841 aaaaacaccc ggcgctttct ttctcgctgg tctacaattc aaccaggtcg attgaactga
      901 tcacaagatg ggaaatctca gatgcagaac gataagcagg agaagataag ctcgatgcac
      961 atcctcctga tccaccaggc ttttgccgct ttggatgagc caggtggaac ccggcattac
     1021 gagatggcca aatttttcgc cgaaaaaggt catcgcgtca cgatcatcac cagtccggtg
     1081 agctacctca ccggcaaagc cagtcaggcc aggcagcgct gggttacacg ggaaaacccg
     1141 gaaccaggca tcgaaatttt gcgcgcttac acctatccag cgctgcatcg cagtttcttc
     1201 caccgcctga tcagcttcgt cagtttcatg ttttcatcct tttgtattgg tcttggcgta
     1261 aaaaatgtgg atgtcgtttg gggaacctcc cctcccattt ttcagggatg gaccgcctgg
     1321 ctgttggccc ggctgaaagg cagaaaattc cttttcgaag tgcgtgacct ctggccagcg
     1381 ttcgcggtgg cgatgaaggt cattcgcaac ccgctgatca ttcgcctgtc taactggctt
     1441 gagcgcttcc tttaccatca cgcggacaca ctggtggtca attcaccagg gtttatccct
     1501 catatcacaa gccgaggagg tagcaaccct gttctgatac ccaacggcgt tgacccagcc
     1561 atgttcgacc ctgaagacaa cggcagtgag tttcgccagc agctgaagct ggagcacgct
     1621 ttcattgtcc tttattccgg cgcgcatggg ttatctaacg acctgggcac agtgctgaag
     1681 gtagcgcagc ttactgcgca agacagccga attgtttacg tgctggtggg tgacggaaaa
     1741 gaaaaagccg ccttacaaca gctcgcccat gaccttggtt tacagaatgt catcttcgtc
     1801 ccatcagtgg cgaaaacgga aatgaaaaag gttcttgccg ctgccgatgc ctgcctggcg
     1861 atccttaaac caatcgaatt gtataaaacc acctatccca acaaggtttt tgattacctc
     1921 gctgctggtc gcccggtttt atgcgccatt gatggcgtga ttcgcgaggt gatcgaagaa
     1981 gcccacgcag gtatttttgt ccctccggga gaccctgccg cgctcgcgga cgcagtcata
     2041 aggctctcat ccgcaccaga caagggaaag aaaatggggt taaatggccg cgcttacgtg
     2101 gtggagcatt tcaaccgtcg cgatatcgcc gcgcgtatgg aaaaagttct tttcgaatgc
     2161 gccaacaatc caagataacc ccggcttgtt ttcacacctc tgtcacgacg gtccaaaaag
     2221 attggtaatc gaataaggta caatagtggg taatcatttg catctcatcc cacaggagga
     2281 atcccatggc ttcaacacag ctcacacctg ccgaacaatt aaatagagat atcgcctcca
     2341 cacgcagcga aatcaccagg cttgttgacg aggtaaaact cacctcagtc cggaatacct
     2401 attcaaccct cgacagcgcc atcgccaatc tcccgctgcg cgtgcagaaa gtacgcgctc
     2461 gtaaatacgc cttcaataag atacttgaaa cccaggcaga agagttccag aaacaatggg
     2521 cgcttaagcg cagccatgta cttaaccaga tcacgacaga atctaacgcg ctgcaaagcc
     2581 agttgcgcgt tttagaaggt cgcgctgcag gtattgccaa tagcgcggca gtgaagatgc
     2641 tccaatcaga tataacaaat tttaaagccc gtgttagcgc ttcaaaacgc gccgtcactg
     2701 acttatttga caagttgaat aacgaagtga ataaagttca gtctcagatg agccagattg
     2761 aaaagacact cgatttggcg gaatcagcct gttttggctt tttacccaca gaaagcgtgg
     2821 ttcgagccgt gaaagccctt tggactcgtg atgagcaaga agacaagacc gacccaaaag
     2881 gaatcctgtt tttaactgac caacgcttgc tctttgaaca acgcgaagaa atcgccacca
     2941 agaaagtcct tttcgtgacc accgagcgca agaaggttca ggagctgttg ttcgaagttc
     3001 ccgtgttttc catcacttcc gtgcaggcga ccaaacaggg tatgttgaag aatgaggatt
     3061 ggctcaccct cgaattcgaa tcaggcgcgt ttgcgcgcgg cgcgaaaatt caccttgacg
     3121 gtcaaaactg cgatgaatgg caaaaactga tcacgcaggt caaatcacgc gagctggata
     3181 gtgaccgcgc cattgaaata gacagccagg cggtagagaa agccaaagcc gcgccggtta
     3241 tctgcccgtt ttgcggcggc tcgattacca aacctgttct gcgcggcatg gatagcatca
     3301 agtgtgattt ctgcggcaat gtaatcaaac tctaacctcc tttctttcgc attccgctga
     3361 agcccgccgt agctgcggag gtatgtttgc tatcatttag aggtaacgga gtaagaaatc
     3421 acccgccacc gtttgggcgg tttgaacaac caaacggagt ggaatgcgct gggttagaga
     3481 acgtcccatt gttcgggtaa ccgcggtgag accgccgcgt aaccgccagg agagatctca
     3541 ccgtctgcca ttgtgtttga agcaaaatag tgagcaaccg gctcgtcgaa aggagcacat
     3601 ccgcaccgca ccttgtcttc agctcgttgt ttacagcctg ttttctgatg cggcgtcggt
     3661 caatattacg ccatcgaaca ggtcccgctc acgccggcgg gttggcggcg gataagcccg
     3721 catatactgg tcgctcacgc caaaaacctt gcgcaactta tccaaaacgc cgccggtgat
     3781 gcgcatgccc ccctggctgg cgtggcaggc aactgcctgg tgtttttggt caatcacatc
     3841 ccggtaatcc accaccgcgt ggatgggaaa ccttgcttgt gaaattttca acaggtcaat
     3901 atctttgttc ttgccaaacc ggctggggtc ctttccggca attttcatca gccagaccgt
     3961 ggtgcgcaaa aaaccgcgtg gaaaagtatg gtaatacaat ttttgtggct ggtaagccgg
     4021 caggttagat ggaaataacg ccgggtctgc agccgcgtaa aaggctttgt ctgcagcctg
     4081 gtggatggcg atatggtcag ggtgcatgta cccaccaata gggtcaaaag tgatgaccac
     4141 ctggggtttc agtagtcgga tgtaatgcac aatctcaccg atcacttttt gctggtccgc
     4201 cgcgaccaac gattgtggat gatgattctc aggcgatcca gccatcccgg aatcacgata
     4261 atttaaaaag tgaaccccag ccagccctaa atagcccgca gcgcagcgca gttcattctc
     4321 gcgcagttct ccaatacttt taaattcacc catcagcagc gggtccactt ctcccacttc
     4381 tccgcgtgtg gcgcaaacca ggtgaacctc cacccctgcc ctggcgtaat gcgccagtga
     4441 tcccccggtg ccaaacgtct catcatctgg gtgagccagt acagccagta gcactttctt
     4501 ttctgtcatt cttgctctat cctgttccat tggtttgatt acgccgccgt gccgcaaacg
     4561 gggcaattct cgcgtttcgc tatcctgatt tttgtgaggt ttaccgccag ggcatcataa
     4621 agcgccaata ccccaaccag cgtatcgcca attcctgtca gcaatttaat cacttccgcc
     4681 gcctgtaaca gtccaatcac tccggggacc gtagagagca atccatgatt ttccgggttt
     4741 ggtatggtgc ccgcagaaag ctgctctggt aacatacagc gcaggcatgg tcctcgtttt
     4801 gcatcaaaga cggaaacctg tccttcatat tcaaaaaccg cgccataaac aaagggaatg
     4861 gaggccttga cgcaggtttc attgatcagc aatcgcgcat ccaggttgtc tgtgcaatcc
     4921 acaaccactt gatgaccgct gatgattgat tcggcgtttg cgggttgcag cctctcttgg
     4981 attggtcgaa agctgatctc tgggttcatc tgctccaggc gctcccacgc agaatttacc
     5041 ttcggtgtgc caatcttgtt agtgatatga atgacttggc gcggcaggtt cgagagcgtg
     5101 accaggtcat catccagaat agataggttg cctacccccg cggctgccag ataaattaat
     5161 accggcgagc ccaatccgcc cgcgccgatc accagcaccc gtccgtgttt aagctttaac
     5221 tgaccttcaa ccccaatcga ctccagctgg aggtgttttt tataacgctg tctttccagg
     5281 tcactcagtt ccatactgca caattgtaat tccattctat aattttctga aactgctcat
     5341 cgtaacagat atgcgccttg atgttacaat caaattatga atgtggttct cacgcatgaa
     5401 caggcggatt ttgacgcgct ggcagctttg ctgtgcgcgc acctcttaaa tgaacatgcc
     5461 attcctgttc tccccaaccg cctgaaccgt aacgtgcgcc atttcctcaa cctgtatggt
     5521 gacaaatttc cctttgttga agcgcgggat ttaggcgcag atagcatcca gcaggtgacc
     5581 ctggttgata cgcaatccct ggtcaccctg aaaggcatca acaccaaaac cgcggtcaat
     5641 attgtggacc atcacgaccc caggactgac ctgcccgcgc agtggcatac ccggtttgaa
     5701 aaggtcggcg ccacaaccac gatgctcgtc gacctgttgc gcgaacacgc ccggcccatc
     5761 tccatcatcg aagccacgct gctgctgctg ggaatttatg aagataccgg gtcgttaacc
     5821 tacgcctcca ccactgtgcg tgatgtcaac gcggctgcct acctgatgga aaaaggcgcc
     5881 agcctgaaga tcgctgctga atttctgaac ccggcgctat ctgagggtca gaagaagctg
     5941 gctgaccggt tattggaaaa cgcccagacg atcaatatcc acggcaaaac catcatcctc
     6001 tcgtgcgcta acgcggttga ttttaacgag gaaattgcca gtattgccca caaattgcgc
     6061 gacctgttag accctgatgc gctcttcctt ttcgtgcgga ctgttgaagg gatcaggctg
     6121 gtggcgcgct ccacctctga ccaggtagat gtagcgcgta tcgcccagca ctttagcggt
     6181 ggggggcatg aaagagccgc agcggcgctc atccaggcgg cagaggatgc cgaagaggtc
     6241 gccttggatg ttttctgcca cgaggttatc gaggaacttg aacgctccat tgaaccaccc
     6301 ctgactctca ataaaatcat gtccaaaaag ccgcacctca tcagtccttc aacttcattg
     6361 aatgaagccc atttattaat gcagcgttat ggatacgaag gttatcctgt cgttgataat
     6421 ggcaaagtgg tggggttgct caccagaaga gcggttgacc gcgctttgag ccataaattg
     6481 aatcttaacg ccggaagcct gatggaagcc ggggagcatt ttctaaaaaa caccgatacg
     6541 atcgataaac tacagcaatt aatggcggct acaggttggg ggcaggtgcc agtggtagat
     6601 gccgaaaacg gcaaaatcat cggcattgtt acccgcaccg acctgctcaa aaccctcccc
     6661 gggcataacg gtatcatccc cgggcagaga aatcacgcgt ccatgctgga aaaagccctg
     6721 ccggcatcca ggttggcttt actgaaactt gtagctggcg cttcagaaaa ggggcaacac
     6781 gccatttata tcgttggcgg atttgtccgc gacctgcttt tagaacgccc ttcgattgat
     6841 tttgacatcg tcgtggaagg cgatgctctg cagatcgcgc aggcgctttc ccaacaatat
     6901 ggcggtcgcg tgatcccgca taaacgcttc agtaccgcca agtggtttat taagccaatt
     6961 cgagcaaacc tcctcaaacg aatgggtata gaaaatggtc gcgccaccga cctccccgaa
     7021 gccttggatt ttatcagcgc gcgcaccgag ttctacgacc accccaccgc cctgcctacc
     7081 gtcgagcggt ctagcatcaa gctggatttg catcgcagag actttacaat aaataccctg
     7141 gcgcttcgat tggatggtcg tcattacggt gaactttatg attactgggg aggtctccag
     7201 gacctggaaa agaaacaggt cagggtgctg cattccctct cgttcgtaga tgatccaacc
     7261 cgcctgctgc gggcggtgcg atttgagcag cgtttccatt ttcaaattga aaaacgaact
     7321 ctacagttga tgactgaagc gcttgaactg ctgcatcaat tgagcggcga acgtattcat
     7381 cacgaattag acctgatttt taatgatcac gacccggtat ccattatgcg tcggcttgaa
     7441 gaactgggcc tgctgcgcca catccaccct ggcttgcgct ggcaggggca aaatgacgct
     7501 tttttcgctc aggcaatcaa ctacgcccaa ttttcagatt ggaatctacc tgacctcatc
     7561 ggcaacacaa ccacccgcca cgcgctcatt tatctcacct ggctggcaag cctcgacgga
     7621 aacacagggc ttgaagttgc aaacaggttg cgcctgccgc gccagttgca ggatacgctg
     7681 gcgcatatca tcagcgcgcg gcagcagctg gaccagttgg ctgcagggtt acccagccag
     7741 ctaacgcttg aactggaaaa aatacccctt tatggagttt tcgctcttta catcgtgtgt
     7801 gagcattctt cactaaaaga tagcatcaac ctgtatgcca cgaaatggcg gttcatcttc
     7861 cccggggttg atggcaacac cttgctggcg cgcggtattc cgcctggacc gaaatatacc
     7921 agcattctta ccgccattcg tgacgcctgg ctggatgggc gggtaactaa cgagaaggaa
     7981 gaagcattat tactggaaag gctcctcaaa gagatacaat gacggatctc tcaactgacc
     8041 acccctcccg ggcgccgctg gatgagattc tggttcgtga gctgaaaaag aaggatctgc
     8101 ccgcgttgga atgggatggc gagtacacac acttccgtaa tgtgtaccgc ggggtttacc
     8161 agcgcacccg gcttggcacc gcggcagcct gggtggcaga atcacctgtg gatggcatcg
     8221 tgggacaggt ttttttgcaa ctggattgcg accgacctga gcttgccaac ggctggaaca
     8281 gggcttatct ttattcgttc cgtattaaac cgccttaccg cgggttggga ttgggaacca
     8341 gaatggttaa gactcttgaa gcttacctgg ttgcccgtca ttattcgcgc cttactttga
     8401 atgttgcgcg tgataacctg gaggcgatcc gcttgtatca ccgcctgaat ttccaaatcg
     8461 tgggagaaga gccgggaatc tggtcttata tcgaccatct gggcaaatgg cacaccgttg
     8521 aagaaccttc atggcgcatg gagaaacacc ttctttgaag gctacggggt ttgcgcgaat
     8581 tcaaacaatc ggatggtcgt tcctttcttt tccaaagact ccagcgacag gcgttcaaca
     8641 taccagttga ataattgtgg gtctttttcc agcagaagtg ttcgcgagga catatccatc
     8701 ttcagtggaa caaccaggat gtcttttacc tggtgatgag aaaatgtcaa tcgaattaga
     8761 gccgtgcgct gtgcgtctgg cagcatccac tggtcgctta acgcgttgcc aaggctgtac
     8821 atcaccaatc ccgtgtgccc gtctgccgaa gaacgcagcc attccatacg ctgcagcaca
     8881 tgcgggtggt gcccccaaat cacatccgct ccggcatcta ccagcaactg tgccagttcc
     8941 tgctggtgtt gcgatggtcc tgcctggtac tcctgtcccc agtgcaccga aacgagcacc
     9001 aaatcgcttg ctccgcgggc ttttttcaac tcgttaacca ccgtgacaat ttcgtaatct
     9061 ctttcgtagt catttatgct caacacggcg ataaccgcgc cccccgctgg gacaaactgg
     9121 actgaactat gatttctctg gtgaagaatg ccagcccggt ccagtaattc atctgtatac
     9181 tgttgaccgg agaatccgca atcattgaca tgattgttgt gatttgtgat gaggttaacc
     9241 ccagcctgct gcaaatgctg cagcgcgtca tcatccgcgc aaaggttcat cgatggattt
     9301 tccaaaccat gctcaaccac cctcaatcca aaaggagatt caaggttgac cgcaaacagg
     9361 tctgcatgtt cctcctgttt gacctttatg aaatcccccc atggagaaat ggcttcatcc
     9421 tctaccggaa aaagaggctt ccctgcccta tagagcaaca catctccgcc caagaacagc
     9481 gaagaactgt cgctagcgcg atggcatcct gttaagacaa ggagcgccag tatcagcgct
     9541 ccttgtacgc tcgaacgcaa tttcatcatt tcacgatgaa catacctgtc cattccggag
     9601 ccgctggagc cttgcctgtc tcaagcattt tctgccaggc ttcgtctgta aggcgttcag
     9661 ccagcgggac atcgaactca taataagtgt aaactgcgcc aacagcaatt tcgtatggtg
     9721 cttcggggtg caccacgtaa atcttggttg ggtatcccac tccttcctct aacacccgac
     9781 ccatcccggt cgccacatcc gctaccaggg cagacttctg gtccgaaagg ttgccgcccc
     9841 ccaacgcaac atcagcaggg tctgaagcag cgatggtcat ggattctaac cagccgccaa
     9901 aatattggat gcgccaatat tgctcttctg tcagcttcac cccattcagc tcggcctgtg
     9961 aaacgtccag caagaagacc agttcttcga tcaggttatc cagattcccg cgggtcacat
    10021 catccaaaat ctcccggttt tccaggccgc tgcgcgtcat ttcggttaac gccagcaagc
    10081 gcgcgtaagc ctcgaggttg ggttccacgt accccctggg tggcttgtcc aatgcccctc
    10141 cgcccatctc agccatcacc tgtttggcat agaggatggt atcgtgcttc aattcagtcc
    10201 acgaactgag cgcggtctgc atatccttgc gcagccaggc ctgggtgcgc ataaatggcg
    10261 ggtattgctc gcctttgggg gcaaagattg gctgcagcgc atacagccat gaccagtaaa
    10321 ggttctgcgt ccaggaatcg ataccaaacc cggccacctc ttctttcact ttggctagct
    10381 gcgaatcgta gttctcaaat ttcgtttcac ccatttcggc aagcagattg taggcttctt
    10441 ctgaaccctg cgcagcgagg aaatccaggc ttttaggcag gtcgcgcggg ttttccgtcg
    10501 tgcccacctt tcgccagatc aattttccaa acacatattg gtccagcgta aagcgctgcc
    10561 ccataaagcg gaatccctgg gtgactgcgt cgcgttcctg ccagatgtag acccacatgg
    10621 aattaatctg cggaggaggt agctgcttca ctgcctgggt gaacttctct gccagtaagg
    10681 ggtctgcaaa agaggtcagc tctgggtgaa caccaaagac ctcatcggat agctttccat
    10741 actcgtagat tgataggtca tccgcttttc ccacgataaa aacagtgggg tcgtaaatgt
    10801 tttgccagag ctcaagcgct ggtttgcctg aagttgtaga cgcgttacgc atggcctgca
    10861 ccaccagcag tgcgcgttgc gtttcaatcg ggtccatcag gcgaaaggtc atacgcccaa
    10921 accacatcat cgctttaaag taggttttca gcgcctcgtc gcgcgtgtaa tgcccacgcg
    10981 ggatgtactg agagtaatcc tctaaaagga cattgtcagt cgcctgtccc tctgtcttcc
    11041 aaatcggtga agctgaaatg ccaccacctt cattgatcaa cgcgagctcc tcttcggcca
    11101 attttttagc ctcagctggt acgctcaccg gcaggtcaag aatcatcgcg ggaaccgtga
    11161 agaaagccac attacgaatc gcggcttctt ccagaggact accttgcagt ttttcgtatt
    11221 gagccacaga ttcagtgacc atggcggttg tcagttcttt caaggtgtca atgaaaaaag
    11281 tgcgttccaa atcgcgcagc atcttatcaa agataagatg atatacgtga aagatagcat
    11341 ccgtggtgac aaaaaccgga gtttcctcat aacggatgga ctcatatgcc tgatagaact
    11401 cgttgtacat gcggctaggg tcattgttcg gcggctgaac gacaaaaccg ttgttgagca
    11461 gcgcctcaac ctgagagtca gtcagatcaa acaagtcaag gttgaccaac tcgctggctt
    11521 ttaaaggaag ggtgtaaccc ccctggtaaa cctcaggcat ggtatacggt cgctgttgat
    11581 aggctgcgaa cacgggagcc attccttctg ctgggtatac caccggcggc tgggtggtcg
    11641 tcgtttcagt gccctgggcg gcgggttcgg taacctcgcc cgattgccct ttctcaatca
    11701 atgcattttt aatcctttcc accaggttga atggcagctg gcaggcactg agtagaaatg
    11761 cgacgatgag caacacagag agtactttaa acaagcggtt cattttttcc tccttgaggg
    11821 tcctattgag tcaatatcac tttcccttgt tcggctggta gtacaagcag agatgaaaac
    11881 cttccattct gcctggcttg caaagaaaaa ccaaacccat gccatgccca caccgtgata
    11941 gaagatgcac gagggtttcc gttataggca tgttcaagag caacgagttc ctgccgtcca
    12001 tctccatcca ggtcggctgc gtgaatttcc cttagcgggt cagccatcgc tgacccagcc
    12061 cacaattctt taatattccc gtttcgcagc ccaatgagaa tcaggtgaca gctcatatcg
    12121 ttcttatcat gaaaggtgtt aatgcgtccc gcgcttggca taaagcggtc aactggccag
    12181 ggcttgaaag gacgccacac cagcattgcg atctcctcct ggttatccca attgaggtca
    12241 gaaacgatca cctctctcac ctcccattcc ggggggcttt gccagggggt gatggggcta
    12301 tccttatcac agccgtcaac cttcacatga tcctcttgaa aaagcacaca caactctccg
    12361 ctgttcgtgc tggttgcctg catcccaccg cttgtaactt taagtttcgt ctcagttccc
    12421 gttaatcggt gaatctctcc tccgctataa ctccataacg aataaaacat cggagagctg
    12481 tttgccaaag ccagcagaga aaccaggagt aaacgccatt tatgaaaaga cgttttcagc
    12541 ataaaaagag ttcctgtaat gattgtacta cctaccccaa tagattgagc ggctggctta
    12601 ctgagttcag tataccgtgt ataaccgttc cggtgattgt ct
//
LOCUS       DAOTLW010000046        12404 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_5529_length_12404_cov_11.107215, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000046 DAOTLW010000000
VERSION     DAOTLW010000046.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 12404)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 12404)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12404
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5529_length_12404_cov_11.107215"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1352)
                     /locus_tag="P5516_07280"
     CDS             complement(<1..1352)
                     /locus_tag="P5516_07280"
                     /inference="COORDINATES: protein motif:HMM:TIGR03025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0032508 - DNA duplex unwinding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide biosynthesis polyprenyl
                     glycosylphosphotransferase"
                     /protein_id="HRT92273.1"
                     /translation="MTQNTQNGITELALSNEYKPWLVSGLLLLSDLSAIILSFILSFY
                     LRKALIPIIGGVLDFNQIAPLFLLIILFILVIFLLFGLYPGHGRTGVVEFREIVYIVS
                     FSHIIVAMIIFILGYGPRFSRLVFLFNWCFALVLITSFRLWLHNRGSLRRWWGQPAVV
                     IGGSKDVTMVIRQLKSARRLAYRPIAAIVLDKDAPQEKIHEVPVYPFSEKQLNEFRKA
                     NIRLGIFTSYTVSTRLGFRKYLQTLSLVFPKLLYVLGDSPLNMLAMKTMDLDGHPALQ
                     VQYNLLNPFALFLKRAADLFLSLLSLVFTFPLFLLFSLIIWIDSPGPVLYKQTRMGKG
                     GKLFNIYKFRTMVVGADKKLQDLLAADENLRDEFERYHKLQDDPRVTRVGRFLRKTSF
                     DEFPQILNVIKGEMSWVGPRAYLPAELEKMGDSAAIIHRVTPGLTGWWQVMGRHSVSF
                     "
     gene            complement(1413..2492)
                     /locus_tag="P5516_07285"
     CDS             complement(1413..2492)
                     /locus_tag="P5516_07285"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT92274.1"
                     /translation="MRIAIVHDALCVSGGAERMVLWLAKAFPQAPVYTSVYLQANTFE
                     GFKALQVNTLPFSRLVKSERQFKLLYPLWYALIQREDFSNFDAVLSSSTYLAKFIRPT
                     STVRHACYLYAPFRLLWKPESYTPESLPIRGPATSLVKWVTPLLRRWDLKRTREIPKL
                     ATTCRNMANEIHKVYNMQAQVIYPPVEIPPQMAIEKGGEYYLSVSRLISHKRVDLAVK
                     ACTRLGRQLLVVGDGPERARLEQMAGPTIRFVGRVSDEEVQRLFSRARGLIFPSYEDY
                     GIVPLEAQAWGVPVIALGKGGVLETVKENVSGVFFERQEEDSLVEGIKRFESSRFDAR
                     LIRDWAGEFNGERFIAQVRQFVQAP"
     gene            2639..4432
                     /locus_tag="P5516_07290"
     CDS             2639..4432
                     /locus_tag="P5516_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92275.1"
                     /translation="MNQQKNLTPNVPLPMPSAPLFQVLRRAYGYLRPYWKKTAGAYIA
                     LFAVLGFNTLIPQFIRWIIDNGIAGKQMSVLTWSVLALLAMTIIKGALNYFVGLLSET
                     ASQNVAFDLRNDIQRKLTQLSFSFHDQSETGELLSRAVQDVERVRFLTGRATLRIAEG
                     VLLLLITSIVLLVMDFKLGLLTLLTMPALVYQAVRFGSRFRPLSLQVQKQLAALTTAV
                     EQNLRGGRVVKAFAQEEAEIERFGITNNTWLQLSNQAARMQAFNVPMLFLIANLGMAI
                     IIFYGGRQYIEGIISMGTIVAFVSYVGNLIEPVRRLGMIIPAIAIAGAASERIFDVLD
                     FSSQVRDEPGAKAIKKINGRVRFEHVSFHYGRNQILKDISFDVQPGQVVALLGLTGSG
                     KSSIVNLIPRFYDPSAGRILVDEMDIRAMTLKSLRRQIGIVLQETTLFAATIRENIAF
                     GNEKATDEEVIAAASAAQAHEFIMQTSQGYQTRIGERGVTLSGGQKQRIAIARAILMN
                     PRILILDDATASVDTATEQLIHQALTRLIQGRTTFVIAHRLSTVRHADLILVLEGGTI
                     AARGTHEELLRSSSTYRAIYNQQLKKQEETG"
     gene            4429..6264
                     /locus_tag="P5516_07295"
     CDS             4429..6264
                     /locus_tag="P5516_07295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92276.1"
                     /translation="MSFSIGPTSSGSGPRTLLEDFGSVGEQSGEVYNPRVVRRMLAYL
                     APHRWRMVAALLLTLVEAGLTLLTPYLIKVAIDQYIIPANRQGLARIALTLTISFILL
                     FAASSFQRYIVSWVGQNVLTTLRADLFTKLQYLQQAYHDRHIVGVTVSRVINDVAEIN
                     ELLSQGIITLLGDLVVLIGIIIVMLSMSPRLALLTFTVLPIMLAATWLFSRQARKAFR
                     KTRSSVAAVVGDLAMDINGIRAIQAFTREKASQERFEQVNLKNRDAYINAMNLSFIFL
                     PGIEFLGMLATVIVLWFGGHYVMGGAVTLGVMVAFLSYVNRFFQPIQELSRIYTTFQS
                     AMAGGEQVVELLDTPLEIIEKPGAPELLPQNGEIRVTGVSFRYNPDSAEVLHDIHMVI
                     PAGKTAALVGPTGAGKTTIANLIARFYDTTAGSITIDGVDIRDVTINSLRRQVCVISQ
                     DPFLFSRSIAENIRFGNPAATDEAVMQAARQANAHDFIDRLPDGYNTRVLEGGVNLSQ
                     GQRQLLSIARALLTNPAVLILDEATANIDSVTEALLQDAIARMLKGRTAMVIAHRLST
                     VRNADLVFVIAEGKIVEQGTHNSLLKKNGLYRQLYERQFMDEPGT"
     gene            complement(6287..8014)
                     /locus_tag="P5516_07300"
     CDS             complement(6287..8014)
                     /locus_tag="P5516_07300"
                     /inference="COORDINATES: protein motif:HMM:NF012239.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dynamin family protein"
                     /protein_id="HRT92277.1"
                     /translation="MRILTPLQEDLLSRERSLLNDLRLKLLGYDASQEDVTSLGHSIS
                     QLDDLFLLVIVGEFNAGKSAFINALLGQKLLREGVTPTTSQINILRYGEKGAPSLVEE
                     GHTLLLYPVELLSELSIVDTPGTNAVIRQHEELTTHFIPRADLVLFVTSADRPFTESE
                     RVFMEQIRAWGKKVIIILNKTDLFQEQSELEQVVEFVQQNAQVLLNSTPQIFPVSSRL
                     ALRAKMGEPQFWQDSGFEALETYIHNSLDQAEQVRLKFLNPLGVGAHLTGKYYAKAEA
                     QRQILQEDVSMLQNVERQHEVYRNDMRKDFDFRMADVENIFYEMEQRGDAFFEDRFRL
                     VKVMDLLKKECMRSDFTREVVADLPEQVETRVNALIDWLVGCDLQQWKAVTSYLADRQ
                     REHKEKLIGEGINSHFTYDRERLLDAIGREAEQVVAGFDKHAEAAAIAENAKNAVAAS
                     AAVEVGAIGLGTLVTVLATTMSADVTGILAAGAVAALGLFIIPAKRRKAKSELHQKIS
                     NLRAELVDSLRQEFTKEIDLSLKRIEEAISPYTRFVRSESAHNEKILDELKYSQLAIN
                     QMRSQIESW"
     gene            complement(8416..8955)
                     /locus_tag="P5516_07305"
     CDS             complement(8416..8955)
                     /locus_tag="P5516_07305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92278.1"
                     /translation="MTTFNPEELKYYALIFGVILLVIVLYRKAIKKVNTRQEGKLKAQ
                     PGYKGAAARAPLEKPALTEEQFARIWKNLSYLILMAGLGNLYMAYTAVRSATQTTGLW
                     VWWIDAVFSTLAAAAAWMMWRKKEKLWVFIYFILLLVPIFLFMSIKGTPFKISALIHL
                     FPLVLLYFALKPIWAGMKQ"
     gene            complement(9109..10764)
                     /locus_tag="P5516_07310"
     CDS             complement(9109..10764)
                     /locus_tag="P5516_07310"
                     /inference="COORDINATES: protein motif:HMM:NF012686.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HRT92279.1"
                     /translation="MVLEGYQYAWLVFLFIALFFAVRIIVGVWAGRKVQDNTDYVVAG
                     RRLPIHMAAASIMATWFAAETLMGASETAYDYGFQGVVFDPFGASACLFLSGFLFVRL
                     MRRAKYLTVVDFFERRYNKLTVVLSVIAEMLTYFSWTAAQIVAGGAIAQGIFGLDPVW
                     GCVIVITIVAGYTMMGGMMADTLLDFFQMFLTAIGISLVFIFMLKAVGGFPGLIEGAG
                     STYVSQPFKLLPTKGEGYLGYTGPTGWMYWIAAWMSIGLGSVATQDLMQRSMAARNQA
                     TSVYGSYFAGVLYLFFGIMSPLVGIMAYQLNPGMEDTSYLLVHAAMGHMPAILTAIFI
                     AALVSALMSTSDSSLLAGASAVTENLIPLLTGKKLEGKKALWATRIMVLVNAALGLSI
                     ALWAHTIYELSVVAWTLLLVGLFTPFSFGMYWKKANSSGANAAFIGGFAVWITAAIFF
                     FKTGLGGQSTALVCGYAGEGAFSEPWWCAFWDAVYVASFVAFISAIILMVVVSLATQK
                     KDPAKQLVDYYGEPINMSPKLHLGILPIRNAFRKITEEEKENT"
     gene            complement(10814..12166)
                     /locus_tag="P5516_07315"
     CDS             complement(10814..12166)
                     /locus_tag="P5516_07315"
                     /inference="COORDINATES: protein motif:HMM:NF012428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRT92280.1"
                     /translation="MAQNFKEALDYSDRWLEIIEKAEVSEAEGKQIIEESKKNFAEFI
                     NKGWLDYRKSVTEAGDWACVEWTGRGAIFKDVLGREYIDCLGGYGMMDHGWSHPDVVK
                     AVAAQLRRTPMPSQELIDPLRGVLARLMANITPGDIKYSFFCASGTEAVEGAIKLAKM
                     YSKKSAFIVATKAFHGKTMGSLSMLGKADYRVPPGLLYAGQVYHVPYGDAAAVERQLQ
                     ICDQVGIGVAAVMMEPIQGEAGAIVPPDDFWPRIRKATREHGVLLIADEVQTGMGRTG
                     TMWGVEHWGVEPDIIAVAKSLGGGVMPVSSFCSTEEIWQVMMEPNPFIHTTTTGGGAL
                     ACSAAIAAINVALRDDFPKQAREKGDYFMKALKVLEKKYTNVYDYICGKGLLIGQHFK
                     DAEVGYKVAAGLFKRGVLVAGTLTSAHTIRIEPPIVIEYAQIDEVLNRLEDTLKEVSV
                     "
BASE COUNT         3024 a         3462 c         3045 g         2873 t
ORIGIN      
        1 ttgaaactca cgctgtgccg ccccatcacc tgccaccagc cggtaagccc tggcgtgaca
       61 cgatggataa tcgcggcaga atctcccatc ttctccagct ccgcaggcag ataggcgcgt
      121 ggtccaaccc agctcatttc tcccttgatc acattaagaa tttgcgggaa ttcatcaaag
      181 ctggtcttgc gcaggaatct gcccaccctg gtgacgcgcg ggtcatcctg aagtttatga
      241 tacctctcaa attcatcgcg caggttttca tccgcagcca gcaaatcctg cagtttttta
      301 tccgccccca caaccatcgt gcggaactta taaatattga atagttttcc ccctttgccc
      361 atgcgtgtct gtttatacag cactggcccc ggtgaatcta tccagatgat gagcgaaaag
      421 agcaggaaaa gcggaaaggt gaaaaccagg ctgagcaggc ttaaaaacag atccgccgcg
      481 cgtttgagaa acagtgcgaa aggattcaat aagttatact gtacctgcag tgcgggatgc
      541 ccgtccaaat ccatggtttt cattgccagc atgttcaaag gtgaatctcc taaaacatac
      601 agcagtttgg gaaagaccag gctcaaggtc tgtaaatatt tacgaaaacc aagccgggtt
      661 gaaaccgtgt aactggtgaa aatccccagg cgaatattcg ccttacggaa ttcatttagt
      721 tgtttttcag agaacggata gaccggcacc tcgtgaattt tttcctgcgg ggcgtctttg
      781 tcgagaacga tagccgcaat cgggcggtag gcaaggcgcc gcgctgattt caactgccgg
      841 atgaccatgg tcacatcttt gctgccgccg ataaccacag cgggttgacc ccaccagcgg
      901 cgcaatgacc cgcggttatg gagccataag cggaagctgg tgatcaacac cagcgcaaag
      961 caccagttga acaggaaaac caacctcgaa aaccgaggac cgtaaccgag gatgaagatg
     1021 atcatcgcca cgataatatg agagaaggag acgatataca caatttctct gaactcgacc
     1081 acccccgtgc ggccgtgacc agggtagaga ccaaagagaa gaaagatcac caggatgaag
     1141 aggattatta acaagaacaa tggggcaatc tggttaaaat cgaggacacc accgatgatg
     1201 gggataagcg ccttcctgag atagaacgaa aggataaaag ataaaatgat tgcggacagg
     1261 tcagaaagta acaatagccc ggaaaccaac cacggcttgt attcattcga gagagcgagt
     1321 tcagtaatgc cgttttgcgt attttgggtc aaagaaggtc ctcccctgct tatagagagt
     1381 attttactca aaaaacaagt gctccgttgc gttcatggtg cctgaacgaa ctgccgaacc
     1441 tgggcgataa aacgctcgcc gttaaattcc ccagcccaat cacggattaa gcgggcgtca
     1501 aagcggctgc tttcgaagcg cttgatccct tcaaccaggc tgtcttcttc ctggcgttca
     1561 aagaaaacac cactgacatt ttctttgact gtctccagca ctcccccttt acccaaagcg
     1621 atgaccggta ccccccaggc ttgagcttcc aatggcacga taccgtaatc ttcatagctg
     1681 gggaagatca acccgcgcgc gcggctgaat aacctctgca cctcttcatc gctcactctg
     1741 ccgacaaacc gaatcgttgg gccagccatt tgttccaggc gagctctttc cggtccatcc
     1801 cctaccacca gcagttgtct ccctaaacgg gtacaggctt ttaccgccag atccacccgc
     1861 ttgtgcgaga tcaggcgtga aaccgaaaga taatactccc ctcccttttc aatggccatt
     1921 tgcgggggaa tttccacagg cgggtagata acctgcgcct gcatattgta aaccttgtga
     1981 atctcattcg ccatgttgcg acaggtggtg gcaagcttgg ggatttcgcg cgtccgcttc
     2041 aaatcccagc gccgcagaag gggagtaacc catttcacca aagaggtggc tgggccgcgt
     2101 atgggcaggc tttcgggggt gtaagattca ggcttccaca acagccggaa aggagcgtaa
     2161 aggtaacatg cgtgtcttac ggtggaagtc ggacgaatga atttcgccag gtaagtagaa
     2221 gatgacagca cagcatcgaa gttgctgaaa tcctcgcgct ggatcaaggc gtaccacagc
     2281 gggtacagca gcttgaactg gcgctctgat ttaaccaggc gggagaaagg tagcgtattg
     2341 acctgcagcg ctttaaaacc ttcaaacgta ttcgcttgca ggtacacaga agtgtaaacc
     2401 ggcgcctgag gaaaagcctt tgccagccat aacaccatcc gctcggcgcc gcctgaaacg
     2461 cataatgcat catgtacaat cgcgatacgc atcaagctct cctgcccgca taaaatgggt
     2521 taagcaagta taacgtaaac cagtcaacga tacagctgtg gaaatactca cccaccgcgt
     2581 cggctttgaa tttttcaata gattgtagta ttctaaaaac tgtgcaaggt ggctcacttt
     2641 gaaccagcaa aagaacctga ccccaaacgt accgctgccc atgccttccg ccccgctctt
     2701 tcaagtgttg cggcgggctt acggctacct gcgcccctac tggaaaaaga ccgcgggagc
     2761 atacatcgcc cttttcgcgg tgttggggtt caatacactt attccgcagt tcatccgctg
     2821 gatcatcgat aatggcatcg ccggcaaaca aatgagcgtt ttaacctggt ctgtgctggc
     2881 gttgctggca atgaccatca tcaaaggggc gcttaattat tttgtagggc ttctatccga
     2941 aactgcttct caaaacgtgg cgtttgacct gcggaatgac atccagcgga aactgaccca
     3001 gttgtctttc tccttccacg accagtctga gaccggcgag ctgctctcac gcgcggttca
     3061 agatgtagaa agggtgcgct ttctcaccgg ccgggcaacg ctgagaattg ccgagggtgt
     3121 actgctgctg ctcattacca gcattgtgct gctggtaatg gattttaaac tcgggttgct
     3181 gaccttgctc accatgccgg cgctggtcta tcaggctgtg cgttttgggt ccaggttccg
     3241 accgctctca ttgcaggtgc aaaagcagct ggctgcattg accaccgcgg tagagcagaa
     3301 cctgcgcggc ggtcgggtgg tcaaagcatt cgcacaagaa gaagccgaaa ttgaacgatt
     3361 cggcattacc aataacacct ggctccagtt gtccaaccag gcggcgcgca tgcaggcgtt
     3421 taacgttcca atgcttttcc tgattgccaa cctgggaatg gccatcatca ttttctatgg
     3481 cggcaggcaa tacatagaag gcattatcag catgggaacc atcgtggcgt ttgtcagtta
     3541 cgttggcaac ctgatagaac cggtgcgaag gctgggcatg atcattcctg ccatcgcgat
     3601 cgccggcgca gcttctgaac gcatttttga tgtgctcgat ttcagctctc aggtgcgcga
     3661 tgaacctggc gccaaagcga taaaaaaaat caacgggagg gttcgttttg agcatgtttc
     3721 ttttcattac gggcgcaatc aaatcttaaa agatatctct ttcgatgttc aacctggtca
     3781 ggtggtggct ttattggggc tcaccggttc gggaaaatct tccattgtca atctaatccc
     3841 gcggttttat gacccaagtg cagggcgcat cctggttgac gagatggaca tccgcgccat
     3901 gaccctcaag tcattacgcc gccaaattgg catcgtgctg caggaaacca cattattcgc
     3961 cgccacaatt cgagagaaca tcgcctttgg caatgagaaa gccacagatg aagaggtcat
     4021 cgccgccgcc agcgcggcgc aggcgcatga gttcatcatg caaacttctc agggttatca
     4081 aacacggatt ggcgaaaggg gtgtcaccct ttctggcggg cagaaacagc gcatcgccat
     4141 cgcccgggcg atcttgatga acccgcgcat cctcattttg gatgacgcca ccgcttctgt
     4201 agacaccgcg accgagcagc tcattcacca ggcgctcacc aggctgatcc aggggcgcac
     4261 cacctttgtg atcgcccacc gcctttctac cgtgcgccac gctgacctca tcctggtgct
     4321 cgaaggcgga accatcgcag cgcgcggaac gcacgaggaa ctgctgcgca gctcaagcac
     4381 ttaccgcgcc atttacaacc agcagctgaa aaaacaggag gaaaccgggt gagtttttcc
     4441 atcggaccca ccagttctgg ttcaggaccc cgcaccctgc tggaagattt cggcagtgtt
     4501 ggtgagcaga gcggcgaggt ttacaacccg cgcgtggtac ggcgcatgct cgcttacctc
     4561 gcgccccacc gctggcgtat ggtcgctgcc cttctcttaa cactggtgga agctggtctg
     4621 accttgctca caccgtacct catcaaggtg gccatagacc agtacattat tcccgcaaac
     4681 cgccaggggt tagcgcgcat tgcactcacc ctcaccattt cgtttatcct tttattcgct
     4741 gccagctcgt ttcaacgtta tatcgtttca tgggttggac aaaatgtgct caccacgcta
     4801 cgcgctgacc tctttacaaa acttcaatat ctgcaacagg catatcacga ccgccatatc
     4861 gtaggcgtga ccgtgtctcg cgtgatcaac gatgtggcgg agatcaatga actgctttct
     4921 caagggatca tcaccctgct gggtgatctg gttgtgctca ttggcatcat tattgtcatg
     4981 ctgagcatga gcccccggtt ggcgctgctt acatttaccg tgctacccat catgctcgct
     5041 gccacgtggt tgttttctcg ccaggcgcgt aaagccttcc gcaagacccg ttcaagtgtc
     5101 gctgcggttg ttggtgacct ggccatggac atcaatggca ttcgcgccat ccaggctttc
     5161 acccgggaga aagcctctca ggaacggttc gagcaagtta acctgaaaaa tcgcgacgct
     5221 tacatcaacg ccatgaacct atcgtttatt ttcctgccgg gaattgagtt cctcggaatg
     5281 ctggcaacag tgattgtgct atggtttggc gggcattatg taatgggcgg cgcggtcact
     5341 ttgggggtga tggtagcgtt tttaagttac gtcaaccggt tcttccaacc cattcaggag
     5401 ctcagccgta tttacaccac ctttcagtcg gcgatggctg gcggcgaaca ggtagttgag
     5461 ctgctggata ccccgcttga aatcatcgaa aagccgggcg cgcccgaact gctcccgcaa
     5521 aatggagaaa ttcgggtgac cggtgtttca ttcaggtaca atcccgattc agcggaagtg
     5581 cttcacgata tccatatggt aatccctgcc ggtaaaaccg ctgcgctggt aggtccaacc
     5641 ggcgccggta aaaccaccat cgccaacctc atagccaggt tttatgacac cacggcgggt
     5701 tctataacca ttgacggcgt ggatatccgt gatgtcacca tcaactccct gcgcaggcaa
     5761 gtgtgtgtga tctcgcagga cccttttctc ttctctcgtt ccatcgcgga gaacatcagg
     5821 tttggcaacc cagccgccac ggatgaagca gtgatgcagg cggccaggca ggctaacgcg
     5881 catgacttta tcgaccgcct gccggatggc tacaacaccc gcgtactgga aggcggcgtg
     5941 aacctctcac agggtcaacg ccagttgctc agcatcgcgc gcgcgctgct gaccaatccc
     6001 gcggtgctca tcctggatga ggccacggct aatatcgatt cggtgacaga agcactgttg
     6061 caagacgcca tcgcgcgcat gctcaaaggt cgcacagcca tggtcattgc gcaccggctc
     6121 tcaaccgttc gtaacgcgga cctggttttt gtaatcgctg aaggcaaaat cgttgaacag
     6181 ggcacacaca acagcctgct gaaaaagaat ggcttatatc gccaattgta cgaacgtcag
     6241 ttcatggatg aacctggaac ctgaaattcg cttatccgga taaatctcac caactctcaa
     6301 tctggctgcg catctggttg atggctaatt gggagtattt caactcatcc aggatcttct
     6361 cattgtgcgc gctctccgaa cgcacaaaac gggtgtaagg agaaatcgct tcttcgatgc
     6421 gcttgaggct gaggtcaatc tcttttgtga actcctgcct taaagaatcc accagttcag
     6481 cgcgcaggtt cgatattttt tgatgcaact cgcttttggc tttacggcgt ttggcgggga
     6541 tgatgaacag acccagcgcc gccacggccc cagccgccag tatgccggtt acatctgcgg
     6601 acatggtggt agccagaaca gttaccagcg ttcccaaacc gatcgctccc acctcaactg
     6661 ccgcagaagc cgccaccgcg ttcttggcgt tttccgcgat ggccgccgct tcggcgtgtt
     6721 tgtcaaaacc agccaccacc tgctctgctt ctctgccaat ggcgtctaac agccgctccc
     6781 ggtcatacgt gaaatggctg ttgatgcctt caccgattag cttctccttg tgctcgcgct
     6841 gccggtccgc gaggtaagaa gtaaccgcct tccattgctg caaatcacat cccaccagcc
     6901 aatcgatcag cgcgttcacc ctggtttcta cctgttctgg caggtcagcc accacctcgc
     6961 gggtaaagtc tgaccgcatg cattctttct taagcagatc catcaccttt accagcctga
     7021 aacggtcttc aaagaaagcg tcaccgcgct gttccatctc ataaaagatg ttttccacat
     7081 ccgccatgcg gaaatcaaag tctttgcgca tatcattgcg gtagacttca tgctgccttt
     7141 ccacgttttg cagcatgctg acatcttcct ggaggatttg ccgttgagcc tctgccttcg
     7201 cgtagtattt acccgtcagg tgcgccccca ccccaagcgg gttaaggaac ttgagtcgta
     7261 cctgttcggc ctgatccaaa ctgttgtgaa tgtaggtttc caacgcttca aaaccactgt
     7321 cctgccagaa ctggggttct cccatctttg cgcgcagcgc cagcctggag cttaccggaa
     7381 agatttgcgg cgtgctgttc agcagaacct gggcgttttg ttgaacaaat tcaaccacct
     7441 gttcaagttc gctttgctcc tggaacaggt cggtcttatt gaggataatg atcaccttct
     7501 tcccccaggc gcggatttgc tccataaaaa cccgttcact ttcagtaaag gggcggtcag
     7561 cggaggtgac gaacaggacc aggtcagcgc gggggataaa atgcgtggtc agttcctcat
     7621 gctgccggat gaccgcgttg gtaccgggag tatccacaat gctcagctcc gacaacagct
     7681 caaccggata cagcagcagg gtgtgccctt cttccaccag gctgggcgcg cctttctctc
     7741 cgtagcgtag aatgttgatc tgagacgttg ttggcgtcac tccttccctg aggagctttt
     7801 gacctaacag cgcgttaatg aacgcgctct tgccagcgtt gaattcgccc acaatcacca
     7861 gcagaaaaag atcatcgagt tgagagatgg aatgcccgag agaggtcaca tcttcttgag
     7921 aggcgtcata acccaataac tttaaacgca ggtcattgag caggcttctc tcacgagaca
     7981 gtagatcttc ctgcaatggt gtcaagattc tcatagcgct ccttccttct ctttaccagc
     8041 tttcatgttg ctgcccgctc tggaatacat atccattcca gcctttgtgt gcaggcaagg
     8101 ttattatagc cttattgcca acgcctttaa cagggtggaa atgagcaacc ctgaaccaaa
     8161 ccgccgcaga ttggaacaga gtcgcgaagg tggtttctca ttgagttacc ccatacgcct
     8221 gaacacaggg aggttgttaa tccttctgct gtccttccat cacccggggc ttaccacggc
     8281 gggtgccccc gaagctggta gcgaaattgt cattcgaaaa caaacaaaat aatgccccgc
     8341 gcgtgaccgg taaaaagaac actctggtcc ttcaggcaat tccttacgcg aagttattat
     8401 cggttcgcct gattattact gtttcatgcc agcccagatc ggcttgagcg caaaataaag
     8461 cagaaccaaa gggaaaaggt ggatgagcgc gctgatcttg aagggagtgc ccttgatcga
     8521 cataaacagg aagatgggga caagcagcaa aataaaataa ataaataccc aaagcttttc
     8581 tttcttgcgc cacatcatcc atgcggctgc ggcagccagg gtgctgaaaa ccgcgtcaat
     8641 ccaccacacc cataatccag ttgtttgagt ggcgctcctg accgctgtgt aagccatata
     8701 gaggttcccc aggccagcca tcaaaatcag gtaagagaga tttttccaga ttcgagcgaa
     8761 ctgctcttcg gtcagggctg gtttttccag cggcgccctg gccgcagccc ctttgtaacc
     8821 gggttgagcc ttcagtttgc cttcttgcct tgtattgacc tttttgatcg cttttcgata
     8881 aagcacaatc accaacagga tgactccaaa aataagcgcg taatatttga gctcttcagg
     8941 attaaacgtg gtcatggttg cctactgacc ctccgattac aggaagtatc cacacaggga
     9001 tttggcggcg cccaaagcca gtgaccttga gcgcctttca ttatacacac ccaaattcag
     9061 ttgagcagcc tgctgatgat ctctttccgc ttgtaacgca ttggaaaatc aggtattttc
     9121 cttttcctcc tcggtaatct tgcggaaggc attcctgatg ggcaaaatac ccaaatgcag
     9181 ttttgggctc atattgatcg gttcaccgta gtagtctacc agttgcttgg cgggatcctt
     9241 cttttgcgtt gcgagagaaa ccacaaccat caggatgatc gcggatatga atgccacgaa
     9301 agacgccaca tacaccgcgt cccagaaggc gcaccaccag ggttcagaaa acgcaccttc
     9361 accggcgtat ccgcagacga gcgcagtgga ttgccctcct aaaccagttt taaagaagaa
     9421 gatagccgct gtaatccaga ccgcgaaacc tccaataaaa gccgcgttcg cccccgatga
     9481 attggctttc ttccagtaca taccaaagga aaaaggagtg aaaagaccca ccaacagcag
     9541 cgtccaggcc acaacggaga gctcatagat cgtatgcgcc cacaaagcaa ttgaaagccc
     9601 gagtgcagca ttgacgagca ccatgatcct ggttgcccac agcgccttct tcccctccag
     9661 cttcttaccg gtgagcaatg gaatcaggtt ttccgtcaca gctgaagccc ccgccagcag
     9721 cgagctatca gaagtgctca tcaacgccga aaccagagca gcaataaaaa tagcagtcag
     9781 gattgctggc atatgcccca tggctgcatg caccagcagg tagctggtgt cttccatgcc
     9841 tggattcagt tgataggcca taataccgac cagagggctc atgatgccaa agaacagata
     9901 gagcacccca gcgaaatacg aaccgtaaac cgaagtcgcc tggttgcggg cggccatcga
     9961 acgctgcatg agatcttgtg tggccacgga gccaagtcct atagacatcc aggcggctat
    10021 ccaatacatc catccggtgg ggccggtata acccaggtaa ccttcaccct tggtcggcag
    10081 cagtttaaag ggttgagaca catacgtcga ccccgctcct tcgatcaatc cggggaagcc
    10141 gccgaccgct ttaagcataa aaatgaacac cagcgagatg ccaatagccg ttaagaacat
    10201 ctggaagaaa tccagcagcg tatccgccat catgccgccc atcatggtat agccggccac
    10261 aatcgtgata acgattacgc acccccatac cgggtcaagc ccaaaaatac cttgggcgat
    10321 ggcgccaccc gccacaattt gcgcggcagt ccaggagaag taggtcaaca tttccgcaat
    10381 gaccgagaga acaaccgtca gcttgttgta gcgtctttca aagaagtcca ccacggtcag
    10441 gtacttcgcg cgccgcatca ggcggacaaa aaggaaacct gaaaggaaaa gacaggctga
    10501 agcgccaaag gggtcaaaaa ccaccccctg gaagccgtaa tcataagccg tctctgaagc
    10561 gcccatcaag gtttcagctg cgaaccaggt agccataatg gaagccgcag ccatgtgaat
    10621 gggcagtctt cttcctgcca ccacataatc tgtattgtct tgaacttttc gaccggccca
    10681 cacgcccacg atgatgcgca cggcgaagaa gagcgcaatg aacagaaaaa ccagccaagc
    10741 gtattgataa ccttcaagaa ccattcgacc cttcctttct gacctgagac ggataagttg
    10801 aggtatcaat cattcagacg cttacctctt tcaatgtatc ttccaggcgg ttcaaaacct
    10861 catcaatttg agcgtattca ataacaatag gcggttcaat gcgaatggta tgggcgctgg
    10921 ttagcgttcc agcaaccaga acgccgcgtt taaagagccc tgccgcgacc ttgtacccga
    10981 cttccgcatc cttgaagtgt tgcccgataa gcaatccttt tccacagatg tagtcgtata
    11041 cgttcgtgta ttttttctcc agcaccttga gagccttcat aaagtaatcg cctttttcgc
    11101 gtgcctgctt cggaaaatca tctcgcaacg cgacattgat ggcggcgata gccgctgaac
    11161 aggccagcgc gccgccgcca gtggtggtgg tatgaatgaa cggattcggc tccatcatca
    11221 cctgccagat ctcttctgta gagcagaagg agcttaccgg catgacccca ccgcccaaag
    11281 atttggcaac cgcaatgata tcaggttcca ccccccagtg ctccacaccc cacatggttc
    11341 cagtgcgccc catgcccgtt tgcacttcat cggcaataag cagcacacca tgctccctgg
    11401 tcgcttttcg aattcgcggc cagaagtcat ccgggggtac gatcgcgccg gcttcaccct
    11461 ggatgggttc catcatcaca gccgccacgc cgatacccac ctggtcgcaa atttgaagct
    11521 ggcgctcaac cgcagccgcg tcgccatatg gaacatggta aacctgaccg gcgtatagca
    11581 agccaggcgg tacgcggtaa tctgctttgc ccagcatgga gagcgaaccc atggtcttgc
    11641 cgtggaaagc cttggtcgca acaataaagg ccgatttctt tgagtacatt ttggccagtt
    11701 tgatcgcgcc ttctactgct tcagtgccgc tggcgcaaaa gaaagaatac ttgatatcgc
    11761 ccggggtgat attggccatc agcctggcca acacgccgcg cagtgggtcg atcaattcct
    11821 ggctgggcat gggtgtgcgg cgcaactgcg ctgccaccgc ctttaccaca tcagggtggc
    11881 tccacccatg gtccatcatg ccgtaaccgc ccaggcagtc aatgtattca cgacctaaca
    11941 catctttaaa aatcgccccc ctgcctgtcc attccacaca ggcccaatcg cctgcttcgg
    12001 taacggattt tcgataatcc aaccagccct tgttaataaa ttccgcgaaa tttttcttgc
    12061 tttcttcgat gatctgtttg ccttcggctt cgctcacctc ggctttttcg atgatttcca
    12121 accagcgatc tgaatagtca agcgcttcct taaaattctg tgccatctct cctccttcga
    12181 tcttcaacta ttttgggtgt caaatagaac aagagagcag ggaaattgct gatttcgtgc
    12241 aagtgcgggt tggagaatac cgacaaacca ggagtcaatg ggaaaatgga tgtttttcct
    12301 attgatttat tatgacaaga aaaaacatcg tgtcaagtat tttttattaa caattttccg
    12361 tagagcatct cctattggaa cacgttcctt ttttctaatc ttaa
//
LOCUS       DAOTLW010000047        11592 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_5952_length_11592_cov_10.756609, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000047 DAOTLW010000000
VERSION     DAOTLW010000047.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11592)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11592
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_5952_length_11592_cov_10.756609"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /gene="selA"
                     /locus_tag="P5516_07320"
     CDS             complement(<1..662)
                     /gene="selA"
                     /locus_tag="P5516_07320"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRT92281.1"
                     /translation="MIDLRKLPSVDRLLLHAHAAPMLDCYGRSLVTTNIQNVLESIRQ
                     NPQAETFEPTEEAILERVKNQLIQALTPTLTRVINASGVVLHTNLGRAPLSRSAQQAM
                     SEIAGGYASLEYDLEKGKRGYRSVHASGLLRRLTGAEDALVVNNNAAAVLLCLSALAS
                     RRAVVISRSQLVEIGGGFRIPEVMKQSGARLVEIGTTNRVNLGDYESALRDGAALLMR
                     AHT"
     gene            complement(659..1069)
                     /locus_tag="P5516_07325"
     CDS             complement(659..1069)
                     /locus_tag="P5516_07325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92282.1"
                     /translation="MKDTQPPRMLFMPVLTTSIILVLTSTVGLMGLFLFTVPTLGPRW
                     LLFFFVTLLASGLALPAIFYLHLRFPSNPPVTSKVIIREAAWWGIYADILLWMQFGRV
                     LNFALAIFIAIGFIAIELLIRMRELNRFPSRMKP"
     regulatory      1238..1338
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            1412..>3827
                     /locus_tag="P5516_07330"
     CDS             1412..>3827
                     /locus_tag="P5516_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HRT92283.1"
                     /translation="MDRTFTNRQYLDALQSKVMVFDGAMGTNLQAQNLTADHFGGEKT
                     NGCNDYLVITYPQAVEKVHRSFLSVGVDVIETCTFRSNRLTLSEYGLSQEVTAINQAA
                     AALARRLADEYSQTGQPRYVAGSIGPSGKLPSMSDAELSNITFDELTSIFKQQAEGLI
                     LGGVDLILIETSQDILEVKAAIHGIQQAFSSSGVFLPIQAQVTLDTTGRMLLGTDITA
                     VLAILEGLPIDVIGLNCSTGPDFMREPIRFLGEHSTLPVSCIPNAGLPLNVNGQAVYP
                     LEPEPFAQALYNFVVNHGVSVVGGCCGTTAAHLARLVEKVRAIQPGPRLRISSARLSS
                     AMQAQPMQQEPPPFFIAERLNTQGSHVFKNMVLENDFESILTLAQQQMESGAHGLDLC
                     VALTERADEKETMQRLIKTIAPVINLPLVIDTTEVDVLETALKTAPGRCLINSTHLEG
                     GRQKADRIFGLAKTHNAAVIVLTIDEQGMAKTRERKLEVARRIYDIAVKEHGLAPHDL
                     VFDDLTFTLATGADEFTNSAIETLEGIRLIKAALPGVLTSLGVSNVSFGLAANARKVI
                     NSVMLYHCVQAGLDMAIVNPATITPYPDLSPEERELAEDLIFNRKPEALQRLIEFFQA
                     AGPSSAQQNSQQEALSMMTPGERLHWKIVHRAKEDLETDIDTLLQMREKEVSRNDAAV
                     GILNHVLLPAMKDVGDKFGRGELILPFVLQSAEVMKKAVSYLENFLERKEGASKGKLL
                     VATVYGDVHDIGKNLVKTILSNNGYNVVDLGKQVPADTIINRAVEEKADAIGLSALLV
                     STSKQMP"
     assembly_gap    3828..3927
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3928..4728
                     /locus_tag="P5516_07335"
     CDS             <3928..4728
                     /locus_tag="P5516_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin B12 dependent-methionine synthase
                     activation domain-containing protein"
                     /protein_id="HRT92284.1"
                     /translation="AALIPSPPGYGVRIVEDMPLEIVFQHLAVNELFRLSWGAKNAHG
                     AEWEKIKTEYEKRLALMQKQAIAEKWLKPRGVYGYWPAQSDGNDLLIYDGDSVEKGNP
                     IVINRFTFPRQSSGEFLCLADYFASVDSGRMDVVAFQVVTVGKEASERFDRLQAVADY
                     AEAYFSHGLSVQTAEATAEYLHRHIRHELGLSENQGKRYSWGYPAIPELEDHKKVFAL
                     LPAEAALGMSLTSACQLVPEQSTAAIILHHPQAKYYSLGETRVEQLMK"
     gene            4739..6613
                     /locus_tag="P5516_07340"
     CDS             4739..6613
                     /locus_tag="P5516_07340"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HRT92285.1"
                     /translation="MINQATLLNLLAKKRALFSDGAMGTMLSQNLPTGLTCLDYANVE
                     YPRVVESIHREYILAGSDMIQTNTFGANRYKLAQHHLENQLEQINTAGAQIARQAVSA
                     SGREVLVAGDIGPLGVRIAPFGRVQLPEAHEAFSQQATALIKAGVDLIIIETMTDLYE
                     TVEALRAVREISPQLPVIASMTFTRDNRTLLGDPPTRVSQQLHAAGADVIGVNCSGGP
                     NQILNILRQMRRAVPDAIFSVMPNAGWPEQVEGRIMYPAAPEYFGTYALSFWQSGASI
                     IGGCCGTTPAHIAAMVQAFQQSPGLERSAIEEIALSATRQPEDEGALPPTKLAQKLRD
                     GRFVIAVEMDPPRGLSTHKLIAGASLLSEVGADVIDVADSPMARMRMSPWAVCSLIQR
                     NVGIETVLHFPTRGRNVLRVQGDLLAAHALDVRNIFVVMGDPTSIGEYPDAMDHYDLV
                     PSGLIKLVKQGFNAGVDHAGSGIGQPTSFFVGCALNLKAADMEAELKNLRRKLKAGAD
                     FILTQPIYHPALVTDFLQQYESQFDKLPVPLIVGLLPLASARHAAFLQAEVPGIEIPQ
                     SIRDLMAVSGDQGAQTGIRLTIELIQNIKPMVQGIYLMPAFQRYDYAAEIIEKTRTAE
                     "
     gene            6661..7392
                     /locus_tag="P5516_07345"
     CDS             6661..7392
                     /locus_tag="P5516_07345"
                     /inference="COORDINATES: protein motif:HMM:NF024258.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HRT92286.1"
                     /translation="MKILVISDVHANLTALEAVLADAGNNYEVVWCLGDLVGYGPDGN
                     QCVQRIRELPNLLCIRGNHDLAICGDADLSIFNHEAGEAIIISRRMMSPETLQYLRSL
                     PEKQVSGQATLIHGSPRNPVWEYILDVSIAAENFASFDTQLAFVGHSHLPLMFTADPQ
                     SGLVERAYRYPGVTTPVTTRAILNPGSVGQPRDNDPRASYGIFDPQNMTWEIHRVPYD
                     IPSVQQRIIAAGLPEKHARRLSAGW"
     gene            7466..8458
                     /locus_tag="P5516_07350"
     CDS             7466..8458
                     /locus_tag="P5516_07350"
                     /inference="COORDINATES: protein motif:HMM:NF025616.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HRT92287.1"
                     /translation="MKTKSVLIGIVILALTLSGCASKSYTANNLRSGGVEDYAMKAAP
                     MVEPMEPMLDSYAESAGSPGIAAAGVESVQMIMMNASLSIAVDDPAATLRDVQQLAKG
                     MGGYTVSSNLYKSYTSSGVEVPQANLIIRVPAECLDEAMQKIKDMTGDPKKYTLNESV
                     SGQDVTKEYTDLKSRLRNLEEADAKLSEFYENAANTEDALSIYSQKMQVTEQIEIIKG
                     QIKYYDESVSTSSITLQIQSKETIAPITVAGWQPSGVARDALQALLDFGKGLVNFLIW
                     LVILVVPIVLLFGLPIFFLVRWLKRRSTARKLAALERRAQENKSEPTPPIPTKK"
     gene            complement(8505..10847)
                     /locus_tag="P5516_07355"
     CDS             complement(8505..10847)
                     /locus_tag="P5516_07355"
                     /inference="COORDINATES: protein motif:HMM:NF014156.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HRT92288.1"
                     /translation="MEQLNSVSLMLQVNPDSGTFSIQARDPRFPRLENIQLGCEYTFA
                     GKQQRCGQDGWQVNGLAHHLERAQAHGEMDCLTVALEPDANKVSLRLSFALMREYPLV
                     LWKIQVTNHGKEDVEINRITLLRVVDNTHYLKVRRPDDLGFFSNGWQSWSPSRWYSGN
                     QRMTISRLYALQRPMIYNPGTPTPVKKGVFSSDMFAVVGDRATRAGYLLGFLSQLNHF
                     GSILADFNTGEISMWANGDGACLEAGKSMQTDWAVFTPVLLDHRAPLETYIEAVGREN
                     HARVPAQTPVGWCSWYHFYTSVTARNIIENLDTILERQEELPIELVQIDDGFEKQVGD
                     WLEFRSTFPEGVAPLAASISGGGLLPGLWLAPFIVHPGADLLHKHPDWMLRKANGRLV
                     NAGLGWNALTTALDLTKPPALQYTAEVVHTAVQKWGFPYLKLDFLYAAALKGHYYDRS
                     KTRAQVMRMGMEALRQAAGEQTTLLGCGAPLGSVIGVVDTMRIGPDVSEEWLPVFKGV
                     QIGLRREPSMPSTRNSIHNILTRANLNRRWWVNDPDCLLVRADTSLTQEEVRSLATVI
                     ALTGGSLLLSDDLPGVPPERIRLAEALIPVMGEEVRVLDWFDNATPSRLRVDMDNETG
                     GWHLLGGFNWSEQTADVMISASEYGLEEGEYLFREFWSGKTGMISGTGGHVFPGIPPH
                     APVLLAVRRFVGEKALYLGSDLHFTQGAEVAEWLEKDGSLEMTLRLPRTAIGQVFFHL
                     PGKPQAAFVDGKPVYLHPMEQGIYRLPVVVEGFCNVRIMR"
     gene            10954..>11592
                     /locus_tag="P5516_07360"
     CDS             10954..>11592
                     /locus_tag="P5516_07360"
                     /inference="COORDINATES: protein motif:HMM:TIGR00573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HRT92289.1"
                     /translation="MVAIDIETTGMDAQRDAIIEIAAVRFNGHRVEGEWTKLINPGRP
                     IPRQITQLTGITDQMVLHAPPLQAVIQELVDFVGNDIVVGHNVRFDLSFLQRQKVLLY
                     NKVIDTYEMAAIALPGNSRYNLGTLGVTCNILIPNSHRALDDARLAQAVYFSLSQKLL
                     ELPIEILDEIVRMSEGIEWDGEVAFHLALQAKGRLPIEARKASQDDFGLLFAG"
BASE COUNT         2711 a         3295 c         3032 g         2454 t
ORIGIN      
        1 gtgtgcgcgc gcatcaacaa cgctgcgccg tcacgcaacg cgctttcata gtcccccagg
       61 ttgacccggt tggtggtgcc aatttcaacc agtcgggcgc ctgactgctt catcacttcc
      121 gggatgcgga atcctccgcc aatttcaacc agctgcgaac gcgagataac caccgcccgc
      181 ctgctggcca gggcagataa acacaacaaa accgcggcgg cgttgttgtt taccaccagc
      241 gcgtcctccg ctccggtaag ccggcgcaac agcccgctgg cgtgcacgct ccggtagccg
      301 cgttttcctt tctccaggtc atactccagg ctcgcatatc ccccggcgat ctcgctcatc
      361 gcttgctgcg cgctgcggct aagcggagcg cgacccaggt tggtgtgtaa aaccaccccg
      421 ctggcattga tcacccgggt aagcgtggga gtgagcgctt gtatcaactg gtttttaacc
      481 cgttccaaaa tggcttcctc ggttggttca aaggtttctg cctgcggatt ctggcgtatg
      541 ctttcgagca cattctggat gttcgtggta accagtgatc gtccatagca atccagcatc
      601 ggggcagcgt gcgcgtgcag caagagcctg tcaacagagg gcagttttcg cagatcgatc
      661 atggtttcat tcgagaaggg aagcggttca gttcgcgcat acgaatgagt aactcaatcg
      721 caatgaaccc gattgcgatg aagatcgcca gcgcaaaatt caacactctg ccaaattgca
      781 tccatagcag aatatctgcg taaatgcccc accaggcggc ttcgcggatg atcaccttgg
      841 aggttacagg cgggttggaa ggaaatcgca gatgcaggta aaaaatcgcc ggaagcgcca
      901 acccgcttgc cagcagagtg acgaaaaaga acaacaacca cctggggcca agcgtcggca
      961 cggtgaaaag gaacaaaccc atcaacccga ctgtcgaggt cagcaccagg atgatgctgg
     1021 tggtgagcac cggcataaaa agcattcttg gaggttgagt gtctttcatg cgcagatgag
     1081 gttccttttc aattattgat aatccattat atcaattgcg ccggttaatg aacaacttct
     1141 gcgcgaaaaa acctaatgcc tgttgttgtt caggctcttg acctggaagc gcatttgatt
     1201 catggtaaaa tctagccatt acaggggatt agtgacccgc tcatcaagag aggtggaggg
     1261 accggcccgc tgaaacctcg gcaaccgctc acgcagactg gtgccaactc cggcagaagg
     1321 gattctgaaa gatgagagag aaagcaccac cttcaaacaa cctctctcgc gagaggttgt
     1381 ttttatcagt aaatcgctca aggagaccat aatggatcgt acattcacca accgtcaata
     1441 cctggacgcg ctgcaaagca aggtaatggt gtttgatgga gcaatgggca ccaacctgca
     1501 agcccagaat ttaactgccg accatttcgg cggcgaaaag accaacggct gcaacgatta
     1561 cctggtcatc acttaccccc aggcggttga aaaagttcat cgctctttcc tctcagtcgg
     1621 cgtggatgtg attgagacct gtaccttccg ttcaaaccgt ttaaccttaa gtgaatacgg
     1681 gttatcacaa gaggtgaccg ccatcaacca ggctgcggct gctttggcac ggcggctggc
     1741 agacgaatat tctcaaaccg ggcaaccacg ctatgtagcc ggatccatcg gtccctctgg
     1801 caaactgccc tctatgagcg acgctgaact atccaatatc actttcgatg agctcacctc
     1861 aatcttcaag cagcaggcag aaggattgat cctcggtggg gttgatttga tcttaattga
     1921 aacctcgcag gatatcctcg aggtcaaagc cgccatccat ggtatccagc aggctttttc
     1981 cagcagcggg gtatttctgc cgattcaagc ccaggtgacc ctggatacaa ccgggcgtat
     2041 gctgctggga acggacatca cggctgtgtt ggcgatactg gaaggcctgc ccatcgatgt
     2101 gatcggatta aactgctcca ccggaccgga ttttatgcgt gaacccattc gcttcttggg
     2161 cgagcactcc accctgccgg tttcctgcat tccaaacgct ggtttgcctt tgaacgtgaa
     2221 cggtcaggcg gtttatccgc tggaaccgga gccgttcgct caggcgcttt ataatttcgt
     2281 ggtcaaccat ggcgtcagtg tggtcggagg gtgctgcggt acaacagcag cgcatctcgc
     2341 ccgcctggta gaaaaagttc gcgcaatcca gcccggccct cgacttcgta tttccagcgc
     2401 gcggctctcc tcagccatgc aggcgcagcc gatgcaacag gaaccaccgc cttttttcat
     2461 cgcggaacga ttgaacacac agggcagcca cgtatttaaa aatatggtgc tggagaatga
     2521 tttcgaaagt atccttaccc tggctcaaca acaaatggag agcggcgccc acgggcttga
     2581 cctgtgcgtg gcattgactg aacgagccga cgaaaaagaa accatgcagc ggctgatcaa
     2641 gaccatcgct ccggtcatca atcttccgct ggtcatcgat accaccgaag tggacgtgct
     2701 ggaaacagcg cttaaaacag cgccaggacg ctgccttatc aactcaaccc acctggaagg
     2761 cgggcgtcaa aaagctgacc gcatcttcgg gttggcgaaa acacataacg cggcggtgat
     2821 cgtgctcacg attgatgagc agggcatggc gaaaaccaga gagcgcaaac tggaggtcgc
     2881 caggcgcatc tacgatatcg ctgttaagga acacgggctg gcccctcacg acctggtttt
     2941 tgatgatctc accttcaccc tcgcgactgg agcagatgag ttcaccaact cagcgattga
     3001 aacccttgag ggcatccgcc tgatcaaggc tgcgctgccc ggtgtgctga cctctcttgg
     3061 cgtcagcaat gtgtctttcg ggctggcagc gaatgcgcgt aaggtcatca acagcgtgat
     3121 gctttaccac tgcgttcagg cgggattgga tatggccatt gtaaacccgg cgaccatcac
     3181 gccttacccc gacctcagcc cagaagaacg ggagctcgct gaagatttga tcttcaaccg
     3241 caaacctgaa gcgcttcagc gcctgattga attcttccag gccgcgggcc ccagcagcgc
     3301 ccagcaaaat tcacagcaag aagcgctttc catgatgaca cctggcgaac gtcttcactg
     3361 gaagatcgtg caccgcgcga aagaagacct cgagaccgac attgacaccc tgctgcagat
     3421 gagggaaaag gaggtcagcc gcaacgacgc cgcggtaggc atcttgaacc atgttctgct
     3481 tccagcgatg aaggatgtgg gtgataagtt tggcaggggc gagctgatcc tgccgtttgt
     3541 gctgcaatcc gccgaggtga tgaaaaaggc ggtttcctat ttagagaact tccttgaacg
     3601 aaaggaaggt gccagtaaag gtaagctcct cgtggcgacg gtttacggag atgtgcatga
     3661 tattggcaaa aacctggtca agaccattct ttccaacaat ggctataacg tggtcgacct
     3721 cggaaaacag gtaccggcgg ataccatcat caaccgtgca gttgaagaaa aagcggacgc
     3781 cattggtttg agcgcgctgc tggtcagcac cagtaaacag atgccccnnn nnnnnnnnnn
     3841 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3901 nnnnnnnnnn nnnnnnnnnn nnnnnnngcc gcgctcatcc cctctccacc gggctatggt
     3961 gtgaggattg tggaagatat gcccctggaa atcgtttttc aacacctggc ggtcaacgaa
     4021 ctgttccgtt tatcctgggg agcgaagaac gcgcatggcg cggaatggga aaagataaaa
     4081 acggaatacg aaaagcgtct ggccctgatg cagaaacagg cgatcgctga gaaatggctg
     4141 aaacccaggg gagtgtacgg ttactggcca gcgcaatcgg atgggaatga cctcctcatt
     4201 tatgatggag attctgtgga aaagggaaac cccattgtga tcaaccgctt cactttcccc
     4261 cggcagtcca gcggagagtt cctctgcctt gcagattatt tcgccagcgt ggattctggc
     4321 aggatggacg tggttgcttt ccaggttgtg accgtcggca aagaagccag tgaacgtttc
     4381 gaccgtctgc aggctgtagc agactatgcc gaagcctact tttcgcatgg gttatcggtg
     4441 cagaccgcag aagcgaccgc cgagtacctc caccgccata tccgccatga acttgggctc
     4501 agcgaaaacc agggcaagcg ttattcctgg gggtatccgg ccattcccga gctggaagat
     4561 cacaagaagg tgtttgcctt attgcccgcg gaagcagccc tgggcatgag cctgacctcc
     4621 gcctgccagc tggttccaga acaatccacc gcggctatta tcctccatca cccccaggcg
     4681 aagtattaca gccttggtga aacccgcgta gaacaattga tgaagtaaac ggtgtcgaat
     4741 gatcaatcaa gcaacccttc tgaacctcct ggcaaaaaaa cgcgccctgt tttctgacgg
     4801 cgccatgggc accatgctga gccagaacct gcccaccggc ttaacctgtc tggattacgc
     4861 caacgtggaa taccccaggg tggtcgaatc gatccaccgt gaatacatcc tggccggttc
     4921 tgacatgata cagaccaaca ctttcggcgc gaacaggtac aaattggccc aacaccactt
     4981 ggagaaccag ctggaacaga tcaacaccgc gggcgcgcag attgcccgcc aagcggtgtc
     5041 tgccagcggc agggaggtgc tggtcgctgg tgatatcggc ccactggggg ttcgcattgc
     5101 gccttttggt agggttcaac tgccagaagc ccacgaagcc ttttcacagc aagccacggc
     5161 gttaatcaaa gccggcgtcg acctgatcat cattgaaacg atgaccgacc tttacgaaac
     5221 tgttgaagca ctgcgggcgg tgcgtgaaat ttcaccgcag ttaccagtca tcgcctcgat
     5281 gaccttcacc cgcgataacc gcactctgct gggcgatccc cccaccaggg tgtcgcaaca
     5341 gttacacgct gccggcgcgg atgtgatcgg cgtcaactgc tctggcggtc ccaaccagat
     5401 tctcaacatt ctcaggcaga tgcgccgcgc tgtgccggat gcgatttttt cagtgatgcc
     5461 caacgccggt tggcctgaac aggtggaggg tcgcatcatg taccccgccg ctcccgagta
     5521 tttcggcact tacgcgctct ccttctggca gtccggcgcc agcatcattg gcggctgctg
     5581 cggcaccact cctgcccaca tcgccgccat ggtacaggct tttcaacaat cgccaggatt
     5641 ggaacgctca gcaatcgaag agatcgcgct atccgcaacc aggcagccgg aagatgaagg
     5701 cgccctgcct cccacaaaac tggcgcaaaa actccgcgat gggcgattcg tcatcgctgt
     5761 tgaaatggat ccaccccgcg ggctttccac ccacaagtta attgccggcg cgagcctgct
     5821 gtctgaagtc ggcgcggatg tcatcgatgt ggcagacagc cccatggcgc gcatgcgcat
     5881 gagcccctgg gcggtttgca gcctgatcca gcgcaatgtg gggatcgaga ccgtgttgca
     5941 ctttccaacg cgcgggcgca atgtgctgcg cgtgcagggt gacctgctgg cagcccacgc
     6001 gctggatgtg cgcaatattt tcgtggtgat gggtgatccg acttctattg gtgagtaccc
     6061 tgacgcgatg gatcattatg atctggttcc ttccgggttg atcaaactgg tcaaacaggg
     6121 gttcaacgcc ggtgtggacc atgccggaag cggtattggg cagccgacca gcttttttgt
     6181 cggctgtgcg cttaacctga aagccgcgga tatggaagca gaactgaaga atctgcgccg
     6241 aaaactgaaa gccggagcgg attttatcct cacccagccg atctatcacc ccgctttggt
     6301 aactgatttt ttgcagcaat acgagtctca gtttgataaa ctacccgttc cattaattgt
     6361 ggggctgctg ccgctggcca gcgcgcgcca cgccgctttc ctgcaggcgg aagttcccgg
     6421 aatcgaaatt ccgcaaagca tccgtgacct gatggcagtc agcggtgacc agggagcgca
     6481 gaccggtatc cgccttacca tcgaactcat ccagaatatc aaaccaatgg tgcagggcat
     6541 ctacctgatg cccgcattcc aacgctacga ctacgccgcc gagatcatcg aaaaaacaag
     6601 aacggctgaa taatgtaaaa taaacccatt gaaccatcaa aaaaccgacc aagggaaaaa
     6661 atgaaaatcc tggtgatttc tgatgtgcac gccaacctga ctgcgcttga agccgtcctc
     6721 gcggatgccg gcaataatta tgaggttgtg tggtgcctgg gagacctggt tggttacgga
     6781 ccagacggca accaatgcgt acagcggata cgcgagttgc ccaacctgct ttgcatcagg
     6841 ggcaatcacg acctcgctat ctgcggtgac gctgacctga gcatcttcaa tcacgaagcc
     6901 ggagaagcga ttatcatttc ccggcgcatg atgtcgccgg aaacgctgca atacctgcgc
     6961 agtctgcctg aaaaacaggt gtcaggtcag gcaacgctta tccatggcag cccgcgaaac
     7021 ccggtttggg aatacatttt agatgtcagc attgcggctg agaattttgc cagttttgat
     7081 acacaactcg ccttcgtcgg acactctcac ctgccgctga tgttcactgc tgaccctcaa
     7141 agtgggcttg tcgaacgcgc ttaccgatac cccggcgtta ccacgcctgt taccacccgc
     7201 gcaatcctca accccggatc ggtcggtcaa ccacgggata atgaccccag agcgtcttac
     7261 ggtatttttg atcctcagaa catgacctgg gaaatccatc gcgttcctta tgatatcccc
     7321 tctgtacaac aacgaatcat cgccgctggt ttaccggaaa agcatgcccg tcgcctgagc
     7381 gctggctggt gagcaggaac caatcctgct cctctggcgt ctattaaaca gttgcacaca
     7441 tcggtttatt tcaaggagga atggtatgaa aacgaaaagc gtgttgattg gcatcgtcat
     7501 tctggcgctc accctcagtg gttgcgcgtc caaatcctac actgctaaca atctgcggtc
     7561 aggcggggtg gaagattacg ccatgaaagc agcccccatg gtagaaccca tggagcccat
     7621 gctggatagt tacgcagaat ctgccggcag ccctggtatc gcagccgcag gggtggaatc
     7681 cgtgcagatg atcatgatga acgccagtct gtcgatcgcg gttgatgacc ctgccgccac
     7741 cctgcgtgac gtgcagcagc tcgccaaagg tatgggtggt tacacagttt cttccaacct
     7801 gtataaaagc tatacgtctt ctggggtcga agtacctcaa gccaacctca ttatcagagt
     7861 gcccgctgag tgcctggacg aagccatgca gaagatcaag gacatgactg gtgaccccaa
     7921 aaaatatact ttgaatgaga gtgtctccgg gcaggatgtg accaaggaat acaccgacct
     7981 gaaatcacgt ttacgcaacc tggaagaagc cgatgccaaa ttgagcgagt tttacgaaaa
     8041 cgccgccaac acagaagatg ccctttccat ctattcgcag aaaatgcagg tgaccgaaca
     8101 aattgaaata atcaaagggc agatcaagta ttacgatgaa tcggtttcta cctcgtccat
     8161 cacattgcag attcaatcca aagaaactat cgctcctatc acggtggctg gttggcagcc
     8221 ttcaggggtt gcgcgcgacg cgcttcaggc gctgctggat ttcggcaaag gtttggtcaa
     8281 cttcctgatc tggctggtga tcctggtggt tcccattgtc ctgttatttg gcctccccat
     8341 cttcttcctg gtgcgctggt tgaaacgccg cagcacagcg cgcaagctgg cagcgctgga
     8401 acgccgcgcg caagagaata aatcagaacc aaccccaccg atcccgacga aaaagtgaat
     8461 ccacgtccac aaataggcaa ggagcggtca acccgctcct ttttctatct cataatacgc
     8521 acattgcaaa aaccttccac gacgactggc agacgataaa ttccttgctc catagggtgc
     8581 aaataaactg gcttgccgtc cacaaacgct gcctgtggct tgcctggcag gtggaaaaac
     8641 acctgcccta ttgccgtcct tggcaggcgc agcgtcatct ccaggctgcc gtccttttcg
     8701 agccactcag ccacctcagc gccttgagtg aaatgcaggt cgctgcccag gtacagcgct
     8761 ttttcgccca caaacctgcg gacagccagt aaaactggcg cgtgcggcgg tatgcctgga
     8821 aatacgtgcc cgcctgtccc ggaaatcatg cctgtcttcc cgctccaaaa ctcgcggaat
     8881 agatattccc cctcttcaag cccatactcg ctggctgaaa tcattacatc cgcggtctgt
     8941 tcagaccagt tgaacccgcc cagcagatgc cagccgccag tctcattgtc catatctaca
     9001 cgaagtctgg acggcgtggc gttgtcgaac caatccaaga cacgcacttc ttcacccata
     9061 actggaatta acgcttctgc caaccgtata cgctcgggtg gcactccagg caggtcatct
     9121 gaaagcagca atgagccgcc agtgagtgcg atgaccgttg ccagtgaacg cacctcttcc
     9181 tgcgtcaggc tcgtgtcagc gcgcacaagc aggcagtctg ggtcatttac ccaccagcgc
     9241 cggttgaggt tggcccgggt aaggatattg tgaatggaat tgcgcgtgct gggcatcgag
     9301 ggttcccggc ggaggcctat ctgcacacct ttgaagaccg gcagccactc ctcgctgaca
     9361 tctggcccaa tgcgcatggt atcgaccacg ccgatgaccg aacccaacgg tgctccgcag
     9421 ccaagcaggg tcgtttgttc gccagctgcc tgccgcaacg cttccatgcc catgcgcatc
     9481 acctgcgccc tggtcttact gcggtcgtag tagtgaccct tcagcgcggc agcatagaga
     9541 aagtccagct tcaaatacgg gaacccccat ttctgcactg ccgtatgaac cacctccgcg
     9601 gtatactgta aagccggcgg tttggtcagg tctaaggcgg tggttaacgc gttccagcct
     9661 aaccctgcat tcaccagcct gccgttggct ttgcgcagca tccaatccgg gtgtttatgc
     9721 agcaggtcag ctccagggtg cacgatgaaa ggcgccagcc acaatcccgg cagcagtcca
     9781 ccgccactga ttgatgcagc cagcggggct accccctctg gaaaggtcga tctgaactcc
     9841 agccagtctc caacctgttt ttcaaacccg tcatcgatct gcaccagttc aatgggcagc
     9901 tcctcctggc gctcaaggat cgtgtccagg ttctcgataa tattcctggc ggtcaccgat
     9961 gtgtaaaaat gataccagga gcaccacccc accggggttt gcgcgggcac gcgcgcgtgg
    10021 ttttcccgcc ctaccgcttc gatataagtc tccagtgggg cgcgatgatc cagcaaaaca
    10081 ggcgtgaaca ccgcccaatc cgtttgcatt gacttccccg cttcaaggca ggcaccatct
    10141 ccattcgccc acatggagat ttcaccggta ttgaagtcag ccaggatcga gccaaagtgg
    10201 ttcaactgag aaaggaaacc gagaaggtaa ccagcacggg tggcgcggtc tccgaccacc
    10261 gcgaacatat cactggagaa aacacctttt ttaacgggcg ttggcgttcc cgggttataa
    10321 atcatcgggc gctgcaatgc gtacagccgg ctgatggtca ttctttgatt cccactgtac
    10381 catcggctgg gagaccagga ttgccagcca ttggagaaaa aacccaggtc atccggccgt
    10441 ctgaccttca ggtaatgggt gttatccacc acgcgcagca gggtgatgcg gtttatctcc
    10501 acatcttctt tcccgtggtt ggtgacctga atcttccaca ataccagcgg gtattctcgc
    10561 ataagcgcga aagacaggcg caggctgacc ttgttggcat ccggctcaag cgcaactgtc
    10621 aggcaatcca tttcgccatg cgcctgcgcg cgctccaggt gatgcgccaa gccattcacc
    10681 tgccagccat cttgaccgca gcgctgctgc ttccccgcga acgtgtactc gcagcccagt
    10741 tgaatattct ccaagcgggg aaaacgaggg tccctggcct ggatcgagaa agtccctgaa
    10801 tcagggttga cctgaagcat taatgaaaca gaattgagct gctccaatta aatcctccac
    10861 acaggcaatg aataaagtaa tcttatcacg aggctctaaa acccgcttcc agtcccctta
    10921 aaacacgcgt gatataattg ccgcatgccc cccatggtcg ccattgatat cgaaacaacc
    10981 ggaatggacg cgcagcgtga cgcgatcatt gaaattgccg ctgtgcgttt taatgggcac
    11041 agggtggaag gagaatggac gaagctgatc aaccccggcc gccccattcc ccggcaaatc
    11101 acgcaactca ccggcatcac tgaccaaatg gtgctgcacg cgccaccact gcaagcggtc
    11161 attcaggaac tggtggattt tgtaggcaac gacatcgtgg tcggtcacaa tgtgcgtttc
    11221 gacctgagtt ttttacaaag gcagaaggtc ctcctttaca acaaagtgat cgacacctac
    11281 gagatggccg ctattgcgct gcccggtaac agccgttaca acctcggcac cctcggggtt
    11341 acctgcaaca ttctgattcc caactcgcac cgcgccctgg acgatgcccg gctggcgcag
    11401 gcagtctatt tttcactctc acaaaagttg ctggaattac ccattgaaat ccttgatgag
    11461 atcgtgcgca tgtcagaagg cattgaatgg gatggcgagg ttgcttttca cctggctttg
    11521 caggcaaaag gccgtttacc cattgaagcc cggaaggctt ctcaagacga tttcggcttg
    11581 ctttttgcag ga
//
LOCUS       DAOTLW010000048        11297 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_6118_length_11297_cov_11.361679, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000048 DAOTLW010000000
VERSION     DAOTLW010000048.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 11297)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 11297)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11297
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6118_length_11297_cov_11.361679"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..123
                     /locus_tag="P5516_07365"
     tRNA            48..123
                     /locus_tag="P5516_07365"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:81..83,aa:Phe,seq:gaa)
     gene            197..1081
                     /locus_tag="P5516_07370"
     CDS             197..1081
                     /locus_tag="P5516_07370"
                     /inference="COORDINATES: protein motif:HMM:NF022834.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2877 domain-containing protein"
                     /protein_id="HRT92290.1"
                     /translation="METIKVRLIGAAAQESLMKAAARRWVAGVTSKGAFLRTDDRVLF
                     LTNSDYRSPFNLTLNSIGQLFTRLQPGDTFETNKNEIVFSGREITLLTHGAAVWHPPQ
                     PARMVASLQEQRLRANLLLDTIKSIDPDKGFLFLAGQPDDQTEVFQREIHHAARQIVL
                     AYRSQNKEGFFASAEKILGAGGGLTPSGDDFLTGFLLYHYRYLQSVNSEPGLLNEWWQ
                     ELTRLAFKKTTTISANRLEYAGRGWAEEIFLTLIDHLFTPEVAFGIEKARHLVDFGHS
                     SGVDTLIGISYGLDSLLV"
     gene            complement(1141..1866)
                     /locus_tag="P5516_07375"
     CDS             complement(1141..1866)
                     /locus_tag="P5516_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92291.1"
                     /translation="MKFELPEHASQEVLEIDVREIEALLQGPAMDLPKWPGAQVKLKD
                     GRTLYIREARREEAPRMMEYMKRLMDVDHDFYDIVGARVYSEILGWYRHRLKDPYTLV
                     GLIDGVWAGFANGRVMNEKVNVSLHTMALLRGGRIGAVMYYAKAYYAFEMIGNTEWWA
                     TYESYNGWLRWGLGMAQPSYPWPEVQHELGGARVYYITKKYWDQLVKAYVQQMAGADL
                     DFNVPDAVRQANEEMIVPEEILV"
     gene            complement(1890..2771)
                     /locus_tag="P5516_07380"
     CDS             complement(1890..2771)
                     /locus_tag="P5516_07380"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HRT92292.1"
                     /translation="MKLLDLTIPIGIGTPPWPTYEPLQVKYFKRLAPNGANGQVLTHS
                     NHLGTHLDGEIHFYTPGRDIAQLPMDFLVHEAAIVDLSDVCGDYDVYTSKMITDRVEV
                     KEGDILVIHTGYHHFGWDQPTADEVRYMTKHPGPDHEFVDWAIKMKLRWIAVDCGSAD
                     HPMNTIIRTWMPRQAKQAEYVFKKKFGKTLEEFFTDDKYQMMHIDLFPKEIIHAECFG
                     GMIDLLLNRRVEVGFFPWRFVDGEASIGRAVAFVDDKEYDELMKKAATMPKTKFGDVY
                     DPKHVESLNRLSKANLA"
     gene            complement(2907..4052)
                     /locus_tag="P5516_07385"
     CDS             complement(2907..4052)
                     /locus_tag="P5516_07385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012677963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium ion-translocating decarboxylase subunit
                     beta"
                     /protein_id="HRT92293.1"
                     /translation="MGFDLSGLLGGLLDITWQQLIMIAVALLLLYLGIFKDYEPTLLI
                     PIGFGCILGNLPVAANMIGEEGLFGVLYRAGISNELFPLLIFLGVGAMLDLRPLLSQP
                     VMFVMGALAHLGIFATLVIAILLGFNLAEATAIGMIGAIDGPTVIYVNSKLGSLFVTP
                     GLAAAIAVTAYSYMSLVPIIQPPLMRLFTTKKERAIRMEYTPRPVSRMTVILFPILIT
                     AVAGIFLPEAAPLIATLMLGNLIKESEVVPGLVETSKNAITNTATIFLGITIGSTMQA
                     ATFLNTGTLRVLILGLIAFALNTIGGLLFGKLICLLSGRKINPLVGAAAISAFPMSGR
                     LAQKVALEVDNQNFILMHALGANAAGQVASVLAAGILMQMIFPILLH"
     gene            complement(4070..4507)
                     /locus_tag="P5516_07390"
     CDS             complement(4070..4507)
                     /locus_tag="P5516_07390"
                     /inference="COORDINATES: protein motif:HMM:NF012584.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92294.1"
                     /translation="MTQYKITVKDTTYDVEILDDPRNDEVLVKVNGEELTVVTEEVNK
                     AVSAVKAAKPAPARAAAPAPKPAAAAPVAAGPGTIKSPLPGVVNSIKVRAGDKVKAND
                     EICVIEAMKAMNIIRAPQDGTVARIHVNEGNQVAYGAPLMDIE"
     gene            complement(4504..4860)
                     /locus_tag="P5516_07395"
     CDS             complement(4504..4860)
                     /locus_tag="P5516_07395"
                     /inference="COORDINATES: protein motif:HMM:NF016187.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008948 - oxaloacetate decarboxylase
                     activity [Evidence IEA];
                     GO_function: GO:0015081 - sodium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0036376 - sodium ion export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OadG family transporter subunit"
                     /protein_id="HRT92295.1"
                     /translation="MENLGLALKISGIGIVVLLLVLAILAGIIYLLTLVLVDKEDEEE
                     SKDTVVDATTEPEKEKADSKSHALLAAALAVAIARAKAESVVSFADASTGEVNPWRQF
                     GLQRRLTQSSTIRRTR"
     gene            complement(4878..6440)
                     /locus_tag="P5516_07400"
     CDS             complement(4878..6440)
                     /locus_tag="P5516_07400"
                     /EC_number="6.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016874 - ligase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HRT92296.1"
                     /translation="MAQDSTDKIAKLKALREESRLGGGEKRIAAQHEKGKKTARERID
                     ALLDKGSFNEINGLMKNRHTDFGLDKDKIPGDGLVAGFGTINGRKVCVYAQDFTVMGG
                     SFGEVAGEKVARVMDLAMDAGVPVIGINDGGGARIQEGIYSLYAYGEVFYRNVQASGV
                     VPQISVIMGPCAGGGVYSPALQDFIIMTQGMGNMYITGPEVIKAVTNEIVDHETLGGA
                     VTHTTVSGVAHFLGQDEDDTFHIIRKILEYIPDNNASTPPVLPTNDSPFRADKELNTL
                     VPADPNQTYDIRDVINRVVDIDSFFEVHHNFATNAVVGFARLNGESVGIAANQPASMA
                     GVLDINSSDKIARFVRFCDAFNIPVITFVDTPGFMPGTHTEHNGIIRHGAKILFAFSE
                     ASVPKISVVTRKAYGGAYIVMGSKHLRADMSFAWPGAEIAVMGAGGAVNILHGRSLKD
                     KDPEEAKKLRAELVADYEDKFSNPYQAASAGYIDEVIIPSETRQRLIATLDILRNKQS
                     TSPAKKHGNIPL"
     gene            complement(6628..7539)
                     /locus_tag="P5516_07405"
     CDS             complement(6628..7539)
                     /locus_tag="P5516_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92297.1"
                     /translation="MGDFLVDLIAATLRIATPLIFATLGELFCERAGILNLGVEGTMF
                     FGAFSGFTAASLTGSLWMGILCAFIGGMISGLIMSLFSVRLGVNQHVSGLGVTLLMTA
                     LSLFGFRVIFGENRILPSIQPFQQLSIFSGSRVLGPIFSQYTLTYIALALIPIVWWVV
                     FRTNFGLNLRSVGDNPEATDAAGINVYRLRTIALVIGGALMSVGGAYLSVAQLGSFTF
                     GIVAGRGWVCIALIIFANWHPVRVLWGALLFGGVQALQSRLQLTGLKLPYELFLALPY
                     IVTIIALTIAGRNASYPAALLKPYKRE"
     gene            complement(7542..8594)
                     /locus_tag="P5516_07410"
     CDS             complement(7542..8594)
                     /locus_tag="P5516_07410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92298.1"
                     /translation="MIKFRLERSPAPVWTKALIPVVAVLVTFAITSIFILLAGANPFA
                     AYYNFIIVPLSTKFSALEVLVKATPLIFTGIAVTFAFAAGYYNIGAEGQLYAGAVAAA
                     WVGATFTTLPSIVVIPMMLVLGFLSGMLWALIPALLKARLKVDEVVTTLLMNSIIMYF
                     ISALLNGPWRDPETGWPKSPEFFEAARFMQLIPKSRLHIGFIIALVVVVVVYIIIKRT
                     PFGLQMRAVGASKAGAQFAGINVNRTMLIAALVSGGIAGLAGVSEIAGIHYHLISELS
                     GGYGYTGIIVATLGGLNPIGATIAALFIGLINTGAQTVSRVLGVPIYLGNVVQSTLLL
                     VTLGMLLLQNYRIKRI"
     gene            complement(8623..10107)
                     /locus_tag="P5516_07415"
     CDS             complement(8623..10107)
                     /locus_tag="P5516_07415"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92299.1"
                     /translation="MLAVEMKHINKTFGPVKANQDVSFELEQGEIHALLGENGAGKTT
                     LMRILYGLYRADSGEIVINGKKVTIHSPKDAIAAGIGMVTQHFTLVPTLTVAENIVLG
                     YSNKMVLSQKEIEKDVAQASEKYGIPVDPSTLVKNLSVGERQRVEILKALYRNARILI
                     MDEPTAVLVPQEVDVLFESLVRLVKSGLSVIFISHKLVEVMTVCDRITVLRDGKVANT
                     VKKSETTQVDLAKMMVGRETFGVQRQSAREASEPVYEVTEITLEGKHGRKVLDKVTFN
                     VGVGEILGIAGVSGNGQGELTEVLNGLVKPSSGTIKINGKDITCCSPSAISDCGIGRT
                     PQDRHEGTIGELTVAENLALEHISNYHRKGMLDRKKIQEDAAQTIQEFQIKASPGDQL
                     RKLSGGNMQKVVLARALSRKPKVLIASQPTRGLDVGATEYVRNRLLKERERGTAVVLL
                     SEDLDEVLALSDRIAVIYEGSIRGIIPAVEATPEKLGLLMAGAA"
     gene            complement(10188..11249)
                     /locus_tag="P5516_07420"
     CDS             complement(10188..11249)
                     /locus_tag="P5516_07420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family protein"
                     /protein_id="HRT92300.1"
                     /translation="MFKKSYLLVFVAILMVVSLMAGCAKPPVAPTTAPAEVEAPEAPA
                     AEESGVIKVYGAFATPIEEPWDGVIHDALLKAKEEGLIEYTYTEDIGYSGDMERVLRE
                     IAEQQKPQLIMGDAFVNEEAVRRVAADYPEIAFAFGSELGPVAPNLAVFDNWIHEPAY
                     LCGMLAGGLTKTNTIGVVAAVPIPEVNRLVNAFIAGAKETNPEVKVLVSFINSFFDPA
                     TAKEQALAHISAGADVLFAERAGVIEAAAEKGVFVFGNMSDQNEIAPEWVISGPVWNM
                     TPTVEYVVKQVKAGTFTAMDLKDFNMYALGGATLAPFHGNESKIPADVLAAVTAKEEA
                     IKSGMFRVPIDEAQPAAVN"
BASE COUNT         2656 a         2908 c         3050 g         2683 t
ORIGIN      
        1 gaatttcttg acaatccttg gagcgtcggg tagaattggc cttacctgcc gagatagctc
       61 agttggtaga gcacacgact gaaaattgtg gggtcgccag tccgatcctg gctctcggca
      121 ccatacactc cctcaaggga gtgtaatttt ataccgtggt ttgatggatt ggttcaagaa
      181 cggagggtgt taaggagtgg aaacgatcaa ggtacgcttg ataggagccg cagcgcagga
      241 atccctgatg aaggcagcgg ctcgcaggtg ggtagccggg gtgacctcca agggggcttt
      301 tctgcgcact gatgacaggg tgcttttcct gactaattca gattaccgca gcccgtttaa
      361 cctcactctt aactcgattg ggcaactttt tacccggctg cagccaggtg acacgtttga
      421 aacgaacaag aatgagatcg tgttcagtgg gcgggagatt acgctgctca cccacggggc
      481 ggctgtgtgg catccacctc aacccgcgag gatggtggct tctctgcagg aacagcgact
      541 acgtgccaat ttattgctcg acacgatcaa aagcatcgat cctgacaagg ggtttctctt
      601 tctggcaggg cagcctgacg accagacgga ggtctttcag cgcgaaattc atcacgcggc
      661 tcgccagata gtgctggctt atcgaagtca aaataaagag ggttttttcg cttcggctga
      721 aaaaatcctt ggcgcaggcg gcgggttaac cccatcaggc gatgatttcc tcaccggctt
      781 tctgctgtac cattaccgct acctgcaatc cgtcaactct gagccaggat tattgaatga
      841 atggtggcaa gaacttaccc ggctggcatt taagaaaaca accacgatct cagccaaccg
      901 gttggaatac gcagggcgtg gttgggcgga agaaatattt ttaacgttga tagaccacct
      961 tttcaccccc gaggtggcct ttgggattga aaaggcaaga catctggtgg atttcggtca
     1021 ctcttcaggg gttgataccc tcattggtat ctcgtacggg ctggattcgc tgctcgttta
     1081 gtctgagaat aaaaacagga gccagattcg gctcctgttt tttcgcgtaa tgcaagtctg
     1141 tcagaccagg atctcttcag gaacgatcat ttcctcgttt gcctgtcgaa cagcgtcagg
     1201 cacattgaaa tccaggtctg cgccagccat ctgctgaaca tatgccttga ccagctgatc
     1261 ccagtacttt ttggtgatgt agtaaacacg cgcgccaccc agctcatgct ggacttccgg
     1321 ccagggataa gaaggttgtg ccataccgag accccaacgc agccatccgt tgtaggattc
     1381 gtaagtcgcc caccactcgg tgttaccaat catttcgaac gcgtaatacg ccttggcgta
     1441 gtacatcacc gcgccgatgc gtccgccgcg cagcaaagcc atggtgtgca ggctgacgtt
     1501 caccttttca ttcatcaccc gaccattggc aaagccagcc caaaccccat caatgagacc
     1561 aaccagagta taggggtctt taaggcgatg acgataccag ccaaggatct ctgaatagac
     1621 acgggcgccg acgatgtcgt agaaatcatg gtccacgtcc atcaggcgct tcatgtattc
     1681 catcatcctg ggggcttctt cacggcgggc ttcgcggata tagagggtgc ggccgtcttt
     1741 caatttgacc tgggcgcctg gccacttggg cagatccata gccgggccct gcagcaaggc
     1801 ttcgatctcg cgaacatcga tctctaatac ctcctggctc gcgtgttcgg gaagttcgaa
     1861 tttcattctg gtctcctaac cggcaaatgc taggcgaggt tggctttgct gagtctgttc
     1921 aggctttcaa catgcttggg atcatagacg tcaccgaatt tggtcttggg catggtggca
     1981 gctttcttca tcagttcatc gtattctttg tcatcgacga atgccaccgc gcgtccaatg
     2041 gaagcctcgc catcgacaaa gcgccagggg aagaaaccaa cctccacacg gcggttgagc
     2101 agcaggtcga tcatgccgcc aaaacattcc gcgtgaatga tttctttggg gaacaggtcg
     2161 atgtgcatca tctggtactt atcatcggta aagaactctt caagggtctt gccgaatttt
     2221 ttcttgaaca cgtattcagc ctgtttcgcc tgacgcggca tccaggtgcg gatgatggtg
     2281 ttcatcgggt gatcagcaga cccgcagtcc acagcaatcc agcgtaattt catcttgatc
     2341 gcccaatcga cgaattcgtg gtcaggaccc gggtgtttgg tcatatagcg cacttcatca
     2401 gcggtgggtt gatcccagcc gaaatgatgg taaccggtgt ggatgacgag gatatcgcct
     2461 tctttcactt ccacacggtc ggtgatcatt ttgctggtgt aaacatcata atcaccgcag
     2521 acgtcgctca ggtctacaat ggctgcttcg tgcaccaaga aatccatggg cagctgggcg
     2581 atgtcacggc cgggtgtgta gaaatggatc tctccgtcaa ggtgggtgcc gaggtggttg
     2641 gagtgggtga gcacctgccc gttggcgcca ttcggagcca ggcgtttgaa gtacttcacc
     2701 tgtaagggtt cataagtcgg ccagggtgga gtaccaattc cgatggggat ggtcaaatca
     2761 agtagtttca tgatacgctc cttttttgaa tttttttcaa cacttatggt gttgaaattg
     2821 aaaacagtca tggggaatta aggatcattc tccatgactg ttcttcttac aagtgggtga
     2881 gatacctgtt gaagtgcata ttctcactag tgcagcaaga tcgggaagat catctgcatg
     2941 aggatgccgg ctgccagaac agaagcaacc tgcccggctg cgttggcgcc aagcgcgtgc
     3001 atgaggatga agttctgatt atcaacctca agcgcgactt tctgcgccag gcgaccgctc
     3061 atcgggaagg cgctgatggc ggctgcgcca acgagcgggt tgatcttgcg cccggaaagg
     3121 aggcaaatca gttttccgaa gagtagacca ccgatggtgt tgagggcgaa agcaatcaag
     3181 cccaagataa gaacccgcag ggtgcccgtg ttcaagaaag tggctgcctg catggttgag
     3241 ccgatggtga tacccaggaa gattgtggct gtattggtga tggcgttctt agaggtctcc
     3301 accaaccctg gcacaacttc tgattctttg atgaggttgc caagcatcag ggtggcgatg
     3361 agtggcgcgg cttctggcag gaagatgccg gcgaccgcag taatgaggat ggggaaaagg
     3421 atgaccgtca ttctggaaac cggtcttggg gtgtattcca tgcggattgc gcgttctttt
     3481 ttggtggtga agaggcgcat cagaggaggc tggatgatag gcacaaggct catataggaa
     3541 tacgccgtga ccgcaattgc ggctgcaaga ccgggggtta caaacagcga gcctaactta
     3601 ctgttgacgt aaatgacggt tggaccatcg atggcgccga tcatgccaat ggcggtggct
     3661 tcagcgaggt tgaagcccag caggatggca atgaccaacg tggcaaaaat tcccaggtga
     3721 gccagcgcgc ccatcacgaa cataacaggc tggctgagca gcgggcgcag gtcgagcatc
     3781 gccccaacac ccaggaaaat caacagggga aagagttcgt tactgatccc tgctcggtag
     3841 aggactccaa ataagccttc ctcaccgatc atgttggcag caacgggcag gttccccagg
     3901 atacacccga aaccaatcgg tatcaatagc gtgggctcat aatccttaaa aatcccgagg
     3961 tacaacagga gtagagccac agcaatcatg atgagctgct gccatgtgat atcgagaagt
     4021 cctccgagca aacctgataa atcgaagccc atgatcgtgt cctttccgcc tactcgatat
     4081 ccatgagcgg agcaccataa gcaacctggt tgccctcatt cacatggatg cgagcgactg
     4141 taccatcctg gggagcgcgg atgatattca tggctttcat ggcttcaatg acacaaatct
     4201 cgtcattggc tttaacctta tcgcctgcgc ggactttaat cgagttgacc acaccgggaa
     4261 gaggtgattt gattgtgcca ggaccggcag ccacaggagc agccgctgcg ggtttaggcg
     4321 caggcgcggc cgcacgggcg ggcgcgggtt tggcggcttt gacagctgaa accgctttgt
     4381 tgacttcttc agtgacaact gtcagttcct ctccattgac ttttactaaa acttcatcat
     4441 tgcgtgggtc atcaaggatc tcaacatcgt aggtggtgtc cttgacggta atcttgtatt
     4501 gtgtcatctt gttctcctga ttgtggaaga ttgtgttagc cggcgctgaa gcccaaactg
     4561 gcgccaggga ttgacttctc cggtggaagc atctgcgaat gaaacaaccg attcagcttt
     4621 tgcccgcgca atcgccactg ccagggcggc tgccagtaag gcatgcgatt tcgaatccgc
     4681 tttttctttc tccggttcag ttgttgcgtc tactacggtg tctttgcttt cttcctcatc
     4741 ttccttgtca accagaacca aggtaagcag gtagatgatc cctgccagga tagcgaggac
     4801 aagtaggagc accacgatgc cgattccgga tatttttaac gctagtccga ggttttccat
     4861 tatccatatc ctttctgtta cagcgggatg ttcccgtgtt tcttggcagg agaagtggac
     4921 tgcttgttcc ttaagatatc gagagtggcg ataagacgct ggcgggtttc actggggatg
     4981 atcacctcgt caatataacc agcagaagca gcctggtaag gattggagaa tttatcctcg
     5041 taatctgcta ccaattcagc gcgcaatttc ttggcttctt cgggatcttt atccttgagc
     5101 gatcttccgt gaaggatatt cactgcaccg cccgcgccca tcaccgcgat ttccgcgcca
     5161 ggccaggcaa agctcatgtc agcgcgcagg tgcttgctgc ccataacgat gtaggcgccg
     5221 ccgtatgctt tgcgggtgac aacagagatc ttgggtactg aagcctctga gaaggcgaac
     5281 aggatttttg cgccgtggcg aataatcccg ttgtgctctg tgtgtgtacc gggcatgaac
     5341 cccggggtat ccacaaaggt gatcacgggg atattgaagg cgtcgcagaa acgcacgaaa
     5401 cgggcaatct tgtcagagga gttgatatcc agaacgcccg ccatagaagc aggttgattt
     5461 gcagcgattc ccacgctttc accattcaag cgggcaaaac caaccaccgc gttggtggcg
     5521 aagttatggt gaacttcaaa gaacgaatcg atatccacta cgcgattgat cacgtccctg
     5581 atgtcgtaag tttggttggg gtcagcgggt accagggtgt tcaattcttt gtcggcgcgg
     5641 aatgggctat cattggttgg gagaacaggg ggagtgctgg cgttgttatc ggggatgtac
     5701 tcgaggattt tgcggatgat atggaaggtg tcatcctcat cctgtcccag gaagtgcgcg
     5761 acacccgaaa cggtggtgtg ggtgacggct ccgcccaatg tttcgtggtc cacgatttca
     5821 ttggtaaccg ctttgatcac ttccggtccg gtgatgtaca tgttccccat gccctgggtc
     5881 atgatgataa agtcctgcaa ggcgggagag taaacgccgc cgccggcgca gggtcccata
     5941 ataacgctga tctgcggcac cacacccgaa gcctgaacgt tgcggtagaa tacctcgcca
     6001 taggcataca ggctgtagat gccttcttga atgcgcgcgc cgccgccgtc attgatgcca
     6061 atcacgggca cgccggcgtc catcgccagg tccatcacac gggcaacctt ctcaccagcg
     6121 acttcgccaa aagagccgcc cattacggtg aaatcttgcg cgtaaacgca gacttttctg
     6181 ccgttgatgg tgccaaagcc agcgacaaga ccgtcaccag ggatcttgtc tttgtctaaa
     6241 ccgaaatcgg tatggcgatt tttcatcaag ccattgattt cgttgaagga tcctttgtct
     6301 aagagggcgt cgatacgctc acgggctgtc ttcttgcctt tttcgtgctg agctgcgatc
     6361 cttttttcgc cgccgcctag ccggctctct tcgcgaaggg ccttcaattt ggctatcttg
     6421 tctgttgaat cttgcgccat tactacctcc atctttgaat tttgatgttt taagaacaat
     6481 tatagtttat gcttatccat ctggcattac aagtgataca tttagcatcc tgaacggttg
     6541 attgtcacat aaatcttaca gcaaagaata aaaaaagcat gtgaccgagt tgaatcacat
     6601 gctttttgaa ctgagtagaa aaaattctca ctctctttta tagggtttga gtaatgccgc
     6661 ggggtaagat gcgttgcgac cggcgatggt gagcgcgatg atggtgacga tgtatggcag
     6721 ggcgagaaag agctcgtaag ggagcttgag accggtcaac tgcagcctgg attgcagcgc
     6781 ctgcactccc ccaaacagaa gcgcgcccca caacaccctt accgggtgcc agttagcgaa
     6841 gatgatgagg gcgatgcata cccagccgcg tccagcgaca ataccgaagg tgaacgagcc
     6901 aagttgtgcc actgaaaggt aagcaccacc cacgctcata agcgcgccgc cgatgaccag
     6961 ggcgatggtg cgcaggcgat acacattaat gccagccgcg tcagtcgctt ctgggttatc
     7021 tcccactgaa cgcaggttca gcccaaaatt cgtacggaag accacccacc aaacgatggg
     7081 aataagagcc agggcgatgt aggtgagggt atactgcgag aaaattggac ccagcacacg
     7141 gcttccggag aagatactga gttgttggaa aggctgaatc gacggcagaa tacggttttc
     7201 gccgaaaatg acccggaacc caaataacga gagcgcagtc attaagaggg tcacccctaa
     7261 accagagacg tgctggttca cccccaatct tacggagaac aggctcatga tcagaccgga
     7321 gatcattcca ccaataaagg cgcaaagaat gcccatccat aaagaaccgg tcaacgacgc
     7381 ggcggtgaac ccactaaacg cgccaaaaaa catggttcct tcaacgccaa ggtttaaaat
     7441 tcctgcacgt tcgcagaaaa gctctccgag cgtggcgaag ataagcggtg ttgcgatacg
     7501 cagcgtggct gcgataagat cgaccagaaa gtctcccatg atcatatcct cttaatccga
     7561 taattctgaa gcagcagcat gcccagggtg acaagcaaaa gcgtagattg gaccacattt
     7621 cccaggtaaa taggtacgcc taacacgcgg ctcaccgttt gcgcgccggt gttgatcaac
     7681 ccgataaaca gcgccgcgat ggtagcgcca attgggttca atccgccgag ggtggctacg
     7741 atgataccgg tgtagccata gccgcctgat agttcgctga tgaggtggta atgaataccg
     7801 gcaatttcgc tcacgccagc caacccggcg ataccgccgc tgaccaaagc agcgatgagc
     7861 atggtgcggt ttacgttgat accagcgaac tgcgctccag ccttactggc gccaacagcg
     7921 cgcatttgca gcccaaatgg tgtgcgcttg atgatgatgt aaacgaccac caccaccacc
     7981 agagcgatga taaaaccgat gtgcaggcgt gacttgggaa taagctgcat aaaccttgca
     8041 gcctcgaaga actctggtga ttttggccat cccgtttcag gatcgcgcca gggaccgtta
     8101 agcagcgcgg agatgaaata catgatgatg gaattcatca gcagggttgt caccacctca
     8161 tccactttca acctggcttt cagcaatgcc gggatgagcg cccaaagcat gccactgaga
     8221 aaacccagca ccagcatcat ggggatgacc acaatagagg ggagggtggt gaatgtggcg
     8281 cctacccagg cagccgcaac cgcgccagcg taaagttgac cttccgcgcc gatgttatag
     8341 taaccggcgg cgaaagcaaa agtaaccgcg atacctgtaa agatgagagg agtggctttt
     8401 acgagcactt caagggcaga gaacttggtg gaaagaggga cgatgataaa gttgtaatat
     8461 gcggcaaatg gattcgcccc cgccaggagt ataaaaatgg atgtaatagc gaatgttaca
     8521 agcaccgcta ccaccgggat gagcgctttg gtccaaaccg gagcgggaga tcgttcaagt
     8581 cggaatttga tcacaataaa tcctccaatg cgagaaataa gcctaagcgg cgccggccat
     8641 cagcagcccc agcttctcag gggtggcttc aaccgccggg atgatacccc ggatactgcc
     8701 ttcatagatc actgcgatgc ggtcagacaa cgccagtact tcgtccaggt cttctgagag
     8761 caagaccacc gcagtaccgc gttctctttc tttgagcagc ctgttgcgta catattcggt
     8821 cgcgccgacg tccaacccgc gggtgggttg tgaagcaatg agcacttttg gtttacgcga
     8881 taaagcgcgc gccagcacaa ctttttgcat attcccgccg gataacttgc gcaactggtc
     8941 gcctggagag gctttgatct ggaattcctg aatggtctgc gcagcgtctt cctggatttt
     9001 tttccgatcc aacatccctt tacgatggta attggaaata tgctccagag caaggttttc
     9061 tgccacggtg agttcaccga tggtgccttc gtgtctgtct tgcggcgtgc gaccgatgcc
     9121 acaatcagaa atggcgctcg gtgagcagca ggtgatgtct ttcccattga tttttattgt
     9181 gccgctgctg ggttttacaa gaccgttaag cacctcagta agctctccct gtccgttacc
     9241 tgatactccg gcgataccga gaatctcgcc tacaccgacg ttaaaagtga ctttatccag
     9301 cactttgcgc ccgtgttttc cttcgagggt gatttcagtg acttcataga caggttcgct
     9361 cgcttcacgt gcagactggc gctggacgcc gaaagtttca cgaccaacca tcatttttgc
     9421 caggtcaacc tgggtggttt cggatttttt aaccgtattg gcgaccttgc cgtcgcgcag
     9481 cacggtgatt ctgtcgcaaa cagtcatcac ttcgacgagt ttatgactga taaatatcac
     9541 ggaaaggcca gatttcacca atctgacgag ggattcgaac agaacatcaa cttcctgtgg
     9601 caccagcaca gccgtcggtt catccataat gaggatgcgc gcgttgcggt ataacgcttt
     9661 gagaatttcc acgcgctgtc tttctccaac tgacaggttc ttcaccaggg tggaagggtc
     9721 caccgggatg ccatattttt cagaagcctg ggcaacatcc ttctcgattt ctttttgaga
     9781 aagaaccatc ttgtttgaat aacccaggac aatgttttca gcaacagtca gggtgggcac
     9841 gagagtgaaa tgttgggtaa ccatgccaat gccggcggca atggcgtctt taggtgaatg
     9901 aatggttact tttttaccat taatgacgat ttcaccgcta tcggctctgt ataaaccata
     9961 gagaatacgc atcaaggttg ttttaccggc gccgttttct cctaacaggg cgtgaatctc
    10021 accctgctcc aattcgaaac tcacgtcttg attggctttt accgggccga aggttttatt
    10081 tatgtgcttc atttcaacag ctaacatggc aaatccttca tcgtagattt cggtacggat
    10141 ttaaaggggg gcggcagagt taccttctgc cgcccctgat tttcggatta atttacagcg
    10201 gctggttgtg cttcgtcgat gggaacacgg aacataccag acttgatggc ttcttctttg
    10261 gcagtgaccg cggcgagcac atcggccggg atcttgcttt cattgccgtg gaatggtgcc
    10321 aaggtcgcgc cgcccaacgc atacatgttg aagtccttca ggtccatggc ggtgaaagtg
    10381 ccagccttga cctgcttgac cacgtattca acggtggggg tcatattcca gactggaccg
    10441 ctgatgaccc attcgggggc gatttcgttc tggtcgctca tgttgccgaa cacgaagaca
    10501 cccttttcgg cggccgcttc gatcactcca gcgcgctcag caaagagcac atccgcgcca
    10561 gcgctgatgt gcgccagggc ctgctctttg gcagtggcag gatcaaagaa cgagttaatg
    10621 aaggaaacga gaaccttaac ctctgggttg gtttccttgg ctcccgcgat gaaagcattc
    10681 accagacggt taacttcggg gatgggcaca gccgcgacca caccgatggt gttggtcttg
    10741 gtcaacccgc cggcgagcat accgcacagg taagccggtt cgtggatcca gttatcaaat
    10801 actgccaggt ttggagcgac cgggccaagc tcagaaccga aagcaaaggc gatctcggga
    10861 taatcggcag ccacacggcg cacggcttct tcgttcacga acgcgtcacc catgatcaac
    10921 tggggtttct gctgttctgc gatttcgcgc agcacgcgtt ccatatcgcc tgagtagccg
    10981 atatcttcag tgtaggtgta ctcgataagg ccctcttcct tggctttgag cagcgcgtca
    11041 tggatgacac catcccatgg ctcttcaatg ggggtggcaa acgcgccata gaccttgatt
    11101 accccggatt cttcagctgc gggggcttca ggtgcttcga cttcagcggg ggcggtcgtt
    11161 ggcgcaacgg gcggcttggc gcaaccagcc atcaggctga cgaccatcag aattgcgacg
    11221 aacacaagca agtatgactt tttaaacatt tttttctcct tctttgctag ttttaaatac
    11281 acttgcgttt tcaacta
//
LOCUS       DAOTLW010000049        10719 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_6470_length_10719_cov_9.643661, whole genome shotgun sequence.
ACCESSION   DAOTLW010000049 DAOTLW010000000
VERSION     DAOTLW010000049.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10719)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10719)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10719
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6470_length_10719_cov_9.643661"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1100)
                     /locus_tag="P5516_07425"
     CDS             complement(<1..1100)
                     /locus_tag="P5516_07425"
                     /inference="COORDINATES: protein motif:HMM:NF028199.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HRT92301.1"
                     /translation="MNQLRKLFLLIKELGVADLVSYSLYQAQLRSGYTRKLTPLGGLP
                     APEQFLVSAAPEFCWRSCWERLSGKFSARLMIEEARLLKDGYYRPFYATSQILHLEPP
                     SQSSVHWIDVNEERFDDIKMVWEPARFFWSLTLAGVYRGKAQESAAELFWQKFDEFNL
                     NNTVNAGPNWVSAQEVGMRAMMWIIAISAFHSSSFSTSQRLERLTTAIVHHVERILPT
                     LNYARSQHNNHILSESLCLMLAGDFLANLHPRAREWSRQGESYFNHALLRQVEEDGTY
                     AQHSANYHRLMLQLSLLYHARMRQQNRELPPAVMRKLAAASRWMLAQLDPLSGRLPNL
                     GHNDGTLLLPFGCDEFRDYRPTAQAAAIAFLG"
     gene            complement(1126..3276)
                     /locus_tag="P5516_07430"
     CDS             complement(1126..3276)
                     /locus_tag="P5516_07430"
                     /inference="COORDINATES: protein motif:HMM:NF013567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bi-domain-containing oxidoreductase"
                     /protein_id="HRT92302.1"
                     /translation="MKQILQYLNNGKTVVEEVPVPAVRRGMVLVQTACSLVSAGTERM
                     VVDFASKNLVGKATSRPDLVKQVVQKAMREGLFPTVEAAFNKLDQPMPLGYSSSGIVV
                     KAGEDALDFKAGDRVACAGGGYAVHAEYALVPVNLAARLPQNVDFESAAFTTLGAIAL
                     HGFHLASPQLGESVCVIGLGLLGLLSAQIARAAGCLVFGIDLSEERVSLAQALGFSAA
                     QRQQCANLAAGVTNGLGFDHVLICADTPSQDPVELAGDIARDRGTVVAVGAVGMQIPR
                     KRYYEKELVFKISRSYGPGRYDPVYEEGGIDYPPGYVRWTEGRNLQAFIDLMHTGKVD
                     VRPLISHRIPIEDAVSAYDLISGKRSEPFMGVLLTYPVKEDFSNQQKVLVTPSAANVP
                     AAIKLGVLGAGNYAQAVFLPVIKKTPGAQLVGIATASGMSAQQAARKFGFEFATTSEE
                     EILDNADINTVVLLTRHQHHARQVLSALRHHKAVYCEKPLALNAEELSEIEAELARAD
                     APLLSVGYNRRFAPLTLTLKNFIAQRTEPLYAHYRVNAGFLPLNHWLHDPEQGGGRII
                     GEACHFIDLLTFLIGAVPVSVSAAALADQAKYRQDNVQLTFRYADGSIGTVAYLANGN
                     KNFPKERIEVFSSGKIGVLEDFRSLALVSENERKTSRSVLRQDKGHAQSWAAFLAAVK
                     KGGTPPIPYDELLNTSRASFAALQALRTATEMKV"
     gene            3412..4077
                     /locus_tag="P5516_07435"
     CDS             3412..4077
                     /locus_tag="P5516_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92303.1"
                     /translation="MLKKILWILMLLIMTACTILFKDGVNIVLGELTPTMVATSTPVD
                     IGVEITPTETVEPTMEPTATEEPVNLPSPTIVTTAEPEPTPTPEATATPFVAVYEVQK
                     GSPAYLDNFAHLSDGCAWQGIAGQVFNSKKEPVTNLVVKVSGEWNGKKIASVGVTGMV
                     AGLPYGPGSYEIVLGDKAVNSNATLYVQLFDEGQKPLTEPNAVSTQADCQKNLVILNF
                     VKK"
     gene            4150..4965
                     /locus_tag="P5516_07440"
     CDS             4150..4965
                     /locus_tag="P5516_07440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92304.1"
                     /translation="MAEQNQETSKDLLLSDVFIRAARYWWLVTILMIAGGVAGLLIAL
                     LQKPLYESTSQVTTVIDFAYANRLKDYEEDFLLSAIGDIITSDEVIQATATAAVTLGL
                     FTNPDEVTRGMTASRQGYRWQLSTRFNDAHASQKVNQLWLDSSMRALENLRQQSIKAL
                     AQINTEAQVQACFQQSVVLEPVSSYCTVDEIRALLTQPESSNNATSSLSLLNRLLISR
                     ISFQATREAELPDQPVHIGLNTATLAGAAIGLLIAIILFVLGYPRVLRTGCQK"
     gene            4962..6410
                     /locus_tag="P5516_07445"
     CDS             4962..6410
                     /locus_tag="P5516_07445"
                     /inference="COORDINATES: protein motif:HMM:NF016800.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HRT92305.1"
                     /translation="MSSRFWLKSNWYCWALLVITLPITTFPVVMKLTGSASVAPAALL
                     PLIPLVLFFLPAYFWQKKALPVQAKTVLLFFLFALFTIALAFLRPLPLYKAQSPLAAG
                     MKGVVTLLLGFLFYLVAVSVPSSPEKIDKTLRLVNWSGLVVVLWSILQIVLEPLFPAS
                     KVALNQIQSFISPTRLLDDRMQGFASEPSWLAHMLNLVFLAYWLAASYNRTSVHLFRI
                     WRFSFENLLALLGIVALAGTLSRGGILAFLLVLAVIFVLLNIRMVKSLIRKWGQPKNG
                     FRIAGITLGLVLVYLSLIAAALWGWSRLDARMEKVFLLSTKGENPLIEYADNLRFGER
                     VIYWQTGWNIFNDHPLIGVGVGFSGFYFPEYLPDAGWGLSETRKLMFRSEGLLNIKNL
                     WSRLLAETGIIGFALFLTFLVLFAFTSLEMVVKGDGRRKTLGFMGFFMLAALVIEEFS
                     VDSFALPYLWFSMGLVTAGWRWYTFKNGGEHG"
     gene            6403..8436
                     /locus_tag="P5516_07450"
     CDS             6403..8436
                     /locus_tag="P5516_07450"
                     /inference="COORDINATES: protein motif:HMM:NF017368.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="immune inhibitor A"
                     /protein_id="HRT92306.1"
                     /translation="MDKSEKLVLLVFLVILVSACCLLVACGGMALLLSSVSGFLSPTA
                     DTVTFADGTPTPGSPSSVIQPSPMATKLSSEDFERIYHTLSTLENTFIPSADLIYLAE
                     RFEGKKGIPRQLTTPPVAYEVGDRLDFYKINTDTNVSSVTTAVLRYASDTLYFWVEEG
                     VKIDQKELQKTLDVFESKIYPTNQEFFGTEWIPGVDNDPHLYILYAGDLGYSLAGYNA
                     SSDGVLPLAYKNSNAHEMFYINSDVQTLSDPYTLSVMAHEFQHLIHGYHDPNEELWIN
                     EGFSELATLLNGYSAGGFDYLFSYDTDVQLNDWSTESGKNDVHYGASFLYVTYLLDRF
                     GEEITKEIVADQLDGFTSIDHVLAKNGLTDPDTGKIINADDFFVDWTIANLMNDTWFD
                     DGRYAYRIYASAPTASITETLTSCAGKQLDRTVKQYGTDYIELDCPGEEISLQFSGSP
                     TVPVLPISSGENHFMWSNRADGSVTRLGREFDLTGVSAPITLRYETWYDLEVDYDYVY
                     LLASIDGENWQIISTPSCSRQNVSGNNYGCGYNGYSGGWIEQEVDLSAYAGKKVTLSF
                     EYVTDEAVTAEGFLLDNIQLDALNYQSDFESDDGGWLPEGFVNIENLIPQSFLVTILD
                     TSGKSKIQKYALDAGDEISVTLPPLPSGQTYVVLVSGSARFTRQEASYRIDLR"
     gene            complement(8757..8972)
                     /locus_tag="P5516_07455"
     CDS             complement(8757..8972)
                     /locus_tag="P5516_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024143.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HRT92307.1"
                     /translation="MKSIGKFLTGFFLGGIVGALAVLLFTPEPGDKMRQRIKDNCLHV
                     RDEVQNAARQRSEELKKELARLQKKTE"
     gene            complement(9024..10001)
                     /locus_tag="P5516_07460"
     CDS             complement(9024..10001)
                     /locus_tag="P5516_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013718.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HRT92308.1"
                     /translation="MEALIDFEILVTLFWQNLGGWLEYPMQAFTFLGSELFFLLVMPA
                     LYWSIDPVIGFRAGMMLILSGGLNSILKMLFLTPRPYWVDSGVRAYSSETSFGMPSGH
                     SQNSAAIWGIIATTIRKKWAYVFAVLLVFFIGLSRIYLGVHFLHDVLTGWLAGVLVVL
                     LYLKLEAPVSSWLKKRALGTQLLISFIFAITLILLGFLSQQSAGRWVVPTTWTTLAEA
                     AGAEAPDPLNLDGVITIAGVAFGFTAGFAWWRKKFGAYEIKCSLVKKMARYAFGLIGI
                     VVLYFGLKLVFPEEPLLIGGIFRFIRYTLIGMWVTFFAPWFFRLFHLER"
     gene            complement(10038..>10719)
                     /locus_tag="P5516_07465"
     CDS             complement(10038..>10719)
                     /locus_tag="P5516_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HRT92309.1"
                     /translation="LTFPQELLDYQYTPEDGSLDRLSHYSRLLLEKIPDEYMEAAAAH
                     ICPLDGDSQIKLSAMLEKGSIRNVTLQPHRSTMVPERIEDVVVLSKDATAIFVKEAEL
                     RNLFRIRAHDLWEMTAALCSYGCRAVVVKGQQGGYLLHDAYSSHKYLVPDYPVQASDP
                     TGENEVFCGAFLMAYQHTYDPLSAAITGSATASLKREASGPFSLSASLPGLAEARMET
                     IRNRVVQV"
BASE COUNT         2600 a         2710 c         2795 g         2614 t
ORIGIN      
        1 tcaccgagaa atgcaatggc agcggcctgg gcagtagggc ggtaatcccg gaattcatcg
       61 cagccgaaag gcaacagcaa cgtaccgtca ttatgcccca ggttgggcag ccggccgctg
      121 agcgggtcaa gctgggcgag catccaacgg ctggcggcag ccagtttacg cattacagcc
      181 ggcggcagct ctctgttttg ctgacgcatc cgcgcgtggt agagcaacga cagctgcagc
      241 attaaacggt gataattggc gctgtgttga gcataggtgc catcctcttc cacctgccgc
      301 agcaaggcgt gattaaaata actttcgccc tggcggctcc attcccgcgc cctgggatgc
      361 agattggcga ggaaatcgcc cgccagcatc aagcaaaggg actcgctcaa gatatgattg
      421 ttgtgctgag atctggcgta gttcagggtg ggcagaatac gctccacatg atgaactatc
      481 gctgtggtga gtctttccaa ccgttgcgag gttgaaaaag acgaagagtg gaaggcggag
      541 attgcgatga tccacatcat cgcccgcatg ccaacctctt gggcggatac ccagttagga
      601 ccggcgttca ccgtgttgtt gaggttgaac tcgtcaaatt tttgccagaa gagctcagcc
      661 gcagattcct gcgctttacc gcggtagacg cccgccaggg tcaggctcca aaaaaaacgc
      721 gccggttccc acaccatctt gatatcgtca aagcgctcct cgttcacgtc gatccagtgg
      781 acactggatt gcgaaggagg ttcaaggtgg agaatttgcg aggtcgcgta gaacgggcgg
      841 taatatccgt ctttgagaag gcgcgcttct tcaatcatca accttgcgga aaacttaccg
      901 gagagacgct cccaacagga gcgccagcaa aattcaggcg ctgcagatac taaaaattgt
      961 tcaggcgcgg gtaaaccgcc gagaggagtg agtttgcgcg tgtacccact gcgcaactgc
     1021 gcctggtaga gcgaataact gaccaggtca gccaccccga gttcttttat cagcaggaaa
     1081 agtttacgca gttgattcaa ctcgattcgt ccgctttaag gcggctcaaa ccttcatttc
     1141 agtggcagtg cgcagcgctt gaagcgctgc gaagcttgcc cgcgaggtgt tgagtaactc
     1201 atcataagga atgggaggag taccaccctt tttaaccgct gccaggaaag cagcccagga
     1261 ttgcgcgtga cctttgtcct ggcgcagaac ggagcgtgat gtctttcgtt cattttctga
     1321 aaccagcgca agcgaacgga aatcttccaa tacgccaatc ttgccgctgg aaaacacctc
     1381 gatcctctcc ttgggaaagt tcttgttgcc gttcgccagg taagccactg tgccgattga
     1441 gccatccgcg taacgaaagg tcagctgcac gttgtcttgc cggtacttcg cttgatctgc
     1501 caatgccgcc gcgctcaccg atacaggtac agcgccaatt agaaaggtga gcagatcgat
     1561 gaaatggcag gcttcgccga tgatacgccc gccgccctgc tctgggtcat gcaaccaatg
     1621 attcaatggc agaaagcccg cgtttacccg gtaatgggca tataaaggct ccgtccgctg
     1681 agcgatgaag tttttaagag tgagggtcaa cggggcaaaa cggcggttat aacccacgct
     1741 gaggagtgga gcgtctgccc tggcgagttc tgcttcgatc tctgaaagtt cttcagcgtt
     1801 taatgccagc ggtttctcgc aatacacagc tttgtgatgg cgtaacgcgc tcaacacctg
     1861 gcgggcgtga tgttgatgcc gtgtgagcaa cactaccgtg ttgatatccg cgttgtccag
     1921 aatctcctct tctgaggttg tggcaaattc gaaaccgaac ttcctggcag cctgttgggc
     1981 gctcataccc gaagcagtgg caatgcccac cagttgcgct ccgggggttt ttttgatcac
     2041 cggcagaaaa accgcctggg cgtaatttcc cgcgcccaac acgccgagct tgatggcggc
     2101 aggcacgttg gcggctgacg gcgtaaccag cactttctgc tggttgctga agtcttcttt
     2161 taccgggtat gtgaggagaa cgcccatgaa aggctctgat cgtttgccag agatgaggtc
     2221 ataagcgctg acagcgtctt cgataggtat gcggtgagaa ataagagggc gcacgtccac
     2281 cttgccagtg tgcatcaggt caatgaacgc ctgtaaattt cgtccttctg tccagcgcac
     2341 atagcctggc ggataatcga ttcctccttc ttcgtagaca gggtcatacc tgccaggtcc
     2401 gtaagaacgg gaaatcttaa aaaccagttc tttttcatag tagcgttttc gcgggatttg
     2461 catccccacg gcgccaacag ccaccactgt accgcggtca cgagcgatat cccctgccag
     2521 ttcaactggg tcctgcgaag gcgtatccgc gcagatcaac acgtggtcaa aaccgagccc
     2581 gttggtcacg ccggcggcca ggttcgcgca ttgttgtcgt tgggcggcgg agaaacccag
     2641 cgcttgcgcc aggctgaccc gctcctcgct gagatcgata ccaaacacga ggcagcctgc
     2701 ggctcgcgca atttgggcgg agagcaaacc taacagacct aaaccgatga cacaaacact
     2761 ttctcccaat tggggagaag ccaggtggaa gccatgcagc gcgatcgcgc caagagtggt
     2821 gaaggcggca gattcgaaat ctacattttg cggcaggcgc gctgccaggt tcaccgggac
     2881 gagcgcatac tccgcgtgca cggcataacc accacctgcg caggccaccc gatcgccggc
     2941 tttaaaatcc agcgcatctt cacccgcctt gacaactatc cctgacgagg agtatcccaa
     3001 cggcattggc tgatcaagtt tattgaaagc cgcttctact gtgggaaaga gtccttcgcg
     3061 catggctttt tgaaccacct gcttaaccag gtctggtcga gaggtagctt taccaaccag
     3121 gttttttgag gcaaaatcga cgaccatacg ctctgttccc gcagaaacga gagaacaggc
     3181 ggtttgcacc agcaccatgc cgcgcctgac ggcgggaaca ggcacttctt cgacaaccgt
     3241 ttttccattg tttaagtact gaagtatctg tttcatggtt tctcctatat cagattatat
     3301 tcgattatct gcagcgttga ccttctccct cattcgcctt tttgaaacat gcagaatggc
     3361 atggaaagtg cattaaaaac ccttacagct cgtgcaaggt cggaggaagg tatgttgaaa
     3421 aagatactgt ggatattaat gctgcttatc atgaccgcct gcaccatcct gtttaaagat
     3481 ggtgtgaata ttgtattggg tgagctcaca cctaccatgg tcgctacatc aaccccggtg
     3541 gatattggcg tggaaattac acccaccgag acggttgaac caacaatgga accaacggct
     3601 actgaagaac cagttaatct gccttcaccg acaatagtaa caacggctga accagaaccc
     3661 acccccacgc ctgaagcaac ggctacccct tttgtcgcgg tttacgaagt gcaaaagggc
     3721 tctccagcct acctggataa tttcgctcat ctctccgatg ggtgcgcttg gcaggggatc
     3781 gcgggtcagg tgttcaacag taaaaaggag ccggttacaa acctggttgt gaaggtcagc
     3841 ggtgaatgga atggaaagaa gattgccagt gttggtgtta caggaatggt cgccggcctg
     3901 ccttacggtc cgggcagtta tgagattgtg ctgggagaca aggcggtcaa ttcaaacgcc
     3961 accctgtacg tgcagctttt tgatgaaggg caaaaaccat taaccgaacc gaatgctgtt
     4021 tcaactcagg cggattgcca aaaaaacctg gtgatcttaa attttgtgaa gaaataatac
     4081 gaaagcgcgc attgagcgca ggccccaggt gatgccacgc gtgttctgtg gcagtgtata
     4141 attcagccaa tggcagagca aaatcaagag accagcaaag acctgctgct gagtgatgtt
     4201 ttcataaggg cagcgcgtta ttggtggctt gtgaccatcc tgatgatagc cgggggggta
     4261 gcaggtctgc tgattgcgct tttacagaaa ccgctctatg aatccacttc ccaggtcacc
     4321 acggtgatcg attttgcata tgccaaccgc ctgaaagatt acgaagagga ttttcttctc
     4381 tctgccattg gagatattat cacctcagac gaggtgatcc aagcaactgc caccgctgct
     4441 gttacacttg gcctgttcac gaacccggac gaagtaacca ggggaatgac ggcttcaagg
     4501 cagggttacc gttggcaatt aagcactcgc ttcaacgacg cgcatgcctc ccaaaaagtg
     4561 aaccagctat ggctggattc ctcgatgcgg gcgcttgaaa atctccgcca gcagtccatc
     4621 aaagcgctgg cgcagataaa cacagaagcg caggtgcaag cctgttttca acagtctgta
     4681 gtcctcgagc ccgtttcttc ctattgcact gttgatgaaa tacgcgcgct gctgacccag
     4741 ccagaaagtt caaacaatgc cacctcgtcg ctttcattac tgaacaggct gttaatctct
     4801 cgtatcagct ttcaggcaac gcgcgaggct gagctgcctg accaacccgt tcacattggg
     4861 ttgaacaccg ccacactcgc tggcgcggct attgggctgc ttatcgctat catcctgttc
     4921 gttttgggtt acccgcgggt tcttcgcaca ggctgccaga aatgagttcc cggttctggt
     4981 taaaaagcaa ctggtattgt tgggcgctgc tggtgatcac cctgcccatc acaacttttc
     5041 cagtggtgat gaaactgact ggctccgctt cagtcgcacc ggcggcgtta ctacctttaa
     5101 ttcccctggt gctgttcttt ttgcctgctt atttttggca gaaaaaggct ttgcctgtcc
     5161 aggcaaaaac ggtgctgttg ttcttcctgt tcgcgctgtt cactatcgcg ctggcttttc
     5221 tacgaccatt acctctctat aaggcgcaat cgccgcttgc agcggggatg aagggcgtag
     5281 tgaccttgtt gttgggattt cttttttacc tggttgcagt cagcgttccc agttcgcctg
     5341 aaaaaattga caagaccctg cgcctggtca attggagcgg gctggtggtg gtgttgtggt
     5401 ccattcttca gattgttctg gaacccttgt tccccgcttc caaagttgcc cttaatcaga
     5461 ttcagtcttt catttcacct accaggctgc tggatgaccg catgcagggt tttgcttctg
     5521 agccttcttg gctggcccat atgctcaacc tggtttttct ggcctactgg cttgctgcca
     5581 gttacaatcg caccagcgtt cacctgttca ggatctggag gttttctttt gaaaacctgc
     5641 tggcgctgct gggaattgtg gcgctcgcgg ggacgctctc gcgtggcgga attttagctt
     5701 ttctcctggt gctggcagtg atctttgtat tgttgaatat caggatggtc aagtcgctga
     5761 tacgaaagtg gggtcaaccc aaaaacggtt tcagaatcgc tggcatcaca ctgggcctgg
     5821 ttctggtcta cctgtcatta atcgctgctg ccttatgggg atggagccgg ttggacgcca
     5881 ggatggagaa agtttttctc ttatcgacca aaggcgaaaa cccactcatc gaatacgcgg
     5941 acaatctacg tttcggggag cgggtgattt actggcaaac cggctggaat atttttaacg
     6001 accatcccct catcggtgtt ggggttggtt tttcggggtt ttacttcccg gaatatctac
     6061 cggatgcggg gtgggggttg agtgaaactc gcaaattaat gttccggtct gaaggcttgt
     6121 tgaacataaa aaacctgtgg tcgcggctgc tggctgagac gggaatcatt ggtttcgcat
     6181 tgttcctcac ttttctggtg ctctttgcct tcacctctct ggaaatggtc gtaaagggtg
     6241 atgggagacg aaaaacattg ggttttatgg ggtttttcat gctggctgcc ttggtaattg
     6301 aagaatttag cgtggacagt ttcgcgctgc cctatttatg gttcagtatg gggttggtca
     6361 ccgcgggttg gcgttggtac acttttaaga atggtggaga acatggataa atctgaaaag
     6421 ttggttttac tggtttttct ggtcatatta gtctcggctt gctgcctgct ggtggcttgc
     6481 ggcgggatgg ctcttctgtt atcaagcgta tcgggattcc tttctccgac agcagacacg
     6541 gttacattcg cggatggtac gccaacgccg ggtagcccca gctcagtaat acaaccttcg
     6601 ccaatggcga caaaattatc ttctgaggat tttgagagga tctaccacac gctctccacg
     6661 ctggaaaaca ccttcatccc aagcgctgat ttaatttacc tggctgaacg ctttgaaggc
     6721 aaaaaaggca ttccccggca gttgaccaca cccccggtgg cttacgaagt gggagaccgg
     6781 ctggattttt acaagatcaa cacagatacc aacgtctcca gtgttaccac tgccgtgttg
     6841 cgctatgctt cagacacatt atatttctgg gttgaagagg gtgtaaaaat tgaccagaaa
     6901 gaattgcaga aaacgctcga cgtctttgaa agcaagatct acccaaccaa ccaggaattt
     6961 tttggaacag aatggatccc gggggttgat aacgacccgc acctttacat tttatacgcc
     7021 ggagatttag gttattccct ggcaggctac aacgcatctt cggatggcgt actgccactg
     7081 gcgtacaaga attcgaatgc gcatgaaatg ttttatatca attcggatgt tcaaaccctt
     7141 tcagacccct acaccctgag cgtgatggcg catgaattcc aacatctgat tcatggctat
     7201 catgacccta acgaggagtt gtggataaat gaaggcttct cggaactggc aaccctgttg
     7261 aatgggtaca gcgcaggtgg gtttgattat ctcttttcct acgacacaga tgtgcaattg
     7321 aacgactggt caacagaatc tggaaaaaac gatgtgcatt acggcgccag ttttctttat
     7381 gtcacctacc tgctggaccg atttggagaa gagattacca aggaaattgt cgctgaccag
     7441 cttgatggat ttaccagtat tgatcatgtt ctggcgaaaa atggcttgac cgacccagac
     7501 acagggaaga ttatcaacgc tgatgacttc tttgtggatt ggacaatcgc gaacctgatg
     7561 aatgatacct ggttcgacga tggtcggtac gcctatcgta tttacgcatc cgcccccaca
     7621 gccagcatca cagaaacgct tacctcctgt gctggtaaac agctggatcg gacggtgaaa
     7681 caatatggaa cagattatat tgaacttgat tgccctggcg aagagatctc gctgcagttc
     7741 tctggttcac caacagtccc tgtgctgcca atctccagcg gagaaaacca cttcatgtgg
     7801 tccaaccgcg cggatggctc ggttaccagg ctgggccggg agtttgatct aaccggggtc
     7861 agcgcaccta taaccctgcg ctatgaaacc tggtacgacc tggaggttga ttatgactat
     7921 gtgtacctgt tggcatcaat tgatggagag aactggcaaa taatttctac accgtcctgc
     7981 agccgacaga acgtttccgg taataattac ggttgtggtt acaacggcta ctcaggcggc
     8041 tggatcgagc aggaagtgga cctttctgct tacgcgggga agaaggttac tttaagtttt
     8101 gagtacgtga cagatgaagc ggttactgcg gaaggttttt tgctcgacaa tattcaattg
     8161 gatgcactca actaccagtc tgattttgag tcagacgatg gcggctggct gccagagggg
     8221 tttgtgaata tcgaaaacct gatcccgcag agctttttag taaccatact ggatacgtct
     8281 ggtaagtcaa agatacagaa gtacgccctt gatgccgggg atgaaatttc agtcaccctg
     8341 cctcctttgc cttctggtca aacgtacgtg gtacttgtca gcgggtccgc cagattcacc
     8401 cggcaagagg catcctatag aattgacttg cggtgaagtg ttgcgttttc ggatattacc
     8461 tggtcggaaa acaggtcggc tttttttaag ccgctgccat cttcctgaat gaggttatgg
     8521 acgtcttgcc aggcttgagg gcgtgctttc tcaccgcccc agcgagatgg cggataaacc
     8581 taggagcaac ctctgatggc ttgatggtca tattgcgtga tagaaaaaag ggcaaacttg
     8641 ctttaaaaca cccggcactg cataacgttt tttcccgcca gaaaaaacct gagaaataag
     8701 agggcgtgaa aagaaaagca gttgctggag ttcaagcaac tgcttttggt tgaagatcac
     8761 tcggtttttt tctgtaagcg cgccagttcc ttttttaatt cttcactgcg ttggcgggcg
     8821 gcgttttgca cctcgtcacg cacatgtaaa cagttgtctt taattcgctg gcgcattttg
     8881 tccccgggtt caggggtgaa taaaagcacc gccaacgcac ccacgatacc gcccaggaaa
     8941 aaaccagtca agaactttcc gatggatttc atagttcctc caatttctca attcacttaa
     9001 ttatagccct ttatggcgtg taattaacgt tcgaggtgaa acagtcgaaa aaaccatggc
     9061 gcgaagaagg taacccacat cccgatgagc gtatacctga tgaaacgaaa tattccgcca
     9121 atcagcaagg gttcttcagg aaagacgagt ttcaaaccaa aatagaggac aacaatgcct
     9181 atcaaaccaa atgcataacg cgccattttt ttaacaagcg agcatttgat ctcatatgcg
     9241 ccgaatttct tacgccacca agcaaaccca gcggtaaaac caaaagccac cccggcgatg
     9301 gtgatcaccc catcaaggtt gagtgggtcg ggggcttcag cgccggctgc ctctgccaaa
     9361 gtggtccagg tagtgggtac cacccacctg ccagctgatt gctgtgacaa aaatcccagc
     9421 aaaattaagg taattgcaaa tatgaatgaa atcagtagct gggtgccgag tgctcgtttt
     9481 ttaagccagc tggatactgg cgcttcgagc ttgagataga gcagcacgac cagcaccccg
     9541 gcaagccaac ccgttaatac gtcatgcaga aagtgaacac ccaggtaaat gcgtgaaaga
     9601 ccaataaaga agaccagcag caccgcgaag acgtaagccc attttttacg gatagtcgtg
     9661 gcgatgatac cccagatggc tgccgaattt tgagaatgcc cagaaggcat cccaaatgaa
     9721 gtttctgagg aataggcgcg tacaccactg tcaacccagt atgggcgtgg agtaagaaag
     9781 agcatcttga ggatggagtt caaacctccg ctaagaatta acatcatccc ggcgcgaaaa
     9841 ccaataaccg ggtcaatgct ccagtaaagc gccggcatca ccagtaaaaa gaacagttcg
     9901 ctgcccagaa aggtgaacgc ctgcatgggg tattccaacc aaccgcccag gttttgccaa
     9961 aacagtgtga caagaatttc aaaatcaatt aacgcttcca ttgtttcctc ccaaatgatg
    10021 agccaatgaa gtcaagttta aacctgtaca acccgattgc ggatggtttc catacgggct
    10081 tcagccaacc caggcaacga agcagataga gaaaacgggc cgctggcttc tcttttgagt
    10141 gaagcggtgg cgctgccggt aattgccgcg ctgagggggt cataggtgtg ctggtaagcc
    10201 atcaggaacg cgccgcagaa aacctcgttt tcgcctgttg ggtcgctcgc ctgcactgga
    10261 taatctggaa ccaaatactt gtgtgaagaa taagcatcat ggagcaggta tccgccctgt
    10321 tgtcctttaa ccactaccgc ccgacagcca taactgcaca gagctgctgt catctcccac
    10381 aggtcatgag cccggatgcg aaagaggttg cgcagttccg cttctttcac gaaaattgcg
    10441 gttgcgtctt tgcttaatac aacgacatct tcaattcgtt ccggcaccat ggttgaccga
    10501 tgaggttgca aggtgacatt ccgaatcgag cctttctcca acatggcaga cagtttaatc
    10561 tgactgtcgc catcaagcgg gcatatatgc gccgcggcgg cttccatata ctcatccggt
    10621 atcttttcca gcagaagacg ggagtaatgg gagagcctat cgagcgaccc atcttcagga
    10681 gtgtattggt aatccaataa ttcctgagga aaggtcagg
//
LOCUS       DAOTLW010000050        10277 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_6828_length_10277_cov_10.096067, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000050 DAOTLW010000000
VERSION     DAOTLW010000050.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10277)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10277
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6828_length_10277_cov_10.096067"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..200
                     /locus_tag="P5516_07470"
     CDS             <1..200
                     /locus_tag="P5516_07470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92310.1"
                     /translation="NDGKLVMSLNGQSITMNYTLEDPDVIILVGSLDGTIPDQKMTYR
                     VEENKLTFTLDGADTVFYRVK"
     gene            336..1547
                     /locus_tag="P5516_07475"
     CDS             336..1547
                     /locus_tag="P5516_07475"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HRT92311.1"
                     /translation="MMQKKILFKILVVVAVFTLGLTSCNLMGYIQKNDADDFTQIETM
                     AAATISARLTQSAFDELAMQLTQQAPTLSLPTSQLPLSSSTPIPTSTPIASFTPIATS
                     IPPTATPIPIPCNAAKYLADVTIPDWSTVYAGEAFVKTWQVKNVGTCSWTKDYHIYFA
                     DGKKLDAPTSVAFPKVVNPGETVELSVPMVAPSDKGSFTGSWMLEAANGVRFGVGYTY
                     NVPLTVNIKVENMPAPKDPHTRYDLVKEMCSASWRTNAGDIGCPSAKVDTDKGSITRS
                     YAPVIAGGYKDDEGALYTVPAKGGDGMILGRFPKFKLLSGDRFRALLICADKAQKCSV
                     TYELLYKVSGSDESGSLGTWDVNYSNSTQVDVNLDSLADKEVHLMLKVYSKGDSTDDV
                     ALWMAARVTNP"
     gene            complement(1624..3294)
                     /locus_tag="P5516_07480"
     CDS             complement(1624..3294)
                     /locus_tag="P5516_07480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HRT92312.1"
                     /translation="MKRIERVEHWRKARQYTSEQLEQAKIVLEEVRAGVRLDRALRAN
                     PMPDGALIAKHILVAAYRELVAEGEWPEDPSFLSRIRMKPVRTLSGVTTVTVLTKASP
                     CPGECIFCPSEQQMPKSYLSDEPGAARAYQNRFDPFDQVSSRIHEYEAVGHPNDKIEL
                     LVLGGSWSCYPRAYQEWFIRRCFHAMNADSTLPGDEPVDTSEPPAGARPQDDLVELRK
                     AHTRNENALHRNVGLVIETRPDDIDRDELVWLRRLGVTKVQMGAQSLDDRILQLNRRG
                     HSSADTLRAVTLLRAAGFKIVLHWMPNLLGATPASDREDFARLWKDGYAPDELKIYPT
                     QLLESAELYHYWQRGEYTPYTTEQLIDLLADLKPLIPPYCRVNRVIRDIPSFHVVEGN
                     RRTSLRQDVQRELQRRGTACRCIRCREVKGESVSSAELAFSDYVYQPLTSEEHFLSYT
                     TPEDRLAGYLRLSFALPGAPDLAIPELDSAAIIREVHVYGQSLPVGTERDGAAQHSGL
                     GTSLLDHASRITRARGLSRLAVIAAVGTRAYYEKRGFHQQELYMVKEL"
     gene            3314..4075
                     /locus_tag="P5516_07485"
     CDS             3314..4075
                     /locus_tag="P5516_07485"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRT92313.1"
                     /translation="MRQDIFQRLLNINREFYQSFGQAFAQTRRRVQPGVRRALAQWIT
                     NGDWLDIGCGSGTLSREWRSAGLKGSYTGVDFSQPLLKEAAALQQNPLESDELTVSFL
                     QADLTTPDWTSVLPRRMYDGVVAFASLHHIPSAAYRKQLLDTIARMIKPGGLLVLSVW
                     QFQHSPRLMARVLPWRLADMAEEDLEEGDTLLDWRYHPGESGAAPGLRYVHLFTPEEL
                     QALARKTGFEVVTAYESDGSGGRLGLYQVWQRAAL"
     gene            complement(4070..4483)
                     /locus_tag="P5516_07490"
     CDS             complement(4070..4483)
                     /locus_tag="P5516_07490"
                     /inference="COORDINATES: protein motif:HMM:NF012422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HRT92314.1"
                     /translation="MTRLLYLPDDATVIQLEVDLSPAELVAAVNAGLRPLPILRTAPA
                     GKLSASQVNATVVIAPLRSPGGPRRNSVNANLTRRQRQVLELQSRGFNSAEIAAMLNL
                     SRRAVNYHLNQVRQRIRGELPELLKSDFDELESLL"
     gene            complement(4723..6171)
                     /locus_tag="P5516_07495"
     CDS             complement(4723..6171)
                     /locus_tag="P5516_07495"
                     /inference="COORDINATES: protein motif:HMM:NF015563.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HRT92315.1"
                     /translation="MESKAGAQISQKAFIQSAVILLVLMIAAGILTRVIPAGSYQRYQ
                     VEGRSLIDPASYQVIERPDYPIWRWFSAPVEVLWGEDSLIIITIIVFIVLVSGAFAVL
                     EKSGILTALLGKIVKRFRSRRYVLLLVIALFFMLLGGFFGLLEEIIPLVPLLIALSYS
                     LGWDALVGLGMSILATNIGFSAAITNPFTIGVAQRLAGLPLFSGALLRLPVFLVFYAA
                     LAWFLLSYARRIDRDPASSLLYKEDQEKGTRKPLFDLADTDSRSGIGKAALVFGFFVL
                     LILAVLLTGPFVPTIADYTLPLVGVLFFAGGLVSGFVSGCPGRLIGRALKEGILGLAP
                     GIPLILMAASTKHIVSNGKVIDTLLYQGSHLLEGASPLSAVLIIFLLALVVEFFVASA
                     GAKAVLMMPILLPLADLVGVTRQLTVSAYCFGDGFTNLAYPTNAILLICIGLASISYG
                     KWIRFTAKLWLMVFPLVVFFLWIGSLIHFGPF"
     gene            6293..7024
                     /locus_tag="P5516_07500"
     CDS             6293..7024
                     /locus_tag="P5516_07500"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013980.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family zinc-dependent metalloprotease"
                     /protein_id="HRT92316.1"
                     /translation="MTEPIESVTTIAGRRIPYRLVRSLRASRMRMTIKESQVTVTLPR
                     GVPPSEAERFLQQNAAWLFKQLDKPASRKKPLPALPKDVILLRGEATHIQVIEEPTRS
                     TRMRVDEAGGRLVVRVPAGTRESPRRLVVPWLKAQARGTIEVAVKQLAVRMGVTYKSV
                     SIRDQRTRWGSCSSQGALSFNWRLVMVPPAVLEYVVTHELAHRKQQNHSRAFWQVVAL
                     YFPHFKAARTWLRANAALLRPKDTI"
     gene            7051..8070
                     /locus_tag="P5516_07505"
     CDS             7051..8070
                     /locus_tag="P5516_07505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT92317.1"
                     /translation="MEFTHHTEFPYEMATEWNALLAESATHVPFLRCEYLADWWATRG
                     GGEWPDDSLLAIITAREDGRLIGIAPFFITSHEDKKKLLLLGSIEISDYLDVICRAEE
                     INRFLHGLLRFIREQFVATGQITGLDLYNIIEDSPTLKALEESAKTLDLEMRVRKLQH
                     SPFIPLPGNWETYLERLEKKQRHEIRRKMRRALEGDAQVKLYFTQDEGNLEDDMEDFL
                     ELMAQDEAKEDFLSPMMREQMKSVMRCAFAQDCLQLAFLEIGGQKAAAYLSFDFLNRI
                     WVYNSGIDRRYMEYSPGWVLLGYLLKWANENGRETFDFMRGNEDYKYKFGALDRFVMR
                     VSLEF"
     gene            complement(8135..9613)
                     /locus_tag="P5516_07510"
     CDS             complement(8135..9613)
                     /locus_tag="P5516_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013696.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family peptidase"
                     /protein_id="HRT92318.1"
                     /translation="MQFFLPLVFLILVLAAVIAARTFLLIRSAGHKPLPFQSKLPDPP
                     LDRQKIAEHLSAAIQIETVSHEDPAKNRTENFERLHDLLQRTYPRLHGSLSREIVQGF
                     SLLYTWQGSDPALEPVVFMAHQDVVPIDEHTLEQWTHPPFSGRIADGFIWGRGSLDIK
                     NQLIAVMEAVELLLEQGYQPVRTVLLSFSHDEEVLGHGAPAIVRHLKEKGISVHAVLD
                     EGNAVLDGVLPGFKGHTAPIGVAEKGYLSLKLTVEASGGHSSTPAAETAIGVLARAID
                     RLQSHPFPYRLNTVYPMFKGLSPAAPRLLQVAFANLWLFGGLVRRQLAAQAETAAAIH
                     TTTAPTLFHGGVKDNVLPTHAEAVVNFRLLPGDSVAEVTEQVQSVINDSRVRIEPLPE
                     YAWEPSPVSPTDTPAYQHIVSVLNELFTDIACAPSNMLGATDARNYHAISSRVYRFSP
                     LLLHKKDLERIHGINERISLENMHKMVSFFHRLIQRWSSGEM"
     gene            complement(9761..10201)
                     /locus_tag="P5516_07515"
     CDS             complement(9761..10201)
                     /locus_tag="P5516_07515"
                     /inference="COORDINATES: protein motif:HMM:NF012780.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HRT92319.1"
                     /translation="MATVASILKKKGSQVYSVSPETSIIDCLKLMSEKKIGSVLVMQD
                     EKVVGIFSERDYAWRGILKGNHEHTPVQRVMTEKVYYVSPEQSTDTCMAQMSDKHIRH
                     LPVLDKGKVVGVISIGDVVTSMLQDKETLIKGLENFILSNEIKL"
BASE COUNT         2381 a         2725 c         3006 g         2165 t
ORIGIN      
        1 gcaatgacgg caaactggtg atgagcctga acggtcagag catcaccatg aactataccc
       61 tcgaagaccc cgatgtcatc atcctggtgg gcagcctgga tggcaccatc cccgaccaga
      121 aaatgaccta tcgcgtagaa gagaacaaac tcaccttcac actggacggc gctgacacag
      181 tcttctaccg tgtgaaataa aaaacaccgc tccggcctgt cgcgccgggc gttccgattc
      241 tcactttaag tagtaatatg aaggagaaac tcttgatcaa aggcgggaac atttcgctcg
      301 cggttgcgtc ttaagcaaga tataaacgga ggtttatgat gcaaaagaaa attctcttta
      361 aaatattggt cgttgtggcg gttttcacgc ttggattgac atcctgtaac ctgatggggt
      421 acattcagaa aaacgacgcg gatgatttta cccaaatcga gacaatggcg gcggcgacca
      481 tctcggcgcg cttgacacag tcggcttttg acgagctggc catgcaattg acgcaacagg
      541 cgcccacact gtctttgccc acatcacagc tgccgctgtc ctcaagcacg ccgatcccca
      601 ccagcacgcc tattgccagt ttcactccta tcgccacgag cattccaccc acagcgacgc
      661 cgatccccat cccctgcaat gctgccaagt acctcgctga tgtcaccatt ccagattgga
      721 gcacggtcta cgcgggtgaa gcgttcgtga agacctggca ggtaaaaaat gtgggaacct
      781 gttcctggac aaaagattac catatctact tcgctgacgg caagaaactg gacgcgccta
      841 cttcagtcgc cttccccaag gtggtaaacc cgggtgaaac cgttgagctt tccgtgccga
      901 tggtggctcc ttctgataaa ggcagcttca ccggcagctg gatgcttgaa gcagctaatg
      961 gcgtgcgttt tggcgttggt tacacctaca acgttccgct cacggtgaac atcaaggttg
     1021 agaatatgcc cgcgcccaag gatccccaca cccgctatga cctggtcaag gagatgtgct
     1081 ccgcctcgtg gcgcacgaac gctggcgata ttggctgccc gagcgccaag gttgatacag
     1141 acaaaggctc aattactcgc tcttacgcgc cggtgatcgc tggaggttac aaggatgacg
     1201 aaggcgcatt gtacaccgtg cccgcaaaag gcggcgatgg gatgatcctg ggcaggttcc
     1261 ccaagtttaa attactgtca ggagaccgtt tcagggcgct gctgatatgc gctgacaagg
     1321 cgcagaagtg ttccgtcacg tatgaattgc tttataaggt gtcggggagc gatgagagcg
     1381 gatcgctggg tacgtgggat gttaactact caaactccac ccaggtggac gtgaacctgg
     1441 attcgctggc cgataaagaa gttcacttaa tgttgaaagt gtacagtaaa ggagactcga
     1501 cggatgacgt cgcgttgtgg atggccgcca gggtgaccaa tccataagat gtaaaagacc
     1561 caacacgagg ctgcctcacc tattgaagca gcctcgtgtt ttttgaaata agtcgttcag
     1621 acgctacaac tctttcacca tatagagctc ttgctgatga aaaccacgtt tttcataata
     1681 ggcgcgcgta cccaccgcgg cgatcaccgc caggcgcgaa agaccccgcg cgcgtgtgat
     1741 acggctcgcg tgatcgagca ggctggtacc cagcccgctg tgctgcgccg ccccgtcgcg
     1801 ctccgtgccc accggtaacg attgaccgta gacgtgcacc tcgcgtatga tggcggcgga
     1861 atcgagttcg ggaatggcga gatcaggcgc acccggcagc gcgaatgaca gccgcaggta
     1921 gcctgccagc cgatcttcag gggtggtgta agaaagaaaa tgttcctcgg aagtcaaagg
     1981 ctggtaaaca taatcagaaa aggcgagttc agcggaagac acagactctc ccttcacttc
     2041 gcggcagcga atgcagcggc aggcagtgcc gcggcgctgc agttcacgct gcacatcctg
     2101 ccgcagactg gtgcgccggt tgccttctac cacgtgaaaa gatgggatgt cccggatgac
     2161 gcggttgacg cggcaatagg gagggatgag aggtttgagg tccgccagca aatcaatcag
     2221 ctgttcggtg gtgtaaggcg tgtattctcc acgctgccag tagtggtaaa gctcagcgga
     2281 ttcaagtaat tgggtggggt aaatcttaag ctcatcgggg gcgtacccgt ctttccaaag
     2341 acgggcaaaa tcctcgcggt cagatgcggg tgtggcgccc agcaggttgg gcatccagtg
     2401 cagcacaatc ttgaatccgg cggcgcgcag cagcgtgacg gcgcgcagcg tatctgcgct
     2461 gctatgaccg cgccggttga gttgcaggat gcggtcatcc aaactttgag cgcccatctg
     2521 cactttggtc acacccagac gacgcagcca gacgagctca tcccggtcga tatcatccgg
     2581 ccgggtttca atgaccagcc ccacgttgcg gtggagcgcg ttttcattgc gcgtatgggc
     2641 ttttcttaat tcaaccaggt catcctgggg gcgggcgccg gcaggtggct cggaagtgtc
     2701 gacaggctca tcaccaggca gggtgctgtc cgcgttcatc gcgtgaaagc agcggcgaat
     2761 gaaccactcc tggtaggcgc gcggatagca gctccagctg ccgcccagca ccagtaattc
     2821 gatcttatca ttggggtgcc cgacggcttc gtactcgtgg atacgcgagc ttacctggtc
     2881 aaaggggtca aaccggtttt gataggcgcg ggcggcgccg ggttcgtccg acagatagct
     2941 cttgggcatt tgctgctcgc tggggcagaa gatgcactct ccagggcagg gagacgcctt
     3001 ggtaaggacg gtgaccgtgg tgacgcctga aagcgtccgt acaggtttca tgcggatgcg
     3061 ggataaaaac gaggggtctt caggccattc accttccgcc accagctcgc ggtaagccgc
     3121 caccaggatg tgtttggcga taagggctcc atcgggcatg gggttggcgc gcagcgcgcg
     3181 atccaggcgg acgccagcgc gcacttcttc gaggacgatt ttcgcctgtt ccagctgttc
     3241 ggaggtatat tggcgcgcct tgcgccagtg ctctacgcgt tcaattcgtt tcatcggaaa
     3301 gtataatggg gctatgcggc aagatatctt tcagcgctta ctgaacatca atagagaatt
     3361 ctaccagagt ttcgggcagg ctttcgccca gacacgacgc cgcgtacaac cgggtgtgcg
     3421 ccgcgcgctg gcgcaatgga tcacaaatgg cgactggctg gatattggct gcggcagtgg
     3481 tacgctttca cgggagtggc gcagcgcggg cttgaaaggc agctacacgg gcgtggattt
     3541 cagccagccg ctgctaaaag aagccgctgc gctgcaacaa aacccgctgg agagcgatga
     3601 actcaccgtc tcctttctgc aggctgacct gaccacgcca gattggacat ccgttttacc
     3661 gcgccggatg tatgatggcg tggttgcctt tgcctcgctg caccatatcc catccgctgc
     3721 ttatcgaaaa cagctgctgg atacaattgc gcgcatgatt aaacccggcg ggctgctggt
     3781 cctttcagtt tggcagtttc agcacagccc caggttgatg gcgcgtgtgc tgccctggcg
     3841 cctggcagac atggctgaag aggacctgga agaaggggac accttgctgg actggcgtta
     3901 tcacccgggg gagtcaggcg cggcgccggg gctgcgctat gtgcacctgt tcacgcctga
     3961 ggagcttcaa gcgctcgcac gcaaaacggg gtttgaggtc gtcacggcgt atgagtcaga
     4021 tggcagcggg ggcaggctgg gattatacca ggtgtggcaa agggcggctt tataaaaggc
     4081 tttcaagttc atcaaagtcg gatttaagca gctcggggag ttcgccgcgg atgcgctgac
     4141 gcacctggtt gaggtgatag ttgaccgcgc gcctggagag gttgagcatg gcggcaattt
     4201 cagcgctgtt gaaaccacgc gattgcagct ccagcacctg gcgctggcgg cgggtgaggt
     4261 tggcgtttac actgttgcgg cgcggacccc cgggtgagcg cagcggagca atcaccaccg
     4321 tggcgttcac ctggctggcg ctcaatttgc cagccggggc ggtgcgcaaa atgggcagcg
     4381 gtcttaaccc ggcgtttacc gcggccacca gttccgccgg gctgagatca acttcaagtt
     4441 ggatgacggt cgcgtcatcc ggcaggtata gcaggcgggt caatgtggtt tccggcagga
     4501 gtgaggacgt gtaacgtgca tgatgaatca cttagataga attagaacta acattctata
     4561 tattaacctg taagaagcac tttgtcaaga caggcgtgga taaactgaaa actcatccgg
     4621 ctggatggtt gaagacccct ggaggcagtg aatttacccg gcagcgggtg cgcgttcaca
     4681 ccatctaaaa aaaaagcaag tggagatggt aaaaacagcc gcctaaaagg gtccaaagtg
     4741 aatcaggctg ccaatccaca agaaaaacac gaccaggggg aagaccatca gccagagttt
     4801 cgcggtgaag cgtatccact taccatagct gatggaagcc aacccgatgc agatgagcag
     4861 gatggcattg gtgggatacg ccaggttggt gaaaccgtcg ccaaaacagt aggcgctgac
     4921 cgtgagctga cgcgtcacgc ccaccaggtc agccagtggt aagaggatcg gcatcatcaa
     4981 aacggctttc gcgccggcgg atgccacaaa gaactccacc accagcgcca gcagaaagat
     5041 aatgagcacc gcggacagcg gcgaagcgcc ttccagcagg tgcgacccct ggtacagcag
     5101 ggtgtcgatg acttttccgt ttgaaacgat gtgtttggtg ctggctgcca tcaggatgag
     5161 cgggatgccg ggagccagac ccaggatccc ttctttaagc gcgcgaccga tgagcctgcc
     5221 tgggcagccg gagacaaatc cgctcaccag cccgccggcg aagaacaaca cgcccaccag
     5281 cggcagggtg taatcagcga tggtggggac aaacggacct gtcagcagca ctgccaggat
     5341 gagcaatacg aaaaatccga ataccagcgc cgctttgcca ataccagatc ggctatccgt
     5401 atctgccagg tcaaaaagtg gcttgcgcgt gcccttctcc tggtcttctt tatagagcag
     5461 tgaagaggcc gggtcgcggt caatacgccg cgcgtatgaa agcaaaaacc aggccagcgc
     5521 ggcgtagaaa acgaggaaca cgggaaggcg cagcagcgcg cctgagaaga gcggcagccc
     5581 cgccaacctt tgcgccacgc cgatagtgaa gggattggtg atggcggcag aaaacccgat
     5641 attagtggcc agtatgctca ttcccagacc taccagcgcg tcccaaccta atgagtagga
     5701 aagcgcgatc aataaaggca ccagtggaat gatctcttcc agcagcccga agaagccacc
     5761 cagcagcatg aaaaacaagg cgatcactaa aagcagcacg tagcgccgcg aacggaagcg
     5821 tttaacgatc ttccccagca aggcggtgag aataccgctt ttttccaaca ccgcgaatgc
     5881 cccgctgacc aggacaatga aaacaatgat ggtgatgatg atcaggctgt cttcgcccca
     5941 tagcacttcc acgggcgcgc tgaaccagcg ccagatcgga taatccgggc gttcgatgac
     6001 ctggtaagat gcaggatcga tcaggctgcg tccctccacc tggtaacgct ggtaactgcc
     6061 ggcggggatc acccgggtga gaatgcccgc agcaatcatc aataccagta aaatcacagc
     6121 ggattggata aaggcttttt ggctgatctg cgctccggct ttggattcca taggtgacca
     6181 tccttatgag gtgaagtctt tcataagtgt agacctggag atgacctgtg tcaaattggc
     6241 ggctaaaaaa gtggatgccg aggagaaagc gctggtatga ttatggatga atatgaccga
     6301 acccatagag tctgtcacca ccatagcggg gcgccgaata ccctaccgcc tggttcgctc
     6361 actgcgcgcc agccggatgc ggatgaccat taaagaaagc caggtgactg tgacattgcc
     6421 caggggggtt ccgccgagtg aagcggagcg ctttttacag cagaacgccg cctggctgtt
     6481 caaacaactg gataaaccag cgtcgcgcaa aaaaccctta ccggcgctgc cgaaggatgt
     6541 gatcctgctg cgcggcgaag ccacccatat ccaggtgatc gaagaaccca cccgctccac
     6601 gcgcatgcgg gtggatgaag caggcggcag gctggtagta cgggtgccgg ctggcacgcg
     6661 cgagtcgccg cggcggctgg tggtgccgtg gctcaaggcg caggcgcgcg gcacaattga
     6721 ggtggctgtc aagcagctgg cagtgcgcat gggcgtcact tacaagagtg tttccatccg
     6781 cgaccaacgc actcgttggg gcagctgttc gagccagggc gcgctttcat ttaattggcg
     6841 attggtgatg gtgcctcccg cggtgctgga atacgtggta acgcacgagc tcgcgcaccg
     6901 caaacagcag aatcattctc gggcgttttg gcaggtggtg gcgctttact ttccgcactt
     6961 taaggcagcc cggacgtggc tgcgcgcgaa tgctgccctg ctgcgaccca aagacacgat
     7021 atagaccctg aaacaggtat aataagttca atggagttca cccatcacac tgagtttccc
     7081 tatgaaatgg caaccgaatg gaacgcgttg ctggctgaaa gcgcgactca tgtgcctttt
     7141 ttacgctgcg agtacctggc ggattggtgg gcgacacggg gcggaggtga atggccggat
     7201 gattctctgc tggcaatcat caccgcccgc gaagatggcc gactgatagg tatcgctcca
     7261 ttcttcatca cctcgcacga ggataaaaaa aagctgctgc tgttgggcag tattgaaatc
     7321 agcgattatc tcgatgtcat ttgccgcgcc gaggaaatta accggttttt gcacggactc
     7381 ttgcgcttta ttcgggagca atttgtcgcc acagggcaaa tcaccgggct ggacctctac
     7441 aacatcatcg aggactcccc aacccttaag gcgctcgagg aatcagcgaa gaccctggac
     7501 ctggagatgc gcgtgcgcaa acttcaacac tcgccgttca tcccactgcc gggtaactgg
     7561 gaaacgtacc tggaaaggct tgaaaaaaag caacggcatg agatccgccg gaaaatgcgc
     7621 cgcgccctcg aaggggatgc gcaagtaaag ctttacttta ctcaagacga aggaaacctc
     7681 gaagatgata tggaggactt tctggagttg atggcgcagg acgaagccaa ggaagatttc
     7741 ctttcgccga tgatgcgcga gcagatgaaa tccgtcatgc gctgtgcctt tgcccaggac
     7801 tgcctgcagc tggcttttct tgaaatcggc ggacagaaag cggctgctta cctgagcttt
     7861 gattttctca accgcatctg ggtgtataac tccggtattg accggcgcta tatggaatat
     7921 tcaccgggtt gggtgctgtt gggttacttg ctgaaatggg ctaatgaaaa cgggcgcgaa
     7981 acctttgatt ttatgcgggg gaatgaagat tacaaataca agttcggcgc gttagaccgt
     8041 tttgtgatgc gcgtcagcct tgaattctaa gcaggacgta gataaaaagc gggtgcttga
     8101 acggcaaccc gcttttaatg cccctgattt ttgctcacat ttctccgctt gaccaacgct
     8161 ggataaggcg gtggaagaaa gataccatct tgtgcatgtt ttctaatgag atgcgttcgt
     8221 taattccgtg aatacgctca aggtcttttt tgtgtaacaa gaggggcgaa aaccgataca
     8281 cacgagaaga aatggcgtgg taattacgcg cgtcagtggc gccaagcatg ttgcttggtg
     8341 cgcaggcgat atcagtgaag agttcattga gcacgctgac gatatgctgg tacgccgggg
     8401 tatcggtagg agaaacggga gagggttccc aggcgtattc ggggagcggt tcgatgcgca
     8461 cacggctgtc attgatcact gattgaacct gctcagttac ctcggcgact gaatcacctg
     8521 gcagcagcct gaagttcacc accgcctcgg cgtgcgttgg cagcacgtta tcttttacgc
     8581 caccgtgaaa gagcgtgggc gcggtggtgg tatggatggc ggcggcggtt tcggcttgcg
     8641 ctgctagttg acggcgcacc agaccgccaa ataaccacag gttcgcaaaa gccacctgca
     8701 gcaacctggg ggcggccggg ctgagaccct tgaacattgg gtatacggta ttcaaccggt
     8761 aaggaaaggg gtgagattgc aggcggtcaa tggcgcgcgc cagaacaccg atcgcagttt
     8821 cggccgcagg ggtagaagaa tgaccgccgc tggcttctac ggtcagtttg agtgagaggt
     8881 agcctttttc agccacgccg attggcgcgg tatgtccctt aaagccaggc agtacaccat
     8941 ccagcaccgc attgccttca tccagaacgg cgtgcacgct tatccccttt tctttgagat
     9001 gtctgacgat cgctggagcg ccgtgcccca acacctcttc atcgtgtgaa aaagaaagca
     9061 agaccgtcct tacgggttga tagccctgct ccagcaggag ttcgaccgct tccattacag
     9121 cgatcagctg gtttttaata tccaaacttc ccctgcccca aatgaaaccg tctgcaattc
     9181 gcccggagaa aggcgggtgg gtccattgct ccagcgtatg ctcatcgata ggcaccacgt
     9241 cctggtgggc cataaagacc acgggttcaa gagcggggtc gctgccctgc caggtgtaca
     9301 gcaagctgaa gccctgcacg atttcgcggc tgagagaccc gtgaaggcgg gggtaggtgc
     9361 gttgcagcag atcatgcagc cgttcaaagt tttcggtgcg gtttttcgct gggtcttcat
     9421 gcgatacggt ttctatctgt atcgcagcag agagatgctc cgcaattttt tgcctgtcca
     9481 gtggtgggtc gggcagtttg gattgaaatg gtaatggctt gtgacccgcg gagcgaatca
     9541 gcaggaacgt acgggcggcg atgacagccg cgaggacgag aatcaaaaag accagtggaa
     9601 ggaagaattg catacgcacc tcatgtccag cggcaaaggt tattcctgca cgggtcggca
     9661 gtgatgacca gagcagttat cagggggttg aagatggctt tggcgcccag aaccccggca
     9721 atctgaaccg ccgggtgcta cgcgtgttcc atccaatttt ttatagttta atctcgttgc
     9781 tcaggatgaa gttttccaga cctttgataa gcgtttcttt gtcctgcagc atcgaggtga
     9841 ccacatcccc tatcgagata acgccaacga ctttcccttt gtctaacacc ggcaggtggc
     9901 ggatgtgctt atcagacatt tgcgccatgc aagtatcggt cgattgctcg gggcttacgt
     9961 aataaacttt ttcggtcatc acccgttgaa ccggtgtgtg ttcatgattc cctttcagaa
    10021 ttccccgcca ggcgtaatcc cgctctgaga aaatgcccac cactttttca tcctgcatta
    10081 ccagcacgga tccgattttc ttctcgctca tcagtttcaa acaatcaatg atggaagtct
    10141 cgggtgagac agagtaaacc tgggagcctt tctttttgag aatggatgca actgtagcca
    10201 taatcacctc cgtaacgtgt tgtcttgttg agcagtgcgt taataatagc ctatccttga
    10261 atgacttgtc aatgacc
//
LOCUS       DAOTLW010000051        10122 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_6938_length_10122_cov_11.859442, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000051 DAOTLW010000000
VERSION     DAOTLW010000051.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 10122)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 10122)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10122
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_6938_length_10122_cov_11.859442"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..669
                     /locus_tag="P5516_07520"
     CDS             <1..669
                     /locus_tag="P5516_07520"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRT92320.1"
                     /translation="NFTEFWNRWHITLSNWLRDYIYYPLAWSLRRRFHQQDHFFNIFL
                     PPMVTLLVSGLWHGLSWSLLVWGALHGLYLVLERLPSLGKAVVPVNKRPLWRQRLGTA
                     VTFLFASLAWVPFRMPLPAALQYWQGLLRWSMPDFIGLARTLVGWSPVSGWSQFDLPN
                     PILLLVLAAALLFDQLQHRAGTEEFMLNQPRWLQVLLTALLLLVATLAFFSDTTAPFV
                     YQGF"
     gene            743..2272
                     /locus_tag="P5516_07525"
     CDS             743..2272
                     /locus_tag="P5516_07525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92321.1"
                     /translation="MKNRIESVLLYLLPSVGAILWISIFFGVLMSGPRMVNADGDLGR
                     HITIGGYILDNHLVPLRDVFSHTMLGEPLTPHEWLAQVLFAQVHRLMGINGAVLLAAL
                     VIATAFWLVYRQTRAQSGSLLAAAMVSILSLLASSIHWLSRPHIFTFLLLALWMGVLY
                     QIKQGNTRRWWLMPLLMIAWVNLHGAFIAGFATWGLCAFGMLWERWFTRSEEREVPPA
                     GFWKSYLLGGGFSLLATLLNPAGLGIWGTSVGYVANRYLVDHTVEYQSPNFHEPITWL
                     LLLFIALLIVVLALRKRHTTTFQLFPAVAWLVMSLYSARNIPLFAIVAAPLLAKACVD
                     FLSGLELPEILRRRVEKLDAGLLKVDLRLKGIFWPVLSVVIVVIGFSAGMTFTRDGTA
                     NAYDPAKFPVGAVDWLEQNPQRGEVFNYFPWGGYLLYRQWPQCLVFIDGQTDFYGEAL
                     TREYQRVIGLEDGWGAVLDGHNVGWVILPVKEHAACALEQQDGWQVVYRDGTSVIIGR
                     K"
     gene            2355..3476
                     /locus_tag="P5516_07530"
     CDS             2355..3476
                     /locus_tag="P5516_07530"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT92322.1"
                     /translation="MAVEIREVTTIKGLKKFIRYPLTLYKGSPYYVPNLFADELNTLR
                     ADKNPAFEDARARYWMAYRENQAVGRVAAMIVPRHEEKWGEKYMRFGWIDFIDDAEVV
                     KALMDTVENWARQEGKLGVHGPLGFTDLDREGMLVEGFDEMTTLATIYNYPYYPQRLE
                     ELGYTKDVDWIEHEFTFSTQLEEKIANAAALIAQRTKTHLFQGKKNDLLGLAPQIFDV
                     INEAYRHLYGTVPLTEAQVKSYVNTYFGFANPDFIPLVMDENERLVAFGITFPSFSRA
                     LQKSKGNLFPFGFIHFLRALHKNDRADLYLVGVRDEYRGRGITALLMHQIFQTFKKHG
                     IRYIETNATLENNQDILAMWKYLDSRQHKRRRAYVKHLA"
     gene            3760..5169
                     /gene="radA"
                     /locus_tag="P5516_07535"
     CDS             3760..5169
                     /gene="radA"
                     /locus_tag="P5516_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HRT92323.1"
                     /translation="MPKTISQYVCQQCGKVAPRPLGKCPQCGGWDTMVEEVLQAPAAP
                     AKTHLRGLNDRSHPRRMAEIQGNAEERISLPIGEFARVLGGGVVPGSIVLVGGDPGIG
                     KSTLLLQMALEMAGDHSARKVLYVSGEESERQIKMRALRLTRQPGEKSAGFSDDLLLL
                     TETNLEAILEHIGALKPDLLIVDSIQTVYLPEMSSTAGSVSQVRECANRLRELAKSSG
                     MAVFLVGHVTKEGVIAGPRVLEHIVDTVLYLEGDRFQSYRLLRSVKNRFGATAEVGVF
                     EMREQGMVEVLNPSEAFLAERLVNAPGSAIAVTMEGTRPLLVEMQGLTSPSNLGNPRR
                     TPNGVDFNRLLLITAVLTRRLGLRLAEQDVFVNVIGGLQVNEPAADLAIAASIASSMK
                     DIPVKADLVLIGEVGLSGELRWVGQMNARLREAAKLGFKTALIPRRVRPGEPLPKEIE
                     VKEARSLREALGFALLTEK"
     gene            5197..5736
                     /locus_tag="P5516_07540"
     CDS             5197..5736
                     /locus_tag="P5516_07540"
                     /inference="COORDINATES: protein motif:HMM:NF039426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6391 domain-containing protein"
                     /protein_id="HRT92324.1"
                     /translation="MAELNKVLDWAPVSMIRRNHGLEHATLNLLAAEFPSCSFAGHSD
                     SKGFWIIGEVSTDKLLETVQQALKRMKAGESDLALHTNCGTNYVTTGLIAGSVAWLGT
                     LGNANSFSKKLNRWPLLVMLITGSLILAQPLGKKVQARISTSGDPGNLVVKQITRYER
                     KGPTLHRVLTTDILEITES"
     gene            5740..6762
                     /gene="holA"
                     /locus_tag="P5516_07545"
     CDS             5740..6762
                     /gene="holA"
                     /locus_tag="P5516_07545"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HRT92325.1"
                     /translation="MTDAHVHLLRGDDAFAISLQVKSIIASLGADFDSSMNYSRLDGK
                     SVSLEDMRLAVSTLPFFGGRRLVVIDDAISKVEKSRVPAFTAMLASAPPTTTLVIIVE
                     DTQKWRKVENEWTRVWETLTPAHWLVQWCSSHPQACIVDLGLPDAKNMDAWILNETKR
                     QEGKMEPSAAYELSRLTGIDTSIASQEIAKLLMHVDFQRPVTREDVLELVSDEGSVDV
                     FAMLDSLMEGKTRNAQAMMRRLLDDTPPEVVLGALAHRLRQLILLAEAMDSGEDARSL
                     ARKSGIFINKIESSRNAVRCIGISGLEELYHHLLEIDLQAKTSGTDLATNLELLVLKT
                     GHYFKK"
     gene            6791..7855
                     /locus_tag="P5516_07550"
     CDS             6791..7855
                     /locus_tag="P5516_07550"
                     /inference="COORDINATES: protein motif:HMM:NF012927.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase/NADH oxidase"
                     /protein_id="HRT92326.1"
                     /translation="MTHLFDPLKIKDIILRNRVGVSPMCQYSYTDGFSNDWQVIHLGA
                     RAVGGAGLVIAEATAVEARGRITPFDVGIWRDEHIDPLRRVVKVVKENGAVPGIQIAH
                     AGRKACVGQPWTGGRPVQEGDPLSWPVVGASPIAFSEEHQVPNELTVEEISAIRKRFV
                     EGAQRSLAAGFEWLEIHGAHGYLLQSFCSPLSNQRGDQYGGVLENRVRFLLEVVRDVR
                     RVWPERLPLTVRISGTEWVEGGWNVQDSLVLARLLKEEGVDLVDCSSAGNLEKAPVPT
                     DPGYQVHIAEAVRRGAGILTAAVGLITTAQQAEEIIHSGSADLVLLGREMLRSPYWAI
                     LAAKELGQAPPVPPQYLRAF"
     gene            complement(7900..8910)
                     /gene="galE"
                     /locus_tag="P5516_07555"
     CDS             complement(7900..8910)
                     /gene="galE"
                     /locus_tag="P5516_07555"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HRT92327.1"
                     /translation="MKYLITGGAGYIGSTICSALEDAGHVPVVLDSLITGREEFTRGR
                     IFYQADIADERAVTQLFREHPDIQATVHCAALIVVPDSVSSPYEYYQDNVSKSIEFFH
                     LLHRLGYDRVVFSSSAALYDVVPGFMVTEEAPLNANSPYSRTKLMMEMVLRDYCAAYG
                     MKGIALRYFNPIGADPKMRSGIHVQHPTHVVGKLVDTAQGRQSEFQITGTHYPTRDGT
                     GIRDYIHVWDLAQAHVNAVTDFDKVFERAGELPVDYLVINLGTGRGVTVRELVTAFEK
                     VYGQTINKKETGPRPGDVAGSYTNADKAYRLLHWKAQLSIEEGIADALKWGKLRENIL
                     KY"
     gene            complement(9001..9465)
                     /locus_tag="P5516_07560"
     CDS             complement(9001..9465)
                     /locus_tag="P5516_07560"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HRT92328.1"
                     /translation="MLGLIIGFSLLLTGVVFLIIGLVAAKRAKAAQSWPTIPGRVLSS
                     QVVEHESTDSEHGTTTVTYEPRVEYEYIVMGQAYTAKRIAFGANRFHYKKAEEIAARY
                     PVGAQVNVRYNPDKVKDATLEANAAGGKLFLILGGVFGAVGLVTMVISLFSL"
     gene            complement(9622..10092)
                     /locus_tag="P5516_07565"
     CDS             complement(9622..10092)
                     /locus_tag="P5516_07565"
                     /inference="COORDINATES: protein motif:HMM:NF023580.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3592 domain-containing protein"
                     /protein_id="HRT92329.1"
                     /translation="MQLRSIVFMLGFILLGCVFMFRAVSGRLKSKKAQAWPVVKGRVV
                     SSEVEEDRFRSATGKATIAFVPDISYEYQVNGKSYTSRTVIFGATTYDYLTASRICEK
                     FSVGSEPKVYYNPANPQDAVLLPQATEGLRSLIPGTFFIVTGIAIGVMGFLFPN"
BASE COUNT         2087 a         2869 c         2954 g         2212 t
ORIGIN      
        1 aatttcaccg aattctggaa ccgctggcac atcacccttt caaactggct gagggattat
       61 atttactacc cgctggcctg gtctctgcgg cggcggttcc atcagcaaga ccattttttc
      121 aacattttcc tcccgccgat ggtgacgctg ctggtaagcg gtttgtggca cggtctctcc
      181 tggagtttgc tggtgtgggg agccctgcac ggtctctatc tggtgctgga acggctgccc
      241 tcgctgggga aggctgttgt gcctgtcaac aagcgcccgc tctggcgcca acggctggga
      301 acagcggtga cctttctgtt cgcgtcgctg gcctgggtgc ccttccgtat gccgctgccc
      361 gccgccctgc agtactggca gggtttgctg cgctggagca tgccggattt catcggcctt
      421 gcccgtacgc tggtgggttg gagccctgtc tcaggctggt cgcaatttga tcttcccaac
      481 ccgatcctgc tgcttgtgct cgccgccgcg ctgctctttg accaattgca gcaccgcgcg
      541 ggcaccgagg aattcatgct gaaccaaccg cgctggttgc aggtgctgct gacagcgctg
      601 cttttgttgg tggcgacgct ggcgttcttc tcagatacga ccgccccatt tgtgtaccag
      661 gggttctgag cagaatggct tctgcgcgaa ttgttgaggt ttttatgctt acgatgaatt
      721 tccttagccc ggggtcaacg aaatgaaaaa ccgcatcgag tctgttttgc tgtacttgct
      781 gccctcagtt ggcgccatac tatggatcag catctttttt ggtgtgctga tgtctggccc
      841 gcgcatggtc aacgctgatg gtgacctagg caggcatatc accatcggcg gttatatcct
      901 cgacaatcat ctcgttcccc tgcgcgatgt tttttcgcac accatgctgg gtgaaccgct
      961 caccccgcac gaatggctcg ctcaggtatt gttcgcgcag gtgcatcgtt taatgggcat
     1021 aaatggcgcc gtcctgctcg ccgcgctggt gattgccacc gcgttctggc tggtttaccg
     1081 gcagacccgc gcgcaaagcg gttccctgct cgccgctgcc atggtgagca tactctctct
     1141 gctggcttct tccatccatt ggttatcacg cccccatatc ttcaccttcc tgttgctggc
     1201 cttgtggatg ggcgtgctct accagatcaa acaaggaaac acccggcgct ggtggctgat
     1261 gccgttgctg atgatcgcct gggtgaacct gcacggcgcg ttcatcgccg gctttgccac
     1321 ctggggattg tgcgccttcg gcatgctttg ggagagatgg ttcacccgca gcgaagagag
     1381 agaggttcct cccgcgggtt tctggaagag ttacctgttg ggcggcgggt tttcactgct
     1441 ggccaccctg ctcaacccgg ccggcctggg aatctggggc accagcgtgg gttatgttgc
     1501 caaccgttac ctggtggacc acacggttga gtaccagtct cccaattttc atgagcccat
     1561 cacctggctg ctgctgctct tcatcgcgct gttgatcgtc gttctcgccc tgcggaagcg
     1621 ccacacaact acttttcaat tgttccccgc ggtggcgtgg ctggtgatgt cgctttacag
     1681 cgcgcgcaac attccgctgt ttgccatcgt ggcggcgccg ttgctggcaa aagcctgcgt
     1741 ggattttctc tcagggctgg agttgcccga aattctacgc cgccgggttg agaagttgga
     1801 cgccgggttg ctgaaggtgg atttgcgctt aaagggtatc ttttggccgg tgttgagtgt
     1861 tgttatagtg gtgattggtt tttccgccgg aatgaccttc acccgcgacg gaacggccaa
     1921 cgcgtacgac cccgccaagt ttcccgtggg tgcggttgac tggttggagc aaaacccaca
     1981 gcgcggagag gtgttcaact acttcccctg gggcggctac ctgctctacc gccagtggcc
     2041 ccaatgcctg gtgttcattg atgggcaaac cgatttttac ggcgaggcgc tcacccggga
     2101 ataccagcgc gttattggcc ttgaagacgg ttggggggca gtgctggatg ggcataacgt
     2161 gggttgggtg attttgcccg taaaagaaca cgcggcgtgc gcgctcgagc aacaagatgg
     2221 ctggcaggtg gtttaccgtg atggaacctc cgtcatcatc ggcagaaaat aagttaggtt
     2281 gcacatgacc gtacaggaat tctgactgtg taccaaccgg cagatgctgg aatttagaac
     2341 caggagaaac accaatggca gtagagatca gagaagtcac cacaatcaag ggtctaaaaa
     2401 aattcatacg ctaccctctc accctttaca agggcagccc ttattacgtt ccgaacctgt
     2461 tcgccgatga attgaacacg ctgcgcgcgg ataagaaccc ggcgtttgaa gacgcccgcg
     2521 cgcgctactg gatggcctat cgtgagaacc aggctgtggg ccgtgtcgcc gccatgattg
     2581 tgccgcgtca cgaagaaaaa tggggtgaaa agtacatgcg ttttggctgg atcgatttta
     2641 tcgacgatgc ggaagtggtg aaagcgctca tggacacggt ggagaactgg gcgcggcagg
     2701 agggcaagct cggcgtgcat ggtccgttgg gttttaccga tctcgaccgc gagggtatgc
     2761 tggtggaagg cttcgacgaa atgaccacac tcgccaccat ctacaactac ccctattatc
     2821 cgcagcggct ggaagaactg gggtacacca aggatgtgga ttggatcgag cacgagttca
     2881 ccttctccac ccagctggaa gaaaaaattg ccaacgcggc cgcattaatt gcccagcgca
     2941 ctaaaacgca cctctttcag ggtaaaaaga atgacctgct ggggctcgcc ccgcagatct
     3001 tcgatgtgat caacgaggcg tatcgccacc tgtacggcac cgtgcccctc actgaagcgc
     3061 aggtgaagag ttacgtcaac acctatttcg gcttcgccaa ccctgatttc atccccctgg
     3121 taatggacga gaacgagcgg ctggtggcgt tcggtattac ctttccctct ttctcgcgcg
     3181 cgctgcaaaa gagtaagggc aacctcttcc cgttcggctt catccacttt ttgcgcgccc
     3241 tccacaagaa cgaccgcgcc gacctgtacc tggtgggcgt gagagatgag taccgcggca
     3301 gagggatcac cgccctgttg atgcaccaga tctttcagac cttcaagaaa cacggtattc
     3361 gatatatcga aaccaacgcc acgctggaga ataaccagga tatcctggcc atgtggaagt
     3421 accttgacag ccgccagcat aagcgccggc gcgcctacgt caaacacctg gcctgatcct
     3481 ttggcgtaaa aggcagcctt cgccagggga ggcgggcgcc gtgaccgctg cttttttacc
     3541 agcggttggc gttgttgcca tgcgctagta aaccgcgggg gcgattgcaa cttctttccg
     3601 gtaaaacctt cgtgatttga cggttgctgt tttgctttcg tcgtttctga aagtacaaaa
     3661 cctcaaatca gcagccaatc tgagtgtggg taatctccag gcgcaaaccg cgcgccaggt
     3721 tcgcgttctc caaacgggtg gattgagtat aatcaggtta tgcctaaaac catcagccag
     3781 tatgtatgcc agcaatgcgg aaaagtggcg ccgcgacctt tgggcaaatg cccgcagtgc
     3841 ggcggatggg ataccatggt ggaagaggtg ctgcaggcgc ccgcggcgcc tgccaaaacc
     3901 cacttacgtg gactgaacga tcgttcacat ccgcgacgca tggcagagat ccaggggaac
     3961 gccgaagagc gcatctccct gcccattgga gagttcgcgc gcgtgttggg cggcggcgtg
     4021 gtgccgggtt ccattgtcct ggtgggcggc gacccgggca tcggtaaatc cacgctgctg
     4081 ctgcaaatgg cgctggaaat ggcgggcgac cacagcgcca ggaaggtgct gtatgtatct
     4141 ggtgaagaat cagagcgcca gatcaaaatg cgcgccctgc gcctcacccg ccagccgggt
     4201 gaaaaatccg ctggcttcag tgatgacctg ttgctgctca ccgaaaccaa ccttgaagcc
     4261 atcctggagc atatcggcgc gcttaaacca gacctgttga tcgtggattc catccagacg
     4321 gtgtacctgc ccgagatgag ctcaaccgcc gggtcggtca gccaggtgcg cgaatgcgcc
     4381 aaccgtctgc gtgagctggc caaatccagc ggcatggcgg tgttcctggt gggtcatgtc
     4441 actaaagaag gcgttatcgc cggtccgcgc gtgctggagc acattgtgga cacggtgctc
     4501 tacctggagg gcgaccgttt tcaatcgtac cgcctgctgc gttcggtgaa aaatcgtttt
     4561 ggcgccaccg ccgaagtggg cgtgtttgaa atgcgcgaac agggcatggt cgaagtgctc
     4621 aacccctcag aagccttttt agccgaacgc ctggtcaacg ccccggggtc ggccatcgcc
     4681 gtcaccatgg aaggcacccg cccgctgctg gtggaaatgc aggggctcac cagtcccagc
     4741 aacctgggaa acccgcggcg cacccccaac ggggtagatt tcaaccgcct gctgctcatc
     4801 accgcggtgc tcacgcgccg cctgggtctg cgcctggctg aacaggatgt tttcgtcaat
     4861 gtgatcggcg ggttgcaggt gaacgagccc gcagccgacc tggcgatcgc ggcttccatc
     4921 gcgtcttcga tgaaagacat cccggtgaaa gccgatttgg tgcttattgg cgaggtgggg
     4981 ctttcaggag aactgcgctg ggtggggcag atgaacgccc gcctgcgcga agccgccaaa
     5041 ctggggttta aaaccgccct catcccgcgg cgcgtgcgcc ctggtgaacc actgcccaaa
     5101 gaaatagaag tgaaagaagc ccgttcgttg cgtgaagcct tgggcttcgc gctgcttacg
     5161 gagaaatgat acgttccatg acctcggagg tgatatatgg ctgaacttaa caaggtgctc
     5221 gattgggccc cggtatccat gatcaggcgc aaccacggcc ttgaacacgc cacgcttaat
     5281 ctcttggccg ctgaattccc gagttgttcg tttgccgggc actccgacag caagggcttt
     5341 tggatcattg gggaagtttc gactgataaa ttgctggaaa cggtgcaaca ggcgcttaag
     5401 cgtatgaaag caggcgagag cgatttggcg ctacacacca attgcggcac caattatgtc
     5461 accaccgggc tcatcgccgg ctccgttgcc tggttgggca cattgggcaa cgcaaattct
     5521 ttctcgaaaa aactcaaccg gtggccatta ttggtgatgc tcatcaccgg ttcgttaatc
     5581 ctggcccaac ctctcggaaa aaaggtgcag gcgcgcatct caacctcggg tgaccccggc
     5641 aacctggtag tgaaacagat cacgcgctac gaacgtaaag gtcccacgct gcaccgtgtg
     5701 ctcaccaccg atatactgga aatcacggag agctgaccct tgaccgatgc gcacgttcac
     5761 ctgctgcgcg gagatgacgc tttcgccatc tcgctgcagg taaagagcat catcgccagc
     5821 ctgggcgcgg atttcgactc ctccatgaat tattcacgcc tggatgggaa aagcgtctcg
     5881 ctggaagata tgcggttggc ggtatccacg ctgccttttt ttggcggcag gcggctggtg
     5941 gtaattgacg atgccatcag caaagtggag aagagccgtg tgcctgcctt caccgcgatg
     6001 ctcgcatccg cgccaccgac caccacattg gtgatcatcg tcgaagatac acaaaaatgg
     6061 cgcaaggttg aaaacgagtg gacgcgcgtt tgggagaccc tcacgcccgc gcattggctg
     6121 gtacagtggt gctcttcgca cccgcaggcg tgcattgtgg acctcggcct gccggatgca
     6181 aaaaacatgg atgcctggat cctcaacgaa accaaacggc aggaagggaa gatggaaccc
     6241 tccgcggcgt atgagttgag ccgcctcact ggtatcgata ccagtatagc cagccaggag
     6301 atagccaaac tgctgatgca tgtggatttc cagcgcccgg tgacgcgcga agatgtgctg
     6361 gaattggtct ctgatgaagg ttcggtggat gtgtttgcca tgctcgattc gttaatggaa
     6421 ggcaaaaccc gcaacgccca ggcgatgatg aggcgcctgc tggatgacac gccgccggag
     6481 gttgtactgg gcgctcttgc tcaccgcctg cgccagctta tcctgttggc tgaagcgatg
     6541 gacagcggcg aagacgccag gagcctggcg cgcaagtccg gcattttcat caacaagatc
     6601 gagagttccc gtaacgccgt gcgctgcatc ggcatttctg ggttggaaga gctgtaccac
     6661 cacctgctcg aaatcgacct gcaggccaaa acttcgggga ctgacctcgc caccaacctt
     6721 gagcttttgg tcttgaaaac agggcattat ttcaagaaat aaatctcctt ttagacctga
     6781 tgaggaaaac atgacccatt tattcgaccc attaaagatt aaagacatca tcctgcgcaa
     6841 ccgcgtgggg gtttcaccta tgtgtcagta ctcttacacc gacggctttt ccaatgactg
     6901 gcaggtgatt cacctggggg cgcgcgctgt tggcggcgcg ggactggtca tcgcggaagc
     6961 cacagcggtg gaagcgcgcg ggcgcatcac cccctttgat gtcggtatct ggcgcgacga
     7021 acacatcgac ccgctgcgcc gggtggtgaa ggtggtgaaa gagaacggtg ccgtaccggg
     7081 tatccagatc gcccacgccg ggcgcaaggc ctgcgtgggg caaccgtgga cgggaggccg
     7141 ccccgtgcag gagggagacc cgttgtcgtg gccggtcgta ggcgcaagcc caatcgcgtt
     7201 ttcagaagaa caccaggtgc cgaacgaatt gaccgtggaa gagatctccg ccatccgcaa
     7261 gcgctttgtg gaaggcgccc aacgcagcct ggcagcgggt ttcgagtggt tggagattca
     7321 cggagcgcat ggctacttgt tgcaatcctt ctgctcgccg ctttccaacc agcggggtga
     7381 ccagtacggc ggtgtgcttg aaaaccgcgt gcgcttcttg ctggaggtgg tgcgagatgt
     7441 ccgccgtgtt tggccggaac gcctgccgct gacggtgcgc atttcgggta ccgagtgggt
     7501 ggagggcggt tggaacgtgc aagactcgct ggtgctggct cgtttgctca aagaagaggg
     7561 ggtagacctg gttgattgtt catcggcggg caacctggag aaagcgcctg tacccaccga
     7621 cccaggctac caggtgcaca tcgccgaagc agtgcgccgg ggggcgggta ttctcaccgc
     7681 cgctgtcggg ctaatcacca cggctcagca ggctgaggag atcatccaca gcggcagcgc
     7741 tgacctggta ctgctggggc gggaaatgct gcgcagccct tactgggcca tcctcgctgc
     7801 caaagaactc ggccaggcgc cgcccgtgcc gccgcagtac ctgcgcgctt tttgataaac
     7861 ccataaaaca acggctggag gtgctccagc cgttgttatt taatacttga gaatgttttc
     7921 gcgcagtttt ccccacttga gggcatctgc aataccctct tcgatggaaa gttgcgcttt
     7981 ccagtgcagc agccgatagg ctttatcggc gttggtgtaa gaacctgcca cgtccccggg
     8041 gcgtggaccg gtctcttttt tgttgatcgt ttgaccataa accttttcaa aggcggtcac
     8101 cagctcacgc acggtcacgc cgcgcccagt gccgaggttg atgaccaggt aatcaacggg
     8161 cagttctccg gcgcgttcaa agaccttatc gaaatcggtt acagcgttca catgcgcctg
     8221 cgccaggtcc cacacatgga tgtaatcacg aatgccggtg ccgtcgcggg tggggtagtg
     8281 cgtgccggtg atctgaaatt ccgactgccg cccctgggcg gtatccacca gtttgcctac
     8341 cacgtgcgtg ggatgctgca catggatgcc gctgcgcatt ttcgggtccg cgccgatggg
     8401 gttgaagtag cgcagcgcga tgcctttcat gccgtaagcc gcgcagtaat ctcgcaggac
     8461 catttccatc atcagtttgg tgcgcgaata ggggctattg gcattcaaag gggcttcttc
     8521 cgtcaccata aagccgggca ccacgtcata aagcgcggcg gaagaactga acaccactct
     8581 gtcataaccc aggcggtgca ataaatggaa aaactcgatc gatttggaga cgttatcttg
     8641 ataatactcg taagggctgc tgacggagtc gggcacgacg atgagggcag cgcagtgcac
     8701 cgtggcctga atatcagggt gctcacggaa gagctgtgtg accgcgcgct cgtcagcaat
     8761 atccgcctgg taaaaaatgc gcccgcgcgt aaactcctcg cgaccggtga tcagtgaatc
     8821 cagcacgacc ggaacatgcc ccgcgtcttc aagcgcggaa cagatggtgc tgccaatata
     8881 accggcgcct ccagtgatga gatatttcat gcctttcctc caagagcggt aaccgggtaa
     8941 tgcaaaacag gcttagtata ccaacacctg ccgcccgcgc aggtttagtt ctagaaataa
     9001 ttagagagag aacaacgaga tgaccatcgt caccagcccc accgcgccaa agaccccgcc
     9061 gaggatgaga aagagtttac cgccagcggc attggcttcg agggtggcgt ctttcacttt
     9121 atccgggtta tagcgcacgt tcacctgtgc gcctaccggg taacgcgcgg cgatctcctc
     9181 cgctttttta tagtgaaaac gattggcgcc aaaggcaatc cgcttggcgg tgtatgcctg
     9241 gcccatcacg atatactcgt actcgacccg cggctcgtaa gtgaccgtcg tggtgccgtg
     9301 ttcactgtct gtgctctcgt gttcgacaac ctgcgagctc aacaccctgc cgggaatagt
     9361 cggccaggat tgcgcggcct ttgcgcgttt tgccgcaacc aggccaatga tgagaaaaac
     9421 gactccagtc aacagcaggg aaaaaccaat gatcaaccca agcataagaa ccctcctttt
     9481 tcaagttagc gcttgctgcc gcggggtttc ccagggcagg cggcttcaca accacacaac
     9541 gtgtgggtcg ctctcgttca tttcatcctc gcgccaatca tcctttttca cttacccacg
     9601 agcgtccatc ggttaaagtt tttagttcgg aaataaaaat cccatcacgc caatcgcgat
     9661 ccctgtcaca atgaaaaagg tgccggggat caacgaacgc agcccttcgg tggcctgcgg
     9721 cagcagcaca gcgtcttgcg ggttggcggg gttgtagtac accttgggtt cactgcccac
     9781 ggagaacttt tcgcagatgc gcgaagcggt gaggtaatcg taggtggtcg cgccaaaaat
     9841 caccgtgcgg ctggtatagc tttttccgtt cacctggtac tcataggaga tatcaggcac
     9901 aaaggcgatg gtggctttcc ctgtggcgct gcgaaagcgg tcttcttcta cttcggaaga
     9961 gaccaccctg cccttcacca ccggccaggc ttgcgccttt ttagatttca ggcgtccact
    10021 gaccgcccgg aacatgaaca cgcatcccag caagatgaac cccagcataa aaacgatcga
    10081 acgcagctgc atgccttcct cccgtaattg agaatggttt ta
//
LOCUS       DAOTLW010000052         9212 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_7680_length_9212_cov_10.618652, whole genome shotgun sequence.
ACCESSION   DAOTLW010000052 DAOTLW010000000
VERSION     DAOTLW010000052.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9212)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9212
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7680_length_9212_cov_10.618652"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="P5516_07570"
     CDS             <1..1203
                     /locus_tag="P5516_07570"
                     /inference="COORDINATES: protein motif:HMM:NF012697.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92330.1"
                     /translation="AMKETKVLDNKDIFCHELQLKMNQLAPVCAKMEPYEFRYCLNNI
                     TPEEESWSSVLLETPETIEGWISSAEFFESIQIKPRVEGAIVLDVTITRLTQMLFVGL
                     VTGEYAADWVHKWFYFDPRSFNFYARSFYLTDEIRHHLGGAPYRAFEQKQAAFELAHE
                     VGYQEFKAANEEIDRAYIETILALVEKKGTPIMLAIAGPTAAGKTEIVERLSARFAER
                     SQKIAAIELDNFLLDRDYREELNIGSFGARALHFDLLRSCVREILNKKTIFTPRYNFI
                     DGTSSHDLDGQRKPHGKPVQVDPADIVFIEGNSPFLLPGMAELVGIKCVYLTDDPVRL
                     KRKWRRDIDYRKKYDPYYLRNRFFKEQPPMAAKNYQPQLALCDIFVDTTHAALWVTPE
                     TRALLSPS"
     gene            1644..2861
                     /locus_tag="P5516_07575"
     CDS             1644..2861
                     /locus_tag="P5516_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HRT92331.1"
                     /translation="MNIQAEIKQRVAVRRQDIIQFMRDICAIPSMESMIGPVGERIQQ
                     EIRTLGYDEARFDLMGNTIGRIGNGPKVLVYDSHIDTVGVGDKETWPWDPFVGKVEDG
                     VLYARGACDEKGSTPGMIYGLSIARDLGLLKDYTVYYFGNMEEWCDGIAPNAFVEVDP
                     NVRPDFVVIGEPTRMNVYRGHKGRIELKVTASGRSAHAAVHYLGDNAVYKMISIINSI
                     RELDRRFRFGMGSHPVLGHASVVVTDAAVRTNSLNAVPDQFTIFLDRRITLNESHDEV
                     IDLIKGLIPDYLRDEIKVEELYYDTPSYTGFVFPLPKYFPAWLAEEKHPLVRAGQGTI
                     EDLWGEPRPLGTWEFSTNGTYWAGKAGIPSIGFGPGDEKDAHSPTEHVSLDDVVTATE
                     FYAYLPYKLQQRA"
     gene            3053..>4878
                     /locus_tag="P5516_07580"
     CDS             3053..>4878
                     /locus_tag="P5516_07580"
                     /inference="COORDINATES: protein motif:HMM:NF014325.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HRT92332.1"
                     /translation="MSRKPSLKQRIQYHTDNLMARGPQAMIGLLGVFSLIIILLSAFV
                     VTIGGRIFFPEGVDNLSFIEAFWLSLVRTLDPGTMGADEGWGFRLIMLILPTLGGVFI
                     ISTLIGVLSNMIQNKIEDLRKGRSLVLEENHTIILGWSSQVFTILSELVEANLNQVNP
                     SIAILAMKEKVEMEDEIRSRLRDTKNTRIICRSGSPIDPAALELVRPQHARSIIVIPP
                     EDQDPDNFVIKTVLAITNNPHRRAEAYIIVTQIRDEKNLQVVQMLGKKDRVCALLARD
                     IIARVTAQTSRQSGLSIVYTELFNFSGDEIYFKEEKALTGKRYRDALTSYATSSVIGL
                     FKADATTVQLNPPMDSLIQPGDQLICISADDDTILYSASPDTPAIESIQSAPQPISVK
                     KERLLLLGWNDYGATILRELDNYVGYGSQITIVADETFQEKVSTCTRLKNQTIEFHAG
                     DTTNRSLLDELKVEDYDHVIVLADTSLDFQEADARTLVTLLHLRDISLQDETPFSIVS
                     EMLDLRNRELATVTKVDDFIVSTHLISLMIAQLSENIHLMPVFTDMLNEKGAEIYLKP
                     VEEYIKTGVEVNFYTVVEAAARRNETAIGYRSASADEKSDSYG"
     assembly_gap    4879..4978
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <4979..5617
                     /locus_tag="P5516_07585"
     CDS             <4979..5617
                     /locus_tag="P5516_07585"
                     /inference="COORDINATES: protein motif:HMM:NF012804.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HRT92333.1"
                     /translation="LIVKTAVELVNKGYLSATGGNISVRIPSHQALAVTPSNLDYLHM
                     EVSDVCVLDLDLKPLAGERKPSIESAMHAAIYKTRPDVNAILHTHQAYPSALALIGKP
                     IPALFDEQVRFLGREVNIIPYQPSGTGLLVEVVVKHVNDHHNAYLMANHGALVFGEDM
                     VRAVGNVAVLDKCALAYLLTLCAGEKVSFIPVAARETLFAQLRKDQEKYGLD"
     gene            complement(5698..6075)
                     /gene="rplL"
                     /locus_tag="P5516_07590"
     CDS             complement(5698..6075)
                     /gene="rplL"
                     /locus_tag="P5516_07590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HRT92334.1"
                     /translation="MADMEKLVEQLSGLTVLEAAELVKMLEEKWGVSAAAPVAVAAGP
                     AVAAAAEPEEEKTEFDVVLKDAGPKKIEVIKVIRALTNLGLVEAKNMAETAGSKVLSG
                     VGKEAATDAKAKIEAAGGVVELA"
     gene            complement(6148..6675)
                     /gene="rplJ"
                     /locus_tag="P5516_07595"
     CDS             complement(6148..6675)
                     /gene="rplJ"
                     /locus_tag="P5516_07595"
                     /inference="COORDINATES: protein motif:HMM:NF000955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HRT92335.1"
                     /translation="MAFTKKQKGEMTAQYEKWLEESKAMFVLEYVGLKMKEVDALRAK
                     AREAGSEVHLVKNTLFVRALQSGGYTVPEFFEGTSLVGFAFNDPPALAKVFADFIKGN
                     ETRKVKGGFLGKVIISETQVKALADLPPLPVMQATLLGVISAPATKLVRTLAEPARSM
                     AAVVKAYSEKAPVAA"
     assembly_gap    6977..7076
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<7077..7261)
                     /locus_tag="P5516_07600"
     CDS             complement(<7077..7261)
                     /locus_tag="P5516_07600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HRT92336.1"
                     /translation="MAKHGKKYLEALTRVNKEQTYTPLEAVALAKETAYTKFDSTIEV
                     HLRMGLDPRQADQQVRDV"
     gene            complement(7311..7739)
                     /gene="rplK"
                     /locus_tag="P5516_07605"
     CDS             complement(7311..7739)
                     /gene="rplK"
                     /locus_tag="P5516_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HRT92337.1"
                     /translation="MSKKLKAIVRLQIQAGKANPAPPIGPALAGHGINIMAFCKEYNA
                     RTQNQVGEIIPAEITVFTDGSFTFILKSPPASELLKKAAGVPIGSKAPNREKVGKITR
                     AQLREIAEIKMKDMNALDVEGAMRQIEGTARNMGITIVEN"
     gene            complement(7811..8317)
                     /gene="nusG"
                     /locus_tag="P5516_07610"
     CDS             complement(7811..8317)
                     /gene="nusG"
                     /locus_tag="P5516_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018286740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HRT92338.1"
                     /translation="MVHCYSGYENKVRYNLEQRIESMGMKDKIFDVVIPTQEEIEVRD
                     GKRRTVERHVFPGYVLVNMILTEESWYVVRNTPGVTGFVGMGNDPTPLRPEEVQQILR
                     RMEAEAPMVKVTFKIGERVRIVDGPFNDFRGIVSEIDMEKTKVRVMVNFFGRETPVEL
                     DFLQVEKA"
     gene            complement(8539..8751)
                     /gene="secE"
                     /locus_tag="P5516_07615"
     CDS             complement(8539..8751)
                     /gene="secE"
                     /locus_tag="P5516_07615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HRT92339.1"
                     /translation="MADKNQKVKKENRFKRWWRETVGELRKVTWPTPREAWRLTKIVL
                     LVMVIMGAVLGGLDFMFTKLVGLVLG"
     gene            complement(8814..8889)
                     /locus_tag="P5516_07620"
     tRNA            complement(8814..8889)
                     /locus_tag="P5516_07620"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(8854..8856),aa:Trp,seq:cca)
     gene            complement(8930..9079)
                     /gene="rpmG"
                     /locus_tag="P5516_07625"
     CDS             complement(8930..9079)
                     /gene="rpmG"
                     /locus_tag="P5516_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001265708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HRT92340.1"
                     /translation="MRPVITFACDDCKERNYTSEKNRRNDPTRMELKKYCPRCKKHTL
                     HRETK"
BASE COUNT         2102 a         2388 c         2319 g         2203 t
ORIGIN      
        1 gccatgaaag agaccaaagt gttagacaat aaggatattt tttgccatga gctgcagtta
       61 aagatgaacc agctggctcc agtttgcgcc aaaatggaac cttacgagtt ccgctactgc
      121 cttaacaaca tcacgccaga agaagaaagc tggtcatcgg tattgctgga aacgcctgaa
      181 accatcgagg ggtggatcag ctcggcggaa ttttttgaaa gtattcagat caaaccacgg
      241 gtcgaaggcg cgattgttct ggacgtgacc atcacgcgtt taacccaaat gttgttcgtt
      301 gggttggtga ccggtgaata cgcagccgat tgggtgcata aatggtttta ttttgatcca
      361 cgcagtttta atttttacgc tcgttcgttc tatcttaccg atgaaatacg ccatcacctt
      421 ggcggtgctc cttaccgcgc cttcgagcag aagcaggctg cctttgaact ggcacacgaa
      481 gttggctacc aggagtttaa ggcagccaac gaagagatag accgggctta catcgaaacc
      541 atactggcat tggtagaaaa aaaaggcacg ccaatcatgc tggcaattgc tggtccgacc
      601 gcggcgggaa aaacagagat cgttgagcgc ctgagcgcac gattcgcgga gcgatcgcaa
      661 aagatcgcgg ctattgagct ggataacttc ctgctggatc gtgattatcg cgaagagctc
      721 aatatcggct cttttggggc tcgcgcgctg cattttgatt tattgaggag ttgcgtcaga
      781 gaaattctca ataagaaaac cattttcacc ccgcgttaca actttatcga tggcacatcc
      841 agtcacgatc tggatggaca acgaaagccg catggcaaac cagtgcaggt tgaccccgcc
      901 gacattgtgt ttatcgaagg aaactcgcct ttcttgcttc caggcatggc tgaactggtg
      961 ggaattaaat gcgtatacct gacggatgac cccgtgcgtc tcaagcgtaa atggcgccgt
     1021 gacattgatt accgcaagaa atacgacccg tattatctgc gcaaccggtt tttcaaggaa
     1081 cagcccccca tggcggctaa aaattatcag ccccaactgg cgctgtgcga tatctttgtg
     1141 gatacaacgc acgctgctct gtgggtaacc cctgaaacca gagcattgtt atcgcccagc
     1201 tgaaaacctg ccgttccccg agtgtgaggt tgtgataaag aatggcgtct ttcgaggaac
     1261 cgtcgaaaca tgcccgtttg agcggctccc gctcaatgta tcggttccgt gaggtttact
     1321 cgcctgcgcc aagcacttga accgccaggt tagctagaac agacatcgac gattgggcgc
     1381 aaaaaaaaag aacttattga acacgaaacg cggcgtcacc gaagaaacca ggccttgatt
     1441 caagccagac cacacaatgg aagcagcctg agcctgcgtg ttttaccagt tcttcgggga
     1501 ttcattgact attgtggcca taagtgtaca ataaaaggcg agccgggcag gcgcgctgaa
     1561 caggtcttca ggcagcctgc tcactgtgaa agataaggtt aaccggtcta agcgggtcga
     1621 tatcgcttct gtgagaggag aaaatgaaca ttcaagcgga gattaagcaa cgggttgcag
     1681 tcaggcgaca ggacatcatc cagtttatgc gcgatatttg tgccatccct tcaatggaat
     1741 caatgatcgg cccggtcggc gagcgcatcc aacaggagat acgtacgctg gggtacgacg
     1801 aggctcggtt tgacttgatg ggcaacacga ttggacgcat cggcaatggc cccaaagtgt
     1861 tggtatatga ttcgcacatc gacaccgtgg gtgttggcga caaggaaacc tggccctggg
     1921 acccctttgt cggcaaggtg gaagacggcg tgctttacgc gcggggcgcc tgtgatgaga
     1981 agggcagcac ccccggcatg atctatggtt tgtccattgc ccgcgacctg ggcctgttaa
     2041 aggattacac ggtctattat tttggcaaca tggaagagtg gtgcgatgga atcgccccca
     2101 acgcctttgt tgaagtcgac ccaaatgtac gtcctgactt tgtggttatc ggtgaaccca
     2161 cgcggatgaa cgtgtatcgc ggacacaaag ggcgcattga gctcaaagtg accgcttcag
     2221 gccgctcagc gcacgccgcc gtgcactacc tcggcgataa tgcggtttac aaaatgattt
     2281 ccatcattaa ttcaatccga gagctcgacc ggcgttttcg ctttggcatg gggtcacacc
     2341 cggttttggg tcacgcgtcc gtcgtcgtca cagacgcagc ggtgcgcacg aattcattga
     2401 acgcggtgcc cgatcaattc acgattttcc ttgaccgacg catcacattg aacgaatcgc
     2461 atgatgaggt gatcgacctg atcaaaggat tgatcccaga ttatttgcgg gatgagatca
     2521 aagtggaaga gctgtactac gacacgccat cttataccgg gtttgtgttt ccgctgccca
     2581 agtatttccc ggcatggctc gcggaagaaa agcatccgct ggtgcgagca ggtcagggaa
     2641 caattgaaga cctgtggggt gaaccccggc cgcttggcac ctgggaattt tcaaccaacg
     2701 ggacttactg ggcaggaaaa gccgggattc cctccattgg tttcggcccc ggtgatgaaa
     2761 aagatgcgca ttctcccacg gaacacgtat cgctggacga tgtggtaacc gccaccgagt
     2821 tctacgcgta cctgccgtat aaacttcaac aacgtgcctg agccagcctt cccaacggat
     2881 aatcaagcac gattctcacc tgcccgggtt ccctttccgg gcagtgtctt ttctaccagg
     2941 ctcacctcgc tattcgaatg aggagatgtt tcaatctgta gtatagtaaa tgaaagtcaa
     3001 ccgtttacca ggtcatggcc gcgagacccg ctgtggtagg aaggagattc acatgtccag
     3061 aaaaccatcc ctgaagcaaa gaattcaata ccatacagat aacctgatgg cgcgcggacc
     3121 acaagccatg atcggtttgt tgggggtgtt ctccctcatc atcattctct tatccgcgtt
     3181 cgtcgttacc attggcggga gaatcttctt tccggaggga gttgataatc tatcgttcat
     3241 tgaagccttc tggttgagcc tggtgcgaac ccttgacccg ggcaccatgg gagcagacga
     3301 ggggtgggga ttccgcctga tcatgctgat cctccctacc cttggcgggg tgttcatcat
     3361 cagcaccttg atcggcgtcc tcagtaacat gatccagaac aagattgagg atttgcgtaa
     3421 aggtcgctcg ctggtattgg aagaaaacca caccatcatc ctgggctggt cttcacaggt
     3481 gtttacgatt ctgtcagagc ttgttgaggc gaatctgaac caggtcaacc cttctatcgc
     3541 cattctggcc atgaaagaga aggtggagat ggaggatgaa attcgttctc gcctgcgcga
     3601 caccaaaaac acgcgcatca tttgtcgcag cggctcgccc atcgaccccg cggcgctgga
     3661 gctggttcga ccgcagcacg cgcgttcgat tatcgtgatc ccccctgaag accaggaccc
     3721 cgataatttt gtgataaaaa cggttctggc aatcaccaat aacccccatc gtcgcgcaga
     3781 agcctacatt attgttaccc agatacggga tgaaaaaaac ctgcaggtgg ttcaaatgct
     3841 cggaaaaaaa gaccgggtat gcgcgctgtt ggcgcgggac attatcgcca gggtgaccgc
     3901 gcagacttcc cgtcaatcag gtctatccat tgtctacacc gagctgttca atttttcggg
     3961 cgatgagatt tacttcaagg aggaaaaagc gcttacaggt aaacgctacc gcgatgcgct
     4021 gaccagttac gcgacctcat cggtgatcgg gttattcaaa gcagacgcaa caaccgtcca
     4081 gctgaatccg ccaatggact cgcttatcca gccgggtgac caactaattt gtatctctgc
     4141 cgatgacgac actatccttt acagcgcgag ccctgatact cctgcaattg agagcatcca
     4201 gtcagcacct cagcccatat cggtgaaaaa ggagcgtctt ttgctgctgg gctggaatga
     4261 ttatggagcc accatcctgc gggaattgga taattatgtt gggtatgggt cgcagataac
     4321 catcgtggcg gacgagacct ttcaagaaaa ggtgagtacc tgcacgcgcc tgaagaacca
     4381 aacgatcgaa ttccacgcgg gagataccac gaaccgcagc ctgctggatg aactgaaggt
     4441 cgaagactat gaccatgtga tcgtgctcgc tgatacctct ttagatttcc aggaggctga
     4501 tgcgcgaaca ctggtgacac tgctccatct acgggatatt tctttgcaag acgaaacccc
     4561 attttcgatt gtcagtgaaa tgctggatct acgcaaccgc gaactagcca cagttaccaa
     4621 ggtggatgac ttcattgtga gcacccactt gatttcgttg atgatcgctc agctctctga
     4681 aaacatccat ctgatgccgg tgttcactga tatgctcaat gaaaaggggg ctgagattta
     4741 cttaaaaccg gttgaagagt acatcaaaac cggagtagag gtgaattttt acacggtggt
     4801 ggaagccgcg gcgcgaagga atgaaaccgc cattggctac cgcagtgctt cagccgatga
     4861 aaaatccgat tcgtatggnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4921 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnntt
     4981 gattgtgaag accgccgtgg agctggtcaa caaggggtat ttgagcgcca ctggcggaaa
     5041 tatttcagtg cgcatcccgt cacaccaggc gctggcggtc acaccctcca acctggatta
     5101 cctgcatatg gaggtcagcg atgtttgcgt gctcgatctc gaccttaaac cgcttgcagg
     5161 tgagcgcaag ccctccattg aaagcgccat gcacgccgcg atctataaaa ctcgaccgga
     5221 tgtgaacgcc atcttgcaca cgcaccaggc ttaccccagc gcgctggcgc tgatcggaaa
     5281 accaatcccc gcgctgtttg atgaacaggt acgctttctt gggcgcgaag tcaacatcat
     5341 cccttaccag ccatcgggca ccgggttgtt ggtggaggtg gtggtgaaac acgtgaacga
     5401 ccatcacaac gcttacctca tggccaacca tggcgcgttg gttttcggtg aggatatggt
     5461 ccgcgcggtg ggaaatgtgg cagtgctgga taagtgcgcg ctggcgtatc tattgaccct
     5521 gtgcgccggc gaaaaggtca gcttcatccc cgttgccgcg agagaaaccc tttttgccca
     5581 gttacgtaag gaccaggaaa agtacgggtt ggattgagca cggtgggtaa agacaaccat
     5641 taagcggatc aaaaaaacct cccctcggga ggtttttttg atgtttttca agtgaaccta
     5701 ggcgagttcg accacgccgc cagcggcttc gatcttggct ttggcgtcag tagcggcttc
     5761 tttgcccact ccactgagca ccttggagcc agcggtctca gccatatttt tggcttccac
     5821 caggcccagg ttggtgaggg cgcggatgac tttgatgacc tcgattttct tcgggccagc
     5881 gtccttgagg acaacgtcaa actcggtctt ttcctcttca ggttcagcgg cggcggccac
     5941 agcgggtccg gcggcaacag ccacaggcgc ggcggcggaa acgccccact tctcttccag
     6001 catttttacc agctcggcag cttccaaaac ggtcagaccg ctcagctgct caacaagttt
     6061 ttccatatca gccatttcta ttactccttc gtttgattgt ttcgtgttta tcgggcaatt
     6121 cgtttacccg gaggttaagc cattcgatca ggcagcgaca ggcgcttttt ctgagtaggc
     6181 tttgacgaca gccgccattg aacgggcggg ttctgccagc gtgcgtacaa gcttggttgc
     6241 gggtgcggaa atcacaccca acaatgtcgc ctgcatcaca ggcagcggcg ggagatctgc
     6301 cagggctttc acctgggttt ccgaaataat gactttcccc aggaaaccgc ctttcacttt
     6361 gcgggtctcg tttcctttga taaaatcagc gaaaactttc gccagcgccg ggggatcatt
     6421 gaaagcaaaa ccaacgagag aagtgccttc gaaaaattcc ggaacggtgt acccaccaga
     6481 ttgcaacgcg cgcacaaaga gcgtgttctt caccaggtgg acttcacttc ctgcttcgcg
     6541 ggctttggcg cgcagcgcat ccacttcctt catttttagg ccaacatact ccagcacgaa
     6601 cattgccttg ctttcttcca gccatttttc atattgggca gtcatttcgc ccttctgctt
     6661 ctttgtaaag gccaatgaaa aatacctcct tccttataaa aataaatcag tctttgtccg
     6721 gttcttgccg cacaaagact gattccaatc cccctcaggg gagggttgtc gggattcaat
     6781 cttcacctcg gcaggatatt gagtctttgc agacaccggc tatctttggt gaacgctgga
     6841 acacatacca gcggaggata ttataacact atttttcgag tgcgatgttc atcgcctgca
     6901 acgggtccac cttaactcca ggacccatag tagcggcgac agtcactttc tttacatatg
     6961 aacccttggc gccagcnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     7021 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnacat
     7081 cgcgaacctg ctggtcagcc tggcgcgggt ctaaccccat gcgtaaatga acttcaatgg
     7141 tggaatcgaa cttggtataa gctgtttctt ttgccaaggc aactgcttca agtggagtgt
     7201 aggtctgctc tttattgacc ctggtaagtg cctccaggta tttcttaccg tgtttcgcca
     7261 tttcttctcc ttcgtggtgc aaacgggtgt ttcaacaccc tcccacctag ttaattctct
     7321 acaatggtga tgcccatgtt gcgggcggtg ccttcaatct gccgcatggc tccttcgacg
     7381 tccagcgcgt tcatatcttt catcttaatt tcagcaatct cgcgtaactg ggcacgggtg
     7441 atctttccga ccttctcgcg gttgggcgcc tttgagccga tgggtacgcc tgccgctttt
     7501 ttcagcagct ccgaagccgg gggtgatttt aagatgaatg tgaatgagcc gtctgtgaaa
     7561 acggtgattt ctgcagggat gatttcgccc acctggttct gcgtgcgggc gttatattcc
     7621 ttgcagaatg ccataatatt aatcccgtgc ccggcgaggg cggggccaat agggggcgca
     7681 ggattggctt tgccagcctg aatctgcaaa cgtacgattg cttttaattt ctttgacact
     7741 ttttttctcc ttggtggtta tagcgggtga tatttgggac accctcccac aaacaggtca
     7801 gcagacctgg ttatgccttc tccacctgca aaaaatccag ctccacaggg gtttcgcggc
     7861 caaaaaagtt caccatcacg cgaaccttgg tcttttccat atcgatctct gaaacgatac
     7921 cgcggaaatc gttgaacggt ccatccacaa tacgcacgcg ttcaccaatc ttgaaggtta
     7981 ccttcaccat tggcgcttcg gcttccatgc ggcgtagaat ctgctgcact tcttcagggc
     8041 gcagcggggt gggatcgttg cccatcccca cgaagccagt gacgccaggc gtgttgcgca
     8101 ccacatacca ggattcttcg gtcaagatca tattgacaag cacataaccc gggaaaacat
     8161 ggcgttccac cgtgcggcgt ttgccatctc ttacttcaat ttcttcctgg gtggggatga
     8221 ccacatcgaa gatcttatcc ttcatcccca tcgactcgat gcgctgttcc aggttgtaac
     8281 gtactttgtt ttcataacct gaatagcagt gcaccacaaa ccaggcgcgt tcctgcgaac
     8341 catccgttgt gaggtcgccc ggtcgttctt cgtctttgac ctggctggaa gggggtagtt
     8401 cttcagggac ggcaggttga gatgcgtcta attcacccga agggcttaat tcttcaggca
     8461 ggttttcgtc cagctcatcc tggttgaaat cgtcctgcgt gtccataaac accaaacctt
     8521 aatgtgccca ggatagggtt atcccagcac caaaccgaca agttttgtaa acataaaatc
     8581 caaaccgccc aggacagcgc ccatgatgac cattaccaga agcacgatct tggtcaggcg
     8641 ccaggcttca cgcggggtag gccaggtcac tttgcgcagt tcaccaaccg tctcgcgcca
     8701 ccagcgcttg aaacgatttt ctttcttgac cttctgattc ttatccgcca tgaccaactc
     8761 cttcttttca gggtaaaacg ccgccatcac gacggcggtt acgtctcact ttttggcagg
     8821 ccaggcagga atcgaacccg caacctcctg ttttggagac aggcactctg ccaattgagc
     8881 tactggcctg tacaggcagg gcggaaatcc gccctgccga attacagcgc tatttcgtct
     8941 cgcggtgcag cgtgtgtttt ttacaccgcg ggcagtactt cttcagttcc atccgggtcg
     9001 gatcgttacg gcgattcttt tcggatgtgt agttgcgttc tttacaatca tcgcacgcga
     9061 acgtaattac cggtctgata tctttctttt ttgaagccat atttcactct tgcagccgac
     9121 tttgaccggc tgacgctcct tttatttcga ttaagcgatg atttttgtga tcctgccggc
     9181 gccaaccgtc agtccacctt cacgaatcgc ga
//
LOCUS       DAOTLW010000053         9142 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_7730_length_9142_cov_10.964235, whole genome shotgun sequence.
ACCESSION   DAOTLW010000053 DAOTLW010000000
VERSION     DAOTLW010000053.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 9142)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 9142)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9142
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7730_length_9142_cov_10.964235"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /gene="ricT"
                     /locus_tag="P5516_07630"
     CDS             complement(<1..662)
                     /gene="ricT"
                     /locus_tag="P5516_07630"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HRT92341.1"
                     /translation="MMETPSSQLIVGVRFSKVGKIYNFDATKIQNIQRGDVVVVETSR
                     GWQLGEIITLSTPESQNADSTWKPIDRLATPKDLIQRQFWQNKEGEVIEQCASRAKEL
                     KLRGIKIIASEYSFDGTRLNILFSSENEEKVELKSLRADMQRQFAPAQVELRQIGPRD
                     VAKIMGGMGACGLESRCCTRFITEFSSISIKMAKEQGISLTPTEITGMCGRLRCCLIY
                     EY"
     gene            complement(659..1735)
                     /locus_tag="P5516_07635"
     CDS             complement(659..1735)
                     /locus_tag="P5516_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HRT92342.1"
                     /translation="MKLTVEMVTDALRNVIEPELHKDLVSLKMVENIQVAGESVELTV
                     VLTTPACPLSKKIEKEVKEAVLAIPGVSSVKVNMTARVPSDGKNREVLSLPVKNVVAV
                     ASGKGGVGKSTVAVNIAVILAQKGASVGLLDADIYGPNIPTMMGVDLLPPPEDGRLVP
                     AKAYGVKLMSIGFMVRKGQPLIWRGPMLHSAIRQFLADVNWGELDYLIIDLPPGTGDA
                     QLSLMQHVPLSGGVIVTLPQQVSLEDAERGLQMFTEMNVPVLGIVENMSYLPMPDGTK
                     MDLFGNGGGRKMAERLGVPFLGEIPVEPAVRIGGDSGKPIALSDPTSAAAQSLTRIAE
                     AMAAQISIAALTGSPAGEPRQGNE"
     gene            complement(1732..2070)
                     /locus_tag="P5516_07640"
     CDS             complement(1732..2070)
                     /locus_tag="P5516_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92343.1"
                     /translation="MLNSDTEILFPIRVIPSLRNLRGEEWQALIDRLTTAEAEEIGQG
                     AFSLMMVRLGGCAGCNADSFRAMRGCTHCAQLTVKRFKGSDDDLLQLYRDTLEEVIGH
                     LAKYELIEEQ"
     gene            complement(2082..2651)
                     /locus_tag="P5516_07645"
     CDS             complement(2082..2651)
                     /locus_tag="P5516_07645"
                     /inference="COORDINATES: protein motif:HMM:NF024247.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRT92344.1"
                     /translation="MNGTGIIKGLAVTIRHFFSTYFADQFGLGRAKAKRGTMMAEGFF
                     TVQYPEESVTVPEEFRVIPFLVYTIEEDGQKKYRCTACGICAKVCPTQCIWITRMKNE
                     ETGKPVPESAEFYIDIDKCMNCGSCAEFCPFDAIKMDHDFRIASYTRGESHIYNKERL
                     GKPLEYYAKIRPTNYAQEEAERAAKAQAG"
     gene            complement(2664..4412)
                     /locus_tag="P5516_07650"
     CDS             complement(2664..4412)
                     /locus_tag="P5516_07650"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HRT92345.1"
                     /translation="MSELTVLHPEYEALLQRFAPNVEPDTRPGFSGIVVLPASLIEVV
                     TYLKEQMGFDYLSSVTAVDQYPDDQFDVIYHLYKTIGGEGLELKVFTTRENPVVPSVT
                     QLYPGADFQEREIWDMFGIRFSGHYDLRRLLLWEGFSGFPLRKDWHEPLFEEEVKPYK
                     SRWPEGRAFHKEQTNPFHDNVQYPKEFDPESVSTDADESLYKALTANLANDEDGLHTD
                     RMVVNIGPQHPSTHGVFRMSAVLEGETVVALKPVLGYMHRNHEKIGERNTFLQNMPFT
                     DRLDYLSSMSNNFGYAITVEKLLGLPPPERGEFLRVMMAELTRIASHTMSIGFLLNDL
                     GAFFTPSLYALEERELILDIFESVAGSRMMCNYFRFGGVVRDISAADFAKVKYLVEER
                     LPRKIDDLDRYLTDNEIVRSRCEGVGVLTAEEAIACSAAGPILRASGVPYDIRRADPY
                     SIYDRFEFDVCYRNHGDVYDRYLVRLDEIRQSIRILKQVIEQIPPEGPVMNGKVQYLV
                     KVPPGESYGRVEGPRGELGFYVVSDGKVNPKRYHVRAPSLINLTGLEKMCVGGKIADA
                     VTILGSVDIVLGEVDR"
     gene            complement(4580..5074)
                     /locus_tag="P5516_07655"
                     /pseudo
     CDS             complement(4580..5074)
                     /locus_tag="P5516_07655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000236330.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
     gene            5168..5722
                     /locus_tag="P5516_07660"
     CDS             5168..5722
                     /locus_tag="P5516_07660"
                     /inference="COORDINATES: protein motif:HMM:NF013209.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HRT92346.1"
                     /translation="MNTFTKIKNYLEAHASATVSQLSRDLNLTKADIHYHIRILLKNG
                     EIAVLPDSYQAGAGRPARQFVRLRSAPDALARFLIDHLINLHLQNKDQQSSREALAEY
                     LSKCLLEQYQHFEVQHASPAVRLAKIVKNLSTFGIHLRWLAGKDGPMVSLEKEELSTL
                     IDDPVLVEAIVRDLLLRIQKMFIK"
     gene            complement(5745..7586)
                     /gene="glmS"
                     /locus_tag="P5516_07665"
     CDS             complement(5745..7586)
                     /gene="glmS"
                     /locus_tag="P5516_07665"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HRT92347.1"
                     /translation="MCGIVGYIGERDATPLILGGLKKLEYRGYDSAGLAVLKNGQIEV
                     RRNAGKLVNLEMNVCEQPISGTLGIGHTRWATHGEPSARNAHPHLSGSGEVVVVHNGI
                     VENFIELKEELVTEGVVFQSDTDTETIVHLIEKFLSVTGDLESAARKALGQLKGAHGI
                     VVFTSREPDKIIAARIGNAGGVVIGLGEGENFIASDIPAILEHTRKLIFLESRQMAVV
                     TREDVHISTIEGTVIHPQVSSVAWDPVSAEKGEYRHFMQKEIHEQVRSLTDTLAGRVN
                     FKEGTIRLPQLNLTVEKAKEVEKIVLTACGTASHAGMVGRILIERIAGIPAALEIASE
                     FRYSDPIVNDKTILIAISQSGETADTLAAMEEGRRKGATIWSIVNAIGSQAMRVADGS
                     ISMQTGPEIGVASTKAFTAPLVDLYMLAILLADLRGDLSVQERKKLVEDLRLIPELAG
                     RCLDREGEVEETAYLLRESKDMLYLGRGINMPIAYEGALKLKEISYIHAEAYPAGEMK
                     HGPIALIDENMPVVAIAIKDPWYEKMISQIEQAKARGGIVVAVATEGDERVAALVDRV
                     LWVPEAPWMLSPVLTSIPLQMLAYHIATMRGLDVDQPRNLAKSVTVE"
     gene            7903..>9142
                     /locus_tag="P5516_07670"
     CDS             7903..>9142
                     /locus_tag="P5516_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92348.1"
                     /translation="MAPVKKPLIDPAPALALAERDKRNFRNVQIDAIGVGLANAASPF
                     LPVFLTYLNASSFQVGLLSSMPAFTGLLLAIPLGRFLQKQTNIVKWFSIARLLVIASY
                     ALTGIISFFLPEKVLVNSILLIWAVATIPQSLVSIAFSVVMNAVAGPDRRFELMSRRW
                     STMGLTTTLVVLAIGQLLDRIAFPLNYQLMFIGLSFGGLISYYYSSHIVLPSAAPPPM
                     ENSCDLRALYREYRDLVLREKPFVLFMVKRLLVMSGIQFSAPLIPLYLVRVAHVSDGW
                     ISIITMTQTAILVLGYFFWTKLSRKHGSRAVLLWTTFGISFYPLLISTTVVPWQIALF
                     AGIAGVFQAGLDLIFFDELMKTVPIEYSAIFVSFAQMMQFLAAIFSPMIGTLVADVFS
                     IPTGLIIASLIRFSGFIAFFP"
BASE COUNT         2094 a         2516 c         2012 g         2520 t
ORIGIN      
        1 tcatactcgt agatcaggca gcagcgcaat cgaccacaca tacccgtaat ttctgtcggc
       61 gtgagcgata ttccctgttc ttttgccatt ttgatcgaaa tggatgaaaa ctcagtaata
      121 aaacgggtgc agcagcgaga ttcaagaccg cacgcgccca tgcctcccat gatcttcgcc
      181 acgtcgcgtg ggccaatctg ccttaattcc acctgtgcgg gtgcgaactg acgctgcata
      241 tccgcgcgca gcgatttcaa ttcaactttt tcctcgtttt cactgctaaa gaggatgttc
      301 aagcgagttc catcaaagct atattcactc gcgattatct taataccgcg aagtttcaac
      361 tcttttgccc ggcttgcgca ttgttcaatt acttcgccct ctttattctg ccaaaattga
      421 cgttgaatca agtcctttgg tgtcgccagc ctgtcgattg gtttccaggt gctatcggcg
      481 ttttgacttt ctggtgttga gagggtgata atctcgccta attgccaccc acggctggtt
      541 tcaacgacta ccacatcccc tctttgaata ttttgaattt tcgtcgcatc aaaattatag
      601 atcttaccca cctttgaaaa ccgtacgcca acaattaact gagaagacgg tgtttccatc
      661 attcatttcc ttgtctaggt tctccagcgg gcgatcccgt aagtgccgcg atgctgatct
      721 gggctgccat cgcttccgcg attcgggtca aagattgtgc cgctgcagaa gtagggtcgc
      781 ttaacgcgat tggcttcccc gaatcccctc cgatccgcac cgccggttcg accgggatct
      841 cccctaaaaa tggaactccc agtcgttcag ccatttttct tccgccacca ttaccgaaca
      901 ggtccatttt tgttccatct ggcataggga gatagctcat gttctccaca atcccaagca
      961 caggcacgtt catctctgtg aacatttgta aaccccgttc cgcatcttca agtgaaacct
     1021 gttgcggcaa ggtcacgatc acgccgccac tcagcggaac gtgctgcatc aggcttaact
     1081 gggcatcgcc tgtgccgggg gggaggtcaa tgatcagata atccagctcg ccccagttca
     1141 cgtccgccag gaattgccgg atagcgctgt gcagcatcgg gcctctccag atcaacggtt
     1201 gacctttgcg taccataaag ccgatcgaca tcaactttac gccataagcc tttgctggaa
     1261 ctaacctgcc atcttcaggc gggggtagta aatccacacc catcatggta ggtatgttgg
     1321 gaccgtaaat atctgcatcc agtaaaccca cgctggcgcc tttttgtgcc aatatgacag
     1381 cgatattcac cgctaccgta gatttaccca ctccgccttt gcctgatgcc actgccacaa
     1441 catttttcac cggcaagctt aatacttcac ggtttttccc atcagatggc acacgggcgg
     1501 tcatgttgac ctttaccgag ctcacgcctg ggattgccag gacagcttct tttacctctt
     1561 tttcgatttt tttacttaat gggcaggctg gcgtggtaag cacgacagta agttccacac
     1621 tttctcccgc cacttggatg ttttctacca ttttaaggct taccaggtct ttatgcagtt
     1681 ccggttcaat cacgtttcgg agagcatctg ttaccatctc aactgtaagt ttcattgttc
     1741 ctcaattaat tcatattttg ccaggtgtcc aatcacttcc tcaagtgtat ccctataaag
     1801 ctgcaacagg tcatcatcgg aacctttaaa acgttttacg gtcaactggg cacaatgggt
     1861 gcagccgcgc atcgcgcgaa acgaatccgc attgcagccg gcgcagccac ctaacctgac
     1921 catcatcagg ctgaaggcac cctgcccgat ctcttcagcc tccgctgtgg tcaaacgatc
     1981 gatcaacgcc tgccattctt caccacggag attccgcaga gagggtatca cccggattgg
     2041 gaagagaatt tccgtatctg agttaagcat ggtcactatt gttacccggc ttgcgccttg
     2101 gctgcccgct ccgcttcttc ttgagcgtaa ttggtcggtc gaattttagc ataatattct
     2161 agcggttttc ctaagcgttc tttattgtag atatgagatt ccccgcgggt ataactggcg
     2221 atacgaaaat catggtccat ttttatcgcg tcaaatggac agaactcagc gcatgagccg
     2281 caattcatac acttgtcaat atcgatgtag aattccgctg attctggtac cggcttccct
     2341 gtttcttcat tcttcatccg cgtgatccag atgcactggg tcgggcatac tttcgcgcag
     2401 attccacacg cggtgcaacg gtatttcttt tgtccatctt cctcaattgt gtaaaccaga
     2461 aagggaatta ctctaaattc ctcaggaacc gttacagact cttctggata ctgtacagtg
     2521 aaaaagcctt cagccatcat cgtaccgcgt ttggcttttg ctcttcccaa cccaaattgg
     2581 tcagcgaaat aggttgaaaa gaagtgtcgt atggtaaccg ccagaccttt gattatccct
     2641 gtgccgttca tctgtgctcc tttttacctg tccacttcac ctaacacaat atcgacagac
     2701 cccagtatcg tcaccgcgtc tgcgatcttg ccaccaacac acattttttc cagaccggtc
     2761 aggttgatca gtgaaggcgc gcggacatgg tatctcttcg ggttgacctt cccatcagag
     2821 acaacataaa atcccagctc tcctctcggt ccttccactc gaccataaga ttcacccggc
     2881 gggaccttca ccaggtattg cacctttcca ttcatcaccg gcccttctgg gggtatctgc
     2941 tcgatgacct gcttgaggat gcgtatgctc tggcggattt catccagtct caccaaatag
     3001 cggtcgtaca catcaccgtg attacggtag caaacatcga actcgaatcg atcgtaaata
     3061 ctgtatgggt cggcccggcg aatatcatac ggcaccccag aagcgcgtaa gatggggcca
     3121 gccgcggaac aggcaatagc ctcttcagct gtcagcacac ccacaccttc acagcgcgaa
     3181 cggacaattt cattgtcggt caggtaacgg tcaaggtcat cgatcttcct tggcaggcgc
     3241 tcctcaacca ggtatttgac ttttgcaaag tcggccgcgc tgatatcgcg taccacacca
     3301 ccaaagcgga aatagttgca catcatgcgc gaacccgcca ccgattcaaa aatgtccagg
     3361 attaattcgc gctcttccag cgcgtacaaa gatggtgtaa agaatgctcc caggtcgtta
     3421 agcaaaaaac caatggacat ggtatgactg gctatcctgg taagttctgc catcataacc
     3481 cgcaagaatt cccctctttc agggggcggg agacccagca acttttcaac ggttatcgcg
     3541 tatccgaagt tattgctcat cgatgaaagg taatccagcc ggtcagtgaa aggcatattt
     3601 tgtagaaaag tgttgcgctc gccaattttc tcgtgatttc gatgcatata acccaacacg
     3661 ggttttaaag cgacgactgt ttcgccctcg aggacagcgc tcatccgaaa aaccccatgc
     3721 gtagatggat gctggggtcc gatgttcacc accatgcggt ctgtgtgaag tccatcctca
     3781 tcatttgcca ggttggcggt caaagcttta tagaggctct cgtcagcgtc ggtcgaaaca
     3841 ctctccgggt cgaactcctt tggatattgc acgttgtcat gaaagggatt cgtctgctct
     3901 ttgtgaaacg ctctcccttc tggccaacgg ctcttatagg gtttcacttc ctcctcaaaa
     3961 aggggttcgt gccagtcctt gcgcaacgga aagccggaga aaccttccca taataataaa
     4021 cgccgcaggt cataatggcc tgaaaaacga ataccaaaca tatcccaaat ctcgcgctcc
     4081 tggaaatcag cgccagggta cagctgcgtc acacttggta caacagggtt ttctcttgtg
     4141 gtaaatacct tgagttccag tccttccccc ccgatcgttt tatagaggtg gtaaatcacg
     4201 tcaaactgat catcaggata ctggtcaacc gcggtaacag aagacaggta atcaaaaccc
     4261 atttgttctt tcaggtaagt aaccacttca atcaaagaag caggcaaaac cacaatacct
     4321 gaaaagccag gtcttgtatc tggttccaca ttcggcgcga atcgctggag cagcgcttca
     4381 tactcagggt gaagaacagt caattcgctc atttgtcctc tcccgccgct tcattttctt
     4441 caacaactgt tggttcaggt cgatgcgtct ctgtataaat gaggtctttt ttccgtggat
     4501 cgataatatc aggacccaac acaggaacag ggatgtcttt ccccggttct ttcgtgtacc
     4561 agcctgattc tttcaattgc tcttttttta ctttctcctg taaggcgatc agcccgttga
     4621 gcaatgcctg aggggtggga gggcagcccg taatatacac gtccaccgga atataagtat
     4681 caatacctgg caccacgttg tagccttctt taaaaggtcc accgccagaa gcgcaggttc
     4741 ccatggcaat cacatacttg ggtaaagaca tctggttata caacctgatg atattaggca
     4801 gcattttttt ggtgaccgtt cccgccacga tcatcacgtc tgcctgccgc gggctggggc
     4861 gcatgacctc cataccaaac ctggagaagt catagcggct tgctgcggta caaatcattt
     4921 cgatcgcgca acaggccaat ccaaacatca tcggccatag tgagttgctg cgcccccagt
     4981 tatagatgcg ttcgagggtc gtgatggtta cctgctggcg caattcagca ggtatgtcta
     5041 tttcggtgtg attaagctca ggctttacat ccatttgttc ctcccggatg aaattatctc
     5101 cattgtaagc ggccgtacgg gattgtcaag tgtataatta gtataattat ttttatacta
     5161 tttcaaaatg aacactttca cgaaaattaa aaactacttg gaggcccatg catccgcgac
     5221 cgtttcacag ttgagccgtg acctcaacct caccaaagcg gacattcatt accacattcg
     5281 aatattgctt aaaaatggtg aaattgctgt acttccagac tcttaccagg ctggcgcagg
     5341 gcgaccagcg cgtcaatttg ttcgattgag gtctgccccc gacgcacttg cccgcttttt
     5401 gatcgaccat ttgatcaatc ttcatctcca aaacaaagat caacagtcaa gtcgagaggc
     5461 gctggctgaa tatctttcaa aatgcctgct ggaacaatat caacacttcg aggttcaaca
     5521 cgcatcccca gccgtcagac tggcgaagat agtcaagaac ctttcaactt ttggcattca
     5581 ccttcgttgg ttggcaggga aagacggccc gatggtatcc ctggaaaagg aagagctctc
     5641 cacgctgatc gatgatccag tgttggttga agcgatcgtg cgcgatctat tgctcagaat
     5701 tcaaaaaatg ttcatcaagt aaacacagcc gatcttttcc tggttcactc cacggtgacc
     5761 gattttgcca ggtttctcgg ttggtctaca tccaatccac gcatggtcgc aatgtggtat
     5821 gccagcattt gtaatgggat ggatgtgagc acagggctca gcatccaggg ggcttcaggc
     5881 acccataaaa cccggtcaac cagcgcggcc acacgttcat ctccttccgt ggcaaccgcc
     5941 acaacaatgc cacctcgcgc tttcgcctgt tcgatttgtg aaatcatctt ttcataccac
     6001 gggtctttga tggcaatggc caccactggc atattctcat cgatcaacgc gattgggcca
     6061 tgtttcatct caccagccgg ataagcctcg gcgtgaatat aagagatctc tttcaatttc
     6121 aacgcgcctt cataagcgat aggcatattg atcccccgtc ccaggtacag catatccttg
     6181 ctttcccgta aaaggtaagc ggtttcttca acttcacctt ctcggtcaag gcagcgtcct
     6241 gctagctcag gaattaatct caggtcctcc accaattttt ttcgctcttg aacggagaga
     6301 tcccccctta gatcagcgag taagattgcc agcatgtaaa ggtccaccag aggcgcagta
     6361 aacgctttgg tcgaggccac cccaatttcc ggaccggttt gcattgaaat cgatccatct
     6421 gctacgcgca tcgcctggga accgattgcg ttaacgatcg accaaatcgt ggctcctttc
     6481 ctgcgccctt cttccattgc agccaatgta tcggctgttt ctccagattg gctgatggca
     6541 atcagaatcg ttttgtcatt cacgattggg tcagagtagc ggaattctga tgctatctcc
     6601 aatgccgcgg gaataccagc gattctttca atcaagattc gcccaaccat cccggcatgc
     6661 gaggctgtcc cacaggcggt gagaacaatt ttctcaacct ctttcgcttt ttcaaccgtc
     6721 aggttcaatt gcggtagacg aattgtgcct tctttgaagt ttacccggcc agccagcgta
     6781 tctgtcagcg accgcacctg ctcgtggatc tccttttgca taaagtggcg atattcacct
     6841 ttttccgcag atacaggatc ccacgccact gaagatacct ggggatggat taccgtgcct
     6901 tcaatggtag aaatatgtac atcttcgcgg gtgactaccg ccatttgtcg tgattcaagg
     6961 aagatcaact tccgagtgtg ttcgagaatc gcgggtatat cagaggcgat aaagttttca
     7021 ccttctccca acccgatgac cactccgccc gcgttgccaa tgcgagccgc gataatcttg
     7081 tcaggttctc ttgaggtaaa aacgacaatg ccatgcgcgc ctttcagttg cccaagcgct
     7141 ttacgcgcag cgctttccaa atcaccggtc acgctcagaa acttttcaat caggtgaaca
     7201 atggtctccg tatcagtatc ggattgaaaa accacccctt ctgttaccag ctcttcctta
     7261 agttcgatga aattctctac aatgccgttg tgcaccacca ccacctctcc gctgccgctg
     7321 aggtgaggat gggcgttacg cgcgcttggt tcgccatgtg ttgcccagcg ggtgtgccca
     7381 attcccaacg taccagaaat gggttgctca cagacgttca tttccaggtt taccagtttt
     7441 ccggcattcc gccgcacttc aatttgtccg ttttttaaaa ctgccagtcc cgctgagtca
     7501 tacccccggt attccagctt cttcagccca cccaaaatga gcggagtggc gtccctttct
     7561 ccgatatagc ccacaattcc acacatggta tcctccaatt aaaatggtac ccgggtcttg
     7621 cgatccggaa ggaaaggata gaatggaatt ttcggtagac cagatctcat cgatctgaag
     7681 ggcatccgct gatctttcga tttttccggg catcccggtt agctttacct cgcggtaaag
     7741 aaccctctcc tcgtcaacct gtcagattga caggtccatg cgctattctt ccgtgattgg
     7801 ttccgtttcc tttcacgtgc attatacctt aatcgaaatc cttatcttct ttgaggtctc
     7861 ttgctcgttg gtataatcga ctcattctgt ttaccagaaa tcatggcacc tgtgaaaaaa
     7921 ccactaatag atcccgctcc tgctcttgca ctcgccgaac gcgataaacg gaattttcgc
     7981 aatgttcaga ttgatgccat tggtgtcggc ctggccaacg cggccagtcc tttcttgcct
     8041 gtcttcctca cttatttaaa cgcttcatct ttccaggtcg ggttgctgtc ctccatgccg
     8101 gcattcaccg ggttgttatt agccattccc ctgggcaggt ttcttcaaaa gcaaacgaat
     8161 atcgttaaat ggtttagcat tgccagactt ttggtcatcg ccagttacgc gcttaccggc
     8221 atcatttcgt tctttctgcc cgaaaaagtg ctggtgaact cgatcttgct catctgggcg
     8281 gttgccacaa tcccccaatc actggtatct attgcgtttt ctgttgttat gaacgctgtg
     8341 gccggaccag acagacgttt tgaattgatg tcccgccgct ggtcaaccat gggtttgacc
     8401 acaaccctcg ttgtgctcgc catcggtcag cttctcgaca ggatcgcctt cccattaaat
     8461 taccagttaa tgttcatcgg cttgtccttc ggagggttga tcagttatta ttactcctct
     8521 cacatcgtct taccttcagc cgctcctccc ccaatggaga acagctgcga ccttcgcgcc
     8581 ctataccgcg aatatcggga cctggtttta cgtgaaaagc cctttgtttt gttcatggtc
     8641 aagagactac tggtaatgag cggcatccag ttttccgcgc ctttaattcc tctgtacctg
     8701 gtgcgtgtgg ctcatgtatc ggatggatgg atctctatca tcactatgac ccaaaccgca
     8761 attttggtcc tgggttattt tttttggacc aagttgtcca ggaaacatgg ttcgcgcgcg
     8821 gttcttttat ggacaacctt tggcatctcg ttctatccac ttctcatctc caccactgtt
     8881 gtgccctggc agatcgcctt gtttgccggt atcgccggtg tttttcaggc tgggttggac
     8941 ctgatttttt ttgatgaact catgaaaaca gttcccattg aatacagcgc catcttcgtt
     9001 tccttcgctc aaatgatgca gttcctggca gcgatttttt cccctatgat tgggacgctt
     9061 gtcgcggatg tattctcgat tcccacaggt ttaatcatcg cttcgcttat ccgtttttca
     9121 ggttttattg ctttttttcc gc
//
LOCUS       DAOTLW010000054         8963 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_7902_length_8963_cov_9.519308, whole genome shotgun sequence.
ACCESSION   DAOTLW010000054 DAOTLW010000000
VERSION     DAOTLW010000054.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8963)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8963
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_7902_length_8963_cov_9.519308"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            171..692
                     /locus_tag="P5516_07675"
     CDS             171..692
                     /locus_tag="P5516_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92349.1"
                     /translation="MMGDLLERVMAQRDALKKLIAKLPGFKGYYERADRRSADKLLRE
                     TISDKFQAQWARLSSIQRELIAGGHIEVMDEIETGAIRLRQFIDRVKTAAYGYSGLFD
                     ATKINEAELDKVYQYDLTLLELADEIASAIDNLEASIGSDGLPAAIRNVNQKAQDCLD
                     AFNQRSELFKFGG"
     gene            738..1790
                     /locus_tag="P5516_07680"
     CDS             738..1790
                     /locus_tag="P5516_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024813.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRT92350.1"
                     /translation="MARIFDVVEYPSEMTDELVHRFPETGVADLRIGSQIIVRESQRV
                     VFMRDGHALDVFGPGRHTIVTANIPLLINLVGKAFNDRTPFTAEVYFVSMREFVDRKW
                     GTPQPILVRNAGVGLGIALLQGFGTYSFQVADAQQFVTQVVGAQGSYRMTDIENRLRT
                     MLLSKIQDLLGETGSKKNVLEMIGLTEELGTGVRVKAQDDFAALGLMLKSFYIGSLKP
                     SDKSAEELRAMGMLDMATYAQLQASDAMREAARNEGGGAGLTAGIGAGLGIGNVLSQS
                     LQGIGTTQAAGAAGAAQGGTAAIPSVMTPAEAANVLKVAEADVVAAITAGDLKAKKIG
                     ESYRISKEALEEFLKG"
     gene            1889..3031
                     /gene="sucC"
                     /locus_tag="P5516_07685"
     CDS             1889..3031
                     /gene="sucC"
                     /locus_tag="P5516_07685"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HRT92351.1"
                     /translation="MKLHEYQAKNLYAKYNIPIPKGRIATSASEARRLCEELGGKVVV
                     KAQVLVSGRGKAGGVRLVKTPEEAEEVTTSILSMKIKDLPVRKVLVDEAISISKEIYL
                     GITNDRAAQKPVLIACGAGGVDIEEVAQKTPGKIAKMEVDSLLGLRDYQIRDIALSID
                     LPRQFWRPFGEIIYALWKVYTDCDATMAEINPLVITADNHLVALDAKIVLDENALFRH
                     PDLADLRDIDGEDWAETQGRKFGVSFVKMEGDIGCMVNGAGLAMATMDIISHFGGKPA
                     NFLDIGGGASSEKVASAIRLILADRNVKAILINVFGGITRCDEVAEGIVRACRDVASD
                     VPMVVRLVGTNEDKARQILSGASLVYADSLVEGAKKAIDMVRKEDK"
     gene            3028..3888
                     /gene="sucD"
                     /locus_tag="P5516_07690"
     CDS             3028..3888
                     /gene="sucD"
                     /locus_tag="P5516_07690"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HRT92352.1"
                     /translation="MSILATNKVRLLVQGITGNEGLFHTQQMMAYNTNVVAGVSPGKG
                     GDWVLDGKVPVFDTVKNAVEVTGANASVIFVPARYTADAIFEAVDGGMELVVCITEGV
                     PVLDMMRVKEYLKGKTTRLIGPNCPGILTPGEVKIGIIPGNISLPGNIGVISRSGTLT
                     YEVLYSLYKEKRGVSTCVGIGGDPVKGMNFVDVLELFEADAKTDAVVMIGEIGGNDEE
                     MAAEFISRQMTKPVAAFIAGISAPPGKRMGHAGAIVEGRSGSAESKIEKLKSNGVKLA
                     DYPEQIATLI"
     gene            complement(3935..4171)
                     /gene="acpP"
                     /locus_tag="P5516_07695"
     CDS             complement(3935..4171)
                     /gene="acpP"
                     /locus_tag="P5516_07695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HRT92353.1"
                     /translation="MSDTFEKVSEVIVEVLKVDASEVKEDSRFVEDLKADSMDQFFLI
                     DGFCEKFDLNINDEDARSIKTVADAVKYIDTHKK"
     gene            4286..5311
                     /gene="fni"
                     /locus_tag="P5516_07700"
     CDS             4286..5311
                     /gene="fni"
                     /locus_tag="P5516_07700"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HRT92354.1"
                     /translation="MNKTEVRKDQHITINLEQNVNSALTTGLEDYVFTHQALPEIDLQ
                     QVNTETTFLEKNLKIPLLISSMTGGTDRGDRINCALAEAAQVSGIAMGIGSQRLQLEK
                     GEKYQKGLLRKLAPGIPLYANIGAIQLNNGYGVDECQRAVDMIEADGLILHLNPLQEA
                     LQPEGQTNFSGLLRKIEEVCRVIKVPVIVKEVGWGINLETAKKLVQCGASAIDVAGAG
                     GTSWSEVEKHRSVEDQHYRIASKFRDWGIPTAECINEIRQGLSKVPLIASGGLRGGLD
                     LAKAIALGANIGGIAGPFLSAANRSVESVLTLIQEIELELRITMFAAGVVDIRSLANT
                     PLVRKSK"
     gene            5315..6331
                     /locus_tag="P5516_07705"
     CDS             5315..6331
                     /locus_tag="P5516_07705"
                     /inference="COORDINATES: protein motif:HMM:NF012568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HRT92355.1"
                     /translation="MVETQLLKDCWRALDEELDCGIARFTERCEPAFGKMIRYQLGWN
                     GNGFDPARTGKRIRPLLLLFVTHALEGEWRKALPAAAAVELVHNFSLVHDDIQDKSET
                     RRGSDTVWVKWGIAQAINTGDAMLTLANLEMALLENSQEPSIVMAAVRSLNKATFDLT
                     VGQYLDLAHEKAEDVTMADYFEMVKGKTGALFAVCFRLGALIAGKKPTELIKYSDLGE
                     KFGLAFQIQDDYLGIWGQHGFTGKTVTGDLLNRKKTFPVHYALENLPKARDYWVKHET
                     FTVKDLEWFKNCLENGAVDKITLAEIGRIYADSTAQLSFLFQGSKSASELFQLLNSLF
                     ERNR"
     gene            complement(6336..6794)
                     /gene="folK"
                     /locus_tag="P5516_07710"
     CDS             complement(6336..6794)
                     /gene="folK"
                     /locus_tag="P5516_07710"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:NF013456.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HRT92356.1"
                     /translation="MSKFYILLGSNIEPEKNIPLALGFLGTSPEWVLLSKSGVWRTKP
                     IGSCCTDFLNVAVLVETPLSPNELKNRLAAIESAMGRVRTEDKNAPRVIDLDIVAEDD
                     RVIDKEISVFDHILLPLSEIAPNLLLPGSLITLAEASAARKKFTAAVRVD"
     gene            complement(6801..7154)
                     /locus_tag="P5516_07715"
     CDS             complement(6801..7154)
                     /locus_tag="P5516_07715"
                     /EC_number="4.1.2.25"
                     /EC_number="5.1.99.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA];
                     GO_process: GO:0046656 - folic acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HRT92357.1"
                     /translation="MDEIFIQDLLIRSIIGVTEKEREQPQDILVNIILFTNTAQAGLS
                     DSVDDSVNYRTVTKKVLAHTEAIKRFTVEALAEDIAQLCLLEKGVQSVKVRVEKPGAV
                     RFAKSVGVTITRKKE"
     gene            complement(7164..7889)
                     /locus_tag="P5516_07720"
     CDS             complement(7164..7889)
                     /locus_tag="P5516_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024950.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HRT92358.1"
                     /translation="MTLTDKTILITGAARRLGKALAIACAKQGADIVLHYNSSQSEAE
                     EVRDQVHLLGRDCLILKADLADPQQVERMMSTAVAKTNLYGLVNNASIFKSMGFMDTT
                     LPDWNEHIAVNLTTPFLLTQHFARHYTLAETGRVLNLVDWRALRPGRDHFPYTISKSG
                     LVALTKSTALQLAPRINVNAIALGAILPPVGQPEDDRLIKNVPQKRWAELDEFTRTAL
                     FLLDGPDYITGEVIHLDGGRHLV"
     gene            7995..8198
                     /locus_tag="P5516_07725"
     CDS             7995..8198
                     /locus_tag="P5516_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-associated domain-containing
                     protein"
                     /protein_id="HRT92359.1"
                     /translation="MDTISLKIPNISCGHCIHTIKMELMEMEGVTEVTADLAAKQVNV
                     VYSPPANEEMIKNLLKEINYPAE"
     gene            8221..>8963
                     /locus_tag="P5516_07730"
     CDS             8221..>8963
                     /locus_tag="P5516_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR00003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper ion binding protein"
                     /protein_id="HRT92360.1"
                     /translation="MAGKKQIILPITGMTCANCVATIERNVKKLDGVEDTMVNLVTEK
                     ATVTYDSDKLDLNQISDRVIRAGYGVVTSEVVLLVQGVNDSGAAASLETQIKKIEGVR
                     SVSVNLSSSRVLISFISTIISRDELLKGIKGLGISATVLGDEQGEDEVTARQRDADLQ
                     RRLLIIGILFTLPVFILSMARDFHLLPAIIVDSPWLNYLLLLLATPVQFYVGWQYYQG
                     AYRSLRGGSANMDVLIALGSSVAYFYSIP"
BASE COUNT         2352 a         1980 c         2447 g         2184 t
ORIGIN      
        1 gcgccaaaca atggaagtta gcttgtcttc agtgcattct tgtgtatcat cttgtctttt
       61 tcttttgctc aattagccca atctaacact tttctcataa atatccgtat aatatatggt
      121 atatctacct gcgatatggt tatttaccaa tcatcagaac acggaggaat atgatgggtg
      181 atttattgga aagagtgatg gcgcaacggg acgcgttaaa aaagttgatc gccaagctgc
      241 cgggctttaa gggctactat gaaagagcgg accgacgctc tgcagataag ttgttgcggg
      301 agaccatttc agacaagttt caggcgcagt gggcgcggct ttcaagcatt caaagggaac
      361 tcatcgctgg tgggcacatt gaggtgatgg atgaaatcga aactggcgcc atccgcttac
      421 gccagttcat cgaccgggtc aagacagcgg cctacgggta ttcaggcttg tttgacgcga
      481 cgaaaattaa cgaagcagag ctggataagg tctatcaata tgacctgacg cttttggagc
      541 tggcagatga gattgccagc gctattgata atcttgaagc atcgattggc agtgatgggt
      601 taccagccgc gatccgcaat gtcaatcaaa aggctcagga ctgcctggac gcgttcaatc
      661 agcgctcgga gctgttcaaa tttggcgggt aaaaaacacc cggttgtatc caattatttt
      721 gataagggag taatcacatg gcaaggattt ttgatgtagt tgaataccca agcgagatga
      781 ccgatgaact ggtgcaccgc ttccctgaaa ccggcgtggc tgacctgcgc ataggttctc
      841 agatcatcgt gcgcgaatca cagcgcgtgg tcttcatgcg cgatgggcac gcgctggacg
      901 tttttggtcc ggggcggcat acgattgtca ccgcgaacat cccgctattg atcaacctgg
      961 tgggcaaagc attcaatgac cgcacgcctt ttaccgctga agtctacttt gtttcaatgc
     1021 gcgagtttgt cgaccgcaag tgggggactc cgcagcctat ccttgtgcgc aacgcagggg
     1081 tggggctggg tatcgctctg ctgcagggct ttggcactta cagcttccag gtggcagatg
     1141 cgcagcagtt cgtcacccag gtggtaggcg cgcaggggtc ttatcgcatg acagatattg
     1201 aaaaccgctt acgcaccatg ctcctttcca agatccagga cctgctggga gagaccggca
     1261 gcaagaagaa cgtgcttgag atgatcggtc tgaccgagga gcttggcaca ggcgtacggg
     1321 tgaaggcgca ggatgacttc gcggcgctgg gcttaatgct gaagagcttt tatattggca
     1381 gccttaaacc gtctgacaag tcggcggaag agctgcgcgc catgggtatg ctggatatgg
     1441 ccacctacgc tcagctgcag gcatcagacg ccatgcgcga agccgcgcgc aacgagggtg
     1501 gcggagctgg gctgaccgcg ggtattggcg cggggctggg catcggcaac gtcctatcac
     1561 agtcactgca gggaattggc accacccagg cggccggagc tgctggcgca gcgcaaggcg
     1621 gaaccgcagc gatcccgtcg gtgatgacac cggcagaagc agccaatgtt ctcaaagtgg
     1681 cagaggctga cgtggtggca gccatcactg ccggcgacct aaaagccaag aaaattggtg
     1741 aatcataccg catcagcaaa gaagcgctgg aagaattcct taaggggtaa aaccagacga
     1801 aggaaagaca ggcccgggat caactctcgg gcttttttat tccgtgtata attctatggg
     1861 ttaaatcacg aatgatgcag gtggtgagat gaaacttcac gaataccagg cgaaaaacct
     1921 gtacgccaaa tacaacatcc ccataccgaa aggcaggatc gcgacctccg ccagcgaagc
     1981 caggcgtttg tgcgaagaac tgggcggaaa ggtggtcgtg aaagcgcagg tgctggtgag
     2041 tgggcgtgga aaagcggggg gagtgcggct ggttaaaacc cctgaagaag cagaagaagt
     2101 gacgaccagt atcctatcca tgaaaattaa ggacttgccg gtacggaagg tcctggtaga
     2161 tgaagccata tccatcagca aggagattta tctgggcatt accaatgatc gcgcagccca
     2221 gaaaccggtg cttattgcct gcggcgcagg tggagtggat attgaggaag tcgcacaaaa
     2281 aacaccaggt aagatcgcca aaatggaagt tgactcttta ttggggctga gagattacca
     2341 gatacgagat atcgccttga gtatcgacct gccgcgccag ttttggcgtc ctttcggtga
     2401 aattatctac gccttatgga aggtttacac agattgtgat gcgacgatgg ctgagatcaa
     2461 tccgctggtg attacagcgg ataatcacct ggtagcgctg gatgccaaga tcgtgctgga
     2521 tgaaaatgcc ctattccgcc atcctgatct ggcagacttg agagacatag acggtgagga
     2581 ttgggctgaa acacagggga gaaaattcgg tgtttctttc gtcaaaatgg aaggcgacat
     2641 tggctgcatg gtgaatggcg cggggttggc gatggccacg atggacatta tctcccattt
     2701 tggtggaaaa cccgccaact tcttagacat tggcggaggg gcatcttcgg aaaaagtagc
     2761 ctcggctatc cgcttaatat tggcggacag gaacgttaaa gccattttga tcaatgtctt
     2821 tggcggcatc actcgctgcg acgaggtcgc ggaggggatt gtaagagcct gcagagacgt
     2881 cgcttctgat gtgcccatgg tggttcggct ggttggcacg aatgaagaca aagcccgcca
     2941 aatcctttct ggtgcttctc tggtctatgc cgattcgctt gtagagggcg cgaaaaaagc
     3001 gatcgacatg gtacgcaagg aggacaaatg agcatacttg ccacgaacaa ggtacgcctg
     3061 ctggtgcagg ggataactgg caatgagggc ctctttcaca ctcaacagat gatggcttac
     3121 aacacaaatg tggttgccgg agtcagcccg ggaaaaggcg gagactgggt gctggatggg
     3181 aaagtaccag tttttgacac cgtgaagaac gcagttgaag ttaccggcgc gaatgcttca
     3241 gtgatatttg tgcccgcgcg atatacagcg gacgccattt tcgaggcggt tgatggcggg
     3301 atggaactgg tggtgtgcat cactgagggc gtgccagtgc tggacatgat gcgcgtcaaa
     3361 gaatatctca aaggaaagac aacccggttg attggtccga actgcccggg aatcctaacg
     3421 ccgggcgaag tgaagatagg tatcatccct ggaaatatct cgctgcctgg caacatcgga
     3481 gtgatctccc gctctggcac cctgacttat gaagtgctct attccctcta caaagaaaaa
     3541 agaggggttt caacctgtgt gggcatcggt ggcgacccgg ttaaaggaat gaattttgta
     3601 gacgtcctgg aattgttcga agcggacgcg aaaaccgatg cggtggtgat gattggtgag
     3661 attggcggga atgatgaaga aatggcagcg gagttcatca gcaggcagat gacaaaacct
     3721 gtcgcggctt ttattgccgg tatttcagca ccgcctggaa aacgcatggg gcatgcgggc
     3781 gctattgttg aagggagaag tggcagcgct gaatcaaaaa ttgaaaaact gaaatcaaat
     3841 ggcgtgaaac tggcagatta tcctgaacag attgcgactt taatataaaa aacaggaaga
     3901 gttgtggtta actcttcctg tttgttgacg aagactattt cttatgggtg tcaatatact
     3961 tcacagcatc agccacggtc ttgatggacc tggcgtcttc atcattgata ttgagatcaa
     4021 atttttcaca gaatccgtca atcaaaaaga actgatccat tgaatccgct ttcaggtctt
     4081 ccacaaagcg agagtcttct tttacctctg aagcgtccac ctttaagact tcaacaatta
     4141 cttcacttac tttttcaaac gtgtcactca tctcctaagc ctcctggacg gattattgcc
     4201 atttttacta aattgtaact gacctcaact ttctgtcaag tacacaaatc agcatttttt
     4261 aaagggcttt ggtatactca agaagatgaa taagaccgaa gttcgcaaag atcaacatat
     4321 taccatcaat ttggaacaga acgtcaactc cgcgctgaca accggactgg aagattacgt
     4381 ttttactcat caggcgctgc cagaaataga tctgcagcag gtcaacacag agaccacgtt
     4441 tctggaaaaa aaccttaaga ttcctttatt gatctcttca atgactggtg gcacagatag
     4501 gggggaccgc atcaattgcg cacttgccga agcagcgcaa gtatcaggta ttgcgatggg
     4561 aattgggtcg caacggttac aactggaaaa aggcgaaaaa tatcaaaaag gcttgttgag
     4621 aaaattggca ccaggcattc cactttacgc taatatcgga gccattcaat taaataatgg
     4681 ctatggcgtt gatgaatgcc agcgcgcggt ggatatgatt gaagcggatg gcctgatttt
     4741 gcatttaaat ccgctgcagg aagcgctgca accagaaggg caaaccaact tctcaggctt
     4801 gttgcgtaag attgaggaag tttgccgagt gataaaggta ccagtgatcg tcaaagaagt
     4861 ggggtgggga atcaacctgg aaacggcgaa aaagcttgtt cagtgcgggg cgagcgccat
     4921 cgatgtagcg ggggcgggcg gcacctcatg gtctgaagtg gagaaacacc gcagcgtgga
     4981 agatcaacat taccgtatcg cttcgaaatt tcgagattgg ggcataccca ctgcagaatg
     5041 catcaacgaa atccgccagg gcttgtccaa agttcctttg atcgcatccg ggggtttacg
     5101 tgggggattg gacctggcga aagcgattgc cctgggggcg aatattggtg ggattgccgg
     5161 tccattcttg tccgcagcca atcgttcggt tgagtctgtt ctgacgctta ttcaagagat
     5221 tgagctggaa ttacgtatca ccatgtttgc tgctggtgta gtggatattc gatcactggc
     5281 aaatactcct cttgttcgta aatcaaaata aatcatggtc gaaacccaat tgttgaaaga
     5341 ctgctggcgt gctttggatg aagaactgga ttgcggcatc gccagattca ctgagcgatg
     5401 cgagccagcg ttcggaaaaa tgatacgcta tcagctcggg tggaacggta atgggtttga
     5461 tcccgcgcgc accggcaaac gcatcagacc tctattattg ctgttcgtaa cgcacgcgtt
     5521 ggagggtgaa tggcgaaagg ctttgcccgc cgccgcagcg gttgagctgg tgcacaattt
     5581 ttcacttgtt cacgatgaca tccaggataa aagcgaaaca agacggggaa gcgacacggt
     5641 atgggtcaaa tggggaatcg cgcaagccat taatactggg gatgccatgc ttacgctggc
     5701 aaacctggaa atggcgctct tagagaattc tcaagagccg tccattgtaa tggccgctgt
     5761 gcgctctttg aataaggcga cattcgacct cacagtgggg cagtatctcg acctggcgca
     5821 tgaaaaggcc gaagacgtca ccatggcaga ttattttgaa atggtaaaag gcaaaactgg
     5881 tgctttgttt gcagtttgtt ttagactggg cgcgctgata gcggggaaaa aaccaaccga
     5941 actgattaaa tattccgact tgggtgagaa gtttgggctg gcttttcaaa ttcaggacga
     6001 ttaccttgga atttggggtc agcatgggtt caccgggaaa acagtgacag gcgacttgtt
     6061 aaacaggaaa aagacctttc ctgtgcatta tgcgctggag aacctgccta aagcccgcga
     6121 ttactgggta aaacatgaaa catttacagt aaaagacctg gaatggttta aaaattgtct
     6181 tgaaaatggc gctgtggata aaattacttt ggctgagatt gggcggattt acgctgacag
     6241 cactgcacag ctgtctttct tatttcaggg ttcaaagagc gccagtgaac tctttcaatt
     6301 acttaacagc ctttttgaac ggaacaggta gcgctttaat ccacgcgaac agcggcagta
     6361 aacttttttc ttgccgccga tgcttctgcc agtgtgatta aagaacctgg caacagcaaa
     6421 ttcggcgcga tctctgagag ggggagtaag atgtggtcaa acaccgaaat ttctttgtcg
     6481 ataaccctgt catcttctgc aacgatatct agatcgatca cccgcggagc gtttttatct
     6541 tctgttcgca cccgacccat tgcagattca attgcggcaa gccgattttt taattcgttg
     6601 ggagaaagag gtgtttcgac gagaacggca acatttagaa agtccgtgca acaactgcct
     6661 atcggttttg tgcgccatac accggacttg cttaggagaa cccattctgg agaggttcca
     6721 aggaaaccca acgcgagggg gatatttttt tcgggttcaa tatttgaccc caataagatg
     6781 taaaacttgc tcatggggat tcactctttt ttccgtgtta tggtcactcc cactgatttt
     6841 gcaaagcgca ctgcaccggg tttttcgaca cggactttca cgctttgcac acctttttca
     6901 agcaggcaaa gctgggcgat atcctcagcc agggcttcaa ctgtgaagcg tttaatggct
     6961 tcggtatgtg ccagcacttt tttggttaca gtgcgataat taacgctgtc gtccacgcta
     7021 tctgaaagac ccgcttgagc cgtatttgtg aaaaggatga tattcaccag aatgtcttga
     7081 ggttgttcgc gttccttttc ggtaacgccg atgatactgc gaatcagcag gtcttgaata
     7141 aaaatttcat ccattctgac tccttaaaca aggtgacgtc cgccatccag atgaatgact
     7201 tcgccggtga tgtaatcagg tccgtcgagc aagaacaggg cggtgcgggt gaactcgtcc
     7261 aattcagccc atcttttctg agggacgttc tttatcaagc gatcgtcttc tggctggcca
     7321 actgggggca gaatggcgcc cagagcgata gcgttcacat tgatgcgtgg ggccaactgc
     7381 agcgcggtgg atttcgtcag cgcgaccaac ccggatttgc tgatggtata tggaaaatgg
     7441 tcacgacctg gccttaatgc gcgccagtca accaggttta aaacgcggcc ggtttcggcg
     7501 agcgtgtaat gacgggcgaa gtgttgtgtc agcaagaatg gcgtagttaa attcacagca
     7561 atatgttcat tccagtcggg taaagtggtg tccataaatc ccatggattt gaaaatcgag
     7621 gcattattca ccaacccgta aagattcgtc tttgcaacgg ctgtcgacat catcctctca
     7681 acctgttgtg gatcagctag atccgccttg agaatgaggc agtcgcgccc caacaaatgg
     7741 acctggtcgc ggacttcttc agcctcggat tgggaagagt tgtaatgcag cacgatatcc
     7801 gcgccctgtt tggcgcaggc aatggctaag gcttttccca gacgacgggc agcgccagtg
     7861 atgagaatag tcttatccgt taatgtcatc gtatgtatat cctatgagga aaaagagcga
     7921 ttgtcaagtg aaaaaaaccg gttgtaagtg gacaataata gtcctaaatg gtagaattaa
     7981 tcaaaaggag aaaaatggat acaatctcat taaaaatccc caatattagt tgtggtcatt
     8041 gtattcatac catcaagatg gaattgatgg agatggaagg agtgaccgag gtcacggccg
     8101 acctggcagc gaaacaggtg aatgtggttt acagccctcc ggcgaacgaa gagatgatta
     8161 aaaatctctt aaaagagatt aattacccag cagaataaac ggaaaagaaa aagtctggca
     8221 atggcaggaa agaaacagat aatcttacct attactggca tgacctgtgc caattgcgtt
     8281 gcgaccattg aacgcaacgt aaagaaactg gatggtgttg aagacaccat ggtcaacctg
     8341 gtcactgaaa aagcgactgt gacttatgat tccgataagc ttgacctcaa ccaaatttct
     8401 gatcgggtta ttagagccgg atatggggtt gtaacgagcg aagtcgtatt gctggtacag
     8461 ggggtcaacg atagcggagc cgcggcgtct ttggaaacac agattaaaaa gatcgaaggg
     8521 gtaaggtcgg tttcggttaa tctctcttcc tccagagtgt tgatttcgtt catctcaacc
     8581 attatttcac gtgacgagtt attgaaaggc ataaaaggtt taggtatatc agccacagta
     8641 ttgggtgacg aacagggaga ggatgaagta actgccaggc agcgcgacgc tgaccttcag
     8701 cgacgtttgt tgatcatcgg gattttattt acgctgccgg ttttcattct ttcaatggca
     8761 agagacttcc acctattgcc agcgattatt gtagattccc catggttgaa ttatctgttg
     8821 ctactcttag ccactccggt gcagttctat gttggctggc aatactacca gggcgcttac
     8881 aggtcgctgc gtggtggttc ggctaatatg gacgtgctga tcgcgctggg ttcctctgta
     8941 gcgtattttt attcgattcc aat
//
LOCUS       DAOTLW010000055         8430 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_8486_length_8430_cov_9.791642, whole genome shotgun sequence.
ACCESSION   DAOTLW010000055 DAOTLW010000000
VERSION     DAOTLW010000055.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8430)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8430)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8430
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8486_length_8430_cov_9.791642"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="P5516_07735"
     CDS             complement(<1..845)
                     /locus_tag="P5516_07735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT92361.1"
                     /translation="MLQAPSNTEIRQAVSQDITAITDFVDKASLTHRHLDWRPLVEWV
                     SQPPFLLRFANQQLTALLSCAPDPEGVAWIHAFIMDHWSNQVNAVWHSLFEPALIELA
                     VTRCQVFSLSLSDWYTRLLRSSGFTPFENIVVLHWGREVPPVKNMPPEVLIRPMEPAD
                     LNQVAEVDRQTFEAAWVISRASLERAYLQSAHASVAEVNEQIIGYELSTASSLSSHLA
                     RLAVLPEFRHLNIGYSLVAQLLEYFTQRGIWQITVNTQDNNQASLSLYKKLGFFQTSE
                     SFPVFT"
     gene            complement(846..1382)
                     /locus_tag="P5516_07740"
     CDS             complement(846..1382)
                     /locus_tag="P5516_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92362.1"
                     /translation="MTAEIEKLKQQAIKDYNRRKYDAAIAGFQSCLEFYEKQNDELLV
                     AEMRNNISVALLGKNAAQEAYDIVKGTDETFQKVGDLKGQGMALANTAAALEALDKKE
                     EALALYEHTLDIFKEAGEKELRINILRRVSDLQLKTKRGFQALASMEAAYNQREKPTI
                     KDSVFKTTLAAIRNKLFK"
     gene            complement(1386..2063)
                     /locus_tag="P5516_07745"
     CDS             complement(1386..2063)
                     /locus_tag="P5516_07745"
                     /inference="COORDINATES: protein motif:HMM:NF024949.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HRT92363.1"
                     /translation="MEVSPLEEQSQLISARLLAAREVSEKTLARVAEQLGVSVKMVKN
                     YESGQSTPSLPQLEVLSELYRIPIENLLSGNPDLLKKTILPREKVNNFLEIRNRVIAA
                     TLKQQRLAQNWTIKKLAAAVGISAAILPRYESGETAIPAPILEHLCSQLGIPLKSLYS
                     SITLQNSEPPPIASPSQDSSQLPEEVQSFIQNPANLPYLELAKRLSGMDAAKLRAIAE
                     NLLEITF"
     gene            complement(2126..2833)
                     /locus_tag="P5516_07750"
     CDS             complement(2126..2833)
                     /locus_tag="P5516_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HRT92364.1"
                     /translation="MNARILIVDDEPLYLRLIKVNLEQEGYLIATAKNGQEALDLVSH
                     EMPDLIILDVMMPKLDGITTATRIRQFSNVPIIMLTALGEERDRVRGLNLGADDYVVK
                     PFSATELVARVKAVLRRSQLKENAGRSRILTHGDLKIDIAKAEVFKGDHPVYLSATEY
                     KLLIHFAHNLGKVLSAEELLTTTWGPGYKDEKEILWVSIARLRQKLEDDPHEPVHIVT
                     RTGMGYQMPALDEQAKK"
     gene            complement(2843..4057)
                     /locus_tag="P5516_07755"
     CDS             complement(2843..4057)
                     /locus_tag="P5516_07755"
                     /inference="COORDINATES: protein motif:HMM:NF014567.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HRT92365.1"
                     /translation="MSNEFLLDEIDKEKLLQFCHFVSSSDDWKATLDKMVTYIREFFI
                     FDNLVVYLGDSTIKRMDVLYARAMGRGRSKEADVSWGEDIASRVVAEKKAFIIQPENK
                     TASDRLQSPFLLGVPLSISKEITGVLIFIRFGGPDFEPGSKEFAELMGTLFASVIRQK
                     TINDYEKELSGLRRSSNLQDDFISTITHELRNPLGFIRGYTTTLLREDADWDEKTKHD
                     FLEIIDRETGHLGELIDDLLDSSRLQSGNMRFDFQSLHIDAIIRDQVKRAEYTNPSQV
                     IKLELEQQLPTVVGDPRRLAQVIDNLIENSKKYAPGSVITIKVFQKEANLVIEFSDTG
                     PGIPEKYLPNMFSRFFRVPELEKSVYGTGLGLSICKQIINNHNGEISVSSSQGEGIKF
                     TITLPLNHDVSL"
     gene            complement(4415..5263)
                     /locus_tag="P5516_07760"
     CDS             complement(4415..5263)
                     /locus_tag="P5516_07760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HRT92366.1"
                     /translation="MAQKTGLGKGLDALIPAGLNTTPTRADSYAPVSMIIPNPQQPRD
                     TISPESLAELSSSIREHGVLQPLVVSHDPVTGMYTLIAGERRLRAAKIAGLEKVPIII
                     RTVSEQQRLTQALIENIQRADLSPLETARGFRRLIDEFNLTHETLADQVGKSRVTVTN
                     TLRLLNLPAEAQEALAASRISEGHARALLGLPTPQAQLSALHTVLSQQLNVRQTEELV
                     RKLTGIKPRSLPPVEVDPHFQELEERLRVKLGTRVTLRSGKKGGTITIHYYSDEELNG
                     LIEKLT"
     gene            complement(5264..6064)
                     /locus_tag="P5516_07765"
     CDS             complement(5264..6064)
                     /locus_tag="P5516_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT92367.1"
                     /translation="MTRVLTLVNQKGGVGKTTTAINLSAYLGLFSKRVLLIDLDPQAN
                     ATSSLGINKYEVKNGTYEVLIGEQPILPNILLNPRLKIALLPSSPALAGSEVELIGME
                     GREFRMREALKPALDRYDYIIIDCPPSLSLLTVNGLIAARDGVLIPVQCEYLALEGIG
                     QLTQTLNLVRSSLFPEMRIRGVILTMFDIRTNLSTDVVNEVRKFFPNLVYDTVIPRSV
                     RLAEAPSYGLPISHYAPSSSAASAYAALTREILAQDGEPSPPFKEQVA"
     gene            complement(6061..6924)
                     /locus_tag="P5516_07770"
     CDS             complement(6061..6924)
                     /locus_tag="P5516_07770"
                     /inference="COORDINATES: protein motif:HMM:NF012515.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HRT92368.1"
                     /translation="MPDLATIEPVDYLIIGHITQDVTPAGLSLGGTASYAALTARAFG
                     LRVGIVTSCSPDLQPPELNGIAIARKPSPFTSTFRNTYTPTGRIQHILHVADLLSLED
                     VPPAWRSAPIVHLGPLANEIPANLVRAFPRSLVGVTPQGWMRAWDGDHIVSYRYWVES
                     DQVLPHVQSCVMSVEDVRGDEEAISHFAALAPILVVTEGAAGSRVYWNGDVRFFRPPL
                     TQEVDPVGAGDVFAASFFIKLLQTRDPWEAARFATLVGANSVTRVGILGVPTPSELRA
                     YSTQIIAHPKQ"
     gene            complement(6943..7473)
                     /locus_tag="P5516_07775"
     CDS             complement(6943..7473)
                     /locus_tag="P5516_07775"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT92369.1"
                     /translation="MSLDPSFLKQLTLFAIGFAAAFLAALWLSLIFWASRDIRQRSRD
                     RFLRILAVVIVALLSIPGFLIYLILRPARTLEEEYQSALEEEALLQAIEESALCPGCN
                     RRIHADWLVCPSCNTVLKKTCKKCGKLLELPWNICPWCASAIPGTRKPTDKKKEEPEL
                     DEGFQPLLDEPLDTHP"
     gene            7589..8293
                     /gene="radC"
                     /locus_tag="P5516_07780"
     CDS             7589..8293
                     /gene="radC"
                     /locus_tag="P5516_07780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HRT92370.1"
                     /translation="MTRENGVTYRIKDLAEDERPRERLASIGAEFLSKAELLAILLRV
                     GTEGTSAVELGSQILKELGDLRGIQRASLAEMCRIRGLGPAKAAQIKAAIELGRRLTL
                     EAPQLAAGISSPADAAELVRYEMQALLQEELRVILLDTRNRKIDVKKVYTGSLNSSMV
                     RVGELFREAIQRSAASIILVHNHPSGDPSPSPEDTVLTRSVVQAGKLLDIEVLDHLVI
                     GQGSFVSMKEKGLGFE"
     gene            8357..8428
                     /locus_tag="P5516_07785"
     tRNA            8357..8428
                     /locus_tag="P5516_07785"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:8389..8391,aa:Met,seq:cat)
BASE COUNT         1964 a         1982 c         2403 g         2081 t
ORIGIN      
        1 gtgaaaaccg ggaaggattc agaggtctgg aaaaagccca gctttttata gagagataaa
       61 gaagcctggt tattatcctg ggtattcacg gtgatttgcc atattccccg ctgtgtgaaa
      121 tattccaaca gttgagcgac aagcgaatag ccgatattca ggtgcctgaa ttctggcagc
      181 actgccagcc gggcgagatg ggaggagaga gagctggcgg tcgagagctc gtaaccgatt
      241 atctgctcat ttacctcagc gacactggcg tgcgctgatt gcaggtacgc acgctccagt
      301 gaagcccgcg aaatgaccca ggcggcttca aaggtctgcc tgtccacttc tgctacctgg
      361 ttgaggtcag cgggttccat tggacgaatc agtacttcag ggggcatgtt tttcactgga
      421 ggtacctctc taccccagtg aagaacgacg atgttctcaa aaggcgtaaa gccagaggat
      481 cgcagcagcc tagtgtacca atcggacagg gacaatgaaa aaacctggca acgggttaca
      541 gccagttcga tcaaggcggg ttcaaacagc gagtgccaga cagcgttcac ctggttggac
      601 caatggtcca tgataaaagc gtgaatccag gcaacgcctt caggatctgg agcgcaggat
      661 aacagggcgg ttagctgttg atttgcgaag cgcagtaaaa aagggggctg tgaaacccac
      721 tccaccagcg ggcgccaatc caggtggcga tgagtcaggg aagctttatc gacaaaatca
      781 gtgattgccg tgatatcttg gctgacagcc tggcggattt cagtattcga gggtgcctgt
      841 agcatttact tgaataattt gttgcgaatg gctgccagcg ttgttttgaa cacggagtcc
      901 ttgatggtag gtttttctct ctggttatat gccgcttcca tggaagcaag ggcctggaag
      961 cctcgtttgg tcttcagttg taaatcagat acccggcgca gaatattgat tcgcagctct
     1021 ttctcgccgg cttctttgaa aatatccagt gtgtgttcgt aaagagccag tgcttcttcc
     1081 tttttatcca gcgcttcaag ggcagcggcg gtattcgcca gcgccatccc ctggcctttc
     1141 aaatcgccga ccttttggaa tgtctcatct gtgcctttga cgatatcata agcttcttgg
     1201 gcggcgttct ttcccagcaa ggcaacactg atattattgc gcatttctgc gacaagcagt
     1261 tcatcgttct gtttttcata gaactcaagg caggattgaa acccggcaat tgcggcatca
     1321 tattttcggc gattgtagtc tttaatcgcc tgttgtttaa gtttttcaat ttctgcagtc
     1381 attggtcaga atgtgatctc cagcaggttt tctgcgatag cgcgcagttt cgcggcatcc
     1441 atcccgctta gccttttcgc cagttcaagg tagggcaggt ttgccgggtt ttgaatgaag
     1501 gattgtactt cttcaggtaa ttgagatgaa tcctggcttg gtgacgcaat ggggggcggt
     1561 tcgctgtttt ggagggtgat tgaagaatat aaggatttca gtgggatgcc taattgagaa
     1621 caaaggtgtt caagtatagg tgcgggaatc gctgtttcgc ccgattcata cctggggaga
     1681 atcgctgcgc tgatacctac tgcagcggcc agctttttta ttgtccagtt ttgagccagg
     1741 cgctgttgtt tgagggttgc ggcgatgacc ctatttctga tttcaaggaa gttgttgacc
     1801 ttttcacgcg gcaggattgt cttcttgagt aaatctgggt tcccgctgag aagattttca
     1861 attggtattc gatacagctc agacaatacc tccagttgcg gcaggctggg agtagattga
     1921 ccgctttcgt aattcttgac cattttcacg gaaacaccca attgctcagc gactctggcg
     1981 agggtttttt cgctcacctc ccttgcggcc aataaccggg cgctgatgag ttgactttgt
     2041 tcttccagcg ggctcacttc catgttgact actccaatct caaggaacta tgaaaaccag
     2101 gcttgattat cgcatctggc ctttttcatt tctttgcttg ctcatcaagc gcgggcatct
     2161 ggtaacccat accggtgcga gtgactatgt gaacaggttc gtgggggtcg tcttccagct
     2221 tttggcgcag ccgggcgata ctcacccata ggatctcttt ttcatcttta tagccaggac
     2281 cccaagtagt ggtgagcaat tcttctgcgc tgagtacctt ccccaggtta tgggcgaaat
     2341 ggatcaacag cttgtattcc gtggcagaaa ggtacaccgg gtggtcgcct ttgaaaacct
     2401 cagccttggc gatgtcaatc ttcaggtcac catgcgtcaa tatccttgac ctaccagcgt
     2461 tttcttttaa ttggctgcgc cgtaaaacgg ctttgaccct tgccaccagt tcagtagctg
     2521 aaaagggctt cacgacgtaa tcatcagcgc cgaggttcaa cccgcgtaca cggtcgcgtt
     2581 cttctcccag ggcggtgagc atgatgatag gcacgttaga gaactgccgg atgcgggttg
     2641 ctgtggttat accgtcaagt tttggcatca tcacatccaa aatgatcagg tcaggcatct
     2701 cgtgtgagac caggtcaagc gcttcctgac cattcttggc ggttgcaatc aggtaacctt
     2761 cttgctcaag gttcacttta atcagccgta aatagagggg ttcatcgtca acaatcagta
     2821 tcctggcatt cataatcaat ccttatagac tcacatcgtg gttcaaagga agtgtgatgg
     2881 tgaattttat gccttctccc tgagaagaag agacgctaat ctctccgttg tggttgttga
     2941 tgatttgttt acagatagat aaacctaaac cggtaccata tacggatttt tccagctcag
     3001 gaacgcggaa aaaacgggag aacatgttgg gtagatattt ttcggggatc cctgggccgg
     3061 tgtcggaaaa ttcaataacc aggttggcct ctttttgaaa aaccttgatg gtaatcactg
     3121 agcccggagc gtactttttt gaattttcaa tcaggttgtc aatgacttgc gccaggcgac
     3181 gggggtcgcc gacaaccgtt ggcagttgct gttctaattc cagtttaatc acctgcgatg
     3241 ggttggtgta ttcagcgcgc ttgacctggt cgcggatgat ggcgtcaata tgcaggcttt
     3301 gaaagtcgaa acgcatgttg ccgctctgca agcgtgaaga atcgagcaaa tcatcaatca
     3361 attcgcccag atgccctgtc tcacggtcga taatttccag gaaatcatgc ttggtttttt
     3421 catcccagtc tgcatcttca cgcagcaagg ttgtggtata accgcggatg aatcccaggg
     3481 ggttgcgcag ctcatgggtg atggtgctga taaaatcatc ttgcaggttc gaggaacgac
     3541 gcagcccgct tagttctttc tcatagtcat ttattgtttt ttgacggatg acactggcaa
     3601 aaagcgtgcc cattaattca gcgaattcct tgctacccgg ttcgaaatca ggaccgccaa
     3661 aacggatgaa aataagcacg ccggtgattt cttttgaaat gctcagaggc acgccgagca
     3721 gaaaaggaga ctgtaagcgg tcagaagcgg ttttattctc aggttgaatg atgaaggctt
     3781 tcttttccgc aacaaccctg gaggcgatgt cttcccccca ggaaacgtcg gcttctttgg
     3841 atctgccgcg ccccatggcc cgagcgtaca gcacatccat ccgcttgatg gtgctatcac
     3901 ccaggtaaac gaccaggttg tcgaatatga aaaactcgcg gatgtaggtg accatcttgt
     3961 cgagtgttgc tttccagtcg tcagatgaag agacaaaatg gcaaaactgc aagagttttt
     4021 ctttatcgat ttcatcgaga agaaattcat tagacatgat taaccattat acaatattcg
     4081 tgccaactgg ttctcaattt tctcgctgaa tgcccggttt ttacgtaaac cttcgctttt
     4141 aaatgcgtgt agcgcgtgaa attcccccgc cagggtggaa aaccactgga aaacctttct
     4201 tctttagcta cagcgaggat gagaaccgcg tgtgtggctt aaaagatgga ttgaaacact
     4261 ctcgcgtttt aggggacatc attgcgcttc gagaaaactg gggtggtgtt ctgttgccga
     4321 aagagggcgc gttcttgcct ttaaagttct gccttgtaat gaaagcaact ctgttatcgt
     4381 tataggcaac gctgggtgag aaggtcttga ttgatcaggt tagtttttca atgagcccgt
     4441 tcagttcttc atccgaataa tagtgaatgg tgatggtgcc gccctttttt ccgctgcgca
     4501 gcgtaacacg ggtgcccagt ttaactcgta atcgttcttc gagttcttgg aagtgcgggt
     4561 caacttccac tggtggaagg gagcgaggtt tgatcccagt taacttgcgc accagctctt
     4621 ctgtttggcg gacgttgagc tgctgtgaga ggacggtgtg caaggccgag agctgtgcct
     4681 ggggagtagg caacccgagg agcgcgcgcg cgtgcccttc actgatgcgg cttgcagcaa
     4741 gcgcttcttg tgcttcggcg ggaaggttga gcaggcgcaa agtatttgtc acagtgacac
     4801 gacttttccc gacctggtca gccagggttt cgtgagtgag gttgaactcg tcaatcaggc
     4861 gcctgaagcc gcgagcggtt tcgagcggtg ataggtccgc tcgctggata ttttcgataa
     4921 gtgcctgggt cagacgctgt tgttcgctaa ccgtgcggat gatgataggc accttttcca
     4981 agccggcgat tttggcagcg cgcagacggc gctcgccggc gatgagcgtg tacattcccg
     5041 ttacagggtc gtgtgaaacg accaatggct gcaggacgcc gtgttcgcga atggaggagg
     5101 acaattctgc caggctttct ggcgagatag tgtcgcgcgg ctgttgaggg ttggggatga
     5161 tcatcgaaac cggcgcgtag gaatctgccc gggttggggt ggtgtttaac cctgcgggaa
     5221 ttaacgcgtc aagaccttta cctaatcctg ttttttgtgc catttatgcc acctgttcct
     5281 tgaagggcgg agagggttct ccatcctgag cgagaatttc gcgtgtcaac gccgcgtaag
     5341 cgctggcagc gcttgaactt ggcgcgtaat gcgaaatggg caacccgtaa gaaggtgcct
     5401 cagccaaccg taccgagcga ggtatgactg tatcatacac cagattgggg aagaacttac
     5461 gcacctcgtt gaccacatcg gttgaaaggt tggtgcggat atcgaacatg gtaaggatca
     5521 cgccgcggat gcgcatttca gggaagagag aggagcgcac caggttgagg gtttgggtga
     5581 gttgaccaat gccttccagc gccagatatt cacactgtac cgggataagc acgccatcac
     5641 gggcggcaat caatccgttc accgtgagca ggctgagaga aggagggcag tcaatgatga
     5701 tgtaatcata acgatcgagc gctggtttaa gcgcttcgcg catcctgaat tcacgccctt
     5761 ccatgccgat cagttccacc tctgagcctg ccaacgctgg agaggatggc agcagggcaa
     5821 tttttagcct ggggtttaag aggatgttag gcaagatggg ttgctctcca ataagcacct
     5881 cgtaagtgcc gttctttact tcgtatttat tgatgcccag cgaagaggtg gcgttggcct
     5941 gcgggtcaag gtcaatcagc agcacgcgct tactgaagag ccccaggtac gcgctgagat
     6001 tgattgcagt ggtggtcttg ccgacaccac ctttctggtt gacaagggta agcacgcgtg
     6061 tcattgcttt ggatgggcga taatctgggt ggaataagcg cggagctcag atggggttgg
     6121 aactcccagt atgccaacgc gggtgacaga attcgcccct accaaggtag caaacctggc
     6181 agcctcccag ggatcacgag tttgtaatag tttaatgaag aaagacgcag caaaaacatc
     6241 accggcgccg acagggtcca cttcttgcgt gagcggaggg cggaaaaaac gcacgtctcc
     6301 attccaatac acgcgcgaac cggctgcgcc ttcggtaacc accaggattg gcgcgagcgc
     6361 ggcgaaatgg ctgattgctt cttcatcccc gcgcacgtct tcaacgctca ttacgcagga
     6421 ctgaacatgg ggcaacacct ggtctgattc tacccagtaa cgataagaaa cgatgtgatc
     6481 gccatcccag gcacgcatcc agccttgagg ggttacgccc accaatgaac ggggaaacgc
     6541 acgcaccaga ttcgcgggta tttcattagc gagcgggcca aggtgaacaa taggcgcgga
     6601 acgccaggcg ggcggaacat cctccagtga aagcaggtca gccacatgca gaatgtgctg
     6661 gattctccct gtgggagtat aggtgtttcg aaaagtagag gtgaaagggg atggtttacg
     6721 cgcaatggcg atcccgttca gttctggcgg ctgaaggtca ggggagcagg aagtgacgat
     6781 gcccacgcgc aagccaaagg cgcgcgcggt cagcgcggcg taggaggctg tacctccaag
     6841 cgagagccct gcgggggtga catcttgcgt gatatgacca ataatgaggt aatcgacagg
     6901 ttcaatggtt gcgagatctg gcattgatgg ctttgtaccg gtctaggggt gggtgtcgag
     6961 tggctcatcc agtaacggct gaaaaccctc gtccagttcc ggttcctctt tcttcttatc
     7021 ggtgggcttg cgcgtacccg ggatggcgga ggcgcaccag gggcaaatgt tccagggcag
     7081 ctcaagcagt ttaccgcatt ttttgcaggt ctttttaagc accgtgttgc agctggggca
     7141 gacgagccaa tccgcatgga tacggcgatt gcagcccggg cagagcgcgg attcttcaat
     7201 ggcttgcagc aacgcttctt cttccaatgc gctttggtat tcttcttcaa gcgtgcgcgc
     7261 agggcgcagg atgaggtaaa tgaggaaacc ggggatggaa agcagcgcaa cgatcaccac
     7321 tgccagtatg cgcaaaaagc ggtcgcggct acgctggcgt atgtcacggc ttgcccagaa
     7381 gataagactg agccataagg cagccaggaa agccgccgcg aaaccaatgg caaacagagt
     7441 taactgctta aggaaggagg gatcaagact catacggggc tcctgtggga aattatagca
     7501 tggttgagaa acgaaggtga aagcgcttgc gtgaaagggg gtgttgttat ataaaacaag
     7561 cagttccaaa tgcagctagg aggaagaaat gacgcgtgaa aacggagtga cttaccgcat
     7621 taaagacctg gcggaagatg aaagaccgcg tgaacgcctg gcaagcattg gagccgaatt
     7681 tttgagcaaa gccgagctgc tggcgatatt actgcgtgta ggcacagaag gaacgagcgc
     7741 ggtggaactg ggctcacaga ttttaaagga attgggcgat ctgcgtggca ttcaaagggc
     7801 aagcctcgcg gagatgtgcc gcatccgggg gcttggaccg gccaaggcag cgcagatcaa
     7861 ggcggcaatt gagctgggtc ggaggttgac cctggaagcg ccgcagcttg ccgcgggcat
     7921 cagctctccg gcagatgcgg cggaactggt gcgctatgaa atgcaggcat tgctgcagga
     7981 agaactgcgc gtgattttat tggacacgcg caatcgcaag attgacgtga aaaaagttta
     8041 taccgggtcg ctcaattcgt cgatggtgcg ggtgggcgag ctgttccgtg aggccattca
     8101 gcgcagcgcg gcttcgatca ttttggtgca caatcacccc agcggggacc cttcacccag
     8161 ccccgaagac acggttttaa cgcgctcggt ggtgcaggcc ggtaagctgc tggatatcga
     8221 ggtgctcgac cacctggtga tcggtcaggg aagctttgtt tcgatgaaag aaaaaggact
     8281 gggatttgag taagagtaaa ttttcaaggg gttttgtttg acaaacaggg ttgattatag
     8341 taagataacc agcgctggcg aggtagctca atggtggagc agtggactca taagccattg
     8401 gatgtgggtt caaatcccac cctcgccact
//
LOCUS       DAOTLW010000056         8258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_8680_length_8258_cov_10.772157, whole genome shotgun sequence.
ACCESSION   DAOTLW010000056 DAOTLW010000000
VERSION     DAOTLW010000056.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 8258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8258
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_8680_length_8258_cov_10.772157"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(48..923)
                     /gene="mutM"
                     /locus_tag="P5516_07790"
     CDS             complement(48..923)
                     /gene="mutM"
                     /locus_tag="P5516_07790"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HRT92371.1"
                     /translation="MPELPEVETIARFLRGTHGLAISRSPHPNGPLGVLGRKFADVQV
                     TWQRSIAEPAAPAFCARLYGQSILEITRRGKFLVFQLEKDWMLIHLRMSGDIRVEPRD
                     ENPPGLHDRIIFNFADDARMVFNDTRKFGRAWLLKDPQQVLGNLGPEPFDPELTAGVF
                     HRMLNEKKRAIKPLLMDQKFLAGMGNIYTDEALHLARVHPLEKASSLNYSQAESLLRA
                     IREVLTEGIRRNGASIDWAYRGGDSRITSGCISAMARNAPCAATISNESWSASGGLIF
                     VLLASCWKKAKKTLL"
     gene            complement(939..1934)
                     /locus_tag="P5516_07795"
     CDS             complement(939..1934)
                     /locus_tag="P5516_07795"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HRT92372.1"
                     /translation="MPVIFSARHERMKYGDIKLYSGTSCPELANRIATHMGLELGERN
                     IVQFPNENLFIQLRSSVRGQDCYAIQTTAAPVHRNLMELLILIQTLRLDSAARVTAVV
                     PYLCYARSDKKDQPRIPITARLVADMIEAAGADRYMFLDLHAGQIQGFFSIPGDVLTA
                     FYILSDYLKVKKSKMHKPVVVTADLGFAKKARNFAVSMDVPLAFIEKRRISNDSKAQA
                     LSMIGDVRERDVVIVDDEVDTGGSMIQAVNLAKESGARDVYMVFVHPVLSANAVENLA
                     ALPVTEFITTDTIPIPPAKFKKFGKRMTVLSIAPLLGEVILRAHEGRSVGELFNE"
     gene            complement(1941..2489)
                     /locus_tag="P5516_07800"
     CDS             complement(1941..2489)
                     /locus_tag="P5516_07800"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HRT92373.1"
                     /translation="MILGDGIRLRAIEKADIPRFVRWLNDPEVTRFLSFFSPLSTAME
                     ENWFEQQLKSSPAEGQVFSIEVQAGDDWIHIGNTGLHQIESINRSAEFGIFIGETGYH
                     QKGLGLAATRLTVKHGFENLNLNRIYLYVCEANSRAINTYEKAGFVHEGVLREAVFKQ
                     GCYENLVLMSVLKSEWVSLPTK"
     gene            complement(2508..3827)
                     /gene="aspS"
                     /locus_tag="P5516_07805"
     CDS             complement(2508..3827)
                     /gene="aspS"
                     /locus_tag="P5516_07805"
                     /EC_number="6.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF003483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004815 - aspartate-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006422 - aspartyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA(Asn) ligase"
                     /protein_id="HRT92374.1"
                     /translation="MERTLIKDLRGKIDQTVKLQGWLQTLRDQKKMQFLILRDPTGLV
                     QVAHWKPNDEILASNIASLGAETALTIIGKVIDNPVVKLNQLEVQLESLVVENLAESP
                     LPFDPFAETLPALDYRLDWRYMDLRRAYNNLLFQVQTTMEMAMREYWLKHDFIEIHSP
                     KLMGTPSESGAELFSLDYFERKAYLAQSPQFYKQMAMAAGFERIFEIGPVFRADPSFT
                     ARHMTEFTGVDLEISWIDSHEDVMAFEENWLQYVYARVKELHGEAIKETFGVELVVPQ
                     VPFPRIPMRDAVEILKSRGYVLPADKKGDIDPGGEREIASYVKEKYDHDFVFLTDWPI
                     GVRPFYHMRLAGDETLTRSFDLIANGLEITTGAQREHRPEILAKQALEKGLHLEPIQY
                     YIDFFRYGCPPHGGFGLGLSRLLMVMLNLPNIREAVYIFRGPTRLNP"
     gene            complement(3859..>4274)
                     /locus_tag="P5516_07810"
     CDS             complement(3859..>4274)
                     /locus_tag="P5516_07810"
                     /inference="COORDINATES: protein motif:HMM:NF015279.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92375.1"
                     /translation="GAENTARALRANLDLGYHLHMNHNGAHRMTIKENEVRNTPAWTP
                     PIIQTIATQHNGIDEVVAEILKHREYLIDTGLWQKREQDRLRSEVYHLLQTGLVKRWQ
                     QSTPPQVFERLLQKVFARELSPRAAIDSLLNGSYN"
     assembly_gap    4275..4374
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<4375..5012)
                     /gene="meaB"
                     /locus_tag="P5516_07815"
     CDS             complement(<4375..5012)
                     /gene="meaB"
                     /locus_tag="P5516_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HRT92376.1"
                     /translation="MVDLDAVTRGNRLELARLLTEVENQTPAGEQALDRLFARAGKAH
                     LVGITGAPGTGKSSLVSCLAKNIRVAQKQTVAIVAVDPSSPFTGGAILGDRVRMRELA
                     GDPGVFIRSMASRGELGGLASATAGVVQVLDAAGFDFILIETVGAGQSEVDIACLAYT
                     TLVVESPGLGDDIQAIKAGILEIADILVVNKSDLPGAENTARALRANLDLGY"
     gene            complement(5028..5429)
                     /locus_tag="P5516_07820"
     CDS             complement(5028..5429)
                     /locus_tag="P5516_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HRT92377.1"
                     /translation="MTEKKIRVLIAKPGLDGHDRGAKVVARALRDAGMEVIYTGLRQS
                     PEMIAEAALQEDVNVIGLSILSGAHLVLVPRVIDLIRANGQEEVKVFVGGIIPEEDIP
                     VLTQAGVSAIYGPGTPTTKVVQDIRDAFSAV"
     gene            6372..7001
                     /locus_tag="P5516_07825"
     CDS             6372..7001
                     /locus_tag="P5516_07825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92378.1"
                     /translation="MTYERKGYVHGAWVIKIGQNDLVIKASENRSFPELDQLYTPRVK
                     NPKHWDDYAEKLIPDAEKQLYSLLDLPYYPVEKRVKLSQLIERTKWKFAWTYARTYPH
                     EYTTKALCEAADHAKLIDCIEKYGIIQRFGESYRKYFYFEDRKYWHMGEPYSDDPELR
                     PNVINRTWVDVRRHAENVKHRWTVEEVELQQRLWEIQLEKSTDRNKPKK"
     gene            complement(7061..8143)
                     /locus_tag="P5516_07830"
     CDS             complement(7061..8143)
                     /locus_tag="P5516_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92379.1"
                     /translation="MFVGGEFYYDSSWQVNTSVVDTSHLTFLNGGKACLIVIGDYLRA
                     HGINEILLPSYLCPSILNTLEKHGLGCDFYPVQPDLTIDLEEMARKAEGHRAVYFIHY
                     FGCPREPAELDLLRSLQAGGTLLIEDNAQCGFSPTVCGDFAFNSMRKLCAYDGGYLDS
                     RFDLLPYIEKRAGLKNRRLPLIRDYRSRLADYLFNSKGDRNELQQLYELAENHYESDE
                     VVEGDIVERHHIEHLDWDGIRKKRRDNYRYLVSLVEGLPGITPIFPILPQGVTPLSLP
                     VYVNIPSRDQLGDALGDAGIGLTIHWEGLVRDPRVSGNTAVVDMASRMLSLVIDQRFS
                     HKHMDYQAQTLAEWVNRHQSPSYPRA"
BASE COUNT         2061 a         2138 c         2031 g         1928 t
ORIGIN      
        1 caataacacc atcattatcc aatgaaaaag taaataatgc aataacccta caataatgtt
       61 tttttagcct ttttccagca gctggcaaga aggacaaaaa tgagtccccc gctggctgac
      121 catgattcgt tcgatattgt cgccgcacaa ggggcatttc tcgccattgc gctgatacac
      181 cctgaagtaa ttctggaatc cccgccgcgg taagcccagt cgatgctggc gccgttccgc
      241 cgaatacctt ctgttagcac ctcgcggatc gcccgcagca gggattctgc ctggctgtag
      301 ttgagggatg acgccttctc aagcgggtgt acgcgggcga ggtgcagcgc ctcgtcggtg
      361 tatatatttc ccattccagc caggaacttt tgatccatga gaagcggttt gatagcccgc
      421 ttcttctcat ttaacatacg gtgaaagacg cctgccgtca gctcagggtc aaagggttct
      481 ggtccaaggt tacccagaac ttgctgaggg tctttgagca gccaggcccg gccgaatttg
      541 cgtgtgtcgt taaaaaccat gcgcgcgtca tccgcaaaat taaaaataat cctgtcgtgc
      601 agccccgggg gattctcatc acggggttcc acccggatat ctccactcat gcgcaggtgg
      661 atgagcatcc aatctttttc aagttgaaac actaaaaatt tccccctgcg tgtaatttcc
      721 aaaatgcttt gcccgtaaag gcgcgcgcaa aacgcagggg cagccggttc ggcgatagaa
      781 cgctgccagg tcacctggac atcggcgaat ttcctcccca gcacccccag cgggccattc
      841 gggtgcggtg agcgtgaaat cgccaggccg tgcgttcccc gcaaaaacct ggcgatggtt
      901 tcaacttcgg gtaattcagg catatggtta taaaaagcct attcgttgaa caattcgccc
      961 acactgcgcc cttcgtgggc acgtaagatc acctctccca gaagaggagc gatagaaagg
     1021 acggtcatac gtttaccgaa tttcttaaat ttcgccggcg gaatgggaat ggtatccgtg
     1081 gtgatgaatt cggtgaccgg tagggcagcc aggttttcaa ccgcgttggc tgaaagcacc
     1141 ggatggacaa aaaccatata cacgtctcgg gcgcctgatt ctttggcgag gttcaccgcc
     1201 tgaatcatcg acccgccggt atccacttcg tcatccacaa tcaccacatc ccgttcacgc
     1261 acatcaccaa tcatagaaag cgcctgagct tttgaatcat tgctgatacg ccgtttctcg
     1321 atgaacgcca gaggcacatc cattgagacc gcaaaattgc gcgctttctt ggcaaaacca
     1381 aggtcagcgg tcaccaccac gggtttgtgc atttttgact ttttcacctt caggtaatcg
     1441 ctcaagatat agaacgcagt gagcacatct ccgggaatgg agaagaatcc ctgaatttgc
     1501 ccggcgtgca ggtcaagaaa catgtaacgg tccgcgccgg ccgcttctat catatccgcc
     1561 accaggcgcg cggtgatagg aattcttggc tgatcctttt tatctgagcg cgcataacac
     1621 aggtaaggca ccacagcggt cacgcgggca gcagaatcga gacgcagggt ttggatgaga
     1681 atcagcaact ccatcaggtt gcggtgtacc ggcgcggcgg tggtttgaat ggcataacaa
     1741 tcctgccctc gcacgctgga acgcaattga atgaacaagt tctcattagg gaactgaacg
     1801 atgttgcgct ctccaagttc caaccccata tgggtggcaa tacgattggc gagttcaggg
     1861 caagatgtac ccgaatagag tttgatatct ccatatttca tgcgttcatg acgagcagaa
     1921 aagattactg gcatatatcc tcactttgtg ggtagactca cccattcaga tttcagaaca
     1981 gacatcagca ccaggttctc atagcaacct tgcttgaaga ctgcctcccg aaggacaccc
     2041 tcgtgcacaa aaccggcttt ttcgtaggtg tttatcgcgc gcgagttggc ttcgcaaaca
     2101 tacaggtaaa tgcgattcaa attgaggttt tcaaaaccat gcttcacggt cagccgggtt
     2161 gccgcgagtc ctaacccctt ttggtggtaa ccggtctcac caataaaaat gccgaattcc
     2221 gcgctgcggt tgatggattc tatttggtgc aaaccagtgt taccaatgtg aatccaatcg
     2281 tctcccgcct gaacctcgat agagaacacc tggccttcag caggcgaaga tttcagttgc
     2341 tgttcaaacc agttctcctc catcgccgtg gaaagcggag agaaaaaaga caaaaagcgg
     2401 gtcacctcag ggtcattcaa ccagcgtaca aagcgcggga tatcagcctt ttcgattgcc
     2461 cgtaaacgta ttccatctcc cagtatcatc tctgcacttt tctgcgacta agggttcagc
     2521 cgggtaggtc ctctaaagat gtacaccgct tcgcgtatgt taggcaggtt caacatcacc
     2581 atcaacaggc gtgaaagccc caatccaaac ccgccatgcg gcgggcagcc atagcggaaa
     2641 aagtcaatgt agtactggat aggctcgagg tgcagtccct tctccagcgc ctgtttggcc
     2701 aatatttctg ggcggtgttc gcgctgtgca ccagtagtga tttcaagccc attggcaatc
     2761 agatcaaaac tgcgcgtgag ggtctcgtct cctgccaggc gcatgtggta gaaaggacgc
     2821 acaccaatcg gccagtccgt caggaaaaca aaatcgtgat cgtatttctc ttttacataa
     2881 ctcgcgattt cgcgttcacc gccgggatca atatcaccct tcttatccgc gggcaagaca
     2941 tacccgcgag attttaatat ctctaccgcg tcacgcatcg gaatgcgcgg gaaaggcacc
     3001 tgtggaacca ccagctcgac gccgaaggtc tctttaattg cctctccgtg gagctccttt
     3061 accctggcat aaacatattg cagccagttc tcctcgaacg ccatcacatc ttcatgtgaa
     3121 tcgatccagg aaatttccag gtcaacacca gtaaactcgg tcatatggcg ggcagtaaag
     3181 gaaggatcag ccctgaacac cggccctatc tcaaagatgc gttcaaaacc agccgccatg
     3241 gccatctgtt tgtaaaactg aggagattgc gccaggtagg ctttgcgctc aaaataatcc
     3301 agcgaaaaga gttccgctcc gctttcgctg ggtgtgccca tcaattttgg cgaatggatt
     3361 tcaataaagt catgcttcag ccagtattca cgcattgcca tttccatggt ggtttgcacc
     3421 tggaaaagca ggttattgta ggcacggcgc aggtccatat aacgccagtc cagccggtaa
     3481 tccaacgcgg gtaaggtttc ggcaaatggg tcaaaaggca acgggctttc agccaggttt
     3541 tcgaccacca gtgactccag ctgcacctcg agctgattca gtttaaccac cggattgtcg
     3601 atgaccttgc caataatggt cagagcggtt tcagccccca agcttgcaat atttgatgcc
     3661 aggatttcat cattgggttt ccagtgagcc acctgcacca ggcctgtggg gtcacgtaag
     3721 atgagaaatt gcatcttttt ttggtctctt agcgtctgca gccagccctg cagcttaact
     3781 gtctggtcga tcttcccccg caggtcttta atcaatgtcc gttccattgt tcctcactta
     3841 cctgatagga tgtaaagcct aattataaga gccattgagc aatgaatcaa tcgctgcgcg
     3901 gggcgaaagt tcacgtgcaa atactttctg taacaatctt tcgaaaacct gaggtggggt
     3961 tgactgttgc cagcgtttca ccagcccggt ctgcagcagg tgataaacct cactgcgcaa
     4021 tctgtcctgc tcacgctttt gccacaaacc agtatcaatt aaatattcgc gatgcttgag
     4081 aatttcggcg acgacttcat caatcccatt gtgctgggtc gctatcgttt ggataatggg
     4141 aggggtccag gcgggcgtat tcctgacttc attctcttta attgtcatcc tgtgagcgcc
     4201 attgtggttc atgtgcagat gataaccaag gtcgaggttg gcgcgcaacg cgcgcgcggt
     4261 attctcagcg cctgnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     4321 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnntgataa
     4381 ccaaggtcga ggttggcgcg caacgcgcgc gcggtattct cagcgcctgg caggtcggat
     4441 ttgttgacca ccagaatatc ggcaatctcg aggataccag ccttgatcgc ctggatatca
     4501 tcgcccaatc ccggggattc caccaccagg gtggtgtacg ctaggcaggc gatatctacc
     4561 tcgctttgcc cggctcccac ggtttcgata aggatgaaat caaaccccgc tgcgtccagc
     4621 acctgcacca cgccagcggt ggcgctcgcc aggccgccca gctcgccgcg cgaagccatc
     4681 gaacggataa atacacccgg gtcgcctgcc agttcgcgca tgcgtacacg gtcccccaat
     4741 atcgcgccgc cggtgaaggg gctggaaggg tcaaccgcca caattgcgac cgtttgtttt
     4801 tgcgcaacac ggatgttttt tgccaggcag ctcaccaagg atgatttgcc cgtcccgggc
     4861 gcaccggtga tgcccaccag gtgcgccttg cctgcgcgag cgaacaaccg atccaacgcc
     4921 tgttcaccgg caggcgtctg gttttccacc tcggtcaaca agcgggcaag ttcaaggcga
     4981 ttaccgcggg taaccgcgtc caggtctacc atctcactcc ccgcaactca gacggctgaa
     5041 aaggcgtcgc gaatatcctg aacgactttc gtggtcggtg ttccaggtcc gtaaatggca
     5101 ctgacaccag cttgggtgag aacagggatg tcttcttcag ggatgatgcc gccaacaaac
     5161 accttaacct cttcctgccc atttgcgcgt atcaggtcga tcaccctggg aacaagcacg
     5221 aggtgcgcgc ctgaaaggat agaaagacca ataacattca catcttcctg cagggcagcc
     5281 tcggcgatca tttcgggcga ttgccttaac ccggtataaa tgacctccat accggcgtct
     5341 ctgagcgcgc gtgccaccac cttcgctccc cggtcatgtc cgtccagccc gggcttggca
     5401 atcaagacac gtatcttttt ttctgtcata gacatctcct tggtgtttgc actggtactc
     5461 ttccatctct aattctacat tcatcagccg aaatctgctg tcttttttcg ttattggggc
     5521 aaacttatat cctgttttgg agaatttaaa agttcaagcg cgtgggagac agaaaggcca
     5581 atcaaaaaag aacctcgaga aaaagaaagt aagggaagaa gaaatcaact ggctaccgag
     5641 agaatctcaa gcgacacgat agtggtgcgt aagaggttgc aagagaacga gctgaaggag
     5701 ttggaagaaa ggagctgctg accacgtctg atgcgccaac cgttattgtg gagaacagta
     5761 ctgtttgggc gagtgctgga gttacgggag caattaccgt gtaggaaaaa ggataagcgg
     5821 gtcgtcctgg cacaacgtca agaggaaaga gttacaggct ctactacggg atgaatacgg
     5881 gcagaccaga ggagaaaaag agaaaaagaa ggccatatgc catacgaaaa ccaaaatata
     5941 caggtcaatg aacctggtga cccggtacag atgcatactt tatcgacctt aataaataag
     6001 gaacggttct ataaccagat ccgacctcat caggcactgg actatctcac cccagccgag
     6061 tatattaata aatatcaccc tgaagtaacc tcggaaaaat ctcatatgta ttgaacgggt
     6121 acaatctctt gaatcgtctc tccaaaaggt tataatcatt ttggtgagtc atttccagtc
     6181 tccttgtatg gtttagtcac cttaaggata cttctgactc accacttttg ttatagttct
     6241 acattttcca gaaaggatga tgcatcaacg ttgaaaatcc aattgaagta tttcatgagc
     6301 ccttaaggag gagaaatgga atttcgggcg tatgatctag ttcggtcaat ggttgagcgc
     6361 ctgggggcac aatgacctat gagcgtaaag gttacgttca cggtgcctgg gtgatcaaga
     6421 tcgggcaaaa tgatttggtg atcaaagcga gtgagaacag atcttttccg gaactggatc
     6481 agttatatac tccacgggta aagaacccga agcattggga tgactatgca gagaaactca
     6541 ttccagatgc agaaaagcag ttgtattcat tgttagatct tccatattat ccagttgaaa
     6601 agcgcgtaaa actttctcaa cttatcgagc gtacgaaatg gaagttcgcc tggacctatg
     6661 cccgcaccta tcctcacgaa tacaccacaa aagccctttg tgaagctgct gatcacgcga
     6721 agcttattga ttgtattgaa aagtatggga tcattcaacg attcggcgag tcttaccgta
     6781 agtatttcta ctttgaagac cgtaaatact ggcacatggg cgaaccatac tctgacgacc
     6841 cggaattgcg accaaatgtg ataaaccgca cttgggttga tgtcaggcgg catgctgaaa
     6901 atgtcaagca tcgatggact gtggaagagg ttgaacttca gcagcgatta tgggagattc
     6961 aactagaaaa atctacagac cgaaacaagc caaagaagta aaaattaatg cgataaccca
     7021 tttaattgcg ctgtgtctcg aactcacctc accaaggttc tcaagcacgc ggatatgacg
     7081 ggctctgatg gcgattcacc cattctgcca gggtctgcgc ctggtaatcc atatgcttgt
     7141 gcgagaagcg ctgatcgatg acaagcgaca gcatcctgga ggccatatcc actaccgccg
     7201 tgttaccaga aacacgcggg tcgcgtacga gcccctccca gtggatggtc agaccgatac
     7261 cggcgtctcc cagcgcgtca cccagttggt ctcgtgaggg gatgttgaca tacaccggca
     7321 ggctcaaggg cgtgactccc tggggcagga tcggaaagat cggggtgatc cccggcagtc
     7381 cttctaccaa actcacgagg tagcgataat tatcgcgccg ttttttcctg atgccatccc
     7441 aatcgaggtg ctcgatgtga tggcgctcaa cgatatcccc ttccaccacc tcgtctgact
     7501 cataatggtt ttcagcgagt tcatagagct gctggagttc attgcgatct cccttggagt
     7561 tgaaaagata atcagccagg cgtgagcggt agtcccggat gaggggcaat ctcctgttct
     7621 tgagcccagc ccttttttcg atataaggca gcaggtcgaa ccgcgagtcc agatacccgc
     7681 catcataggc gcacaacttg cgcatactgt tgaaggcaaa gtcgccacag acagttggac
     7741 tgaatccaca ttgcgcgtta tcctcgatca gcagggtgcc gcccgcctgg agggagcgca
     7801 gcaggtcgag ctcagccggc tcacggggac aaccgaaata atggatgaag tagaccgccc
     7861 ggtgaccctc cgcttttcga gccatctcct cgaggtcaat tgtcaggtca ggctgcaccg
     7921 gataaaagtc gcaccccagc ccgtgttttt ccagcgtatt gaggatggag ggacaaagat
     7981 aggagggcag cagtatttca ttaattccat gcgcgcgcag gtaatcacca atcactatca
     8041 agcaggcttt ccccccgttg aggaaggtca aatgggaggt gtctacgacg gaggtgttca
     8101 cctgccaaga agaatcataa taaaattctc cacccacaaa cataatccct gctttttcaa
     8161 taaaaataaa ctaatctttt ctattgtata gtgatttata cgggaattaa tgattttcgt
     8221 ttattgcaat aatcgcaaga gcgggttgcg cccgggtt
//
LOCUS       DAOTLW010000057         7894 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_9092_length_7894_cov_11.720628, whole genome shotgun sequence.
ACCESSION   DAOTLW010000057 DAOTLW010000000
VERSION     DAOTLW010000057.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7894)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7894)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7894
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9092_length_7894_cov_11.720628"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..532
                     /locus_tag="P5516_07835"
     CDS             <1..532
                     /locus_tag="P5516_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92380.1"
                     /translation="QTSAGLPGRRRSLAIPSLLATFLVLGLILPVLNLIPGQLPEETD
                     PTIEGKAYATLSQRGLVSADEWNAFLQDPNHILVQGRAYHPRYYRGNFFRPGNLSFEL
                     MLLAKDHVLVGYSTGIIPREAFADGSDVIFVGCLIQRDTLWAAQREIVESIAIIQLDH
                     EASLLFDEKTGWRCNR"
     gene            complement(649..1800)
                     /locus_tag="P5516_07840"
     CDS             complement(649..1800)
                     /locus_tag="P5516_07840"
                     /inference="COORDINATES: protein motif:HMM:NF015412.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92381.1"
                     /translation="MKNKIRIGVIGTGFIANGLMKAIECQPDMVVSAVLTRRPLSDFP
                     LSELHTQSVDELLNKSQVIIECAGDPIYGTEVVARALEASLPVITMDAELQVTSGTYL
                     STLGVLTEAEGDQPGALAALKREALAMGFKPLVYGNLKGFLNHLPTMEDMQYWAKVQG
                     ISLDQVTAFTDGTKLQLEQVLVANGLGATIACQGLLGYSSADVEEGSYRLAAQAKNLG
                     QPISDYLLSQPGAPKKLPAGVFITGEHDPRQAPSLKYLKLGEGPFYTLLRNYHLCHLE
                     IPKTIREVMQGGQVLLDNSSKPSTSVVAIAKTALKPGQFINQALGSFEVRGEAILINT
                     MPDHLPIGLMRNARIRHHIEPGQLLTMGDVDLPNSLALKAWQYTLHQSQ"
     gene            2037..3011
                     /locus_tag="P5516_07845"
     CDS             2037..3011
                     /locus_tag="P5516_07845"
                     /inference="COORDINATES: protein motif:HMM:NF027681.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT92382.1"
                     /translation="MKKALITGITGQDGSYLAEFLLHKGYDVHGLVLRNEIEDPERSL
                     TRLKNCMNDLVLHVGSIESYPSLQAIFEKEQPDECYHLAAYSFVSYSFDEEFTVFNTN
                     IHGTHNVLSALKNSSPGCKFYFAGSSELFGRAKKSPQNEQTPFNPRSAYGITKVTGYY
                     LTANYRENYGLYACTGIAYNHESPRRGFEYVTRKISRGVALIKAGLQEKLVLGNLEAI
                     RDWGYAPDYVQAMWKMLQQDTPHDFVIATGIGHTIREFCQLAFERVGLDYRDFVSFDP
                     KFYRPVEEVPLIGDATRAAADLNWSPEVSFKELVHLMVDADIEQIESI"
     gene            3212..4042
                     /locus_tag="P5516_07850"
     CDS             3212..4042
                     /locus_tag="P5516_07850"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92383.1"
                     /translation="MVRSKNQVKIYDSEKVKWVAIEEIKAVWFYRDLMVQLVKRDIVA
                     RYKRSTLGVLWTMLNPLATMIILTIVFSRFFNMRSVYPAYIITGLITWSFFSQTILFS
                     LNTTLRGSDLYNKIYLPQTAFVISVLGGGILNYLLSIIPMALIFLVTKVPIHPTVLLF
                     PVGLLLLATFTLGLGLLLSTGVVFFPDIAEFAPVILQAWFYITPIIYPAELQLDIMGG
                     LFLKINPMYYFIKLIRNLLYDGVIPSGLDWLIAFLMAILMLVIGWWVFTSKSKRFGYQ
                     "
     gene            4047..4814
                     /locus_tag="P5516_07855"
     CDS             4047..4814
                     /locus_tag="P5516_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92384.1"
                     /translation="MTLNDPEEQTAVFLKDVSVAYRLPVERFASIKEYAIKKVLGKVH
                     YKDFFALNDINLSINRGEIFGIIGRNGAGKSTLLKVISRVLYPTTGRVWVKGIVAPLL
                     QLGAGFHPELTGYENIYLNGTLLGHKRVEIKDKIDAILAFSEIGDFINSPIRTYSSGM
                     VARLGFSIATAWVPDILILDEVLSVGDAAFREKCSNRMQELIKSGATVFLVTHDAQLI
                     SELCTRAVLLENGHIVSSGTGAEVSVIYSRSLADAYS"
     gene            4830..5378
                     /gene="rfbC"
                     /locus_tag="P5516_07860"
     CDS             4830..5378
                     /gene="rfbC"
                     /locus_tag="P5516_07860"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001764656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRT92385.1"
                     /translation="MKFTPTRLADVLLIEPRVFGDERGFFMETFQQERFKNAGIDQKF
                     VQDNQSRSIRGTLRGLHYQIKHAQGKLVRVISGEIFDVAVDLRRSSPTFGKWVGCNLT
                     AENKHMLWIPPGFGHAFYTLSDWADVVYKATDFYSQPDERTIIWNDPEIGIQWPEQVQ
                     QTLILSEKDARGVSFLQAEVFL"
     gene            5375..6463
                     /gene="rfbB"
                     /locus_tag="P5516_07865"
     CDS             5375..6463
                     /gene="rfbB"
                     /locus_tag="P5516_07865"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007846915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HRT92386.1"
                     /translation="MNFSRVLVTGGAGFIGSNFIYYTLKHFPDVFILNLDLLTYAGSL
                     ENLKDIPAEARYQFVQADICDQQTVTELIRAYNIDAVVHFAAESHVDRSILGPAAFMN
                     TNILGTFSLLEAARAVWLAEKKLKPEQVRFHHISTDEVFGSLEPGAPAFSETTPYSPN
                     SPYAASKASSDHLVRAYHHTYNLPVTLTNCSNNYGPYQFPEKLIPLMVLNALEGKALP
                     IYGDGMQVRDWLYVEDHCEAIWKVLQEGKVGESYNVGGNNQPPNIEIVDAICALMDEF
                     LPDSEFRPHQKLKKFVTDRPGHDRRYAMNTSKIERELGWKPRHDVEKGLRKTVQWYLE
                     NKAWINSIRAQKQYEEWMVKNYTKRGEK"
     gene            6460..7332
                     /gene="rfbA"
                     /locus_tag="P5516_07870"
     CDS             6460..7332
                     /gene="rfbA"
                     /locus_tag="P5516_07870"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002221803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRT92387.1"
                     /translation="MKGIILAGGKGTRLFPLTLSTSKQLLPVFDKPMIYYPLSMLMLA
                     RIREILVISSPEDLPSFRRLLGDGSQWGLEFAYAEQVEPRGLADAFIVGEKFINGEPC
                     CLILGDNIFFGQGLPEKLRESAALTRGAIVFAYPVRDPERYGVVEFDEQGKAISLEEK
                     PANPRSKFAVPGLYFYDEKVVMMAKSLKPSARGEIEITDLNRMYMERGELNVHQMSRG
                     TAWLDAGTHESLLQASNFIQAVEERQGIMISCPEEVAYRMGFIDKDQLLKLAKIYESN
                     SYGNYLKQFIESVS"
     gene            7329..>7894
                     /locus_tag="P5516_07875"
     CDS             7329..>7894
                     /locus_tag="P5516_07875"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HRT92388.1"
                     /translation="MKFLLIGKYGQLGWELMRTCMTLGEIVAVDYPEVDLANSHALRE
                     LVQTVKPDILLNPAAYTAVEKAESEFEVARLVNGVAPGVLAEEMKKLGGALIHYSTDY
                     VFDGEKGSSYIENDAPNPINAYGVTKLEGEQQVAAVEGTYLIFRTSWVYSLRRAGFIS
                     KVLNWARTQEVMRIVDDQIGSPTWARMLA"
BASE COUNT         1963 a         1833 c         2017 g         2081 t
ORIGIN      
        1 gcagaccagc gctggtttac cgggcagacg gcgcagtttg gctatcccct cgctgctagc
       61 aacattcctg gtgctggggt taatcttacc ggtcctcaac cttatccctg gacaacttcc
      121 agaagagacc gatcctacga ttgaggggaa agcgtacgcc acactttcgc agcgcgggtt
      181 ggtaagtgct gatgagtgga acgcgttcct gcaagaccca aatcacatcc ttgttcaagg
      241 cagggcgtat cacccgcgct attatcgagg taactttttc cggccgggca accttagttt
      301 tgagttgatg ttgctagcca aagaccatgt actggtgggt tactcaacag ggatcatccc
      361 cagggaagct ttcgctgatg gcagtgatgt tatctttgtg ggttgcctta tccagcgcga
      421 tactttatgg gcagcccaac gcgaaatcgt agaatccatc gccatcattc aactggacca
      481 tgaagctagc ctgttatttg acgagaagac aggttggcgc tgcaatcgct gacttagcag
      541 aaaaaccggt ggattgtatg cgcgttcttc ttgaagcgcg ggattaactg tttgattgat
      601 gtggacggtc agccgcggag gattttttca caatcagagc gataggtttt attgcgattg
      661 gtggagtgtg tactgccagg cttttaaggc taaagagttt ggcaggtcta catcacccat
      721 ggtaagcagt tgacccggtt cgatatggtg cctaatcctt gcgttacgca tgagaccaat
      781 cggaagatga tcaggcatag tattaatcaa gatggcttcc ccccgaactt caaagctgcc
      841 caaggcttgg ttgatgaact gacctggttt taacgctgtc ttggcaatgg ccaccacact
      901 ggtggacggt ttgctgctgt tatccagcag cacctgacca ccttgcatca cttcgcggat
      961 tgttttgggt atttcaaggt ggcaaagatg ataattccgt aacaatgtgt agaatggacc
     1021 ttcgccaagt ttgaggtatt ttagagacgg agcctgccgc ggatcatgtt caccggtgat
     1081 gaatacgcca gcaggcagtt tttttggcgc gcctggttga gagagcaaat aatcgctgat
     1141 tggctggccc aagtttttcg cctgggctgc taatcgatag gatccttctt ctacatccgc
     1201 agaagaatac ccaagcaatc cctggcaggc gatggtcgcg ccgagcccgt tggctaccag
     1261 aacctgttcc agttggagct tcgtgccatc ggtaaaagcg gtcacctggt ccaggctgat
     1321 gccttgaact tttgcccagt attgcatgtc ttccattgtt ggcaaatggt tgaggaaccc
     1381 ttttaaattg ccataaacca gcggcttaaa tcccatagcc agggcttcac gttttagggc
     1441 ggccagggcg cctggctggt ccccttccgc ttcggtgagt actcctaatg tggaaaggta
     1501 tgttcccgaa gtgacctgaa gctcagcgtc catggtgatg acaggtagcg acgcctccaa
     1561 tgcgcgggcc acgacctcag tgccgtaaat agggtccccg gcacattcga taatcacctg
     1621 gcttttattc aataattcat ctacagattg ggtgtgcaac tcgctgaggg gaaaatcact
     1681 cagaggcctg cgggtgagaa ccgcggagac taccatgtct ggctggcact cgatcgcctt
     1741 catcagccca tttgcaatga aacctgttcc aatgacaccg attcgaattt tgttcttcaa
     1801 attctcacct ttctgaagac ctgctgtttc cgggttgatc aatgcattat actctacaaa
     1861 taattggctt caacgaaggc tggcgtgcac tgggggttgg aaaaaatcgt cgcactgaag
     1921 atcgaccgaa tggcttgatg tgtgcttcac ctcgttgttt ttgaattaat gaatgtacaa
     1981 tggtattaat ttgagtattc aaatggtcga tgagatgatc gaatgactga gggatgatga
     2041 aaaaagcgct tatcacggga attaccgggc aagatggttc gtacctggct gagtttttac
     2101 tgcacaaggg gtatgacgtt catgggcttg tgttgcgtaa tgaaattgaa gacccggaac
     2161 gttcattgac gagattaaaa aactgcatga acgatcttgt cctgcacgtg ggatcgatcg
     2221 agtcctaccc atccttgcag gcgatcttcg aaaaagaaca accagacgag tgctatcacc
     2281 ttgcggcgta ttctttcgtt tcgtattcat ttgatgaaga gttcaccgtt tttaatacga
     2341 acatccatgg cactcataat gtgctttctg cgttaaagaa ctcctcccca ggctgtaaat
     2401 tttattttgc cggctcatcc gaattattcg gtcgcgcaaa aaaatccccc caaaatgaac
     2461 aaacgccatt caacccacgc tcagcctatg gaatcacgaa ggtgacaggt tattacctca
     2521 cagccaatta tcgggagaat tatggcttat acgcctgcac gggcattgcc tataaccacg
     2581 aatcaccccg cagagggttt gaatatgtaa cgcgcaagat ctctcgcggg gtcgcactga
     2641 ttaaagcagg gctgcaggaa aaactggtgc tgggaaacct ggaagcgata cgggactggg
     2701 gttatgcccc tgattacgtg caggcgatgt ggaaaatgct gcagcaggat accccccacg
     2761 atttcgtgat tgcgaccggc attggtcaca cgatacgtga gttttgccaa cttgcgtttg
     2821 agagagtagg gttggattac cgcgattttg tttcttttga tcctaaattc taccgccctg
     2881 tcgaggaagt gccattgatt ggagacgcca cccgtgcagc ggctgacctg aactggtcgc
     2941 cagaggtgag ctttaaagag ctggttcatt tgatggttga tgcggatatt gaacaaatag
     3001 aaagtatttg aagtcccgac cagtattgac tggtaaagac acgatgattt tcgtgagcgc
     3061 aacttctttg gcataaccca cccgattgat ttgggcaaaa agcatatcgg ttataatgat
     3121 tggtcgataa aatggaacaa cctcaaccat ttctgagtta cagtcagtag ttgaatcttg
     3181 ttaggagtaa atcctaatcg caaaggtggc aatggtccgg tctaagaatc aggtcaagat
     3241 ctacgacagt gaaaaggtca agtgggtagc gatcgaagag attaaagccg tttggtttta
     3301 cagagacttg atggttcagc tggtgaagag agatattgtg gcaaggtata agcgttccac
     3361 gctgggtgtg ttgtggacca tgttgaaccc gcttgctacc atgattattc taacaattgt
     3421 tttctctcga ttttttaata tgagaagcgt gtacccggcg tatattatca ccggattgat
     3481 cacctggtct tttttttctc aaaccatctt gttttctctt aataccaccc tgcgaggaag
     3541 cgatctttat aacaagattt atttacctca gaccgctttt gtgatttccg tattaggcgg
     3601 aggaattctc aattaccttt tatcgatcat cccgatggct ctcatttttc tggtgaccaa
     3661 ggtgcccatc catcccaccg ttttgttatt ccctgttgga ttactccttt tagcgacatt
     3721 tactttaggc ttagggctct tgctatcaac cggtgtggta ttcttccctg atattgctga
     3781 atttgcgccg gttatccttc aggcctggtt ctacattaca cccatcatct acccggcaga
     3841 gctgcaattg gacattatgg gcggcctgtt cttgaagatc aacccaatgt attacttcat
     3901 caagctcata cgcaacctgc tttatgatgg cgttatccct tctgggcttg attggttaat
     3961 tgcgttcctg atggcgatat tgatgctcgt tattggctgg tgggttttta ccagtaaatc
     4021 taaacgtttt ggataccagt aatcaaatga ccttaaacga tcctgaggaa cagaccgccg
     4081 tattcctgaa agatgtcagt gtggcatatc gtttgcctgt tgaacgcttc gcttcgatca
     4141 aagaatatgc catcaaaaaa gtgctgggca aagtccatta taaagatttc tttgccttga
     4201 acgatatcaa cctttcgatt aaccggggcg aaatctttgg catcattggt agaaatggag
     4261 cgggcaaatc cacactgctt aaggtgatct caagagtgct ttaccccacg accggtagag
     4321 tttgggtaaa ggggattgtt gcgcctttgc tccagcttgg tgcgggattt caccctgaat
     4381 taaccggtta cgagaacatt tatttaaatg gcactctctt aggacacaaa cgggttgaga
     4441 taaaagacaa gattgatgcg atcttggctt tcagtgagat tggagatttt attaattctc
     4501 caattcgaac ctattcatcc gggatggtgg ctcgtttggg tttctcaatc gccactgcat
     4561 gggtacctga catcctaatc ctggacgagg tgctttcagt gggcgatgct gcattcagag
     4621 aaaaatgcag caaccgaatg caggaattga tcaagagcgg cgcgactgtg ttccttgtga
     4681 cccatgatgc gcaattgatt tccgagttgt gtacaagggc agtgctgctc gaaaatggtc
     4741 atatcgtgtc ttcaggcaca ggagcagagg taagtgtgat ttattccagg tctcttgccg
     4801 atgcgtacag ttgatacagg tgaacttgga tgaaatttac tcctactcgt ttagctgatg
     4861 tgttgctcat cgaaccccgt gtttttggcg atgaacgcgg ttttttcatg gagacctttc
     4921 aacaggaaag gtttaaaaat gcgggtattg accaaaaatt cgtccaggat aaccaatcca
     4981 gatcaattcg cggcacgttg cgcggattgc attaccagat caagcacgcc caggggaaac
     5041 tggtgcgcgt tatatcaggc gaaatcttcg atgtggcggt agacctgcgt cgatcatcgc
     5101 cgacatttgg aaaatgggtg ggatgcaatc tgactgcgga aaacaagcat atgctttgga
     5161 ttccgcctgg attcgggcat gccttctata ccctttctga ttgggctgac gtggtttaca
     5221 aagccacgga cttctattca caacctgatg agcgtaccat catttggaat gacccggaaa
     5281 ttggcattca gtggcctgaa caggtgcagc aaacactgat cctttctgaa aaagatgcgc
     5341 gcggtgtttc atttttgcaa gcggaggttt ttttatgaac ttttcacgcg tgcttgtaac
     5401 aggcggcgcc ggctttatcg gctctaactt catctactat acgctcaagc attttcccga
     5461 cgtgtttata ttgaacctgg acctgctcac ctatgcaggc agcctggaaa acctcaagga
     5521 tattccggct gaagcccgat accagttcgt gcaggcggat atttgcgacc agcaaactgt
     5581 tactgaactc attcgcgcat ataacatcga tgccgtggtg cacttcgccg ctgaatccca
     5641 tgtcgaccgg tcgattctgg gacccgcagc gttcatgaac accaatatct tgggcacctt
     5701 ctctctgctc gaagctgccc gcgctgtatg gctggctgag aaaaaactca aaccagaaca
     5761 ggtgagattc caccatattt ctactgacga ggttttcggt tcactcgagc caggtgcccc
     5821 agcgttcagt gaaaccacgc cctattcacc aaattccccg tacgctgctt ccaaagccag
     5881 cagcgaccac ctggtgcgcg cttaccacca cacgtacaac ctgccggtaa ccctcaccaa
     5941 ctgctccaac aattacggcc cttaccagtt cccggagaag ctgattccct tgatggtgtt
     6001 gaacgcgctt gaaggtaaag cgttgcccat ttatggggat ggcatgcagg ttcgcgactg
     6061 gttgtacgtg gaggatcatt gcgaggcgat ttggaaagtg cttcaagaag gtaaagtagg
     6121 tgaaagttac aatgttggcg gcaacaacca gccgccgaac atcgagatcg ttgatgcgat
     6181 ttgcgcatta atggacgaat ttttacccga ttctgagttc cgcccacacc aaaaactgaa
     6241 aaaatttgta accgaccgtc ccgggcacga ccggcgatac gccatgaaca ccagcaaaat
     6301 tgagcgtgag cttggttgga aaccgcgtca tgatgtggag aaagggctgc gtaaaacggt
     6361 gcagtggtac cttgaaaaca aagcctggat caattccatc agagcgcaaa aacagtatga
     6421 agagtggatg gttaagaact atacaaaaag aggtgaaaaa tgaaagggat catcctggct
     6481 ggcggaaaag gcacgcgctt gtttccactc accctgagca cgagcaaaca attactacca
     6541 gttttcgaca aaccaatgat ctattatcct ctttccatgc tgatgctggc gcgcatccgt
     6601 gagatcctcg tcatcagctc tcctgaggac ctaccatcct ttcgccggct gctgggtgac
     6661 ggttctcaat ggggcctgga gtttgcgtac gctgaacagg ttgaaccccg tgggctggca
     6721 gacgccttca tcgtgggcga aaaatttatc aacggtgaac cctgctgctt gattttaggc
     6781 gataatatct tcttcggtca ggggttacca gagaaactgc gtgagtccgc ggccttaacc
     6841 aggggagcca ttgtgtttgc ctacccggta cgagaccccg agcgttatgg agtggtcgaa
     6901 tttgatgaac agggcaaggc tatcagcctg gaagaaaaac cggccaatcc acggtccaaa
     6961 tttgctgtac cagggttgta tttttatgat gaaaaagtgg tgatgatggc aaaatcgctt
     7021 aaaccttctg cgcgcggtga gattgagatt actgacctga accgcatgta tatggaaaga
     7081 ggggagctca acgtgcacca gatgagccgc ggtactgcct ggttggacgc gggcacgcac
     7141 gagtcattgc tgcaggcttc gaattttatc caggcggtgg aagaacgcca gggaattatg
     7201 atttcctgcc ctgaagaagt cgcctaccgc atggggttta ttgataagga tcagcttctt
     7261 aaattagcga aaatatatga gtccaatagc tatggtaact atctgaagca atttatcgag
     7321 agcgtttcat gaagttttta ttgatcggta agtatgggca actgggatgg gagctcatgc
     7381 gcacctgcat gaccctgggg gagattgttg ccgtggacta tcctgaagtt gacctggcaa
     7441 actcccacgc gttgcgtgag cttgttcaaa cggtcaaacc agatatcctg ttaaaccctg
     7501 cagcatacac cgctgtggaa aaagcggaaa gtgaatttga agtggcgcgt ctggtcaatg
     7561 gggtcgcccc gggcgtgctg gctgaggaga tgaaaaaact tggcggcgcg cttatccatt
     7621 attccacgga ttatgtgttt gacggcgaaa aaggatcttc ttacatcgaa aatgatgcac
     7681 ccaacccgat caacgcctat ggcgtgacca aactggaagg agagcagcag gtggcagccg
     7741 tggaaggcac ttacctgatc ttcagaacca gctgggtgta cagcctgcga cgggcaggat
     7801 ttatcagcaa agtgctcaat tgggcaagaa cgcaagaggt gatgcgcata gttgacgacc
     7861 agattggcag ccccacctgg gccaggatgt tggc
//
LOCUS       DAOTLW010000058         7748 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_9248_length_7748_cov_9.148577, whole genome shotgun sequence.
ACCESSION   DAOTLW010000058 DAOTLW010000000
VERSION     DAOTLW010000058.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9248_length_7748_cov_9.148577"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            195..1028
                     /locus_tag="P5516_07880"
     CDS             195..1028
                     /locus_tag="P5516_07880"
                     /inference="COORDINATES: protein motif:HMM:NF023568.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HRT92389.1"
                     /translation="MKHSEGTFQGVRDLKIYHQAWLPEGKPRAAILIVHGLGEHSGRY
                     ANVVNRFVPSGFALYGIDHIGHGKSGGEREMLARFEDFTEPLAVYQKMIAEAHPGLPL
                     FIYGHSMGGLITAFYLLDHQKEFTGAILSAPAVKIPQNISALTVTLGRVFSTIAPKMG
                     LIQLDASGISRDDAVVKAYNNDPLVFHGKTTARLSAEMLRAMMRVTAEVEKITLPLFI
                     LQGSADYLVDPDAAKMVYEKAASVDKTLKLYDGLFHEVHNEPEREVMFNDLETWINAH
                     I"
     gene            1288..1632
                     /locus_tag="P5516_07885"
     CDS             1288..1632
                     /locus_tag="P5516_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HRT92390.1"
                     /translation="MSHNSHLYIVWTTDNPITAMNMVTTYGGNSMKNGWWDEVTIILW
                     GASQQLVATDPSVAGRVRELMEMGVKFSACKRCADELRVTDALERLGVEVIYWGEPMT
                     RLLKSGATVLTI"
     gene            1923..2354
                     /locus_tag="P5516_07890"
     CDS             1923..2354
                     /locus_tag="P5516_07890"
                     /inference="COORDINATES: protein motif:HMM:NF013629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HRT92391.1"
                     /translation="MMNSDQAATLLKQKGVRPSYQRLRVLQYLHEHIGHPTAEEIFTA
                     LSVDIPTLSRTTIYNTLHTFVSYGLVNCLTLDGVETRYDVVLQKHGHFKCHRCGTIFN
                     FMMDVDRIETSGLEGFVIENKNIIFTGICPDCSFKKTKKGE"
     gene            2358..3827
                     /locus_tag="P5516_07895"
     CDS             2358..3827
                     /locus_tag="P5516_07895"
                     /EC_number="1.11.1.6"
                     /inference="COORDINATES: protein motif:HMM:NF012425.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="catalase"
                     /protein_id="HRT92392.1"
                     /translation="MSDKKKLTKLTTAAGAPVPENDNALTAGPRGPMLLQDVWYLEKL
                     AHFDREVIPERRMHAKGSGAFGKFTVTHDITKYTRAKIFSKVGKETECFVRFSTVAGE
                     RGAADAERDIRGFAIKFYTEEGNWDLVGNNTPVFFIRDPHKFPDLNRAVKRDPQTNLR
                     SATNNWDYWTSQPEALHQITITMSERGIPYSYRHMHGFGSHTFSMINDKNQRVWVKFH
                     LVTQQGIKNLTDAEAEAVIAKDRESHQRDLFDSIARGDYPRWKMFIQVMTEEQAKKMP
                     YNPFDLTKVWFKKEFPLIEVGVLELNRNPENYYQDVEQAAFNPAAVVPGIGFSPDKML
                     QGRLFSYGDAQRYRLGVNHHQIPVNNPKNAPAHASHRDGLMRVDGNHGATLGYTPNSF
                     GEWEAQPQYKEPPLELSGAADHWDARADDSDYYTQPGKLFRLMNAAQQKVLFENTARA
                     MGDAPEFIKIRHIGNCLKADPEYGKGVAKALGISMEKVK"
     gene            complement(4026..4628)
                     /locus_tag="P5516_07900"
     CDS             complement(4026..4628)
                     /locus_tag="P5516_07900"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRT92393.1"
                     /translation="MNAFLHITAEAAGFNWYAWVVLPLIIFVARIFDVTLGTLRIIFV
                     ARGKRNLAPILGFFEVLIWIVVISQLVQNLESATAYIGYAAGFAAGNYFGMWLEDKLA
                     LGTYILRLITAEEPGELVRRLRAAGFGVTTVAGEGKTGTVQLIYTFVKRRDVEAVLSI
                     IHELKPNAFVSMEEVRSTEKGIFPGGMAAQARGSLLRKSK"
     gene            4784..5485
                     /locus_tag="P5516_07905"
     CDS             4784..5485
                     /locus_tag="P5516_07905"
                     /inference="COORDINATES: protein motif:HMM:NF019500.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HRT92394.1"
                     /translation="MTAQQQFASIRKRLLDFHPSAFLLAAQLLQLILYAAFEGMISQR
                     SLINAAGLVVLLMVLWVVTSSARVQWVAWLIAAPALGLSLLSAIFATPALQAWSSLLE
                     AILYFFTAGSLIVYMMGDNYVTTDELFAVGATFTLLAWGFAHAYLVCQIWAPGSILIS
                     TAPGREATFLELLFLSFTNLSATGLGDIITVSAHTRVLAMLEQFLGVGYVAMVVSRLI
                     GLVNNTRKRHKSEER"
     gene            5570..5998
                     /locus_tag="P5516_07910"
     CDS             5570..5998
                     /locus_tag="P5516_07910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92395.1"
                     /translation="MKNALKEVDRIIYTVDSGPILPELQAHEVYTITPSNITLKRTGI
                     YSNTEVYEGEWAFSGDEALLQELFTIAKRKDCSAYSRVEPEDAPDGGHTIRLTVVYAD
                     ESECTLIYDPGVTYKGADELLDKLKEVLNNLKSHPSMAPK"
     gene            complement(6194..7390)
                     /locus_tag="P5516_07915"
     CDS             complement(6194..7390)
                     /locus_tag="P5516_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92396.1"
                     /translation="MKLKKMVLIFSAAALILSLSSCNLPVANSGESSAEKTLMAIYVA
                     QTIAAANAAQPVQVETPAVAEPSITEVPSQVSTPEPTSTKEIIHNLFPGEPGFISQYW
                     MDTNTSSVASEKRAYGGDNFSNNFFERPFTSIEMLYRPDIDLVRVEISSDANFYYFNL
                     HLYGKSPEQDTLAGNYGVEIDFNRDGRGDLLIWTTADGNTAWNIDKIFVFRDSNNDVG
                     GQRPMRSDAPGYSGNGYDLLVFSPDHLDDPDAAWKRVSPQNPNVLQLAVKKSLFENRA
                     VFMWNGWADAGVKDPARFDYNDAFTAAEAGSPYAGNVDYPLKSLFLVDNTCRLAYGFT
                     PTGLEVGACLIYTPTPTPSATPTQPVCDCSSFRNYSYIDTKECCEYCGYRWAGTSEFP
                     CYAPRP"
BASE COUNT         1968 a         1974 c         2034 g         1772 t
ORIGIN      
        1 gaattaatga ttttcgttta ttgcaataat cgcaagagcg ggttgcgccc gggtttttca
       61 tttgaaacgc caaagagcgc tcgcctgtag ccacgagcag caagtggacg cgcttgcggg
      121 tgaaaattca atttgcaaac ctgtgatata gtggaagagt cagttcgtta gttcatcaac
      181 aaagggagag aaaaatgaag catagtgaag gcactttcca gggtgtgcgt gatttaaaga
      241 tataccatca ggcatggttg cctgagggaa agcccagggc ggcgatactt attgtgcatg
      301 gattgggaga acacagcggt cgttatgcca acgtggtaaa ccgtttcgtg ccatctggtt
      361 tcgcgctcta cggcatcgat catattggtc acggaaaatc cggcggagag cgggaaatgc
      421 ttgcgcggtt tgaagacttt accgaaccgt tggcggtata tcagaaaatg attgccgaag
      481 cccatccggg tttaccgctt tttatttacg gacacagcat gggcgggttg atcaccgctt
      541 tttacttgct tgaccaccaa aaagaattta caggagcgat cctctccgcg cccgccgtta
      601 aaatccctca gaatatttct gcattgacgg tgaccctggg cagggtattt tcgacgatcg
      661 ctcccaaaat gggattgatt caactggacg cttccggcat ctcgcgagat gacgcggttg
      721 tgaaagccta caacaatgac cccctcgttt ttcatgggaa aactaccgcg cggctttctg
      781 cggagatgct gcgcgccatg atgcgggtca cggctgaagt agagaaaatc accctgccat
      841 tgtttatttt gcagggtagc gctgattatc tggtcgaccc ggacgcggcg aagatggttt
      901 acgagaaggc tgcttcagtc gacaaaacgc tcaagttata tgatgggctg ttccacgaag
      961 ttcacaacga acccgagcgc gaggtaatgt tcaacgacct cgaaacctgg ataaacgcgc
     1021 atatctgatc aataaattaa aaaaaaccac cggcagaaga gggcactctg ccgggttgat
     1081 ttaatggatg aatggattgg cacacacaac gttacggctt accagcaggg agatcacccg
     1141 ctgtagggct ggttcaccgc cgctctcagt gacgaggatg aaaagcgcgt tggtataatc
     1201 gagccatctg cgcgccagcg aacagcaccg ctcacgagca taaggatagt gacgaaggcg
     1261 caaccaggtt tgaccaagga gaggtaaatg agccacaact cgcatctgta cattgtctgg
     1321 acaacagaca acccaattac cgccatgaac atggtgacca cgtatggcgg taacagcatg
     1381 aagaatggct ggtgggatga agtgaccatc atcttgtggg gagcatctca gcaattggtc
     1441 gccacggatc ccagcgtggc aggacgcgtg cgcgagttga tggaaatggg ggtcaagttc
     1501 tccgcttgca aacgctgcgc ggatgaactg cgggtgactg acgcgctcga gcgcctgggc
     1561 gtagaggtga tctattgggg ggaaccgatg acgcgcttgc tgaaatccgg cgcgactgtg
     1621 ctgacgattt aaaggtctac agggcagggg gcaaggcacc ctattcttca ccatcaagtt
     1681 gaaagaaaga gaaccgcggg cttcgcgtat ggccgaaatc cggtatgtgg agcattctaa
     1741 tacgcttgtg acacgcgaaa gatggcggaa aatggatgag agtccatcgt gattcgctaa
     1801 gaggcaacac ccgcttcgcg ctgcgccaac ctatcctgtt ggtgttttgg catcctttaa
     1861 tttatattga aacaaaacct atattgtaat cattacaata atgatgtata atcaactaac
     1921 agatgatgaa ttcagatcaa gctgctacac tgcttaaaca gaagggcgta agaccctcct
     1981 accagcgctt gcgggtgctg cagtatttac acgagcatat tggtcaccct accgctgaag
     2041 aaatattcac cgcgctttca gtcgatatcc ccacactttc acgcaccacc atttacaata
     2101 ccctgcatac cttcgtcagt tacggcctgg tgaactgcct gacactggat ggggtggaaa
     2161 cccgttatga cgtggtactg caaaaacacg ggcattttaa atgtcaccgc tgcggaacca
     2221 tttttaattt catgatggac gtggaccgga tcgaaacgtc aggtctcgaa ggattcgtaa
     2281 ttgaaaataa aaacatcatc tttacaggta tttgtccgga ttgttcattt aaaaaaacca
     2341 aaaaaggaga ataacagatg tcagacaaga aaaaattaac caagctcacc accgcagccg
     2401 gcgcgccagt gccggaaaat gacaacgcct taaccgccgg tccgcgcggc cccatgttgc
     2461 tgcaagacgt gtggtatttg gaaaaactgg cccattttga ccgtgaggtc atcccagaac
     2521 ggcgtatgca cgccaaaggc tcaggggcgt ttggcaagtt caccgtgacc cacgacatca
     2581 ccaaatacac ccgcgcgaaa atcttctcta aggttgggaa agaaaccgag tgttttgtgc
     2641 gtttctcaac cgttgccgga gagcgcggcg cggcggatgc cgaacgtgat attcgtgggt
     2701 ttgccatcaa gttttacacc gaagaaggta actgggacct ggtggggaac aatacccccg
     2761 tcttcttcat ccgcgatccg cacaagttcc ctgacctcaa tcgcgctgtc aaacgcgacc
     2821 cgcaaaccaa cctgcgctca gccaccaata actgggatta ctggacaagc cagccggaag
     2881 cgctgcacca gatcaccatc accatgagcg aacggggcat cccatactct taccgccaca
     2941 tgcatggttt cggcagccat actttcagca tgatcaacga caagaatcaa cgtgtatggg
     3001 tgaaattcca cctggtcacg caacagggca ttaaaaacct cactgacgca gaagcggaag
     3061 cggtcatcgc taaagaccgt gaaagccatc agcgcgacct gttcgacagc atcgccaggg
     3121 gagattaccc ccgctggaag atgttcatcc aggtgatgac cgaagagcag gcgaaaaaga
     3181 tgccctataa cccctttgac ctcaccaaag tttggttcaa gaaagaattt ccgcttatcg
     3241 aggtaggtgt ccttgagctg aatcgcaacc cagagaacta ttaccaggat gtagagcagg
     3301 cggctttcaa cccggcagcg gttgtccccg gcatcggttt ttctcccgat aagatgctgc
     3361 aaggtcgatt gttctcctac ggagacgctc agcgttaccg actgggggtt aaccatcacc
     3421 agatcccggt gaacaacccc aagaacgccc cggcacacgc ttctcatcgt gacggtttga
     3481 tgcgcgtgga tggcaaccat ggcgccacat tgggttatac ccccaacagc ttcggtgaat
     3541 gggaagccca accgcagtac aaggaacccc ctctcgaatt gagcggcgcc gctgaccatt
     3601 gggacgcccg cgcggatgac tcagattact atacccaacc aggcaaactc ttcaggctga
     3661 tgaacgccgc acagcagaag gttctctttg agaacactgc ccgcgccatg ggtgacgcgc
     3721 ccgagttcat caagatccgc cacattggca actgccttaa agcagacccg gagtacggca
     3781 agggtgtggc caaggctttg gggatctcca tggaaaaagt taagtaaaaa gacctgtacc
     3841 gccttcgggg cagccgtttg gctgccccgg ttttttaaaa cctggttgct ttattccgtg
     3901 atcgggagac agatttaaga taaagacagg gcaagatatc caggttgacc ttgctgagag
     3961 gtaacccttc cgccggctca cccgggagag tcactctgcc tgagccttga agagtgccga
     4021 gggacttatt tcgattttcg cagtaatgac ccgcgagcct gagcggccat accgccaggg
     4081 aagatgcctt tttctgtgga gcgcacttcc tccattgaaa cgaaggcgtt gggttttaat
     4141 tcatggataa tggaaagcac cgcttccaca tcgcggcgct tgacaaaagt ataaatcagc
     4201 tgaaccgtgc ccgttttccc ttcgcctgca accgtggtca ccccgaaccc cgcggcgcgc
     4261 aggcgccgaa ccagctcccc cggttcttca gcggtgataa gccgcaggat gtaggtgccg
     4321 agcgccagtt tgtcctccag ccacatgcca aaataattac cggcggcgaa acctgcagcg
     4381 taaccgatat aggctgtggc gctttcaagg ttttgcacta actggctgat gaccacaatc
     4441 cagatgagca cctcaaaaaa accgaggatg ggcgcaaggt ttcgcttacc gcgcgccacg
     4501 aagatgatgc gcagcgtgcc cagggtgaca tcaaaaatcc tggcgacgaa gatgatcagc
     4561 ggcagcacca cccaggcgta ccaattaaaa cccgcggctt cagcagtgat gtgtaagaac
     4621 gcattcataa aaatacctcc acaggtacat ctgttcgtga atcaaagcct gctgcgagta
     4681 taacaaagaa agctttcagg tgcatccatt attgagatgg tactgagctc acaaagattt
     4741 ttctctctac catcaataaa attccactat aattgagcca atcatgaccg ctcagcaaca
     4801 attcgcatcc attcgaaaac ggctgctcga ttttcaccca tccgcctttc ttttagccgc
     4861 ccaattactt caattgatct tatacgcggc ctttgaaggc atgataagcc agcgctcatt
     4921 gatcaacgcg gcagggttgg tggtgttgtt gatggtgtta tgggtggtca ccagcagcgc
     4981 gcgggtacag tgggtagcgt ggctgattgc cgctccagcc ctgggtcttt cactgctctc
     5041 agcgatcttc gctacgccgg ctttgcaggc ctggtcttcg ctgctggaag cgatcttgta
     5101 tttctttaca gcaggcagcc ttattgtata catgatgggt gacaattacg taaccaccga
     5161 tgagcttttc gctgttgggg caactttcac actgctggcg tggggcttcg cgcatgctta
     5221 cctggtgtgc cagatatggg caccgggcag catcctcatc agcaccgcgc caggaagaga
     5281 agcgacattt ttggaactgc tcttcttaag ttttacgaac ttatccgcca ccgggctggg
     5341 cgatataatc accgtgtctg cgcacacacg ggtattggcg atgctggagc aattcttagg
     5401 ggtgggctat gtagccatgg tagtttcgcg tttgatcggt ctggtgaaca acacgcgcaa
     5461 acgtcacaaa tcggaggaac gctgaaaggt atccatggtc attcggctga ataaattcac
     5521 ggtctgtttc gcatgcttga tctttttatt ttttttcacg gggtgtgagg tgaaaaacgc
     5581 gttaaaggaa gtcgaccgca tcatttacac ggtggattcg gggcccatcc tgccggaatt
     5641 acaggctcac gaggtttaca ccatcactcc cagcaacatc actctgaaac gcacaggtat
     5701 ttattcgaac acagaggtgt atgagggaga atgggccttc tcaggggatg aagcgctttt
     5761 gcaagaattg ttcacgatcg caaagcgcaa ggattgctct gcgtatagcc gcgtggaacc
     5821 cgaagatgcg cccgatggcg ggcataccat caggttgaca gtggtctatg cagatgaaag
     5881 tgaatgcacg ttgatttatg accctggcgt tacttataaa ggcgcggatg aattactcga
     5941 caagctcaag gaagtgttga acaacttaaa aagccatccg agcatggcac ccaaatagat
     6001 agcgggagga ttttcatcgc taaaaatcct cccgcgtttt gccgattcgc cctgaacaag
     6061 atcagggcta tgccgtttgc tgaggattat ttcatgctct ctgcctgtgt gagcgtgcag
     6121 taagcccttt gggttcgagg ggacaccttt cgctcatttc cgagcgggac gcccaactga
     6181 acaagctgat attctaaggt ctaggtgcgt agcagggaaa ttccgaagtg cctgcccagc
     6241 gatacccaca gtactcgcag cattccttgg tatcgatata actatagttg cggaaagaac
     6301 tacagtcaca aacgggttgt gttggcgtgg cggagggtgt aggtgtgggt gtatatatca
     6361 ggcaagcgcc aacctccagg ccggtgggcg taaaaccata cgccagccgg caggtattat
     6421 cgaccaggaa gagacttttc agggggtaat ctacatttcc agcgtaagga gaacccgcct
     6481 ctgcggctgt gaaggcgtca ttgtaatcaa acctggcggg gtccttaacc ccagcatcag
     6541 cccagccgtt ccacatgaag accgcccggt tttcaaaaag cgattttttg accgccagct
     6601 gcaacacatt ggggttttgc gggcttaccc gtttccaggc ggcgtccggg tcatcaaggt
     6661 gatcaggcga aaagaccagc aggtcgtaac cgttccctga ataacccggg gcgtcagagc
     6721 gcatcgggcg ttgacccccc acatcattgt tgctatcccg gaaaacaaaa atcttatcaa
     6781 tattccaggc agtgttgccg tccgcggttg tccatataag caggtccccg cgaccgtccc
     6841 ggttaaaatc tatttcaacc ccatagttgc ctgccagcgt gtcttgctcg ggtgatttac
     6901 cgtaaaggtg caggttgaaa tagtagaagt tggcgtcaga actgatctcc accctcacca
     6961 aatcaatatc ggggcggtac agcatttcga tggaagtgaa cggcctttcg aaaaagttat
     7021 tggaaaaatt atctccgcca taagcgcgct tttcgctggc aaccgaagag gtattggtgt
     7081 ccatccagta ctgggaaata aaccctggct ccccggggaa caggttgtgg atgatctctt
     7141 tagtggatgt gggttcgggg gttgacactt ggctgggaac ctcggtgatg gatggctctg
     7201 ctaccgcagg tgtttccacc tggacgggtt gagcagcgtt ggcagcggca atcgtctgag
     7261 caacgtagat ggccatgagg gtcttttcgg cgctactttc accagaattg gccaccggga
     7321 ggttgcacga tgacagcgac aaaatcaaag ccgcggctga aaaaattaat accatctttt
     7381 tgagtttcat tttttcctca ctttcgcgac ctccctgatg gagacggtca actggctcag
     7441 tcggtttttt tattatagca aaaaaacact tcaacgagcc tgcgcgcacc cctctcactt
     7501 gcgccatgcc gaatttatga cggccaagga aagcaacccg cacaaccgtt cacgattaac
     7561 acgtggcact atttacggtt caaaaagaca gggggaaaac cctaccgaga aagggccttt
     7621 gctggcacat cctccatggg tgggtcaggg tgcctttaca ttcaccctgt gacaaacctg
     7681 gcaaacagcg tccctattga ataattcagg tgtttttact agaataacag taaaggaggt
     7741 tatgatgg
//
LOCUS       DAOTLW010000059         7412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_9706_length_7412_cov_9.643333, whole genome shotgun sequence.
ACCESSION   DAOTLW010000059 DAOTLW010000000
VERSION     DAOTLW010000059.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7412
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9706_length_7412_cov_9.643333"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="P5516_07920"
     CDS             <1..410
                     /locus_tag="P5516_07920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92397.1"
                     /translation="WTARVLIAAVTFMNLQAAIQFLLEPRAYAWGFELSGVPGEAMVQ
                     GLGLLFLMWSVPYLFAVVDPQKHFFSLVTAVIMQFIGFAGESLLLTGLEEGHPLIVSS
                     VTRFIIFDGAGLVALLLALWLVVFDRKASIKKA"
     gene            complement(444..1235)
                     /locus_tag="P5516_07925"
     CDS             complement(444..1235)
                     /locus_tag="P5516_07925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HRT92398.1"
                     /translation="MRNGFLILLIFFAFFTPFRSTLLIIGIDRAPEEGSVLGRSDTMI
                     LTTLPPVLPKMSLISIPRDLWVSIPGYGENRINTAHYFAELYQPGTGMQAAAGVIEAN
                     FGIAVPYVIRLRFEGFRNIVDAMGGVTIHLSEPMSGLDAGTHTLDGAQALAFVRDRAG
                     SDDFWRQKRGQMFISAAIQNMLNPVKWPRIPAVITATLQAVDSTIPFYAWPRLLYGAA
                     FSAVFGFESATFDRNWVTPWVTDEGAQVLLPNWEFITPFVKEVFK"
     gene            complement(1415..1915)
                     /locus_tag="P5516_07930"
     CDS             complement(1415..1915)
                     /locus_tag="P5516_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HRT92399.1"
                     /translation="MSEEPRKIAFICSKGNLDMVYPALVMGWAALGNGIDVTIFFTFW
                     GLDMINKARVDHLEIPPIANTSMKMNMMGVPGYIGIPQIMGIIPGMTAFASALMKKKM
                     KGLEVPTVREYLEMLVDAGANLYACKMSVDMFDLKKEDFVDGVKDIVTAGDFMDMTEG
                     AQIVFI"
     gene            complement(1944..2258)
                     /locus_tag="P5516_07935"
     CDS             complement(1944..2258)
                     /locus_tag="P5516_07935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HRT92400.1"
                     /translation="MATKKIAGVDVQVNEEGFLTNPAEWNKEIAVELAKEEGIELTDA
                     HWKVIDFCRSAAAVNGAAPTLRSITNGSGVSTKDLFALFPKGPAKKVAKISGLGKPEG
                     CV"
     gene            complement(2318..3559)
                     /locus_tag="P5516_07940"
     CDS             complement(2318..3559)
                     /locus_tag="P5516_07940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding oxidoreductase"
                     /protein_id="HRT92401.1"
                     /translation="MKTFLILGAGTGGTMMANKLVKRLDPKEWKIILVDKDETHYYQP
                     GFLFLPFEYYKPEEVYQPKKKFVPSGVEFILSDVELIEPEANKVTLAKDKKVIHYDQL
                     LIATGTDIHPEEVDGMLDGWRQNIFDYFTFDGAMALREFLKNFKGGRVVLNIKEMPIK
                     CPVAPLEFVYLADYFFTQKGMRNKVDLIFTTPLSGPFTKPVSNKLLGDVMTKKGIHTE
                     AEFDVMEVDAAKNIIRGYDGRELEYDLLVSIPTNMGSDVIKRSGMGDDLYFVPTNKDT
                     LRSEKYENIWVIGDAANIPASKAGSVVHYAAETLIENILDALEGRPLSHKYDGHSTCH
                     ILSGFNKSLLIDFSYDTEPLTGLYPFPVIGPFPLLKESWFNYIGKLSFKWIYWNIMLR
                     GIPFPLPPEYSIAGKKQVAVS"
     gene            complement(3579..3926)
                     /locus_tag="P5516_07945"
     CDS             complement(3579..3926)
                     /locus_tag="P5516_07945"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HRT92402.1"
                     /translation="MSDNLREEVVRLHAQVCSGLADPNRILIIYNLAEGPRCVNEIAA
                     SLELSQPTVSRHLKVLRERGIVRAERDGQTIHYRLSDARIIHALDILRSILADQLKDQ
                     VLLARQVNEKITP"
     gene            complement(4083..5987)
                     /locus_tag="P5516_07950"
     CDS             complement(4083..5987)
                     /locus_tag="P5516_07950"
                     /inference="COORDINATES: protein motif:HMM:NF014466.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005509 - calcium ion binding [Evidence
                     IEA];
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thrombospondin type 3 repeat-containing protein"
                     /protein_id="HRT92403.1"
                     /translation="MTTLQRKKTIIKRILWILLGMLLLALLFVGVLFFIHVLRVTSSP
                     SIDILSPVKGQTAPSLTPFTIQAMAKQRRVPIRRLDLYLDGFLYGSVSGESDSLLGRW
                     NWTPVLTGDHELAFIATNTRGVMNIVQQKIEVTPVADTDHDGIPDSIDVCPEEYGYSE
                     SHGCLHPADIDGDGLIGDDDACPEVFGFAIDGGCPPDARPDTDGDGILDRADLCPEES
                     GLAELAGCPVESWFVDRDGDAIPDFLDRCPDEAGTGEALGCPHVTEGDADGDGIADAE
                     DDCPDEPGGRSTRGCPLEVSEDSDGDGVADSVDRCPEEAGVGSGDGCPIDDWLVDTDL
                     DFIPDLFDLAPDLPGLFEWLGLPLAGDTDSDGVPDSEDRCPELAGSPADCGCPRFTLP
                     LADIARQNLFSPFAFAGGAEPGGVGAGSKEAAYPYDIDRDGVHDRWDDECVGEHGEPA
                     QHGCAPVDDDDRDGIPNHIDRCDTIPGLYWGEYTDTYMFGCPREPAGEVNLELSITAA
                     RTPTEMQAIYCFAHPFPKSPVGLVNRIPPPYRNYSVDFLERYGYYLRLRDFRLNLSIP
                     NVKETEVITLYIACWGQPKGISLPARFLGEIYREVPVQDWDSQVRYAGGVGDSTMMEV
                     FYRVCRNHCP"
     gene            complement(5998..7080)
                     /locus_tag="P5516_07955"
     CDS             complement(5998..7080)
                     /locus_tag="P5516_07955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92404.1"
                     /translation="MKICRFLIPLMGLLLLAGCGLHTGGIGPGHAIWVDGPVEKETYV
                     NTSVSITVHSNYELSEVFIQYNLLSSSAVPLATVPMHQVGSTIFEGVTVWVPTLPGEY
                     NLRAYSADSVVSGARYLKVLPQPTPSSHSTPTAKSLPMTADTAIPTSAPPTILPDIQF
                     SADAYTLTAGECTFLRWSTVFVESAYLNGERVELMHSRQVCPVASSTYTLRGESRVGS
                     IEKSLTIYVSPPEVPPPSPPDTQGPSITEITKSAESIFDNTSCGLASNTISASVTDVS
                     GVSEVVLWFRAKNSSSATPGEWRSLTMSNTGGTTYQAALGISQLASSLSFYADGIVEF
                     YIIAKDSAGNFTQSGVRTFTTKMCLI"
     gene            complement(7316..7389)
                     /locus_tag="P5516_07960"
     tRNA            complement(7316..7389)
                     /locus_tag="P5516_07960"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(7353..7355),aa:Val,seq:cac)
BASE COUNT         1686 a         1884 c         2093 g         1749 t
ORIGIN      
        1 cctggaccgc ccgggtgttg attgcggcgg tgacttttat gaatcttcag gcggccatcc
       61 agttcttgct ggaaccgagg gcttacgcct ggggctttga gttgagcggc gttccaggcg
      121 aagcgatggt tcaggggctg ggtttgcttt ttttgatgtg gagcgtcccc tatctcttcg
      181 cggtggtgga cccgcagaag catttcttct cacttgtcac ggctgtaatc atgcagttca
      241 ttgggtttgc aggtgagtct ctgctcctga ccggattgga agagggacac cctctgatcg
      301 tttccagcgt aacgcgattt atcattttcg atggcgctgg gttggtagcg ctgttattgg
      361 ctttgtggct ggttgtcttt gacagaaaag cgtcaataaa aaaagcctga cgggcaggct
      421 ttttacaggt taaggttcag cgattattta aacacttctt tgacaaaggg ggtgatgaac
      481 tcccagttgg gcagtagcac ctgcgcgcct tcgtcagtca cccagggggt cacccagttg
      541 cggtcaaaag tggctgactc aaagccgaaa acagctgaaa acgcggcgcc gtaaagcagg
      601 cgtggccagg cgtaaaaggg aatggtgctg tcaaccgcct ggagcgtggc ggtgataact
      661 gccgggatgc gtggccattt gaccgggttg agcatattct ggatggcggc ggagatgaac
      721 atctgtccgc gcttctggcg ccaaaaatcg tcgctgccgg cgcggtcgcg cacgaaagcc
      781 aacgcctggg cgccatccag ggtgtgggtg ccggcatcca acccagacat tggctcggac
      841 aggtggatgg tcacccctcc catcgcgtcg acgatgttcc tgaagccctc gaaacgcagg
      901 cggataacgt aagggacagc gatgccaaaa ttcgcttcga ttacaccggc agccgcttgc
      961 atgccagttc cgggctggta aagctcggcg aaatagtgcg ccgtgttaat gcggttctcg
     1021 ccgtacccgg ggatgctgac ccacaggtca cgcgggatgg agatcaggct cattttgggc
     1081 agaactgggg gcagggtggt gaggatcatg gtgtcactgc gccccagaac gctgccttct
     1141 tctggcgcgc ggtcgatgcc gataatcagc agcgttgacc ggaaaggcgt aaaaaaggca
     1201 aaaaagatga gcaggataag aaagccgttg cgcacagggt gcctgcgacg cctttggcgc
     1261 tgcggtactc ctgctcctgc atgggggtgt gttggagcgt actggtaata ttgattcatt
     1321 gtgtcatttt aacctgtctg cctggatctg tcaggcaggc aaaaaaagcc ggagtggaga
     1381 ccaccccggc ggaatgcgca taacaaaagg tggattagat gaagacgatt tgagcgcctt
     1441 cagtcatgtc cataaaatca cccgcggtga cgatgtcttt gacgccatca acaaaatctt
     1501 ccttcttcaa gtcgaacatg tccaccgaca ttttacaggc gtagaggttc gcgccggcgt
     1561 ccacgagcat ttcgaggtat tcgcgcacgg tgggcacttc caatcccttc attttctttt
     1621 tcatcagggc actggcgaaa gcggtcatac cggggatgat tcccataatt tgcgggatgc
     1681 cgatgtaacc aggcacgccc atcatgttca tcttcatact ggtattggcg ataggcggga
     1741 tttccaggtg gtcaacgcgc gccttgttga tcatatccag accccagaag gtaaagaaaa
     1801 tggtcacatc gatgccgttg ccgagcgctg cccagcccat caccagggcg ggatatacca
     1861 tatccaggtt gcctttggag caaataaatg cgatcttgcg gggttcttct gacatttttt
     1921 cctctctctt ttagatgatg agattagaca caaccttcag gtttgcctaa ccctgagatt
     1981 tttgccactt tcttggctgg ccctttgggg aaaagcgcga aaaggtcttt ggtgctcaca
     2041 ccagagccat tggtgatgga gcgtaaggtg ggagcagcac cattgaccgc tgctgcgctg
     2101 cggcagaaat cgatgacctt ccagtgcgcg tcggtcaact cgatgccttc ttctttagcg
     2161 agctcgacag cgatttcttt gttccattct gccgggttgg tcagaaagcc ctcttcgttc
     2221 acctgcacgt caacccctgc gatcttcttt gttgccatgt ttgttcctcc ttagaaaaat
     2281 tcagttaatt tgtttcaaga atttcaggcg cgggggttca agataccgcg acctgttttt
     2341 taccagcgat ggaatattca ggcggcagcg ggaaggggat accgcgcagc atgatattcc
     2401 agtagatcca cttgaatgag agtttgccga tgtagttgaa ccagctttct ttcagcaagg
     2461 ggaaaggccc aatgacaggg aagggataca ggcccgtgag cggttcagta tcataactaa
     2521 aatcaatgag caggctcttg ttgaacccag aaaggatatg acaggtggaa tgaccgtcgt
     2581 acttgtggga gaggggcctg ccttcgaggg cgtcgagaat attctcaatg agggtttcgg
     2641 cggcgtagtg cactactgaa cctgcttttg aagccgggat attcgctgca tcgccgatga
     2701 cccaaatatt ttcatacttt tctgagcgca gggtgtcttt atttgtgggc acaaagtaca
     2761 ggtcgtcacc cataccggag cgtttgatca catctgaccc catgttcgtg gggatggaaa
     2821 ccaacaggtc atattcgagc tcgcgcccat catagccgcg gatgatattt tttgccgcgt
     2881 cgacttccat tacatcaaac tccgcttcgg tgtgaatgcc ttttttggtc atcacatctc
     2941 ccagcagctt attggacacg ggtttggtaa atggccctga aagcggcgta gtgaagatga
     3001 gatcgacctt attgcgcatg cccttttgtg taaagaaata atccgccagg taaacgaatt
     3061 ctaatggcgc caccgggcat ttgatgggca tttccttgat gttgagcaca acgcgcccgc
     3121 ccttgaaatt cttaaggaac tctctgagcg ccatggcgcc atcgaaggtg aaataatcga
     3181 agatgttctg cctccagcca tccagcatgc cgtctacttc ttcggggtga atgtcggtgc
     3241 cggtggcgat gagcagctga tcgtaatgga tgaccttttt atctttggcc agggtcacct
     3301 tgttggcttc aggctcgatc aattccacat ctgaaaggat aaattcaacg ccggaaggga
     3361 cgaacttctt cttgggttgg tacacctctt cgggtttgta gtattcaaag ggtaaaaaca
     3421 gaaaaccagg ctggtaataa tgagtctcgt ccttgtcgac caggatgatc ttccattctt
     3481 ttggatcaag gcgctttacc agcttgttcg ccatcatggt tccgccggta ccggcgccga
     3541 gaatcagaaa tgtcttcatg cattttctcc gtagtggttt agggtgtgat tttttcgttg
     3601 acctgccgtg ctaagagtac ctggtctttc aactgatcgg ccaggatgga acgtaagata
     3661 tccagagcgt ggatgatgcg cgcatcactg agacggtaat ggatggtctg tccatctcgt
     3721 tcggcacgca cgatgccgcg ttcgcgcaac actttgaggt ggcgtgaaac ggtgggttgg
     3781 gaaagctcga gtgaggcggc gatctcgttt acgcaccgcg gaccttcggc gaggttgtaa
     3841 ataattaaaa tgcggttagg gtccgccagt ccactgcaga cctgggcgtg cagccttacc
     3901 acttcctcgc gcagattatc gctcattgtt tatccctgta aattaccaat atatacatat
     3961 acgtttatgt gaatatatta tacgacacat ataaaaatcg catatgtgac atttatcacc
     4021 atttgggcgg agaataagaa actgccgctg ttcaaggcgg cagttggagg gttgcgacca
     4081 ggctatgggc aatggttgcg gcatacccgg taaaacactt ccatcatggt gctatcgcca
     4141 acgccgccgg cataccgcac ctgagagtcc cagtcttgga ccggaacttc gcggtagatc
     4201 tcacccagga acctggcggg aagcgaaatc cctttaggtt gcccccagca tgcgatgtaa
     4261 agcgtaatca cttctgtttc tttcacattt gggatggaca aattcagccg aaaatctctc
     4321 aggcgcaggt aataaccata cctttcaagg aagtcgactg aatagtttct gtatgggggt
     4381 ggaatgcggt ttaccagccc tacaggcgat tttggaaagg gatgggcaaa acaataaata
     4441 gcctgcattt ctgtaggggt cctggctgcc gttatggata gctcgagatt aacctccccg
     4501 gcaggctctc ttggacagcc gaacatatat gtatctgtgt attctcccca atacagacct
     4561 ggaatggtat cgcagcgatc gatatggttg ggtatgccat cacggtcgtc gtcatcaact
     4621 ggagcgcagc cgtgctgagc gggctcacca tgttcgccca cgcattcatc gtcccatcga
     4681 tcatggacac catcacgatc aatgtcatac ggataggcgg cttccttcga gccggcacca
     4741 acacctccgg gttcagcacc ccctgcgaaa gcaaaaggag aaaaaagatt ctgacgggcg
     4801 atgtctgcca gagggagagt gaagcgcggg caaccgcaat ccgcgggtga ccccgctaac
     4861 tcagggcagc ggtcttcgct atcaggaacg ccatccgaat ccgtgtcgcc tgccaggggc
     4921 aaacctaacc actcaaaaag accgggcaga tcaggcgcca ggtcaaacag gtcaggtatg
     4981 aaatccaggt cagtatccac gagccaatcg tctattgggc atccatcgcc gctgccaacc
     5041 cctgcttctt ctgggcagcg gtcgacgcta tctgccacgc catcgccatc gctgtcctcg
     5101 ctcacctcta acgggcaacc tctggtgctt ctgccaccgg gctcatccgg gcaatcatcc
     5161 tcggcatccg cgataccatc cccatcggca tcgccctctg ttacatgcgg gcagcccaac
     5221 gcttctcctg ttccagcttc atcaggacag cggtcaagga agtcaggaat ggcatcacca
     5281 tcgcggtcta caaaccacga ttccaccggg cagccggcga gctcagccag gccgctctct
     5341 tctggacaga ggtcggcgcg atcaagaatg ccgtcgccgt ccgtatcagg gcgggcgtcg
     5401 ggcgggcaac ccccgtcgat agcaaaaccg aagacttccg ggcaggcgtc atcgtctcct
     5461 atcaaaccgt ctccatcaat gtccgcaggg tgcaggcagc cgtgggactc gctgtaacca
     5521 tattcctcgg ggcaaacatc gattgagtct ggtatgccat cgtgatcggt atcagcgaca
     5581 ggagtgacct caatcttctg ctggacgatg ttcatcacgc cgcgggtatt ggtggcgata
     5641 aaagcgagtt cgtgatctcc ggttaataca ggcgtccaat tccatctccc aagtaaagaa
     5701 tcgctttcac cactgacact gccgtaaagg aagccatcca ggtacaaatc caggcgtctg
     5761 atgggtacac ggcgttgttt tgccatcgct tgaatggtga aaggggtaag ggatggcgca
     5821 gtttgtcctt ttacgggaga gaggatatca atagaggggc tggaggtgac gcgcaggacg
     5881 tgaatgaaaa agaggactcc tacaaacagg agggcgagca ggagcatccc caggaggatc
     5941 cagaggattc gcttgataat tgttttcttg cgctgcaggg tggtcatttt ctctccttca
     6001 tatcaggcac attttggttg tgaaagtccg cacgccagat tgggtgaagt tgcccgcgct
     6061 gtctttcgcg atgatgtaaa actcaacaat gccgtccgcg tagaaagaga ggctgctggc
     6121 gagctgtgag attcccagcg ctgcctggta ggtggtgccg cccgtgttgc tcatggtcag
     6181 gctgcgccat tctccggggg tggcggaaga gctgtttttg gcgcggaacc acaacaccac
     6241 ttcactcaca ccagaaacat ctgtaacaga ggcgctgatg gtattactgg ctaaaccgca
     6301 ggaggtgtta tcgaagatgc tttccgctga tttggtgatc tctgtgattg acggaccctg
     6361 cgtatctggc ggagatggag gcggcacttc tggcggagac acgtagatgg tgaggctttt
     6421 ttctatgctg cccacgcggg attctccgcg cagggtgtat gtgctggaag cgaccgggca
     6481 gacctggcgg ctgtgcatca gttctacccg ctcgccgttc agataggcgc tttcaacaaa
     6541 aaccgttgac cagcgcagaa atgtgcattc tccagcggtc agcgtgtaag cgtctgcgct
     6601 gaattggata tctggcaaaa tggtgggcgg agcggaagtt gggatggcgg tatccgccgt
     6661 catgggcaga gacttcgccg tcggcgtgga atgagaagaa ggcgttggtt gggggagcac
     6721 ttttaaatag cgcgcacccg acacgacgct atctgctgaa tacgccctca ggttgtactc
     6781 cccgggtaag gtgggaaccc aaacggttac gccttcaaaa atcgttgatc cgacctggtg
     6841 catgggcact gttgccaaag ggacggcact ggaagagagc aggttgtact ggatgaaaac
     6901 ctcgctcaac tcatagttgg aatggaccgt gatggaaacg ctggtgttca cgtaggtttc
     6961 cttttcaact ggtccatcca cccagattgc atggcctggc ccgatccctc cggtgtgcag
     7021 accacagcca gccagcaata gcagccccat caatggaatg aggaatcgac agattttcat
     7081 gtgtgaacct ccctgttgcc tacccgatca ggctaagcag aaaaaagggc attgagaaac
     7141 agcgatgtgg gggtgttgag gtcaggattg tgggattgat gaagtgcgag ttaccatttc
     7201 tattatacga gaaaaagtgg agaattgaac ctgcgaggtt aacccgggtt gttgcgcaac
     7261 aacccggggc gggtaaaaaa agaggctggc gaaaatgcca gcctcagggg tgaggtgggc
     7321 gcggagggac tcgaacccac gacctcacgg atgtgaaccg tgcgctctaa ccaactgagc
     7381 cacgcgcccc tggagtacat aaaaaccctg aa
//
LOCUS       DAOTLW010000060         7369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_9778_length_7369_cov_13.837162, whole genome shotgun sequence.
ACCESSION   DAOTLW010000060 DAOTLW010000000
VERSION     DAOTLW010000060.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7369
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9778_length_7369_cov_13.837162"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..756)
                     /locus_tag="P5516_07965"
     CDS             complement(<1..756)
                     /locus_tag="P5516_07965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRT92405.1"
                     /translation="MRGARGAFNGDAGGQSYREGIDIAPDAFYRMLDEADTLPVTSQP
                     AVGEFAALYSRLAQRDPEILSIHISSGLSGTVNSARAGAAMTPEANVSVVDTKTLSAP
                     SGWQVEVAARAAAAGWSREKILETLARISSATEVLYTLKELKYLIHGGRISHLKGLIA
                     SVLNIKPLIGVEKARGTYVSLGQARSFNNAIKSVAEFVSSKVTKGTRLRAQVVHAANP
                     EGAQQLVDAIAAHFTVDWVPVRQLSLVLGAHVGR"
     gene            1077..1667
                     /locus_tag="P5516_07970"
     CDS             1077..1667
                     /locus_tag="P5516_07970"
                     /inference="COORDINATES: protein motif:HMM:NF025033.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRT92406.1"
                     /translation="MWDERYASEDYRFGTRPNRFLVDCVAGLTPAKALALGEGEGRNA
                     VFLAGLGFDVTAVDQSAVGLAKARRLAQMNGVRLHTITADLNDFVIQPAAWSLIINFF
                     CHLPSAQRAPLHQRVTAGLVPGGAYVYEVFSPAQLQLATGGPSDRDLLVSLEEARRDL
                     AALDLRVGRELVRYCEEDDPSTPRMAVLQVLAVKPA"
     gene            1770..3026
                     /locus_tag="P5516_07975"
     CDS             1770..3026
                     /locus_tag="P5516_07975"
                     /inference="COORDINATES: protein motif:HMM:NF015185.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92407.1"
                     /translation="MTAAKEKTPLLSPVLATFMLAMILANLGGNMYGPLLSLYLESLG
                     ASVTQIGLFYTLSQVVPLVLQILGGWVSDALGRLRAIAIGSVFGTIGALILVGSTSWG
                     WVLLSSAVGAGAGALVGPSFDAFIAEKSSSQNRARVFGVSQMLFGIVAVVGPPAGGWI
                     VHNYGYKTMLWVAAFLYIIAMVIRIAMARSGRHDTVTYERSAFTFAGLKKNLGAMFGL
                     LFAGGVITWILITDGVRDISFAMSMNFLGLFMEQVGGLNIQTIGLLNGVFGLFMMAFM
                     VPGGWLADKKGERLGIALGFFLNSLALGALSLLPPASPVWVYVTGFAVSGLGVGLIAP
                     AYQALISKAVPAHLRGTAFGLFSTSLGLVSLPAPFLGSLLWHHVSPRLPFAITAVVSL
                     LTILPTWLKFKLPQDVNAGDTSAPGS"
     gene            complement(3070..5004)
                     /locus_tag="P5516_07980"
     CDS             complement(3070..5004)
                     /locus_tag="P5516_07980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92408.1"
                     /translation="MKKGIVCGIGLLLLFSISCNLLNLVNKKTAEEPVQKKEILLETY
                     NTSKAYLELRLRTENILVNVGEFAGYEQWNTEMSALISDWQAFEAKASELETMAEKYA
                     NQGTAFLVDDGTGGGVVYAVHLVHQPVKAEQKYELTNIFDNAPAGKKIATLAKYLGTD
                     AKRAYALLQQEQGLITADAYNEAGDTFKKLETSAVVIKDTCKVAGFVVGTAISGGVGG
                     TLLEGSSFIIGGADMVLEIGEDGANIALGDNNKVSAIIGKARKITEPVASILTIATTS
                     FDKPIDIVNAYLFEADQLRSGFQEGKVLGISIPVPGLKKDEVETPAPTPAAQATEGGG
                     QAPTPEVNKGEVETPALTPMAQATEDGGQAGGEEEARAVIASVIEPGEISEWFEENGL
                     PMIKEPLTEAEMAEILGITLPNMGTAQKAGAAVEPTPFLAGGQSESALENAIDLSGAW
                     AIKLPDDKALREVHLSCDQRAADMLSWVTIDLTLPLVVMSGRFEPSTIEGVYKFIAPP
                     SDDNQTVTGTARMVNDEVQLEMTFSEGETTVFECDLEKHDGCEIFKYPGSNVCRFVFK
                     GLTTPEEGFNFEGYEALWKLTDGTVTCTYDRAVRDYLVYRDGVKKDERFLCQGTIVVP
                     EISAYIFNELPNYLEMFTNN"
     gene            5470..>6185
                     /locus_tag="P5516_07985"
     CDS             5470..>6185
                     /locus_tag="P5516_07985"
                     /inference="COORDINATES: protein motif:HMM:NF025035.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine-specific methyltransferase EcoRI family
                     protein"
                     /protein_id="HRT92409.1"
                     /translation="MPNEHLSDAKRAKFDEFYTQYHDIEKEVTAYTDYDQNVFRDKTI
                     LLPCDDPEWSNFTKYFAQNFKRLGLKKLISTSYAVESKTYQSAYQPTLFEIREPQFDA
                     YKTRVNGKIFILERDRTANGRTDVEDLEWRYLAGDGDFRSAEIKKLRDAADVIVTNPP
                     FSLFREFLAWIMEANKKFLIIGGMNAITYKEVFPLIKNDQMWLGNGFHAGNAFFSTPY
                     TKEYAEGVYNPETGLVKFRNV"
     assembly_gap    6186..6285
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <6286..7365
                     /locus_tag="P5516_07990"
     CDS             <6286..7365
                     /locus_tag="P5516_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRT92410.1"
                     /translation="FGLAGRLTIQPEYQRNYFYADGKKDVAVIDSILKGYPIGLIYFN
                     KVDDNQLEVLDGQQRITSFGRYVTNKFAVKDENGMEQYFSGLAADKQEKILNTPLLIY
                     ECEGTESEIKDWFKTINIAGVPLNDQELLNAVYSGPFVTLAKREFSNSQNAHIQKWSA
                     YINGAVNRQAYLERALDWVSKGNIGEYMSRHRYDNQITELTVYFNSVIDWVSSVFIDV
                     ESEMRGLEWGRLYETFHHTAYDPARVSQQVHRLYADPYIKSRKGIFEYILGGCQATHL
                     LDVRVFDQATKNSVYAQQTAAARVSGLSNCPLCALGSDANRERIWSITDMDADHVTAW
                     SKGGATDSKNCQMLCKTHNRAKGNR"
BASE COUNT         1473 a         2246 c         1876 g         1674 t
ORIGIN      
        1 gcgacccaca tgcgcgccca gcaccagcga aagctggcgc accggcaccc aatccaccgt
       61 aaaatgcgcg gcgatcgcat ctaccagttg ctgcgcgcct tcggggttgg cggcgtgcac
      121 cacctgcgcg cgcaggcgcg tgcctttggt caccttactg ctcacaaatt cagcgacgct
      181 cttgatggcg ttgttgaaag agcgcgcctg ccccaggctc acataagtgc cgcgcgcctt
      241 ctccacgccg atgagcggtt tgatgttcag taccgaggcg atcagcccct tcaaatggct
      301 gatgcgcccg ccatgaatga gatatttcaa ttccttaagc gtgtacagaa cctccgtggc
      361 gctgctgatg cgcgccagcg tttcgaggat cttctcgcgc gaccagcccg ccgctgccgc
      421 ccgcgccgcc acttccacct gccagcctga aggcgctgaa agcgttttcg tgtccaccac
      481 gctcacgttg gcttcgggcg tcatagctgc cccagcgcgc gctgaattca ctgtgccgct
      541 gagcccggaa gaaatgtgga tggaaaggat ctcggggtcg cgctgcgcca gccggctgta
      601 cagcgcggca aactccccca ccgccggctg cgaggtgacc ggcagcgtgt ccgcctcgtc
      661 cagcatccgg taaaacgcgt ccggcgcgat atcaatccct tcacgatacg attgaccccc
      721 agcgtcaccg ttaaaggcac cacgtgcacc ccgtatcgtt ccatctcttc cggggtgaag
      781 ttcacatccg ttccactgtc tgttacgatt tgcattgaat actccctgtc accaaatttc
      841 aacaccgcag gagaatcctc ggtgcgtagg ttgagtataa aacgatgaga ggcgtttcgc
      901 aatgtgccaa aggtgagatt cgtccgcctg aacttgaaag cgccccgcca tgctttccag
      961 cggcgctgtc actgagcgtg tttcagggca gcctggcacc gccgcggcgt cgcagcccgg
     1021 cgcagggttg agcaggtcgc cgcccgcggg gatatttcac gctataatgt ctccctatgt
     1081 gggatgaacg ctacgcttca gaggattacc gcttcggcac gcgccccaac cgttttttgg
     1141 tggattgcgt tgccgggctg acccccgcga aagcgctcgc cctcggcgag ggcgaagggc
     1201 gcaacgctgt ttttctggcc ggcttgggct ttgatgtcac cgccgtggac cagtctgccg
     1261 tggggctcgc caaagcccgc cgcctggcgc aaatgaacgg cgtgcgcctt cacaccatca
     1321 ccgccgacct caatgacttc gtcatccagc ccgcggcgtg gagcctgatc atcaatttct
     1381 tctgtcacct gccctccgcg cagcgcgcgc cgctgcatca acgcgtgacc gcggggcttg
     1441 tacccggcgg cgcctacgtg tacgaggtct tctctcccgc ccagctgcag ctggctaccg
     1501 gcggacccag cgaccgcgac ctactggtat cgctggaaga agcccgccgc gacctggccg
     1561 cgctcgacct gcgcgtgggc cgcgagctcg tgcgctactg tgaagaggac gacccctcca
     1621 ccccgcggat ggccgtgctg caggtgctgg cggtgaagcc cgcgtagccg ccaaccctcc
     1681 cggctcctgc tccgcctggc gcaactttct gcgccaatct gcgtatatcc tgctagaata
     1741 tatccctacc tcttttttaa ataccgccca tgaccgccgc caaagaaaaa acccccctgc
     1801 tctccccggt gctcgccacc ttcatgctgg ccatgatcct ggccaacctg ggcggcaata
     1861 tgtacgggcc gctgctctcg ctctatcttg aatccctggg cgccagcgtc acccaaattg
     1921 gacttttcta caccctttcg caggtcgtgc cgctggtgct gcaaatcctc ggcggctggg
     1981 tatcagacgc cttgggcagg ctgcgcgcca ttgccatcgg cagcgttttc ggcaccattg
     2041 gcgcgctcat cctcgtcggc tctaccagct ggggctgggt gctgctttcc agcgcggtgg
     2101 gcgccggggc gggggcgctg gtcggcccca gttttgacgc cttcatcgct gaaaaatcct
     2161 ccagccagaa tcgcgcccgc gtcttcggcg tttcgcaaat gctcttcggc atcgtcgcgg
     2221 tggtgggtcc gcccgccggc ggctggatcg tgcacaatta cggctacaag accatgctct
     2281 gggtcgccgc cttcctctac atcatcgcca tggtcatccg catcgccatg gcgcgctcgg
     2341 gccggcacga tacggtcacc tacgagcgca gcgcgttcac cttcgccggt ctgaaaaaga
     2401 acctgggcgc catgttcggc ctgctcttcg ccgggggcgt catcacctgg atcctcatca
     2461 ccgacggcgt gcgcgatatc tcttttgcca tgtcgatgaa ctttttaggg ctgttcatgg
     2521 agcaggtcgg cgggctcaac attcaaacca tcgggttgct caacggggtt ttcggtctgt
     2581 tcatgatggc gttcatggtg cccggcggct ggctggcgga taagaaaggc gaacgcctgg
     2641 gcatcgcgct gggtttcttc cttaattccc tcgcgctggg cgccctctcc ctcctgccgc
     2701 ccgccagccc ggtgtgggtg tacgtcaccg gcttcgcggt ctccggcctg ggcgtggggc
     2761 tcatcgcccc cgcctaccag gcgctcatca gcaaggccgt gcccgcccac ctgcgcggca
     2821 ccgcctttgg tctcttctcc accagcctgg ggctcgtctc tctgcccgcg ccctttctcg
     2881 gcagcctgct gtggcatcac gtctccccgc ggctgccctt cgccatcacc gctgtggtca
     2941 gcctgctcac catcctcccc acttggctca aatttaaact cccccaggat gtcaacgccg
     3001 gcgataccag cgcgccaggc tcataaaccc ctgttgacgc tcgcgcaacc ctctttttcg
     3061 tgctgatgct taattattcg tgaacatctc taaataattg ggcagctcat tgaagatgta
     3121 ggcgcttatt tcaggaacga cgatcgttcc ctggcataag aaccgctcgt cttttttaac
     3181 accgtcccgg taaaccaggt aatctcttac cgctcgatca taagtacacg tgaccgttcc
     3241 gtcggtcaat ttccacagcg cctcgtatcc ttcaaaattg aacccttcct caggcgtggt
     3301 caaccccttg aagacaaacc ggcaaacatt tgatcccgga tatttgaaga tctcgcatcc
     3361 atcgtgcttt tccagatcac attcaaaaac agttgtctca ccctcagaga aggtcatttc
     3421 taattgcact tcatcgttga ccatccgcgc agtaccggtg accgtttgat tatcgtcgct
     3481 tgggggtgca ataaacttgt aaacgccttc aatggtggag ggctcaaaac gacccgacat
     3541 aaccaccagg ggcagcgtta aatcgatcgt cacccaactc agcatatcag cggcgcgttg
     3601 atcacaagaa agatgaactt ccctcagggc tttatcgtca gggagcttaa tcgcccaggc
     3661 gcctgagagg tctatagcgt tctccaacgc gctctcgctt tgtcctccag ccaaaaacgg
     3721 agtgggttca accgccgcgc cggctttttg tgctgtaccc atgtttggca gtgtgatccc
     3781 tagaatttcg gccatctccg cttccgttaa tggttccttt atcatgggta agccgttctc
     3841 ttcgaaccac tcgcttatct cgcccggctc aattacgctg gcgatgaccg ctcgcgcttc
     3901 ctcctcgcct cccgcctgac cgccgtcctc ggtagcctga gccataggtg taagcgcggg
     3961 tgtctccact tctcccttgt taacttcagg ggtcggagcc tgaccgccgc cctcggtagc
     4021 ctgagccgcg ggagtaggag cgggcgtttc cacttcatcc tttttaagtc cagggaccgg
     4081 gatggaaatg cccagcactt tcccttcttg aaaacctgac ctcaattgat cggcttcgaa
     4141 caggtatgcg ttgacaatat cgatgggttt atcaaaactg gtggtggcaa tggtgaggat
     4201 ggacgccact ggttcagtga ttttgcgcgc cttgccaatg atcgctgata ccttgttgtt
     4261 atcccccaag gcgatattgg ccccatcttc ccctatttcc agcaccatat cagcgccgcc
     4321 aatgataaag gaggatccct cgagcagcgt tcctcccacc ccgccagaga tggctgtgcc
     4381 aaccacaaaa ccagccacct tgcaggtgtc ttttatcacc accgcgcttg tttcaagctt
     4441 tttgaacgta tcgcccgctt cattataggc gtcagcggtg atgagccctt gttcctgctg
     4501 cagcagcgcg taggcgcgtt tggcgtccgt gccaaggtat ttcgccaggg tggcgatctt
     4561 ttttcccgcg ggcgcgttat cgaaaatatt ggtcaattcg tatttttgtt ccgcttttac
     4621 cggttgatgg accaggtgca cggcgtaaac cactcccccc cctgttccat catccacgag
     4681 gaaggcggtt ccttggttgg cgtatttttc tgccatcgtt tcaagctcgc ttgctttcgc
     4741 ttcaaacgcc tgccaatcgc tgatcagcgc gctcatctct gtgttccatt gttcgtaccc
     4801 cgcgaattcc cccacgttca ccaggatatt ctctgtgcgt aagcgcagtt caagatacgc
     4861 cttggacgtg ttgtaggttt ccagcaagat ttcttttttc tgcacgggtt cctccgccgt
     4921 ctttttattc accaggttca acaaattaca actgatggag aaaagcagga gtaaccctat
     4981 gccgcagacg atgccttttt tcatttcgac ctccctaaag aacacgcgtt ggaaaatgaa
     5041 tttttagacc ggcggatcaa aaacgcgcca gtcaggcgaa agcctttcca tttcaaccag
     5101 taacctgttt tacgctgaat ctgtgacgga aaacggtgcg ctgaaaaata tcaggggtgt
     5161 ctgtcaacca ggctgaccaa atgtcgctgc tattccctct tgatggtgta attcggtttt
     5221 accccgggcc cacgcctgat attcttcgcc gataacttca ccctggccgg gtgatgaggt
     5281 tggctgaaca cactcgcgtt cagcagggtc acctttattg tacagcattt agcgggcttt
     5341 gttcaacccg ggcgcgatgg tccccttttt gccaaagtgg atgcgtcccc ttccccgctt
     5401 cagccgcgcg catgggtggc agggaaagcg ctataattac tgtatcaatt cggtttgccg
     5461 gggtagccaa tgccaaacga acatctctca gacgccaaaa gggcgaaatt cgacgagttt
     5521 tatacccaat accatgatat cgaaaaagag gtcaccgctt acaccgatta tgaccaaaac
     5581 gtgttccgcg ataagaccat cctgctgccc tgcgacgacc ccgaatggag caattttacc
     5641 aagtacttcg cccagaattt taagcgcctc gggctgaaaa aactcatcag caccagctac
     5701 gccgtggaaa gcaagaccta tcaaagcgcc taccagccca ccctctttga gatccgcgaa
     5761 ccgcagtttg acgcttacaa gaccagggtc aacggcaaga tctttatttt ggagcgcgac
     5821 agaaccgcca acggcagaac ggacgtggaa gacctcgagt ggcgttacct ggcgggcgat
     5881 ggcgattttc gcagcgccga gattaagaaa ctgcgcgatg cagccgatgt catcgtcacc
     5941 aacccgcctt tctccctctt ccgcgagttc ctggcctgga ttatggaggc gaataaaaaa
     6001 ttcttaatta ttggaggtat gaatgccatt acctataaag aagtgttccc attaatcaag
     6061 aacgatcaaa tgtggcttgg caatggtttt catgccggga atgctttctt ttccactcct
     6121 tacaccaaag aatatgcaga aggtgtttac aatccagaga ccggtttggt caagtttagg
     6181 aacgtnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     6241 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnttcgg tcttgccggg
     6301 cgcctcacca tccagccgga ataccagcgc aactattttt acgccgatgg gaaaaaagac
     6361 gtggctgtca ttgattcgat cctgaagggt taccctatcg gtttgatcta tttcaataaa
     6421 gtagatgata accaattgga ggtcctcgac ggacagcagc gcatcaccag ttttgggcgt
     6481 tatgtcacca ataagtttgc cgtcaaagat gaaaacggca tggagcagta tttcagcggt
     6541 cttgccgctg ataagcagga aaaaattcta aataccccgc tgctcattta tgaatgtgaa
     6601 ggcactgaaa gcgagatcaa agactggttc aagaccatca atatcgccgg tgttcccctt
     6661 aatgatcagg aactgctcaa cgcggtctat tctggaccgt ttgtcactct tgccaaaagg
     6721 gaattcagta acagccaaaa tgcccatatc caaaaatgga gcgcctacat caacggcgcg
     6781 gtcaatcgtc aggcctatct tgagcgtgcc cttgattggg tgagcaaagg caacatcggc
     6841 gaatatatga gccgccaccg ttacgataac cagattaccg aattgaccgt ctattttaac
     6901 agcgtcatcg attgggtttc ttccgtgttc atcgacgtgg aaagcgaaat gcgcgggctg
     6961 gaatggggtc gcctgtacga aacattccat cacaccgctt atgacccggc gcgcgtttct
     7021 caacaggtgc accgccttta cgccgacccg tacatcaaaa gccgcaaggg catctttgaa
     7081 tatatcctcg gcggctgcca ggccacccac ctgctcgacg tgcgcgtttt tgaccaggcg
     7141 accaagaaca gcgtctacgc ccaacagacc gccgccgcca gggtcagcgg gttgtccaac
     7201 tgcccgcttt gcgctctcgg gtcagacgcc aaccgcgaga ggatatggag catcaccgac
     7261 atggacgccg accatgtgac cgcgtggagc aagggcggcg ccaccgatag caagaactgc
     7321 cagatgctgt gcaaaacgca taaccgcgcc aaaggcaacc gttaaagcc
//
LOCUS       DAOTLW010000061         7351 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_9815_length_7351_cov_11.123355, whole genome shotgun sequence.
ACCESSION   DAOTLW010000061 DAOTLW010000000
VERSION     DAOTLW010000061.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7351)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7351
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9815_length_7351_cov_11.123355"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="P5516_07995"
     CDS             complement(<1..800)
                     /locus_tag="P5516_07995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRT92411.1"
                     /translation="MTEFILVAAAWPYANAEIHVGNLCGSYLPADIFARYHRLRGRRV
                     SFVSGTDSHGTPITVRAEAEKSTPYQVYQRFHSGFLNLFQQLGLTYDLFTSTHTQNHM
                     HVARQVFLALKHNGFLYKESQDQWYSVSQQRFLPDRYVEGTCYICGYDGARSDQCDKC
                     GNMLEASQLLNPKSKIDGSTPELKSTEHYFLDLSRLQDLVVEFLKVRESYWRPNVLRQ
                     SLGQIQANGLRGRPITRDLEWGIKLPAEALGEGPEWENKCLYVWFEAV"
     gene            958..2148
                     /locus_tag="P5516_08000"
     CDS             958..2148
                     /locus_tag="P5516_08000"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing M23
                     family metallopeptidase"
                     /protein_id="HRT92412.1"
                     /translation="MMDNSDKNTAGEAQQLSENPADEAFSPAERLWNRILHLGLGETT
                     LRVGTAVVSLLLVLFVVWVMGRFVLNNQNKPTDQPVGAEELNLQTPATPLIMKAEPVL
                     YSGFGISRLAITHTDRPAQPRNSIIEYTIVAGDTLFGIAERFGLQPQTLLWSNRHILG
                     DDPHNIFPGVKILIPVIDGAVYKWNTGDGLNGVASFYHVTPEAIVDWPANQLNRETLG
                     DFAMPNIPEGTLLFVPGGVGEFTDWLPSYTREKPAESSISGSACGIQTSGYIGSGPPF
                     MWPTSETYISGYQYSPETNHRAIDIGGKIGNPVYAVDDGVVVYSNWNQSGYGNLIVID
                     HGNGWQSVYAHLDNYLSFCGDNVVRGQEIGILGTTGNSSGPHLHFELRHENYGAVNPW
                     DFLE"
     gene            complement(2221..2748)
                     /locus_tag="P5516_08005"
     CDS             complement(2221..2748)
                     /locus_tag="P5516_08005"
                     /inference="COORDINATES: protein motif:HMM:NF003191.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HRT92413.1"
                     /translation="MAYLSEPHAWLFALMIFALRVVDMSLDTIRVLFVVRGKKLIVWV
                     LGFFQSLVFVVAISSVLTQLNNILNVIGYATGFATGNLVGMLIEQRLAIGHILITIIS
                     SSRGSYIAEQLRSNGYAVTEIAGRGRDGTVFELHASVLRRDVPQVETIVLEADPQAFI
                     TAEDVRPVRRGFWRA"
     gene            complement(2761..4923)
                     /gene="glgP"
                     /locus_tag="P5516_08010"
     CDS             complement(2761..4923)
                     /gene="glgP"
                     /locus_tag="P5516_08010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucan family phosphorylase"
                     /protein_id="HRT92414.1"
                     /translation="MTIKIIREYPNSYNLPKRIEGLTRLAHNMWWVWNHDGQRAFQFI
                     DPRLWDLVAHNPVAFLRRVERHRLNAAVGQSAYLEHYDRTLANFDAYIADRNTWFQRT
                     YPQFTDKTIAYFSFEFGIHESLPVYAGGLGILSGDHLKEASDLGLPLVGMGFVYDKGY
                     FVQELTEDGWQEPRNYLIDYNMLPLIALLDENRKPLTVTIDLPGRQVSARLWELEVGR
                     NHLYLLDSNVETNAPADRQLTERLYSNDLEVRISQEMLLGMGGVQALRLLGYDPGVWH
                     MNEGHSTFLALERIREKMKTGTRFQAAWEEVKVTDVFTTHTPVPAGNDQFPLWLVDKY
                     FGNYWPELGLTHDQFLDLGRQAQSWGDTFVMPVLALKLSERANAVSELHGIVSRRMWH
                     FLWPDREVEDVPIHYVTNGVHTRSWLARRMGVLFDTYLGEDWREHLDEPDFWNNINRI
                     PNEELWAVRRHLKNKLVNFTNERTRQQWQNGQLTPSQAVAGGVFMEPYSLTIGFARRF
                     ATYKRANLVFKDFDRLMNIINDAHMPVQIVFAGKAHPADEPGKLLIQQVYRAVKDTRS
                     AGRLVFIEDYDINVARYLVQGVDIWLNTPRRPNEASGTSGMKAALNGVLNFSILDGWW
                     REGYNGKNGWAIGKEIEYTDSEAQDAADAQSLYETLEKQIVPLYYERDENKIPQEWLK
                     MVKECLRTLVPHFSLRRMLKEYTTDYYLPAMKQEKAEW"
     gene            complement(5095..5493)
                     /locus_tag="P5516_08015"
     CDS             complement(5095..5493)
                     /locus_tag="P5516_08015"
                     /inference="COORDINATES: protein motif:HMM:NF014132.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCP2 sterol-binding domain-containing protein"
                     /protein_id="HRT92415.1"
                     /translation="MTEQMFMSPEWMAETEKILRAMITPQTTHNASLSLTMTVENLPG
                     GETKTLVFATDKGKLKTFRLADPGSVNTEFTLSGDYRVYEKLFKGQLEPASAIMGGEL
                     KFSGNMLKAIRLLPTLEPFFAVFSKVPTRF"
     gene            complement(5526..6857)
                     /locus_tag="P5516_08020"
     CDS             complement(5526..6857)
                     /locus_tag="P5516_08020"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012457.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HRT92416.1"
                     /translation="MPEAIFQFPRGFLWGCATAAHQVEGGNSNNNWSKWEETPGKIVN
                     GHRSGLACDWWGGRWLEDLERARDSGQNAHRLSIEWSRIQPEPDRWDDEALAKYTEML
                     RWMTRNGITPLVTLHHFTDPLWLSERGGWESEDTPALFARFCRRVVKTLKDYVNLWVT
                     INEPNVYILSGWVEGVFPPGKQDLKLAARVFTNLVQGHSAAYRIIHELQPEARVGVAT
                     NYRSFAPAHPGSPLDKLPLGFVNQVFNRAFNNCLVDGKVDLVFKKYKLPQAAGTQDFI
                     GLNYYTRDVVSFNLLKPGAFFHQRAFPAGAALSENHFIANVPAGMWDGLKWAHAYGIP
                     IIVTENGIEDSRDTLRPRYLVEHLHQVWRAANYNYQLKGYFHWSLVDNFEWERGWTQR
                     FGLWGLDVDTQQRNRRRSVDVYERVCRENGLSSHMVAELVPDVLDALFPDH"
     gene            complement(6950..>7351)
                     /locus_tag="P5516_08025"
     CDS             complement(6950..>7351)
                     /locus_tag="P5516_08025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HRT92417.1"
                     /translation="KAYDHIGVYFVVDTLKYLHAGGRIGGGKRFMGTALNIKPILEIR
                     EGKIEAVKSVISINKALEAMVQLVEADIQGRSPVRISVFHALAEAKAQALLESAAKRL
                     KADEAILSYVSPVIGCHVGPGTLSIAYMAGM"
BASE COUNT         1663 a         2036 c         1988 g         1664 t
ORIGIN      
        1 atcaccgctt caaaccatac gtaaaggcac ttgttctccc attccggacc ctctccgagg
       61 gcttcggcgg gaagcttaat cccccattca aggtcgcggg tgatgggacg cccgcgaaga
      121 ccatttgcct gtatctgtcc aagcgactgg cgcagcacgt tggggcgcca gtaactttca
      181 cgtactttaa gaaattccac caccaggtct tgcaggcggg aaagatccag aaagtaatgc
      241 tcggtgctct tcaactccgg ggtgctgccg tcgatcttgg atttagggtt gagcagctgc
      301 gaggcttcga gcatgttgcc gcatttgtcg cattggtcgc tgcgcgcgcc atcatacccg
      361 cagatataac aggtaccctc cacgtagcga tcaggcagaa agcgctgctg gctaacggaa
      421 taccactggt cctgactctc tttataaagg aagccgttgt gttttaatgc caggaaaacc
      481 tggcgtgcca cgtgcatgtg attttgggta tgcgtactgg tgaacagatc gtaagtcagg
      541 ccgagctgct gaaagaggtt gagaaacccg ctgtggaagc gctggtacac ctggtagggg
      601 gtgctttttt ccgcttcggc gcggacggtg atgggtgtgc cgtgactatc cgttccggag
      661 acgaaactca cccggcgtcc gcgcaggcgg tgatagcgcg cgaagatatc tgctggcagg
      721 taagaaccgc acaagttgcc cacgtgaatt tcagcattag cgtaaggcca cgccgctgca
      781 acaaggataa attccgtcat gctcacctcg cttacttgat ggcggtattc taacacgaaa
      841 cctggcgcgg gcgcacccgc ccttgcagtt aatctcacct ccaaaccctc ggcccattcg
      901 atttttcaaa acttgaccat attgatgcgc gatgttatga tttttttcga ggtaaatatg
      961 atggacaact cagataaaaa tactgcgggc gaggctcaac aattatcaga aaaccccgcc
     1021 gacgaggctt tttcgccagc agagaggtta tggaatcgca tcctgcacct cgggctgggc
     1081 gaaaccactt tacgcgtggg caccgcggta gtctccctct tactggtgct cttcgtggtg
     1141 tgggtgatgg gcaggttcgt gcttaacaac cagaacaaac caaccgacca gccagtgggg
     1201 gctgaagaac tgaacctgca aaccccagcc acacctttga ttatgaaagc cgagccagtg
     1261 ctctacagcg ggtttgggat cagccggctg gcgataaccc acacagaccg cccggcgcag
     1321 ccgcgcaaca gcatcatcga gtacaccatt gtggcgggtg atacgctttt cggcattgcg
     1381 gaaagatttg gcctgcaacc ccaaacctta ttatggagca atcgccacat cctgggtgat
     1441 gacccgcaca atatcttccc cggggtgaaa attctcattc cggtgataga tggtgcggtt
     1501 tataaatgga acacgggtga cggcctgaac ggcgtcgcca gtttctatca cgtcacccct
     1561 gaggccatcg tcgattggcc ggccaaccaa cttaaccgcg aaacgctggg cgatttcgcc
     1621 atgcccaaca tccctgaagg cactctgctc tttgtccctg ggggagtggg tgagttcacc
     1681 gactggctgc ccagctacac gcgagaaaaa cccgccgagt cttccatctc aggcagcgcc
     1741 tgcggcatac agacctctgg ttacattggt tctggtccgc cgttcatgtg gccaacttca
     1801 gagacgtaca tctccgggta tcaatacagc cccgaaacca atcaccgcgc cattgatatc
     1861 ggcggtaaga ttggtaaccc tgtttacgcg gtggatgacg gcgtggttgt ttactccaac
     1921 tggaaccaaa gcgggtatgg caacctgatt gtaattgacc atggcaatgg ctggcaatcg
     1981 gtctacgctc acctcgataa ctacctcagc ttttgcgggg ataatgtggt gcgcggacag
     2041 gagatcggca tactgggcac caccggcaac tccagtggtc cacacctgca ctttgaactg
     2101 cgccacgaga actacggcgc cgtgaacccc tgggacttcc tggaataggc ggatgcatgc
     2161 acaaagaccc gggatggtaa gccccgggtc tttttcatta agcgatacaa ggtaaaccgc
     2221 tcaagcccgc cagaacccac gccgcaccgg gcgcacgtct tcggcggtga taaaggcctg
     2281 tgggtcagcc tcgagtacga tggtctctac ttgagggaca tcgcggcgca atacactggc
     2341 gtgcaactca aagacggtgc cgtctctgcc gcgcccggcg atttcggtga cggcatagcc
     2401 attgctgcgc aactgctcag cgatatacga accacggctg gagctgatga tagtgatcag
     2461 gatgtggccg atggccaggc gctgctcgat gagcatgcca accaggttgc cagtggcgaa
     2521 gccggtggcg tagccgatga cgttaaggat attgtttaac tgtgtaagca ccgaactgat
     2581 ggcgacgacg aaaaccaggg attgaaagaa accaagcacc caaacaatga gctttttccc
     2641 gcgcaccacg aacagcacgc ggatggtatc caacgacata tccacaacgc gcagcgcgaa
     2701 gatcatcaag gcaaaaagcc aggcgtgagg ttcagataag tatgccattt tattcctctc
     2761 ttaccattcc gctttctcct gcttcatcgc cggcaggtag taatcggtgg tgtattcttt
     2821 taacatgcgg cgcaggctga aatgcggtac gagcgtgcgt aaacactcct tgaccatctt
     2881 taaccattct tgtgggattt tgttctcatc gcgctcataa tacagcggga cgatctgttt
     2941 ttcaagcgtt tcataaaggc tctgcgcgtc cgcggcatcc tgtgcttcag aatcagtgta
     3001 ctcgatttct ttaccgattg cccagccgtt cttgccattg tacccttcgc gccaccagcc
     3061 atccaaaatc gaaaaattca gcacaccatt gagagccgcc ttcatgcccg aagtgcctga
     3121 agcttcgttg gggcggcgag gggtgttcag ccagatgtct acaccctgca ccaggtaacg
     3181 ggcgacatta atgtcataat cttcaatgaa aaccaggcga cctgcgctgc gcgtatcttt
     3241 taccgcgcga tagacctgct gaatgagcag tttgccgggc tcgtcggcgg ggtgggcttt
     3301 accggcaaag acgatctgca ccggcatgtg agcgtcattg atgatattca tcaggcgatc
     3361 gaaatccttg aataccaggt tggcgcgctt gtaagtggca aagcggcgtg caaaaccaat
     3421 cgtcagcgaa tagggttcca taaaaacgcc cccggcgacc gcctggctgg gggtgagctg
     3481 accgttctgc cactgctggc gggtgcgttc attcgtgaag ttcaccagct tattctttaa
     3541 gtgacggcgt accgcccaca gttcttcatt gggaatacgg ttgatgttgt tccagaagtc
     3601 gggttcatcc agatgttcac gccaatcttc gcccaggtag gtatcaaaaa gcacacccat
     3661 gcgccttgcc agccacgagc gcgtatgcac cccgttggtc acatagtgga tcggcacatc
     3721 ttcaacctcg cggtctggcc agaggaagtg ccacatcctg cgcgaaacga tgccgtgcag
     3781 ctcagaaacc gcgttcgccc gttctgatag tttgagcgcc aataccggca tcacaaaggt
     3841 atcgccccat gattgcgcct ggcgcccgag atcaagaaac tggtcatggg ttaaccctag
     3901 ttcaggccaa taattgccaa agtacttgtc taccagccaa agcgggaact ggtcattacc
     3961 ggctggcaca ggcgtgtggg tggtgaaaac atcagtaacc ttaacctctt cccaggcggc
     4021 ttgaaaacgc gtgccggttt tcatcttctc acggatgcgt tcaagcgcga ggaaagtgga
     4081 gtgtccttcg ttcatatgcc agacccctgg gtcgtaaccc aacaggcgca gcgcctgaac
     4141 gccacccatg cccagcagca tctcctgcga gatgcgcact tcaaggtcat tactgtaaag
     4201 gcgttcggtg agctgccggt ccgcgggcgc attggtctcc acgtttgaat ccagcaggta
     4261 aagatgattg cgccctactt ccagttccca caggcgggcg ctcacctgtc gcccgggaag
     4321 gtcgatggtc acggtgaggg gtttacggtt ttcatccagc agcgcaatga gtggcagcat
     4381 attgtaatcg atgaggtaat tgcgcggttc ctgccagccg tcttccgtaa gttcctgcac
     4441 aaaataaccc ttgtcgtaca caaaacccat ccccaccagg ggcagcccca ggtcagaggc
     4501 ttctttcaga tgatcaccgg aaaggatgcc cagaccgcca gcgtacaccg gtagagactc
     4561 gtgaatacca aactcgaacg aaaaataggc gatggtttta tctgtaaatt gtgggtaggt
     4621 gcgctgaaac caggtattac gatcagcgat gtaagcgtca aagttcgcca gggtgcggtc
     4681 gtagtgttcc aggtaggcag attgccccac cgcggcgttg aggcggtgac gctccacacg
     4741 acgtaaaaaa gccactggat tgtgggctac caggtcccac aggcgcgggt caatgaactg
     4801 gaaagcgcgc tgcccgtcat gattccaaac ccaccacata ttatgcgcta ggcgggtcaa
     4861 cccttcgatg cgttttggca ggttatacga gtttggatac tcgcgaatga tctttatggt
     4921 cattttactc tctctactta tagatagaat cggtcaccgg gcacaattca cccatgaatc
     4981 accacgtacc ttatgcagct cgaatcgatg ggattttttg ccgggaggat tataccagat
     5041 tgtgacccag ggaagatttg cgcctcgcag aggttcatta aaaaaaaacg gttgttagaa
     5101 cctggtggga acttttgaga aaacggcgaa gaacggctca agggttggta aaagccttat
     5161 ggctttaagc atgttgcctg agaatttgag ctcgccaccc ataatagcag aagcgggctc
     5221 cagttgacct ttgaataact tctcatacac gcggtaatct ccgctgaggg tgaactcggt
     5281 attcacgctg cccgggtctg ccagccggaa ggttttcagc ttacctttat cggtagcgaa
     5341 gaccagggtc tttgtctctc cgccgggaag gttttccact gtcatcgtga gagacaggct
     5401 ggcgttatgg gtggtttgcg gggtgatcat cgcgcgcagt atcttctctg tttctgccat
     5461 ccattcaggg ctcatgaaca tctgttcagt caaggtcaac ctcctcttct attcaccaac
     5521 actgcctaat ggtcggggaa cagcgcatcc aatacatcgg gcaccagttc tgcgaccatg
     5581 tgactggaaa gcccgttttc gcggcaaaca cgttcataga catccacact gcgtcggcgg
     5641 ttgcgctgct gggtgtccac atccaacccc cacaagccaa aacgctgcgt ccaacctcgt
     5701 tcccattcaa agttatcaac caatgaccaa tggaaatagc cctttagttg ataattataa
     5761 ttggctgccc gccacacctg gtgcaggtgt tccaccaggt agcgagggcg cagcgtgtcg
     5821 cgcgaatctt caattccatt ctcggtgacg atgatcggaa tcccgtacgc gtgcgcccat
     5881 ttgagcccat cccacatgcc cgcgggcacg ttggcgataa aatggttctc gctcagcgcg
     5941 gcgcctgctg gaaacgcccg ctggtggaag aaagcgcctg gtttcaacag gttgaaagac
     6001 accacatcgc gggtgtaata atttaacccg ataaaatcct gtgtgccggc agcctggggt
     6061 aatttatact ttttgaaaac caggtctact tttccatcca ccaggcagtt attgaaagcc
     6121 cggttaaaga cctggttgac gaaacccagc gggagcttat ccaatggaga gccggggtgg
     6181 gcaggggcga agctgcggta attggtggca acccccacgc gcgcttcggg ctgaagctca
     6241 tggatgatgc ggtaagcggc gctgtgcccc tggaccaggt tggtgaatac cctggcggcg
     6301 agtttcaggt cttgcttgcc cgggggaaac acgccttcca cccaaccgct caaaatgtag
     6361 acgtttggct cgttgattgt gacccacagg ttgacatagt ctttgagcgt tttgaccacg
     6421 cgacggcaaa agcgggcgaa aagagcgggg gtgtcttcgc tttcccaacc ccctctttca
     6481 ctcaaccata atgggtcagt aaaatgatgc agcgtgacca gcggcgttat accgttgcgc
     6541 gtcatccagc gcagcatttc cgtgtacttc gccagggctt catcgtccca gcggtcgggc
     6601 tctggctgaa tgcggctcca ctcgattgaa aggcggtggg cgttctgccc gctatcgcgc
     6661 gcccgttcca ggtcttccag ccagcgaccg ccccaccaat cacaggccag ccctgaacga
     6721 tgaccattga cgatttttcc gggggtctct tcccattttg accagttgtt gttgctgttt
     6781 ccaccctcca cctggtgggc ggcggtggcg caaccccaga gaaagccgcg ggggaattgg
     6841 aagatcgctt cgggcatgcg ctgctcctgt attctttgtg attgcagcta attatagccg
     6901 aaacaaaaac cccggctcaa ccggggttaa aatgcgttgg ctgaaaggat cacatccctg
     6961 ccatataggc gatgctcagc gtgcctgggc cgacgtgaca gccgatcaca gggctgacgt
     7021 aactgaggat ggcttcatca gccttcagtc gtttagcggc tgattcaagc agcgcctggg
     7081 cttttgcttc tgccagcgcg tggaacacag agatgcgcac cggtgagcgg ccctgaatat
     7141 ctgcttcgac cagctgcacc atggcttcaa gcgctttgtt gatggagata acacttttca
     7201 cggcctcgat tttgccctct cggatttcga ggataggctt aatgttgagc gctgtgccca
     7261 tgaaccgttt accgccacca atgcgcccgc cagcgtgcag gtatttgagg gtgtccacca
     7321 caaaatacac cccgatatgg tcataggctt t
//
LOCUS       DAOTLW010000062         7280 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_9905_length_7280_cov_10.509619, whole genome shotgun sequence.
ACCESSION   DAOTLW010000062 DAOTLW010000000
VERSION     DAOTLW010000062.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 7280)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7280
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_9905_length_7280_cov_10.509619"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..944)
                     /locus_tag="P5516_08030"
     CDS             complement(<1..944)
                     /locus_tag="P5516_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024824.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT92418.1"
                     /translation="MLILAGLFVIQAVRDGRIAPILQIIPTPTRSLNSYVQEGETHFQ
                     AGNLEKAIEAYRYAIAIDPEDADLRAELARIQVYSTSQITVDARKRERLEEALATIEE
                     GLAISPQNSNLYAVKAFALDWYGSSGAAGEASQEYLVKGEQAATEALQYDNTNALALA
                     YYAELLIDQLKYSQAQQYITQALERDDTLMDVHRVNGNVQEASANYTQAIEEYKKAIQ
                     ITPNLNFLYLSLGANYRQLANMTGITTEQNYYFGLALEAFAKAVAINDQLGVRDPIPL
                     ISIANTYVQQGEFFAAARNMLRAVAFAPDDPTAYGQLGV"
     gene            complement(1054..2310)
                     /locus_tag="P5516_08035"
     CDS             complement(1054..2310)
                     /locus_tag="P5516_08035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HRT92419.1"
                     /translation="MQKKKRRSNPFRVILLIVLIAAAIYFDRMIVPTIPQFFLPTPTP
                     TRAPESITSDAKGLEAEGKYPLAIAAYKDAVRADPRNPHNFIALARLNIYTGNYEEAV
                     TDAENALLLNPNNDEAMALRGWAIGLTGDYVRGTGILQEAIAANPDYAAAYAYLAEIY
                     TYMLQAGQGDLQTLDNAINASRRAEELAPGTLETHRGRGMVLEYTSNFKEAVAEFEAA
                     VALNPNIADLHLGLGRNYFNIQEYDDAITEFGRANALNPRDPTPESLIARTYALNGDY
                     QKAIQYGEAAIMDEPQNARLYGNLGLIYSSAKQYLDAIDMLRIAVRGGVSSDGAPVEG
                     IPMDYGRVAQYYYTYGLALARVGQCGEALEISQAVQMGLRNDDIAVFNAEEMVNICER
                     LAREGKSDIAATATPAETTPTVEAAP"
     gene            2601..3314
                     /gene="trmD"
                     /locus_tag="P5516_08040"
     CDS             2601..3314
                     /gene="trmD"
                     /locus_tag="P5516_08040"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N(1))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HRT92420.1"
                     /translation="MQFDVFTLFPGVLRPYLDSSILQRAQQNDLIKVALHNIRDWTFD
                     RHHVTDDAPYGGGGGMVMKVEPIFAAVESVLGAPPSCPLLYLSPQGKPFTTAIARELA
                     QQPRLGLLCGRYEGVDERVIEHLVTEQISIGDYVLTGGELPALVLIDAVSRFIPGVLG
                     DPDGAEEDSFGNGLLEYPHYTRPEEFRGWRVPEVLISGNHALVAKWRREQSLLRTQKQ
                     RPDLLNGESKVQSTTKTKE"
     gene            3318..4640
                     /locus_tag="P5516_08045"
     CDS             3318..4640
                     /locus_tag="P5516_08045"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92421.1"
                     /translation="MEKKKFPWGRTFLVGFGFFGISILWPIFNQWVPLILQAGNPGFK
                     TNIPNFVGFGIGPALAMFIMTWDNIVNIFVQPWVGNRSDHTWNRFGRRKPWILLGLPI
                     ALVGFISIPLAQSVLAVAVFIMVTNVGMALFRSPVVSWLGDLFLPEQRSKANGVINLM
                     GGAAGVLSYVLGGLIFNAFGRMGPFVAGAVAMTVALAAVLIWVKEPKERLGKHTEDED
                     SSGAVKGLVPNLKAVFSNQDKSTLFILLAIFCWFTGFNAIETGLSSFAVFDLGIAPGK
                     ASIVASIANLSFLAFCIPSGILAGRLGRKRTILYGVFALTLIFLISFFLVKGLISFII
                     ILVLVGFFWSWVNVNSLPLVYDHGDEKRIGAYTGLYYFFSQTAAVLGPTLGGLLVERI
                     HFNYKVLFIFGAVFLFLAFLSMLKVKDRAHEEKMKLGAGIGASSSASE"
     gene            4781..>5430
                     /locus_tag="P5516_08050"
     CDS             4781..>5430
                     /locus_tag="P5516_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HRT92422.1"
                     /translation="MSKKVMLVMSVLLIAVFVLSACAKTAGPAKFKACEVTDTGGVDD
                     KSFNATAWKGAVDAKEKLDIEAKYLESKEIADFEKNINAFIAEKCDIIITVGFLLGDA
                     TKAAAEANPNQKFSIVDYGYDPVIPNVLAQAFQTDEAAFLAGYAAAGFTKTGKVGTFG
                     GMQIPTVTIFMDGFARGVRHYNEVHGTSVETLGWDADAQTGLFTNDFSDQQKGSDMA"
     assembly_gap    5431..5530
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            5789..>7280
                     /locus_tag="P5516_08055"
     CDS             5789..>7280
                     /locus_tag="P5516_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016289947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92423.1"
                     /translation="MAPILQLKGITKQFPGVLANDHIDLTLNQGEILALLGENGAGKT
                     TLMNILYGLYQPDEGEILVNGEKITIHSPTDAINAGIGMVHQHFMLIPVFTVTENVML
                     GSEPVKAGDFLDRPKTAAKIMEISSQYKLEVDPNDYVKDLPVGVQQRVEIIKLLYREA
                     NILIFDEPTAVLTPQEADDLFVIMRSLVKQGKSIIFITHKLREVLDVADRIMVIRRGA
                     VVGEADPKQANRNKLAEMMVGREVNLVVEKDIKKPGDVILSVKDLVVTDQFKQVMVDK
                     VSFEVHAGQILGIAGVQGNGQTEFVEAITGLRKSVSGEIMFKGQSILNEPPRKITELG
                     SAHVPEDRQADGLVLPFPVAENLVLCTYYKEPFSRGVVLQYEKILENSEKLVQDFDIR
                     TPSSLTTVGSLSGGNQQKVIIARELSRPIELLVASQPTRGLDVGSIEYIHKRIVQKRD
                     EGCAVLLVSPELDEVIELSDRIAVMYRGKIIALLEAEEVAKETLGLL"
BASE COUNT         1636 a         1912 c         1880 g         1752 t
ORIGIN      
        1 acacccagct gaccgtacgc ggtggggtca tcaggagcaa atgccaccgc gcgcagcatg
       61 ttacgcgccg cggcgaaaaa ttcgccctgc tggacatacg tgttggcaat ggagatgagc
      121 ggaatgggat ccctgacgcc taactggtcg ttgatcgcca cagccttggc aaaagcttcc
      181 aacgccagcc caaaatagta gttctgttcg gttgtgattc ccgtcatgtt ggccagttgg
      241 cggtaattcg cgcccaatga caaatacagg aagttcaggt tcggggtaat ttggatggct
      301 ttcttatact cttcgatcgc ctgagtatag ttcgcgctgg cttcctgcac attgccgttc
      361 acccggtgca catccataag ggtgtcatcc cgctccagcg cctgggtgat gtactgttgc
      421 gcttgtgaat acttcaactg atcaatcaat aattctgcgt aataagccag cgccaacgcg
      481 ttggtattat catattgcag cgcttctgtg gcggcttgtt cccctttgac caggtactcc
      541 tgcgaggctt ctcctgctgc ccctgaagag ccgtaccaat ctaacgcaaa ggctttaacc
      601 gcgtacaggt tactgttttg cgggctgatt gccaaccctt cttcaatggt cgccagcgcc
      661 tcttccaggc gctctctttt gcgcgcatca accgtgattt gcgaagttga gtaaacctgg
      721 atgcgcgcca gctcggccct caggtcggcg tcttcagggt cgatggcaat ggcgtagcgg
      781 taagcctcga tcgctttctc caggttcccc gcctgaaaat gagtttcccc ttcttgcacg
      841 taagaattca ggctacgggt tggcgttggt atgatttgta agatgggggc gatgcgtcca
      901 tcacgcactg cctgaatcac aaacaggccc gccagtatca acatcagcag tgtgaatatc
      961 caaattgggt tggatctttt tcttggatgg ccgaataaag cgcgttttcc gctatagtcg
     1021 atcatagttg cgattgcccg tcatacccaa tggtcagggc gcggcttcca ctgttggggt
     1081 tgtctcagcg ggtgtggccg ttgccgcgat atcagattta ccttcacggg cgaggcgctc
     1141 gcagatattg accatttctt cagcattgaa aacagcgatg tcatcattgc gcagccccat
     1201 ctgaaccgcc tgagaaattt ccagcgcttc gccgcattgc cccaccctcg ccagggcgag
     1261 cccataagtg taataatact gcgccacccg gccataatcc attgggatgc cctcaaccgg
     1321 agcgccatcg gaagataccc cgccacgcac cgcgattcgc agcatatcga tcgcgtcaag
     1381 gtactgcttc gcgctgctgt agatcagtcc caggttgcca tatagtcgcg cattctgtgg
     1441 ctcatccata atcgccgcct cgccgtattg aatagctttc tggtaatcgc cattcaaagc
     1501 gtaagttcga gcgattaaag attcaggggt cggatccctg gggttcaaag cgtttgcccg
     1561 gccaaattca gtgatggcat cgtcatattc ctggatgttg aaataattgc gccccagacc
     1621 aaggtgcaga tcagcgatgt tcggattcaa agctaccgcc gcctcaaatt ctgccacggc
     1681 ttctttaaaa ttcgaggtgt attcaagcac cattccacga ccacggtgtg tttccagggt
     1741 gcccggcgcc agttcctccg cgcggcgcga ggcatttatg gcattgtcca gggtctgcag
     1801 gtctccctgt cctgcctgca gcatataggt gtaaatctcc gccaggtagg catacgccgc
     1861 ggcgtaatca gggttcgcag caatagcctc ttgcaggatg cccgtgccgc gcacatagtc
     1921 tcccgtcaac ccaatcgccc agccgcgcag cgccatcgcc tcgtcgttgt tcgggttaag
     1981 caataaggca ttttccgcat cggtgacggc ttcttcataa ttaccggtat aaatgttcaa
     2041 gcgcgccagt gcaatgaagt tatgcgggtt gcgcggatca gctctgaccg cgtctttgta
     2101 ggctgcgatg gccagagggt atttcccttc agcctccaac cctttcgcgt ctgaggtgat
     2161 ggattcgggc gcgcgggtag gcgtgggcgt aggcagaaaa aactgcggga tagtaggcac
     2221 aatcatgcgg tcaaagtaga tggccgcggc gatcaatacg atcaacagga tcacgcggaa
     2281 tgggttcgac ctgcgtttct ttttctgcat gctccacttg ctgcccttga tatacatgga
     2341 attcatctta ccaagtgaaa ccagcagcgt caagatgacc ggaagccccc cctcgctgtt
     2401 ctttataatc gcaattaagc ttctttggtt acctgggcag ggcttcactt ctcctgtcca
     2461 cttggctccc atacagggac aaatagcaag gttacctatc ggtccgctca ccatcgagct
     2521 catttctttc atcctgatgc gccatttttc ataaagaaaa cggcttgcct gtgttcgcgc
     2581 ttctttgcta gaatgaagcc atgcaattcg atgtctttac tctctttcca ggggttctgc
     2641 ggccttacct cgattcaagc atcctgcagc gcgcccaaca aaatgacctc attaaagtcg
     2701 cgctgcacaa catccgcgac tggacctttg accggcacca tgtgaccgat gatgccccct
     2761 atggtggcgg tggtggtatg gtgatgaaag tggaacctat cttcgctgct gttgagtccg
     2821 tcctgggagc cccaccatct tgcccgctgc tctacctctc tccgcaggga aaacctttca
     2881 ctaccgccat cgcgcgcgag ctggcgcaac aacctcgact ggggttattg tgcggacgat
     2941 acgaaggggt tgacgagcgc gtgattgagc atctggtgac tgaacagatc tcgattgggg
     3001 attatgtgct caccggtggc gagctgccag cgttggtgct catcgacgcg gtatcccgct
     3061 ttattcccgg cgtgctgggt gaccctgacg gagccgagga agatagtttt ggcaatggtc
     3121 tcctggagta cccgcactac acccgcccag aagaattccg tggttggcgc gtaccagaag
     3181 tgcttatctc tggcaatcac gccctggtcg ccaaatggcg ccgagaacaa tccctgttgc
     3241 gcacccaaaa gcagcgtcca gacctgttaa atggtgaatc caaggttcaa tctaccacaa
     3301 aaacaaagga gtaagagatg gagaaaaaga agtttccatg gggtcgtacg tttctggtag
     3361 ggtttggttt ttttggcatc agcattttat ggccaatctt caatcaatgg gtgccgctga
     3421 tcttgcaggc gggtaatccc ggatttaaaa ccaacatccc caattttgtc ggttttggca
     3481 ttggcccagc gctcgccatg ttcatcatga cctgggacaa tatcgtcaac atcttcgtac
     3541 agccatgggt ggggaatcgc agcgatcaca cctggaatcg tttcggtcgg cgaaaaccgt
     3601 ggattctact gggtttaccc atcgcgctcg tgggctttat ttcaattccg ttggcgcaat
     3661 cagtgctggc ggtggcggtg ttcatcatgg tcaccaatgt cggcatggca cttttccgct
     3721 ctccggttgt ttcatggctg ggcgacctgt tcctgccaga gcagcgcagc aaggcgaacg
     3781 gcgtcatcaa cctgatgggc ggtgccgccg gtgtcctatc gtacgtactg ggagggctga
     3841 tcttcaacgc ctttggcagg atgggtccct tcgtcgccgg cgcggtcgcc atgaccgtgg
     3901 cgttggccgc ggtgttgatc tgggtaaaag aacccaaaga acgcctggga aagcacaccg
     3961 aagatgagga cagcagcggc gcggtgaagg gattggtgcc caaccttaaa gcggtcttca
     4021 gcaaccagga taaatcaacg cttttcattc tactggccat cttctgctgg ttcaccggct
     4081 ttaacgccat tgaaacgggg ttgtcttcct tcgcggtctt cgatctgggc attgcccctg
     4141 gcaaggcttc gatcgtggct tcaattgcca atctttcttt cctggctttc tgcattcctt
     4201 caggaattct cgctggcagg ttgggtcgta agaggaccat cctctatggc gtgttcgcgc
     4261 tcacgctcat ctttctcatc agtttcttcc tggtgaaagg gctcatctcc ttcattatca
     4321 tcctggtgct ggtagggttc ttctggtcct gggtcaacgt caacagcctc ccgctggtgt
     4381 acgaccatgg agatgaaaaa cgcattggcg cgtataccgg tctttattat ttcttctcac
     4441 aaaccgcagc cgtgctcgga ccaaccctgg gcgggctgct ggttgaaaga atacacttca
     4501 actacaaagt gctctttatc tttggcgcgg tctttctgtt cctggcgttc ttatccatgc
     4561 tgaaggtaaa agaccgcgcg cacgaagaaa aaatgaagct gggcgcgggt attggcgcat
     4621 cctcatccgc cagcgaataa acggtcctga atgaaaaagt ctctccatct gggatttttc
     4681 atggctggga taacttgcat tgaagggcaa atatcggtat actcaattaa tagacggggc
     4741 aatcccccgc acatccaaca accctgcagg aggagaaaaa atgtctaaaa aagtaatgct
     4801 cgtcatgtca gtgcttctaa ttgccgtttt tgtgttgagc gcgtgcgcga aaacagccgg
     4861 accggcaaaa tttaaagcct gcgaagtcac cgataccggt ggcgtggatg acaaatcctt
     4921 caacgcgacc gcatggaagg gcgcggtaga tgccaaagaa aagctggata ttgaagccaa
     4981 gtatttggaa tcaaaagaaa tcgctgattt cgagaagaac atcaacgcct tcatcgctga
     5041 gaaatgcgac atcatcatca ccgttggttt cctactgggc gatgccacca aggcagcggc
     5101 tgaggccaat cccaatcaga agttctccat tgttgattat ggctatgatc ccgtaatccc
     5161 caatgtcctg gcccaggcat tccagactga tgaagccgca ttcctggctg gttacgccgc
     5221 ggctggtttc accaagaccg gtaaagtcgg caccttcggt ggaatgcaga ttccaaccgt
     5281 gaccatcttc atggatggct tcgcgcgcgg cgtgcgccac tacaatgaag tgcacggtac
     5341 ttctgtagaa accctcggct gggatgcaga cgcacagacc gggctcttca ccaatgactt
     5401 cagcgaccag cagaaaggca gcgatatggc nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5461 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     5521 nnnnnnnnnn tctggccaat ggtggtgtag gtatcgcacc gttccataac ttcgacggtc
     5581 aggtttccgc tgagctgaaa gccgaactcg aagggattaa agctggcatc ctctccggcg
     5641 aaatccagat gaaccccgct ttcaagcagt aattcgcgca catgtgagaa aaagctggag
     5701 ttattcgctc cagctttttt tttaattttt gagtttgaga cgcctgtgat acaataatca
     5761 aaatcccctc tgaacaagtg gtgtatacat ggctccgatt cttcaactca aaggaattac
     5821 aaaacagttt cccggcgttc tggcaaatga ccacatcgac ctcactttga atcagggcga
     5881 gatattggcg ttgctgggtg aaaacggcgc tggtaaaacc accttgatga acattctcta
     5941 cgggttgtat cagccagatg aaggcgagat tttggtgaat ggcgaaaaga taaccattca
     6001 ttcgccaact gatgccatca atgctggtat cggtatggtt caccagcact ttatgctcat
     6061 accggtgttc actgtaactg aaaacgtgat gttgggttct gagccagtga aagccggcga
     6121 tttccttgac cggcccaaga cagccgcgaa gattatggaa atctccagtc aatacaaact
     6181 ggaggttgac cctaatgatt acgtcaaaga cctgcccgtc ggcgtgcaac agcgcgtaga
     6241 aatcatcaag ctcctgtacc gcgaagccaa tatcctcatc tttgacgagc ccaccgctgt
     6301 gctgaccccc caggaagcgg acgacctctt tgtcatcatg cgttccctgg taaaacaggg
     6361 caagtccatc atctttatca cccataaatt acgcgaagtt ttagacgtgg cagaccgtat
     6421 catggtcatc cgccgcggcg cggtggtggg agaggcggac cccaaacaag cgaaccgcaa
     6481 caaactggca gaaatgatgg ttgggcgcga agtcaacctg gtggtggaaa aagatatcaa
     6541 aaaacctggt gatgtgatac tttcagtaaa agacctggtg gtgactgacc agttcaaaca
     6601 ggtgatggtg gataaagtat cctttgaggt tcacgccggt cagattctgg gcatcgccgg
     6661 ggtgcagggg aatggtcaga ccgagtttgt agaagccatc accggactga gaaaatcagt
     6721 atccggcgag attatgttta aagggcagag tattctgaat gaacctccga gaaaaatcac
     6781 cgaactgggc tctgcccatg taccagaaga ccgccaggct gatggattgg tgctgccctt
     6841 ccctgtagcg gaaaacctgg tgctgtgcac ctattataaa gagccttttt cgcgcggcgt
     6901 ggtgctgcaa tacgaaaaaa tccttgaaaa ttccgaaaag ctggtgcaag acttcgacat
     6961 ccgcaccccc agctctctaa ccactgtcgg cagtctttcg gggggtaacc agcaaaaagt
     7021 gatcatcgcc cgtgagcttt cgcgccccat cgaattgctg gtcgcttctc aaccaacccg
     7081 tggtctggac gtgggttcca ttgagtacat ccacaagcgc atcgtacaga aaagagatga
     7141 aggctgcgcg gtgctgctgg tttcacctga actcgatgaa gtgattgaac tttcggaccg
     7201 catcgcggtg atgtaccgtg gcaaaattat cgccctcctt gaagccgagg aagtagccaa
     7261 agaaacttta gggttgttga
//
LOCUS       DAOTLW010000063         6813 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_10649_length_6813_cov_9.834566, whole genome shotgun sequence.
ACCESSION   DAOTLW010000063 DAOTLW010000000
VERSION     DAOTLW010000063.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6813)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6813)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6813
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10649_length_6813_cov_9.834566"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1381
                     /locus_tag="P5516_08060"
     CDS             <1..1381
                     /locus_tag="P5516_08060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HRT92424.1"
                     /translation="SIPVEKRPGVEVSGSTLNKYGLLKIKALRVGKDTVLSQIIRLVE
                     HAQETKAPVQKLADEISKVFVPVVIGLAVLTFGIWMIVNPPVDNPHGVTGITRALINM
                     VAVLVIACPCAMGLATPTAIMVGTGRGAEMGILFRSGEALERAGKTEVVVLDKTGTIT
                     RGQPQVTDIVSVHEGISKEQLMQTAASVEKGSEHPLGEALIAAATEMGLNLTEPANFL
                     AVPGKGVSAEINAERVLVGTLDFLQENGFDISPIMPAAEEYRRQGKTIMAVAQNTEVI
                     GVVAVADTLRTGSKEAVKELKEMGMQVIMLTGDNLETAREIGQQAGIDQVIAGVLPEG
                     KINEIRRLQESGKVVAMVGDGINDAPSLAQADLGVAIGSGTDVAVAAAPVTLIGSDLR
                     GVVKAIALSRKTLKTIRQNLFWAFFYNAILIPAAALGVLNPMLAAGAMAFSSVFVVTN
                     SLRLKKASI"
     gene            1474..3486
                     /gene="tkt"
                     /locus_tag="P5516_08065"
     CDS             1474..3486
                     /gene="tkt"
                     /locus_tag="P5516_08065"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HRT92425.1"
                     /translation="MMTTIEQKAINTIRFLSADGVQKANSGHPGLPMGTAAIAYTIWA
                     KHLKYDTSNPQWWDRDRFVLSGGHGSMLLYSLLHLAGFDLSLDDLKNFRQWGSKTPGH
                     PEFGHTMGVETTTGPLGQGFSNGIGMALAEAMLAATYNKPGQKLFDHYVYAIVTDGDL
                     MEGISSEAASLAGHWQLGKLIYCYDDNRISIDGSTDLTFTEDRGKRFEAYGWQVLHVD
                     DGNDVKKIDQALKEAKSDPRPSLIICRTHIGFGLPSKQDTAKAHGEPPGIDELQNAKR
                     KLGWPIAPDFYIPEEVRSHFNQVTARKNEQAMKWREKAAQYAVAYPNEWQQLQERMTG
                     EIPDTEHFELPEFPTDQKGMATRSASGQVLNALADILPQLVGGSADLTPSNNTWIKNS
                     AAFQKDNHAGRYLHFGVREHGMGGIVNGLAYHGGFIPFGATFLVFSDYMRGSIRLSAL
                     SKLQTIWIMTHDSIGLGEDGPTHQPVEHLAALRAIPNLFVIRPADANETREAWRVAVQ
                     RQDGPTLLALTRQNVPTIDRKIYAGAEGLHKGAYILADLGQGKVPEIILMASGSEVSL
                     IIAAGIHLANQNHRVRLVSFPCWELFEKASQQYQNAVLPTQVKTRLAVEMGVGLGWKK
                     WVGDEGEVISIETFGASAPYQKLLEEYGFSVANVIAKAKKLLKKKR"
     gene            3489..3935
                     /gene="rpiB"
                     /locus_tag="P5516_08070"
     CDS             3489..3935
                     /gene="rpiB"
                     /locus_tag="P5516_08070"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HRT92426.1"
                     /translation="MRIAIAADHAGFPIKDEVINYVQSAGHDVIDLGTYNQEPVDYPD
                     YAMKVGRAIQENQSDRGIVICGSGVGATIAANKIKGVYACVCHDTYSAHQGVEHDNMN
                     VLCLGSRILGIELVKEIVSAFLAAEFIHDGRFLRRFEKICALEKAQ"
     gene            3960..6749
                     /locus_tag="P5516_08075"
     CDS             3960..6749
                     /locus_tag="P5516_08075"
                     /EC_number="2.2.1.2"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF007080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional transaldolase/phosoglucose
                     isomerase"
                     /protein_id="HRT92427.1"
                     /translation="MNIIKEIHKAGQSFWYDNIERAKLLDGSLAELIRKGAISGITSN
                     PSIFQKAISTSFDYDITLKPMAWSGLDSEQIFWQMAIEDIQKAAQLFLPVYESSQGMD
                     GYVSLEVNPLLAHDTEGTVKEARALSERVKTPNLMIKIPATKEGIPAIRQCTSEGLNI
                     NVTLIFSNDRYKEVMDAYLSGLEDRMRRGEPIGRVTSVASFFVSRIDTLIDKLLNEKL
                     HNGEIKAQLYESLAGRAAIANARLAYRLFNEVFNSQRFLSLKANGARLQRPLWASTST
                     KNPAYRDVLYVEELIAPDTINTVPPATLDAFLEHGSVAVRIFDSANETDSLFERLNKL
                     GISIDQVTEQLEIEGVKAFTEAYSSLLDAVDKRRLSAIKEISSLASPVKTSVGELKRI
                     NFVSRLYQKDPALWTAEPEGQAEARNRMNWLETPFSNQEKEPAYAGIGALLRAEGFTH
                     AVLLGMGGSSLAPEVLSRIIAAEEQDPLKGLALSILDSTDPHQVKLIQEQNPALNTLY
                     IVASKSGTTGEINALFDYFWNRTVLAGCKNPGAHFLAITDPDTKLDQLAKEKGFRAVY
                     HADPQVGGRYSALTAFGLVPAAIMGLDLKRLNKSAMNIAQFCRPGQPIEANPGVVLGA
                     ILGTAAKPGKDKVTLITDNNWNAFGDWLEQLIAESSGKEGKGMVPVTAEPVMNVEDYS
                     PDRLFVYLEKDHSQADFAALLKNAGHPVVTLQVTENDDLGGQFYLWEVAVATACSIIG
                     VNAFDQPDVQDAKLRTLAGLAAYRETGSLPEMSPAARFTRASVSGFVQEKAEENEKLI
                     DYIERMVSTCEKTIEYFAINAFLPKNRKNEQILQELRTRIGQRFQIGTTLGFGPRFLH
                     STGQLHKGGPNKGFFLVITAARQDDLEIPGQGVKFGVFQRAQAIGDLSALQAKDRCAL
                     WVDLDEPDPEILLME"
BASE COUNT         1875 a         1569 c         1787 g         1582 t
ORIGIN      
        1 atccataccg gtagaaaaaa ggcctggcgt tgaagtcagc gggtcaacgt tgaataaata
       61 cgggctcctc aagataaaag cacttcgcgt gggaaaagat acggtgcttt cacaaattat
      121 ccgcctggtc gaacacgccc aggaaacgaa agctccggtt caaaaactcg cagatgaaat
      181 ttcaaaggtg tttgtcccgg tggtgattgg tttggctgtg ctgacgtttg gcatatggat
      241 gatagttaat ccgccggtag acaacccgca tggggtaacc ggcatcaccc gggcgctgat
      301 caatatggtc gccgtgttgg taattgcctg tccttgcgcc atggggctgg caactcccac
      361 ggctattatg gtaggaaccg ggcgtggcgc agagatgggg atcttattca ggtcaggtga
      421 agcgcttgaa agggctggta aaacagaggt ggtcgttttg gataagactg gcaccatcac
      481 caggggacag ccacaagtca ccgacattgt ttctgtgcat gagggaatct caaaagagca
      541 actgatgcaa acggctgcca gcgtggaaaa aggcagcgaa cacccattag gagaagcact
      601 tattgctgcc gcaacagaga tgggattgaa tctgacagaa cctgccaatt ttctggctgt
      661 acccgggaag ggtgtatcgg ctgagatcaa cgctgagagg gtgcttgttg gaacgctgga
      721 ttttttacag gaaaatggtt ttgatatctc gccgatcatg cctgctgccg aagagtatcg
      781 gcgccaaggc aaaaccatta tggcagtggc acaaaacaca gaagtgattg gggtagtcgc
      841 ggtagcagat acgctgagga ctggctcaaa ggaagcagtc aaagaattaa aagagatggg
      901 catgcaggta atcatgttga ccggggacaa cttggaaacg gccagagaaa tcggtcagca
      961 ggctggaatt gaccaggtga ttgcgggcgt cctgccggaa gggaagatca acgaaattcg
     1021 ccggctgcag gaaagcggaa aagtggtcgc catggttggc gacgggatca atgacgcgcc
     1081 ttcactcgca caggcagacc tgggtgtggc catcggtagc ggcacggatg tcgctgttgc
     1141 cgctgcgcct gttacgttaa tcggcagcga tttaagaggt gtagtaaaag ccatcgcgct
     1201 ttcgcgtaag accttaaaga ccatccgcca aaacctcttc tgggcttttt tctataatgc
     1261 cattctcatt cctgcagcgg ctcttggggt gctcaaccct atgctggctg ccggagcgat
     1321 ggcattcagc agcgtctttg tggtaaccaa cagcctccga ttaaaaaaag ccagtattta
     1381 aacaaatgat tctgatcagc gctttttagc ggaacacacg cattcagcct atctatttca
     1441 tgatggtaaa atacaatgac gagtcacgga gggatgatga caaccattga gcagaaagcc
     1501 atcaatacca tcaggttctt atcagcagac ggcgtacaaa aagccaattc aggacaccct
     1561 ggtcttccta tgggaactgc ggctatcgcg tatacgatct gggcaaagca tcttaagtac
     1621 gataccagca acccccaatg gtgggatcgc gatcggtttg tcctgtctgg cggacacggt
     1681 tccatgctgc tttatagcct gcttcacctg gctggatttg atctatcgct ggatgatctg
     1741 aaaaatttca ggcagtgggg ttctaaaact cccgggcacc ctgagtttgg gcacacgatg
     1801 ggcgtggaaa ccaccacggg cccattgggg caggggtttt ccaacggaat aggtatggcg
     1861 ctcgcagaag ccatgcttgc ggctacatat aacaaacctg ggcagaagtt gttcgaccat
     1921 tatgtttacg ccattgtcac cgatggcgac ctgatggaag gaataagctc agaagccgct
     1981 tctttagcag gtcactggca gttaggaaag ctcatctatt gttacgatga taatcgtatc
     2041 agcatcgatg gatctacgga tctcacattc accgaagatc gcggtaaaag attcgaagca
     2101 tacggctggc aggttttaca tgtcgatgac ggaaatgatg ttaaaaagat agaccaggcg
     2161 cttaaagagg cgaaaagcga tccacgtcct tcgttaatca tctgcagaac gcacatcgga
     2221 tttggtttac cttcaaaaca ggataccgcg aaggcgcatg gtgaaccgcc tgggatcgat
     2281 gaacttcaaa acgccaaaag aaaacttggc tggccaattg cgcctgattt ttacataccc
     2341 gaagaagtgc gcagccattt caatcaagtc actgcgcgga aaaacgaaca ggcaatgaaa
     2401 tggcgtgaga aggcggcaca atacgcagtc gcatacccaa acgagtggca gcaacttcaa
     2461 gagcggatga ctggagaaat acccgataca gaacatttcg aactgccaga attcccaact
     2521 gatcaaaagg ggatggccac caggtcggct tcaggacagg tgttgaacgc gcttgctgac
     2581 atccttccac agcttgttgg cggttccgcg gacctgaccc catctaacaa cacctggatc
     2641 aagaacagcg cggccttcca aaaagacaat cacgcaggac gctatttgca ttttggtgta
     2701 agggaacatg ggatgggagg gattgtaaac ggactcgcct atcatggcgg ttttatccct
     2761 tttggggcaa ctttcctggt tttctcagac tacatgcgcg gttccatacg cctttctgcg
     2821 ctttccaagc tgcagaccat ctggattatg acccatgata gtattggttt gggtgaagac
     2881 ggacccaccc atcaaccagt ggaacacctg gccgcattgc gcgcaatccc caacctgttt
     2941 gttatccgcc ctgctgatgc caatgaaaca agagaagcct ggcgtgtagc ggttcaaagg
     3001 caagatggcc ctacgctatt ggcgttgacc cgccagaatg tgcctacaat tgatcggaag
     3061 atatacgcag gcgctgaagg attacataaa ggggcctaca tcctggctga ccttggccaa
     3121 ggcaaggtgc ccgagatcat cttaatggct tcaggctcag aagtgagcct gatcatcgcc
     3181 gccggtattc acctggccaa tcaaaatcac cgcgtacggt tggtttcttt cccctgttgg
     3241 gaattgttcg aaaaagccag ccagcagtat caaaacgctg tcttaccaac tcaagtcaag
     3301 acccgactgg cggttgaaat gggtgttggg ttgggatgga aaaaatgggt gggagacgag
     3361 ggcgaggtaa tcagtattga gacttttggc gcttctgctc cttaccaaaa gttgttggaa
     3421 gagtacggtt tttctgtcgc caatgtaatc gcgaaagcca aaaaattact taagaaaaag
     3481 aggtagaaat gcgcatcgcc atcgccgcgg accacgcggg ttttccaatc aaagatgagg
     3541 tgatcaatta tgttcagtcc gccgggcacg atgtaattga cctgggaact tacaaccagg
     3601 aacctgttga ttaccctgat tatgccatga aagttggcag agcgattcag gagaatcagt
     3661 ctgacagggg tatagttatc tgcggatcag gcgttggcgc tactatcgcc gcgaacaaaa
     3721 tcaaaggcgt ctacgcatgt gtatgtcacg acacgtattc tgctcaccag ggggtggaac
     3781 acgataatat gaacgtgtta tgcctgggca gccggatttt gggaattgaa ctagtgaaag
     3841 aaatcgtgtc agccttttta gcagcagaat tcatccatga cggcagattt ctgagacgtt
     3901 tcgaaaaaat atgcgcgctt gaaaaggcgc agtgagcgag tggattgagg tccaaccgaa
     3961 tgaatatcat caaagaaatt cacaaagcag ggcaatcttt ttggtacgac aatattgaaa
     4021 gagcaaaatt attagatgga agtctggctg aactgatccg caaaggggct ataagcggca
     4081 tcacttctaa tccaagtatt tttcagaaag ccatctcaac ctcctttgat tacgatataa
     4141 cactcaaacc catggcctgg tcgggattag acagtgaaca gatattttgg caaatggcca
     4201 ttgaagacat ccaaaaagcg gctcaacttt tcctgccagt gtatgagagt tcccaaggga
     4261 tggatgggta tgtcagttta gaggttaatc cactcctcgc gcatgacaca gagggcacag
     4321 ttaaagaagc ccgtgcatta tcggaacggg taaaaacacc caacttgatg atcaagatac
     4381 ccgccacgaa ggagggtatc ccagctattc gacaatgcac cagtgaaggg cttaatatca
     4441 atgtaaccct gattttttca aatgaccgtt ataaggaagt aatggatgcc tatctttcgg
     4501 gcctggaaga taggatgcga aggggagaac ctattgggcg cgtcacttcg gttgcttcct
     4561 tcttcgtctc tcgcattgac acgcttattg ataaattatt gaacgaaaaa ctccacaatg
     4621 gagaaattaa agcccaatta tatgaatcgc tagcgggcag ggctgccatt gccaacgcgc
     4681 gtttggctta taggctcttc aacgaggttt tcaactcgca acgtttctta tcacttaaag
     4741 ccaatggtgc caggctgcaa agaccgttat gggcatccac gagcaccaag aaccctgcct
     4801 acagagacgt cttgtatgtg gaggagttga tcgcgccgga tacgataaac actgttcctc
     4861 ctgccacgct tgacgccttt ctggaacatg gctcagtggc cgtgagaata tttgacagtg
     4921 ccaatgaaac ggattcatta ttcgaacgcc tgaacaagtt gggcatatcc attgatcaag
     4981 taaccgaaca actcgaaatt gagggcgtaa aggcttttac agaagcttat tcttccttac
     5041 ttgatgctgt tgacaaaaga aggctttccg cgatcaagga aatatcttca ctggcatcac
     5101 cggtaaaaac aagtgttggt gaactcaaaa gaatcaattt tgtgtcaagg ttgtaccaga
     5161 aagaccctgc actttggacg gctgaacctg aaggccaggc tgaagccaga aaccgcatga
     5221 attggcttga aacacctttt tccaaccagg aaaaagaacc tgcctacgcg ggcattggtg
     5281 cgctgcttag ggctgaaggt ttcacacatg cggtccttct gggtatgggc gggtcatccc
     5341 ttgcgccaga agtcctgagc cgaattatcg ctgctgaaga gcaagacccg ttaaagggct
     5401 tagcgctgag cattctggac tccactgacc cccatcaagt aaaacttatt caagaacaaa
     5461 acccagcctt aaatacgctc tacatcgttg ccagcaagtc tggaaccact ggtgaaataa
     5521 acgcattatt cgattatttt tggaacagaa ctgtcttggc ggggtgcaag aatcccggcg
     5581 cgcatttttt ggcgatcact gaccctgata caaaattaga tcaattagcg aaagaaaaag
     5641 gattccgtgc ggtataccat gctgaccccc aggtgggcgg acggtattca gcgcttacgg
     5701 cttttggctt ggttcctgct gccatcatgg ggcttgatct gaaaagacta aataaaagcg
     5761 cgatgaatat cgctcagttt tgcagaccag ggcagccaat agaggcaaat cccggcgttg
     5821 ttttgggtgc catattggga accgcggcga agcccggcaa agacaaggtg acccttatta
     5881 cagacaataa ctggaatgcc ttcggcgatt ggctggaaca gttgattgcg gagagtagcg
     5941 gcaaagaagg caaagggatg gtgcctgtta ccgctgaacc tgttatgaat gtggaggatt
     6001 attcgccaga taggcttttc gtgtatcttg aaaaggacca ctctcaggca gatttcgctg
     6061 cattattaaa aaatgccggc cacccagttg ttacattgca ggttacagag aatgatgacc
     6121 tgggaggtca gttctaccta tgggaggtgg ctgttgcgac cgcttgcagc attatcggcg
     6181 tcaatgcatt tgaccagcca gatgtacagg atgcaaaatt acgcaccctg gcaggtttgg
     6241 cagcctaccg tgaaacaggt tctttacccg agatgagccc agccgcgcga ttcaccagag
     6301 ccagcgtctc tggttttgtc caagagaaag cggaagaaaa cgaaaagctg attgactata
     6361 tcgaacgaat ggtctcgacc tgcgaaaaaa ccattgaata cttcgcgatc aacgcgtttc
     6421 taccaaagaa caggaaaaac gagcaaatct tacaagagtt acgaacgaga atcggtcaga
     6481 ggtttcagat aggcactacg ctgggttttg gccctcgctt tttacattca accgggcaat
     6541 tgcataaagg cggaccaaac aaagggtttt tccttgtaat taccgccgcg agacaggatg
     6601 accttgaaat cccggggcag ggagtgaaat tcggcgtgtt ccagcgcgcg caggcgattg
     6661 gcgacctgtc ggcgctgcag gcgaaagacc ggtgcgcatt atgggtcgat ctagatgaac
     6721 ctgacccaga aattctttta atggagtaaa aaaaggacac gtatcttgag cgggtgaatg
     6781 ttcaacccgc tttttttaat tcaaattcca ata
//
LOCUS       DAOTLW010000064         6721 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_10798_length_6721_cov_11.606961, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000064 DAOTLW010000000
VERSION     DAOTLW010000064.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6721)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6721
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_10798_length_6721_cov_11.606961"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(212..1216)
                     /locus_tag="P5516_08080"
     CDS             complement(212..1216)
                     /locus_tag="P5516_08080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92428.1"
                     /translation="MQELIEHLNSINREQLLLLEGMSTELLERLLSSRPFSSREEILE
                     VEGVTPDLLSAWENNLHPERKTENDSSEPAVKKGRGVGRVILRVLTALLILAALAAAA
                     YYGIPYFREKILKPLESNTSRVGELAATQAADTSRFEADLGALQEEFTNLQGQVSALQ
                     TQAAAASKSIDSHSASLNELSEVQATLRAEMAQQNNALLANMDEQLTLTRALELLSRS
                     RLYLSQNNYGMARADVLAARNLLLPLVGSVSAQKADGLRVVIDRLDMALGNLPAYPVI
                     AVFDVDTAWQMLVDGLPSIPEPAVTPMLITPTPQPTTLPESTTPVPLQTQPVAPSATP
                     "
     gene            1400..2068
                     /locus_tag="P5516_08085"
     CDS             1400..2068
                     /locus_tag="P5516_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92429.1"
                     /translation="MDDLFVSAIVVVVTAVIVAAIFFITGKKKREKEAALLQLAQRKG
                     WRYEKVNQGKHTGFILRAPEWTLESLVYSDYRTSEVGSSTVAYHNRWLTDRVSSTHGL
                     VMIGPKLPHAQLGALNSLMLRKALQLMLGDEADQAASLVEMQVGRSSFSERYSVWAVN
                     QEGVEQVLTYNLENALLRWKGKELPVLKFSAAGAQISTRHGRLDSVEQVQEMVDLGMA
                     VLAG"
     gene            2261..3298
                     /locus_tag="P5516_08090"
     CDS             2261..3298
                     /locus_tag="P5516_08090"
                     /inference="COORDINATES: protein motif:HMM:NF024778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HRT92430.1"
                     /translation="MAALIAALITGFTTGGLSCMAVQGGLLASSLAQLIEKDLLGKRG
                     GRDQWNIGLPIFLFLISKLAAYTILGGLLGGLGKLFQLDPLSQAVLQFIIAFFIIGNA
                     LRMLNVHPLFRYFTFEPPAFITRLIRKKTRQAASFTTPIMLGAMTVLIPCGVTQTMMA
                     AAIATGDVLSGAALMFAFTLGASPVFFLLSYFATKLGALTEKYFTRIAAVVLIVFGLL
                     AVNSGLNLLGSRYSLSRLAENAVSAVEIRHQGQASATPGTDLVLNVVDRGYRPKRLHA
                     PAGVPLTLTLVTKEVHSCSRAFLIPALDYGVILLATGEEKVQIPPQEPGTILPFTCSM
                     GMYSGEIIFDL"
     gene            3312..3521
                     /locus_tag="P5516_08095"
     CDS             3312..3521
                     /locus_tag="P5516_08095"
                     /inference="COORDINATES: protein motif:HMM:NF012621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HRT92431.1"
                     /translation="MKRVELRVMDMHCTSCALRLQGLEDLLAGVIRVEASYRKGKMTV
                     TFDESKVTVKDLVEGARKLGYTAVE"
     gene            complement(3549..4241)
                     /locus_tag="P5516_08100"
     CDS             complement(3549..4241)
                     /locus_tag="P5516_08100"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0016018 - cyclosporin A binding [Evidence
                     IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HRT92432.1"
                     /translation="MSDKTSRSKAVRQKSKKRSREYSDLTLILIVLLVLAVAIVGGYV
                     IKSRQAARVSPTAAVMNYKQYSAAPPMTIDPAKQYLATVKMAKGGEFVIQLYPDKAPV
                     TVNSFVFLAREGYFDGVTFHRVLAGFMAQTGDPSGTGSGGPGYQFENEDSDLVFDKAG
                     VVAMANAGRDTNGSQFFITYAPVEHLNGDYTIFGQVISGMEVVKDITRRDPQTNPKFS
                     GDVIESITITEQ"
     gene            complement(4260..4523)
                     /locus_tag="P5516_08105"
     CDS             complement(4260..4523)
                     /locus_tag="P5516_08105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92433.1"
                     /translation="MARVIPIAQRISKARALMQKARDLPLPAEQGWLDFSYAANVRDI
                     LRQARDLLKFIQFMPNASAEAKREVEDLLLEINRAEKEILHKS"
     gene            complement(4598..5548)
                     /locus_tag="P5516_08110"
     CDS             complement(4598..5548)
                     /locus_tag="P5516_08110"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013646.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HRT92434.1"
                     /translation="MLRTEVLIVGGGPAGSACAWQLKQHGVEFILLDRAEFPRPKVCA
                     GWVTPRVLRDIQLDPAAYPGNFSVFNQFLIELKGLRFTLQTRQYAIRRVEFDQWLINR
                     VRAQVLTHQVKEIRQEGEHYIVDEEYSARYLIGAGGTNCPVKRALFEPVGQPVRGSLI
                     VAQEEEFIYPGASRLCHLWFLQQGLPGYAWYFPKSENVVNVGVGGSAARLKERGDTLR
                     HHWELLTERLYAYGLVRDHPFNPLGHSYYLRAAQPLLRKGNALLIGDALGVSTLDMGE
                     GIGPSIQSGINAAMAIINHTPYTLDGISRYSFPSLLRLQR"
     gene            complement(5555..>6721)
                     /gene="cfa"
                     /locus_tag="P5516_08115"
     CDS             complement(5555..>6721)
                     /gene="cfa"
                     /locus_tag="P5516_08115"
                     /EC_number="2.1.1.79"
                     /inference="COORDINATES: protein motif:HMM:NF008686.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane fatty acyl phospholipid synthase"
                     /protein_id="HRT92435.1"
                     /translation="LLNNSGVFTRITVKEVMMASAESVVRELLDLAQVKVNGPDPWDI
                     QVHNPAFYDRVLRDGTLGLGESYMEGWWDCPAIDQFITRALLARLDEKVKNESRFLLQ
                     ALRSRLFNRQSRGRAFEVGIKHYDLGNDLYQAMLDKRLVYTCGYWKDAKNLDDAQEAK
                     LDLVCRKIGAHPGMRILELGCGWGSFAKFAAEKYGVEVLGVTVSKEQVALGMELCKGL
                     PVELRLQDYREVSGTYDAVISIGVMEHVGYKNYRDYMLVVDRCLKPEGIGFFHTIGSN
                     RTITRGEPWTDRYIFPNGVLPSISQLGRAMEELFVVEDWHNFGPYYDPTLMAWSANFE
                     KAWPSLQPRYGDVFYRMWRYYLLTSAGGFRSRHQQLWQVVFTRQGNPQPACRLS"
BASE COUNT         1505 a         1775 c         1865 g         1576 t
ORIGIN      
        1 actcccacca ccgaccagaa ttttcgtgga aattcttgcg ctatcttctt aaaatccata
       61 ttcaacattt acgagcgtct ttgataaagg aaaagaatat caatcatatc acttaatcac
      121 aagcgccact tccacttaaa ccaattgagg tagtgacgtg aaaaattcag cctttacggc
      181 tggcggtatg cgtcgtcagc gggcgcttca gttatggggt ggcgctggga gccactggtt
      241 gtgtctgcag tggcaccggc gttgtgctct ccggcagggt cgttggttgc ggggtcggcg
      301 tgatgagcat gggggtcacc gcgggttcag gaatagaagg caagccgtcc accaacatct
      361 gccacgcggt atccacatcg aacacggcaa tcacagggta agccgggaga ttgcccaggg
      421 ccatgtccaa ccgatcaatg accacgcgca gaccatcggc tttttgcgcg gatacgcttc
      481 ccacaagcgg caagagcagg ttgcgcgcgg ctaacacatc ggcgcgcgcc atgccgtagt
      541 tgttctgcga caggtacaac cggctgcgcg acagcagttc cagcgcccgt gtcagcgtca
      601 attgttcatc catattcgcc agcagcgcat tattttgctg cgccatctcg gcgcgcagcg
      661 ttgcctgtac ctctgaaagc tcgttcaggc tggcggagtg cgaatcaatg gatttactgg
      721 ctgctgctgc ctgtgtttgc aaagcggaga cctgtccctg caggttggtg aattcctcct
      781 gcagcgcgcc gaggtccgct tcaaagcggc tggtatccgc agcctgggtg gcagccagtt
      841 cgccaacccg gctggtgttg gattcaagcg gtttcaggat cttctcgcgg aagtaaggaa
      901 taccgtaata ggctgccgcg gcgagcgccg ccaggatgag gagcgcagtc aatacacgca
      961 aaatcactct ccctacgcct ctgcctttct tcaccgccgg ttcagaggaa tcgttttcgg
     1021 tctttcgctc gggatgcagg ttattctccc aggcagagag caggtcaggg gtaacaccct
     1081 caacctcgag gatttcttct ctggatgaga agggtcttga agacagcaag cgctccagca
     1141 gttcagtgct cataccttct aaaagcagaa gctgttcgcg atttatcgag ttgaggtgct
     1201 ctatcagttc ctgcatgttt ctatcctccg cttgtaaatt atccctcatt ataaccttat
     1261 ccccgcggct tgcttgagac aatccagaga cacctggcag gtacgcgcac agtgtttgaa
     1321 cctgattgtt caacctacgc tctttctata taatatgaag acatgatgtc catgcttatc
     1381 aacttaccag gagagaaaaa tggatgatct tttcgtttca gcgatagtcg ttgtggtcac
     1441 cgcggtgatc gtggctgcca tcttcttcat caccgggaag aagaaaagag agaaagaagc
     1501 cgctctttta caactggccc agcgcaaggg ttggcgctat gagaaagtaa accagggtaa
     1561 acatacgggg ttcatcctgc gggcacctga atggacgctg gagtcgctgg tctattctga
     1621 ctatcgaacc agtgaggtag gttcgtcgac cgtggcgtat cataaccgct ggttgacaga
     1681 ccgtgtatct tccacgcacg ggctggtgat gattgggcct aaattacccc acgcacaact
     1741 gggagcgctc aatagtctga tgctgcgcaa agccttgcaa ctgatgctgg gagacgaagc
     1801 agaccaggcc gcgagcctgg tagagatgca agtgggacgc agctcgttca gcgaacgtta
     1861 ttccgtgtgg gctgtcaacc aggaaggcgt tgagcaggtg ttaacttaca acctggaaaa
     1921 cgcgttgctg cgctggaaag gcaaggaact gccagtactc aagttttcag cggctggagc
     1981 tcaaatcagc acgcgccacg gacggttgga tagcgtggaa caggtccaag aaatggtaga
     2041 cctgggcatg gcggtgctgg caggttagca gatcatccat gcaggtgagc aggagtatcc
     2101 cgccggtaac ccagggatag ggaaacttac attggattat ccctttgcct gacattgaaa
     2161 aaagcccaaa agaagacagg tctaatttcg gattaaatgg atataatatc tcgaaattag
     2221 agatggcatc tttttatatg aattgagctc tgggtaactt atggcggctt tgatcgcggc
     2281 gttgatcacc gggtttacga caggcggact tagctgcatg gcggtgcagg gtggtttgtt
     2341 ggccagctca ctggcgcagc tgatcgaaaa agacctgctg ggaaaacggg gcggcaggga
     2401 tcagtggaat atcggcctgc ccatttttct tttccttatc agtaaactcg ccgcttacac
     2461 tattcttgga gggttgctgg gcgggcttgg taagttattc cagttagatc cactttcgca
     2521 ggcggtactt caatttatca tcgccttctt cattatcggt aacgcgttgc gcatgttgaa
     2581 cgtccacccg ttattccgct atttcacctt tgaaccgccc gcgttcatca ccaggctcat
     2641 ccgcaagaaa acccgccagg cagccagttt caccacgccc atcatgctgg gagcgatgac
     2701 tgtgctgatc ccctgcgggg taacgcaaac gatgatggcg gcggcgattg ccacggggga
     2761 tgtgctgagc ggagcggcgc tgatgtttgc cttcacactt ggtgcaagcc cggtgttttt
     2821 cctgttgtct tatttcgcta ccaaactggg cgcacttacc gagaaatatt tcacccgtat
     2881 tgccgcggtc gtgttgattg ttttcggctt actggcagtg aacagtggtt taaacctgct
     2941 gggttcgcgc tactcgttaa gcaggctggc ggagaatgct gtcagtgcag tggagatacg
     3001 ccatcaaggg caggcgtcgg caacaccggg cactgacctt gtcttgaatg tggtcgatag
     3061 aggttaccgt ccaaagcgac tgcacgcgcc ggcgggtgtt ccgctaactt taacccttgt
     3121 gaccaaagag gtccattcct gttcacgcgc attcttgatc ccggcactgg attatggcgt
     3181 gattttactc gccaccggcg aggagaaagt gcagattccg ccgcaggaac caggcaccat
     3241 tttacccttc acctgttcca tggggatgta cagcggcgaa atcatttttg acctgtaacc
     3301 agagggataa aatgaaacga gttgagttgc gggttatgga tatgcattgc acaagctgcg
     3361 cgctgcgcct gcaaggactc gaggaccttc tcgcgggtgt tatccgcgtg gaggcttctt
     3421 accgtaaggg taaaatgacc gtgacctttg atgaaagcaa ggtcacagtg aaagatctgg
     3481 tcgaaggcgc gcgaaaactc ggttacaccg cggtggaata attaaatgac agctctctgt
     3541 ctggcaagtt attgctcagt gatagtgatg ctttcaatca catcaccgct gaatttggga
     3601 ttggtttgcg ggtcgcgcct ggtgatgtcc tttaccactt ccatgccgct gatgacctgc
     3661 ccaaaaatgg tgtaatcgcc gttcaggtgc tcaaccgggg cataggtgat aaaaaactgg
     3721 ctgccattgg tatctcgccc ggcgtttgcc atagccacca cacccgcctt atcaaacacc
     3781 aggtcgctgt cttcattttc aaactggtat cccgggccgc cgctgccggt gcccgagggg
     3841 tcgccggttt gcgccataaa gccggcgagc acgcggtgga aggtgacacc gtcaaagtag
     3901 ccttcacggg ctaaaaacac aaaactgttg accgttaccg gggctttgtc ggggtaaagc
     3961 tggatgacga actcaccgcc tttcgccatt ttcaccgtcg ccaggtattg tttggcgggg
     4021 tcgatggtca tcggcggcgc tgccgagtat tgtttgtagt tcatcacagc agcggtaggt
     4081 gaaactctgg cagcctggcg agatttgatc acatatccgc ctacaatggc cactgccagc
     4141 accagtaaaa cgatgaggat caacgtcagg tcggaatact cacggctgcg tttcttgctt
     4201 ttttgcctga cggctttact tctggaggtt ttatcgctca ttatttttcg ccttatgatt
     4261 caggatttgt gcagaatttc tttttccgcg cggttgattt caagaagaag atcttccacc
     4321 tcgcgcttgg cttcagctga agcgttaggc atgaactgga taaatttgag aagatcgcgc
     4381 gcctggcgca agatatccct gacattggcg gcgtaggaaa aatccagcca gccctgttca
     4441 gctggaagcg gcaggtcacg cgctttttgc atgagcgcgc gcgccttact gatacgttgc
     4501 gcgattggga tcaccctggc catatggatt ccgcagatgt tgcctggaca gttatagcat
     4561 agactgcaga attttgggag acaaatgaac agcccgttca gcgttggagc cgcagaagcg
     4621 aagggaacga gtagcgggaa atgccatcca gcgtgtaggg ggtgtggttg atgatagcca
     4681 tggcagcgtt tataccgctc tggatggatg ggccaatccc ttctcccatg tccagcgtgg
     4741 aaacccccaa ggcgtcgcca atcaacaagg cgtttccttt tctgagtagt ggctgcgcgg
     4801 ctctcaggta atacgaatga ccgagcggat taaagggatg atcgcggacc aacccatatg
     4861 catacagcct ctcggtgagc agttcccaat ggtgccggag ggtatctccg cgctccttta
     4921 acctcgcggc cgacccgcct acgcccacgt tcaccacgtt ttccgatttt gggaaatacc
     4981 aggcgtagcc aggtaaacct tgttgtaaaa accagaggtg gcacaggcgg ctggcgcccg
     5041 gataaatgaa ttcctcttcc tgggcgacga tcaggctgcc gcggactggt tgacccacag
     5101 gctcaaaaag agcgcgtttc accggacaat tcgttccgcc tgcgccgatg aggtaacgag
     5161 cgctgtattc ctcgtctaca atataatgct caccttcctg ccgtatctct ttcacctggt
     5221 gggtgaggac ttgtgcgcgc acgcggttga tcaaccattg gtcaaattcc acacggcgaa
     5281 tggcgtactg ccgcgtttgc aaagtgaaac gcaggccctt cagctcaatt aggaactggt
     5341 tgaatactga gaagttgccg gggtaggcgg cggggtcaag ctggatgtcg cgcagcacgc
     5401 gcggggtgac ccagcccgcg cagaccttgg gacgtggaaa ttctgcgcgg tcgagcagga
     5461 tgaattcaac cccgtgctgc ttgagttgcc aggcgcaggc agaaccagcc ggtccgccgc
     5521 cgacaatgag aacttctgtg cgcagcattg tctctcagct caaacgacag gctggctgcg
     5581 ggttgccctg gcgggtgaag accacctgcc ataactgctg gtgccgagaa cgaaaacccc
     5641 cggcgctggt gagcaggtaa taacgccaca tacggtagaa aacatcgcca tacctgggtt
     5701 ggagggacgg ccaggctttt tcaaaattgg cgctccaggc catcagggtc gggtcataat
     5761 acggcccaaa attatgccag tcttcgacca cgaacaactc ttccattgcc ctgccaagct
     5821 ggctgatgga aggcagcacg ccattgggaa atatgtagcg gtcggtccac ggttcaccac
     5881 gggtgatcgt gcggttgctg ccgatagtgt gaaagaagcc gataccttct ggtttcaggc
     5941 agcggtcaac caccagcata taatcgcggt aattcttgta acccacatgc tccatcacgc
     6001 cgatggaaat gaccgcgtcg tatgtaccgc tgacctcgcg gtaatcttgc aggcgcagct
     6061 ccaccggcag ccccttgcac aattccatgc ccagcgccac ctgttctttg gatacggtca
     6121 cgcccagcac ctcgacgccg tatttttcag cggcgaattt cgcaaaggag ccccagccgc
     6181 agccgagctc aagaatgcgc ataccgggat gagcgccaat tttgcggcat acaaggtcca
     6241 gtttggcttc ctgggcgtca tccaggtttt tagcgtcttt ccagtagccg caggtgtaca
     6301 ccaggcgctt atccagcatc gcctgataga gatcgttgcc caggtcgtaa tgcttgatgc
     6361 ctacttcaaa cgcccttcct ctggactggc ggttgaaaag gcgagatctg agcgcctgca
     6421 gcaaaaaacg agattcgttt ttaacctttt catccagcct ggcgagcaag gcgcgggtga
     6481 tgaactggtc aatggcaggg caatcccacc agccttccat ataggattct ccaagcccaa
     6541 gggtgccgtc gcgcagcacc cgatcgtaaa acgcggggtt gtgcacctgg atatcccagg
     6601 ggtcgggacc gttgactttg acctgcgcca ggtcaagaag ttcacgaacg acagattctg
     6661 cgcttgccat cataacctcc tttactgtta ttctagtaaa aacacctgaa ttattcaata
     6721 g
//
LOCUS       DAOTLW010000065         6425 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_11309_length_6425_cov_10.397959, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000065 DAOTLW010000000
VERSION     DAOTLW010000065.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6425)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6425
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11309_length_6425_cov_10.397959"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..847
                     /locus_tag="P5516_08120"
     CDS             <1..847
                     /locus_tag="P5516_08120"
                     /inference="COORDINATES: protein motif:HMM:NF014445.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HRT92436.1"
                     /translation="DWTNQPLVARVIADLEQTARARELVFLKIDPEVLRRTDSEKTTD
                     NPHEVQELLMARGWRCSPEQIQFKNTMLLHLDGEESDWLTRMKQKTRYNLRLAQKNGI
                     LVRKATMKDLPLLYRMFAETARRDNFIIRSEAYYLDVWSRFMQAGMAEGLIAEFAGQP
                     IAGMVLMFFGSRAWFVYGMSTSQHREKMPNYLLQWEAMRLAHEKGCTTYDLWGAPDTP
                     DPSDPMFGVYRFKEGLGAELVYTIGAWDFPLKPALYRLYHHVIPRVLSITRYVRRKKL
                     TQEVI"
     gene            844..1806
                     /locus_tag="P5516_08125"
     CDS             844..1806
                     /locus_tag="P5516_08125"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HRT92437.1"
                     /translation="MKIPRVPIAHLPTRIEYLPRLTDALGGPQLYCKRDDQTGLGFGG
                     NKTRKLEFLLAEARAQNADTLVSVGAIQSNHCRQVAALSARFGYQCILVLSGDEPALA
                     SGNLLLDQLFGAKIVWSTRDNRLQMLEDIVKTAQHEGRRPYKIPYGGSSAVGLMGYVE
                     AMKELELFPEKFDWIVIPSSSGGTQAGMLLGAKGAGFTGKILGICIEGNNRELHSTVA
                     SLMREGAELCGLPPVSEDTILVNDHYVGGGYGIMGTPEIEAIKLLALTEGLLLDPVYT
                     GRAAAGLIDLVRKGFFNKTDRVLFWHTGGTPALFAEPYLSLLQH"
     gene            1870..2700
                     /locus_tag="P5516_08130"
     CDS             1870..2700
                     /locus_tag="P5516_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92438.1"
                     /translation="MNIDVATTVKVILVLLGIAAIVSLTMAVKSIKAGYRLLFFQKRQ
                     LLVAHGWRLVLLALGLLLTGLVLIRFGEPMVYRYFPPSPTITQTPTVTITPTITNTPS
                     MTYTPTITLTLSQTYTPSLPEFIQATIQTPVGPDANAIFSPLSFSDTIKDGVVTTSYS
                     EFELPVGTLFGGFSYDRMAIGAQWTALWFYGDELICSETKAWNYAPGGYGYTDCSRPV
                     DAWRPGIYEVRIFVGQTWKQSGTFKILGEEPLVGNIPTSMGAQEPVESAVTATPPPTP
                     "
     gene            complement(2703..3635)
                     /locus_tag="P5516_08135"
     CDS             complement(2703..3635)
                     /locus_tag="P5516_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HRT92439.1"
                     /translation="MKLDLPDIYLVRPDVRQDLSNGQPVVALESAVITHGLPRPANLE
                     LALSMEAIVRGQGAIPATIAFIDGKIVIGASESELRRLAEENNPIKINPRNIGIALNS
                     GACGGTTVAATLMAARAAGIKVFATGGIGGVHRGNVFDISADLPALSSNPLVVVCAGA
                     KAILDIPATLENLETRGVPVLGFQTDEFPGFYSPGSGFKVDYRIESALEAFKIASAHW
                     RAGCRSAVLVCNPIPAEYGVDQQKIDDAIAQAVESANRKGITGAALSPYLLEELGKIT
                     AGKSLQANLVLLKNNALLAAQIARFFAKPGQISF"
     gene            3740..4636
                     /locus_tag="P5516_08140"
     CDS             3740..4636
                     /locus_tag="P5516_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92440.1"
                     /translation="MTKRKRILRDFKVYWVLPLIFTSMLGVSLVHYFGGNLNGISIIL
                     GGMVCLSLFAMRIFLNAYFDHPDSPISALSREDEQWEKLKEVKRNLLLQYSLLSLTAG
                     ATCTILLIARQALSTAGLIFLGLVLLGFLLCASPPLRLDRNGYGDLVEGVVTVNLVPA
                     FMVSLHQGEVPALLLPLTLPLLLLYIAAKIMFALKDYGFDIMHGQKSLVTLTGWKIAV
                     FLHNLLILGSFVLIGVFLLTRGLPWSLAWPAWMVLPFGIFQVWQVLRVAEGDKPAWKI
                     MLWLALGMVLMMIYFIQVPLWF"
     gene            4686..5138
                     /gene="bcp"
                     /locus_tag="P5516_08145"
     CDS             4686..5138
                     /gene="bcp"
                     /locus_tag="P5516_08145"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HRT92441.1"
                     /translation="MLKENTQAPDFSLPDETGVIHTLSQYAGRNIILYFYPKDDTPGC
                     TIEACNFRDDYSAYENSQVVILGVSPDSPKSHARFKAKYHLPFSLLSDEEHKVLEMYG
                     VWGKKKMFGKEYHGVLRTTYLIGKDGKIIKVFKNVKPAGHSAEILANL"
     gene            complement(5174..5491)
                     /gene="rsfS"
                     /locus_tag="P5516_08150"
     CDS             complement(5174..5491)
                     /gene="rsfS"
                     /locus_tag="P5516_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HRT92442.1"
                     /translation="MVNFLEEKKAEKIILLDIRELTTFTDYFIICNGTSDRMLQALAT
                     TLRDYIKKDLELIVNIEGESRDGWLVADLEDIVVHIFSPEMRNYYRLEQLWNKGKTIL
                     TLQ"
     gene            complement(5565..6155)
                     /gene="thpR"
                     /locus_tag="P5516_08155"
     CDS             complement(5565..6155)
                     /gene="thpR"
                     /locus_tag="P5516_08155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HRT92443.1"
                     /translation="MQTNIRAFIAIPLTREIHQQLGLFIQSYGLANRDAGFRPVKPEN
                     IHLTLKFLGDVKAASLPAISEHLDRLTVTLKQTSITISGLGAFPGWFKQPRVIWVGVQ
                     PVEPIQRMSEQIEKAISALGFPAERKPFSPHLTLARVNQQVAGQRYQEVITRLRNLQP
                     APVFGDMVADKIILFKSDLQPSGPVYSILSAHRLKG"
     gene            6246..6420
                     /gene="ssrS"
                     /locus_tag="P5516_08160"
     ncRNA           6246..6420
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="P5516_08160"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
BASE COUNT         1504 a         1563 c         1716 g         1642 t
ORIGIN      
        1 ggactggaca aatcaacctc tcgttgcgcg cgtaatcgct gacctggaac agaccgccag
       61 ggcaagagag ctggtttttc tcaagattga cccagaggta ctgagaagaa cggattctga
      121 gaagaccaca gataaccctc atgaggtgca agagctgctc atggctcgtg gctggaggtg
      181 ctccccggag caaattcagt tcaagaacac catgctgctc cacctggatg gagaagaatc
      241 agattggtta acgcggatga agcagaaaac ccgttacaac ctcaggttgg cgcagaaaaa
      301 tggcattttg gtacgcaagg caacaatgaa agacctgcct ttgttgtatc gcatgtttgc
      361 tgaaactgcg cggcgcgaca acttcatcat tcggtctgaa gcctattacc tggatgtgtg
      421 gtcgcgcttc atgcaggcgg gtatggcgga ggggttgatc gctgaattcg cggggcaacc
      481 catcgctgga atggtgttga tgttttttgg ctcgcgcgcc tggttcgtct atggcatgtc
      541 aaccagccag caccgtgaaa agatgcccaa ctatctgctg caatgggaag ccatgcgcct
      601 ggcgcacgaa aaaggctgca caacgtacga cctgtggggc gcgcctgaca caccagatcc
      661 atctgacccg atgtttggag tgtaccgttt taaagaaggg ctcggcgcgg aactggttta
      721 taccattgga gcctgggatt tccctcttaa acctgcgctt tatcgccttt atcaccatgt
      781 cattccgcgc gtgctttcga ttaccagata tgtccgccgg aaaaaattga ctcaggaggt
      841 catatgaaaa tccccagagt gccgatcgct catctaccca cccgtattga atacctgcca
      901 cgcctgaccg atgctttagg tggcccacaa ttgtattgta agcgcgatga ccagaccggg
      961 ttgggttttg gcggaaacaa gacgcgcaaa ctggagtttt tgttggctga agcgcgcgct
     1021 caaaacgcgg acaccctggt aagcgtggga gccatccaat ccaatcactg ccggcaggtg
     1081 gcggcgctgt ctgcccgatt cggttatcag tgcatcctgg tgttgtctgg agacgaacct
     1141 gctctcgcga gtgggaatct tttactggat cagctttttg gcgcgaaaat tgtgtggtcg
     1201 acgcgtgaca accgtttaca gatgctggag gatatcgtca agacagcgca acatgagggt
     1261 cgacgccctt ataaaatccc ttatggtggc tcaagcgcgg tgggcttgat ggggtatgtt
     1321 gaagccatga aagaactcga gctcttccca gagaagtttg actggattgt tattccctca
     1381 tccagcggag gaacccaggc gggcatgctg ctgggagcaa aaggcgcagg ctttaccggc
     1441 aagattttgg gaatctgtat tgaaggcaac aaccgggaat tacacagcac tgtcgccagc
     1501 ctgatgcgcg agggtgcgga attgtgcggt ttacctccag tgagtgaaga tacgatcctg
     1561 gtaaacgacc attatgttgg cggggggtac gggataatgg gcactccgga aattgaagca
     1621 attaagctcc tggctttgac tgaaggattg ctgttggacc cagtttacac cggaagggcc
     1681 gcggccggcc tgatagacct ggtcaggaag ggctttttta ataaaacgga tagggtcctt
     1741 ttctggcata ctggcggcac acccgcctta tttgcagaac catacctgtc gctgttacaa
     1801 cactaaaacc acctctctgc cccgagctgg ttttttgcgc atgctataat gcttttttgg
     1861 agagaagcat tgaatattga tgttgccaca acggtaaaag tgatcctggt tttgctgggg
     1921 attgcagcca tcgttagcct gacaatggca gttaaatcca tcaaagcagg ttacaggctg
     1981 ctttttttcc agaagcgtca attactggtc gctcacggct ggcgcctggt gctgctcgcg
     2041 cttgggttgc tgctcacagg gctggtatta atccgctttg gagaaccgat ggtctaccgc
     2101 tattttcctc cttctcctac catcacccaa acgcctaccg tgaccatcac acctacgatc
     2161 accaatacgc cttcgatgac ctataccccg accatcacct tgactttatc tcaaacttac
     2221 acaccttcat tgcctgaatt catccaggcg accattcaaa cccccgtcgg accggacgcg
     2281 aacgcgattt tcagcccatt gagtttttct gacaccataa aagacggtgt ggtgacgact
     2341 tcttacagtg agtttgaatt acccgtgggg acgttatttg gtggtttttc ttatgaccgt
     2401 atggctattg gagcgcagtg gacggcttta tggttctatg gagatgagtt gatctgttca
     2461 gaaaccaagg cgtggaatta tgctcccgga ggttatggtt atacggattg ttcacgccct
     2521 gtggacgcct ggcgtccggg gatctacgag gtgcgaattt ttgtcggaca gacctggaaa
     2581 cagtcaggca ccttcaagat cctcggagaa gaacctctgg ttggaaatat tccaacgagc
     2641 atgggagccc aagagcctgt tgaaagcgca gtaaccgcaa ccccgccgcc cactccttga
     2701 agttaaaaac tgatttgacc tggttttgca aagaatctgg cgatttgagc tgcgagcagc
     2761 gcattatttt ttaacagcac caagtttgcc tgcaggcttt tccccgctgt gattttgccc
     2821 agttcctcca acagatatgg agagagtgcc gcgcccgtga tccccttgcg gttggcggat
     2881 tcaacggctt gggcgatcgc gtcatcgatt ttctgctggt ctaccccata ttccgccgga
     2941 atggggttgc ataccagaac cgcgctgcgg cacccggcac gccaatgagc cgaagcgatt
     3001 ttaaaggctt ccagcgcaga ttcaatacgg tagtccactt taaaaccact gcctggtgaa
     3061 tagaaccccg ggaattcatc tgtttggaaa cccagaacag gtaccccgcg agtttccagg
     3121 ttttccagcg tggcaggaat atccaaaatg gctttggcac ccgcgcaaac gacgacgagc
     3181 gggttgctgg atagtgcagg taaatcggct gaaatatcaa aaacatttcc acgatgcact
     3241 ccgccgattc cgccggtagc gaagacctta atgccagcag cgcgggcagc catcaaagtt
     3301 gcggcgacgg tcgtaccgcc gcacgccccg ctgttcaaag cgatcccgat attgcgcggg
     3361 ttaattttga tggggttgtt ttcctctgcc aggcgccgga gttcagattc gctggcgcca
     3421 atgacgatct tgccgtcaat gaacgcgatg gtggcgggga tggctccctg accgcggaca
     3481 atcgcttcca ttgagagggc gagttccagg ttggcagggc ggggtagccc atgcgtgatg
     3541 actgctgact ccagggcaac gactggctgc ccatttgata agtcttggcg cacatcaggg
     3601 cgaacgagat aaatatctgg gaggtccagt ttcatggtca gtctccggct gtgatatagt
     3661 gcagtatata ttaaaatgat gaatagggca tccctattcg gaaaaaagca ggagaccaca
     3721 attgaccccg gggcagcaag tgacaaagcg taagcgtata ttgcgggatt ttaaggttta
     3781 ttgggttctt ccgctgattt ttaccagcat gctgggcgta agcctggtgc attactttgg
     3841 cggcaacctg aatgggatca gtattatcct gggtggcatg gtttgcctgt cgttgttcgc
     3901 gatgcgcatc ttcttgaacg cctattttga ccatccggac tctccgatct cggcgctgag
     3961 tcgcgaagat gaacaatggg aaaaattgaa agaggtcaaa cgcaacctct tgcttcagta
     4021 ctcattattg agtttaaccg ctggcgccac ctgcactatt cttcttatcg cgcgtcaggc
     4081 gctctccaca gcaggattga ttttcttagg gctggttttg ttaggctttc ttttatgcgc
     4141 tagcccacct ttacggctgg atagaaacgg ttacggagac ctggttgagg gggttgtcac
     4201 ggtgaacctg gttccggcct tcatggtcag cttgcaccag ggggaggttc cagccctgct
     4261 cttgccgttg actctgcctt tgttgctttt gtacattgcc gcgaaaatca tgttcgcctt
     4321 gaaagattat ggctttgaca tcatgcacgg gcagaagagt ctggttaccc tcacgggctg
     4381 gaaaatcgcg gttttccttc acaacttgct gattttggga tcttttgtgt tgattggcgt
     4441 ctttttgctg accagaggac tgccctggtc actggcctgg ccagcgtgga tggttctccc
     4501 atttgggatt tttcaggtat ggcaggtgct gcgcgtggcg gaaggtgata aaccagcctg
     4561 gaaaatcatg ctctggttgg cattagggat ggtcttgatg atgatctact ttatccaggt
     4621 gccgctatgg ttctgatgat ggttgaaggg gtttgatata atcagacagg aacaggagga
     4681 ataccatgct taaagaaaac acgcaggctc cagatttttc actgccagat gaaactggtg
     4741 ttatccacac actctcccaa tacgctggtc ggaatattat cctttatttc tacccgaagg
     4801 acgacacccc aggatgcacc attgaagcct gcaacttcag agatgattac agcgcttacg
     4861 aaaacagcca ggtggttatc ctgggggtga gccctgattc tcccaaatca cacgccagat
     4921 ttaaagccaa atatcactta ccgttttcac tgctttctga tgaagagcat aaagtgctgg
     4981 aaatgtatgg cgtctgggga aagaaaaaaa tgttcggcaa ggaataccac ggtgttttac
     5041 gcaccaccta ccttatcggc aaagacggga agattatcaa agtatttaaa aatgtaaaac
     5101 ccgctggcca cagcgccgaa atcctggcaa acctgtaaag acggcaaagc tgaggggagg
     5161 tcttacgctt tttttactgc agcgtgagga tcgttttgcc tttattccac agctgttcca
     5221 gccggtaata atttcgcatt tcgggcgaga agatatgcac cacgatatct tcaaggtcgg
     5281 ccaccagcca gccatctcgt gattctccct cgatgttgac aatcagctcc aggtctttct
     5341 taatgtaatc acgcagggtt gtagccagtg cttgcagcat acgatcgctg gttccgttgc
     5401 aaatgatgaa ataatctgta aaagtggtca gttcacggat atccagcaag ataatctttt
     5461 cagccttttt ttcttcgaga aaattgacga ttcgatgtgc tttttcgatt ggatccagga
     5521 ttcacctctc aatgatcgat tttctgaatt ctaacatagt tgttctaacc ttttaatcta
     5581 tgcgccgaaa ggatggaata aactggccct gagggttgaa ggtcagattt gaacagtatg
     5641 atcttatctg cgaccatatc accgaaaacg ggcgcgggtt ggaggttcct caggcgggta
     5701 atgacctctt gataacgttg accggcaact tgctggttaa cgcgggcaag cgtgagatga
     5761 ggggaaaagg gtttgcgctc cgcaggaaag ccaagcgcag atatcgcttt ttcgatttgt
     5821 tcgctcatgc gctgtatcgg ttcaaccggc tgcactccca cccaaataac gcggggttgt
     5881 ttgaaccaac caggaaaagc acccaagccg ctgatggtga tggaggtctg cttcaaggtt
     5941 actgtcaagc gatccaaatg ctcactgatg gcgggaaggc tggctgcttt tacatccccc
     6001 aggaatttta gggtcaggtg gatattttct ggctttaccg ggcgaaaacc ggcgtcgcgg
     6061 ttcgcgagcc catagctttg gatgaacaag ccaagttgtt gatgaatctc gcgggtgagc
     6121 ggaatggcga tgaaggcacg aatgttagtt tgcatctctg gttctcattc tacctggttt
     6181 ttctatgcgg aaagagacgt gatatggctt atctacccat tttacctaaa tcatggtata
     6241 agaacgatac cctgctgtgt tcgtgatgat gcgacaccgg ttttgagcca acacttctaa
     6301 aagggatcct cctctcaaca gggtaacgcg ataggcttcg gccaaggcgg atctctgtgg
     6361 caggatccac ctgcttcggc aggttcaaaa ctacgcagca cacggcatgg tggggtcttt
     6421 ttttc
//
LOCUS       DAOTLW010000066         6392 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_11372_length_6392_cov_4.012151, whole genome shotgun sequence.
ACCESSION   DAOTLW010000066 DAOTLW010000000
VERSION     DAOTLW010000066.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6392)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6392
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11372_length_6392_cov_4.012151"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(580..1902)
                     /locus_tag="P5516_08165"
     CDS             complement(580..1902)
                     /locus_tag="P5516_08165"
                     /inference="COORDINATES: protein motif:HMM:NF027890.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HRT92444.1"
                     /translation="MKQNLLKVVSLAVVLTFLLSACATAETGKFTASGSFSAVDTSIA
                     PELSGKVTEVSVHEGESVTTGQTLFKIDAEVSQAQYDQAAAAVSAAQAAVEASRGQAA
                     SAEAQYQLALQGALLQDMPNRQIAWTNTLPKDYREAWYFNETELIEAAQTQVESAEKA
                     LESARTELEDEQKKVSSKDFIAAEKRLALAQVTLAVANATLTQAQTAGNTDLKDAAIE
                     SKNAAQAEFDSALSDYQRMLTTSAADSIILARSRVAVAQVTLDNARDALLALQTGDQS
                     LQVVAAQKAAEAAASMVKQAEAGLDQAKQALNMAQLQLDRTTITSPVDGVVLTRSVEV
                     GDLAVAGGTVMRVANLDTLDLVVYLPEDQYGQVKIGDMVDITVDSFAGETFKGTIVRI
                     SDEAEFTPKNVQTESGRKSTVYAIKIQVQNLDHRLKPGMPADVEFTVD"
     gene            complement(1925..3058)
                     /locus_tag="P5516_08170"
     CDS             complement(1925..3058)
                     /locus_tag="P5516_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92445.1"
                     /translation="MNRLWTIMRKEIFHIWRDPRTLAMIVVLPALLLILLGFGISFEQ
                     QSVKMAVADFSKTDSSRNFIEQFTASKDFVYAYDVSNEDALIDLINRDLVDVGILIPE
                     NYDRDLLSGSQAKIPVYIDGSMQPTDSLTLQLKLNSITSMVAQDMLIEKVVKSGLSSS
                     LNLPVTGILKTLYNPNGDSKIYMIPGLIAILLQVQTLILSALAIVREREQGTMEQLIV
                     TPVRSWELMLGKIIPYLVVCIFNLFALLWLSQLFFGVTVAGSLGVLIGLSIIFILGSL
                     GMGVLISTISQSQMQAMYILLFVVLMPAIILSGLMFSRENMPAFTYWFSEMLPVTQYL
                     EITRGIMVRGVEASSLFFSSTLPLIILSVVYFVASILVFRKRI"
     gene            complement(3055..4017)
                     /locus_tag="P5516_08175"
     CDS             complement(3055..4017)
                     /locus_tag="P5516_08175"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92446.1"
                     /translation="MNSVEVNHLTRNFNDFFAVNDLSFYIPQGEIFGLLGPNGAGKTT
                     TIRVLCGIMLPSSGSVNVLGYDVTRNPNEVKSHIGYMSQKFSLYNDLTPVENITFYAS
                     IYGVPKKDRADRVKELIKMADLSGHERQLTRNLSGAWRQRLALACAIVHRPSMIFLDE
                     ATAGVDPVSRREFWDLIYDLASEGVSVLATTHYMDEAEYCNTIGMMYQGKLVALASPE
                     TLKNNLPGVLVQLECDRPGDVVKLVSGQDGVINTTIHGALVHLTLKDASVETLVKETL
                     AHAKIKIKAWEVIQPSLEDVFLTMVSEQLSTTETPVKQEKRRKA"
     gene            complement(4014..5081)
                     /locus_tag="P5516_08180"
     CDS             complement(4014..5081)
                     /locus_tag="P5516_08180"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92447.1"
                     /translation="MLRVTPVNVAQHLVNPSAGEYSESPTVNYQLDGSFRRTYMDFVI
                     TADHLSKQFVKRNTQLALDDLSMSVPRGILFGLVGPDGAGKTTTLRILSSVMLPSSGT
                     ANVMGFDTRKNPDPLRQQIGYMPQNFSLYPDLSVLENLNFFADIQQVTKERKPARITE
                     MLRFTRLEEFKHRKAGTLSGGMKKKLALACALVHEPQLLILDEPSTGVDPVSRRELWV
                     ILAEVVQHGVTVVVSTPYMDEAERCHQVSFLSHGRVLATSTPNELIKKLPFEILELKA
                     SPRKKMRQIVSETQGVLDWRPVGDSLRLNVPNNGASQKILQSLRERLDLDQLKVTLLR
                     PTRPSMEDVFVHLVNTQRSAA"
     gene            5148..6389
                     /locus_tag="P5516_08185"
     CDS             5148..6389
                     /locus_tag="P5516_08185"
                     /inference="COORDINATES: protein motif:HMM:NF019350.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HRT92448.1"
                     /translation="MKKTIEPGLLRIFRYFAGVAVVYFALLWGFTLFSAGWQKSVQFQ
                     FLGNFVVYFCLYGYLNLPIMEHRLKKLFLPLGLLVAAIFPVIGNFVYLIHPQQTDIFT
                     IINRSWLWLPVLFVPLVLIAWQYNFKAVLVYIVFTNGLELIVLISVMENFTYETLPLI
                     SVPLIRAFAFGTVGYIVNILIETQRMQNQKLIQTNLRLNQYMNTLEQLATNRERNRLA
                     AELHDTLAHTLSGLSINLEAIKTVVQPTQVEAQNMLDHSLNITRLGLNETRRVIKALR
                     AGPLDDLGLKMALKNLAQATSARCSIPVSLNCPDQLPVLPMDVEQSVYRITQEGLENV
                     VRHANASRAEVNVEVMADRLELTIHDDGVGFVPGQASNDEKFGLRGLQERAASIGGKL
                     LVDSQPGSGTLVRFVWERFND"
BASE COUNT         1514 a         1565 c         1691 g         1622 t
ORIGIN      
        1 aagaacggtg atgtatattt tgaacccatc tatcgttata aggaagactc gcctgcggaa
       61 gaaccttatt gggaaaaatt aggggaattg aaataaccaa ataattttta caaccatgct
      121 tatgttaagt aaaatgtaag cttgattgaa aaatccatca aatattgttg aatggtaaat
      181 accgctgcgt aatggattat gcaactttat accaattttg gcaaaaacat tttttggttc
      241 caaaatactt aaccctagtc tgaaaccttt cttttcaagt tatccaaaaa ctgaaattat
      301 gaaataacat aatgacaaat cgtgcgtggt caggagaccc gcttgaagga tcaacgctca
      361 ttctattagt cccggctgag cctactaata ttgaaaaaag gcaacccgtt cgggaaaact
      421 tacccgacgg gttgcgtaga ttttggcggg attccttttg gttatttgcg catgttttgg
      481 gctatcgctc tagtttcttc tccacttcgc atgaaaaatc catttatgga ttggaaaaat
      541 tcctagcgcg ctgactaata agttttgctt ggaaacgctc taatcgaccg taaactctac
      601 atccgcgggc atccccggtt taagtctgtg atcgagattt tgcacctgaa tcttgattgc
      661 atagaccgtg gattttctac ctgactcggt ctggacattt ttaggagtaa actcagcctc
      721 gtctgaaatg cgcacgattg tgcccttaaa ggtctcaccg gcaaaggagt ccaccgtgat
      781 atccaccata tcgccaatct tgacctgacc gtattgatct tctggcaggt aaaccaccag
      841 atcgagggtg tccaggttgg ccacgcgcat cacggtacca ccggcaacag caagatcgcc
      901 aacttccacg ctgcgggtca acacaacccc atccaccggg gaggtgattg tggtgcggtc
      961 aagctgcaat tgcgccatgt tgagtgcctg tttggcttgg tccaacccgg cttcggcttg
     1021 tttcaccatg ctggcggctg cttcggcggc tttctgggcg gccaccacct gcagggattg
     1081 gtcgccggtt tgcagggcga gcagggcatc gcgggcgtta tccaatgtta cctgggcgac
     1141 tgccacacgt gagcgcgcca ggatgatgga atccgcggcg gaggtggtca gcatgcgctg
     1201 gtagtctgat agggcagagt cgaactcggc ctgcgcagca tttttcgatt cgatggcggc
     1261 atctttaagg tcggtgttgc ctgcagtttg tgcctgggtc aaggttgcgt tggcgaccgc
     1321 cagggtcacc tgtgcgagcg ccaatctttt ctcagctgcg atgaaatcct tgctgctgac
     1381 tttcttctgc tcatcctcaa gttcggtgcg cgcggattcc aatgcctttt cggcgctctc
     1441 cacctgggtc tgggcggctt cgatcagttc ggtctcgttg aagtaccagg cttcccggta
     1501 atctttaggg agggtattgg tccaggcaat ttgacggttg ggcatatcct gtaagagcgc
     1561 gccttgcagg gcaagttggt actgagcctc tgcactggcg gcctgtccgc ggctggcttc
     1621 tacggcggct tgggcagcgc ttactgcggc ggcagcctgg tcatattgcg cctggctgac
     1681 ttccgcgtcg attttaaaga gggtttgtcc ggtggtgacg ctttcgcctt catgcacgct
     1741 gacttcggtg accttgccgc tgagttcggg cgcgatggaa gtgtcgacgg ctgaaaatga
     1801 gccggaggcg gtgaactttc ctgtttcggc agtggcgcag gcactcaata aaaaggtgag
     1861 cacgactgcc agactgacta ccttaagtaa attttgtttc atagggttct ccttgattag
     1921 taagttagat gcgttttctg aacaccagga tgctggcgac gaaatagacg acactcagga
     1981 tgatcaacgg taacgtggaa gagaagaaca gcgaactggc ttccacgccg cgcaccatga
     2041 tgccgcgcgt gatttcaagg tattgggtga ctggcagcat ttcgctgaac cagtaggtga
     2101 acgctggcat gttttctcgc gagaacatca accccgaaag gatgatggcc ggcatgagca
     2161 ccacgaacaa caaaatatac atggcctgca tctgggattg ggaaatggtt gagatcaaca
     2221 cacccattcc cagtgaaccg agaatgaaga tgatgcttaa tccgatcaac acgcccaagc
     2281 tgccggccac ggtgacgcca aagaagagtt gactcagcca caacagagca aacaggttga
     2341 agatgcacac caccaggtac gggatgatct tgccgagcat cagctcccac gaacgcaccg
     2401 gcgtgacgat caactgttcc atggtgccct gttcgcgctc acgcacgatg gccagcgccg
     2461 acaggatcaa ggtttgcact tgcagcagga tggcgatcaa cccggggatc atgtagatct
     2521 tggagtcacc gttcgggtta tacagcgttt taaggatccc agttacaggg aggttcaagc
     2581 tgctcgaaag acctgatttc accacttttt cgatcagcat atcctgggca accatggagg
     2641 tgatcgagtt gagcttaagc tgaagcgtca atgaatccgt tggctgcatc gaaccgtcga
     2701 tatagaccgg aattttggcc tgggagccac tcagaagatc ccggtcataa ttttcaggga
     2761 taagaatgcc aacatccacc aggtcgcggt tgatcagatc gatcaaagca tcttcattag
     2821 aaacatcata ggcatacaca aaatctttgc tggcagtgaa ttgttcgatg aaattacggc
     2881 tggagtcggt ttttgaaaaa tcggcgaccg ccatcttgac agactgctgt tcgaacgaaa
     2941 tgccgaatcc cagcaggatc agcagcagcg ccggaagtac cacgatcatc gccagcgtcc
     3001 gcggatcacg ccagatatga aaaatctcct tgcgcatgat tgtccacaat cggttcatgc
     3061 ttttctcctt ttttcttgct ttactggtgt ttcggtggtt gagagctgtt cagaaaccat
     3121 ggtcaggaat acatcctcga gcgaaggttg gatgacctcc catgccttga tctttatttt
     3181 ggcatgggcg agggtttctt tcaccagtgt ctcaacgctg gcgtctttca aggtaaggtg
     3241 caccaatgca ccatggatgg ttgtgttgat cacgccatcc tgaccggaaa cgagttttac
     3301 tacatctcca ggtcgatcgc actccaactg caccaagaca ccgggcaggt tgttctttaa
     3361 ggtttcaggg ctggccagtg ccaccagctt gccctggtac atcataccga tggtgttgca
     3421 atactcggct tcgtccatat agtgggtggt ggcgagcacg ctgaccccct cgctggcgag
     3481 atcgtagatc agatcccaga actcgcgtcg tgagacggga tccacccccg cggtagcctc
     3541 atccaggaag atcatggagg ggcggtggac gatggcgcaa gccaacgcga ggcgctgccg
     3601 ccatgcacca gaaaggttgc gggtgagttg acgttcatgt ccgctcaggt cagccatttt
     3661 gatcaattct tttacgcggt cagcccgatc tttcttgggc acaccgtaaa tggaagcata
     3721 aaaagtgatg ttttccacgg gagtcaggtc gttgtaaagt gaaaactttt gagacatgta
     3781 tccgatgtgg ctttttacct cgttcggatt gcgggtgacg tcataaccca acacattgac
     3841 tgaaccggag gagggcagca tgatgccgca caaaacccga atggtggtgg tttttcctgc
     3901 cccgttcggt ccgagcaagc caaagatctc gccctgcggg atgtagaaac tgaggtcgtt
     3961 gactgcgaag aaatcattga aattgcgggt caggtgattt acttcaactg aattcatgcc
     4021 gcactccgct gagtgttcac caggtggacg aacacatctt ccatactcgg acgcgtcggc
     4081 ctcagcaggg tcacttttaa ttggtcgaga tcaagccgtt cccgaaggga ttgtaagatc
     4141 ttttgcgatg cgccgttatt gggcacattc aaccgcaaag agtcgccgac cggacgccaa
     4201 tccaaaacac cctgggtctc gctgacgatc tgacgcattt tcttacgcgg actggctttc
     4261 agctccagaa tttcaaaggg tagtttcttg atcaactcat tgggcgtgct ggtagccagc
     4321 actcttccgt gggacaaaaa gcttacctgg tggcagcgtt ctgcctcatc catgtagggg
     4381 gtgctgacca caacagtaac gccgtgctgc accacttccg ccaggatgac ccacagttcg
     4441 cgcctcgaga ccgggtccac gccggtggag ggttcatcca ggatcaaaag ctgcggttcg
     4501 tgaacgagcg cgcaagcgag ggcaagcttc tttttcattc cccccgagag ggtgcctgcc
     4561 ttgcggtgct tgaactcttc caaacgggtg aagcgcagca tctcggtaat gcgggctggc
     4621 ttgcgctcct ttgttacctg ctggatatcg gcaaagaaat tcaggttctc gagcacgctc
     4681 aaatcgggat acaaactgaa gttttgcggc atgtatccga tctgctggcg caggggatcg
     4741 gggttcttac gagtgtcgaa tcccatcacg tttgcagtcc cgctcgaggg cagcatgact
     4801 gaagaaagga tgcgcaaggt ggtggttttt ccggcgccgt cagggccgac aagaccaaat
     4861 aaaatgccac gcggaacgct cattgagagg tcatcgagcg cgagctgtgt attgcgttta
     4921 acaaattgtt ttgataaatg atctgctgta atcacaaaat ccatataggt tctccgaaag
     4981 gacccatcaa gttgataatt gacggttgga ctttcactat actcaccagc agaaggattc
     5041 accagatgtt gagcgacatt caccggggtg acacgtaaca tgaccctgca tgactggcat
     5101 catctctctt gcaccccgag actgtgatta tgttatgata aaaaaagatg aaaaagacga
     5161 ttgaaccggg attactgaga atattccgat actttgccgg tgtggcggtg gtctattttg
     5221 ccctcttgtg gggttttacc ttgttttccg ccggttggca aaaatctgtg caattccaat
     5281 ttttagggaa tttcgtggtc tacttttgcc tgtatggcta tttaaacctg ccaattatgg
     5341 aacataggct gaaaaaactc tttttgcctt tggggctgtt ggtggccgcg atttttccag
     5401 tgattggtaa ttttgtttac ctgatccatc cacagcagac cgatattttc acgatcatta
     5461 accgcagctg gttgtggctg cccgtccttt ttgtccccct tgtgttgatc gcctggcagt
     5521 ataacttcaa agccgtgctg gtttacatcg tatttaccaa tggtttggaa ttgatcgtct
     5581 taatatcggt gatggaaaat tttacctatg aaacactgcc cttgataagc gtacccttga
     5641 ttcgcgcttt tgcatttggc acagtggggt atatcgtcaa tatcttgatc gaaacacaga
     5701 gaatgcaaaa ccaaaaattg atccagacca acctgaggtt gaatcagtac atgaacacgt
     5761 tggagcagct tgccaccaac cgagaacgca accggctggc tgccgaactg cacgacaccc
     5821 ttgcgcatac gctcagcggt ttgtcgatca accttgaagc gatcaaaacg gttgtgcagc
     5881 ccacacaggt tgaagctcaa aatatgttgg atcactcctt aaatatcacc cggcttgggt
     5941 tgaatgaaac ccgtcgagtg ataaaggcat tgcgtgccgg ccccctggat gatttgggtt
     6001 tgaaaatggc tttaaagaac ctcgcccagg ctacttcagc gcgttgttcg atccctgtct
     6061 cgttgaattg ccccgatcaa ttgcctgtgc tgccaatgga tgttgagcag agcgtttacc
     6121 gcatcacgca ggaagggctg gagaacgttg tgcggcatgc caatgccagt agggcagagg
     6181 taaatgttga ggtaatggca gatagattgg agctcaccat tcacgatgat ggcgtaggat
     6241 ttgttcccgg gcaggcatcc aacgatgaaa aatttggact gcgtggattg caggaacgcg
     6301 ctgcatccat tggcggtaaa ctgttggtag atagccagcc gggcagcgga actttggtcc
     6361 gctttgtatg ggagaggttc aatgattaaa gt
//
LOCUS       DAOTLW010000067         6148 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_11826_length_6148_cov_8.975710, whole genome shotgun sequence.
ACCESSION   DAOTLW010000067 DAOTLW010000000
VERSION     DAOTLW010000067.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6148)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6148
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_11826_length_6148_cov_8.975710"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            129..521
                     /gene="gcvH"
                     /locus_tag="P5516_08190"
     CDS             129..521
                     /gene="gcvH"
                     /locus_tag="P5516_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HRT92449.1"
                     /translation="MNTPKNLKYASSDEWVLIEGDVATLGVSDYAQDQLSDVVFFETT
                     VAVGDTLEKNAIIATIESVKAAADVKSSVSGKVIEINEALADTPELINSDPYGAAWMI
                     KVELANPQEAEVLMDAEAYAAFCETRGH"
     gene            521..1873
                     /gene="gcvPA"
                     /locus_tag="P5516_08195"
     CDS             521..1873
                     /gene="gcvPA"
                     /locus_tag="P5516_08195"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HRT92450.1"
                     /translation="MFIPHTEVEKKEMLKGIGVKDIKDLFTDVPEKFRYPKINLPPGV
                     TEMEVFAELNDYAAANEPVGEMVCFLGAGAYHHYVPAAVDALLRRGEFYTAYTPYQPE
                     ISQGTLQAIFEFQTLIANLTGMDVCNASHYDGATATAEAGVMAYHHFRGKRKKIILSP
                     AVNPQYQEVLCSYLCGFEDVDIHSGKLHGGLSDQPSDLGALIDDQTALVVVQYPDFLG
                     RIQDFSSLVYAAHQSGALVAFVVNPIALGLLKTPGELGADIVVGEGQPLGIPLSFGGP
                     YLGFFATRKEYVRKLSGRLVGETTDAQGRRGYVLTLTAREQHIRREKATSNICSNQGL
                     MALAATIYMSLVGKHGMRQVADLCYQKAHYAASEIAKIPGFQLVSKEPFFHEFVVKCP
                     SGIDEINRDLLDMGFIGGYDLGQTHPELTNQMLVAVTEMNSKEDIDAFAAALQEVTHG
                     "
     gene            1866..3329
                     /gene="gcvPB"
                     /locus_tag="P5516_08200"
     CDS             1866..3329
                     /gene="gcvPB"
                     /locus_tag="P5516_08200"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HRT92451.1"
                     /translation="MVEPTIYEISSNGRVGFRFPESDVPRVDLPDRLKRSDLPFPEVS
                     EVDVVRHFTHLSTLNYSIDKGFYPLGSCTMKYNPKVNEAAARIPGFSATHPLQPIETV
                     QGNLALMYGLQEFLKEISGFAAVTLQPSAGAQGEFVGVKIIKAYHQSRADEKRTRILV
                     PDSAHGTNPATSSMTGYEVVGVPSDARGNVDLASLRDICDETIAGLMLTNPNTLGLFD
                     ENVLEVIEIVHQVGGLVYGDGANLNALLGIARPGDLGFDVMHFNLHKTFSTPHGGGGP
                     GSGPVAVTQKLADFLPAPIIGIVEEATEDVPPLYGFITPPKTIGRVKAFHGQFGVLVR
                     AYTYIRMHGPDGLRKIAEHAVLNSNYLLSLLRHRYTLPYDRTYMHEFVLEGVWADAPD
                     VQALDIAKRLMDYNFHPPTNYFPLIVREALMIEPTETESKQELDAFATAMLKIADEAH
                     STPALLKEAPHNTPIGRLDEVKAAKELVLCCWLPEQE"
     gene            complement(3378..>6148)
                     /gene="alaS"
                     /locus_tag="P5516_08205"
     CDS             complement(3378..>6148)
                     /gene="alaS"
                     /locus_tag="P5516_08205"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HRT92452.1"
                     /translation="HAHILEERTGEMDMKQMTGAEIRQGFIDFFKQKGHTYVPSSSLV
                     PGGDQTLLFTNAGMVQFKDVFLGIDKRDYTRAVNSQKCMRVAGKHNDLDDVGRDDSHH
                     TFFEMLGNWSFGDYYKKEAITWAWELLTGVWGLHPEHLYATVFKDDKGNIPADVEAAE
                     IWRQQPGMSPEHVLSFGRKENFWEMADTGPCGPCSEIHIDRGESFCNKKEVPGHICKV
                     NGDCPRFLEIWNLVFIQYNRLDQDTFEPLPARHVDTGMGFERIVAILQNVNSNYRTDL
                     FTPLLDKIRELTGMSVAAMNEDFTPYRVIADHTRAASFLIADGVVPGNIGRNYICRMI
                     IRRAARFASKLGLTEPFMAQIASIVIDQYRAAYPELEKNRKTILDNLTREELRFQRTV
                     EGGVSQLEELMDELKKSGKKVLDGSKAFELYATHGLPLEITRDIAREVGLDVDEPGFK
                     KAMEEHRLHSGAGKAIGTMGGNEVELYRKVYVELVESGKIPAGGVSYDPYHALEIETQ
                     IVGLFSEGEVLSEAPRGSEVEVILPKTGFYIEAGGQVGDTGTLTALDGSWEIKVEAVR
                     RPAAGIIIHIGEVVHGTVRNGDKVLMKVDRQRRMDIMRNHTATHLLHAQLHKVLGNHA
                     RQAGSLVAPDRLRFDFTHPEALTQEQLDEIESGVNREILASHALVVKEKTLQDAMAEG
                     AMALFGEKYGEKVRTVTIDEENPISYELCGGTHVENTGDIGTFLILSEGSAAAGIRRI
                     EGVTGRGAYEMIQARNKILRRAARLTNSSMEEVVAKINSLQDEIESLQVELGRVRQEW
                     VKADFEGKLDNILYIAGIPVLSVLLKGADIETMRQMADIFRHKHPSGIVAIGSIINEK
                     PMIICSVTEDLVKRGLNAGELARQAAAIVGGSGGGRPVMAQAGGKDPARLPEALSSVI
                     ETLKRKLT"
BASE COUNT         1478 a         1654 c         1442 g         1574 t
ORIGIN      
        1 attcttgact tttttccaac tgctttatag aatgagattg actgcggcct aaaattcgca
       61 gtgaaatggg gttatttcct catcaatcga cctgcgtaat accaactact ttcatatcag
      121 gaggtaagat gaacacaccg aaaaacctta aatacgcgag ttcagatgaa tgggtactca
      181 tcgaagggga tgtcgctaca ctgggagttt ctgattacgc tcaggatcag ctctcagatg
      241 tggtgttttt tgaaaccaca gttgccgtgg gtgataccct ggagaaaaat gccatcatcg
      301 caaccatcga atcggtgaaa gctgctgcgg atgtcaaatc gtccgtaagc ggaaaagtta
      361 tcgaaatcaa tgaagccctg gctgatacac ccgaactgat caattcagat ccttatggcg
      421 ccgcctggat gatcaaagtg gaacttgcaa atccacagga ggctgaggtt cttatggacg
      481 cagaagccta tgcggctttc tgcgagacaa gaggtcatta atgttcatcc ctcataccga
      541 ggttgaaaag aaagagatgc tgaaaggcat cggtgtgaag gacatcaaag acctgttcac
      601 tgatgtccca gaaaaattcc gttacccaaa aatcaacctt cctcctggtg taaccgaaat
      661 ggaggtcttt gcggaattaa atgactacgc cgcagccaat gaaccagtag gtgagatggt
      721 gtgttttctc ggagcaggcg catatcacca ctatgtacca gcggcggttg atgcgttact
      781 ccgacgcggc gagttttata ctgcgtatac accctaccag cctgagatct cacagggaac
      841 cttgcaggcg atcttcgagt ttcaaacgct tatcgccaac ctcacgggta tggatgtgtg
      901 taatgcctcg cattatgatg gcgcaaccgc taccgccgaa gctggagtga tggcttatca
      961 ccattttcga ggtaagcgca aaaaaatcat cctgtcacca gcggtcaacc cgcaatatca
     1021 ggaagtactc tgttcgtatc tttgcggatt cgaagacgtc gatatccatt ctggcaaact
     1081 gcatggtggc ctttccgacc aaccttccga tctgggtgcg ctcatcgatg accaaacagc
     1141 tttagtggtc gttcaatacc ccgacttttt agggcggatt caggactttt cctccttggt
     1201 gtatgccgct caccaatctg gcgcgctggt ggcatttgtg gtcaacccaa ttgcgctggg
     1261 tttgctcaaa acacccggcg agctgggagc tgatattgtc gtcggagaag gtcaaccttt
     1321 aggcatacct ctatcttttg gcggtccata cctgggtttc ttcgccacgc gcaaagagta
     1381 tgttcgaaaa ctttcaggtc gcctggtcgg tgagacgact gatgcccagg gaaggcgtgg
     1441 ttatgttctc accttaactg caagagagca gcatatcagg cgagagaaag caacctctaa
     1501 tatctgttcc aaccagggct taatggccct cgcggcaact atttacatga gcctggtcgg
     1561 taaacatgga atgcgacagg tagccgacct gtgctaccaa aaagcccact acgccgcgtc
     1621 tgaaatcgca aaaatccccg ggtttcaact tgtgtcaaag gaaccatttt tccatgagtt
     1681 tgtggttaaa tgcccgtctg gcatcgatga gatcaaccgt gatttattgg acatgggttt
     1741 tatcggtggc tatgacctcg gccaaacgca tcctgaattg accaaccaaa tgcttgtggc
     1801 ggtaaccgaa atgaactcaa aagaggatat cgatgccttt gcagccgcat tacaggaggt
     1861 cacccatggt tgagcccacg atctacgaaa tttcttccaa cggcagggtg ggttttcgct
     1921 ttccagaatc ggacgtacct cgtgttgacc tgcctgacag gctgaaacgg tctgacctgc
     1981 cctttcctga agtatccgaa gtggatgtgg tgcgtcattt cactcacctc tcgaccttga
     2041 attacagtat tgataaaggg ttctatcctc tcgggtcttg caccatgaaa tacaacccaa
     2101 aggtgaatga ggccgcggca cgcatccctg gttttagcgc gacgcacccg ttgcaaccga
     2161 tcgagacagt tcaaggcaac ctggcgttga tgtacggcct gcaggaattc ttaaaagaaa
     2221 ttagcggttt cgctgccgtc actctgcagc catcagctgg tgcgcaaggc gaatttgtgg
     2281 gggttaagat tatcaaagcc tatcaccaat cccgggctga tgaaaaacgc acccgcatcc
     2341 ttgttcctga ttccgcccac ggcacaaacc cagccacctc ctcgatgacg ggttacgaag
     2401 tggtcggtgt gccctccgac gctcgcggca atgtcgatct cgccagcctg agagacatct
     2461 gtgatgaaac catcgcgggg ctgatgttga ccaacccaaa cacattgggg ttatttgacg
     2521 aaaatgtgct tgaggtgatc gagatcgtgc accaggtggg cggcctggtt tatggagacg
     2581 gcgctaacct gaacgctctt ttagggattg cccgcccggg agacctgggc tttgacgtga
     2641 tgcatttcaa cctgcataaa accttttcca ctccgcatgg gggcggcggt ccgggctctg
     2701 gcccggtggc cgttacgcaa aaactggctg attttctccc cgcgcctatc atcggtattg
     2761 tcgaagaagc cacggaagat gtccctcctc tatacggctt catcactccc cccaaaacaa
     2821 tcgggcgtgt aaaagccttt catggtcagt ttggtgtgct ggtacgggcg tacacctata
     2881 tacgcatgca cggaccagat gggttgcgga agatcgctga acacgcggtt ttaaactcaa
     2941 actacctgct ttcactcctg cgccacaggt acacactgcc ctatgaccgg acatatatgc
     3001 atgaatttgt gctcgaaggt gtttgggcag acgctcctga cgttcaagcg ttggatatcg
     3061 ccaagcgtct gatggactat aacttccacc ctcccacaaa ttatttccct ctcatcgtcc
     3121 gtgaagcctt gatgatcgaa ccaaccgaaa ctgaaagcaa acaagaactg gatgccttcg
     3181 ccactgccat gctaaaaatc gcggatgagg cccattcaac accggctctc ctgaaggagg
     3241 ctccgcacaa tacaccaatt ggccgccttg acgaggtaaa agccgccaaa gaactcgttc
     3301 tctgctgctg gttaccagaa caagaataag tgagaataag ccaaaaaaag aactcccttg
     3361 cagggagttc ttttggttta agtcagtttt cgtttgagcg tttcaatcac actggaaagc
     3421 gcttccggca gcctggcagg atcttttccg cctgcctgcg ccattaccgg acgccctccg
     3481 ccactgccgc ccacaatagc cgctgcctgc ctggcgagtt cgccggcatt gagcccgcgc
     3541 tttaccaggt cttcagtgac cgagcatatg atcattggtt tttcgttaat gatcgacccg
     3601 atggccacga ttccagaagg gtgcttatga cgaaagatgt cagccatttg ccgcatcgtc
     3661 tcaatgtccg ctcccttcaa caatacagag agcactggta tgccagcaat gtagagaatg
     3721 ttgtccagtt tgccttcaaa atccgctttg acccattctt ggcgtacgcg ccccaactca
     3781 acctgtaaag actcgatttc atcctgtaga gaattgattt tcgcaaccac ttcttccata
     3841 gacgaattgg tcaaacgcgc cgcccggcgc agtatcttat tgcgcgcctg tatcatctca
     3901 tatgcgcccc gcccggttac tccttcaatg cgtcgaatcc cggctgccgc gctgccctca
     3961 ctgagaatca agaaggtacc tatatctcca gtattttcaa cgtgcgtacc gccgcacaac
     4021 tcgtatgaaa tggggttttc ttcatcgatg gttacagtgc ggactttttc gccatacttc
     4081 tcgccaaata aggccatcgc tccttcagcc atcgcgtcct gtaatgtttt ctctttgacc
     4141 actaatgcat gtgaagcgag tatttcacgg ttaactccgc tttcaatttc atccaactgc
     4201 tcctgggtta aggcttcagg gtgagtaaaa tcaaaccgca ggcgatctgg agcgaccaat
     4261 gagcccgcct gccgagcgtg gttgcctaac accttgtgca attgcgcatg cagtaagtga
     4321 gtggcggtat ggttgcgcat aatatccatt cgccgttgtc ggtcgacttt catgagaacc
     4381 ttgtcaccgt ttctgacagt tccgtgaacc acttcaccga tatggatgat gatgcccgca
     4441 gctggtctgc gtactgcctc cactttgatt tcccaactgc catccagtgc cgtaagggtg
     4501 cctgtatcgc ccacttgccc gccggcttca atataaaacc ctgtcttggg caagatgact
     4561 tctacttcac taccgcgggg tgcttcagat aatacttctc cttcagaaaa caggcccaca
     4621 atctgtgttt caatttcaag cgcgtgataa gggtcgtacg acacgccgcc tgcggggatt
     4681 tttcctgatt caaccaattc cacataaact tttcgataga gttcaacctc attgccaccc
     4741 attgtgccta ttgccttacc cgcgcctgag tgaagtctgt gctcttccat ggctttttta
     4801 aacccgggtt catccacgtc caggccaact tcgcgcgcga tatccctggt aatttccagc
     4861 ggcagcccat gagtggcgta aagttcaaat gccttgctgc catccaatac cttcttcccg
     4921 ctttttttga gttcgtccat cagctcttct aattggctga cgccgccttc tactgtgcgc
     4981 tggaaacgca gttcttctcg tgtaaggttg tccagtatcg ttttccgatt tttctccagc
     5041 tcgggatacg ccgcccggta ttgatcaata acaatactgg caatttgcgc cataaacggc
     5101 tcggttaaac ccagcttgga cgcgaaacgc gccgcgcggc gaataatcat gcggcaaatg
     5161 taatttctgc cgatatttcc cggaacgact ccatcggcga tcaaaaaaga ggccgcgcgc
     5221 gtatggtccg caatcacccg gtatggcgtg aaatcttcgt tcatcgctgc cacgctcatc
     5281 cctgtaagct cgcgtatctt atccaataag ggtgtgaaaa ggtctgttcg gtaattggag
     5341 tttacattct gtagaatcgc tacaatacgt tcaaacccca taccggtatc cacgtgcctg
     5401 gcaggcaggg gttcaaatgt gtcttgatca aggcgattgt attgaataaa gaccaggttc
     5461 catatttcca ggaagcgagg gcaatctcca ttaaccttgc aaatgtgccc ggggacctct
     5521 ttcttattac aaaaggactc tcctctatcg atgtgaatct cgctgcaagg cccgcaagga
     5581 cctgtatccg ccatctccca gaaattttct ttacgtccaa aagaaagtac atgctcaggg
     5641 ctcattccgg gttgctgccg ccagatttcc gcggcctcta cgtcagccgg aatattcccc
     5701 ttgtcgtctt taaatactgt ggcatataaa tgctcaggat gaagtcccca aacgcctgtc
     5761 aacagctccc aagcccaggt tattgcctct tttttgtaat aatcaccaaa cgaccagtta
     5821 ccaagcatct caaaaaaggt gtgatgcgaa tcatcacgcc cgacatcgtc caggtcatta
     5881 tgttttccgg ctacacgcat acatttttga gaattgaccg cccttgtgta atctcgcttg
     5941 tcaatgccga ggaacacgtc tttaaactgc accattccag cgttggtaaa aagaagggtt
     6001 tggtctcctc caggcaccaa agaggaagaa ggcacatagg tatgcccttt ttgcttaaag
     6061 aaatcaataa aaccctgcct gatttccgct ccagtcattt gtttcatatc catttcacct
     6121 gttctttcct caagtatatg cgcatgaa
//
LOCUS       DAOTLW010000068         6054 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_12017_length_6054_cov_9.759960, whole genome shotgun sequence.
ACCESSION   DAOTLW010000068 DAOTLW010000000
VERSION     DAOTLW010000068.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6054
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12017_length_6054_cov_9.759960"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..239
                     /locus_tag="P5516_08210"
     CDS             <1..239
                     /locus_tag="P5516_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT92453.1"
                     /translation="IGDEGVDLAVLPIGDNYTMGPDDALLAVRLLRAKMVVPIHYDTF
                     EVIRQDAAAWAERVRVNTPTQVILLKPGDQVSVE"
     gene            348..1355
                     /locus_tag="P5516_08215"
     CDS             348..1355
                     /locus_tag="P5516_08215"
                     /inference="COORDINATES: protein motif:HMM:NF016362.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease"
                     /protein_id="HRT92454.1"
                     /translation="MMETILELLTTAGRFLVGMWPVMFVVLLFGIFKRRDGFGTMIRL
                     SIKSLVIAWVFFAILRVVFNYFHMDTLQLIEEPENTKYFLIVGLFLLPLALAIVFEEA
                     RKRRTIKSIEDMQLLSPSEFEKLVAKTYREQGHSVEIVGATGDHGIDLVVHTNKGETW
                     LVQCKKYRGKVGEPVVRDFYGVLRASNADAGAIVTTGLITPQARLWAEGKPIFLYDGR
                     AFLKIIESTRLRRVLPQEVRKPEPATPAKQSWFGSALKTASATASAAAPVTLTGMVEH
                     SVEMEAQDKTPFSKEANIPDCPVCGTSMILKTTPRFLLKPKKAYICVNAPECEETFPV
                     E"
     gene            complement(1381..2001)
                     /locus_tag="P5516_08220"
     CDS             complement(1381..2001)
                     /locus_tag="P5516_08220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92455.1"
                     /translation="MTVHLVRLGGSILRHEFRIEGMVQPSLEALVTRTIDRFYAVTGP
                     LAASLGVEKVYTVHLEQKGGNNYAPGNRLNLNTLNLTEWTITHELAHALDAAHGWRLS
                     QQMRAHTGSGFLFKALHYARPDWKAFWYRVGSPPPPCGVDKNFNSLEDFAETVTAYIF
                     PAEAHRRAEQRGYAYEKWGYTHFHETPRGQFFANLLNREAIKTPSG"
     gene            2270..3547
                     /locus_tag="P5516_08225"
     CDS             2270..3547
                     /locus_tag="P5516_08225"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004420 - hydroxymethylglutaryl-CoA
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0016054 - organic acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HRT92456.1"
                     /translation="MESKTPRFYELSISSRLEQLAREADLPLEDLLPLGGKPILTTET
                     ADHMIENAVGVYGMPLGIARNFLINGREVLVPMAIEEPSVVAGASFMAKLARAGGGFV
                     ATTTAPEMIGQLQLLDIADPAAAKASVLAHKQELLELAGKEHPTLSRFGGGPRDIEMR
                     EIHTSPIGPFLVMHLVYDVRDAMGANAINTALEALAPRVAQISGGRVHLRILSNLADK
                     RLARAECTIPLAVLGFGEYTPEAVRDGIIEAWAFAAADPYRAATHNKGIMNGVDAVIL
                     ATGNDWRAVEAGAHAYAARDGRYTSLTTWEVTADGSLKGSLEMPMAVGIVGGATRSNP
                     AAAAAIKLLKVASAAELAQVIASVGLAQNLAALRALATEGIQRGHMGLQARQVALAAG
                     AAGDEVDRLAQQLVKENNIRSDRAVQILLEWRK"
     gene            3552..4628
                     /locus_tag="P5516_08230"
     CDS             3552..4628
                     /locus_tag="P5516_08230"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HRT92457.1"
                     /translation="MEKNQPASKLLRTDRKTGIVGYGAYIPRYRLPGSEVAMLWTGSP
                     KGSPVKEKAVAGLDEDVITMSIEAARNALVRANIDASELRAVWVGSESHPYAVKPSGT
                     LVAEAIGASNDIQAGDWEFACKAGTEAMVAAIALVGSGMGRYALAIGMDTAQGKPGDA
                     LEYTASSGGAAFVIGPREEAVVNIEATYSFVSDTPDFWRRPEQKYPEHGQRFTGDPAY
                     FKHVESAGRVMLEELGMKASDFTYAVFHQPNPRFPLKAGKDLGFTEEQIKPGLLVPVI
                     GNTYAGASMVGLTAILDIAKPGDRILQVSYGSGAGSDAFYLEVTEKIGERRELAPSTA
                     DYLARRTEINYATYARFRGKIDMG"
     gene            4638..5801
                     /locus_tag="P5516_08235"
     CDS             4638..5801
                     /locus_tag="P5516_08235"
                     /inference="COORDINATES: protein motif:HMM:NF012336.2"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HRT92458.1"
                     /translation="MTEVVIAGIGQIPVGEHYTLSLRSMGVQALTRALKDSGGLKPDA
                     LYIGNLLSSMISHQSNLGALFTDYGRLDSIEAFTTEAAGASGAAALRLGVLAILSGQA
                     DTVAVLGVEKWSDLVRADAEAMITQELDYDYESIPGLTSTSQAALIQQRYLHEHDLEQ
                     NALSGFALLAHANAVHNPFAFYRNPLTEEGYARAGMLSDPITIFDGATYADGAAALVL
                     TRRELLPRGYAHPVISVMGSGASSDALSWHDRTEPMSFTAAVRAIDRAVQQAGLSKEQ
                     IDLFELDDAFSIYAALTLEGVGLADKGAAWKMARDGEYNIGGRLPILTMGGCLARGNP
                     IGAKGVYQVVEAVTQLRGAAGANQVKGARAALSLALGGPASTAIAHVLKRESE"
BASE COUNT         1363 a         1586 c         1845 g         1260 t
ORIGIN      
        1 tgattgggga tgaaggtgtc gatctggcgg tgctgccgat tggcgataat tacaccatgg
       61 gaccggatga cgcgctgctg gccgtgcggc tgttgcgcgc aaaaatggtg gtgccgattc
      121 actacgacac ctttgaggtc atccgccagg acgcggccgc ctgggcagaa agagttcggg
      181 tgaacacgcc aacacaggtg attttactga agccgggcga tcaggtttct gtggaataga
      241 gatttcacgc cagaagaggg aatgccagaa tccgtctgtg aaagacggat tctggcatta
      301 ttcgtgcaac aagaaaagaa atacgttttg ggctttgtgg aggtttgatg atggaaacaa
      361 tacttgaact tttaaccacc gccgggaggt ttctggttgg catgtggccg gtgatgttcg
      421 tggtgctgct ttttgggatt ttcaagcgcc gagacggttt tggcaccatg atcaggcttt
      481 ccattaaatc actggtgatc gcctgggttt tctttgcgat attaagggtg gtgttcaatt
      541 atttccatat ggataccctc cagttaattg aggaaccaga gaacaccaag tatttcctga
      601 ttgtggggct ctttttgctg ccattggcgc ttgccattgt gtttgaggaa gcccgtaaac
      661 gccggacgat caaatcgatc gaagacatgc agctcctctc gccttcagaa tttgaaaaac
      721 tggtagccaa gacttaccgc gagcagggac attcggtcga gattgtggga gcgactggcg
      781 accacggcat cgacctggtg gtgcacacca acaagggaga aacctggctg gtgcagtgca
      841 agaagtatcg cggaaaagtg ggtgagccgg ttgttcgaga cttttatggc gtgttgcggg
      901 cgtctaatgc tgacgcgggt gccattgtca ccacgggtct gatcacccca caggcgcgac
      961 tgtgggcaga gggcaagccc atcttcctgt atgatgggcg ggctttcttg aagatcatcg
     1021 aaagcaccag gctgcgccgt gtgttaccgc aggaagtgcg caaacctgaa cccgccactc
     1081 ccgccaagca gagctggttt gggtctgcct taaaaacagc atcagcaact gccagcgcgg
     1141 cagcaccggt gaccttgaca ggtatggttg aacattccgt ggagatggaa gcgcaggata
     1201 aaacgccctt ctcaaaagaa gccaacatcc ctgattgccc ggtttgcggt acaagcatga
     1261 ttctaaaaac cacgccacgc tttttgctca agccaaagaa ggcctatatt tgtgttaacg
     1321 cgccggaatg tgaagaaaca ttccctgtgg aataagaaaa cacttcaaaa catcacgccc
     1381 tcaacccgag ggcgttttta ttgcttcgcg gttcagcagg ttggcaaaaa actgacctcg
     1441 cggggtttcg tggaaatggg tgtaacccca tttctcatag gcgtatcccc gttgttcagc
     1501 ccgccggtgg gcttcagcgg ggaagatgta ggcggtgacc gtttcggcga aatcttccag
     1561 cgaattgaaa ttcttgtcca ctccgcaagg gggaggaggg ctgcctaccc ggtaccaaaa
     1621 agctttccag tcaggccggg cgtaatggag agctttgaat aaaaaaccac tgccggtgtg
     1681 tgcgcgcatc tgctgtgaaa gccgccagcc atgcgccgcg tcgagggcgt gcgccagttc
     1741 atgggtaatg gtccactcgg tcaggttcag cgtgttaagg ttcaacctgt taccaggagc
     1801 gtaattgttc ccgcccttct gctccaggtg cacggtgtag accttctcga cccccaagct
     1861 ggcagcgaga ggaccggtga ccgcgtagaa gcgatcaatg gtacgggtga ccagggcttc
     1921 cagcgagggc tgaaccatac cttcaatacg aaattcgtgt cgcaggatgg aaccgcccag
     1981 gcgtaccaga tgaactgtca tttccggctc ccttgtctta ctctacataa gaggtgactg
     2041 tgtagcgaaa aggagaatgg agcgggaaga tcccgggaaa ggcgcaagtg cggaacgggc
     2101 gagggaaaaa cacgctgatt catttgatga ttaatcctac tataaaaacg ctgtactccc
     2161 aggaaattcg tttttcaaca atggtagcac ggaaaggcaa taatcctgat aggttttgcc
     2221 agtatcgcgc gtgtagtatt gagacaatac actggctgac cggagctgca tggagagcaa
     2281 aacacccagg ttttatgaat tatccatctc ctcccgcctg gaacaactcg cgcgggaggc
     2341 ggatttaccg ttagaggact tgttgccgct tggcggtaag cctatactca ccacagaaac
     2401 cgcggaccat atgatcgaga acgcggtggg agtatacggc atgccgctcg gcatcgcgcg
     2461 caacttcctc atcaacgggc gcgaggtttt ggtgccgatg gcaatcgaag agccatcagt
     2521 cgtggcgggg gcttctttta tggcgaaact ggcccgcgcg ggcggcggct ttgtcgctac
     2581 gacaaccgcg cctgagatga taggtcagct gcagttgctg gatatcgccg accccgctgc
     2641 ggcaaaggcc agtgttctgg cgcataagca ggaattgctt gagctggcgg gcaaagaaca
     2701 cccgacgctg tcgcgctttg gcggcggtcc gagggatatt gaaatgcgcg agatccacac
     2761 ttcgcctatc ggtccctttc tggtgatgca cctggtttac gatgtgcgtg atgccatggg
     2821 ggcgaacgcc atcaacaccg ccctggaggc gcttgcgccg cgcgtggcgc agatcagcgg
     2881 tggacgagtg cacctgcgca tcctctctaa cctggcggat aaacgcctgg cccgcgcgga
     2941 gtgcaccatc ccgctggcgg tgttgggttt tggagaatac acacccgaag cggtacgcga
     3001 tggcatcatt gaagcctggg ctttcgctgc ggcggatcct tatcgcgcag caacgcacaa
     3061 caaaggcatt atgaatggcg ttgacgcggt tattctggct accggtaacg actggcgggc
     3121 ggtggaagcc ggcgcgcacg cttacgctgc ccgtgatggg cggtacacca gtctgaccac
     3181 ctgggaggta accgcagatg gcagcctgaa aggcagcctg gaaatgccca tggcagtggg
     3241 gatcgtaggc ggcgccaccc gctctaaccc ggccgcggcc gcggcgatca aactgctaaa
     3301 ggtagcatcc gcagccgaac tggcacaggt gatcgcttct gttggcctgg cgcaaaacct
     3361 ggccgctcta cgcgcgctgg caaccgaggg gatccagcgc gggcacatgg gactgcaagc
     3421 gcgccaggta gcgctggcag caggcgcagc tggtgatgag gtcgaccgcc tggcgcagca
     3481 actggtaaag gaaaacaata tccgctcaga tagggcggta caaattttgc tggagtggag
     3541 aaagtaaagc tatggagaaa aatcaacccg catcaaagct attacggact gaccggaaaa
     3601 ccggtattgt tggctatggc gcctatattc cccgttaccg cttaccgggg agcgaagtgg
     3661 ccatgctatg gactggatcg cccaaagggt cgccagtaaa ggagaaagcg gtcgctggac
     3721 tggatgaaga cgtcatcacc atgtcaattg aggcggcgcg caacgcgctc gtgcgcgcga
     3781 atatcgacgc ttctgagctg cgggccgtgt gggtggggtc tgaatctcat ccttatgctg
     3841 tgaagcccag cggcacgctg gtggcagaag cgattggcgc ttccaatgac atccaggcgg
     3901 gagattggga gtttgcctgc aaggctggta cagaagccat ggtggcagcc atcgcgctgg
     3961 tgggctctgg tatgggcagg tacgcgctgg ccatcggcat ggatactgcc cagggcaagc
     4021 ctggagacgc gctggaatat accgccagca gcggcggcgc ggcttttgtc attggacccc
     4081 gcgaagaagc cgtagtgaac attgaagcga cttactcttt tgtttctgac acacctgatt
     4141 tttggcggcg accggaacaa aaataccctg agcatggcca gcgttttaca ggcgacccgg
     4201 cttactttaa acatgttgaa tctgctggca gggtgatgct ggaagaactg ggaatgaaag
     4261 cctctgactt cacctacgcg gtcttccacc agcccaaccc gagattcccg cttaaggccg
     4321 ggaaggatct gggcttcacc gaggagcaga taaaacccgg gctgctggta ccggtcatcg
     4381 gcaataccta cgctggcgca tcgatggtcg gcctaaccgc cattctggat attgccaagc
     4441 ctggcgaccg tattttgcag gtttcatatg gttccggcgc gggatcagac gccttctatt
     4501 tggaggtgac agaaaagatc ggcgaaaggc gtgaactggc cccttcgacc gctgattatc
     4561 tcgcccgccg caccgagatc aattacgcca cctacgcccg cttccgcggc aagatcgata
     4621 tgggatagga gccagacatg acagaagtgg tcattgcggg cattggtcaa attcccgttg
     4681 gagaacatta cacgctcagt ctgcggtcga tgggcgttca ggcgctcacg cgggcgttaa
     4741 aagacagcgg cgggttgaaa cctgacgcgc tgtatatcgg caacctgctt tcttccatga
     4801 tctcgcacca atcaaacctg ggcgcgctgt ttaccgatta cggcaggctg gatagcattg
     4861 aagccttcac caccgaagcg gcgggcgctt caggcgcggc ggctctgcgc ctgggggtgc
     4921 tggccatcct cagcggtcag gcggacaccg tggcggtgct gggggtcgaa aaatggagcg
     4981 acctggtgcg agcggatgca gaagcgatga ttactcagga gctggattat gattacgaat
     5041 caatacccgg gctcaccagc accagccagg cagcgcttat tcagcagcgg tacttacacg
     5101 agcacgacct ggagcaaaac gcgctgagcg gtttcgcgtt actggcccac gccaacgctg
     5161 tccataatcc tttcgcgttc tatcgaaacc ctctgacgga agagggatac gcgcgagcag
     5221 ggatgctaag cgaccctatc accatctttg atggcgccac ctacgcggat ggcgctgctg
     5281 cgctcgttct cacccggcgc gagctgttgc cgaggggata cgcgcaccca gttatcagcg
     5341 tgatgggttc tggtgccagc agcgacgcgc tctcctggca tgaccggaca gagccgatgt
     5401 catttaccgc tgccgttcgc gcgattgatc gggctgttca gcaggcgggc ttgtctaaag
     5461 agcagataga cctgtttgaa ttggacgacg ctttttcaat ctacgccgcg ctgaccctgg
     5521 aaggtgtggg tctggcggat aagggcgcgg cctggaagat ggcgcgagat ggtgaataca
     5581 acatcggtgg gcgcctgccc attctgacca tgggcggctg cctggcacgc ggcaatccca
     5641 ttggcgcgaa gggggtgtac caggtggtcg aggcggtgac ccagctgcgc ggagcagcgg
     5701 gagccaacca ggtgaagggc gcgcgcgctg ccctcagcct ggcgctgggc ggcccggctt
     5761 ccaccgcgat cgcgcatgtg ttgaagagag aaagtgaata gcaaacagct atgcgtgata
     5821 gaaggagtgt tctccgccgg gaaagtgaga ttccgctcgc caacccctgc ccaaacttga
     5881 tagtatttga tagatttcaa ctcataaact tatgcgtaca ccttgaagga aaaggtagca
     5941 aaaggagtga aaaatggaaa acccgcgata ttggcgcttg aaaaaacaac gctacagcct
     6001 gctgggtgaa gtttgcccgc actgtgattc aaagatcttc ccaccgcgag atat
//
LOCUS       DAOTLW010000069         5984 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_12160_length_5984_cov_9.232923, whole genome shotgun sequence.
ACCESSION   DAOTLW010000069 DAOTLW010000000
VERSION     DAOTLW010000069.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5984)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5984
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_12160_length_5984_cov_9.232923"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="P5516_08240"
     CDS             <1..759
                     /locus_tag="P5516_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRT92459.1"
                     /translation="ILDYFAGSGTTGHAVINLNREDGGRRKFILVEMADYFDTVLLPR
                     LKKVTFSPEWKDGKPKRVATAEEAERGPLIIKVIRLESYEDALNNLLPSEESSGQVAL
                     QFEDYLLKYMLQWETRKSETLLNVEKISKPFDYCLHIYRDGETRVQKVDLPETFNYLI
                     GLDVAKRQVLDNNGRYYLIYRGATREGKSTVVIWRETAGWTEEDYKRDRDFVAKHKFT
                     EGADVIYVNGDSLIPGAQALEGVFKAKMFAGVEG"
     gene            762..2525
                     /locus_tag="P5516_08245"
     CDS             762..2525
                     /locus_tag="P5516_08245"
                     /inference="COORDINATES: protein motif:HMM:NF015210.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF262 domain-containing protein"
                     /protein_id="HRT92460.1"
                     /translation="MAEVLFKKVDYILKKLVEDISMGEIGLPDIQRPFVWSMTKVRDL
                     FDSMYRGFPIGYLLFWENGSSDTHRTIGAGPKQKVARLLIVDGQQRLTSLYAVMKAVP
                     VITKNFKSQRIKIAFHPLSEKFEVSNAAIEKDAEWIPDISILWQPDTKPHAFKRKFLE
                     KLGKRRPLTAEEEDIIHERIDRLIKLEEYPLTALEISSSVDEDKVSEIFVRINSKGEA
                     LNQANFILTLMSVFWDEGRKELEEFCRRSKAPSPDGSPSPFNHYLRPNPDQLLRVSVA
                     LGFRRARLEHVYSLLRGKDLETRQFSDEQRVKQFALLQEAQVYALDLQNWHEFFKVLK
                     RAGYPSDQLISSQMAVLYTYALWLIGKRDFKVNLYRLREVMARWFFMATLTGRYTGSP
                     EARFEQDMALLRGANRADDFVRILDEQISAVLTKDYWEVTLPNELETAAARNTAQFAY
                     YATLCLLEARVLYSKMKVSELLDPTTKAKKTALERHHLFPRKYLQRIGVREKRLINQV
                     ANYALVEWSDNIKISDRAPRDYVPELEKRFASDELQQMYDWHGLPSNWYELDYEDFLK
                     ERRRRIALIIKFGFERLVVGA"
     gene            2522..5713
                     /locus_tag="P5516_08250"
     CDS             2522..5713
                     /locus_tag="P5516_08250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HRT92461.1"
                     /translation="MSNGSTQYQKLENRLLLLSWLNSLLGYEKNRELLEDMKQAEEGF
                     DAQGRSHIYYRLISRGSKLKISADDLARYDDNIRAHLAVINACRTQPITLRYFQYLAA
                     LYTEIVLDRLFNHKAQLLADLNAFVCQRNAKKLPSEPQDEPFTETDLTKLAYWMATGS
                     GKTLILHLNYYQFLHYNKEPLDNILLVTPNEGLSEQHLAELAASGIPARRFDLNHGGL
                     WSDSKHTVQVIEITKLVEEKRGGGVSVPVEAFEGRNLIFVDEGHKGSGGEAWRKYRDA
                     LGETGFTFEYSATFGQALSAARNDPLTAEYGKAIVFDYSYRYFYGDGYGKDFRILNLQ
                     DETDEAKTDLLLLGNLLSFYEQQCLFDEQAEILRPYNLEKPLWVFVGSTVNAVYSENK
                     QKRSDVLTVVRFLHRVLANRDWAVKGIEELLAGKSGLVTPDGIDLFAGRFPYVRETGL
                     TAEALYDDILRRVFHSPAGGGLHVCDIKSAAGELGLRASGAEEYFGLIYIGDTAEFKK
                     LVEADGAGITLEEDAIASSLFTDINHPDTPINILIGAKKFMEGWNSWRVSNMGLLNIG
                     RREGSQIIQLFGRGVRLRGKNHSLKRSAALPGNHPPYIKLLETLNIFAVRANYMAQFR
                     DYLEREGVETEPLVELPLPIWTNEQMLQRGLVLPRPESGRDFATETILLLTPDENIRL
                     RVDLSVKVQVLESGAVGLQDARAQSEYKATTIPSESLTLVDWEQAYLELLDYKARKGW
                     SNLVIRPETLRAILEKVPYTLKASEAVFRPRTFTDRTRLQEAVTAILRKYLDIFYRRA
                     HERWDSQNLIYRPLDLDDPNLAFNRPSVQEKKAAYIVRVPRSHSDLVTAIKKLCEDMD
                     KLIKEENSGLPRLYFDRSIYLPLFLKKGSVLTADPPLLEESEERFVRDLKDYWEAEKD
                     KSLAGKEIFLLRNLSRGKGVGFFEESGFYPDFILWIVDEKKQRIVFIEPHGMLHAKAY
                     EHDEKASLWERLRELGKEVGRRSGRQDITLDSFIISATPYDDLYKRYDDGSWDRDKFA
                     KHHILFQERNQDYDYLCKIFDGYCTNRQISGLK"
BASE COUNT         1517 a         1539 c         1553 g         1375 t
ORIGIN      
        1 atcctcgact acttcgccgg ctccggcacc accggccacg ccgtcattaa cctcaatcgc
       61 gaggacggcg ggcggcgcaa gttcatcctg gtggagatgg cggattactt tgataccgtt
      121 ctcctgccgc gtttgaagaa agtcaccttc tcgccggagt ggaaggacgg caagcccaaa
      181 cgcgtggcca cggcagaaga agccgaacgg ggtccgctta tcatcaaagt aatccgcctg
      241 gaatcctatg aagacgccct caacaatcta ttaccttccg aagaaagcag tggacaggtg
      301 gcgctgcagt ttgaggacta tttactcaag tacatgctcc agtgggagac gcgcaaaagc
      361 gaaaccctgc tgaatgtgga aaaaattagc aagccctttg actactgctt gcacatatac
      421 cgcgatggcg agactcgtgt ccagaaagtg gaccttcccg agaccttcaa ctatctcatc
      481 gggctggacg tggcaaagcg gcaggtgctg gacaataacg gccgttacta cctgatttat
      541 cgcggcgcga cgcgcgaagg caagagcacc gtggtcatct ggcgcgaaac cgcaggctgg
      601 acagaggaag attacaaacg cgaccgtgac tttgtggcca agcacaaatt taccgagggt
      661 gcggacgtta tctatgttaa cggcgattcg ctcatccccg gtgctcaggc gctggaaggt
      721 gtgttcaagg caaaaatgtt tgcaggcgtg gagggatagg tatggcagaa gtacttttca
      781 agaaggttga ttatatcctt aagaaactgg ttgaagatat atctatggga gaaatcgggc
      841 ttcctgacat tcagcgtccc tttgtttggt cgatgaccaa ggtacgcgat ttgtttgact
      901 cgatgtatcg cggctttccc attggctatc ttctcttctg ggaaaatggt tcttctgata
      961 cccaccgaac gattggtgct ggtccgaaac aaaaggtggc gcgtctgctc atcgtagatg
     1021 gccagcaacg gttgacctca ttatacgcag tcatgaaagc cgtgcccgtg attacaaaaa
     1081 acttcaagtc ccagcggata aaaatagctt ttcatccgct gagtgagaaa tttgaagtct
     1141 ctaatgcagc gattgagaag gacgcagagt ggatcccgga cattagcatt ttatggcagc
     1201 ccgatacgaa accgcatgct ttcaagagaa aattccttga gaagttagga aaacggcgcc
     1261 ccttgaccgc cgaagaagaa gatataattc atgagagaat tgaccgactg atcaaactcg
     1321 aagaatatcc actcaccgcg ctggagattt cttcttctgt cgatgaagac aaggtctcag
     1381 agatattcgt gcgtatcaat agtaaagggg aagcgcttaa ccaggccaat tttattctta
     1441 ccttgatgtc cgtattctgg gacgagggtc gcaaagagct ggaagaattt tgtcggcgca
     1501 gcaaagcacc ctccccggat ggcagtcctt caccttttaa tcattatttg cgcccaaatc
     1561 ctgaccagct gctgcgcgta agcgtggcac tggggtttcg ccgcgcacgc ttagagcatg
     1621 tttattccct gcttcgaggg aaggacctgg aaacgcggca attctcagat gaacagcgcg
     1681 tgaagcagtt tgccttattg caagaggctc aggtatacgc tttggacctt caaaactggc
     1741 atgagttctt taaggtgctg aaaagggcag gatacccctc cgatcaactc atctcctcgc
     1801 aaatggctgt tctttacacc tatgcattgt ggctcatagg aaagcgagac ttcaaagtaa
     1861 atttgtaccg cttgcgtgaa gtgatggcgc gctggttctt tatggcaacg ctcacggggc
     1921 gttatacagg ttcgcccgaa gcgcggtttg aacaggatat ggccctactg cgcggcgcaa
     1981 acagagccga tgactttgtc cgcattctag atgagcaaat tagcgccgtc ttgacaaaag
     2041 actattggga agtaacttta cccaacgaat tggaaaccgc tgctgccagg aacactgcac
     2101 aatttgccta ctatgccacg ctttgtctgt tggaagcgcg ggtcttgtat tcgaagatga
     2161 aggtgtccga gttactggat cctaccacga aggccaaaaa gactgctttg gaacgtcatc
     2221 atctcttccc ccgcaaatac ttgcaacgaa taggggttcg agaaaagcgt ctcattaacc
     2281 aggtagcaaa ctatgcttta gtggaatgga gcgacaatat caagatttcc gacagagctc
     2341 ccagggacta tgtgccggaa ttagaaaaac gatttgcttc cgatgaactt caacagatgt
     2401 atgattggca tggattgcct tcaaactggt acgaacttga ttacgaggat ttcctcaagg
     2461 aacgaaggag acgaattgcg cttattataa aattcggttt tgagaggttg gtggttggcg
     2521 catgagcaac gggtcaaccc aataccaaaa actcgaaaac cgcctcctcc tgctttcctg
     2581 gctcaacagc ctgttgggtt atgagaaaaa ccgtgaactg ctggaggaca tgaaacaggc
     2641 tgaggaaggt tttgatgcgc aggggcgcag ccatatttac taccgcctca tctcgcgcgg
     2701 cagcaagctc aaaatctctg ccgatgacct tgcacgctat gacgacaata ttcgcgcgca
     2761 cctagcagtc attaacgcct gccgcaccca gcccattacc ctccgctatt ttcaatacct
     2821 ggcagcgctg tacacggaaa tcgtcctcga ccgcctgttc aaccacaaag cgcaactcct
     2881 ggctgatctg aacgcctttg tgtgccagcg caacgcaaag aaattgccca gcgagccgca
     2941 ggacgagccc ttcaccgaaa ccgacctgac caagctggct tactggatgg ctacaggtag
     3001 cggcaagacg ctgattctgc atcttaacta ctatcaattc ctgcactaca acaaggagcc
     3061 gctcgataac atccttctgg tcacgcccaa cgagggcctg agtgagcagc acctggccga
     3121 acttgccgct tccggtattc ccgcccgccg cttcgatctc aaccacggcg gcttgtggag
     3181 cgacagtaaa cacaccgtgc aggttatcga aatcactaaa cttgttgaag aaaagcgcgg
     3241 tggtggggtt agcgtgccgg tagaggcatt cgagggccgt aatctgatct ttgtggacga
     3301 agggcacaaa ggctccggcg gcgaagcttg gcgcaagtac cgcgatgcac ttggcgaaac
     3361 aggtttcacc tttgaataca gcgcaacctt tgggcaagct ctctcggcgg cgcgcaacga
     3421 tccgcttact gccgaatacg gcaaggccat cgtctttgat tactcctacc ggtactttta
     3481 cggggatggc tacggcaagg actttcgtat cctcaacctg caggatgaga ccgacgaggc
     3541 caaaaccgat ctgctccttc tgggcaacct gctctccttc tatgagcaac agtgcctgtt
     3601 tgatgaacaa gccgagatcc tgcgtccata caatttggaa aagccgctct gggtgtttgt
     3661 cggtagcacg gtcaacgctg tctatagcga gaacaaacag aagcgcagcg atgtgctaac
     3721 tgtggtgcgt tttctccatc gagtgctggc aaaccgcgac tgggcggtca aaggcatcga
     3781 agaactcctg gcgggaaaaa gcggtctcgt tacgccagat gggatagacc tgttcgccgg
     3841 gcgcttcccg tacgtgcgtg aaactggcct taccgccgaa gccctatacg atgacatcct
     3901 gcggcgggtc tttcactccc cggccggtgg cggtctgcac gtgtgcgaca ttaaaagtgc
     3961 cgctggcgag ctggggttaa gggcatccgg cgccgaggag tatttcggtc tcatctacat
     4021 tggcgacacc gcggaattca aaaaactggt ggaagccgat ggcgccggca tcacactgga
     4081 ggaagacgct atcgcctcct cgctctttac ggatatcaac caccccgaca cgccgattaa
     4141 tattcttatt ggtgccaaga aattcatgga aggctggaat tcctggcgcg tctccaacat
     4201 gggacttctc aacattggac gacgggaagg ttcacaaatc atccaactct tcgggcgcgg
     4261 tgtacgcttg cgcgggaaaa accattctct aaagcgtagt gctgcccttc cgggcaatca
     4321 tccaccgtat atcaaactgc tggagacact caacatcttt gctgtgcgcg ctaattatat
     4381 ggctcaattc cgtgattatc ttgaacgaga aggggtagag accgagccgc tggttgaact
     4441 accgctccct atatggacca acgaacagat gcttcagcgc gggctggtgc taccgcgtcc
     4501 tgagagcggc cgtgactttg ctaccgagac gatactcctg ctcacccctg atgaaaacat
     4561 ccgcctgagg gtggatttat cggtcaaagt gcaggtactg gaaagcggcg cggtgggcct
     4621 gcaggacgct cgcgcccaaa gcgaatataa ggcgaccact atcccgtcag aaagtctgac
     4681 attggtggat tgggaacagg cctacctcga gctgctagac tacaaggcgc gcaagggctg
     4741 gagcaacctg gtcattcgtc ccgaaaccct gcgcgctatc ctagaaaaag ttccctatac
     4801 tctgaaggca agtgaagccg tgttccgtcc tcgtaccttc actgaccgca cccgtttgca
     4861 agaagcggtc acagccattc tgcgcaagta cctggacatc ttctaccgcc gagctcacga
     4921 gcgctgggac agccaaaact tgatctatcg ccccctcgac cttgatgatc ctaacctggc
     4981 tttcaaccgt ccgagcgtgc aggaaaagaa agccgcctac atcgtgcgcg taccgcgttc
     5041 acacagcgac ctagttaccg ccatcaaaaa actgtgcgaa gatatggata aacttatcaa
     5101 agaagaaaac agcggcttgc cgcgcctcta cttcgaccgg agcatctatc tgcctctgtt
     5161 tctcaaaaaa ggcagtgtgc tcactgctga cccaccgctg ctggaagaga gtgaagaacg
     5221 cttcgtccgt gacctgaagg actactggga ggctgaaaag gataagtcgc tggcgggaaa
     5281 ggaaatcttc ctcctgcgaa acttgagcag aggaaagggc gtgggtttct ttgaagagag
     5341 cggtttctac cctgacttta tcttgtggat tgtggatgag aaaaaacagc gcatcgtctt
     5401 tatcgagccg cacggcatgt tacacgctaa ggcttacgag cacgatgaaa aagccagtct
     5461 gtgggagcgc ttacgcgagc taggaaagga ggtagggcga agaagcggtc gacaggatat
     5521 tacgctagat tcattcatta tctcagctac gccctacgat gacttataca agcgctacga
     5581 tgatggcagt tgggaccgag acaagtttgc taaacatcat atcttgttcc aggaacggaa
     5641 tcaggactat gactatctct gtaaaatctt cgatggctat tgcaccaacc ggcagatttc
     5701 agggctaaag taattgtgca tgaactgctg caccttaaag tgccaaacca taacctgtta
     5761 ttcaaagctc ttcttaaagc gtacttagac agatatcggt agtataaaag gcccttggga
     5821 aatactccct gggcctttta ctattaagtg gagattaatg cttatgactt tttggttgag
     5881 cctcttttgc caggccttat acttcgtctg accagtttga ttacaggtga agcaatccta
     5941 tgctgatttt ctatatccat ttgtgctagt tttacataac gcga
//
LOCUS       DAOTLW010000070         5623 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_13016_length_5623_cov_8.264907, whole genome shotgun sequence.
ACCESSION   DAOTLW010000070 DAOTLW010000000
VERSION     DAOTLW010000070.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5623)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5623
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_13016_length_5623_cov_8.264907"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..733)
                     /locus_tag="P5516_08255"
     CDS             complement(<1..733)
                     /locus_tag="P5516_08255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT92462.1"
                     /translation="MNILYVQAGQAVGGSKESLYQILRSLPDEFARQVVLERGSDPGF
                     NERARRYASAVHLLPLPTWQQYRRRTLAERARAPLADAWRLLRCALAAFKIARVIRQE
                     RIDLVHTNNSMSPSGALAARLTSTPHIWHVRESIGEHNQYPLLLGDLALRLMCRWSDT
                     IICNSDYTAEVFREMRLPVRVIPNGLELADFAPGAGTPRELSSLADLPPGEKRVGMVG
                     NLTTKMKRHDVFLDIAALVKAAAPRT"
     gene            complement(730..1620)
                     /locus_tag="P5516_08260"
     CDS             complement(730..1620)
                     /locus_tag="P5516_08260"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT92463.1"
                     /translation="MNLPTVSIVTPCYNAARFIRETIESVSGQRYPHLEHIVIDGGST
                     DGTLDILKAYPHLRWVSAPDRGQSHALNKGFAMARGELIGWLNADDTYAPGAVSTAVD
                     YLLQHPEAGMVCSDMWIVGENGKPLRLARGKPFDLVELLTENYVRQATVFIRRQLLNE
                     VGGVNESLHYCMDRALWLRVGVHHQVAYLPGIVSANFRFYWGSKTSLYTPGFVAEWLG
                     VLTDALDNPLYQPIPRAEVLKAIEKYQVRHQVALMRQAARQKDPRSTWRACAALLSRH
                     WRYLLRYPYLKTRALLAAKR"
     gene            complement(1627..2721)
                     /locus_tag="P5516_08265"
     CDS             complement(1627..2721)
                     /locus_tag="P5516_08265"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HRT92464.1"
                     /translation="MRNPPGSRATCVVHYPSLRVMAGNPYWAMLESGLKEAGITLLDE
                     PDAHKLRWLFKNRNRVDILHFHYFQFLYCNRGRSRARLVYVARFALFLLLARLLGYRT
                     VLTLHNLKPTHPLNPAWIDYLGHWLAANFTQRVIVHCHEARRLLAARYGRKNNVIEVA
                     HPHYLGYASGKPSKTEARKILRLPPRSPVFLFFGGVRRNKGLENLIQTFKEIEEREPT
                     LLIAGKPEPGAGYAEQLQSLAGKDPRIRFHFGYIPDEELALFFNASDIVVLPFSAILT
                     SSSTILAMSFARAVIAPRMGCLPELLEPGAGWLYEADSPGALLQTLGRSLKEDWRQAG
                     VTALERVASHTPAIFIRETLSCYGCTNSSA"
     gene            complement(2718..3968)
                     /locus_tag="P5516_08270"
     CDS             complement(2718..3968)
                     /locus_tag="P5516_08270"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HRT92465.1"
                     /translation="MNKKTTILFITAYYPPHHGGGFGLRCRDVAEGLRRRGHSLTILT
                     NRCALRGCCEHQSEERTLRLLALRPAGDNPLRQILFDRRELLQVARVVRQVQPRVIYL
                     WHMQNLSNAILPFLAGQPTPLILDDGGSELIYLSRLQKRGLYFYKNAGEPRLKTWLKR
                     LVKTGARLVSGGRIVPDWQWPEGMRVLFNSPSALEHARAQGGFARNASVVPSGLDIAR
                     FPFKAAPRIHTPVKIILPARIKEQKGCLDGILLVEELRRRGVPAQLLIIGEAQSAAYL
                     ALLRREVEARRLSGQVDIRPMAGQDEMSRLYREHDFCFFPSSFKTGFSRVPLEAMASG
                     CLLLTYGNEGSREVVQDGATGYLLPEGDVATAAERVHASTLDHAGYLRILSTARRQVE
                     ERYSLEDYLQKVEALLSRAGETPS"
     gene            complement(3965..4942)
                     /locus_tag="P5516_08275"
     CDS             complement(3965..4942)
                     /locus_tag="P5516_08275"
                     /inference="COORDINATES: protein motif:HMM:NF025025.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT92466.1"
                     /translation="MASIGKDNPLVSVIVPLRNRFNLVDEAVDAVYKQNYRPIELILV
                     DDCSSQAYRPKIQAHAGFTVAVIRHQENQGPGASRETGRQRATGAYLAYLDSDDLWHP
                     TFLEKMVLQLKEKPETGMCYCKTRQFSGADLNSGSLRPRNEQAFDAILPTLLFGRPWS
                     TGACLWTRRAADQIGPWSEGWTNEDIEYEIRAGIFGIQISHLPEVLCYERETDDPQQL
                     SQATHKKALLQKFSSIKAMAITVLENKHALEESTRVLFVKKKMRPMVTDLFQAEEYEK
                     VIELCKLMNKIVKPLSKAWLLTALLLASVRYRKFPGAAKIFQLGCHFLT"
     gene            complement(4912..>5623)
                     /locus_tag="P5516_08280"
     CDS             complement(4912..>5623)
                     /locus_tag="P5516_08280"
                     /inference="COORDINATES: protein motif:HMM:NF025877.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HRT92467.1"
                     /translation="VLAVGDAEFQKKCLGKMGNVASEEGRTVLFVSHNMAAIQQLCTK
                     SILMNSGRLELAGDSLKVVKKYLASNEEMAAEKKHAILARNEQENFQLSRVFFADETG
                     STQNSFMSGETITIHLEIESETIFRGLIVTIGINDENGTRLTVLHSDLSGVPLQVTGK
                     HTVVTCNIPNLLLLPGKYYLDIKIYRGKDVLLWVPNAKTLFVEPGNILKSGKLPPKEW
                     GGFFVLDNQWQASEKTTR"
BASE COUNT         1109 a         1606 c         1712 g         1196 t
ORIGIN      
        1 tggtgcgcgg cgcggcggct ttgaccagcg ccgcgatatc caggaaaaca tcatggcgtt
       61 tcattttcgt ggtgaggttg ccaaccatgc ctacccgttt ttcgcccggc ggaaggtcgg
      121 caaggctgct cagctcgcgg ggcgttcccg caccgggggc gaaatccgcc agctccaggc
      181 cgtttgggat gacgcgcacc gggaggcgca tttctctgaa aacttcagcg gtgtaatcag
      241 aattgcagat tatggtgtcg ctccaacggc acatcaggcg cagggcgagg tcgcccagga
      301 gcaaagggta ctggttgtgc tcgccgattg attctctcac atgccagatg tggggggtgg
      361 aggtgagccg cgccgccagc gcgcctgagg ggctcatgct gttgttggtg tgaaccaggt
      421 cgatccgctc ctggcggatg acccgcgcga ttttgaaggc ggccagcgcg caccgcagaa
      481 gccgccaggc gtccgccagc ggcgccctgg cgcgttcagc cagcgtgcgg cggcggtatt
      541 gctgccaggt gggcagcggc agcaggtgca ccgctgaggc gtagcggcgc gcgcgctcgt
      601 tgaaaccggg gtccgagccg cgttcaagca cgacctggcg cgcaaattca tcgggcaatg
      661 accggaggat ctggtaaaga ctttcctttg accctcccac agcctgcccc gcctgcacgt
      721 agaggatgtt catcgtttag ctgccaggag cgcccgcgtt ttcaggtaag ggtacctcaa
      781 caggtagcgc cagtgccggc tgaggagcgc ggcgcaggcg cgccaggtgg agcgcgggtc
      841 tttttgcctg gctgcctgcc gcataagcgc tacctggtgc cgcacctggt atttttcaat
      901 ggctttgagc acttcagcgc gtggaatggg ttgataaagc gggttatcca gcgcgtcggt
      961 gagcacgccc agccattccg ccacgaaccc gggtgtatac agcgaggtct tgctgcccca
     1021 atagaacctg aagttggcgc tgacgatccc cggcagatag gcgacctggt gatgaacgcc
     1081 caccctcaac cacaaggcgc ggtccatgca gtagtgcagc gattcattca cgccgcccac
     1141 ttcattgagc agctgccggc gaataaagac ggtcgcctgc cgcacgtagt tctcggttaa
     1201 cagctcgacc aggtcaaatg gtttgccgcg ggcgagccgc aggggctttc cattttcacc
     1261 gacgatccac atatcgctgc aaaccatgcc cgcttcggga tgttgaagga ggtaatcgac
     1321 cgcggtcgat accgcgccgg gggcgtaggt gtcatcggcg ttcagccagc cgatgagctc
     1381 gcctcgcgcc atggcgaatc ccttgttcag cgcgtgcgat tgccccctat cgggcgctga
     1441 gacccagcgc aaatgcgggt acgctttgag gatgtcaagc gtgccgtcgg tggagccgcc
     1501 gtctatgacg atgtgctcca aatgggggta acgctgcccg ctgacgctct cgatggtttc
     1561 acggatgaag cgggcggcgt tgtaacaggg ggtgacgatg ctgacggtgg gaaggttcat
     1621 gagcacttac gcagagctgt ttgtgcagcc ataacacgac agggtttcac ggatgaagat
     1681 ggccggggtg tggctggcaa ctcgctccag ggcggtgacc cccgcctggc gccaatcttc
     1741 tttcagcgac ctgccgagcg tttgcaataa agcgccgggg ctgtccgctt catacaacca
     1801 gccggcccct ggctccagca attcaggcag gcagcccatg cgcggggcga tgaccgcgcg
     1861 ggcaaacgac atcgccagga tcgttgagct ggaggtgagg atagcgctga agggcaaaac
     1921 gacgatgtcg ctcgcgttga aaaagagcgc cagctcctca tctggaatat agccaaaatg
     1981 gaacctgatg cgcgggtctt ttccagccag gctttgcagc tgctcggcgt acccagcgcc
     2041 gggctctggt tttccggcga tgaggagcgt gggctcgcgc tcttcgatct ctttgaaggt
     2101 ttgaatcagg ttttccagcc ctttgttgcg gcgcacgccg ccaaaaaaga ggaagaccgg
     2161 agagcgcggc ggcaggcgca aaattttgcg agcctctgtt ttggagggct tgccggaagc
     2221 atagccgaga tagtgtggat gagccacttc aatgacgttg tttttcctgc cataacgcgc
     2281 ggctaaaagc cgcctggctt catggcagtg aacgatgacg cgttgggtga aattcgccgc
     2341 cagccaatgc cccaggtaat cgatccaggc cgggttgagc ggatgggtgg gcttgaggtt
     2401 gtgcagggtc agcacggtgc ggtagccgag caggcgggcg agcagcagga agagcgcgaa
     2461 cctggcgaca taaacaaggc gcgcgcggct gcgcccgcgg ttgcaataca gaaattgaaa
     2521 ataatgaaag tgcaggatat cgaccctgtt tcgatttttg aacagccagc gcagtttgtg
     2581 cgcatccggc tcatcgagga gcgttattcc agcctcctta agccctgatt caagcatcgc
     2641 ccagtagggg ttgcccgcca tcacccgcag tgaaggataa tgcacaacgc aggttgccct
     2701 gctgccgggc gggtttctca tgacggggtt tctccagcgc ggctgagcag cgcttcgacc
     2761 ttctgcaggt aatcttccag tgaatacctt tcttccacct gtcggcgcgc ggtggaaagg
     2821 atgcgaaggt aacccgcgtg gtcgagggtg gacgcgtgaa cccgttccgc ggcggttgcc
     2881 acatctccct cgggcagcag gtagcccgtt gctccgtcct gcaccacttc ccgcgagcct
     2941 tcattcccat aggtgagcag caggcagccg ctggccatgg cttccagcgg cacgcgcgag
     3001 aagccggtct tgaaggacga ggggaagaaa cagaaatcat gctcgcggta aagccggctc
     3061 atttcatcct gccccgccat ggggcgaatg tccacctgcc cgctcaggcg gcgcgcctcc
     3121 acctcgcgcc gcagcagcgc caggtaggcc gcggactgcg cctcgccgat gatgagcagt
     3181 tgggcgggca cgccgcgccg gcgcagttcc tccaccagca ggatcccatc caggcagccc
     3241 ttctgctctt tgatgcgcgc cggcaggatg atcttcaccg gcgtgtgaat tcgcggcgcg
     3301 gctttgaacg ggaagcgcgc gatatccaac cccgagggga cgaccgaggc gttacgcgca
     3361 aaccctccct gcgcgcgggc gtgctcgagc gcggaagggc tgttgaagag cacgcgcatg
     3421 ccttcgggcc actgccaatc tggcacaatc cgcccgccgc tcaccagcct ggcgcccgtt
     3481 ttaaccaggc gcttaagcca ggtcttgagc cgcggctcgc ccgcgttctt gtagaagtac
     3541 aacccgcgtt tctgcagccg tgaaaggtaa atgagctccg agccgccgtc atcgaggatc
     3601 agcggcgtgg gctgcccggc gagaaagggc agaatcgcgt tcgacaggtt ctgcatgtgc
     3661 cagaggtaga tgacccgcgg ctgcacctgg cggacgacgc gcgccacttg caataattcg
     3721 cgccggtcaa acaggatctg ccgcagcggg ttgtctcccg ctggtctgag cgccagcagg
     3781 cgcaatgttc gctcctcgct ctggtgttca cagcagccgc gcagcgcgca gcggttggtg
     3841 aggatggtga gcgagtgacc gcgccggcgc agaccctccg ccacatcacg gcagcgcagg
     3901 ccgaaaccgc cgccgtgatg cggggggtaa taagcggtga tgaagaggat ggtggttttc
     3961 ttgttcaagt gaggaaatga catcccagct gaaagatttt cgcggcgccg gggaattttc
     4021 gataccgtac gcttgccagc aggagggcgg tgagcaacca tgctttcgag agaggcttca
     4081 cgattttgtt catcaactta caaagctcga ttactttctc gtattcctct gcttgaaaca
     4141 ggtcagtgac catagggcgc atcttttttt tcacgaataa taccctggtg gattcttcaa
     4201 gtgcgtgctt gttttcaagc acggttatgg ccattgcctt aattgaagaa aatttttgca
     4261 gcagcgcctt tttgtgggtt gcttgagaca actgctgggg atcgtccgtt tccctttcat
     4321 agcacagcac ttctggcagg tgggatattt gaatgccgaa gatgcctgcg cggatttcgt
     4381 actcaatgtc ctcgttcgtc cagccttcag accatggacc gatttggtca gcagccctcc
     4441 tggtccataa acaggcgcct gtgctccagg ggcgcccaaa gagtaatgtg ggcaggatgg
     4501 cgtcaaatgc ctgttcgttg cgaggacgca aagaccctga gttgaggtcc gcgccactga
     4561 actgcctggt cttgcagtag cacatcccgg tttcgggctt ctctttgagt tgcaagacca
     4621 ttttttccag gaaggttgga tgccacaggt catcagaatc aaggtaggcg aggtaggcac
     4681 cagtcgctcg ctgccggcca gtttcccgcg acgcgccggg tccctggttc tcttggtgcc
     4741 gaatcacagc aaccgtgaaa cccgcgtgcg cttgaatttt tggcctgtag gcctggctgg
     4801 agcaatcatc caccaggatc aactcaattg ggcggtaatt ctgcttgtaa accgcatcaa
     4861 cggcttcatc gaccaggtta aaacggttgc gaagggggac aatgaccgaa actagcgggt
     4921 tgtcttttcc gatgcttgcc attgattgtc caaaacgaag aatccgcccc attcctttgg
     4981 gggcagctta ccgcttttca gaatattccc tggctcaacg aacagcgttt tcgcgttggg
     5041 cacccaaagg agcacatcct ttccacgata gatttttata tccaggtaat atttacccgg
     5101 cagcaggagc aggttcggaa tgttgcaggt tactacggta tgcttgccgg taacttggag
     5161 ggggacaccg gagagatcac tatgcaggac ggtcaacctt gtgccatttt cgtcattgat
     5221 gccgattgtg acgatcaaac cgcgaaaaat ggtttccgat tcgatctcca ggtgaatggt
     5281 gatggtttct cctgacataa aactgttttg agttgacccg gtttcatcag caaagaacac
     5341 tctggataat tgaaaatttt cttgttcatt cctggccaag atggcatgtt ttttctctgc
     5401 tgccatttct tcattggaag ccaggtattt tttcacgact ttcagagaat cgccagccag
     5461 ttcaagcctg ccggagttca tcaagatcga ttttgtgcat aactgctgaa tcgccgccat
     5521 attatggctc acaaacagca ccgtccgccc ctcctcgctg gccacgttac ccatcttgcc
     5581 gaggcatttt ttttgaaact cagcgtcgcc caccgccagc act
//
LOCUS       DAOTLW010000071         5208 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_14150_length_5208_cov_10.832913, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000071 DAOTLW010000000
VERSION     DAOTLW010000071.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5208)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5208
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14150_length_5208_cov_10.832913"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..321
                     /locus_tag="P5516_08285"
     CDS             <1..321
                     /locus_tag="P5516_08285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92468.1"
                     /translation="EAQQITGWFTIPIVIPLWFTNVFMFNPNGALSVAMSLFPVTAPI
                     ALPFRAAFTTVPQWQIILSITLLCLLAAFALWLSGRIFRLGMLRYGKRVRLREAFSKS
                     GSKP"
     gene            318..1550
                     /locus_tag="P5516_08290"
     CDS             318..1550
                     /locus_tag="P5516_08290"
                     /inference="COORDINATES: protein motif:HMM:NF024091.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92469.1"
                     /translation="MKKTLLILKNELITALTRKSFLITLVLLPLTSFIILLVISGLQK
                     SGQDTSAMLENLFVPASLSSAEGFVDNSGLMKEIPDEIANALIRYNTEEDARAALNAG
                     LISAYFIVPDDYLEKGDVFYIRPDFNPMGSTQQSSSIKSLIAFNLSGGNPALAYRLQD
                     PINAIEVSLSNQEQRDSDHWLTFFLPYIITFLFYIVILTSSSLLLNSISTEKQNRVME
                     ILLTSLTPSQVLTGKIIALGLTGLLQTVVWLGAGLLMLRYSGRAFALSAAFQLPLSIM
                     AWGVLFFIFGYAVFASLMAGIGALVPNLREGSQLTTVVILPMIIPLVFISALINTPNS
                     PLSVFLSMFPLTAPVSMMTRLSATQVPFWQASLALLLLALTAVFLVRASASLFKAQNL
                     LTGQTFTVAGFLKALAGK"
     gene            1601..3922
                     /locus_tag="P5516_08295"
     CDS             1601..3922
                     /locus_tag="P5516_08295"
                     /inference="COORDINATES: protein motif:HMM:NF016289.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HRT92470.1"
                     /translation="MQRKKLVIILVLGFLMAAVAETLLLVSMINTRNKTSADRSGQPQ
                     QLDATIPSSTEPALGVPDGIGPALVEVYTKQNALAAISTERMFARLTELTRIQPHSGW
                     RGAGTTGEKEAFDYLESRLTELEWLTSLGMSSERERFNIFLGTEVHQSSLFLTLGAHT
                     YEVPADATRGSRDNPAAALRMDSDGFFNPPYEDPVEVTGFPLLIPDAAALNSLEKRSY
                     QNEVFLVDYALVDTENPQALSNALSLLRLNPAAIVLITRFSNTGGQTHGTFAGDGGGV
                     LQRVETPGALPLLFIEFENLSPLGIHDWDEMNVLSQARVIWDTDVLNPAPSGNLVVHI
                     PGKYSDKPILLSAHIDSPNSPGALDNGSGSVILLEILTVLNDLKIQPENDLFFAWFGS
                     EEAGLYGSAAFTTMHSDLLGKLQANIQIDCLTRPLDGLPAGIMPMYSHISTSDLNTDP
                     FKSFMDDTGAELGLELKSIFYEFASDNGSLSAFNIPNTNLIYQSMEMENYPGGVWVSG
                     HLHDPYDTVELAQEMEAELRDMTRLALAAALSGHAQIDFVQHDPQKRAVFLANHTEAP
                     HMTPSGLSRFSKSLIRAGYGITVIPYGEPLSTQHISGADLVVVLPAYDYPLTDAYPAA
                     YDTSWTAVEAAILNEYVEHGGKLLVVNSANRLKLFNRVAEPNEDWSDLNILTNQWGVN
                     FTTAGVDETTAAVTADGWLQQVSMVNLNPQNAVTFAVTSGEVLAGSRSRAHIAQLKMR
                     SGTVFIFSDLSMLGDYGDGLFNPKLVQALAAWE"
     gene            complement(3944..5089)
                     /locus_tag="P5516_08300"
     CDS             complement(3944..5089)
                     /locus_tag="P5516_08300"
                     /EC_number="6.5.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF013319.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HRT92471.1"
                     /translation="MPRLILTERLPIKLWIDVIDEGALAQARNLANLPFAYKWIAIMP
                     DCHEGYGMPIGGVLAADGMIVPNAVGVDIGCGMLAARTSLHTLEKAALQQIRNRIKQD
                     VPLGFEHHKTAQPWTGFDRAPDIPIIQQELDSARRQLGTLGSGNHFIEIQQGSDGNIW
                     VMIHSGSRNFGLKTATAYHHTARALCTRMGYSLPDRDLAFLPLDTREGMDYFTAMNYC
                     CEFARANRARMMERVMQILADETGANRLEEINIHHNYAALERHYGREVMVHRKGATKA
                     SLNLTGIIPGSMGSNSYIVKGLGNPESFESCAHGAGRRMGRKQAQRSLDLAEEQKKME
                     GVAHDLRTRDSLDEAPGAYKDIDEVMRLQQDLVSITVTLTPLGSIKG"
BASE COUNT         1133 a         1510 c         1317 g         1248 t
ORIGIN      
        1 gaagcgcaac aaataaccgg ttggttcacc attcccatcg ttatcccgtt atggttcacg
       61 aacgttttta tgttcaaccc caacggcgcg ctttcggttg caatgagcct tttcccggta
      121 acagcgccaa tcgcgctgcc tttccgcgcg gcgttcacca ccgtacctca atggcaaata
      181 atcttatcta tcaccctgct gtgcctgctg gccgcctttg cgctctggct ttccgggaga
      241 atcttccgcc tgggaatgct gcgttatgga aaacgcgtac gcctgcgcga agccttctcc
      301 aagagcggaa gtaaaccatg aaaaagaccc ttctgatatt aaagaacgaa ctcatcactg
      361 ctcttacccg caaatccttc ctgatcactt tggttttatt gccccttacc agttttatca
      421 ttttgctggt gatatcaggt ctgcagaaat ctgggcagga caccagcgca atgctggaaa
      481 atttgtttgt tcccgcgtcg ctgtcttcag ctgaaggttt cgtggataat agcgggctga
      541 tgaaagagat accggatgag attgccaatg cgcttatccg ctacaacacg gaagaagacg
      601 cgcgggctgc gctcaacgcg ggactcatca gcgcttattt catcgttccc gatgattacc
      661 tggagaaagg tgatgttttc tatatccgtc cggattttaa cccgatggga agcacgcagc
      721 agtcttcctc gattaagtcc ctcatcgcct tcaacctcag cggcggtaat cctgccctgg
      781 cttacaggct gcaggacccc atcaacgcta tcgaagtgag cctttctaac caggaacaac
      841 gagatagtga ccactggctc acattctttt taccgtacat catcaccttt cttttttata
      901 tcgtcattct cacctcttcc tcgctgctgt taaacagtat cagcactgaa aaacaaaacc
      961 gggtaatgga aatcttgttg acctcactca cccccagcca ggtactcacc ggcaagatca
     1021 tcgccctggg gttgacaggg ttgctgcaaa cggtggtgtg gctgggcgcg gggctgctga
     1081 tgctgcgtta ctctggtcgc gcttttgcgc tcagcgcagc gtttcagctg ccattgtcga
     1141 taatggcctg gggcgtgctg tttttcattt tcgggtacgc ggtttttgcc agtctcatgg
     1201 cgggtatcgg cgcgcttgtt cccaacttgc gcgagggatc acaattaacc accgtggtca
     1261 tcctgccaat gatcatccca ctcgtgttca tcagcgcgct gattaacacc ccaaacagcc
     1321 cgctctccgt atttctcagc atgttcccgc tcactgctcc tgtaagcatg atgacccggc
     1381 tttccgccac gcaggtgccg ttctggcagg cgagcctggc cttgttatta ctggcactta
     1441 ccgctgtgtt cctggtgcgt gccagtgctt cgctttttaa ggctcaaaac ctcttgaccg
     1501 gtcaaacctt tactgtagca gggtttctca aagccctggc agggaagtaa ttcacaccat
     1561 ccttcatcgt cttcatcacg cctttttgaa ggaggtttgc gtgcaacgca aaaaactggt
     1621 cattatcctg gtccttggct tcctgatggc agccgttgcc gaaaccttgc tcttggtttc
     1681 tatgatcaac acccgcaaca aaacaagcgc ggaccgcagt gggcaaccac aacagctcga
     1741 tgccacaatc ccttcctcga cagaacctgc gcttggcgtt ccagacggga ttggacctgc
     1801 cttagtggaa gtttatacaa aacaaaacgc cctggcagcc atttctactg aacgcatgtt
     1861 cgcgcgcctc acagaactta cccgcatcca gccgcattca ggctggcgcg gcgcgggtac
     1921 caccggggaa aaagaagcct tcgactacct ggaatccagg cttacggaat tggaatggct
     1981 cacgagcctg ggaatgtcat ccgaaagaga gcggttcaac atcttcctgg ggaccgaagt
     2041 tcatcaatcc tctcttttct tgactttggg agcgcatacc tatgaggttc ccgctgacgc
     2101 aacccgcggc agccgcgaca accccgctgc agccttgcgc atggactctg acggcttttt
     2161 taatccaccc tatgaagacc ctgttgaagt cacaggtttc ccgctgctta ttccagatgc
     2221 cgccgcgctg aacagcctgg aaaaaaggag ttatcaaaac gaggtcttcc tggttgatta
     2281 cgcgctggtc gatacagaga acccccaggc gctgagcaat gccctctctc tgctgagatt
     2341 gaaccccgct gccattgtgt tgatcaccag gttttccaac actggcgggc aaacgcacgg
     2401 aaccttcgcc ggggatggcg gcggcgtgct gcaacgggtg gagaccccgg gcgccctgcc
     2461 cctcctgttt attgagttcg aaaacctctc cccgttggga atccatgatt gggatgaaat
     2521 gaatgttctc tcgcaggcgc gcgtcatttg ggatacagat gtgctcaacc ctgctccatc
     2581 cggtaatctt gtggtgcaca tccccggtaa atattcagat aagccgatcc tgctttcagc
     2641 ccatatcgac agcccaaatt cccccggcgc cctggacaat ggcagcggct cggtcattct
     2701 gcttgaaatt ctcactgtgc tcaacgactt gaagatccaa cctgagaatg acctgttttt
     2761 cgcctggttc ggcagcgaag aagcagggtt gtacggctct gccgccttca ccaccatgca
     2821 ttctgacctg ctcggcaaac ttcaggcgaa catccagatt gattgcctga cgcgcccgct
     2881 cgatggcctg cccgccggca ttatgcccat gtacagccat atcagcacct ccgacctgaa
     2941 caccgatccc ttcaaatcct ttatggatga tacaggcgcg gagcttggac tggagctgaa
     3001 atccattttt tacgaatttg cttcagataa cggcagcctc agcgccttca atattcccaa
     3061 taccaatctc atttatcaat ccatggaaat ggaaaactac cctggtggag tttgggtctc
     3121 aggtcatctg catgaccctt acgatacggt ggaactggcg caggaaatgg aagccgagct
     3181 acgggatatg accaggttgg cgctggccgc tgccctcagc gggcacgcgc agatcgattt
     3241 tgtgcagcac gacccgcaga aacgggcggt ttttcttgcc aatcacacag aagccccgca
     3301 catgacgccc agcgggttgt caagattcag taagtcctta atccgcgccg ggtatggtat
     3361 cactgtcatt ccctatggcg aaccgttatc cacacagcat atcagcgggg cagacctggt
     3421 ggtggtgttg ccagcctacg attacccgct caccgatgcc taccctgcgg cgtatgatac
     3481 gagttggaca gctgtcgaag cagcgatcct caatgaatat gtggagcacg gcggcaagtt
     3541 gctggttgtc aacagcgcga accgcctgaa gctcttcaac cgggttgccg agccgaatga
     3601 agattggtcc gacctgaaca tcctgaccaa ccagtgggga gtgaacttta ccacggctgg
     3661 ggtggatgaa accacggcag ccgtcacggc ggatggctgg cttcagcagg tatcgatggt
     3721 caatctcaac ccgcaaaacg cagtgacctt tgcagtcact tcaggcgagg ttttggcagg
     3781 cagccgcagc cgggcgcaca tagcccaact caaaatgcgg agcggtacag tattcatctt
     3841 cagtgacctg agcatgctgg gtgattatgg agatgggttg ttcaacccga aactcgtaca
     3901 ggcgctggct gcctgggaat gatcgccctc ttgaagtcag cgcttaccct tttatagaac
     3961 ccagtggcgt gagcgtgact gtgatgctta ccaggtcctg ctgcaggcgc atcacttcgt
     4021 cgatgtcctt ataagccccg ggggcctcat ctaacgagtc ccgcgttcgc aggtcatgcg
     4081 ccacaccttc cattttcttt tgttcctctg ccaggtcaag cgagcgctgc gcctgcttgc
     4141 gccccatacg ccggccagca ccgtgcgcgc atgattcaaa gctctccggg tttcccagtc
     4201 ctttgacaat gtaactgttg gaacccattg acccggggat aatccccgtt aaattgagag
     4261 aggctttggt tgcccctttg cggtgcacca tcacttcacg accgtagtgc cgttcaagag
     4321 cagcgtaatt gtgatggata ttgatttctt ccagccggtt cgcgcctgtc tcatcagcga
     4381 gaatttgcat tacccgttcc atcattcgtg cgcggttggc tcgggcgaat tcgcagcagt
     4441 agttcatggc tgtaaaataa tccatccctt cgcgcgtatc caaaggcaaa aatgccaaat
     4501 cccggtcagg caggctgtaa cccatccggg tacacaacgc ccgggctgtg tggtggtagg
     4561 cggtcgccgt cttaagaccg aaattacgac tgccggaatg gatcatcacc caaatatttc
     4621 catcgctgcc ctgctgtatc tcaatgaaat gattaccgct gcccagtgtg cccagctgcc
     4681 tgcgcgcgct atcgagctct tgctggatga tggggatatc cggcgcgcga tcaaaccccg
     4741 tccagggttg cgcggtctta tggtgctcaa accccagcgg tacgtcctgc ttaatgcggt
     4801 ttcgaatttg ctggagagca gctttttcca gcgtatggag gctggtgcgc gctgccagca
     4861 tcccgcagcc gatatccacc cctaccgcgt tgggcacaat catcccatcc gcagccaaca
     4921 cgccgccgat gggcatcccg taaccttcgt ggcagtcagg catgatcgcg atccacttgt
     4981 aggcaaacgg cagattagcg aggttgcgcg cctgcgccaa cgctccttcg tcaatcacat
     5041 cgatccagag cttgataggc agtctttcag taaggataag cctgggcatg aaaacacctc
     5101 tctcccattg taacaaaaac ggcgaggaac aaagtgaata aggtataatg agggtgaagc
     5161 ccccgtagct caattggaca gagtgcctga cttcgaatca gtaggttg
//
LOCUS       DAOTLW010000072         5128 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_14362_length_5128_cov_9.698798, whole genome shotgun sequence.
ACCESSION   DAOTLW010000072 DAOTLW010000000
VERSION     DAOTLW010000072.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5128)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5128
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14362_length_5128_cov_9.698798"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="P5516_08305"
     CDS             <1..1074
                     /locus_tag="P5516_08305"
                     /inference="COORDINATES: protein motif:HMM:TIGR01451.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92472.1"
                     /translation="AFTWNPAHADDPYTQPAINKTFSDIVIIPGVTTQLTVKVFNENQ
                     FGLVDIAYTDVMPPNIKIVTPAVASNSCGGTVTAVPGTNTFSISGGSVPPKTTSVPAE
                     CSLVLNVTSTVAGTHINTIHAGDLSARDADRALPVLHNEYPASATLQVLVVQPPSLSK
                     IFNPTTIFVGEVSRLTITIRNNDLLNDLHETTFTDTLPAGVVLAPTVNPVLTGCGTGT
                     VTAVSGTNTITLNNATVTKNSTCSVAVNVTSSTQSDTPYVNTIPAGPGSGAISTREGV
                     TNATAASASLYVQNVGIAKSFSPTSIVAGATSTLTITLRNPTGTAYTGASISDTLPAG
                     LIIAPAGGSTTCGGGDYRLPRWG"
     gene            complement(1153..1560)
                     /locus_tag="P5516_08310"
     CDS             complement(1153..1560)
                     /locus_tag="P5516_08310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92473.1"
                     /translation="MVQLEFLGIDPPTNVTVVAVYETVPPHWGVAGTPETVTLAGRVS
                     VNVTSVRSPFTSVRLFLRVIVTVDVPPVGMDAGLKAFVPVSQRVIARLAVTAEGLVIP
                     WSVVREFGGMVFVKVAAGASGKVVTATVMVQVP"
     gene            1748..>3669
                     /locus_tag="P5516_08315"
     CDS             1748..>3669
                     /locus_tag="P5516_08315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92474.1"
                     /translation="MRLRIRLTSPTDIGISGINFTDTLPAGMRITNSTPASNACGGTL
                     TSITGTNQITLTNGAIAAPDTACNIDVYVTADDSMSYTNTIPVGAITTAELRSNTSAA
                     SATLVVSGFTMSKKFYPDVVNPNGFSTLTITLVNSTTTPITNVTLTDSLSTMGGTPPT
                     SGVYIADNPNPVVTCAGGVIDYPDGADNALGPNERTIRMTGGTIPPRVGTTDGACTIS
                     IDVQGRGAGATRTNTIPVANASGTISNTTVGPRAPATATLTIGSLSIDINKKFDPILV
                     YGGAASTLSVTISNPNSSPLTGITFTDFLPAGMIIATPADLNPGPLCGPNAKLTGVSG
                     SDSFTFSDGELPPSRSCVMSLRVTMNVNGNLTNTIPAGAVTTFNGVTNPNPIQATLSN
                     LAGASISKGFSPNPVLAGNDNYSTLTITITNTSGLDLTGMGLVDTLPGTPPTGLVIAG
                     APAPAPSTTCSLGTLTAVPGTQHIELTGATLLRNSSCFITVPVTSETPGNYANLISAG
                     TLISDQNITNPDPATAALLVISTTASPTTGTVGETITAGDSINLSGANNPTGSVTFTL
                     YSDAQCTIPVPGMSGSGVIANGAASWSNTWTPTAPGIYNWVASYPGDANNNAFTTKCG
                     DVNEQIEITAIHPSIEV"
     assembly_gap    3670..3769
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <3770..4960
                     /locus_tag="P5516_08320"
     CDS             <3770..4960
                     /locus_tag="P5516_08320"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRT92475.1"
                     /translation="SDAGRQIVFNFGTVANSDNVARIITIRYRVVLLDIASVVQGVNL
                     NNDVRWDWTGGSLTTNNPTLEVVEPHLALNKSANVAMAMPGSSITFRLEVSYVGDGSP
                     AYDVLLMDKVPNGITYEPGSFRFLSGQAPVLDDSSAPTLKATWATFQNTGVPTILEFK
                     GTLANLPSGTTVTNTAYLSWSSLPGDVTDPQSPYNDYSVERNYDPGSPVNVYGVSSSA
                     TITVMRKILPSSGFAPGRVTVLPSQPAQAAYNSLGNLWLEIPKLNVQIPILGIPAKEA
                     TWDLTWLSNQAGYLEGTAFPGAVGNSVLTAHVYKADGSSGPFVNLGNLRWGDQVILHN
                     QGQRYIYEVRSNQMILPGDTSILRHEKYSWLTLFTCKGYSETRGDYLYRVAVRAVLVR
                     VEAE"
BASE COUNT         1233 a         1461 c         1271 g         1063 t
ORIGIN      
        1 gcctttacat ggaatccagc gcatgcggat gatccataca cccagccggc gatcaacaag
       61 acattttctg atattgtcat tatcccaggg gtgaccaccc agctgacggt gaaagtattt
      121 aatgaaaacc aattcggact ggtggatatc gcctatacgg atgttatgcc gccgaatatt
      181 aagatcgtga ccccggcagt ggcgagcaac tcctgcgggg gtacggtcac cgctgttccc
      241 ggtacgaata cattttccat cagcgggggt tctgtccctc ctaaaacaac atcagtcccg
      301 gcggaatgtt ctctcgtctt gaacgtgacc tcgaccgtgg caggtaccca tatcaataca
      361 atccacgcgg gtgatctctc agcccgggac gcggatcggg cactgcccgt tttgcataat
      421 gagtaccctg ccagcgctac cttacaggtg ctcgttgttc aaccgccttc gttgagcaaa
      481 atcttcaacc cgaccacgat cttcgtgggc gaagtaagcc gcctgaccat caccatccgc
      541 aataatgatc ttctcaatga tctgcacgaa accaccttca ccgataccct gcctgcagga
      601 gttgtgctgg cgccaaccgt gaacccggtg ctgacaggtt gcgggaccgg cacggtgacc
      661 gccgtttcgg ggacgaacac cattacgctg aacaacgcta cagttaccaa gaacagcact
      721 tgttcagttg cggtcaatgt tacttcgagc acccaaagcg acactcccta tgtgaatacc
      781 attcctgcag ggccaggaag cggggcgatc agcacacgag agggcgtgac caacgcgacg
      841 gcggcaagcg cctcattata tgttcaaaat gtgggtatcg caaagtcatt ctcaccgact
      901 tctattgttg ccggcgcaac cagcacgctt accatcacct tgcgcaatcc aacgggcaca
      961 gcctacactg gggcgtctat ttcagacacc ctgccggcag ggctgatcat cgccccggca
     1021 ggagggtcta ccacctgcgg ggggggggac tatcgactac cccgatgggg ctgacaacac
     1081 gctgggtgcc aatgaaagaa ccattcgcct cactggcggc accatccccg ccagccctac
     1141 tccgccaaca ccttacggta cctgtaccat cacggtggcg gtcaccacct tgcccgatgc
     1201 cccggcagcg accttgacga acaccattcc accaaattct ctgaccactg accaggggat
     1261 caccaaccct tctgctgtaa cagccaacct agcgattacc cgctggctga caggtacaaa
     1321 ggctttcaat cctgcatcca taccaacagg cggtacgtcc actgtcacta tcaccctgag
     1381 gaacaatcgc acggatgtaa acggtgacct gacagatgtt acgtttacag acaccctgcc
     1441 tgccaacgtg actgtttcag gcgtgccggc taccccccag tgcggaggta cagtttcata
     1501 tactgctaca acggtaacat tggttggagg gtcgatccca aggaattcca gttgcaccat
     1561 cgtctttcag gtgaccactt ctgccaatgg cacgtacagc aacaccattc cagcagggga
     1621 tatcagtaca gggcagggcg cgagcaacgt tctcatcact tccaataacc taagttccat
     1681 aaccggcggc ggaccggtga gggtaagcaa gaattttcaa accagcccca ttgcaccggg
     1741 agccaatgtg agattaagga tcagactcac ctctcccacg gatattggta tttcagggat
     1801 taatttcact gatacccttc cagctggcat gcgcatcacg aacagcacac ctgccagcaa
     1861 cgcctgtggt gggactctca catcaatcac cggcacaaat cagatcaccc tgacaaatgg
     1921 agcgattgcg gctcctgata ctgcttgtaa tattgatgtt tatgtgaccg cagatgattc
     1981 catgtcatat acgaacacca tcccagtagg agccatcacg accgcggaat taaggtcgaa
     2041 caccagcgca gcttctgcga cactggtcgt ttctggcttc accatgagca aaaagttcta
     2101 tcctgatgtg gtcaacccta atggtttttc caccctgacg atcaccttgg tgaacagcac
     2161 cacaacaccg atcaccaatg taacactgac tgattcactc tcgaccatgg gaggaacgcc
     2221 accaaccagc ggagtctata tagccgacaa tcccaaccct gttgtcacct gcgccggcgg
     2281 ggtgatcgac taccccgatg gcgcagataa cgctctcggc cccaatgagc gtaccatccg
     2341 catgacaggt gggacaatcc ctccaagggt cgggacaact gatggcgcct gcacgatctc
     2401 catagatgtg cagggtagag gcgcgggggc aacacgtacg aataccattc cggtggcaaa
     2461 tgcgagcggg acgatctcta acaccacggt tggtccacgg gctccagcga ctgccaccct
     2521 gaccatcggt agcctttcca tcgatatcaa caagaaattt gatccgatcc tcgtctatgg
     2581 tggtgcggcg tctacattaa gcgtcacgat ctcaaacccg aatagttctc cccttaccgg
     2641 aatcactttc actgatttcc tgccagccgg catgatcatt gccactccag cggacttgaa
     2701 tccgggcccg ttgtgcggac caaacgcaaa actgaccggg gtttcaggtt cagactcatt
     2761 caccttcagc gatggcgaac tacctccaag caggagttgc gtgatgagct tgcgggtcac
     2821 catgaacgtt aatggcaacc tgaccaacac catcccagcc ggcgcggtaa ctacattcaa
     2881 cggagtaacc aatcccaacc ccatccaggc aaccttgtcc aacttggcgg gggcaagcat
     2941 cagcaaaggg ttctctccaa acccggtgct ggcaggcaat gataactatt caaccctgac
     3001 cattacgatc accaatacct cgggactgga cttgaccggc atgggactgg tggacaccct
     3061 ccctggaaca cctccgaccg gattggtgat cgcgggtgca cctgcgccag cgccatccac
     3121 tacctgcagc cttgggacgc tcactgcggt acccgggact caacacatcg agcttactgg
     3181 cgcaacgcta ttgagaaact catcttgttt catcaccgtc cccgtcacca gtgaaacgcc
     3241 cggaaattat gcaaacctga tctcagcagg cacgctcatc tctgaccaaa atattaccaa
     3301 tcctgaccct gccactgcag ctcttttagt gatctcaacg actgccagcc caaccacagg
     3361 cacagtgggg gagacgatca cagcggggga tagcatcaat ctgagcggag ccaacaaccc
     3421 gactggctcg gtaactttca cgctgtacag cgatgcgcag tgcaccatcc cggttccggg
     3481 gatgagcggc agcggggtaa tcgctaacgg agcagcctct tggtctaata cctggactcc
     3541 gacggcgcct ggaatttata actgggttgc cagttacccg ggtgacgcaa acaataatgc
     3601 tttcacgacc aaatgcgggg atgtgaacga acagatagag ataaccgcga tccacccatc
     3661 cattgaggtn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     3721 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnna gcgacgccgg
     3781 gcggcagatt gtcttcaatt ttgggacggt ggcgaacagc gataatgtag cgcggataat
     3841 cacaatccgc taccgtgtgg tgttgttgga tatcgccagc gtggtccaag gagtcaacct
     3901 taataacgac gtgcgctggg attggaccgg cggcagtcta accacaaaca acccgaccct
     3961 ggaggttgtc gagccgcatt tggcgcttaa taaaagcgca aatgtggcga tggcgatgcc
     4021 tggctcaagc atcaccttca ggctggaagt atcctacgtc ggcgatggat ctccggcata
     4081 cgatgtgctg ctgatggaca aagtgccgaa tgggattact tacgaacctg gctcatttag
     4141 attcttgagc ggacaggcac ctgttcttga tgatagtagc gcaccaacgt tgaaagccac
     4201 ttgggctacc ttccagaaca ccggggtacc cactattttg gagttcaagg gaacgctcgc
     4261 caaccttcca agcgggacaa cagtgaccaa cacggcttat ctgagctggt ccagcctgcc
     4321 tggagatgta accgacccgc aatctccgta caatgattat tctgtggaac gcaattacga
     4381 cccgggcagc ccggtgaatg tgtacggtgt cagcagcagt gcaaccatca ccgtcatgcg
     4441 taagatcttg ccttcttccg gtttcgcgcc aggacgggta acggtgctcc cttcgcaacc
     4501 cgcacaggcg gcctacaaca gcctggggaa tttatggctg gaaataccca agcttaatgt
     4561 tcagattccc atcttaggta ttccagcaaa ggaagccacc tgggacttga cctggttatc
     4621 gaatcaggcg ggttacctgg aaggcaccgc tttcccgggt gcagttggta attcagtgct
     4681 gaccgctcac gtatataaag cggatggcag ttcgggtccg tttgttaacc tcggcaacct
     4741 gcgctggggt gaccaggtca tcctgcacaa tcagggtcag cggtatattt atgaggtgcg
     4801 ctcgaaccaa atgatactcc ctggagatac ttccatcctg aggcatgaaa aatactcctg
     4861 gctgaccttg ttcacctgca agggttacag tgagacgcgg ggagactacc tgtatcgtgt
     4921 tgctgttcgg gcagtgctgg tgagggtgga agccgaataa gacactgcaa aaaaagaatc
     4981 aatttgaaca actgccggca ttattcgtgc cgacagttgt ttttttgggg gagggactgg
     5041 ttgatgcagc attcattctg aaaaatgaaa atccctttgc agacgaaata gcagaaaaag
     5101 ggcttgaagc aatcccggag ctgatgcg
//
LOCUS       DAOTLW010000073         5024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_14692_length_5024_cov_11.009459, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000073 DAOTLW010000000
VERSION     DAOTLW010000073.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 5024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5024
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_14692_length_5024_cov_11.009459"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(97..169)
                     /locus_tag="P5516_08325"
     tRNA            complement(97..169)
                     /locus_tag="P5516_08325"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(134..136),aa:Thr,seq:cgt)
     gene            complement(274..1473)
                     /gene="dinB"
                     /locus_tag="P5516_08330"
     CDS             complement(274..1473)
                     /gene="dinB"
                     /locus_tag="P5516_08330"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HRT92476.1"
                     /translation="MSKSATRKILHVDLDAFFCAVEEQREPSLAGKAFAVGGKADQRG
                     VICSCSYPARRKGVRSAMPTGQALRLCPELILISTHFGEYSAKSSQVMEIFHQLSGLV
                     EQVSVDEAFIDVTDLPEPVEQIARALQARVKLDTSLPCSIGAASNKLVAKIATDTGKN
                     QSRTDGYPQAILCVPAGTERSFLSPLPVRAMWGVGPKMESILLGMGLRTIGDIANCSV
                     DRLERRFGKSGVDLHQHALGIDNRPVTVSYEAKSISQEVTFARDTTDLLFLRRTLHEL
                     AIKVGYRMRCEGLCARVIRLKLRFADFTTHTRQVMLQAPTDQDSVIATEAETLLMKLW
                     QPDRPVRLIGVGGANLVEHVHQLTLWESKTQKERKLLIALDELREKYGKEAVQRADHI
                     RKRHKEE"
     gene            complement(1563..2618)
                     /locus_tag="P5516_08335"
     CDS             complement(1563..2618)
                     /locus_tag="P5516_08335"
                     /inference="COORDINATES: protein motif:HMM:NF021916.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphoglucose/phosphomannose
                     isomerase"
                     /protein_id="HRT92477.1"
                     /translation="MDLNEHQHFAQIDREDMLGHIMGLPLQLEKAWHLGKKQPLTGIQ
                     PVERIILAGMGGSAIGGDLLASYAADQLEVPLIVHRDYDLPACASGAGTLVVASSHSG
                     NTEEVLSAFERALRNKCQLIAMCTGGKLEALAREHHVPLWKFEHHGQPRAAVGYSFGL
                     LLALLTRLGLLPDPEREAQAAIKGMRALQEKIDAPIPVLKNPAKRLAGQLIGRHITVF
                     SSGFMSPVARRWKTQLNEVAKAFASFEYLPEADHNTLTGIWNPPGSETREFCLFLLAG
                     EDHPRNLLRLEKTKRILMLEGIGTDLFTAVGETRLEQMWNALLFGDYLAFYLAMIYDT
                     DPTPIPPIATLKEEMSA"
     gene            complement(2697..2876)
                     /locus_tag="P5516_08340"
     CDS             complement(2697..2876)
                     /locus_tag="P5516_08340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92478.1"
                     /translation="MSENFDPKVTTIAETDSYAVWSAEEPDGETTYNLELNNVTIHMF
                     EEEYAEFLELVKKLK"
     gene            complement(2869..4143)
                     /locus_tag="P5516_08345"
     CDS             complement(2869..4143)
                     /locus_tag="P5516_08345"
                     /inference="COORDINATES: protein motif:HMM:NF013740.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HRT92479.1"
                     /translation="MMSLQENPSSPGWSTNTKLVVGLALAGITILLLIRFHNILAPLL
                     TSVLVAYLFHPIATFFNKKLKIPWRLITTILFLLLFAIIIGLLTWGGISIVEQVQNLI
                     KLIQGMITDLPGFFEEISSKPLVIGPFEFDLSKLELETLWTQVAGIVQPLLAQLGSLI
                     GSLATGIGSTLTWLVFILLIAYFILSESNKANLIRIVIPRYQEDIQKIVTQLGHIWNA
                     FLRGQLLIFVITVAYYALLLSVLGVKNLFLLAFLAGLARFVPYVGPFVAWTTYGLVAL
                     FQTEHLGMQPLPYALLVVGCAWFSDFLLDNFVVPRVMSDALAIHPAAVLVMVIISASL
                     FGFLGILLAAPVLASIQLIVTYLISKLADQDPWLNLRVIQPPEKLSVIFIRQWQRTKN
                     AFLRINIFKRDTAKKQANNPQQSSKKKGEKNE"
     gene            complement(4217..4951)
                     /locus_tag="P5516_08350"
     CDS             complement(4217..4951)
                     /locus_tag="P5516_08350"
                     /inference="COORDINATES: protein motif:HMM:NF028007.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HRT92480.1"
                     /translation="MANFRLILQSGSAAGTEYPLEKTEIYLGRDLSNDVVINDPEVSR
                     RHARLVLTGNTYVIEDLGSTNGTFLRGQRLSAPMLLNPGETITIGENVLLKFEGASID
                     PNATVAAFRRSSAEPVTSAAPAQQPFVPQQPAAPRYTPAQPAAPQYGAPQPAAPQFVP
                     QQPVTPPFATPQPAPPVHAAVGGASPAPYAPTAKKKSGWLIALLVVVGVLLIFCIVPW
                     LIIEVTDSYCALFPGIFNSIQPGVCP"
BASE COUNT         1252 a         1299 c         1252 g         1221 t
ORIGIN      
        1 ttgagccacc gaagggattt gcgagcgcct tgtgcgagtg ctggatgttc ctgccagtaa
       61 acccttgacc tgagcttttt ttgccggaaa tgattgagcc accgaaggga ttcgaaccct
      121 tgacctgacg tttacgaaac gcctgctctg ccagctgagc tacggtggct tgcttcgaac
      181 ctcgaaatta taatgcactc gtcacccaga ggcaagcaaa cagagcagaa taaagacaga
      241 atgaagaaaa cagagaggta catagttccc ggttcactcc tctttgtgcc gcttgcggat
      301 atggtcagcg cgctgaacgg cctcctttcc atatttctcg cgtagttcat ccaacgcgat
      361 taacaatttc ctttctttct gcgttttgct ttcccacagg gtaagctggt gaacatgttc
      421 caccaggttc gcccctccaa caccaatcaa acgcaccggg cggtcaggct gccataattt
      481 cattaataac gtttcagctt cggtggcgat cacactatcc tggtcagtgg gcgcctgcag
      541 catcacctgc cgggtgtggg tggtaaaatc agcaaaacgc agtttgaggc gaatcactcg
      601 cgcgcataaa ccctcacaac gcatacgata gcccaccttg attgcaagtt cgtgcagggt
      661 gcgccgcaag aaaagcaagt cggtggtatc gcgggcaaat gtaacctcct ggctgatgga
      721 tttggcttca taagatacgg tcactggtcg attgtcaatc cccagggcgt gctgatgcaa
      781 atcaacaccg ctttttccaa atcgcctttc caaccggtca acagagcagt tggcgatatc
      841 accaatcgtg cgcaacccca tccctagcag gatggactcc atcttcggtc caacacccca
      901 catggctctc accggtaaag gagacaaaaa gctcctttct gtacccgcag gtacacataa
      961 gatcgcctgc gggtacccat ctgttctact ttggtttttg ccggtgtctg tggcaatctt
     1021 tgccacaagt ttattactcg cggcgccaat tgagcacggt agactggtgt ccaatttcac
     1081 tcgggcctgc aatgcgcgcg cgatctgctc taccggttct ggcaaatcag ttacgtcaat
     1141 gaacgcttca tccaccgata cctgttccac cagcccgctc aactgatgaa agatttccat
     1201 cacctgacta gattttgcgg agtattcgcc gaaatgggta gagatcaaaa tcaattcagg
     1261 gcataagcgc agcgcctgcc ctgtaggcat ggctgaacga accccttttc tgcgagccgg
     1321 gtatgaacag gagcaaataa ccccgcgctg gtcagccttt cctcctaccg caaaagcctt
     1381 acccgcaagc gaaggttccc tttgctcttc aaccgcgcaa aaaaacgcgt ccaggtcaac
     1441 atggaggatt tttcgtgttg cgcttttact catgctgaat ttagtatagc aaatctgttc
     1501 taattgtcaa gaaacaaact gcaggcaacc gcgcctgcag tccgctggtg ttaatgatgg
     1561 gtttatgcgc tcatttcttc tttgagtgtg gcgataggag gaatgggtgt agggtccgtg
     1621 tcgtaaatca tcgccaggta aaacgccagg taatctccaa agagcagcgc gttccacatc
     1681 tgctcgaggc gtgtttctcc tacggcggta aagagatccg tgccaatccc ttccagcatg
     1741 aggatgcgtt tggtcttctc caaacgcagt aagttgcgcg gatgatcctc ccctgccagc
     1801 agaaacaggc aaaattctct cgtctcgctg ccgggtggat tccaaatacc ggtcagggta
     1861 ttatggtcag cctcaggtaa atactcaaaa gacgcgaacg cctttgccac ttcgttgagc
     1921 tgcgttttcc agcgccgcgc cacaggagac ataaagccgc tggaaaacac agtaatgtga
     1981 cgcccaatca attgccccgc cagccttttt gctggatttt tcagcaccgg tattggcgcg
     2041 tcaattttct cttgaagcgc tctcatccct ttaatggccg cctgcgcttc ccgctcaggg
     2101 tctggcagca aaccaagcct ggttaataac gccaataaca gcccgaatga ataccccacc
     2161 gcggccctgg gttgaccatg atgttcaaat ttccataacg gaacatggtg ctcccgcgcc
     2221 agcgcttcca gctttccgcc agtacacatc gctatgagct ggcatttgtt tcttaaggct
     2281 cgctcgaaag ccgacagcac ctcttccgta ttaccggaat gagaagaagc caccaccagc
     2341 gttcccgcgc ctgaagcgca cgccggcagg tcataatcgc ggtgaacgat taaaggcacc
     2401 tctaactgat cagcggcgta agaagccagc aggtcgccgc caatggctga gccgcccatc
     2461 ccggccagaa tgatgcgttc cactggttgg attccggtta atggctgttt ttttccgaga
     2521 tgccaggctt tctccagttg tagtggcaaa cccataatat gcccaagcat atcctcgcga
     2581 tcaatttggg caaaatgctg atgttcgttt aaatccatgc acaatccttg attgtcgttt
     2641 ttattggagc gtccaatcaa tcatcacaac agagcagatg gtcgctctgt tattccttat
     2701 ttaagttttt tcaccaattc cagaaactcg gcgtactctt cttcaaacat gtgaatcgta
     2761 acgttgttca attccaggtt ataagtcgtc tcgccatccg gttcttccgc agaccaaact
     2821 gcgtaactat ctgtttctgc aatcgtggtc actttcggat caaaattttc actcattttt
     2881 ttctcctttt ttcttacttg attgttgtgg gttatttgcc tgttttttag cagtgtctct
     2941 tttaaagata ttgatcctca agaaagcatt tttcgtgcgc tgccactgcc gtatgaagat
     3001 taccgatagt ttttccgggg gttggatgac cctcaggttc agccagggat cctggtctgc
     3061 gagtttactt atcaagtagg tcacgatgag ttgaattgaa gccaaaacag gagccgctaa
     3121 cagaattccc aggaacccga acaggctggc tgaaatgatc accattacga gtacagcggc
     3181 aggatgaatg gcgagcgcat ctgacattac tcttggcact acaaaattgt ccagcagaaa
     3241 atcagaaaac caggcgcagc ccaccaccag cagcgcgtat gggagtggct gcattcccag
     3301 gtgctcggtt tgaaatagcg ccaccaagcc atatgtcgtc caggctacaa acggcccgac
     3361 gtaagggaca aagcgcgcga gccccgctaa aaacgcgagc aggaataagt tcttgacccc
     3421 cagcacgctg agcagaaggg cgtaataggc aacggtgatg acaaaaatga gtagctgccc
     3481 acgcaggaag gcgttccaga tatgccctag ctgggtgacg atcttctgta tatcttcctg
     3541 gtacctgggg atgacaattc tgatcaaatt cgccttgttg ctctccgaca gaataaaata
     3601 cgcaatcaga agaataaaga ccagccaggt gagcgttgaa ccaataccag ttgccagcga
     3661 accaataagg ctacccagtt gtgccaataa cggttggacg atccccgcaa cctgagtcca
     3721 gagtgtttcc aattcgagct tcgaaaggtc aaattcaaaa ggtccaatga ccaaaggttt
     3781 actggaaatt tcctcgaaaa atccaggcag gtcggtgatc attccctgga ttagtttgat
     3841 caggttttgt acctgttcaa caatggaaat accgccccag gttaacagcc cgataatgat
     3901 ggcgaacaag agcagaaaaa ggatggttgt aatcaggcgc cagggaattt tcagcttctt
     3961 attaaaaaaa gtcgcgattg gatggaataa atacgctacc aacacggagg tgagcaacgg
     4021 cgccaggata ttgtgaaaac ggatgagcaa gagaatggta atgccagcca acgccagtcc
     4081 caccacaagt tttgtgttgg tcgaccagcc tggcgaagag gggttttcct gaagtgacat
     4141 catgattatt ataagggata gtggttaaaa aacgaaactg gctttacgca gccagtttcg
     4201 tttggttttt ttgggtttac gggcagacgc ccggctggat tgaattaaag atccccggaa
     4261 aaagcgcgca gtaactatct gtgacctcaa tgattagcca ggggacaatg caaaagataa
     4321 gcaaaacacc caccacaacc agtaatgcga tcagccatcc agatttcttt ttcgccgtcg
     4381 gcgcgtacgg agcgggggaa gcccctccca cagcagcgtg gacgggaggc gccggttgtg
     4441 gcgtggcgaa gggaggggtc acaggctgtt gcggcacgaa ctgcggagcg gcgggttgag
     4501 gtgcgccgta ttggggcgca gcgggctggg cgggcgtgta acgcggcgct gcaggctgct
     4561 gaggtacgaa aggttgttgg gcaggtgccg cggaggtaac tggctcggca gaagagcgcc
     4621 tgaaagccgc cactgtggcg ttaggatcga tggacgcgcc ttcaaatttg agcagaacat
     4681 tttctcctat ggtgatggtt tcacccggat tgagcaacat gggcgcgctt aatctttgcc
     4741 cgcgcaaaaa agtgccgttg gtagatccca ggtcctcaat gacatacgtg ttcccagtca
     4801 gtaccaaccg ggcgtgtcgg cgagaaactt cagggtcatt gatgaccaca tcattgctca
     4861 agtctcggcc aaggtagatc tcggtctttt caagcgggta ttccgtcccg gcagcacttc
     4921 cactttgaag gattaaacga aaatttgcca tatgctcctc cttatcattt tattagtgta
     4981 gcaagtgaat gtgagaatgt caacgatttt aataattttg attt
//
LOCUS       DAOTLW010000074         4772 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_15580_length_4772_cov_9.672673, whole genome shotgun sequence.
ACCESSION   DAOTLW010000074 DAOTLW010000000
VERSION     DAOTLW010000074.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15580_length_4772_cov_9.672673"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            68..292
                     /gene="secG"
                     /locus_tag="P5516_08355"
     CDS             68..292
                     /gene="secG"
                     /locus_tag="P5516_08355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HRT92481.1"
                     /translation="MELYIDIALIITSIALIASVILQNKGVGLGGLTGQDSGGVFTAR
                     RGIEKTLFWVTIVLSGLFFILTFAAVLIQG"
     gene            379..2079
                     /locus_tag="P5516_08360"
     CDS             379..2079
                     /locus_tag="P5516_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012706.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HRT92482.1"
                     /translation="MKKYRWQLLVLFLTGLVVGTLLLLEKRGGLGEVNTPQPAQGGVY
                     TEALIGYPQRMNPLLDSTNSVDRDVDKLIFSGLVKFDSRGIPAPDLAETIGISQDGIL
                     YNISLKPGLVWHDGEKLTTKDVLFTIELLRTGGVYIPEDIRKLWDSIEVYLFDDQHMQ
                     LKLPEAYAPFMDYLTFGVLPQHLFAGMDINEIAASAINLQPVGSGPYRFGELLMEGST
                     VSGIRLLANEDYAGGRPYLQEIVFRYYPDASSAYQAYVQGAVQGISEVPTDLLPQALA
                     NEDLRIYTGRLPRISLVLLNLNDPGVPFFKEIEVRKALMLGLNRQAIISQMFNGQAIV
                     ANSVILPGTWAYNESIAEIGYDPQLAAETLKNAGYVIAGENNTTRAKEEVPLSFVLSY
                     PDDDLHLGIAQLIANNWQALGVNVTLEGVPPDVFVAEKLEPRSYQAALVDLNLSRTPD
                     PDPYPFWDLGQAENGQNYSQWNNRLASDTIEQARVTTDLGERTRLYHNFQAIFAEELP
                     ALPLYYPVYNYAVSEQILGVTMGPLFDTSDRLGTITTWYLTTSRNQKTTDTAETDTGN
                     "
     gene            2273..2617
                     /locus_tag="P5516_08365"
     CDS             2273..2617
                     /locus_tag="P5516_08365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92483.1"
                     /translation="MNKVEKLIDTRRQAWENRARQEIPLRLNQYRELVDEYLEQGAID
                     ASYRSNISTRVKLDLLFRSLAAEPYVEQRKAQELDRQLGALLPEGDFIWEPELETGFP
                     KSIYAYLYVRKK"
     gene            2624..3181
                     /locus_tag="P5516_08370"
     CDS             2624..3181
                     /locus_tag="P5516_08370"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:TIGR00636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HRT92484.1"
                     /translation="MKKFYTGTGDKGSTGLLGEQRVDKFDLRMEALGTLDELSAVLGI
                     ARSMLTDPQDRQKIILYQRKLYQLMAEVAASPENREKFAKINEQSVNSLEAEIARISM
                     RVDTPAGFILPGETPTSAYIALARTITRRAERRVAELTSRGDLTNAYVLIYLNRLSSL
                     FFAMELDLIQRETSHKPTMARESST"
     gene            3178..3882
                     /locus_tag="P5516_08375"
     CDS             3178..3882
                     /locus_tag="P5516_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HRT92485.1"
                     /translation="MTGTLINYAGILVGGLTGLLLGSRLKEKLKQTIISALGLFTLLY
                     AISLFLETQNALIVLGSLLIGILLGEWWRIEEGLMALGEKLEGRLNHSGRGEGSSRFI
                     QGFLSSTLLFCIGPMAVLGSLQDGLSGGSYTLIIKSILDAFAALAFASSLGAGVLFSS
                     VPVLLYQGSITLLAQQVQRVATPEMLNELSATGGVILAGLAISNIMEIKKIRTGNFLP
                     ALLVAPLLVFFLSLFK"
     gene            complement(3929..4624)
                     /locus_tag="P5516_08380"
     CDS             complement(3929..4624)
                     /locus_tag="P5516_08380"
                     /inference="COORDINATES: protein motif:HMM:NF024853.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HRT92486.1"
                     /translation="MTKRDVVQQRRKQNRSKNSLTTILIIAAFVFVVVGMVILTQYKP
                     VGQIATITRTTTAQKDGLSLGNPDAPVQVIEFADFQCPACANYWSVLEPQIIKDYVET
                     GKARFTYSPFSFLGQGQAWDESIKAAEAAYCANDQQKFWEYRDMVFANHNGENRGAYS
                     KERLVAFAKQLDLDMKAFNACFNSGKYSQQVINDNKFATEQGATYTPSFLINGKIVNA
                     AELVQAIETSLTN"
BASE COUNT         1196 a         1111 c         1232 g         1233 t
ORIGIN      
        1 attaggttga cgattttcgg ttatgaacgg tataatctgc ggtgtttgaa atttcaggag
       61 gtttctggtg gaactttaca ttgacatcgc gctaattata acttcgatag ctcttatcgc
      121 gagcgtaatc ctgcaaaata aaggggtagg gcttggagga ttgaccggtc aggattctgg
      181 cggtgtcttc actgcccgca ggggaatcga aaaaacgctg ttttgggtga cgatcgtact
      241 cagtggttta ttttttattc ttacctttgc cgcggtcctt atccaggggt agaccgggtc
      301 attcaaggaa cagcctgaaa ccaatcattc gaggcgacta accttgaaac agggtagtcg
      361 ccattttcta taaaccttat gaaaaaatat cgctggcaat tactcgttct ctttctgact
      421 ggtttggtag ttggaactct cttgctttta gaaaagcgtg gcgggctggg agaagtgaac
      481 actccacaac cggcgcaagg aggggtttac actgaggccc tgattggata cccccagcgc
      541 atgaacccgc tgctggatag taccaatagt gtggatagag atgtagataa actgattttc
      601 tctggcctgg tgaaatttga ctcaagaggt attcccgctc ctgacctggc cgaaacgatc
      661 gggatttctc aggatgggat actgtataac atctcgctaa aaccagggct ggtttggcat
      721 gacggcgaga agctgaccac caaagatgtt ttgttcacca tcgaattgct cagaacagga
      781 ggtgtctata ttccggaaga tatacgcaaa ttatgggatt cgatagaagt gtatctcttc
      841 gatgaccagc atatgcaatt aaaacttccg gaagcctacg cgccttttat ggactatctg
      901 acctttggcg tgttgccaca gcaccttttt gctggcatgg atatcaacga aattgccgca
      961 tccgccatca accttcaacc ggtaggcagc ggcccgtacc gctttggtga actgctgatg
     1021 gaggggtcaa cagtgagcgg cattcgactt ctggcaaatg aagactatgc cggtggacga
     1081 ccctatttgc aagagatcgt tttccgttat tatcctgacg catcaagcgc ctatcaggca
     1141 tacgttcagg gtgccgtgca gggcatcagt gaagttccca cagacctgtt gccgcaagcg
     1201 cttgcgaacg aggatcttcg tatttataca ggacgcttac cgcgcatcag cttggtgtta
     1261 ttgaatctaa atgacccagg cgtcccgttt ttcaaggaga tagaggtgcg caaagcgttg
     1321 atgctgggtt tgaatcgcca ggcgatcatc tcgcagatgt tcaacgggca ggcgattgtg
     1381 gcgaatagcg ttatcctccc tggaacttgg gcttacaacg agagtattgc tgaaatcggt
     1441 tatgacccgc aattggcagc cgaaaccctc aagaacgcgg gttacgtgat cgctggtgaa
     1501 aacaatacca ccagggcaaa agaagaggtt ccgctatctt ttgtgctgtc ttatccagat
     1561 gatgacctgc accttggaat agcacagtta atcgccaaca actggcaagc cttgggggtg
     1621 aacgttacac tggagggcgt acccccagat gtgttcgttg ctgaaaaact agaaccgagg
     1681 tcttaccagg cagctctggt cgacttgaac ttaagccgca cgcctgaccc tgacccctac
     1741 ccgttttggg acttggggca ggcggaaaat gggcagaatt actcccagtg gaacaatcgc
     1801 ctggcaagcg atacgatcga gcaggcgcgc gtgaccactg acctgggtga gcgaacccgt
     1861 ctctaccata atttccaggc gatcttcgcg gaagaattac cggctctacc gctctattac
     1921 ccggtataca actacgcggt ttcagagcag atactgggcg tgacgatggg tccgctcttt
     1981 gataccagcg accggctggg aacgatcacc acctggtatc tcactaccag ccgcaaccaa
     2041 aaaaccacgg atacagcaga aacagataca gggaactgaa atgtcgtacc agggcatgga
     2101 ggacgacata cggctaatcc ttgctgctgg agaagaactg gcgcaatatc tgtcttcccc
     2161 catcattctc tggagggtga aacactgcaa ccaacctctt tcgcctggta acctgcttct
     2221 ggctattacc cggctaaaaa cagttaatga aattgcggtg cagcatgatg aaatgaataa
     2281 ggttgaaaaa ctgattgaca cccgacggca ggcgtgggaa aatcgtgcgc gtcaggagat
     2341 ccctttgcgc ttgaaccaat atcgcgaact ggtcgatgaa taccttgagc aaggagcaat
     2401 cgacgcaagt taccgcagca atatcagcac ccgggtaaag ctagatttgc tttttcgaag
     2461 cctggcggca gaaccatatg tagagcaacg taaagcgcaa gaactggatc gtcaacttgg
     2521 cgcgttgttg cctgaaggtg attttatttg ggaaccagag ctggaaacgg gtttccccaa
     2581 atcaatctat gcctatcttt atgtcaggaa aaagtgaact atcatgaaaa aattttatac
     2641 aggaacaggt gataagggct caaccggttt gcttggcgaa cagagagtgg acaaatttga
     2701 tttgcgtatg gaggcgctgg ggacgctgga tgagctatca gcggtgctgg gaattgcccg
     2761 cagcatgctc accgacccgc aagaccgcca gaagatcatt ctgtaccaac gcaagttata
     2821 ccaattaatg gccgaagtgg cagcttcgcc agagaacagg gaaaaatttg ccaaaattaa
     2881 tgagcagtcg gtgaactctc ttgaagctga aattgcccgt atttcgatga gagtggatac
     2941 acccgcagga ttcatattgc ccggagaaac accaaccagc gcgtatatcg ccctggcgcg
     3001 taccattacc agaagagcag aacggcgtgt tgcagaactt accagccgtg gagacctgac
     3061 aaacgcgtat gtcttgattt accttaatcg tctttcgtcg ctcttttttg caatggaact
     3121 ggacctgata cagcgtgaga cctctcacaa accaaccatg gccagggaat cgtcaacttg
     3181 actggaacat taatcaacta tgctggcatt ctcgtgggag gattgacagg ccttttactt
     3241 ggatccaggc tgaaagagaa attgaagcag accatcatca gcgcgctggg tctcttcaca
     3301 ctcctgtacg cgatatcgct ttttcttgaa acgcaaaacg cgctcattgt gctgggaagc
     3361 ctgcttatcg ggattttgct gggagaatgg tggcgaatcg aagaggggtt gatggcgctg
     3421 ggggagaagc tggaaggtcg tttaaaccat tccgggcggg gagaaggctc cagccggttt
     3481 atacagggct ttctttcgtc aacactgttg ttctgtatcg ggccaatggc ggttttgggc
     3541 tctctacagg atggattgag cggaggttct tacacgctca ttatcaagtc catcctggac
     3601 gcttttgccg cgttagcctt tgcttccagt ttgggcgcgg gagtgctttt ttcttctgtc
     3661 cctgtattgc tttaccaggg ttctatcacc ctgttggcgc aacaggtaca gcgtgtggcg
     3721 acccctgaaa tgctgaatga gctcagcgct actggtgggg tgatcctggc gggactggcg
     3781 atcagcaata ttatggaaat aaagaaaatc cgcacaggga atttccttcc cgctcttctg
     3841 gttgccccac tactcgtatt ttttctatcc ctgttcaaat aaaccccggt ttacaaccgg
     3901 ggtttgtcat tctggtcatt tgcttcggct aatttgtcag actggtttcg attgcttgaa
     3961 ccaattcagc tgcgttgact atcttgccat taatgaggaa ggacggggtg taagttgcgc
     4021 cctgttctgt ggcgaactta ttatcgttta taacctgctg actgtatttt cctgaattaa
     4081 aacaggcatt gaatgctttc atatccaaat caagttgctt ggcgaatgcc accagccgtt
     4141 cttttgagta ggctccgcgg ttttcaccat tatgattggc gaagaccata tcgcggtatt
     4201 cccaaaactt ctgctggtcg ttggcgcagt aggctgcttc agcggcctta attgattcat
     4261 cccatgcttg cccctgacca aggaaggaga agggggaata ggtgaaacgc gctttgcctg
     4321 tttcgacgta atcttttatg atttggggct ctaaaacgga ccaatagttg gcacacgcgg
     4381 gacattgaaa atcagcgaat tcaatcacct gaactggcgc atccgggttg cccaatgata
     4441 gaccgtcttt ttgagcggtg gtggttcgcg tgatcgtggc tatttggccg actggtttgt
     4501 actgggttaa aatcaccatg ccaacgacca caaaaacaaa ggcggcaata attaaaatag
     4561 ttgtgagaga atttttggag cggttttgtt ttcttcgttg ttgcacaaca tctcgttttg
     4621 tcatctttcc tccataaaag tcctcgcatt atctcatata agactgaaat tgtccataaa
     4681 tatcccggtc aacaattgct ttgttcagcc ggatttcgtg aaaaccggga aggattcaga
     4741 ggtctggaaa aagcccagct ttttatagag ag
//
LOCUS       DAOTLW010000075         4737 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_15705_length_4737_cov_10.163392, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000075 DAOTLW010000000
VERSION     DAOTLW010000075.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4737)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4737
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_15705_length_4737_cov_10.163392"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /gene="selA"
                     /locus_tag="P5516_08385"
     CDS             <1..844
                     /gene="selA"
                     /locus_tag="P5516_08385"
                     /EC_number="2.9.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004125 - L-seryl-tRNASec selenium
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016260 - selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L-seryl-tRNA(Sec) selenium transferase"
                     /protein_id="HRT92487.1"
                     /translation="EIGGGFRIPEVMKQSGARLVEIGTTNRVNLEDYESALRDGAALL
                     MRAHTSNFKLVGFTSQPELKELTALAHQYEVPMLDDLGSGALLDTSRFGLAHEPMVQE
                     SLAAGVDLVCFSGDKLLGGPQAGIILGKAQLVAKIKRHPLARAVRADKLCLAGLEATL
                     LHYLKGEAEKEIPIWRMISASANDLKERATRWQQQVGGEVVEEHSTVGGGSLPEETLP
                     TFALSFNIPGLEQMAARLRQSTPPLIARVQGGRLYLDPRTVLPEEDEDLVRILRTHIT
                     GAHR"
     gene            841..2076
                     /locus_tag="P5516_08390"
     CDS             841..2076
                     /locus_tag="P5516_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012766.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HRT92488.1"
                     /translation="MKTDLDKLMKKHNVDAIFIIGAAQHNPSMVYFVGTAHVSDAILI
                     KQRGKDAVLFHYPMEREEAAKSGLKLVSMTKYPLHDLLKQAGGNYLEAFVLRLKLMLE
                     EMKLTRGRVALYGKDDIGPFYSILNRLQELMPELQFAGFVRDEILMNAMMTKDAGEIE
                     HIRAMGRVTTDVVAKTADYITGHKTKGGVVVHKDGTPVTIGEVKNLIQLWLAERGAEN
                     PEGTIFAIGRDAGIPHSVGNPSDALQLGQTIVYDIYPCEAGGGYYYDFTRTWSLGYVT
                     DEAQQLYDQVRQVYDTISAELKVNAPFYKFQERACALFEEMGHPTIQSDPTVERGYVH
                     SLGHGIGLRVHEKPWASAVNPSPTDILAPGSVFTLEPGLYYPERGMGVRIEDSWWVTP
                     DGEFEILAEFPKDLLLPMK"
     gene            2103..3131
                     /gene="tsaD"
                     /locus_tag="P5516_08395"
     CDS             2103..3131
                     /gene="tsaD"
                     /locus_tag="P5516_08395"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HRT92489.1"
                     /translation="MKLKSARLLGIETSCDETAAAVVENGREILSNVVATQIDLHTKY
                     GGVFPEVASRQHIRTIYPVIDEALQKANLGLADIDAIAVTRGPGLAGSLVVGMNAAKG
                     LALGRQLPLIGVNHLEGHIYSAWLYPADTPKPPPPPEFPQLALLVSGGHSELNLVQDH
                     LQYQRLGGTLDDAAGEAFDKVARMLRLPYPGGPAIQQAAREGNPQAFPFPRSRLENTW
                     NFSFSGLKTAVLREVRALEEENRSLPVPNLAASFQEAVVSVLFEKTMKAARDYAVKSI
                     LVAGGVSANEALRKTFTSQTEFPVHIPPLSLCTDNAAMIASAGYYHFCLGHVDPLDLD
                     VLPNWPLS"
     gene            3160..3768
                     /locus_tag="P5516_08400"
     CDS             3160..3768
                     /locus_tag="P5516_08400"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HRT92490.1"
                     /translation="MSAARQFPSEFVDRLIAGDGQAFNELVDQTSAGIYGLGLRMLGN
                     AQDAEDMLQETFLKAHNALRGFEKRSSLYTWLYRIATNEALMMLRRRKSEFHFDSEPV
                     TQEEAENGEEVVDWCCLPEKEFISEETRSELTRAAVKLSNALRMVFLLRDVRGFSTRE
                     TAEILQVNEEVVKTRLVRARVKLRDELSGYFRERLQGQGNNG"
     gene            3890..4024
                     /locus_tag="P5516_08405"
     CDS             3890..4024
                     /locus_tag="P5516_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92491.1"
                     /translation="MNTLQKTIDLYQVAEGEETLPEGVRSRLFARLQIAQSTGKKPGE
                     "
     gene            4017..4235
                     /locus_tag="P5516_08410"
     CDS             4017..4235
                     /locus_tag="P5516_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92492.1"
                     /translation="MSETSGMNQQPGKCPYSINAPAAVPSLKGERCLTGKPGQPCPVC
                     GKGVLDYDGWLVLGCAVCGFKEPGGSFT"
     gene            4330..>4737
                     /locus_tag="P5516_08415"
     CDS             4330..>4737
                     /locus_tag="P5516_08415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HRT92493.1"
                     /translation="MDAKLRWKYGLSFKGVADSNFEVPLGASVDHGGAGDGVVPMELI
                     LMGLGGCTAMDVISILEKKRQQVTDFEVKLHADRAGEHPKVFTDITIEYIIKGRNIDP
                     DAVARAVDLSENKYCPGMAMLRKAANIQTKYTIE"
BASE COUNT         1157 a         1124 c         1412 g         1044 t
ORIGIN      
        1 tgaaattggt ggaggattcc gtattccgga agtgatgaag cagtcaggcg cccgcctggt
       61 cgaaattggc accaccaacc gggttaacct ggaggactat gaaagcgcgt tgcgtgacgg
      121 cgcagcgttg ttgatgcgcg cgcacacttc aaattttaaa ctggtggggt ttacctcaca
      181 gcctgaactt aaagaactga ctgcccttgc ccaccagtac gaggtgccca tgctggatga
      241 cctgggttca ggggcactgc tggatacatc caggttcggg ttggcgcatg aaccgatggt
      301 gcaggagtca ctggcagccg gggtagacct ggtgtgtttt tcgggggaca agctgttagg
      361 aggaccgcag gcgggcatca ttttaggcaa agcgcagctg gttgccaaaa ttaaaagaca
      421 tccgttggcg cgtgccgtcc gggcggataa attgtgcctg gcagggttgg aagccacctt
      481 gctgcattat ctgaaaggcg aagcggagaa ggagatcccc atctggcgca tgatttcagc
      541 cagcgcgaat gacttgaagg aacgggcgac acgctggcag cagcaagtgg gtggggaggt
      601 cgttgaagaa cactccaccg tggggggagg aagcctgcca gaggaaacct tgcccacctt
      661 cgcgctatct tttaacatac cagggttgga acaaatggct gcgcggttac gccagtctac
      721 cccgccgttg atcgccaggg tgcagggcgg caggctttac cttgacccgc gtaccgtttt
      781 gccggaagaa gatgaggatc tggtcaggat actcagaacc catatcacag gagcgcacag
      841 atgaaaacag atctcgataa attgatgaaa aagcacaatg ttgacgcaat cttcattatt
      901 ggtgcagcgc agcacaaccc atcgatggtc tatttcgtgg gaaccgcgca tgtttctgac
      961 gcgatattga tcaaacagcg gggaaaggat gcggtgcttt ttcattaccc gatggaacgc
     1021 gaagaggccg ccaaatcagg attaaagctt gtgagtatga ccaaataccc tcttcacgac
     1081 ctgctcaagc aggcaggggg caattacctg gaggcgtttg ttttacggct taaattaatg
     1141 cttgaagaaa tgaagctcac ccgcggccga gtggctcttt atggaaaaga cgatatcggt
     1201 cctttttatt caatcctgaa tcgtttacag gaattgatgc ctgaactaca gttcgctggc
     1261 tttgtgcggg acgagatctt gatgaacgcc atgatgacca aagacgcggg tgaaatcgaa
     1321 catatccgcg ccatggggag ggtgaccacc gatgtggtgg cgaagaccgc tgattacatc
     1381 accgggcata aaacaaaggg tggtgtggtg gtacacaaag atggtacgcc ggtgaccatt
     1441 ggcgaggtaa agaacctcat tcaactgtgg ctggcagagc ggggggcgga gaaccccgag
     1501 gggaccattt ttgctattgg cagggacgcg ggtatccccc attcggtggg aaacccgagc
     1561 gacgcgctgc aactggggca gaccatcgtc tacgatatct atccctgtga ggctggcgga
     1621 ggttactatt atgactttac ccgcacctgg tcgctgggct acgtgactga tgaagcgcag
     1681 cagctctatg accaggtcag gcaggtttac gataccatct cagccgagtt aaaagtgaac
     1741 gcaccttttt ataaatttca agaacgtgcc tgcgcgttgt tcgaggagat ggggcacccg
     1801 actatccagt cggatccgac tgtggaacgg gggtatgtgc acagcctggg ccacggcatc
     1861 ggcttgagag tgcatgaaaa gccctgggca tctgcggtca acccttcacc aacagatata
     1921 ttggctcccg ggtctgtgtt tacgcttgaa ccagggttgt attaccctga acgcggtatg
     1981 ggtgtgcgca tcgaagattc gtggtgggtg accccggatg gcgaattcga aatactggcg
     2041 gaattcccta aagacctgct gctgccaatg aaatgaggtg acgtgcagaa agggcgtgtt
     2101 cagtgaaact taaatcagcc cgcttactgg gtattgagac cagctgtgat gaaaccgccg
     2161 cagcggtggt tgagaacgga cgagagatac tttccaatgt ggtcgccact caaatcgacc
     2221 tgcacaccaa atatggcggt gtatttcctg aagtcgcatc gcgccagcat atccgcacca
     2281 tttaccctgt gatcgatgaa gccttgcaaa aggccaacct tggcctggcg gatatcgacg
     2341 ccattgccgt taccagggga cccggcctgg caggatcgct ggtggtgggg atgaatgctg
     2401 ccaagggatt ggcgctgggc agacagctgc cattgattgg cgtgaatcac ctggaagggc
     2461 atatttattc cgcgtggctc taccccgcgg atacgccaaa accacctcca ccgccggagt
     2521 tcccgcaatt ggcgttgctc gtttctggcg ggcatagcga actcaacctg gtacaagacc
     2581 atcttcaata ccagcgcctt ggcggcacac tggatgatgc ggctggcgaa gcctttgaca
     2641 aggtcgcgcg catgctaagg ttgccttatc caggcggccc ggccatccaa caggctgccc
     2701 gagaaggcaa cccccaggcg tttcccttcc cgcggtcaag gcttgaaaac acatggaatt
     2761 tttcgttcag cgggttgaaa accgctgtat tacgcgaggt ccgcgcgctg gaagaggaaa
     2821 accgttcttt gccggtgcca aacctggcag ccagctttca agaagcggtg gtgagcgtgc
     2881 tgttcgaaaa aacaatgaag gcggcgcgtg attacgccgt gaagtccatc ctggtcgctg
     2941 ggggtgtttc ggcgaacgaa gctttacgca agaccttcac ctcgcaaact gaatttccgg
     3001 tgcatatccc accgctttca ctttgcactg acaacgccgc gatgattgcc agcgcgggtt
     3061 attaccattt ttgtttggga catgtagacc cgctggatct ggatgtgctg ccaaactggc
     3121 cgttgagtta agtcactttt tctttgaaag atcatctata tgagtgcagc gagacagttc
     3181 ccttctgagt ttgttgaccg tttgattgcg ggagatgggc aggctttcaa tgaactggtc
     3241 gaccagactt ccgcaggcat ttatgggctg gggttacgca tgctgggaaa cgcgcaagac
     3301 gcggaggata tgctgcagga aacctttctc aaagcgcaca acgcgctgcg agggtttgaa
     3361 aagcgttcct cgctttatac ctggctttac cggatcgcga cgaatgaagc gttaatgatg
     3421 ctgcgcaggc ggaaatcaga atttcatttc gattctgaac cggtaacgca ggaagaagcc
     3481 gagaatggcg aagaggtggt ggattggtgc tgtctgccag aaaaggaatt tatctcagaa
     3541 gaaacaaggt ccgagctcac ccgagctgcc gtgaaacttt cgaacgctct gagaatggta
     3601 tttctcttac gggatgtgcg cggtttttcg accagggaaa cggcggaaat tttacaggta
     3661 aacgaagaag tggtaaaaac ccgcctggtt agagccaggg tgaaactgcg cgacgaattg
     3721 agcggttatt tccgcgaacg gttgcaagga cagggtaata atgggtaaac gcggtcattg
     3781 caaggagcat ttgaggcagt tctcagatta tatcgacgga gaactggcgc ccgacatttg
     3841 cgctcagctt gaggcccatc tacaggagtg cagggactgc caggtggtaa tgaatacgct
     3901 gcaaaagacg atcgacctgt accaggttgc agaaggcgaa gagacgctgc cagaaggggt
     3961 gcgctcgcgc ctgttcgcca gattgcaaat cgctcaatca acagggaaga aacctggtga
     4021 gtgaaaccag cgggatgaac cagcaacccg ggaaatgtcc gtatagcata aatgccccgg
     4081 ctgcggtgcc ctcgcttaaa ggcgagcgct gtcttactgg taaacctggt cagccgtgcc
     4141 cggtatgtgg aaagggcgtt ttggactatg acggttggct ggtgttgggc tgcgcggtgt
     4201 gcgggtttaa agaacccggc ggcagtttca cctgataaca gaaatccttc tccagtttgg
     4261 agagttgggt tcgttttgta aagttgatga gaactgaatt atcacaatca taaccaggag
     4321 gaagcaagaa tggatgcaaa gctgagatgg aaatacgggt tgtctttcaa aggagtcgcg
     4381 gattcaaatt ttgaagtgcc gcttggagct tcggttgatc acggcggagc gggagatggc
     4441 gtggtaccga tggagctgat tttaatggga ttgggaggct gcacggcgat ggatgtcatc
     4501 tcaatccttg aaaagaaacg ccagcaggta actgatttcg aggtgaagct gcacgctgac
     4561 cgcgcgggcg aacaccccaa ggtgttcacg gatatcacca ttgaatatat catcaaggga
     4621 cggaatattg atcccgacgc ggtggcgcgg gcagtcgatt taagtgaaaa caaatattgc
     4681 cccggtatgg cgatgctgcg aaaggcagcc aatattcaaa ccaaatatac gattgaa
//
LOCUS       DAOTLW010000076         4375 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_17172_length_4375_cov_4.600463, whole genome shotgun sequence.
ACCESSION   DAOTLW010000076 DAOTLW010000000
VERSION     DAOTLW010000076.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4375)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4375
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17172_length_4375_cov_4.600463"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="P5516_08420"
     CDS             complement(<1..490)
                     /locus_tag="P5516_08420"
                     /inference="COORDINATES: protein motif:HMM:NF037016.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRT92494.1"
                     /translation="MLSSHASKSLRSMISLFFNVLVVASMVLGSFSTAIAQGSRPSEM
                     ADQAENDLVIKQGSSVRYHAFEGQTLQFDLDVAAPVEGALYTWTLLNPPRNGDAVLSP
                     RGAAVTVAYSALIGFSREDLFTVQVSDTNGQTARISVVVEISVNPGATRSCDYCNKRK
                     SLI"
     gene            complement(1106..1810)
                     /locus_tag="P5516_08425"
     CDS             complement(1106..1810)
                     /locus_tag="P5516_08425"
                     /inference="COORDINATES: protein motif:HMM:NF016119.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HRT92495.1"
                     /translation="MRTRAIMRGRKWVAVVVLAGFCLSACSTPVFTATTSPGLTEEPM
                     VTPTTEPASAAPAQDVEKRPTSAPSSPATGETLAEIARQVYGERLISLIHIPALDVYA
                     YVIPVGWELDIYNADESAWDSPDSLVGWAVSSALPGDDGNILLYGHNNIYSSVFRDLY
                     QLQPGDEVTLITGERQWRYRVAEVVLLPEDEDSGSSALVENLKPTRAPRLTIISCYPP
                     ESNTYRVIVIARPVEG"
     gene            complement(1821..4223)
                     /locus_tag="P5516_08430"
     CDS             complement(1821..4223)
                     /locus_tag="P5516_08430"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HRT92496.1"
                     /translation="MHNIDESVLPITRRSIRTRLAEVLLRVLAACLLLTTIFYFNEQP
                     PTANAQSFTYVPGELIVGLKDGVNSAKFNLIEPLDFALTPKPLVESLNAYIVEVPEGE
                     EEVYRKRLLQSDDVLFVDYNYYITIADTPLVPNDPQWGSQYGPRAVRADYAWNVTTGS
                     PRVIIAVIDTGIDRSHPEFSGRLVAGYDFVQGDSIPQDEHSHGTHVAGIAAATGNNAI
                     GVAGVDWRAKIMAVRVLDAHGDGTLANAAAGIIWAADHGADVINLSISGPSFPSPTIL
                     EDATYYAYRKGVVLVAAAGNYGSNPVTYPARYPWVIAVGMTDSANSRRVESNYGNDLD
                     LMAPGYNILSTMPGGGYGYKSGTSMSAPFVAGAAALLRTDGKFNSPQKIYEALTQTAL
                     DLGAPGKDIYNGYGLLQLDSALSYTPVSEPPVEPVPDVVYELRDSAECTNIPFNWVDI
                     PRTTQNFLGIFGNDSIVTVTLPFPFRYAGNEYSKIRVADNGFISFDLSTSLYDAQRMN
                     GPLPGIGTPQQILAPFWDDLNPSADPEAGIYALTLGTAPARRYVITWHRMPLQAIPTS
                     FVTFQLILHEASGVIDFQYLTTNGPFSTGGSASIGLEYGNGLFGLQVGFNKEGAISNK
                     RALKFYPVEEGIPFDVPGCIKATELPPAPEQNTGQEGERTLESLPFRVYLPEGVPSPG
                     ATLRLRTFTEFTGVSSGMTDLRQYAQLIPDPAQFGWLEPKPRLCYFYTSADVLSAGGH
                     AENLLLAVFDHSTGAWSALSTTLEKSMGAVCAYVPYYSVYGVFGRELPRKLPVTGADL
                     GD"
BASE COUNT         1006 a         1200 c         1197 g          972 t
ORIGIN      
        1 gaattaatga ttttcgttta ttgcaataat cgcaagagcg ggttgcgccc gggttgacgc
       61 tgatctccac cacaacactg atgcgggcag tctgcccgtt cgtgtcgctc acctgcaccg
      121 tgaaaaggtc ctcgcggctg aagccgataa gggcggagta tgccacagtc acagctgccc
      181 ctcgcgggct gagaacagca tcgccgttcc tcggcgggtt gagcagcgtc caggtataaa
      241 gcgcgccctc cactggcgca gcaacatcca ggtcaaactg cagcgtttgc ccctcgaacg
      301 cgtggtagcg gaccgaactt ccctgcttga tgaccagatc gttctccgcc tggtcagcca
      361 tttcggaggg cctgctgcct tgtgcaatgg cagtgctgaa actacccagc accatactgg
      421 caaccaccag tacattaaag aataatgaaa tcatgcttcg taaagattta cttgcgtgtg
      481 aagacaacat ctaaatccct ccattactta gaatagtgta cgtgggtgat aattattctc
      541 gtaagttgtt cctgaatcaa cgaacggaaa ttcttaccga attcagtata gcgcgcgctg
      601 aaaaaaaatc aatagtttta aggaggcaat tttcattatc taaaccgatg gccgggggcg
      661 gatttttcac aagccgttga aagcggcttg tgcctgacgc agcgcgtgtg cataacctcc
      721 gcggttattt cttgatgacc tcagcgtatg atttcaattc gccccttgcc caagagggca
      781 tattcgtaat aaaaagggag agttcgcgag aagcgaactc tcccctgtca ccattggttc
      841 tacctaagat cgtcccggct caccgatcaa tcactgtgaa actgcgcacg gttgaccagg
      901 cgctgtactc tccattggtg ttttctgccc gtacacgcca gtaaagcgtc acatcgccat
      961 caagtggttc tgtggtaatg tgagaagaag acggggatgc gtcttctatc taacctgacc
     1021 acaaaaccaa ttcaaattcc ggcatatggc taatttccac cttgttggct ggcacggtta
     1081 tggttaccgg ctattgacct gctgattacc cctcaaccgg cctggcaatc acaatgacgc
     1141 gataggtgtt gctctctggc ggatagcagg aaataatggt gagccttggc gcgcgcgtgg
     1201 gcttcaggtt ctccactaac gcggaagaac cgctgtcttc atcttctggc agcagaacga
     1261 cctctgccac ccggtaacgc cattggcgtt ctccggtgat caaagtcacc tcatctcccg
     1321 gctgcaactg gtaaagatcg cgaaacacag atgagtagat gttattgtgt ccgtaaagaa
     1381 ggatattgcc atcatcccct ggcagcgcgg aagaaaccgc ccagcccacc aggctgtcgg
     1441 gcgaatccca ggcgctttcg tcagcgttgt aaatatccag ctcccagcca accggaatga
     1501 cataagcgta aacgtccagt gcgggaatgt ggatgagcga gatcaaccgt tctccgtaca
     1561 cctgacgcgc gatttctgcc agggtttcgc cggttgccgg tgaagatggc gctgatgtcg
     1621 gccttttttc gacatcctgt gcaggagcgg cgctggcagg ctcagtggtt ggcgtaacca
     1681 tcggctcctc ggtcaaacca ggtgaagtgg tggcagtgaa gactggcgtg gagcaggcgc
     1741 tcaggcagaa ccctgccaaa accaccaccg ccacccattt gcgcccccgc atgatagccc
     1801 tggttctcat caacggcacc tcaatctccc aggtctgccc ccgtcaccgg cagtttccga
     1861 ggtaattccc tgccaaacac gccatacacc gagtaatacg ggacataggc gcacaccgcg
     1921 cccatgcttt tttccagcgt tgtggaaagc gctgaccagg cgccagtcga gtgatcaaat
     1981 accgccagga gcaggttctc cgcatgcccg cccgcggaga gcacatccgc gctggtgtaa
     2041 aagtaacaca atcgcggttt gggttccagc cagccaaatt gcgccgggtc agggatcagc
     2101 tgcgcgtatt gccgcaggtc ggtcatgccg ctggatacac ccgtgaattc cgtgaatgtt
     2161 cttaagcgca gggtagcgcc cggcgagggc actccctcgg gcagataaac cctgaaaggc
     2221 aggctctcga gcgttctctc gccttcctgc ccggtgttct gttcaggcgc aggtggaagt
     2281 tcggtggctt tgatgcagcc cggcacatca aaggggatac cctcctccac cgggtagaat
     2341 tttaaggccc gtttgttcga aatggcccct tctttgttga atcccacctg caggccaaaa
     2401 aggccgttgc cgtattccag cccgatggag gctgaccctc cagtggaaaa cgggccgttg
     2461 gtggtgagat actgaaaatc aatcaccccg ctcgcttcat gcaagatgag ctgaaaagtc
     2521 acaaaggaag tcgggatggc ttgcaacggc atacggtgcc aggtaatgac gtagcggcgc
     2581 gcaggagccg tgcctaaagt cagggcgtag atgccggctt cagggtctgc cgagggatta
     2641 agatcatccc aaaacggggc gagtatttgt tgtggtgtac cgatcccggg cagcggtccg
     2701 ttcatccttt gtgcatcgta caggcttgtt gacaggtcaa acgagataaa accattatcg
     2761 gcgaccctga tcttggaata ttcgttcccc gcataacgga acgggaacgg aagggttacg
     2821 gttacgatgc tatcgttgcc gaagatacct aagaagttct gcgtagtacg cggaatatcc
     2881 acccagttga acgggatatt cgtgcattcc gctgaatcgc gcaactcgta caccacgtcg
     2941 ggcactggct caaccggtgg ttcgctgaca ggcgtgtaag ataacgcgct gtcaagctgc
     3001 agcaggccat aaccattgta aatatccttg cctggtgcgc caaggtccag cgcggtctgg
     3061 gtgagcgctt catatatttt ttggggggaa ttgaattttc cgtcagtgcg gagcaaagcc
     3121 gccgcgcccg caacaaatgg ggcagacatg gaggtaccgg atttataccc gtaccctcct
     3181 ccgggcatgg tcgagaggat attgtagccc ggcgccatca agtcaaggtc gttgccataa
     3241 ttggattcaa cgcggcggga gttggcagaa tccgtcatac ccaccgcaat aacccacgga
     3301 tagcgtgccg ggtaggtcac cgggttactg ccgtaattgc ccgcggcagc gaccaacacg
     3361 actccttttc ggtaggcata ataagtggcg tcttccagga tggtggggga aggaaaagaa
     3421 ggacctgata tgctcaggtt gatcacatcc gccccgtggt ccgccgccca gatgatgccg
     3481 gctgcggcgt tggccagcgt gccatcgcca tgcgcatcaa gaacgcgcac tgccatgatc
     3541 ttcgcccgcc aatcaacacc cgccacgccg atcgcgttat ttcctgtggc cgcggcgatg
     3601 cccgccacat gcgtgccatg actgtgctca tcctgcggga ttgagtctcc ttgcacaaaa
     3661 tcataaccgg ccaccaaccg cccgctaaat tcagggtgtg agcggtcaat accagtatca
     3721 atcacagcga tgatgactct gggtgagcca gtggtgacat tccaggcgta atctgcccgc
     3781 accgcccgcg ggccgtattg agagccccat tggggatcat ttggcaccag cggcgtatcc
     3841 gcgatggtga tgtagtaatt gtagtcaaca aacagcacat catctgattg gagcaggcgc
     3901 ttgcggtaaa cctcttcctc gccttccgga acttcgacga tataagcgtt gagcgattca
     3961 accaggggtt taggggtcag cgcgaaatcg aggggttcaa ttaaattgaa tttcgcgctg
     4021 ttaacgccgt ccttcaaccc cacgataagc tctccgggca cgtaggtgaa tgattgcgcg
     4081 ttggcagttg gaggctgttc attgaaatag aaaatcgtcg tcaggagaag gcaagccgcc
     4141 agaacgcgga gcagcacctc cgccagccgt gtgcgaatgg accttcgagt aatgggcaaa
     4201 acgctctcgt cgatgttgtg catttacatt aatgctgatt atagtagatt tttactcaac
     4261 gtaaaattca actccagtga tcctgtgagc ggccaggcag catttttcgc tcattcgctg
     4321 gttgatgata ttgagggaaa caagacgagg gtttctaaca agcggcgggt aggat
//
LOCUS       DAOTLW010000077         4369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_17192_length_4369_cov_19.397079, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000077 DAOTLW010000000
VERSION     DAOTLW010000077.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_17192_length_4369_cov_19.397079"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..140)
                     /gene="rpmC"
                     /locus_tag="P5516_08435"
     CDS             complement(<1..140)
                     /gene="rpmC"
                     /locus_tag="P5516_08435"
                     /inference="COORDINATES: protein motif:HMM:NF013027.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRT92497.1"
                     /translation="MNAKEIRELSTEEIKERLMDSRQELMNLRFQAVTGQLTDTSRFK
                     ATR"
     gene            complement(137..562)
                     /gene="rplP"
                     /locus_tag="P5516_08440"
     CDS             complement(137..562)
                     /gene="rplP"
                     /locus_tag="P5516_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HRT92498.1"
                     /translation="MLMPKRVKYRKQQRGRMKGKASRGVEVSFGEYGLKSLECGYLSA
                     RQIEAARRAIVHEMKRRGKVWIRVFPDKPYTKRAAETRMGKGKGAVDHWVAVIRPGRM
                     MFEVGGTDEETAIRALTRARYKLGFKTKIVTREGVGEGS"
     gene            complement(591..1268)
                     /gene="rpsC"
                     /locus_tag="P5516_08445"
     CDS             complement(591..1268)
                     /gene="rpsC"
                     /locus_tag="P5516_08445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HRT92499.1"
                     /translation="MGRKVHPIGFRLGINKPWLGRWYAEGEDYTDQLHQDLRIRELAM
                     KDGERSGVSAVEVERYPGKVKVTLHTSKPGVLIGRKGENVKILRQELEALTGKKVDLD
                     VKEIKSPDTDAFLVAENIAGQLERRISYRRAMKRAIQQAMNHGAEGIKVSVSGRLSGA
                     EMARRVTLREGRVPLQTLRADIDFAKTEALTTYGQIGIKVWIYRGIAVAPAEEKVEAT
                     EGVYVSE"
     assembly_gap    1362..1461
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1462..1685)
                     /gene="rpsS"
                     /locus_tag="P5516_08450"
     CDS             complement(<1462..1685)
                     /gene="rpsS"
                     /locus_tag="P5516_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000124454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HRT92500.1"
                     /translation="MTRSLKKGPYVDPKLLKRIEEMNAKGEKKVIRTWSRSSTIFPQM
                     VGHTIAVHDGHRHVPIYITDNMVGHRLGEFA"
     gene            complement(1717..2556)
                     /gene="rplB"
                     /locus_tag="P5516_08455"
     CDS             complement(1717..2556)
                     /gene="rplB"
                     /locus_tag="P5516_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004924105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HRT92501.1"
                     /translation="MAVKIYKPKTPGQRHKTGYSFEEITKDRPERNLIVPQKKTAGRN
                     AYGRVTVRHRGGGEKRHLRVVDFRREKIGIPAKVAAIEYDPNRTARLALLTYADGEKR
                     YILAPVGVKVGDTLVSGPQAEIRPGNALPLANIPVGTTIHAIELKPGKGAQIGRSAGS
                     SAQLLAKEGKYAHVRMPSGEVRLVLQECMASIGQVGNVDHSNVKLGKAGRARHMGLRP
                     EVRGSAMTPRDHPHGGGEGRSPIGMPGPKTPWGKPALGYKTRRNKSTNKMIIRHRSKA
                     KRR"
     gene            complement(2556..2861)
                     /gene="rplW"
                     /locus_tag="P5516_08460"
     CDS             complement(2556..2861)
                     /gene="rplW"
                     /locus_tag="P5516_08460"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HRT92502.1"
                     /translation="MSYSVFDVLKRPVVTEKSNYQASKLHQYVFEVADYATREMVKTA
                     VETVFDVDVVRVNIINLPAKAHRTSRNRRLALRTSGYKKAIVTLAPEDRIPIFEGVD"
     gene            complement(2877..3509)
                     /gene="rplD"
                     /locus_tag="P5516_08465"
     CDS             complement(2877..3509)
                     /gene="rplD"
                     /locus_tag="P5516_08465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HRT92503.1"
                     /translation="MMKVDVYNLQGEKSSTVDLPEEIFAAKVNVDLMHQAFLRQMANA
                     RLGTHSTKRRGEVAGGGAKPWRQKGTGRARQGSTISAQWVGGGRIHTPKPRDHSQAMP
                     KKMRRAALRSALTVKVNDNSVIVVSDLALQEPKSAELSKAIKALAGDSRVLILIPDKS
                     EANQNAIKSGRNLAEHKLLLANYLNIRDLLQYEKVIIPLASLEVIKSYLG"
     gene            complement(3536..4168)
                     /gene="rplC"
                     /locus_tag="P5516_08470"
     CDS             complement(3536..4168)
                     /gene="rplC"
                     /locus_tag="P5516_08470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HRT92504.1"
                     /translation="MFKGLIGRKIGMTQIFDENGAAVPVTLLEAGPCFVSQVKTVATD
                     GYNAVQLAFGEVKPKRVSKAHLGHLEKNNLPPMRVLREFRIKNVEEIKAGDVLNASVF
                     EAGEHVDVIGISKGKGFAGAVKRHGFRGGPRTHGQSDRMRAPGSSSATTTPGRVFKGK
                     RGPGRMGNESVTSSNIRVVLVDPERNLIAVSGSVPGPKGGTVVIKEARKQ"
BASE COUNT          935 a         1248 c          991 g         1095 t
ORIGIN      
        1 cgggtggctt taaatctgct ggtatccgtc agctgaccgg tcaccgcctg aaagcgaaga
       61 ttcatcagtt cctgccggga atccattaaa cgttctttaa tttcttctgt agaaagttct
      121 cttatttctt tagcgttcat gaaccttctc ctacaccttc acgggtcaca attttcgtct
      181 tgaaacccaa cttatagcgg gcacgcgtga gtgcgcggat ggcggtttcc tcatcggtgc
      241 cgccgacttc aaacatcatg cgccctgggc ggataactgc cacccaatgg tcaacggcgc
      301 ccttaccctt acccatacgg gtttcagctg cgcgtttggt ataaggctta tctgggaaaa
      361 cccgaatcca tactttgcca cggcgcttca tctcgtgcac gatagcacgg cgcgctgctt
      421 caatttggcg ggcactcaga tagccacatt ccaatgactt cagaccatat tcaccgaagc
      481 tgacttcaac cccacggctg gctttgcctt tcatgcggcc gcgttgctgc ttgcggtact
      541 taactctctt tggcattaac ataattcaat acctcaccat aaattctcaa ctactcgctg
      601 acgtatacgc cttcagtagc ttccactttt tcttcagcgg gagccacggc gatgccacga
      661 taaatccaaa ccttaatgcc gatttgaccg tatgtcgtca aagcctcggt cttggcgaag
      721 tcgatatccg cgcgcaaggt ctgcaggggc acacgccctt cccgcaaagt cacgcggcgt
      781 gccatttcag cgccagaaag ccggcctgaa accgaaacct taatcccttc agccccgtgg
      841 ttcatcgctt gctgaatggc gcgcttcatc gcacggcgat aactaatacg ccgctccaat
      901 tgaccagcga tattttccgc gactaaaaaa gcatccgtat ctggagattt gatctcttta
      961 acatcgagat caactttttt gccggtgagc gcttcgagtt cctgacgtaa gattttcacg
     1021 ttttcgccct tgcgtccaat caacacgcca ggcttcgagg tgtgcaaggt aaccttcacc
     1081 ttgcccggat agcgttccac ttcaactgcc gaaacgccag atcgctcgcc gtctttcatc
     1141 gccaattcgc gaatgcgcaa atcttgatga agttgatcgg tataatcctc accttcggcg
     1201 taccagcgcc ctaaccaggg cttattaatc cccagacgaa aaccaattgg atgtacttta
     1261 cgacccataa ttactcctca actctctttc cagtcctact cattttcacg caaaattacg
     1321 gttacgtgag agctgcgtcg cagcagcggc ttgaaacggc cnnnnnnnnn nnnnnnnnnn
     1381 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1441 nnnnnnnnnn nnnnnnnnnn ngcaaattcg cctaagcggt gaccaaccat gttatcggtt
     1501 atgtaaatcg gtacatggcg gtgaccgtca tgcacggcaa tggtatgacc aaccatctgg
     1561 gggaaaatgg tgctcgagcg gctccaggtg cgaatcacct tcttctcgcc tttagcgttc
     1621 atttcctcga tgcgtttcaa aagttttggg tctacatacg gccctttttt caatgatcgc
     1681 gtcatgttaa tatccttttc tacctatcac caatttctaa cggcgcttag cctttgagcg
     1741 gtggcgaata atcatcttgt tcgtactctt gttccgcctg gtcttataac ccaaagctgg
     1801 tttaccccag ggtgttttcg gaccgggcat accaatcggg ctgcggcctt caccaccacc
     1861 atgcggatga tcccgagggg tcatggcgga gccgcgtact tctgggcgca agcccatgtg
     1921 gcgtgctcgt ccggctttcc ccagcttcac gttgctgtgg tccacatttc ctacctgccc
     1981 aatagatgcc atgcactctt gcaaaactaa gcgcacctca ccagaaggca tacgcacatg
     2041 tgcatacttg ccttccttag ccagcaactg cgcagaagag cctgccgagc ggccaatttg
     2101 agcgcccttg ccaggcttca gctcaatggc atgaatggtc gtgccgaccg gtatgtttgc
     2161 cagcggcaga gcattacccg gacgaatttc ggcttgcgga ccagagacca gcgtatcgcc
     2221 tacttttaca cccacgggcg caagaatgta gcgcttttcg ccatcggcat aagtgagcaa
     2281 cgccaagcga gcggtacgat tgggatcata ctcaatcgct gctaccttag cagggatgcc
     2341 aattttctcc cgacggaagt ccaccacgcg caggtggcgt ttttcaccac caccacggtg
     2401 ccgtacagtg actcgaccgt aggcgttgcg tccagccgtt ttcttttgag gcacgatcag
     2461 attgcgttct gggcggtctt tggtgatttc ctcaaaagag tagccagtct tatggcgttg
     2521 accaggggtc ttcggtttat aaattttgac agccattagt ccaccccttc aaaaatcggg
     2581 attctatctt ccggtgccaa ggtgacaatc gcttttttgt aaccgctggt gcgcaatgcc
     2641 agccggcggt tccgcgaagt gcgatgtgct ttagcaggaa gattgataat atttacacgc
     2701 accacatcca cgtcaaacac ggtttcgacg gcggtcttca ccatctcacg cgtggcatag
     2761 tccgccactt cgaacacgta ttggtgcagc ttgctcgcct gatagttaga cttttcagtc
     2821 acaaccgggc gtttcaaaac atcaaaaaca gagtagctca ttctgcttca ccttccttat
     2881 cccaaataac tcttgatcac ctcaagagag gcgagcggga taattacttt ttcatactgc
     2941 aataaatccc gaatattcaa ataattcgcc aacaacagct tgtgttcggc aaggttgcgg
     3001 ccagatttaa tcgcattttg gttggcttca cttttatccg ggatcaaaat caacacgcgg
     3061 ctatcaccag ctaacgcttt aatcgctttg ctcaattccg cagattttgg ctcttgcagc
     3121 gcaaggtcgc ttaccacaat cacgctgtta tcattgacct tgacggtcag tgcagagcgt
     3181 aaggctgcgc ggcgcatctt cttaggcatt gcttgggaat gatcccgcgg tttgggtgta
     3241 tgaatacgcc caccgccgac ccattgggca gagatcgtgc tgccttggcg ggcacgtccg
     3301 gtgcccttct ggcgccaagg cttggcgccg ccgccagcca cttcaccgcg ccgcttggtg
     3361 ctatgcgttc ccaatcgcgc attcgccatc tggcgtaaaa atgcctggtg catcaagtca
     3421 acattgacct tcgccgcgaa gatttcttcc ggcaaatcca ctgtgctgga tttttcacct
     3481 tgtaagttat aaacatcaac tttcatcatt gcgttctcca ttaccacaag ccggcttact
     3541 gcttgcgagc ttctttgata accacggtgc cgcccttagg tcctggcacc gagccgctca
     3601 cagcaatcag gttgcgctca gggtccacca agaccacacg gatgttggat gaggttacgc
     3661 tctcattacc catcctgccc ggaccgcgct tgcccttgaa gacccgcccc ggagtcgtgg
     3721 tcgcagagga agaacccggc gcgcgcatgc ggtccgattg accgtgcgtg cggggaccgc
     3781 cgcggaaacc atggcgctta actgcaccag cgaaaccttt ccccttgctg atgccaatca
     3841 cgtccacgtg ttcacctgcc tcaaacaccg aggcattcaa cacatcacca gctttgattt
     3901 cttctacatt tttgatgcgg aattcacgca gaacccgcat gggaggcagg ttgttctttt
     3961 ccagatgacc taagtgcgct ttgctcacgc gcttgggctt cacctcgccg aaggcaagct
     4021 gcaccgcgtt gtagccatcg gtggcaaccg tcttgacctg ggaaacaaaa caaggcccag
     4081 cttcgagtaa cgttaccgga acggcagcgc cgttctcatc gaagatctgg gtcataccga
     4141 tctttcttcc aattagccct ttaaacatac tattctcctg ttagttaatc ttggcgagac
     4201 tgtcagcatg ggctggctgc atcatctccg cgcaattcag ttaatatgcc ctgtgtagct
     4261 ttgctgccca cgcgggtcac attcttaaac tgcctactta cttatattgc cttggggggc
     4321 aaccttgcaa atataccaca cctcccttgc agaatgcaag ggcaaaatg
//
LOCUS       DAOTLW010000078         4111 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_18451_length_4111_cov_14.441815, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000078 DAOTLW010000000
VERSION     DAOTLW010000078.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_18451_length_4111_cov_14.441815"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1770
                     /locus_tag="P5516_08475"
     CDS             <1..1770
                     /locus_tag="P5516_08475"
                     /inference="COORDINATES: protein motif:HMM:NF021436.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2142 domain-containing protein"
                     /protein_id="HRT92505.1"
                     /translation="ILFGVGLAVFAPFGTGFDEDKHLARVFDISGLHMLPNRSIYDKT
                     AFFSEFHTLSYRRFFYRDQGAELFTPAYFSVKGNYGSMAVETTMSTYPPLMFFPQAGV
                     AGIAWRVKDLPIIPVVIVIRLVTLAVYLLGCYLAIRTTPVAKWLFLVAALLPTPLYQA
                     STVNGDGYTHAVSLLFIAVVLSVVFQTEATISRGRFLSLCAAVFFLAMAKPGSILLLP
                     LLLLIPGRVFTTRSQRLGLILLTAGMSVFHLVWIYYSFTNTSLGVESTRTVVSSVLSQ
                     LWAYLSAFWRSLLFHWRTLFGSMIAASGYWGTYVSPLVYGLVGAVLLVAILFTGHDKR
                     FRTRTRLFFIGMLLLTWFGILVMYTAGKFTYGEKETMAMVQGRYLVPFLLLGLLAVWG
                     IFPRLRQQASWAGWFIGAGIVAFQALFFWGIYAHYYTPCGPSLFSGRNCRLPAYQNIE
                     TSIPPEVPLEQGVVLEQRFTNTCRRLAAVAVLVDDGAANARGGVELALNTEQGRVVAR
                     AELDAAELTPLKRWTLDLPPGTELARGEYSITLRGEQLNGAVPFAVRTPDRYPGALTL
                     GGQPFDGDLVFYYYCAPVGLFGY"
     gene            complement(1800..3092)
                     /locus_tag="P5516_08480"
     CDS             complement(1800..3092)
                     /locus_tag="P5516_08480"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92506.1"
                     /translation="MKNPDAFKRAARVSTILFFIFMLLHQTDKLLIGPLQTQVMETFH
                     MTYTQWGAINTGALLVGAILYPVWGWLNDRYNRGKLLALASLIWGSTTWLSAIVKTFP
                     AFLVTRSSTGIDDSSYPGMMSLLADYYPPKSRGKVYGILQLTQPIGYLLGMVLALMLG
                     GVLGWRFIFYITGSLGVVLAIAIFFGVKDVPRGSSEEELHNVEKSKYKFNWKTAIAIF
                     KKRSLWFVFAQGFIGVFPWNVITYYFFGYLSTDRGYDSTTQLLIMAPAVLFMAAGYPL
                     GGALGDRLFKRTQRGRLIAGAIGIFMGAVLLYFTIHTPNPQVFLFAALLMGTALFMPF
                     ASPNIISTIYDVTLPEVRSSANAIQNFIEFIGSAAAPLIAGIIADKFSVGTAILSICL
                     TAWALCFLFILVAIALIPKDINAMHRQLQERAEAEKVA"
     gene            complement(3201..>4111)
                     /locus_tag="P5516_08485"
     CDS             complement(3201..>4111)
                     /locus_tag="P5516_08485"
                     /inference="COORDINATES: protein motif:HMM:NF024183.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HRT92507.1"
                     /translation="WRKMAKVITHKCNACGAPLSVSGRGGKYTCPHCGMINLFEAEES
                     SADSFICPQCGTANPKEALHCNECGMKFTLTCPKCGAVNAADSLFCLKCGVNFQSEFA
                     QRESAQARALLQQQQAQVKSSKRKKVVGVFAAVLGLLLLLCVALGMYALKFSPEARAT
                     ATAEALTRQQATQTFFETLKAQYPFHGSNGAYAVYLKNFCIEHDQNSGRDFAWIRLRY
                     FQEDPSYDFDWDFKRTYLTDDLGNRFEVTQEMFYGNDGKNMVTDFDPASSSLVLHVIS
                     DAWALPEIQISFTPNDPILTATCADE"
BASE COUNT          821 a         1080 c         1281 g          929 t
ORIGIN      
        1 attctctttg gggtgggatt ggccgttttc gcccctttcg gcactggatt tgacgaggat
       61 aaacacctgg cgcgggtgtt tgatatctcc gggctgcaca tgctgcctaa ccgcagcatc
      121 tatgataaga cggcgttctt ctctgagttt cacaccctgt cttacaggcg ctttttctac
      181 cgcgaccagg gcgcggaact gttcacccct gcgtacttta gtgtgaaggg taactatggc
      241 agcatggctg tcgaaaccac catgtctacc tatcccccgt tgatgttctt cccgcaggcg
      301 ggggtggcgg gtatcgcatg gcgcgtgaag gacctgccga tcataccggt ggtgatcgtt
      361 attcgccttg tcacgctggc ggtatacctg ctcggctgct acctggccat ccgcaccacg
      421 ccagtggcga aatggctctt tctggtggcg gcgcttttac ccacgccgtt ataccaggcg
      481 tccaccgtca atggagatgg ctacacccac gcggtgagtt tactgtttat cgccgtcgtg
      541 ctctccgtag tgtttcaaac cgaggcaacc atctcccgcg gacgtttctt gagcctgtgc
      601 gctgcggtct tttttctggc gatggccaaa ccagggagca tcttattgct gcccttgctg
      661 ctgctcattc ccgggcgcgt tttcaccacg cgctcccagc ggcttgggtt gattttgctg
      721 accgcgggga tgagcgtctt tcacctggtg tggatctact acagcttcac gaacaccagc
      781 ttgggagtgg aatcgacgag aacggttgtt tcaagcgtgt tatcacaatt atgggcttat
      841 ctgagcgcgt tctggcggtc gctgcttttc cattggcgca cgttgtttgg ctcgatgatc
      901 gccgcgtcgg gctactgggg aacctatgta tccccgctgg tttacgggct tgtgggcgcg
      961 gttctgcttg ttgccatcct ttttaccgga catgataagc gtttccgcac gcgaacgcgc
     1021 cttttcttta tcggtatgct gctgctgacc tggtttggga ttttggtgat gtacaccgcg
     1081 gggaaattca cttatggtga aaaagaaacc atggcgatgg tccaggggcg ctatctcgta
     1141 cctttcttac tacttgggct tttagccgtg tgggggattt tcccccgctt gcgccaacaa
     1201 gcaagttggg cgggctggtt tattggcgct ggtatcgtgg cttttcaagc gttgttcttc
     1261 tgggggatat atgcccatta ttacacccct tgcggaccca gcctgttcag cgggaggaac
     1321 tgcaggttgc ccgcttacca gaatattgaa acatccatcc cacccgaggt gccgctggag
     1381 cagggggtgg tgctggagca gcgcttcacc aatacctgcc ggcgcctggc ggcggtggcg
     1441 gtgctggtgg atgacggagc agcaaacgcg cggggcgggg tagaattagc gctgaacacc
     1501 gagcagggca gggtggtggc gcgcgctgag ctggacgccg ctgagctcac gccgctgaaa
     1561 cgttggaccc tcgacctgcc gccggggacc gagctggcgc gaggggagta cagcatcacg
     1621 ctgcgcgggg agcagttgaa cggcgcggtg cctttcgctg tgcgcacgcc tgaccgttac
     1681 cccggggcgc tcacgctcgg cggacaacct tttgacggcg acctggtatt ctactactac
     1741 tgcgcgccgg tgggattgtt tggctattga gccgcgtgat aaagagccgc gtgttgtttt
     1801 taagcgactt tttcggcttc cgcgcgctcc tgcagctggc ggtgcatggc gttgatgtct
     1861 ttggggatga gcgcgatggc cacgaggatg aacaagaagc acagcgccca ggcggtaagg
     1921 cagatggaca ggatggcggt gcccacgctg aacttatcgg cgatgatgcc ggcgatgagc
     1981 ggcgctgccg cggaaccgat gaattcaatg aaattctgaa tggcgttggc gctggagcgc
     2041 acttccggca gggtgacatc gtaaatggtc gaaatgatat tcggagaggc gaagggcatg
     2101 aagagcgccg tgcccatcag cagggcggcg aagaggaaga cctgggggtt gggcgtgtgg
     2161 atggtgaagt acagcagcac cgcgcccatg aagatgccga tggcgccggc gatcaggcgt
     2221 ccgcgctggg tgcgtttgaa gaggcggtcg ccgagcgcgc cacccagcgg gtagcctgct
     2281 gccatgaaga gcaccgccgg cgccatgatg agcagctggg tggtgctgtc ataaccgcgg
     2341 tcggtggaaa ggtagccgaa gaagtaatag gtgatcacgt tccaggggaa gacgccaatg
     2401 aagccctgcg caaacacaaa ccacaggctg cgcttcttga agatggcgat ggcagttttc
     2461 cagttgaatt tgtacttgct cttttccaca ttatgcagtt cttcttcgct gctgccgcgc
     2521 ggcacgtctt tcacgccgaa gaatatggcg atggccagca ccacgcccag tgagccggtg
     2581 atgtagaaaa taaagcgcca gccaagcaca ccgccgagca tgagcgccag caccatgccc
     2641 agcaggtagc cgataggctg ggtgagctgg agaataccgt agaccttgcc gcgcgacttg
     2701 ggcgggtagt agtctgccag caggctcatc atgcccgggt agctggaatc atcaatgccg
     2761 gtggaagaac gcgtgaccag aaaagccggg aaggttttga caatggcgga gagccaggtg
     2821 gtagagcccc agatgagcga ggccagcgcc agcagtttgc cgcggttgta gcggtcgttg
     2881 agccagcccc acaccgggta gaggatggcg cccaccagca gcgcgccggt gttgatggcg
     2941 ccccactggg tgtaggtcat atgaaaagtc tccatcacct gggtctgcag cggaccgatg
     3001 agcagcttat cggtctggtg cagcagcatg aagatgaaga acaagatggt ggatacacgc
     3061 gcggcgcgtt tgaacgcatc aggatttttc atcgaagaat caccttccta tgcagagaaa
     3121 ataacaacct gtatacctgg tattgtacag gaaaggcgaa aagaggggag atgctccttt
     3181 cgcgcccgcc gttcccgcct tcattcatcc gcgcaggtag cggtgagtat cgggtcgttc
     3241 ggcgtgaatg agatttgaat ttcgggcagc gcccaggcgt cgctgatgac gtgcaagacc
     3301 agtgaagaag aagcggggtc aaaatcagtg accatgttct tgccgtcatt gccgtagaac
     3361 atttcctggg tgacctcgaa acgattgccc aggtcatctg tgaggtacgt tcgcttgaaa
     3421 tcccagtcaa aatcatagga ggggtcctct tgaaaatagc gcaaacggat ccaggcgaaa
     3481 tcacgccctg aattctgatc gtgttcgatg caaaaatttt tcaggtaaac cgcataagcc
     3541 ccgttgctgc cgtggaaagg gtactgcgct ttcaaggttt cgaagaaggt ttgggtggct
     3601 tgctgcctgg tcagcgcttc agcggtggcg gtggcgcgcg cttcaggaga gaatttgagg
     3661 gcatacatgc caagggcaac gcataataat aagagcagac cgagaaccgc ggcgaaaacc
     3721 ccgacaactt ttttcctttt gctggattta acttgcgcct gctgctgttg caggagagcc
     3781 cgggcctgcg ctgattctcg ctgagcgaac tcgctttgaa aattgacccc gcatttcagg
     3841 cagaagagcg agtcggcggc gttgaccgcg ccgcatttcg ggcaggtgag ggtgaacttc
     3901 atgccgcatt cattgcaatg cagcgcctct ttggggttgg ctgtgccgca ttgagggcaa
     3961 ataaaagagt cagcggaact ctcttcagcc tcgaagagat tgatcatccc gcagtgaggg
     4021 caggtgtatt taccgccacg cccggatacg ctcagcggag cgccgcaagc attacattta
     4081 tgggtgatga cttttgccat ttttctccat t
//
LOCUS       DAOTLW010000079         3980 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_19164_length_3980_cov_17.201529, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000079 DAOTLW010000000
VERSION     DAOTLW010000079.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3980)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3980
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19164_length_3980_cov_17.201529"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /gene="rpsJ"
                     /locus_tag="P5516_08490"
     CDS             complement(<1..239)
                     /gene="rpsJ"
                     /locus_tag="P5516_08490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014268624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HRT92508.1"
                     /translation="MAKQRIRIRLKAYDHRILDQSAKRIVETAERSGAQVVGPVPLPT
                     RIERYTVRRSTFIDKDSHEHFEIRTHNRLIDVLDP"
     gene            complement(312..>2008)
                     /gene="fusA"
                     /locus_tag="P5516_08495"
     CDS             complement(312..>2008)
                     /gene="fusA"
                     /locus_tag="P5516_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HRT92509.1"
                     /translation="VPRICFANKMDRKGASYTRTVESIRSRLGAEPIAMQIPIGEEAA
                     FEGVINLLEEEAVYFEDELGKEPIVKPIPAEYQEEAAQKRQALIEQIAELDDELLQKY
                     LDDEPLSLPELKAALRKGVIANKVTPVFCGTSLRNKGVQPLLDAVVDYLPSPLDIPPV
                     KATLLKDGSEVLRHPDSSEPLAALVFKIVTDPYMGRLAYVRVYSGKITQGGAVYNSSK
                     DKKERIGRLLRMYADRREDIPELGAGDIGAILGLKFSFTGDTLSDPSAPVLLESITFP
                     DPVISVAIEPKTKADQEKMAESLQKLSEEDPTFKIRQDENTGQTVISGMGELHLDVLV
                     DRLLREFKVQANVGKPKVAYRETILRPVENVNYKYTKQTGGHGQYGHVVINMEPLPNG
                     TGIQFENKIRGGVIPKEYIPAVEKGIYEAAESGPLAGYPMTDFKVTLVDGSYHEVDSS
                     ELAFRMAAIFAFREAAEKATPVLLEPIMKVEVTVPEEYTGDVLGQINARSGNVLGMEQ
                     QFGNAQLIHAEVPLSEMFGYATELRSATQGRGVFTMEFKHYAQVSESKKKAILGER"
     assembly_gap    2009..2108
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2240..2656)
                     /gene="rpsL"
                     /locus_tag="P5516_08500"
     CDS             complement(2240..2656)
                     /gene="rpsL"
                     /locus_tag="P5516_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018003268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HRT92510.1"
                     /translation="MPTINQLVRKGRKTKQQKSKSPALQYTLNAKKQRRTRQPKGAPQ
                     KRGVCTVVRTMTPKKPNSALRKIARVRLSNGMEVTAYIPGEGHQLQEHSVVLVRGGRV
                     KDLPGVRYHVVRGALDSTGVADRKRGRSKYGAKRGK"
     gene            complement(2796..>3980)
                     /locus_tag="P5516_08505"
     CDS             complement(2796..>3980)
                     /locus_tag="P5516_08505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HRT92511.1"
                     /translation="MAGDKMAGRHGNKGVVSMVVPVEDMPFLEDGRPVDIILNPLGIP
                     GRMNIGQVLETHLGWAANSLGFRAITPVFDGASESEIEAELARAWLIDEAWRITGEKA
                     WEWLNSVGYEPEQIKDDEEARLIYLANRLGDKGYDIFTLQENNVVARRTAMKEWLESE
                     GYDPKLITAFPEDNIPVKEREVQDERAVRACLELWAKRLGRQLPGDLSTEELKKAAQT
                     IMVETNTPTPIIGKQVLRDGKTGEAYDQPVTVGYMTFLKLHHLVEDKVHARSTGPYSL
                     VTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTLQEMLTIKSDDVAGRDATYQAIVSN
                     KPIEAPGLPSAFKVLVKELQSLGLEVESIMEDGEIETYGKDSDKRDTRIPTGLLDSPF
                     GA"
BASE COUNT          836 a         1085 c          932 g         1027 t
ORIGIN      
        1 tcggggtcca gcacgtcaat caggcggttg tgggtgcgga tctcgaagtg ctcatgcgaa
       61 tccttatcaa taaaggtgga gcggcgcacc gtgtagcgct cgatgcgcgt gggcagcggt
      121 accggtccaa ccacctgagc gccactgcgt tcagccgtct ccacaatccg cttagcggat
      181 tgatccagga tgcggtggtc ataagctttc aagcgaatac gaattctttg ttttgccata
      241 atcttcctaa atcagttcta atttttagtt ctctccagca gtttcctgcc agaggcgtta
      301 gcctatttca attatctctc gcctaaaatg gctttcttct tgctctccga aacttgggca
      361 tagtgtttga attccatcgt aaacacgccc ctgccttgcg tagcagagcg cagttccgtg
      421 gcatacccaa acatctccga gagcggcact tcagcatgga ttagctgagc gttcccaaac
      481 tgttgctcca tgcccaaaac gttgccagag cgggcattaa tctggcctaa aacatcacct
      541 gtatattctt ccggcacggt gacctcaacc ttcatgatgg gttcgagtaa aaccggtgtt
      601 gctttttctg ccgcctcgcg gaaggcgaag atcgctgcca tgcggaaagc caactcacta
      661 gaatccacct cgtgataaga cccatccacc aacgttacct tgaagtcggt catggggtag
      721 ccggctaagg gtccgctctc agcagcttcg tagattcctt tttccaccgc cggaatatac
      781 tccttgggga tgacgccccc gcggattttg ttttcgaatt ggattccggt gccatttggg
      841 aggggttcca tattaatgac cacatgccca tattgcccgt gaccgccggt ctgcttggta
      901 tatttatagt tgacattttc caccggtcga aggatggtct cccggtatgc cactttgggt
      961 ttacctacgt tggcttgcac cttgaattcg cgcagcaggc ggtcaactaa cacgtccaaa
     1021 tgcagttcgc ccattccgga gataaccgtc tgaccggtat tttcgtcctg gcggattttg
     1081 aaggtggggt cttcttccga aagcttctgc agcgattcag ccatcttctc ctgatcggct
     1141 ttggttttgg gctcgattgc caccgaaata accgggtcgg ggaaggtgat gctttccaat
     1201 aacacaggag cagaagggtc gctaagcgta tcaccggtaa agctaaactt cagccctaat
     1261 atggcgccga tatcgccggc acctaactcg gggatgtctt cgcgcctatc ggcgtacatg
     1321 cgcagcaacc gcccgatgcg ttctttctta tcctttgagg agttatagac tgctccgcct
     1381 tgagtaattt tcccggagta gacgcgcacg taagccaacc tgcccatata ggggtctgta
     1441 acaatcttaa aaaccaaagc agccaggggt tcgcttgagt cggggtgacg taacacttca
     1501 ctgccgtctt ttagcagtgt ggcttttacg gggggaatat ccaatggtga gggcagatag
     1561 tccaccacag catccaaaag gggctgcacg cctttattgc gtaagctcgt gccgcaaaag
     1621 acgggggtaa ctttattggc aatcacacct ttgcgtaaag ccgcttttag ctcaggcaag
     1681 ctcaggggtt catcatctaa gtatttctgt aagagctcat catccagctc agcgatctgc
     1741 tcgattagag cttggcgttt ttgggcggct tcttcttgat attcagctgg gataggcttg
     1801 actatcggtt ctttgcctaa ttcgtcttcg aagtaaactg cttcttcttc cagcaggttg
     1861 atcacaccct cgaaggctgc ttcttcgcca atggggatct gcatggcgat tggctcagcg
     1921 cctaagcggc tccgaatgct ctcgacggtg cgggtatacg aagcgccttt gcggtccatc
     1981 ttattggcaa agcatatccg cggcacctnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2101 nnnnnnnngt aacgcacatc cggcggaact tcgcgttttt cgggtttaaa tcttcttgcc
     2161 ataattgctc ctcattaaaa tgcccaaggc aatcgcgttc aacgggtgag cgcctgcctc
     2221 atctcatttt catccagtgc tatttaccgc gtttagcgcc gtatttggaa cgtccgcgct
     2281 tacggtcagc cacgcctgtc gaatctaacg caccgcgcac aacatgatag cgcacgcctg
     2341 gcaggtcttt cactctgccg ccgcgcacca acaccacgga gtgctcctgc agctgatgac
     2401 cttcgccggg gatgtaagcg gtaacttcca tcccattgga aagacgcaca cgggcaatct
     2461 tccgcaaggc tgagttcggc tttttgggcg tcatcgtgcg cacaacagtg caaacgccgc
     2521 gtttctgcgg ggcaccttta ggttggcgcg tacggcgctg cttctttgcg ttaagcgtgt
     2581 attgcaaagc aggcgacttg cttttttgtt gcttagtctt gcgacctttg cgaactaatt
     2641 gattaattgt tggcacattg cctccgtcca gtttctataa attaccaatt attcatcaat
     2701 caaggccatc aatctaacct gatggccttg ttcattacga ctttcgccaa acaaggctta
     2761 cgaaaccctg cctggcaggg cgaggattga ttttatcacg ccccaaatgg tgagtcaagt
     2821 aaacctgtcg ggatgcgcgt gtcgcgtttg tcactatcct taccataagt ctcaatctcg
     2881 ccatcttcca tgatggattc gacctctaac cctaagctct gcagttcctt gactaaaact
     2941 ttgaatgccg aaggtaaacc tggggcttca atcggtttat tggagacaat agcttggtaa
     3001 gttgcatcgc gccccgccac gtcatcggat ttaattgtga gcatttcctg cagggtgtag
     3061 gcggcaccat aagcctccag cgcccagact tccatctcgc caaagcgctg tccgccaaat
     3121 tgtgcctttc cacccaaggg ctgttgcgtc accaagctgt atggaccagt cgagcgcgcg
     3181 tgcaccttat cctcgaccaa gtggtgcagc ttcaaaaagg tcatatatcc tacggtgact
     3241 ggttggtcgt atgcctcacc agtcttcccg tcgcgcaaga cttgctttcc aataattggg
     3301 gtaggcgtat tggtttccac catgatggtc tgagcagctt tcttaagttc ttccgtgctc
     3361 aagtcaccag gcagctgcct gccgaggcgc tttgcccaga gttctaagca tgcacgcacc
     3421 gcgcgctcgt cctgcacctc gcgttccttc accggaatat tatcttctgg gaaggcagtg
     3481 atcagttttg gatcgtatcc ttcgctctct aaccattctt tcatcgccgt gcgacgggca
     3541 acaacattgt tttcctgcaa ggtgaaaata tcgtagcctt tatcccctaa gcgattcgct
     3601 aagtaaatca ggcgggcttc ttcgtcatct tttatctgct caggttcata gccaacggaa
     3661 ttcaaccatt cccatgcctt ctcaccagtg atgcgccaag cttcgtcgat caaccaagcc
     3721 cgcgcgagct cagcctcaat ttcactttca gaggcaccat caaagaccgg ggtaatcgcc
     3781 cggaagccta aactgtttgc agcccaacct aagtgcgtct ccaaaacctg gccgatgttc
     3841 atacgcccag ggatgcctaa cgggttcaag ataatatcta ctggtctgcc atcttccaag
     3901 aaaggcatat cttcgacggg tacgaccatc gaaaccacac ccttattccc atgccgccca
     3961 gccatcttat cgccagccat
//
LOCUS       DAOTLW010000080         3934 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_19421_length_3934_cov_10.269399, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000080 DAOTLW010000000
VERSION     DAOTLW010000080.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3934)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3934
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_19421_length_3934_cov_10.269399"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(157..1035)
                     /locus_tag="P5516_08510"
     CDS             complement(157..1035)
                     /locus_tag="P5516_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRT92512.1"
                     /translation="MPNGEEFNPRKLDTPLDKIMRTSAGRRSVTRTERKRGRYVQSRP
                     AQGKTDDLAFDATVRAAAPFQKERAEIKERHRVAFAVKPADYMKKVRVRKAANLILFL
                     VDASWSMAVAERMAATKGAILSLLTDAYQRRDRVGLIVFQKNLATQVLSPTNSVMLAE
                     AALKDIPVGGKTPLSAGLMMAYEVLRKEKILHPEVMPLLIVLTDGAGNVSIGFSSPQE
                     EAYHIADQIAKENIHSVVVNMEHAAFDQGLAQSLADHLKAPCYTITDLKAESLYVTVQ
                     QEIRQVNASSNIELKK"
     gene            complement(1143..2246)
                     /locus_tag="P5516_08515"
     CDS             complement(1143..2246)
                     /locus_tag="P5516_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT92513.1"
                     /translation="MPAIYPFTAIVGQERMRRALVLNAVDPRIGGVLIRGERGTAKST
                     AARALAALLPSVKVVAECRFGCDPEKPATWCTECRERAERSEKLEAITRHTPFVNLPV
                     SATEDRVVGTLDIEKAIQKGVRSFEPGVLAAANRGLLYIDEVNLLDDHVVDVLLDAAA
                     MGVNIVEREGISFTHPARFILVGTMNPEEGDLRPQLLDRFALSVEIRGIRDARERVQI
                     MERNIAFEEDPKLFYDSWEEKEQELSHQIENARKLVDEVTYTRQNLLSIAALTASLNV
                     DGHRSDLVILKTSRAQAAFDGRRQINDRDIALAAELALPHRLKSGPFQREEMGMEELQ
                     ERIEQLQGGTSGENEEETISELGGEETGSPKKA"
     gene            complement(2442..2762)
                     /locus_tag="P5516_08520"
     CDS             complement(2442..2762)
                     /locus_tag="P5516_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRT92514.1"
                     /translation="MTTPPSNYEQGVPVFPIVKRESSLAIISLVAGIIGWTIIPFIGS
                     VIAVITGHLAKKEIRESGNTMSGDGMALAGLILGYTMIGISLLAILVVVLAFLLIARS
                     ELGL"
     gene            complement(2869..3201)
                     /locus_tag="P5516_08525"
     CDS             complement(2869..3201)
                     /locus_tag="P5516_08525"
                     /inference="COORDINATES: protein motif:HMM:NF025198.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRT92515.1"
                     /translation="MTLPPRLEIPPAAVTPPSINPKDSPLALTSLITGILGWPILPLL
                     GSIAAIITGHLGKKEIKESHGALTGDGMATTGLILGYIQIGFIVLSLIAVIILLLVLP
                     KSTWGLSL"
     gene            complement(3250..3612)
                     /locus_tag="P5516_08530"
     CDS             complement(3250..3612)
                     /locus_tag="P5516_08530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HRT92516.1"
                     /translation="MTEENNSSVDTSLEAPKPKKALSFFSILSFIAGIGTYVWFFGMV
                     SSKPILTFLLTPFFALIAVITGHKGRHDIRKSLMDMKGKTLANIGLLLGYVIILTGIF
                     IIVLAVMGVVTIAGLLGS"
BASE COUNT          855 a         1005 c         1045 g         1029 t
ORIGIN      
        1 cccaacaaat aacaggggat ggtggcagcg ataacgatga gcagtgttga tttaatgcgt
       61 gtgcgtttta ccgggtcctg tatcatgagg ggttcctgcc cctcatatta tcacgtgcct
      121 tacaactggg caagcgttca tacagtgatg aagcagtcat ttctttagtt cgatattact
      181 gctcgcgttt acctggcgga tttcctgctg gacggtgacg taaaggcttt ccgctttgag
      241 gtcagtgatg gtatagcagg gtgctttaag gtggtctgcc agtgactgcg ctaacccctg
      301 gtcgaaagcg gcgtgttcca tgttgaccac cacactatga atgttttctt tggcaatctg
      361 gtcagcgata tggtaagcct cttcttgcgg tgaactgaag ccgatggaaa cattgcctgc
      421 gccatctgtg agcacgataa gcagaggcat cacctctgga tgcaggattt tttccttgcg
      481 cagcacttcg taagccatca tcaaccccgc gctgagagga gttttaccgc caaccgggat
      541 gtccttgagc gcggcttctg ccagcatcac cgagttggtg ggagaaagca cctgcgtggc
      601 gaggttcttt tgaaaaacga tcaagcccac gcggtcgcgc cgctggtaag cgtctgtcag
      661 cagtgacaga atggcacctt tggtggcggc catgcgctca gccaccgcca ttgaccatga
      721 agcatcaacc agaaaaagga tgagattggc tgctttgcgg acgcgcactt ttttcatgta
      781 atctgccggt ttcacggcaa aggctacgcg gtggcgctct tttatttcag cgcgttcttt
      841 ctggaacggc gctgctgcgc gcacagtggc gtcaaacgcc aggtcatccg tttttccctg
      901 cgctgggcgc gattgcacat aacgcccgcg cttgcgttca gtgcgggtta cggagcgcct
      961 gccggcggat gtgcgcatga ttttatccag cggggtgtca agcttacgag gattgaattc
     1021 ctctccattt ggcatcttct taccggcagc ccaccaaaaa ggatcgttct tgttaaacgg
     1081 gttctgcagg ctcggttctg gttgcccctg ttgcggggag ggtccgccgg tctcttccgg
     1141 cgtcaggctt tttttgggct gcctgtctcc tcgccaccca attcggaaat cgtttcttct
     1201 tcattctcgc cggaagtacc gccctgcagt tgctcaatgc gttcctgcag ttcttccatg
     1261 cccatctctt cgcgttggaa gggcccgctc ttcaggcgat gagggagcgc cagttcggcg
     1321 gcgagagcga tgtcgcggtc gttgatctgc cggcggccgt cgaatgccgc ctgtgcccgt
     1381 gatgttttaa ggatgaccag gtcggaacgg tgaccatcca cattaagaga ggcggttagc
     1441 gctgctatgg aaagcaggtt ctggcgggtg taggttactt catcgaccaa cttgcgtgca
     1501 ttctcgatct ggtgcgacag ctcttgctct ttttcttccc atgaatcgta aaagagtttg
     1561 ggatcttctt cgaaggcgat gtttcgttcc atgatctgaa cccgttcgcg cgcatctcgt
     1621 atgccgcgaa tctcgacaga tagcgcgaag cgatcaagca gttgaggtcg caaatcacct
     1681 tcttcagggt tcatggttcc caccaggata aatcgcgccg ggtgagtgaa ggaaatccct
     1741 tcgcgttcca cgatattcac acccatggcg gccgcgtcta agagcacatc caccacatgg
     1801 tcgtcaagca ggttcacttc atcaatgtag agcagcccac ggttggccgc ggcgagcaca
     1861 cctggttcaa aactgcgcac acctttttgg atggcttttt caatatccag tgtgccaacc
     1921 acccggtctt cagtggctga aaccggcaag ttgacaaacg gcgtgtggcg ggtgatggct
     1981 tcgagttttt cacttctttc agcgcgttcg cggcattcgg tgcaccaggt ggcgggtttt
     2041 tcggggtcgc agccaaaacg gcactccgca actactttga cgctgggtag caatgcggcc
     2101 agcgcgcggg ctgcggttga ttttgccgta cccctttcac cacggataag tactccgcca
     2161 attcgtgggt ccacagcgtt taacacaagc gcacggcgca ttctttcctg tccaacgatt
     2221 gcagtaaatg ggtaaattgc gggcataaag cagttcctta agatggtttt cagatagcgc
     2281 gataagttta ccataatcct ttttcacagg cgtcgttcca tgcatttttc tccgttcccg
     2341 ctctgagtgg atgatcgccc gggaaaaatc aacctcccgg gtgatcaaca ccggtaggtt
     2401 aaagtaggct cgatggctgt gtttggtcag gcgttatttt tttacaggcc aagttctgac
     2461 ctggcgatca gtaaaaaggc gagcacaacg accaggatgg caagcagcga gatgccgatc
     2521 attgtgtagc ccagtatcag gccagcaagg gccatgccgt ctccactcat cgtgtttccg
     2581 ctctcacgga tctctttctt ggcgaggtga cctgtgatca cggcgatgac cgaaccgata
     2641 aaggggatga tcgtccaccc gatgatgccg gccaccaggc tgatgatggc caggctgctt
     2701 tcacgtttga ctatcggaaa gactggaact ccttgttcat aattggaagg gggcgttgtc
     2761 attttattct cctgttgaat tcttttcacc tgttagatat acgaagcttc gggttcaagg
     2821 ttgcaggtgc gatgcaaaat gattcgctct tcacctgtga aaggtggatc agagagacaa
     2881 cccccaggtg ctcttgggaa gaaccagcaa cagaatgatc accgcgatca ggctcagaac
     2941 aataaaaccg atttgaatgt agcccaggat caagccggtg gtcgccatcc cgtcgccggt
     3001 cagcgcgccg tggctttctt tgatttcttt ttttccaagg tgaccggtaa tgatggccgc
     3061 gatggaaccc agcagcggaa ggattggcca tccgaggatg ccagtgatca gactggtaag
     3121 cgcgagcggg ctgtcttttg ggtttatgga tggcggagtt accgccgctg gcggtatttc
     3181 aagtctgggt ggtagagtca tggtaaggca ctccttctaa ttgttacagg gtgatggttc
     3241 tttggatgat caggaaccga gcaggccggc aatggtgacc actcccatta cggccagcac
     3301 gataatgaag atcccagtca ggatgatcac gtacccgagc aataagccga tattggcgag
     3361 agttttcccc ttcatatcca taagactctt gcgaatatcg tgacgccctt tatgcccagt
     3421 gatcacagcg atgagcgcga aaaacggggt caggagaaaa gtcagtatgg gtttggaaga
     3481 gaccatccca aaaaaccata cgtatgtacc aatacccgcg ataaaactca gaatcgagaa
     3541 aaaactgagc gcttttttgg gctttggcgc ctccagtgaa gtatctactg acgaattatt
     3601 ttcttctgtc atatatcctc ctcaatatta acgattatac ccactcaaac gctctttgac
     3661 cattcactga ttacgcgacc ttttttggat attcgcgtcg gaaataccca tcgttcttta
     3721 acaaccgaat gacctgccag cacaagtata ttttccgaat ggcattgttg atcagcactc
     3781 gcatcagctc tgggattgca tacaatcttt ttcttactat ttcgtctatg aagggataat
     3841 catttggatg agtcattttg ttctccttgt aaggtttggt caccttaagg atacttctga
     3901 ctcaccgctt ttgttaaggt tcaacaattt acag
//
LOCUS       DAOTLW010000081         3535 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_22081_length_3535_cov_9.620402, whole genome shotgun sequence.
ACCESSION   DAOTLW010000081 DAOTLW010000000
VERSION     DAOTLW010000081.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3535)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3535
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22081_length_3535_cov_9.620402"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..946)
                     /locus_tag="P5516_08535"
     CDS             complement(<1..946)
                     /locus_tag="P5516_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HRT92517.1"
                     /translation="MLFSPINPMHIPDGFLSVLLSIVLWVISVVLIGIALRKTGQTIG
                     ERQVPLMGVLAAAIFAGQMLNFSVTGGTSGHLLGAAIAAILLGPWPAVLVMTSVVAVQ
                     ALLFQDGGILALGANLFNMAIIGPFVAYALFTLLMKLFKKQTWGLFVAGFVAAWSSIF
                     IASLACALQLALSGTSPANIAVPAMGAIHALIGIGEGLITIGALAFIFAARKDLLIAD
                     ANKPARNIGVVVGGLLLSLVLVVLSPLASSHPDGLEWVAEKHGFIEVAREAFYNIVPD
                     YSMPGISNPALATIAAGILGAVIVFGVAYGIARAEKRPS"
     assembly_gap    1212..1311
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1312..3486
                     /locus_tag="P5516_08540"
     CDS             <1312..3486
                     /locus_tag="P5516_08540"
                     /inference="COORDINATES: protein motif:HMM:NF012343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HRT92518.1"
                     /translation="YTAPPIPEKAVLAEGQVVFTKEDVQAGQEVFLKYGLMDNGTIWG
                     HGAYLGPDFSASYLHNLSVDAQQSLAESSFQKPYDALTLPEQQAVQLQVTDLLKENRY
                     DPESGVLILTAAEAASFQRQIQVWTDYFASTTSSSGLKQDLISHPEENKQLTAFFAWS
                     AWASVAQRPDKPYSYTNNFPYDPSVGNTATADTVLWSALSLIALLGGLGLVLFVVGRW
                     NFLGWRGKQEGELEEPKPIASEIATPAQRATIKYFAVVVLLLLLQVAMGALVAHYRAD
                     PSTFYGLDLSKILPSNLDRSWHLQIAVFWIATSYVAGALFLAPILSKKDPKHQVAGIN
                     ILFGALVLVVAGSLIGEWLGINNKMGNLWFWLGHQGWEYLELGRIWQILLAIGLLLWL
                     FLLVRALKNASQNPDFKQMASLFIITAIGIPIFYVPAMFINSMTNYAVVETWRFWIIH
                     LWVEGFFELFATVMVAIIFFVLGVTSKKTSIRVIYLDAILYLAGGIVGTGHHWYWTGQ
                     SNLPIALSSVFSALEVVPLTLLTLDAWDFIKISQGKWEIVKRHKWTFYFLMAVGFWNF
                     LGAGVFGFLINLPIVSYYEVGTTLTPNHGHTSFMGVFGMLSLALIVFGLRQVLDDQKW
                     KKVDKFVGVGFWGLNIGLLLMSLLSLFPGGVLQLLDVLKNGYWHARSQAYMGQPLVKT
                     IEWIRMGPDLIFVLAGCIPLAIAVLFTLWQVVKHKKSADANH"
BASE COUNT          823 a          876 c          911 g          825 t
ORIGIN      
        1 cggatgggcg cttttcagcg cgggcgatgc cataagcaac accaaagacg atcactgccc
       61 ccagtatacc agccgcaatg gttgccagcg ccggatttga aattccaggc atgctgtaat
      121 cgggaacgat attataaaaa gcttctctgg cgacttcaat aaaaccatgt ttttccgcga
      181 cccattccag gccatctgga tggctggacg ccagcggaga aagcacaacc agcactaatg
      241 atagaagcaa ccccccgacc acgacaccaa tattgcgagc gggtttgttc gcgtcggcta
      301 taagcagatc tttacgggca gcgaaaatga acgccagcgc tccaatggtg atcaatccct
      361 caccaattcc gatgagcgcg tggatggcgc ccatcgcggg aacagcgata ttcgcgggag
      421 aggtgcccga tagcgccagt tgcagcgcgc aggcaagcga agcgataaaa atggacgacc
      481 acgcggctac gaaaccagca acgaacagcc cccaggtttg ttttttgaag agcttcatca
      541 gcaaggtgaa gagcgcgtat gccacgaagg gaccgatgat ggccatgtta aagaggttgg
      601 ctcccagggc caaaatgccg ccatcttgaa aaagcaaggc ttgaacggca acaacggaag
      661 tcattaccag aacagcaggc cagggaccta aaagaatggc ggcgatagct gcgcctaata
      721 aatggcctga agtaccgcca gtgacactga agttcagcat ctgaccggca aaaatcgctg
      781 cagccaatac gcccatcaac ggaacctggc gttcgccgat ggtttggccg gtcttgcgca
      841 gggcaatccc aatcaagacc acagaaatga cccataagac aattgaaagc aggacagaga
      901 ggaatccatc tggaatatgc atggggttta tcggtgaaaa caacattgtg agcctccggt
      961 gaaagaatgt tagaatggag cgtaaggtaa tttcaggaaa attataaatc aataatttgc
     1021 atatgcaaag atttgcatta tacgttttct gtcggaggga caatgcgcgc ctcttcagta
     1081 gaacaagcca ttctggaaac tttatcgaaa gaacattctc acctgacctc tctacaggta
     1141 tttgaagaaa tccgcgggcg cctgccagcg gtaaatcaat ctactgtgta ccgtgcttta
     1201 gaacggctgg tnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1261 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ntatacggct
     1321 ccgcccattc ctgaaaaagc tgtactggca gaaggccagg ttgtttttac caaagaggat
     1381 gtgcaagcag ggcaggaagt ctttttgaaa tatggcttga tggacaacgg cacaatctgg
     1441 gggcacggtg cctacctggg gcctgatttt tcagcttctt acctgcataa tttgagcgtc
     1501 gatgcgcagc aaagcctggc tgaatccagc ttccaaaaac cctatgacgc gttgaccctg
     1561 cctgagcagc aggcggtgca gttacaggta accgacctgc tgaaagagaa ccgctatgac
     1621 cctgaaagcg gcgtattaat tcttaccgcg gcggaagcgg cttctttcca aagacagata
     1681 caggtatgga ctgattactt tgcttcaacc acctcgagct ctgggctgaa gcaagacctg
     1741 atctcccacc cggaagaaaa taaacaactg accgccttct ttgcctggtc tgcctgggcg
     1801 tcggtggcgc aacggcctga taaaccctac tcgtatacca ataacttccc ttatgacccc
     1861 agcgtgggca ataccgccac tgctgacacg gtcctttgga gcgcgttgag tttgatcgcc
     1921 ctgcttggcg gattgggcct ggtgctgttc gttgtcgggc gctggaactt ccttggttgg
     1981 cggggaaagc aagaaggtga actggaagag ccgaagccga ttgcctcaga aatcgccacg
     2041 cctgcccagc gcgccacgat caagtatttt gcggtcgtcg tgcttctatt actgttacag
     2101 gtggctatgg gggctctcgt agcgcactac cgggcagacc cttctacttt ttatggcctt
     2161 gatctttcca agatcctgcc cagcaacctt gaccgttctt ggcatttaca gatcgccgtt
     2221 ttctggattg ccacttcgta tgtcgctggc gctctatttt tagcgccgat tttgagcaag
     2281 aaagatccaa aacaccaggt cgcgggcatc aacattcttt ttggtgccct tgtgctggtg
     2341 gtggctggca gtttgatcgg cgaatggctg ggtatcaata acaagatggg aaacctctgg
     2401 ttctggctgg gacaccaagg ctgggaatac ctggaattag gccgtatctg gcaaattctg
     2461 cttgccattg gtttgctgct gtggcttttc ttgctggtcc gcgcgcttaa gaacgccagc
     2521 caaaacccag atttcaagca gatggcttca ctgttcatca ttacggcgat aggcatcccg
     2581 attttttatg tgccggccat gttcatcaat agcatgacca actacgccgt ggttgaaacc
     2641 tggcggtttt ggatcatcca cctgtgggtg gaaggcttct tcgaactctt cgcaaccgtg
     2701 atggtagcga tcatcttttt tgttctggga gtcacctcaa agaagacttc tatccgcgtg
     2761 atctacctgg acgccatcct ttacctggct ggcggtattg tgggcaccgg tcaccactgg
     2821 tattggaccg gacaatctaa cctgccaatc gcgctgtctt ctgttttctc cgcgctggaa
     2881 gtggttccgc tcacccttct cacccttgac gcctgggact ttatcaaaat ctcccagggg
     2941 aaatgggaaa ttgtcaaacg ccacaagtgg actttctact tcttgatggc tgttggcttc
     3001 tggaacttcc tgggcgcggg agtgttcggc ttcctcatca acttaccgat cgtcagttac
     3061 tatgaagttg gcacaacgct cacgccaaat cacgggcaca cctcttttat gggtgtgttt
     3121 ggcatgttga gcctggcgtt gatcgttttt ggcttgcgcc aggtgctgga tgatcaaaag
     3181 tggaagaaag tcgataagtt cgtcggagtg ggattttggg gcctgaatat cggtttgtta
     3241 ctgatgtcgc ttctctcgtt gttccccggt ggggtgcttc aactgctgga cgtgcttaag
     3301 aacggctact ggcatgcccg cagccaggcg tacatgggtc aaccgctggt aaaaacgatt
     3361 gagtggatca gaatgggacc ggacctgatc tttgtgttag ccgggtgcat tccgctggcg
     3421 atcgctgttc tgttcaccct ctggcaggtg gtaaagcaca agaagtccgc tgatgccaac
     3481 cactaacttc gagtgaactc acctggcagc acctggaaca gggacagaat aattt
//
LOCUS       DAOTLW010000082         3419 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_22958_length_3419_cov_9.574614, whole genome shotgun sequence.
ACCESSION   DAOTLW010000082 DAOTLW010000000
VERSION     DAOTLW010000082.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3419)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3419
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_22958_length_3419_cov_9.574614"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1716
                     /locus_tag="P5516_08545"
     CDS             <1..>1716
                     /locus_tag="P5516_08545"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HRT92519.1"
                     /translation="PGAPNEAYVPTDLAPTVASTIPANGGTLAKDGDIVITFSEPVTV
                     AEGWFNIACTVSGAYTAVVTDADPVYTLNPDADFTGGESCTVTIDHEKVTDDDLIDPP
                     DYMEADYTFTFSVALGCGDTYTPIPAIQGDGMSTPIPGQVVSTEGIVVADYQTNAYVS
                     GTRNGFFMQSVTPDDDPNTSEGLFIYSYLLDVAVGDHVRVTGKATEYNGLTELSPVSQ
                     IQVCGTGNSISPTEFTLPADNLLDFERLEGMLVTIPQELVISEYYNFDRYGEIVLATQ
                     RFMTYTAANEPSVDGFAAWLDNLKLNSITLDDGRESQNPDPAIHPNGLEFTLENRFRG
                     GDLVSDVTGVMDYEFSKYRIQPTQGANYTEANPRPEAPELAPGFLKVASFNVLNYFTT
                     IDLGTGHWICGPSGDMECRGADTAEELTRQRAKILAALSGIDADIFGIMEIENDHPLG
                     EGESPDYAVADLVAGLNAEFGEGTFAYIATGAIGTDAIKQAIIYKPAAVTPVGEFQLL
                     TTAVDPRFIDTLNRPVLAQVFEDNLTGETFVVAVNHLKSKGSACTGDPDLLDGQGNCN
                     LTRKA"
     assembly_gap    1717..1816
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            <1817..3133
                     /locus_tag="P5516_08550"
     CDS             <1817..3133
                     /locus_tag="P5516_08550"
                     /inference="COORDINATES: protein motif:HMM:NF037144.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HRT92520.1"
                     /translation="GDPDLLDGQGNCNLTRKAAAEALVDWLANPTYFPDVENALIIGD
                     LNSYDHEDPIDMIKLGADDMEDTDDDYLDMIAELRGESAYGYVYDGQIGYLDYALANT
                     SLAERIVDVNFWHINADEPDILDYDMTFKQDAQDALYAPDAYRSSDHDPVIVTISFNA
                     APVAEDMEVSTDEDTAVDITLNVSDANEDPLTAEILTEPLHGTYEIDGLVVTYTPEAN
                     YNGPDSFTYKVSDGELDSNTATVSITVTPVNDAPVANDMTETTAEDTPKVITVNASDI
                     DGDTLTAEIMTGPANGSVSVNGLEVTYTPSENWYGEDIFTYRVSDGEANSNIATVTIT
                     VTPVNDAPVAESFEVDLQENGSMTFTLLASDVDGDTLTFSIISEPAHGILECTGVNCT
                     YTPNLHWFGTDSFIFKANDGLLDSNEATVTLNVIPLPRIYLPIIFR"
BASE COUNT          807 a          879 c          873 g          760 t
ORIGIN      
        1 caccaggagc gcctaatgag gcttatgttc ctactgacct tgccccgact gtcgctagca
       61 ccatcccagc caatggcggc acactcgcta aagatggcga tatcgtgatt accttcagcg
      121 aacccgtgac ggtcgccgag ggctggttca atattgcctg cacagtttct ggcgcgtaca
      181 ccgcggtggt gacggatgcc gacccagtct acacgctcaa cccggatgcg gactttaccg
      241 gcggcgagag ctgcaccgta acgattgatc atgaaaaggt taccgatgat gatctgatcg
      301 atccgcccga ttatatggag gcagattaca ccttcacctt cagtgtagcc ctcggttgtg
      361 gcgataccta tacaccgatc cctgctatcc aaggggatgg catgagcacg ccgatccccg
      421 ggcaggttgt ctccaccgaa ggcattgtgg tcgcggatta tcagaccaac gcttacgtct
      481 cgggcacacg caacggcttc tttatgcaga gcgtcacgcc cgatgatgac cccaatacct
      541 cagaaggttt gttcatttat tcttatctac tcgacgtggc cgtgggcgac cacgtgcgtg
      601 taacaggcaa agccaccgaa tataacggtt tgactgagct ttcccctgtt tctcaaatcc
      661 aagtttgcgg tactggcaac agcatcagcc cgacggaatt taccttgccg gcggataacc
      721 tgctcgattt cgaacgcttg gaaggcatgc tggtcaccat tccccaagaa ttggtcattt
      781 cggaatacta taacttcgac cgatatggcg aaattgtgct cgccacacag cgctttatga
      841 cctacaccgc agccaacgag cccagtgtgg atggctttgc tgcttggctg gataatctga
      901 agctcaatag catcaccctg gatgacggcc gcgagagcca gaacccagac cctgccattc
      961 accctaatgg gttggagttc acactggaga accgcttccg tggcggggat ctggtgagcg
     1021 acgtaaccgg cgtaatggat tatgagttca gcaagtatcg cattcagcct acacaggggg
     1081 ctaactacac cgaggctaac ccacgtccgg aagcgcccga gctcgcccct ggattcctga
     1141 aggtggcatc attcaatgtg ctcaattact ttaccaccat cgatttggga acggggcatt
     1201 ggatttgcgg tccctcgggt gatatggaat gccgcggcgc ggataccgct gaagaattaa
     1261 cccgccagcg cgccaaaatt ctggcggcgc tctccggaat cgatgcggat atcttcggca
     1321 ttatggaaat cgaaaatgat catcccctgg gcgaaggtga gagccctgat tacgccgttg
     1381 cggatttggt agcaggcttg aatgcagaat ttggcgaagg cacgtttgcc tatatcgcca
     1441 ctggcgctat cggcacggat gccattaaac aagctattat ctataagccc gccgcggtta
     1501 ctccggtggg cgagtttcag ctgcttacta cagccgtaga cccgcgcttc atcgatacgc
     1561 ttaaccgccc ggttctggcg caggtttttg aggataatct caccggcgag acttttgtgg
     1621 ttgcggtcaa tcaccttaag tccaaaggct cagcctgcac gggcgacccc gacttgctgg
     1681 atgggcaggg caactgtaac ctgacgcgta aagctgnnnn nnnnnnnnnn nnnnnnnnnn
     1741 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1801 nnnnnnnnnn nnnnnnggcg accccgactt gctggatggg cagggcaact gtaacctgac
     1861 gcgtaaagct gcagccgaag ccctggtcga ctggcttgcc aacccgactt acttccctga
     1921 tgtggagaac gccttgatta ttggtgacct gaactcttat gatcacgaag accccatcga
     1981 catgattaag ctcggtgcgg atgacatgga ggatacggat gacgactatc ttgatatgat
     2041 agccgagctc cgtggtgaaa gcgcttacgg ttatgtatat gatggccaaa tcggctatct
     2101 cgattatgca ttagccaaca cctcactggc cgagcgcatt gtggacgtga acttctggca
     2161 tatcaatgcc gatgagccgg atattttgga ttatgatatg accttcaagc aggatgcaca
     2221 ggacgcgctt tacgctccgg atgcctaccg ctcttcggat catgaccccg tgatcgtcac
     2281 catcagcttc aatgctgcgc cggtggcaga agacatggaa gtgagcacgg atgaggatac
     2341 tgcggttgat attacgctga atgtatctga tgccaatgaa gatcccctga cggcagagat
     2401 tctcactgaa ccattgcacg gcacttacga aatcgacggg ttggttgtca cttatacccc
     2461 tgaggctaat tacaacggcc cggatagctt tacctacaag gtcagcgatg gcgaattgga
     2521 ttccaacaca gccacagtca gcatcacggt taccccggtc aatgatgctc cggtggcaaa
     2581 cgatatgacc gaaaccactg ccgaggacac acccaaagtg atcactgtaa acgcctctga
     2641 tatcgatggt gatacgctga ccgctgagat catgacgggc ccagctaatg ggagtgtaag
     2701 tgtgaatggt ttggaagtga cctacacgcc aagtgaaaat tggtatggag aagatatctt
     2761 cacctatcgc gtaagtgatg gcgaagcgaa ctcgaacatt gcgaccgtaa ccatcaccgt
     2821 aactcccgtg aatgacgcac cggtggcgga atcctttgag gtggacttgc aggagaatgg
     2881 ctctatgacg tttaccttgc ttgctagcga cgtggatggg gatacgctga cctttagcat
     2941 aatcagtgag ccagcccacg gcatattgga atgcacaggc gtcaattgta catacacacc
     3001 taatcttcac tggtttggca ccgacagctt catctttaag gctaacgatg ggctgctgga
     3061 ctccaacgaa gcgacggtca cactcaatgt gatcccactg ccgcggatct acctgcccat
     3121 tatctttaga taagagcagc tatacctgag gcgcaaacga ggctgaccgc tcggtcagcc
     3181 tcgacctttt aatccatgtg gaaaattaat cgtcaaattg gtaaactcaa tatatacaca
     3241 aaaaaggagt tttaagatga aactgtctgg aaagaaaatc gcatttttag tagctgaagg
     3301 cgttgaagac ttggaatatc atgtcgtcgc gatgcgcctg caagaagagg gcgccgaagt
     3361 gcttaccgcc ggcttggacc tgaaaccctt gcatggcaaa aacggcttgg tgattaccc
//
LOCUS       DAOTLW010000083         3359 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_23507_length_3359_cov_10.089588, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000083 DAOTLW010000000
VERSION     DAOTLW010000083.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3359)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3359
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23507_length_3359_cov_10.089588"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="P5516_08555"
     CDS             <1..732
                     /locus_tag="P5516_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92521.1"
                     /translation="DLDKSWYFIDRVHRREANFPQEKLFIQAVKIKGDIFRSLRANDK
                     AHGYYDQVFRSNVINYPAVESRSLAAVMLADERKNTDALELIQRSIEEAKQKNFAGIG
                     LKARMYALLLFLEKTTLEEFEHQTDALQQEISRRELLNHDVFESWVPAALAELRGDQA
                     RALAGYCELQQRMAASQNVWMEFLALRKIIGMTKKGDDTGGRARTRVKELLAGIALHA
                     ESPAIKGSFQKFRNKWRRYVNDMST"
     gene            complement(844..>3359)
                     /locus_tag="P5516_08560"
     CDS             complement(844..>3359)
                     /locus_tag="P5516_08560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRT92522.1"
                     /translation="ILLFFAASCGEMPSFDYSPAAAYFNPRQSSDMVNSLHMDEKTLQ
                     TLEFPRVIEKLQNYASFSASAELSRALKPAPNLKEALERQTLTTEARLLLNLKADISV
                     GGAHDIRPLLNLASRGGVLTEAELLSVSGTLMASRDLARHFDKFKERAPHLAEIAAFF
                     PPPPGIIEAISRCISERAEVLDNASAKLSSIRSEIRVSHDRLLSRLERMIGDTRNLPM
                     LQEPIITQRNGRYVIPLRAEFKGRLKSIVHDQSSSGATLFVEPLAIVELNNRWHELQL
                     QERDEVRRILTELSGRVGAEAEAINGIVSALARFDFCLMCAKYAEDLRACEPILRPVS
                     PPRDGSHPGTVIKLHQARHPLLSPESVVPIDIDLDAETFALVITGPNTGGKTVTLKTM
                     GLMVMMAQAGLHIPAQSGSELSFFDNVFADIGDEQSIEQSLSTFSGHITNIVRVLERA
                     RTKTLVLLDELGAGTDPQEGSALARAILLHLVEKRIPCLIATHYPELKTLAHATTGVV
                     NASMEFDLKTLRPTYHLTIGIPGRSNALLIAKRLGLPESILDNARKMIDPSELVSDEL
                     LNEIHHQREVARKARSSADRDRSLANNLRQELTRRLEGIEDERRAVLEEARDEAEKQL
                     AELKSEVQALRKQLRQTRQPQPEIDTMVEKLEVLAQKIDQKPRQKTTSPNLPAPALRA
                     GVKVKLRSLKLEGVITSINEDDAEVMVGNLRVKVKTSDLTPVGETDPGIEKSKTTAPV
                     RLPAPKPAATLFIPTPGMELDLRGMRAEDALDKLDAYLAEAYASGMPFVRIIHGKGTG
                     RLRQVIREALRDSACVSTYEEGGEKEGGEGVTIAHLSED"
BASE COUNT          702 a          872 c         1018 g          767 t
ORIGIN      
        1 gacctggata agagctggta ttttatcgac cgggtgcatc gcagagaagc caatttcccc
       61 caggaaaagc tttttatcca ggcggtaaaa attaaaggag atatattccg ttcgttgagg
      121 gcgaacgaca aagcgcacgg atattatgac caggtctttc gttccaatgt cattaattac
      181 ccggcggttg aaagccgttc gctggcggcg gtcatgctgg cggatgagag aaaaaataca
      241 gatgcgttag aactgataca gcgttccatc gaggaggcaa aacaaaaaaa cttcgccggg
      301 attgggttaa aagcgcgtat gtatgcgctg ctgttattcc ttgaaaagac aacccttgag
      361 gagtttgagc atcaaacgga cgcgttacag caggagatct ctcgtcgcga attgctgaat
      421 cacgacgtct ttgaaagctg ggtacccgcg gctttagcag aattgcgtgg agatcaagcc
      481 cgcgcgctgg cgggttattg tgaactgcag cagcgcatgg ccgcgagcca gaatgtttgg
      541 atggaatttc tggcgttgag gaagatcatt ggcatgacca aaaaagggga tgacacaggt
      601 gggcgcgcgc gaacgagggt gaaagaactg ctggctggca tcgctttgca cgccgagtca
      661 ccggcgatca aaggttcttt tcagaaattt cgcaacaaat ggagacgtta tgtcaacgat
      721 atgtcaacgt aaaggttgta taagtgtaca tacgttagcc gttatcgctg cagtcaaagg
      781 gagactgcgc acatgaggcc ggtagtgtta tagggagaat cactatcggc cttatccatt
      841 cattcaatct tcgcttaaat gtgcgatcgt cacgccttct ccgccttctt tttccccacc
      901 ttcttcatac gtactgacac atgcagaatc ccgtaaggct tcgcggatga cctggcgcag
      961 gcgcccggtg cctttgccgt ggatgatgcg cacaaagggc atgccactgg cgtaagcctc
     1021 cgccaggtag gcatccagtt tgtccagcgc atcttctgcg cgcataccac gcaggtccag
     1081 ctccataccg ggtgtcggta tgaaaagggt cgcagcgggt ttgggagcgg gtaagcgcac
     1141 cggcgcggtg gttttgcttt tttcaatgcc tgggtcggtt tcgcccacgg gggtcaggtc
     1201 gctcgttttg accttcacgc gcaggttccc caccatcact tctgcatcgt cttcgtttat
     1261 ggaggtgatc acgccttcaa gtttcagcga gcgcagtttt actttgactc cagcgcgcag
     1321 cgcgggggca ggcaggttgg gagaggtggt tttttggcgc ggcttttggt cgatcttctg
     1381 cgcgagcact tcgagctttt caaccatcgt atcgatctcg ggttgcggct ggcgtgtctg
     1441 tcgcagttgc ttgcgcagcg cctgcacttc gcttttcagt tcagcaagct gcttttcagc
     1501 ttcatcacgg gcttcttcca gcacggcgcg gcgttcgtcc tcaatccctt ccagtcggcg
     1561 ggtcaactcc tggcgcagat tattggccag cgaacgatca cggtctgctg aagaacgcgc
     1621 cttgcgcgcc acttcgcgct ggtggtgaat ctcgttcagc agttcatctg ataccagttc
     1681 cgagggatcg atcattttcc tggcattgtc gagaatgctc tccggcagcc ccagcctttt
     1741 tgcgatgagc agcgcgttgg agcggccggg aattccgatg gtaaggtggt aggtggggcg
     1801 cagggttttc aggtcaaatt ccatactggc gttcaccacc ccggttgtgg cgtgcgccag
     1861 tgttttcagt tcggggtaat gcgtggcgat caggcaggga atccgttttt ccaccaggtg
     1921 cagcaggatg gcgcgcgcca gggcggagcc ctcctgcggg tcggttccgg cgccgagttc
     1981 atccagcagc accagcgttt tggtacgcgc gcgttcaagg acgcgcacaa tgttggtgat
     2041 atgcccggag aaagtagaga gggattgctc gatggattgc tcatcaccga tatcagcaaa
     2101 cacgttgtca aaaaaactca gttcggagcc cgattgcgcg gggatatgca gccccgcctg
     2161 cgccatcatc accatcaacc ccatcgtttt aagggtaacg gtcttgccgc cggtgtttgg
     2221 accggtgatg accagggcga aggtttccgc gtcgaggtcg atatcaatgg ggaccacgct
     2281 ctcagggctc aagagcggat ggcgggcctg gtggagcttg atgactgtac cagggtgact
     2341 gccatcacgc ggcggggaaa cggggcgcag gatgggttcg caggcgcgta aatcctctgc
     2401 gtatttggcg cacatcaggc agaagtcgaa gcgcgccagg gctgaaacga taccgttgat
     2461 ggcttccgct tcggcgccga cacggcctga aagttcggtc aggatgcgcc gcacctcgtc
     2521 gcgctcctgc agctgcaact cgtgccagcg gttgttcaat tccactatcg ccaggggttc
     2581 cacgaagagg gtcgcgccgg aggaagactg gtcatgcacg atcgatttga gccggccttt
     2641 aaactcagcc cttaagggga tgacgtaacg cccattacgc tgggtgatga taggttcctg
     2701 cagcatggga aggttgcggg tgtcacctat catgcgttcc aaccgcgaaa gcaacctgtc
     2761 gtgcgaaacc ctgatctcgg agcggataga cgagagtttg gcggatgcgt tgtccagcac
     2821 ctcggcgcgt tcagaaatac agcgcgagat ggcttcaatg atccccggcg ggggcggaaa
     2881 gaaagcagca atctccgcca ggtggggggc gcgctcttta aatttgtcaa aatgccgggc
     2941 gaggtcgcgc gaagccatga gcgtgccgct caccgaaagc agctccgctt cagtgagcac
     3001 tcccccgcgg ctggccaggt tgagcaacgg gcggatatcg tgcgcgccac ccacgctgat
     3061 atccgcctta aggttgagca gcaggcgcgc ttcagtggtg agcgtctgtc tttccagggc
     3121 ttctttgagg tttggcgccg gcttcagcgc ccgtgacagc tcagcagaag cgctgaagga
     3181 ggcgtaattc tgcagttttt cgatcacccg tggaaactca agggtctgga gggtcttttc
     3241 gtccatatgc agggagttta ccatatctga agattggcgc ggattgaaat atgccgccgc
     3301 ggggctataa tcaaaagagg gcatttcccc gcagcttgct gcgaaaaata gtaagatta
//
LOCUS       DAOTLW010000084         3337 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_23709_length_3337_cov_10.375686, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000084 DAOTLW010000000
VERSION     DAOTLW010000084.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3337)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23709_length_3337_cov_10.375686"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            92..2494
                     /locus_tag="P5516_08565"
     CDS             92..2494
                     /locus_tag="P5516_08565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HRT92523.1"
                     /translation="MDEKTLQTLEFPRVIEKLQNYASFSASAELSRALKPAPNLKEAL
                     ERQTLTTEARLLLNLKADISVGGAHDIRPLLNLASRGGVLTEAELLSVSGTLMASRDL
                     ARHFDKFKERAPHLAEIAAFFPPPPGIIEAISRCISERAEVLDNASAKLSSIRSEIRV
                     SHDRLLSRLERMIGDTRNLPMLQEPIITQRNGRYVIPLRAEFKGRLKSIVHDQSSSGA
                     TLFVEPLAIVELNNRWHELQLQERDEVRRILTELSGRVGAEAEAINGIVSALARFDFC
                     LMCAKYAEDLRACEPILRPVSPPRDGSHPGTVIKLHQARHPLLSPESVVPIDIDLDAE
                     TFALVITGPNTGGKTVTLKTMGLMVMMAQAGLHIPAQSGSELSFFDNVFADIGDEQSI
                     EQSLSTFSGHITNIVRVLERARTKTLVLLDELGAGTDPQEGSALARAILLHLVEKRIP
                     CLIATHYPELKTLAHATTGVVNASMEFDLKTLRPTYHLTIGIPGRSNALLIAKRLGLP
                     ESILDNARKMIDPSELVSDELLNEIHHQREVARKARSSADRDRSLANNLREELTRRLE
                     GIEDERRAVLEEAHEEAEKQLAELRRELQALRRQLRQTRQPQPEIDTVVEKLEALAQK
                     IDQKSRQKTTSPDLTAPPLRAGVRVKLRSLKLEGVITSINEDDAEVMVGNLRVKLKTS
                     DLAPVDEANPGIEKSKTAAPVPLPAPKPAATLFRPTPGMELDLRGMRAEDALDKLDAY
                     LAEAYASGMPFVRIIHGKGTGRLRQVIREALRDSACVSTYEEGGEKEGGEGVTIAHLS
                     ED"
     gene            complement(2606..>3337)
                     /locus_tag="P5516_08570"
     CDS             complement(2606..>3337)
                     /locus_tag="P5516_08570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92524.1"
                     /translation="DLDKSWYFIDRVHRREANYPQEKLFIQAVKIKGDIFRSLGANDK
                     ARGYYDQVFRSNVVNYPAVESRSLAAVMLAEERKNTDALELIQRSIEEAKQKNLAGIG
                     LKARMYALLLFLEKTTFEEFEHQTGELQQEISRRELLNHDVFERWVPAALVELRGDQD
                     RALAGYCELQQRMAASHNVWMEFLALRKIIGMTKRKDDAGSRARTRVKELLASIALHA
                     ESPAIKGSFQKFRNKWRRYVNDMST"
BASE COUNT          756 a         1007 c          860 g          714 t
ORIGIN      
        1 tcgatgctct tttttgtcta gcctttttgt ctcatatgta tctgaactta ttcatccgcg
       61 ccaatcttca gatatggtaa actccctgca tatggacgaa aagaccctcc agacccttga
      121 gtttccacgg gtgatcgaaa aactgcagaa ttacgcctcc ttcagcgctt ctgctgagct
      181 gtcacgggcg ctgaagccgg cgccaaacct caaagaagcc ctggaaagac agacgctcac
      241 cactgaagcg cgcctgctgc tcaaccttaa ggcggatatc agcgtgggtg gcgcgcacga
      301 tatccgcccg ttgctcaacc tggccagccg cgggggagtg ctcactgaag cggagctgct
      361 ttcggtgagc ggcacgctca tggcttcgcg cgacctcgcc cggcattttg acaaatttaa
      421 agagcgcgcc ccccacctgg cggagattgc tgctttcttt ccgcccccgc cggggatcat
      481 tgaagccatc tcgcgctgta tttctgaacg cgccgaggtg ctggacaacg catccgccaa
      541 actctcgtct atccgctccg agatcagggt ttcgcacgac aggttgcttt cgcggttgga
      601 acgcatgata ggtgacaccc gcaaccttcc catgctgcag gaacctatca tcacccagcg
      661 taatgggcgt tacgtcatcc ccttaagggc tgagtttaaa ggccggctca aatcgatcgt
      721 gcatgaccag tcttcctccg gcgcgaccct cttcgtggaa cccctggcga tagtggaatt
      781 gaacaaccgc tggcacgagt tgcagctgca ggagcgcgac gaggtgcggc gcatcctgac
      841 cgaactttca ggccgtgtcg gcgccgaagc ggaagccatc aacggtatcg tttcagccct
      901 ggcgcgcttc gacttctgcc tgatgtgcgc caaatacgca gaggatttac gcgcctgcga
      961 acccatcctg cgccccgttt ccccgccgcg tgatggcagt caccctggta cagtcatcaa
     1021 gctccaccag gcccgccatc cgctcttgag ccctgagagc gtggtcccca ttgatatcga
     1081 cctcgacgcg gaaaccttcg ccctggtcat caccggtcca aacaccggcg gcaagaccgt
     1141 tacccttaaa acgatggggt tgatggtgat gatggcgcag gcggggctgc atatccccgc
     1201 gcaatcgggc tccgaactga gtttttttga caacgtgttt gctgatatcg gtgatgagca
     1261 atccatcgag caatccctct ctactttctc cgggcatatc accaacattg tgcgcgtcct
     1321 tgaacgcgcg cgtaccaaaa cgctggtgct gctggatgaa ctcggcgccg gaaccgaccc
     1381 gcaggagggc tccgccctgg cgcgcgccat cctgctgcac ctggtggaaa aacggattcc
     1441 ctgcctgatc gccacgcatt accccgaact gaaaacactg gcgcacgcca caaccggggt
     1501 ggtgaacgcc agtatggaat ttgacctgaa aaccctgcgc cccacctacc accttaccat
     1561 cggaattccc ggccgctcca acgcgctgct catcgcaaaa aggctggggc tgccggagag
     1621 cattctcgac aatgccagga aaatgatcga tccctcggaa ctggtatcag atgaactgct
     1681 gaacgagatt caccaccagc gcgaagtggc gcgcaaggcg cgttcttcag cagaccgtga
     1741 tcgttcgctg gccaataatc tgcgcgagga gttgacccgc cgcctggaag ggattgaaga
     1801 cgaacgccgc gccgtgctgg aagaagccca tgaagaagcc gaaaagcagc ttgctgaact
     1861 gagaagagaa ttgcaggcgc tgcgcaggca actgcgacag acacgccagc cgcagcctga
     1921 gatcgatacg gtggttgaaa agctcgaagc gctcgcgcag aagatcgatc aaaagtcgcg
     1981 ccaaaaaacc acctctcccg acctgactgc ccccccgctg cgcgccgggg tcagagtaaa
     2041 actgcgctcg ttgaaacttg aaggcgtgat tacctccata aacgaagacg atgcagaagt
     2101 gatggtggga aacctgcgcg tgaagctcaa aacgagcgac ctggcccccg tggacgaagc
     2161 caacccaggc attgaaaaaa gcaaaaccgc cgcgccggtg cccttgcccg ctcccaaacc
     2221 cgctgcgacc cttttcagac caacgcccgg gatggagctg gacctgcgtg gtatgcgcgc
     2281 agaagatgcg ctggataaac tggatgccta cctggcggag gcttacgcca gcggtatgcc
     2341 ctttgtgcgc attatccacg gtaaaggcac cgggcgcctg cgccaggtca tccgcgaagc
     2401 cttacgggat tcagcatgtg tcagtacgta tgaagaaggt ggggaaaaag aaggcggaga
     2461 aggcgtgacg atcgcccatt taagcgaaga ttgactgaat ggataaggcc gatagtgatt
     2521 ctccctaaaa cactaccggc ctcatgtgcg cagtctccct ttgactgcag cgataatggc
     2581 taatgtttgt acatttatac aacctttacg ttgacatatc gttgacataa cgcctccatt
     2641 tgttacgaaa tttctgaaaa gaacccttga tcgccggtga ctcagcgtgt aaagcgatgc
     2701 tagccagcag ttctttcacc ctcgttcgcg cgcgacttcc tgcgtcatct ttccttttgg
     2761 tcatgccaat gatcttcctc aacgccagaa attccatcca aacattgtgg ctcgcagcca
     2821 tgcgttgctg cagttcacaa taacccgcca gcgcgcggtc ttgatctcca cgcaattcta
     2881 ctaaagccgc cggtacccac ctttcaaaga cgtcatgatt cagcaattcg cgccgagaga
     2941 tctcctgctg taactcgccc gtttgatgct caaattcctc aaaggttgtc ttttccagga
     3001 ataacaacag cgcatacata cgcgctttta acccgatccc ggcgagattt ttttgttttg
     3061 cttcctcgat ggaacgctgt atcagttcca acgcatctgt attttttctc tcctccgcca
     3121 gcatgaccgc cgccagcgaa cggctttcaa ccgccgggta attaacgaca ttggaacgaa
     3181 atacctggtc ataatatccg cgcgctttat cgtttgcccc caatgaacgg aagatatctc
     3241 ctttgatttt gaccgcctgg ataaaaagct tttcctgggg gtaattggct tctctgcgat
     3301 gcacccggtc gataaaatac cagctcttat ccaggtc
//
LOCUS       DAOTLW010000085         3331 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_23771_length_3331_cov_7.708791, whole genome shotgun sequence.
ACCESSION   DAOTLW010000085 DAOTLW010000000
VERSION     DAOTLW010000085.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3331)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3331
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_23771_length_3331_cov_7.708791"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..513
                     /locus_tag="P5516_08575"
     CDS             <1..513
                     /locus_tag="P5516_08575"
                     /inference="COORDINATES: protein motif:HMM:NF024919.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HRT92525.1"
                     /translation="YNLTCEARSAVDWARFFGANISEMEFQAALPLSDNPKLGFVGYY
                     DDPMGRLPPLAYGVYAAPVAQTLAGFGVDAAAVSGYSLNDLRRQVASDNPVIVWVVGN
                     TWWGGTPVDYRAEDGTTVTVVRYEHTVILIGYDAYGVMLVDGGQVYWRSYDTFLASWA
                     VLGNMAVIRQ"
     gene            complement(559..1818)
                     /gene="ahcY"
                     /locus_tag="P5516_08580"
     CDS             complement(559..1818)
                     /gene="ahcY"
                     /locus_tag="P5516_08580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HRT92526.1"
                     /translation="MPNYDVKELNLAEGGRLKIDWAEREMPVLRQVKERFRREQPLKG
                     LRISACLHVTTETANLMKTLQAGGADVVLTASNPLSTQDEVAASLVSHDEIEVYAVKG
                     EDNVTYYKHINAALDHKPHFTMDDGADLVNVLHQHRRELLPGVVAGTEETTTGVIRLH
                     AMAADKALAYPIVAINDALTKHFFDNRYGTGQSTLDGIVRATNVLLAGKTFVVAGYGW
                     CGRGLASRARGMGSNVIVTEVDPLKALEAVMDGFRVMPMADAARIGDIFCTVTGDINV
                     IDAHHFDLMKDGAIVANSGHFNVEINIPALEKSAKSKRLVRPFVEEYVTPDGRRIFIL
                     GEGRLINLASAEGHPASVMDMSFANQALTLEYLAKNASKLEKRVYAVPEEIDKKIAEL
                     KLAAMGVSIDTLTAEQVRYLASWQEGT"
     assembly_gap    1954..2053
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(2199..>3331)
                     /gene="mtnA"
                     /locus_tag="P5516_08585"
     CDS             complement(2199..>3331)
                     /gene="mtnA"
                     /locus_tag="P5516_08585"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF004326.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HRT92527.1"
                     /translation="ILHSPLFHVKLSGKGRSRPRAFFSELRTIEWLPESREVRLIDQR
                     RLPAALEYLTIQTPEQMVEAIRTMAIRGAPALGVAGAFGLALQAARYNRAGGKDLRSL
                     VHAAAVMLVNSRPTAVNLAWGVEQITSLLDEQTHSESELVERIISNAQALAEADVRTN
                     LRLAENGASLIDDGDTIIHHCNTGALAVVDWGTALGAIRFAHEQGKRVHVLVDETRPR
                     LQGSRLTAWECEQYGIPYEIITDNAAGFFLQSGRVHKVFFGADRVAANGDVVNKVGTY
                     MLSLAAHANQVPVVCVFPLSTLDLSIAEGRYIPIEERDAAEVLNLRVDGQAIAPATAR
                     ALNPAFDLTPHALISAWVTEAGVVRAPFAQNLAQSRYNQKMD"
BASE COUNT          647 a          927 c          955 g          702 t
ORIGIN      
        1 tacaacctca cctgtgaagc gcgctccgcg gtggattggg cgcggttttt cggcgcgaac
       61 atcagcgaga tggagttcca ggcagcgctg ccgctttcgg acaacccgaa gctgggcttt
      121 gtgggttatt acgatgaccc gatggggcgc ctgccgcctc tggcttacgg cgtgtacgcc
      181 gcgccggtgg cgcagaccct ggcgggcttc ggggttgacg cggcagcggt gagcggttat
      241 tccctcaatg acctgcgccg ccaggttgcc agcgacaacc ccgtgatcgt gtgggtggtg
      301 ggcaacacct ggtggggcgg cacgccggta gattaccgcg cggaagatgg caccacggta
      361 accgtggtac gctatgagca caccgtcatc ctgattgggt atgacgctta cggcgtgatg
      421 ttggtggatg gcgggcaggt gtactggcgc agctacgaca cctttttggc ttcatgggcg
      481 gtgctgggca acatggcggt catccgccaa taaaaacgga gcctggcggg ctccgttgtc
      541 tcagctgcgg gcggtggttt aggtgccttc ctgccaggag gccaggtagc gaacctgttc
      601 agcggtgagc gtgtcaatgc ttacgcccat ggccgccagt ttaagctccg cgatcttctt
      661 atcaatctcc tcggggacgg catacacgcg cttctccagt tttgaggcgt tcttcgccag
      721 gtattcgagg gtgagcgcct ggttggcgaa agacatatcc atcacagaag ccggatgacc
      781 ttcagctgaa gccaggttga tcaagcgtcc ctcgccgagg ataaagatgc gccgcccgtc
      841 aggggtgacg tattcttcca caaaggggcg caccaggcgc ttgcttttgg cgcttttttc
      901 cagcgcgggg atgttgattt caacgttgaa atgccctgaa ttggccacga tggcgccatc
      961 tttcatcagg tcaaagtggt gcgcgtcgat gacgttgatg tcaccggtga cagtacagaa
     1021 aatatcgcca atccgggcgg catctgccat gggcattacc ctgaaaccgt ccatcactgc
     1081 ctcgagcgcc ttgagcgggt cgacctcggt gacgataacg ttggaaccca tgccgcgcgc
     1141 cctggaagcc aatccgcgtc cgcaccagcc gtagccggca accacgaagg tcttgcctgc
     1201 cagcaacacg ttagtggcgc gcacaatgcc atccagtgtg gattgaccag tgccatagcg
     1261 gttatcaaag aaatgcttgg tgagcgcgtc gttgatagcc acgatggggt acgccagcgc
     1321 tttatccgct gccatagcgt gcaggcggat gacgccggtg gtggtctctt cagtaccggc
     1381 gacgacgccc ggcagcagtt cacggcggtg ctggtgcagc acattgacca ggtcagcgcc
     1441 gtcatccatg gtgaaatgcg gcttatggtc aagcgcggcg ttgatgtgct tgtaataggt
     1501 aacgttgtct tcgcccttta ccgcgtacac ctcgatctca tcatgagaga cgagcgaagc
     1561 cgccacctcg tcctgcgtgg aaagcgggtt agacgcggtg agcaccacgt cagccccacc
     1621 ggcctgcagg gtcttcatca ggttggcggt ctcagtagtc acgtgcaggc aggctgaaat
     1681 acgcagccct ttaaggggct gttcacggcg aaaacgctct ttcacctggc gcagcaccgg
     1741 catttcgcgt tccgcccagt ctatcttcag gcgtccgcct tcggccaggt tcaattcttt
     1801 tacatcatag ttcggcatga ttcctccaaa tggttgtctt taaggttgat ttaaaatatc
     1861 caaagtgccg ccgtcatcaa aaaaggtgcg gtagcgtgtg atatattcct gcttggtgta
     1921 aaaatggttg ctgggtccat tctgttccag gcannnnnnn nnnnnnnnnn nnnnnnnnnn
     1981 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     2041 nnnnnnnnnn nnnccgcgct ggcgggtcat gttatccacc aggaatgaaa ggctgatctt
     2101 atccagttta tccggcagga tgttctcgcg aaaataaccc gggaaagtca tcaggtaatc
     2161 aaaagccaca gaaccgctaa tcacaattgc catattgatt aatccatttt ctgattatat
     2221 ctggattgcg ccaagttttg cgcaaaaggc gcgcggacca caccggcctc ggtaacccag
     2281 gcgctgatga gcgcgtgcgg ggtgaggtca aaggcaggat tgagcgccct ggcagtcgct
     2341 ggggcgatgg cctgtccgtc aacccgcagg ttcagcacct ctgcagcgtc gcgctcctcg
     2401 atcgggatgt acctgccttc agcaatggac agatccagcg tacttaaagg gaaaacgcac
     2461 accaccggca cctggttggc gtgcgccgcc aggctgagca tgtaggtgcc aaccttgttg
     2521 accacatccc cattggctgc cacacggtct gcgccgaaga acaccttgtg aactcgaccg
     2581 ctttgcaaaa agaaccccgc ggcattatca gtgatgatct cgtaggggat gccatactgt
     2641 tcgcattccc aggcggtgag gcgagagccc tgcaggcgcg ggcgggtctc gtctaccagc
     2701 acatgcacac gtttgccttg ctcatgggca aagcgaatgg cgccaagcgc ggtaccccag
     2761 tcaaccacag ccagcgcccc tgtattacag tgatgaatga tggtgtcgcc gtcatcgatc
     2821 aggctggcgc cgttttcagc caggcggagg ttggtgcgca cgtcagcctc agccagcgcc
     2881 tgggcattgc tgatgatacg ctccacaagt tcgctttctg agtgggtttg ctcatccagt
     2941 agagacgtga tctgctccac tccccaggcc aggttcaccg ccgttgggcg ggagtttacc
     3001 agcataaccg cagcggcgtg cacgagcgac cggagatcct ttccacccgc gcggttatac
     3061 ctggctgcct gcagcgccaa tccaaaagcg ccggccacgc ccagggcggg agccccgcgg
     3121 atggccatgg tacgaattgc ttccaccatt tgctctggcg tctggatggt gaggtattct
     3181 aaagctgccg gcagccgacg ttggtcgatc agccgaactt ccctgctctc cggtaaccat
     3241 tcgatcgttc tcaattcact aaaaaacgcc ctcggtcgag agcgtccctt cccactaagt
     3301 ttaacatgga ataaagggga atgcaatatt t
//
LOCUS       DAOTLW010000086         3171 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_25213_length_3171_cov_7.383184, whole genome shotgun sequence.
ACCESSION   DAOTLW010000086 DAOTLW010000000
VERSION     DAOTLW010000086.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3171
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25213_length_3171_cov_7.383184"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            211..294
                     /locus_tag="P5516_08590"
     tRNA            211..294
                     /locus_tag="P5516_08590"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:245..247,aa:Leu,seq:taa)
     gene            430..1809
                     /locus_tag="P5516_08595"
     CDS             430..1809
                     /locus_tag="P5516_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92528.1"
                     /translation="MPKRNSSLLIFLLFSLLMAGCSPEATFPTALATLPQPTAVNTPL
                     PGEPTWTPSPQPINPTPTVNVAEPNPVQITELAHVQVQRELVVAIKFQNILPDALLRD
                     VNYEILALDATGNRIAQEAGVIPFLIPEQTMGLVRSFQLIAGVQAETVEVRFSAGTQD
                     RDLKLQSPFEVINPAFFVDLKGAVMTGWLQNRDKQTYTEVEVNAIGYNAKGEIVGGGS
                     ILTEFVPEKDRIGVSIPVTVSEAPVLVEIYPWISTYSASLEGGSWWKNIKVQDWDFII
                     TDQGYVTGGAILTNITDQVLTGTYYIITVADAQDRVCAVSRGFIPMLLPGETLNFSPG
                     ILYPPTTAQAAKVDLIIVPGEFGEYPLAYNPLSITQSTLLLEEQEVPIVRTTVLNNLN
                     ASLSGTLVTVVLKNERGRIVGGGQALTGQILAGSTLQVDVPVGFIGNPENLTIAASVT
                     LPTGVVIGE"
     gene            1836..>3171
                     /locus_tag="P5516_08600"
     CDS             1836..>3171
                     /locus_tag="P5516_08600"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HRT92529.1"
                     /translation="MTSFKQKPGLRRFIPTPINPQNPVPPDIEIAQAATLKPIAQVAE
                     EAGLLPEEIELYGDYKAKIKLSVLERLADVPDGKYIDVTAITPTPLGEGKTTTTVGLS
                     QALGAHLGQRVFTCIRQPSQGPTFGIKGGAAGGGYSQVIPMEDFNLHLTGDIHAITAA
                     NNLLAAAIDTRMFHEAQQSDSALFNRLCPPDKHGQRHFAIGMLGRLQKLGITESDPEK
                     LSPEERARFARLDIDPETVTWRRSVDVNDRMLRGITVGQGPEEKGFTLKTNFYISVAS
                     ELMAILALSTSLADMRARIGRVVIGLNRQGEPITTEDLGVAGALTVLMKDTIKPNLMQ
                     TLEGTPVLVHAGPFANIAHGNSSIIADMIALKLADYVVTESGFGSDMGMEKFFDIKCR
                     YSGNIPNAVVLVATVRALKMHGGGPKVTAGTPLAPEYTEENLDLVRAGAENLL"
BASE COUNT          768 a          920 c          797 g          686 t
ORIGIN      
        1 caacacagcg gtaacccagc cggctgcgct cgcgcgtttg ccgctagccc tcaagcctaa
       61 catcaagacc atcacaacca gcaaaggcaa gagggaaagc aaccaggtga acaggtttag
      121 cggcataaca aaattatagc cgcagcgctt gcaacctgaa atattttgtt gcaaaccttg
      181 cattcatggt aaaataagtg cacttcacac ggggcggtgg cggaataggc agacgcaacg
      241 gacttaaaat ccgtcggtgg agacaccgtg tgggttcaag tcccacccgc cctatgtaga
      301 gtttttaagg ctatataagc ccgcccgcta aaaacggtat aatctacctg cttatgctta
      361 ccatattcgc ccctaaaatc agcacccatc caatctgcct gctttatacg tctttttgga
      421 aggtactgaa tgcctaaacg caacagttcg ttgcttatct ttctcctttt cagcctcctc
      481 atggctggct gcagcccaga ggcaaccttt ccaactgcct tagccaccct accccagccg
      541 acggcagtca atacccccct gcctggcgaa cccacctgga cgccctctcc tcagcccatc
      601 aatcctacgc cgactgtgaa cgtggcggag cccaacccgg tgcagattac cgagttagcc
      661 cacgtgcagg tgcagcgcga actcgtggtt gcaatcaagt tccagaatat cctcccagat
      721 gcccttttgc gcgatgtgaa ctacgaaatt ttggcgttag atgcaaccgg caaccgcatc
      781 gcccaagaag ccggcgtgat ccctttcctc atccctgagc aaaccatggg tttagtgcgt
      841 agttttcagc ttatcgctgg cgtgcaagct gaaaccgtcg aggtgcgctt tagcgccggc
      901 acgcaagacc gcgacctgaa acttcagtca cccttcgagg ttatcaaccc cgctttcttt
      961 gtggacctga aaggcgcggt gatgaccggc tggctgcaaa accgggataa gcaaacctac
     1021 acagaggtgg aagtcaatgc gattggttac aacgccaaag gtgaaatcgt tggcggtggc
     1081 agcatcctca ccgaatttgt gccagagaaa gaccgcatcg gtgtgagcat ccccgtaacc
     1141 gtctccgaag cgcctgtgct cgtggaaatt tacccatgga tctccaccta ctcagcttct
     1201 ctggaaggcg ggagttggtg gaaaaatatc aaggtccaag attgggattt cattatcacc
     1261 gatcaagggt atgtcaccgg tggggctatt ctcacgaaca tcactgatca ggtacttact
     1321 ggcacgtatt acattatcac cgtcgcggat gcgcaagacc gtgtgtgtgc tgtttcccgc
     1381 ggctttattc ccatgctgct cccaggcgaa acgctcaact tctctccggg gatcctctac
     1441 ccgcccacca cagctcaggc agctaaagta gatttgatta tcgtgccggg agaatttggc
     1501 gagtacccct tagcttataa cccgctctcg attacccaaa gcacgctgct gctggaggag
     1561 caagaggttc ccattgtccg tactacggtg ctcaacaatc tcaatgcgag cctttccggc
     1621 actctggtaa ctgtggtgct taaaaatgag cgcgggcgca tagttggcgg tgggcaagcg
     1681 ctcaccggtc aaatcctcgc gggaagtaca cttcaggtgg atgtgcctgt aggattcatc
     1741 ggtaaccctg aaaacctaac cattgccgca tcggtgactt tgcctaccgg tgttgtgatt
     1801 ggagaataaa tcaatacaga aaaataagga gaactgtgac ttcattcaaa caaaaacctg
     1861 gattgcgtcg ttttatcccc acgccgatta acccccaaaa ccccgtcccg ccagatatcg
     1921 agattgccca ggcagccacg cttaaaccca ttgcccaagt agccgaggaa gccggcttgc
     1981 tgccagaaga aatcgaacta tatggcgatt acaaagccaa aatcaagctg agcgtcctcg
     2041 aacgcttggc agatgtgcca gatggtaagt acattgatgt tacggcgatc acccccacgc
     2101 cgctgggcga aggtaaaacc acgacgaccg tcggccttag ccaagcctta ggggcacact
     2161 taggccagcg cgttttcact tgcatccgtc agccttcgca aggaccaact tttggcatta
     2221 aaggcggcgc agccggcggc ggatattcgc aagttatccc catggaagac tttaatctgc
     2281 acttgacagg cgatattcac gccattaccg cagccaacaa cttgctcgct gctgccattg
     2341 atacacgcat gttccacgaa gcgcagcaga gcgacagcgc cctgttcaat cgcctttgcc
     2401 cgccagataa acacggtcaa cggcattttg ccatcggcat gcttggtcgc ctgcagaagc
     2461 taggcattac cgagagcgac ccagaaaagc tcagcccgga agaacgcgcc cgcttcgccc
     2521 ggttggatat tgaccccgaa accgtcacgt ggcggcgcag tgtggacgtt aatgaccgca
     2581 tgctgcgggg cattaccgtt ggtcaaggtc ctgaggagaa gggcttcacc ctcaagacga
     2641 atttctatat ctctgtagcc agtgaactga tggctattct ggctctctcc accagtttgg
     2701 cggatatgcg cgcgcgcatc gggcgcgtcg tgatcggcct caaccgacaa ggcgaaccca
     2761 tcaccaccga agacttaggc gtggctggtg cgctcactgt tttgatgaaa gacaccatca
     2821 agcccaacct gatgcaaacg ctggaaggca caccagtgct cgtgcatgcc ggcccatttg
     2881 cgaatattgc ccatggcaac agttccatca tcgccgatat gattgctctg aagttagcag
     2941 attacgtggt gacggaatct ggcttcggct ccgatatggg catggaaaaa ttcttcgata
     3001 tcaaatgccg ctattccggt aacatcccca acgcagtggt gctcgtcgca acggtgcgtg
     3061 cgctcaagat gcacggcggc gggccaaagg ttaccgctgg caccccgctc gcgcctgaat
     3121 acaccgaaga aaacttggac ctggtacgcg caggcgctga gaacctcctg g
//
LOCUS       DAOTLW010000087         3112 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_25769_length_3112_cov_7.926071, whole genome shotgun sequence.
ACCESSION   DAOTLW010000087 DAOTLW010000000
VERSION     DAOTLW010000087.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_25769_length_3112_cov_7.926071"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..978
                     /locus_tag="P5516_08605"
     CDS             <1..978
                     /locus_tag="P5516_08605"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HRT92530.1"
                     /translation="PAVTFNRLCASGLTAVNAAFRAIRAGEGEIFIAGGVENMSRAPY
                     VLAKAESGYPFGNLTAYDTALGWRFPNPRMAELYGTEPMGETAENIADLYHISREEQD
                     RFAAQSHARAVAAMEDGRMAEEILPVTVTPKKGEPILCDRDERPRRDTTVESLAKLKP
                     AFRQGGSVTAGNSSGMNDGAAALLLMSAEKAAEMGLKPMVKIKASAAAGVEPRVMGLG
                     PVGAARKALQRAGLKPSDLGLVELNEAFAVQALAVLHDLGISETITNVNGGAIALGHP
                     LGCSGARILTTLLHEMKRRAPSAPRPYYGMAMLCVGVGQGEATIVEWAA"
     gene            1174..1416
                     /locus_tag="P5516_08610"
     CDS             1174..1416
                     /locus_tag="P5516_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009120596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HRT92531.1"
                     /translation="MPPKGRIVVSEKYCKGCELCVNACPQSVLGLDHEHLTAKGYHPA
                     VLIAEGCTGCGICAVVCPDAAITVYRIRTGATRELD"
     gene            1427..2539
                     /gene="vorB"
                     /locus_tag="P5516_08615"
     CDS             1427..2539
                     /gene="vorB"
                     /locus_tag="P5516_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     VorB"
                     /protein_id="HRT92532.1"
                     /translation="MAKELMKGNEAVAEAAIRAGVTAYFGYPITPQTELLEYMSRRMP
                     ELGRVFVQAESELAAINMVYGAACTGRRVMSSSSSPGVSLMMEGVSYIAGTELPVVLV
                     DVMRGGPGLGNIAPGQGDYNQIVHGGGHGEYRAIVLAPGSVQESVDLTVLAFDLAEKY
                     RSIVVVLSDGSIGQMMEPCELPPMQPLKTKFPDWAVTGSEGRDRRFLSSINLDPAAQE
                     RMNFRVMRRWQEIEANEVRYKEYYMEDARFVVIGFGSTGRVALSAVRAARAEGIPVGL
                     IRPVTLNPFPTKAIAQAAQQAEVMLVVEMNNGQMLEDVMLAANGNAMVEFYGRMGGVF
                     MLPEEVLGEIRRIAAGGLSTEGHPRDRWLNRSAQKK"
     gene            2550..>3112
                     /locus_tag="P5516_08620"
     CDS             2550..>3112
                     /locus_tag="P5516_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HRT92533.1"
                     /translation="MEMNSQVIEEQVYNRPDSFVDVSTHYCPGCTHGTAHRLIAEVID
                     EMGIKGKTIGVASVGCSVFAYNYFDLDFVQAPHGRAPAMATGVKRVLPERVVFTYQGD
                     GDLASIGTAEIVHAAARGENISVFFLNNTNYGMTGGQMAPTTLPGQKTTSSPRGRDVE
                     TQGYPVKASEMISTLDGASYVVRRSLH"
BASE COUNT          698 a          765 c         1004 g          645 t
ORIGIN      
        1 ccagcggtga ctttcaatcg cttgtgtgct tcgggtttga ccgcggtgaa cgctgctttc
       61 cgtgctatcc gggcgggaga aggcgaaatt ttcatcgccg gcggggttga gaatatgtca
      121 cgcgcgccct atgtgctggc caaggcggaa tcaggctacc ccttcggcaa cctcactgct
      181 tacgataccg cgttgggctg gcgattcccc aatccgcgaa tggcggagct gtatggaacg
      241 gaaccaatgg gggaaaccgc ggaaaatatc gccgacctgt accacatcag ccgtgaagag
      301 caagacaggt tcgcggcgca aagccacgcg cgcgcggtcg ccgcgatgga agacggcaga
      361 atggcggagg agatactgcc ggtgacggtg acccccaaaa aaggtgaacc cattctatgc
      421 gaccgggatg aacgccccag acgggatacc actgttgaga gcctggccaa actgaagccg
      481 gcgtttcgac agggagggtc ggtgacagcg gggaattcat ctggcatgaa cgatggcgcc
      541 gcggcgctgc tgttgatgag cgctgaaaag gctgcggaaa tggggctcaa gccgatggtg
      601 aaaatcaaag cttcagccgc ggcaggagtg gaaccgagag tgatggggtt ggggccagta
      661 ggcgccgcgc gcaaagcgct gcagcgcgcg ggattaaagc catctgacct gggtcttgtc
      721 gaattaaatg aagcctttgc cgtgcaggcg ctggcggtat tgcatgacct ggggatctct
      781 gaaaccatca cgaatgtgaa tggcggggcg attgccctgg gtcacccgct tggctgctcc
      841 ggcgcgcgca tcctcaccac gctgctgcat gaaatgaaac ggcgcgctcc ttctgcgccg
      901 aggccgtatt acgggatggc tatgctatgt gttggggtgg gacagggtga agccaccatt
      961 gtggagtggg cggcatgagt agcatatatg ccaggtcttt attcaagaga gaaagaatcc
     1021 ggttgtcggg caggagtaga actcctgctt ttttaactga aatggacagg aggagagaca
     1081 ctgttcaaaa accgtggttt tgtggataat attaatccac cggtgagaaa accggtgacc
     1141 tatccccaag atggggaatt aaaggaggta taaatgcccc caaagggtcg gattgtcgtc
     1201 agcgaaaaat actgcaaagg ctgtgaactt tgtgtgaatg cctgtccgca aagcgtgctt
     1261 ggattagacc acgaacatct cactgccaaa gggtaccacc ccgctgtgtt gattgcagag
     1321 ggctgcaccg gctgcggtat ttgcgcggtg gtgtgccctg atgcagccat aacagtctat
     1381 cgaattcgta ccggtgcaac ccgggaactg gattaggagg cgggcaatgg caaaagagtt
     1441 aatgaaaggc aatgaagctg tcgccgaggc tgcgatccgc gccggggtga ccgcgtattt
     1501 tggctacccg attacgccgc agaccgaatt actggagtat atgtcaaggc gcatgccgga
     1561 actggggcgg gtatttgtgc aggcggaaag tgagcttgcc gccattaaca tggtctatgg
     1621 cgcggcctgc acagggcggc gcgtgatgag ttcttcatcc agccccggcg tgagcctgat
     1681 gatggaggga gtttcttaca tcgccggcac cgagctgccg gtggtgctgg tggatgtgat
     1741 gcgcggtggg cctgggcttg gcaacatcgc ccccgggcag ggtgattata accagatcgt
     1801 gcatgggggt ggtcatggtg aataccgcgc cattgtgttg gctcccggca gtgtacagga
     1861 atctgtagac ctcacggtgc tggcttttga cctggcggaa aaataccgct cgatcgtggt
     1921 ggtgctttca gacggttcaa ttgggcagat gatggaaccc tgcgaactgc cacccatgca
     1981 gccacttaaa accaaattcc ctgattgggc tgtaactggc tctgaagggc gcgacaggcg
     2041 ctttttgagt tcgattaatc tcgacccggc ggcgcaggaa aggatgaact tcagggttat
     2101 gcgccgctgg caggaaatcg aagccaacga agtacgctac aaagaatact atatggaaga
     2161 cgccaggttc gtagtcatcg ggtttggttc aaccgggcgg gtggcgcttt cagccgtcag
     2221 ggcagcgcgc gccgaaggaa tcccggtggg actgattcga ccagtgaccc tcaatccctt
     2281 ccccacaaaa gccattgccc aggccgcgca gcaggcggag gtgatgttgg tggtggaaat
     2341 gaacaacggg cagatgctcg aggatgtgat gctggcagcg aatggcaacg ccatggtgga
     2401 attctatgga cgcatgggtg gtgttttcat gctaccagag gaagtgctgg gtgagatacg
     2461 acgcatcgcg gctggcgggc tttccactga ggggcatccg cgcgatcgct ggttgaaccg
     2521 cagcgcgcag aagaagtgag gagttgaaca tggaaatgaa tagtcaggtg atagaagagc
     2581 aggtttacaa ccgaccagat tcttttgtgg atgtgagcac gcattattgt cccggctgca
     2641 cgcatggtac agcgcaccgc ttgatcgccg aagtgattga tgaaatggga ataaagggga
     2701 aaaccattgg tgtggcatcg gttggctgtt cggtttttgc ctataactac tttgatttgg
     2761 attttgtcca ggcgccgcat gggcgcgcgc cagcaatggc aaccggggtg aaacgcgttt
     2821 taccagagcg cgtggtgttc acctaccagg gagatggcga cctggcctcc attggtacgg
     2881 cggagatcgt gcacgccgct gcccgtgggg agaatatctc cgtgttcttt ctgaacaaca
     2941 ccaattatgg catgactgga ggtcaaatgg cgcccaccac ccttccgggt caaaaaacga
     3001 cctcttctcc acgcgggcgc gatgtggaaa cacagggcta cccagtcaaa gcatctgaaa
     3061 tgatctccac gctggatggg gcttcttacg tggtgcggcg ttcgctgcac ga
//
LOCUS       DAOTLW010000088         3075 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_26168_length_3075_cov_13.020861, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000088 DAOTLW010000000
VERSION     DAOTLW010000088.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_26168_length_3075_cov_13.020861"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..662)
                     /locus_tag="P5516_08625"
     CDS             complement(<1..662)
                     /locus_tag="P5516_08625"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRT92534.1"
                     /translation="MEWFQLLQRFFGDFMQGAGVTLELTAVGLLIGFVLGLALALAKT
                     FGAPGVSKFATGYIELFRGTPLLVQLFLIYYGLPSLGLTLSQTLSAYLALGLNSAAYQ
                     AEYLRGAIQAIGDAQMMAGRSIGLSRWQTIRLVILPQALRLALPAWSNEPISLLKTTA
                     VVFLIAVQDLMAKAKRAATTTYNPIGSYIAVAVIYLVMVFILDALLKWLERKTKIPGL
                     ET"
     gene            complement(664..1416)
                     /locus_tag="P5516_08630"
     CDS             complement(664..1416)
                     /locus_tag="P5516_08630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002183294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HRT92535.1"
                     /translation="MLTVKNLTKRYGEHTVFENINLTVNEGDTIVIIGPSGTGKSTFL
                     RCLNLLTKPNSGQIWLDDQEITAKDVDEDQVRQHIGMVFQNFLLFNHLTALENVKLGL
                     IHVKHMSKEAAEAKAREELARVGLAEQADHYPGQLSGGQQQRVGIARALAMDPRIMLF
                     DEPTSALDPELIGEVLEVMRKLSIEGMTMIVVTHEMGFAREVADRIVFMEKGQIVEEG
                     TPDQLFYHPQHERTREFLWKITELYGKKEKEA"
     gene            complement(1448..2092)
                     /locus_tag="P5516_08635"
     CDS             complement(1448..2092)
                     /locus_tag="P5516_08635"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HRT92536.1"
                     /translation="MDIGRFFQYIASGTAYTLGVTLIALPSGLILGMLFAFLAVYAGK
                     IATRFFSIYSTIMRGIPPIVLLFILYFLMSGAINLTPFWAGSVSLGIISSAYQMEIFR
                     GAILSVSADQMMAARAIGLSKIQAIRTVIFPQMIRIAIPGWSNEAAIVIKDSSLVYAI
                     GVPEILRRAQLISASTQQPFLAYTTAAFIYFTLVFITNRLLNRAEQKLTIPSNP"
     gene            complement(2171..2965)
                     /locus_tag="P5516_08640"
     CDS             complement(2171..2965)
                     /locus_tag="P5516_08640"
                     /inference="COORDINATES: protein motif:HMM:NF012707.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HRT92537.1"
                     /translation="MKKTLVALSVLLIMAGLLAGCGEQAPTSTLDKIKQAGKVVVGTS
                     ADYPPYEYVDETGNYTGFDIELMTEITKRMGVSLEWVDMPFDSLIAAVQEGKIDMSIS
                     CFNYSEERDQQVDFSDPYYTSQDAFLVAEGFTGTITDPAEIVQYKIGVQSGTVQDDWV
                     TTELIETGLMPESKLSRYERVDQAALDLQAGRIDVLVADSVPAQALIKQFGGFKIVYE
                     VQLYTGPINIVLPEGDKALRDEVNKIIKQLQDEGFIDQLAVKYFSE"
BASE COUNT          758 a          794 c          800 g          723 t
ORIGIN      
        1 tccgtttcca gcccagggat tttggttttc cgctctaacc atttgagcag ggcatcgagg
       61 ataaagacca tgaccagata aatgacagcc accgcgatgt aactgccgat agggttatag
      121 gtggtggtag ctgcccgctt ggctttagcc atcaggtctt gcacagcgat caggaacacg
      181 actgctgtgg ttttgaggag cgagatgggc tcgttggacc aagccggcaa agccagccgc
      241 aaggcttggg ggaggatcac caaccgaatt gtctgccagc gcgaaagccc aatggagcgc
      301 cccgccatca tctgcgcatc cccgatcgcc tggatcgcgc cgcgcaggta ctccgcctgg
      361 tacgcagcgc tgttcaaccc gagcgctaag taagcagaga gcgtttgcga aagggtcaag
      421 cccagagaag gcaagccgta gtaaatcagg aagagctgca cgagcaaggg cgtcccacgg
      481 aagagctcga tataacctgt ggcaaatttg ctcacgcctg gtgcgccgaa agttttggct
      541 aaggctaaag ccaaccccaa cacaaagcct atcagcaagc caacggctgt gagctccaag
      601 gtgacgccag cgccttgcat aaagtcgcca aagaagcgtt gcagcagctg gaaccattcc
      661 accttaagcc tctttttctt tcttaccgta gagctcggtg attttccaca agaactcccg
      721 tgtgcgttcg tgttggggat ggtaaaagag ctgatctggt gtgccttcct cgacgatttg
      781 ccccttttcc atgaacacaa tccggtccgc aacttcgcgc gcaaagccca tttcgtgggt
      841 cacaacaatc atggtcatgc cctcgatgga aagtttgcgc atcacctcaa gcacctcacc
      901 aatcagctct gggtcaagcg cggaagtagg ctcatcgaag agcataatgc gtggatccat
      961 ggccagcgct ctggcaatgc cgacgcgctg ctgctggcca ccggagagct gccctggata
     1021 atggtcagct tgctcggcca agcctacccg agccagttct tcgcgcgctt tggcttcagc
     1081 tgcctctttg gacatatgct tgacatgaat caggcctaat ttaacatttt ccaacgcggt
     1141 cagatgattg aaaagtaaaa agttttggaa aaccataccg atatgctgcc gcacctgatc
     1201 ttcatcaaca tcttttgccg tgatttcctg gtcatccagc cagatctgcc cgctgttggg
     1261 tttagtcagc agattcaggc aacgcaggaa agtgcttttc cccgtgccac tgggcccaat
     1321 aatcacgatg gtatcgcctt cattgaccgt gaggttgatg ttctcgaaaa cggtgtgctc
     1381 cccatagcgc ttggtgaggt tttttacggt gagaatgggt ttagtcatgc ggtgtggctc
     1441 caaaggctca gggattgctg ggaatagtca atttttgctc cgcccggttg agcaaacggt
     1501 tggtgatgaa caccagcgta aaataaatga aagcagcagt cgtgtatgct aagaacggct
     1561 gctgtgtgct ggcgctgatg agctgagcgc ggcgcaaaat ttcgggcacg ccaatggcat
     1621 agaccaatga agagtcctta atcacgatgg cagcctcatt cgaccagcca gggattgcga
     1681 tgcgaatcat ctggggaaaa attacagtgc gaatcgcctg gatcttgctc aagccaatcg
     1741 cccgagctgc catcatttga tcagcgctga cagagagaat ggctccacga aagatttcca
     1801 tttggtaagc actgctgata atgccgaggg agactgaacc agcccagaaa ggcgtcaggt
     1861 tgatggcgcc ggacattaaa aagtagagga taaatagcag cacaatcggc gggatgccgc
     1921 gcatgatggt gctgtagatg ctaaaaaagc gagtagcgat ttttccggca taaaccgcca
     1981 gaaaggcgaa gagcatcccc aaaatcagcc cggatggcag cgcaatcagc gtgacaccta
     2041 aggtatatgc agttcctgag gcgatatact gaaaaaatct gcctatatcc aacggctgtt
     2101 gctctcctgt ttaaaatata gaagcaaatg gccgcttcaa acctctagcg gccattccta
     2161 tctttctcat ttactcgcta aaatacttga cagccagctg atcaataaag ccttcatcct
     2221 gtagctgttt gatgattttg ttgacttcat cgcgtagggc tttatcccct tcgggaagga
     2281 caatgttgat cgggccggta tagagctgga cctcatagac gatcttgaag ccgccaaact
     2341 gcttgatcag cgcctgggct ggcacggaat cggccaccaa aacgtcaatg cggccggctt
     2401 gcaagtccag ggcagcctgg tccacgcgct cataacgcga aagcttgctt tcgggcatta
     2461 agccagtttc aattagctct gtggtcaccc aatcatcctg cactgtgcca gattgcacgc
     2521 caatcttata ttggactatt tcggcggggt cggtaatggt gccggtaaag ccctcagcaa
     2581 ccaagaaggc atcctgcgag gtgtaatacg ggtcagaaaa atcgacctgc tgatcgcgct
     2641 cctcggaata gttgaagcag gagatggaca tatcgatttt gccttcctgc accgctgcga
     2701 tgaggctgtc gaacggcata tcgacccatt ctaagctgac gcccatgcgt ttggtaatct
     2761 cggtcatgag ctcgatatcg aagcctgtgt agttgcccgt ttcatccaca tactcgtaag
     2821 gtgggtaatc ggcagatgtg cctacgacga ccttgcctgc ttgcttaatt ttatccaggg
     2881 ttgaagtagg cgcttgctcg ccgcagccag ccagcaagcc agccataatg agaagaacgg
     2941 acaacgcaac caaagttttc ttcatgtgaa tcctcctcgt ttttaaagga acagtatata
     3001 acgactgctt tcattataaa tggaatttgt ctaaaatctg atgttctttc cttgtttagg
     3061 ttttcattac gggct
//
LOCUS       DAOTLW010000089         2884 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_28224_length_2884_cov_8.553906, whole genome shotgun sequence.
ACCESSION   DAOTLW010000089 DAOTLW010000000
VERSION     DAOTLW010000089.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2884)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2884
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28224_length_2884_cov_8.553906"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1061
                     /locus_tag="P5516_08645"
     CDS             <1..>1061
                     /locus_tag="P5516_08645"
                     /inference="COORDINATES: protein motif:HMM:NF023428.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT92538.1"
                     /translation="IGEGKLLRRAIIADRLFSSILFTGPPGTGKTTLARLIAQHTQAH
                     FENISAVLAGVADLRRVITEATDRRRLYHRRTILFVDEVHRWNKAQQDALLPHVENGM
                     VTLIGATTENPYFDVIPALVSRSRIFQLEPLTEEQLAVIFKRALADEEFGYGKRQVLF
                     DEKAREHLLHQAGGDARNLLNAIELAVETTPAGDDGAIYITLDVAQESIQKRTLLYDR
                     MGDEHYDTISAFIKSVRGSDPDAALYWLAKMIFAGEDPRFILRRLIVLSGEDIGLADP
                     QALQVVAAAAYAYEYIGMPEGIYPIVEATLYLATAPKSNSTGAYFAARAEIEQNGAGP
                     VPVHLMDSNRDAKGFGHGKG"
     assembly_gap    1062..1161
                     /estimated_length=100
                     /gap_type="within scaffold"
                     /linkage_evidence="paired-ends"
     gene            complement(<1162..1484)
                     /locus_tag="P5516_08650"
     CDS             complement(<1162..1484)
                     /locus_tag="P5516_08650"
                     /inference="COORDINATES: protein motif:HMM:NF013854.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HRT92539.1"
                     /translation="MQLLLATTNKGKIGELSALLAGLPLQLLTPQEVSPDTRVEETGR
                     TYLENALLKAFAYQAATGLAVLADDTGLEVEALEGAPGLHSARFSPLPGATDADRRAL
                     LLAHLA"
     gene            1597..2076
                     /locus_tag="P5516_08655"
     CDS             1597..2076
                     /locus_tag="P5516_08655"
                     /inference="COORDINATES: protein motif:HMM:NF014657.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HRT92540.1"
                     /translation="MANQRNPLKINVGFFNNLPIGSFRDIHFESPDLHLPPDLDVTNF
                     SGSVHISRTPQGLLFDGAFRAFIQAECVRCLEPYDQLLETEFSEVYAYKSHSFTESNL
                     YVPDDGNIDLSPVIREYLMLENPIKPLCKPDCQGLCVVCGENLNIATCEHQARIKIE"
     gene            2226..2459
                     /locus_tag="P5516_08660"
                     /pseudo
     CDS             2226..2459
                     /locus_tag="P5516_08660"
                     /inference="COORDINATES: protein motif:HMM:NF015912.2"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma"
     gene            2550..2884
                     /locus_tag="P5516_08665"
                     /pseudo
     CDS             2550..2884
                     /locus_tag="P5516_08665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
BASE COUNT          666 a          750 c          746 g          622 t
ORIGIN      
        1 atcggcgaag gcaaattgct gcgccgcgcc atcatcgccg acaggctctt ttcgtccatc
       61 ctcttcaccg gccctcccgg aactggaaaa accaccctgg cgcgtttgat cgcccagcat
      121 acgcaggcgc attttgaaaa tatctccgct gtactggcgg gtgtggcaga cctgcgcagg
      181 gtgatcacag aagccacgga ccgtcgccgc ctttaccacc gccgcacaat cttatttgtt
      241 gatgaagtac accgctggaa caaagcccag caagacgcgc tgctacctca cgtggagaac
      301 gggatggtca cgctcatcgg cgccaccacc gaaaaccctt acttcgatgt cattcccgcg
      361 cttgtatccc gttcacgcat tttccagctc gaaccgctta ccgaggaaca gctggcggtc
      421 atctttaaac gcgcactggc agatgaggag ttcggttatg gcaagcgcca ggttctcttt
      481 gatgaaaaag cccgcgagca cctgctgcac caggctggcg gagacgcgcg taacctgttg
      541 aacgccatcg aattggcggt tgaaacgacc ccggcaggtg atgacggcgc catctacatc
      601 acgctggacg tcgcgcaaga atccatccag aagcgcacgc tgctttatga tcgcatgggc
      661 gatgagcatt atgacactat ctctgctttc attaaatccg tgcgcgggtc tgaccccgac
      721 gcagcgctgt actggctggc taagatgatc ttcgccggtg aagacccacg cttcatatta
      781 agacggttga tcgtgttgag tggagaggat atcgggctgg cagacccgca ggctctccag
      841 gtggttgcgg cggcggctta cgcgtatgaa tacatcggca tgcccgaggg gatttacccg
      901 atcgtcgagg ctacgttgta cctggctacc gcccccaaat ccaattcaac cggcgcatac
      961 ttcgctgccc gggctgagat cgaacagaac ggcgcggggc cggtgcctgt gcacctgatg
     1021 gactccaacc gcgatgccaa agggttcggt cacggcaagg gnnnnnnnnn nnnnnnnnnn
     1081 nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     1141 nnnnnnnnnn nnnnnnnnnn ngccaggtgc gcaagcagta acgcgcgtcg gtctgcatcc
     1201 gttgccccgg ggagcgggga gaagcgcgcg gagtgcagcc ccggcgcacc ttcaagcgct
     1261 tcaacttcca gcccggtatc gtcagccagt actgccaacc cggtggcagc ctggtaggca
     1321 aaggctttca ggagcgcgtt ctcaaggtat gtacggccgg tttcttccac ccgcgtgtca
     1381 ggagaaacct cctgcggggt cagcagttgc agcggcaggc ctgccaatag agcgctcaat
     1441 tcacctattt tgcctttatt ggtcgtcgcc agcagcagct gcataccttt attattgttt
     1501 atatttatca taattataat aataaattga cttcaactac ccaaagtgct ataatcgtga
     1561 ttgttaagat tatatcccga ttaataaccg tgtgccgtgg ccaaccaacg taacccatta
     1621 aaaattaatg tcggcttctt taataatctg ccgatcggga gttttcgcga cattcatttt
     1681 gaaagtcccg atttgcacct gcctccagac ctggacgtaa ccaatttttc agggagtgtt
     1741 catatttcgc ggaccccaca ggggttgctg tttgacgggg cttttcgcgc cttcattcag
     1801 gctgaatgtg tccgctgcct ggaaccttat gaccagctgc tcgagactga attcagtgaa
     1861 gtgtacgctt ataaatctca ctcctttact gaatccaacc tttacgttcc tgatgatggg
     1921 aacatcgacc tctcgccggt tatcagggag tacctgatgc tggaaaaccc gattaaaccg
     1981 ctatgtaaac ccgattgcca gggtttgtgc gtagtgtgcg gggagaatct aaacatcgca
     2041 acttgtgaac accaggccag gattaagatc gagtaaacca tttctggatg caccaggggc
     2101 aagaatatta aaataaagga gactctcagg tggcaaatcc cggaaggcga tccaagagcg
     2161 cggaagtact gaatttatta ctggaaaaag ctgacgtgca ggggtacctc acaacggaag
     2221 acttgatgga gttttaccca gacgtgacca gagacgcaga gcggattgaa gcggtactca
     2281 ctgccctgcg gcgccgaggt gtggaaatat tggatggtga gaacgactca gatatcactg
     2341 acaatgaagg ttacgcgcaa gtaaccgacc tggactttac ctccaccttt gagaacatct
     2401 cctctgacga cacgatcggc ctttacctta aggaaatgtc acgcgttcca ctgttaaagg
     2461 tagatgaaga gatcgatatc gcacggcgta tcgaagatgg acgttcgagc aagagggagc
     2521 tggaacgttc aaacggcggc tgcccggaga aagaaaaaca tttgctgcag cagcaagttg
     2581 aagatggctt gctcgcccgc gaacacctca ttaaggccaa tacccggctg gtggtaagtg
     2641 tggctaaaaa atacatcggc cgcggcgtgc cttttttaga cctgattcag gaagggaacc
     2701 tgggcttgat gaaagcggtg gaaaaatttg actatcaccg cggtttccgt ttttctacct
     2761 atgccacctg gtggatacgg cagaccatca cccgctctat cgcagatcag ggacgcacca
     2821 tccgtgtgcc agtgcatatg gtggaccgca ttcgccagtt gtacaaactg acgcacgaga
     2881 tgga
//
LOCUS       DAOTLW010000090         2881 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_28261_length_2881_cov_9.929229, whole genome shotgun sequence.
ACCESSION   DAOTLW010000090 DAOTLW010000000
VERSION     DAOTLW010000090.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2881)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2881
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28261_length_2881_cov_9.929229"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            64..1077
                     /locus_tag="P5516_08670"
     CDS             64..1077
                     /locus_tag="P5516_08670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT92541.1"
                     /translation="MNSPKKYVDWEPLNAVPGLRFRHFSGEADYQIRLDIFNATKEIN
                     GIDYVLTLEDFKNDAKWTKNYDIYQHLLYVEVEDKPVGYFGYSWDREPDGKVIYGPFG
                     VLRQEYWGRGIASLMLRFAEDKCKEIAERKHAGMDRRYRIWRKKKAVKTVQFLLDKGY
                     TIERYFLGMNRPIDLPLGEHPLPPGLEIRPVEPAHYRSIWNADSESFKDHWGYSEPTE
                     EMYEAWQNDRMFQPHLWKIAWDGDQVVGVVQNMVDEEENRVYNRKRGYTENISVRRPW
                     RGKGVASALIAESIRMFREMGMDHTHLSVDADSLTGALRLYQNIGYEVDDTQTSYNLC
                     KLF"
     gene            1085..1816
                     /locus_tag="P5516_08675"
     CDS             1085..1816
                     /locus_tag="P5516_08675"
                     /inference="COORDINATES: protein motif:HMM:NF024109.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HRT92542.1"
                     /translation="MKSTPRQLPTRYLALPTLRVAYCEAGSGANLILLHGNSENKAIF
                     KQYQAEYFTDYHTYALDSRGHGQSRSVDRAYSIAQYCQDVIDFCQARGIRKTHLVGYS
                     DGGNIALLLAKNAPQLFERIVSISPNYLASGSEERFLQAITRRYERMKRWNRWGWPNR
                     KKIMRYELMLNDIGISSEELKGIRTGMKILYAEREMIKEAHILEIAALIPHSELQKIK
                     GCHHLNILEQPETVAAIRAYLKTAS"
     gene            complement(2036..2854)
                     /locus_tag="P5516_08680"
                     /pseudo
     CDS             complement(2036..2854)
                     /locus_tag="P5516_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012692.2"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
BASE COUNT          748 a          780 c          767 g          586 t
ORIGIN      
        1 agccaagata tcgccgtata ataagcatta ccgtgagtat caaggtaaca gaaggaatta
       61 agaatgaact cccctaaaaa atatgttgat tgggaacctc tgaatgctgt tcccgggttg
      121 cgcttccgtc acttctcggg cgaagcggat taccaaatac gcctggacat attcaatgcc
      181 accaaagaga tcaatgggat tgattatgtg ttaaccctgg aagatttcaa aaacgacgcg
      241 aaatggacca agaactacga catctatcag cacctcttgt atgttgaagt agaagataaa
      301 ccggtagggt atttcggcta ctcatgggat cgcgaaccag acggcaaggt catctacggt
      361 ccattcggcg tgctgcggca ggaatactgg gggcgtggca ttgcctcctt gatgctgcga
      421 ttcgcagagg acaaatgtaa agagatagcc gagcgtaaac acgcgggtat ggaccggcgc
      481 taccgcatct ggagaaaaaa gaaagccgtt aaaacggtcc agtttctgct ggacaaggga
      541 tataccattg aacgctactt tctcgggatg aaccgtccca ttgacctgcc gctgggtgaa
      601 catccccttc ctcccgggct agagatccgt ccggttgagc ccgcccatta ccgctcgatc
      661 tggaacgcgg atagtgagtc tttcaaagac cattgggggt attctgaacc aacagaagaa
      721 atgtatgaag cctggcagaa tgaccgtatg tttcagccac acctgtggaa gatcgcctgg
      781 gacggcgacc aggtagtcgg agtagtgcaa aacatggtgg acgaagaaga gaaccgcgtc
      841 tacaaccgta agcgcggcta caccgagaac atctcggtgc gccgcccctg gcgtggaaaa
      901 ggagttgcca gcgccctcat tgctgagagc attcgtatgt tccgtgaaat gggcatggac
      961 cacacccacc tatcggtaga cgcggacagc ctcaccgggg cgttgagact atatcaaaat
     1021 atcgggtacg aagtggatga cacgcaaacc agctacaacc tctgcaagct cttctgagga
     1081 accggtgaaa tcaaccccgc gccaattgcc cacccgctac ctggctcttc caaccttgag
     1141 ggttgcgtac tgtgaagccg gcagcggggc caacctcatc ctgctgcatg gcaattcaga
     1201 aaacaaggcc atcttcaagc aataccaggc ggaatatttt actgattatc acacctacgc
     1261 actcgatagc cgcggtcacg gtcaaagtcg gtctgttgac cgggcgtatt caatcgcgca
     1321 gtattgccag gatgtgatcg acttctgcca ggcgcggggc atccgtaaaa cccacctggt
     1381 ggggtacagc gatggcggca atattgcgct gctgctggcg aagaacgccc cgcagctttt
     1441 tgagcgtatc gtctctattt cgcccaacta cctggccagc ggcagcgaag agcggttttt
     1501 acaggcaatc acacgccggt atgaacgtat gaagcgctgg aaccgctggg gatggccaaa
     1561 ccggaaaaag ataatgcgct acgaattgat gctgaatgac attggcatca gcagcgaaga
     1621 gttgaaaggg attcgtaccg gtatgaagat tctctacgcc gaacgcgaga tgatcaagga
     1681 agcgcacatt ctcgaaattg ccgcgctcat ccctcacagc gaactccaga agatcaaagg
     1741 ctgccatcac ctgaacattc tcgaacaacc ggagacagtg gctgccatcc gagcctacct
     1801 gaaaacagcc agctgatcct tgacgcggta aaagggaagt gcgcaaatga tgaataaaac
     1861 gcgcgcttct atcctccaaa atactaaggg aaatgagagg tttttagaac gcgaattttc
     1921 caggacgcgg gtttgtacgt cgttccctca accacgatga catccgcgct ctcaatggag
     1981 ccgcgacctg ttgcgcaccc tgctaagcct tcagaaacgt atcttacgaa aatctctata
     2041 ggggctcgag gttgcctaac ctcatcgtca tggcaatggc gcccagcacc cccgagtatc
     2101 tccccagcgc gggagggaca atatattcac ccatgccaga aaggatagcg tcctcgcgaa
     2161 tgtaaccgtt tagcagctca gtgaccctgc tcctgatgag cgggaataag aataaccgct
     2221 gcataattcc gccccctaac acgatgatgc gtggagaaag cgtgaggatc aaattggtca
     2281 gcgcgctggc gatatactcc acctccagca gccagaacgg atcctcatcc gtcaggtctt
     2341 caccacgttt gccaaaacgg gcagttatcg ctggcccggc agccagaccc tcaaaacagt
     2401 cccggtggta cgggcagctg ccttcaaacg ggtccttcag cctgtcgtga gggatgcgta
     2461 tatgccccat ctcaaccgcc gccggccctg atagcggtgc gccgttcaac aggtacgcgc
     2521 caccaatccc ggttcccact gtgagatagg gggaagggtc aattcccctg gacgcgcccc
     2581 agatatactc cccgatggcg gagccgctga catccgcgtg gaaggcaatg ggcacgccca
     2641 attactgctg aagttcacca aggatgttgt attctctcca tcctggcttg ggcgtggtgg
     2701 taatgtagcc ataagtcggg gaaaggggat tcacctccac agggccaaaa gaggcgagcc
     2761 cgatacccgc aaccatcccc tgcttaataa aaggctcaaa gaaaaggcgc acctgttcca
     2821 gtgtgctgga gggatcggtt gtactgaagg tcacttgatg atccaaccgt ggaggttctg
     2881 a
//
LOCUS       DAOTLW010000091         2875 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_28360_length_2875_cov_8.414894, whole genome shotgun sequence.
ACCESSION   DAOTLW010000091 DAOTLW010000000
VERSION     DAOTLW010000091.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2875)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2875
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28360_length_2875_cov_8.414894"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /locus_tag="P5516_08685"
     CDS             <1..242
                     /locus_tag="P5516_08685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT92543.1"
                     /translation="HSPGSVVFYMESLHTAVVGDLIFHQGVGRTDLPGGSFEVLKTSI
                     ITQIFSLPPQTVLIPGHGPFTTVESEMQLNPYFQK"
     gene            263..1201
                     /locus_tag="P5516_08690"
     CDS             263..1201
                     /locus_tag="P5516_08690"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HRT92544.1"
                     /translation="MGKYFRGLGFALILITSVVITGCSHQTVSSSAELTATMTPFQPQ
                     VPASATPTTTPPPTPTPLPTEETETPEEHLQFAPQELGPGEVTVPILLYHHVSDTLQI
                     QYSVTTSALDEQMNWLYQNGYQTITVTELAHLIREGGTAPGRPVIITFDDGWLDLYEN
                     AYPILRKYGFQGTAYIVANAVNAQGNLSAEQLKELIDQGWEIGSHGLNHVDLTKSDGW
                     QEEITASKAYLEKVLGIEILTFAYPYGVADIGVMNYTFSAGYTSAVGLGSTATHSRST
                     LYYLSRMEVKSWHGLVFLEEIMPWSEKKGSPVSESP"
     gene            complement(1217..2755)
                     /gene="rny"
                     /locus_tag="P5516_08695"
     CDS             complement(1217..2755)
                     /gene="rny"
                     /locus_tag="P5516_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - endoribonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HRT92545.1"
                     /translation="MGKIETGLILLFVLGALLLAALLTILLVNVYKSKFKKEQSEKAD
                     AVILTAVEKAKTIELEARDKALKVLQESEQEANRRRQDILREDDRQQKRRAELDHRLE
                     RLELREQNLNKRQSALDKRANEIEKLYADQLEELQRIAQMTMDDAKQVLMTEAEKEAR
                     NDMARIIRSIEAEARAEGDKRAREIITDAIQRVASEHVVSVSTSLVTLPNEEMKGRIV
                     GRNGRNIRAFEQAAGVDVIVDDTPEAVTISCFDPVRREIARRSLGRLINDGRIHPAHI
                     EKILAEEEREVEKAIVEAGEEAAFDAGVAGMHPEVLKMLGRLKFRTSYGQNQLAHAVE
                     VARLSAVLAAELGADVEVARTGGLLHDLGKAMDHNTEGTHAQLGADFARRYGVNSRVV
                     NAMASHHHEVEQESIEAVIVEAADAISGARPGARREDLEQYIKRLRALEEIANSFKGV
                     QQSYAIQAGREVRIIVQPDEIDDLASTRLARDIAKKVEESMQYPGQIKVTVIRETRAV
                     EFAK"
BASE COUNT          620 a          842 c          646 g          767 t
ORIGIN      
        1 gccactcacc tggttctgtt gttttttaca tggaaagtct ccatactgcg gtagtgggcg
       61 accttatttt ccatcaggga gtggggcgca ctgatctgcc aggtggtagt tttgaagtgt
      121 tgaaaaccag catcattacc caaatcttct cgctgccacc gcaaacggta ttgatcccag
      181 gtcatggtcc cttcaccacc gtggagtcag aaatgcaact caatccctat tttcaaaaat
      241 aattgattat aatttctgca ctatgggcaa atattttaga ggtttaggct ttgctcttat
      301 tttgatcacc tccgtcgtca tcactggttg ctcacaccag acggtcagtt catcagcaga
      361 attgacggca acgatgaccc cgtttcagcc ccaggttcct gcttccgcta ctcctactac
      421 cactccccct cctacaccca cgcctttgcc aaccgaagaa accgaaacgc ctgaagaaca
      481 tctccagttt gccccgcaag agttggggcc cggcgaagta accgttccca tattgctgta
      541 tcaccatgtt tctgataccc tgcaaatcca gtattcggtg accaccagcg cgttggatga
      601 acagatgaac tggctttatc aaaatgggta tcaaaccatc accgtcaccg aactggctca
      661 tctgatccgc gaaggtggaa ccgcccccgg gcgaccggtc atcatcactt tcgatgatgg
      721 ctggctcgac ctttacgaaa acgcctaccc gattttgcgc aaatatgggt tccagggaac
      781 cgcgtatatc gtcgccaatg cggtgaatgc gcaaggcaac ctcagcgcgg agcagttgaa
      841 agaattgatt gaccagggtt gggagatcgg ttcgcatggc ttaaaccacg ttgacctaac
      901 caagagcgac ggatggcaag aggagattac cgcttccaaa gcatatcttg agaaagttct
      961 cgggattgag attctcacct tcgcctatcc ctacggcgtg gcagacattg gcgtaatgaa
     1021 ctataccttc agcgcgggat acacctccgc ggttggattg ggctcaacag cgacacatag
     1081 tcgatccacc ctctattact tgagtcgcat ggaagtgaaa agctggcacg gtctcgtttt
     1141 cctcgaggag atcatgccct ggtcagaaaa aaaaggctcc ccggtttcgg agagccctta
     1201 attcagtctc tacattttat ttggcaaatt ccacagcgcg agtttcgcga ataacggtca
     1261 cttttatctg acctggatac tgcatactct cttccacctt cttggcaata tctcgcgcca
     1321 ggcgggttga agcgaggtca tcaatttcgt cgggttgaac gatgatgcgc acctcgcgcc
     1381 cagcctgaat ggcgtaactt tgttgtactc ccttgaagga gttagcgatt tcttccagcg
     1441 cgcgcaggcg tttgatgtat tgttccaggt cttcacgcct ggcccccggc cgcgcgcctg
     1501 agatagcgtc agccgcttct acaatgaccg cctcaatgga ttcctgttcc acctcgtgat
     1561 ggtgggaggc catcgcgttc accaccctcg agttcacccc gtaacgccgg gcaaaatccg
     1621 cgcctaactg agcatgcgtg ccttcggtat tgtgatccat cgctttaccc aggtcatgca
     1681 gtaaaccgcc tgtacgggct acctcaacat ctgcgcccaa ttcagcggcc aacaccgctg
     1741 aaagcctggc cacctccacc gcgtgcgcca gctggttctg cccgtaagat gtgcggaact
     1801 tcaacctccc gagcattttc aacacttcgg ggtgcatccc tgctactccg gcatcaaagg
     1861 cggcttcttc gcctgcctct acgatggctt tctctacttc gcgttcctct tcagccagta
     1921 tcttttcaat atgagcggga tgaattctgc catcattgat caggcggccc agtgaacgcc
     1981 tggcgatttc tctcctcaca gggtcaaaac aactgatggt cactgcttcg ggtgtatcat
     2041 ccacgatgac atccacccca gccgcctgtt caaaagcccg gatatttcgc ccattacgcc
     2101 caacaatgcg gcctttcatt tcctcgttag gcagcgtcac cagcgaagta gaaacgctca
     2161 ccacgtgttc cgaagcgacc cgttgaatgg catccgtgat gatctcgcgg gcgcgtttat
     2221 ctccttcggc tcgggcttca gcctcgatgg aacgaataat gcgcgccata tcattacgcg
     2281 cttctttttc tgcttcggtc attaagactt gcttggcatc atccatcgtc atttgagcga
     2341 tgcgttgcaa ttcctccaac tggtcagcgt acaacttttc gatttcattg gctcttttat
     2401 ccagcgcgct ttggcgcttg ttcaaatttt gctcgcgtag ctcaagcctc tcaaggcgat
     2461 ggtccagctc tgcgcgcctt ttctgctgcc ggtcatcttc tcttaaaata tcctgccgcc
     2521 gccggttcgc ttcctgctca gattcttgca gcaccttcag cgctttgtcc cgcgcttcca
     2581 gttcaatcgt tttcgccttc tcaaccgctg taagtattac agcgtccgct ttttcacttt
     2641 gttctttttt aaattttgat ttgtatacgt ttaccagcaa tatcgttagc agcgctgcga
     2701 gcaacaacgc tcctaaaaca aacagtaaga tcagtcccgt ttcaattttt cccatgttta
     2761 ttcttcctct ttttcttcgt cagtattatt gttagcactt cgctgctccc acagggcgga
     2821 aaccaccggt gcgatgactt cataagaaaa acctcgcctt gccaggaacc cgctc
//
LOCUS       DAOTLW010000092         2862 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_28537_length_2862_cov_9.226933, whole genome shotgun sequence.
ACCESSION   DAOTLW010000092 DAOTLW010000000
VERSION     DAOTLW010000092.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28537_length_2862_cov_9.226933"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            424..2724
                     /locus_tag="P5516_08700"
     CDS             424..2724
                     /locus_tag="P5516_08700"
                     /inference="COORDINATES: protein motif:HMM:NF015714.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HRT92546.1"
                     /translation="MPLLWLSTAFFAGILLAGAFAPPGWVYAVVAAIGALLILAERLA
                     PISLSLLRNTRNLLRFPAGLLFVFLALGGLRYQAGQMKTNPDSLAYYNDRGVYTITAL
                     VSAAPDRREDAVYLELSALEIEDPRAAEPLLAVKRVSGKARARFAPGARWQAGDVLRF
                     SAAPLTPADEGAFSYKDYLERQHIATVIYDPRSVQLVGARGAWFSRGLERIRQRVKTA
                     LFALYPQPESGLLAGILLGLDQDLPESLEKAYQQTGTAHIIAISGFNMAVLAFLFMWV
                     FNRLFNRYWAAALSALFIALYAVFVGGTASVVRAALMAVSAFGGHLIGRRQSGINALG
                     FTAALMCVFNPTLPWDVSFQLSFAATLGLMLFAEPLHQQAVELLQKRLPENQAHKIAA
                     PLSEYFLYTLAAQLATLPIIAWHFGRVSLSSLLANPLVLPAQPPLLVLSGLSAVTGTL
                     LPAAGKVLAVFAWPLAAYTNRVVALLSRFNTASLALNHSTALWLLVFVVVFVLLFLLR
                     GYFKKIFKGNGYWLLFLLVLACFSVWSMLMHRPDGRLHLRLLRAGDESTLTASTPAGR
                     VLVFDPRGNLNDLSAAVGQHLSPWRYYIDETWFAQPAPAAVLERLQERVPLRSVVLAP
                     VIYRAGAGVKPFSAPQGIEVIRLQPGGGLRYSHSLDIRVVAESNSAAALLITYDQVKV
                     LIPNGVDYALIKATSPLALQALSLVVVGEEDISYIPPRVWQALAPAAILWRDTTVCPV
                     EGWLNLGAGDALELVADGKTLSLAKQ"
BASE COUNT          503 a          894 c          813 g          652 t
ORIGIN      
        1 aaggaaaaaa tctaaagacg ataccccgca gcttgctgcg gggaggattc atttctattt
       61 ttatccagcc gatctatcca atctacgatt tcttgagatg taatcccggg cattttttct
      121 cctaagacaa gtagcaatta ctattgtact agaatgtaat aaaggctaat tttctagagt
      181 cattctttct tccgctagct tcgaggttcg agtcctcgac gcggagcaaa aaacaccctt
      241 aaggtttttt tacaggtctt ctttccgatc gaaaccgacg ccaattgaca ttcagaccct
      301 cataactgag tatagtcata ttaccctccc aagaacagta tataacgttc ttaaaaaaag
      361 acattcatta acaaaacaaa aataccctat aatctttcca acagttggtc attttgagct
      421 gccatgccct tgctctggct ttccaccgcc ttcttcgccg gcatcctgct ggctggcgcc
      481 ttcgcgccgc ccggctgggt ttatgccgtc gtcgcggcaa tcggcgcact gctcatcctg
      541 gctgagcgcc tggcgcccat ctccctttct ctgctgcgca atacccgcaa cctcctgcgc
      601 ttccctgccg ggctgctgtt cgtcttcctc gccctgggcg ggctgcgcta ccaggcgggt
      661 cagatgaaaa cgaaccctga ttcactggcg tactacaacg atcgaggtgt ttacactatt
      721 accgctctcg tcagcgccgc gcccgaccgc cgggaagacg ccgtctacct tgaactcagc
      781 gcgctggaaa ttgaagaccc ccgcgccgcc gaaccgctgc tggcggtgaa gcgtgtgagc
      841 ggtaaagccc gcgcgcgctt tgcccccggc gcccgctggc aggcgggcga tgtgctgcgt
      901 tttagcgccg cgcccctcac ccccgcggat gaaggcgcct tctcttacaa agattacctg
      961 gagcgccagc acatcgccac ggtgatctat gacccgcgct cggtccagct ggtgggcgcg
     1021 cgcggcgcct ggttcagccg cgggcttgaa cggatccgcc agcgggtgaa gacggcgctc
     1081 ttcgcgctct acccccagcc cgagtcaggg ctgctcgcgg gcatcttgct ggggctggac
     1141 caggacctgc cggagagcct ggaaaaagcc tatcagcaga ccggcacagc gcatatcatc
     1201 gccatctccg ggttcaatat ggcggtgctg gctttccttt ttatgtgggt ctttaaccgc
     1261 ctgttcaacc gttactgggc ggcggcgctt tccgcgctgt tcatcgccct gtacgcggtc
     1321 ttcgtgggcg gcacagcctc ggtggtgagg gcggcgttga tggcggtcag cgcctttggt
     1381 ggtcacctca tcgggcgcag gcagtccggg atcaacgcgc tggggttcac cgctgcgctc
     1441 atgtgcgtgt tcaaccccac gctgccctgg gatgtgagtt tccagctctc cttcgctgcc
     1501 acgctggggc taatgctctt cgccgaacca ctgcaccagc aggcggtgga gctgctgcaa
     1561 aaacgcctgc ctgaaaacca ggcgcacaag atcgccgcgc cgctcagcga atacttcctc
     1621 tatacgctgg ccgcgcagct ggcgaccctg ccgatcattg cctggcattt tgggcgcgtc
     1681 tcgctttcct cgctgctggc caacccgctg gtactgccgg cgcagccgcc gctgctggtg
     1741 ctgtcgggcc tcagcgccgt tacaggcacg ctgttgcccg ccgccggcaa ggtgctggcg
     1801 gttttcgcct ggccgctggc cgcctatact aaccgcgtgg tggcgctgct ttcccgcttt
     1861 aacactgcca gcctggcgct caaccattcg accgctctct ggttgctggt atttgtggtg
     1921 gtttttgttc tgctcttcct tttgagaggt tatttcaaaa aaatattcaa agggaatggc
     1981 tactggctgc tctttttgct ggtgctggcg tgtttttcgg tgtggtccat gctgatgcac
     2041 cgccccgacg gtcgcctgca cctgcggctg ttacgcgccg gagatgaaag cacactcacc
     2101 gcgagcaccc cggctgggcg cgtgctggtg ttcgacccgc gcgggaacct gaacgacctg
     2161 agcgctgcgg ttgggcagca cctttctccc tggcgctatt acattgatga aacttggttt
     2221 gctcaaccgg cgccggcagc cgtgcttgaa cggttgcaag aacgcgtacc gctgcgctcc
     2281 gtggtgctgg cgccggtcat ctaccgcgcc ggcgctggcg taaagccctt ttcagcgccg
     2341 caaggcatcg aggtgatcag gctgcagccc ggcggggggt tgcgttactc gcactcgttg
     2401 gatatccgcg ttgtggctga aagcaactcc gccgccgccc tgctcatcac ctatgaccag
     2461 gtgaaggtac ttatccccaa cggtgtggat tacgcgctca tcaaagcaac ctcccctctg
     2521 gcgctgcagg cgctttccct cgtggtggtg ggcgaggaag atatcagtta catcccgccg
     2581 cgcgtgtggc aggcgctcgc acctgcagcc atcctgtggc gtgacacaac ggtctgcccg
     2641 gtggaaggct ggctcaacct cggggcgggc gacgcgctgg aactggtcgc ggatggaaaa
     2701 accctctccc tggcaaaaca atgagcacgg ggctcacgaa agaaaactcg tgaaagaccg
     2761 cccatcgcgc tggcgttaag ccagttgaag ccgtggcgct gttggttggc gggtgcacct
     2821 ctcttcaatg atcaattttt ctggcgcggc aaaggttata at
//
LOCUS       DAOTLW010000093         2842 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_28775_length_2842_cov_5.728741, whole genome shotgun sequence.
ACCESSION   DAOTLW010000093 DAOTLW010000000
VERSION     DAOTLW010000093.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2842)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2842
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28775_length_2842_cov_5.728741"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            381..2816
                     /locus_tag="P5516_08705"
     CDS             381..2816
                     /locus_tag="P5516_08705"
                     /inference="COORDINATES: protein motif:HMM:NF012311.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HRT92547.1"
                     /translation="MKLYKVFSLFMVLCFMLSMTVSAKPVNAQVEPPADPGEFIPGEV
                     VVMFKPGMKISEYAASAADLAGRTQTTAVKVSKEGMALLRGDASVNISQLIETIKANS
                     NVLFAEPNYVYRIPSPEVSPEQDTLRHEFVFRTSVSSETGEKETTVIPVSYLQNLKSV
                     RGGKITATYPNDPYLWSNWGWDYVDADVVSPNTTASAGVCVLDTGVDYLHPDLSGNII
                     KGYDFVNADTDPMDDNGHGTHVAGIITAKPNNKIGLAGASNAKVVAVKVLGAQGWGTS
                     YNIAAGINFCANRSDVKVLNMSLGGTIYSVAIDNAVAYAVNTKGKLVVAAAGNRDTDT
                     PSYPAGLSLTYPGKVLSVAASGSLEWDDEYEEWFLEDESCRANNSNYGNWVNIIAPGT
                     DIFSTLPYDKPFYLNYFEGMNPRYGTLSGTSMAAPFVAAAAARRWGYKPKSTNVEVFD
                     AVVNSGWDVLGDDSCWPISMHGRHIVNIANLLDRYAVEAGIHDASSGVGLDGAALQVY
                     KGKTLLGSAVVTPFVWSYISRQYKILDEFKYYTAWTTVINLPAPTDGTPFWDWGNGHT
                     YKVSKSGYTSSPQEAFQHNNYRWYSTASFMFPEPAALPPKSTNLEVVMGWHQNYDYPY
                     WEADPDLDLYVWLPSVPNPLDGSQPAPFIVGPEGISFGFLEGDGTGSMNYFPFAHLKR
                     EGGGVDYLAVESTTISSRKAHAPLAANPALPYYPGDYVIGVTDWGQTFDHDDDAGTPE
                     IPVMGAAYVPYLYIWKDGVIKFFNQMPYQDAGDECNEGWWRGATISSGKTGAITYTPQ
                     NVCSDSTLGLFPY"
BASE COUNT          696 a          705 c          768 g          673 t
ORIGIN      
        1 aaacacctga attattcaat agggacgctg tttgccaggt ttgtcacagg gtgaaatgta
       61 aaaggcatcc aggctcaccc atggaagatg tgacataata gaccctttcc cggttgggtt
      121 tgccagcttc aatggtggct ggagctcaat atggtccctc tcttctatgg ttatttgtgc
      181 ctagattcca tagagcaaaa tgattgaatt tgatattctt ttgtgctaaa ataattaatg
      241 gtagtttcac actgtgaatt caaagcaaag accatcttaa ccttcagggg aggtgtctat
      301 agagaggtca tttccagtta agcgtataat cgcgccggtc atcgtgaaag tcatctcttt
      361 tctaaccaag gagtatactc atgaaactct ataaagtttt cagcctattt atggttcttt
      421 gttttatgtt gtcgatgacg gtttcagcga aaccggtcaa cgcccaagta gaaccgcccg
      481 ctgaccccgg cgaattcatc cccggtgaag tggtggtcat gttcaaacct ggcatgaaga
      541 taagcgagta cgccgccagc gcagctgacc tggcggggcg tacgcaaaca acagcggtga
      601 aagtctccaa agagggaatg gcgctgctgc gcggcgacgc cagtgtgaac atcagccaac
      661 tgatagagac cattaaggca aattcgaacg tgctcttcgc ggaacccaac tacgtgtaca
      721 ggatcccatc gcccgaagtc tccccggagc aggatacgct gcggcatgaa ttcgtgttca
      781 gaacttctgt ctcctctgaa accggcgaga aagagaccac ggtgatcccg gtctcttatc
      841 ttcagaacct gaagtcagtg cgcggcggga agatcacagc gacttatccc aacgatccct
      901 acctgtggtc gaactggggc tgggattacg tcgacgcgga tgtagtttcg cccaacacca
      961 ccgccagcgc gggagtttgc gtgctggata ccggagtgga ttacctccat cctgatctat
     1021 cgggcaatat catcaagggc tatgattttg tgaacgctga cacggacccg atggatgata
     1081 acgggcacgg cacgcatgtg gcgggcatca tcacagccaa gccgaacaat aagatcggct
     1141 tggctggcgc ttcaaacgcg aaggttgtgg ctgtaaaagt actgggtgcc cagggctggg
     1201 gcaccagtta taacattgcc gcgggcatca acttttgcgc caatcgcagc gatgtgaagg
     1261 tgctcaacat gagcctgggt ggcaccattt actcagtggc aattgataat gccgttgctt
     1321 acgccgttaa taccaagggg aaactggtag ttgctgctgc aggtaatcgt gatacagata
     1381 cgccatctta ccccgcgggg ttatcattaa cttatcctgg caaggtgctg tcagtcgccg
     1441 cctctggatc tctcgaatgg gatgatgaat atgaggaatg gtttttagag gatgagtcct
     1501 gccgggcgaa taactccaac tacggcaact gggtgaatat catcgccccg ggaacggata
     1561 tcttttctac cctgccgtat gataaaccct tctatctgaa ctattttgaa gggatgaacc
     1621 cccgctatgg gacgttgagc ggcacttcca tggcagcgcc gtttgtagcc gcggcagccg
     1681 cgcgaaggtg ggggtataaa cccaaatcca ccaatgtgga ggtgtttgat gcggtggtca
     1741 acagcggttg ggatgttttg ggagacgaca gttgctggcc aatctcaatg catggaagac
     1801 acatcgtcaa tatcgccaac ctgctggatc gctacgccgt agaagcgggg attcacgacg
     1861 ccagctctgg agtgggtctc gacggcgcgg cactccaggt ttacaagggg aaaacactat
     1921 taggttcagc ggtagtaact ccttttgtat ggagttacat ttcaagacaa tacaaaatcc
     1981 tggacgaatt caagtattac accgcgtgga cgacggtgat caacctgccg gcgcctactg
     2041 acggtacacc tttctgggat tggggtaacg ggcataccta taaggtgagc aaatcgggtt
     2101 acacatcaag cccgcaggaa gccttccagc acaacaatta ccgttggtat tccacagcct
     2161 cctttatgtt tcccgaaccg gcagctctcc ctccaaaatc cacgaacttg gaagtggtaa
     2221 tggggtggca tcaaaactat gactacccgt attgggaagc agatccggac ctggacctct
     2281 atgtgtggtt gcccagtgtg cccaacccac tggatggcag ccagccagct ccgttcatcg
     2341 tcggtccaga gggtattagt tttggttttc ttgagggaga cggtacaggt tcgatgaact
     2401 acttcccctt cgctcatttg aaacgagagg gtggtggggt tgattatttg gcggttgaga
     2461 gcaccactat cagctcgcgc aaggcgcacg cccctctggc tgctaatcct gccttaccct
     2521 attacccggg cgattatgtg attggtgtga cagattgggg tcagaccttc gaccatgatg
     2581 acgatgcagg aactcctgag atcccggtta tgggcgcagc ttatgtaccg tacctgtaca
     2641 tctggaaaga cggggttatc aagtttttca accagatgcc ctaccaggat gctggagatg
     2701 aatgcaacga gggctggtgg agaggtgcga ccatcagcag cgggaagacg ggcgcaatca
     2761 cttacacacc ccagaacgtc tgcagcgata gcacattggg gctcttcccg tactagaaaa
     2821 acaaaacggg gtcactccat cg
//
LOCUS       DAOTLW010000094         2829 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_28930_length_2829_cov_10.668349, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000094 DAOTLW010000000
VERSION     DAOTLW010000094.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2829)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2829
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_28930_length_2829_cov_10.668349"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="P5516_08710"
     CDS             <1..747
                     /locus_tag="P5516_08710"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT92548.1"
                     /translation="RKAWLVDKTVPLETVRFHHVSTDEVFGTLSKTDPPFREETAYQP
                     RSPYAASKASSDHFVRAYSTTYGLPISISNCSNNYGPRQFPEKLIPLMILNAISGKPL
                     PIYGDGMQIRDWLYVDDHCEALWKVVTQGKVGETYCIGGDNQPPNLEIVRTICQILDA
                     LQPDSPFTPHEQLMRFVPDRPGHDRRYDIDISKIKSELGWQPRNTLQDGLRATVQWYL
                     EHPEWVAAITQQSDYDGWMQKNYANRKEGA"
     gene            744..1610
                     /gene="rfbA"
                     /locus_tag="P5516_08715"
     CDS             744..1610
                     /gene="rfbA"
                     /locus_tag="P5516_08715"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016283109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HRT92549.1"
                     /translation="MKGIILAGGKGTRLYPLTIPISKQLLPVYDKPMIYYPLSMLMLA
                     GIREILVITTPEDQALYQRLLQDGTQWGLKFAYAVQPEPRGLADAFLVGKDFLAGSPA
                     CLILGDNIFFGQGLPTVLRNAAELQQGAVIFAYPVRDPERYGVVEIDGNGLALSLEEK
                     PKQPRSNLAVPGIYFYDANVVSYAEQLKPSARGELEITDLNRRYMEQGQLQVKVLGRG
                     VAWLDAGTHESLLQAATFIQAVEERQGMMISSVEEIAYRMGYIDREQLNKLAEGLNAT
                     NYGAYLRRLAED"
     gene            1613..2497
                     /gene="rfbD"
                     /locus_tag="P5516_08720"
     CDS             1613..2497
                     /gene="rfbD"
                     /locus_tag="P5516_08720"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HRT92550.1"
                     /translation="MLRILLIGRSGQLGWELQRCLAPLGEVVTYDYPEIDLAKPASLP
                     EFVRGVHPQVIVNAAAYTNVDKAESEAELARQINSVSPGVLAEEARRLGAAFIHYSTD
                     YVFDGCKGAPYTESDALHPLNVYGQTKLEGEQAVQAAGGAYLILRTSWVYSLRQGGFV
                     TKVLQWAREQETLRIVDDQISSPTWARMLAEATALIIAQGRDDPVGYLTEKAGLYHLA
                     GSGACSRFEWAQAILELDPKKSEQKVQQLLPAKSSAFPTPAERPLVSVLECGKFEKTF
                     GLRLPEWRGALKLAINRD"
     gene            2524..>2829
                     /locus_tag="P5516_08725"
     CDS             2524..>2829
                     /locus_tag="P5516_08725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92551.1"
                     /translation="MGLQNYLRRNLRRVGYQVGFKKFTADLALIKNSGYFDDNWYRQN
                     NPDVFEAGADPLRHYLEYGGFEGRTPGPKFDSAGYLDTYADVRRSGVNPLVHYLKFGR
                     "
BASE COUNT          652 a          732 c          817 g          628 t
ORIGIN      
        1 cgcaaagctt ggctggtgga taaaaccgtt ccgcttgaaa cggtacgttt tcatcatgtc
       61 tccactgacg aggtctttgg cacgctcagt aaaacggatc cgccctttag ggaggaaacc
      121 gcttaccaac cgcgttcgcc ttatgcggct tccaaggctt cgagtgatca ttttgtgcgc
      181 gcttactcca cgacttatgg cttgcccatc agtatcagca actgctccaa taactatgga
      241 ccccgccaat tccccgagaa actcatcccg ctcatgatct tgaacgcaat cagcggaaaa
      301 cccctgccca tttatggcga tggcatgcaa atccgcgact ggctgtatgt ggacgatcac
      361 tgtgaagcgc tctggaaagt agttacccaa ggcaaggtcg gggagaccta ctgtatcggc
      421 ggggataacc agccgcctaa tttagagatc gtgcgcacga tttgccaaat cctggatgcc
      481 ctgcagcccg actcgccttt cacgccacac gagcaactaa tgcgttttgt acccgaccgc
      541 cctgggcacg accggcgcta tgatattgat attagcaaaa taaagagcga gctcggttgg
      601 cagccccgca acaccttaca ggatggcttg cgagccacgg tgcagtggta tttggaacat
      661 ccggaatggg tggcagccat cacccaacaa tcggattacg atggctggat gcagaaaaat
      721 tacgcgaatc gcaaggaggg agcatgaaag gcatcatctt agctggcggc aaaggcaccc
      781 ggctttaccc actcaccatt ccaatcagca agcagctctt gccggtatac gataaaccca
      841 tgatttatta ccccctctcc atgctcatgc tggcgggcat tcgggagatc ttggtgatta
      901 cgactcccga agaccaagcc ctctatcagc gcctgctgca ggatggcacg cagtgggggt
      961 tgaagttcgc ttacgccgtg cagcctgaac cgcgcgggtt ggcggatgca tttttagtgg
     1021 ggaaagactt tttggctggc tcaccagcct gcttgatctt gggcgacaac atcttctttg
     1081 ggcaagggct gccgacggta ctgcgtaacg cggctgaatt acagcaaggt gcagtgatat
     1141 ttgcttaccc tgtgcgtgac ccggagcgct acggtgtagt ggaaatagat ggaaatggtc
     1201 tggcgctcag cttggaagaa aaacccaagc agccacgctc aaacttggct gtgcctggga
     1261 tttatttcta tgatgcgaat gttgttagct atgctgagca gctcaaacct tcggcgcgcg
     1321 gcgagctgga gattaccgac ctcaaccgac gatatatgga gcaagggcag ctgcaggtga
     1381 aggtgctggg gcgcggggtg gcttggctgg acgccggcac ccatgagtcg ctgctgcaag
     1441 cggctacttt catccaggct gttgaagagc ggcagggcat gatgatctcc tcagtggaag
     1501 agatcgccta ccgcatgggt tacatcgacc gtgagcagct gaacaaatta gcagaggggc
     1561 tcaatgctac caattacggc gcttacctta ggcgcttggc agaggattag ttatgctgcg
     1621 cattttattg attggcagaa gcggacagct tggctgggag ctacagcgct gcttggcacc
     1681 gctgggcgag gttgttactt atgactaccc cgaaattgac ttagctaagc ccgcaagctt
     1741 gccagagttc gtgcgcgggg tgcacccgca ggtgatcgtc aatgcagcag cctataccaa
     1801 cgtggacaaa gctgaaagcg aagctgagct tgcccggcag attaattccg tttctccagg
     1861 cgtgctagca gaggaagcca ggcggctagg tgccgctttt atccactatt ccaccgacta
     1921 cgtgtttgac ggctgcaagg gggctccata cacagaaagt gatgcgctcc atccgctcaa
     1981 cgtctacggg cagaccaagc tggaaggcga gcaggctgtg caagccgctg gtggcgctta
     2041 cctcatcttg cgcaccagct gggtctattc cctgcggcag ggtggttttg ttaccaaggt
     2101 gctacagtgg gcgcgggagc aggaaaccct gcgtatagtt gatgaccaga tcagcagccc
     2161 cacctgggcg cgcatgttgg ctgaagccac tgctttaatt atcgctcagg gacgagatga
     2221 cccggtgggt tatcttacag agaaagccgg gctgtatcac ctagccggct ctggcgcctg
     2281 cagccgattt gaatgggcgc aagccatatt ggagctagac cccaaaaaga gcgagcagaa
     2341 ggtgcagcaa ttgctgcctg ccaagagcag tgcgttcccc acgccggcag agcgtccgct
     2401 cgtatcggtg ctggaatgcg ggaagtttga gaaaactttt ggcttgcggc tgcccgagtg
     2461 gcggggagcg cttaaattgg caataaaccg tgattaaaga ttattaattg gggagttggt
     2521 gaggtgggtt tacaaaatta tcttcgaaga aaccttcgca gagtaggata ccaggtcggg
     2581 tttaagaaat ttacggcgga cttagcgcta atcaagaact cgggttactt cgacgacaat
     2641 tggtatcggc agaataatcc tgacgtattt gaagccgggg ccgatccgct gcggcactat
     2701 ctggaatatg ggggctttga aggccgtaca cccggaccaa aattcgatag tgctggctac
     2761 ctggacacgt atgcggatgt gcgccgctcc ggggttaatc cactcgttca ttacctgaaa
     2821 tttggccgg
//
LOCUS       DAOTLW010000095         2798 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_29339_length_2798_cov_8.639446, whole genome shotgun sequence.
ACCESSION   DAOTLW010000095 DAOTLW010000000
VERSION     DAOTLW010000095.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2798)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2798
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29339_length_2798_cov_8.639446"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="P5516_08730"
     CDS             complement(<1..289)
                     /locus_tag="P5516_08730"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92552.1"
                     /translation="MSSQKQPSITTPPEVGSAQVDLLARLCNAMAVSGDEQEVRQIVL
                     DEIQPYCDQVEVDGLGNILATRLGSQAQKVRVMIAAHMDEVGFMLTNDEGEG"
     gene            complement(292..1350)
                     /locus_tag="P5516_08735"
     CDS             complement(292..1350)
                     /locus_tag="P5516_08735"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HRT92553.1"
                     /translation="MTLDLLSHLKQMLSAPGLSAYEDPIRDIIAEAWRPLTDELSVSR
                     VGSLHALKRGDGAEPRPKVLLSAHMDAIGLMVTAITEGFLHVTQVGGVDARILPGQLV
                     TVHGRKDLPGIVVMPPEFLLPPEVEKGSVPLQYLLVDVGLQPAEVEELVRVGDVVSFA
                     QPPLELTGGAVAGHTLDNRASVAAVTHCLELLQRRKHVWDVWAVASVQEEIGLTGAYT
                     SPFAIQPDFAIALDVTFAKGPGSTDWRTVPLGKGIALAWGPNIHPALYEALTKIADEL
                     DIPYSPEPAPSMTGTDAAAIQIVRAGIPCVLVSTPLRYMHTPVETVVLKDIQRAGRLI
                     AEFISALPVDFMQTLEWK"
     gene            complement(1379..1750)
                     /locus_tag="P5516_08740"
     CDS             complement(1379..1750)
                     /locus_tag="P5516_08740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92554.1"
                     /translation="MDSVSKQKRTRYLLTLLILITIPCYALGIVLLKLNRGPLEPTPT
                     ASATPTITATATITLTPYLTRTATVTPTITLTPTITQTRTPTLTPTPTQTPTLTLSPT
                     LTETPTETLPPTEPPISEETP"
     gene            1901..2023
                     /locus_tag="P5516_08745"
     CDS             1901..2023
                     /locus_tag="P5516_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92555.1"
                     /translation="MASKDKGKKEERKKPKMTIKEKRKVKSEKNKTSTSISTGA"
     gene            2254..2326
                     /locus_tag="P5516_08750"
     tRNA            2254..2326
                     /locus_tag="P5516_08750"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2287..2289,aa:Ala,seq:cgc)
     gene            2464..2536
                     /locus_tag="P5516_08755"
     tRNA            2464..2536
                     /locus_tag="P5516_08755"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2497..2499,aa:Ala,seq:ggc)
BASE COUNT          632 a          743 c          785 g          638 t
ORIGIN      
        1 agccttcgcc ttcatcattg gtcagcataa agccgacttc gtccatgtgc gccgcaatca
       61 tcacgcgcac tttttgcgcc tggctgccca aacgggtagc taatatgttg cctaacccgt
      121 ccacttccac ctgatcacaa taaggctgga tttcgtccaa cacaatctgg cgcacctctt
      181 gctcatctcc agaaactgcc atggcgttgc ataagcgcgc taaaaggtct acttgggctg
      241 agccaacttc cgggggcgtt gtaattgaag gttgcttttg tgagctcatc gctatttcca
      301 ctccaaagtt tgcataaaat ccacgggcag cgcgctgata aactcggcaa tcagcctgcc
      361 ggcgcgctga atatccttga gtaccactgt ttctacgggc gtgtgcatat agcgcagcgg
      421 tgtgctgacc agcacacaag ggatgccagc ccgcacaatc tgaatcgcgg cggcatctgt
      481 gccagtcatg ctgggggctg gttcagggga atagggaatg tccaattcat ctgcgatttt
      541 ggtgagcgct tcgtacagcg ctggatgaat attcggaccc cacgccaagg cgatgccttt
      601 gcccagcgga acggtgcgcc agtctgtgct gcccggacct ttagcaaagg tgacgtccag
      661 cgcaatggca aaatcgggtt gaatggcaaa tggcgaagta taggcacccg tcaagccaat
      721 ttcctcttgc acgctagcca ctgcccacac gtcccataca tgtttgcggc gctgcaggag
      781 ttctaagcaa tgcgtcaccg cggcaaccga agcgcggtta tcgagtgtat gcccagctac
      841 ggcgccgcct gtaagctcca gcggcggctg agcaaaagag accacatctc caacgcgcac
      901 cagctcttcc acttcagcag gctgcaagcc aacatccaca aggagatatt gcagcggcac
      961 agagcctttt tccacttctg gtggcagcaa aaattctggc ggcatcacca cgatcccagg
     1021 taaatctttt cgtccatgca cggtgacgag ctgcccaggc aaaatgcggg catccacccc
     1081 gcccacttgt gttacgtgta aaaagccttc cgtgatagcc gtaaccatca agccgatggc
     1141 gtccatgtgc gccgagagca gcactttagg gcgcggctcg gcgccatcgc cgcgcttgag
     1201 cgcatgcaag ctgcccacgc ggctgacgct cagttcatct gtgagcgggc gccaagcctc
     1261 ggcaataatg tcgcggatcg ggtcttcgta agccgaaagc ccgggtgcgg agagcatttg
     1321 tttgagatga gagagtaaat caagtgtcat caggcttatt acctttcatt tgtagttctt
     1381 agggcgtttc ttctgaaatt ggcggttcgg ttgggggcaa agtctccgtt ggggtttcag
     1441 ttaacgttgg gcttagcgtc aacgtgggtg tttgggtggg tgtaggtgtt agtgtcggcg
     1501 tgcgcgtctg agttatggtt ggcgtcaatg taatcgtcgg ggttaccgtg gcggtgcggg
     1561 tgaggtaagg cgtaagcgtg atcgtggcag tcgcggtgat ggtcggtgtg gcgcttgcgg
     1621 ttggggtcgg ctccagcggt ccgcggttga gcttgagcag cacaatccct aaggcgtagc
     1681 aagggatggt gatcaggatg agcaaagtaa gtaagtaacg tgtccgcttc tgcttcgaaa
     1741 ccgagtccaa ggttatctcc aggctgctta atcataccag agggtaggtt tttcggataa
     1801 tttttaatca taccatcaag ccgagagacc gcatcaattt acgatataat agaggcagta
     1861 cgattgaagc gaccccaagt tacgtcaaag gaggtgccct atggcgagta aggataaagg
     1921 caagaaagaa gagcgcaaga aacctaagat gaccatcaag gaaaagcgca aagtcaagag
     1981 cgaaaagaac aaaacttcca catcgatttc aacgggcgca taaccccagc cactccgcca
     2041 ggcatctcat gaggtgcctg gcagttttta agcccaagct tcagcttgac attcacccct
     2101 catctcgttc ttgcatggta ccctacaggc aggcgttgca ctctcctttc caggcaaggt
     2161 gctgaccccc gcacacatgc ttttcgacct tagcttgaca ttcacccccc actcaggtag
     2221 aatttttttg ccctgaagtt ttgagcgatc gtcgggcctg tagcgcagtt gggagcgcgc
     2281 ttcaatcgca ctgaagaggt cgagggttcg aatccctcca ggtccatcat gagccgggag
     2341 gcacctgatg gacaagacca caggcgccgg ctaggcaacc accgtaataa cgggcccata
     2401 gcgcacgcag cccccttccg ggggattggg agcgcgaagg gcagcacgaa aaacatttaa
     2461 tccgggccca tagcgcagtt gggagcgcgt ctcaatggca ttgagaaggt cgagggttcg
     2521 aatccctctg ggtccatttt tatttttaaa cacattgaga aggtcagccc tttacagggg
     2581 gctgcgcacg gttcgaatcc ccctgggtcc acaggaagag cacctgtaaa ggtgcttttt
     2641 cgttggtaga gaggttcaaa ttgcttgagc tggtggctgc gcacgggttc gaatccccct
     2701 gggtccacaa aaagcatcct tttggatgct tttttattat tttggattga gaaggtcggc
     2761 cctttacagg gggctgcgca cgagttcgaa tccccctg
//
LOCUS       DAOTLW010000096         2781 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_29566_length_2781_cov_10.899486, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000096 DAOTLW010000000
VERSION     DAOTLW010000096.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2781)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2781
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_29566_length_2781_cov_10.899486"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(183..431)
                     /locus_tag="P5516_08760"
     CDS             complement(183..431)
                     /locus_tag="P5516_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92556.1"
                     /translation="MDYSLIGKIEKAKRYAQERERFEFHSFKVVVKGDNNAHVVDYEN
                     GAWKCDCEYFMSRGRCVHTMALESILEGMTLPEPVNAQ"
     gene            complement(606..1073)
                     /locus_tag="P5516_08765"
     CDS             complement(606..1073)
                     /locus_tag="P5516_08765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT92557.1"
                     /translation="MTERPLFYELLPATLGDLSQLNALEKACFPLDAWPLIEQIAALT
                     FPATIRIKAEHKGRLIGFVGGDIRRAHQTGWITTLAVLPEYRRLGIGEALLRAGEQAL
                     RMPLVKLSVRRTNLEAQLLYLKNGYHKVETWRRYYEGGEDAIVMEKDMPAFKL"
     gene            complement(1141..2046)
                     /locus_tag="P5516_08770"
     CDS             complement(1141..2046)
                     /locus_tag="P5516_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HRT92558.1"
                     /translation="MFGEASILIYCLIGAVLILLVVFLAAAIKIVPEYRRIVVFRLGR
                     CIGAKGPGLILLIPFVDRPVTVDLREQVREIPAQQSITQDNAPLSIDFLWYYKVFDPV
                     QSVLQVQNFELAAQGIATTTLRSVIGGILLDGVLSEREKINTALRARLDEVTERWGVK
                     VTNVEIREIVPPRDVQDSMNRQLTAERNRRAVVTESTGSREAAINVAQGEKQANILRA
                     EGEKQATILKSEGERKAQVLKAEGYQQALEAIYAAARNIDQKTMTLQYFETLKTMASG
                     PSTKYIFPMEFTSLLSDFVKKNTEK"
     gene            complement(2082..2684)
                     /locus_tag="P5516_08775"
     CDS             complement(2082..2684)
                     /locus_tag="P5516_08775"
                     /inference="COORDINATES: protein motif:HMM:NF014062.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HRT92559.1"
                     /translation="MNLLLDPNVAYVILVIGFVLGVLALFTPGTGILEVGVLFAIVLA
                     GYAVIKLPINLWALILLMVGVVPFLIAVRKYKHWLYLIPAIASIVVGSIFLFKLESGA
                     PAIDPFLAVFMSVIAIVLLWFVARKGIEAMKLRPSQDLGALIGQVGVARSDIRTSGSI
                     YVGGEEWTARSDKPIREGQYARVIGRDGLVLVVEPAKEEK"
BASE COUNT          652 a          809 c          644 g          676 t
ORIGIN      
        1 ccctctccgc tcttaataac tcatcgctaa gtcaatctgg gttcgaatcc tcatccttac
       61 aggacgctcc gcggccctct ccgccagaga acggcttcca ggacgcgaag accgcagccc
      121 tctctgacaa ttaaaaagac cctggcaata agacaccagg gtcttgattg tattccacca
      181 ggttattggg cgttcaccgg ttccggaagt gtcatccctt cgaggatact ttcaagcgcc
      241 atcgtatgca cacagcgccc gcgcgacata aagtattcac aatcgcattt ccaggcgcca
      301 ttctcgtaat ccacaacatg ggcattattg tcacccttga ccaccacctt gaaagaatgg
      361 aattcaaacc gttcgcgttc ctgcgcgtag cgttttgctt tttcgatctt gccaatcaga
      421 ctgtaatcca tttctattag ctccttttca tcaatatggt agcaaaaagg cacgcttgaa
      481 caggcgtgcc ttcagttgtt ttctccatta tggtaaccca atactcgcat ggtgatcagt
      541 tctccactta ctttatgtta gacccattca cgttctttgt caactacgat tgcctgacaa
      601 ttgccctaca gtttgaacgc gggcatatct ttttccatca ctatggcgtc ttcgccgcct
      661 tcgtaatacc ttcgccatgt ttccactttg tgataaccgt ttttcaaata aagcaattga
      721 gcttccaggt tcgtgcggcg cacagaaagt ttcaccagcg gcattctaag cgcctgctcg
      781 ccggcgcgca gcaaggcttc tccgataccc agccgccggt attccggcag caccgccagc
      841 gtggtgatcc accctgtttg atgcgcacgc cggatatcac caccgacgaa cccgatcaac
      901 cttcccttgt gctccgcttt gatgcgtatc gtggctggaa aggtgagcgc agcaatttgc
      961 tcaatcagcg gccaggcgtc cagtggaaag caggcttttt ccagcgcgtt taactgggaa
     1021 aggtctccca gcgttgcggg tagtagttca tagaacaatg gtctttcagt catcgcagcg
     1081 taagaaatgt gggttgggaa aaactctccc aacccacaac ttactcataa tcatccgtcc
     1141 ttacttctcg gtgttcttct tgacgaagtc gctcaacagg ctggtaaatt ccattgggaa
     1201 gatatacttg gtggaaggcc cgctcgccat ggtcttgagg gtttcaaagt actgcagcgt
     1261 catggtcttc tggtcaatat ttcgcgcagc ggcgtaaatc gcttccagcg cctgctggta
     1321 cccttccgcc ttcagcacct gggccttgcg ttcgccttct gatttgagaa tcgtcgcctg
     1381 tttttcacct tcagcgcgca agatattggc ctgtttttca ccctgcgcca cgttgatcgc
     1441 agcttcgcgg ctacccgttg attcggtgac cacagcgcgg cggtttcgtt cagcagtcaa
     1501 ctggcggttc atcgaatcct gcacgtcacg cggcggcacg atctcacgga tttccacgtt
     1561 ggtcactttc accccccaac gctcggtcac ctcgtccagg cgggcgcgca gggcggtgtt
     1621 gatcttttca cgttcagaca gcacaccatc caacaggata ccgccaatga cggaacgcag
     1681 cgtggtggtg gcaatacctt gtgccgccag ttcaaaattc tgcacctgga gcacagattg
     1741 cacggggtca aacactttgt aataccaaag aaaatcaatg gacaacgggg cgttatcctg
     1801 cgtgatggat tgctgcgccg gtatctctct tacctgctcg cgcaggtcta cggttaccgg
     1861 gcgatcaaca aagggaataa gcaagatcaa cccgggccct ttcgcgccaa tacagcgccc
     1921 cagcctgaag accacgatcc tgcgatattc aggaacgatc ttgatggccg cggctaaaaa
     1981 gaccaccaac agaatgagca ccgccccgat caaacagtaa atcagaatac tcgcttctcc
     2041 gaacatagct cctccttcag tttagataaa acgaaaatgt attacttttc ttctttagcc
     2101 ggttcaacta ccaacaccaa tccatcgcga ccaatgactc tggcatattg tccttcgcgg
     2161 atcggtttat cactgcgcgc ggtccattcc tccccgccta cataaatgga gccgctggtg
     2221 cgaatgtcac tgcgcgcgac gcctacctgc ccgatgagcg cgcccaggtc ttgcgagggt
     2281 cgcagtttca tggcttcgat gcccttgcgc gcgacaaacc agagcagcac aatggcgatt
     2341 acgctcataa ataccgccag gaatgggtcg atcgccggag cgccggattc caatttaaac
     2401 aggaatattg aaccgacaac gatagaagcg atagcgggga ttaagtacaa ccagtgctta
     2461 tacttgcgca cggcgatgag aaaggggacc acgcccacca tcagcaggat gagcgcccac
     2521 aggttgatgg gcagtttgat gaccgcgtac ccggccagga cgatggcaaa cagcacaccg
     2581 acctctaaaa taccggtgcc gggtgtgaac aacgccagca cgcctaaaac gaatccgatc
     2641 accaggatga cataagccac atttggatca agcagcaggt tcatactatc ctttcatttt
     2701 cctgaattca ttatacacga ctatgtttct catttcacag gtttctatcg caaacccaat
     2761 tgtccagcat gattactgta g
//
LOCUS       DAOTLW010000097         2457 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_34648_length_2457_cov_7.589925, whole genome shotgun sequence.
ACCESSION   DAOTLW010000097 DAOTLW010000000
VERSION     DAOTLW010000097.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34648_length_2457_cov_7.589925"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..352)
                     /locus_tag="P5516_08780"
     CDS             complement(<1..352)
                     /locus_tag="P5516_08780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92560.1"
                     /translation="MVELNKQTFELNKPIGYVVGGGLEANLQVRLTVPPQEVQEGAFV
                     VIDSGTWRFYGLVTDLQLGSTDPRFADEQSELRLPAGLAGLLHGQTLFTNLNVMPVLM
                     IDRGPEPGSLSYEAW"
     gene            complement(360..968)
                     /locus_tag="P5516_08785"
     CDS             complement(360..968)
                     /locus_tag="P5516_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92561.1"
                     /translation="MSEETKIPIGKLLRADSRQFVAGCRVKELETPALGALCKVKMED
                     GSDIFGLITNIQINDDGLVKQLVSGLSIPSEYTADNRYNRNLPVEIYVLAVGFQRAGR
                     LYHRLPPRPPLSLDALYLCDDAELVRFTNSGGYGYFRHILRLEDQPVEELLVAHLAQA
                     RAAHELQGNRDWYGGACQELITLLRDDYPRLMNVLNALAELE"
     gene            complement(977..2134)
                     /locus_tag="P5516_08790"
     CDS             complement(977..2134)
                     /locus_tag="P5516_08790"
                     /inference="COORDINATES: protein motif:HMM:NF020933.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HRT92562.1"
                     /translation="MPVNYHQIESHLPEYGKRMKEFETALAQAEQTLWEQFTHLDEDV
                     AALRDKIEKAEVQLRRLYCAKPVSEPLLTHKAAPLPPAGYTLIAADGSQIAPNRHRPL
                     QFGVINVGLIQARLGSGNPPEVSIQSTLLDPEELLSPEGSLIGEDEVALYRDFAERRA
                     LLEVALAAQAPVIALTDGLLDIYQQYSAPQLNFQAEMRTIHQQLEARGVISAGYIDKP
                     GAEMLSRMLDLLQTPEMDLVAYDDQKRSLRGISDARLLAKLLTMPGERSAVFETVSKG
                     AAQSSLKVHFFYINISLGGMPYLARVEFPAWLSAQPELVDLMHAVIFQEVQVLDRHPY
                     PYLLHRAHELAVITMQEHEQVEAMLLQEYARQGLHAGLHSNKEANKQLLKF"
BASE COUNT          539 a          693 c          651 g          574 t
ORIGIN      
        1 gccaagcttc gtagcttagg ctgcccggtt cgggtccgcg gtcaatcatg aggactggca
       61 tcacgttcaa gttggtgaaa agcgtctgcc cgtgcagtaa acccgctaac cccgcgggca
      121 agcgcagctc gctttgctca tcggcaaaac gcgggtcagt cgagcctaac tgcaggtcgg
      181 tgaccagccc gtaaaagcgc caggtgccgc tatcaatgac cacaaaagcg ccttcctgca
      241 cttcctgcgg cggcaccgtc aggcgcacct gcaggttggc ttccagcccg ccgcctacaa
      301 cataaccgat gggtttattg agctcaaatg tttgtttgtt cagttctacc atggctccct
      361 cactctaatt ctgctaaggc attgagcaca ttcatcaagc gcgggtagtc atcgcgcaat
      421 aaggtaatca gctcctggca ggcaccgcca taccaatcgc ggttaccttg cagctcgtgg
      481 gcggctcggg cttgggctaa gtgtgccacc agcagttctt cgaccggttg atcttccaac
      541 cgcagaatgt gccggaagta accataaccg ccgctattcg tgaagcgcac cagttccgca
      601 tcatcgcata agtaaagcgc atccaggctg aggggcgggc gtggcggcag gcggtggtaa
      661 agcctgccag cgcgctgaaa gcccaccgcc aaaacataaa tctccactgg caggttgcgg
      721 ttataacggt tatccgcagt gtattcactg ggaatgctga gcccagaaac cagctgtttg
      781 accaagccgt catcgttaat ttgaatattg gttatcaacc cgaagatatc ggagccatct
      841 tccatcttta ccttacatag tgcccccaaa gcaggcgttt ccagctcctt cacgcggcag
      901 cccgccacaa attggcggct atcggcgcgc agcagctttc caatgggaat tttagtttct
      961 tcgctcatct cgtccctcag aattttagca attgtttgtt ggcctcttta ttcgaatgca
     1021 agccggcatg taagccctgg cgcgcatatt cctgcaaaag catggcttcc acttgttcgt
     1081 gttcctgcat ggttattact gccagctcat gcgcgcggtg cagcaggtag gggtaaggat
     1141 gccggtccag cacctgcact tcctgaaaaa tgactgcgtg catgaggtcc actaattcag
     1201 gctgggcact aagccaagcc gggaattcca cccgggctaa gtaaggcatg ccacccaagc
     1261 tgatattaat atagaagaag tgcaccttca ggctggattg ggctgcgcct ttgcttacgg
     1321 tctcgaacac cgctgagcgc tctccaggca tagtgagcag tttagccagc aggcgggcat
     1381 cgctaatgcc ccgcaggctg cgtttttgat catcgtaagc gaccaaatcc atctccggcg
     1441 tttgcaggag gtctagcata cggctgagca tttctgcacc gggcttgtca atataaccgg
     1501 cgcttataac gccgcgcgct tctaattgtt ggtgaatcgt gcgcatctct gcttgaaaat
     1561 taagttgcgg agcagaatat tgctggtaaa tatccagtaa cccgtcggta agtgcaatca
     1621 cgggcgcttg agccgccaga gctacctcca aaagcgcacg ccgctcagca aagtcgcggt
     1681 agagtgccac ctcatcctcc ccaatcaggc tgccttcagg gctgaggagc tcttccgggt
     1741 cgagcaatgt gctctggatg ctgacctccg gcgggttgcc gctgcctaaa cgcgcctgaa
     1801 tcaaacccac attgataacc ccaaactgga gcgggcggtg gcggttgggc gctatctgcg
     1861 agccatcggc tgcgataagt gtatagcctg ccggcggcag tggtgcggct ttatgggtca
     1921 gcaatggctc agagaccggc ttggcgcaat acagccggcg tagctggact tccgcttttt
     1981 cgatcttgtc acgcagcgca gcgacgtctt catccaggtg agtgaactgc tcccagaggg
     2041 tttgctctgc ttgcgccaac gcagtctcga attccttcat gcgcttgcca tattcgggta
     2101 aatggctttc aatttgatga taattaactg gcatgatccc ttatgagagg ttatgaactg
     2161 aaccccgcag gtaattataa ccaatgcgca ctggctttgc ccgccaaatt agagtaacga
     2221 tttgggttgc agccacgtcc cgcaaaacca atctcagatt gcgccggtct cttgagtgcg
     2281 tagcctgccc gtagggtgcc gagcgcgggg gacccgcgga ttgcgccggt ctcctgaccg
     2341 agcgcacaag acgaccgtta ggtctcctga gaaatacacc atataattaa cacatggatc
     2401 gttttaaaat tgtagacgga gtttatgtct attttgtcac cttcaccatc atcgatt
//
LOCUS       DAOTLW010000098         2456 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_34662_length_2456_cov_10.905873, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000098 DAOTLW010000000
VERSION     DAOTLW010000098.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2456)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2456
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_34662_length_2456_cov_10.905873"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..2417)
                     /gene="cas3"
                     /locus_tag="P5516_08795"
     CDS             complement(<1..2417)
                     /gene="cas3"
                     /locus_tag="P5516_08795"
                     /inference="COORDINATES: protein motif:HMM:TIGR01587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated helicase Cas3'"
                     /protein_id="HRT92563.1"
                     /translation="MFDPRALLADAWGIWYIDREPTTVVLRFSQRVANRVKESLWHPS
                     QKLVDQDDGSTIWQATIAEPQEMLPWIRGWGADCEVLEPPELRAKLEKEVQRLARVYG
                     VGEIKREVDLVAHWRKSDNEAQLLWEHLQETAEIAAKLSTKIGLPEIGRIIGLLHDFG
                     KASKEYQDYLRTQEGKISPDEDGYSEAKRGEVDHSTAGAQLIYEKLASRGQEGKFLAQ
                     FLSLIIASHHSGLIDCLKPDGFNEFERRMNKDKDLTHLAEAKGKLPDIEKQLDEILSQ
                     PIEKLFFERLMGMRDSDKDVTLPFKHGLLARFLLSCVLEADRLNTADFENPGNEEVRN
                     YGKYVPWEILIERLEAKYAEYAQETAQKSGRALEVNQLRAQVAQACLEAAKKPKGIYQ
                     LTVPTGGGKTEASLRFALHHAQKHKMDRVFYIVPYITIIEQNADKVREILEKPEERDK
                     VVLEHHSNLTPEQETRRHNLLAENWDAPIVFTTQVQFLEALFGSGTRDARRMHQLANS
                     VIILDEVQTIPIKIVHMLNAALRFLTHDCGATVVLCTATQPPLDKLPENPYRALTIEE
                     EKKIIRNEAELFQKLKRVEVHDERKPGGLTNAEIADLAQKALEEKGSVLIVVNTRASA
                     QALYQEIKSRNLGAALYHLSTNMCPAHRKDKLDKIKANLEAKEPVICVSTQLIEAGVD
                     IDFGAVIRALAGLDSIAQSAGRCNRHGLRKDGGSLWVVNPREENLDKLEEIKIGREHA
                     QRVLDDFSMTPDKFDNDRIGLNAIAAYYNFYYQARKDQMRYPVNANSSAGRNDDLFNL
                     LSMNELSV"
BASE COUNT          497 a          674 c          605 g          680 t
ORIGIN      
        1 actgaaagtt cattcatcga aagcaggttg aacaaatcat catttctgcc tgcggatgaa
       61 ttcgcgttca caggatagcg catctggtct ttccgcgcct gataataaaa attgtaatat
      121 gcagcgatgg cgttcaggcc aattcggtcg ttgtcaaatt tgtcaggtgt catgctaaaa
      181 tcatcgagaa ccctttgagc atgctcacgc ccgattttga tttcttccag tttgtccaga
      241 ttctcctccc gcggatttac cacccacaaa ctcccgccgt ctttgcgcag tccgtgacgg
      301 ttgcagcgcc ccgccgattg ggcaatcgaa tccaatcccg ccagggcgcg gatgaccgcg
      361 ccgaagtcaa tatccacgcc cgcttcgatg agttgcgtgc tgacgcaaat cacgggttct
      421 ttggcttcca ggttggcttt gattttatcc agcttatcct tgcggtgcgc gggacacata
      481 ttcgtgctta gatggtagag cgccgcgccc agattgcggc tcttgatttc ctgatataac
      541 gcctgcgctg aagcgcgtgt attgaccaca atcagcacgc tgcctttttc ctccagcgct
      601 ttttgggcaa ggtcggcaat ttcggcgttg gtcaagccgc cgggcttgcg ctcgtcatgc
      661 acctccaccc gtttcagttt ctggaacaat tccgcttcgt tccgaatgat ttttttctct
      721 tcttcgatgg tcaaagcgcg gtaggggttt tcgggcagtt tatccagcgg cggctgggtg
      781 gcggtgcaaa gcacaacggt cgccccgcag tcgtgggtca ggaagcgcag cgccgcgttc
      841 agcatgtgaa cgattttgat gggaatggtc tgcacttcgt ccaggatgat gaccgagttc
      901 gccagttggt gcattcggcg ggcgtcgcgc gtgcctgaac cgaacaacgc ttccaaaaac
      961 tgaacctgcg tggtgaagac aatcggcgcg tcccagtttt ccgccagcag gttgtggcgg
     1021 cgcgtctcct gttcaggcgt caggttggag tgatgttcga gaacgacctt atcgcgctct
     1081 tctggttttt ccagaatttc gcgcactttg tctgcgttct gttcaatgat agtgatatag
     1141 gggacaatat aaaagacccg atccatctta tgcttctgcg cgtgatgcag ggcgaagcgc
     1201 agactcgcct ctgtctttcc gcctcccgtc gggacggtca actggtaaat gcctttgggc
     1261 tttttcgccg cctccaggca ggcttgcgcc acctgcgccc gcagttgatt gacctccaat
     1321 gcccgccctg atttttgcgc cgtttcctgt gcatactccg catactttgc ctccagccgt
     1381 tcgatgagaa tctcccaggg aacatacttc ccgtaattgc ggacttcctc gttaccaggg
     1441 ttctcgaaat cggcggtgtt caggcggtcg gcttccagca cacagctgag cagaaaacgc
     1501 gccagcaaac catgtttgaa gggcaaagtg acgtccttat cagagtctct catgcccatt
     1561 agtctttcaa aaaaaagttt ttcaatcggc tgcgagagaa tttcgtccag ttgtttttcg
     1621 atgtcgggca gtttgccttt ggcttcagcg aggtgcgtca ggtctttgtc tttgttcatc
     1681 cgccgctcga actcattgaa gccatcgggc tttaagcaat ctattagtcc cgaatgatgt
     1741 gaagcgatta tcagagacaa aaactgcgcc aggaatttac cctcctgtcc ccggctggcg
     1801 agtttttcgt agatcaattg cgccccagcg gtagagtggt caacttcgcc gcgcttggct
     1861 tctgaatacc cgtcttcgtc agggctaatc tttccctctt gtgtccgcaa ataatcttga
     1921 tattccttgc ttgcttttcc aaaatcatgt agcaagccga ttattctacc aatctctgga
     1981 agtccaattt tggttgatag ttttgcggcg atttcagccg tctcttgtag atgctcccat
     2041 aacaattggg cttcattatc actttttcgc caatgagcaa caaggtcaac ttcccgtttg
     2101 atttcaccca ccccatacac ccgtgcgagt cgctgcactt ccttttctag cttagcccgt
     2161 aattccgggg gttccaacac ctcacagtcc gcgccccagc cgcggatcca gggaagcatt
     2221 tcctgcggtt cggcaatagt cgcttgccag atggttgatc catcatcttg atccactaat
     2281 ttttgggatg gatgccagag actttcctta acccgattcg caacccgctg actaaaacgt
     2341 aacacaacag ttgtaggttc tctatctatg taccatatac cccaggcgtc agcgagtaag
     2401 gcgcggggat caaaaatttc tgggatttta taggaatcat tagttttttc tgcccg
//
LOCUS       DAOTLW010000099         2379 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_36136_length_2379_cov_5.622203, whole genome shotgun sequence.
ACCESSION   DAOTLW010000099 DAOTLW010000000
VERSION     DAOTLW010000099.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2379)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2379
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36136_length_2379_cov_5.622203"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..936
                     /locus_tag="P5516_08800"
     CDS             <1..936
                     /locus_tag="P5516_08800"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT92564.1"
                     /translation="YNSQQSLTPLVQRLHATVPALCSDYEILLVNDDSRDESWRVITE
                     LCAASPRVIGINLMRNFGQHNALLCGIRQARYELILTMDDDLQHPPEEAPKLFAKLAE
                     GYDVVYGAPAAEKHGFWRDLSSRLIKQVLHKLMGINSAATVSAFRLFRAELAEAFSDY
                     QGSFVSIDVLLSWGTTRFASVTVNHQEREFGKSNYSFRKLMNHTLTLVTGFSNAPLRI
                     ASITGFFFTAFGFGVLLYVLISYFVRGGSLPGFPFLASIISIFSGVQLFALGIFGEYL
                     GRMFSRTLNKPVYTIREVLRSEPLPENQENPSGER"
     gene            926..1675
                     /locus_tag="P5516_08805"
     CDS             926..1675
                     /locus_tag="P5516_08805"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HRT92565.1"
                     /translation="MSAETAPDQATELSPLDETRFGVRSARARLVTTKNLPEVLAFCA
                     ANQVEFLVARCPTTDISAAHAMEAAGFRLMETLLYLRFDLKTRPIPMNKSNVLIRPVQ
                     PTEVSRVGALAHTAFTNYYGHYHADPKLDPQKSTEVYVDWAERCCTDSAAANLVLVAE
                     AEGELVGFRALRVNSPIQGEFILAGVSPEFRRRGIYHAFIVEGLKWCREQGLEEVLNS
                     THVANVAVQRACLHAGFEPAYSWYTFHKWFH"
     gene            1754..>2379
                     /locus_tag="P5516_08810"
     CDS             1754..>2379
                     /locus_tag="P5516_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HRT92566.1"
                     /translation="MSKIPFNKPPLLGNEFDYIREAIASGHISGDGAFTKRCHTILEQ
                     LIGAPKVLLTTSCTHALEMAALLLDIQPGDEVIFPSFTFVSTVNAFVLRGAVPVFADI
                     HPDTLNLDESQLERLVSAKTRAVVPVHYAGVGCEMDTILAFANEHNLAVVEDNAHGLT
                     GAYKGKPLGSFGCLATQSFHETKNFSCGEGGALVINEPQYIERAEIIR"
BASE COUNT          537 a          659 c          600 g          583 t
ORIGIN      
        1 tacaacagcc agcagagcct gacaccgttg gtccaaagac ttcatgccac tgtgcccgcc
       61 ttatgctccg attatgaaat cttgctcgtt aacgatgata gccgagatga aagctggcgc
      121 gtgattacag agctgtgtgc cgccagcccg cgcgtcatcg gcatcaacct gatgcgtaac
      181 tttggtcaac acaacgccct tctgtgcggc atccgtcaag cgcgttacga actgatcctc
      241 accatggatg acgatttgca gcatccaccg gaggaagcgc ctaagctctt tgcaaaactc
      301 gcagaagggt atgacgttgt ctacggcgct cccgctgccg aaaaacatgg cttttggcgg
      361 gatttatctt cccgtctgat caagcaggtg ctgcacaagc tcatgggcat caacagcgca
      421 gccacggtat ctgcgttccg gctttttcgc gccgagctgg cagaagcttt ttccgattat
      481 caaggctctt ttgtttccat tgacgtgctg ctcagctggg ggacgacgcg ttttgcttcc
      541 gtgaccgtga accatcagga acgagaattc ggaaaatcca attacagctt tcggaagctg
      601 atgaatcaca ccctgacgct tgtaacgggc tttagcaacg caccactacg gattgccagc
      661 attactggtt tcttcttcac cgcctttggg tttggcgtct tgctctatgt gctcatcagc
      721 tactttgtgc gtggtggcag cttacccgga tttccttttc tagcttctat catttccatt
      781 ttttcaggtg tgcagctttt tgccctgggt atttttggcg aatacctagg caggatgttc
      841 agccgtacac tcaataaacc tgtttatacc atccgcgaag tactcaggag cgaacctctt
      901 cctgagaacc aggagaaccc cagcggtgag cgctgaaaca gcacctgacc aagcaacgga
      961 actttctccg ctagatgaaa cccgctttgg ggtcaggtcc gcacgcgctc gcttggtaac
     1021 caccaaaaat ctacccgagg ttttagcgtt ttgcgccgcc aatcaagtgg aattcctggt
     1081 tgcccgctgc cccaccaccg atatctccgc tgcgcatgct atggaagcag ccggcttccg
     1141 cttaatggaa accttgctct acctgcgctt tgacctgaaa acccgtccta tcccgatgaa
     1201 caaaagcaat gtgctcattc gcccggtgca gcccaccgag gtaagccggg tcggcgcgct
     1261 tgcccataca gctttcacaa actactacgg tcattaccat gccgacccaa aactggaccc
     1321 tcagaaaagc acggaggtgt acgttgactg ggctgagcgt tgctgcactg attcagctgc
     1381 cgctaacctg gttttagtgg cggaggcaga aggcgagctg gtgggctttc gcgcgctgcg
     1441 tgtcaattcg cccattcaag gggagttcat cttagcaggg gtgagccctg agttccgccg
     1501 gcggggtatt taccacgcct ttatcgtgga aggtttaaag tggtgccgcg agcagggctt
     1561 ggaagaagtc cttaattcca cgcatgtggc aaacgtggca gttcaacggg cttgtttgca
     1621 tgcgggcttt gaaccagcct attcctggta cactttccat aagtggtttc actgagccga
     1681 tgaggcaagt ctttatatgg cgaatttcac acagaaacct atctcgaaaa tatattacta
     1741 ataagaggcc cccatgagca aaattccctt caacaaacct cctttacttg gaaacgaatt
     1801 cgactatata cgcgaagcaa tcgccagcgg gcatatctcc ggagatggtg catttaccaa
     1861 acgctgccat accatactgg aacagctcat tggtgccccc aaggtgctgc ttaccacatc
     1921 ctgcacgcat gcattggaaa tggctgccct cctgctggat atacaacccg gcgacgaggt
     1981 catttttcca tcattcacct ttgtttccac cgtgaacgcc tttgtgctgc gcggtgcggt
     2041 accggttttt gccgatatcc atccagatac gctcaacctg gatgaaagcc agctggagcg
     2101 cttggtcagt gcaaaaacgc gtgccgtagt gcccgtgcac tatgccggcg tgggctgtga
     2161 gatggatact atccttgctt ttgcaaatga acacaactta gccgtggtgg aagataatgc
     2221 gcacgggctg accggagcct acaagggcaa acctttgggc agctttggct gcctggcgac
     2281 acaaagcttt cacgagacca agaatttttc ctgcggcgaa ggtggcgcac tggtcatcaa
     2341 tgaaccgcaa tacattgagc gcgctgaaat tatccgcga
//
LOCUS       DAOTLW010000100         2353 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_36628_length_2353_cov_8.425587, whole genome shotgun sequence.
ACCESSION   DAOTLW010000100 DAOTLW010000000
VERSION     DAOTLW010000100.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36628_length_2353_cov_8.425587"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="P5516_08815"
     CDS             <1..410
                     /locus_tag="P5516_08815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92567.1"
                     /translation="VRSVGEHPKAADTLGINVIRTRYMSVLLGGMVAGMAGAFFTLGS
                     VGRFEEGMTAGKGFIGLAAMIFGNYTPIGALGAGILFGFADSLGSKLSLLGSAIPSTL
                     MSTAPYVITMIVLAGFVGKGHVPAADGVPYTKE"
     gene            503..1411
                     /locus_tag="P5516_08820"
     CDS             503..1411
                     /locus_tag="P5516_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT92568.1"
                     /translation="MMPNIIVDRISKAFGNLQAVKNVSFEVNPGEIFGLLGPNGAGKT
                     TSIRIILDIFKADSGTVSILGGAMTEEKKNRIGYLPEERGLYQDIQLERCLVYLATLK
                     GLEESEARGRINDYLQKFDLADARKKKVKELSKGMQQKAQLITTLVHKPEVVIIDEPF
                     SALDPVNTQMVKDLLKEERHRGTTIVMCTHQMHQVEELADRIALINAGETVLYGGLTE
                     IRRRFASDGVLIRAVNPLPSAIPGVQEVRPHNSNTLLKLESGKTPQEVLNTLIHQGVI
                     LEHFEIALPSLDEIFIQVVNHESERK"
     gene            1408..>2353
                     /locus_tag="P5516_08825"
     CDS             1408..>2353
                     /locus_tag="P5516_08825"
                     /inference="COORDINATES: protein motif:HMM:NF024110.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92569.1"
                     /translation="MNKIWLVFRNEYSTHVKKKGFIFGILSMPFFVVLMVLVGFISVW
                     LEHNPAPVGFIDPNHVIGNAQQVPLKKKKPFPPVRSLPFEDESSAEQALRDGSIQAYF
                     LLTDNYLNSGEVIMVTTEKTGSNAERDFGDFLVHNLVKDQPPVLAERLREGPNLIIRS
                     LDGVREIAADDWVSILMPILAGVLFVIAVNISGNYLLQAVVAEKENRTMEIIVTSISP
                     GQLMAGKVLGNLLVGLTQLFTWFIFAIIALKLLPTVFPVKFAPRVDPAGILLIAATFL
                     PAFVMVAAFMGAIGATATDPREAQQITGWFTIPIVIPLW"
BASE COUNT          566 a          624 c          616 g          547 t
ORIGIN      
        1 gcgtgcgcag tgtgggcgag catcccaagg cagccgatac actgggcatc aacgtcatca
       61 gaacccgcta catgtcggta ttgctgggcg ggatggtggc tgggatggct ggagcgttct
      121 tcactctggg gtctgtcggg cgcttcgagg aaggcatgac cgcggggaaa ggctttatcg
      181 gcctggccgc catgatcttt ggtaactaca cccccatcgg agcgcttgga gcgggcatcc
      241 tttttggttt tgccgattct ttgggctcca agctatcttt actggggagc gccatcccct
      301 caacattgat gtccaccgcg ccctatgtga tcaccatgat cgttctcgcc gggtttgtcg
      361 gcaaggggca cgtccccgcg gcggatgggg tgccgtacac caaagaataa gcttgataac
      421 tcagaaacct gcaccggtct tccatcgtat attataagga agaccggttt tgttataatc
      481 accacaataa acagaaaggt acatgatgcc caatattatc gtcgaccgca tttccaaggc
      541 ttttggcaat ctccaggcgg taaaaaacgt ctctttcgaa gtcaatcctg gcgaaatctt
      601 tggtctgctc ggaccaaacg gcgcaggcaa gaccacctcc atccgtatca tactggatat
      661 ttttaaagca gactccggaa ctgtgagtat cctgggcggc gccatgaccg aagaaaagaa
      721 aaatcgtatc ggttatctgc ctgaagaacg cgggctttac caggatatcc agctggaacg
      781 ctgtctcgtt tatcttgcca ctctgaaagg gctcgaagaa agcgaggcgc ggggacgcat
      841 caatgactat ctgcaaaagt tcgacctggc tgatgcgcgc aagaaaaagg ttaaagaact
      901 cagtaaaggt atgcagcaaa aagctcagct gatcaccacg ctggtgcaca aacctgaagt
      961 ggtgatcatc gatgaaccct tcagcgcgct cgacccggtg aacacgcaga tggtgaaaga
     1021 cctgctcaaa gaagaacgcc accgcggtac gaccatcgtc atgtgcaccc accagatgca
     1081 ccaggtggaa gaactggcgg accgtatcgc gctgatcaat gcgggagaaa ctgtcttata
     1141 tggcgggttg acggaaattc gtcgccggtt cgcttctgat ggcgtgctca ttcgcgcggt
     1201 aaacccgctg ccatctgcca tccccggtgt gcaggaggtt cgcccgcaca acagcaacac
     1261 tttattaaaa ctcgagagcg gaaaaacccc gcaggaagta ttgaacaccc tcatccacca
     1321 gggggtcatc ctcgagcatt ttgaaattgc tctgccctca ctggatgaaa tatttatcca
     1381 ggtggtcaac catgaaagcg aacgaaaatg aacaaaattt ggctggtgtt tcgcaatgaa
     1441 tactccacgc atgtaaagaa aaaagggttc attttcggca tcctcagcat gccatttttt
     1501 gtggtgctga tggttctggt gggctttatt tcagtctggc tggaacataa cccggctcct
     1561 gttggcttca ttgatccaaa ccatgtcata ggcaacgccc agcaggtgcc gctgaagaaa
     1621 aagaaaccat tcccgcccgt tcgttcgctt ccctttgaag atgaatccag cgccgagcaa
     1681 gcgctgcgcg atggttcaat tcaggcttac ttccttctta ctgacaatta ccttaacagt
     1741 ggtgaagtaa ttatggtcac caccgagaaa accgggtcta acgctgagcg tgacttcggc
     1801 gacttcctgg tgcataacct ggttaaagat cagccgccgg tactcgctga acgtttacgc
     1861 gaaggaccaa acctcatcat ccgttcgctg gatggcgtgc gggaaatagc cgccgatgat
     1921 tgggtttcca tcctcatgcc catactggcg ggggttctct ttgttatcgc ggtcaacatc
     1981 agcgggaact acctgctgca ggcagtggtg gcggaaaaag aaaaccgcac catggagatc
     2041 atcgtgacct ccatatcgcc gggtcaactc atggcaggta aagtgctggg caacctgctg
     2101 gttggcctga cccaactgtt cacctggttc attttcgcca ttatcgcgtt gaagttattg
     2161 cccactgttt tcccggtaaa attcgcgccg cgggtggatc ctgcaggcat actgctcatc
     2221 gctgccacct ttttaccagc tttcgtgatg gttgcagcat tcatgggagc gataggcgca
     2281 acggcaacgg acccccggga agcgcaacaa ataaccggtt ggttcaccat tcccatcgtt
     2341 atcccgttat ggt
//
LOCUS       DAOTLW010000101         2341 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_36908_length_2341_cov_12.048556, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000101 DAOTLW010000000
VERSION     DAOTLW010000101.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2341)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2341
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_36908_length_2341_cov_12.048556"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            136..1419
                     /locus_tag="P5516_08830"
     CDS             136..1419
                     /locus_tag="P5516_08830"
                     /inference="COORDINATES: protein motif:HMM:NF014684.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92570.1"
                     /translation="MGKKDLEKQDIGKTILEELTGGEPKEKRQKRGIWKVLQIPALAI
                     LTGLIIGAVLIMATSFDVYDAFKVSLWKGLGTAFAEVGEAYLALITGSLGNPVEIVQA
                     LLYGDALDFRNAINPILESLVQSTPYIFAGLACALGFRAGLFNIGVEGQLFIGAAAAT
                     FVGYSLTGLPPYIHMPLAFLAGALGGALWGMVPGLLKATTGGHEVINCIMMNYIAYRF
                     TTFLLTGPMSRPGTGGMPVSPIIEKSAQIPQFFKTPIRFHLGFFIALAFAALVWWVLF
                     KTTWGLNLRTVGTNPRAAKYAGMNIIATTIIGMAASGALAGMAGANEVLAVNRSMAIG
                     LSAGYGFDSIALALLGNSHPVGVIFAALLFGVLKNGATKMMVVSTIPIDIVTILQAVI
                     LIFVAAPAIIRSVYRLKQPKVEAAAIMLSGWGGVK"
     gene            1419..>2341
                     /locus_tag="P5516_08835"
     CDS             1419..>2341
                     /locus_tag="P5516_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92571.1"
                     /translation="MAATSTSIQRVRISDISPARKISTGVVEVLVALFIFAVFARTVS
                     AGSITTFVMTPGGITTGQMGNWVIPSQLTLYLLAALSLVIGLFQLIRGFGKITNLMVG
                     LVFLFFIFSFLTWQAADKSLNLAGMLSSAVQLAVPITMGSFSGILCERSGVVNIAIEG
                     MMLMSAMVGALMGSLTHSAWLGLFFGVLSAVLLAALHAVLSIKYKIDQIISGTVINMF
                     SAGMTAFLSQKFLQTRQELNNPAMFTRIEIPGLAEIPLIGPILFNTNFFVYLMFALLI
                     ILQVALFSTRWGLRVRSVGEHPKAADTLGINV"
BASE COUNT          475 a          645 c          610 g          611 t
ORIGIN      
        1 attatcgccc tccttgaagc cgaggaagta gccaaagaaa ctttagggtt gttgatggcc
       61 ggtatcgtcc ctgacaaaat tgaaaaaccg cagggacaga gggttgtaga aaccactttt
      121 taacgaggtg catccttggg taaaaaagac ttagagaaac aagatatcgg caagaccatt
      181 ctggaagaac tgacaggtgg agaaccaaaa gaaaagcgcc agaagcgcgg tatctggaag
      241 gtactgcaga tccctgcgct ggctatcctg actggtttga tcattggcgc ggttctcatc
      301 atggcgacca gcttcgatgt ctatgacgcg ttcaaagtca gcctgtggaa aggcctgggt
      361 accgcgttcg cagaggtggg agaagcttac ctcgcgctca tcaccggttc actgggaaac
      421 ccggtggaga ttgtccaagc cctcctctac ggcgacgcgc tggatttcag aaacgccatc
      481 aatccgattc ttgaaagcct ggttcagagc acgccttaca tcttcgccgg actggcctgc
      541 gccttagggt tcagagccgg cctgttcaat attggcgttg aagggcagtt attcatcggc
      601 gctgccgcgg ctacctttgt aggttactca ctcaccggtc taccgcctta tatccacatg
      661 ccgttggctt tcctcgctgg agcattgggc ggcgctctct gggggatggt ccccgggttg
      721 ctcaaagcca ccaccggcgg tcacgaagtc atcaactgta ttatgatgaa ctatatcgcc
      781 tatcggttca ccaccttcct gctcactggc cccatgtctc gcccgggcac cggtggtatg
      841 cccgtaagcc cgataatcga gaaatccgcc cagattcccc agttcttcaa gacgcctatc
      901 cgtttccacc tggggttctt cattgcgctg gcattcgccg cgctggtttg gtgggtgttg
      961 tttaaaacaa cctggggatt gaacctgcgc acggtgggca ccaatccgcg cgccgccaag
     1021 tacgcgggga tgaacattat cgccaccacc attatcggta tggctgcctc cggtgcgctc
     1081 gccggtatgg caggcgccaa tgaagttttg gcggtgaacc gcagcatggc tatcggactc
     1141 tcggctggtt atggttttga cagcatcgcc ctggcgctgc ttggcaacag tcatccggtt
     1201 ggtgtcattt tcgctgccct tttatttggc gtgcttaaga atggcgccac caagatgatg
     1261 gtggtctcca ccattcccat tgatattgtc accatcctgc aggcagtcat ccttattttc
     1321 gtcgccgctc ctgcgatcat tcggtcagtc tatcggctta aacaaccaaa agtggaggcc
     1381 gctgccatca tgctcagcgg ttggggagga gttaaataat ggcagccaca tcaacttcca
     1441 tccaacgtgt ccgcatttct gatatctccc ctgcccgtaa gatcagcacc ggtgttgtcg
     1501 aagttttagt tgccttgttt atctttgccg tctttgccag aaccgtctct gcgggcagca
     1561 tcaccacttt tgtcatgacc ccgggcggga tcaccactgg tcagatgggc aactgggtaa
     1621 ttcccagcca gcttacgctg tacctgctcg ctgcgctcag cctggtgatc ggactttttc
     1681 agttgatacg cgggttcggg aaaatcacca atctcatggt tggattggtg ttcttgttct
     1741 tcattttcag tttcctcacc tggcaagcgg ctgataaatc cttaaacctc gctggtatgc
     1801 tttccagcgc agtacagctg gctgtgccta tcaccatggg ttccttctcc ggtatcttat
     1861 gcgagcgctc aggcgtggtc aacatcgcca ttgaaggcat gatgctaatg tctgccatgg
     1921 tcggagcctt gatgggcagc ctcacgcata gcgcgtggct cggtttattc ttcggcgttc
     1981 tttctgcggt tttactggct gccctgcacg cggtcctgtc gatcaaatat aagattgacc
     2041 agatcatttc tggcacggtg attaatatgt tctcagcggg tatgaccgct tttctttctc
     2101 aaaagttcct gcaaaccagg caagagctga acaaccccgc catgttcacc cgaattgaaa
     2161 tccccggtct ggcggaaatc cccttaattg gtccgattct gtttaacacg aacttttttg
     2221 tctacctgat gttcgcactg cttattattc tacaggtggc gctcttctcc actcgctggg
     2281 ggttgcgcgt gcgcagtgtg ggcgagcatc ccaaggcagc cgatacactg ggcatcaacg
     2341 t
//
LOCUS       DAOTLW010000102         2318 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_37373_length_2318_cov_9.298719, whole genome shotgun sequence.
ACCESSION   DAOTLW010000102 DAOTLW010000000
VERSION     DAOTLW010000102.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2318)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2318
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37373_length_2318_cov_9.298719"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="P5516_08840"
     CDS             <1..527
                     /locus_tag="P5516_08840"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT92572.1"
                     /translation="RVNPQYRRVKDPYGGDDVIVVPALNPDVAIVHVQRTDSEGNAHI
                     WGILGEQREAAFAAKHVILTAEEIVDESVIRSDPNRTLIPAMIVDAVCHVPYCAHPSY
                     TQGYYDRDNEFYLAWEEISKDEASVKAYLDEWVFGVRDRAEYWQKLGAETHRRLHVGE
                     RLSTPVNYGSFANE"
     gene            520..1284
                     /locus_tag="P5516_08845"
     CDS             520..1284
                     /locus_tag="P5516_08845"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HRT92573.1"
                     /translation="MSEAAYNATELMIINAARLLQDGDQVFVGVGIPNLACNLARRTH
                     APHLQMIYEAGVIGAQPERLPLSIGDPTLVSGALAVCSMFEIFSFYLQRGNIDVGFLG
                     GAQIDQFGNINATVIGDYFHPKVRLPGSGGSMEIAAWANRCYIITPHQKRRFPARVDF
                     HTSIGFLEGGDARAKTGVRGAGPQAVVTNLCILKPNEQGELILAALHPGCTFEEVQAN
                     TGWQLKQAPHVEVTPAPTQEELRILREELDPQRIYI"
     gene            1285..2310
                     /locus_tag="P5516_08850"
     CDS             1285..2310
                     /locus_tag="P5516_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014841.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-2-hydroxyacid dehydrogenase"
                     /protein_id="HRT92574.1"
                     /translation="MSENKPLEVLSTIDLSEAQQEYIKNQVKNIHLTVVSAKDAANLP
                     KEVWEKAEVLLTSGRNLPTREQAPALKWIQVSFAGVEHALEYPISSAPGVLLTSASGV
                     RVSQLGEYALMALLALSHKLPKTIQAQREKKWLPNSSDVLMPQEIRESTVGIVGYGSI
                     GREVARLLYSFGATVLAAKHNVMQPEDNGYNAQGLGDPKGNYFRRLYPIEGLPGMLKE
                     CDFVVLTLPLTKETYHLFDDKMFEAMKPGAGFVNIARGGLVDEAALVAALKSRLSGAV
                     LDVFEQEPLPPESPLWSLPNVIITPHISGISPKLMDAVVELFVKNLQRYQAGEPLYNI
                     VDTSKGY"
BASE COUNT          547 a          626 c          643 g          502 t
ORIGIN      
        1 agcgcgtgaa cccgcaatac cggcgcgtga aagaccctta tggtggggac gatgtgattg
       61 tagtaccagc gctcaaccca gatgtggcga ttgtgcacgt ccagcgtact gatagcgagg
      121 gcaatgcgca tatttggggc atcttaggcg agcagcgcga agccgctttt gcggctaaac
      181 acgtaatcct caccgccgag gagattgtgg atgagagcgt gatccgctct gaccccaacc
      241 gcacgctcat ccctgccatg attgtagatg ccgtctgcca tgtgccctat tgtgcacatc
      301 cttcttacac ccaagggtat tatgaccgcg ataatgagtt ttatttagct tgggaagaaa
      361 tcagcaagga tgaagcgagc gtcaaggcgt acctggacga atgggtgttt ggtgtgcgcg
      421 accgggcaga gtattggcag aagcttggcg cagaaacaca ccgccgcctg catgttgggg
      481 agcgcctaag caccccggtt aattatggga gctttgccaa tgagtgaagc agcatataac
      541 gccacagaat tgatgattat caacgccgcc cgcctcctcc aggatggcga ccaggtcttt
      601 gtgggtgtgg gcatccccaa cttagcctgc aatcttgccc gacgcacgca tgccccgcac
      661 ctgcagatga tttatgaagc cggcgtgatt ggcgcccaac ccgaacgcct gccgctttcg
      721 attggcgacc ccaccctcgt gagcggggcg ttagcggtct gctcgatgtt cgaaatcttt
      781 tcattctatc tgcagcgcgg caatattgac gtcggcttct taggcggcgc gcagatcgat
      841 caatttggca atattaatgc tactgtgatt ggtgattact tccaccccaa ggtgcgcctg
      901 ccaggttccg gcgggtcgat ggagatcgca gcctgggcaa accgttgtta catcatcacc
      961 ccgcaccaaa agcggcgctt cccagctcgg gttgactttc atacttcgat tggattttta
     1021 gaaggcggtg atgcccgcgc taaaacgggc gtgcgcggcg ctggtccaca agccgtggtc
     1081 accaacctgt gcattctcaa gccgaatgag caaggagaac tgatcttagc tgcactgcac
     1141 ccaggttgca cctttgaaga ggtacaagct aacaccggct ggcagctcaa acaagcccct
     1201 cacgtggaag tgaccccagc cccgactcag gaagaactaa ggattttgag agaggaatta
     1261 gaccctcaga ggatttacat atagatgagt gagaataaac ctttggaagt gctgagcacg
     1321 atcgatctga gcgaagctca gcaggaatac atcaaaaatc aagttaaaaa tatccatttg
     1381 accgtagtga gcgctaaaga tgctgcaaac ttacccaagg aggtctggga aaaagcagag
     1441 gtactgctca ccagcgggcg caatttaccc actcgggaac aagcaccggc gctcaaatgg
     1501 attcaagttt cctttgccgg tgtggagcac gcgctggaat atcccattag ctccgcgcca
     1561 ggtgtgctgc tcacttctgc cagtggggtg agggtgagcc aattaggcga atatgccttg
     1621 atggcgctac tggcgctttc ccataaactg cccaaaacta tccaagccca gcgtgagaaa
     1681 aagtggctgc ccaactccag cgatgtgctg atgcctcagg aaattcgcga aagtactgta
     1741 ggcatcgttg gttatggctc gatcggccgc gaagtagcgc gcctgctcta ctcctttggc
     1801 gccaccgtgc tggctgccaa gcacaacgtg atgcagccgg aagataacgg ctataacgca
     1861 caaggcttgg gcgacccgaa aggtaactac tttagacggc tctaccccat cgaggggctt
     1921 ccgggcatgc tgaaagaatg cgatttcgtg gtgctcacct taccgctcac caaggagact
     1981 tatcaccttt tcgatgacaa gatgttcgag gcgatgaagc caggggctgg ttttgtcaat
     2041 attgcccgcg gcgggctggt ggatgaggct gctcttgtag ccgccctaaa aagccggctc
     2101 agcggggcgg ttttggacgt cttcgagcag gagccgctac cgcccgaaag ccccttatgg
     2161 agtctgccca acgtgattat cacgccgcat atttctggca tcagccccaa gctgatggac
     2221 gctgtcgtcg agcttttcgt aaaaaacttg cagcgctacc aagccggcga gccgctttac
     2281 aacatcgttg atacgagcaa aggctactaa ggcactca
//
LOCUS       DAOTLW010000103         2298 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_37792_length_2298_cov_9.043691, whole genome shotgun sequence.
ACCESSION   DAOTLW010000103 DAOTLW010000000
VERSION     DAOTLW010000103.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2298)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2298
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_37792_length_2298_cov_9.043691"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(1..1233)
                     /locus_tag="P5516_08855"
     CDS             complement(1..1233)
                     /locus_tag="P5516_08855"
                     /inference="COORDINATES: protein motif:HMM:NF019561.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HRT92575.1"
                     /translation="MLARLRAFGKLIPLIIVSMVVAVAVWILAVTSSDPSETRLYAKP
                     VPIEVVGQSTNLVITNHLPESVTVNLRAPVSIWEKINSGKAGVRAFIDLSGVPEGTRT
                     TQVQVQVGIKPVEVISYSPQMVDLVLEKLSNKRFDIAVVSRGALPVGYQTEDPRLSET
                     SAVVSGAASLVAQVAEVRAVVDLSQVRSDINQEITLQPVDANGLLVREVSVTPDKITL
                     LQGVSQRGGYRNVVVKVVTEGLIKDGYRLKSVTVNPPTVTVYSSDTATVDALPGYVDT
                     RVINLVDRQEGFSVDVELSLPAGLQVIGSSMVEVKVEIEPVVSSLSLSEIQLEPTGLA
                     SNLSAVIVPNKANIILSGPVPLLNSLFVNDLRILIDLTGRLPGVYSIDVKPTLNIPGL
                     EIESISPATFEVTIRTKK"
     gene            complement(1233..2114)
                     /gene="cdaA"
                     /locus_tag="P5516_08860"
     CDS             complement(1233..2114)
                     /gene="cdaA"
                     /locus_tag="P5516_08860"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HRT92576.1"
                     /translation="MIDLWSEIVYIFQRMNWLSVLDLFLVAAVVFAVLLLLRDTQIMV
                     LLRGVVVIILLLVLLTTFINLPAFSWLVTTIVPALLFAIPVIFAPEIRRGLEKLGRAG
                     SSQLLVRQTESQIQLMEKTIRSIVTAASRLSAREHGALIVMQRGVSLEEYARTGVRLN
                     ASVTPELLLQIFYPNTPLHDGAVIIADNKIVAGACVMPLSSSGILTRSPERQMGLRHR
                     AALGTSEATDAITVVVSEETGSISIAHNGRMIRRLDSERLENILLAFYQPVEHPVFKF
                     KPFDWFKKIVSVQKRED"
BASE COUNT          559 a          677 c          584 g          478 t
ORIGIN      
        1 ttactttttt gtacgaatgg tcacttcgaa agtggcggga gaaatgctct caatctcaag
       61 cccggggata ttcaacgtgg gtttcacatc gattgagtaa actcccggca ggcgaccggt
      121 cagatcgatc aggatgcgta aatcgttgac aaacaagctg ttcagcaacg gcaccggacc
      181 ggagagaata atgttggctt tgttcggcac gatgaccgcg ctcaggttag aagccagccc
      241 ggttggttcc agttgaattt ctgataacga caaactgctc accaccggtt caatttccac
      301 cttcacttct accattgagc ttccaatgac ttgaagcccc gccggcaggc tcagttctac
      361 atccacgctg aagccctcct ggcggtctac gaggttgatg acgcgggtat ccacataacc
      421 gggcagcgcg tccactgtgg cggtatcaga agagtaaacc gttacagtag gcgggttgac
      481 agtcacgctc ttcaaccggt agccatcttt gatcaacccc tctgtaacca ccttaaccac
      541 cacgttacga tagcctcctc gctgcgatac cccctgcagc aaggtgattt tatctggggt
      601 cacactgacc tcgcgcacca atagcccatt ggcatccacc ggttgcaagg tgatttcctg
      661 gttgatatct gaacgcacct gtgacaggtc aaccaccgcc cgcacctccg ccacctgcgc
      721 gaccaaagac gcagccccgc ttaccaccgc tgaggtttct gacaaccggg ggtcctcagt
      781 ctggtacccc accggcagcg cgccgcggct gaccaccgcg atatcaaagc gcttgttgct
      841 cagtttctcg agtaccaggt caaccatctg cggagagtat gaaattactt caaccggctt
      901 gatacccacc tgtacctgaa cctgcgtggt gcgcgttcct tccggcaccc ctgaaagatc
      961 gatgaaagcg cgcacgcccg cttttcctga attgattttt tcccagatag aaaccggcgc
     1021 gcgcaggttc actgtgacgg attcgggtaa atggttggtg atgaccaggt tcgttgattg
     1081 accaaccact tcaatcggca ccggcttcgc atacaaacgg gtctctgaag ggtcggaaga
     1141 agtgaccgcg agaatccata ccgcaaccgc caccaccatc gatacgatga tgagcgggat
     1201 caatttgccg aacgcgcgta atctggccag catcaatcct cccgcttctg cacactgact
     1261 atcttcttga accagtcaaa gggtttaaac ttaaacaccg ggtgttcaac cggttgatag
     1321 aaagccagca gaatattctc gagccgttcg ctgtccaaac ggcggatcat ccgcccgtta
     1381 tgcgcgatgg aaatcgaacc cgtctcttca gacacgacca cggtgatcgc gtctgtggct
     1441 tctgaagtgc ccaaggcagc cctgtggcgc agccccatct gccgctcagg cgagcgcgtg
     1501 aggataccgc tcgatgaaag aggcataacg caggcaccag cgacaatctt gttatccgcg
     1561 atgatgaccg cgccatcatg cagcggcgta ttaggataaa aaatttgcag caacagctca
     1621 ggcgtcactg aggcattcag gcgcacacca gtgcgcgcat attcttccag gctgaccccg
     1681 cgctgcatca caatcagcgc tccgtgttcc ctggcagaga gccgcgaggc tgccgtgacg
     1741 atcgaacgga tggtcttttc catcagttga atttgcgatt cagtttgccg taccaacaac
     1801 tggctcgaac cggcccgccc aagtttctca agcccacgcc tgatctccgg cgcgaagata
     1861 accggaatgg caaagagtaa agccggcaca atagtggtga ccagccagga aaaggcgggg
     1921 aggttaataa aggtggtcag cagcaccagc agcaagatga ttaccaccac cccgcgcaac
     1981 aacaccatga tctgcgtgtc acgcagtaat aacaacacgg cgaaaacgac tgcggcaacc
     2041 aggaacaggt ccagaacgct gagccagttc atgcgctgga agatgtagac gatttcgctc
     2101 cacagatcga tcatatcaac ctaattttac ctgcgcgcag gtaaaaagca attaaatcgg
     2161 gcgcagcacg cggtcctggc gcaatggctt gacaaacaag atcgaatcca gctgcatgga
     2221 ttcaagggcg gcttccttcc aggcgagcac gttcaggtca tcgggcttct tttccgcgta
     2281 acccaggctg atccacag
//
LOCUS       DAOTLW010000104         2279 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_38194_length_2279_cov_16.677608, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000104 DAOTLW010000000
VERSION     DAOTLW010000104.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2279)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2279
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38194_length_2279_cov_16.677608"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..958)
                     /gene="ftsZ"
                     /locus_tag="P5516_08865"
     CDS             complement(<1..958)
                     /gene="ftsZ"
                     /locus_tag="P5516_08865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HRT92577.1"
                     /translation="MDAKQVELSARIKVVGVGGGGCNAVDRMIAEGLSGIEFIAINTD
                     AQVLLKSSAPTRVRIGDKTTRGLGAGGDPNIGKISAEESAEELYDVLKGSDMVFVTAG
                     LGGGTGTGAAPVVAQIAKEIGALTIGVVTRPFAFEGQRRAIAAEEGALRLKEHADTLI
                     VIPNDRLMQMVTKSVSMCDAFRLADDVLRQGIQGISELITVPGLINLDFADVRTIMSE
                     GGAALMAVGEASGEDRAKVAAEKAISSQLLDVTINGAHGILFNVTGSSNLSLFEVNQA
                     ASIIKETASPDVNLIFGAVIDESMGDKVRITVIATGFDHNHSP"
     gene            complement(988..2226)
                     /gene="ftsA"
                     /locus_tag="P5516_08870"
     CDS             complement(988..2226)
                     /gene="ftsA"
                     /locus_tag="P5516_08870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HRT92578.1"
                     /translation="MEEPIVVGLDIGTSKICTLVARIENGANLRILGVGVEPSQGIRK
                     GVVIDINSASQSIARSIEKAERTSGYEINSALVSLAGSHVSSMNSRGVVGISGRVIDQ
                     EDVLRALDSAQAVAIPHDREIIHVIQRGFLVDGQDGIRQPVGMHGFRLEVEAHIITAS
                     ASTTENLRKCVSMAGIEVSQFVLSPLASAEAVLSETERTMGAAVVDLGGGTTDLAIYL
                     NGDVWYTNVINIGSNYITTDIAQGLRLPVDVAEEVKLHHGYAVLDEISEEESFNVVPF
                     SAEESTKVLRRDLVNIIEARAEEILSLVHQEIKRSGVSGLLPAGLILTGGGSQLPGMR
                     KLATQVTGLPARIAKPEKMVGLTDQISSPAFSTSVGLLQWAILMSETYQIGSERPGKG
                     KRSEGERTETFASWFKKLLP"
BASE COUNT          560 a          621 c          545 g          553 t
ORIGIN      
        1 tgggagagtg gttgtgatca aagccggtag caatgacggt gatgcgcacc ttatcgccca
       61 tagattcatc gatgacagcc ccaaagatta aattgacatc cggagacgct gtttctttga
      121 tgatcgaagc tgcctgatta acctcaaata gcgagaggtt agaactgcca gtgacgttga
      181 agaggatgcc atgagcacca ttaatggtca catcgagcag ctggctggaa atcgctttct
      241 cagcggcaac cttcgcgcgg tcttctcctg aagcttcgcc cacagccatc aaagccgccc
      301 cgccttcgga cataatcgtg cgcacatcgg caaagtcgag gttaatcaac cctggaacgg
      361 tgatcagctc agaaataccc tgaatccctt gacgcaagac atcatccgca agcctgaaag
      421 cgtcgcacat gcttacgctc ttggtcacca tctgcatcag gcggtcattg gggatcacta
      481 ttaaggtatc agcatgttcc tttaagcgca aagcaccttc ttctgcagct attgcacgcc
      541 gctgaccttc gaaggcaaac gggcgagtga ccacaccaat ggtgagtgcg ccgatttctt
      601 ttgcgatctg tgccacgaca ggcgctgcgc ccgtgcccgt gcctccaccc aaacctgcgg
      661 taacaaacac catatcgctg cctttgagca catcatagag ctcttcagcc gattcttccg
      721 cggagatttt gccgatattg gggtcaccac cagcaccaag tccgcgggtg gttttatcgc
      781 cgatgcggac acgggtgggt gcgctgctct tgagcaacac ctgagcgtca gtattaatgg
      841 caatgaattc tattcctgat aacccttcag cgatcatgcg atcgacagca ttacagccgc
      901 caccgccaac gcctactact tttatgcgtg cacttaattc aacctgtttt gcatccattt
      961 ttctcctctc atggcttgaa taattgatta tggtaataac tttttgaacc aggaagcaaa
     1021 cgtctccgtg cgctcgcctt ctgatcgttt cccttttcca ggtcgttctg atccgatctg
     1081 gtaagtttca ctcattaaaa ttgcccattg tagtaagcct acgcttgtgg aaaacgccgg
     1141 cgaactaatc tggtctgtca agccgaccat tttctcaggt ttggcgatgc gggctggtaa
     1201 accggttact tgcgtcgcta acttacgcat gccaggcagc tggcttccac ctccggtcag
     1261 gatcagccca gcgggaagca aacctgaaac gccagaacgc ttgatttcct gatggaccaa
     1321 ggacaagatt tcttcggcgc gtgcttcgat aatattgacc agatcacgcc taagaacctt
     1381 ggtgctttct tcggcactaa aaggcaccac attaaagctc tcttcctcag aaatttcatc
     1441 caagacggca tagccatggt gtaatttcac ttcctcagcg acgtcaaccg gcaagcgtaa
     1501 gccttgagca atatcggtgg taatgtaatt gctgccgata ttgatcacat tggtgtacca
     1561 gacgtcacca ttgaggtata tggctaaatc ggtagtgccg ccgcccaggt cgaccacagc
     1621 tgcacccatg gtgcgttcgg tctcggaaag caccgcttcg gcagatgcca aggggctgag
     1681 cacaaattgt gagacctcaa tccctgccat ggaaacgcac ttacgtaagt tttcggtggt
     1741 ggaagcggaa gcggtgatga tgtgcgcttc tacctccaag cggaaaccat gcatcccaac
     1801 gggctgacga atgccatctt gtccatcgac taagaaaccg cgctgaatca catggataat
     1861 ttctcggtcg tgggggatag ccacggcttg ggcggaatct aaggcacgca aaacgtcttc
     1921 ctggtcgatg acgcggccgg aaatacccac aacgccgcgg ctgttcatcg aggaaacatg
     1981 cgagccagct aagctgacca aggcgctatt gatttcatag cctgaggtgc gctctgcttt
     2041 ctcaatggaa cgagctatcg attgggaggc agaattgata tcgatcacaa cacctttgcg
     2101 gatgccttgt gaaggctcaa ccccaacccc caaaatacgc agattggcgc cgttttcaat
     2161 ccgagctacc aaagtgcaaa ttttactggt tccaatatct aatccgacaa caattggctc
     2221 ttccatagtt actactccaa tcgataataa ggtgcgtgta tatattcaac gctgattaa
//
LOCUS       DAOTLW010000105         2270 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_38383_length_2270_cov_11.072235, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000105 DAOTLW010000000
VERSION     DAOTLW010000105.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2270)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2270
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38383_length_2270_cov_11.072235"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..865
                     /locus_tag="P5516_08875"
     CDS             <1..865
                     /locus_tag="P5516_08875"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024760.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PrsW family glutamic-type intramembrane
                     protease"
                     /protein_id="HRT92579.1"
                     /translation="FIPLWWLLELGRLRLARCSTQRQWGVTAFSFYITLPVVVIVEII
                     LFVLVVLLAAVWLVQQPEFSPLLEQFERAFASGSFNPQNFTFDWLPLLEKPGVIVVIV
                     LSISLVVPVMEELLKPLGIWALVKRGLTPLDGFVAGMLCGASFALFESLFSLSSVSGE
                     EWLFIVTGRAGTGLLHLTLTGFNGWALASSWKDGKTARVALTYILTVFVHGAWNLFAM
                     VMGLSMTGEELHLSVSPVLSASAPWVLGSLALGMLVMLIVMNWRLRRAPAASPSSAAP
                     PPLPAATRGLE"
     gene            919..1611
                     /locus_tag="P5516_08880"
     CDS             919..1611
                     /locus_tag="P5516_08880"
                     /inference="COORDINATES: protein motif:HMM:NF015251.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HRT92580.1"
                     /translation="MKSHRLRSIIGFLLKQLTQTEFIGVENIPPEGGVLIACNHMSRL
                     DIPVLFITPNRPEITALVTTKYLKYPLLRWFIVTAEGIWLDRDTADFSAFRKAVEALK
                     QGKALGIAPEGTRSQTAKLIEGKPGTALLALRTGVPIVPVAIVGTEDGMSKLLRLKRP
                     RITAEYGKPIIPPKLDRSNREGQLQNLTDEVMCRIAAMMPQKYHGFYADHPRLKELLA
                     EQKKNAAGRLGG"
     gene            1617..>2270
                     /locus_tag="P5516_08885"
     CDS             1617..>2270
                     /locus_tag="P5516_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92581.1"
                     /translation="MVSQEQTGNTHNQPCVPTPARRQRPRSLLTSLLLGACLFLGLLF
                     VFEGLFRWTDLDRIFGWRSLGTYHGQFEIKWFELRDYVRSHGGVDVLLLGNSMVNTGI
                     DPDVLATEYEVLTGERLRVFNFGVEGLTVAPNSHLARVLAARYHPGSILFFTEMRDYA
                     AGNGVEVERQLLADEWMKARLGGKETGRAWLKENSRVVQHLLFFRNWSRSDFLDSSLM
                     "
BASE COUNT          467 a          607 c          644 g          552 t
ORIGIN      
        1 ctttattcca ttgtggtggt tgttggagct ggggaggcta cggctggcga gatgcagcac
       61 ccagcggcag tggggggtta ccgctttcag cttttacatc accctgccag ttgtggtcat
      121 tgtggaaatt attctcttcg ttctcgttgt gctgctggcg gcggtgtggt tggtgcagca
      181 acctgaattc tcgcccctgc tggaacaatt cgagcgggcg tttgccagtg gttcatttaa
      241 cccgcagaac ttcacgtttg actggctgcc gctactggaa aaacccggtg tcatcgtggt
      301 catcgtgttg agtatttcgc tggttgtgcc agttatggaa gaactgctca aaccactggg
      361 aatatgggca ttggttaaaa gagggctcac tcccttagac ggcttcgttg ctggaatgct
      421 ctgcggggct tcgttcgccc tgtttgagag cctgttttct ctcagcagcg tgagcgggga
      481 agagtggctt ttcattgtta cgggtcgtgc aggaacgggt ttactgcatc tcaccctcac
      541 cggttttaac ggctgggcat tggcttcttc atggaaagac ggaaaaaccg cgcgtgttgc
      601 ccttacctac atcctcactg ttttcgtcca cggcgcatgg aatctttttg ccatggttat
      661 gggtttaagt atgacaggcg aggaacttca tttgagcgtc agccccgtac tctctgcttc
      721 tgctccctgg gtgctcggct ctctggcgct ggggatgctg gtgatgttga tagtgatgaa
      781 ctggcgttta cgccgcgcgc ctgcggcttc tccatcatcc gccgcgcccc caccgctccc
      841 ggctgccacc aggggattgg agtaaaatga acctgccgtc agaataccgc tatcaaccac
      901 aggaaggtgc aaaataagat gaagtcacat cgattgcgca gcattattgg tttcttgctg
      961 aaacaactga cccaaacaga attcatcggc gtagagaaca taccccctga aggtggtgtg
     1021 ctgatcgcct gcaaccacat gagtcggctg gatatccctg tcctttttat cacgcctaac
     1081 cggccagaga taaccgccct ggtgaccacc aagtatctaa aatacccttt attgcgctgg
     1141 tttatcgtca ccgctgaggg tatctggctg gaccgtgata cggctgattt ttcagccttc
     1201 cgcaaggccg tggaggcctt gaagcaagga aaagcgttgg ggatcgcgcc tgaaggcacc
     1261 cgcagccaga cggcgaaact gattgaaggc aaacccggta ccgcgctgct ggctctgcgc
     1321 accggggttc ccatcgtccc ggtggcgatt gtaggtaccg aagacggtat gagcaagtta
     1381 ttgcgcttga aacgcccgcg catcactgct gaatacggta aacccatcat cccgccaaag
     1441 ctggatcgca gcaaccgcga aggacagctg caaaacctta ctgacgaagt gatgtgccgc
     1501 atcgctgcca tgatgcccca aaagtaccat ggtttttacg cggatcaccc gcgcttgaaa
     1561 gagctgctcg cagaacaaaa gaaaaacgcc gccggacgcc tgggcgggta gaagaaatgg
     1621 ttagccaaga acaaactggg aacactcata accaaccgtg tgtaccgacg cctgcgcgca
     1681 ggcaaagacc acgctcgctg ctcacctcgc tcctgttggg cgcctgtctc tttcttggtc
     1741 tgcttttcgt gtttgagggt ttatttcgct ggactgatct tgaccgtatc tttggctggc
     1801 gctcactggg cacctatcac ggtcaatttg agatcaagtg gtttgagctg agagattacg
     1861 tccgctctca cggcggtgtg gatgtgctgc tgctgggcaa ctcgatggtg aacacgggta
     1921 ttgaccccga tgtgttagca acagaatatg aagtgctcac cggtgaacgc ctgcgcgtgt
     1981 tcaacttcgg cgtagagggg ctcacagtcg cgccaaattc gcatctcgcg cgcgtgttgg
     2041 cggcgcgtta tcacccgggc agcatccttt ttttcaccga aatgcgcgat tacgccgccg
     2101 gtaatggagt ggaggtggag cgccagttgc tggctgacga atggatgaag gcccgcctgg
     2161 gcggaaaaga aaccggtcgc gcctggctta aagaaaattc ccgcgtagtg cagcacctgc
     2221 tcttcttccg aaactggagc cggagtgatt tcctcgactc ctccctgatg
//
LOCUS       DAOTLW010000106         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_38872_length_2249_cov_10.884230, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000106 DAOTLW010000000
VERSION     DAOTLW010000106.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38872_length_2249_cov_10.884230"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(6..1565)
                     /locus_tag="P5516_08890"
     CDS             complement(6..1565)
                     /locus_tag="P5516_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92582.1"
                     /translation="MKKLSITTRDLLLTLIAALAISIGMAVFQRAPFFEAWFGSWLLL
                     WVSLFLLIKTWRFFQGGRWLAVLLLVCFFLRVFLGLFIYIALPQIGNDNEVENAGYVY
                     SDAYHRDTQALVWAKSETPLVSIFSSKNSRDQYGGLQFISASLYRLFSVDEPRPLFTT
                     ILAAMTMSVGVPFLYDALKRRWGAGVARVAAWFYTLYPDSVLLGSSQMREPFLIGLFC
                     IAFWMVVTRREKTTRKLLFFLLLTGVSLAISLPLGGLLAGLLIMWGLIEWITEQKTPR
                     SKTTGIILLILASIAALTAGYIWVKPTVYYDAYLTRTASGLIAALFKLIDEKWHIPLT
                     TFYGLAQPFLPAALVYPSLPFWRVTAIIRGLGWWFMLPFLLYGFFAVWKAKPRSERWL
                     MAFIALALTAWTVVTSLRAGGDLWDNPRYRALLIPWFALLLGWCWQRIRQGHLAWFLR
                     WTGVVLVFFSVILLWYLLRYHIIKGYLGFFDMALIITLASAGILLSGLLWDGYKWFTA
                     RKKKQQPDGSG"
     gene            complement(2133..2205)
                     /locus_tag="P5516_08895"
     tRNA            complement(2133..2205)
                     /locus_tag="P5516_08895"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2170..2172),aa:Ile,seq:cat)
BASE COUNT          656 a          596 c          546 g          451 t
ORIGIN      
        1 gaaattcacc cagaaccatc aggttgctgc tttttcttac gcgctgtgaa ccatttatat
       61 ccatcccaga gcaacccgct gagcaagatc ccagcagatg ccagggtgat tatcagtgcc
      121 atatcaaaga aacctaaata tcctttgatt atatggtaac ggagcaaata ccaaagcaaa
      181 atgaccgaaa agaacaccag caccactccc gtccagcgca ggaaccaggc cagatgaccc
      241 tgcctgatgc gctgccagca ccaaccaagc agcagggcaa accatgggat gagcagcgcg
      301 cgatagcgcg ggttgtccca gaggtctcct ccagcgcgaa gagaagtgac aaccgtccac
      361 gcggttaacg ccagcgcaat aaacgccatc aaccagcgtt ctgaacgcgg tttggccttc
      421 caaacggcaa agaacccgta aagcaagaag gggagcatga accaccaccc taacccccgg
      481 atgatcgcgg ttacacgcca gaacggcaat gaaggataaa cgagcgcggc tggcaagaaa
      541 ggctgggcca aaccataaaa agtggtcagt ggaatatgcc acttttcatc tatcagttta
      601 aacaacgccg caattaagcc tgaagcagta cgggttaagt aagcatcgta atagaccgtg
      661 ggtttgaccc atatataccc ggctgttaag gccgcaatac tcgcgaggat aagcagtatg
      721 attcctgttg tcttgctgcg cggcgttttc tgttcagtta tccattcgat caacccccac
      781 attatcagca atcccgccag gagccctccc aatggaagcg agatcgccag cgaaacaccc
      841 gtcagcagaa ggaagaaaag cagctttcgc gtggttttct cgcgcctggt gacaaccatc
      901 caaaaggcaa tgcagaaaag cccaatcaag aaaggctccc gcatctgcga gctgcccaac
      961 aacacactat cagggtagag tgtgtaaaac caggcggcaa cccgggcaac accggctccc
     1021 caacggcgtt tcagcgcatc atacaggaaa ggcaccccca cgctcatagt catggcggcc
     1081 agaatcgtag tgaacagcgg gcgcggttca tccacgctga ataagcgata gagcgaggct
     1141 gaaataaact gcagtcctcc gtactggtct ctgctgttct tgctggaaaa tatagagacc
     1201 aatggcgttt cacttttcgc ccatacaagt gcctgggtgt cacggtgata cgcgtcactg
     1261 taaacataac cagcgttctc cacctcgttg tcgttaccaa tctgcggtaa agcaatatag
     1321 atgaaaagac caaggaatac gcgtaagaaa aaacatacca gcagtaagac agccagccat
     1381 ctgccaccct ggaaaaaacg ccaggtcttg atcaacaaaa acaggctgac ccatagcagc
     1441 agccagctgc caaaccaggc ttcgaagaat ggcgcacgtt ggaacactgc catacctata
     1501 ctgatggcca gcgcagcaat caatgtgaga aggaggtcgc gagtagtaat gctgagcttt
     1561 ttcatcggaa aaacaatcat tcagggtagc tgttattata acttggaaaa acacccgggg
     1621 aaaagcaaag aaaatgacca tcagcaaact gctgttatgc caattcagta caaaatgaac
     1681 caaccggttt ttgcccaatc acaacacctt cttttccggc gaaaaatgaa cgcggattct
     1741 tgcgcaaata gatgggtttg agaaaggagt cgcgtatgac cacggcttag gagttggcgt
     1801 caattttctt tacagagatt tttcgctttt tcgctggggg aaggtcacat gaagcgcggg
     1861 tgacaccttc ctgaaggctc tcatccatat tctaagaatg gaagcccgcc ttgcgggtga
     1921 accactactg aagtgcagca agcatgcgcc ggccaggcct tgctgcccgc agccctttct
     1981 tcctgggttc agaaaaccag tgaattgcgt cagcgcatgc acaatctaat aaagaccatt
     2041 tttcgatttg aagagttgaa aggtgattta cgggttccgt gccatggtgt ttggtaatga
     2101 aacccctggt gaaccaaaaa tgacctgggg agtgggcccg gcagggatcg aacctgcgac
     2161 aaagcggtta tgagccgcct gctctaacca ctgagctacg ggccccaata cccgcatcga
     2221 ttataacatt aagaaattga accgtgctc
//
LOCUS       DAOTLW010000107         2249 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_38873_length_2249_cov_10.371468, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000107 DAOTLW010000000
VERSION     DAOTLW010000107.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2249)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2249
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_38873_length_2249_cov_10.371468"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1241
                     /locus_tag="P5516_08900"
     CDS             <1..1241
                     /locus_tag="P5516_08900"
                     /inference="COORDINATES: protein motif:HMM:NF004720.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HRT92583.1"
                     /translation="LVKPGKLIVPDRLHGYNLIRTNNEVKMRQVAVIGIGQTKIDEHW
                     DKSLRDLAGDAALAALKDSGLERVEAVYVGNMMSGSANHQQHLGAYLADWIGMRGAEA
                     IRTEAACSSGAASFRSAVMAVASGLVDSALAVGVEKMTDSPLNEITAALATAADAEWE
                     AGNGLSFVSLNALLMKRYMYEYGWEKDHFAPFAVNAHANAMHNPYARFHEPVTVEGYL
                     KAPMITDPINLMDASPTGDGAAAALVVPIEKFHQNNGRKIIRVAASSAATDTMAVHHR
                     KDALFLKAAYNAATKAYAQSSLTPQDIDLFELHDAFSIMATLSLEANGFAEKGKGPQL
                     ALDGEILTNGRIPIATRGGLKARGHPVGATGMYQIVEVVEQLRGEAAGTQVENARVGM
                     TQNIGGSGSNIISHIFAVTD"
     gene            1301..2164
                     /locus_tag="P5516_08905"
     CDS             1301..2164
                     /locus_tag="P5516_08905"
                     /inference="COORDINATES: protein motif:HMM:NF017984.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HRT92584.1"
                     /translation="MAIVSASRLTDYRALTFRFSPGFSLSTPEQAVEYVKERGFIFFW
                     PVKGFTFPSLWGAVAGDRPVTDEHDDPGHITWHWKDSLLDQRVWYYARVLRRKNTLIS
                     LDVLKYFYALSPNYGEPELDLIDQYNQGELPLEAKLIFEALNKNGPLDTLSLRKEARL
                     AGVNSQSPFNRALDILQAQLRVLPVAVSEAGAWKYAFVYDLTHRYYPQLSESARFITE
                     NQAMDYLLRLFFKSLGVASLRQLTHTFRWDAGLTIKAIERMIQEGYLMKDVKVEGSPE
                     NHYCLVELFEA"
BASE COUNT          547 a          562 c          590 g          550 t
ORIGIN      
        1 ttcttgtaaa accaggtaaa ctgatagttc ctgataggtt gcatgggtat aatctcatta
       61 gaacgaacaa cgaggtgaag atgagacagg ttgctgttat tggaattggc caaacaaaaa
      121 ttgatgagca ttgggataaa tcgcttcgtg atctggcggg cgatgccgcc ctggcggcgc
      181 tgaaggattc cggtctggag cgagtggaag ctgtttacgt cggcaacatg atgtctggct
      241 cggccaacca tcagcaacac ctgggagcgt atttggcaga ttggattggt atgcgcgggg
      301 cggaagcgat tcgtaccgaa gccgcgtgca gttcaggagc ggccagtttt cgctccgcgg
      361 taatggctgt tgcctctggt ctggtggact ctgccctcgc tgtcggggtt gaaaagatga
      421 cggattctcc cctgaatgag attaccgccg cccttgcgac agctgcggac gcggaatggg
      481 aagcgggtaa tgggctttca tttgtgtctt tgaacgccct tttgatgaaa cgctatatgt
      541 acgaatatgg ctgggagaaa gaccacttcg cgcctttcgc ggtcaacgcc catgcaaacg
      601 ccatgcataa cccttacgcc cgttttcatg aaccagtgac agtggaaggt tatttaaaag
      661 cacccatgat caccgacccc atcaacctga tggatgcttc tcccactggt gatggcgccg
      721 ccgcagccct ggttgtgcct atagagaaat tccaccaaaa caatgggcgc aaaatcatca
      781 gggtagcagc ttcttcagca gcaacggata cgatggctgt ccatcaccgc aaagacgctc
      841 ttttcctcaa agccgcatac aacgcagcga ccaaagcata cgcgcagtct tccctcacgc
      901 cgcaggatat tgaccttttt gaactccatg acgcgttttc gatcatggcg acgctttccc
      961 ttgaggccaa tggttttgca gaaaaaggga aaggccccca acttgcgctc gatggcgaga
     1021 tcctcaccaa tggcaggatc ccaattgcca ctcgcggcgg gttaaaagcc cgtggtcatc
     1081 cagtaggcgc cacaggtatg taccagattg ttgaagttgt agaacaactg cgcggcgaag
     1141 ctgctggaac ccaggtggaa aacgccaggg ttggcatgac acaaaacatc ggcggcagcg
     1201 gttcaaatat catttctcac atttttgcag taacagatta gttgcgtcgc tcaatacagc
     1261 tcctttcatg aaacctgata tgattggagc tggattgaga atggcaattg tttctgcttc
     1321 ccgtctgact gattaccggg cgctgacttt tcggttttcg ccaggctttt ccctctctac
     1381 ccctgaacag gcagttgaat atgtgaaaga acggggtttt atcttttttt ggcctgtgaa
     1441 aggtttcacc ttccccagcc tttggggagc ggtagcaggt gacaggccag tcaccgacga
     1501 gcacgatgat cccgggcata tcacctggca ctggaaggac tctctcctcg accaaagagt
     1561 ctggtattac gccagggtgc tcaggcgcaa gaacacgctt atctcattgg acgttctaaa
     1621 atacttttat gcgctttcgc ccaattatgg cgaaccggaa ctggacctta tagaccagta
     1681 caaccagggg gagttgcccc tggaagcaaa actgattttc gaagcgctca ataaaaatgg
     1741 tcccctggat acactctccc ttaggaaaga agcccggctt gctggtgtca attcacaatc
     1801 cccgttcaac cgcgcgctgg atatccttca ggctcagctt cgcgttctgc ccgtggcggt
     1861 aagcgaagcc ggcgcctgga agtacgcttt tgtgtatgac ctgacgcacc gttattaccc
     1921 gcaactctct gaaagcgcgc ggttcatcac cgaaaaccag gcgatggatt acctgttgcg
     1981 cctgtttttc aaatcgcttg gtgtagccag tttacgccag ttaacccata ctttcaggtg
     2041 ggatgctggg ttgaccatca aggccatcga acggatgatt caagaaggat atttgatgaa
     2101 agatgtgaag gttgaaggca gccctgaaaa ccattactgc ctggttgaat tattcgaagc
     2161 ataaattgat ttatgaaagc aacgcgttat tgcagctgtg tatataattc agctatctca
     2221 attgattgag aacggagcgc aggtatgga
//
LOCUS       DAOTLW010000108         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_40630_length_2174_cov_7.536102, whole genome shotgun sequence.
ACCESSION   DAOTLW010000108 DAOTLW010000000
VERSION     DAOTLW010000108.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40630_length_2174_cov_7.536102"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="P5516_08910"
     CDS             <1..641
                     /locus_tag="P5516_08910"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HRT92585.1"
                     /translation="NRALEVGLDPWEKPLREAETRGVYQQAVLTKASEILYPDAYFAS
                     AVSNSVLEHIPALEPVFAELGRVLRPGAVFVFCVPNQRFLGALSIGRFFDNLRLRFLG
                     DLYRRFFNRISRHHHCDDPSTWRQRLEGAGFELVDCWDYFSPRALRVLEWGHYFGLPS
                     LLTRLLWGRWIVAPWKWSLFFTRLITIKAYNEASIQAEGVYTFYITRKKIEN"
     gene            641..>2174
                     /locus_tag="P5516_08915"
     CDS             641..>2174
                     /locus_tag="P5516_08915"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRT92586.1"
                     /translation="MMDEPSLLDYLKSQLNLHRLFRGEKSPLVASDLPSAEEEEPEPI
                     KRRFILASLPWRSLLALGLALTGQRFFEPGATLPRLGIGFYLVAGGLLVAALLKGEWT
                     TPEAKRFNQPLSAATARKTPLFIFLPLLVITFLAFGGNRFTTGGLVLWAATLITGLWA
                     FWQREENGSVWAKIRQQTAGFFRGGHLLLQLNWWHLLVLAVFILSAWFHLNQLGTVPL
                     EMTSDHAEKLLDVNDILHGNYSIFFPRNSGREPLQFYLSAALVKYFGQPLGFTTLKLG
                     MALAFLGSLYYVYRLGGEVGSRWTGLLAMLLVGFSSWANILARSGMRLVLTPVFVAPV
                     LFYLLRGLRLSRRNDLLVAGIFLGFGLLGYSAFRIMPLVVLLIFLVFTLTYRQPKQKE
                     GIAAGLALVTLFAVVGALPLLRFAFQYPELIAMRTVTRMTGAEQALAGPLGVIFLQNL
                     WNAILMPFWRDGNTWVISVTGRPALDLVSASLYLSGAVILLFAWFKQRAWQYLALLFS
                     IPL"
BASE COUNT          386 a          578 c          632 g          578 t
ORIGIN      
        1 ttaaccgtgc gcttgaggtt ggcctggacc cctgggagaa gcctttacgt gaagccgaaa
       61 cgcgcggtgt gtatcagcag gcggtactga cgaaagcgag cgagatcctg taccctgatg
      121 cgtattttgc cagcgcggtg agcaattccg tgctggagca tatcccggcg cttgaacctg
      181 tgttcgcgga actggggagg gtgttacgcc cgggagcggt gttcgttttt tgtgtaccca
      241 atcagcgctt tttgggtgcg ctctccattg gccgtttctt cgataacctg cgtctgcgat
      301 ttttaggtga cctttaccgg cgttttttca accgcatttc gcggcatcat cactgtgatg
      361 acccctctac ctggcgacaa cgcctggaag gtgcagggtt tgagctggtg gactgctggg
      421 attatttctc cccgcgcgcg ttgcgcgtgc tggaatgggg acattatttc ggtctaccat
      481 cgctgctcac ccgcctgtta tggggcaggt ggatagttgc gccctggaaa tggagcctgt
      541 tcttcacccg tttgatcacc ataaaagcgt acaacgaagc ctccatacag gcagagggtg
      601 tgtacacctt ttatataacg cggaagaaaa ttgagaattg atgatggatg aacccagcct
      661 tctggactac ctgaaaagcc aattgaactt acaccgcctg ttcaggggcg agaaaagccc
      721 gctggttgcg tctgacctgc ccagcgcgga ggaggaggag cctgagccaa taaagcgcag
      781 gttcatcctg gcgtcgctgc cgtggcgctc actgctggcg ctgggcctgg ctttgaccgg
      841 tcaacgtttc tttgagccgg gagcgacact gcccagactg gggattggtt tctatctggt
      901 tgcaggaggg ttgcttgtcg cggcactgct gaaaggcgag tggacgacgc cggaagcgaa
      961 gcgtttcaat caaccccttt cggctgccac tgcgcggaag acacctctgt tcatcttctt
     1021 gcctttgctg gttatcacct ttttggcttt tggcggcaac cgttttacca ctggcggact
     1081 ggtgttgtgg gcggctacgc tgattaccgg gttatgggct ttctggcagc gcgaagaaaa
     1141 tggatctgtt tgggcaaaaa tcaggcaaca aaccgcgggc tttttccggg gaggtcatct
     1201 cctgctgcaa ctgaactggt ggcacctgct cgtgctggcg gttttcattc tcagcgcctg
     1261 gttccacctc aaccagctcg gcacggttcc attggaaatg accagcgacc acgcggaaaa
     1321 gctgctggat gtcaacgaca tcctgcatgg caattactcg attttctttc cccgtaattc
     1381 aggccgtgaa ccgctgcagt tttacctcag cgctgccctg gtaaagtatt tcggtcagcc
     1441 gctgggcttt accacgctca agctgggaat ggcattggcg ttcttgggct cactttatta
     1501 tgtctaccgc ctgggaggtg aagtagggtc ccgatggacc ggcctgctgg ccatgctgct
     1561 ggtgggtttt tcatcgtggg ccaatatcct tgcgcgttca gggatgcgcc tggtgctgac
     1621 gccagttttt gttgcgccag tgttattcta tttactgagg gggttgcgcc tttcgcggcg
     1681 caacgacctg ttggtcgcgg gtattttcct gggttttgga ttgctgggtt attctgcttt
     1741 ccgcatcatg ccgctggtgg tcctgttgat tttcctggtt ttcacgctca cttatcgcca
     1801 accgaagcaa aaagaaggca tagccgccgg gctggctttg gtaaccctgt tcgccgttgt
     1861 gggcgcgctc ccgctgctgc gcttcgcctt ccagtaccca gagctcatcg ccatgcgcac
     1921 cgttacgcgc atgaccggcg ctgaacaggc gcttgccggg ccattaggag tgatattcct
     1981 gcaaaatcta tggaatgcca ttttgatgcc tttttggcgc gatggcaata cctgggtcat
     2041 atctgtaacc ggaagaccag cgctcgacct ggtttccgcc tctctttatt tatcaggggc
     2101 ggtgatatta ctatttgcat ggtttaagca acgcgcctgg cagtacctgg cgctgctgtt
     2161 ctctatcccg ctgc
//
LOCUS       DAOTLW010000109         2174 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_40631_length_2174_cov_6.610193, whole genome shotgun sequence.
ACCESSION   DAOTLW010000109 DAOTLW010000000
VERSION     DAOTLW010000109.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_40631_length_2174_cov_6.610193"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="P5516_08920"
     CDS             <1..641
                     /locus_tag="P5516_08920"
                     /inference="COORDINATES: protein motif:HMM:NF024880.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HRT92587.1"
                     /translation="NRALEVGLDPWEKPLREAEARGVYQQAVLTKASEIPYPDAYFAS
                     AVSNSVLEHIPALEPVLAELGRVLRPGAAFVFCVPNQRFLGALSIGRFFDNLRLRFLG
                     DLYRRFFNRISRHHHCDDPSTWRQRLEGAGFELVDCWDYFSPRALRVLEWGHYFGLPS
                     LLTRLLWGRWIVAPWKWSLFFTRLITLKAYNEASIQAEGVYTFYITRKKIEN"
     gene            644..>2174
                     /locus_tag="P5516_08925"
     CDS             644..>2174
                     /locus_tag="P5516_08925"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HRT92588.1"
                     /translation="MDEPSLLDYLKSQLNLSRLFRGEQNPLVAPDLPSAEEEEPEPTK
                     RKFILASLPWRSLLALGLALSGQRFFEPGAALPRLGIGFYLVAGGLLVAALLKGEWTT
                     PEAKCFNQPLWAATARKTPLFIFLPLLVITFLAFGGNRFTTGGLVLWAATLITGLWAF
                     WQREENGSVWAKFRQKTAGFFRGGRLLLQLNWWHLLVLAVFILSAWFHLSQLSTVPLE
                     MTSDHAEKLLDINDVLHGNYSIFFPRNSGREPLQFYLSAALVKYFGQPLGFTTLKLGM
                     ALAFLGSLYYVYRLGGEVGSRWTGLLAMLLVGFSSWANILARSGMRLVLTPVFVAPVL
                     FYLLRGLRLSRRNDLLVAGNFLGFGLLGYSAFRIMPLVVLLVFLVFTLTYRQPKQKEG
                     IAAGLALVTLFAVVGALPLLRFAFQYPELIAMRTVTRMTGAEQAIAGPVGVIFLQNLW
                     NAILMPFWRDGNTWVISVTGRPALDLVSASLYISGMVILLFAWFKQRAWQYLALLFSI
                     PL"
BASE COUNT          386 a          567 c          634 g          587 t
ORIGIN      
        1 ttaaccgtgc gcttgaggtt ggcctggacc cctgggagaa gcctttacgt gaagctgaag
       61 cgcgaggtgt gtatcagcag gcggtactga cgaaagccag cgagatcccg taccctgatg
      121 cgtattttgc cagtgcggtg agcaattccg tgctggagca tatcccggcg cttgaacctg
      181 tgctcgcgga actggggagg gtgttacgcc cgggagcggc gttcgttttt tgtgtgccca
      241 atcagcgctt tttgggtgcg ctttccattg gccgtttctt cgataacctg cgcctgcgat
      301 ttttaggtga cctttaccgg cgttttttta accgcatttc acggcatcat cactgtgatg
      361 atccctctac ctggcgacaa cgcctggaag gtgcagggtt tgagctggtg gactgctggg
      421 attatttctc cccgcgcgcg ttgcgcgtgc tggaatgggg acattatttc ggtctaccat
      481 cgctgctcac ccgcctgtta tggggcaggt ggatagttgc gccctggaaa tggagcctgt
      541 tcttcacccg tttgatcacc ttaaaagcgt acaacgaagc ctccatacaa gcagagggtg
      601 tgtacacttt ttatataacg cggaagaaaa ttgagaattg aagatggatg aacccagcct
      661 tctggactac ctgaaaagcc aattgaattt aagccgcctg ttcaggggcg agcaaaaccc
      721 gctggttgcg cctgacctgc ccagcgcgga ggaagaggag cctgagccga caaagcgcaa
      781 gttcatcctg gcgtcgctgc cgtggcgctc cctgctggcg ctgggcctgg ctttgagcgg
      841 tcaacgtttc tttgagccgg gagcggcact gcccaggctg gggattggtt tctacctggt
      901 cgcaggaggg ttgcttgtcg cggcactgct gaaaggcgag tggacgacgc cggaagcgaa
      961 gtgtttcaat caaccccttt gggctgccac tgcgcggaag acacctcttt tcatcttctt
     1021 gcctttacta gttatcacct ttttggcttt tggcggcaac cgctttacca ctggcggact
     1081 ggtgttgtgg gctgctacgt tgattaccgg gttgtgggct ttctggcagc gcgaagaaaa
     1141 tgggtctgtt tgggcaaaat tcaggcaaaa aaccgcgggc tttttccggg gaggtcgtct
     1201 cctgctgcaa ctgaactggt ggcacctgct cgtgctggcg gttttcatcc tcagcgcctg
     1261 gtttcacctc agccagctca gtacggttcc attggaaatg accagcgacc acgcagaaaa
     1321 gctgctggat atcaacgatg ttctgcatgg taattactcg attttctttc cccgtaattc
     1381 aggtcgtgaa ccgctgcagt tttacctcag cgctgcactg gtaaagtatt tcggtcagcc
     1441 gctgggcttt accacgctca agttgggaat ggcattggcg ttcttgggct cactttatta
     1501 tgtctaccgc ctgggaggtg aagtagggtc ccgatggacc ggcctgctgg ccatgctgct
     1561 ggtgggtttt tcatcgtggg ccaatatcct tgcgcgttca gggatgcgcc tggtgctgac
     1621 gccagttttt gttgcgccag tgttattcta tttactgagg gggttgcgcc tttcgcggcg
     1681 caacgacctg ttggtcgcgg gtaatttcct gggttttgga ttgctgggtt attctgcctt
     1741 ccgcattatg ccgctggtgg tcctgttggt cttcctggtt ttcacgctca cttatcgcca
     1801 accgaagcag aaagaaggca tagccgccgg gctggctttg gtaaccctgt tcgcggttgt
     1861 gggcgcgctc ccgctgctgc gcttcgcctt ccagtaccct gagctcattg ccatgcgcac
     1921 agttacgcgc atgaccggcg ctgaacaggc gatcgccggg ccagtaggag taatatttct
     1981 gcaaaatcta tggaatgcca ttttgatgcc tttctggcgt gatggcaaca cctgggtcat
     2041 ttctgtaacc ggaagaccag cgctcgacct ggtttccgct tctctttata tctcagggat
     2101 ggtgatatta ctatttgcct ggtttaagca acgcgcctgg cagtacctgg cgctgctgtt
     2161 ctctatcccg ctgc
//
LOCUS       DAOTLW010000110         2150 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_41229_length_2150_cov_7.717422, whole genome shotgun sequence.
ACCESSION   DAOTLW010000110 DAOTLW010000000
VERSION     DAOTLW010000110.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_41229_length_2150_cov_7.717422"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..947
                     /locus_tag="P5516_08930"
     CDS             <1..947
                     /locus_tag="P5516_08930"
                     /inference="COORDINATES: protein motif:HMM:NF012321.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HRT92589.1"
                     /translation="PEPSLQNPFNKNDPFWWAAGRKMPNGEEFNPRKLDTPLDKIMRT
                     SAGRRSVTRTERKRGRYVQSRPAQGKTDDLAFDATVRAAAPFQKERAEIKERHRVAFA
                     VKPADYMKKVRVRKAANLILFLVDASWSMAVAERMAATKGAILSLLTDAYQRRDRVGL
                     IVFQKNLATQVLSPTNSVMLAEAALKDIPVGGKTPLSAGLMMAYEVLRKEKILHPEVM
                     PLLIVLTDGAGNVSIGFSSPQEEAYHIADQIAKENIHSVVVNMEHAAFDQGLAQSLAD
                     HLKAPCYTITDLKAESLYVTVQQEIRQVNASSNIELKK"
     gene            1018..1413
                     /locus_tag="P5516_08935"
     CDS             1018..1413
                     /locus_tag="P5516_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92590.1"
                     /translation="MIQDPVKRTRVKSTLLIVIAATIPCYLLGMIVLWVSDGVRNRPT
                     ATPTVTEEMTITQSSSTEELPTLPVPSAQFPTATYTLTPSITPTLTVTRTYVIPSSTP
                     SLTPSMTETMTPSETLTTAPTPSETSSGP"
     gene            1450..>2150
                     /locus_tag="P5516_08940"
     CDS             1450..>2150
                     /locus_tag="P5516_08940"
                     /inference="COORDINATES: protein motif:HMM:NF017181.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92591.1"
                     /translation="MNQIESFLHQLLSAPGVAGFEGPVCEIIRKRWEPLTDEIHISRV
                     GSLHALRRSSIPGKRPSLMIAAHMDAIGLMVKDIRDGLIWITSMGGVDPRILPGQLVT
                     IHGVREYAGVVQMLPDRLVKHQPAGSAPGYSQLFVDTGLSANEIQANIHIGDVISFAS
                     APTTLGDKYLSGHSLDNRASVAALTVCLEEIKDYRLQWDLWAVATVQEEVRTVGAHTS
                     TFELKPDVGIAVDVT"
BASE COUNT          532 a          616 c          546 g          456 t
ORIGIN      
        1 aaccagaacc gagcctgcag aacccgttta acaagaacga tcctttttgg tgggccgccg
       61 gtaggaagat gccaaatggt gaggaattta atcctcgcaa acttgacacc ccgctggata
      121 aaatcatgcg cacatccgcc ggcaggcgct ctgtaacccg cactgagcgc aagcgcgggc
      181 gttatgtgca atcgcgccca gcgcagggaa aaacggatga tctggcattt gacgctactg
      241 tgcgcgcagc agcaccgttc cagaaagaac gcgctgaaat aaaagaacgc caccgcgtgg
      301 cctttgccgt gaaaccggca gattacatga aaaaagtgcg tgtccgcaaa gcagccaatc
      361 tcatcctttt tctggttgat gcttcatggt caatggcggt ggctgagcgc atggccgcca
      421 ccaaaggtgc cattctgtca ctgctgacag acgcttacca gcggcgcgac cgcgtgggct
      481 tgatcgtttt tcaaaagaac ctcgccacgc aggtgctttc tcccaccaac tcggtgatgc
      541 tggcagaagc cgcgctcaag gacatcccgg ttggcggtaa aactcctctc agcgcggggt
      601 tgatgatggc ttacgaagtg ctgcgcaagg aaaaaatcct gcatccagag gtgatgcctc
      661 tgcttatcgt gcttacagat ggcgccggca atgtttccat cggtttcagt tcaccgcaag
      721 aagaggcgta tcatatcgct gaccagattg ccaaagaaaa cattcatagt gtggtggtca
      781 atatggaaca cgccgctttc gaccaggggt tagcgcaatc actggcagac caccttaaag
      841 caccctgcta taccattact gaccttaaag cggaaagcct ttacgtcacc gtccagcagg
      901 aaatccgcca ggtgaacgcg agtagtaata tcgaactaaa gaaatgactg cttcaacact
      961 gtaaaaacgc ttgcccggtt gtaaggcacg tgataatatg aggggcagga accccttatg
     1021 atacaggacc cggtaaaacg cacacgcgtt aaatcaacac tgctcatcgt tatcgctgcc
     1081 accatcccct gttatttgtt gggaatgatc gttctgtggg taagcgatgg ggtgcgcaac
     1141 cgccccacag ccactccaac agtcaccgag gagatgacca tcacccagtc ctcctcaact
     1201 gaggagttgc ccacgctgcc agtcccctcg gcgcaatttc caaccgctac ctacacactc
     1261 acgccttcca ttacccccac gctcacggtc actcgtacct atgtgatacc atcatccact
     1321 cccagcctga ccccttcaat gactgaaacg atgacccctt cagaaacact aactaccgca
     1381 ccaacaccct ctgaaacctc cagtggccca taatcctcaa taccctttca ttaaaaaggt
     1441 agagtgtgaa tgaatcagat tgaatctttt ttacatcaat tactcagcgc tcctggcgtg
     1501 gcgggttttg aaggcccggt atgcgaaatc atccgcaaac ggtgggaacc cctaaccgac
     1561 gagatccaca tcagccgcgt ggggtctctt cacgctttgc gccggtcttc aatcccaggc
     1621 aagcgtccga gcctgatgat cgccgctcac atggacgcga tcgggctcat ggtgaaagac
     1681 attcgagatg gtttaatctg gatcaccagt atgggaggtg ttgacccgcg catactgccc
     1741 ggtcaactgg taaccattca tggagtcagg gaatacgccg gcgtggtgca aatgctgcct
     1801 gaccgcctgg tgaagcatca gccagcgggg agcgcccccg ggtattctca attgtttgtg
     1861 gacacaggct tgagtgcaaa cgagatccag gcgaacatcc acatcggtga tgtgatttcg
     1921 tttgctagcg ccccaaccac actgggcgac aaatacctta gcggtcacag cctggataac
     1981 cgcgcatctg ttgccgcttt gaccgtatgc ctagaagaga tcaaagacta ccggcttcag
     2041 tgggacctgt gggcggtggc aaccgtgcag gaggaggtgc gaactgtagg cgcgcacacc
     2101 tcgacgttcg agctcaaacc cgatgttggt atcgctgttg atgtgacctt
//
LOCUS       DAOTLW010000111         2061 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_43694_length_2061_cov_10.426221, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000111 DAOTLW010000000
VERSION     DAOTLW010000111.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2061)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2061
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_43694_length_2061_cov_10.426221"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..716)
                     /locus_tag="P5516_08945"
     CDS             complement(<1..716)
                     /locus_tag="P5516_08945"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.13.5"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_function: GO:0033890 - ribonuclease D activity
                     [Evidence IEA];
                     GO_process: GO:0042780 - tRNA 3'-end processing [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease D"
                     /protein_id="HRT92592.1"
                     /translation="MFLLLLNRMIIPAQRFVQGLGNPMQYNDDMMMPEMPAPIVVEDD
                     ESFAHMCADLAAQPAIAVDTESNSLHAYQERVCLIQISTRAQDYLVDTLALDSLDLLG
                     EVFANPAIQKVFHAGDYDLTCLKRDYAFTFTNYFDTSIAAAAVGEENLGLSTLLTKYF
                     GFSVDKRYQRANWGKRPLDPEMLRYAQSDSHFLLPLRDCLLPRLEATGRLNLVLEDCA
                     ALAQQTPPMKNHSEDVWRVKG"
     gene            715..1182
                     /gene="dtd"
                     /locus_tag="P5516_08950"
     CDS             715..1182
                     /gene="dtd"
                     /locus_tag="P5516_08950"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015293283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HRT92593.1"
                     /translation="MRAVIQRVSQAQVRVENKVVAKIGLGLVVLLGISPTDTEADVRE
                     MAEKVANLRIFGDAQGKMNLSCLDVNGEIIVVSQFTLYADTRRGRRPSFTGAASPELA
                     EPLCQLFVETLRIMGVPTQSGVFGAHMVVDIQNDGPVTILLEQPGDSRSSWNF"
     gene            complement(1179..1643)
                     /locus_tag="P5516_08955"
     CDS             complement(1179..1643)
                     /locus_tag="P5516_08955"
                     /inference="COORDINATES: protein motif:HMM:NF016408.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HRT92594.1"
                     /translation="MLGKKKSPPTFDVEPAAVRVTSVLGPGINWKGDLRGKGGVRIEG
                     TMEGEIAIQGLVVIGETGKVTCQMLQAETVIVAGAVKGNIRCGKLEIRATGRVWGDVV
                     TTALSSEEGAFLRGQVRMEERVDVPPVDATPEAEVSDNFSTEAQPSADQPPA"
     gene            complement(1673..>2061)
                     /locus_tag="P5516_08960"
     CDS             complement(1673..>2061)
                     /locus_tag="P5516_08960"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF014316.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HRT92595.1"
                     /translation="HWLLNAVTDKEGVPAAVLIRGLLPTEGLEKMEELRPTLDRENGW
                     LNGPAKLAQALALDGQLNRADLCAPDSSLFIEVGVEVPSKSLLVKPRVGINSVPEPWR
                     SVPWRWQIDPQTLQKLSSDLRIASDL"
BASE COUNT          456 a          565 c          542 g          498 t
ORIGIN      
        1 ccctttacgc gccagacgtc ttcgctatgg tttttcattg gcggcgtttg ctgagccaaa
       61 gcagcacaat cctcgagcac caggttcagc cgtcccgtgg cttctaagcg cggcagcaag
      121 caatcgcgta agggcagcaa gaagtgcgaa tcggattggg catagcgtag catttccgga
      181 tcgagcgggc gtttgcccca gttggcgcgc tggtatcttt tatcaacgct gaagccaaaa
      241 tattttgtca atagggttga taaacctaaa ttttcctcgc ccaccgcagc agctgcgatg
      301 ctcgtatcga aataattagt aaaagtaaag gcgtaatcgc gcttgaggca agtgagatcg
      361 taatcgccag cgtgaaagac tttttggatg gctgggttgg caaagacttc gcctagtagg
      421 tcgagggaat caagcgcaag tgtatccacg aggtaatcct gcgcgcgagt ggagatttga
      481 atcaggcaga cgcgttcctg ataagcatgc aagctgttag attcagtatc gactgcgata
      541 gctggctgag cagctaaatc agcgcacatg tgcgcaaagc tctcatcgtc ctccaccact
      601 atgggagctg gcatttcagg catcatcata tcgtcattat actgcatcgg gttacccagc
      661 ccttggacga agcgctgagc gggtataatc atcctattca aaaggagtaa gaacatgcgt
      721 gcagtcattc aacgggtaag ccaagcgcaa gtgcgggttg aaaataaagt cgttgctaaa
      781 atcggcttag gcttggtggt gctgctaggc attagtccaa ctgataccga agccgacgtg
      841 cgcgagatgg ctgaaaaagt ggctaatctg cgcattttcg gggatgcgca gggcaaaatg
      901 aacctctcct gcttggatgt gaatggggag atcatcgtgg tttcccagtt tacgctctac
      961 gcggatacgc gccgtggtcg ccgtccttcc ttcacgggag ctgccagccc tgagctggct
     1021 gagccgcttt gtcagctttt tgtggagacg ctgcgcatca tgggcgtgcc cacccaaagc
     1081 ggcgtctttg gcgcccacat ggtggtcgat attcaaaatg acgggccggt cacgatcctg
     1141 ctcgagcagc ccggcgattc acgttcttcc tggaatttct aagcaggtgg ctggtcagcg
     1201 cttggctgcg cttcagttga aaagttatcc gaaacttcgg cttcaggggt tgcgtcgact
     1261 ggtggcacat caacccgctc ttccatgcgc acctgcccgc gtaagaatgc cccctcttca
     1321 ctgctgagcg cagtcgtaac cacatcaccc cacacacgcc ccgtagcacg gatttcgagc
     1381 ttaccacagc ggatattacc ctttaccgcg ccagctacga tcaccgtttc agcttgcagc
     1441 atttggcagg tcactttacc cgtctcgccg ataactacca aaccttgaat ggcaatctcg
     1501 ccttccatgg taccttcgat gcgtacgccg cctttgccac gcaagtctcc cttccagttg
     1561 atgcctggtc caagtactga ggtaacgcgt acggcagcgg gttccacgtc aaaagttggc
     1621 ggcgatttct tttttcctaa cataattagt gcatttttcc tttcggcgtc aattataaat
     1681 cactcgctat ccgtaaatcg ctgctcaact tctgtagtgt ttgcgggtcg atttgccagc
     1741 gccatggcac cgagcgccag ggctctggga ctgaatttat ccctacgcga ggtttaacca
     1801 gcaaggactt agatggaacc tccacaccca cttcgataaa aaggctcgaa tccggcgcac
     1861 acaggtcagc ccggttcagc tgtccatcca gcgctaaggc ttgagccagt ttggcgggtc
     1921 cattgagcca tccattttcg cgatcgagcg tcggacgcag ttcttccatc ttttccagcc
     1981 cttcagtggg cagtagccca cggatgagca ctgcggctgg taccccctct ttatcagtaa
     2041 cggcattgag cagccagtgc a
//
LOCUS       DAOTLW010000112         2049 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_44054_length_2049_cov_3.039619, whole genome shotgun sequence.
ACCESSION   DAOTLW010000112 DAOTLW010000000
VERSION     DAOTLW010000112.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44054_length_2049_cov_3.039619"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(373..660)
                     /locus_tag="P5516_08965"
     CDS             complement(373..660)
                     /locus_tag="P5516_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92596.1"
                     /translation="MPKPKNEFPSVRVRLDGYLQMREEGVTADPRQQALYNFLVEHRS
                     DRKAFPMAIELLTAALNGELGEQVKTAVEAGDTQAAVEAARDLISEFVIDI"
     gene            complement(660..860)
                     /locus_tag="P5516_08970"
     CDS             complement(660..860)
                     /locus_tag="P5516_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92597.1"
                     /translation="MNDKSPDECTVAELREDFLKHVHEAPKFFIWWMPVYVWLLARTW
                     WRLKFWFDYHFDLPFRKPNGDC"
     gene            complement(937..1935)
                     /locus_tag="P5516_08975"
     CDS             complement(937..1935)
                     /locus_tag="P5516_08975"
                     /inference="COORDINATES: protein motif:HMM:NF037161.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParM/StbA family protein"
                     /protein_id="HRT92598.1"
                     /translation="MVTKLGVDFGMSSSKIVGEKGEVMFFNQVAIPLGGKVEGSFTGM
                     KARRRPMVMSGAFGEFYVGKNAHDFGQEVESFDFDTLTGTNEMRALFYGALTAFQKQF
                     GRFEGGLELAVMLPLQLITGERAKKNQDAVKGWLVGSHEWTADGEAYAVTVDKIALAP
                     QAIGALFDYVFDMAGQAIAGHEDAMKLECAEISVGSNSVELMVTKRNSDTEKFNGGKA
                     VGVRKLFKRIDPRGLYTYGELDMKLRGKDDEFSDEIKAALPGHVDSWAEEVKGYINKQ
                     WEGAHERFHKIFLVGGGVHLIEKHLRQRFNGRTVVPENPLMSIARGGYKILLVNGK"
BASE COUNT          542 a          717 c          347 g          443 t
ORIGIN      
        1 ccacccccaa agaccgtcgc gccgcatccg gcaccacacc caacaacggc accacatcca
       61 ccgcctcacc acccctccgc accaacatcc cccgcatccc cgtataaggg ttcacatcat
      121 caaaaactac agttggttca ttcataaaat cactcacttt cgaaaccgct tcgtagtata
      181 aaaaccacac cacaaaccag ccttactcaa ccaatcgaca atccaaaatc aaaaatcgta
      241 aattgccatt cccctccccc atcacccagg gatgtcccgt atcgcatcca cgcctcgcgc
      301 acgcacacaa atcatcatgg ggtgggggag ttacaatctg gcggttaaat acctaacacg
      361 cataattggc ggttaaatat caatcacaaa ctcactgatc agatctcgcg ctgcttcgac
      421 cgctgcttgc gtatcgccag cctccacagc cgtcttcacc tgctcgccca gctccccatt
      481 cagcgccgcc gtcaacaact cgatcgccat cgggaacgcc ttgcggtcac tacgatgctc
      541 caccaaaaag ttatacaacg cctgctgccg tggatccgca gtcacaccct cttcacgcat
      601 ctgcaaatac ccatccaacc gcacccgcac actcggaaat tcattcttag gttttggcat
      661 cagcaatctc catttggctt acgaaacggt aaatcgaagt ggtagtcaaa ccaaaatttc
      721 aaacgccacc acgttcgtgc taacagccat acatagactg gcatccacca aataaaaaat
      781 tttggtgctt catgaacgtg tttcaaaaag tcttcgcgta attcagccac agtgcattca
      841 tcaggtgatt tatcgttcat cgttttcctc tctcccccca aatatccgcg aagcggtatt
      901 tggggggatg tcgcttcagc gacagggggg taggggtcac ttcccattca ccagcaaaat
      961 cttataccca ccccgcgcaa tactcatcag cgggttttcc ggcaccaccg tccgcccatt
     1021 gaagcgctgg cgcaaatgct tctcgatcaa atgcacccca ccgcccacca ggaagatctt
     1081 atgaaaacgc tcatgcgcac cttcccactg cttgttgata taccccttca cttcttcagc
     1141 ccacgagtca acatgcccag gcaaagccgc cttgatctca tcgctgaatt catcgtcctt
     1201 accgcgcagc ttcatatcca actcgccata ggtatacaac ccacgcgggt caatccgctt
     1261 gaacaactta cgtacaccca cagccttccc gccgttgaac ttctccgtgt ccgaattgcg
     1321 cttcgtcacc atcaactcca ccgaattcga acccacagag atctccgcac attccagctt
     1381 catcgcatct tcatgcccgg caatcgcttg cccagccata tcgaacacat aatcgaacag
     1441 cgcaccaata gcctgcggag ccagcgcgat cttatcgacc gtcacagcat acgcctcacc
     1501 atccgccgtc cactcgtgcg aaccaaccag ccaccccttc accgcatcct gattcttctt
     1561 cgcccgctcc ccagtgatca actgcaacgg cagcatcaca gccaattcca acccgccttc
     1621 aaaacgccca aactgcttct gaaacgccgt caacgcccca tagaacaacg cccgcatctc
     1681 attcgtgccc gtcaaagtgt caaagtcaaa gctctccact tcctgaccaa aatcatgcgc
     1741 gttctttcca acatagaact cgccaaaagc accgctcatc accatcgggc ggcggcgcgc
     1801 cttcatccca gtaaacgaac cttccacctt cccccccaaa ggaattgcca cctggttgaa
     1861 gaacataacc tcgcccttct cacccacaat cttcgacgaa ctcataccaa aatccacacc
     1921 aagtttcgtt accatgctat ttattctcct tttcgatgtt ttcaaaattt tcttctcggc
     1981 agcaggtggt cagacttata aaaatcaaga taagtaagaa ccgccatcaa caatgccacc
     2041 ggaatgaat
//
LOCUS       DAOTLW010000113         2026 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_44708_length_2026_cov_7.340436, whole genome shotgun sequence.
ACCESSION   DAOTLW010000113 DAOTLW010000000
VERSION     DAOTLW010000113.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2026)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2026
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44708_length_2026_cov_7.340436"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="P5516_08980"
     CDS             <1..419
                     /locus_tag="P5516_08980"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92599.1"
                     /translation="VVPLLVVMLITQTTIHLTFLFLPISILLLTAFALGFGLLLSALA
                     IFFPDVAEMYQILLMAWMYLTPVIYPEEIIPAQFLTIYRINPMYWMLKMFRMPVYEGV
                     IPSLQDLWPALAWSVGMLIVGWLFFTSRSDEYAYRV"
     gene            397..1185
                     /locus_tag="P5516_08985"
     CDS             397..1185
                     /locus_tag="P5516_08985"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92600.1"
                     /translation="MNMLIESKPLLSQKAKLNSDPVITLQDVSVAYRVPKERIGTFKE
                     YAIRTLQRKVQFNKFLALKDVSVTINRGEVFGIIGNNGAGKSTMLKVISRVLRPTKGR
                     VLLYGTIAPLLELGAGFHPELTGRENVYLNGALLGYDQDEMDTVFEEIVEFSELREFI
                     DAPLRTYSSGMYARLGFAVATAHVPEILIVDEILSVGDEAFQRKCNERMQSYQEQGST
                     VLIVSHGLAKIQDMCNRVLWLDHGQVQMIGDPAEVIEAYRRSNQ"
     gene            1187..1765
                     /gene="rfbC"
                     /locus_tag="P5516_08990"
     CDS             1187..1765
                     /gene="rfbC"
                     /locus_tag="P5516_08990"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRT92601.1"
                     /translation="MEFIPTTIPDVVLIIPRIFGDERGFFLETYRQDVFESACGPINF
                     VQDNHSGSRQGTLRGLHYQLHQAQGKLVRAVAGEIFDVAVDIRQGSPTFGQWVGARLS
                     AENKHQLWVPAGFAHGFYVLSPWAEVFYKASAYYAPEWERGIRWDDPAIGIAWPLAEG
                     GTPLLSDKDQRNPTLAEAETYPVGWQPEQEEE"
     gene            1768..>2026
                     /locus_tag="P5516_08995"
     CDS             1768..>2026
                     /locus_tag="P5516_08995"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HRT92602.1"
                     /translation="MRHILVTGGAGFIGSNFVHYLLRSEPDVQIINLDALTYAGSLEN
                     LKDLPDPSRHVFIHGDIANRELVDRIFAEYGIDTVVHFAAET"
BASE COUNT          459 a          508 c          547 g          512 t
ORIGIN      
        1 ccgtggtgcc gctgctggtg gtcatgttga tcacgcagac aaccattcac ctgacatttc
       61 tctttcttcc catttcaatc ttgctgctca ccgcatttgc gctcgggttc gggttactgc
      121 tttcagcttt ggcgatattc ttcccagatg tggctgaaat gtatcagatc ctgctgatgg
      181 cttggatgta cctcacaccg gttatttacc ctgaagaaat catcccagcg cagttcctga
      241 ccatctaccg catcaacccg atgtattgga tgctcaaaat gttccgtatg cccgtttatg
      301 aaggggtaat cccaagtttg caggaccttt ggccagcgtt agcttggtca gtgggtatgc
      361 tgattgtggg ttggctattt ttcacctcga gatcggatga atatgcttat cgagtctaaa
      421 ccattgctga gccaaaaagc caagctgaac agcgatcccg tcatcacact gcaggatgtc
      481 tcggttgcat accgtgttcc taaagaacgg atcggaactt tcaaagaata tgccatccgt
      541 acgctgcaac gtaaggttca attcaacaag tttttagcgc tcaaagacgt cagcgtgacg
      601 attaaccgtg gagaggtctt tggcatcatc ggtaataacg gcgccggaaa aagcaccatg
      661 ctgaaagtga tctcacgcgt cttacgcccc accaagggac gcgtgctgct ttatggcacc
      721 atcgcacccc tactggaatt gggcgccggc tttcaccccg agctcaccgg gcgggaaaat
      781 gtttatctga atggggctct gctgggttac gaccaggatg agatggacac cgtgtttgaa
      841 gagatcgttg aattttcaga gctgcgtgag tttattgacg ctccattgcg cacctattcc
      901 tcaggtatgt acgcgcgctt aggttttgct gtggcgacag cgcatgtgcc agagatcctc
      961 attgtggatg aaatcctcag cgtcggggat gaagcctttc aacggaagtg taatgagcgt
     1021 atgcaaagtt atcaagaaca aggcagcacg gttttaattg tttcgcatgg tttggcaaag
     1081 attcaggata tgtgtaaccg tgtgctctgg ctggatcacg ggcaagtgca gatgattggc
     1141 gatccagctg aagttatcga agcttaccgc aggagcaacc aataaaatgg aatttatccc
     1201 taccactatc ccggatgttg tgctgattat tccccgcatc ttcggggatg agcgcggctt
     1261 tttcttagag acgtatcggc aggatgtttt tgagtctgcc tgcggtccga ttaacttcgt
     1321 gcaagataat cattcgggtt caaggcaggg caccctgcgt gggctgcact accagctgca
     1381 ccaagcccag ggtaaattag tacgcgccgt ggcgggagag atcttcgacg tcgccgtcga
     1441 tatccgccag ggctcgccaa cctttggaca atgggtcggg gcgcggcttt ctgccgaaaa
     1501 caaacatcaa ctctgggtgc cggcaggttt tgcgcacggg ttctatgtgc tcagcccttg
     1561 ggcagaggtg ttctataaag ccagtgctta ttacgctccg gagtgggagc gcggcatccg
     1621 ctgggatgac cccgccatcg gcattgcttg gccgcttgcg gaaggcggta cgccgctgct
     1681 ttcggataaa gaccaacgca acccaacatt ggctgaagca gaaacttatc ccgttggctg
     1741 gcagccagag caagaggagg aataattatg cggcatatat tggtcacagg cggggctggc
     1801 tttatcggct caaattttgt gcattacctg ctgcgcagcg aaccggatgt acagatcatc
     1861 aacttagatg cactcaccta tgctggcagc ctggagaacc tcaaagacct acctgacccg
     1921 agccggcatg tatttatcca cggggatatc gcgaatcgag agttggtcga tcgcatcttt
     1981 gcggaatacg ggattgatac cgtggtgcac tttgccgccg aaacgc
//
LOCUS       DAOTLW010000114         2024 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_44771_length_2024_cov_9.531742, whole genome shotgun sequence.
ACCESSION   DAOTLW010000114 DAOTLW010000000
VERSION     DAOTLW010000114.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 2024)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2024
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_44771_length_2024_cov_9.531742"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="P5516_09000"
     CDS             complement(<1..297)
                     /locus_tag="P5516_09000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92603.1"
                     /translation="MSNPMSEAERSAAWQELQGFARALQEFNAPQIRAAFVIGSFPGG
                     YFRPGQSDLDVVIIFAGSPPTDEPTCQQHQALRQAIKELASKASPYEIEPMFIYE"
     gene            complement(294..1730)
                     /locus_tag="P5516_09005"
     CDS             complement(294..1730)
                     /locus_tag="P5516_09005"
                     /inference="COORDINATES: protein motif:HMM:NF019310.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HRT92604.1"
                     /translation="MQKPLRWYDSLTINIYFFGLTLRAQTLTPLILPLLVQQFVGDAN
                     KGASYGNIRLYSLMIAVLVQALAGMFSDRSTSRWGKRRPFMLASSLLESITFIAIGII
                     AARMEGQAGYNALFAAVIASMVFSNIGQGAAQGLIPDLVPPEKQGRFAGIKALLELPL
                     PLIFMAFFVSRMIAAGNLWGAITTVIITLLVCTAIAMLVRETPQEKAPYPLDWKAIGR
                     LIAMTAAFTLVILFSGWAVQQLIPLVSNLQLGSSPALTILIGLIGMLIAVVVGVLVSL
                     RLSLGKESQEKPAFTWWVINRLAFLVAASNLASFLLYFLQERFPGLQGNAAAQPATLL
                     VMATGIAILLAALPAGWLADKIGTKPVLAVSGIIAAAGTLVVIAAQDMSTMTVGAVLV
                     GVASGAFYSANWALGTQIVPKKEAGRYLGISNLAGAGAGAIGAYIGGPIGDSSGYVVQ
                     MAIYAALFLFSTLALLRIKPANHKAEHA"
     gene            complement(1765..>2024)
                     /locus_tag="P5516_09010"
     CDS             complement(1765..>2024)
                     /locus_tag="P5516_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HRT92605.1"
                     /translation="DKYLTHQNVLIVDDVWGSGRTITAVKNRVAAAGGHPSTCVLHFN
                     PYRNLFGNQRPNYYGAITDAHIVYPWESDRGFQSRFITNIP"
BASE COUNT          521 a          517 c          572 g          414 t
ORIGIN      
        1 ttcgtaaata aacataggct caatctcata aggagaggct ttgctggcaa gctccttgat
       61 tgcctggcgt aaggcttgat gttgttggca ggtaggttca tctgttgggg gtgaacctgc
      121 aaaaattatg accacatcca ggtcgctttg cccagggcgg aagtagccgc ccgggaaaga
      181 gccgatcaca aaagctgcac ggatctgcgg cgcgttgaac tcctggagtg cgcgagcaaa
      241 cccctgcagt tcctgccagg cagcgctgcg ctcagcttcg ctcatcgggt tgctcatgcg
      301 tgctctgcct tgtgattagc gggtttgatg cgcaaaaggg cgagcgtgga gaagagaaat
      361 agcgcggcat aaatcgccat ctgaacgaca taccctgagg agtccccgat gggaccgccg
      421 atataagcgc cgatggcacc tgcgccggca cctgccaagt tagagatgcc caagtaacgg
      481 ccagcctcct ttttggggac gatctgcgta ccgagcgccc agttggcgga gtaaaaagca
      541 ccgctggcta cccccaccag aactgcccct acggtcatcg tggacatatc ctgcgcggcg
      601 atgaccacga gcgtgccggc ggcagcgatg atgccgctaa cagccagcac tggtttggtg
      661 ccgattttat cggctaacca gccggctggt aatgctgcga gtaaaatggc aatgcctgtt
      721 gccatcacga gtaaggttgc tggttgggca gcagcatttc cttgtaaacc agggaagcgc
      781 tcttgtaaaa agtaaagcaa gaaactggct aagttgctgg cagccaccaa aaaagctaag
      841 cggttgatca cccaccacgt gaaggcgggt ttttcttggc tctctttgcc taagctaagg
      901 cgtaaactaa ccaagacgcc caccactaca gcaataagca taccgatgag accaatcaag
      961 atggtaagag ccggtgatga gccaagttgg aggttgctca ccaatggtat tagctgctgg
     1021 acagcccaac ccgaaaacaa gataaccaga gtaaaagctg cggtcatggc aatcagccga
     1081 ccaatagctt tccagtctag ggggtatggg gctttttctt gaggggtttc gcgcaccagc
     1141 atggcaatcg cggtgcaaac cagcaaggtg ataatcacgg ttgtgatagc gccccacagg
     1201 ttgccggcgg caatcatccg cgagacgaaa aaagccataa agatcaaggg caagggcagc
     1261 tcgagcaggg ctttaatgcc cgcaaaacgc ccttgcttct caggcggcac caagtcaggg
     1321 atcaacccct gagcagcacc ttgcccaatg ttcgaaaaca ccatcgaggc gatcacggcg
     1381 gcaaacaagg cgttatagcc agcctgccct tccatacgtg cggcaatgat gccaatcgca
     1441 ataaaagtga tcgattcgag cagcgagctg gcaagcataa acggtctgcg tttgccccaa
     1501 cgagaagtgc tgcggtcgga aaacatcccg gctaaggctt gcaccagcac agcgatcatt
     1561 aaagaataga ggcggatgtt accataactg gcacctttat tggcgtcccc tacaaactgc
     1621 tgcaccaaga gcggcagaat gaggggcgtc agcgtttggg cacgcagggt gagaccaaag
     1681 aagtaaatgt tgatggtgag gctgtcgtac caacgcaagg gtttttgcat atcttctcct
     1741 ggaaatacag atcgggctta aggcttaggg aatattggta ataaagcggg attgaaaacc
     1801 acgatcactt tcccaagggt aaacgatatg cgcatcggta attgcgccgt agtagtttgg
     1861 gcgctgattg ccaaataagt tgcggtaggg gttaaagtgc aatacgcagg tggatggatg
     1921 cccgccggca gctgctacgc ggttcttcac cgcggtgatg gtgcgccccg agccccaaac
     1981 atcatccaca atgagtacat tttggtgggt taagtattta tcat
//
LOCUS       DAOTLW010000115         1865 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_50100_length_1865_cov_35.525967, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000115 DAOTLW010000000
VERSION     DAOTLW010000115.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1865)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1865
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50100_length_1865_cov_35.525967"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="P5516_09015"
     CDS             <1..272
                     /locus_tag="P5516_09015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92606.1"
                     /translation="NPQTSASPRLSVSALKLSLPHANSLTPNAERRTQNAERRSAGRG
                     KRLLALAMKAGRSRRDRRRLDEQTLVPPGLQRSEISGQRRKTIHD"
     gene            393..956
                     /locus_tag="P5516_09020"
     CDS             393..956
                     /locus_tag="P5516_09020"
                     /inference="COORDINATES: protein motif:HMM:NF020419.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HRT92607.1"
                     /translation="MSSSLVSGLQIVFEVVVNRLSAAGVECLLVGGFAVNHYGYSRST
                     LDIDFMVVSDDRDAVLDAMQQAGFTSYSIHANVTFFQRPGETLRVDFLNTDSETMAKL
                     VSRAVRAEFFGVSVRLPALRDLLAMKFFALSQALGQRTEKDIPDIAWLTLINGLDIEA
                     DLHPLALRFADEEVFRRVCDKIEEIRA"
     gene            953..1225
                     /locus_tag="P5516_09025"
     CDS             953..1225
                     /locus_tag="P5516_09025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92608.1"
                     /translation="MSGNATLPWFKMPPRVNTADYLAFVSELLARADPAKAARQKTLE
                     ERIATPFRLMDDAPQPASRLNQHLTQAQPATQFNLTQRHKGHKGFF"
BASE COUNT          401 a          518 c          489 g          457 t
ORIGIN      
        1 ccaaccccca aacctcagcg tctccgcgtc ttagcgtctc tgcgttaaaa ctctctctgc
       61 cgcacgccaa cagcttaacg ccgaacgcag aacgccgaac gcagaacgcc gaacgccgaa
      121 gtgccgggag gggcaagcgt ctgcttgccc tggcaatgaa agccgggcgg tctcgccgag
      181 accgccgccg actggacgag cagacgctcg tccctcccgg attgcagcgg tcagagatca
      241 gcggtcagcg ccgcaaaacg attcacgatt gagcccgccg cccgcagagt gggaacaaac
      301 gatggggtaa ccttatccgc agatacgctg attacacaga ttgagtcatt ttctgcagaa
      361 atctgcgtaa tcggcggaca aaaatgtaaa gtatgagttc ctctctcgtc agtggcctgc
      421 aaattgtttt tgaggtggta gttaatagac tgtctgccgc aggggtggag tgtttgctgg
      481 tgggcggttt tgcggtcaat cactacggct attcgcgcag cacgttggat atcgacttca
      541 tggttgtttc ggacgaccgc gacgcggtgc tggacgcgat gcagcaggcg gggttcacga
      601 gttattcaat acatgcgaat gtaacctttt ttcagcgtcc gggggaaacg ttgcgcgttg
      661 attttctgaa cacggactct gaaacgatgg cgaagctggt gtcccgtgcg gttcgggcgg
      721 aattttttgg ggtcagtgtc cggctgcctg cattacgaga cttgctggcc atgaaattct
      781 tcgcgctgtc tcaggcgctg ggacaacgca cggaaaaaga cattcctgac atcgcctggc
      841 tgactttgat taacggattg gatatagagg cagaccttca tccgttggcg ctgcgcttcg
      901 cggatgaaga ggttttccgg cgagtctgtg ataagattga ggagattcgg gcatgagcgg
      961 aaacgcgacg cttccttggt tcaagatgcc gccccgagtg aataccgcag attatctggc
     1021 gttcgtgtcg gagctgctgg cccgggctga tccggcgaag gccgccaggc aaaaaaccct
     1081 tgaggagcgg attgccacac ccttccgcct gatggatgat gccccacagc ccgcaagccg
     1141 gttgaaccag cacctaacgc aggcgcagcc cgcaacccaa ttcaatctca cacaaaggca
     1201 caaaggccac aaaggcttct tttaaaacgg catccacctt cgtgcccttc gtggcttcgt
     1261 gtgagaaaca acttgttttt tattgctggt gttggttcgt aaggcactaa ggtgcgattg
     1321 aacccctgaa cctttgaacc agcgtcgcag gcgcgattgt cagcggtcag cgccgcaaaa
     1381 acgattcact cttcaatctt cactcttcac tcttcgttat tcactttcct tcctcctttc
     1441 tccttcctcc ttattccctc ctccctcctc ctttctcctt attacttatt ccctattact
     1501 tcttacttct tccctcttcc tcatgtctcc tgaaatcgcc aaatttacgc ccgcactttt
     1561 ttgggatgtc gatcgcgaaa gtctcgatat cgaaaaaagc agggctctca ttgttcagcg
     1621 tgtactcgag cgtggtcatg attcggactg ggaactgttg aaagcgtgtt atacggtcca
     1681 agggatcatt gatacggcca aaaaactgcg ttcgcttgag ccgaccgctt tagcttttgc
     1741 ctcgtgtata gggaatgcca agaaggaaga tttccgatgc tgcacttgga aacagtccac
     1801 cccgacacat tgggtttact gaagcgcctt caggccttgc ccgtcctttc cgaaacacgg
     1861 ctggt
//
LOCUS       DAOTLW010000116         1851 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_50632_length_1851_cov_12.513363, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000116 DAOTLW010000000
VERSION     DAOTLW010000116.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1851)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1851
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_50632_length_1851_cov_12.513363"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(126..446)
                     /locus_tag="P5516_09030"
     CDS             complement(126..446)
                     /locus_tag="P5516_09030"
                     /inference="COORDINATES: protein motif:HMM:NF023376.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3467 domain-containing protein"
                     /protein_id="HRT92609.1"
                     /translation="MMADQKQIKLNLPPDLQPIFSNMIRVGHTPAEFVLDFFKLLPSM
                     ETVNVDTRIVLTPLGAKMLLHVLSENIQHYEASFGEIRMPSPPHTLADDLFNLGGQSP
                     TDGK"
     gene            complement(453..836)
                     /locus_tag="P5516_09035"
     CDS             complement(453..836)
                     /locus_tag="P5516_09035"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HRT92610.1"
                     /translation="MYQKLILIGNLGNDPEMRYMPDGKPVTSFRVATNRRFKDKDETT
                     WFKITVWGAQAESCNQYLHKGSKVLVEGRLNPDTNGSPKVYQRRDGTWGANYEVSAQV
                     VRFLTPRGEGVGVEEEEAPEDDIPF"
     misc_binding    1072..1364
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            1503..>1851
                     /locus_tag="P5516_09040"
     CDS             1503..>1851
                     /locus_tag="P5516_09040"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="catalyzes the formation of L-tryptophan from indole
                     and L-serine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal-phosphate dependent enzyme"
                     /protein_id="HRT92611.1"
                     /translation="MTEETRTRFDLDQNEIPRFWYNISADSPVPPAPVFDPATREPVT
                     PEALLKLFPMQLIEQEVTTDRFVPIPDEVREVYKLYRPTPLLRARRLERELQTPAHIY
                     YKYEGASPAGSHKL"
BASE COUNT          468 a          476 c          448 g          459 t
ORIGIN      
        1 cgcactgcta aggaagcatg ccgggtcagc tgccgagctt gggtcaaggc agcatcacgg
       61 cggacattct tgcgctcaaa atcctgccgg atctggtcgc atatctggtc gagttcatcc
      121 atgagctatt ttccatccgt tggcgattga ccgcccaggt tgaaaaggtc atcagccagt
      181 gtatgcggcg gtgagggcat ccgaatctcg ccaaaagaag cctcatagtg ctggatattt
      241 tcactcaaca catggagcag cattttggcg cccaatgggg tgagcacaat gcgggtatcg
      301 acattgaccg tttccatact gggcagcaat ttaaagaagt ccaacacaaa ttccgccggg
      361 gtatgcccca cgcgaatcat attggaaaag ataggctgca aatccggcgg caaattcagc
      421 ttaatttgtt tttggtcagc catcatgccg ccttagaagg gaatatcgtc ttctggcgct
      481 tcttcctcct ccacgccaac cccttctcca cgcggtgtta ggaagcggac gacctgtgcg
      541 ctaacttcgt agttggcacc ccacgtgcca tcccgcctct ggtaaacttt gggcgagcca
      601 ttggtatccg ggttgaggcg cccttccacc aaaaccttag agcctttatg gaggtattgg
      661 ttgcagcttt ctgcttgggc accccaaacg gtgattttga accaggtagt ttcatcttta
      721 tctttaaagc gccggttggt agcaacgcgg aaagaggtaa caggttttcc atcgggcatg
      781 tagcgcatct ctgggtcatt accaagatta ccgatcagaa ttaatttttg atacatggga
      841 ttgttccttt cattttgagt gtaaatgcgt ccatatttgt tcgtcagcag catgtaatct
      901 gacttgaata aagcgatacg acaattttac tgcataatgt gattatggct ggatgaaaat
      961 cgaaatccaa tccctgccat tagggtgttg aaaaatgata agttttcaat actttcgcct
     1021 aaaaaccttg acaaatcacg gaccttgcct ataattttat ctaattgaac agtaaagacg
     1081 ttgaagagga cgagtaactg ccgaaccggt ggcacagaga gcagggctaa gctgaaaacc
     1141 tgcccgcacg accgcagcga atggacctca gagttgccag gcgaacacac ccgtcagtag
     1201 ccccggccgg agatgccacc gttatctagg taaagggtat cagctttggg ctcgtacccg
     1261 caatgagtga ggctggctat ttccgtgtgt tcttcaccgg aaattttttg taaaatcagc
     1321 ctaagttggg tggcaccgcg gatttcaaca catccgtccc tcagtgggat ggatgtgttt
     1381 tttgttaatg ccaaaggagg ctttattgaa gatgaaaatc aaaacctata agagcgtttt
     1441 gggcagcaaa gcctgccttt ctaaataaaa acaaaagtta atacgttcaa aaggagatta
     1501 tcatgacaga agaaacccgt acccgctttg acctggatca aaatgagatc ccgcgctttt
     1561 ggtacaacat tagcgccgat agccctgtgc caccggcgcc tgtttttgac cccgccaccc
     1621 gcgagcctgt caccccggaa gcgctgctta agctctttcc tatgcagctc atcgagcagg
     1681 aagttaccac cgaccgcttc gtacctatcc ccgacgaggt acgcgaagtt tataagcttt
     1741 accgccccac gcccctcctg cgtgcccgac ggctggagcg cgaactgcaa acacctgcgc
     1801 atatctacta taaatatgaa ggcgcttccc cggcaggcag ccataagctc a
//
LOCUS       DAOTLW010000117         1828 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_51533_length_1828_cov_19.882685, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000117 DAOTLW010000000
VERSION     DAOTLW010000117.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1828)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1828
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_51533_length_1828_cov_19.882685"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..517)
                     /locus_tag="P5516_09045"
     CDS             complement(<1..517)
                     /locus_tag="P5516_09045"
                     /inference="COORDINATES: protein motif:HMM:NF039542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5806 family protein"
                     /protein_id="HRT92612.1"
                     /translation="MDDPENLKDPNRYQKFKKVDGATYKRVNQFLRKHTYITAREWAI
                     ARLCADFKTSSGSEMTFIGENLPKLCPFMVDTYTPQAVNQARSSFKKKVKKAGATFFY
                     GAMCGFFTAEELDEILFEASEVARFLLEVEGTSLNLDDEIDIEDRITEVMRGVAEAAS
                     VILKTRPGQEGE"
     gene            1131..1418
                     /locus_tag="P5516_09050"
     CDS             1131..1418
                     /locus_tag="P5516_09050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92613.1"
                     /translation="MGGGLARYIWFLAFWGCFGYTCFSALEDAGFQVLEPLMPAKSFA
                     GGELTRYCVPQAQGCKTWRDYPSSMRRGHFPVIVDPGHLHIALLSQDRALC"
BASE COUNT          359 a          478 c          512 g          479 t
ORIGIN      
        1 tctcgccttc ctggccgggc ctggtcttca ggatgaccga ggccgcctca gccacacccc
       61 gcatcacttc agtgatcctg tcctctatat cgatctcatc atcgaggttc aggcttgtcc
      121 cctcaacctc aagcaggaac cgggcaacct cgcttgcctc aaaaaggatc tcatcgagtt
      181 cttctgcggt gaagaaaccg cacatggcgc catagaagaa tgtggcgccg gctttcttca
      241 cctttttctt gaacgaactc cgtgcctggt tgaccgcttg cggggtgtat gtgtctacca
      301 tgaacgggca gagtttgggg aggttctcgc cgataaatgt catctccgag ccgctggagg
      361 tcttgaaatc cgcgcagagg cgcgcgatag cccactcgcg ggcggtgatg taggtgtgct
      421 tgcgcaggaa ctgattgacc cgcttatagg tggcaccgtc taccttcttg aatttctggt
      481 agcggttagg gtccttgagg ttttcagggt catccatttt actatcctct tgttggtttt
      541 gctatttcaa taaggctggt ctggacagga gaggggagtt ttattcttcc gccagttctt
      601 ccagacaaat caatggggga gagatcggga ctttccgcgt cggggtctgg ctgtggttcc
      661 ataagagacc tggttttcaa ccaggatatg gtttatgacg ggtgaatctc acattatgga
      721 gtccatcaaa ccgggtcgct cgcggtttga tacccataaa taaccatcgc gcgaaacgtc
      781 tttttgcggt tgatcctacc gcccggccgc tcgagagggg gcgggcggtg tggggtgata
      841 tctccggtgg ccgtgccagg gagcgaaacg gaaattttcg gaaggattcg ctgaaaatct
      901 gtgtgagtga agtcaacttc tggcactgta tcgatctgga atcgccagta aggaggtttc
      961 ggtgagagcc tgagaaataa ttctcttcca aaaaatcccg tctgcacccc ccaacctcca
     1021 cctcgcgctc tttttcccgc accgcccctg gccgaccatc atcacccgga ccaatgtata
     1081 gcaacatatg gtttatgacc ctcctcccaa ggcactgttc tgcagggttg atggggggcg
     1141 gcctggcgcg ttatatttgg tttctggcgt tctgggggtg ttttggttat acatgctttt
     1201 cagcgctgga agacgccggg tttcaggtgc tcgaaccatt gatgccggca aaatcctttg
     1261 caggggggga actgacccgt tactgtgttc cacaggccca gggctgcaaa acctggcggg
     1321 attatccgtc atcaatgaga agagggcatt ttcccgtgat cgtcgatccc ggacaccttc
     1381 atatcgcgct tttaagccag gatcgcgccc tttgttgaag tgtggattgc caggggaagc
     1441 gatctggcgt ccatgccagt ctctggaggt cgtttatata caagggtggt ctgcggccgg
     1501 ggagggtggc atgttcacat cactttttac tttcacgccg cgcggtaagg ggttcatata
     1561 ctctcaagag aatgtattca acagggttct gcccccctcc ttccaccagg aggtggcgga
     1621 cccgcatcat acctcctaac tccgatccaa tcagcatatc tcccaaacct tttttggcac
     1681 cccggatagt ccggggcgtt ctggctgtgg gtcggggttt gcatcactgt tgagggagca
     1741 tcttaagatc gtgtatctgg tcgccccccc gctgggtatg gataagttgc ggggaatagg
     1801 catttaccgc aattttgata tgctttga
//
LOCUS       DAOTLW010000118         1815 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_52093_length_1815_cov_10.807955, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000118 DAOTLW010000000
VERSION     DAOTLW010000118.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52093_length_1815_cov_10.807955"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..810
                     /locus_tag="P5516_09055"
     CDS             <1..810
                     /locus_tag="P5516_09055"
                     /inference="COORDINATES: protein motif:HMM:NF020926.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1998 domain-containing protein"
                     /protein_id="HRT92614.1"
                     /translation="ERNTTIEAFNPRQQEVFSRHSRTFGDLNLRQEVSGYRKVRWQTG
                     ETIGHGEVHLPPSFLETKGCWLALEPELVDTLRAENLWTAGANNYGSGWAALKRQILM
                     RDGYRCRACGTSGDESTLHVHHIQPFKTFESLELANRPSNLVTLCPACHRLAEQNVRL
                     RSGLAGAAYALGNLAPLMVMCDREDLGMLSEPRSVLAHGQPAILVYDSIPGGLGLSEH
                     LYEQHALWISRAIEMIAECPCHDGCPACVGPIGEEGHGGKKEALALLEGLV"
     gene            812..>1815
                     /locus_tag="P5516_09060"
     CDS             812..>1815
                     /locus_tag="P5516_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024873.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HRT92615.1"
                     /translation="MDFESLSEKLRRMGVQIGVQKPLETPRQARKPIETVVPGREYQT
                     NFGSLFSLGHSYPQDYLHGRQPVLPQHPIYGLARWSRVPELEQKNLDQFIFLDTETTG
                     LSGGTGTMAFMVGVARFRGESLAMEQFFLRSPAEEAALLAGLEEFCDGMAAVVTYNGK
                     SFDIPILNTRYILQGFTSPFEDLPHLDLLHLTRRIWRARLEQCNLGNIEQQILQLQRD
                     GDEVPGYLVPEYYAQYLRDGNAEPLRGIFYHNEQDVLSLAALFALFADMLEAPTAWET
                     SSSQDLTALGRLLEHMGEVDGAVSLYQKGAQTAESARTKLEPLLAQAKLHKRHGEYNR
                     A"
BASE COUNT          416 a          481 c          507 g          411 t
ORIGIN      
        1 gagcgcaaca caactatcga agctttcaac ccgaggcagc aggaagtctt ctcccgccat
       61 agcagaacat ttggcgactt aaacctgcgg caagaagtaa gcggataccg caaagtgcgt
      121 tggcaaacag gcgaaaccat tgggcatggc gaagtgcacc taccgcccag ctttttggaa
      181 accaaagggt gctggctggc gctagagcct gaactggtgg ataccctgcg cgcagaaaac
      241 ctttggacag cgggcgcgaa caactacggg tccggctggg cggctctcaa gcgtcagatt
      301 ttgatgcgcg atggctatcg ctgccgcgct tgcggtacca gcggcgatga aagcacattg
      361 catgtgcatc atatccaacc attcaagact ttcgaaagtt tggagttggc taaccgaccc
      421 tcaaacctgg tgacgctctg cccggcttgc caccgcttag cggagcaaaa tgtgcgcttg
      481 cgcagcggct tagccggcgc tgcttatgcc cttggcaacc tggcaccact gatggtgatg
      541 tgcgaccggg aggatctggg catgctttcg gagccgcgtt cagtgctcgc tcatggtcag
      601 cccgctattc ttgtttatga cagcattcca ggtggattgg gactttcgga acacctttac
      661 gagcagcatg cgctttggat cagccgagcc attgaaatga tcgcggagtg cccgtgccat
      721 gatggctgcc ctgcatgtgt tggcccaatt ggtgaggaag ggcacggtgg taaaaaagaa
      781 gccttagccc tgctggaagg gttggtttga gatggatttc gagtcgctca gcgagaagct
      841 gcgccggatg ggtgtacaaa ttggcgtaca aaagcccttg gagactccca ggcaggcacg
      901 caagccgatc gaaacggttg tccctgggcg tgaataccag accaactttg gcagcctatt
      961 ttccttgggg cattcctacc cgcaagatta tctgcatggc aggcagcccg ttctccccca
     1021 gcatccgatt tacggattag cccgctggtc gcgcgtccca gaattagaac aaaaaaacct
     1081 cgatcaattt atctttttgg atactgaaac taccgggctt tccggaggta ccggcacgat
     1141 ggcgtttatg gtcggggtag cgcgtttccg cggagaatct ttagctatgg agcaattctt
     1201 tctgcgcagc cctgctgagg aagcggcttt actggctggc ttggaagaat tttgcgacgg
     1261 tatggcagcg gttgtgacct acaacggaaa atcattcgat attcccattc tcaatacgcg
     1321 ctacatcctg cagggtttta ccagcccttt tgaggatcta ccgcatttgg acctgctgca
     1381 cctcacccgc cgcatctggc gggcgcgctt ggagcaatgt aacctcggta acatcgagca
     1441 gcaaatcctg cagctccagc gggatggaga tgaagtccca ggttatttgg taccagaata
     1501 ctatgcgcag tacttgcgtg atggaaatgc ggagcctttg cgcggaattt tctaccataa
     1561 cgaacaagat gttctaagcc tggcagcttt gtttgcctta ttcgcggata tgctcgaagc
     1621 acccacagcc tgggaaactt ccagttctca agacctaaca gctttaggtc gtttgctcga
     1681 acacatgggc gaagtagacg gcgctgttag tctgtatcag aagggcgcac aaacagccga
     1741 gtccgctcgt actaagttag agcccctctt agcacaggct aagctgcata aacgccatgg
     1801 ggaatacaac cgcgc
//
LOCUS       DAOTLW010000119         1796 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_52870_length_1796_cov_6.443423, whole genome shotgun sequence.
ACCESSION   DAOTLW010000119 DAOTLW010000000
VERSION     DAOTLW010000119.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1796)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1796
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_52870_length_1796_cov_6.443423"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1634
                     /locus_tag="P5516_09065"
     CDS             <1..1634
                     /locus_tag="P5516_09065"
                     /inference="COORDINATES: protein motif:HMM:NF012738.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HRT92616.1"
                     /translation="LIFFYQPLFAILRLAFSSTFDGGWQGASAAQVLKPLGFTLWQAV
                     LSTLLTLLVGLPAAYLFSHYEFKGREGLHLLSLLPFILPTVVVAACFNTLLGPRGWLN
                     LGLMALLNLSEPPIRMLQSLPAILLAHVFYNTAIIIRLVGSAWSRLDQKLVHAAQMLG
                     ASPWQAFREVTLPLLLPAITGASLLVFLFDFTSFGVVLMLGGPQFATLEVEIYTQALY
                     MLNLRLAAVLSLVQLACTLLVAWLQSRASQPRYGKAAVSAERANLRPARSAGEKAFIA
                     VMVILLVMLILTPLLSLITRSFVTLEAARGERGQIQTGFTLRYYQELFQNRTGSLFYV
                     PPIIAVRNSLVYALLTVVIATSLGLLGAYALNQPWKANRILEPFFMLPLGASAVTLGL
                     GFLVAFRQPAWSSMPFPLLIPIAHALVALPMVVRTLLPALRSIPSSLRQAAGTLGADE
                     PRVFREVDLPLLLRPLLVSMVFAFTISLGEFGASSFLSSAQTPTIPVAIYRYISQPGA
                     LNYGQALAMSTLLLVVCGMGIFLIEKIRLPGEELF"
     gene            1634..>1796
                     /locus_tag="P5516_09070"
     CDS             1634..>1796
                     /locus_tag="P5516_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HRT92617.1"
                     /translation="MLEVREIHKTYEGLPLLAGVSFEVRSGKIVCLLGESGSGKSTLL
                     RIIAGLEPSD"
BASE COUNT          308 a          524 c          510 g          454 t
ORIGIN      
        1 tgctgatttt cttttaccag ccgctttttg ccatcctgcg cctggcgttt tcgtccactt
       61 ttgacggggg ctggcaaggc gcaagcgcgg ctcaggtgct caagccattg ggcttcaccc
      121 tctggcaggc agtgctctcc acgctgctaa cgcttttggt gggcttgccc gcggcttatc
      181 tcttttcgca ttatgaattt aagggacgcg aagggttaca cttgctcagc ctgctgccgt
      241 tcattctgcc gacggtggtg gtagcagcct gcttcaacac gctgctaggc ccacgcggct
      301 ggctgaactt agggctgatg gcacttttga acctttccga gccgccaatc cgcatgctgc
      361 agagtttgcc ggcgatcttg ctcgcgcatg tgttttacaa taccgcgatc atcatccggc
      421 tggtaggctc agcctggagc cggctggacc aaaagttagt gcacgccgcg cagatgctgg
      481 gcgctagccc ctggcaagct ttccgtgagg tcaccctgcc gctgctcttg ccagccataa
      541 ccggcgccag cttgcttgtg tttctgtttg atttcacgag ttttggtgtg gtgctcatgc
      601 tgggcgggcc gcaatttgcc acgctggaag tggaaatcta cacccaggct ctatatatgc
      661 tcaacctgag gctcgcagca gtgctttcgc tcgtgcaatt agcttgcact ttactcgtag
      721 cttggctgca gagccgtgca agccagccgc gctacggtaa agccgccgtg agcgccgagc
      781 gcgccaacct gcgccctgca cggagtgcgg gggaaaaagc tttcatagcg gtgatggtaa
      841 ttctcttggt gatgctcatc ctcacgccgc tgctcagcct catcacgcgc agctttgtga
      901 ctctggaggc agcccgaggc gagcgcgggc agatccaaac tggcttcacc ttgcgctatt
      961 accaggagct ttttcaaaac cgcaccggat cgctttttta tgtgccgccc atcatagcag
     1021 tacgcaactc gctggtctat gcactgctca cggtcgtgat cgccacaagc ttggggctgc
     1081 tcggggcgta tgcgctcaac cagccctgga aagctaaccg gattctcgaa cccttcttta
     1141 tgttgccttt gggcgccagc gccgttacgc ttggcttagg ttttttagtc gctttccggc
     1201 agccggcttg gagtagtatg ccctttccgc tgctcatccc gattgcgcat gcgctagttg
     1261 cgctgcccat ggtggtgcgc acgctgctgc cggcgctgcg tagcattccc agctctttgc
     1321 gccaggcagc tggcacactc ggggcggatg agccgcgcgt attccgcgaa gtggatctgc
     1381 ccctactcct gcggccgctg ctggtgagca tggtcttcgc cttcaccatc tcgctaggag
     1441 aatttggggc ttccagcttt ctgagcagcg cgcaaacacc caccatccca gtggcaattt
     1501 accgttacat ttcgcagccc ggcgcgctca actacgggca agcactggcg atgtctacct
     1561 tgttactcgt ggtgtgcggc atgggcatct ttttgattga aaaaatccgc ttacctggag
     1621 aggaactttt ctaatgctgg aagtgcgcga aatccataaa acttatgaag ggctgccgct
     1681 cttggctggt gtttcttttg aggtgcgcag cggaaaaatc gtctgtctgc tgggcgaatc
     1741 aggcagcggt aagagcacat tgctgcgcat cattgctgga ttggagccga gtgatt
//
LOCUS       DAOTLW010000120         1750 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_54977_length_1750_cov_6.483186, whole genome shotgun sequence.
ACCESSION   DAOTLW010000120 DAOTLW010000000
VERSION     DAOTLW010000120.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_54977_length_1750_cov_6.483186"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="P5516_09075"
     CDS             <1..1158
                     /locus_tag="P5516_09075"
                     /EC_number="2.8.4.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MiaB/RimO family radical SAM
                     methylthiotransferase"
                     /protein_id="HRT92618.1"
                     /translation="DLVVVNTCSVTAAAASDSRQKVRQAQHAGASNILVTGCLATLEP
                     SEMARLPGVKRVVSNQAKPQIAGLVMGQLADFDLEPLARQPLPGARKRTRAFIKVQDG
                     CNNHCTFCVTRIARGEARSIPRDEVLREVNHALHGGSREVVLTGVHLGWWGRDLRHEE
                     TLPDLVGYLLEKTTIERLRLSSTEPWDLDDRFFDLWQDPRMCRHLHLPLQSGSAEVLK
                     RMARRTSLVEYAQLLAHARERIPGLAVTTDIIVGFPGETEDEFSESLEFVRSMNFAGG
                     HVFRYSARPGTAAARLSGRVHGTIARERGRLMRLALEKSARHYADGQVGCELKTLWEA
                     AEPQDGGWLVRGLTDNYLPVTAQAGSDRSNEIDWVRIDGYKKGSLCATITG"
     gene            1174..>1750
                     /locus_tag="P5516_09080"
     CDS             1174..>1750
                     /locus_tag="P5516_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRT92619.1"
                     /translation="MNDIAKHHWWQKAVFYQIYPRSFADGNDDGIGDFAGMISRLDYL
                     QQLGVDAVWLSPHYPSPYVDCGYDISDYQGVAPEYGKMHDFRQFLDGLHQLGMKLVLD
                     LVLNHTSDQHPWFIESRSNRHNPKRDWYIWKDGKEGQPPTNWYSTFGGSAWEYDKLTS
                     AYYYHYFFKEQPDLNWRNPAVKEAMFNVARFW"
BASE COUNT          405 a          441 c          523 g          381 t
ORIGIN      
        1 gacctggttg tggtaaacac atgctcggtc actgcggccg cggcttctga ttcccgccaa
       61 aaagtaagac aggcacagca cgccggcgcc tcgaacatcc tggtaaccgg ctgcctggcc
      121 accttagaac cgtctgagat ggcaaggttg cccggcgtca agcgggtggt ttccaaccag
      181 gctaagccgc aaatagcagg gctggttatg gggcaattgg cggattttga ccttgaaccg
      241 ctggccaggc agcctttgcc tggagcgcgc aaacgcaccc gggctttcat aaaagtgcag
      301 gatggatgta ataaccattg cactttttgc gtgacgcgca tcgctcgcgg tgaagcgcgc
      361 agtataccca gagatgaggt attacgcgag gtcaaccacg ccctccatgg aggtagccgg
      421 gaggtggtat tgacgggtgt gcacctgggc tggtggggcc gagatttacg ccatgaagaa
      481 accttacctg accttgtggg ctacctgttg gaaaaaacga cgatagagcg gctacggttg
      541 tcttcaactg aaccttggga cctggatgac cgcttttttg atctctggca agacccgcgc
      601 atgtgccgcc acctgcatct acccttgcag tcaggttcag cggaagtgct caaacgcatg
      661 gcaaggcgca cttcactggt tgaatatgct caacttctcg cgcatgccag ggaacgcatt
      721 ccggggctgg cagtgacaac ggatatcatt gtcgggtttc cgggtgagac ggaagatgag
      781 ttcagcgaga gcctggaatt tgtcaggtcg atgaactttg ctggcgggca tgttttcaga
      841 tactccgcca gaccgggcac cgccgcggcc aggctctccg gcagggttca cggcaccatc
      901 gctagagaaa gaggtcggct gatgcggctg gcgctggaaa agtcagcgcg ccattacgca
      961 gatggacagg tcggttgcga gttgaagacg ttgtgggaag cagccgagcc gcaagatgga
     1021 ggttggttgg tgcgtggatt gacggataat taccttccgg ttaccgcgca ggcggggtca
     1081 gaccgaagta acgagataga ctgggtgaga atagatggat ataaaaaagg cagcctgtgt
     1141 gccacgatca ccgggtaaca gaagaggaac aggatgaacg acatcgctaa acatcactgg
     1201 tggcaaaagg cggtttttta ccagatttac ccgcgcagtt tcgcggatgg taatgatgat
     1261 ggcataggtg actttgccgg aatgatatcc aggctggatt acctgcagca actaggcgta
     1321 gatgccgtgt ggctttcacc tcattaccct tcgccgtatg tggattgtgg ctacgatatc
     1381 tctgattacc agggagtggc gccggaatac ggcaaaatgc atgatttccg ccagttcctg
     1441 gatgggctgc accaactcgg catgaaactg gtgcttgacc tggtgctgaa ccatacttct
     1501 gatcagcacc cctggtttat cgaatcacgc tccaaccggc acaaccccaa acgcgactgg
     1561 tatatctgga aggacgggaa agagggccaa ccgcctacga actggtactc aacctttggc
     1621 ggttctgcct gggagtatga taagctgacc tctgcgtatt attaccatta ctttttcaaa
     1681 gaacagccag acctgaactg gcgtaacccg gctgttaaag aagccatgtt caacgtggcg
     1741 cgtttttggc
//
LOCUS       DAOTLW010000121         1710 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_56839_length_1710_cov_7.926284, whole genome shotgun sequence.
ACCESSION   DAOTLW010000121 DAOTLW010000000
VERSION     DAOTLW010000121.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1710)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1710
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_56839_length_1710_cov_7.926284"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="P5516_09085"
     CDS             <1..296
                     /locus_tag="P5516_09085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92620.1"
                     /translation="AILVWVIRILIIGSLTSSLEQSSSARKAVRRVDGLNQPAGSRPA
                     VGFSRPQSSTPSARSVNPRASVTSMVRSSNACVGQPIQRNEFMAAEEQSAYKV"
     gene            416..1570
                     /locus_tag="P5516_09090"
     CDS             416..1570
                     /locus_tag="P5516_09090"
                     /inference="COORDINATES: protein motif:HMM:NF013755.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HRDC domain-containing protein"
                     /protein_id="HRT92621.1"
                     /translation="MPTRPSASPIWIATQDDLLKMTDAITAEPLLAIDTESNSLYAYR
                     ERVCLVQVSTPQTDYLVDPLALTDLSAFGSLFADSRRQKIFHAAEYDILCLKRDYNFT
                     FNNIFDTMIAARILSYPQVGLGNLLSAWFGIELEKKYQRADWGKRPLPQAMLDYARMD
                     THYLFDLRTRFERELKEKRLWELALEDFSLVCQAVPAPADNNGGTCWKVIGGKHLSAK
                     ETAVLQALCAYRDQQAQRMGLPHFKVLSNTLLLELSHAMPRTLDELKSVSGMNDVLLK
                     RHGTGLLRAIEEGKRAKPPARPPRQRPEKDVLNRLKRLHEWRKTLAGSLKTESDVVLP
                     RVFMEHIASENPQGLADLKELMADIPWRYRQYGKAILSVIHRQEEYEDSL"
BASE COUNT          407 a          473 c          443 g          387 t
ORIGIN      
        1 tggccatcct ggtttgggtc attcgcatcc tcatcatcgg gtcgctcacc tcttcacttg
       61 aacaatcgtc atcggcgcgc aaagcggttc gccgcgtcga tgggttaaac cagccggctg
      121 gcagccgccc tgccgtcggg ttcagccgtc cgcaatcctc aaccccctcc gcccgttcgg
      181 taaacccgcg tgccagcgtg acctccatgg tcaggtcaag taacgcgtgt gtcgggcaac
      241 ccatccagcg caacgaattc atggcagcag aagaacaaag cgcttataag gtttaacctt
      301 ccctcgcccc ccaaaagccc ggcactgatg ctgggctttt tttgaccaac cagttgtgaa
      361 gaatcatctt gtttttaaaa accaatcaag ggaatcgcgg gtttataatt cgtctatgcc
      421 tacccgtccc agcgcaagcc caatttggat cgccacacag gatgacctgc tcaagatgac
      481 agacgccatc actgctgaac cgttgctggc catcgatacc gaatccaatt cgctctatgc
      541 ctaccgcgag cgggtttgcc tggttcaggt ttcaacaccg caaacagatt acctggtgga
      601 tccgctggcg ctgactgatc tttccgcgtt tggaagcctg ttcgctgatt ctcgccggca
      661 gaagatcttc cacgctgcgg aatacgacat cctgtgtctg aagcgcgatt acaacttcac
      721 cttcaataat attttcgata cgatgatcgc cgcgcgcatc ctttcctacc ctcaggtcgg
      781 gctgggcaat ctgctttctg cctggtttgg tattgaactg gaaaagaaat atcaacgcgc
      841 cgattgggga aagcgacccc tgccccaggc catgctggat tacgcccgta tggataccca
      901 ctatctcttt gaccttcgga cgaggtttga aagagaattg aaagaaaagc ggttgtggga
      961 actggctttg gaggattttt cgctggtgtg ccaggcggtt ccagctcctg cagacaataa
     1021 tgggggtacc tgctggaaag tcataggagg gaagcatctg agcgcgaaag aaacggccgt
     1081 gttgcaggcg ctatgcgctt accgcgatca gcaggcgcag cgcatgggtt tgccgcattt
     1141 taaagtgctt tcaaataccc tgttattgga gctcagccat gccatgccgc gcacgctgga
     1201 tgaactcaaa tctgtcagtg gaatgaacga tgtcctactc aagcgccacg gaaccggcct
     1261 gctgcgcgcg attgaagaag gtaaacgtgc caaaccgcca gcacgccctc cgcgccagcg
     1321 tccagaaaaa gacgtgctga acaggctaaa aaggttgcac gagtggcgaa aaacgctggc
     1381 tggcagtcta aaaacagagt cagatgtggt gctaccgcgc gtgttcatgg aacacatcgc
     1441 ttctgaaaac ccgcaagggc ttgccgacct caaagaactc atggctgata ttccgtggcg
     1501 atatcgccag tacggcaaag ccattttgtc ggttattcac cgtcaggagg aatatgaaga
     1561 ttcactttaa cggggcggcg cgaacagtga caggttcgca gcatcttatc gaagtcaaca
     1621 atcataagct gttattggat tgcggccttt ttcagggcaa acgggctgaa gcctatgaaa
     1681 aaaaccgcca tttcagtttc aaccccgatg
//
LOCUS       DAOTLW010000122         1687 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_57906_length_1687_cov_7.615809, whole genome shotgun sequence.
ACCESSION   DAOTLW010000122 DAOTLW010000000
VERSION     DAOTLW010000122.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1687)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1687
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_57906_length_1687_cov_7.615809"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..759
                     /locus_tag="P5516_09095"
     CDS             <1..759
                     /locus_tag="P5516_09095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92622.1"
                     /translation="LKAAVVTRLAKEDEHVVNEIRAHGIDCYPVYSPSSTLMTLEYKT
                     SDVDKRSLYVKATAGAIRAADLSGLAARAIVVSPSLRGEVEADFFETQRKRQGVLLSA
                     DVQGFVRVLRGESLVYEPWPDMERVLRQLDVLKTDAVEAAYLTGEQDIETAARRFASL
                     GAKEILLSHSEGILVHAEGKFYHYLFHANSMSGRSGRGDTCVGSYVAKRLTLPPYEAG
                     KWSAATTSLKIERLGVFNRSVAEVEEFISRHYAD"
     gene            749..1639
                     /locus_tag="P5516_09100"
     CDS             749..1639
                     /locus_tag="P5516_09100"
                     /inference="COORDINATES: protein motif:HMM:NF024118.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT92623.1"
                     /translation="MLIDPLQRREKIQADLLKARSGYEVLWQQVTGDWRSAEKGCHGW
                     LTYSANYLFSFDGLKWGLDTYAMPARIQGMRSPEYRADLAPLSLMVLTHAHRDHLDTN
                     LIQALINSEVQWVIPQHVQEVLRGMGALPGKNVITPVPGQPIQKGALRLLPFESLHLR
                     GVYGVPETGYLVEYQNQRWLFPGDVRVYDNSRLPDFGRLDGVIAHLWLGKACAMDEEP
                     PFLDAFCQFFSKFDAEQLFITHLNEFGRDETELWCEEHFNLVKTNLQKRAPGLKVQKF
                     LMGDRMAFNPSAHRTEQKKP"
BASE COUNT          388 a          423 c          500 g          376 t
ORIGIN      
        1 ctgaaggctg cggtggtaac ccgcctggca aaagaagatg agcatgtggt caacgagata
       61 cgcgctcacg gtattgattg ttacccggtc tattctccat cttccaccct tatgacgctg
      121 gagtacaaga ccagcgatgt ggataaacgc agcctttatg taaaggcaac tgcgggggcg
      181 atccgcgcgg ctgatttgtc agggttggca gcccgcgcca tcgtggtgag ccccagcctg
      241 cgcggcgagg tggaagcgga cttttttgaa acacagcgga aacggcaggg ggtgttgcta
      301 tcagcggatg tgcaggggtt tgtgcgggtg ttgcgcggcg agtcgctggt gtacgaaccc
      361 tggccggata tggaacgcgt actgcgccag ctggatgttc tcaagaccga cgcggttgaa
      421 gcggcctacc tgaccggcga acaggatatc gaaactgcag cgcgccgttt cgcttcactg
      481 ggcgcgaaag aaatattgct ttctcacagc gaaggtattc tcgtgcacgc cgagggaaaa
      541 ttctatcatt acctctttca cgcaaactcg atgagcgggc gcagcggcag gggcgacacc
      601 tgcgtgggtt catacgtcgc gaaacggcta acgctacctc cgtatgaagc cggtaaatgg
      661 tcagccgcga ccaccagcct taagatcgag cggttgggag tgttcaaccg cagcgtggct
      721 gaggtagagg agtttatttc acgccattat gctgattgac ccgctgcagc gccgtgaaaa
      781 gatccaggcg gacctgctta aagccaggtc ggggtatgag gttttatggc agcaggtgac
      841 cggcgattgg cgcagcgcag aaaaaggctg ccatggctgg ttgacgtact cagccaacta
      901 ccttttttct ttcgacggtt taaagtgggg attggatacc tacgcaatgc ccgcgcgtat
      961 tcaggggatg cgctcgccag aataccgggc agatctggcg cccctttcgc tgatggtgct
     1021 gacgcacgct catcgcgacc atctggacac gaacctcatc caggcactca tcaattccga
     1081 ggtccagtgg gtcatccccc agcatgtaca ggaggtgctg cgcgggatgg gggcactgcc
     1141 tggaaaaaac gtcatcaccc ctgtgcccgg gcagcccatt caaaagggag cgctgcgttt
     1201 actgcccttt gaatcactcc acctgcgtgg cgtttatggt gtgcccgaga ccggctacct
     1261 ggtggaatac caaaaccagc gttggctgtt tccaggggat gtgcgtgttt acgataacag
     1321 caggttacct gatttcggcc ggctggatgg tgtgatcgcg cacctgtggt tggggaaagc
     1381 ctgcgcgatg gatgaagaac cccctttcct tgacgcgttt tgccaatttt tctcaaagtt
     1441 tgacgctgaa caattgttca tcacccattt gaatgaattt ggcagagatg aaactgagct
     1501 gtggtgtgaa gagcatttta acctggtaaa aaccaatttg caaaagcgcg cgccaggttt
     1561 gaaagttcaa aaatttttaa tgggagacag gatggcattt aacccatccg cccatagaac
     1621 ggagcagaaa aagccatgaa agagaccaaa gtgttagaca ataaggatat tttttgccat
     1681 gagctgc
//
LOCUS       DAOTLW010000123         1674 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_58538_length_1674_cov_16.408894, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000123 DAOTLW010000000
VERSION     DAOTLW010000123.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1674)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_58538_length_1674_cov_16.408894"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="P5516_09105"
     CDS             <1..852
                     /locus_tag="P5516_09105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-ubiquinone oxidoreductase-F iron-sulfur
                     binding region domain-containing protein"
                     /protein_id="HRT92624.1"
                     /translation="GEDIMGSGFAFDLHIHTGAGAYICGEETALIESIEGKRGEPRSR
                     PPYPTTEGLWGKPTVVNNVETLANIAPIILNGSDWFRSFGTATSPGTKVYTILGNLNV
                     TGLIEVPMGITLREIITLYGKGMKNGYFKMAQTGGSGGSIIPSSLQDTPMDFASFAKA
                     GVALGSGALLICNDEVCVVDLARVLLNFFRKECCGKCSPCRVGTEKAYQILSAICQGK
                     GKMEDLKTLEQISFQLNELSNCGLGQTAAVPLRDMLYRFRDEVEAHIRLNVCPAGACS
                     LSIPALV"
     gene            878..>1674
                     /locus_tag="P5516_09110"
     CDS             878..>1674
                     /locus_tag="P5516_09110"
                     /inference="COORDINATES: protein motif:HMM:NF024900.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HRT92625.1"
                     /translation="MITLTIDGIQVEVPEGTTVLEAAEAAGVKIPRLCYHPSLIPYGG
                     CRLCMVEVEGARILQPSCTLPVNNNMVVHTNTPKTQEARKFVLAMLFSERNHFCMYCQ
                     ETDGDCELQAAAYEQGLTHWPLTPDYKPYAVDASHPYIFQENNRCILCRRCVRVCGEK
                     VGNFTLGFEDRGASSILVADFGVPLGESSCVSCGACVQICPTGALMDRRSAYLGRETD
                     LAHTVSVCTDCSLGCERDVLTRDNHLVRIEGIWDSPMNEGLLCKQGR"
BASE COUNT          376 a          446 c          453 g          399 t
ORIGIN      
        1 ggtgaggaca ttatgggttc tggtttcgcc ttcgatctgc acattcacac aggcgcgggc
       61 gcttatattt gtggtgaaga aactgccctg atcgaatcta ttgaaggcaa acgcggtgag
      121 ccgcgctccc gcccaccgta ccccacgact gaaggattgt ggggtaagcc caccgtagtt
      181 aataacgtgg agaccctggc taatattgcc cccatcatcc tcaacggatc ggattggttc
      241 cgctcctttg gcactgccac cagcccaggt accaaggttt acaccatttt aggcaacctg
      301 aatgtcactg gattgatcga agtccctatg ggcatcacct tacgcgaaat catcaccctc
      361 tatgggaagg ggatgaagaa tggttatttc aagatggcgc aaactggtgg ttctggcggg
      421 tcaattatcc cttccagcct gcaggataca cccatggatt ttgcctcttt cgctaaggct
      481 ggtgtggctt tgggcagcgg agcgctgctg atttgcaacg acgaggtttg cgtcgtagac
      541 ctggcgcgag ttctgctcaa ctttttccgc aaggaatgct gtgggaaatg ctctccttgc
      601 cgggtaggca ctgagaaagc ttatcaaatc ctttctgcaa tctgccaagg caaaggcaaa
      661 atggaagatc tgaaaacctt agagcaaatc agcttccaac tgaacgaact ctctaactgc
      721 gggctcggtc agacggcggc tgttcccttg cgggatatgc tctaccgttt ccgcgacgaa
      781 gtggaagcgc atattcgctt aaatgtttgt ccagcaggcg cttgctcctt gagcattcct
      841 gccttggttt aacacacaat gagatggagc gagatttatg attacactca caattgacgg
      901 tattcaagta gaagttccag aaggcaccac cgtactggaa gcagctgaag cagccggggt
      961 caaaattccg cgcttgtgct atcaccccag tttgatcccc tacggtggct gccggctgtg
     1021 tatggtggag gtagaaggag cacgcatcct gcaacctagc tgcacgctgc cagtgaacaa
     1081 caacatggtc gtgcatacta atacgcccaa aacgcaggag gcacgcaagt ttgtgcttgc
     1141 aatgctcttt agcgagcgca atcatttctg catgtattgc caagaaaccg atggtgattg
     1201 cgagctgcaa gccgcagctt atgaacaggg actcacccat tggccgctca cgcctgacta
     1261 taagccttat gcggtggatg cctcacatcc gtatatcttc caagagaaca atcgctgcat
     1321 cctttgccgg cgctgtgtgc gcgtatgcgg ggagaaggta ggcaacttta ccttaggctt
     1381 tgaagaccgc ggcgcaagca gcatcttagt ggcggatttt ggcgtgccct tgggtgaaag
     1441 ctcctgcgtc tcctgtggcg cttgtgtgca gatttgcccc accggtgcgt tgatggaccg
     1501 ccgcagcgct taccttgggc gcgaaaccga ccttgcccat acggtttcag tctgcaccga
     1561 ctgcagcttg ggctgcgagc gcgatgtgct cacacgcgat aaccacctcg tacgcatcga
     1621 gggcatctgg gattctccca tgaacgaagg cttgctctgc aagcaagggc gcta
//
LOCUS       DAOTLW010000124         1644 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_60074_length_1644_cov_7.955947, whole genome shotgun sequence.
ACCESSION   DAOTLW010000124 DAOTLW010000000
VERSION     DAOTLW010000124.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1644)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1644
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60074_length_1644_cov_7.955947"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..995
                     /locus_tag="P5516_09115"
     CDS             <1..995
                     /locus_tag="P5516_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HRT92626.1"
                     /translation="AMTTETGAGQWGSALAMACAFFGLELEVYMVRVSYDQKPYRRFL
                     METYGAQVFASPSNRTTYGRKVLAEQPDTAGSLGIAISEAVEAAVTSGGKKKYGLGSV
                     LNHVLLHQTVIGEEALKQMDMAGEYPDVVIGCVGGGSNFSGIAFPFLRENLKHGQRTR
                     LLAVEPCATPSLTRGLYAFDYGDSACMTPIVKMHTLGHDFIPAAIHAGGLRYHGMAPA
                     LSALYDAGLVEAVAVHQKAVFESALLFTRTEGILPAPESAHALHAAINEALEARQTGE
                     KKVVLFNMSGNGYFDLTAYSRYLAGELEDYAYPQEDIHSIRQHLPAVKENAGLL"
     gene            1167..>1644
                     /locus_tag="P5516_09120"
     CDS             1167..>1644
                     /locus_tag="P5516_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92627.1"
                     /translation="MRKPLVYIPALAPRPAMPFERFLPLDPPGMVSRWLQKDLPAGSL
                     LLDPLGSSPQAILEAATSYRVLVACNNPVTAFQIRLQARASRQSEFNGVIRELGDLKK
                     GEERLEATIRNLYLTRCVSCGAEIQASGYIWNRGDSVPHTRLYTCPRCHDSGEHPVS"
BASE COUNT          363 a          455 c          448 g          378 t
ORIGIN      
        1 aagccatgac caccgagacc ggcgctggtc aatggggctc cgcattggcg atggcttgcg
       61 ccttttttgg tttggaatta gaagtgtaca tggtgcgtgt ctcgtacgat caaaagcctt
      121 atcggcgatt tctaatggaa acgtacggtg cccaggtttt cgccagccca agcaatcgta
      181 caacctacgg acgaaaagta ctggcagagc aaccagacac agccggatcg ctgggtatcg
      241 ccatctccga agcggttgaa gccgcggtca cctcaggcgg aaagaagaag tatggtcttg
      301 ggtctgtgtt gaaccacgtg ctgctgcatc agacggtaat cggcgaggag gcgctaaaac
      361 aaatggatat ggcgggagaa tacccggacg tggtcatcgg gtgcgtgggc ggcgggtcca
      421 acttctcagg tattgccttt cccttcttgc gtgaaaatct caagcacggg cagcgcaccc
      481 gcctgctggc agtggagccc tgtgccaccc cttctctcac ccgtggtctg tacgcgtttg
      541 attatggtga ttcggcctgt atgacgccaa tcgttaaaat gcacaccctg ggtcatgatt
      601 ttatccccgc agccattcac gccggtggtt tgcgttacca cggtatggcg cccgctttat
      661 ccgcgctcta cgacgccggg ctggttgagg cagtggcggt gcatcagaaa gcggtgttcg
      721 aatcggccct cttatttacc cgtacagaag gaatcctgcc tgcgcctgaa tccgcgcatg
      781 cgctgcacgc tgccatcaac gaagcgcttg aagccaggca aaccggagag aaaaaagtgg
      841 ttctattcaa catgtcgggt aacggttatt tcgacctcac cgcttatagc cgttacctgg
      901 cgggagagct ggaagattac gcttacccac aagaagacat ccattctatc cgtcagcatc
      961 ttccagccgt aaaagaaaat gccggtttgt tgtaaaaggc aacggctgtc acgcttttat
     1021 gaccagccct ggttggtcag cgctgttttt taccaccaag taggcttttt gaggtgagcc
     1081 atcttcctga ttaccggtcg ccggcagtgt tcagattgtt tgtacaaaaa acgcggattt
     1141 cggctatcat aaattcaggt gttgaaatga gaaaacccct ggtgtatatc cccgcattag
     1201 ctccgcggcc agccatgcct tttgagcgct tcctccccct ggacccgcct ggcatggtca
     1261 gccgttggct gcaaaaggat ctccccgctg gcagcctgct tttggaccca ttgggctctt
     1321 caccgcaagc catactggaa gctgccacca gttaccgggt gttagtggct tgcaacaacc
     1381 cggtgactgc ctttcaaatc cgcctgcagg ctcgcgcttc acgacaatca gaatttaacg
     1441 gcgttatcag agagttgggt gacctgaaaa aaggggaaga aaggttagaa gccaccatcc
     1501 gcaaccttta tctcacccgt tgtgtcagct gcggagccga aatccaggcc agcggttaca
     1561 tttggaaccg cggagattct gtgccgcaca cgcgcctgta cacctgcccg cgctgccatg
     1621 acagtggaga acacccggtt agcg
//
LOCUS       DAOTLW010000125         1641 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_60219_length_1641_cov_11.982976, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000125 DAOTLW010000000
VERSION     DAOTLW010000125.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1641)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1641
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60219_length_1641_cov_11.982976"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..804)
                     /locus_tag="P5516_09125"
     CDS             complement(<1..804)
                     /locus_tag="P5516_09125"
                     /inference="COORDINATES: protein motif:HMM:NF016736.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="baseplate J/gp47 family protein"
                     /protein_id="HRT92628.1"
                     /translation="MSFQKSFDELLNEILTDYRNQFPSADTSAGSLIFIKSACLASAL
                     WGLYKYQDWIAKQIFPDTADTQQLEHHAWVRGITRTYGESDAALLSRLLDDIRRPPAG
                     GNKFDYVKWAREVNNVARAWCYPIPAGALGHVEVVVLANSQTTGSEIPSLSARIGVVT
                     SAASGKLVDSGATFTGGHAVVAGDIVENPLRQTRTTVASVDSNTQISLNADIFPFAGE
                     PYIIHCQTGTNTSAEECKLIDSAGAFNDATYPVGVGDIVENVSDNKETTV"
     gene            complement(804..1181)
                     /locus_tag="P5516_09130"
     CDS             complement(804..1181)
                     /locus_tag="P5516_09130"
                     /inference="COORDINATES: protein motif:HMM:NF019055.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage GP46 family protein"
                     /protein_id="HRT92629.1"
                     /translation="MDFAITIEDQTGSGYMTFDKSQNGNLLNNIFLSLVVRRGSWFQD
                     PSFGSRLHLLARSKNTEQTAALAEEYCKEALQWLIDTGKATAVDVLSQRDRAQDPHRL
                     KLLITVTKANGDQVVFTTFVEVI"
     gene            complement(1185..>1641)
                     /locus_tag="P5516_09135"
     CDS             complement(1185..>1641)
                     /locus_tag="P5516_09135"
                     /inference="COORDINATES: protein motif:HMM:NF018585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phage baseplate assembly protein"
                     /protein_id="HRT92630.1"
                     /translation="REYFQHYGFTSRPLPGAEIIIIREGNHFVSIASDDRRYRVAVEA
                     GEVAIYTDEGDKVHFKRGKNIEVVCGNKLKATVANEVEVFTKTAIINASVGVQVASPS
                     ILLGSQGTTYALVDQRLLNLYNNHSHGIDGTPVAKLSLGNVCTSITKAG"
BASE COUNT          354 a          449 c          422 g          416 t
ORIGIN      
        1 cacggttgtt tctttattgt cggaaacgtt ttctacgata tcgcccacac ccacgggata
       61 agtcgcgtcg ttaaaagcgc cggcggaatc aattaatttg cactcttccg ctgaagtatt
      121 tgtgcccgtc tggcagtgaa taatgtacgg ttcgccggca aaagggaata tatccgcgtt
      181 gaggctaatt tgtgtgttgc tatccacgct ggccactgtt gttctggtct ggcgcagcgg
      241 gttttccaca atatcgcctg ccacaacggc gtggccgccg gtaaaagtcg cgccggaatc
      301 aaccagtttg ccgctggccg cgctggttac aacgccgatg cgtgcagata agctgggtat
      361 ttcgctgccg gtcgtttggc tattggccag caccacaacc tccacatgcc ccagcgcgcc
      421 ggccgggatg ggataacacc aggcccgcgc aacgttattt acctcgcgcg cccattttac
      481 gtaatcaaat ttgttgccgc ctgcgggtgg gcggcgtata tcatccagca agcgcgataa
      541 aagcgcagca tcggattcgc catacgtgcg ggttatgccg cgcacccagg catggtgctc
      601 tagttgttgg gtatccgccg tgtccgggaa tatctgtttg gcgatccaat cctgatactt
      661 atacaacccc cacagcgccg aggccaggca cgcggattta atgaatatca acgatccggc
      721 tgatgtgtct gcgctgggga actggttgcg gtaatcggtt aagatttcat ttaaaagttc
      781 gtcgaacgac ttttggaacg acattaaatc acctccacaa aagttgtgaa cacaacctga
      841 tccccgttcg cctttgtaac ggttatcaat aatttcaggc ggtgcggatc ctgcgcgcgg
      901 tctcgctggg ataaaacatc cacggccgtg gccttacccg tgtcgatgag ccattggagg
      961 gcttctttac aatattcttc cgccagcgcc gctgtctgtt ccgtgttttt gctgcgcgcg
     1021 aggagatgca ggcgggagcc gaagctggga tcctgaaacc acgatccacg ccggacaacc
     1081 aggctcaaaa atatattatt cagcaggttg ccgttctggc tcttatcaaa cgtcatatat
     1141 ccgctgcccg tctggtcctc gatagtgata gcaaaatcca ttttttaccc cgcttttgtt
     1201 atgctggtgc aaacgttgcc cagactcaac ttggcaaccg gcgtgccgtc tatgccgtgc
     1261 gagtggttgt tgtataaatt cagcaggcgc tggtccacca gcgcatacgt tgttccctga
     1321 ctgcccagaa ggatggaggg gcttgcaacc tgcacaccca ccgaggcatt aataattgca
     1381 gtttttgtaa agacttccac ttcgttcgcc actgtggctt ttagcttgtt gccgcagaca
     1441 acctcgatat ttttcccgcg tttgaaatga actttatcgc cctcatcggt atatattgcg
     1501 acctcgcccg cttccacggc gacgcgataa cgccggtcgt cggaggcgat cgacacaaaa
     1561 tgatttccct cacggataat aattatctct gcacccggaa ggggccgcga agtaaagccg
     1621 taatgctgaa aatattctct g
//
LOCUS       DAOTLW010000126         1632 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_60687_length_1632_cov_13.800888, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000126 DAOTLW010000000
VERSION     DAOTLW010000126.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1632)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1632
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_60687_length_1632_cov_13.800888"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..287)
                     /locus_tag="P5516_09140"
     CDS             complement(<1..287)
                     /locus_tag="P5516_09140"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006306 - DNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA methyltransferase"
                     /protein_id="HRT92631.1"
                     /translation="MSVQLYLGDCLEVMRTLDENSIDTCITDPPYELGFMGKKWDSSG
                     IAFQPETWQAVYRVLKPGAIMLAFGGTRTYHRIACAIEDAGFEIRDTIAWV"
     gene            complement(287..1615)
                     /locus_tag="P5516_09145"
     CDS             complement(287..1615)
                     /locus_tag="P5516_09145"
                     /inference="COORDINATES: protein motif:HMM:NF016988.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRT92632.1"
                     /translation="MMIENYIYSALSSMLRETETDRLESFQRRREAYVSGGEKPLKTD
                     SGGFDDSVRMNYARMIVDKGVAFLFGKDVRFELAEGDVTPQEEWLDAVWMANRKMSLL
                     QKLATNGGIYGTAFLKIHAQPGQMPKLVVVDPETVSVALDPMDVDSVLRYTIRYQTLD
                     PQTDKIISVRQVIAQDGLQWQITDEIGNVDGGTWQTVSVQTWPYRFAPMLHCQNLNAP
                     NAFWGISDIEDDVLEVINKESYTVSNILKILRFHAHPKTWVRGVSAAQIKVNPDNMIV
                     LPGETATIQNLEMQSDLASSIAMHRELVQFIHGLSRIPEVSTGKLDNVGALSGVALEI
                     LYQPLLEKTQAKRVTYGDMLVELNRRLLAIGGYGEENYTELRWQDLLPKDKVAETNAA
                     LVLKQLGVSGDTLVQQFGYDPDLEREKRAKESDLGQALLQAFDRGEAQAE"
BASE COUNT          352 a          507 c          377 g          396 t
ORIGIN      
        1 tatacccaag cgatagtgtc acggatttca aagccagcgt cctcaatagc gcaagcaatt
       61 cggtgatagg tgcgagtgcc gccgaaggca agcataatcg cgcctggttt cagcacacgg
      121 taaacagcct gccatgtttc gggctgaaag gcaattccag acgaatccca tttcttaccc
      181 ataaagccca gctcataagg cggatcggtt atgcaggtgt caatgctatt ctcgtcaagc
      241 gtacgcatga cttccaaaca gtcgcccaaa taaagctgta cgctcattac tcagcctgtg
      301 cttcgccgcg gtcaaacgct tgcagcaggg cttgcccaag atcgctttct ttcgcccgct
      361 tctcacgctc cagatcaggg tcgtatccaa actgctgaac cagcgtatcg ccgctcacgc
      421 ccaattgttt cagaaccaaa gccgcgttcg tttccgcaac tttgtcttta ggcagcagat
      481 cctgccagcg caattctgtg tagttttcct cgccatagcc gccgatcgcc agcagccggc
      541 ggttcagctc caccagcata tcgccgtatg tcacccgctt cgcctgcgtc ttttccagca
      601 gcggctgata cagaatttcc aaagccacgc cagaaagcgc gccaacgtta tccagcttac
      661 ctgttgaaac ttccggaatg cgcgacaacc cgtgaataaa ttgcaccaat tccctgtgca
      721 tcgcaatact ggaagccagg tcgctttgca tctcaaggtt ctggatggtt gctgtttcgc
      781 ctggaagcac gatcatgttg tccggattca cttttatttg tgccgcgctc acgccccgca
      841 cccacgtttt cgggtgcgcg tggaagcgca atattttcag gatattcgag actgtgtaag
      901 actctttgtt gataacttcc agcacatcgt cttcaatgtc gctgataccc caaaacgcgt
      961 taggcgcgtt caggttctgg cagtgcaaca tcggcgcgaa gcggtatggc caagtctgca
     1021 cactgaccgt ctgccacgtt cccccatcca cattcccgat ttcgtccgtg atctgccatt
     1081 gcagcccatc ctgcgcgatc acctgtcgga cggagataat tttatccgtc tgcgggtcaa
     1141 gcgtctgata ccggatcgtg taccgcaata cgctgtccac atccatcggg tcaagcgcaa
     1201 cgcttacggt ttcaggatca accacaacca gcttcggcat ctgtccgggc tgcgcgtgta
     1261 tcttcaggaa cgccgtccca taaatgccgc cgttggtcgc cagcttctgg agcaatgaca
     1321 tcttccggtt cgccatccaa accgcgtcca gccattcctc ttgcggcgta acatcgccct
     1381 ctgcaagctc gaaccgtacg tccttgccga ataggaacgc aacgcccttg tccacgatca
     1441 tgcgcgcgta gttcatccgg actgagtcgt caaagcctcc gctgtccgtc tttagcggct
     1501 tttcacctcc ggacacatac gcctcacggc ggcgctgaaa gctctccagc ctgtcagttt
     1561 ctgtctcccg taacatactt gagagcgccg aataaatgta attttcgatc atcatcaccg
     1621 cctatttgta aa
//
LOCUS       DAOTLW010000127         1621 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_61287_length_1621_cov_7.365262, whole genome shotgun sequence.
ACCESSION   DAOTLW010000127 DAOTLW010000000
VERSION     DAOTLW010000127.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1621)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1621
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61287_length_1621_cov_7.365262"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
BASE COUNT          371 a          443 c          442 g          365 t
ORIGIN      
        1 ctgaaggcgt gccacatagc agacacgaaa gatggtttcc attgcaaggc aacaggaagc
       61 gaccaatatc aatgagtgac cggttacgat gcggagtaaa cggtcaccga ttgataattg
      121 gccggtccaa gggacgctga gaccgcttgg attcattggc ggcggcgttg gggcgaggac
      181 tcacgactca taaggcatga tcccatggat tggacaactg agacgccctg gaccgtgggg
      241 aatgacacct tggactcagg ggactggttg gccgacgccg gggactcctg gaggcccctc
      301 ctgcgcgtga gacaagcaat ctgcgggaat ctgccccaat cggcgaaaaa accggcggcg
      361 tggctcggca gcgccgcgca gcggcgatgc gagacatgcc gctaataatc tgcgccaatc
      421 tgcgtaatct gcggataaaa aatgtctgtt atcaatcggt gaccgcttac cctgacatga
      481 ccaccttgtc cacctttcgt ccttgtatcg ccggagtggc aggctacgtt tttgccgtgt
      541 ttcagggcgc tgcgcgagtt tagcccatct ttctcacttt agcccccttt tgaggctaaa
      601 aaggcacaac aaacttttca catctccccc cgggtcgcga acgaacgagt ggctcaggcc
      661 ccgccagccg gtgacggctc cacgaccact ttccgcgcag gcagcgcgtc cctgcctatg
      721 ccgggagcgt cgtgggtcaa ctgaaaaaga agaacactat tggggcttgt cttttcgcga
      781 aagtacattt tgtgcctttc cggcaaatgt tcctaatacg taccttcaat tagactagcg
      841 aatggattga aaaaatcaag cgaaaactcg cgttccaaaa aggcacaaca aaagcgtttt
      901 cacccgctcc cacgcacgaa gccgacgcgg cgggcgccgg tcccacgcag gaattttttt
      961 ttgaaaaaaa acaaagtgag aaagatgggc taatatggcg tgttcttttt tcaactcttt
     1021 ggagtggagg caagcatgat ggtgtaaccg gtcacccatt gataattggc cgttttctgt
     1081 tgccttgcaa tggaaaccat ctttcgtgtc tgctatgtgg cacgccttca gcgtgctgtt
     1141 tcgggtgggg gcttccctgg ggttgcgcca cgggcgcaac cccaggctcc tatgaaactc
     1201 gccgcgcgag tcgtctgaac tcacaacgaa gaacggtcca atcacaatcg tgacaaggca
     1261 gctgaaatca tccagaagca caaaatgctg ttacttggcg attttttggc ttgtcagggg
     1321 cgattttttg gctatttttc acgcagtcct ttaaattcca gcccacccac cctagggggc
     1381 ccccatccta ataaggtcgc gcgcgcgagg gacagactgc gctcaggctg gggccacatc
     1441 gtatccatgg ctcttatgaa atccgccgcg tggtcagcca ccactatgtg tcgcgagttt
     1501 cctgcaaatt gtccccggtg tattccccac cgggatggct actggtatgt ggtgcccttt
     1561 tagggcactg gtatgttttg cgccttcagc gctgaaaacc aaagaggcga caggaaaacg
     1621 c
//
LOCUS       DAOTLW010000128         1616 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_61569_length_1616_cov_10.261371, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000128 DAOTLW010000000
VERSION     DAOTLW010000128.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1616)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1616
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61569_length_1616_cov_10.261371"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            12..128
                     /gene="rpmC"
                     /locus_tag="P5516_09150"
     CDS             12..128
                     /gene="rpmC"
                     /locus_tag="P5516_09150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRT92633.1"
                     /translation="MTGQLTDTSRFKATRRMIARYETILNERANEGKEGKDE"
     gene            121..411
                     /gene="rpsQ"
                     /locus_tag="P5516_09155"
     CDS             121..411
                     /gene="rpsQ"
                     /locus_tag="P5516_09155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRT92634.1"
                     /translation="MNNRRRLIGTVTSAKMPKTVSVEVTRTYQHPLYHKVVRGSKIYK
                     AHDELNCHVGDKVRIVESAPLSRTKHWIVEEILKVEVREEDDLSQISEGEQA"
     gene            408..779
                     /gene="rplN"
                     /locus_tag="P5516_09160"
     CDS             408..779
                     /gene="rplN"
                     /locus_tag="P5516_09160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRT92635.1"
                     /translation="MIQQETRLVVADNTGAKELLVIQVVGGTRRRYGYVGDIVVGTVK
                     SATPQASVKKSEIVKAVIVRTSKEYRRPDGSSISFDDNAAVILDSDGVNPRGTRIFGP
                     VARELREKGFMKIVSLAPEVL"
     gene            801..1235
                     /gene="rplX"
                     /locus_tag="P5516_09165"
     CDS             801..1235
                     /gene="rplX"
                     /locus_tag="P5516_09165"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRT92636.1"
                     /translation="MMMKIRKGDRVRVMRGDAEDKGKVGEVIRVLPKENRIVVQGVNL
                     VKKHQRQTQSGGKTIPSGIREFEAPIHISNVQLVTEASEKSAGKEKKAAKPAKKPTEK
                     PAAAKPAKKAAEKQASKAAEGKPAARRRSAAKPAADESGSEN"
     gene            1253..>1616
                     /gene="rplE"
                     /locus_tag="P5516_09170"
     CDS             1253..>1616
                     /gene="rplE"
                     /locus_tag="P5516_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRT92637.1"
                     /translation="MNRLKEQYKNEIAPALFKELGLDNVMQTPRIEKVVVNIGVGEAL
                     DNPKALEAAVNDLTIITGQKPVVTAAKKAISNFKLREGRQIGVKVTLRGEKMWAFLDR
                     LINIALPRVRDFRGVSAEA"
BASE COUNT          456 a          349 c          464 g          347 t
ORIGIN      
        1 gctttcaggc ggtgaccggt cagctgacgg ataccagcag atttaaagcc acccggcgta
       61 tgatcgcgcg atatgagacc attctcaacg agcgcgcaaa cgaggggaag gaaggtaaag
      121 atgaataata gacggcgact tatcggcacg gttaccagtg ccaagatgcc taaaactgtg
      181 agcgtagagg taacccgcac ctatcagcat ccgctgtatc acaaggtggt gcgtgggagc
      241 aaaatttaca aggctcatga cgaactaaat tgccatgttg gcgataaggt ccggattgtt
      301 gaatctgcgc cgctttcgcg tacgaaacat tggattgtgg aagaaatcct caaggttgaa
      361 gttcgtgaag aagacgacct cagtcagatt tctgaaggag agcaagcatg attcagcagg
      421 agactcgttt ggttgttgcc gataacacag gcgccaaaga gctattggtt attcaggtag
      481 tcggtggtac ccgccggcgc tatggatacg taggtgacat tgtggtaggc acggttaaaa
      541 gcgccacacc acaggcgtct gtgaagaaga gcgaaattgt taaagccgtg attgtgcgca
      601 cgagcaaaga ataccgccga ccggatggtt ccagcatttc ttttgatgat aatgctgcgg
      661 taatccttga ctctgatggc gtaaatccac gtgggacgcg tatttttggc cctgtggcac
      721 gtgaactgcg cgaaaaaggc tttatgaaga ttgtttcatt ggctccagaa gtgctgtagc
      781 cgttgatgtc aggagtgtca atgatgatga agatacgtaa aggtgatcgc gttcgggtga
      841 tgcgcggcga tgccgaagat aaaggcaaag ttggcgaggt gatccgggtg ctgcctaagg
      901 agaatcgaat tgtcgtgcag ggtgtgaatc tggtgaaaaa acaccagcgg caaacccagt
      961 ctggcggcaa aacaatccca tctggcatcc gcgagttcga agcgccgatt cacatttcga
     1021 atgtgcaatt ggtcactgag gcaagtgaga aatcagccgg caaggaaaag aaagctgcca
     1081 aacccgcgaa aaaacctact gagaagccgg ctgctgccaa acctgcgaag aaggctgccg
     1141 aaaagcaagc ttcaaaagct gcggaaggca aaccagcggc tcgtagacgc agcgctgcca
     1201 aacctgccgc agatgaaagc ggttctgaga attaagatgc aggtgagcat aaatgaatag
     1261 attaaaagaa caatacaaaa atgaaatcgc tccggcgctt ttcaaagagt tgggtctgga
     1321 taatgtgatg cagactccgc gcattgaaaa agtggtcgtg aatattggtg taggcgaagc
     1381 tttggacaac cccaaggcac ttgaagccgc tgtgaatgat ctcacgatca tcactggtca
     1441 gaaaccagtg gtaacagcag ccaagaaagc catttctaac ttcaaattgc gtgaaggtcg
     1501 ccaaattggc gtcaaagtta ctttacgcgg cgaaaagatg tgggcgttct tggaccggct
     1561 gatcaatatc gccttaccgc gtgtgcgaga ctttcgtggt gtctctgcgg aggcgt
//
LOCUS       DAOTLW010000129         1615 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_61627_length_1615_cov_8.362821, whole genome shotgun sequence.
ACCESSION   DAOTLW010000129 DAOTLW010000000
VERSION     DAOTLW010000129.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1615)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1615
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_61627_length_1615_cov_8.362821"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..306
                     /locus_tag="P5516_09175"
     CDS             <1..306
                     /locus_tag="P5516_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT92638.1"
                     /translation="TTNSGKKIYFAGDTGLFGDMRLIGEEGVELAALPIGDNYTMGPD
                     DALRAVKLLQPKVVIPIHYNTWPLIKQDPQAWKQRVEAETQTKCVVLAPGGTYQVEA"
     gene            311..931
                     /locus_tag="P5516_09180"
     CDS             311..931
                     /locus_tag="P5516_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite oxidase-like oxidoreductase"
                     /protein_id="HRT92639.1"
                     /translation="MKSSTEERPIKTGRIPPGQFLSKRFPVLHYGPIPPFDPETWTFR
                     VWGEVEKPLTLTWQEFSQLPRTTLTLDLHCVTKWSKLDTVWEGVSLKTLRELGLVIPT
                     ADAHYVIQHAEYGFTANLPIEVVFADNFLLATHYDGKPITPEHGAPLRGLVGSIPGRD
                     DLKDVYLWKGAKWLRGLEFVAQDRPGFWERAGYHNDADIWLEERTA"
     gene            complement(1019..>1615)
                     /locus_tag="P5516_09185"
     CDS             complement(1019..>1615)
                     /locus_tag="P5516_09185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92640.1"
                     /translation="DTIIQLNGLDENCNLITGTKILLGTVEAPALTETAQPTATLPPE
                     LITPTPTPEPGFAKICVVLFHDIDGTGTRTTGEDYLYGGVVSINDRLGKVSLTGTTVA
                     GNPDTTDPLCFENIPEGSYNITVAIPDGFNPTTVTSYPLEVKAGDQATIDFGAQQATA
                     PVQQEDTAGTRSPILGIVGVVILIAGLVLAFLTWRQHR"
BASE COUNT          341 a          413 c          482 g          379 t
ORIGIN      
        1 acgaccaatt ctggcaagaa gatctacttt gccggcgata caggtctctt tggggatatg
       61 cgcctgattg gtgaagaggg cgtggagctc gctgccctgc cgattggtga taactacacc
      121 atgggccccg atgatgccct gcgcgcagtg aagttgctgc agcctaaagt ggtcatccca
      181 attcattaca acacctggcc gctcatcaag caagaccctc aggcttggaa acagcgtgtg
      241 gaagctgaaa cccagaccaa gtgcgtcgtg ttagcacctg ggggaactta tcaggtggag
      301 gcgtgaaccc atgaagtcaa gcacagagga acgtcctatc aagactgggc gcataccacc
      361 ggggcaattt ctttccaaac ggttcccggt gctgcattat ggacctattc cgccttttga
      421 ccctgaaact tggactttcc gcgtgtgggg agaggtggaa aagccactca cactcacttg
      481 gcaggagttc agccagctgc cgcgcacaac gcttaccctt gacctgcact gtgtgacgaa
      541 atggagcaag ctggataccg tctgggaagg cgtcagcctt aagaccttac gggagctggg
      601 tctcgtcatc ccaacagcgg atgcacacta tgtcattcag cacgcggaat acggctttac
      661 tgccaacctg ccgattgaag tcgtttttgc ggataacttc ttgcttgcga cccattacga
      721 cggcaagccg attacacccg aacatggtgc gcctttgcgc ggattggtcg gctccatccc
      781 cgggcgcgat gatctcaagg atgtctacct ctggaagggc gctaagtggc tgcgcgggct
      841 ggaattcgtg gcgcaagacc gccctggctt ttgggagcgg gctggctatc ataatgacgc
      901 tgacatttgg ttggaggaac gcacagccta accatccccg aggtttttgg aaaaatagaa
      961 acaccgccgt taggaatatt cacggcggtg ttttattact gcaagattta gctggtactt
     1021 acctgtgctg ccgccaggtg aggaatgcta ataccaagcc ggcaatgagg attaccacgc
     1081 caacgatgcc aagaatgggt gagcgcgtgc ctgcggtatc ttcttgttga acgggggcgg
     1141 tagcttgttg tgcaccaaaa tcaattgtgg cttgatcgcc cgctttcacc tccagcgggt
     1201 agctcgttac tgtggtgggg ttgaagccat cgggtatggc aaccgtgatg ttatagctgc
     1261 cctcggggat gttctcgaag cataatgggt cggtagtatc tgggttgcct gctaccgttg
     1321 tgccggtcag cgagacctta cccaggcggt cgttgatgct aaccacgccg ccgtagagat
     1381 aatcttcgcc ggttgtgcgc gtgccggtgc cgtcgatatc atggaatagc accacgcaga
     1441 tcttggcaaa ccccggttct ggggtgggcg tgggtgtgat caactcgggt ggcagggtag
     1501 ccgtgggctg ggcggtttcg gtgagggctg gggcttcaac cgtgcctagt aaaattttcg
     1561 tgccggtaat caggttgcag ttttcatcca agccgttgag ttggatgatg gtatc
//
LOCUS       DAOTLW010000130         1597 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_62597_length_1597_cov_9.269780, whole genome shotgun sequence.
ACCESSION   DAOTLW010000130 DAOTLW010000000
VERSION     DAOTLW010000130.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1597)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1597
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62597_length_1597_cov_9.269780"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..878)
                     /locus_tag="P5516_09190"
     CDS             complement(<1..878)
                     /locus_tag="P5516_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase"
                     /protein_id="HRT92641.1"
                     /translation="MMRYVFSSMRPRFLLLALACVFLGTAAAIWAVGKVNPWYVLLAF
                     IGGLAAHACVNAFNDYTDIRSGLDFRTNRTPFSGGSGTFVEVPEKVHLALWTTIGTAL
                     ITVVVGIYFALAVGWIILPIGILGLIVILTYTQWLNKQPLLCLLAPGFGFGTLMVLGT
                     FYVLTGRITWSAVLTSSIPFFLVSNLLLLNQFPDVEADTTVGRKHYPILVGRKTSAII
                     YGCFLAATYLAIILGVMLRITPAWTLLGLLTAAIALPTALKVLQHAENIPDLVPSMIQ
                     NVLLNLVTPLLMGVGF"
     gene            complement(932..>1597)
                     /locus_tag="P5516_09195"
     CDS             complement(932..>1597)
                     /locus_tag="P5516_09195"
                     /inference="COORDINATES: protein motif:HMM:NF024703.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HRT92642.1"
                     /translation="DELVLGSPFDYENSTLLYLVNDIPEPAEASAYQREVESAIIRLA
                     KATGGRMLVLFTSYAQLRKTAQAIGPAMADAEIQVYEQGEGASTNILLENFRGADKAV
                     LLGTRAFWEGVDIPGEALSVLVIVKLPFDVPSDPIIAARAETFEDPFNEYNLPEAILR
                     FRQGFGRLIRSQSDRGVVAILDKRVLTKKYGRMFLDSLPECTRRVASSLELPAAAARW
                     LNI"
BASE COUNT          399 a          417 c          463 g          318 t
ORIGIN      
        1 aagaaaccaa cacccatcaa cagcggcgtt accaggttga gcagcacatt ttgaatcatc
       61 gagggcacca gatcggggat gttctctgcg tgttggagca ccttcagcgc ggttggcagc
      121 gcgatggctg ctgtcagcaa gccgagcagc gtccatgccg gggtgattct gagcatcaca
      181 cccaggatga tggcaagata cgtggctgct aagaaacaac cgtagatgat ggcgctggtt
      241 tttctgccaa cgaggatggg gtaatgcttt ctacccaccg tggtatccgc ttctacatca
      301 gggaattgat tcaacaggag caggttgctg accaggaaaa aaggaataga agatgtaagt
      361 accgccgacc aggtaatacg ccccgttagc acgtagaagg ttcccaacac catgagggtg
      421 ccaaaaccga accctggcgc gagcaggcat aagaggggtt gtttgttgag ccattgggta
      481 taggttaaga tgacaatcag ccccaggatg ccaatgggca ggatgatcca gccgaccgcc
      541 agcgcgaaat agatgccaac caccacggta atcagcgcag tgccgatggt ggtccataac
      601 gccaggtgta ccttttcggg cacttcaacg aaggttccgc tgccaccgct gaatggggtg
      661 cggttggtgc gaaaatccaa accgctgcga atatcagtgt aatcgttaaa ggcgttcacg
      721 caggcgtggg ctgcaagacc gccgatgaat gccagtagaa cataccaggg gttcacctta
      781 ccaactgccc agatggctgc cgccgttccc aggaaaacgc aggcgagggc gagcaagaga
      841 aaccgcggac gcatggaact gaaaacgtat ctcatcatat tgctccaata aagggattgt
      901 ggtcgtgtaa caataacacc ggaaaaaggg tttagatgtt caaccaacgg gcagccgcgg
      961 cgggtagttc caggctggaa gccaccctgc gggtacattc cgggagtgaa tcgaggaaca
     1021 tacgaccata cttcttggtg agcactcgct tatccaaaat ggccaccacg ccgcggtcag
     1081 attgggagcg gattaaacga ccaaagccct gcctgaaacg caggatggct tctggcaggt
     1141 tgtactcgtt gaatgggtct tcaaaggttt ctgcgcgggc agcgatgatg gggtcagaag
     1201 ggacatcaaa gggcagtttt acgattacca gcacagaaag cgcttcgccc ggaatatcga
     1261 cgccttccca gaatgcgcgc gtacccagca aaacggcttt atcggctcca cggaagtttt
     1321 ccagcaggat gttggtggaa gcaccctcgc cctgttcata gacctgtatc tccgcgtccg
     1381 ccatcgcggg tccgatcgcc tgggcggtct ttctcaattg agcgtacgag gtgaacagca
     1441 ccagcatacg cccgccggtg gctttagcca ggcggatgat ggcgctctcc acttctcgtt
     1501 gatacgcgct ggcttcagcc ggttcgggga tgtcattcac caggtagagc agggtggagt
     1561 tttcgtaatc aaacggagaa cccagcacca gttcatc
//
LOCUS       DAOTLW010000131         1591 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_62920_length_1591_cov_12.136068, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000131 DAOTLW010000000
VERSION     DAOTLW010000131.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_62920_length_1591_cov_12.136068"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /locus_tag="P5516_09200"
     CDS             <1..504
                     /locus_tag="P5516_09200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92643.1"
                     /translation="AAGVPCLLVGGLAVNHHGYTRNTLDIDWMIVGAHRDVVRDVMKK
                     AGFTNVAIHENVMFCQRPGSAWRIDFLFVDDDTMAQLLSRSERTVVQGKTVSVPSLKD
                     LIAMKLFSLAQNPARRMGKDLPDIVHLTVLNGLDLEKDIHPLAVRYASEAIFDMVSEQ
                     VKALVNE"
     gene            1341..>1591
                     /locus_tag="P5516_09205"
     CDS             1341..>1591
                     /locus_tag="P5516_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024571.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT92644.1"
                     /translation="MALVERFLQKPRRSSFLFGPRGTGKTTWLRQVFGDVPTLDLLDA
                     ETYRSYVARPERLREWLAAQGRPAWVVIDEVQRVPALLDV"
BASE COUNT          365 a          384 c          466 g          376 t
ORIGIN      
        1 gccgcaggtg tgccgtgttt gctggtcggt ggtttggctg tcaaccacca cggttacacg
       61 cgtaacacct tggacatcga ctggatgatt gtgggtgcgc acagggatgt tgtccgggat
      121 gtgatgaaaa aagcggggtt cacgaatgtg gcgatccatg aaaacgtcat gttctgccag
      181 cgtccgggat ccgcttggcg cattgatttt ctgtttgtgg acgacgacac catggcccag
      241 ttgctatcga ggtcggagcg caccgtcgtg cagggaaaga cggtcagcgt gccgtcgctg
      301 aaagatctga tcgccatgaa gctcttttct ctggcgcaga atcccgcgcg gcggatgggc
      361 aaagacttgc cggacattgt tcacctgact gttctcaacg ggctcgacct agaaaaggac
      421 attcaccccc tcgccgtccg ttacgcttcg gaagccattt tcgatatggt gtcggaacaa
      481 gtgaaagccc tggttaatga ataactaata gttaataacg aatagttggg tgcgagctgt
      541 ttgaaaaatc tgtggatgaa accttgaaac ctgaaaccag gaaccaacca gtatgaactt
      601 ccccgtcata tcagaatgcg cggcgcgtga agcgctccgt tcgcttccga ggctggacat
      661 ggacgagtat gtggcctttc tgtccgagtc catgtcctgg gttgatccgg aaaaggccgc
      721 caagcaaaag gatcttgaag agcgcatcac cgtgcccttc cgtatacgtg acaagtaagc
      781 ggcgcttgtt taaccacgga atacactgaa cacacggaaa ggcgtggctc agttttttgg
      841 aaccgccaag aggccaagaa cgccaagggc aagccttcaa ttttcatgaa gtttcatgtg
      901 tttcatggtt cggattcgtt taacgcagag gcgcagagac gcagaggtgg gggaagttca
      961 ttagttcatt agtgcattag tgcgttagtt tgttttggca tggctcagtt tttggaaacc
     1021 acagagagca cagaggcgca gaggtttttt aggggctgtt ccagccgcag gtcgcgggtc
     1081 gaatgtcgcg ggtcaacctc tcttgttgcg accacgcagt ggttcgcaat gatgagtgta
     1141 aaagcgctgc ccggttgttt cacgcgaagg cgcgaagacg cgaagggtgg agggcgagtg
     1201 tccccacgag ccgtacacaa tcaatcatgt taatcatgaa atcatatcta aaaactacgt
     1261 agccatgccc gattattcat tattcgttat tcgttattac cttccaaaac gccatcgcta
     1321 atgtaaaaaa agaagcataa atggcattag tagagcgatt tttacagaaa ccccggcgca
     1381 gttcgtttct tttcggaccg cgcggcacgg ggaaaacgac gtggctgcgg caggtgttcg
     1441 gagatgttcc gacgctggat ctgctcgatg ccgagaccta ccgctcgtat gtggcgcgtc
     1501 ccgagcgttt gcgggagtgg ctggccgcgc aggggcgtcc cgcctgggtg gtcatcgacg
     1561 aagtgcagcg cgttccggcc ctgctggatg t
//
LOCUS       DAOTLW010000132         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_63716_length_1577_cov_14.934297, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000132 DAOTLW010000000
VERSION     DAOTLW010000132.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63716_length_1577_cov_14.934297"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..364)
                     /gene="rplE"
                     /locus_tag="P5516_09210"
     CDS             complement(<1..364)
                     /gene="rplE"
                     /locus_tag="P5516_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HRT92645.1"
                     /translation="MNRLKEQYNKEIAPALFKELGLDNVMQTPRIEKVVVNIGVGEAL
                     DNPKALEAAVNDLTIITGQKPVVTAAKKAISNFKLREGRQIGVKVTLRGEKMWAFLDR
                     LINIALPRVRDFRGVSAEA"
     gene            complement(361..777)
                     /gene="rplX"
                     /locus_tag="P5516_09215"
     CDS             complement(361..777)
                     /gene="rplX"
                     /locus_tag="P5516_09215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HRT92646.1"
                     /translation="MMMKIRKGDRVRVMRGDAEDKGKVGEVIRVLPKENRIVVQGVNL
                     VKKHQRQTQSGGKTIPSGIREFEAPIHISNVQLVTEASEKAASKEKKAAKPVKKSAAK
                     PAAKASETKPTPRRRGAAKPAADESGSEKLGAGENK"
     gene            complement(799..1170)
                     /gene="rplN"
                     /locus_tag="P5516_09220"
     CDS             complement(799..1170)
                     /gene="rplN"
                     /locus_tag="P5516_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007073026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HRT92647.1"
                     /translation="MIQQETRLVVADNTGAKELLVIQVVGGTRRRYGYVGDIVVGTVK
                     SATPQASVKKSEIVKAVIVRTSKEYRRPDGSSISFDDNAAVILDSDGVNPRGTRIFGP
                     VARELREKGFMKIVSLAPEVL"
     gene            complement(1167..1457)
                     /gene="rpsQ"
                     /locus_tag="P5516_09225"
     CDS             complement(1167..1457)
                     /gene="rpsQ"
                     /locus_tag="P5516_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HRT92648.1"
                     /translation="MNNRRRLIGTVTSAKMPKTVSVEVSRTYQHPLYHKVVRGSKIYK
                     AHDELNCHVGDKVRIVESAPLSRSKHWIVEEILKVEIREEDDLSQISEGEQA"
     gene            complement(1450..1566)
                     /gene="rpmC"
                     /locus_tag="P5516_09230"
     CDS             complement(1450..1566)
                     /gene="rpmC"
                     /locus_tag="P5516_09230"
                     /inference="COORDINATES: protein motif:HMM:TIGR00012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HRT92649.1"
                     /translation="MTGQLTDTSRFKATRRMIARYETILNERAHEGKEGKDE"
BASE COUNT          341 a          447 c          340 g          449 t
ORIGIN      
        1 acgcctccgc agagacacca cgaaagtctc gcacacgcgg taaggcgata ttgattagcc
       61 ggtccaagaa tgcccacatc ttttcgccgc gtaaagtaac tttgacacca atttggcggc
      121 cttcacgcaa tttgaagttg gaaatggctt tcttggctgc tgtaaccact ggtttctgcc
      181 cagtgatgat cgttagatca ttaacagcgg cttcgagcgc tttggggttg tccaaagctt
      241 cgcccacacc aatattcacg accacttttt caatccgcgg ggtctgcatt acattatcca
      301 gacctaactc cttgaaaagc gccggagcga tttctttgtt gtattgttct tttaatctat
      361 tcatttattc tcacctgcac ctaatttctc agaaccgctt tcatctgcgg caggtttggc
      421 tgcaccacgc cttcgaggag ttggcttggt ttcacttgct ttagcagctg gcttcgcagc
      481 cgatttcttg acgggtttgg cagctttctt ttccttgctg gctgctttct cacttgcctc
      541 agtgaccaat tgcacattcg aaatgtggat tggtgcttcg aactcgcgga tgccagatgg
      601 gattgttttg ccgccagact gggtttgccg ctggtgtttt ttcaccagat tcacaccctg
      661 cacgacaatt cgattctcct taggcagcac ccggatcacc tcgccaactt tgcctttatc
      721 ttcggcatcg ccgcgcatca cccgaacgcg atcaccttta cgtatcttca tcatcattga
      781 cactcctgac atcaacggct acagcacttc tggagccaat gaaacaatct tcataaagcc
      841 tttttcgcgc agttcacgtg ccacggggcc aaaaatacgc gtcccgcgtg gattcacgcc
      901 atcagaatca aggattacag cagcattatc atcaaaagat atgctggaac catccggtcg
      961 gcggtattcc ttactcgtgc gcacaatcac ggctttgaca atttcgcttt tcttcacaga
     1021 tgcctgcggc gtggcgcttt taaccgtgcc taccacaata tcacctacgt atccatagcg
     1081 ccggcgggta ccgccgacta cctggataac caacagctct ttggcgcctg tgttatcggc
     1141 aacaaccaaa cgagtctctt gctgaatcat gcttgctctc cttcagaaat ctgactcagg
     1201 tcatcttctt ctcgaatttc aaccttgagg atttcttcca caatccaatg tttgctacgc
     1261 gaaagcggcg cagattcaac aatgcggacc ttatcgccaa catggcaatt aagttcgtca
     1321 tgagccttgt agattttgct cccacgcacc actttatgat acagcggatg ctgataggtg
     1381 cggctgacct ctacgctcac agttttaggc atcttggcac tcgtaactgt gccaataagt
     1441 cgacgtctat tattcatctt taccttcctt cccctcgtgt gcgcgttcgt tgaggatagt
     1501 ctcatatcgc gcaatcatgc ggcgggtggc tttaaatctg ctggtatccg tcagctgacc
     1561 ggtcaccgcc tgaaagc
//
LOCUS       DAOTLW010000133         1577 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_63719_length_1577_cov_6.323259, whole genome shotgun sequence.
ACCESSION   DAOTLW010000133 DAOTLW010000000
VERSION     DAOTLW010000133.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1577)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1577
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63719_length_1577_cov_6.323259"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..630
                     /locus_tag="P5516_09235"
     CDS             <1..630
                     /locus_tag="P5516_09235"
                     /inference="COORDINATES: protein motif:HMM:NF024138.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HRT92650.1"
                     /translation="WQESSVASSHVGSMGGIMAVKRGEAHLGGIHLLDEASGTYNIPY
                     LRKYFPEGGVVLVECVQRTQGLMVPKGNPLGIKEFKDIVGKRYVNRQKGSGTRILCDF
                     LARQEGLDTSLIPGYEREEYTHTAVAAAIAAGTADTGLGILSAARIYDLDFVPICEEQ
                     YDLLIAENAMSLEMVQRLLAVLRGEEFARRLTQMGGYTLHHPGEVRPWN"
     gene            621..1091
                     /gene="moaC"
                     /locus_tag="P5516_09240"
     CDS             621..1091
                     /gene="moaC"
                     /locus_tag="P5516_09240"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001612668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HRT92651.1"
                     /translation="MELSHTDEQGNARMVDVSAKGDTLRKAIASAFIRMQPETLALIQ
                     SGGIAKGDVFATARIAGIMAAKRTPELIPLCHPIPLTFVGVELVPEQPDGVCIIATVE
                     AMTKTGVEMEALTAASVAALTFYDMCKAVDRGMQIERVQLEHKSGGKSGTYQRE"
BASE COUNT          361 a          425 c          457 g          334 t
ORIGIN      
        1 tggcaggaaa gctcggtagc ctcttctcat gtgggcagca tgggcggcat tatggcggtc
       61 aagcgcggtg aggcgcactt gggcggcatc cacctgctgg atgaagccag cggcacctac
      121 aacattcctt acctgcgcaa gtacttccca gagggcggtg tggtactggt ggaatgcgtc
      181 caacgcaccc aggggctgat ggtacccaaa ggaaatccgc tgggaattaa agaattcaaa
      241 gatattgtgg gcaagcgcta cgtgaaccgc caaaaaggca gcggcacgcg cattctatgc
      301 gactttttag cccgtcagga aggcttggat acgagcctca tccccggcta cgagcgcgaa
      361 gaatataccc atacggcagt ggcagcggcg attgcggctg gcacagctga tacgggtttg
      421 ggtatccttt cggctgcacg catttacgat ctcgattttg tgcccatttg cgaagagcaa
      481 tacgatttgc tcatcgcgga aaacgcgatg agcttagaaa tggtgcagcg gctgctcgcg
      541 gtgctgcgcg gggaggaatt cgcccgccgg ctcacccaaa tgggcggcta cacgctgcat
      601 catcccgggg aggtgcgccc atggaactaa gccatactga tgaacaaggc aacgcccgca
      661 tggtagacgt ttctgctaag ggagacacct tgcgcaaagc catagccagt gcctttatcc
      721 gcatgcagcc ggaaactctg gcgctcattc aaagcggcgg catcgcaaag ggagatgttt
      781 ttgccacagc gcgtattgct ggcatcatgg ctgctaagcg cacacccgag ctgatcccgc
      841 tctgccatcc cataccgctc accttcgtgg gtgtggagct agtgccggag cagccggatg
      901 gcgtgtgcat cattgctacc gtggaagcta tgaccaagac tggtgtggag atggaagcgc
      961 tcaccgcagc cagcgtggca gccctgacgt tttacgatat gtgcaaggcg gtcgaccgtg
     1021 gcatgcagat cgagcgggtg caactggaac ataaaagcgg cggcaaatcg ggcacctacc
     1081 agcgggagta accgcagggg tagccttcag ttcctttgtt ctcgccggtc tctacaatct
     1141 cagttctcgc cggtctctga gtgcgtagcc tacccgaagg gtaccgagcg gggaattttt
     1201 taatcgctct cgccggtctc ctgaccgagc gaggaagttg accgcaggtc tcctgttttc
     1261 ctggctttga tcgacgatta tgataatcag gtcatcagtt ttttgagacc taaaggtcag
     1321 ccacctttca tggtcaggag agtgcgaagc ctgcccgtag ggtaccatga aagaactaag
     1381 aattatctgt tctcgccggt ctccagaccg tgcgagcggt tttgaccgca ggtctccctc
     1441 attcattgag acccaaaggt cacgttccat tcatggtcag gagagtgcat agcctacccg
     1501 aagagtacca tgaaagaact aaggggtacc ataaaagaac aggtaaaccc tgcatcgaac
     1561 catatctttg accgaca
//
LOCUS       DAOTLW010000134         1574 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_63886_length_1574_cov_11.221198, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000134 DAOTLW010000000
VERSION     DAOTLW010000134.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1574)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1574
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_63886_length_1574_cov_11.221198"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..419
                     /locus_tag="P5516_09245"
     CDS             <1..419
                     /locus_tag="P5516_09245"
                     /inference="COORDINATES: protein motif:HMM:NF013245.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HRT92652.1"
                     /translation="VVPLLVVMLITQTTIHLTFLFLPISILLLTGFALGFGLLLSALA
                     IFFPDVAEMYQILLMAWMYLTPVIYPEEIIPAQFMTIYRINPMYWMLKMFRLPVYEGV
                     IPTFQDLWPALAWSVGMLIVGWLFFTSRSDEYAYRV"
     gene            397..1185
                     /locus_tag="P5516_09250"
     CDS             397..1185
                     /locus_tag="P5516_09250"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92653.1"
                     /translation="MNMLIESKPLLNQKTKLNSEPIITLQDVSVAYRVPKERIGTFKE
                     YAIRTLQRKVQFNKFLALKDVSLTINRGEVFGIIGNNGAGKSTMLKVISRVLRPTQGR
                     VLLYGKIAPLLELGAGFHPELTGRENVYLNGALLGYDQNAMDAVFEEIVEFSELREFI
                     DAPLRTYSSGMYARLGFAVATAHVPEILIVDEILSVGDEAFQRKCNERMQSYQEQGST
                     VLIVSHGLAKIQDMCNRVLWLDHGQVQLIGDPAEVIEAYRRSNQ"
     gene            1187..>1574
                     /gene="rfbC"
                     /locus_tag="P5516_09255"
     CDS             1187..>1574
                     /gene="rfbC"
                     /locus_tag="P5516_09255"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HRT92654.1"
                     /translation="MEFIPTAIPDVVLIVPRVFGDDRGFFLETYRQDVFEAACGPVNF
                     VQDNHSGSRQGTLRGLHYQLRQAQGKLVRAVAGEIFDVAVDIRQGSPTFGRWVGARLS
                     AENKHQLWVPAGFAHGFYVLSAWAEVF"
BASE COUNT          355 a          384 c          403 g          432 t
ORIGIN      
        1 ccgtggtgcc gctgctggtg gtcatgttga tcacgcagac aaccattcac ctgacgtttc
       61 tctttcttcc catttcaatc ttgctgctta ccggttttgc gcttgggttc gggttgctgc
      121 tttcagcttt agcgatattc ttcccagatg tggctgaaat gtatcagatc ctgctaatgg
      181 cgtggatgta cctcacccca gtaatttatc cagaggagat catcccagcg caatttatga
      241 ccatctaccg catcaacccc atgtattgga tgctcaaaat gttccgctta cccgtttatg
      301 aaggggttat ccccactttt caggaccttt ggcctgcgtt agcttggtca gtgggtatgc
      361 tgattgttgg ttggctattc tttacctcga gatcggatga atatgcttat cgagtctaaa
      421 ccattgctaa accaaaaaac caaactgaac agcgaaccca tcatcacgct gcaggatgtg
      481 tcagttgcgt accgtgttcc caaagagcga atcggaactt tcaaagaata tgccattcgc
      541 acgctgcaac gcaaggttca attcaacaaa ttcttagcgc tcaaagacgt cagcctgaca
      601 attaatcgcg gggaggtctt tggcatcatt ggcaataacg gtgctggaaa aagcacgatg
      661 ctcaaagtaa tctcacgcgt tttacgccca acgcaaggac gcgtgctgct ttatggcaag
      721 attgctccgc tgttagaatt aggcgccggc tttcaccccg agctcaccgg tcgggagaat
      781 gtttatctta acggtgctct tctgggctat gaccaaaacg caatggacgc ggtctttgag
      841 gaaattgtag aattttctga gctgcgtgag tttattgatg cccccttacg cacctactcc
      901 tcaggtatgt atgcgcgctt aggctttgct gtggcaacag cgcacgtgcc cgaaatcctg
      961 attgtggatg agatcctgag cgttggagac gaagcctttc aacggaagtg caatgagcgc
     1021 atgcaaagtt atcaagagca agggagtact gttttaattg tttcccatgg cttggcgaaa
     1081 attcaagata tgtgcaaccg tgttctctgg ctggatcatg gccaggtgca attaattggc
     1141 gatccggctg aagttatcga agcttaccgc aggagtaacc aataagatgg aatttatccc
     1201 taccgccatc ccggatgttg tgctgattgt cccccgcgtg tttggcgatg accgcggttt
     1261 cttcttagag acctatcggc aggatgtttt tgaggctgct tgcggtccgg ttaacttcgt
     1321 gcaggataat cattcgggct cacgccaagg caccttacgt gggctacact atcaactccg
     1381 ccaggcacaa ggtaagcttg tacgtgccgt cgctggagag atctttgacg tggctgtgga
     1441 tatccgccag ggctcgccaa cctttggacg atgggtcggg gcgcggcttt ctgcagagaa
     1501 taaacatcag ctgtgggttc cagcaggttt tgcgcacggt ttttatgtgc tcagcgcctg
     1561 ggcagaagta ttct
//
LOCUS       DAOTLW010000135         1537 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_66084_length_1537_cov_8.212551, whole genome shotgun sequence.
ACCESSION   DAOTLW010000135 DAOTLW010000000
VERSION     DAOTLW010000135.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1537)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1537
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_66084_length_1537_cov_8.212551"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            94..1416
                     /locus_tag="P5516_09260"
     CDS             94..1416
                     /locus_tag="P5516_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92655.1"
                     /translation="MPEIPAQAILEQLPVTELEQSLTTFLAPMMAVLPDRRLQAIVPL
                     CVRGIIGSESPVVTQIAQQVAREESGVWAAAKRVYRFLGNPHFSTAQLTQGLYDCSVQ
                     SIRAAAPAYVVVALDPVNFEKPYTTELEGVSTVYKSTPPPLTGKGRLTRGYPALTATV
                     VNTPVPATTYAHWFSYTAADFISENWEFDQAIATTDRLLPEFRRRYVGDAGLDDQKLF
                     AALSQDEFVIRVSHLERWVEVYNERLDRWEREQLQDLVATVPFTHTFSVTFTHARKKR
                     RTRVKVGWLTLRLPDTHQRLWAVVIYDPPLDRTLVLLTNVPLFTIADVRQVYDDWRLR
                     PRVEHGYRFDQEQGLDVEDMRVQSLEAMQRLFVLVLAAAQFVFFLINTWPQQAVLWVR
                     SLGGKLGLANDLDGPYLVLRGLSALIQALVTLSFLSISPFPHQDFTYG"
BASE COUNT          315 a          442 c          417 g          363 t
ORIGIN      
        1 ttggtgatta cccacaactc gaggctggaa gaagtttcat ctccgtggta agattgagtt
       61 agcgcaacca aaactcagcc aggaggtgaa gccatgccag agattcccgc acaagcaatt
      121 ttggagcaat tgcccgtgac cgaattggag cagagtttaa cgacgttctt agcaccgatg
      181 atggccgtat tgcccgatcg gcgcttgcaa gccatcgtgc ccctctgtgt acgcgggatc
      241 atcggcagtg aaagcccggt ggtcacgcag atcgctcagc aggtagcgcg cgaagaaagt
      301 ggggtgtggg cggccgccaa gcgggtctat cgtttcctgg gaaatcccca ctttagcacg
      361 gcgcaactga cccaaggctt gtatgactgt tccgtgcaga gcatccgggc ggcagcgcca
      421 gcctatgtag tggtagcgct ggatcccgtg aattttgaaa agccgtatac gacggagtta
      481 gaaggcgtaa gcaccgttta caagagcacg ccacccccgt tgaccggcaa agggcgcctc
      541 acgcgggggt acccggctct caccgccacc gtggtcaaca cgcccgtgcc ggccaccacc
      601 tacgcccact ggttttccta caccgccgcc gacttcatca gtgagaactg ggaattcgac
      661 caggcgattg ccaccaccga ccgcttgttg cctgagttcc ggcgccgtta cgtgggggat
      721 gctgggctgg atgaccagaa attgtttgcc gccctaagcc aggatgaatt tgtcatccgc
      781 gtgagccact tggaacgctg ggtggaggtc tataacgagc gcctggaccg ctgggagagg
      841 gaacagctgc aagatttagt cgctactgtg cccttcaccc atacctttag cgtcaccttt
      901 acccatgctc gcaaaaagcg ccgcacgaga gtcaaagtcg gctggctgac cttgcgcttg
      961 cctgacaccc accaacgctt atgggccgtg gttatctatg atcccccgct cgaccgcacc
     1021 ctggtcttat tgaccaacgt ccctttgttt accatcgcgg atgtccgcca agtatatgac
     1081 gactggcgtc tccgcccgcg cgttgagcat ggttaccgct ttgaccaaga gcaaggtctc
     1141 gatgtcgaag atatgcgcgt gcaatcactg gaggccatgc agcggctttt tgtgctcgtg
     1201 ttagcagccg cccaatttgt ctttttcctc atcaacacct ggcctcagca ggcagttttg
     1261 tgggtgcgtt ccctgggcgg caaactcggg ctggcgaatg acctggatgg cccttatctg
     1321 gtcttacgcg gcctcagtgc tttgattcaa gctctggtga ctctctcctt cctttcgatc
     1381 tcccctttcc cacatcagga cttcacttat gggtaatcac caaggagttt tcaaattcct
     1441 ttttgactct tgacgttttg gcttgtttat gtaaaataga cgtttatgga atttcaaagt
     1501 accgcaccgt tgattgttca aggcgatcgc acggtcc
//
LOCUS       DAOTLW010000136         1493 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_69023_length_1493_cov_6.725313, whole genome shotgun sequence.
ACCESSION   DAOTLW010000136 DAOTLW010000000
VERSION     DAOTLW010000136.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1493)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1493
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69023_length_1493_cov_6.725313"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(334..573)
                     /locus_tag="P5516_09265"
     CDS             complement(334..573)
                     /locus_tag="P5516_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92656.1"
                     /translation="MLPPLFITSSFTATTQPAGGAGFIAERGQAHQVKLVVFYIPFPS
                     TQALFELQRLHNLLGWSTRFPPLWKINTSIKALWQ"
     gene            572..1486
                     /locus_tag="P5516_09270"
     CDS             572..1486
                     /locus_tag="P5516_09270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92657.1"
                     /translation="MNEILDRLEKSVDPCVRYLFRRDYLRENPENPEMLALQEEIRHS
                     SRVRLMLERRDAQGRFPWHPSSKWVGAFWTLLMLADIGYPPGDQDLAPLRDQVLDWLL
                     SPQHLNKVPQINGRWRRCALQEVSIVYSSLKLGLANERIPQVVENLLQWQWPDGGWNC
                     DKKHAAVHSSFHETWIPLLAMHTYALASGSPRAQESSRRASEVFLQHRLFRRTNDGEV
                     MDANFGKIAYPPYWHYDILVGLRVMDVVGHLSDPRCGDALDWLASLRLPDGGGCRGTE
                     VLQGICREEGRLRGVICGLGQHRQRENE"
BASE COUNT          348 a          345 c          438 g          362 t
ORIGIN      
        1 acgaaaagag cctgtcggcg atgatggcgc ggcgcagcaa tttgccttcg ccgataatgt
       61 gatcctgacc aatgaattca tccagtgtgc gcgggcgcaa cctggctgcc agcggtgctt
      121 cgtgatgcag gttttcatta agcgcgtggt cgaataaatc catcgactta ttgtagcatg
      181 gaacatcagt gatagaacaa aatacccatt tcaattgcgg aaaaggccga atatctttaa
      241 aacgaaagcg gctcctttcg ccgggaggtc gctattgcca gacggtttta atgaattgcg
      301 gtgtgccatg tggaacgcgt ttccctcatt cgctcattgc caaagcgcct tgatcgatgt
      361 gttgattttc cacaatggag ggaaccgggt tgaccagcca agcaggttgt gcagtcgctg
      421 aagttcaaaa agcgcctgag tgctggggaa tgggatgtaa aaaacgacaa gctttacctg
      481 gtgggcttga ccgcgctcag cgataaatcc cgcgccgcct gcaggctggg tggttgcggt
      541 aaacgaactg gttataaata aaggaggaag catgaatgag attcttgacc gtctggaaaa
      601 atcagtagac ccctgtgtgc gttatctttt ccggcgcgat tatctgaggg aaaaccctga
      661 aaacccggaa atgctggctt tgcaggagga aatacgccac tccagccgtg tgcgcttaat
      721 gctcgaaagg cgagatgcgc agggacgctt cccgtggcat ccctcttcca aatgggttgg
      781 cgcgttttgg acgctgttga tgctcgctga cattggctac ccgccgggag accaggacct
      841 tgcaccgctg cgtgaccagg tgctggattg gttgttgagc ccgcagcatt tgaacaaagt
      901 cccgcagatt aatggccgct ggcggaggtg cgcgctgcaa gaggtttcta ttgtgtactc
      961 ttcgttaaaa ttgggtcttg cgaatgaaag aataccgcag gtagtggaga atttacttca
     1021 atggcaatgg ccggacggcg gctggaactg cgataaaaaa cacgccgcag ttcattcttc
     1081 ctttcatgaa acctggatac cgctgctggc tatgcacact tacgcgctgg cttctggttc
     1141 tccgcgcgcg caagaatcat cgcggcgcgc cagcgaagtt tttcttcaac acaggttatt
     1201 caggcgtaca aatgatggcg aggtgatgga tgcgaatttt ggtaaaatcg cgtatcctcc
     1261 atattggcat tatgatatcc ttgtcggtct gcgggtgatg gacgtggttg gtcatctttc
     1321 cgacccgcgc tgcggagacg cgctcgattg gctcgcttcc ttgaggttac cagatggggg
     1381 gggttgccgt ggaacagaag tattacaagg tatctgcaga gaagaaggaa ggctccgcgg
     1441 tgtcatttgt ggattggggc agcaccggca aagggaaaat gaatgagttt gct
//
LOCUS       DAOTLW010000137         1491 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_69157_length_1491_cov_4.886490, whole genome shotgun sequence.
ACCESSION   DAOTLW010000137 DAOTLW010000000
VERSION     DAOTLW010000137.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1491)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1491
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69157_length_1491_cov_4.886490"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            571..>1491
                     /locus_tag="P5516_09275"
     CDS             571..>1491
                     /locus_tag="P5516_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92658.1"
                     /translation="MNEILDHLEKSVDPCVRYLFRREYLRENSENPEMLALQEEIRHS
                     SRVRLMLARRDAQGRFPWHPSSKWVGAFWTLLMLVDIGYPPGDQGLAPLRDQVLDWLL
                     SPQHLNKVPQINDRWRRCALQEASIVYSSLKLGIENERIPQVVENLLQWQWPDGGWNC
                     DKKPAAVHSSFHETWIPLLAMHTYALASGSPRAQESSQRASEVFLKHRLFRRIKDGEV
                     MDTNFGKIAYPPYWHYDILVGLRVMDMVGRLSDPRCGDALDWLASLRLPDGGLAVEQK
                     YYKVSADKKEGSGVSFVDWGSTGKGKMNEFA"
BASE COUNT          359 a          348 c          432 g          352 t
ORIGIN      
        1 acgaaaagag cctgtcggcg atgatggcgc ggcgcagcaa tttgccttcg ccgattatgt
       61 gttcctgacc aacgaattca tccagtgtgc gcgggcgcaa cctggctgcc agcggcgctt
      121 cgtgctgcag gttctcatta agcgcgtggt caaatagatc catcgacata ttgtagcatg
      181 gaacatcaga aatagaacaa aatacccatt tctattgcgg aaaagaccga atatctttaa
      241 aatgagtgtg gctcctttcg ccggcaggct gctattgcca gacggtttta atgaattgcg
      301 gtgtgccatg aggaacgcgt tcccctcatt cacccattgc caatgcgcct tgatcgatgc
      361 gctgattttc tttagtggag gagatagggt tgaccagcca agcagcttgt gcagtcgttg
      421 aaatttaaaa agcgtcgaag tgataggaaa tgggatgcaa aaatgaatta gccttacctg
      481 gtgggcttga ccgcgctcag cgataaattc cgcgccgctg caggatgggt ggatgcgaca
      541 aatgagctgg tgataacaaa aggaggaagc atgaatgaga ttcttgacca tctggagaaa
      601 tcagtagacc cctgtgtgcg gtatcttttc cggcgcgaat acctgaggga aaactctgaa
      661 aacccggaaa tgctggcttt gcaggaggaa atacgccact ccagccgtgt gcgcttaatg
      721 ctcgcaaggc gagatgcgca gggacgcttc ccgtggcatc cctcttccaa atgggtgggc
      781 gcgttttgga cgctgttgat gctcgttgac attggctacc cgccaggaga ccagggtctt
      841 gcaccgctgc gcgaccaggt gctggattgg ttgttgagcc cgcagcattt gaacaaggtg
      901 ccgcagatca atgatcgctg gcggaggtgc gcgctgcaag aggcttctat tgtgtactct
      961 tcgttaaagc tgggtattga gaatgaaagg ataccgcagg tagtggagaa tttacttcaa
     1021 tggcaatggc cagacggcgg atggaactgc gataaaaaac ccgccgcggt acactcatcc
     1081 tttcatgaaa cctggattcc gctgttggcc atgcacacgt acgcgctggc ttccggttct
     1141 ccgcgcgcgc aagaatcatc gcagcgtgcc agcgaagttt ttctcaaaca caggttattc
     1201 aggcgcataa aagatggcga ggtgatggat acgaattttg gtaaaatcgc gtacccccca
     1261 tactggcatt atgatatcct cgtcggtctg cgggtgatgg atatggttgg ccgtctttcc
     1321 gacccgcgct gcggagacgc gctcgattgg ctcgcttcct tgagattacc agatggcggg
     1381 cttgccgtgg aacagaagta ttacaaagta tctgcagata agaaggaagg ctccggggtg
     1441 tcatttgtgg attggggcag caccggcaaa gggaaaatga atgagtttgc t
//
LOCUS       DAOTLW010000138         1488 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_69322_length_1488_cov_11.683182, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000138 DAOTLW010000000
VERSION     DAOTLW010000138.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1488)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1488
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_69322_length_1488_cov_11.683182"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="P5516_09280"
     CDS             complement(<1..634)
                     /locus_tag="P5516_09280"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HRT92659.1"
                     /translation="MRIKFDCSTLPAAPNLEFEQALWRQGFLHVVGLDEAGRGALAGP
                     VYAAAVILPLGSDGLAEALVGVRDSKQLTSQKRESLAPIIQARALAWSVGWADVAEID
                     QLGIAPASRLAFCRALEGLQLPAEHLLMDYFHLPEVELPQTALVKGDQRSLSIACASI
                     LAKTARDACMRAQEAQFPGYGFAQHKGYGTAGHRAALEALGPCPLHRRSFH"
     gene            complement(618..1352)
                     /locus_tag="P5516_09285"
     CDS             complement(618..1352)
                     /locus_tag="P5516_09285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HRT92660.1"
                     /translation="MDTPFLSLIIPAHNEASRLAQTLQQVERFIAQQSYTIEVLLVEN
                     ASSDATVEIAQSWQARLPQMRLLCLEQPGKGNAVRVGMLAAQGTYRFMADTDFSMPIE
                     EINRFIPYLEAGADIAIGSREAPGAKRIGEPYQRHLVGRVFNTLVRWLVLPGVQDTQC
                     GFKCFTAAAAETVFSKQTLAGWSFDVEILVIARELGLKVQEVPITWVYGAGSRVNVLR
                     DSLRMTRDLLYIRRQARRGVYHADQV"
     gene            complement(1370..1446)
                     /locus_tag="P5516_09290"
     tRNA            complement(1370..1446)
                     /locus_tag="P5516_09290"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1410..1412),aa:Pro,seq:tgg)
BASE COUNT          339 a          456 c          406 g          287 t
ORIGIN      
        1 aatgaaagct cctcctgtgc agcgggcaag ggccaagcgc ctccagcgca gcgcgatgcc
       61 ctgctgtgcc atagccttta tgctgtgcaa agccataccc ggggaactgc gcttcctgcg
      121 cacgcatgca agcatcccgt gcggtcttgg ctaaaatcga ggcgcaggca atcgaaagtg
      181 aacgctgatc ccccttcacc aaggcggttt ggggcagctc cacttcgggc agatggaagt
      241 aatccatcag gaggtgctcg gctggcagct gcaacccctc cagcgcgcgg caaaaggcta
      301 agcggctggc tggggcaatg cccagctggt cgatctctgc cacgtctgcc caacccacac
      361 tccaagcaag cgcccgcgcc tgaatgatcg gagccaggct ttcacgcttc tgtgaagtga
      421 gctgcttgga atcgcgcact cctaccagtg cctctgctaa accatcactt cctaatggga
      481 gaataacagc ggcggcatac acaggtcccg ccagagcacc gcgtcccgcc tcatccagtc
      541 ctactacgtg caggaagccc tggcgccaca acgcctgttc aaactccagg tttggcgccg
      601 ccggcagagt cgaacagtca aacttgatcc gcatggtaaa ctcctcggcg ggcttggcgc
      661 cgaatataga gcaaatcgcg cgtcatgcgc aaggaatcgc gcagcacgtt cacacggctg
      721 ccagcgccgt aaacccaagt tatcggcact tcttggactt tcaagccaag ttctcgggca
      781 attaccaaga tctccacgtc aaagctccag ccagctaggg tctgtttgga aaagactgtt
      841 tccgccgccg ctgcggtgaa gcatttgaat ccgcattggg tatcctgcac gccaggcagg
      901 accagccagc gcaccagggt gttgaacacc cgccctacaa ggtgccgttg ataaggttca
      961 ccgatgcgct tcgcaccggg ggcttcgcgt gaaccaatgg cgatatctgc gccagcttct
     1021 aagtaaggaa taaaacggtt gatttcctca atgggcatgg aaaagtccgt atcagccata
     1081 aagcggtagg tgccctgtgc cgctaacatc cccacgcgca cagcattccc cttacctggc
     1141 tgctccaagc agagtaaacg catctgcggc aggcgcgcct gccaactttg agcgatctcc
     1201 acggttgcat cgctgctcgc attttcaacc agcagcactt caatagtgta ggattgctgc
     1261 gcgatgaagc gctcaacttg ctgaagagtc tgtgcgaggc ggcttgcttc gttatgtgca
     1321 gggatgatga gggagagaaa tggtgtatcc acaagcttta agtaaacact ggtcggggtg
     1381 ggcggacttg aaccgccgac ctctgcgtcc caaacgcagc gcgctagccg actgcgctac
     1441 accccggcga taaaaagtat atcctgaagc gcacccaaac gtcaagca
//
LOCUS       DAOTLW010000139         1451 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_71897_length_1451_cov_10.603868, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000139 DAOTLW010000000
VERSION     DAOTLW010000139.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1451)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1451
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_71897_length_1451_cov_10.603868"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..584
                     /locus_tag="P5516_09295"
     CDS             <1..584
                     /locus_tag="P5516_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92661.1"
                     /translation="VAQIFTANSGVGPTMADGGALFNATAVTTAGGHANLGTTALSAN
                     AWDQTCRNVYRQPMLIKNTDLLRGTGPMLAVNPKFILVPRALQKTALELCSGALVRES
                     GYVYDNVLKGSAVPVVVPDWADETDWAAACDPRVVPAIFVGERFGLMPEIFVAGDELS
                     PAVFTNDEHRLKVRHYLAVWVNDFRPLYKNNVA"
     gene            603..797
                     /locus_tag="P5516_09300"
     CDS             603..797
                     /locus_tag="P5516_09300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92662.1"
                     /translation="MPKWKALLSSRKFWAALMGLIVMVIKAWKPDFPLDGETLAGIFS
                     VLVAYILGTALEDGLAGHKQ"
     gene            864..1073
                     /locus_tag="P5516_09305"
     CDS             864..1073
                     /locus_tag="P5516_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92663.1"
                     /translation="MDDERLKGIVEALAGSNVLSYALKEGGVLVLIDQAGRKRRYLPE
                     DYRHLLRKAHKTAKLIEGGTNEQSQ"
     gene            1057..>1451
                     /locus_tag="P5516_09310"
     CDS             1057..>1451
                     /locus_tag="P5516_09310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92664.1"
                     /translation="MSNLSEICSRLNAQFFDLQNLAIPTGSQEEAVREALAGLNAFLD
                     KDFTVAGLDGAVLTTLPSDHEAVLLFGAAAFALDFSLRSHLVHTTSSTVRSEETLRLW
                     CEHLNRQFDAGLDRLRLQALQANGALPYSP"
BASE COUNT          335 a          352 c          430 g          334 t
ORIGIN      
        1 ttgtggcgca gattttcacg gctaatagcg gcgtaggccc aaccatggct gatggcggcg
       61 cgcttttcaa cgccactgcc gtaacgacag ctggcgggca tgccaacctg gggaccaccg
      121 cgctcagtgc taatgcctgg gatcaaacct gccggaatgt ttaccgccag ccgatgctga
      181 tcaaaaacac tgatctccta cgcggcacag gaccaatgtt agcagtcaac cccaaattca
      241 tccttgtgcc gcgggcgctg caaaagactg cgctggagct atgcagcgga gcgctcgtgc
      301 gcgagtccgg ctacgtttac gataacgtgc tcaaaggcag cgccgtgcct gtggtagtac
      361 cggattgggc ggatgagact gactgggcgg cggcttgtga cccgcgcgtg gtgccggcga
      421 tttttgtggg cgaacgcttt gggcttatgc cggagatctt tgtggcgggg gatgaactct
      481 cgccggcagt tttcaccaat gatgaacatc gcctgaaggt gcgccattac ttagccgtgt
      541 gggtgaatga tttccgtcca ctctataaga ataacgttgc gtaatgatga aaggagcaca
      601 agatgccaaa gtggaaagct ttactgagtt cgcgtaagtt ttgggctgcg ctgatgggtt
      661 tgattgtgat ggtgatcaaa gcctggaagc cggatttccc cttggatgga gaaaccttgg
      721 ctgggatttt ctccgtgttg gtggcgtaca tcctcggtac cgcgctggaa gacggcttag
      781 ctggtcacaa acaataatcg tgatgagggc aggcaaaaac ctgccctcct tgcgccaaaa
      841 tcatagataa aggagtaacc acaatggatg acgaacgcct caaaggtatc gtagaagcat
      901 tagctggctc gaatgtactc agttatgcac tcaaggaagg cggtgtgctt gtcctgattg
      961 atcaagcggg caggaagcgg cgctatttac ctgaagacta ccggcacctg ctgcgtaaag
     1021 cgcataaaac cgcaaaattg attgaaggag gcacaaatga gcaatctcag tgaaatttgc
     1081 agtcggctga atgcgcagtt ttttgacctg caaaacttgg caatccctac tgggagccag
     1141 gaagaagccg tacgggaagc tttggctggg ttaaacgcat ttctggataa agactttaca
     1201 gtggcgggct tggatggggc ggtgctgaca accctaccgt cggatcacga agcagtttta
     1261 ctcttcggag cggcagcttt tgcgctcgat ttcagcctgc ggagtcattt agttcacacc
     1321 accagcagca ccgtgaggtc ggaggaaacc ttacgcttgt ggtgtgagca tttgaaccgg
     1381 cagtttgatg ctggcttgga tcgcctgcgt ctgcaagcgt tgcaagcgaa tggtgcactg
     1441 ccttatagcc c
//
LOCUS       DAOTLW010000140         1417 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_74546_length_1417_cov_10.639501, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000140 DAOTLW010000000
VERSION     DAOTLW010000140.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1417)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1417
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74546_length_1417_cov_10.639501"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..652
                     /locus_tag="P5516_09315"
     CDS             <1..652
                     /locus_tag="P5516_09315"
                     /inference="COORDINATES: protein motif:HMM:NF040439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GWxTD domain-containing protein"
                     /protein_id="HRT92665.1"
                     /translation="PVDSCSLTQKFSLAADSIHIAGISGARVFNSPPGSYTADLTEQA
                     NADSLSGLRLSIYEGNICYEMEPFLYNQWFYYNARYSYKDQIQQLRYIATQNEWKSLR
                     AVPDEKMHEVIDKYWQVHDPSPGTLRNEARESFYKRVITADERFTIHKRLQGWKSDRG
                     RIYIKYGEPDEVHSEVHPLDLHPYIVWVYYNQNLEFIFADTGGFGQYKLRNKDEEY"
     gene            639..>1417
                     /locus_tag="P5516_09320"
     CDS             639..>1417
                     /locus_tag="P5516_09320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92666.1"
                     /translation="MKNTKALLLCLALASLAGTAAADSFTMHVSPQRFLDKDKNTIIN
                     VDYQIPYGNLWFLAQKGGYFAELNLQVEMVVGDQVIYSQTFRDNVGISDKNDARSNKS
                     YLNRLTFSVESEPYLLRLNAKDINSRKTATWTYQVAPLGEQDLLSDLELCSYAKPDSS
                     SFLERFHRGGILYQTQPSLIFDKTESEDLTIYFETYKPEDLADETGLLVLTVSKDSVM
                     VFDQFLDYIPTSSIEGVSLRIPLEKLNPGKYTGSVEFQLGE"
BASE COUNT          382 a          386 c          342 g          307 t
ORIGIN      
        1 cccggtggat agctgttcgc tcacccaaaa attcagcctc gccgcggata gcatccacat
       61 cgcgggtatt tctggcgcgc gcgtattcaa ttcaccgcca ggctcttaca cagccgacct
      121 gaccgaacag gccaatgccg attctctgtc cggcttacgc cttagcatct acgagggcaa
      181 catctgctac gaaatggagc ctttcctcta caaccagtgg ttttattata acgcgcgcta
      241 cagctataaa gaccagatcc agcaactgcg ctatatcgcc acacaaaatg aatggaaaag
      301 cctgcgggcc gttccggatg agaaaatgca cgaagttata gacaaatact ggcaggtaca
      361 cgatccgagc ccgggaaccc tgcgcaacga agcacgcgaa tccttttaca agcgcgtgat
      421 cacagccgac gaacgcttca ccatccataa aaggctgcag ggttggaaga gcgacagagg
      481 tagaatctac atcaaatacg gcgaaccgga tgaggtgcac agcgaagtgc atcccctgga
      541 cctgcatcca tatattgtct gggtctatta taaccagaat ctggagttta tcttcgccga
      601 caccggtggt tttggccaat acaaactaag gaacaaagat gaagaatact aaagcattac
      661 tgctctgtct ggccttggcc agcctggcag gaacggccgc ggctgactct ttcaccatgc
      721 atgtgtcccc gcagcgcttt ttggacaagg ataaaaacac cattatcaac gtcgattacc
      781 agatccctta cggaaatctt tggttccttg cccaaaaagg cggttatttt gctgagttga
      841 acctgcaggt tgagatggtg gttggcgatc aagttatcta tagccaaacc ttccgcgaca
      901 atgttggaat ctcggataaa aacgacgccc gctccaacaa aagctatctc aaccggttga
      961 ccttcagcgt ggaaagcgag ccctaccttc tgcgcctgaa cgcgaaagac atcaattcaa
     1021 gaaaaaccgc cacctggacc taccaggtcg ctccactggg cgaacaggat ctgctcagcg
     1081 acttggaatt atgcagttac gcgaagcctg acagcagcag ttttctggag cggtttcacc
     1141 gtggcggaat cctctaccag acccagccat cgctgatctt cgataaaacg gagagcgaag
     1201 acctcaccat ctattttgag acttacaagc cggaagacct ggctgacgaa accgggctgc
     1261 tggttctgac cgtatcaaaa gacagcgtga tggtgtttga ccagtttctg gactacatac
     1321 cgacctccag catcgaagga gtttccctgc gcatccctct ggaaaagttg aacccaggca
     1381 aatacacggg atcggtggaa tttcaacttg gggagtt
//
LOCUS       DAOTLW010000141         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_74772_length_1414_cov_8.063282, whole genome shotgun sequence.
ACCESSION   DAOTLW010000141 DAOTLW010000000
VERSION     DAOTLW010000141.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74772_length_1414_cov_8.063282"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="P5516_09325"
     CDS             complement(<1..871)
                     /locus_tag="P5516_09325"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRT92667.1"
                     /translation="MNVTALVFFVCAGIAALVYQRLKPHWRMPWLLVISAGFVVTWSW
                     QFVLVLLVYSALNYALGQLADPAKTHARAWARAGILINILFLFVFKYSDFYLPQFSAL
                     LSSLGALQPGSVLHLLLPVGLSFLMVQNISYLLDVVNQRLPAEKDFLRFCVYIFYFPK
                     LLSGPVERARSFLPKLATPLKLDRSLLERSAALIFTGLLRKLALANPLFNLIPAQAFV
                     TPLDYPGQNLVFWLLAYTFALYNDFAGYTAIVRGVSLWFGIELSANFNLPYYARNFTE
                     FWNRWHITLSNWLR"
     gene            complement(868..>1414)
                     /locus_tag="P5516_09330"
     CDS             complement(868..>1414)
                     /locus_tag="P5516_09330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92668.1"
                     /translation="LLFFRNWSRSDFLDSSLMLARRFADTTASGYERDANVGKGIEIP
                     PDPADPDEQENFKLFSNFSIDPGRLENLKSILDLHQDGTLVVIAEMPVHANYFTYFGN
                     EQAHANYLKELVPYVEENGGIFLPPPEWQRFPLYSRVDHHHLNAIGAVLHSRMLAQNL
                     AVLCLDQSTCLLPAADGMASP"
BASE COUNT          388 a          370 c          399 g          257 t
ORIGIN      
        1 ccctcagcca gtttgaaagg gtgatgtgcc agcggttcca gaattcggtg aaattccgcg
       61 cgtagtaagg caaattaaaa ttggccgaga gctcgatgcc aaaccacagg ctgacaccac
      121 gcacgattgc cgtatacccg gcgaaatcat tgtaaagggc gaaggtgtac gccagcagcc
      181 agaaaaccag attctgtcca gggtaatcca gcggggtaac aaatgcctgg gcagggatga
      241 ggttgaaaag cgggttggcc agcgccagtt tgcgcagcaa accggtgaaa ataagcgcgg
      301 cactgcgctc cagcagtgaa cggtcaagct tcagcggtgt ggcgagtttg ggtaaaaagg
      361 agcgggcgcg ctccaccggg cctgaaagca attttggaaa atagaagata tagacgcaaa
      421 aacgcagaaa atccttttcc gcgggcagac gctggttgac cacatccagc aggtaggaaa
      481 tgttttgcac catcagaaaa gacagaccca ccggcagcaa aaggtgcaac acgcttccgg
      541 gctggagcgc tcccagcgaa ctgagcaggg cagagaactg cggcaggtaa aaatcactat
      601 acttgaatac aaacaggaac aggatattga tcaggatacc ggcgcgcgcc caggctctgg
      661 catgggtttt cgcgggatca gcaagctgac ctaaggcata gttcagcgcg ctgtacacca
      721 gcagcaccaa cacgaactgc catgaccagg taaccacaaa gcccgccgaa atgaccagca
      781 gccacggcat acgccagtga ggcttcaggc gctggtaaac cagcgcagca atacccgcgc
      841 aaacgaagaa gaccagcgcg gtcacgttca tggcgacgcc atcccatccg ccgccggcag
      901 caggcaggtg ctttgatcaa ggcaaagaac agccaggttt tgagccagca tgcgcgagtg
      961 cagtaccgcg ccaatggcat tcagatgatg gtgatcaacc cggctgtaga gcggaaaacg
     1021 ctgccattca ggcggcggca gaaagattcc gccattctct tcaacgtacg gcaccagttc
     1081 tttcaggtaa tttgcatgcg cctgttcatt cccaaaatag gtaaagtaat tggcgtgcac
     1141 cggcatttca gcaatgacca ccagggttcc atcttgatgt aaatcaagga tgctttttag
     1201 gttctccaac ctacccggat caatggaaaa attcgagaag agtttaaagt tttcctgttc
     1261 atcggggtcg gccgggtcag gcggaatttc gatgcccttg cccacattgg catcacgttc
     1321 ataacccgag gctgtagtgt ctgcaaagcg gcgcgccagc atcagggagg agtcgaggaa
     1381 atcactccgg ctccagtttc ggaagaagag cagg
//
LOCUS       DAOTLW010000142         1414 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_74775_length_1414_cov_6.749080, whole genome shotgun sequence.
ACCESSION   DAOTLW010000142 DAOTLW010000000
VERSION     DAOTLW010000142.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1414)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1414
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74775_length_1414_cov_6.749080"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..871)
                     /locus_tag="P5516_09335"
     CDS             complement(<1..871)
                     /locus_tag="P5516_09335"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HRT92669.1"
                     /translation="MNVTALVFFVCAGIAALVYQRLKPHWRMPWLLVISAGFVVTWSW
                     QFVLVLLVYSALNYALGQLADPAKTHARAWARAGILINILFLFVFKYSDFYLPQFSAL
                     LSSLGALQPGSVLHLLLPVGLSFLMVQNISYLLDVVNQRLPAEKDFLRFCVYIFYFPK
                     LLSGPVERARAFLPKLTTPLKVDRSLLERSATLIFTGLLRKLALANPLFNFVPAQAFV
                     TPLDYPGQNLVFWLLAYTFALYNDFAGYTAIVRGVSLWFGIELSANFNLPYYARNFTE
                     FWNRWHITLSNWLR"
     gene            complement(868..>1414)
                     /locus_tag="P5516_09340"
     CDS             complement(868..>1414)
                     /locus_tag="P5516_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92670.1"
                     /translation="LLFFRNWSRSDFLDSSLMLARRFADTSTSGYERDANVGKSIEIP
                     PDPADPDEQENFKLFTNFSIDPGRLENLKSILDLHEDGTLVVITEMPVHANYFTYFGN
                     EQAHENYLKELVPFVEKNGGIFLPPPAWQRFPLYSRVDHHHLNAIGAVLHSRMLAQNL
                     AVLCLDQSTCLLPAADGMASP"
BASE COUNT          386 a          365 c          400 g          263 t
ORIGIN      
        1 ccctcagcca gtttgaaagg gtgatgtgcc agcggttcca gaattcggtg aaatttcgcg
       61 cgtagtaagg caaattaaaa ttcgccgaga gctcgatacc aaaccacagg ctgacgccgc
      121 gcacgattgc cgtatacccg gcgaaatcgt tgtaaagggc gaaggtgtac gccagcagcc
      181 agaaaaccag attctgtccc gggtaatcca gcggggtaac aaacgcctgg gcagggacga
      241 agttgaaaag cgggttggcc agcgccagtt tgcgcagcaa accagtgaaa ataagcgtgg
      301 cactgcgctc cagcagtgaa cggtcaacct tcagcggtgt ggtgagtttg ggtaaaaagg
      361 cgcgggcgcg ctccaccggg cctgaaagca atttaggaaa atagaagata tagacgcaaa
      421 aacgcagaaa atccttttcc gcgggcagcc gctggttgac cacatccaga aggtaggaaa
      481 tgttttgcac catcagaaaa gatagcccca ccggcagcaa gaggtgcaac acgcttccgg
      541 gctggagcgc tcccagcgaa ctgagcaggg cagagaactg cggcaggtaa aaatcactat
      601 acttgaatac aaacaggaac aggatattga tcaggatacc ggcgcgcgcc caggctctgg
      661 catgggtttt cgcgggatca gcaagctgac ctaaggcata gttcagcgcg ctgtacacca
      721 gcagcaccaa cacgaactgc catgaccagg taaccacaaa gcccgccgaa atgaccagca
      781 gccacggcat acgccagtga ggcttcaggc gctggtaaac cagcgcagca atacccgcgc
      841 aaacgaagaa gaccagcgcg gtcacgttca tggcgacgcc atcccatccg ccgccggcag
      901 caggcaggtg ctttgatcaa ggcaaagaac agccaggttt tgagccagca tgcgcgagtg
      961 cagtaccgcg ccaatggcat tcaggtggtg gtgatcaacc cggctgtaga gcggaaaacg
     1021 ctgccaagcg ggtggcggca gaaagattcc gccattcttt tcaacgaacg gtaccagttc
     1081 tttcagataa ttttcatgcg cctgttcatt cccaaaataa gtgaagtaat tggcgtgcac
     1141 tggcatttca gtaatgacga ccagggttcc atcttcatgc aaatcaagga tgctttttag
     1201 gttctccaac ctacctgggt caatggaaaa attcgtaaag agtttgaagt tttcctgttc
     1261 atcggggtcg gccgggtcag gcggaatttc gatgctcttg cctacattgg catcacgttc
     1321 ataacccgag gttgaagtgt cggcaaaacg gcgcgccaac atcagggagg agtcgaggaa
     1381 atcactccgg ctccagtttc ggaagaagag cagg
//
LOCUS       DAOTLW010000143         1412 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_74948_length_1412_cov_12.302874, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000143 DAOTLW010000000
VERSION     DAOTLW010000143.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1412)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1412
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_74948_length_1412_cov_12.302874"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /locus_tag="P5516_09345"
     CDS             complement(<1..478)
                     /locus_tag="P5516_09345"
                     /inference="COORDINATES: protein motif:HMM:NF016155.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid biosynthesis protein"
                     /protein_id="HRT92671.1"
                     /translation="MPETVFLIFELLTLLLFAACLWHAARQGKTRVLELLASLVYGVS
                     LEWMTLRQLEAYHYGQFLVMIDGAPLCIGLGWAVILYSSMEFVKQLDMPLFAQPFLVG
                     LLALNIDAAMDAVAIRLGFWNWVIPLDMQWFGVPYGNFWAWFIVATSFSGMVYWLRA"
     gene            646..>1412
                     /locus_tag="P5516_09350"
     CDS             646..>1412
                     /locus_tag="P5516_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92672.1"
                     /translation="MAITDLYHAIEAACFALSPILKPVVEKQSSAFGLTAQEMVLLFV
                     APIFEPECVSASLLNRRSPYTSPDLYRKALENLANGGMLSSRDGVGFRVTEQGLETIR
                     KLNQSLQKTLAGIQPLPITRMMDLASRLKDLADAYLETPEPPGNWCIKHARRLDPGRK
                     APMMARVNQFLEELIAFRDDAHLAAWQGYEENGHAREILTYLWVEKQAVLGIIHQSLS
                     YRGFSLEDTLSAASELIRKGWVSREGDLLRITPFGRE"
BASE COUNT          373 a          378 c          365 g          296 t
ORIGIN      
        1 tcgctcgcag ccagtaaacc atcccgctga aagaagtagc cacaatgaac caggcccaga
       61 agttcccgta aggcacgcca aaccactgca tgtccagcgg gatcacccag ttccagaaac
      121 ccaggcggat ggcgaccgcg tccatggcgg cgtcgatatt caatgccagc aggcctacca
      181 gaaaaggctg ggcgaacagc ggcatgtcca gctgcttgac gaactccatg ctgctgtaaa
      241 gaatgactgc ccaacccagc ccaatacata acggcgcgcc gtcgatcatc accaggaatt
      301 gaccataatg ataggcttcc agctgcctga gggtcatcca ctccaggctt acgccgtaca
      361 ccaggctggc gagtaactcg agtaccctgg tcttcccctg ccgcgcggcg tgccaaagac
      421 aggcggcaaa caacagcagg gtcagcaatt caaaaatgag aaatacagtt tccggcattt
      481 acttcatccc caattcagtg tgtttacatt cgattcattc aaggagattc tattgtatct
      541 gaaaacgaga taaaaaacct gacttgtcac ttgaattacc ttcacctttg tgaaataata
      601 cttctatact gcaggctgga aaagcaggac agacagggag ggaaattggc gataacggac
      661 ctgtatcacg ccatagaagc tgcttgtttt gcgctgtctc caatcctcaa accagtggtt
      721 gaaaagcaat ccagcgcctt cgggttgaca gcgcaagaaa tggtacttct cttcgtcgcc
      781 ccaatctttg agccagagtg cgtcagcgcg agtttgctga ataggcgctc cccttatact
      841 tctcctgacc tgtaccgcaa agcgttggaa aacctggcaa acggggggat gttatcctca
      901 agggatgggg ttggcttccg cgttacagaa cagggtcttg aaacgatcag gaagctcaac
      961 caatctctcc agaagaccct ggcgggaata caaccccttc cgataacccg tatgatggac
     1021 ctggcaagcc gcttaaaaga cctggcggat gcctacctgg aaacgcctga accaccggga
     1081 aactggtgca ttaagcacgc acgccgtttg gaccctggca ggaaagcgcc gatgatggcg
     1141 cgtgtgaacc agtttttgga ggagttgatc gcgtttcgcg atgacgcgca cctggctgcc
     1201 tggcaggggt atgaagaaaa tggtcacgct agagaaattc tcacctatct atgggtagaa
     1261 aaacaggcgg tattgggaat cattcatcag tccctcagct accgaggttt ctcgctggaa
     1321 gatacgctga gcgctgccag cgaattaatc cgtaaaggct gggtgagccg cgaaggagat
     1381 ttgctgcgca tcacgccttt tggtagagaa at
//
LOCUS       DAOTLW010000144         1408 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_75277_length_1408_cov_11.067258, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000144 DAOTLW010000000
VERSION     DAOTLW010000144.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1408)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1408
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_75277_length_1408_cov_11.067258"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..1293
                     /locus_tag="P5516_09355"
     CDS             <1..1293
                     /locus_tag="P5516_09355"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HRT92673.1"
                     /translation="GKRAEAYEKNRHFSFNPDELDAVLLSHAHIDHSGNLPNLAKIGY
                     TGPIYATYPTATLGEIMLMDSAHIHEYDAEYINKKRARHGEPPIEPLYNRADAARAIE
                     LYYPVHYNEPFSPVPGVTARFIDAGHILGSAAIRLDIEENGTHKSLWFSGDIGRFGLP
                     LITDPVMPSDVDYLLMECTYGDMEHFTVEEAYQKFSQAVVKTIDRGGRVIVPAFAVGR
                     TQEIVYFLNQMSTQGLISRLPVYVDSPLAVQASQIFASYPEYFDEETNEFIRTQKHQA
                     LNFDGLTYISSVEESKALNDKKGPMVIISASGMAETGRILHHLKNNIGNPRNTVLIVG
                     WQAPDTLGRRLMEGADQLRIFGQLYDRRAEVQVITGLSAHAGQHLLLDYAGAVKERVK
                     EIFLVHGEEDAAGVFMGKLKEKGFAHFRYPSPGDTAEL"
     gene            1320..1407
                     /locus_tag="P5516_09360"
     tRNA            1320..1407
                     /locus_tag="P5516_09360"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1354..1356,aa:Ser,seq:cga)
BASE COUNT          331 a          391 c          382 g          304 t
ORIGIN      
        1 ggcaaacggg ctgaagccta tgaaaaaaac cgccatttca gtttcaaccc cgatgaactg
       61 gacgcagtat tgctttctca cgcgcacatt gatcatagtg gcaacctgcc caacctggcc
      121 aagatcggtt ataccggtcc gatctacgcc acttacccca ccgccaccct cggcgagatc
      181 atgttgatgg attcggcgca catccacgaa tatgacgctg aatatatcaa caaaaaacgc
      241 gcccgccatg gcgaaccacc cattgaacct ctctacaacc gggcagacgc cgccagggct
      301 attgagctgt attacccggt tcattacaac gagccctttt caccggtgcc gggtgtaacc
      361 gccaggttca tcgacgccgg tcatatcctc ggttcagccg ccatccgcct ggatatcgaa
      421 gaaaacggta cccacaagag cctgtggttt tcaggtgaca ttgggcgctt tgggctgccc
      481 ctgatcactg atccggtcat gcccagcgat gtggattacc tgttgatgga atgcacctat
      541 ggcgacatgg aacacttcac cgtggaagag gcttaccaaa aattcagcca ggctgtggta
      601 aagaccattg accgtggcgg cagggtgatt gtcccggctt ttgccgtcgg gcgcacacag
      661 gagatcgttt acttcctcaa ccagatgagc acgcagggtt tgatctcgcg gctgcccgta
      721 tatgtagaca gccccctggc ggtgcaggct tcacagatct ttgccagtta cccggaatac
      781 ttcgacgagg aaaccaacga attcatccgc acgcaaaaac accaggcgct caatttcgac
      841 gggctcacgt atataagttc agtagaagag tcaaaagccc tgaacgataa aaaagggcca
      901 atggtcatta tctcggcttc tggtatggcc gaaaccgggc gcatcctgca tcatttaaag
      961 aacaacattg gcaacccgcg caatacagtc ctcatcgttg gctggcaggc gccagacaca
     1021 ctgggcaggc gcctgatgga aggcgctgat caactgcgca tcttcggtca gctttacgat
     1081 cggcgtgccg aagtgcaggt catcaccggt ctctcggcgc acgccggtca gcacctgctg
     1141 ttggattacg ccggtgcggt aaaagagagg gtaaaagaga tttttttggt tcacggtgaa
     1201 gaggatgccg ctggggtgtt catggggaag ttaaaagaaa aggggtttgc gcatttccgt
     1261 tacccctcac caggcgacac cgccgagttg tgagagattt cgggtataat tctggcgctg
     1321 gagaggtgcc ggagtggttg atcggggcgg tctcgaaaac cgttgtggct ctcgtggtca
     1381 ccgtgggttc gaatcccacc ctctccgc
//
LOCUS       DAOTLW010000145         1397 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_76162_length_1397_cov_20.140835, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000145 DAOTLW010000000
VERSION     DAOTLW010000145.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1397)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1397
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_76162_length_1397_cov_20.140835"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1373)
                     /gene="dnaK"
                     /locus_tag="P5516_09365"
     CDS             complement(<1..1373)
                     /gene="dnaK"
                     /locus_tag="P5516_09365"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HRT92674.1"
                     /translation="MLPSVVAFNKNGERLVGIPAKRQAIVNPENTVYSIKRFMGRRYD
                     EVETERKMVSYKVVPGPSGDVRVEIPVTGQTYTPQEISAMILGKLKSDAEAYLGEKVT
                     QAVITVPAYFNDSQRQATKDAGKIAGLEVLRIINEPTASALAYGLDKRENEIILVFDL
                     GGGTFDVSLLEVGGGVVEVKATNGDTHLGGDDWDEVIVNWAADEFKKEQGIDLRKDRQ
                     ALQRLREAAEKAKIELSSVMETEINLPYITADANGPKHLLLKLTRAKFEQMTEHLLER
                     CKIPFNAVLKDANLTIDKINEVVLVGGATRMPMVQDLVRKLTNGKEPNKGVNPDEVVS
                     VGAAIQGGVLSGEVKDVLLLDVTPLSLGVETLGGVMTKLIERNTTIPVKKTEVFSTAE
                     DNQTSVDIHVLQGERPLAADNMTLGRFRLDGIPPAPRGIPQIEVTFDIDANGILSVTA
                     KDKATGK"
BASE COUNT          305 a          396 c          330 g          366 t
ORIGIN      
        1 tctttgcccg tggctttatc cttagcggtc acgctcagga tgccgttggc atcgatatca
       61 aaggttactt caatctgcgg aataccacgc ggcgccggcg gaattccatc taagcggaag
      121 cggcctaagg tcatgttatc cgcagccagc gggcgctcgc cctgcagcac gtggatatcg
      181 accgaggtct gattatcttc tgctgtggag aacacttccg tctttttcac cggaatggtg
      241 gtgttgcgct cgatcagctt ggtcatcacg ccgcctaagg tttctacacc taaggagagc
      301 ggggtcacgt ccaggagcaa aacgtctttc acttcgcccg agagcacgcc accctgaatg
      361 gctgcaccaa cagacaccac ctcatctggg ttcacgccct tattaggctc tttgccgttg
      421 gttagcttac gcaccaaatc ttgcaccata ggcatgcggg tggcaccacc aaccaatacg
      481 acctcattga tcttatcaat tgtcaggttg gcatctttca acactgcgtt gaatggaatc
      541 ttgcagcgtt ccagcaggtg ctctgtcatt tgctcaaatt tcgcccgcgt cagcttgagc
      601 agcaagtgct ttggaccatt ggcgtctgcg gtaatataag gcaggttgat ttcagtctcc
      661 atcacggagc ttaattcgat tttcgccttt tcagcagctt cgcgtaagcg ttgcaatgcc
      721 tggcgatcct tgcgcaggtc gatgccttgt tctttcttga actcatccgc agcccagttg
      781 acgatgactt catcccagtc atcaccacca aggtgggtat cgccattggt ggctttcact
      841 tccacaacgc cgccgcccac ttccagcaag ctcacgtcaa aagtaccgcc acccaagtca
      901 aagaccaaga tgatttcgtt ctcacgttta tccaagccat aagccaaagc ggaagccgtc
      961 ggctcgttga taatgcgcaa cacttccagc ccggcaatct tgcctgcatc tttggtcgct
     1021 tggcgctggc tatcgttgaa gtaagccggc acagtgatta ccgcttgagt aaccttttcg
     1081 cctaaatatg cctctgcatc gctcttgagt ttgcccaaga tcatggcaga tatttcctgc
     1141 ggcgtataag tctggcctgt gacgggaatc tccacgcgga catctccgct tggcccaggg
     1201 accactttgt acgaaaccat tttgcgttcg gtttcgactt catcatagcg ccgtcccata
     1261 aagcgcttaa ttgaataaac agtattttcc gggttgacaa ttgcctgccg ttttgcagga
     1321 atgccgacaa gccgttcacc atttttatta aaagctacaa ctgaaggcag caaatgcgaa
     1381 ccttctgagg ttggaat
//
LOCUS       DAOTLW010000146         1385 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_77157_length_1385_cov_8.782707, whole genome shotgun sequence.
ACCESSION   DAOTLW010000146 DAOTLW010000000
VERSION     DAOTLW010000146.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1385)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1385
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77157_length_1385_cov_8.782707"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..410
                     /locus_tag="P5516_09370"
     CDS             <1..410
                     /locus_tag="P5516_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92675.1"
                     /translation="FTDEPVNQNLPYAIEGEPAAMANTVFHPGEGCDWQGVAGRVVDL
                     QGRPMVGVVVKLTGLYGGRTIDMTTLSGGAAAWYGASGYEFILGDKPISSNAMLAVQL
                     VDQALMPLSNRVIFHTYETCDKNLILINFKQVR"
     gene            complement(448..>1385)
                     /locus_tag="P5516_09375"
     CDS             complement(448..>1385)
                     /locus_tag="P5516_09375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92676.1"
                     /translation="RDFSPVNTQRIARDSIRELLSETGEPTAYIKLHTATLCALAQEN
                     AFPPTIQQLTVEKAAEYQSTLNSLLADPGFLVRLDATAQDPESGLWWLAVPEGTAVPL
                     ADRLEIEIAGWLQKAGHLTLAALLERVYQEFPGYLAPPEELVRQCLASYADSDATGEV
                     WTLKPHDQVDCRLDDIEEMRKLLTTLSDRFHLLQVDNSVITWHQGDPAELPIYRFFIS
                     SSAVIDRQSLLLIEPAPCQTIYVLPGSRAGLLKFKSERDPYLRQLLGPRIHFLKFRTL
                     RSIASRADLSLELFKVLVDSDPLSLEETTQLSMFR"
BASE COUNT          319 a          335 c          415 g          316 t
ORIGIN      
        1 tattcaccga cgagccggtg aaccaaaacc tgccatacgc catcgaggga gaaccggcgg
       61 ctatggccaa cacggtcttt cacccgggcg agggatgtga ctggcaaggt gtggccggga
      121 gggtggtgga cctgcaaggc agacctatgg ttggggtggt agtgaagttg accggcctgt
      181 atggtggccg aacaattgat atgaccacgc tctctggagg ggcggcggcg tggtatgggg
      241 catcgggcta cgaattcatt ttgggcgaca aaccgatcag ttcaaatgcg atgctggctg
      301 tgcagttggt ggaccaggca ttgatgccgc tttcaaaccg ggtcatcttt cacacctacg
      361 agacctgtga taaaaacctg attctcatta atttcaagca ggttcgctga atatccactg
      421 atccattgac cgatgggccg ttttttctta acggaacatc gaaagttggg tggtttcttc
      481 caggctgagc ggatctgaat cgaccagcac tttgaatagc tccaaagaca gatcagcgcg
      541 gctggcgatg ctgcgcaggg tgcggaactt gaggaagtga atccgtggac ccaagagctg
      601 acgcagataa ggatcgcgtt cgctcttgaa cttcaacaaa cccgcgcggc taccaggcag
      661 cacataaatg gtctggcagg gtgccggttc aattagaaga agcgattgac gatcgatcac
      721 cgcagaagat gagatgaaaa aacgatagat gggtaactcc gcggggtcac cttggtgcca
      781 ggtaatgacc gaattgtcca cttgcagcag gtgaaaccgg tcagagagag tggtaagcaa
      841 tttacgcatt tcctcgatat cgtcaagccg gcagtcaacc tggtcgtgag gtttcagcgt
      901 ccatacctct ccagttgcgt ctgaatccgc gtaagacgcg aggcactgac gcaccagctc
      961 ttctggagga gcaaggtaac cagggaattc ttggtaaacg cgttcaagca gcgctgccag
     1021 ggtgagatgg cctgcttttt gcagccaacc ggcaatttcg atttccagtc tgtctgctaa
     1081 aggaacagct gttccttccg ggacagccag ccaccataat ccactctctg ggtcctgggc
     1141 ggtggcatcc agacgcacca aaaagcctgg atctgccagc aatgaattga gggtggattg
     1201 gtactcagca gctttttcta cggtcagctg ttgaatggtt ggcgggaagg cgttttcttg
     1261 cgccaatgcg cacagggttg cggtgtgaag cttgatgtag gcggtgggtt ctcctgtttc
     1321 gctgagcagt tcgcggatgg aatctcgggc gatcctctgt gtgtttacgg gtgaaaaatc
     1381 cctcc
//
LOCUS       DAOTLW010000147         1384 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_77243_length_1384_cov_8.489842, whole genome shotgun sequence.
ACCESSION   DAOTLW010000147 DAOTLW010000000
VERSION     DAOTLW010000147.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1384)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1384
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77243_length_1384_cov_8.489842"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..440
                     /locus_tag="P5516_09380"
     CDS             <1..440
                     /locus_tag="P5516_09380"
                     /inference="COORDINATES: protein motif:HMM:NF025011.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-associated domain-containing protein"
                     /protein_id="HRT92677.1"
                     /translation="TLTNHTPRIARYQFARFSEWLEVSPKRYRFRITPTSLQTAASQG
                     LKVRHLIALLRKYAGVNLPPSLVAALQRWEAHGREAALTQAWVLKLSAPEVLQALRAS
                     PAAGSLGEALSPVAVIVKPDGIEKVRMALARLGYLSDLENLPQ"
     gene            437..859
                     /locus_tag="P5516_09385"
     CDS             437..859
                     /locus_tag="P5516_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92678.1"
                     /translation="MKRKPHGFLGWMVLLLLLWNAGSGILRVVGAWQQRALLPQIGLP
                     GWLPGYLLVVGALTALLNLGAFVCLYTRRRQAITLAWVALVVTLAGYWLERLLLWAPD
                     QRSGNVVFMAVFHTLCLAALGGYTYHESKERGEDGSGN"
     gene            843..>1384
                     /gene="cyaB"
                     /locus_tag="P5516_09390"
     CDS             843..>1384
                     /gene="cyaB"
                     /locus_tag="P5516_09390"
                     /inference="COORDINATES: protein motif:HMM:TIGR00318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class IV adenylate cyclase"
                     /protein_id="HRT92679.1"
                     /translation="MDQEIEVKFYLQDLAAFRQRLLSAGAFLTQPRVKEINLRFDTPQ
                     RDLSQKAQVLRLRQDEAAHLTFKGPGALEGGVLSRQEIEFEVSDFAAARRFLEALGYR
                     VFTTYEKIRENFTLAGVQVSLDEMPFGNFTELEGPNPDAIRKLAGSLGLAWERRIAMS
                     YLELFARLKARLALPFDDLT"
BASE COUNT          265 a          384 c          423 g          312 t
ORIGIN      
        1 tgacgctcac caaccacacc ccgcgcatcg cgcgttacca attcgcccgc ttttcagaat
       61 ggctggaagt gagcccgaag cggtaccgct tcagaattac gcccacatcg ctgcaaacgg
      121 cggcgagtca agggctgaag gtgcggcacc tgatcgctct gctgcgtaaa tatgccggtg
      181 taaacctgcc cccgagcttg gtggcagcct tgcaacgctg ggaggcacac gggcgcgagg
      241 cagcgctcac acaagcttgg gttctgaagc tcagcgcgcc ggaggtgctg caagccctgc
      301 gggcttcgcc ggctgcaggc agcttaggcg aagcgctaag cccagtagcg gtcatcgtaa
      361 agcctgatgg tattgagaaa gtgcgcatgg cattggcacg cttaggttac ctcagcgacc
      421 tggaaaactt gccgcaatga agcgtaagcc gcacggattt ttaggctgga tggtgctcct
      481 gctcttgctc tggaacgctg gcagcggcat tttacgcgtg gtaggcgcct ggcagcagcg
      541 cgcgctgctc ccgcagatcg ggctgcccgg ttggctgccc ggatatctac tggtggtggg
      601 tgctctcaca gccctgttga acttaggcgc cttcgtttgc ctatataccc gtcgtaggca
      661 agctataacc ttggcttggg ttgcgctggt tgttacatta gctggctact ggctggagcg
      721 cctgctgctt tgggcgccag atcagcgcag cggcaacgtg gtttttatgg cagtctttca
      781 cacgctctgc ctggcagcct taggcggtta tacttatcat gaaagcaaag agagaggtga
      841 ggatggatca ggaaattgaa gtcaagtttt acctgcaaga cctggcagct ttccgccagc
      901 gtttactttc agcgggggct tttcttactc aaccgcgcgt gaaggagatc aatctgcgct
      961 tcgatacccc gcagagggac ttgagccaaa aagcccaggt gctgcgcttg cggcaggatg
     1021 aggctgccca cctcaccttc aagggccccg gcgcgctaga aggtggggtg ctttcccgcc
     1081 aggaaatcga atttgaggtc agcgactttg ctgccgcgcg gcgcttcttg gaggcgctgg
     1141 gctaccgcgt tttcacgact tatgaaaaga ttcgcgaaaa ttttacgctg gcgggcgtgc
     1201 aggtttcgtt ggatgagatg ccctttggca actttaccga gttggaggga cccaaccccg
     1261 atgcgattcg caagttagcg gggagcttgg gcttagcttg ggagcgccgc attgccatga
     1321 gctacctaga gctttttgcc cggctcaaag cacgcctggc tttgcccttt gatgacctca
     1381 cctt
//
LOCUS       DAOTLW010000148         1378 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_77722_length_1378_cov_7.142857, whole genome shotgun sequence.
ACCESSION   DAOTLW010000148 DAOTLW010000000
VERSION     DAOTLW010000148.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1378)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1378
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_77722_length_1378_cov_7.142857"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /gene="cas5e"
                     /locus_tag="P5516_09395"
     CDS             <1..545
                     /gene="cas5e"
                     /locus_tag="P5516_09395"
                     /inference="COORDINATES: protein motif:HMM:NF021240.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein Cas5/CasD"
                     /protein_id="HRT92680.1"
                     /translation="LGCALGISADQDLADLSRGLRMGVRCEREGDFLTDFHTVIGGVI
                     SAEGKVKKDTVLSRRTYLCDASYLIALQGETGLIARLSKAVQKPVWPVYLGRRSCVPA
                     KNLYEGEGDFDDLLDALQRWPYQGKLDGENDRLRVVVEVDAGQGTQRRDEILSNSHRI
                     FLPRYTREHRIRPATLQEVD"
     gene            545..1147
                     /gene="cas6e"
                     /locus_tag="P5516_09400"
     CDS             545..1147
                     /gene="cas6e"
                     /locus_tag="P5516_09400"
                     /inference="COORDINATES: protein motif:HMM:TIGR01907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas6/Cse3/CasE"
                     /protein_id="HRT92681.1"
                     /translation="MYLALYEIKNSKVALGWIANRYRVHQRLRMAYRDEPRLLFRIEE
                     HDGNFRILAQSTCQPDWQHAFVDFDVLAGIPAVKELRLENLMPRSYYHFRLVANPVVT
                     RNRKRLGLFKEEAQFEWLKRQMERSGTIVMQAQARQLGLMRSAKNPAKDENRQTHYAV
                     EFNGVLQVLNVKLLSERMIEGIGPAKAYGFGLLTLARFTA"
     gene            1170..>1378
                     /locus_tag="P5516_09405"
     CDS             1170..>1378
                     /locus_tag="P5516_09405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92682.1"
                     /translation="MQNLRILPKLRDSLTYLYIEHAVIDQKHQAIEYVNKEGRVMVPV
                     ANICLLLLGPGTSITHAAIRTLMENG"
BASE COUNT          341 a          302 c          402 g          333 t
ORIGIN      
        1 tgctgggatg cgcgctcggt atttcagcag atcaagacct cgcggatttg agcagggggc
       61 tgcgcatggg tgtgcgttgt gaacgcgaag gagatttcct gacggatttt cacacggtca
      121 ttggcggcgt gatttcagca gagggaaagg tgaagaaaga tacggttctc tcgcggcgaa
      181 cctacctttg tgatgcctcg tatctgatag cgttacaggg tgaaactggg ctgattgcga
      241 ggctttccaa agcggtgcaa aaaccagtat ggcctgtgta cctgggaaga cgctcgtgtg
      301 ttccagcgaa aaacttatac gagggggaag gggattttga cgaccttctt gacgctcttc
      361 agcgctggcc atatcaagga aaactggatg gtgagaatga ccgtttgcgg gtcgtggtgg
      421 aggtagatgc cggacaggga acccagagaa gggacgaaat cttatccaat tcgcaccgta
      481 ttttcctgcc gcgctacacc cgcgaacaca ggatcagacc ggccaccctt caggaggtgg
      541 attaatgtac cttgcgctgt acgagatcaa gaactcaaaa gttgccctgg gatggatcgc
      601 caatcgctac cgtgtgcacc agagactgcg tatggcatac cgcgacgaac cgcgcctgct
      661 ttttcgcatt gaagagcatg atggaaactt tcgtatcttg gcgcaatcaa cttgccagcc
      721 tgattggcag catgccttcg tggattttga tgtgctggct ggaatccctg ctgtcaaaga
      781 actccgctta gaaaacctga tgccgagaag ttattatcat ttcaggcttg ttgccaaccc
      841 ggtggtcacc aggaacagaa aacgcctagg tttgtttaaa gaggaggcgc agtttgagtg
      901 gttgaagcgg cagatggaac gatccgggac aatagtgatg caggcgcagg cccgccagct
      961 ggggttgatg cgttctgcaa agaacccggc aaaggatgaa aaccgccaga cacactacgc
     1021 tgttgaattc aatggggtac tccaggtctt aaacgttaag ttgctttcgg aaaggatgat
     1081 tgagggtatt gggcctgcga aggcatatgg gtttggctta ttaacgctgg caaggttcac
     1141 cgcgtgagta cgattgggtt ttttttacca tgcagaattt acgtatactt ccaaaattac
     1201 gggatagcct gacttatttg tacattgagc atgccgtaat tgatcagaaa catcaggcaa
     1261 tcgaatatgt gaacaaagag ggtcgggtga tggttccagt ggcgaatata tgcctgctgt
     1321 tattaggccc cggcacgagt attactcatg ctgccatccg tacgttgatg gaaaacgg
//
LOCUS       DAOTLW010000149         1369 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_78500_length_1369_cov_9.353120, whole genome shotgun sequence.
ACCESSION   DAOTLW010000149 DAOTLW010000000
VERSION     DAOTLW010000149.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1369)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1369
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_78500_length_1369_cov_9.353120"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            18..>1369
                     /locus_tag="P5516_09410"
     CDS             18..>1369
                     /locus_tag="P5516_09410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT92683.1"
                     /translation="MTSKQQKITDDLDAMLSILPPDIAQSVQAEDNFDNLLEIILDLG
                     RKPAARFIDSEVILLPRDVTQYDLDYVTERIGSFDADNRAGLERTLHRISAIRNRRGE
                     IVGLTCRVGRAVYGTIDIIADLVNSGESILLLGKPGVGKTTMLREAARILAETKRVII
                     VDTSNEIGGDGDVPHPAVGRARRMQVPKPSLQHEVMIEAVENHNPEVIVIDEIGRELE
                     AQAARTIAERGVQLIGTAHGNALENLLLNPTLSDLVGGIESVTLSDDEARRRGTQKTV
                     LERRAPPTFTVLVEIQSRQHLAVYSDVAAAVDALLRGYPIPPEERSWDEEGKIRIQRA
                     EMRQAPTVNQSLRRGNGMEKTPFQRGISSDKSTRKATLALPSEADFENHVPANAQEVK
                     NIYAFGVARNRLLQVIKNMGVPARIVRNLEDADTFVTQRRYYRERLQPVIQAEEMSIP
                     V"
BASE COUNT          343 a          347 c          385 g          294 t
ORIGIN      
        1 aagtaataag caagaagatg acaagcaaac aacaaaaaat taccgatgat ctggatgcaa
       61 tgctctcaat tttgccgcca gacattgctc aatctgtaca agctgaagat aatttcgaca
      121 acctgctcga gattatttta gacttaggca ggaagccggc agcgcgcttt atcgatagcg
      181 aagtgatttt gctccctcgc gatgtgaccc aatatgactt ggattacgtc accgagcgca
      241 tcggcagctt tgatgcagat aaccgcgcag gcttggagcg cacgctgcat cgtatttccg
      301 ctattcgcaa tcggcgcggg gaaattgtcg ggctcacttg ccgggtaggc agggcggtat
      361 atggcactat cgatatcatt gcggacttgg tcaactccgg cgagagcatc ttgctcctcg
      421 ggaagcctgg tgtaggcaaa actaccatgc tgcgggaagc tgcgcgtatc ttggcggaaa
      481 ccaagcgtgt cataatcgtg gacacctcca acgaaatcgg tggggatggt gacgtcccgc
      541 atccggctgt tgggcgtgcc cggcgcatgc aggtgccaaa gccttcgttg caacacgaag
      601 tgatgattga agcggttgaa aaccataacc ctgaagtgat tgtcattgac gaaatcggac
      661 gggagctcga agcacaggca gcgcgcacca ttgccgaacg gggcgtgcag ctgattggca
      721 cagcgcatgg taatgcactt gaaaacctgc tgctcaaccc aacgctttcg gacctcgtgg
      781 gtgggattga atcggttacg ctttcagatg atgaagcccg ccggcgcgga acacaaaaaa
      841 cggtgctcga acggcgcgca ccaccaactt ttacagtttt ggtggagatt caaagccggc
      901 agcacttggc tgtttattcg gatgttgccg cagcggtaga cgcgttgctg cgcggctacc
      961 ccataccccc agaggaacgc tcctgggatg aagaaggcaa gattcgcatt cagcgtgctg
     1021 agatgcggca agcccccacc gtaaatcaat cgctcaggcg cggaaatggc atggagaaga
     1081 caccttttca gcgcggtatc agcagcgata agtccactcg gaaagcaacg cttgctctac
     1141 cctctgaggc tgacttcgag aaccatgtgc ctgccaacgc tcaggaagtg aagaatattt
     1201 atgcctttgg cgtagcgcga aaccgcttgt tacaggtgat taaaaatatg ggcgtgccag
     1261 cgcgtatagt tcgtaactta gaggatgcgg atacttttgt cacccagagg cgctactatc
     1321 gcgagcgctt gcaacctgtg atccaagcgg aagaaatgag cattccggt
//
LOCUS       DAOTLW010000150         1363 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_79030_length_1363_cov_8.480887, whole genome shotgun sequence.
ACCESSION   DAOTLW010000150 DAOTLW010000000
VERSION     DAOTLW010000150.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1363)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1363
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79030_length_1363_cov_8.480887"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            47..1192
                     /gene="cas7e"
                     /locus_tag="P5516_09415"
     CDS             47..1192
                     /gene="cas7e"
                     /locus_tag="P5516_09415"
                     /inference="COORDINATES: protein motif:HMM:NF020902.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I-E CRISPR-associated protein
                     Cas7/Cse4/CasC"
                     /protein_id="HRT92684.1"
                     /translation="MLIQFHILQNYAPSNLNRDDTGAPKDAVFGGVRRGRISSQCIKR
                     SIRRSTAFAENFSQDDLLGIRTRKLPSLIKEELKRQGVDEPDLSLIVDKLSGVGRKME
                     KKNQDDEGEIEGGAEVAEKEDMVKQLMFLAKNEISPMASALLETYKKMGAKEWGKADI
                     EKITKGLKTSLPRSVDIAMFGRMTTSDAFEDVSAAVQVAHAISTNALNQEFDYFTAVD
                     DLADEPGAGMIGDIEFNSSTYYKYVNVHWEELLANLGDDRAVAERGVLALLDAVAKAH
                     PTGKQNTFAAFNLPDFVLVEVTDVNLPVSYANAFLKPAKASHDATLMQNSIAQLEKYI
                     EKVTAAYNLHPKRACLNLDGEEFPRSERVKSLDELKNWLKACLPREV"
BASE COUNT          368 a          297 c          395 g          303 t
ORIGIN      
        1 tgggctcgtt cgtattttgg tcaataagtg aaaaaaggag acaatcatgt taattcaatt
       61 tcatatcttg cagaactatg cgccatctaa tttaaaccgt gatgataccg gcgcgccgaa
      121 agacgctgtt tttggtggtg taaggcgcgg gcgcatttcc agtcagtgca ttaaacgcag
      181 tatccgccgg tcaacagcct tcgcagaaaa tttttcgcaa gacgacctgc tgggcatccg
      241 caccagaaaa ttaccctctt tgataaaaga agagctcaag cggcaggggg tagatgagcc
      301 agacctgtcg ctcattgttg ataagttatc tggtgtgggt cggaaaatgg agaagaagaa
      361 ccaggatgat gaaggggaaa tcgaaggcgg cgctgaggtt gctgagaagg aggatatggt
      421 aaaacaactg atgtttctgg caaagaacga aatcagcccc atggcttctg cgctgctcga
      481 gacctataaa aaaatggggg cgaaagaatg gggcaaggcg gatattgaga agatcacgaa
      541 gggattgaag acaagcctgc ccaggtcagt ggatatagcc atgttcgggc gaatgaccac
      601 gtcggacgcg tttgaagatg tgagcgcggc agtacaggtt gcgcatgcga tttcgaccaa
      661 cgcactcaac caggaattcg attacttcac ggcagtggac gacctggcgg atgaaccggg
      721 tgccgggatg attggggata ttgagtttaa cagctcgacc tactacaagt atgtgaacgt
      781 gcactgggag gaactgttgg ccaacctggg agatgatagg gctgtagctg aacggggtgt
      841 gctggcttta ctggacgcgg tggcgaaagc tcatccaacc ggtaagcaga acacctttgc
      901 tgctttcaac ctgcctgatt ttgtgctcgt tgaagtcact gatgtcaatt tgccggtcag
      961 ttacgccaac gccttcctta aacctgcaaa agccagtcat gatgcgacgt tgatgcaaaa
     1021 ttccatcgcc caactggaaa aatacattga aaaagtaaca gcagcctaca atcttcaccc
     1081 aaaacgcgcc tgcctgaacc tggatgggga ggaatttcca aggtcggaac gagttaagtc
     1141 gctggatgag ttgaaaaact ggctaaaggc ttgcctgccg cgggaggtat gaaatggcca
     1201 ataccctgtt cttgaggctt gaaggacctc ttcaagcctg gggtgaacgg gcgcgctgga
     1261 gtgtacggga tacctccact gaacctacca aatctggggt agtggggatg ctgggatgcg
     1321 cgctcggtat ttcagcagat caagacctcg cggatttgag cag
//
LOCUS       DAOTLW010000151         1355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_79725_length_1355_cov_7.718462, whole genome shotgun sequence.
ACCESSION   DAOTLW010000151 DAOTLW010000000
VERSION     DAOTLW010000151.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79725_length_1355_cov_7.718462"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="P5516_09420"
     CDS             <1..275
                     /locus_tag="P5516_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HRT92685.1"
                     /translation="MISTLDGASYVVRRSLHDPKSIRLAKKAIRTAFEVQIRGLGFSL
                     VELLSTCSTNWGMTPVESMHWLENNMVPCFPLGDFKVHPAIAGLRF"
     gene            288..827
                     /locus_tag="P5516_09425"
     CDS             288..827
                     /locus_tag="P5516_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRT92686.1"
                     /translation="MQTEIITAGFGGQGVLFTGQLLTYAAMDEGREVTWIPSYGPEMR
                     GGTANCTVVISDEEIGSPMVSDPQAVIAMNLPSLDKYEGKVRPGGVLVVNESMVDRAV
                     SRQDIKVVMVKANEIAEELGDKRMMNMVLLGALIANLPVIPLEAIEKALAGHLPERHH
                     KMLTKNYEALKRGAAHLAQ"
     gene            complement(891..>1355)
                     /locus_tag="P5516_09430"
     CDS             complement(891..>1355)
                     /locus_tag="P5516_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92687.1"
                     /translation="AWFIVATSFSGMVYWLRANGWHTSKQAWRVWVYPVIALFGAIFI
                     LAATNFLFLHLFAHSDIGSAMAMVMLVYAGLVIVFITRPRLVPVRRPDWVVLLVPLVF
                     HLFFTIVGFTSGIYLNLSVLAVIGLLMFGLGLVVHLWPWYTHQVNQQQNKHK"
BASE COUNT          342 a          337 c          397 g          279 t
ORIGIN      
        1 aaatgatctc cacgctggat ggggcttctt acgtggtgcg gcgttcgctg cacgacccaa
       61 agagtatccg cctggcaaaa aaggctatcc gcaccgcgtt tgaagtacaa attcgcgggc
      121 tggggttttc cctggtggag cttctttcca cctgttcgac caattggggc atgaccccgg
      181 tggaatcaat gcactggtta gagaacaaca tggtgccttg tttccccctg ggcgatttta
      241 aagtccaccc ggcgatcgcc gggctcaggt tctaggcggg agggaaaatg caaacagaga
      301 tcattactgc aggttttggc gggcagggtg tgttattcac tggtcagctg ctcacatacg
      361 ccgccatgga tgaggggcgg gaggttacct ggataccctc ttatgggcct gagatgcgcg
      421 gcggaactgc gaactgcaca gtggtcattt ctgatgaaga aattggttcg ccgatggtca
      481 gcgatccgca ggctgtgatt gccatgaacc tgccttcatt ggataaatat gagggcaagg
      541 tcagacccgg cggagtgctg gtggtgaatg aatcgatggt tgatcgcgcg gtgtcccggc
      601 aggatatcaa ggtggtgatg gtaaaagcca atgaaattgc tgaagaactg ggtgataagc
      661 gcatgatgaa catggtactg ctcggcgcgc tcatcgccaa cctgccagtg attccgctgg
      721 aggcgattga gaaagcactg gcggggcacc tgccggagcg gcaccacaaa atgctaacga
      781 agaactatga ggcgctgaaa cgaggagcgg cgcatctggc gcagtaacaa ctggttcatc
      841 caatagaact ggagaagtcg tctcttttcc agttctattt ttttaagacg tcatttgtgc
      901 ttgttttgct gctgattgac ctgatgcgtg taccagggcc acaggtgaac aaccagcccg
      961 agcccgaaca tcaacaaccc gatgacagcc agaacgctta agttcaaata aatcccgctt
     1021 gtgaacccca cgattgtaaa aaacaggtga aagaccagtg gaaccaagag caccacccag
     1081 tcagggcgcc gcacggggac gagacgcggg cgggtgataa aaacgatcac caaaccggcg
     1141 tataccagca tcaccatggc catggcgctg ccgatatcgc tgtgtgcgaa tagatgcaag
     1201 aatagaaagt tggtggcagc aaggatgaag atggccccga acagggcgat taccgggtaa
     1261 acccacactc gccaggcctg tttggaagtg tgccaaccgt tcgctcgcag ccagtaaacc
     1321 atcccgctga aagaagtagc cacaatgaac caggc
//
LOCUS       DAOTLW010000152         1355 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_79726_length_1355_cov_7.331538, whole genome shotgun sequence.
ACCESSION   DAOTLW010000152 DAOTLW010000000
VERSION     DAOTLW010000152.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1355)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_79726_length_1355_cov_7.331538"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..275
                     /locus_tag="P5516_09435"
     CDS             <1..275
                     /locus_tag="P5516_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate oxidoreductase"
                     /protein_id="HRT92688.1"
                     /translation="MISTLDGASYVVRRSLHDPKSIRLAKKAIRTAFEVQIRGLGFSM
                     VELLSTCSTNWGMTPVESLHWLENNMVPYFPLGDFKVHPAIAGLRF"
     gene            288..827
                     /locus_tag="P5516_09440"
     CDS             288..827
                     /locus_tag="P5516_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HRT92689.1"
                     /translation="MQTEIITAGFGGQGVLFTGQLLTYAAMDEGREVTWIPSYGPEMR
                     GGTANCTVVISDEEIGSPMVSDPQAVIAMNLPSLDKYEGKVRPGGVLVVNESMVDRAV
                     SRQDIKVVMVKANEIAEELGDKRMMNMVLLGALIANLPVIPLEAIEKALAGHLPERHH
                     KMLTKNYEALKRGAAHLAQ"
     gene            complement(891..>1355)
                     /locus_tag="P5516_09445"
     CDS             complement(891..>1355)
                     /locus_tag="P5516_09445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92690.1"
                     /translation="AWFIVATSFSGMVYWLRASGWHTSKQAWRVWVYPIIALFGAIFI
                     LAATNFLFLHLFAHSDIGSAMAMVMLVYAGMVIVFITRPRLVPVRRPDWVVLLVPLVF
                     HLFFTVVGFTSGIYLNLSVLAVIGLLMFGLGLVVHLWPWYTHQVNQQQNKHK"
BASE COUNT          342 a          342 c          395 g          276 t
ORIGIN      
        1 aaatgatctc cacgctggat ggggcttctt acgtggtgcg gcgttcgctg cacgatccaa
       61 agagtattcg cctggcaaaa aaggctatcc gcaccgcgtt tgaagtacaa attcgcgggt
      121 tggggttttc catggtggag ctcctttcca cctgttcgac caattggggc atgaccccgg
      181 tggaatcact gcactggttg gagaacaaca tggtgcctta tttccccctg ggcgatttca
      241 aagtccaccc ggcgatcgcc gggctcaggt tctaggcagg agggaaaatg caaacagaga
      301 tcattactgc aggttttggc gggcagggtg tgctgttcac cggtcagctg ctcacatacg
      361 ccgctatgga tgagggacgg gaggttacct ggataccctc ttacgggcct gagatgcgcg
      421 gtggaactgc gaactgcaca gtggtcattt ctgatgaaga aataggttct ccaatggtca
      481 gcgatccgca ggctgttatc gccatgaacc tgccttcgtt ggataaatat gagggcaagg
      541 tcagacccgg cggagtgctg gtggtgaatg aatcgatggt tgatcgcgcg gtgtcccggc
      601 aggatatcaa ggtggtgatg gtaaaagcca atgaaattgc tgaagaactg ggtgataagc
      661 gcatgatgaa catggtactg ctcggcgcgc tcatcgccaa cctgccagtg attccgctgg
      721 aggcgattga gaaagcactg gcggggcacc tgccggagcg gcaccacaaa atgctaacga
      781 agaactatga ggcgctgaaa cgaggagcgg cgcatctggc gcagtaacaa ctggttcatc
      841 caatagaact ggagaagtcg tctcttttcc agttctattt ttttaagacg tcatttgtgc
      901 ttgttttgct gctgattgac ctgatgcgtg taccagggcc acaggtgaac aaccagcccg
      961 agcccgaaca tcaacaaccc gatgacagcc agaacgctta agttcaaata aatcccgctt
     1021 gtgaacccca cgactgtaaa aaacaggtga aagaccagcg gaaccaggag caccacccag
     1081 tcagggcgcc gcacggggac gaggcgcggg cgggtgataa aaacgatcac cataccggcg
     1141 tataccagca tcaccatggc catagcgctg ccgatatcgc tgtgtgcgaa taaatgcaag
     1201 aacaggaagt tggtggcagc aaggatgaag atggccccga acagggcgat tatcgggtaa
     1261 acccacaccc gccaggcctg tttggaagtg tgccaaccgc tcgctcgcag ccagtaaacc
     1321 atcccgctga aagaagtagc cacaatgaac caggc
//
LOCUS       DAOTLW010000153         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_80374_length_1348_cov_5.433875, whole genome shotgun sequence.
ACCESSION   DAOTLW010000153 DAOTLW010000000
VERSION     DAOTLW010000153.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80374_length_1348_cov_5.433875"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..230
                     /locus_tag="P5516_09450"
     CDS             <1..230
                     /locus_tag="P5516_09450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92691.1"
                     /translation="HPSCEGGSATGTHVHIARKYNGEWIEADSVVPFNLEGWIAKNGV
                     VPYQGSLSNYGRIITASDKGLVNSYITAGNP"
     gene            230..1234
                     /locus_tag="P5516_09455"
     CDS             230..1234
                     /locus_tag="P5516_09455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92692.1"
                     /translation="MHERTRSGQIRLESIRLIFGLLLLAMVTSGCVANPVPDEPAPGS
                     GQPTAASTLESNTLETSDAITAAVTATADLPATDSQTPPPQAEAEADWLSMPIVPTVS
                     PRMKDVFERGQKSGRDAARFSKIGDCQNITTYFLAMYDSGNYRLGDQYAYLQPTIDHF
                     KGSWWRQSLSVKGGMNVAAVLSPIWANPDKCLPKETPLACELRVYNPAFAVISLEESW
                     SGSIEHYDMYLREIVEYVLAQDIVPILATRAETETQERQINPTVARIAHDYQVPLWNF
                     GAAARALPNNGIRPDGFHITEGQSYFDDEAMLKTGWTQRNLTALQAIDAVYRGLTQEP
                     "
BASE COUNT          317 a          377 c          370 g          284 t
ORIGIN      
        1 ggcacccttc atgcgaaggc ggttccgcca ccggcacaca tgtgcatatc gcccgtaaat
       61 acaatggcga atggattgaa gcggatagcg tggtgccatt caacctggaa gggtggatcg
      121 cgaaaaacgg ggtggtccct taccagggaa gcctgagcaa ttatgggcgt atcatcaccg
      181 ctagcgacaa gggcctggta aattcctata tcaccgcggg gaatccataa tgcacgaacg
      241 aacccgttct ggtcagattc ggctggaatc gatacgcttg attttcggct tgttactgct
      301 agctatggtc accagcggct gcgtggccaa ccctgtgcct gatgagcccg cgcctggttc
      361 cgggcaacca actgccgcca gtacactgga aagcaacacg ttggaaacgt cggacgcgat
      421 caccgcggcg gttactgcaa ccgctgactt gccagccacg gattcacaaa cgccgccccc
      481 ccaggcggaa gctgaagcag actggctctc catgcccatc gtgccgacag tatccccccg
      541 gatgaaagac gtttttgaac gcggacagaa aagcggtcgc gatgccgcgc gtttttcaaa
      601 aataggcgat tgtcaaaaca tcacgaccta cttcctggcg atgtatgaca gcggtaatta
      661 ccggctggga gatcaatacg cttaccttca gccaacgatt gatcatttta aaggctcctg
      721 gtggcgccag agtctctcag tgaaaggcgg gatgaacgta gcagcggtgc tcagcccgat
      781 ttgggcgaac ccggataagt gcctgcccaa agaaactccc ctggcctgtg agctgcgtgt
      841 gtacaacccg gcgtttgcgg tcatcagcct ggaagaatcc tggtcaggca gcattgagca
      901 ttatgacatg tacctgcgcg aaattgtgga atacgtgctg gcgcaggaca tcgttcccat
      961 cctcgccacg cgcgcggaaa ctgaaaccca ggaaaggcaa atcaacccta ccgtggcgcg
     1021 catcgcgcat gattaccagg tgcctttgtg gaacttcggc gccgcagcca gagctctacc
     1081 taataatggg attcgaccgg acgggttcca tattaccgag gggcaatctt actttgatga
     1141 cgaggccatg ttaaaaacag gctggaccca acgcaacctc accgctctgc aggcgatcga
     1201 tgccgtctat cgtggactga cccaggagcc ataaccttac gcggttttta aggtaaggtg
     1261 cgcaagcctg ttttcccagg caggttaggc ttgttcaatc gtatatttgg tttgaatatt
     1321 ggctgccttt cgcagcatcg ccataccg
//
LOCUS       DAOTLW010000154         1348 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_80377_length_1348_cov_4.677494, whole genome shotgun sequence.
ACCESSION   DAOTLW010000154 DAOTLW010000000
VERSION     DAOTLW010000154.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1348)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1348
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80377_length_1348_cov_4.677494"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(115..1119)
                     /locus_tag="P5516_09460"
     CDS             complement(115..1119)
                     /locus_tag="P5516_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92693.1"
                     /translation="MNEQIRTGQARLGAIRSIFGLVLLVMVTSGCVANPVPDVPAPGS
                     GQPTTAHTQESNALEAASTITAAVTATADMPGTDSQTLPPQADAETDWLSMPIVPTVP
                     PRMKDVFERGQKSGRDAARFSKIGDCQNITTYFLAMYDSGNYRLGDQYAYLQPTIDHF
                     KGSWWRQSLSVKGGMNVAAVLSPIWANPDKCLPKETPLACELRVYNPAFAVISLEESW
                     SGSIEHYDMYLREIVEYVLAQDIVPILATRAETETQERQINPTVARIAHDYQVPLWNF
                     GAAARTLPNNGIRPDGFHITEGQSYFDDEVLLITGWTQRNLTALQAIDAVYRGLTQEP
                     "
     gene            complement(1119..>1348)
                     /locus_tag="P5516_09465"
     CDS             complement(1119..>1348)
                     /locus_tag="P5516_09465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92694.1"
                     /translation="HPSCEGGSATGTHVHIARKYNGEWIEADSVVPFNLEGWVAKNGT
                     VPYQGSLSNFGRVITASDKGLANSYITAGNP"
BASE COUNT          281 a          367 c          382 g          318 t
ORIGIN      
        1 cggtatggcg atgctgcgaa aggcagccaa tattcaaacc aaatatacga ttgaagaggc
       61 ctaacctgcc tgggaaaacg ggcttgcgaa ccttatctta aagaccgcgt aaggttatgg
      121 ctcctgggtc agtccacgat agacggcatc aatcgcctgc agagcggtga ggttgcgttg
      181 ggtccagccg gttattaaca aaacctcgtc atcaaagtaa gactgcccct cggtaatatg
      241 gaacccgtcc gggcgaattc cattattcgg cagagttctg gctgcggcgc cgaagttcca
      301 taaaggcacc tggtaatcat gcgcgatacg cgccacggtg gggttgattt gcctttcctg
      361 ggtttcagtt tccgcgcgcg tggcgaggat gggaacgatg tcctgcgcca gcacgtattc
      421 cacaatttcg cgcaggtaca tgtcataatg ctcaatgctg cctgaccagg attcttccag
      481 gctgatgacc gcaaacgccg ggttgtacac acgcagctca caggccaggg gagtttcttt
      541 gggcaggcac ttatccgggt tcgcccaaat cgggctgagc accgctgcta cgttcatccc
      601 gcctttcact gagagactct ggcgccacca ggagccttta aaatgatcaa tcgttggctg
      661 aaggtaagcg tattgatctc ccagccggta attaccgctg tcatacatcg ccaggaagta
      721 ggtcgtgatg ttttgacaat cgcctatttt tgaaaaacgc gcggcatcgc gaccgctttt
      781 ctgtccgcgt tcaaaaacgt ctttcattcg ggggggtact gtcggcacga tgggcatgga
      841 gagccagtct gtttcagcat ccgcttgggg gggcagcgtt tgtgaatccg tgcctggcat
      901 atcagcggtt gcagtaaccg ccgcggtgat cgtgctcgcc gcttccaacg cgttgctttc
      961 ctgtgtatgg gcggtagttg gctgcccgga accaggcgcg ggcacatcag gcacagggtt
     1021 ggccacgcag ccgctggtga ccataaccag cagtaccaag ccgaaaatag agcgtatcgc
     1081 tccaagccga gcctgacctg tacggatttg ttcgttcatt atggattccc tgcggtgata
     1141 taggaatttg ccaggccttt gtcgctggcg gtgatgacgc gcccaaaatt gctcaggctt
     1201 ccctggtaag ggaccgtccc gtttttcgcg acccaccctt ccaggttgaa tggcaccacg
     1261 ctatccgctt caatccattc gccattatat ttgcgggcga tatgcacatg tgtgccggtg
     1321 gcggaaccgc cttcgcatga agggtgcc
//
LOCUS       DAOTLW010000155         1346 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_80567_length_1346_cov_5.897754, whole genome shotgun sequence.
ACCESSION   DAOTLW010000155 DAOTLW010000000
VERSION     DAOTLW010000155.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1346)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1346
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_80567_length_1346_cov_5.897754"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="P5516_09470"
     CDS             complement(<1..595)
                     /locus_tag="P5516_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HRT92695.1"
                     /translation="MNQRVIYPFTAIVGQERMRRALVLNAVDPRIGGVLIRGERGTAK
                     STAARALAALLPEEEAIEGCRFGCDPHDPTHWCTECIERAAASKEPLPIIRRPTPFIN
                     LPVSATEDRVVGTLDIERAIQKGERHFEPGVLAAANRGLLYIDEVNLLDDHVVDILLD
                     SAAMGMNIVEREGISFSHPARFILVGTMNPEEGDLRPQ"
     gene            complement(970..>1346)
                     /locus_tag="P5516_09475"
     CDS             complement(970..>1346)
                     /locus_tag="P5516_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HRT92696.1"
                     /translation="ACQQAGITPAQADFFEYADITTLHAVLSLEAAGLAPRGQGWRLA
                     RDHAQWQHKPLPVATLGGYKARGNPLGAAGVYQAVEACLQLRGEAGATQLPQARVGMI
                     QALAGTGSTALTHVLMQVEETN"
BASE COUNT          310 a          369 c          369 g          298 t
ORIGIN      
        1 gctgcggccg cagatcgcct tcctcagggt tcatcgtgcc tactaagatg aagcgagcgg
       61 ggtgactaaa agaaatgcct tcgcgctcga cgatgttcat ccccatcgcg gcagaatcca
      121 gcaaaatatc gacgacatgg tcatccagca ggttgacttc atcaatatac agcagcccac
      181 ggttggctgc cgctaaaacg cccggctcaa agtggcgttc gcctttttgg atggcacgct
      241 cgatatccag cgtcccgacc acacggtcct ctgtggcaga aaccggcagg ttgataaagg
      301 gcgttgggcg gcggatgatg ggtaaaggct ccttggaagc cgctgcgcgc tcgatgcact
      361 cggtgcacca atgggttgga tcgtgcgggt cgcagccaaa gcggcagcct tcgatggctt
      421 cctcctctgg tagcaatgcc gctaaagcgc gcgcagcggt ggatttagct gtaccgcgct
      481 cgccgcgaat gagcacgccg ccaatgcgcg gatcaaccgc gttgagcacc agggcacggc
      541 gcatgcgctc ctgcccgaca atggcagtga atggataaat gactcgctgg ttcatcaaat
      601 tctttacctc aaaactaagt ttaccacgcc gaggggggaa atgagcgtag gcaagtatag
      661 tgttgcattt tcaacagttc atatataaga gagatgtaga aaaatcgaaa gttcctcgcg
      721 ttttttatgg taacttacgg ccagggatcg cactctacaa accatgcacg gtgtgcgacc
      781 tttaggtctc aaataagctc tcttccaatt atggtaatcg ttaatattaa atgtttggaa
      841 taacgaggga gacctgtggt caagttcctc gctcggtacc ctacgggcag gctacgcact
      901 caggagaccg gcaagaacat atcatgttta atttttcatt tcctacttat acatatttag
      961 aagtaaagct taattcgttt cttctacctg cataagaaca tgcgtcaatg ccgtggaacc
     1021 agttccggcc agagcctgga tcatccctac acgtgcctga ggcagctgcg ttgcgccagc
     1081 ctcaccgcgc agctgcaggc aagcttcgac tgcctgatag actcccgccg cgcccagcgg
     1141 attgccacgt gctttatacc cgccgagtgt ggctactggc aggggtttat gctgccactg
     1201 ggcgtgatcg cgggctaagc gccaaccttg cccgcgcgga gccaggcccg ctgcctcaag
     1261 cgaaagaacc gcatgcagcg tggtgatgtc tgcatattca aagaagtccg cctgagcagg
     1321 ggtaatgccg gcttgctgac aggcgc
//
LOCUS       DAOTLW010000156         1323 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_82660_length_1323_cov_8.400631, whole genome shotgun sequence.
ACCESSION   DAOTLW010000156 DAOTLW010000000
VERSION     DAOTLW010000156.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1323)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1323
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82660_length_1323_cov_8.400631"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..422)
                     /locus_tag="P5516_09480"
     CDS             complement(<1..422)
                     /locus_tag="P5516_09480"
                     /inference="COORDINATES: protein motif:HMM:NF014409.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NTP transferase domain-containing protein"
                     /protein_id="HRT92697.1"
                     /translation="MSQDNIIAIIQARMGSSRLPGKVLRDLGGEPVLARVINRVLRAK
                     TLTGVVVATTTDPVDDPIAAFCQQQAVPVFRGSQFDVLDRYYQAAREHHADVVVRITA
                     DCPMIDPQEIDRVVTAFLEGAYDFAANRLPPPAQRTSP"
     gene            complement(437..1090)
                     /locus_tag="P5516_09485"
     CDS             complement(437..1090)
                     /locus_tag="P5516_09485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92698.1"
                     /translation="MKKKWTLWLMSCTLLLTISACNLPGLLQTPATPVPTPTPDLGMV
                     WYQGAGMQILLPDTYAQRNVEQDLAGIWDSLSQFVGGQDSPLASLLDDLESNVAWWGE
                     DTAAPAVSPTQLLIVKNEALEGVPISLIKSGVKMFLGNDADALQTSDLTMGKREVTRF
                     TYGKESTGWSAYVFKEQGMLWVVLFITPPANLAAQQDDFDYSVSSIVIEAPTPTPQP"
     gene            complement(1087..>1323)
                     /locus_tag="P5516_09490"
     CDS             complement(1087..>1323)
                     /locus_tag="P5516_09490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92699.1"
                     /translation="DIPANLEWIREGENGWIFPDGDDQALAEVILKAAALDWRALSLQ
                     ARQDAVERADWSKNFQKLLKCYQDTVAYGKARKA"
BASE COUNT          291 a          376 c          365 g          291 t
ORIGIN      
        1 attgggctgg tgcgctgggc gggcggcggc aagcggttag cagcgaagtc atacgcgcct
       61 tccagaaagg cggttaccac acggtcaatc tcctgcgggt cgatcatcgg gcaatcggcg
      121 gtgatacgca cgaccacatc ggcatgatgt tcacgagcag cttggtaata gcggtctagg
      181 acgtcaaact ggctgccacg aaaaaccggc actgcctgct gctggcaaaa cgcagcgata
      241 ggatcatcca ccgggtcggt agtggtggca accacaaccc cagtgagggt cttggcgcgc
      301 aggacgcggt tgattacgcg tgccagcacc ggctcgccgc ccaagtcacg caggactttg
      361 ccgggcagcc ggctggaacc catgcgggct tgaatgatgg cgatgatatt atcttggctc
      421 ataagctcca tcggcttcag ggctgcggcg ttggggtggg cgcttcaatc actatggaag
      481 agacgctgta atcaaaatca tcttgctggg cagccaaatt ggctggcggt gtgataaaca
      541 acaccaccca gagcatccct tgctccttga aaacgtaagc cgaccagccg gtgctctcct
      601 taccgtaggt gaagcgggtt acttcacgct tgcccatagt taggtcggag gtttgcaagg
      661 cgtccgcgtc attccctaag aacattttga ccccggattt aatgagacta atcggaacac
      721 cttccagcgc ctcattcttg acgatcagca attgcgttgg cgaaaccgcc ggcgcagccg
      781 tatcttcgcc ccaccaggcg acattgctct ccaagtcatc cagtaatgag gctaacggtg
      841 aatcttgccc gccgacaaat tgagatagag aatcccagat tccggctaaa tcctgctcga
      901 cgttacgctg tgcgtaggta tctggcagca gaatttgcat gccggcgccc tggtaccaga
      961 ccatgcctaa atctggtgta ggggtcggca ctggggttgc cggagtctgc aataaaccag
     1021 gtaaattgca agctgagatg gtcagcagca aggtgcagct catcagccat aaagtccatt
     1081 tctttttcat gctttcctcg ctttcccgta agcaacggta tcttgataac attttaagag
     1141 cttttggaaa ttcttgctcc agtcggcacg ctccacggcg tcctgccggg cttgcaggct
     1201 cagcgcgcgc caatccagcg ctgccgcttt caaaataacc tccgctagtg cctggtcatc
     1261 cccatccggg aagatccaac cgttctcgcc ctcgcggatc cattctaaat tggcggggat
     1321 atc
//
LOCUS       DAOTLW010000157         1321 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_82847_length_1321_cov_10.653239, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000157 DAOTLW010000000
VERSION     DAOTLW010000157.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1321)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_82847_length_1321_cov_10.653239"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="P5516_09495"
     CDS             <1..291
                     /locus_tag="P5516_09495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92700.1"
                     /translation="PMLTGEEPQRIEIRVTKTEDQSAMAGIEASLELLLPDGEHYSSD
                     FPPTAVDGKAVLEIVTPKKALNGSILTYQVCLKNPALEPVCAQGSYLLWKTP"
     gene            complement(295..1260)
                     /locus_tag="P5516_09500"
     CDS             complement(295..1260)
                     /locus_tag="P5516_09500"
                     /inference="COORDINATES: protein motif:HMM:NF013630.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM domain-containing protein"
                     /protein_id="HRT92701.1"
                     /translation="MKVIKGLLMCLLLFMLSACGAGKQTGTAQPGEISKLTPYYTATF
                     TATVTPTPQGLPTSTPAPTATPTPRVYKVQPNDTLLGIANYFKISLEELKAANPGVQP
                     ALLPIGTELIIPASAAVATVVAPAPAAYTVNLLETKCVKSLTGGYHCFALVKNAEDFP
                     LANLTAEFTLSHSAGAETLSRTLELPLKRLPAGARLPFYTYFSPPVFTDAQATARLLT
                     MTDAHDEKETTIPLVIEEANTTISADGVAATVSGKAHFAEETAATAGSYTLVGVAYDV
                     NGQVVGLRRISAENEWSANATIDFSLSVYSTGGKIDRVEVFGEAQ"
BASE COUNT          288 a          358 c          363 g          312 t
ORIGIN      
        1 cccatgctca caggtgaaga accccaacgt attgaaatcc gggtcacaaa aactgaagat
       61 cagtcagcaa tggccggcat tgaggcctcg ttggagctgc tcttgccgga tggagaacat
      121 tacagcagcg attttccgcc taccgcggtt gacggtaaag cagttctcga aatcgttacg
      181 ccaaaaaaag cgcttaatgg ctcgatcctc acctatcaag tgtgccttaa gaaccccgca
      241 ttagaaccgg tatgcgcgca gggcagctac ctgctctgga agaccccctg agccctattg
      301 ggcttcgccg aaaacctcaa cccggtcaat ttttcccccg gtgctgtaaa cagacagtga
      361 aaaatcgatg gtcgcgttcg cgctccactc gttttctgcg cttatcctgc gcaggccaac
      421 cacctgccca ttaacatcat acgccacgcc gaccagcgtg tagctgccag cagtagcagc
      481 ggtttcttca gcaaagtgag ccttgccgct gaccgttgca gcaaccccgt ctgctgaaat
      541 ggttgtattt gcctcttcaa tcaccagagg gattgttgtt tctttttcgt cgtgggcgtc
      601 tgtcatggtg agaagccgcg ccgtcgcctg cgcatcggta aagaccggag ggctgaaata
      661 ggtgtaaaaa ggcaggcgcg cgccggctgg caaccgtttc aatggcagct caagcgttcg
      721 cgaaagcgtt tctgctcccg ctgagtggct gagggtaaac tccgcggtca ggtttgccag
      781 tgggaaatct tctgcgtttt tcaccagcgc aaaacaatga tacccgcctg tcagagactt
      841 cacgcatttt gtttccagca ggttaaccgt gtaagcggcg ggagcgggcg caaccactgt
      901 cgcgaccgcc gcggacgcag gaatgatcaa ttccgttcca atgggaagca gcgccggctg
      961 cacaccaggg ttagcggcct ttaactcttc gagcgatatc ttaaagtagt tggcaatgcc
     1021 aagcagcgta tcgttaggct gaactttata gacccttggt gtgggggtgg cggttggtgc
     1081 tggggtgctg gtgggcaatc cttgaggggt tggcgtgacc gttgcagtga aggtcgcggt
     1141 gtaataaggc gtcagttttg atatctctcc cggttgggcc gttcccgtct gttttccagc
     1201 cccgcaggct gaaagcatga agagaagcag acacatcagc aatcctttaa tcactttcat
     1261 attgcgcaat gaatcctccc cgcagcaagc tgcggggtat cgtctttaga ttttttcctt
     1321 g
//
LOCUS       DAOTLW010000158         1311 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_83824_length_1311_cov_8.548567, whole genome shotgun sequence.
ACCESSION   DAOTLW010000158 DAOTLW010000000
VERSION     DAOTLW010000158.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1311)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1311
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_83824_length_1311_cov_8.548567"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..275)
                     /locus_tag="P5516_09505"
     CDS             complement(<1..275)
                     /locus_tag="P5516_09505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92702.1"
                     /translation="MNPLQNLRDVIGRIERQLKAPVAQRDKFEREMERKLGRIWKEQR
                     DELMRLLGDPPSLSNVPQSYWNNGRAAIRKVIAPIFEEIFREQATAL"
     gene            complement(250..>1311)
                     /locus_tag="P5516_09510"
     CDS             complement(250..>1311)
                     /locus_tag="P5516_09510"
                     /inference="COORDINATES: protein motif:HMM:NF016732.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HRT92703.1"
                     /translation="FSQAGKVWPESDMVYIKEFSYSDDLTSGISTVQACLNDAGLMNY
                     QTRFASRFFEAGAMPIVLVSADGLIEEEKQRIQNFFSKLASGVGNAWRALATRTKLTP
                     EVVSQDLDKMTMPELYAQATKNIANAFGIPVNMFSGDDNYASADSHRMQFWQDTVRPR
                     GRIVEEALNRQVLKPMGLRMEFAFDEMDIFQEDETQRAQAFSLYVEAGVNPMVAKEML
                     GIESNTDIPFMAPQPEPIETEKPLDVTVDVTAEFEKWERKALKRIKDGKSADCQFDSE
                     LIPLAIQDEIHAALKLCVEPDEVKRVFGGEYESPQDIGLYKELKRANELLERSLMDKP
                     EFHITVNTKDVDEPITEPA"
BASE COUNT          271 a          416 c          289 g          335 t
ORIGIN      
        1 atcagcgcgg ttgcctgctc tctaaatatt tcctcgaata taggcgcgat caccttgcga
       61 atcgccgccc tgccattatt ccagtaggat tgcggcacgt tggaaagaga gggagggtca
      121 cccagtaagc gcatcaattc gtcacgctgt tccttccaga tacgcccaag tttgcgctcc
      181 atctcgcgct cgaatttatc tcgctgtgca accggcgctt tgagctggcg ttctattcgc
      241 ccgatcacgt cacgcaggtt ctgtaatggg ttcatccacg tccttcgtgt ttaccgtgat
      301 atgaaattcc ggcttatcca tgagagagcg ttccagtaac tcatttgccc gcttgagttc
      361 cttgtatagc cctatgtcct gcggtgactc atactcgcca ccgaataccc gcttgacctc
      421 atcaggttca acgcacaatt tcagcgcggc atgtatttcg tcctggatag caagcggtat
      481 cagttcgcta tcaaactggc agtccgcgct cttcccgtcc ttgatgcgct tcagcgcctt
      541 gcgttcccac ttctcaaatt cagccgtcac atccaccgtc acatccaaag gcttctcagt
      601 ttcaatcggc tccggttgcg gcgccatgaa cggaatatcg gtattgctct caatgcccag
      661 catttcctta gccaccatcg gattgacccc agcctcaacg tacaggctga acgcctgcgc
      721 ccgctgtgtc tcgtcctcct ggaatatatc catctcatca aacgcgaact ccatgcgcaa
      781 gcccatcggc tttaatacct ggcggttcaa cgcctcctca acaatgcgcc cgcgaggtct
      841 taccgtatcc tgccaaaact gcatccggtg tgagtccgcg ctcgcataat tgtcgtcacc
      901 actgaacata ttgacaggaa ttccaaacgc gttagctata ttctttgtcg cctgcgcgta
      961 caattcaggc atggtcatct tgtccaggtc ttgacttacc acctccggcg ttagcttcgt
     1021 gcgtgttgcc agcgcgcgcc acgcattacc aaccccgctt gccagtttgc tgaagaaatt
     1081 ctggatgcgc tgcttctcct cctcaatcaa accatccgcg ctaaccagca caatcggcat
     1141 tgcccccgcc tcaaagaacc tggacgcaaa tcgcgtctgg tagttcatca atccagcgtc
     1201 attcaagcac gcctgcacgg ttgagatccc gcttgtcagg tcgtcggagt aactgaattc
     1261 cttgatgtaa accatgtcgg attccggcca caccttgccc gcctgcgaga a
//
LOCUS       DAOTLW010000159         1308 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_84106_length_1308_cov_9.424581, whole genome shotgun sequence.
ACCESSION   DAOTLW010000159 DAOTLW010000000
VERSION     DAOTLW010000159.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1308)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1308
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84106_length_1308_cov_9.424581"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1258)
                     /locus_tag="P5516_09515"
     CDS             complement(<1..1258)
                     /locus_tag="P5516_09515"
                     /inference="COORDINATES: protein motif:HMM:NF013481.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HRT92704.1"
                     /translation="MATRLCWENITPGCDPYSPENPVCIANGIFTGTPVPVGGKFGMA
                     DKSPMTGFIGDSLSGSWFSIALKRAGWDGIVIHNQSDSWVNVFIDDDHVEFLDATYLL
                     GKGTYETEEAIREKLGDEQIRSCTIGPCGEHGVRFANVTNDGRQAGRTGHGSVWASKK
                     LKALSVRGTKGVRVYDPQTLMQLSYDISKAAQGEHTAKYRIYGTATGMKNSSAMGVLP
                     TRNFQEGVFEDIDLISGEYLDEHHKVKVISCAQCPIACEQMSIVKTGPFAGAMTGVEY
                     ESLQANSANLGISDIRASIQLLEIADHDGMDSMTMGVTISYAMECYERGVLKKEDFAC
                     KSYPEGLEMRFGNAEAAIALAKMIRDREGIGDILSNGTRIASKIIDAERGTESWKWAM
                     NTKGLECPGYDARSLKTFAIGLAVGTR"
BASE COUNT          291 a          356 c          337 g          324 t
ORIGIN      
        1 cgcgcgtacc aacggctaaa ccgatggcaa aggttttaag cgagcgggcg tcatatccgg
       61 ggcattccaa gcctttggtg ttcattgccc atttccagga ttccgtaccg cgttcagcat
      121 caataatctt gctggcaatg cgggtgccat tcgaaaggat gtcgccaatg ccttcgcggt
      181 cgcggatcat cttagcgagt gcgatagctg cttcagcgtt gccaaagcgc atttccaacc
      241 cttcagggta ggatttacag gcaaagtctt cttttttcag cacaccgcgc tcgtagcatt
      301 ccatggcgta agaaatggta acacccatgg tcatgctatc cataccatcg tggtcggcaa
      361 tctccagcag ctgaatgctg gcgcgaatat ccgagatgcc caagttagcg ctattggctt
      421 gcaggctttc atattccaca ccggtcatag caccagcgaa gggacctgtc ttgacgatgg
      481 acatttgctc gcaagcgata gggcattgtg cgcaggaaat caccttgacc ttgtggtgtt
      541 cgtccagata ttccccgctg atgaggtcaa tatcttcaaa aacgccttct tggaagttac
      601 gggtgggtaa aacacccatc gcactggaat ttttcatgcc agttgcggta ccgtaaatgc
      661 ggtatttggc tgtgtgttcg ccctgggctg ccttcgaaat atcataagaa agctgcatta
      721 atgtttgggg atcatacacc cggacgcctt tggtaccacg tacagagagc gccttcagct
      781 tcttcgaagc ccacacggag ccatgccctg tgcgccccgc ttgtcggcca tcgttggtca
      841 cgttggcaaa gcgtacgcca tgctcaccgc agggaccaat ggtgcaggag cgaatctgtt
      901 catcgcctaa tttttcccgg atagcctctt cggtttcata cgtgcctttg cccagcaggt
      961 atgtggcatc caggaactcc acgtggtcgt cgtcgataaa gacgttcacc cagctatcgc
     1021 tttggttgtg tatgacgata ccatcccagc cggcacgctt gagagcaatg gaaaaccaac
     1081 tgccggaaag actatccccg ataaaacccg tcattgggga tttatccgcc atgccaaatt
     1141 tgccgcccac gggaactgga gtccccgtaa agatgccatt ggctatacaa acggggtttt
     1201 caggggaata ggggtcgcag ccaggggtga tgttttccca gcagaggcgt gtcgccatgg
     1261 agaccccgcc gatccatagc ttataccatt cttctggttt ttcctcga
//
LOCUS       DAOTLW010000160         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_84574_length_1303_cov_9.991186, whole genome shotgun sequence.
ACCESSION   DAOTLW010000160 DAOTLW010000000
VERSION     DAOTLW010000160.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84574_length_1303_cov_9.991186"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(149..>1303)
                     /locus_tag="P5516_09520"
     CDS             complement(149..>1303)
                     /locus_tag="P5516_09520"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose/GDP-mannose dehydrogenase family
                     protein"
                     /protein_id="HRT92705.1"
                     /translation="MEEADLAHTDVVFIAVGTPSLPDGNADLTYVRQAAEAIAEAMDD
                     HFLVIVNKSTVPIGSGNWVGTLIKDAYEKRNGKKAEGKFAVVSNPEFLRQGAALHDSL
                     YPDRVVIGSENTHGVETLRELFTPILQQSFTPPPGLPRPEGLHEVPLLVCDLTSAEVI
                     KYAANAFLALKISYINEIAHLAQKVGADATLIAKGIGLDSRIGERFLNPGIGWGGSCF
                     GKDTAALVATAKEYNLAMPIVQAARDVNYHQRTWVVDALQDALKILKGKRVALLGFSF
                     KPNTDDLRDAPSLDIARLLVQRGASVRAHDPVALDNARRLYPDLGVRYCSQVEEALED
                     ADAIVLVTEWPLYRELDWEHIKPVPLVDGRNFLDRERLSALGFKVIGVGR"
BASE COUNT          285 a          411 c          326 g          281 t
ORIGIN      
        1 attcgaacag aaactgcatc gcacttgcca aacccttcgt taaccgcttt caacctctct
       61 tatacctatc gagaagtatt attctcataa gtaatgctaa ttactccaaa aaagcttaac
      121 tttccctccc ccatttcgct tttataggct accgaccgac tccgatgact ttgaatccta
      181 gcgcgcttag gcgttcgcgg tccaaaaagt tccgtccatc cactagcggc actggcttga
      241 tgtgctccca atccagctcg cgatacaggg gccactcggt gaccaagacg atcgcgtcgg
      301 catcttccag cgcctcttcc acctgagagc aataccgcac gcccaggtcc gggtacagcc
      361 ggcgggcatt atctaatgcc accgggtcat gcgcacgcac gctagcaccg cgttgcacca
      421 gcagccgggc aatatctaat gaaggcgcat cgcgcaggtc atccgtattg ggtttgaagc
      481 tgaagcccag cagcgccacg cgcttgcctt tgaggatctt gagcgcatct tgcagggcat
      541 ccaccaccca cgtgcgctgg tgatagttta cgtcgcgcgc ggcttgcaca atgggcatgg
      601 ctaagttgta ctccttagcc gttgccacca acgccgccgt atctttgcca aagcaagacc
      661 caccccagcc aatgcccggg ttgagaaagc gctcgccaat acggctatcc aagccaatac
      721 ccttagcaat cagcgtggca tccgcaccca ccttttgcgc taggtgcgca atctcattga
      781 tatacgaaat tttcagcgcc aaaaaggcgt tggctgcgta tttgatcacc tccgcactgg
      841 tcaggtcgca gaccagcagc ggcacctcat gcaagccctc agggcgcggc aagccaggcg
      901 gtggtgtaaa gctctgctgc aggatgggcg tgaaaagctc ccgcaaggtc tccacgccat
      961 gggtgttctc ggaaccgatc accacacggt ccgggtaaag cgaatcgtgc agcgctgctc
     1021 cttggcgcag gaactcgggg ttggaaacca ccgcaaactt gccctcagct tttttgccgt
     1081 tgcgcttttc gtaagcgtcc ttgatgagcg tacctaccca gttgccggag ccaatcggca
     1141 cggtggactt attcacaatc accaaaaagt ggtcgtccat cgcctctgcg atcgcctcgg
     1201 cggcttggcg cacataggtc aggtcagcgt tgccatccgg cagcgaaggt gtgccgaccg
     1261 caatgaacac cacatccgta tgagccaggt ctgcttcctc cat
//
LOCUS       DAOTLW010000161         1303 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_84576_length_1303_cov_9.170673, whole genome shotgun sequence.
ACCESSION   DAOTLW010000161 DAOTLW010000000
VERSION     DAOTLW010000161.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1303)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1303
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_84576_length_1303_cov_9.170673"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..649)
                     /locus_tag="P5516_09525"
     CDS             complement(<1..649)
                     /locus_tag="P5516_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT92706.1"
                     /translation="MAKAYSRYVCQVCGKVTSQPFGRCPACDSWDSMVEEVVEPESSH
                     SARTVRGLNASSQPRRLSEIDGQAEDRLPVAMEEFARVLGGGIVPGSIVLIGGDPGIG
                     KSTLTLQMAMQLAEENTVLYVSGEESERQIKMRADRLAPRDGAGKAHLPEKLYLVTET
                     NLSQIFTHVQTVQPALLVVDSIQTMTLPEMESTAGSISQVRECTARLRELAKSTGI"
     gene            complement(657..>1303)
                     /locus_tag="P5516_09530"
     CDS             complement(657..>1303)
                     /locus_tag="P5516_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HRT92707.1"
                     /translation="AHPEALQLLLQIMEEGHLTDAKGHKVDFRNAIIIMTSNIGADLI
                     KRQASMGFSLEVDQALEEQSSYEEMRKKLMDALKRTFRPEFINRLDSVVVFRALNQED
                     IRKIVNLELQKVADRLSDYDLTLTTTEAALDYLANEGFDPEMGARPVRRVIEQMVEEP
                     LADALLAKTFKSGDHILIDLEESLDADGDKVSKIVLKAEKPETEEEMPPALIGA"
BASE COUNT          284 a          355 c          340 g          324 t
ORIGIN      
        1 tgatgccagt cgatttggct aattcccgca agcgagccgt gcactcgcgc acctgagaaa
       61 tcgagccggc ggtcgactcc atctctggga gagtcatcgt ttggattgaa tccaccacca
      121 gaagcgccgg ctggacagtt tgaacatgcg taaagatttg tgagaggtta gtttcggtta
      181 cgaggtaaag tttttcaggg aggtgggcct ttccagcgcc atcacgcggc gccaagcggt
      241 cagcgcgcat cttgatttga cgttcagact cttccccaga gacatataaa accgtgtttt
      301 cttcggctaa ttgcattgcc atctgcagcg tgagcgtaga tttgccgata cctggatcgc
      361 cgccaatcag gacgatcgag ccgggcacaa tccctccgcc cagaacacgc gcgaattctt
      421 ccatcgccac aggcaggcga tcttccgctt gcccgtcgat ttcactgagg cggcgtggct
      481 gagaagacgc attcagcccg cgtacagtgc gggctgaatg actactttcg ggttcgacca
      541 cctcttcgac cattgaatcc cagctatcac aggctgggca gcgaccgaag ggctgtgaag
      601 ttacctttcc acaaacctga cagacatagc ggctatatgc tttagccata gtgtccttag
      661 gctcctatca gagccggcgg catttcttct tcggtttctg gtttttcagc tttgagcaca
      721 atcttactta ccttatcgcc atcggcatcc aagctttcct ccaggtcgat gaggatatga
      781 tcgccagact taaaggtttt cgccagcaag gcatcggcta aaggttcttc caccatttgc
      841 tcgattacgc ggcgaaccgg ccgagcaccc atttcagggt caaagccttc attggctaag
      901 taatccaacg cagcttcggt ggtagtcagc gtcaggtcgt aatcggagag gcggtcagca
      961 accttctgca gctcgaggtt gacaatcttg cggatatcct cttggttgag cgcacggaag
     1021 acgacgaccg agtctaaacg gttgatgaat tccggtcgga aagtgcgttt gagggcgtcc
     1081 atcagctttt tgcgcatttc ctcgtagctg gattgctcct cgagcgcctg atccacttcc
     1141 agtgaaaagc ccatggaagc ttgccgctta atcaaatcag cgccgatgtt tgaggtcatg
     1201 atgataatcg cattgcgaaa atcaacctta tgccccttgg catcggttag gtgcccttct
     1261 tccataattt gcagcaagag ctgcaaggct tcagggtgtg cct
//
LOCUS       DAOTLW010000162         1289 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_85966_length_1289_cov_12.384117, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000162 DAOTLW010000000
VERSION     DAOTLW010000162.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1289)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1289
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_85966_length_1289_cov_12.384117"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(277..1278)
                     /locus_tag="P5516_09535"
     CDS             complement(277..1278)
                     /locus_tag="P5516_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011805635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1595-like element ISCsp2 family transposase"
                     /protein_id="HRT92708.1"
                     /translation="METQMLTPTPGRDYPRTWNEFLDWFATEEACQAFLEKLRWPQGF
                     VCPRCGNAGDVYRASRTRLMCRSCQYQGTVTSGTIFDKTRTPLRVWLAAAWYLTNQKQ
                     GVSALGLQRVLGLGSYQTAWTMLHRFRRAMVRPGRDKLKGLVEVDETYLSITDRKNPA
                     TPAGRKSSTTKVLMVMAVEIVEPKGFGRIRLRRIDRDAATHVIPFVQEVVEPGAQVRT
                     DGSAAYRALGELGYTHQRTVMLGSGVPAHVSMAGVHRVASLVQRWVLGTHHGSVQPDH
                     LDAYLDEFVFRFNRRTSSSRGMLFYRLLQQAVVTPPVTYGDVVSKNTGESQSDTIAG"
BASE COUNT          256 a          382 c          382 g          269 t
ORIGIN      
        1 gctacgcggt tgttgcgggg agcattgcga cgtgaccgga aggcggctgg gcagtgagaa
       61 cctgttcaaa gacggccatg gcattagaaa gagcctgagc gcatccattt gaagcctttt
      121 tggcccccag cgcttatgta acaagcgcaa gcagctatca attcaggagt taaagcctgt
      181 actggatttt ccccaggctg gcgtcaggca ttgcattcac tgtaaattca taaggggtat
      241 ctatttagct ccagcagccc cattgatact gtgatttcat ccagcaatgg tatccgattg
      301 gctctccccg gtgttcttgc tcacgacatc cccatacgtc actggcggcg taaccaccgc
      361 ttgctgcagc aagcggtaaa acagcatccc gcgtgagctg gatgtgcgcc ggttgaaacg
      421 gaacacgaat tcatcgagat aggcgtccag gtggtctggc tgtacagagc catgatgtgt
      481 tcccagcacc cagcgctgta ccagtgaggc gacccggtgc actcccgcca tggagacatg
      541 ggcaggtacg cctgagccga gcatgacggt gcgctggtgg gtgtaaccca gttctcccag
      601 agcgcggtat gccgccgaac catccgtgcg tacctgggct ccaggctcga ccacctcctg
      661 cacaaagggg attacgtggg tggcggcgtc tcggtcaatg cggcgtaacc ggatgcgccc
      721 aaaccccttg ggctccacga tctccaccgc catgaccatc aacactttgg tggtgctgct
      781 cttgcgccct gcaggggtgg cgggattctt gcgatccgtg atggacaggt acgtttcatc
      841 cacctccaca agccccttga gcttgtcccg gcccgggcgg accatggcac gtcgaaaccg
      901 gtgcagcatg gtccaggcgg tctggtagct ccccaaaccc aatacgcgct gcagccccag
      961 ggcgctcacg ccttgtttct gattggtcag gtaccaagct gcagccagcc agacacgcag
     1021 cggtgtgcgc gtcttgtcaa agatggttcc agatgtcact gtgccttggt actggcatga
     1081 gcggcacatc aggcgggtgc ggctggcgcg gtacacatcg ccagcgttgc cgcaacgcgg
     1141 gcagacgaag ccttggggcc accgaagctt ctccaggaac gcctggcaag cctcttcggt
     1201 ggcaaaccag tcaagaaatt cgttccaggt gcgggggtag tcccgtccag gagttggcgt
     1261 tagcatctgg gtttccatcc agctattct
//
LOCUS       DAOTLW010000163         1287 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_86193_length_1287_cov_9.370942, whole genome shotgun sequence.
ACCESSION   DAOTLW010000163 DAOTLW010000000
VERSION     DAOTLW010000163.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1287)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1287
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_86193_length_1287_cov_9.370942"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="P5516_09540"
     CDS             <1..368
                     /locus_tag="P5516_09540"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HRT92709.1"
                     /translation="TPDTLIKDLKADELFALVLPGGWAPDKLRRYAEVKDLVAKVNSQ
                     NKIIGIICHGGLIAISAGIVRGKHAIGSLGIKDDLENAGAIWDDRPAFREGNLVWGRV
                     VADIPDFNRELVAALEERA"
     gene            432..>1287
                     /gene="pdxS"
                     /locus_tag="P5516_09545"
     CDS             432..>1287
                     /gene="pdxS"
                     /locus_tag="P5516_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRT92710.1"
                     /translation="MEQQVSSFAVKKGLAQMLKGGVIMDVVTPEHARIAEDAGAVAVM
                     ALERVPADIRASGGVARMSDPELILKIMETVSIPVMAKCRIGHFVEAQILEALGVDYI
                     DESEVLTPADEANHIYKHAFKVPFVCGCRNLGEALRRIGEGAAMIRTKGEAGTGDVVE
                     AVRHARQVFSEIRILQAMPEEEVMAFAKNIGAPYELCLEVRELGRLPVVNFAAGGIAT
                     PADAALMMQIGVDGVFVGSGIFKSGDPAKRAAAIVKATTHYNEPKLLAEISRNLGEPM
                     VGRQVSNIP"
BASE COUNT          295 a          304 c          387 g          301 t
ORIGIN      
        1 ttaccccgga taccttgatc aaggacctga aagctgacga gctctttgcg ctggtgctgc
       61 caggcggctg ggcgccggat aaactgcgcc gttatgctga ggtcaaggac ctggtagcca
      121 aagtgaattc acaaaataaa attattggca ttatttgcca tggcgggtta atcgccattt
      181 cggctggcat tgtgcgcgga aagcacgcca tcggttcgct cggaattaag gacgacttgg
      241 aaaacgccgg cgccatttgg gatgaccgcc ctgctttccg tgagggtaac ctggtgtggg
      301 ggcgggtggt tgcagatatt ccagatttca accgtgagct tgtggctgcc ttggaggagc
      361 gtgcataaag cttctttaca ttaatgcagt ggtacaatgc aagtgagaat atttaagttt
      421 ttggaggttg catggagcag caagtttcaa gttttgctgt gaaaaagggg ttagcacaaa
      481 tgttaaaagg cggcgtcatt atggatgtgg tgacaccgga gcatgcccgc attgcggagg
      541 atgccggcgc agtggcggtg atggcgctcg aacgcgtccc agcggatatc cgtgccagcg
      601 gcggcgtagc ccgcatgagt gaccctgaac tgatccttaa aattatggag acagtcagca
      661 ttccggtgat ggctaagtgc cgcattgggc attttgttga agcacaaata ctggaagctt
      721 taggcgtgga ttacatcgac gaatccgagg tcctgacccc agctgatgaa gctaatcata
      781 tttacaaaca tgccttcaag gtaccgtttg tgtgtggctg ccgcaattta ggtgaagctt
      841 tgcgccgcat tggcgaaggc gcggcaatga tccgcaccaa gggcgaagcc ggcacaggag
      901 acgtggtgga agccgtacgg cacgcccgcc aggtcttcag cgagattcgc atcctgcagg
      961 ctatgcccga agaagaagtg atggcatttg ctaaaaacat tggtgctcct tacgagctgt
     1021 gcttggaagt gcgcgagctt ggcaggctgc cggtggttaa ttttgccgcg ggcggcattg
     1081 cgaccccagc cgatgcagct ttgatgatgc aaatcggtgt tgatggcgtt tttgtcggct
     1141 cgggcatctt taaatctggc gatccagcca aacgtgccgc agccatcgtc aaggcgacca
     1201 cgcattataa cgaacccaag ttactcgctg aaatcagccg caacttaggc gagcccatgg
     1261 tagggcgcca agtttccaat attcctg
//
LOCUS       DAOTLW010000164         1258 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_89276_length_1258_cov_4.353283, whole genome shotgun sequence.
ACCESSION   DAOTLW010000164 DAOTLW010000000
VERSION     DAOTLW010000164.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1258)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1258
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89276_length_1258_cov_4.353283"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="P5516_09550"
     CDS             complement(<1..621)
                     /locus_tag="P5516_09550"
                     /inference="COORDINATES: protein motif:HMM:NF024907.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integrin alpha"
                     /protein_id="HRT92711.1"
                     /translation="MRKPKLFTIVLMAGLLLLPLFSSSAQSEINSSTEMLPPNTPGWT
                     FTGEQSLNYLGQSWMPAGDVNGDRYDDLIVGAYGYDTPTITNAGRAYLFYGYYTGLDT
                     TPDWVVDGEMTDAYFGYSVAGAGDVNGDGFDDVLVGSYGYDLAEPETVNVGKVYLYLG
                     SEDGLETTPAWTFTGSQEGQAVGVKVAGLGDVNSDGFDDIAIGSSGW"
     gene            931..>1258
                     /locus_tag="P5516_09555"
     CDS             931..>1258
                     /locus_tag="P5516_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92712.1"
                     /translation="MDPKSLFLLEYPKILTKLKAYAAFSASEALAGSLRPTANLEKAR
                     TLQQQTREARHLLSLSDSISFQGAEDLRPLVERARHGAALEASELLALRNTLVISREA
                     RRILEAH"
BASE COUNT          303 a          335 c          300 g          320 t
ORIGIN      
        1 ccatccgctt gaaccaatgg cgatatcatc aaaaccatcg ctattcacat ctcccaagcc
       61 ggcaactttt acaccgacag cctggccttc ctggctgcct gtaaaggtcc aggcgggtgt
      121 ggtttcgaga ccatcttcgg aacccaggta taagtagact ttaccgacgt ttacggtttc
      181 aggttcagcc aaatcgtaac cgtaactgcc gaccaggaca tcgtcaaatc catctccgtt
      241 cacatctcct gccccagcca cgctgtaccc gaagtatgca tccgtcattt ctccgtcaac
      301 tacccaatcc ggcgtggtat ccagcccggt gtaatagcca taaaacaggt aggcccgacc
      361 tgcatttgtt atggttggag tatcataccc atacgcgcct acaatcaggt cgtcataccg
      421 atcgccattg acatcacccg ctggcatcca ggattgaccg agatagttca atgactgttc
      481 gccagtgaag gtccagccag gggtgtttgg cggcaacatc tctgttgatg aatttatttc
      541 gctttgtgcg gaactggaga acaagggcag gagcaataac cctgccatta atacaattgt
      601 gaaaagtttt ggtttgcgca tcggtgatcc tccttctgaa acatgtcttg ttgattaggt
      661 ggtggtcaca aggctatccc gatatctacg aaaatattga cgggagttag cctttgatct
      721 ttgccacggc ttgggttgtg cgtgccttat tcagatgtac gagattgttt agacgactgc
      781 aagggtttgt gcacctttgg gaagatgctg ccctgatttt tatagtataa aggattttat
      841 tagcaaatac aataccattt cacccatttt aagattataa aaatcacacg taatccagga
      901 atgggcaatc atgataaact ttcttgacca atggacccca aaagtttgtt tctgctggaa
      961 tatcccaaga tcttgacgaa gctgaaggct tatgctgctt tcagcgcctc cgaggcattg
     1021 gctggctcct tgcgcccgac ggcgaactta gaaaaagcgc gcactctgca gcagcaaacg
     1081 cgcgaagccc gccacttgct ttcactcagt gattccattt cctttcaggg tgcagaagac
     1141 ctgcgcccgc tggtggagcg cgcccgccat ggtgcagcgc tggaagccag cgaactgctc
     1201 gcgctgcgca acacactggt gatttcgcgg gaagcccgcc gcatcctgga agcgcatg
//
LOCUS       DAOTLW010000165         1255 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_89579_length_1255_cov_11.095833, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000165 DAOTLW010000000
VERSION     DAOTLW010000165.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1255)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1255
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_89579_length_1255_cov_11.095833"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1255
                     /locus_tag="P5516_09560"
     CDS             <1..>1255
                     /locus_tag="P5516_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
                     /protein_id="HRT92713.1"
                     /translation="LGKTLGIGALAVCALVFIVGVTRLVLSTAEFSLSDPFIIQELLN
                     LFMIAVSLAIAAVPEGLPAVVTISLAGGMREMVKRHALIRKLASVETLGSVTVICSDK
                     TGTLTQNQMTVTRLWADGKFITVTGSGYEPHGEFLNDDHKIDFKSYRGVGTALWIGAL
                     NNDAQLELSGESDGKPTYRIVGDPTEGSILVAAEKAGTPVEDLQKAYPRVQEIPFDSE
                     RKRMLTIHAIKQPVDDDISPFTSADRGKGYIIAVKGAPDVVLNLCTQRLKLDNSVEPL
                     TEEGRKEILAANDAMTKDALRVLGVAYRVVPQMPEEITPEALEKDLIFAGLIGMIDPA
                     RPEVKAALTEAKTAGIRTIMITGDYPNTARAIAEEIGLIAPGHQVLTGADIEDKSQEE
                     MREIVKITDVYARVSPEHKMKIVDAL"
BASE COUNT          311 a          332 c          326 g          286 t
ORIGIN      
        1 ctcggaaaaa cgctcggcat tggcgcctta gcggtgtgtg cattggtctt cattgttggc
       61 gttacgcggt tggtactttc aacggctgag ttttcgttaa gtgatccgtt tattatccag
      121 gagctgctca acctcttcat gatcgcggtc tcccttgcca tcgcggctgt acccgaaggc
      181 ttacctgccg tcgtgaccat cagcttagct ggcggaatgc gtgaaatggt caagcgtcat
      241 gcgctcatcc gcaagttagc ctcggtggag actttaggct ccgtgaccgt gatttgctcg
      301 gataaaactg gaaccttgac gcaaaaccag atgaccgtca cgcgcctttg ggcagatgga
      361 aaatttatta ccgtcaccgg aagcggttat gagccacacg gtgaattttt gaatgatgat
      421 cataagattg acttcaaatc ttatcgcgga gtaggaactg ctttatggat tggtgcgctg
      481 aataacgacg cccagcttga gctcagtggt gaaagcgatg gcaagcccac ctaccgcatc
      541 gtgggcgatc cgaccgaagg ctctatttta gtagccgccg aaaaagcggg taccccagtc
      601 gaagacctgc aaaaagctta cccacgcgtg caggagatcc cattcgactc cgagcgtaag
      661 cgcatgctca ccattcatgc aattaagcag cctgtggatg atgatatttc tcctttcacc
      721 tcggctgacc gtggaaaagg ttatatcatc gcggtcaaag gagccccaga tgttgtgctc
      781 aacctttgca cgcaacgcct gaagttagat aattcggttg aacctctcac cgaagagggc
      841 cgcaaagaga ttttagctgc caatgatgcg atgactaaag acgcactgcg tgtccttggc
      901 gtcgcttatc gcgtggttcc gcagatgccc gaagaaatta ccccagaagc actcgagaaa
      961 gatctcatct ttgcaggtct catcggtatg attgaccctg cccgaccaga agtgaaagca
     1021 gccttaaccg aagccaagac tgcaggcatt cgcaccatca tgatcaccgg agactacccc
     1081 aacaccgcac gtgccatcgc cgaggaaatc ggattgatcg cccccgggca ccaggtgctc
     1141 accggagccg atattgagga caaatcccag gaggagatgc gcgaaatcgt caaaatcaca
     1201 gatgtttacg cgcgcgtgag ccctgaacat aagatgaaaa tcgtggatgc cttgc
//
LOCUS       DAOTLW010000166         1230 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_92403_length_1230_cov_10.365106, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000166 DAOTLW010000000
VERSION     DAOTLW010000166.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1230)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1230
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_92403_length_1230_cov_10.365106"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1230
                     /locus_tag="P5516_09565"
     CDS             <1..>1230
                     /locus_tag="P5516_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HRT92714.1"
                     /translation="VDHPDIEDFITCKHNEYAITNFNISVAITDAFMRAVEADEPWEL
                     RFPDTQSPLYKTFSGTLEGAERAGIPIRTYKTVPARELFNKLVAQAHHNGEPGALFLD
                     AANRANPVPHLYDLEATNPCGEQWLGPYENCCLASINLAEHIGPDGSVDWAKLEQTTQ
                     TVTRFLDDVIDKNAYVPAVPQLKESALTARRIGLGIMGLADLMYHCGVRYGSDKSLEF
                     ASQVMEFIRYHCMLTSIHLAAERGPFPAIQGSIYDPEALKWTPPEPLIPYTNDWQRPE
                     VNWQQVVDGIKRHGIRNAAQTTIAPTGTIGTVAGCEGYGCEPVFALAYVRHVNDHGKD
                     LQLNYVSPLFQAALDRTDLSPEIKAEIITQVIEQGTCQNIAELPEEIRDVFVVSADVT
                     AREHIRIQAALQRFVDN"
BASE COUNT          302 a          336 c          302 g          290 t
ORIGIN      
        1 gggtggatca ccctgatatc gaagacttta tcacctgtaa acataatgaa tatgccatta
       61 ctaatttcaa tatttccgtc gccatcactg atgcgtttat gcgcgctgta gaagccgatg
      121 aaccctggga actgcgcttt ccggatactc aatccccgct ctataaaacc tttagtggca
      181 ccttagaggg cgcagagcgc gctggcattc cgatccgcac ctataaaaca gtacctgccc
      241 gtgagctttt caataaactc gttgcgcagg cacaccacaa cggcgaacct ggtgccttat
      301 tcctggatgc agccaaccgc gctaaccccg tcccgcactt gtatgacttg gaagccacga
      361 acccctgtgg cgagcaatgg ttaggaccgt atgaaaactg ttgtttagcc tccattaatt
      421 tagccgaaca cattggacca gatggatccg ttgattgggc aaaattagag caaactacac
      481 agacggtaac ccgctttctc gatgacgtca ttgataaaaa tgcttacgtg cctgccgttc
      541 cgcaactgaa agagtctgca ttaacagccc gccgtattgg gctggggatc atgggcttag
      601 ccgacctgat gtatcactgt ggggtgcgct atggctcgga taaatccctc gagtttgcct
      661 cacaggtgat ggagttcatc cgttaccatt gcatgctcac gagtattcac ttagccgccg
      721 agcgcggtcc cttcccggct attcaaggca gcatttatga cccggaagcg ctgaaatgga
      781 ctccaccaga gccattaatt ccctatacaa acgattggca gcgccccgaa gttaactggc
      841 agcaggtcgt cgatggcatc aagcgccatg gtatccgcaa cgctgcccag acaaccatcg
      901 cacctactgg tactattggc acagtggctg ggtgcgaagg ctatggctgc gaaccggtgt
      961 ttgctttggc ttacgtgcgc catgtaaatg accatggcaa ggatctgcaa ttgaattacg
     1021 tcagcccgct cttccaagca gcgcttgacc gcactgacct cagcccagag attaaggctg
     1081 aaatcattac ccaggtgatt gagcagggca cctgccagaa tatcgctgag ctgcccgaag
     1141 aaatccgcga tgtatttgtg gtttcagcgg atgtaacggc acgtgaacac atccgcattc
     1201 aagcagcgtt gcagcgcttt gttgataaca
//
LOCUS       DAOTLW010000167         1207 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_95142_length_1207_cov_8.210938, whole genome shotgun sequence.
ACCESSION   DAOTLW010000167 DAOTLW010000000
VERSION     DAOTLW010000167.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1207)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1207
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95142_length_1207_cov_8.210938"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..897
                     /locus_tag="P5516_09570"
     CDS             <1..897
                     /locus_tag="P5516_09570"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HRT92715.1"
                     /translation="ADVLKMLDDIAEKERLEYAKRDPVDAEPQIDRAAELNEKWQVVT
                     GDLWQIGGHRLLCGDSTKREDVERVMGGEKADMCFTSPPYNAGISAQLSGNTSIDDNL
                     YRDEYDDNKTQTDYFDLLVGFTNNALEYSKYVFVNIQVLSGNKKAFIDYWSNFSDKFC
                     DVAIWDKCHAAPALAQRVMDSRFEFVLVFGGNGSRAIGTRDFRGMVHNVYSGNPQRHN
                     DNASVHAATFPIDFPEYFINNFTNKDEIVLDPFLGTGTTMVACENLHRKCRGIEISPN
                     YCAVILERMATAFPELEIKKVD"
     gene            904..>1207
                     /locus_tag="P5516_09575"
     CDS             904..>1207
                     /locus_tag="P5516_09575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92716.1"
                     /translation="MGHRRLTKKIVREALINKKGAVYLAASDLGCSHTAIYDYLDKYP
                     DLQELKDGFDEEVTDIAVLNLRKAVINADPWALKYQLSTKGKNRGYVERQEVTGADG"
BASE COUNT          316 a          258 c          342 g          291 t
ORIGIN      
        1 gcggacgtgc tgaaaatgct ggatgacatt gcggagaagg aacggctgga gtacgccaag
       61 cgtgaccccg ttgacgctga accgcagatt gaccgcgccg ctgaactcaa cgagaagtgg
      121 caggttgtca cgggtgacct gtggcagatt ggcgggcatc ggctgttatg cggcgacagc
      181 acgaagcgtg aggacgtgga gcgggtgatg gggggtgaga aggcggatat gtgttttact
      241 tcaccaccat acaatgctgg tatttccgcg caacttagcg gaaataccag cattgatgac
      301 aacctttatc gtgacgagta cgatgacaat aaaacacaaa cggactattt cgatttattg
      361 gtaggtttta cgaacaatgc gcttgaatat agcaaatatg tgttcgtgaa tattcaagtt
      421 ttatctggca ataagaaagc atttatagat tattggagca acttctcgga taagttttgt
      481 gacgtggcaa tatgggataa atgccacgct gcgccagctc tggcgcagcg tgtaatggat
      541 tcacgttttg agtttgtttt ggtatttggt ggcaacggtt cacgcgcgat tggtactcgt
      601 gatttcaggg gcatggttca caatgtttat tctggcaatc cgcagcgaca caacgacaat
      661 gcaagcgttc acgcggccac gtttccgatt gattttcccg aatattttat taataacttc
      721 acgaataaag acgaaattgt acttgacccg tttttaggaa ccggcacgac aatggtagcc
      781 tgcgagaacc tgcaccgcaa atgcagaggg attgagatat caccaaacta ctgcgccgtg
      841 atactggagc ggatggcgac cgcgttccct gaactggaaa tcaagaaagt tgactaactt
      901 tacatgggac atcgtaggct gaccaagaag atcgtgaggg aggcgctcat caacaagaag
      961 ggcgcggtct atttagccgc ctctgacttg ggatgttcgc acaccgctat ttacgattat
     1021 cttgacaagt accccgattt gcaggaactc aaggatggct ttgacgagga agttaccgac
     1081 atcgccgtgc tgaatttacg caaggcggtg atcaatgctg acccgtgggc gctgaagtat
     1141 caactatcca cgaagggcaa gaatcgcggt tacgttgagc ggcaggaggt cacgggcgcg
     1201 gatggtg
//
LOCUS       DAOTLW010000168         1203 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_95632_length_1203_cov_9.936411, whole genome shotgun sequence.
ACCESSION   DAOTLW010000168 DAOTLW010000000
VERSION     DAOTLW010000168.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1203)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1203
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_95632_length_1203_cov_9.936411"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1203
                     /locus_tag="P5516_09580"
     CDS             <1..>1203
                     /locus_tag="P5516_09580"
                     /inference="COORDINATES: protein motif:HMM:NF013704.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA modification methylase"
                     /protein_id="HRT92717.1"
                     /translation="GKFANRIVGSGEESLDQIQFNPRNWRVHPLNQQNALKGVLEEVG
                     WVQEVIVNKRTGNLIDGHLRCQLAAREGAKTIPVKYVDLSEDEEALVLSTLDPIAAMA
                     VTDKQKLDDLFASIETENADVLKMLDDIAEKERLEYAKRDPVDAEPQIDRAAELNEKW
                     QVVTGDLWQIGEHRLLCGDSTKREDVERVTGGENAVLMLTDPPYGVDYGELVRSRVNQ
                     KKDGWNDIENDALSDDDLYTLLLNSLSGCGAVTGFVWHPPGARRWLFWKAVEENGWRV
                     SQEIVWVKNSMVFGRSDYQWRHEPCLYLKREGSKKQEDRTQTTVWEVDKPTNSIHPTM
                     KPVELFEIAVRNHTQEGDITYEPFCGSGTTMVACQNLNRKCRAIEISPNYCAVILERM
                     STAFPELE"
BASE COUNT          319 a          258 c          360 g          266 t
ORIGIN      
        1 tgggtaaatt cgcaaaccgc atcgttggct caggcgagga atcgcttgac cagatccaat
       61 tcaacccgcg taactggcgg gtgcatccgc ttaaccaaca gaacgctctc aagggcgtgc
      121 tggaagaggt ggggtgggtt caggaagtca ttgtcaacaa gcgcaccggg aacctgattg
      181 acgggcattt gcgctgtcaa ttggcggcaa gagaaggggc aaagacaata ccagtcaagt
      241 atgtggactt gtcagaagac gaggaggcgc tggtactcag cacccttgat cctatcgccg
      301 caatggctgt caccgataag cagaagttgg atgacttgtt cgccagcatc gaaacagaga
      361 acgcggacgt gctgaaaatg ctggatgaca ttgcagagaa ggaacgtctg gagtatgcga
      421 agcgtgaccc cgttgacgct gaaccgcaga ttgaccgcgc tgctgaactc aacgagaagt
      481 ggcaggttgt cacgggtgac ctgtggcaga ttggcgagca tcggctgtta tgcggcgaca
      541 gcacgaagcg tgaggacgtg gagcgggtga cggggggtga aaatgcggtt ttgatgctaa
      601 ccgacccacc ctatggcgtt gattatgggg aattggttag aagtcgtgtc aaccaaaaga
      661 aagatggctg gaacgatatt gagaatgacg cgttgagtga tgatgattta tatacgttgc
      721 tacttaactc actcagtggc tgtggtgctg tgactgggtt cgtctggcac ccgccgggtg
      781 ccagacggtg gttgttctgg aaagccgttg aagaaaatgg atggcgtgtt tctcaagaga
      841 ttgtatgggt aaaaaactca atggtgtttg gccgttcaga ttatcaatgg cgacacgaac
      901 cttgtttata tctgaaacga gaaggctcta aaaaacaaga ggacagaacg cagacaactg
      961 tttgggaagt tgataaaccg accaattcta ttcatcccac tatgaagcca gtcgagttat
     1021 ttgaaatagc ggttcgtaat catacgcaag aaggcgacat tacttatgag cctttctgtg
     1081 gctccggcac aacgatggtg gcgtgtcaga acctgaaccg gaagtgccgc gccattgaga
     1141 tttcaccaaa ctactgcgcg gtaatccttg agcggatgag caccgcgttc ccagagttgg
     1201 aaa
//
LOCUS       DAOTLW010000169         1193 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_96842_length_1193_cov_19.413884, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000169 DAOTLW010000000
VERSION     DAOTLW010000169.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1193)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_96842_length_1193_cov_19.413884"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1115)
                     /gene="lpdA"
                     /locus_tag="P5516_09585"
     CDS             complement(<1..1115)
                     /gene="lpdA"
                     /locus_tag="P5516_09585"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HRT92718.1"
                     /translation="MDNFDVVVIGSGPGGYVSAIRAAQLGLKVAIVERKALGGVCLNI
                     GCIPSKALLRNAELVYLMRTQAAEFGIHAENVTFDYNAAYKRSRQVSERLTKGVSFLM
                     KKNNIAVIEGHGEVANPGEVLVRAADGGTQTISTRNIVLATGAHPTIIPGMEPDGEKI
                     LDYEDAILLQQLPKSAVIIGAGAVGVEFATVWSAYGVEVHIVEMLPHILPAEDDEAAA
                     ELSKAFRKRGVKIHAGTKVQSVQKTENGVEVQIAGEKGEEKITAEAVLVAVGFKPNSS
                     NIGLEKVGVAVDQRGFVQIDDSMATNIPGIWAIGDVTGKLLLAHTASTMGIICAEVIA
                     GLPARKLDYRMIPRATYTQPQVASFGYTEKQAQEAGY"
BASE COUNT          262 a          356 c          267 g          308 t
ORIGIN      
        1 ttataaccag cttcctgagc ctgcttttcg gtatatccaa aagaagctac ctggggttgt
       61 gtatacgttg cgcgtggaat catgcggtaa tccaacttgc gcgcaggcag cccggcgatg
      121 acttccgcac agataatgcc catggttgag gcggtgtgcg ccaggagcag cttgccagtc
      181 acatccccga tcgcccaaat gccaggaatg ttggtagcca tgctgtcgtc aatctgcaca
      241 aaaccgcgct ggtcaaccgc tacacccact ttttccagcc cgatattgct tgaattgggt
      301 ttgaacccaa cagctacgag cacggcttca gcggtgattt tttcctcacc cttttctcca
      361 gcaatctgca cttccacgcc gttttcggtc ttttgcacag attgcacttt ggtgccagcg
      421 tggatcttca cgccgcgttt cctgaaagct ttgctaagct cggctgctgc ttcatcgtct
      481 tccgccggca agatatgcgg cagcatctcg acgatatgta cctccacgcc ataagcgctc
      541 cagactgtcg caaactccac acccactgca ccagcgccaa taatcaccgc cgactttggc
      601 agctgctgca gcaggatggc atcctcataa tccaaaatct tttcgccatc cggctccata
      661 cctgggatta tcgtggggtg tgcgccggta gctaaaacta tgttccgtgt ggagattgtt
      721 tgtgtccctc catcagccgc cctgaccagc acttccccgg ggttggcaac ctcgccatgc
      781 ccctcaataa cagcgatatt gttctttttc atgagaaaac tcacaccctt cgtcaggcgc
      841 tcagagacct gccggctgcg tttgtaggct gcgttatagt caaaggtcac attctcagca
      901 tgaatgccaa actccgctgc ttgtgtgcgc atcaagtaga ctaactcggc attccgcaga
      961 agtgctttcg agggaatgca gccaatattg aggcagaccc cgcctaaggc ttttcgttcc
     1021 acgatagcaa ccttcaagcc cagctgtgcc gcccgtattg cgctgacgta gccgccaggt
     1081 ccgctgccaa tcaccacaac gtcaaagtta tccataaatt gctccttatt ttcatttacg
     1141 ttaggcttaa gtaaatcaca attcctgtga atatcaaatt taatccttat tct
//
LOCUS       DAOTLW010000170         1188 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_97519_length_1188_cov_10.278906, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000170 DAOTLW010000000
VERSION     DAOTLW010000170.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1188)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1188
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97519_length_1188_cov_10.278906"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            75..>1188
                     /locus_tag="P5516_09590"
     CDS             75..>1188
                     /locus_tag="P5516_09590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92719.1"
                     /translation="MTGITDEALVAALNELSLKGFISANLEDRTEGSYVFSREVERGI
                     ILDQLGPAEKRRLHLRAAKALLSRRPRQPAFAKEIAFHFETAGETQPAVKAWLEAGRY
                     ARSKYSKDETYLSYGRALDLISRLPAGYDESVIYQAANEWGNYAHDQDDSRTCEKIYQ
                     RCLEVGEATHSLLLIGTGLSGLGRAADFLYEYEKSTDYFQRAIFYLSNTGYDAELIKA
                     LSRLGIMQFGRDEYTRAYELLTDALKRDPATKDQDSLDNRVNILSYICILHIFFGEPG
                     KAEKIAAEMARLSVLVKRRSARVQGHALLAMAQYYNGRVQEALLTFRVNHPMAETLQV
                     RFWLSLLELVAAMAYLHNGDLDKSWYFIDRVHRREAN"
BASE COUNT          298 a          273 c          340 g          277 t
ORIGIN      
        1 gtcacgctca ttcgttcagt ttgacctatt ttacctattt ttgagatgac ttctacatag
       61 atgtcgttga agagatgacc ggcataactg acgaagccct ggtggcggct ttgaatgagt
      121 tgagcctgaa aggctttata agcgccaatc ttgaggacag gacggaagga agttatgtgt
      181 ttagccgcga ggtggaacgc ggaataatcc tggaccaatt gggccctgca gaaaagaggc
      241 gcctgcattt acgggcggcg aaagcgctgc tcagtcgccg tcccaggcag cctgcgtttg
      301 caaaagaaat cgcctttcat tttgaaacgg ctggagagac acagccggct gtgaaggcgt
      361 ggttggaagc aggaaggtac gcgcgttcaa agtattcaaa agacgaaacc tatctttcat
      421 atggccgggc tttggatttg atcagcaggt taccggcagg atatgacgag tctgtcattt
      481 accaggcggc gaatgagtgg gggaactatg cccacgacca ggatgattcg cgcacctgcg
      541 aaaaaattta tcagcgctgc ctggaggttg gagaagcgac gcacagcctg ctgcttattg
      601 gtaccgggct aagcggtttg ggtagagcgg cagatttctt gtatgaatat gaaaaatcaa
      661 cggattattt tcagcgggcc atcttttacc tgtccaacac cgggtacgac gcggagctca
      721 tcaaagcttt atctcgattg gggattatgc agtttgggag ggatgaatac acacgagcat
      781 atgaactgct cacggacgcc ttgaaacgcg atcccgcaac gaaagaccag gattcgcttg
      841 ataaccgcgt gaacatcctt tcttacatct gtatcttgca cattttcttt ggagagcctg
      901 gcaaggctga aaaaatcgcc gccgagatgg ccaggcttag cgtgctggta aaaagacgtt
      961 cagccagggt gcagggtcac gcgctcctgg caatggcgca atattataac gggcgcgttc
     1021 aagaagccct gctaaccttc cgcgtgaatc acccaatggc agagaccctg caggtgcggt
     1081 tctggctctc gctgttggaa ctggtggcag caatggcgta cctgcacaat ggagacctgg
     1141 ataagagctg gtattttatc gaccgggtgc atcgcagaga agccaatt
//
LOCUS       DAOTLW010000171         1185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_97910_length_1185_cov_5.619469, whole genome shotgun sequence.
ACCESSION   DAOTLW010000171 DAOTLW010000000
VERSION     DAOTLW010000171.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97910_length_1185_cov_5.619469"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(429..782)
                     /locus_tag="P5516_09595"
     CDS             complement(429..782)
                     /locus_tag="P5516_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010512344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRT92720.1"
                     /translation="MVKIKRVYESPQESDGVRFLVERLWPRGMKKEELIIDSWVKDVA
                     PSTELRRWFGHDPLKWDEFQKRYLAELAGNASAWMPILEAAQKGEVTLLYSAHDTEHN
                     SAIVLKSFLDQRLSK"
BASE COUNT          258 a          330 c          299 g          298 t
ORIGIN      
        1 gtgatggtaa gtactggaat cttcttgcgt aattctgcga tccccaaggt ttacctggca
       61 gtggtgtaca ccgcgatcgg cggtgcgctc attcaggcca gtgtgaaata ttacacgcgc
      121 ttcttttctc ttatccacag gggagtgtcc tgatccaatt tccttaaaag atgggcttgg
      181 agacgaggga aacaaagacg gtgggttgga gccctgcgcc cctctcgccc gtgccgcgta
      241 tgggtcacgc gcattctttt caaccttgtt agtggcgttg ggggcagagc tgcggagcgc
      301 tggtgaccgc gcggccgcga cctttgcccg agaaaagaac gcgccctgct tgacgatgaa
      361 gggataccct tatcgccgcg gcagaaagcg cgcttgcccg cggttcgggt gcgctcttca
      421 aatgaaaatc acttgcttaa acgttgatct aaaaaagatt taagcacaat ggcgctgttg
      481 tgctcggtgt cgtgagcgct gtaaagcagg gtcacctcac ccttttgagc agcctcgagg
      541 atgggcatcc atgcgcttgc gttcccagcc aattcagcca ggtagcgctt ctggaactca
      601 tcccatttca gcgggtcatg cccaaaccag cgccgcaatt cagtactggg ggcgacgtct
      661 ttcacccagg aatcaattat caactcttct tttttcatcc cgcgcggcca caggcgttcc
      721 accagaaaac gaaccccgtc actttcctga gggctttcat aaactcgttt tatcttcacc
      781 atatttactc ctttcaatta cggctgacct ctttgccctg ccgacagacg cgcagctaat
      841 gaacatcact cacagcggtg acgtccaccc agcgggcgta cccattgcgt gttccattgt
      901 aattcaaatc tccgggcaaa tgctgccaat acttagagga caaaccccta tcagggcgcc
      961 agcactgagc ccatccagct ggtaaaggag atgcctctca ggtaaaggtg ttttttgatt
     1021 ccctctgagt cggaaaggac cacatattca tgcgtctggg cttttatcgt ttccggctgg
     1081 tgcgcgagtg gaaagaatat ccttttgatc ctttcgcgct ctattcgctg ccgggaaaat
     1141 cggtgatcac accatcgttg agcagaaccc catttacttt ataga
//
LOCUS       DAOTLW010000172         1185 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_97912_length_1185_cov_5.093805, whole genome shotgun sequence.
ACCESSION   DAOTLW010000172 DAOTLW010000000
VERSION     DAOTLW010000172.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1185)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1185
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_97912_length_1185_cov_5.093805"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(429..782)
                     /locus_tag="P5516_09600"
     CDS             complement(429..782)
                     /locus_tag="P5516_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HRT92721.1"
                     /translation="MVKIKRVYERPQESDGVRFLVERLWPRGMKKEELKIDSWVKDVA
                     PSTDLRRWFGHDPLKWDEFQKRYLAELAGNASAWMPILEAAQKGEVTLLYSAHDTEHN
                     SAIVLKSFLDQRLNK"
BASE COUNT          263 a          325 c          297 g          300 t
ORIGIN      
        1 gtgatggtaa gtactggaat cttcttgcgt aattctgcga tccccaaggt ttaccaggcg
       61 gtggtgtaca ccacgatcgg cggtgcgctc attcaagcca gtgtgaaata ttacacgcgc
      121 ttcttttctc ttaaccgcgg gggagggtcc tgatccgatc ccttcaaaaa acgggcttgg
      181 agatgagaga aacaaagacg gaggcttgga gccccgcgcc cctcttgccc gtgtagcgca
      241 tgggtcacgc gcattccttt ctgccttgac tgtaccgttg ggggcgggac tgcggagagc
      301 tggtgacccc gcgatcgcga cctttgccgg agaaaagaac gcgccttgtt tgacgatgaa
      361 gggacaccct taacgccgcg gcagaaagcg cgtttgcccg cggttcgggt gcgctcttca
      421 aatgaaattc acttgttcaa acgttgatct aaaaaagatt taagcacaat ggcgctgttg
      481 tgttcggtgt catgagcgct gtaaagcagg gtcacctcac ccttttgagc ggcctcgagg
      541 atgggcatcc atgcgcttgc gtttccagcc aactcagcca ggtagcgctt ctggaactca
      601 tcccatttca gcgggtcatg cccaaaccag cgccgcaaat cggtactggg ggcgacgtct
      661 ttcacccagg aatcaatttt cagctcttct ttcttcatcc cacgcggcca caggcgttcc
      721 actagaaaac gaaccccgtc actttcctga ggtctttcat aaactcgttt aatcttcacc
      781 atagttgctc ctttccatta aggctgacct ctttgcactg cagacagacg cgcagcgttt
      841 gaacatcacc cacagcggtg gcgtctaccc agcgcgctta cccattacgt tttccattgt
      901 aattcaaatc tccgggcaaa tgctgccaac acttagaggg caaatcccta tcagggcgcc
      961 ggcactgagt ccatgctgct ggtaaaggat tattctctca ggtaaaggtg ttttttgaat
     1021 acctctgggt cggaaaggac caaatattca agcgtttggg cttttatcgt ttcccgccag
     1081 tacgcgggtg gaaagaatat ccttttgatc ctttcgcgct ctattcgcta ccgggaaaat
     1141 cggtgatcac accatcgttg agcagaaccc catttacttt ataga
//
LOCUS       DAOTLW010000173         1184 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_98029_length_1184_cov_14.045173, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000173 DAOTLW010000000
VERSION     DAOTLW010000173.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1184)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1184
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98029_length_1184_cov_14.045173"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1157)
                     /locus_tag="P5516_09605"
     CDS             complement(<1..1157)
                     /locus_tag="P5516_09605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HRT92722.1"
                     /translation="MQQNKVLKTILFVILAIVVAAGLFMGGIVFGNQLPTGMVQSAFR
                     GQTATTQPTEVVTDVCTGYASTSTPEELKTLFAPFWESWQLLHENFVDQPLDDVALMR
                     GAIRGMLAATGDKHTSYMDPTEFQQANASMEGEYEGIGAWVDTTGDYLKIIAPMKGSP
                     AEAAGLKANDVVIAVDGEDQTGVPGDLVLQKILGPAGEPVTLTIRRGDETLDVTIVRA
                     KISVPVVDYQMLENEIAYIALTTFNDQATEQLRDALDELMAQNPKGLILDLRNNGGGY
                     LSTAIEVASEFIPEGVIMYEEYGDGTREVYNAKRGGRATEIPLVVLVNEGTASASEIV
                     AGAIQDHGRGKLVGTQTYGKGSVQNWIPLSTEDSGVRITIARWLTPNGNQI"
BASE COUNT          268 a          304 c          303 g          309 t
ORIGIN      
        1 ttgatttgat ttccatttgg agtgagccag cgtgcaatcg taatgcgtac gccgctatct
       61 tctgtggaga gcggaatcca gttttgcacc gaacccttac cataggtttg cgtgccaacc
      121 aacttacccc tgccgtggtc ctgaatggcg ccggcaacaa tttctgaagc ggatgcggtg
      181 ccttcgttca ccagaacgac taagggaatt tcggtggcgc gtccacctcg tttggcattg
      241 taaacttcgc gggtgccatc accatattct tcatacataa taacaccctc agggataaac
      301 tccgaagcga cttcaatagc agtagagagg taaccaccgc cattattgcg caggtcgagg
      361 atcaagcctt tggggttttg cgccatgagc tcatcgagag catcgcgaag ctgttcggtg
      421 gcttggtcgt taaaggtagt aagcgcgatg taagcaatct cgttttcaag catttggtaa
      481 tcaaccacag ggacactgat tttggcgcgc acgatggtta catccagagt ctcatcgcca
      541 cggcggatgg ttagggtaac tggctcgccg gctgggccta aaatcttctg cagcaccagg
      601 tcgccaggca cgcctgtttg gtcttcacca tccacagcaa taaccacatc attggctttc
      661 aagccagccg cttcggcagg tgagcctttc atcggggcaa ttatctttaa gtaatcccca
      721 gtggtatcca cccatgcgcc aatgccttcg tattcgcctt ccatgctggc attggcttgt
      781 tggaactctg ttggatccat gtatgaggta tgtttatcgc cagtggcggc gagcatgccg
      841 cggatggcac cacgcatcaa agcaacatca tccagaggct ggtcgacgaa attctcgtgc
      901 agcagctgcc agctttccca gaagggcgca aagagtgtct ttagctcttc cggcgtacta
      961 gtgctggcgt aacctgtgca gacgtctgtg accacttcgg taggttgagt tgttgccgtt
     1021 tgaccgcgaa aagcagattg caccataccg gtggggagtt ggtttccaaa aacgataccg
     1081 cccatgaata gtccagcggc tacgacgatt gccaatataa cgaataagat tgttttcagg
     1141 actttatttt gttgcataat tgctcccgtt tatttcttta gaat
//
LOCUS       DAOTLW010000174         1181 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_98423_length_1181_cov_5.538188, whole genome shotgun sequence.
ACCESSION   DAOTLW010000174 DAOTLW010000000
VERSION     DAOTLW010000174.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1181)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1181
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98423_length_1181_cov_5.538188"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..921)
                     /locus_tag="P5516_09610"
     CDS             complement(<1..921)
                     /locus_tag="P5516_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92723.1"
                     /translation="MMKRLMHYSITGIIVLALLLSGTLSAISPVEAAANPAPVQLYYV
                     TLPEIDGLRVLSGIHSAAIPPMYTYFSISIPVDGTFIYYDQWENGYALDIANPTPAEI
                     YSAGNIGGVQIWGNGRAADGCAPNINGVPFTCTDAADVLHAGDVIIPNNAIPLPPGPP
                     IYNVLDRFNTVAYNNQDGNANWTSNWVESGDNGNPASGQILITGNQLRFAVTDANDGI
                     DRGLDLSAAGECSTLTFTLGKSSTIGSNDRLQVRMSSGGSYNSLVSYDQNEPAGTKTV
                     NIPATYRTANARLQFRSQDALESGDYWTIDD"
BASE COUNT          287 a          310 c          303 g          281 t
ORIGIN      
        1 gtcatcgatc gtccagtagt cgcctgattc aagcgcatct tgcgatctga actgcagcct
       61 ggcgttcgcc gttcgatagg tggcgggtat attaacggtt ttagtacccg cgggttcatt
      121 ttggtcataa ctgacaagcg agttgtaact tccacccgac gacatccgta cctgtaatcg
      181 atcattactg cctatagtgg atgatttccc tagggtaaat gtcaaggtag agcattcccc
      241 ggctgcagaa aggtccaacc cgcggtcaat cccatcgttc gcatcagtaa ctgcaaagcg
      301 cagctggttg ccagtgatca ggatttgccc tgaagcggga ttcccgttat caccgctctc
      361 cacccagttg ctggtccagt tggcgttgcc gtcttgattg ttgtaggcaa ccgtgttgaa
      421 ccggtcaagc acgttgtaga tcggtggacc tggcgggagc ggaatggcgt tgttcgggat
      481 gatcacatct cccgcgtgta gcacatccgc cgcgtctgtg caggtaaaag gaacgccgtt
      541 aatgttcggc gcgcagccat ccgccgcacg cccgttgccc cagatctgca caccgccgat
      601 attgcccgcg ctgtaaatct ccgccggggt ggggttggcg atatccaacg cgtaaccgtt
      661 ctcccattga tcatagtaga tgaacgtgcc atccaccggg atcgaaatgg agaagtaggt
      721 gtacattggc ggtatcgcgg cactatgaat accgctcaat acgcgcaacc catcgatctc
      781 aggcagtgtc acataataca actgcaccgg cgctgggttg gccgccgcct caactggcga
      841 gatggcactg agcgtgcccg aaagtaataa tgccaggaca ataatgcctg tgattgagta
      901 gtgcatcaaa cgtttcatca ttttcctccc aaaataaaag tgaataaact actgccccgc
      961 gctaacgcag gggatcaatg atcgaattag aggtaccagt tggttctggc ttcgctgact
     1021 ataatcatag caaaccttac aattttgtca agtggtttta aaaaactgta aactgaagag
     1081 aaaataccct tcagcctgct atagcgataa acatggcgta tttcttcaga acaaccccaa
     1141 aacaagcgct ggagatggct tgacagactt tgccagggcg a
//
LOCUS       DAOTLW010000175         1178 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_98953_length_1178_cov_1.190561, whole genome shotgun sequence.
ACCESSION   DAOTLW010000175 DAOTLW010000000
VERSION     DAOTLW010000175.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1178)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1178
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_98953_length_1178_cov_1.190561"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..573
                     /locus_tag="P5516_09615"
     CDS             <1..573
                     /locus_tag="P5516_09615"
                     /inference="COORDINATES: protein motif:HMM:NF018884.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HRT92724.1"
                     /translation="SARDPQPDFRLWFFDGESDAQAIENQGLPLEAGDTLLLCSDGLT
                     DLVSDHEIQRLVKSTPLERTPAALIDLANARGGHDNTTVVLLRVPGERDQPVNRARKG
                     LILRGCVVGLVLISLLALAVYFGLRWQTARFDLQASPTSVVTRVLFTQEPGTPPAAET
                     APAVIEASPSPVREQGPTLTPWPTHTLQSE"
     gene            747..833
                     /locus_tag="P5516_09620"
     tRNA            747..833
                     /locus_tag="P5516_09620"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:781..783,aa:Ser,seq:tga)
     gene            871..960
                     /locus_tag="P5516_09625"
     tRNA            871..960
                     /locus_tag="P5516_09625"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:905..907,aa:Ser,seq:gct)
BASE COUNT          235 a          300 c          346 g          297 t
ORIGIN      
        1 tccgccaggg acccgcagcc tgatttccgc ttgtggtttt ttgacggtga aagcgacgcg
       61 caggcgatcg agaaccaggg cttgccgctc gaagcgggag atactctgct gttatgcagt
      121 gacggtctga cggatctcgt ctctgatcat gagattcagc gcctggtcaa gtcgactccg
      181 ttggaacgca cacctgctgc gctgattgac ctggcaaatg cgcgcggcgg gcatgataac
      241 acgactgttg tcctgctcag ggttcctgga gagcgggatc aaccggtgaa tcgcgctcgc
      301 aagggtctaa tcctgcgagg ctgtgtggtc gggctggttc tgataagttt gctggccctg
      361 gcggtttatt ttggtctgcg ctggcaaacc gcgcgttttg atttgcaggc gtcgccaacg
      421 tcggttgtaa cccgtgtact tttcacccag gagccaggga cgcctcctgc tgctgaaacg
      481 gcaccggctg tgattgaagc cagcccttcg ccagtgcgag agcaaggtcc gaccctgacg
      541 ccctggccga ctcatactct acaaagcgag taacctgcgc ctgcctttga atacgtttct
      601 gttgatgcag cttgtgagaa tgttgtgaga gccgatgaga ttttaactgc aacacagtcc
      661 ggtaagtgat tgcatcttca gtcgtgaaag gtatgattaa gtttcggctg gtagaaatcg
      721 gctattagtg ttagaatttg taacctggag ggatggccga gtggtttaag gcgcctgtct
      781 tgaaaacagg tgtgggttta cgcccaccgt gggttcgaat ccctctccct ccgcctggaa
      841 gtttgagggt aaaataaaca attgaagctt ggggaggtgc cagagtggtt gattggggcc
      901 gcctgctaag tggttgttgg gtttgtagct caaccggggg ttcgaatccc cccctccccg
      961 ctgaaggatc tggcttgcaa tggaaatttg caagcctgtt ttttaacggc tggttatcta
     1021 atcccccggg ggtctcaccg caaccaaaga ccccaagggt ttgcgttgga tcttcgccta
     1081 tctggcattc aaaccccggg ggtctaactg aactaaagac ccccggggtt tacgttgaat
     1141 cttcacctac ctggcattca aaccctgggg gtctaacc
//
LOCUS       DAOTLW010000176         1170 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_99862_length_1170_cov_7.543498, whole genome shotgun sequence.
ACCESSION   DAOTLW010000176 DAOTLW010000000
VERSION     DAOTLW010000176.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1170)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1170
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_99862_length_1170_cov_7.543498"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(128..850)
                     /locus_tag="P5516_09630"
     CDS             complement(128..850)
                     /locus_tag="P5516_09630"
                     /inference="COORDINATES: protein motif:HMM:NF014625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HRT92725.1"
                     /translation="MENPEGWKTKKKILAIFAHPDDAEFFCGGMIARWVDQGHCVSYC
                     LLTKGQRGFHEKGMDLTTMAEIRVREQMAAGSVLGVTDIRFLEHLDGELVADIALREE
                     IIQEIRKRKPDIVVSCDPTNLFPAVNRINHPDHRAAGQAVIDAVFPAAGSPAYQFNDK
                     LTATEAHQVEEMWLALTHQPNFEIVLTDYLEKRIRAILCHHSQVQATAEELLDKYLKP
                     SLAKEGADAPAYCEKFLRIIFN"
     gene            complement(863..>1170)
                     /locus_tag="P5516_09635"
     CDS             complement(863..>1170)
                     /locus_tag="P5516_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92726.1"
                     /translation="EITGMCGRLRCCLIYEYENYLEARKTLPKKNKRVITPNGEGKVV
                     DVYPLRGAVLVDIPEIGRKEFNQTEIQAADEQEVLNQAAASFVAKHSGSGCEQCKRK"
BASE COUNT          267 a          304 c          262 g          337 t
ORIGIN      
        1 caggaactct tttattcata tgattattcc gatgtttaga ttatatcaca ggcattaatc
       61 ctttacaaca gcgttctaag ctacgaattc tgtcgtttcc acgggtatta atctgatgat
      121 tgaggagtta gttaaagatg atgcgcagga atttctcgca atacgctggc gcgtccgccc
      181 cttctttcgc caaagaaggt ttcaagtact tgtcgagcaa ttcttcagcg gttgcctgta
      241 cctgagagtg gtggcacaag atagcacgaa tccttttctc aaggtaatcc gttaacacga
      301 tctcgaaatt cggctgatgg gtcaatgcca gccacatttc ttcaacctgg tgggcttcag
      361 tggctgttaa cttatcgttg aattggtacg cggggctgcc agcagctgga aaaaccgcgt
      421 ctattaccgc ctggcctgca gcccggtgat ctggatggtt aatgcggttt accgcaggaa
      481 acaagttggt tgggtcgcaa ctgacgacga tatctggctt tcgttttcgg atttcttgga
      541 tgatttcttc tctcaacgcg atatctgcga ccaattcacc atccagatgt tcaagaaaac
      601 ggatatccgt cacgcctaat acagaaccgg cagccatttg ctcccgaacc ctaatttctg
      661 ccatggtcgt caaatccatg cctttttcgt gaaaacctcg ttgacctttg gtcagcaagc
      721 aataggatac acaatgaccc tggtcaaccc atcgagcaat cattcccccg cagaaaaatt
      781 ccgcatcgtc tggatgggca aaaatggcca ggatcttttt cttggttttc cagccctcag
      841 ggttctccat tttgccagcg tctcatttac gcttgcattg ctcgcagccg ctgccgctat
      901 gcttggcgac gaaagaagcc gctgcctggt tgagcacctc ctgctcatcg gcagcctgaa
      961 tttcggtttg attaaactct tttcttccaa tttcaggaat atcaaccaga acagcgcctc
     1021 gtagggggta gacatcaacc actttgccct ctccgttggg cgtgatcacc cttttgtttt
     1081 tctttggcag tgttttgcgc gcttccaggt agttctcata ctcgtagatc aggcagcagc
     1141 gcaatcgacc acacataccc gtaatttctg
//
LOCUS       DAOTLW010000177         1162 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_100949_length_1162_cov_12.438121, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000177 DAOTLW010000000
VERSION     DAOTLW010000177.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1162)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1162
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_100949_length_1162_cov_12.438121"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            167..676
                     /locus_tag="P5516_09640"
     CDS             167..676
                     /locus_tag="P5516_09640"
                     /inference="COORDINATES: protein motif:HMM:NF027484.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HRT92727.1"
                     /translation="MTSIAETVAVDFTRTAIARPTDTPTPLPTPTNTATPVTPTIAVT
                     NTPAGTQAPTSVPGGQDAGVWVSSNPADGSNITAGQPFDVNVTLMNTGTTTWTTAYAI
                     KFINGERMGAPETIYMPIDVPPTKSITLTVRFTAPSTGGTVRSNWQIVNAAGTGFYAF
                     YFEYNVVTP"
     gene            700..>1162
                     /locus_tag="P5516_09645"
     CDS             700..>1162
                     /locus_tag="P5516_09645"
                     /inference="COORDINATES: protein motif:HMM:NF019931.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1730 domain-containing protein"
                     /protein_id="HRT92728.1"
                     /translation="MSLKDAIKAEADQLGFVLFGVAPAVSPPHFPAYQAWISAGKQAG
                     MAYLTRPEALTARSTPEQLLPGCRSILCVGLPYPPPLPPSAAEAEKDYGKIAAYATLP
                     DYHRILGDKLKALATRLPSLAGRVVRWRICVDTAPILEKDYAFLAGLGWIGR"
BASE COUNT          258 a          357 c          275 g          272 t
ORIGIN      
        1 tctacaataa gaaatcgagc gctatgtttg atgctatttt gtgcgccttg gagttgagat
       61 gaaaaataaa aagataatgg gtcgcctcct attacttgga ttgagtatag ctttattgct
      121 ttcggcatgc agttcttcca cgcccacaat gagccccgaa gaaatcatga ccagcattgc
      181 agaaactgta gcggtggatt tcacccgcac agccattgca cgccccacgg atacccccac
      241 ccccctgccc acaccgacca acactgccac accggttaca ccaactatcg cagtaaccaa
      301 tacccccgca ggcacacaag cgcctacttc cgttccaggc gggcaggatg ctggcgtctg
      361 ggtttccagc aatcccgccg atggcagcaa catcaccgca ggtcagcctt ttgacgtgaa
      421 cgtgaccctc atgaacactg gcactaccac gtggactacc gcctatgcga ttaaattcat
      481 caacggcgaa cgcatgggtg ccccagaaac catttatatg cccatcgacg tgccacctac
      541 caagtctatt acactgaccg tgcgcttcac tgcgccttct actggcggta cagtgcgcag
      601 caattggcag attgtcaatg ctgccggcac cggtttttac gcgttttact tcgaatacaa
      661 cgtcgttacc ccttgatttc tcctgctaag gagaggatta tgtccttaaa agacgccatc
      721 aaagccgaag ctgaccagct cggctttgtt ctttttgggg ttgcccccgc agtttcccca
      781 ccgcatttcc ccgcatatca ggcttggatt agtgctggca agcaggcggg catggcatat
      841 ctcacccgcc cagaggcgct cacagcccgc tccacgcccg agcagctgct gccaggctgc
      901 cgttcgatcc tctgcgtggg cctgccttat cctccccctc tcccaccttc agcagctgag
      961 gcggagaagg attatggcaa aattgctgcc tacgcaacct tacccgatta ccatcgcata
     1021 ttaggcgata agctcaaagc cttggctacg cgcttaccaa gcttagctgg gcgggttgtg
     1081 cgttggcgca tttgcgtgga taccgcccct atcttagaga aggattacgc tttcttggct
     1141 ggcttgggtt ggatcggacg ca
//
LOCUS       DAOTLW010000178         1159 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_101394_length_1159_cov_2.862319, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000178 DAOTLW010000000
VERSION     DAOTLW010000178.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1159)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1159
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_101394_length_1159_cov_2.862319"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(295..492)
                     /locus_tag="P5516_09650"
     CDS             complement(295..492)
                     /locus_tag="P5516_09650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92729.1"
                     /translation="MTPDWTQAPKGAQYFNVNTGLWYRRKFFEVQVWRDDAWKYTHAT
                     PLIPPHFIERPDDDDPSGGEK"
     gene            complement(489..911)
                     /locus_tag="P5516_09655"
     CDS             complement(489..911)
                     /locus_tag="P5516_09655"
                     /inference="COORDINATES: protein motif:HMM:NF016343.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF551 domain-containing protein"
                     /protein_id="HRT92730.1"
                     /translation="MNIEKLIHDLRARINPQYYDQLGTESYERHQVVKALEYLQQENE
                     RQRFDILAMNQSLDLLREENERLRKDAGRIEWQPIETAPKDGTDILVMTGETMHVVRW
                     INIHGDFDYWAVDDNKHGPFTLRGKAPTHWMPLPEPPK"
     gene            complement(908..>1159)
                     /locus_tag="P5516_09660"
     CDS             complement(908..>1159)
                     /locus_tag="P5516_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92731.1"
                     /translation="IAAANPAAILELIRQRDELLAALKYARELVDDWGAYATAYMQEK
                     HDLAGDLERLDAAISKVVEADEYRDSFDAAADRLLEGGE"
BASE COUNT          236 a          343 c          274 g          306 t
ORIGIN      
        1 taaagcctga gttgctcctg taagcaccgt tacaactgca tctttgatgg tcatatcaac
       61 ctcacctccc acggcgactg ttgtattgtt gatgcgtgtc cgtttttcac aagccaatcc
      121 atccacgcat atgattcgag aaaaccgata ccaaacatgc gcattatttt ggctgatcca
      181 aactggtgaa acccttccgg cccgcgcatt ttctccagca tttcaaggtt tgtcattttg
      241 cttttctcca gcgcataacg cgtgaaaacc acggatattc tcggcagaat atgctcactt
      301 ctcacctccg gacggatcat catcatccgg cctttcaata aaatgcggcg ggattagcgg
      361 ggttgcgtgc gtatatttcc atgcatcatc acgccatacc tgaacttcaa aaaacttcct
      421 gcgataccac agtccggtgt tgacgttgaa gtattgcgcg ccttttggcg cttgagtcca
      481 atctggtgtc atttcggcgg ctccggcagt ggcatccagt gggttggtgc tttcccgcgc
      541 agcgtaaacg ggccgtgctt gttgtcgtca acagcccagt aatcaaaatc accgtggatg
      601 tttatccacc tgacaacgtg catggtttcc ccggtcatga cgaggatgtc agttccatct
      661 tttggcgctg tctcaatcgg ttgccattcg atccgccccg catccttccg cagccgctcg
      721 ttttcttcgc gcagcaaatc cagcgactga ttcatggcca gaatgtcgaa acgctggcgc
      781 tcgttttcct gctgaagata ttccagcgcc tttaccacct gatgccgctc ataactctcc
      841 gtccccaact ggtcgtaata ctgcgggtta atccgggcgc gcaggtcgtg gatcagtttc
      901 tcgatgttca ttccccaccc tccagcagcc ggtcagcggc tgcgtcaaaa gaatcgcgat
      961 attcatcagc ttccaccacc tttgatatgg cggcgtccag tcgctcaagg tcgccagcaa
     1021 ggtcgtgctt ttcctgcatg tacgccgtag cgtatgcgcc ccaatcatcg accagttcac
     1081 gcgcatattt cagcgccgcc agtagttcat cccgctgccg gatcagttca aggatggcgg
     1141 cagggttggc ggcggcgat
//
LOCUS       DAOTLW010000179         1141 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_103829_length_1141_cov_20.019337, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000179 DAOTLW010000000
VERSION     DAOTLW010000179.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1141)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1141
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_103829_length_1141_cov_20.019337"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..395
                     /locus_tag="P5516_09665"
     CDS             <1..395
                     /locus_tag="P5516_09665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92732.1"
                     /translation="GMTDLSVMMNWFAPDEETRETYCARCGTHVTADRHATKILCEPC
                     RRESSILNSRNQHAKHAGGYASTGDPEIDLVMALILHAAREAKAGDRDAAQFLVAHDG
                     AELWLRFAGIGVTNQMRRKLELLAIGAE"
     gene            392..583
                     /locus_tag="P5516_09670"
     CDS             392..583
                     /locus_tag="P5516_09670"
                     /inference="COORDINATES: protein motif:HMM:NF024776.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HRT92733.1"
                     /translation="MNRPTEDEAEYNIIIAALTPTQARAVTLIRLGYRKSDIARKEGK
                     SRSTITRIFNELRNKRLFS"
     gene            804..1085
                     /locus_tag="P5516_09675"
     CDS             804..1085
                     /locus_tag="P5516_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92734.1"
                     /translation="MDFSSAVVAGIPLVLVVIGLVEWVKRLGLAGVALNIVALAIGIG
                     LGVAYQCSIVVPVDFAGWFTAGVYGVALGLVASGIYDAGKSIISGGGVG"
BASE COUNT          266 a          266 c          351 g          258 t
ORIGIN      
        1 gagggatgac tgacctctcc gtgatgatga actggtttgc gccggacgag gaaacacgcg
       61 agacttactg cgctcgctgt ggcacgcacg tcaccgctga caggcacgcg acaaagatat
      121 tatgcgaacc gtgccggcgg gagtccagca ttttgaacag ccgcaatcag cacgccaagc
      181 acgcgggcgg gtacgcctcc accggtgacc ccgaaattga cctggtgatg gcgttgatac
      241 tgcacgcggc acgtgaggcg aaggctggtg accgtgacgc tgcgcagttc ctcgtggcgc
      301 acgatggcgc ggagctgtgg ttgcggttcg ctggcattgg cgtgacgaac cagatgcgcc
      361 ggaagctgga attattagcg attggagcgg aatgaataga ccaacagaag atgaagcgga
      421 gtacaacatc attatcgccg ccctcacacc cacccaggcg agggcggtga cccttatacg
      481 tctcggatac cggaaatctg acattgcgcg caaagaggga aagagcaggt caacaattac
      541 acgaatattc aacgaattac gcaacaaacg gctgttttcg tgaccattag ttagtgagga
      601 atattatgcg aaaaacttgc gtttgcggta aatgcatcac gcggggagac ctgtgtaata
      661 tttgcctcaa tatctatggc gcaaatcggg cggagtggcc ggcatggcta aaattctacg
      721 tcaatgacat gagacgtgaa ctccgacaag agcgccgtat tgacgaacac gaaataactt
      781 ttacggactt aggagtttac taaatggact tttcaagtgc tgttgtggct ggaataccgc
      841 ttgttttggt cgtgatcggt ttggttgaat gggtgaagcg tcttggcttg gcgggcgttg
      901 cgctgaacat cgtcgcgctt gctattggca ttggtttggg cgtagcgtat caatgcagca
      961 tcgttgtacc cgttgacttc gcagggtggt tcacggctgg cgtttacggc gttgcgcttg
     1021 ggctggtcgc gtctggtatc tatgacgctg gcaagtcgat catatcaggc ggaggcgttg
     1081 ggtagttgac ggagtataaa aaccgcatcg ttggctcagg cgaggaatcg cttgaccaga
     1141 t
//
LOCUS       DAOTLW010000180         1135 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_104723_length_1135_cov_9.276852, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000180 DAOTLW010000000
VERSION     DAOTLW010000180.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1135)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1135
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104723_length_1135_cov_9.276852"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(240..>1135)
                     /locus_tag="P5516_09680"
     CDS             complement(240..>1135)
                     /locus_tag="P5516_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HRT92735.1"
                     /translation="YPFDPQLYRYWMPVDTYTGGSEHATMHLMYVRFFHKAARDCGIT
                     TGREPMRQLRNQGMVLGEDGEKMSKSRGNVIAPDDLVQRYGADTVRSYLMFFARWEVG
                     GPWNSSGIEGNARWLKRVWATLLEPAKGGASREVLRSLRRKTHQTLRMVTYDFENFEF
                     NTIISSLMELLNEMGRAKNAGAFGTPEWDEAADIYLRMLAPVCPHIAEELWERLGKPY
                     SIHTQAWPEVDEEAARDEEITLVVQVNGKLRDRIQVPVGISDAEAEQKALSSPAVQQA
                     LAGKQVRKVIVVPGKLVNFVV"
BASE COUNT          233 a          326 c          301 g          275 t
ORIGIN      
        1 gggagaacgt cttgggccgc tcgctggtta tctacggcgc cttcctgatc tggtcaggcg
       61 tttcagccct catccctacc cttgcctatg gctatgagcc tgtgtggttg gctgccattg
      121 aaattgctct gggaattgtt gggataattt ttggggtaaa gcctcaaccg gcagcataaa
      181 acacacagcg gatcacgcga tccgctgtgt taatagcttt atgattcttt aggttttcgt
      241 tagacgacga aattaactaa tttcccaggc accacaatca ctttgcgtac ttgcttgcca
      301 gccagagcct gctgaacagc tgggctggag agagcttttt gctcggcttc ggcatcgcta
      361 atccctaccg gcacttggat gcggtcacgc agcttgccgt tcacttgcac cactaacgtg
      421 atttcttcat cccgtgcagc ttcctcatcc acctcgggcc aagcttgggt gtggatggaa
      481 taaggttttc ccaggcgctc ccacagctcc tcggcgatat gcgggcagac gggggctaac
      541 attctcaagt aaatatcagc agcctcatcc cactcgggcg tgccgaaggc tccagcgttt
      601 ttggcgcgcc ccatctcgtt gagcagctcc atcaggctgg agatgatggt attgaactcg
      661 aagttttcga agtcataggt aaccatccgc agggtttgat gcgttttgcg ccttagagaa
      721 cgcagcacct cacgagaagc gccgccttta gccggctcga gcaacgtcgc ccaaacgcgc
      781 ttgagccagc gggcattgcc ttcgatgccg ctgctattcc atggaccgcc cacttcccaa
      841 cgggcaaaaa acatcaggta ggaacgcacg gtatcggcac catagcgctg caccaggtca
      901 tccggtgcaa tcacattgcc gcggcttttg gacatcttct cgccatcttc gcccagtacc
      961 atgccttggt tgcgcagttg gcgcataggc tctcgcccgg tggtgatacc gcagtcccga
     1021 gcggctttgt ggaagaagcg cacatacatc aggtgcatgg tagcgtgctc gctgccgccc
     1081 gtataggtat ctacaggcat ccaatagcga tacagctgcg ggtcaaaagg atatt
//
LOCUS       DAOTLW010000181         1134 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_104850_length_1134_cov_11.578313, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000181 DAOTLW010000000
VERSION     DAOTLW010000181.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_104850_length_1134_cov_11.578313"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1134
                     /gene="dnaE"
                     /locus_tag="P5516_09685"
     CDS             <1..>1134
                     /gene="dnaE"
                     /locus_tag="P5516_09685"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HRT92736.1"
                     /translation="LRFNLLFERFLNPGRVTMPDIDLDFQDDRRAEMMEYCNQKYGAD
                     KVAQIITYSTIAARGAIRDVGRVMNIPLPEVDRVAKMIPGLQQGKSPTIAETLEKSPE
                     LRSVYDSSPQMKKLIDTASRMEGSIRNVGTHAAGVIISDQPITEYLPLHRPTSQSEDL
                     PIKSVSQYDMDGVNYLGLLKVDFLGLATLTIMARACELIEQRHGVHLTLENIPIDDPE
                     VFRYISEGHTMGTFQLEGSGMTRYIMQMQPTDISHVIAMIALFRPGPMESIPEYIERM
                     HGKKEVRYPHEKLVPILKDTYGHAVYQEQIMQAAVELAGYTPSESDDLRSAISKKKEK
                     EIAKHHKKFVEGAVKQGIPREEAEAIFNDWESFAHYGFNKSHAA"
BASE COUNT          300 a          313 c          275 g          246 t
ORIGIN      
        1 ctgcgcttta acctcctctt cgagcgcttc ttgaaccccg ggcgtgttac catgccggat
       61 attgacctgg acttccaaga tgaccgccgc gcggagatga tggaatattg caatcagaaa
      121 tatggcgcgg ataaagtcgc ccaaatcatc acctacagta ccatcgcagc gcgtggagcc
      181 atccgcgatg ttggtcgcgt gatgaacatt ccactcccgg aagtggaccg cgttgcaaaa
      241 atgatccccg gcttgcagca aggcaaaagc ccaaccatcg ccgagacctt agagaaatct
      301 cccgagctgc gctctgttta cgattcatct ccccaaatga aaaaactgat tgatactgcc
      361 agccgtatgg aaggttccat ccgcaatgtg ggtacccatg ctgccggcgt gattatcagc
      421 gaccagccaa ttaccgagta cctgccgctg caccgcccaa ccagccagag cgaagactta
      481 cccatcaagt ccgtatccca atacgatatg gacggtgtca attaccttgg tttgctcaaa
      541 gttgacttct tgggtttggc tacgctcacg attatggcga gagcttgtga gctgatcgag
      601 cagcgccatg gcgtccacct gaccttggag aacatcccca ttgatgatcc tgaagttttt
      661 cgctacatta gcgaggggca taccatgggc accttccaat tggaaggctc ggggatgacg
      721 cgctacatca tgcaaatgca acccacagat atttcacatg tgattgcaat gatcgcgctt
      781 ttccgccccg gtcctatgga aagcatccca gaatacatcg agcgcatgca cggcaagaaa
      841 gaggtcaggt atccccacga gaagctggtc cctatcctca aagatactta cggacacgcg
      901 gtctaccaag aacaaattat gcaagccgcg gtagagttgg ctggctatac cccctccgaa
      961 tctgacgact tgcgctcggc gatctccaag aaaaaagaga aggagattgc caaacaccac
     1021 aagaaatttg ttgaaggcgc agttaaacag ggtattccac gtgaagaagc tgaagccata
     1081 tttaacgatt gggaaagctt tgcccattat ggcttcaata agagccacgc tgcc
//
LOCUS       DAOTLW010000182         1129 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_105617_length_1129_cov_13.321229, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000182 DAOTLW010000000
VERSION     DAOTLW010000182.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1129)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1129
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105617_length_1129_cov_13.321229"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1129)
                     /locus_tag="P5516_09690"
     CDS             complement(<1..>1129)
                     /locus_tag="P5516_09690"
                     /inference="COORDINATES: protein motif:HMM:NF012356.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HRT92737.1"
                     /translation="NWRNPAVKEAMFNVARFWLDMGVDGFRLDAIGTIFEDENYPPIP
                     DGMSQEELYRLARNTQNPEADEKVLSYWARMYRYQSDQPEVHHLMKELRILLDEYEDR
                     VLIGETDDLAFYGDGNDELHLNFNFPLMRTQRLTAKHVRKNQQERLAALPVYAWPCNT
                     LGNHDSARVYSRYGDGENNPTIARLNLMLLLTLRGTPFLYNGEEIGMSDLIIEDPGRF
                     RDPLSLLYAALDQKVMGSDKKTATLTGAMMGRDRNRTPMQWMDAPHGGFCPADIEPWL
                     PVNSDYHTGINVNAQEKDPGSLLNFYRALIKLRKQSPTLQTGAYEEIDAGNDEVLVFR
                     RWDGSGSYIVALNFSDALQRAYLPDNPGMNIFSNIPNSAGWE"
BASE COUNT          252 a          303 c          283 g          291 t
ORIGIN      
        1 tttcccatcc cgctgaattg ggaatattac taaatatatt catgccgggg ttatcaggga
       61 gatacgcccg ttgcagcgca tcgctgaagt ttaatgccac aatataactg cctgagccat
      121 cccagcgtcg aaaaaccaga acttcatcgt ttcccgcgtc aatctcttca tatgcgccgg
      181 tttgcagcgt tggagactgc tttcgcaact taatcagggc gcggtagaaa ttcagcaatg
      241 agcccgggtc tttttcctgc gcgtttacat ttattcctgt gtggtaatcg gagttcaccg
      301 gcagccaggg ctctatatca gcggggcaaa aacccccatg aggggcgtcc atccactgca
      361 tgggcgtacg gttgcggtct ctgcccatca tggcgcctgt cagcgttgcg gtctttttat
      421 ccgagcccat cactttctga tccagcgcag cgtataaaag gctgagcggg tcgcgaaacc
      481 tgccggggtc ttcaatgatg aggtcagaca tgccaatctc ctctccattg tacaggaaag
      541 gcgttcctcg caaagtcagc agcagcatca ggttcagcct ggcaatagtg ggattatttt
      601 ctccatcacc atatcgagaa tagacgcggg cagagtcatg gtttccaaga gtattgcatg
      661 gccaggcgta aactggcaag gcagccagcc gttcttgttg gtttttcctc acatgttttg
      721 ctgtcaacct ttgggtgcgc atcaatggaa aattgaaatt gagatgcagt tcatcgtttc
      781 cgtccccgta aaaagcaagg tcgtcggttt ccccgatcag cacccggtcc tcgtattcat
      841 ccaggagtat tcgaagttcc ttcatcaggt ggtgcacttc aggttgatca gattgatagc
      901 ggtacatgcg agcccagtac gagagcactt tttcatccgc ttcagggttc tgggtgttac
      961 gggccaggcg gtacaactcc tcctggctca tcccatccgg gatgggaggg taattttcgt
     1021 cttcaaaaat ggtgccaatc gcgtccagcc tgaaaccatc cacccccatg tccagccaaa
     1081 aacgcgccac gttgaacatg gcttctttaa cagccgggtt acgccagtt
//
LOCUS       DAOTLW010000183         1127 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_105924_length_1127_cov_5.700560, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000183 DAOTLW010000000
VERSION     DAOTLW010000183.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1127)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1127
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_105924_length_1127_cov_5.700560"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="P5516_09695"
     CDS             complement(<1..923)
                     /locus_tag="P5516_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92738.1"
                     /translation="MRKTKSLAILSVIVLFLLMGTACGVIGSKGVPSEDSIATSVAAT
                     VNAQPGGDLAPAATDQPGNLPLLDTASPSDTAATVPPLYAYPVSVAFVAPDKNAYYWN
                     ESLSSPVTLTSSADVERAVVSPDGTQVALTRTTDWVNYTLEVVNTDGSGLRTLLSPSG
                     FAALPRPADSVASIPNQLSWVPGTHALAMTTRITYDGPGYQTGENLYLVDTTTSEIRA
                     LFTVSTQWGWHYAFSPDGSKIAVSFPEGMDIYNSSGSKLDHPVLTYPFINTASEYAWV
                     ASPVWSANSTTLVTVVPPQEPWATSPANSSV"
BASE COUNT          275 a          271 c          333 g          248 t
ORIGIN      
        1 tacacgctcg aattggcagg cgaggttgcc cagggctcct ggggcggaac gactgttacc
       61 agggtagtgc tgttagcaga ccaaactggc gatgccaccc aggcgtactc agaggctgtg
      121 ttaatgaatg ggtatgttag aactgggtgg tccagctttg aaccgctgga attgtagatg
      181 tccattcctt cagggaatga gaccgcgatt ttactgccat caggcgaaaa ggcgtaatgc
      241 cagccccact gagtagaaac agtgaagagc gcccgtattt cagaggtagt ggtatcaacc
      301 agataaaggt tctcaccggt ttgatagccc ggaccatcat aagtaatacg cgtggtcatg
      361 gcgagcgcat gggtgcccgg tacccagctt agctggttgg ggatggacgc gaccgaatca
      421 gcggggcggg gtagcgcggc gaaccctgaa ggagagagga gcgtgcgcag accactgcca
      481 tccgtattga ccacttccag cgtgtagttc acccaatcgg tggtgcgggt gagggctacc
      541 tgtgtaccat ctggagaaac gaccgcacgc tccacgtcag cgctgctggt gagggtgacc
      601 ggagaggata aactctcatt ccagtaatag gcgtttttat caggcgcgac aaaggcaacg
      661 ctcacagggt aagcgtataa cggtggaacc gtggcggcag tatcggatgg ggaggccgtg
      721 tctaacagcg gaagattccc aggttgatcg gtggctgcgg gagccaggtc tccgcctggt
      781 tgggcgttga ccgtggccgc cacgctggtg gcgatggaat cttctgaagg cacgcctttc
      841 gagcctatca ctccgcaggc agtacccatc aagaggaaca agacgattac ggacaggatg
      901 gctaacgatt tcgtttttcg catcacaaca cctccaattg tgatattaag atgaacttac
      961 tcttattaaa gattatatca ttttctctgt aagaacgtga caaatcccgc tcatgaaaaa
     1021 tgctaggcag gagggcgttt ttttgtcttc gctttgactc agttaagcga ttaagaaacc
     1081 aacacccatc aacagcggcg ttaccaggtt gagcagcaca ttttgaa
//
LOCUS       DAOTLW010000184         1123 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_106546_length_1123_cov_4.411049, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000184 DAOTLW010000000
VERSION     DAOTLW010000184.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1123
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_106546_length_1123_cov_4.411049"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="P5516_09700"
     CDS             complement(<1..923)
                     /locus_tag="P5516_09700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92739.1"
                     /translation="MRKTKSLAILSVIVLFLLIGTACGVIGSKGVPSEDFIATSVAAT
                     VNAQPGGDLAPAATDQPGNLPLSDTASPSDTAATVPPLSVYPVSVAFVAPDKNAYYWS
                     ESLSSPVTLTSSADVERAVVSPDGTQVALSRTTDWVNYTLEVVNADGSGLRTLLSPSS
                     FAALPRPADSVASIPNQLSWVPGTHALAMATRITYDGPGYQTGENLYLIDTTTSEIRA
                     LFTVSTQWGWHYAFSPDGSKIAVSFPEGMDIYNSSGSKLDHPVLTYPFVNTASEYAWV
                     ASPVWSANSNTLVAVVPPQEPWATSPANSSV"
BASE COUNT          281 a          266 c          329 g          247 t
ORIGIN      
        1 tacacgctcg aattggcagg cgaggttgcc cagggctcct ggggcggaac gactgccacc
       61 agggtattgc tgttagcaga ccaaactggc gatgccaccc aggcgtactc agaggctgta
      121 ttaacgaatg ggtatgttag aactgggtgg tccagctttg aaccgctaga attgtaaatg
      181 tccatccctt cagggaaaga gaccgcgatc ttactgccat caggcgaaaa ggcgtaatgc
      241 cagccccact gtgtagatac agtgaagagc gcccgtattt cagaggtagt ggtatcaatc
      301 agataaaggt tctcaccagt ttgatagcca gggccatcat aagtaatacg tgtggccata
      361 gcgagcgcat gggtgcccgg tacccagctt agctggttgg ggatggatgc gaccgaatca
      421 gcggggcggg gtagagcggc gaagcttgaa ggagagagga gcgtgcgcaa gccactgcca
      481 tccgcattga ccacttccag cgtgtagttc acccaatcgg tggtgcggga gagggctacc
      541 tgcgtgccat ctggtgaaac gaccgcacgc tccacatcgg cgctgctggt gagggtgacc
      601 ggagaggata aactctcgct ccagtaatag gcgtttttat caggcgcgac aaaggcaacg
      661 ctcacagggt aaacggataa cggtggaacc gtggcggcag tatcagatgg ggaggccgtg
      721 tctgaaagcg gaagattccc gggttgatcg gtggctgcgg gagcaaggtc tccgcctggt
      781 tgggcgttga ccgtggccgc tacgctggtg gcgatgaaat cttctgaagg cacgcctttt
      841 gagcctatca ctccgcaggc agtgccaatt aagaggaata atacgattac ggacaggatg
      901 gctaacgatt tcgtttttcg catcacgaca cctccaattg cgatattaag atgaacttac
      961 tcttattaaa tattatatca ttttctctat aagaacgtga caaatcctgc tcatgaaaaa
     1021 tgccaggtcg gaggtttttt gtcttcgctt tgacacagtt aagcgatgaa gaaaccaaca
     1081 cccatcaaca gcggcgttac caggttgagc agcacatttt gaa
//
LOCUS       DAOTLW010000185         1117 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_107459_length_1117_cov_10.974576, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000185 DAOTLW010000000
VERSION     DAOTLW010000185.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1117)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1117
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_107459_length_1117_cov_10.974576"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            420..1010
                     /locus_tag="P5516_09705"
     CDS             420..1010
                     /locus_tag="P5516_09705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HRT92740.1"
                     /translation="MPYPYHSICVFCGSADGLAPVYYTAAYEMGELLARNGITLVYGA
                     GRTGMMGALAEGALGAGGEVIGIVPNGLESPQLIYTTGLTRLEVVTDIQLRKARMNAL
                     ADAFITLPGGYGTMDEMFEVLTWSQIGRHRKPAAFLNVDGYFDSLLAWIERAYQDHFI
                     YEEHLDLYVTDASPAKLLEKLAAFTFPGNIERWLVR"
BASE COUNT          205 a          290 c          373 g          249 t
ORIGIN      
        1 ggattgggtg ccggtgcgcc agctttcgct ggtgctgggc gcgcatgtgg gtcgcagcat
       61 ggtgggggta tgcttcgcgc cggcagacac gttcgtaggg ctgccgtaga aagctggctt
      121 gaaaccggcg cgggcaggga ggtcctccct gcctgttttt tttaacctgt gcgggtcagg
      181 cgggatggcg tgggcagtct tcataactgc gcgtagtcat tatcatcccc aaagctggat
      241 agtcatcaga tgcgttgaag ccttcacact agtgaaaggg caggggaggt gctcgtacct
      301 ccatcagcgg gttcgaggac gggcgatctc tttttgatgg cgccgcgcca gttccggtat
      361 aatggagtat cccgcgtgct cccggctgga gagccgcgac gattggctta aggagaacca
      421 tgccatatcc ctatcacagc atttgcgtgt tctgcggttc agcagacggg ctggcgccgg
      481 tgtattacac cgccgcttat gaaatgggag agctgctggc gcggaacggt atcacgctgg
      541 tgtacggggc ggggcgcacc ggcatgatgg gcgcgctggc agaaggcgcg ctgggagcgg
      601 gcggagaagt gataggcatt gtgcccaatg ggctggaaag cccgcagctc atttacacta
      661 ctgggctgac ccgcctggaa gtggtgacgg acatccagct gcgcaaggcg cgcatgaacg
      721 cgctagcgga cgcttttatc accctgccag gagggtacgg caccatggac gagatgttcg
      781 aggtgctcac ctggtcgcag atcgggcggc accgcaaacc cgcggcgttc ctcaatgttg
      841 acggttactt tgattcgctg ctggcctgga tcgagcgggc gtaccaggac cattttatct
      901 atgaagagca tcttgacctt tacgtcactg acgcgtctcc ggcgaagctg cttgaaaaac
      961 tggcggcttt taccttcccc gggaatattg agcgctggtt ggtgcgctag aagcgagtga
     1021 gagaagatga tgaaacccac acgcctgttc ctgatcatcg gtattctctt tggggtggga
     1081 ttggccgttt tcgccccttt cggcactgga tttgacg
//
LOCUS       DAOTLW010000186         1099 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_110230_length_1099_cov_8.435824, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000186 DAOTLW010000000
VERSION     DAOTLW010000186.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1099)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1099
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110230_length_1099_cov_8.435824"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            392..826
                     /locus_tag="P5516_09710"
     CDS             392..826
                     /locus_tag="P5516_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002530384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HRT92741.1"
                     /translation="MRPDWDSYFMKIAFAVSERSTCDRAFVGSVLVLDKRILTTGFNG
                     SPMGQPHCDEIGHLLVDGHCVRTIHAETNAIIQAALHGVSTRGCTCYVTHFPCINCTK
                     ALINAGVTRLVYDIAYRIDENALEFLRAADIEVVQHHFDPHH"
BASE COUNT          253 a          326 c          265 g          255 t
ORIGIN      
        1 ctcatcatac taaatttaca cgcagagcag cgagagcgca caaccctctc gctgctcttc
       61 agttaaagat gccgcccaca agatatgctt gaatgatacc ctgtagtctc cattcacaag
      121 accggtggga ggttatccga ttgggctttc ccggttagcg tatcgtcggc gtatgaaaga
      181 tcgcccctga acttttcagc agaaggcaac gaccggcggg tacgcgaagc gccggcaagc
      241 gctgaacgac tgaaagctcg cgtagggctg ctaccgacgg acccgtgtac ggtcctcgcg
      301 tcagcaacca atgctctcac gtgtgctgtc gtacaatttt taatccaagt gattcttctt
      361 atgctatcat taggcgcaag gagactgaac aatgcgacct gattgggatt cctacttcat
      421 gaagatcgcc ttcgctgtat ctgaacgcag cacctgtgac cgggcttttg tcggcagcgt
      481 gttggtgctg gacaagcgca ttctcaccac ggggttcaat ggctcgccca tggggcaacc
      541 gcactgcgat gaaatcggtc atctgcttgt ggatgggcat tgcgtgcgca ccatccacgc
      601 ggaaaccaac gccatcattc aagcagcgct gcatggggtt tcaacccgcg gctgcacctg
      661 ctacgtgacc catttcccgt gcatcaactg cacgaaagcg ctcatcaacg ccggcgtcac
      721 ccgcctcgtt tatgatatcg cctaccgcat tgatgaaaac gcgttggaat ttctccgcgc
      781 cgctgatatc gaagtcgtgc agcatcattt tgacccgcac cactgaacca ggtggctttc
      841 tctgccccta cccgctatct ttcattgtca cccttgcggg atgaatcccg tttttttcca
      901 ggagggcgaa gccccaacca cgcgcctgcc cccgctaaac gcatgctgat attggagaaa
      961 aacgccaaga gaaattcaac ccgccagccg ccgatctctg gttcccaacc tcttctcaat
     1021 ttttttcgac tacatcgtga ccatagccaa gatatcgccg tataataagc attaccgtga
     1081 gtatcaaggt aacagaagg
//
LOCUS       DAOTLW010000187         1097 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_110567_length_1097_cov_11.179463, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000187 DAOTLW010000000
VERSION     DAOTLW010000187.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1097)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1097
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_110567_length_1097_cov_11.179463"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="P5516_09715"
     CDS             complement(<1..1076)
                     /locus_tag="P5516_09715"
                     /inference="COORDINATES: protein motif:HMM:NF013700.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HRT92742.1"
                     /translation="MSTYPNTPPESDDLYLSEVQPASKAGGSFLAKLWQRLVSLGLDE
                     RIARLGSVFFTALLLVGVVLIMGRFYANPSGTAANAELRLAAVAEGTNASVAIPAFVV
                     AESDTAIDRQISLHTILPALPRTEIITYTIQPGDSLFSIAEKFGLKPETILWGNRYIL
                     GDDPHMIYAGQELLILPVDGTLHRWSAGEGLNGVAEYYGVTPEDIINYPANHLSAATI
                     GDYTNPNIAPGTLLVVPGGKGEFPDWRTPRITRENPATAVNVGPGACTGSYDGVMGTL
                     IFTWPVANHYLTGYDYDPEANHFGIDIFGELGEPVHAADNGVVVYAGWNDWGYGEMVV
                     IDHGQGWQSLYAHLSTVEVSCGQEV"
BASE COUNT          263 a          312 c          295 g          227 t
ORIGIN      
        1 acctcctgac cgcagcttac ctccacggtg ctgaggtgcg catagagcga ctgccagccc
       61 tgcccgtggt cgatgacgac catctcgcca taaccccagt cattccagcc ggcatagacc
      121 accacgccgt tatctgcggc atgtacgggt tcacccaact cgccaaatat atcgatacca
      181 aaatgattcg cctcggggtc gtagtcataa ccggtaaggt agtgatttgc gacgggccaa
      241 gtgaagatga gcgtgcccat cacgccatcg taagagccag tacaggcgcc aggacctacg
      301 ttcaccgcag tggctggatt ctcgcgcgtg atgcgcgggg tgcgccaatc ggggaactcg
      361 cctttgccgc cagggaccac cagcagcgtg ccgggagcaa tattggggtt ggtgtaatcg
      421 ccaatcgtgg ctgcgctaag atgattagcg ggatagttga taatatcttc tggcgttacg
      481 ccataatatt ccgccacgcc gttcaagcct tcaccagcgc tccagcgatg cagcgtccca
      541 tccacgggga ggatgagcag ttcctggcct gcatagatca tatgtgggtc atcccctaaa
      601 atatagcggt taccccataa gatcgtttcg ggcttgagcc caaatttttc tgcgatagaa
      661 aacagcgaat cgccgggctg gatggtgtaa gtgataatct cagtgcgcgg cagcgccggc
      721 aaaatggtat gcaacgaaat ctgacggtca atcgcagtgt cgctctccgc caccacaaaa
      781 gcagggatag ccaccgatgc attggttccc tcggcaacag cggctaagcg taactctgcg
      841 ttggcagcgg ttccgctggg attggcatag aagcgcccca taatcaacac cacacctacc
      901 aaaagcagcg cagtgaaaaa caccgagcct aagcgggcaa tgcgttcatc taagcccaaa
      961 ctgaccaagc gctgccaaag cttagcaagg aaactgccgc ctgctttgga tgccggttgc
     1021 acctcgctga gatataaatc atcgctttcg ggtggtgtat ttggataagt tgacatgctg
     1081 ccgctatgat atcacga
//
LOCUS       DAOTLW010000188         1094 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_111036_length_1094_cov_4.170356, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000188 DAOTLW010000000
VERSION     DAOTLW010000188.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1094)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1094
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111036_length_1094_cov_4.170356"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1094
                     /locus_tag="P5516_09720"
     CDS             <1..>1094
                     /locus_tag="P5516_09720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92743.1"
                     /translation="RKKIGFAFGIFLALLAILQGAYGIALYREIGSVNVKLENPEMLA
                     GVLLLLFAIAVFKWLTSPTDTRWAILSGGLLGLSVLIRVNPIFIIPFLILAVFLAGKG
                     MFKKVFTTVLFAGLAFLLVFSPWFFSATNNQGKNHYISKIEEVINSRFFMRHGDNKAE
                     TDSEVSSPSTAFPPHVQAETGSNEPHEILHYKIGEIDQAGVPGILFHFLNNVYTGLAK
                     LPTSLVLHSIQEQVKDGIWAFTEAQPIWKTRLRLENLIAMGINLILVLIGIMAAWKRF
                     GWAGLSGVIIQTGYYSGNAVSQTSGGRYLEPVVWVLLLYYCLGLFTLSSCGIKVFSGI
                     AAEGSLKPNAAGQTSAGLPGRRRSLAIPSL"
BASE COUNT          236 a          267 c          298 g          293 t
ORIGIN      
        1 ggcgtaagaa gattggtttt gccttcggga tattcctcgc gctgctggct attttacaag
       61 gggcttacgg cattgccctc taccgcgaga ttggcagcgt gaatgtcaag ctcgagaacc
      121 ctgaaatgct ggcaggagtt ttgctactgc tgttcgccat tgcggttttt aaatggttga
      181 caagccccac agatacacgt tgggcgatcc tgagcggggg gctgctggga ctgagcgttt
      241 taattcgcgt caacccgatc tttattatcc cgttcttgat cctggcagta ttcctggcgg
      301 gcaaaggcat gttcaagaag gtgttcacca cggttttatt tgccgggttg gcttttctcc
      361 tggtgtttag cccttggttc ttttctgcga ccaacaacca ggggaaaaac cattacattt
      421 ccaagattga agaggtcatt aacagccgtt tcttcatgcg ccacggtgac aataaagctg
      481 aaacggatag cgaggtctcg tctccctcta cggcgttccc tccacatgtg caggcagaga
      541 caggctccaa tgaaccgcat gagatactgc attataagat tggggagatc gatcaggctg
      601 gggttccagg catccttttt catttcctaa ataatgttta taccggcctg gctaaattgc
      661 ccaccagcct ggtcttgcat tccatacagg aacaggtcaa agatggcata tgggctttta
      721 ccgaagcgca gccaatctgg aagacccgcc ttcggttgga gaatctgatt gctatgggca
      781 tcaacctgat cctggtgctc attggcatta tggccgcatg gaaacgcttt ggctgggcag
      841 ggctcagcgg ggtgatcatt cagacggggt actacagcgg gaacgcggta tcacaaacct
      901 caggcggtcg ttaccttgag ccggtggtct gggttttgct tttgtattat tgccttgggt
      961 tattcacatt aagctcatgt ggaatcaagg tcttctcagg aattgccgct gaaggttcac
     1021 taaaaccaaa cgccgcgggg cagaccagcg ctggtttacc gggcagacgg cgcagtttgg
     1081 ctatcccctc gctg
//
LOCUS       DAOTLW010000189         1092 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_111319_length_1092_cov_8.904532, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000189 DAOTLW010000000
VERSION     DAOTLW010000189.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1092)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1092
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111319_length_1092_cov_8.904532"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..803
                     /locus_tag="P5516_09725"
     CDS             <1..803
                     /locus_tag="P5516_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92744.1"
                     /translation="PNQVIPITLVSPREVPQSTPSGTETPTGGETTPVPLFVVGETVK
                     IQAGVIHDHNQHIVPDGTVVQFTIRIAGDQAIIAQPQATTVDGIATIEYRIERDGIFE
                     VTASSEPANTSGILILNTQGGLAQLIMPTPTPTNTPTPTPTLTPTTEVSPITTETPPP
                     NPSGYPDMIDWLMTLIFMALGFGAAYATGRFWWGSQQWGIRSGLCALIGGFSTYLLLT
                     LGIGGLEDLVREGGTWFIVEVTLVGMLIGWVAALLWWMTVSNPPSSTR"
BASE COUNT          264 a          301 c          273 g          254 t
ORIGIN      
        1 atccgaacca ggttatccca atcactttgg tgagcccaag ggaagttcca cagagcacac
       61 caagcggtac cgaaacgcca acaggcggag agacaacccc tgttccgctc tttgtcgttg
      121 gagaaacagt aaaaattcag gcgggcgtga tccatgacca caatcagcat attgtgcctg
      181 atggcactgt ggtgcaattt actattcgca tagctggcga tcaggcgatc attgcccaac
      241 cccaagccac taccgtggat gggatagcca ccattgaata tcgtatcgag cgggacggca
      301 tctttgaagt aaccgccagc agtgaacctg ccaatacatc tggcatatta atcctcaata
      361 cccaaggtgg tctggcgcag ttgatcatgc ccacacccac cccaaccaat acccctacgc
      421 ctaccccaac actgacccca accactgaag ttagccccat cacaacggaa acaccgcctc
      481 ctaaccccag cggttatccc gatatgatcg attggttgat gacgctcatt tttatggctt
      541 tgggctttgg cgcagcctac gccacggggc gtttctggtg gggtagccag cagtggggca
      601 tccgttctgg gctctgcgct ctcattggcg gttttagcac ttatttgctg ctcaccttgg
      661 gcattggagg tttggaagac ttagtgcgcg aagggggcac ttggtttatc gtggaagtca
      721 ccttggtcgg gatgcttatc ggctgggtgg cagccctgct ctggtggatg accgtgagta
      781 atccaccatc aagtacccgt taaccttcgc gaggtcgatt aagagtgcgc acactacagc
      841 gtaagttata gcgtcgcact ctactccgct ttacccaaca agattttcgg tattgaaggg
      901 cattataaaa agccctcttc agtcaaactg gtatgtaatt catccagcaa taaaatcaga
      961 gattgcgcag cagggttaac agcaaagtga ggaaacctaa actcgcggct actgcaatca
     1021 agcgctgctc taacgccccg attttggctt gccggcgcgg gctgccattg ctataaccat
     1081 tgcggaggct tg
//
LOCUS       DAOTLW010000190         1089 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_111799_length_1089_cov_8.097679, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000190 DAOTLW010000000
VERSION     DAOTLW010000190.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1089
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_111799_length_1089_cov_8.097679"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1089)
                     /locus_tag="P5516_09730"
     CDS             complement(<1..>1089)
                     /locus_tag="P5516_09730"
                     /inference="COORDINATES: protein motif:HMM:NF012677.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HRT92745.1"
                     /translation="IKPGDTILGMDLLHGGHLTHGSPVNRSGMWYNALHYTVDPQTEC
                     LDYDQIAKIAEENHPKVIIAGYSSYPWVPDWSKFKQIAESVGAYLFADVSHIAGFIAA
                     GLCPSPVGIADVISFTTHKTLCGPRGAVIITHNKDIGEKIDKAVFPGEQGGPHVNTIA
                     AMALTFELAKSDQFKALQAQTLKNTAALAEAFQKNGVRVPYNGSNTHLLLIDAKSFRG
                     VDGVPLKGDMAARILELAGIVANRNTIPGDASAFAASGVRFGTTWLTQRGFVETEMVA
                     IANLITELLRATTPYLLCAQVGKKTHAKVDFETLMNVSMRVRELADSKPAMDPTTVRH
                     GLPHYVYLDDAYEGEQAAFELSGEKVRSY"
BASE COUNT          264 a          268 c          293 g          264 t
ORIGIN      
        1 ataggagcgg actttctcac cggaaagctc aaatgctgcc tgctctccct catacgcatc
       61 atccaggtaa acatagtgcg gtaagccatg gcggacggta gtcggatcca tcgcaggctt
      121 agaatcagca agttcgcgta cgcgcatgct gacgttcata agggtttcga aatccacttt
      181 ggcatgtgtt ttctttccaa cttgcgcaca gagcagataa ggcgtggtgg cacgtaagag
      241 ttcggtgatc aggttagcaa ttgcgaccat ctcggtttcg acaaagccgc gctgcgtgag
      301 ccaggtggtg ccaaaacgca cgccgctggc agcgaaagct gaagcatcgc cgggaatggt
      361 gttgcggtta gccacgatcc ctgccaattc aaggatgcgc gcagccatat cacccttgag
      421 cggaacccca tccacgccgc ggaaagactt ggcgtctatg agcaagaggt gagtgttaga
      481 accgttatag ggcactctca ccccattttt ctggaaagct tctgccagcg cggctgtgtt
      541 tttaagcgtt tgggcttgta atgccttgaa ttgatccgat ttcgctaact caaaagtgag
      601 tgccatggca gcgatggtat tgacatgtgg accgccttgt tcgccaggaa aaacggcttt
      661 gtcgattttc tcgccgatat ccttgttatg ggtgataatc accgcaccgc gtggaccaca
      721 taaggtttta tgggtggtga agctaatcac atccgctata cccaccggag aagggcataa
      781 tccagcggca ataaaaccgg caatgtgaga aacatccgca aaaagatatg cgccgacact
      841 ttcggcaatt tgtttgaatt tgctccaatc cgggacccag gggtaggaag agtagccggc
      901 gataatcacc tttggatggt tttcctcagc gatttttgcg atctgatcgt agtctaaaca
      961 ttccgtttgg gggtcaacgg tatagtgcaa ggcgttatac cacatacctg agcggttgac
     1021 aggtgaacca tgcgttaggt gcccaccgtg caataaatcc atgcccagga tggtatcgcc
     1081 aggtttaat
//
LOCUS       DAOTLW010000191         1080 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_113400_length_1080_cov_7.046829, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000191 DAOTLW010000000
VERSION     DAOTLW010000191.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1080)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1080
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113400_length_1080_cov_7.046829"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            113..>1080
                     /gene="der"
                     /locus_tag="P5516_09735"
     CDS             113..>1080
                     /gene="der"
                     /locus_tag="P5516_09735"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HRT92746.1"
                     /translation="MSKPVVALVGRPNVGKSTLFNRLCGERLAIVDDTPGTTRDRLLA
                     EAEWSGRVFDVVDTGGIDPRAGSRGAPLSVGSADFIEEIRAQAVVAVTEADAVLFLVD
                     AISGVTPADKEIAEILRKAQKVKEGVPHPPILLVVNKADSDRQRDMAHEFYELGLGDP
                     HPISAGHGTGTGDLLDALIAVLPRQAQAVEDPSVKIAIVGKPNVGKSSLLNRLVGEER
                     SIVSDIPGTTRDAVDTAITYEGIPVTLIDTAGIRRRGKIDPGVEKYSVVRSLRAIERC
                     DVALLMIDAVEGISAQDTHIAGYVKDESKSTVVIVNKWDLLEKDNFT"
BASE COUNT          254 a          267 c          322 g          237 t
ORIGIN      
        1 gattgagagc atttctcccg ccactttcga agtgaccatt cgtacaaaaa agtaacgtat
       61 tccgcagggt ggctctggat taagggtaca ataggtctct gtatgaggac tcatgtctaa
      121 acctgttgtt gctctggttg ggcgccccaa tgttggaaaa agcactctgt ttaatcgtct
      181 ctgtggagaa cgactggcca ttgtagacga tacccccggc accacccgcg accgcctttt
      241 ggctgaagca gaatggagcg ggagggtgtt tgatgtggta gatacaggtg gtattgaccc
      301 gcgagctggc agccggggcg caccgctttc agtgggatct gccgacttta tcgaggagat
      361 tcgtgcgcag gcagtggtcg ccgtgaccga agcggacgcg gtactttttc tggtagatgc
      421 gatcagcggc gtcacacctg ctgacaaaga aatcgctgaa atactgcgta aagcgcaaaa
      481 agtgaaggaa ggagttcccc accctcccat tctgctggtg gtcaataaag ctgacagcga
      541 caggcagcgg gatatggcgc atgaatttta tgaattggga ttgggagatc cgcacccaat
      601 ctcagcggga catggcaccg ggacaggcga cctgctggac gccctcatcg ctgttttacc
      661 ccggcaagcg caagcggttg aggacccttc cgtaaagata gccattgtcg gtaaacccaa
      721 tgtgggtaag tccagcctgt tgaaccgcct ggtgggtgaa gaaaggtcga ttgtcagtga
      781 tatcccgggc accacccgcg atgcggtgga taccgccatc acttatgaag gcattccggt
      841 gacactcatt gacaccgccg gtatcagacg ccgcggcaag attgaccccg gggtcgaaaa
      901 gtacagcgtc gtgcgttcgc tgcgcgccat tgaacgctgc gatgtggcgc tgctgatgat
      961 tgatgctgtt gaggggattt ctgcccagga tacgcacatc gccgggtatg ttaaagatga
     1021 atcaaaaagc acggtggtga ttgtgaacaa gtgggacctg ctcgaaaagg acaacttcac
//
LOCUS       DAOTLW010000192         1079 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_113693_length_1079_cov_1.417969, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000192 DAOTLW010000000
VERSION     DAOTLW010000192.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1079)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1079
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_113693_length_1079_cov_1.417969"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1079)
                     /locus_tag="P5516_09740"
     CDS             complement(<1..>1079)
                     /locus_tag="P5516_09740"
                     /inference="COORDINATES: protein motif:HMM:NF013592.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M1 family aminopeptidase"
                     /protein_id="HRT92747.1"
                     /translation="NRTIYDLDLNLDYAQHTASVVQKITYFNRTDQPMHELLLLIPPR
                     YFAGVYSQGSLSGNLVMQSRESGIRTMLSLEPALQPGESTTIQVIFTLLLPKRPGVFG
                     WSEMQTNLMDWFPIIPPFDPASGWLVNERVVDASNTIVGEYLSSETADFSLRLRLTGS
                     AQNLIIAASSEGVEKDGNLEYRLPLARSFSLSVSDQFITREADHKGVSIRTYVFPQHH
                     EAGETIIEVAKNALDIYAELFYPYPRKTLSLVSADFLHNMETDGMLLISKGVIDFYRD
                     APLNNLTILVPHEISHQWFYSLVGNDPAKEPWLDETIATYCESLYFERYHPEHLAWWW
                     SNRVDGFKPEGWVNATIYIPGGYSA"
BASE COUNT          234 a          278 c          317 g          250 t
ORIGIN      
        1 aagcgctgta accgcctggg atgtatatgg tcgcatttac ccacccctcg ggtttgaatc
       61 catccacccg gtttgaccac caccacgcca agtgttccgg gtgatagcgc tcaaagtaga
      121 gcgactcgca atatgtggcg atggtttcgt ccagccaggg ctctttggcg ggatcattgc
      181 caaccagact gtaaaaccac tgatgcgaga tctcgtgcgg gacaaggata gtcaggttgt
      241 tgagcggggc gtcgcgataa aaatcgatca cgcctttgct gatcagcagc atgccgtcgg
      301 tttccatatt atgcaggaag tctgctgaaa ccaggctgag cgtcttgcgc ggatagggat
      361 aaaaaagctc cgcgtagatg tccagcgcgt ttttggcgac ctcgatgatc gtctcaccag
      421 cctcatgatg ctgcgggaac acgtaggttc ggatactgac gcccttgtga tctgcttcgc
      481 gcgtgatgaa ctgatcgctg actgacagag aaaaagacct tgccagcggc aggcggtatt
      541 ctaggttgcc gtctttttcc acgccttcac tgctggcagc gattattaga ttttgggcgc
      601 tgccggtcag gcgaaggcgc agagaaaaat cagcggtttc gctgctgaga tactcaccaa
      661 cgatcgtgtt gctcgcgtcc accactctct catttacaag ccaaccactg gcgggatcaa
      721 atggcggaat gatcgggaac cagtccatga ggttggtctg catctccgac cagccaaaaa
      781 ccccagggcg cttgggcagg agcagggtga atataacttg aatggtggtt gattcgccag
      841 gctgcagagc tggttcgagg ctgagcattg tgcggatgcc actctcgcgc gactgcatca
      901 ccagattgcc gctgaggctc ccctgcgaat acaccccggc aaagtagcgc ggtgggataa
      961 gcagcagcag ctcgtgcatt ggctggtcgg ttcggttgaa atacgtgatt ttttgcacga
     1021 cactggcagt gtgctgcgcg tagtcgaggt ttaggtccag atcgtagatg gtgcggttt
//
LOCUS       DAOTLW010000193         1062 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_116408_length_1062_cov_11.265144, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000193 DAOTLW010000000
VERSION     DAOTLW010000193.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1062)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1062
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_116408_length_1062_cov_11.265144"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1062
                     /locus_tag="P5516_09745"
     CDS             <1..>1062
                     /locus_tag="P5516_09745"
                     /inference="COORDINATES: protein motif:HMM:NF013871.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92748.1"
                     /translation="RDLLLRQPSQDLDIVVEGDAIAFVNALVQLYGGRAVVHRRFGTA
                     KWIISEARETLAHALKSDQPLDPQALPERLDLITARTEFYEKPAALPTVESSNIKMDL
                     HRRDFSINTLALRLDEPFFGKLYDFWGGYHDLQNGIIRVLHALSFVDDATRILRALRF
                     AARFEFRIEPRTLSLMQASLSMLNEISGSRLRHEIDLILLEPKVVDIFVAMVRTNVMQ
                     AIHPKLPWSLEIKDRLGYLNTPAAQAEWETTSNEKQLNIRQIFGYIYWLEDLPERDLE
                     RVAARLRLPAKVVQNIRQAAELRTQLSELRKEKPSVITAALEVIDPLVITAVYQSTPE
                     AELREPLRAMMLHYRQIKPT"
BASE COUNT          253 a          299 c          275 g          235 t
ORIGIN      
        1 cgcgatttgc tgctcaggca gcccagccaa gatctggata tcgtcgtaga aggggatgcc
       61 atcgcttttg taaatgccct ggtgcagctt tatggcgggc gggctgtggt gcatcggcgt
      121 tttggcaccg ccaagtggat catctctgaa gcccgtgaaa ctttggcgca cgcgttgaaa
      181 tccgaccagc ctttggaccc tcaagcgctg ccggagagat tggaccttat cacggcgcgc
      241 acagagtttt acgagaagcc cgccgccctg ccgaccgtgg aaagcagcaa cattaaaatg
      301 gacctgcatc ggcgcgattt ctcgatcaac acactggctc tgcgtttgga tgaacctttt
      361 tttggaaagc tgtatgactt ctggggcggt taccatgacc tccaaaacgg catcatccgc
      421 gttctccatg cgctttcttt tgtggatgac gccacacgca tcttacgggc actgcgcttt
      481 gccgctcgct ttgagttccg catcgagccg cgcacactca gcctgatgca agccagcctt
      541 tcgatgctca acgaaatttc cggctcgcgc ttgcgccatg agatagactt gatcctgctc
      601 gaaccgaagg tagtcgacat attcgtcgcc atggtgcgca ccaatgtaat gcaagcgatc
      661 catccgaagc tgccttggag cttagaaatc aaagaccgct taggttatct caacacacca
      721 gcggcacaag cagaatggga gaccacgagc aacgagaagc agctgaacat ccgccaaatt
      781 tttggttaca tttactggct cgaggatctc cccgagcgag atttagaacg cgtcgcagcg
      841 cggttgcgtt tgcccgctaa ggtagtgcaa aatatccgcc aagcggcgga attgcgcacc
      901 cagctttcag agctaagaaa agaaaaacct agcgtcatca ccgctgcact cgaagttatc
      961 gacccattgg tcattactgc ggtctatcag agcactccag aagcagaact gcgggaaccg
     1021 ctgcgtgcaa tgatgcttca ctacaggcaa attaagccaa ca
//
LOCUS       DAOTLW010000194         1052 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_118113_length_1052_cov_12.423270, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000194 DAOTLW010000000
VERSION     DAOTLW010000194.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1052)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1052
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118113_length_1052_cov_12.423270"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            230..>1052
                     /gene="proC"
                     /locus_tag="P5516_09750"
     CDS             230..>1052
                     /gene="proC"
                     /locus_tag="P5516_09750"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HRT92749.1"
                     /translation="MLQEKNIAIIGPGVMGKTLALSLIRTGNLPPEQIAMAGPNSDRL
                     NQLASELGVRTFSDNQAASNDADIIILAVKPQRLDEATQTLKGNISSGKLVISILAGV
                     PISVLEQKLATRCIVRAMPNTPARIGLGITVWTKAADVDEAQQQTAAQIMRTLGEEIF
                     VADEAYLDMATALSGTGPAYVFLFMEAMIDAGVHMGFPRRIAEQLVVQTLRGSTEFYV
                     QSHGHPAELRDDVTSPGGTSAEALYYLEKAGFRTAISRAIWAAYQRGIELGKGISK"
BASE COUNT          262 a          259 c          284 g          247 t
ORIGIN      
        1 catctttagc ggattggcag gcttgctggt ctattttgtt tcgcgtggcg tggaagcttt
       61 ctataaacat tattcggaaa agtaaaatta aatctaccca ataaaatcac gattttgtgg
      121 taccctacga ctagtgctga tacggtgcct gagataaact tcaatgaatt gttgtcagca
      181 tcctgcggaa tccaattcca gtaaaagcgt gcaatcatag gaggttttaa tgctgcaaga
      241 aaagaacatc gcgattatcg gcccaggtgt gatgggaaaa acactcgctt taagcctgat
      301 ccgcaccgga aacctgccac ccgagcagat agccatggct ggtccaaact cagaccggtt
      361 aaaccagctg gcatcagagc ttggtgtgcg cacattttcc gataatcaag ctgcaagcaa
      421 tgacgcagat atcattatcc tagcagtgaa acctcagcgt ctggatgaag ccacccaaac
      481 cttaaagggc aatatatcct cagggaaact ggtgatttcc atcctcgctg gcgtgccaat
      541 ttcggtgctg gaacagaagt tagctacgcg ctgcatcgtg cgcgccatgc ccaatacgcc
      601 tgcccgcatc ggtttaggaa tcacggtgtg gaccaaagct gcagatgtcg atgaggcgca
      661 acagcaaaca gccgctcaaa ttatgcgcac cttgggtgag gagatttttg tggctgatga
      721 agcctatctg gatatggcta cagcgctctc aggcaccgga ccagcttatg tgttcttatt
      781 tatggaagcg atgatcgatg ctggcgtcca catgggcttc ccgcggcgca tcgcggagca
      841 gctggtggtg caaaccttgc gcggaagtac agagttttac gtgcagagcc atgggcatcc
      901 tgcggaattg cgggatgacg tcacctcgcc cggaggtacc tccgcggagg cgttgtacta
      961 cctcgagaaa gctggttttc gcacggcgat ctcgcgcgcg atatgggcag cttaccagcg
     1021 cggtattgag ctgggcaagg gtattagcaa gc
//
LOCUS       DAOTLW010000195         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_118305_length_1051_cov_11.329317, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000195 DAOTLW010000000
VERSION     DAOTLW010000195.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118305_length_1051_cov_11.329317"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="P5516_09755"
     CDS             complement(<1..326)
                     /locus_tag="P5516_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HRT92750.1"
                     /translation="MDDFQSLEKQFDERVIDIARVAKVVKGGRRLSFRVAVVTGDNNG
                     NVGLGIGKASAVPDAMNKAVQRSHKNMRPINMMGTTIAHEVVGTCGGAKVLLKPASPG
                     TGVIAAG"
     gene            complement(339..704)
                     /gene="rplR"
                     /locus_tag="P5516_09760"
     CDS             complement(339..704)
                     /gene="rplR"
                     /locus_tag="P5516_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006784446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HRT92751.1"
                     /translation="MAKKSRNAARLRRHTRVRKNIFGTPERPRMNVYRSLNEIYVQII
                     DDHSGTTLVSASTIDSGLRSAVSGKNKTEQARVVGEEVARRALEKDIKAVIFDRGGFY
                     YTGRVKALAEGARKGGLEF"
     gene            complement(719..>1051)
                     /gene="rplF"
                     /locus_tag="P5516_09765"
     CDS             complement(719..>1051)
                     /gene="rplF"
                     /locus_tag="P5516_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HRT92752.1"
                     /translation="LNNMIVGVDKGFERVLEVNGVGYRAELNGKDLVLNVGYSHPVNF
                     PAPDGIAFEVDTKTRQIKVKGRDVQQVGQVAANIRMIRPPEHYKGTGIRYLGEVVKLK
                     PGKAGKTG"
BASE COUNT          248 a          300 c          217 g          286 t
ORIGIN      
        1 ccagcagcaa taacacccgt acctggggaa gctggtttca agaggacctt tgcgccaccg
       61 caagtaccca caacttcgtg ggcaatcgtt gtgcccatca tgttgatcgg tcgcatgttt
      121 ttatggctgc gctggaccgc tttgttcatc gcatcgggta cagcactggc tttaccaata
      181 cctaaaccga cattaccgtt attgtcgcca gtgacaacag ccacacggaa ggataaacgc
      241 ctgccgcctt taaccacttt ggcaacccgg gcaatatcaa tgacgcgttc gtcaaattgt
      301 ttttctaaag attgaaaatc gtccataatc tcctcagctt agaactccag cccgccctta
      361 cgtgcacctt cagccaatgc tttgaccctg ccagtgtaat aaaaaccacc gcggtcaaat
      421 ataactgctt tgatgtcttt ttccagtgcg cgccgagcta cttcttcgcc aaccacgcgc
      481 gcctgctcgg ttttattttt tcccgaaaca gcagaacgga gtccgctatc gatggtggaa
      541 gctgacacta acgtcgttcc actatgatca tcaataattt gcacataaat ttcattcagg
      601 ctgcgatata cattcatccg cgggcgctca ggggtgccaa atatattctt acgaactctc
      661 gtatggcggc gtaatcgtgc tgcatttctt gatttttttg ccatattctt ttcctcacct
      721 aaccagtctt accagcttta ccgggtttga gcttaacaac ctcacccagg tagcgaatgc
      781 ccgtaccttt ataatgctcc ggcgggcgga tcatacggat atttgccgct acttgcccaa
      841 cctgttggac gtctcggcct tttaccttaa tttggcgcgt ctttgtatcc acctcaaaag
      901 cgatcccatc aggcgctggg aagttgacgg ggtgagaata ccccacattg agcactaaat
      961 cttttccatt cagctcagca cggtaaccaa caccgttcac ttccagcacg cgttcgaagc
     1021 ctttatcaac gccaacaatc atgttgttga g
//
LOCUS       DAOTLW010000196         1051 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_118311_length_1051_cov_7.343373, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000196 DAOTLW010000000
VERSION     DAOTLW010000196.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1051
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118311_length_1051_cov_7.343373"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1051)
                     /locus_tag="P5516_09770"
     CDS             complement(<1..>1051)
                     /locus_tag="P5516_09770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleoside kinase"
                     /protein_id="HRT92753.1"
                     /translation="MNPNAFYLTETPRPTIEAILPDGRIINGKRGAPLKDFMEVIQDQ
                     ADSLIVGAIVDGNLRELTFPLKKDAEVTPLTLSDADGARIYRRSLTFLLEVAFRRCFP
                     GAVLTIDHSVSSGGYYCQVEGVEGFSQTELDRLEATMRDLVDQDIPFEREVVPLEDAI
                     AYFKSVGEDDKVRLLKYRQRQDLVLYSAAGTRNYHHGYMVPSSGYLKWFALEMLNDAF
                     ILRFPRRHAPTQLSPMVHYPTLLKTFRDYGEWLKKLGIPSVGALNDAIEDGHIDEVIL
                     VSEALHEMQISEIAESIDKRKDQVSVILIAGPSSSGKTTFSKRLSIQLLARGITPFAL
                     EMDNFFVDRDKTPLD"
BASE COUNT          242 a          277 c          306 g          226 t
ORIGIN      
        1 tcatccagcg gggttttatc gcggtcaaca aagaagttat ccatctccaa ggcaaagggg
       61 gtgatgccgc gcgccagcag ttgaatgctc aggcgtttgg agaaggtggt cttgcccgag
      121 gaagaaggac ctgcaatcag gataacgctg acttggtctt tgcgcttgtc gatagattcg
      181 gcaatctcgg agatttgcat ttcgtgcagc gcttccgata ccaaaatcac ttcgtcaata
      241 tgcccatctt caatggcatc gttgagcgcg cccaccgaag ggatgcccaa ctttttcaac
      301 cactcaccgt agtcgcgaaa agtcttgagc aaggtggggt aatgcaccat gggtgagagc
      361 tgcgtaggtg catgccggcg cgggaagcgc aggatgaaag cgtcattgag catttccagt
      421 gcaaaccact tgaggtagcc cgatgatggc accatatagc catggtggta gttgcgcgtg
      481 ccggcagcgc tatagagcac cagatcttgc cgctggcggt acttgagcag gcgcacttta
      541 tcatcctcgc ccacggactt gaagtaggcg atggcatctt ccagcggtac tacttcccgc
      601 tcgaagggga tatcctggtc taccagatcg cgcatcgttg cttctaagcg gtccaattca
      661 gtttgcgaga acccttcgac gccctccacc tggcagtaat agccgccgga ggaaacggaa
      721 tggtcaatgg tgaggacagc gcccgggaag cagcgccgaa aagcaacctc gagcaaaaag
      781 gtcagggagc ggcggtaaat gcgcgcacca tcggcatccg aaagcgtcag cggggtgacc
      841 tcagcatctt ttttgagcgg gaaggtcagt tcgcgcaggt taccatccac aatggcaccc
      901 acaatcaggg aatccgcctg atcctggatg acctccatga agtccttaag gggcgcgccg
      961 cgcttgccat tgatgatcct gccatccggc aggatggctt cgatcgttgg gcgtggggtt
     1021 tcagttaaat aaaacgcatt cgggttcatt t
//
LOCUS       DAOTLW010000197         1050 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_118508_length_1050_cov_10.135678, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000197 DAOTLW010000000
VERSION     DAOTLW010000197.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1050
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_118508_length_1050_cov_10.135678"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            48..132
                     /locus_tag="P5516_09775"
     tRNA            48..132
                     /locus_tag="P5516_09775"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:82..84,aa:Leu,seq:cag)
     gene            203..598
                     /locus_tag="P5516_09780"
     CDS             203..598
                     /locus_tag="P5516_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92754.1"
                     /translation="MRAFFGRLKNKLDYKKIGLIAVAVIMFLLVMDLNSRLNELSRLS
                     TQRDQAATVVANLERTLGVLMTQVEYSRSEGAVEEWAYSDGKMVRPGEQLVIPLAPPG
                     TTPQPVLAPTPTLAQVQNWEIWMALILGK"
BASE COUNT          269 a          240 c          269 g          272 t
ORIGIN      
        1 cacaggttga cattcttcag gagtctgtta aaatatccct cgtaattgcc gaagtggcgg
       61 aactggcaga cgcgctacgt tcagggcgta gtgagcatta cgctcgtgag ggttcaaatc
      121 cctccttcgg cacctcaaaa acccgttaac tgacgcgggt ttttttcttg tctgtgttca
      181 gtgattgagg taatattaaa tcatgagagc atttttcggc aggctgaaga acaaactgga
      241 ttataagaaa atcgggctaa ttgctgtcgc ggtgatcatg ttcctgctgg taatggatct
      301 aaacagccgc cttaatgagc tttcacggct ttcaacccag cgcgaccagg cggcaacagt
      361 ggtggcgaac ctggaacgca ctttaggtgt attgatgacc caggttgagt attcccgatc
      421 tgaaggggca gtggaagaat gggcctacag tgatggcaag atggtgcgcc ctggagaaca
      481 actggtcatc ccgcttgccc cgccgggcac caccccgcag cccgtattgg ctccgacgcc
      541 tacgctggcg caggtgcaaa attgggaaat ctggatggcg ctcattcttg gaaaataatc
      601 aacttctgca caagcagaag ttgattttat atgatggatg ggcagattga tgaggtcatc
      661 tgacgcccac tccccgcaga aagacagaca ggattatcct tactttcaaa ggccaggccc
      721 tcgccagtgg tgaaggtttt attcgttggg tattttcgcg tgaatcacac tgcgtggtct
      781 tttcacggtc atcgcaggtc gaccgaatga aaccagggag atactcttat tcattcataa
      841 gcggttttat tagcgctttt catcgtttcc aacccttcat ccactttggg aaaggatata
      901 gatttaatga ggggtaagaa acaagaggac attctcgggc atttctgctg gaatcaacac
      961 tgtacaacca aaacacgctc atatgaagag ccttgaccgt ttgctggttg gataaggaaa
     1021 aaataacaat taacccgctt gtgatttgtg
//
LOCUS       DAOTLW010000198         1046 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_119230_length_1046_cov_3.398587, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000198 DAOTLW010000000
VERSION     DAOTLW010000198.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1046)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1046
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_119230_length_1046_cov_3.398587"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..550)
                     /locus_tag="P5516_09785"
     CDS             complement(<1..550)
                     /locus_tag="P5516_09785"
                     /inference="COORDINATES: protein motif:HMM:NF019845.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HRT92755.1"
                     /translation="MMKAVIFDKNATPEKLILREVEKPVPDDNQVLIEIHAASINAAD
                     YRSMRMGIIPKHRIFGADVAGRVVTAGSAVTALKPGDAVVGDLSGCGFGGFAEYAVAP
                     ASVLALKPENLSFEDAAAVPLAGVTALQGLRDLGKIQPGQRVLICGAGGGVGTFAVQL
                     AKNFGAHVTAVCGPRSVDLVRSL"
     gene            complement(547..>1046)
                     /locus_tag="P5516_09790"
     CDS             complement(547..>1046)
                     /locus_tag="P5516_09790"
                     /inference="COORDINATES: protein motif:HMM:NF025687.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4386 domain-containing protein"
                     /protein_id="HRT92756.1"
                     /translation="TNYDIVNATNVSNEVKNGYLAIVMLMLLLASASRIGFGGSTADA
                     SALQISGALLISAGDWLGQILAVVFSIGALIIYFLFYQTRLIPRWLSGWGFVGAMLYF
                     AAPLMSMFSPQHPALSLGSPLGFLMAPLALQEMVFAVWLIVKGFSLPPVPTTKKQIFA
                     TTGVQ"
BASE COUNT          255 a          301 c          284 g          206 t
ORIGIN      
        1 caagggaacg cacgaggtcc acgctgcgtg ggccgcagac ggcagtaacg tgcgcgccaa
       61 agtttttggc gagctgcact gcgaaggtcc ccactccccc gcccgcgccg cagatgagca
      121 cccgctgccc aggctgaatt ttgcccaggt cgcgcagtcc ctgcagggca gtgacaccgg
      181 cgagggggac tgccgcggca tcttcaaagg agaggttttc cggtttcaat gccagcacgc
      241 tcgcgggtgc gacagcgtat tccgcaaagc cgccgaaccc acagccggag agatccccaa
      301 ccactgcgtc acccggtttc aacgcggtca ctgctgagcc tgcggttacc acgcgcccgg
      361 caacatccgc gccaaagatg cggtgttttg ggatgatacc catgcgcata gagcgatagt
      421 cggcggcatt gatcgaagcc gcgtggatct cgatcagcac ctggttatcg tcagggacgg
      481 gtttctccac ctcgcgcagg ataagttttt ccggagtggc gttcttatca aaaattactg
      541 ctttcatcat tgaactcccg ttgtagcaaa tatttgcttt tttgtggtgg gcactggcgg
      601 caggctgaaa ccttttacga tcaaccagac cgcaaatacc atttcctgca gggcaagcgg
      661 ggccatcaag aaccccagcg gtgatccgag cgacaacgcc ggatgctgcg gactgaacat
      721 actcatcaga ggtgcggcga agtacagcat ggctccaacg aaaccccaac cagacagcca
      781 gcgagggatc aaccgagtct ggtaaaagag aaagtagatg atcaaagcgc caatgctgaa
      841 aacaacagcc aaaatctgac caagccagtc acccgcgctg attagtaatg cgccggatat
      901 ttgcagcgca gaggcatcag ctgttgatcc cccaaaaccg atccggctgg cagatgcaag
      961 caagagcatc aacatcacaa tggcgaggta gccatttttt acctcatttg aaacgtttgt
     1021 tgcatttact atatcatagt tagttt
//
LOCUS       DAOTLW010000199         1040 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_120338_length_1040_cov_13.764467, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000199 DAOTLW010000000
VERSION     DAOTLW010000199.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1040)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1040
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120338_length_1040_cov_13.764467"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..1016)
                     /locus_tag="P5516_09795"
     CDS             complement(<1..1016)
                     /locus_tag="P5516_09795"
                     /inference="COORDINATES: protein motif:HMM:NF012235.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HRT92757.1"
                     /translation="MAIGTALVWGLGGSMVIAGVITLGTIIAFASYLAQLYGAFQALV
                     NAPVEFATSLVSFERVFEVLDLEPDIQDPAEPIQLEKVRGQISFEGVWFKYPEGRRGL
                     LQEVYRPNVPELVIEELPIESAIALPLPVEEPRNQAREYALEDINLVIEPKQLVALVG
                     PSGAGKTTLTYLMLRLYDPTRGVVNLDGHDLRSLSRKDLTNAIGMVTQEPYLFHDTIL
                     ANLLYAKPDATREEVERVARTANIHDFISALPDGYLTVVGERGYRMSGGEKQRLALAR
                     VLLKNPPIMILDEATSSLDSESEALIQEAMARVFTERTSVVIAHRLSTILSADVIFVM
                     DRGR"
BASE COUNT          245 a          305 c          268 g          222 t
ORIGIN      
        1 atccgcccgc gatccatcac gaaaatcaca tccgccgaga gaatcgtgct caggcggtgc
       61 gcaatcacaa cgctggtgcg ttccgtgaat acgcgtgcca tagcttcctg aatgagtgcc
      121 tcggattcgc tatccaaaga gctcgtggct tcatccagga tcataatcgg cgggttcttg
      181 agcagcaccc gcgccagtgc taagcgctgc ttttcgccgc cgctcatgcg gtagccgcgc
      241 tcccccacta cggtgaggta accatccggc agtgcgctaa taaaatcatg gatattggca
      301 gtgcgtgcca cgcgctccac ctcctctcgg gtagcatcgg gtttagcgta gagcaggttt
      361 gccaaaatag tatcgtggaa aagatagggt tcctgtgtga ccatgccaat ggcgttggtg
      421 agatccttgc ggcttaggct gcgcaggtca tgcccatcca aattgacgac gccgcgggtc
      481 gggtcgtata ggcgcagcat aaggtaagtg agtgtggttt tcccagcgcc gctggggcca
      541 accaacgcaa ctaactgttt aggttcaatc accaaattga tatcttctaa ggcatactct
      601 ctggcttggt tacggggttc ttccaccggc agcggcaagg caatcgcgct ctcaatgggc
      661 agctcttcga tcaccagttc agggacgttc gggcggtaga cttcttgcaa taagcctctc
      721 ctgccctcag gatacttaaa ccagacgccc tcgaagctga tttgcccgcg caccttctcc
      781 agctgaatag gctcagccgg atcttggata tcaggctcca ggtccaatac ctcaaataca
      841 cgctcaaaac tgaccagcga ggtagcaaac tccacaggag cattgaccag cgcttgaaag
      901 gcgccataga gctgagctaa gtaactcgca aaagcaataa tggtgcccaa tgtgatcacg
      961 ccggcaatga ccatactgcc tcctaagccc caaaccagcg ctgtcccaat tgccatcagg
     1021 acgctcagca gcaccataaa
//
LOCUS       DAOTLW010000200         1039 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_120550_length_1039_cov_4.795732, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000200 DAOTLW010000000
VERSION     DAOTLW010000200.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1039)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1039
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_120550_length_1039_cov_4.795732"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            461..952
                     /locus_tag="P5516_09800"
     CDS             461..952
                     /locus_tag="P5516_09800"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRT92758.1"
                     /translation="MVFYLLVGMGILACAILSVLSRRLLISAIWLALTSALVAVMIYL
                     LGAPHIAVIELSVGAGLVTVLFVFAINIAGEDISVLKSLIPMPVAVGAVALAVGLGLV
                     LILRANPVAGLTAPAPERSVILWQSRYVDLLLQVVLIFAGVLGVIGLLADKVKPHYEE
                     ETK"
BASE COUNT          222 a          250 c          267 g          300 t
ORIGIN      
        1 accaaggctg atgctgacgg ctcgtttcat ttgctacctc gatgtttgat aagatagtcg
       61 aacttcgttt atttatacga ctttgatttg atgaccactt attaaaacac ggcaaccgcg
      121 ttttggaggt gaattgttgt cacgaaatta tactgctact cttcctgaag gtttcaatca
      181 acagaaataa tcatcaacgc taatggtttg cgccagttct ccggtaccca agattccctt
      241 gccttccttg tccatgccgt taaaatgctg aagccacgcg ctggcatgca accagcgaca
      301 gctctgtatg acgataatag aattacggaa tataatagac aaggttatca ggcaaaaaaa
      361 gagcacatat aaaggataat ggtgattgtc aacgcctgcc gttagcaggc gcttaccaca
      421 aaaaaaatgg tttgaaatcc ttgttttatt ggaggagggt atggtctttt atcttttagt
      481 gggaatgggc atcctggcct gtgccatcct ttctgtcctt tcccgccgct tgttaatttc
      541 cgccatctgg ctggcattga ccagcgcgct ggtggcggtg atgatctacc tgttaggagc
      601 gccgcatatt gcggtgattg aattgagcgt cggagcgggg ttggtcaccg tcctttttgt
      661 ctttgcgatc aatatcgcag gcgaagatat ctcagtgctg aagtcgctga tccccatgcc
      721 cgtggcggtt ggcgctgtgg cgctggcggt ggggctgggg ctggtgctca tcctgcgcgc
      781 gaaccctgtt gcgggtctca ccgcccctgc tcctgagaga tccgttatcc tgtggcagtc
      841 acgttatgtt gacctgctgc tccaggttgt gctcatcttc gccggagtat taggggtgat
      901 cgggttgctc gctgataagg tcaaacccca ttatgaggag gagacaaaat gaacctgtct
      961 ctccctctga ttattctgct tactatcttc tgcctggcgg gtattggcct gtactgcctg
     1021 ctgatcaccc gtaacctga
//
LOCUS       DAOTLW010000201         1036 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_121118_length_1036_cov_4.555556, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000201 DAOTLW010000000
VERSION     DAOTLW010000201.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1036)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1036
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_121118_length_1036_cov_4.555556"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            458..949
                     /locus_tag="P5516_09805"
     CDS             458..949
                     /locus_tag="P5516_09805"
                     /inference="COORDINATES: protein motif:HMM:NF024641.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4040 domain-containing protein"
                     /protein_id="HRT92759.1"
                     /translation="MVFYLLVGMGILACAILSVLSRRLLISAIWLALTSALVAVMIYL
                     LGAPHIAVIELSVGAGLVTVLFVFAINIAGEDISVLKSLIPMPVAVGAVALAVGLGLV
                     LILRANPGAVLTAPAPEKSVILWQSRYVDLLLQVVLIFAGVLGVIGLLADKVKPHYEE
                     EIK"
BASE COUNT          222 a          252 c          269 g          293 t
ORIGIN      
        1 accaaggctg atgctgacgg ctcgtttcat ttgctacctc gatgtttgat aagatggtag
       61 aactttgatt atttatgact ttgatttgat gatcacatat taaaacacga caacggcgct
      121 ttggaggtga attattgtca cgaaattata ctgctactct tcctgaagct ttcaaccaac
      181 ggaaggatcg acaacgctta atggtttgtg ccagttctcc ggtacccaag attcccttgc
      241 cttccttgcc catgccgata aaatactgaa gccgcgcgct ggcatgcaac cagcgccagc
      301 tcggctatga cgataataga attacggaat ataatagaca aggttatcag gcaaaaagag
      361 cacacataaa ggataatggt ggttgtaaac gtctaccgta agcaggcgct caccacaaaa
      421 aattttgttt gaaatccttg ttttattgga ggagggtatg gttttttatc tattagtggg
      481 aatgggcatc ctggcctgtg ccatcctttc cgtcctttcc cgccgcttgt taatttccgc
      541 catctggctg gcattgacca gcgcgctggt ggcggtgatg atctacctgt taggagcgcc
      601 gcatattgcg gtgattgaat tgagcgtcgg agcggggttg gtcaccgtcc tttttgtctt
      661 tgcgatcaat atcgcaggcg aagatatctc agtgctgaag tcgctgatcc ccatgcccgt
      721 ggcggttggc gctgtggcgc tggcggtagg gctggggctg gtgctcatcc tgcgcgcgaa
      781 ccctggcgcg gttctcaccg cccctgctcc tgagaaatcc gttatcctat ggcagtcacg
      841 ttatgttgac ctgctgctcc aggttgtgct catcttcgcc ggggtattgg gggtgattgg
      901 gttgctcgct gataaggtca aaccccatta tgaggaggag ataaaatgaa cctgcccctc
      961 cctctgatta ttctgcttac catcttctgc ctggcgggta ttggcctgta ctgcctgctg
     1021 atcacccgta acctga
//
LOCUS       DAOTLW010000202         1023 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_123633_length_1023_cov_7.043388, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000202 DAOTLW010000000
VERSION     DAOTLW010000202.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1023
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_123633_length_1023_cov_7.043388"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            88..>1023
                     /locus_tag="P5516_09810"
     CDS             88..>1023
                     /locus_tag="P5516_09810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92760.1"
                     /translation="MNLLERASAAVLSAAAVLGRTFYIDVVEEMTGVTDEALVAALNE
                     LSLKGFISANLEDRTEGSYVFSREVEREITLDQLGPAEKRRLHLRAAKALLSRRPRQP
                     AFAKEIALHFETAGETQPAVKAWLEAGRHARSIFSKDETYLSYGRALELINKLPVGYD
                     ESVIYQVVNEWGNYAHDHDDSRTCEKIYQRCLEVGEATHSLLLIGTGLSGLGRAADFL
                     YEYEKSAEYFQRAIFYLSNTEYDAELIKALSRLGIMQFGMDEYTRAYELLTDALKRDP
                     ATKDQDSLDNRVNILSYICFLHIFFGEPGKAEKIAT"
BASE COUNT          262 a          231 c          289 g          241 t
ORIGIN      
        1 cagcagaaaa atatatccag tgatcagctt tctgattttg gcgcctgttc tccgctagag
       61 tccatcgtca ggctggtcag aggaaagatg aacctgctgg aaagggcttc tgcagcggtg
      121 ctgtcagcgg cggcagtttt agggaggact ttctacattg atgtcgttga agagatgacc
      181 ggcgtaactg acgaagccct ggtggcggct ttaaatgagt tgagcctgaa aggctttata
      241 agcgccaatc ttgaggacag gacggagggg agttatgtgt tcagccgcga ggtggaacgc
      301 gaaataaccc tggaccaatt gggccctgca gaaaagaggc gcctgcatct acgggcggcg
      361 aaagcgctgc tcagccgccg tcccaggcag cctgcgtttg caaaagaaat cgcccttcat
      421 tttgaaactg ctggagagac acagccggct gtgaaggcgt ggttggaagc aggaaggcac
      481 gcgcgttcaa ttttttcaaa agacgaaact tatctttcat acgggcgggc tttggagttg
      541 atcaataagt taccggtagg atatgacgag tccgtcattt accaggtggt gaatgagtgg
      601 ggaaactatg cccacgacca cgatgattcg cgcacctgcg aaaaaattta tcagcgctgc
      661 ctggaggttg gagaagcaac gcacagcctg ctgcttattg gtaccgggtt aagcggtttg
      721 ggtagagcgg cagatttctt atatgaatat gaaaaatcag cggagtattt tcagcgggcc
      781 atcttttacc tgtccaacac cgagtacgac gcggaactca tcaaagcctt atctcgattg
      841 gggataatgc agtttgggat ggatgaatac acacgtgcat atgaactgct cacggacgct
      901 ttgaaacgcg atcccgcgac gaaagatcag gattcacttg ataaccgcgt gaacatcctt
      961 tcttacatct gtttcctgca cattttcttt ggagagcctg gcaaggctga aaaaatcgcc
     1021 acc
//
LOCUS       DAOTLW010000203         1011 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_126063_length_1011_cov_10.506276, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000203 DAOTLW010000000
VERSION     DAOTLW010000203.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1011)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1011
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_126063_length_1011_cov_10.506276"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..404)
                     /locus_tag="P5516_09815"
     CDS             complement(<1..404)
                     /locus_tag="P5516_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92761.1"
                     /translation="MRFSDQNEIENRFKRRAKQRRRDITWNLLTAVVLMLTIFLVGFY
                     LVLFSNPYVAFNPFPPPTMPVLATALPGTATPLRLPPTWTPTVPPTEEPPLTYTPISA
                     TAPVESTSTASPLPLFTDEPVNQNLPYAIEGEP"
     gene            complement(424..>1011)
                     /locus_tag="P5516_09820"
     CDS             complement(424..>1011)
                     /locus_tag="P5516_09820"
                     /inference="COORDINATES: protein motif:HMM:NF024545.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HRT92762.1"
                     /translation="QAAGKSGFTVTDEEVQARIDRLATDMGGAEKLAEWQSINGYTDE
                     TFRVALKRAIAVAWQRDTIVNNVPETAEQIHARQLFFKNEANAIAALAQLKKGIDFST
                     LARQQDDVLGGDLGWFPRGVLTQAEVEEVVFTMQPGETSEIIASNLGYHIVNVIEREP
                     EHPLSTEARLILQEKALATWIENARAASTIEILVP"
BASE COUNT          213 a          266 c          285 g          247 t
ORIGIN      
        1 ggttctccct cgatggcgta tggcaggttt tggttcaccg gctcgtcggt gaataatggc
       61 agcgggcttg ctgtggaagt tgattccacg ggggcggtgg ctgaaatggg cgtgtaggtt
      121 aacggaggtt cctctgtggg cggcactgtc ggcgtccagg tgggcggcag tctcagtggg
      181 gtcgcggtgc cagggagcgc ggtcgccaga acaggcattg tgggcggggg gaatgggttg
      241 aaagcgacgt aagggttgga aaaaagcacc aggtagaaac caaccaaaaa gatggtcaac
      301 atcagtacca cagcggtgag caggttccag gttatatcac gccgacgttg tttggcgcgg
      361 cgcttaaagc ggttttcgat ttcattttgg tcagaaaaac gcatcgcgca aagccttcgc
      421 taatcacgga acgagtatct caatggtgct ggcagccctg gcgttttcaa tccaggttgc
      481 caaagctttt tcctgtaaaa tcaaacgggc ttctgttgaa agtggatgtt caggctcacg
      541 ctcaataaca ttgacaatat ggtagccgag gttgctggca ataatttcgc tggtctcccc
      601 tggctgcatg gtgaagacca cctcctccac ctctgcttgt gtgagtacac cgcgtgggaa
      661 ccaacccagg tcaccgccaa gcacatcgtc ctgctgccgc gccagggttg aaaagtctat
      721 cccctttttt aattgagcca aagccgcgat cgcatttgct tcatttttaa agaataactg
      781 cctggcgtga atctgctcgg ctgtttcagg cacattattg acgatagtgt cccgctgcca
      841 ggcaaccgca atcgccctct tgagcgccac cctgaaggtt tcatctgtat acccattgat
      901 actttgccat tccgccagtt tctccgcccc gcccatgtcg gtcgccagtc tatcaatacg
      961 cgcttgcacc tcttcatcgg taacggtgaa gccgctcttg ccagcagcct g
//
LOCUS       DAOTLW010000204         1006 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_127129_length_1006_cov_9.130389, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000204 DAOTLW010000000
VERSION     DAOTLW010000204.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1006)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1006
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127129_length_1006_cov_9.130389"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..418)
                     /locus_tag="P5516_09825"
     CDS             complement(<1..418)
                     /locus_tag="P5516_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HRT92763.1"
                     /translation="MEQQVSSFAVKKGLAQMLKGGVIMDVVTPEHARIAEDAGAVAVM
                     ALERVPADIRASGGVARMSDPELILKIMEAVSIPVMAKCRIGHFVEAQILEALGVDYI
                     DESEVLTPADEANHIYKHTFKVPFVCGCRNLGEALRR"
     gene            complement(482..>1006)
                     /locus_tag="P5516_09830"
     CDS             complement(482..>1006)
                     /locus_tag="P5516_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014068.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HRT92764.1"
                     /translation="MKLTGKKIAFLVAEGVEDLEYHVVAMRLQEEGAEVLTAGLDLKP
                     LHGKNGLVITPDTLIKDLKADELFALVLPGGWAPDKLRRYAEVKELVAKVNAQGKTIG
                     IICHGGLVAISAGIVRGKHAIGSLGIKDDLENAGAIWDDRPAFREGNLVWGRVVADIP
                     DFNRELVAALVERA"
BASE COUNT          227 a          311 c          227 g          241 t
ORIGIN      
        1 tgcggcgcaa agcttcgcct aaattgcggc agccacacac aaacggcacc ttgaaggtat
       61 gtttgtagat atgattagct tcatctgctg gggtcaagac ctcggattcg tcgatgtagt
      121 ccacgcctaa agcttctaat atctgtgctt caacaaaatg cccaatgcgg cacttagcca
      181 ttactggaat gctaactgcc tccataattt taaggatcag ttcagggtca ctcatgcggg
      241 ctacgccacc actggcacgg atatccgctg ggacgcgttc gagcgccatc accgccactg
      301 cgccagcatc ttccgcgatg cgcgcatgct caggcgtcac cacatccata atgacaccgc
      361 cttttaacat ctgtgctaac ccctttttca cagcaaaact ggatacttgc tgctccatgc
      421 aacctccaaa cacttaaata ttctcacttg cattgtacca ctgctccggc gttaaggagc
      481 cttatgcacg ctccaccaag gcagccacga gctcacggtt aaaatcggga atatccgcaa
      541 ccacccgccc ccacaccagg ttgccttcgc ggaaagctgg gcggtcatcc caaatggcac
      601 ccgcgttctc taggtcatct ttaatcccca gtgatccaat ggcgtgtttt ccgcgcacaa
      661 tgccagcgga gatggcaacg agcccgccat ggcaaataat accgatggtc ttgccttgcg
      721 cgttcacctt agctaccagt tctttcactt cggcataacg gcgcagctta tctggcgccc
      781 agccgcctgg cagtaccagc gcaaagagct cgtcagcttt caggtccttg atcaaggtat
      841 ccggggtaat caccaagccg tttttgccat gcaagggttt caggtccaag ccggcggtca
      901 gcacctcggc gccttcttct tgcaggcgca tggctaccac gtgatattcc aggtcttcca
      961 cgccttcggc gactaaaaat gcaattttct ttccggttag tttcat
//
LOCUS       DAOTLW010000205         1002 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_127946_length_1002_cov_6.852165, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000205 DAOTLW010000000
VERSION     DAOTLW010000205.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1002)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1002
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_127946_length_1002_cov_6.852165"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            <1..>1002
                     /locus_tag="P5516_09835"
     CDS             <1..>1002
                     /locus_tag="P5516_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012364.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HRT92765.1"
                     /translation="VLTPASEELGVVPLTLKPIYEKRVALKAKTRFYPPDHPMAKTLK
                     ARSAALKWLLVVCFGFLGYKNARYGRIEAHEAVTKGGREVLLRAKEVAEEEGFEVLHM
                     YVDALWLKKKGCSKPEHFEDVMHKISSRTGLAIALDGIYRWVAFLPSKTDPRVPIPNR
                     YFGVFQSGEIKVRGLEARRRDTPRWIAQVQTEMLTCLGQAKTRAELPEALHAAFQVYE
                     QSVRKLKQGQVPPELLVVNGKVSYALEAYKSATASVRAARQLQAAGMQIRPGQRVRFL
                     FMQGEPDVYAWELGGQVDPAKLDQHKYLELLAKAGGSVLLPFGIDPQVLMEWTLKGG"
BASE COUNT          221 a          268 c          310 g          203 t
ORIGIN      
        1 atgtgctcac accagccagc gaagagctgg gcgtggtccc gctgacgctc aagcccatct
       61 acgagaagcg tgtggcactc aaagccaaaa cccgctttta cccacctgac catcccatgg
      121 caaagacatt aaaagcacgc tccgcggcgc taaaatggct gctggtggtt tgctttggtt
      181 ttttgggcta caaaaatgcg cgttacgggc gcattgaagc acacgaggca gttaccaaag
      241 gcgggcgcga ggtgctgctg cgcgctaaag aagtcgccga ggaagaaggc tttgaagtgt
      301 tgcacatgta tgtggatgcc ctttggctga agaaaaaggg ttgcagcaag ccagagcatt
      361 tcgaagatgt gatgcataag atttccagcc gcactgggct ggcgattgcc ctagatggca
      421 tttaccgctg ggtggctttt ttgccctcca aaaccgaccc gcgtgtgccc atccccaacc
      481 gctatttcgg cgtcttccag agcggcgaga tcaaagtgcg cggcttggaa gcccgccggc
      541 gggatacccc gcgctggatc gctcaggtgc aaaccgaaat gctcacttgc ctgggtcagg
      601 caaaaacccg cgccgaattg ccagaggctt tgcacgcagc ttttcaggtg tatgaacaat
      661 ccgtcaggaa gttgaagcaa ggtcaggtgc cgccggagct gttggtggtg aatggcaagg
      721 ttagctacgc cttggaggct tacaagagcg ccacggcttc ggtgcgcgct gcacgccagc
      781 tgcaagctgc cggcatgcag atccgcccgg ggcagcgtgt gcgcttccta tttatgcagg
      841 gcgagccgga tgtatacgct tgggaattag gcgggcaggt ggaccccgct aagctggatc
      901 agcacaagta tctggaattg ctggctaaag ccggcggaag cgtgctgctg ccctttggta
      961 ttgacccgca ggtgctaatg gaatggacgc tcaaaggcgg ca
//
LOCUS       DAOTLW010000206         1000 bp    DNA     linear   ENV 11-JUN-2024
DEFINITION  MAG TPA_asm: Anaerolineaceae bacterium isolate AMR_MDS_2937
            NODE_128401_length_1000_cov_8.608466, whole genome shotgun
            sequence.
ACCESSION   DAOTLW010000206 DAOTLW010000000
VERSION     DAOTLW010000206.1
DBLINK      BioProject: PRJNA925477
            BioSample: SAMN33867961
            Sequence Read Archive: SRR5007140
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineaceae bacterium (wastewater metagenome)
  ORGANISM  Anaerolineaceae bacterium
            Bacteria; Bacillati; Chloroflexota; Anaerolineae; Anaerolineales;
            Anaerolineaceae.
REFERENCE   1  (bases 1 to 1000)
  AUTHORS   Abdulkadir,N., Saraiva,J.P., Zhang,J., Stolte,S., Gillor,O.,
            Harms,H. and Rocha,U.
  TITLE     Genome-centric analyses of 165 metagenomes show that mobile genetic
            elements are crucial for the transmission of antimicrobial
            resistance genes to pathogens in activated sludge and wastewater
  JOURNAL   Microbiol Spectr 12 (3), e0291823 (2024)
   PUBMED   38289113
REFERENCE   2  (bases 1 to 1000)
  AUTHORS   Abdulkadir,N.
  TITLE     Direct Submission
  JOURNAL   Submitted (05-APR-2023) Environmental Microbiology, Helmholtz
            Center for Environmental Research -UFZ, Permoserstr 15, Leipzig
            04318, Germany
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: SPAdes v. 3.11.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina MiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 05/04/2023 15:20:48
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.5
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 1,969
            CDSs (total)                      :: 1,923
            Genes (coding)                    :: 1,915
            CDSs (with protein)               :: 1,915
            Genes (RNA)                       :: 46
            rRNAs                             :: 1, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 1, 1 (5S, 16S)
            partial rRNAs                     :: 2 (23S)
            tRNAs                             :: 39
            ncRNAs                            :: 3
            Pseudo Genes (total)              :: 8
            CDSs (without protein)            :: 8
            Pseudo Genes (ambiguous residues) :: 0 of 8
            Pseudo Genes (frameshifted)       :: 1 of 8
            Pseudo Genes (incomplete)         :: 7 of 8
            Pseudo Genes (internal stop)      :: 1 of 8
            Pseudo Genes (multiple problems)  :: 1 of 8
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1000
                     /organism="Anaerolineaceae bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="NODE_128401_length_1000_cov_8.608466"
                     /isolate="AMR_MDS_2937"
                     /isolation_source="Activated sludge of municipal
                     wastewater treatment plants"
                     /db_xref="taxon:2024896"
                     /environmental_sample
                     /geo_loc_name="Japan"
                     /lat_lon="34.72 N 135.27 E"
                     /collection_date="2015-05-26"
                     /metagenome_source="wastewater metagenome"
                     /note="metagenomic"
     gene            complement(<1..597)
                     /locus_tag="P5516_09840"
     CDS             complement(<1..597)
                     /locus_tag="P5516_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HRT92766.1"
                     /translation="MSAQGSTLLLLLAALIGLFIGLLLSSLSSGKEGKRKSQPPPEIL
                     KEGYGEVARLWYSPGTKKILTEMDGGFYKSVNGLSGEQKAKLKRLIALLNEWLGAEPP
                     VETTPQATVVENTHYQPLPDPVSRENDDFYDKEEGATPVEVAVSPFLPTEEEEADVIS
                     ELQQSLPPEDEEETGYTAPEAAEYTITQQINAILQEMLQ"
     gene            complement(699..>1000)
                     /locus_tag="P5516_09845"
     CDS             complement(699..>1000)
                     /locus_tag="P5516_09845"
                     /inference="COORDINATES: protein motif:HMM:NF018533.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="zinc finger domain-containing protein"
                     /protein_id="HRT92767.1"
                     /translation="YTDEALHLARVHPLEKASSLNYSQAESLLRAIREVLTEGIRRNG
                     ASIDWAYRGGDFQNYFRVYQRNGEKCPLCGDKIERIMVSQRGTHFCPSCQPLEKG"
BASE COUNT          238 a          262 c          236 g          264 t
ORIGIN      
        1 ttgcagcatc tcctgcagaa tggcgtttat ttgctgggtg attgtatact cagcagcttc
       61 cggcgctgtg tacccggtct cctcttcatc ttcaggaggt agagactgtt gcagctcgct
      121 gatcacatcc gcttcttctt cctcggtggg caagaaagga gaaaccgcaa cttcaacagg
      181 ggtcgctcct tcttctttat catagaaatc gtcattttca cggctaacgg ggtcaggcag
      241 cggttggtaa tgcgtattct ccacaacagt tgcctgcgga gttgtttcaa ccggaggttc
      301 cgcccctaac cattcattta acagcgcgat caaccgtttc aacttagctt tctgttcccc
      361 acttaatcca ttaacgctct tgtaaaagcc cccgtccatt tcagtgagga tttttttggt
      421 accgggagag taccacaagc gcgccacttc accatatcct tcttttagaa tttcaggggg
      481 aggttggctt tttcgtttgc cttctttgcc tgaagacaaa ctggagagca acaagccaat
      541 gaacaatcca ataagggccg ccagcagtag aaggagcgta gatccttgag cactcattta
      601 acacctcgaa ataagacctg tgaaaaatag aaatacaata acaccatcat tatccaatga
      661 aaaagtaaat aatgcaataa ccctacaata gtgttttttt agcctttttc cagcggctgg
      721 caagatggac aaaaatgagt tccccgctgg ctgaccatga tgcgttcgat tttgtcgccg
      781 cacaaggggc atttctcgcc attgcgctga tacacccgga agtaattctg gaaatccccg
      841 ccgcggtaag cccagtcgat gctggcaccg ttccgccgaa tcccttctgt tagtacctcg
      901 cggatcgccc gcagcaggga ttctgcctgg ctgtagttga gggaagacgc cttctcaagc
      961 gggtgtacgc gggcgaggtg cagcgcctcg tcggtgtata
//
